A G-protein-coupled neuropeptide Y-like receptor suppresses behavioral and sensory response to multiple stressful stimuli in Drosophila.
Recent studies suggest that human neuropeptide Y (NPY) plays a prominent role in management of stress response and emotion, and higher NPY levels observed in combat-exposed veterans may help coping with posttraumatic stress. Neuropeptide F (NPF), the counterpart of NPY in Drosophila melanogaster, also displays parallel activities, including promotion of resilience to diverse stressors and prevention of uncontrolled aggressive behavior. However, it remains unclear how NPY family peptides modulate physical and emotional responses to various stressors. Here we show that NPFR1, a G-protein-coupled NPF receptor, exerts an inhibitory effect on larval aversion to diverse stressful stimuli mediated by different subtypes of fly and mammalian transient receptor potential (TRP) family channels. Imaging analysis in larval sensory neurons and cultured human cells showed that NPFR1 attenuates Ca(2+) influx mediated by fly TRPA and rat TRPV1 channels. Our findings suggest that suppression of TRP channel-mediated neural excitation by the conserved NPF/NPFR1 system may be a major mechanism for attaining its broad anti-stress function.
Xu
Jie
J
Department of Cellular Biology and Biomedical and Health Sciences Institute, University of Georgia, Athens, Georgia 30602, USA.
Li
Mo
M
Shen
Ping
P
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology; D002461:Cell Line, Transformed; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D006801:Humans; D007814:Larva; D008124:Locomotion / Q000235:genetics / Q000502:physiology; D008164:Luminescent Proteins / Q000235:genetics; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009479:Neuropeptides / Q000378:metabolism; D010147:Pain Measurement / Q000379:methods; D010812:Physical Stimulation / Q000379:methods; D018013:Receptors, Neuropeptide / Q000235:genetics; D017476:Receptors, Neuropeptide Y / Q000235:genetics / Q000502:physiology*; D012677:Sensation / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D018689:Sensory System Agents / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D012919:Social Behavior; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*; D050916:TRPV Cation Channels / Q000378:metabolism; D013873:Thionucleotides / Q000494:pharmacology; D013997:Time Factors; D014162:Transfection / Q000379:methods
D002241:Carbohydrates; D029721:Drosophila Proteins; D008164:Luminescent Proteins; C499701:NPFR protein, Drosophila; D009479:Neuropeptides; D018013:Receptors, Neuropeptide; D017476:Receptors, Neuropeptide Y; D018689:Sensory System Agents; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; D013873:Thionucleotides; C076101:neuropeptide F; C083763:8-bromoguanosino-3',5'-cyclic monophosphorothioate; D015124:8-Bromo Cyclic Adenosine Monophosphate; D006152:Cyclic GMP; D002211:Capsaicin; D002118:Calcium