ABA-regulated G protein signaling in Arabidopsis guard cells: a proteomic perspective.
Signaling cascades mediated by heterotrimeric G proteins are ubiquitous and important signal transduction mechanisms in both metazoans and plants. In the model plant Arabidopsis thaliana, the sole canonical G protein alpha subunit, GPA1, has been implicated in multiple signaling events, including guard cell movement regulated by the plant stress hormone abscisic acid (ABA). However, only a handful of proteins have been demonstrated to be involved in GPA1 signaling to date. Here, we compared the proteome composition of guard cells from wild type Col vs gpa1-4 null mutants with and without ABA treatment using iTRAQ technology to identify guard cell proteins whose abundance was affected by ABA and/or GPA1. After imposition of strict selection criteria, the abundance of two proteins in Col and six proteins in gpa1-4 was found to be affected by ABA in guard cells, and 18 guard cell proteins were quantitatively affected by the mutation of GPA1. On the basis of known functions of the differentially expressed proteins, our data suggest that GPA1 inhibits guard cell photosynthesis and promotes the availability of reactive oxygen species (ROS) in guard cells. These results exemplify how iTRAQ can be used to quantitatively study single cell signaling pathways in Arabidopsis.
Zhao
Zhixin
Z
Biology Department, 208 Mueller Laboratory, Penn State University, University Park, Pennsylvania 16802, USA.
Stanley
Bruce A
BA
Zhang
Wei
W
Assmann
Sarah M
SM
D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism*; D044385:GTP-Binding Protein alpha Subunits / Q000378:metabolism*; D007553:Isotope Labeling; D008954:Models, Biological; D009154:Mutation; D018515:Plant Leaves / Q000737:chemistry / Q000166:cytology; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D011523:Protoplasts / Q000737:chemistry; D017382:Reactive Oxygen Species; D015203:Reproducibility of Results; D015398:Signal Transduction
D044385:GTP-Binding Protein alpha Subunits; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D000040:Abscisic Acid