Assessment of genotoxic effects of benzyl derivatives by the comet assay.
In this study, different concentrations of four benzyl derivatives (benzyl alcohol, benzyl acetate, benzoic acid and benzaldehyde) used as flavour ingredients were investigated for genotoxicity in in vitro. By taking blood from two healthy people comet assay was carried on to investigate the potential health damages of benzyl derivatives. For the evaluation of genotoxic effects, the tail moment and % tail DNA in the treated chemicals were compared to the solvent control, which is distilled water. The alkaline comet assay showed significantly increased tail moment and % tail DNA at 25 and 50 mM concentrations of benzyl alcohol. Benzyl acetate increased both % tail DNA and tail moment at 50 mM concentrations. While % tail DNA was statistically increased at 10 mM and higher concentrations, tail moment has significant difference at 10 and 25 mM concentrations of benzaldehyde. Benzoic acid has apoptotic effects at the concentrations higher than 5 mM, for this reason we tested concentrations less than 5mM (0.05, 0.1, 0.5, 1 and 5 mM). Only the highest concentration of benzoic acid increased both tail moment and % tail DNA.
Demir
Eşref
E
Akdeniz University, Faculty of Arts and Sciences, Department of Biology, Antalya, Turkey.
Kocaoğlu
Serap
S
Kaya
Bülent
B
D017209:Apoptosis / Q000187:drug effects; D001547:Benzaldehydes / Q000633:toxicity; D001555:Benzene Derivatives / Q000145:classification / Q000633:toxicity*; D019817:Benzoic Acid / Q000633:toxicity; D019905:Benzyl Alcohol / Q000633:toxicity; D001593:Benzyl Compounds / Q000633:toxicity; D020552:Comet Assay; D004249:DNA Damage; D005421:Flavoring Agents / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D009153:Mutagens / Q000145:classification / Q000633:toxicity*
D001547:Benzaldehydes; D001555:Benzene Derivatives; D001593:Benzyl Compounds; D005421:Flavoring Agents; D009153:Mutagens; C046412:benzyl acetate; D019817:Benzoic Acid; D019905:Benzyl Alcohol; C032175:benzaldehyde