title: Optimization of supercritical fluid extraction of saikosaponins from Bupleurum falcatum with orthogonal array design.
abstract: Supercritical fluid extraction (SFE) was used to extract saikosaponins a, c and d from the root of Bupleurum falcatum. An orthogonal array design L(9)(3)(4) was employed as a chemometric method for the optimization of the SFE conditions. The effects of four factors including pressure (30-40 MPa), temperature (40-50 degrees C), ethanol concentration (60-100%) and time (2.5-3.5 h) on the yields of saikosaponins were investigated by a preparative SFE system in the SFE mode. Under the optimized conditions, namely 35 MPa of pressure, 45 degrees C of temperature, 80% of ethanol concentration and 3.0 h of time, the yields of saikosaponin c, saikosaponin a, saikosaponin d, total saikosaponins and SFE extract were 0.16, 0.12, 0.96, 1.24 and 16.48 mg/g, respectively. Determinations of the saikosaponins were performed by HPLC.
authors.affiliation: State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, PR China.
authors.forename: Yinshi
authors.initials: Y
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.forename: Jianjie
authors.initials: J
authors.lastname: Bi
authors.forename: Zhengbo
authors.initials: Z
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Zude
authors.initials: Z
authors.lastname: Guo
mesh_terms: D029972:Bupleurum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D000431:Ethanol / Q000737:chemistry; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D011312:Pressure; D012503:Saponins / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors
chemical_list: D012503:Saponins; D000431:Ethanol; D009828:Oleanolic Acid; C025759:saikosaponin D