Quantitative, reagentless, single-step electrochemical detection of anti-DNA antibodies directly in blood serum.
Here we demonstrate the use of redox labeled double- and single-stranded oligonucleotides as recognition probes for the reagentless, single-step, electrochemical detection of anti-DNA antibodies directly in blood serum.
Ricci
Francesco
F
University of Rome Tor Vergata, Dipartimento di Scienze e Tecnologie Chimiche, Via della Ricerca Scientifica, 00133, Rome, Italy.
Adornetto
Gianluca
G
Moscone
Danila
D
Plaxco
Kevin W
KW
Palleschi
Giuseppe
G
D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000737:chemistry / Q000276:immunology*; D001774:Blood Chemical Analysis / Q000379:methods*; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000276:immunology*; D015342:DNA Probes / Q000737:chemistry / Q000276:immunology; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D007118:Immunoassay; D057230:Limit of Detection; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D019095:Point-of-Care Systems; D011487:Protein Conformation; D013997:Time Factors
D000906:Antibodies; D015342:DNA Probes; D006046:Gold; D004247:DNA