Interaction of an essential Escherichia coli GTPase, Der, with the 50S ribosome via the KH-like domain.
Der, an essential Escherichia coli tandem GTPase, has been implicated in 50S subunit biogenesis. The rrmJ gene encodes a methyltransferase that modifies the U2552 residue of 23S rRNA, and its deletion causes a severe growth defect. Peculiarly, overexpression of Der suppresses growth impairment. In this study, using an rrmJ-deletion strain, we demonstrated that two GTPase domains of Der regulate its association with 50S subunit via the KH-like domain. We also identified a region of Der that is critical for its specific interaction with 50S subunit.
Hwang
Jihwan
J
Center for Advanced Biotechnology and Medicine, Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 679 Hoes Lane, Piscataway, NJ 08854, USA.
Inouye
Masayori
M
D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008780:Methyltransferases / Q000235:genetics / Q000502:physiology; D016296:Mutagenesis; D010641:Phenotype; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D054681:Ribosome Subunits, Large, Bacterial / Q000235:genetics / Q000378:metabolism*
D018797:Cell Cycle Proteins; D029968:Escherichia coli Proteins; D008780:Methyltransferases; C102247:rlmE protein, E coli; C515037:Der protein, E coli; D019204:GTP-Binding Proteins