Gold nanoparticles functionalized by gadolinium-DTPA conjugate of cysteine as a multimodal bioimaging agent.
The synthesis and characterization of gold nanoparticles coated with Gd-chelate (Au@GdL), where L is a conjugate of DTPA and cysteine, is described. These particles are obtained by the replacement of citrate from the gold nanoparticle surfaces with gadolinium chelate (GdL). The average size of Au@GdL is 14 nm with a loading of GdL reaching up to 2.9x10(3) per particles, and they demonstrate very high R1 relaxivity (approximately 10(5) mM(-1) s(-1)) as well as X-ray attenuation. The R1 relaxivity per [Gd] is 17.9 mM(-1) s(-1). The present system also exhibits macrophage-specific property, as demonstrated by histological and TEM images as well as CT and MR, rendering itself as a new class of T1 multimodal CT/MR contrast agent.
Park
Ji-Ae
JA
Laboratory of Nuclear Medicine Research, Molecular Imaging Center, Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea.
Kim
Hee-Kyung
HK
Kim
Joo-Hyun
JH
Jeong
Sang-Won
SW
Jung
Jae-Chang
JC
Lee
Gang-Ho
GH
Lee
Jongmin
J
Chang
Yongmin
Y
Kim
Tae-Jeong
TJ
D000818:Animals; D003287:Contrast Media / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D019786:Gadolinium DTPA / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure
D003287:Contrast Media; D006046:Gold; D019786:Gadolinium DTPA; D003545:Cysteine