Protein adsorption and cell adhesion on polyurethane/Pluronic surface with lotus leaf-like topography.
Lotus leaf-like polyurethane/Pluronic F-127 surface was fabricated via replica molding using a natural lotus leaf as the template. Water contact angle measurements showed that both the hydrophobicity of the unmodified polyurethane (PU) surface and the hydrophilicity of the PU/Pluronic surface were enhanced by the construction of lotus leaf-like topography. Protein adsorption on the PU/Pluronic surface without topographic modification was significantly lower than on the PU surface. Adsorption was further reduced when lotus leaf-like topography was constructed on the PU/Pluronic surface. Cell culture experiments with L929 cells showed that adhesion on the PU/Pluronic surface with lotus leaf-like topography was low and adherent cells were spherical and of low viability. The PU/Pluronic surface with lotus leaf-like topography thus appears to be resistant to nonspecific protein adsorption and to cell adhesion, and these effects derive from the both chemical composition and topography. The results suggest a new strategy based on surface topography for the design of antifouling materials.
Zheng
Jun
J
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, 122 Luoshi Road, Wuhan, PR China.
Song
Wei
W
Huang
He
H
Chen
Hong
H
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002470:Cell Survival; D046508:Culture Techniques; D015195:Drug Design; D005340:Fibrinogen / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D020442:Poloxamer / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D013499:Surface Properties; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
D011140:Polyurethanes; D011506:Proteins; D012709:Serum Albumin; D013778:Tetrazolium Salts; D013844:Thiazoles; D020442:Poloxamer; D005340:Fibrinogen; C022616:thiazolyl blue