A novel fluorogenic coumarin substrate for monitoring acid phosphatase activity at low pH environment.
This article described the synthesis and application of 6-chloro-8-fluoro-4-methylumbelliferone phosphate (CF-MUP) in analyzing acid phosphatase activity. Compared to the existing MUP, the new coumarin phosphate, CF-MUP, demonstrateed much higher sensitivity and was more robust for detecting the activity of acid phosphatase than the classic substrate 4-methylumbelliferone phosphate (MUP). The product of enzyme reaction, 6-chloro-8-fluoro-4-methylumbelliferone (CF-MU) possesses strong fluorescence at approximately 450 nm with low pKa (4.7), high fluorescence quantum yield and pH independence in the physiological pH range. This new fluorescence dye, CF-MU, is a convenient tool for assays with buffer pH between 4.5 and 8.
Yang
Desuo
D
Department of Chemistry & Chemical Engineering, Baoji University of Arts & Science, Shaanxi, China.
Li
Zongxiao
Z
Allan Diwu
Yubo
Y
Fu
Hanzhuo
H
Liao
Jinfang
J
Wei
Chunmei
C
Diwu
Zhenjun
Z