title: Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF-3196. Induction, isolation and properties.
abstract: The laccase induction in submerged culture of basidiomycete Cerrena unicolor VKM F-3196 was investigated. Cu(2+) at concentration 0.1 mM was an optimum inducer of C. unicolor laccase. Two isoforms of laccase, namely LacC1 and LacC2, were isolated and characterized. The isoforms were shown to have different physical-chemical and catalytic properties. On the basis of the MALDI TOF MS analysis of tryptic cleavage products of both the proteins and N-terminal amino-acid sequences analysis two isoforms of laccase (LacC1 and LacC2) were classified as products of two different genes.
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Russian Federation, Pushchino, Moscow region, Prospekt Nauki. zoyapozhid@rambler.ru
authors.forename: Zoya Alexandrovna
authors.initials: ZA
authors.lastname: Lisova
authors.forename: Alexander Viktorovich
authors.initials: AV
authors.lastname: Lisov
authors.forename: Alexey Arkadievich
authors.initials: AA
authors.lastname: Leontievsky
mesh_terms: D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D002241:Carbohydrates / Q000737:chemistry; D003470:Culture Media; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D005800:Genes, Fungal; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification; D042845:Laccase / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature
chemical_list: D002241:Carbohydrates; D003470:Culture Media; D005656:Fungal Proteins; D007527:Isoenzymes; D042845:Laccase