title: Carbon nanotubes elicit DNA damage and inflammatory response relative to their size and shape.
abstract: Carbon nanotubes (CNTs) have been one of the most extensively researched and developed nanomaterials. However, little concern has been placed on their safety. The biological effects of CNTs are believed to differ relative to size and shape. Thus, the relationship between the characteristics of CNTs and their safety needs to be evaluated. In this study, we examined the biological effects of different-sized multi-walled CNTs (MWCNTs) and single-walled CNTs (SWCNTs). Long and thick MWCNTs induced the strongest DNA damage while similar SWCNTs caused little effect. Comparison of inflammatory responses of various types of CNTs found that peritoneal CNT administration of long and thick MWCNTs increased the total cell number in abdominal lavage fluid in mice. These results indicate that long and thick MWCNT, but not short and thin MWCNT, cause DNA damage and severe inflammatory effects. These findings might provide useful information for constructing novel CNTs with safety.
authors.affiliation: Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Yamashita
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoshioka
authors.forename: Kazuma
authors.initials: K
authors.lastname: Higashisaka
authors.forename: Yuki
authors.initials: Y
authors.lastname: Morishita
authors.forename: Tokuyuki
authors.initials: T
authors.lastname: Yoshida
authors.forename: Maho
authors.initials: M
authors.lastname: Fujimura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kayamuro
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nabeshi
authors.forename: Takuya
authors.initials: T
authors.lastname: Yamashita
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nagano
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Abe
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Kamada
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kawai
authors.forename: Tadanori
authors.initials: T
authors.lastname: Mayumi
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Norio
authors.initials: N
authors.lastname: Itoh
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Tsunoda
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tsutsumi
mesh_terms: D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000473:pathology; D000818:Animals; D045744:Cell Line, Tumor / Q000187:drug effects; D004249:DNA Damage; D006801:Humans; D007274:Injections, Intraperitoneal; D008175:Lung Neoplasms / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D037742:Nanotubes, Carbon / Q000633:toxicity* / Q000648:ultrastructure; D010316:Particle Size; D010538:Peritonitis / Q000139:chemically induced*
chemical_list: D037742:Nanotubes, Carbon