title: iTRAQ-coupled 2D LC-MS/MS analysis of secreted proteome of HBV-replicating HepG2 cells: potential in biomarkers for prognosis of HCC.
abstract: Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This work aims to investigate the secreted proteins in our recently established cell-based HBV replication system, using isobaric tags for relative and absolute quantitation (iTRAQ)-coupled 2D LC-MS/MS proteomics approach. Such proteins are reflective of early events of HBV infection and thus may have potential as prognostic biomarkers for development of liver diseases.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459, Singapore.
authors.forename: Huixing
authors.initials: H
authors.lastname: Feng
authors.forename: Mingxuan
authors.initials: M
authors.lastname: Wang
authors.forename: Wei Ning
authors.initials: WN
authors.lastname: Chen
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis*; D002853:Chromatography, Liquid; D056945:Hep G2 Cells; D006509:Hepatitis B; D006515:Hepatitis B virus / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis; D013058:Mass Spectrometry; D011379:Prognosis; D020543:Proteome; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D014162:Transfection; D014779:Virus Replication
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; D020543:Proteome