[Determination of sperm DNA integrity by improved sperm chromatin structure assay test].
OBJECTIVE
To search for a simple, quick and accurate method for the sperm DNA integrity test by modifying the conventional sperm chromatin dispersion (SCD) test, and to investigate the reference value range of normal sperm DNA integrity by the improved SCD assay.


METHODS
We modified the conventional SCD test in its procedures, reagents preparing and utensils to be used, compared the improved method with the conventional one, and detected 293 normal semen samples for sperm DNA integrity by the modified SCD test.


RESULTS
An improved SCD test was established, and no statistical differences were found between the results of the modified and conventional methods. The reference value range was determined by the improved SCD assay for normal sperm DNA integrity, which was < 32.58% for abnormal sperm DNA integrity.


CONCLUSIONS
The improved SCD test could detect sperm DNA integrity more quickly, and it reduced the cost in reagents.
Cai
Jing
J
Institute of Reproduction and Genetics, Shenzhen Zhongshan Urological Hospital, Shenzhen, Guangdong 518000, China. caijing0228@sina.com
Liang
Pei-Yan
PY
Yin
Biao
B
Liang
Jin-Ming
JM
Lin
Qi
Q
Zeng
Yong
Y
D000328:Adult; D002843:Chromatin / Q000648:ultrastructure; D004247:DNA / Q000032:analysis*; D005821:Genetic Techniques; D006801:Humans; D008297:Male; D012016:Reference Values; D055101:Semen Analysis; D013081:Sperm Motility / Q000235:genetics; D013094:Spermatozoa / Q000737:chemistry*
D002843:Chromatin; D004247:DNA