[Expression of keratin 8 in carbon tetrachloride-induced liver injury of mice].
To investigate the expression of keratin 8 (K8) in carbon tetrachloride (CCl(4))-induced liver injury of mice.



Forty ICR mice were divided into four groups. CCl(4) 300 microl/kg body weight in olive oil was injected intraperitoneally for 0, 2, 4, 6 weeks in group A, B, C and D, respectively. Mice were sacrificed 3 d after the last injection and then the vital organs were collected and weighed. RT-PCR and immunohistochemistry methods were used to analyze the expression of keratin 8 in the liver.



The ratios of liver and body weight were increased significantly after administration of CCl(4), which were 5.60 %, 6.87%, 7.83 % and 7.76% at 0, 2, 4 and 6 weeks after injection, respectively. The expression of K8 was increased at the 2 w, 4 w and 6 w after CL(4) administration.



The expression of K8 is positively correlated with the liver injury induced by CCl(4). The accumulation of K8 may be involved in the mechanism of liver injury.
Zhao
Si-hai
SH
Laboratory Animal Centre, Xi'an Jiaotong University, School of Medicine, Xi'an, China. sihaizhao@mail.xjtu.edu.cn
Chu
Yong-lie
YL
Liu
En-qi
EQ
D000818:Animals; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D005260:Female; D053533:Keratin-8 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D053533:Keratin-8; C486595:Krt8 protein, mouse; D012333:RNA, Messenger; D002251:Carbon Tetrachloride