Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets.
An ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising.
Prod'hom
Guy
G
Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
Bizzini
Alain
A
Durussel
Christian
C
Bille
Jacques
J
Greub
Gilbert
G
D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology*; D001419:Bacteria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D006801:Humans; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors