Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa.
In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation (membrane lipid disorder), reactive oxygen species (ROS) generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreservation process and reduce the cellular damage. We have confirmed that the isoflavone genistein (10 micromol L(-1)) has antioxidant properties on the frozen-thawed spermatozoa. This results in a decreased ROS production that shows a slight improvement in the sperm motility, and decreases the membrane lipid disorder and DNA damage caused by cryopreservation. These results suggest an effect of genistein on sperm functionality that could be of interest for assisted reproduction treatments using frozen-thawed human spermatozoa, but further studies will be necessary to confirm our findings and to evaluate the possible clinical applications.
Martinez-Soto
Juan Carlos
JC
Department of Physiology, School of Veterinary, Murcia University, Murcia 30100, Spain.
de DiosHourcade
Juan
J
Gutiérrez-Adán
Alfonso
A
Landeras
José Lorenzo
JL
Gadea
Joaquín
J
D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D053938:DNA Fragmentation; D005434:Flow Cytometry; D005615:Freezing; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D008297:Male; D017382:Reactive Oxygen Species / Q000378:metabolism; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology
D000975:Antioxidants; D003451:Cryoprotective Agents; D017382:Reactive Oxygen Species; D019833:Genistein