TNF-alpha-induced aquaporin 9 in synoviocytes from patients with OA and RA.
OBJECTIVE
To determine whether aquaporins (AQPs) are expressed in the synovial tissues of patients with OA and RA, and to examine the patterns of expression in patients with and without hydrarthrosis.


METHODS
AQPs were detected in synovial tissue samples from patients with OA and RA using RT-PCR and immunohistochemistry. Fibroblast-like synoviocytes (FLSs) from patients with OA and RA were cultured and stimulated with TNF-alpha. The expression of AQPs in FLSs was examined using RT-PCR and western blot analyses and the function of aquaglyceroporins was examined by a glycerol uptake assay.


RESULTS
AQP1, -3 and -9 mRNAs were expressed in synovial tissues from patients with OA and RA. AQP1, -3 and -9 proteins were also detected by immunohistochemistry. AQP9 mRNA was expressed more strongly in the synovial tissues of OA patients with hydrarthrosis than those without. AQP9 mRNA and protein expression were strongly induced with TNF-alpha treatment in FLSs, whereas the expression of AQP1 and -3 mRNAs was not induced with TNF-alpha treatment. AQP9 as an aquaglyceroporin was induced by TNF-alpha.


CONCLUSIONS
AQP9 mRNA was detected in synovial tissues from OA and RA patients with hydrarthrosis. AQP9 expression was strongly induced in FLSs with TNF-alpha. Although the functions of AQP1, -3 and -9 in synovial tissues remain to be elucidated, it suggested that AQP9 might be related to the pathogenesis of hydrarthrosis and inflammatory synovitis.
Nagahara
Masashizu
M
Department of Orthopaedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
Waguri-Nagaya
Yuko
Y
Yamagami
Takaya
T
Aoyama
Mineyoshi
M
Tada
Toyohiro
T
Inoue
Katsuhisa
K
Asai
Kiyofumi
K
Otsuka
Takanobu
T
D000368:Aged; D000369:Aged, 80 and over; D020346:Aquaporins / Q000187:drug effects*; D001168:Arthritis / Q000378:metabolism* / Q000473:pathology; D001172:Arthritis, Rheumatoid / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D006833:Hydrarthrosis / Q000378:metabolism* / Q000473:pathology; D007719:Knee Joint; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism / Q000473:pathology; D013583:Synovial Membrane / Q000378:metabolism*; D013585:Synovitis / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
C109669:AQP9 protein, human; D020346:Aquaporins; D014409:Tumor Necrosis Factor-alpha