The progenitor zone of the ventral medial ganglionic eminence requires Nkx2-1 to generate most of the globus pallidus but few neocortical interneurons.
We show that most globus pallidus neurons, but very few neocortical interneurons, are generated from the ventral medial ganglionic eminence and dorsal preoptic area based on fate mapping using an Shh-Cre allele. The Shh-expressing subpallial lineage produces parvalbumin(+) GABAergic neurons, ChAT(+) cholinergic neurons, and oligodendrocytes. Loss of Nkx2-1 function from the Shh-expressing domain eliminated most globus pallidus neurons, whereas most cortical and striatal interneurons continued to be generated, except for striatal cholinergic neurons. Finally, our analysis provided evidence for a novel cellular component (Nkx2-1(-);Npas1(+)) of the globus pallidus.
Flandin
Pierre
P
Department of Psychiatry, University of California at San Francisco, San Francisco, California 94158-2611, USA.
Kimura
Shioko
S
Rubenstein
John L R
JL
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003342:Corpus Striatum / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D005917:Globus Pallidus / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D053823:Hedgehog Proteins / Q000235:genetics; D007395:Interneurons / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D019579:Neocortex / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D055495:Neurogenesis / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D009836:Oligodendroglia / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013687:Telencephalon / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000235:genetics*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D053823:Hedgehog Proteins; C000614414:Nkx2-1 protein, mouse; D009687:Nuclear Proteins; C465570:Shh protein, mouse; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D005680:gamma-Aminobutyric Acid; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine