Intestinal parasites of dogs on the Galapagos Islands.
Dogs on the Galapagos Islands are a unique population created by isolation from the mainland and regulations prohibiting further importation. The effect of infectious agents of these domestic dogs on the indigenous fauna is largely unknown. The purpose of this study was to determine the prevalence of intestinal parasites in dogs on the Galapagos Islands. Fecal samples were collected from 97 dogs presented during neutering campaigns on Santa Cruz (n=51), San Cristobal (n=17), and Isabela (n=29) islands. Feces were evaluated for parasites by microscopic examination after zinc sulfate centrifugation flotation as well as by a commercially available IFA for Cryptosporidium spp. and Giardia spp. Polymerase chain reaction for Cryptosporidium spp. DNA and Giardia spp. DNA was performed on all positive samples to provide the infecting genotypes. Ancylostoma caninum (57.7%) and Toxocara canis (16.5%) were most commonly detected, followed by Giardia spp. (5.2%), Isospora canis (4.1%), Sarcocystis canis (3.1%), and Cryptosporidium spp. (1%). Adequate DNA for sequencing was available for one Giardia spp. which was shown to be assemblage D. Despite being isolated, the dogs on the Galapagos have many of the same enteric parasites detected on the mainland of South America. These dogs are not routinely administered anthelmintics or other drugs, but are often allowed to roam the streets and live in close proximity to humans. Parasite prophylaxis is necessary to decrease the parasite burden within the population and to lessen the risk of spread to humans or other animals also inhabiting the islands.
Gingrich
E N
EN
Colorado State University, College of Veterinary Medicine and Biomedical Sciences, Fort Collins, CO, USA. elise.gingrich@colostate.edu
Scorza
A V
AV
Clifford
E L
EL
Olea-Popelka
F J
FJ
Lappin
M R
MR
D000722:Ancylostoma / Q000302:isolation & purification; D000818:Animals; D003430:Cross-Sectional Studies; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000662:veterinary; D003458:Cryptosporidium / Q000302:isolation & purification; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D004484:Ecuador / Q000453:epidemiology; D005243:Feces / Q000469:parasitology; D005260:Female; D005872:Giardia / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000662:veterinary; D007411:Intestinal Diseases, Parasitic / Q000453:epidemiology / Q000662:veterinary*; D007422:Intestines / Q000469:parasitology; D007549:Isospora / Q000302:isolation & purification; D008297:Male; D012522:Sarcocystis / Q000302:isolation & purification; D017166:Toxocara canis / Q000302:isolation & purification