Cross-reactivity of anti-human, anti-porcine and anti-bovine cytokine antibodies with cetacean tissues.
The cross-reactivity of monoclonal antibodies specific for human, porcine and bovine cytokines was evaluated for three cetacean species: Atlantic spotted dolphins (Stenella frontalis), striped dolphins (Stenella coeruleoalba) and fin whales (Balaenoptera physalus). Formalin-fixed and snap-frozen tissue sections of lung, spleen, liver and mesenteric lymph node were evaluated. T and B lymphocytes and monocytes/macrophages were detected by use of anti-human CD3, IgG and lysozyme polyclonal antibodies (pAbs), respectively. These reagents were successfully applied to both fixed and frozen tissues. Anti-human interleukin (IL)-1 alpha, IL-1 beta, IL-8, tumour necrosis factor (TNF)-alpha and CD25, anti-porcine IL-2, IL-6, IL-10, and anti-bovine IL-4 and interferon (IFN)-gamma antibodies produced immunolabelling in cetacean snap-frozen lymph node sections similar to that obtained with tissue from the species of origin, but they did not react with formalin-fixed tissue sections. Anti-porcine IL-12 pAb did not react with snap-frozen cetacean tissue samples. Macrophages and lymphocytes were the most common cells immunolabelled with the anti-cytokine antibodies. This panel of anti-cytokine antibodies may be used to evaluate cytokine expression in snap-frozen tissue samples from the cetacean species tested.
Jaber
J R
JR
Unidad de Histología y Patología, Instituto de Sanidad Animal (IUSA), Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Spain. jjaber@dmor.ulpgc.es
Pérez
J
J
Zafra
R
R
Herráez
P
P
Rodríguez
F
F
Arbelo
M
M
de los Monteros
A Espinosa
AE
Fernández
A
A
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D016207:Cytokines / Q000276:immunology*; D050617:Fin Whale / Q000276:immunology*; D007150:Immunohistochemistry / Q000379:methods*; D008099:Liver / Q000276:immunology; D008168:Lung / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D013154:Spleen / Q000276:immunology; D050706:Stenella / Q000276:immunology*
D000906:Antibodies; D016207:Cytokines