Bactericidal and virucidal ultrathin films assembled layer by layer from polycationic N-alkylated polyethylenimines and polyanions.
In this work, we designed contact-killing ionically cross-linked polymeric thin films using Layer-by-Layer (LbL) technology. A polycation, N,N-dodecyl,methyl-polyethylenimine, with microbicidal activity was layered with a polyanion, such as poly(acrylic acid), to create LbL films highly effective against both airborne and waterborne Escherichia coli and Staphylococcus aureus (Gram negative and positive bacteria, respectively), as well as influenza A/WSN (H1N1) virus. The dependence of the microbicidal activity on the pH during and post-assembly of LbL film formation, the nature of the polycation and polyanion, the number of layers in the LbL film, and other experimental variables was investigated quantitatively.
Wong
Sze Yinn
SY
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Li
Qing
Q
Veselinovic
Jovana
J
Kim
Byeong-Su
BS
Klibanov
Alexander M
AM
Hammond
Paula T
PT
D000391:Air Microbiology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D004926:Escherichia coli / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D008968:Molecular Conformation; D009975:Orthomyxoviridae / Q000187:drug effects; D011073:Polyamines / Q000737:chemistry / Q000494:pharmacology*; D000071228:Polyelectrolytes; D011094:Polyethyleneimine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D014871:Water Microbiology
D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D011073:Polyamines; D000071228:Polyelectrolytes; D011094:Polyethyleneimine; D011108:Polymers; C525941:N,N-dodecyl,methyl-polyethylenimine