Construction, screening and identification of a phage display antibody library against the Eimeria acervulina merozoite.
A single-chain antibody library against Eimeria acervulina merozoites was constructed by phage display approach. Antibody-displaying phage was selected in four panning rounds against cryopreserved E. acervulina merozoites. Five clones were randomly selected from the fourth panning round, and their nucleotide sequences were aligned and compared to mouse germ-line sequences. Soluble antibody was produced in a non-suppressor Escherichia coli strain, purified by protein A affinity chromatography, and characterized by Western-blotting. Immunofluorescence assay showed localization of the produced recombinant antibody fragment on the surface E. acervulina merozoites. These resultant antibody fragments showed high specificity and binding capacity for soluble antigens and intact fixed merozoites which seems promising as diagnostic, therapeutic and/or vaccine tools against coccidiosis.
Zhao
Yuelan
Y
College of Chinese Traditional Veterinary Science, Agricultural University of Hebei, Dingzhou 073000, China.
Amer
Said
S
Wang
Jianyong
J
Wang
Chengmin
C
Gao
Ying
Y
Kang
Guiying
G
Bao
Yongzhan
Y
He
Hongxuan
H
Qin
Jianhua
J
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D004539:Eimeria / Q000276:immunology*; D052940:Merozoites / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D019151:Peptide Library; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology*
D019151:Peptide Library; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies