title: DESIGN OF LIPOSOMES CONTAINING PHOTOPOLYMERIZABLE PHOSPHOLIPIDS FOR TRIGGERED RELEASE OF CONTENTS.
abstract: We describe a novel class of light-triggerable liposomes prepared from a photo-polymerizable phospholipid DC(8,9)PC (1,2- bis (tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine) and DPPC (1,2-Dipalmitoyl-sn-Glycero-3-Phosphocholine). Exposure to UV (254 nm) radiation for 0-45 minutes at 25 degrees C resulted in photo-polymerization of DC(8,9)PC in these liposomes and the release of an encapsulated fluorescent dye (calcein). Kinetics and extents of calcein release correlated with mol% of DC(8,9)PC in the liposomes. Photopolymerization and calcein release occurred only from DPPC/DC(8,9)PC but not from Egg PC/DC(8,9)PC liposomes. Our data indicate that phase separation and packing of polymerizable lipids in the liposome bilayer are major determinants of photo-activation and triggered contents release.
authors.affiliation: Membrane Structure and Function Section, Nanobiology Program, Center for Cancer Research, NCI-Frederick, Frederick, MD 21702.
authors.forename: Amichai
authors.initials: A
authors.lastname: Yavlovich
authors.forename: Alok
authors.initials: A
authors.lastname: Singh
authors.forename: Sergey
authors.initials: S
authors.lastname: Tarasov
authors.forename: Jacek
authors.initials: J
authors.lastname: Capala
authors.forename: Robert
authors.initials: R
authors.lastname: Blumenthal
authors.forename: Anu
authors.initials: A
authors.lastname: Puri