title: A water-soluble bis(thiosemicarbazone) ligand. a sensitive probe and metal buffer for zinc.
abstract: The first member of a water-soluble family of bis(thiosemicarbazone) ligands is reported. It forms a 1:1 complex with Zn(II) that absorbs intensely in the visible region (lambda(max) = 414 nm; epsilon = 1.8(4) x 10(4) M(-1) cm(-1); pH 7.3). Its affinity for Zn(II) (K(D) = 5.9(3) x 10(-9) M at pH 7.3) was determined by competition with ligand ethylene glycol O,O'-bis(2-aminoethyl)-N,N,N',N'-tetraacetic acid. Its potential application as a chromophoric probe was demonstrated by estimation of the Zn(II) binding affinities of the soluble metal-binding domains of two plant metal-transporting proteins.
authors.affiliation: School of Chemistry and Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Gojko
authors.initials: G
authors.lastname: Buncic
authors.forename: Paul S
authors.initials: PS
authors.lastname: Donnelly
authors.forename: Brett M
authors.initials: BM
authors.lastname: Paterson
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: White
authors.forename: Matthias
authors.initials: M
authors.lastname: Zimmermann
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Xiao
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Wedd
mesh_terms: D008024:Ligands; D008958:Models, Molecular; D010940:Plant Proteins / Q000378:metabolism; D011485:Protein Binding; D012995:Solubility; D013882:Thiosemicarbazones / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D015032:Zinc / Q000032:analysis* / Q000378:metabolism
chemical_list: D008024:Ligands; D010940:Plant Proteins; D013882:Thiosemicarbazones; D014867:Water; D015032:Zinc