Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells.
Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient-oriented disease investigation, drug screening, toxicity tests, and transplantation therapies. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence-activated cell sorting using the anti-satellite cell antibody SM/C-2.6. Furthermore, on transplantation into mdx mice, SM/C-2.6(+) cells exerted sustained myogenic lineage differentiation in injured muscles, while providing long-lived muscle stem cell support. Our data suggest that iPS cells have the potential to be used in clinical treatment of muscular dystrophies.
Mizuno
Yuta
Y
Department of Pediatrics, Graduate School of Medicine, Kyoto University 54 Kawahara-cho, Shogoin, Sakyo-ku Kyoto 606-8507, Japan.
Chang
Hsi
H
Umeda
Katsutsugu
K
Niwa
Akira
A
Iwasa
Toru
T
Awaya
Tomonari
T
Fukada
So-ichiro
S
Yamamoto
Hiroshi
H
Yamanaka
Shinya
S
Nakahata
Tatsutoshi
T
Heike
Toshio
T
D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D051379:Mice; D032342:Muscle Cells / Q000166:cytology; D018482:Muscle, Skeletal / Q000166:cytology*; D012038:Regeneration; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology