title: Rapid and reliable single nucleotide polymorphism-based differentiation of Brucella live vaccine strains from field strains.
abstract: The reliable differentiation of live Brucella vaccine strains from field isolates is an important element in brucellosis control programs. We describe the design, validation, and implementation of a novel single nucleotide polymorphism (SNP)-based typing platform that offers a rapid, reliable, and robust tool to achieve this with improved diagnostic accuracy compared to existing molecular tests. Furthermore, the assays described are designed such that they supplement, and can be run as an intrinsic part of, a previously described assay identifying Brucella isolates to the species level (K. K. Gopaul, C. J. Smith, M. S. Koylass, and A. M. Whatmore, BMC Microbiol. 8:86), giving a comprehensive molecular typing platform.
authors.affiliation: Department of Statutory and Exotic Bacterial Disease, Veterinary Laboratories Agency, Woodham Lane, New Haw, United Kingdom.
authors.forename: Krishna K
authors.initials: KK
authors.lastname: Gopaul
authors.forename: Jessica
authors.initials: J
authors.lastname: Sells
authors.forename: Betsy J
authors.initials: BJ
authors.lastname: Bricker
authors.forename: Oswald R
authors.initials: OR
authors.lastname: Crasta
authors.forename: Adrian M
authors.initials: AM
authors.lastname: Whatmore
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D002002:Brucella / Q000145:classification* / Q000235:genetics*; D002004:Brucella Vaccine / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide
chemical_list: D002004:Brucella Vaccine; D004269:DNA, Bacterial