title: Insulin receptor substrate-2 gene polymorphism: is it associated with endometrial cancer?
abstract: OBJECTIVE
  The G1057D polymorphism in the insulin receptor substrate-2 (IRS-2) gene has been reported to be associated with insulin resistance, obesity and type 2 diabetes. However little is known about its possible association with cancer. To investigate this association, we determined the distribution of its genotypes and frequency of alleles in endometrial cancer patients.
  
  
  METHODS
  The study population consisted of 184 subjects: 44 patients with endometrial cancer and 140 controls without cancer. All the patients were primarily treated with surgical intervention. DNA was extracted from the leucocytes by high pure polymerase chain reaction (PCR) template preparation kit. Genetic polymorphism of IRS-2 G1057D was detected by using PCR-based restriction fragment-length polymorphism.
  
  
  RESULTS
  For IRS-2 G1057D polymorphism, there was a significant difference in genotype distribution and allele frequency between endometrial cancer patients and controls (p < 0.001). The risk for endometrial cancer was 4.87 times higher in the individuals with the IRS-2 DD genotype compared to the GG genotype [95% confidence interval (CI): 1.74-13.63 p = 0.003]. Also individuals with the IRS-2 D allele had a significantly higher risk of endometrium cancer compared with individuals with the IRS-2 G allele, with a relative risk of 2.23 (95% CI: 1.36-3.67, p = 0.001) for cases compared with population controls.
  
  
  CONCLUSIONS
  These results suggest that IRS-2 G1057D polymorphism may be associated with endometrial cancer.
authors.affiliation: Departments of Obstetrics and Gynecology, University of Mersin School of Medicine, Mersin, Turkey. filizcayan@yahoo.com
authors.forename: Filiz
authors.initials: F
authors.lastname: Cayan
authors.forename: Ekrem
authors.initials: E
authors.lastname: Tok
authors.forename: Nurcan
authors.initials: N
authors.lastname: Aras-Ateş
authors.forename: Lokman
authors.initials: L
authors.lastname: Ayaz
authors.forename: Esen
authors.initials: E
authors.lastname: Akbay
authors.forename: Ramazan
authors.initials: R
authors.lastname: Gen
authors.forename: Sevim
authors.initials: S
authors.lastname: Karakaş
authors.forename: Saffet
authors.initials: S
authors.lastname: Dilek
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004247:DNA / Q000097:blood; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: C526297:IRS2 protein, human; D055504:Insulin Receptor Substrate Proteins; D004247:DNA