title: High fat diet induces ceramide and sphingomyelin formation in rat's liver nuclei.
abstract: Obesity increases the risk for hepatic steatosis. Recent studies have demonstrated that high fat diet (HFD) may affect sphingolipid formation in skeletal muscles, heart, and other tissues. In this work we sought to investigate whether HFD feeding provokes changes in content and fatty acids (FAs) composition of sphingomyelin and ceramide at the level of liver and hepatic nuclei. Furthermore, we investigated whether the ceramide formation is related to the activity of either neutral sphingomyelinase (N-SMase) or acidic sphingomyelinase (A-SMase). Three weeks of HFD provision induced pronounced ceramide and sphingomyelin accumulation in both liver and hepatic nuclei, accompanied by increased activity of N-SMase but not A-SMase. Furthermore, a shift toward greater FAs saturation status in these sphingolipids was also observed. These findings support the conclusion that HFD has a major impact on sphingolipid metabolism not only in the liver, but also in hepatic nuclei.
authors.affiliation: Department of Physiology, Medical University of Białystok, ul. Mickiewicza 2C, 15-222 Białystok, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Chocian
authors.forename: Adrian
authors.initials: A
authors.lastname: Chabowski
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Zendzian-Piotrowska
authors.forename: Ewa
authors.initials: E
authors.lastname: Harasim
authors.forename: Bartłomiej
authors.initials: B
authors.lastname: Łukaszuk
authors.forename: Jan
authors.initials: J
authors.lastname: Górski
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D002518:Ceramides / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism; D013109:Sphingomyelins / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D002518:Ceramides; D004041:Dietary Fats; D005227:Fatty Acids; D013109:Sphingomyelins; D013108:Sphingomyelin Phosphodiesterase