title: Bactericidal and virucidal ultrathin films assembled layer by layer from polycationic N-alkylated polyethylenimines and polyanions.
abstract: In this work, we designed contact-killing ionically cross-linked polymeric thin films using Layer-by-Layer (LbL) technology. A polycation, N,N-dodecyl,methyl-polyethylenimine, with microbicidal activity was layered with a polyanion, such as poly(acrylic acid), to create LbL films highly effective against both airborne and waterborne Escherichia coli and Staphylococcus aureus (Gram negative and positive bacteria, respectively), as well as influenza A/WSN (H1N1) virus. The dependence of the microbicidal activity on the pH during and post-assembly of LbL film formation, the nature of the polycation and polyanion, the number of layers in the LbL film, and other experimental variables was investigated quantitatively.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Sze Yinn
authors.initials: SY
authors.lastname: Wong
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Jovana
authors.initials: J
authors.lastname: Veselinovic
authors.forename: Byeong-Su
authors.initials: BS
authors.lastname: Kim
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Klibanov
authors.forename: Paula T
authors.initials: PT
authors.lastname: Hammond
mesh_terms: D000391:Air Microbiology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D004926:Escherichia coli / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D008968:Molecular Conformation; D009975:Orthomyxoviridae / Q000187:drug effects; D011073:Polyamines / Q000737:chemistry / Q000494:pharmacology*; D000071228:Polyelectrolytes; D011094:Polyethyleneimine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D011073:Polyamines; D000071228:Polyelectrolytes; D011094:Polyethyleneimine; D011108:Polymers; C525941:N,N-dodecyl,methyl-polyethylenimine