title: Conditioning film and initial biofilm formation on electrochemical CaCO3 deposition on a metallic net in the marine environment.
abstract: Electrochemical deposition of minerals is a unique technology for artificial reef constructions, relying on calcium carbonate (CaCO3) build-up over metallic structures through electrolysis of seawater. The present study traces the first 72 h following electric current termination on bacterial biofilm build-up on a metallic net covered with CaCO3. 16S rRNA clone libraries indicated a dynamic succession. Proteobacteria and Bacteroidetes were evident at all sampling times while Cyanobacteria appeared only within the first 8 h. A significant increase in total organic carbon (TOC) and total protein was observed after 48 h with a significant correlation (R(2) = 0.74), indicating TOC is a good tool for characterizing initial biofilm formation. 18S rRNA gene sequences obtained 72 h following current termination indicated a significant presence of Cnidarians (51%). Understanding the dynamics among primary bacterial settlers is important because they play a crucial role in driving the colonization of sessile invertebrate communities on artificial, as well as natural surfaces.
authors.affiliation: Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Be'er-Sheva, Israel.
authors.forename: Nachshon
authors.initials: N
authors.lastname: Siboni
authors.forename: Stephane
authors.initials: S
authors.lastname: Martinez
authors.forename: Avigdor
authors.initials: A
authors.lastname: Abelson
authors.forename: Alex
authors.initials: A
authors.lastname: Sivan
authors.forename: Ariel
authors.initials: A
authors.lastname: Kushmaro
mesh_terms: D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D018441:Biofilms / Q000254:growth & development*; D002119:Calcium Carbonate; D000458:Cyanobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D055664:Electrochemical Techniques / Q000379:methods*; D008386:Marine Biology; D008670:Metals; D008969:Molecular Sequence Data; D011157:Population Dynamics; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D017422:Sequence Analysis, DNA; D013499:Surface Properties
chemical_list: D008670:Metals; D002119:Calcium Carbonate