title: Validation of end-point and real-time PCR methods for the rapid detection of soy allergen in processed products.
abstract: This work describes the development and validation of two PCR methods, end-point and real-time PCR, for the detection of soy protein in a wide rage of foodstuffs. These techniques are reliable and sensitive, allowing detection of trace amounts of soybean in processed products. TaqMan real-time PCR was the simpler and more rapid process, with a higher potential for automation and, therefore, currently the most suitable screening method. To verify correct operation of the proposed methodology, ELISA was used for quantitative determination of soy protein. In addition, 35 meat, fish and bakery processed products, which could potentially contain soy but was not declared on the label, were tested for the presence of soy DNA using the proposed methods. The methodologies will be valuable in issues regarding the presence of soy protein in processed products, especially in verifying labelling and security regulations to protect consumer's rights.
authors.affiliation: Area of Molecular Biology and Biotechnology, Vigo, 36310 Pontevedra, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Espineira
authors.forename: Beatriz
authors.initials: B
authors.lastname: Herrero
authors.forename: Juan M
authors.initials: JM
authors.lastname: Vieites
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Santaclara
mesh_terms: D052179:Antigens, Plant / Q000032:analysis*; D001939:Bread / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005396:Fish Products / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D005512:Food Hypersensitivity / Q000517:prevention & control; D005515:Food Labeling; D057141:Food, Preserved / Q000032:analysis; D008461:Meat Products / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D030262:Soybean Proteins / Q000032:analysis* / Q000235:genetics*
chemical_list: D052179:Antigens, Plant; D018744:DNA, Plant; D030262:Soybean Proteins