Identification of preferred carbohydrate binding modes in xenoreactive antibodies by combining conformational filters and binding site maps.
Carbohydrates are notoriously flexible molecules. However, they have an important role in many biochemical processes as specific ligands. Understanding how carbohydrates are recognized by other biological macromolecules (usually proteins) is therefore of considerable scientific value. Interfering with carbohydrate-protein interactions is a potentially useful strategy in combating a range of disease states, as well as being of critical importance in facilitating allo- and xenotransplantation. We have devised an in silico protocol for analyzing carbohydrate-protein interactions. In this study, we have applied the protocol to determine the structures of alphaGal-terminating carbohydrate antigens in complex with a panel of xenoreactive antibodies. The most important feature of the binding modes is the fixed conformation of the Galbeta(1,4)Glc/GlcNAc linkage across all of the binding modes. The preferred conformation of the terminal Galalpha(1,3)Gal linkage varies depending on the antibody binding site topography, although it is possible that some of the antibodies studied recognize more than one Galalpha(1,3)Gal conformation. The binding modes obtained indicate that each antibody uses distinct mechanisms in recognizing the target antigens.
Agostino
Mark
M
Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC 3052, Australia.
Sandrin
Mauro S
MS
Thompson
Philip E
PE
Yuriev
Elizabeth
E
Ramsland
Paul A
PA
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000737:chemistry* / Q000276:immunology*; D001665:Binding Sites; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry* / Q000276:immunology*; D019295:Computational Biology
D000911:Antibodies, Monoclonal; D000941:Antigens; D002241:Carbohydrates