Microfabricated magnetic sifter for high-throughput and high-gradient magnetic separation.
A microfabricated magnetic sifter has been designed and fabricated for applications in biological sample preparation. The device enables high-throughput, high-gradient magnetic separation of magnetic nanoparticles by utilizing columnar fluid flow through a dense array (~5000/mm(2)) of micropatterned slots in a magnetically soft membrane. The potential of the sifter for separation of magnetic nanoparticles conjugated with capture antibodies is demonstrated through quantitative separation experiments with CD138-labelled MACS nanoparticles. Capture efficiencies ranging from 28-37% and elution efficiencies greater than 73% were measured for a single pass through the sifter.
Earhart
Christopher M
CM
Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305.
Wilson
Robert J
RJ
White
Robert L
RL
Pourmand
Nader
N
Wang
Shan X
SX