title: In vivo cyclic loading as a potent stimulatory signal for bone formation inside tissue engineering scaffold.
abstract: In clinical situations, bone defects are often located at load bearing sites. Tissue engineering scaffolds are future bone substitutes and hence they will be subjected to mechanical stimulation. The goal of this study was to test if cyclic loading can be used as stimulatory signal for bone formation in a bone scaffold. Poly(L-lactic acid) (PLA)/ 5% beta-tricalcium phosphate (beta-TCP) scaffolds were implanted in both distal femoral epiphyses of eight rats. Right knees were stimulated (10N, 4Hz, 5 min) five times, every two days, starting from the third day after surgery while left knees served as control. Finite element study of the in vivo model showed that the strain applied to the scaffold is similar to physiological strains. Using micro-computed tomography (CT), all knees were scanned five times after the surgery and the related bone parameters of the newly formed bone were quantified. Statistical modeling was used to estimate the evolution of these parameters as a function of time and loading. The results showed that mechanical stimulation had two effects on bone volume (BV): an initial decrease in BV at week 2, and a long-term increase in the rate of bone formation by 28%. At week 13, the BV was then significantly higher in the loaded scaffolds.
authors.affiliation: Laboratory of Biomechanical Orthopedics, EPFL, CH-1015 Lausanne, Switzerland.
authors.forename: Alireza
authors.initials: A
authors.lastname: Roshan-Ghias
authors.forename: Alexandre
authors.initials: A
authors.lastname: Terrier
authors.forename: Pierre-Etienne
authors.initials: PE
authors.lastname: Bourban
authors.forename: Dominique P
authors.initials: DP
authors.lastname: Pioletti
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology; D001847:Bone Diseases / Q000628:therapy; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry* / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D005269:Femur / Q000187:drug effects / Q000502:physiology / Q000601:surgery; D007719:Knee Joint / Q000187:drug effects / Q000502:physiology / Q000601:surgery; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D010507:Periodicity; D010812:Physical Stimulation / Q000379:methods; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction / Q000502:physiology; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D016474:Weight-Bearing / Q000502:physiology; D055114:X-Ray Microtomography
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; C485817:beta-tricalcium phosphate; D019344:Lactic Acid; C033616:poly(lactide)