Urinary matrix metalloproteinase-8 and -9 activities in type 2 diabetic subjects: A marker of incipient diabetic nephropathy?
Matrix metalloproteinases (MMPs) may play a pathophysiological role in the development of diabetic nephropathy (DN). We hypothesized that urinary MMP activity in patients with type 2 diabetes mellitus (T2DM) is related to a decline in renal function. We determined MMP-2, -8 and -9 activity in 24-h urine collections in relation to risk factors for DN in T2DM patients with (UA, n=27) and without albuminuria (NA, n=48) and controls (CO, n=28). MMP-8 and -9 levels were highest in UA patients (P<0.01). Of UA patients, 93% had at least one MMP increased, compared to 78% of NA patients and 46% of CO (P=0.001). Age, diabetes duration, BMI, systolic blood pressure, fasting plasma glucose, HbA1c and renal function were determinants of MMP-8 and -9 (P<0.05). In summary, MMP-8 and -9 are highest in T2DM UA patients. MMP-9, showed the strongest associations with clinical parameters related to DN.
van der Zijl
Nynke J
NJ
Department of Endocrinology/Diabetes Center, VU University Medical Center, Amsterdam, The Netherlands. nj.vdzijl@vumc.nl
Hanemaaijer
Roeland
R
Tushuizen
Maarten E
ME
Schindhelm
Roger K
RK
Boerop
Jeannette
J
Rustemeijer
Cees
C
Bilo
Henk J
HJ
Verheijen
Jan H
JH
Diamant
Michaela
M
D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology* / Q000652:urine*; D003928:Diabetic Nephropathies / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000652:urine*; D020780:Matrix Metalloproteinase 9 / Q000652:urine*; D008875:Middle Aged; D015999:Multivariate Analysis
D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9