title: Intensity-based analysis of dual-color gene expression data as an alternative to ratio-based analysis to enhance reproducibility.
abstract: BACKGROUND
  Ratio-based analysis is the current standard for the analysis of dual-color microarray data. Indeed, this method provides a powerful means to account for potential technical variations such as differences in background signal, spot size and spot concentration. However, current high density dual-color array platforms are of very high quality, and inter-array variance has become much less pronounced. We therefore raised the question whether it is feasible to use an intensity-based analysis rather than ratio-based analysis of dual-color microarray datasets. Furthermore, we compared performance of both ratio- and intensity-based analyses in terms of reproducibility and sensitivity for differential gene expression.
  
  
  RESULTS
  By analyzing three distinct and technically replicated datasets with either ratio- or intensity-based models, we determined that, when applied to the same dataset, intensity-based analysis of dual-color gene expression experiments yields 1) more reproducible results, and 2) is more sensitive in the detection of differentially expressed genes. These effects were most pronounced in experiments with large biological variation and complex hybridization designs. Furthermore, a power analysis revealed that for direct two-group comparisons above a certain sample size, ratio-based models have higher power, although the difference with intensity-based models is very small.
  
  
  CONCLUSIONS
  Intensity-based analysis of dual-color datasets results in more reproducible results and increased sensitivity in the detection of differential gene expression than the analysis of the same dataset with ratio-based analysis. Complex dual-color setups such as interwoven loop designs benefit most from ignoring the array factor. The applicability of our approach to array platforms other than dual-color needs to be further investigated.
authors.affiliation: Neuroregeneration Laboratory, Netherlands Institute for Neuroscience, Meibergdreef 47, 1105 BA Amsterdam, the Netherlands. k.bossers@nin.knaw.nl
authors.forename: Koen
authors.initials: K
authors.lastname: Bossers
authors.forename: Bauke
authors.initials: B
authors.lastname: Ylstra
authors.forename: Ruud H
authors.initials: RH
authors.lastname: Brakenhoff
authors.forename: Serge J
authors.initials: SJ
authors.lastname: Smeets
authors.forename: Joost
authors.initials: J
authors.lastname: Verhaagen
authors.forename: Mark A
authors.initials: MA
authors.lastname: van de Wiel
mesh_terms: D000704:Analysis of Variance; D001921:Brain; D002460:Cell Line; D016000:Cluster Analysis; D003116:Color; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D015603:Keratinocytes; D008962:Models, Theoretical; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results