Synthesis and AFM characterization of poly(dG)-poly(dC)-gold nanoparticle conjugates.
In the present work, we have synthesized conjugates between the 5 nm gold nanoparticles (Au-NP) and 5' thiol-functionalized, 700 bp poly(dG)-poly(dC). We have completely separated and purified to homogeneity conjugates bearing different number of poly(dG)-poly(dC) molecules per Au-NP by electrophoresis and HPLC. The conjugates were directly visualized by atomic force microscopy. We have demonstrated that Au NP-bound poly(dG)-poly(dC) can be considerably extended by Klenow exo(-) polymerase in the presence of dCTP and dGTP.
Zikich
Dragoslav
D
Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, The Center for Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, 69978.
Borovok
Natalia
N
Molotsky
Tatiana
T
Kotlyar
Alexander
A
D002851:Chromatography, High Pressure Liquid; D004587:Electrophoresis, Agar Gel; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010316:Particle Size; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D013499:Surface Properties
D011089:Polydeoxyribonucleotides; C016681:poly(dG).poly(dC); D006046:Gold