Effects of resveratrol supplementation on cryopreservation medium of human semen.
OBJECTIVE
To analyze oxidative stress markers and seminal standard parameters after using resveratrol (0.1, 1.0, and 10.0 mM), an important antioxidant, in the cryopreservation of human semen.


METHODS
In vitro prospective study.


METHODS
Institutional study.


METHODS
Infertile and fertile men.


METHODS
None.


METHODS
Levels of thiobarbituric acid-reactive species (TBARS), superoxide dismutase (SOD), and catalase (CAT) activities and spermatozoa concentration, motility, and morphology.


RESULTS
Increased TBARS levels were observed in the post-thawing semen in both fertile and infertile men. Infertile men had lower CAT and SOD activities in prefreezing and post-thawing samples when compared with fertile men. The addition of resveratrol in all the concentrations assayed was able to prevent post-thawing lipoperoxidation in both fertile and infertile men. However, this effect was not dose dependent. The cryopreservation process was not able to change sperm concentration or morphology. However, a decrease in sperm motility was observed in both the fertile and infertile men. The addition of resveratrol was not able to prevent this effect.


CONCLUSIONS
Resveratrol avoids oxidative damages induced by the cryopreservation of human semen, but it is not able to restore the decrease in sperm motility.
Garcez
Marcia E
ME
Institute of Biotechnology, University of Caxias do Sul, Brazil.
dos Santos Branco
Cátia
C
Lara
Luana Venturin
LV
Pasqualotto
Fabio F
FF
Salvador
Mirian
M
D000328:Adult; D000975:Antioxidants / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D012661:Semen / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013267:Stilbenes / Q000494:pharmacology*; D055815:Young Adult
D000975:Antioxidants; D003451:Cryoprotective Agents; D017382:Reactive Oxygen Species; D013267:Stilbenes; D002374:Catalase; D000077185:Resveratrol