Characterization of magnetite nanoparticles for SQUID-relaxometry and magnetic needle biopsy.
Magnetite nanoparticles (Chemicell SiMAG-TCL) were characterized by SQUID-relaxometry, susceptometry, and TEM. The magnetization detected by SQUID-relaxometry was 0.33% of that detected by susceptometry, indicating that the sensitivity of SQUID-relaxometry could be significantly increased through improved control of nanoparticle size. The relaxometry data were analyzed by the moment superposition model (MSM) to determine the distribution of nanoparticle moments. Analysis of the binding of CD34-conjugated nanoparticles to U937 leukemia cells revealed 60,000 nanoparticles per cell, which were collected from whole blood using a prototype magnetic biopsy needle, with a capture efficiency of >65% from a 750 µl sample volume in 1 minute.
Adolphi
Natalie L
NL
Senior Scientific, LLC, 11109 Country Club NE, Albuquerque, New Mexico 87111 USA.
Huber
Dale L
DL
Jaetao
Jason E
JE
Bryant
Howard C
HC
Lovato
Debbie M
DM
Fegan
Danielle L
DL
Venturini
Eugene L
EL
Monson
Todd C
TC
Tessier
Trace E
TE
Hathaway
Helen J
HJ
Bergemann
Christian
C
Larson
Richard S
RS
Flynn
Edward R
ER