title: Orientation control of photo-immobilized antibodies on the surface of azobenzene-containing polymers by the introduction of functional groups.
abstract: In our photo-induced immobilization technique for an antibody (IgG) using azopolymers, the introduction of COOH and NMe(2) into the azopolymers, which can introduce surface charges, strongly affected the immobilization properties such as the efficiency of immobilization and the activity of the immobilized IgG (i.e., the orientation of the immobilized IgG). The introduction of COOH promoted a more active orientation of the immobilized IgG. The orientation was determined during the adsorption process onto the azopolymer surface in solution before photo-immobilization, and was maintained during the photo-immobilization. The surface charge of the azopolymer appears to be an important factor for IgG orientation, which involves electrostatic interactions between its Fab and the azopolymer surface.
authors.affiliation: Toyota Central Research and Development Laboratories, Incorporated, Nagakute, Aichi 480-1192, Japan. mouri@mosk.tytlabs.co.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Mouri
authors.forename: Taiji
authors.initials: T
authors.lastname: Ikawa
authors.forename: Mamiko
authors.initials: M
authors.lastname: Narita
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Hoshino
authors.forename: Osamu
authors.initials: O
authors.lastname: Watanabe
mesh_terms: D000327:Adsorption; D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000528:radiation effects; D001391:Azo Compounds / Q000737:chemistry*; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D008722:Methods; D055668:Photochemical Processes; D011108:Polymers / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D055910:Antibodies, Immobilized; D001391:Azo Compounds; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D011108:Polymers; C009850:azobenzene