New efficient recombinant expression system to engineer Candida antarctica lipase B.
Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme.
Emond
Stéphane
S
Ingénierie des Systèmes Biologiques et des Procédés, Université de Toulouse, INSA, UPS, INP, 135 Avenue de Rangueil, F-31077 Toulouse, France.
Montanier
Cédric
C
Nicaud
Jean-Marc
JM
Marty
Alain
A
Monsan
Pierre
P
André
Isabelle
I
Remaud-Siméon
Magali
M
D005656:Fungal Proteins; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D008049:Lipase / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D025062:Yarrowia / Q000235:genetics*
D005656:Fungal Proteins; D008049:Lipase; C431349:lipase B, Candida antarctica