Production, refining, structural characterization and fermentability of rice husk xylooligosaccharides.
Oligosaccharides produced by hydrothermal processing of rice husks (xylooligosaccharides and glucooligosaccharides) were refined by membrane processing (operating in diafiltration and concentration modes), subjected to xylanase treatment to reduce the average molar mass, and subjected to further purification by ultrafiltration (operating in concentration mode) and ion exchange. The purified products were assayed for composition, molar mass distribution and structural characterization by HPLC, HPAEC-PAD, HPSEC, MALDI-TOF-MS and NMR ((1)H and (13)C). The fermentability of the purified product by fecal inocula was assessed on the basis of the time courses of pH and oligosaccharide concentrations. Succinate, lactate, formiate, acetate, propionate and butyrate were the major products resulting from fermentation experiments.
Gullón
Patricia
P
Department of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas, Ourense, Spain.
González-Muñoz
María Jesús
MJ
van Gool
Martine Paula
MP
Schols
Henk Arie
HA
Hirsch
Ján
J
Ebringerová
Anna
A
Parajó
Juan Carlos
JC
D005285:Fermentation; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D012275:Oryza / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009844:Oligosaccharides