Action of 6-amino-3-pyridinols as novel antioxidants against free radicals and oxidative stress in solution, plasma, and cultured cells.
Free radical-mediated lipid peroxidation has been implicated in the pathogenesis of various diseases. Lipid peroxidation products are cytotoxic and they modify proteins and DNA bases, leading eventually to degenerative disorders. Various synthetic antioxidants have been developed and assessed for their capacity to inhibit lipid peroxidation and oxidative stress induced by free radicals. In this study, the capacity of novel 6-amino-2,4,5-trimethyl-3-pyridinols for scavenging peroxyl radicals, inhibiting plasma lipid peroxidation in vitro, and preventing cytotoxicity induced by glutamate, 6-hydroxydopamine, 1-methyl-4-phenylpyridium (MPP(+) ), and hydroperoxyoctadecadienoic acid was assessed. It was found that they exerted higher reactivity toward peroxyl radicals and more potent activity for inhibiting the above oxidative stress than alpha-tocopherol, the most potent natural antioxidant, except against the cytotoxicity induced by MPP(+). These results suggest that the novel 6-amino-3-pyridinols may be potent antioxidants against oxidative stress.
Omata
Yo
Y
Health Technology Research Center, National Institute of Advanced Industrial Science & Technology, Osaka 563-8577, Japan.
Saito
Yoshiro
Y
Yoshida
Yasukazu
Y
Jeong
Byeong-Seon
BS
Serwa
Remigiusz
R
Nam
Tae-gyu
TG
Porter
Ned A
NA
Niki
Etsuo
E
D015655:1-Methyl-4-phenylpyridinium / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D019610:Cytoprotection; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D016627:Oxidopamine / Q000378:metabolism; D016716:PC12 Cells; D011728:Pyridones / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D024502:alpha-Tocopherol / Q000378:metabolism
D000975:Antioxidants; D005231:Fatty Acids, Unsaturated; D016166:Free Radical Scavengers; D011728:Pyridones; C027369:octadecadienoic acid; D018698:Glutamic Acid; D016627:Oxidopamine; D024502:alpha-Tocopherol; D015655:1-Methyl-4-phenylpyridinium