title: Secondary metabolites from anti-insect extracts of endophytic fungi isolated from Picea rubens.
abstract: The extracts of a selection of 150 foliar fungal endophytes isolated from Picea rubens (red spruce) needles were screened by LC-MS and assayed for toxicity. Three of these strains that were toxic to the forest pest Choristoneura fumiferana (eastern spruce budworm) in dietary bioassays were selected for further study. Their culture extracts were analyzed by LC-NMR spectroscopy, and the major metabolites were isolated by LC-MS-SPE or PTLC/column chromatography and characterized. The structures were elucidated by spectroscopic analyses including 2D NMR, HRMS and by comparison to literature data. Compounds 1 and 5-7 are hitherto unknown whereas compounds 2 and 3 are natural products described for the first time. Compound 4 is reported for the first time as a fungal metabolite and 8-9 were identified as known fungal metabolites in genera.
authors.affiliation: Ottawa-Carleton Institute of Chemistry, Carleton University, Ottawa, Ontario, Canada.
authors.forename: Mark W
authors.initials: MW
authors.lastname: Sumarah
authors.forename: Eva
authors.initials: E
authors.lastname: Puniani
authors.forename: Dan
authors.initials: D
authors.lastname: Sørensen
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Blackwell
authors.forename: J David
authors.initials: JD
authors.lastname: Miller
mesh_terms: D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D005658:Fungi / Q000378:metabolism*; D007306:Insecticides; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D028222:Picea / Q000382:microbiology*
chemical_list: D007306:Insecticides