High GC contents of primer 5'-end increases reaction efficiency in polymerase chain reaction.
Many studies have suggested that regulation of the polymerase chain reaction (PCR) is influenced by several factors. However, the understanding of reaction efficiency factors is not sufficient. Here we propose that high GC contents of primer 5'-end increases reaction efficiency in PCR. Using 71 primers (45 pairs), we analyzed factors that affect reaction efficiency, and statistically tested the correlation between the amplification signals and several factors. As a result, there were significant correlations between the amplification signals and the GC contents in the first 1 approximately 3 bps of primer 5'-end.
Shinoda
Naoki
N
Food and Agricultural Materials Inspection Center, Saitama, Japan. naoki_shinoda@nm.famic.go.jp
Yoshida
Tomotaro
T
Kusama
Toyoko
T
Takagi
Masami
M
Hayakawa
Toshiaki
T
Onodera
Takashi
T
Sugiura
Katsuaki
K
D000818:Animals; D017931:DNA Primers / Q000737:chemistry*; D004272:DNA, Mitochondrial / Q000737:chemistry; D020862:GC Rich Sequence; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004272:DNA, Mitochondrial