title: Consil bioactive glass particles enhance osteoblast proliferation and selectively modulate cell signaling pathways in vitro.
abstract: Consil Bioglass is a commercially available bioactive glass formulation previously shown in clinical studies to support osteogenesis and the repair of bony defects in dogs and cats. Previous in vitro studies confirm that Consil particles are able to bond directly with bone while promoting osteoblast proliferation and extracellular matrix production. However, the cellular mechanisms mediating their clinical effect remain unclear. This study evaluated whether enhancement of osteoblast proliferation by Consil particles is associated with signal transduction. Consil particles maintained the osteoblast phenotype and enhanced proliferation of canine osteoblasts for up to 21 days in culture. Stimulation of proliferation and maintenance of phenotype expression were accompanied by the modulation of selective cell signaling pathways including integrins, the mitogen-activated protein kinases (MAPKs), and the immediate-early gene c-Jun. These genes have been documented to mediate osteoblast growth and differentiation. The signal transduction occurs in a time-dependent manner in which Consil particles induce a decrease in the pattern of MAPK and c-Jun gene transcription from 4 to 24 h and a subsequent return to control levels by 7 days in culture. Our observations suggest that Consil Bioglass particles may provide cues that enhance cell division necessary for facilitating bone regeneration and the repair of bony defects.
authors.affiliation: Nutramax Laboratories, Inc., 2208 Lakeside Boulevard, Edgewood, Maryland 21040, USA.
authors.forename: Angela Y
authors.initials: AY
authors.lastname: Au
authors.forename: Rebecca Y
authors.initials: RY
authors.lastname: Au
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Demko
authors.forename: Ron M
authors.initials: RM
authors.lastname: McLaughlin
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Eves
authors.forename: Carmelita G
authors.initials: CG
authors.lastname: Frondoza
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001861:Bone Regeneration / Q000502:physiology; D002415:Cats; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D002516:Ceramics / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D004285:Dogs; D015870:Gene Expression; D006801:Humans; D039564:Integrin alphaV / Q000235:genetics / Q000378:metabolism; D008422:Materials Testing; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D010641:Phenotype; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; C022107:Bioglass; D039564:Integrin alphaV; D004247:DNA; D020928:Mitogen-Activated Protein Kinases