A simple and efficient method for extraction of PCR-amplifiable DNA from chicken eggshells.
Recently, we reported a method for discriminating a Japanese brand of chicken, the Hinai-jidori. As an application of this method for discriminating Hinai-jidori eggs, we here report an efficient method for extracting maternal DNA from eggshells. Eggshell powder was completely decalcified with EDTA solution, and then DNA was isolated by conventional phenol-chloroform extraction and ethanol precipitation. The efficiency of DNA recovery from eggshells was 50-fold higher than that of a previously reported method. The recovered DNA could be used for PCR, and 10 markers for identifying the Hinai-jidori chicken were detected. The genotypes of the Hinai-jidori exactly matched those of the Hinai-dori breed. Using this method, Hinai-jidori and Hinai-dori eggs could be distinguished from the eggs of Rhode Island Reds. This is the first report of a technique that can be used to discriminate the eggs of Hinai-jidori from those of other chickens, and it can also be utilized to validate the labeling of Hinai-jidori eggs in the market.
Rikimaru
Kazuhiro
K
Livestock Experiment Station, Akita Prefectural Agriculture, Forestry and Fisheries Research Center, Daisen, Japan.
Takahashi
Hideaki
H
D000822:Animal Husbandry; D000818:Animals; D002645:Chickens; D004247:DNA / Q000302:isolation & purification*; D004527:Egg Proteins / Q000737:chemistry* / Q000235:genetics; D004528:Egg Shell / Q000378:metabolism*; D005838:Genotype; D016133:Polymerase Chain Reaction
D004527:Egg Proteins; D004247:DNA