Follistatin-like-1, a diffusible mesenchymal factor determines the fate of epithelium.
Mesenchyme is generally believed to play critical roles in "secondary induction" during organogenesis. Because of the complexity of tissue interactions in secondary inductions, however, little is known about the precise mechanisms at the cellular and molecular levels. We have demonstrated that, in mouse oviductal development, the mesenchyme determines the fate of undetermined epithelial cells to become secretory or cilial cells. We have established a model for studying secondary induction by establishing clonal epithelial and mesenchymal cell lines from perinatal p53(-/-) mouse oviducts. The signal sequence trap method collected candidate molecules secreted from mesenchymal cell lines. Naive epithelial cells exposed to Follistatin-like-1 (Fstl1), one of the candidates, became irreversibly committed to expressing a cilial epithelial marker and differentiated into ciliated cells. We concluded that Fstl1 is one of the mesenchymal factors determining oviductal epithelial cell fate. This is a unique demonstration that the determination of epithelial cell fate is induced by a single diffusible factor.
Umezu
Tomohiro
T
Department of Biological Science and Technology, and Tissue Engineering Research Center, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
Yamanouchi
Hiromi
H
Iida
Yusuke
Y
Miura
Masataka
M
Tomooka
Yasuhiro
Y
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005187:Fallopian Tubes / Q000166:cytology; D005260:Female; D038702:Follistatin-Related Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D053538:Keratin-18 / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D041681:NIH 3T3 Cells; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
C481963:FOXJ1 protein, mouse; D038702:Follistatin-Related Proteins; D051858:Forkhead Transcription Factors; D006023:Glycoproteins; D053538:Keratin-18; C097024:Ovgp1 protein, mouse