title: 1-Ethyl-3-methylimidazolium ethylsulfate/copper catalyst for the enhancement of glucose chemiluminescent detection: effects on light emission and enzyme activity.
abstract: The effect of the ionic liquid 1-ethyl-3-methylimidazolium ethylsulfate ([Emim][EtSO(4)]) on the copper-catalyzed luminol chemiluminescence (CL) is reported. A drastic light emission enhancement is observed, related to a strong interaction between Cu(2+) and the imidazolium ring. In these conditions, the CL reaction was able to produce light efficiently at pH as low as 6.5 (amplification factor: Intensity(+IL)/Intensity(-IL) = 2900). Interesting effects of [Emim][EtSO(4)] on the enzyme glucose oxidase activity were also evidenced, and advantages were taken from this enhancement to perform sensitive chemiluminescent glucose detection (LOD = 4 microM) at pH 8.0.
authors.affiliation: Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Lyon 1-CNRS 5246 ICBMS, Bâtiment CPE 43, bd du 11 novembre 1918, 69622 Villeurbanne Cedex, France.
authors.forename: Aurélie A-M
authors.initials: AA
authors.lastname: Santafé
authors.forename: Bastien
authors.initials: B
authors.lastname: Doumèche
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
authors.forename: Agnès P
authors.initials: AP
authors.lastname: Girard-Egrot
authors.forename: Christophe A
authors.initials: CA
authors.lastname: Marquette
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D002384:Catalysis; D003300:Copper / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis; D007093:Imidazoles / Q000737:chemistry*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*
chemical_list: D007093:Imidazoles; D003300:Copper; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; C518739:1-ethyl-3-methylimidazolium