title: Molecular characterization, induced expression, and transcriptional regulation of porcine S100A12 gene.
abstract: The S100A12 gene belongs to the S100 family of genes, which are specific to vertebrates. It is involved in many inflammatory diseases of human and has been considered as a powerful diagnostic gene. In the present study, we identified the porcine S100A12 (pS100A12) gene, provided evidence that pS100A12 is located on chromosome 4 and is closely linked to SW512. We show that pS100A12 is expressed preferentially in immune organs/tissues, e.g., bone marrow, spleen, and inguinal lymph nodes. Expression of the pS100A12 gene is dramatically induced in porcine whole blood cultures by both lipopolysaccharide (LPS) and polyinosine-polycytidylic acid (Poly(I:C)). Elevated expression of pS100A12 is also correlated with in vivo infection with porcine circovirus type 2 (PCV-2) from at least 48h post infection. By analyzing a series of pS100A12 promoter reporter constructs, we have defined two crucial regions (-1013 to -590, -135 to -50) that are responsible for LPS- and Poly(I:C)-induced transcriptional activation, and demonstrated that the LPS/Poly(I:C)-PKC-C/EBPb-pS100A12 pathway may play a critical role in the transcription of the pS100A12.
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
authors.forename: Songbai
authors.initials: S
authors.lastname: Yang
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Juan
authors.initials: J
authors.lastname: Tang
authors.forename: Xinyun
authors.initials: X
authors.lastname: Li
authors.forename: Qigai
authors.initials: Q
authors.lastname: He
authors.forename: Shuhong
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D016678:Genome; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011070:Poly I-C / Q000276:immunology; D011401:Promoter Regions, Genetic; D009418:S100 Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D016415:Sequence Alignment; D013552:Swine / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic
chemical_list: D008070:Lipopolysaccharides; D009418:S100 Proteins; D011070:Poly I-C