Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds.
Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. The chemical gene switch activated gene expression under in vitro conditions in somatic cotyledonary embryos and zygotic seed embryos cultured from transgenic soybean plants, as well as in seeds in planta under greenhouse conditions. The efficiency of induction of GFP expression under different growth conditions was strongly influenced by the developmental stage of the seed and availability of the inducer. The formation of ER-derived GFP-containing protein bodies in seed storage parenchyma cells was correlated with the level of induced expression.
Semenyuk
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EG
Donald Danforth Plant Science Center, 975 N. Warson Road, Saint Louis, MO 63132, USA. centaurea@mail.ru
Schmidt
M A
MA
Beachy
R N
RN
Moravec
T
T
Woodford-Thomas
T
T
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004440:Ecdysone / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006834:Hydrazines / Q000494:pharmacology; D007605:Juvenile Hormones / Q000494:pharmacology; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics; D013025:Glycine max / Q000187:drug effects / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
D017931:DNA Primers; D006834:Hydrazines; D007605:Juvenile Hormones; D011994:Recombinant Proteins; D015534:Trans-Activators; D049452:Green Fluorescent Proteins; D004440:Ecdysone; C406712:methoxyfenozide