Inhibition of oxidative stress-induced amyloid beta formation in NT2 neurons by culture filtrate of a strain of Streptomyces antibioticus.
Actinomycetes isolated from Iran soil habitats were tested for the capacity to produce compounds which can protect neurons from cell death generated by oxidative stress in NT2 neurons. Confirmation of our initial hit was accomplished via the determination of amyloid beta level using the enzyme-linked immunosorbent assay test. The most interesting amyloid beta formation inhibitor discovered in our study was a secondary metabolite which was produced by strain HM45. This bioactive strain was identified as a strain of Streptomyces antibioticus DSM 40234 using polyphasic approach. The strain HM45 was deposited in Deutsche Sammlung von Mikroorganismen und Zellkulturen as S. antibioticus DSM 41955 and University of Tehran Microorganisms Sollection as S. antibioticus UTMC 00105. This work is the first report on efficiency of an actinomycete metabolite in prohibition of neurons death caused by amyloid beta formation.
Eftekharzadeh
Bahareh
B
Neuroscience Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran.
Hamedi
Javad
J
Mohammadipanah
Fatemeh
F
Khodagholi
Fariba
F
Maghsoudi
Nader
N
Klenk
Hans Peter
HP
D016229:Amyloid beta-Peptides / Q000378:metabolism*; D016923:Cell Death; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005798:Genes, Bacterial; D020459:Genes, rRNA; D006801:Humans; D007492:Iran; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D018384:Oxidative Stress; D010446:Peptide Fragments / Q000378:metabolism*; D010802:Phylogeny; D012988:Soil Microbiology; D013303:Streptomyces antibioticus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)