Retinoic acid-inducible gene-I (RIG-I) is induced by IFN-{gamma} in human mesangial cells in culture: possible involvement of RIG-I in the inflammation in lupus nephritis.
Interferon-gamma is a potent Th1-type cytokine and a key molecule in the pathogenesis of autoimmune diseases including lupus nephritis. Retinoic acid-inducible gene-I is a putative RNA helicase that plays an important role in immune and inflammatory reactions. We previously demonstrated the increased expression of the retinoic acid-inducible gene-I protein in the kidney tissue of patients with lupus nephritis, and the presence of a significant amount of retinoic acid-inducible gene-I mRNA in the urinary sediment of patients with this inflammatory renal disease. In the present study, interferon-gamma was found to induce the expression of retinoic acid-inducible gene-I in human mesangial cells in culture. Knockdown of retinoic acid-inducible gene-I inhibited the interferon-gamma-induced upregulation of interferon regulatory factor 7, a transcriptional factor involved in immune and inflammatory reactions. These findings suggest that retinoic acid-inducible gene-I produced by mesangial cells may be involved in the pathogenesis of lupus nephritis.
Imaizumi
T
T
Department of Vascular Biology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
Tanaka
H
H
Tajima
A
A
Tsuruga
K
K
Oki
E
E
Sashinami
H
H
Matsumiya
T
T
Yoshida
H
H
Inoue
I
I
Ito
E
E
D002478:Cells, Cultured; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000503:physiopathology; D050839:Interferon Regulatory Factor-7 / Q000235:genetics; D007371:Interferon-gamma / Q000378:metabolism*; D008181:Lupus Nephritis / Q000235:genetics / Q000503:physiopathology; D050527:Mesangial Cells / Q000378:metabolism*; D011971:Receptors, Immunologic; D015854:Up-Regulation
D050839:Interferon Regulatory Factor-7; D011971:Receptors, Immunologic; D007371:Interferon-gamma; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases