Antioxidant status and lipid peroxidation activity in evaluating hepatocellular damage in children.
Serum levels of glutathione reductase (GR), glutathione S-transferase-alpha (GST-alpha) and malondialdehyde (MDA) were determined to evaluate their use in diagnosing hepatocellular damage in 75 children with liver disease. Except for level of GR in patients with HBV, GR, GST-alpha and MDA were raised significantly in patients compared with controls. At 100% specificity, the sensitivity of the 3 markers for detecting hepatitis B virus, hepatitis C virus and schistosomiasis infection respectively were: 16.7%, 100.0% and 17.7% for GR; 33.3%, 62.1% and 38.2% for GST-alpha; and 25.0%, 10.3% and 29.4% for MDA. GR was more sensitive in hepatitis C infection, while MDA reflected changes in liver ultrasound and GST-alpha was the best indicator for histopathological changes.
Mahdy
K A
KA
Department of Medical Biochemistry, Medical Research Division, National Research Centre, Cairo, Egypt. Karammahdy@yahoo.com
Abd-El-Shaheed
A
A
Khadr
M E
ME
El-Shamy
K A I
KA
D000293:Adolescent; D000704:Analysis of Variance; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004534:Egypt; D005260:Female; D005980:Glutathione Reductase / Q000097:blood*; D005982:Glutathione Transferase / Q000097:blood*; D006509:Hepatitis B / Q000150:complications; D006526:Hepatitis C / Q000150:complications; D006801:Humans; D015227:Lipid Peroxidation; D008107:Liver Diseases / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism; D008111:Liver Function Tests; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D012552:Schistosomiasis / Q000150:complications; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index
D000975:Antioxidants; D015415:Biomarkers; D008315:Malondialdehyde; D005980:Glutathione Reductase; D005982:Glutathione Transferase