title: Encephalitogenic T-cells increase numbers of CNS T-cells regardless of antigen specificity by both increasing T-cell entry and preventing egress.
abstract: This study utilized an adoptive transfer model of experimental autoimmune encephalomyelitis (EAE) induction in mice to characterize the mechanisms involved in CNS accumulation of transferred and host T-cells. Using a flow cytometric technique, we examined phenotypic characteristics of CNS T-cells following disease initiation and the role of T-cell activation in CNS invasion and retention. Host T-cell activation increased cell recruitment and EAE severity. CNS antigen specific T-cells were required to induce T-cell retention within the CNS. Once retention was initiated, CNS T-cells were retained regardless of specificity. This study characterizes mechanisms involved in CNS accumulation of T-cells during EAE pathogenesis.
authors.affiliation: Department of Surgery, 400 MSTF, 10 S. Pine St, University of Maryland School of Medicine, Baltimore 21201-1509, USA. jason.lees@gmail.com
authors.forename: Jason R
authors.initials: JR
authors.lastname: Lees
authors.forename: Julia
authors.initials: J
authors.lastname: Sim
authors.forename: John H
authors.initials: JH
authors.lastname: Russell
mesh_terms: D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D002452:Cell Count; D002460:Cell Line; D002465:Cell Movement / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D002490:Central Nervous System / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000503:physiopathology; D000939:Epitopes / Q000276:immunology*; D006790:Host-Parasite Interactions / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D000939:Epitopes