title: Carbohydrate binding specificities and crystal structure of the cholera toxin-like B-subunit from Citrobacter freundii.
abstract: Enterotoxigenic Escherichia coli and Vibrio cholerae are well known causative agents of severe diarrheal diseases. Both pathogens produce AB(5) toxins, with one enzymatically active A-subunit and a pentamer of receptor-binding B-subunits. The primary receptor for both B-subunits is the GM1 ganglioside (Galbeta3GalNAcbeta4(NeuAcalpha3)Galbeta4GlcbetaCer), but the B-subunits from porcine isolates of E. coli also bind neolacto-(Galbeta4GlcNAcbeta-)terminated glycoconjugates and the B-subunits from human isolates of E. coli (hLTB) have affinity for blood group A type 2-(GalNAcalpha3(Fucalpha2)Galbeta4GlcNAcbeta-)terminated glycoconjugates. A B-subunit with 73% sequence identity to the B-subunits of cholera toxin and the heat-labile toxin of E. coli is produced by certain strains of enteropathogenic E. coli and by Citrobacter freundii. This C. freundii B-subunit (CFXB) has now been expressed in V. cholerae, and isolated in high yields. Glycosphingolipid binding studies show that CFXB binds to the GM1 ganglioside with high affinity. In addition, CFXB has high affinity for both neolacto-terminated and blood group A type 2-terminated glycoconjugates. The crystal structure of the pentameric arrangement of C. freundii B-subunits display high structural similarity with related proteins from E. coli and V. cholerae and oligosaccharide binding sites can be identified on the protein surface. Small changes in the 88-95 loop connecting the GM1 and blood group A binding sites explains the minor changes in affinity seen for these two ligands. However, the enhanced affinity of CFXB for neolacto-terminated structures can be sought in the Lys34Tyr substitution affording additional hydrogen bond interactions between the tyrosyl side chain and the GlcNAcbeta3Galb4Glcbeta1 segment of neolactotetraosylceramide via bridging water molecules.
authors.affiliation: Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, University of Gothenburg, PO Box 440, SE-40530 Göteborg, Sweden.
authors.forename: Lena
authors.initials: L
authors.lastname: Jansson
authors.forename: Jonas
authors.initials: J
authors.lastname: Angström
authors.forename: Michael
authors.initials: M
authors.lastname: Lebens
authors.forename: Anne
authors.initials: A
authors.lastname: Imberty
authors.forename: Annabelle
authors.initials: A
authors.lastname: Varrot
authors.forename: Susann
authors.initials: S
authors.lastname: Teneberg
mesh_terms: D000595:Amino Acid Sequence; D002151:Calorimetry; D050260:Carbohydrate Metabolism; D002772:Cholera Toxin / Q000737:chemistry / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D016971:Citrobacter freundii / Q000378:metabolism*; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D002772:Cholera Toxin