Cytotoxic activity of flavonoids from the flowers of Chrysanthemum morifolium on human colon cancer Colon205 cells.
A new p-hydroxyphenylacetyl flavonoid, diosmetin 7-(6''-O-p-hydroxyphenylacetyl)-O-beta-d-glucopyranoside (1), was isolated from the flowers of Chrysanthemum morifolium Ramat. 'huaiju' cv. nov. (Compositae), together with five known flavonoids, luteolin (2), diosmetin (3), diosmetin 7-O-beta-d-glucopyranoside (4), diosmin (5), and scolimoside (6), and four known caffeoylquinic acid derivatives, macranthoin F (7), 3,5-dicaffeoylquinic acid (8), 1,3-dicaffeoyl-epi-quinic acid (9), and chlorogenic acid (10). The structure of 1 was elucidated by UV, IR, ESI-TOF-MS, 1D, and 2D NMR spectroscopic methods. Cytotoxic activity of compounds 1-5 against human colon cancer cell Colon205 was investigated using MTT assays. Compounds 2 and 3 showed significant cytotoxicities against Colon205, with their IC(50) values being 96.9 and 82.9 microM, respectively. However, compounds 1, 4, and 5 showed little cytotoxic activity.
Xie
Yuan-Yuan
YY
Department of Traditional Chinese Medicines, Shenyang Pharmaceutical University, China.
Yuan
Dan
D
Yang
Jing-Yu
JY
Wang
Li-Hui
LH
Wu
Chun-Fu
CF
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D031202:Chrysanthemum / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D005419:Flavonoids