title: Effect of the number of biopsies on the subsequent acquisition of new human papillomavirus infections.
abstract: OBJECTIVE
  To analyze the effect of number of biopsies taken at enrollment with incident human papillomavirus (HPV) infections detected at the next 6-month visit.
  
  
  METHODS
  Using data from the Atypical Squamous Cells of Undetermined Significance (ASCUS) and Low-Grade Squamous Intraepithelial Lesion (LSIL) Triage Study (ALTS), we compared the 6-month acquisition of new HPV infections among 988 women who underwent colposcopy, were not diagnosed and treated for cervical intraepithelial neoplasia grade 2 or more severe lesions, and had polymerase chain reaction results for 38 HPV genotypes at enrollment and follow-up. Our analysis considered each woman's possible acquisition of each of these HPV genotypes.
  
  
  RESULTS
  The average 6-month acquisition of any HPV genotype for women with zero, one, and two or more biopsies was 1.82%, 1.74%, and 1.97%, respectively (Ptrend=.7). In a logistic regression model that controlled for age, baseline HPV status, and having a new sexual partner during the 6-month follow-up, two or more biopsies (compared with one biopsy) was not associated with acquiring HPV (odds ratio 1.0, 95% confidence interval 0.75-1.3).
  
  
  CONCLUSIONS
  Multiple biopsies compared with a single biopsy did not increase the likelihood of acquiring new HPV infections.
  
  
  METHODS
  II.
authors.affiliation: From the Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland; Departments of Molecular Genetics and Microbiology and Obstetrics and Gynecology, University of New Mexico Health Sciences Center, School of Medicine, Albuquerque, New Mexico; and Information Management Services, Inc., Silver Spring, Maryland.
authors.forename: Philip E
authors.initials: PE
authors.lastname: Castle
authors.forename: Nicolas
authors.initials: N
authors.lastname: Wentzensen
authors.forename: Cosette M
authors.initials: CM
authors.lastname: Wheeler
authors.forename: Greg
authors.initials: G
authors.lastname: Rydzak
authors.forename: Mark
authors.initials: M
authors.lastname: Schiffman
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy / Q000009:adverse effects*; D003127:Colposcopy; D005260:Female; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000635:transmission*; D013997:Time Factors; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis