title: Production, refining, structural characterization and fermentability of rice husk xylooligosaccharides.
abstract: Oligosaccharides produced by hydrothermal processing of rice husks (xylooligosaccharides and glucooligosaccharides) were refined by membrane processing (operating in diafiltration and concentration modes), subjected to xylanase treatment to reduce the average molar mass, and subjected to further purification by ultrafiltration (operating in concentration mode) and ion exchange. The purified products were assayed for composition, molar mass distribution and structural characterization by HPLC, HPAEC-PAD, HPSEC, MALDI-TOF-MS and NMR ((1)H and (13)C). The fermentability of the purified product by fecal inocula was assessed on the basis of the time courses of pH and oligosaccharide concentrations. Succinate, lactate, formiate, acetate, propionate and butyrate were the major products resulting from fermentation experiments.
authors.affiliation: Department of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas, Ourense, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Gullón
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: González-Muñoz
authors.forename: Martine Paula
authors.initials: MP
authors.lastname: van Gool
authors.forename: Henk Arie
authors.initials: HA
authors.lastname: Schols
authors.forename: Ján
authors.initials: J
authors.lastname: Hirsch
authors.forename: Anna
authors.initials: A
authors.lastname: Ebringerová
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Parajó
mesh_terms: D005285:Fermentation; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D012275:Oryza / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009844:Oligosaccharides