title: Novel SNP in 5' flanking region of EDG1 associated with marbling in Japanese Black beef cattle.
abstract: Marbling, defined by the amount and distribution of intramuscular fat, is an economically important trait of beef cattle in Japan. The endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 (EDG1) gene has been considered as a positional functional candidate for the gene responsible for marbling. We have recently reported that 2 single nucleotide polymorphisms (SNPs), c.-312A>G in the 5' untranslated region (UTR) and c.*446G>A in the 3' UTR in EDG1 were associated with marbling in Japanese Black beef cattle, but this was not functional and a causal mutation for marbling. In the present study, we detected 2 novel SNPs, referred to as g.1475435G>A and g.1471620G>T, in the 5' flanking region of the EDG1 between low-marbled and high-marbled steer groups, which were previously shown to have EDG1 expression differences in musculus longissimus muscle. The g.1475435G>A SNP seemed not to segregate in Japanese Black beef cattle. The g.1471620G>T SNP was associated with the predicted breeding value for beef marbling standard number by the analyses using Japanese Black beef cattle population. Based on these findings, we hypothesized that the g.1471620G>T SNP might have an impact on EDG1 expression and also marbling.
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, Japan. tyamada@kais.kyoto-u.ac.jp
authors.forename: Takahisa
authors.initials: T
authors.lastname: Yamada
authors.forename: Seiki
authors.initials: S
authors.lastname: Sasaki
authors.forename: Shin
authors.initials: S
authors.lastname: Sukegawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miyake
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Fujita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kose
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Morita
authors.forename: Youichi
authors.initials: Y
authors.lastname: Takahagi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murakami
authors.forename: Fumiki
authors.initials: F
authors.lastname: Morimatsu
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sasaki
mesh_terms: D024506:5' Flanking Region; D000818:Animals; D002417:Cattle; D008297:Male; D008460:Meat / Q000592:standards*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*