Nanopillar, nanoball, and nanofibers for highly efficient analysis of biomolecules.
DNA separation technologies combined with micro- and nanofabrication technologies found a breakthrough in genotyping and DNA sequencing. This tutorial review outlines the fabrication technologies for nano-scaled structures inside microchannels and how the precisely designed structures contribute to obtaining higher performances in DNA separations from the viewpoint of the fabrication process, "top-down" nanofabrication and "bottom-up" molecular self-assembly approaches. It was found that these nanofabricated structures generated the unique separation modes that could not be achieved by random-sized pores of conventional gel or polymer systems. Furthermore, it was found that nanoscale-specific phenomena such as electroosmotic flow should be taken into consideration for further development of nanofabricated structures in DNA analysis. These separation technologies will contribute as a core technology for a future integrated biomolecule anlaysis chip.
Kaji
Noritada
N
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan. kaji@apchem.nagoya-u.ac.jp
Okamoto
Yukihiro
Y
Tokeshi
Manabu
M
Baba
Yoshinobu
Y
D015374:Biosensing Techniques / Q000295:instrumentation; D004247:DNA / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D017422:Sequence Analysis, DNA; D013997:Time Factors
D004247:DNA