title: Hierarchical organization of the plasma membrane: investigations by single-molecule tracking vs. fluorescence correlation spectroscopy.
abstract: Single-molecule tracking and fluorescence correlation spectroscopy (FCS) applied to the plasma membrane in living cells have allowed a number of unprecedented observations, thus fostering a new basic understanding of molecular diffusion, interaction, and signal transduction in the plasma membrane. It is becoming clear that the plasma membrane is a heterogeneous entity, containing diverse structures on nano-meso-scales (2-200 nm) with a variety of lifetimes, where certain membrane molecules stay together for limited durations. Molecular interactions occur in the time-dependent inhomogeneous two-dimensional liquid of the plasma membrane, which might be a key for plasma membrane functions.
authors.affiliation: Membrane Mechanisms Project, International Cooperative Research Project (ICORP), Japan Science and Technology Agency (JST), Center for Meso-Bio Single-Molecule Imaging (CeMI), Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kusumi
authors.forename: Yuki M
authors.initials: YM
authors.lastname: Shirai
authors.forename: Ikuko
authors.initials: I
authors.lastname: Koyama-Honda
authors.forename: Kenichi G N
authors.initials: KG
authors.lastname: Suzuki
authors.forename: Takahiro K
authors.initials: TK
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D004058:Diffusion; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D008958:Models, Molecular; D055503:Protein Multimerization / Q000502:physiology; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D008563:Membrane Lipids; D008565:Membrane Proteins