title: Re-visiting Luck's classification: a histological analysis of Dupuytren's disease.
abstract: Luck (1959) described a histological staging system for Dupuytren's disease, classifying the disease into three stages. Previous biochemical and immunochemical studies have detailed the decrease in type III/I collagen ratio with disease progression. Herovici (1963) described a histological stain that produced a differential red/purple and blue colour for type I and III collagen respectively. We stained 15 specimens of Dupuytren's disease and quantified the different collagen types in each using computer analysis. We found a corresponding decrease in the amount of type III collagen as a percentage of the total collagen with disease progression: stage I range 35-49% (mean 38%); stage 2 range 21-33% (mean 27%) and stage 3 range 11-19% (mean 14%). We propose a new staging system based on the relative amount of type III collagen, where stage 1: >35%, stage 2: >20% and <35%, and stage 3: <20%.
authors.affiliation: Department of Plastic Surgery and Burns Research Unit, School of Biomedical Sciences, University of Bradford, Bradford, United Kingdom. lamweeleon@gmail.com
authors.forename: W L
authors.initials: WL
authors.lastname: Lam
authors.forename: J M
authors.initials: JM
authors.lastname: Rawlins
authors.forename: R O S
authors.initials: RO
authors.lastname: Karoo
authors.forename: I
authors.initials: I
authors.lastname: Naylor
authors.forename: D T
authors.initials: DT
authors.lastname: Sharpe
mesh_terms: D000368:Aged; D049109:Cell Proliferation; D015331:Cohort Studies; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D004396:Coloring Agents; D004387:Dupuytren Contracture / Q000145:classification* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D009930:Organic Chemicals; D012720:Severity of Illness Index; D013194:Staining and Labeling
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D004396:Coloring Agents; C104366:Herovici's stain; D009930:Organic Chemicals