title: Overexpression of Chromatin Assembly Factor-1/p60 helps to predict the prognosis of melanoma patients.
abstract: BACKGROUND
  Cutaneous melanoma (CM) is the most lethal form of skin malignancy, which registers a constant increase in incidence worldwide. The identification of molecular alteration(s) involved in its biological aggressiveness represents a major challenge for researchers, considering that existing therapies are ineffective to treat metastasizing cases. The epigenetic control of chromatin dynamics during DNA synthesis, replication, and repair is fundamental for the orderly progression of cell proliferation. The Chromatin Assembly Factor 1 (CAF-1) complex acts as a major regulator of this process; its intermediate (p60) subunit has been recently proposed as a novel proliferation and prognostic marker for several tumors. We aimed to establish if the evaluation of the expression of CAF-1/p60 in primary CM may help define the prevision of outcome of patients.
  
  
  METHODS
  Immunohistochemistry with anti-CAF-1/p60 was performed on paraffin-embedded tissue sections of 130 cases of primary CM retrieved from the archive files of the Department of Biomorphological and Functional Sciences, Section of Pathology, University "Federico II" of Naples, Italy. Results were compared with histopathological and follow-up data of patients.
  
  
  RESULTS
  CAF-1/p60 was expressed in all CM. A significant statistical association between the overexpression of the protein and the occurrence of skin, node and/or distant metastases (P < 0.05) emerged, independently from histopathological prognostic factors.
  
  
  CONCLUSIONS
  CAF-1/p60 looks promising as a new prognostic marker for CM and sheds new light on the molecular events associated with photocancerogenesis and melanoma biology.The screening for CAF-1/p60 might contribute to the molecular sub-classification of CM, with improved translational outcomes.
authors.affiliation: Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples Federico II, School of Medicine, Naples, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Mascolo
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Vecchione
authors.forename: Gennaro
authors.initials: G
authors.lastname: Ilardi
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Scalvenzi
authors.forename: Guido
authors.initials: G
authors.lastname: Molea
authors.forename: Maria
authors.initials: M
authors.lastname: Di Benedetto
authors.forename: Loredana
authors.initials: L
authors.lastname: Nugnes
authors.forename: Maria
authors.initials: M
authors.lastname: Siano
authors.forename: Gaetano
authors.initials: G
authors.lastname: De Rosa
authors.forename: Stefania
authors.initials: S
authors.lastname: Staibano
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D056504:Chromatin Assembly Factor-1 / Q000096:biosynthesis*; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008545:Melanoma / Q000473:pathology*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011379:Prognosis; D012878:Skin Neoplasms / Q000473:pathology*; D016896:Treatment Outcome
chemical_list: D056504:Chromatin Assembly Factor-1