Epitope-cavities generated by molecularly imprinted films measure the coincident response to anthrax protective antigen and its segments.
A molecularly imprinted film was fabricated, in the presence of epitope-peptides, onto a quartz crystal microbalance (QCM) chip. These five peptides are known linear or conformational epitopes of the anthrax protective antigen PA(83). Imprinting resulted in an epitope-cavity with affinity for the corresponding template. With the use of a basic monomer, the binding-effect was further enhanced increasing the affinity to nanomolar levels. The affinities of the peptide to their corresponding molecularly induced polymers (MIPs) were more closely related to the molecular weight of the analyte than to the number of residues. All epitope-cavities differentiated their epitope region on the protective antigen PA(83) as well as the corresponding furin cleavage fragments PA(63) and PA(20). The QCM chip differential response to the protective antigen fragment was observed in the picomolar range, thus demonstrating a method to manipulate protein on the surface with defined orientation.
Tai
Dar-Fu
DF
Department of Chemistry, National Dong-Hwa University, Hualien, Taiwan. dftai@mail.ndhu.edu.tw
Jhang
Ming-Hong
MH
Chen
Guan-Yu
GY
Wang
Sue-Chen
SC
Lu
Kuo-Hao
KH
Lee
Yu-Der
YD
Liu
Hsin-Tzu
HT
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001427:Bacterial Toxins / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D054802:Molecular Imprinting; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D011791:Quartz / Q000737:chemistry; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D000939:Epitopes; D010455:Peptides; C030325:anthrax toxin; D011791:Quartz