[Red clover isoflavones inhibit the proliferation and promote the apoptosis of benign prostatic hyperplasia stromal cells].
OBJECTIVE
To evaluate the effects of red clover isoflavones on the proliferation and apoptosis of human benign prostatic hyperplasia (BPH) stromal cells.


METHODS
We treated human prostate stromal cells with red clover isoflavones at the concentration of 12.5, 25, 50 and 100 microg/ml, and established a PBS blank control, a dimethyl sulphoxide (DMSO) negative control and four finasteride positive control groups (at the concentration of 12.5, 25.0, 50.0 and 100.0 microg/ml). We determined the effects of different concentrations of red clover isoflavones on the proliferation of the cells by MTT assay and on their apoptosis by Annexin V/PI double staining flow cytometry.


RESULTS
Red clover isoflavones inhibited the proliferation of the BPH stromal cells by 18.86% at 25.0 microg/ml, compared with 5.17% in the blank control group (P < 0.05), and more obviously at a higher concentration. At 50.0 microg/ml, red clover isoflavones exhibited a weaker inhibitory effect than finasteride (28% vs 69.88% , P < 0.05). Annexin V/PI double staining flow cytometry showed that red clover isoflavones at 25.0 microg/ml induced the apoptosis of the prostate stromal cells by (18.54 +/- 2.5)%, with significant differences from the negative control and blank control (P < 0.01).


CONCLUSIONS
Red clover isoflavones can inhibit the proliferation and promote the apoptosis of human BPH stromal cells.
Chen
Mei-Yuan
MY
Department of Urology, Tongji Hospital Affiliated to Tongli Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. meiyuan.chen@gmail.com
Yan
Shu-Chao
SC
Yin
Chun-Ping
CP
Ye
Lei
L
Zhang
Meng-Ke
MK
Yang
Jun
J
Liu
Ji-Hong
JH
D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011467:Prostate / Q000166:cytology / Q000187:drug effects; D011470:Prostatic Hyperplasia / Q000188:drug therapy / Q000473:pathology*; D017154:Stromal Cells / Q000187:drug effects; D029921:Trifolium / Q000737:chemistry
D007529:Isoflavones; D010936:Plant Extracts