title: A novel fluorogenic coumarin substrate for monitoring acid phosphatase activity at low pH environment.
abstract: This article described the synthesis and application of 6-chloro-8-fluoro-4-methylumbelliferone phosphate (CF-MUP) in analyzing acid phosphatase activity. Compared to the existing MUP, the new coumarin phosphate, CF-MUP, demonstrateed much higher sensitivity and was more robust for detecting the activity of acid phosphatase than the classic substrate 4-methylumbelliferone phosphate (MUP). The product of enzyme reaction, 6-chloro-8-fluoro-4-methylumbelliferone (CF-MU) possesses strong fluorescence at approximately 450 nm with low pKa (4.7), high fluorescence quantum yield and pH independence in the physiological pH range. This new fluorescence dye, CF-MU, is a convenient tool for assays with buffer pH between 4.5 and 8.
authors.affiliation: Department of Chemistry & Chemical Engineering, Baoji University of Arts & Science, Shaanxi, China.
authors.forename: Desuo
authors.initials: D
authors.lastname: Yang
authors.forename: Zongxiao
authors.initials: Z
authors.lastname: Li
authors.forename: Yubo
authors.initials: Y
authors.lastname: Allan Diwu
authors.forename: Hanzhuo
authors.initials: H
authors.lastname: Fu
authors.forename: Jinfang
authors.initials: J
authors.lastname: Liao
authors.forename: Chunmei
authors.initials: C
authors.lastname: Wei
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Diwu