title: Relationships between tropomyosin and myosin heavy chain isoforms in bovine skeletal muscle.
abstract: The composition of tropomyosin (TPM) and myosin heavy chain (MyHC) isoforms was analyzed in 10 physiologically different bovine muscles (masseter, diaphragm, tongue, semispinalis, pectoralis profundus, biceps femoris, psoas major, semimembranosus, longissimus thoracis and semitendinosus) to clarify the relationships between TPM and MyHC isoforms in different muscle fiber types. The content of TPM1 and TPM3 was different in muscles according to their function in muscle contraction, although the content of TPM2 was constantly about 50% of the total TPM in all muscles. The content of TPM1 was higher in semimembranosus, longissimus thoracis and semitendinosus, while that of TPM3 was higher in masseter and diaphragm. The high positive correlation between MyHC-slow content and TPM3 content (r = 0.92) suggested a coexpression of TPM3 and MyHC-slow isoforms in a muscle fiber. MyHC-slow and TPM3 were expressed at the same level in masseter and diaphragm, whereas there was more TPM3 than MyHC-slow in tongue and semispinalis, so it appears that the excess TPM3 in tongue and semispinalis is expressed with other MyHC isoforms. MyHC-2a was the only fast type isoform expressed in tongue and semispinalis. Therefore, the excess TPM3 was composed of myofibrils with MyHC-2a. The results suggested that a fiber expressing MyHC-2a would be regulated delicately by changing the TPM isoform types.
authors.affiliation: Graduate School of Life and Environmental Science, University of Tsukuba, Tsukuba, Ibaraki, Japan. mooe@affrc.go.jp
authors.forename: Mika
authors.initials: M
authors.lastname: Oe
authors.forename: Ikuyo
authors.initials: I
authors.lastname: Nakajima
authors.forename: Susumu
authors.initials: S
authors.lastname: Muroya
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shibata
authors.forename: Koich
authors.initials: K
authors.lastname: Chikuni
mesh_terms: D000818:Animals; D002417:Cattle; D009119:Muscle Contraction; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000235:genetics*; D009928:Organ Specificity; D020033:Protein Isoforms / Q000235:genetics; D014335:Tropomyosin / Q000235:genetics*
chemical_list: D020033:Protein Isoforms; D014335:Tropomyosin; D018995:Myosin Heavy Chains