title: Analysis of the expression of human tumor antigens in ovarian cancer tissues.
abstract: Biomarkers for early detection of cancer have great clinical diagnostic potential. Numerous reports have documented the generation of humoral immune responses that are triggered in response to changes in protein expression patterns in tumor tissues and these biomarkers are referred to as tumor associated antigens (TAAs). Using a high-throughput technology, we previously identified 65 proteins as diagnostically useful TAAs by profiling the humoral immune responses in ovarian cancer (OVCA) patients. Here we determined the expression status of some of those TAAs in tissues from OVCA patients. The protein expression patterns of 4 of those 65 antigens, namely NASP, RCAS1, Nijmegen breakage syndrome1 (NBS1) and eIF5A, along with p53 and Her2 (known molecular prognosticators) and two proteins that interact with NBS1, MRE11 and RAD50, were assessed by immunohistochemistry (IHC). NASP and RCAS1 proteins were more frequently expressed in ovarian cancer tissues than with normal ovarian tissue and serous cystadenomas and MRE11 was less frequently expressed. When evaluated simultaneously, only NASP and MRE11 remained statistically significant with sensitivity of 66% and specificity of 89%. None of these proteins' expression levels were prognostic for survival. Together, our results indicate that occurrence of humoral immune responses against some of these TAAs in OVCA patients is triggered by antigen protein overexpression.
authors.affiliation: Department of Pathology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Rouba
authors.initials: R
authors.lastname: Ali-Fehmi
authors.forename: Madhumita
authors.initials: M
authors.lastname: Chatterjee
authors.forename: Alexei
authors.initials: A
authors.lastname: Ionan
authors.forename: Nancy K
authors.initials: NK
authors.lastname: Levin
authors.forename: Haitham
authors.initials: H
authors.lastname: Arabi
authors.forename: Sudeshna
authors.initials: S
authors.lastname: Bandyopadhyay
authors.forename: Jay P
authors.initials: JP
authors.lastname: Shah
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Bryant
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Hewitt
authors.forename: Michael G
authors.initials: MG
authors.lastname: O'Rand
authors.forename: Oleg M
authors.initials: OM
authors.lastname: Alekseev
authors.forename: Robert
authors.initials: R
authors.lastname: Morris
authors.forename: Adnan
authors.initials: A
authors.lastname: Munkarah
authors.forename: Judith
authors.initials: J
authors.lastname: Abrams
authors.forename: Michael A
authors.initials: MA
authors.lastname: Tainsky
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000008:administration & dosage* / Q000096:biosynthesis; D001324:Autoantigens / Q000096:biosynthesis; D014408:Biomarkers, Tumor / Q000032:analysis*; D018293:Cystadenoma, Serous / Q000175:diagnosis / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D000076228:MRE11 Homologue Protein; D008875:Middle Aged; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000096:biosynthesis; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D046888:Tissue Array Analysis; D055815:Young Adult
chemical_list: D000951:Antigens, Neoplasm; D001324:Autoantigens; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C120762:EBAG9 protein, human; C121766:MRE11 protein, human; C489390:NASP protein, human; D009687:Nuclear Proteins; D000076228:MRE11 Homologue Protein