Rapid identification and characterization of antioxidants from Ligularia fischeri.
The objectives of this study were to investigate the radical-scavenging activity of Ligularia fischeri on oxidative damage by the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to rapidly identify the active components using the bioassay-linked fractionation method. The MeOH extract and fractions of CH(2)Cl(2), BuOH, and H(2)O from L. fischeri showed DPPH radical-scavenging effects in a dose-dependent manner (p < 0.01). In particular, the BuOH fraction had the most effective (p < 0.05) antioxidative capacity. The active constituents from the BuOH fraction of L. fischeri were rapidly isolated by bioassay-linked HPLC method and identified as hyperoside and 2''-acetylhyperoside with potent antioxidant effects against the DPPH radical, with IC(50) values of 1.31 and 7.09 microg/mL, respectively. They have not been reported from L. fischeri yet.
Piao
Xiang-Lan
XL
Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing, 100081, China.
Mi
Xiao-Yuan
XY
Tian
Yan-Ze
YZ
Wu
Qian
Q
Piao
Hui-Shan
HS
Zeng
Zhikai
Z
Wang
Ding
D
Piao
Xiangshu
X
D000975:Antioxidants / Q000737:chemistry*; D019659:Asteraceae / Q000737:chemistry*; D001681:Biological Assay; D001713:Biphenyl Compounds / Q000737:chemistry; D000440:Butanols / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000737:chemistry; D007202:Indicators and Reagents; D007723:Korea; D009682:Magnetic Resonance Spectroscopy; D010851:Picrates / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D000440:Butanols; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl