Cyclooxygenase-2 in testes of infertile men: evidence for the induction of prostaglandin synthesis by interleukin-1β.
As we previously reported, testes of men suffering from hypospermatogenesis and germ cell arrest or Sertoli cell-only syndrome show a major increase in the number of macrophages expressing interleukin-1β (IL-1β) and abundant expression of cyclooxygenase-2 (COX-2), the inducible isoform of the key enzyme in the biosynthesis of prostaglandins (PGs), in Leydig cells. In the present study we report [1] a positive correlation between IL-1β levels and COX-2 expression in testes of infertile patients, [2] the induction of COX-2 by IL-1β in mouse Leydig cells (TM3) and human macrophages (THP-1), and therefore [3] evidence for an IL-1β-dependent induction of testicular inflammatory states.
Matzkin
María Eugenia
ME
Laboratorio de Esteroides, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Buenos Aires, Argentina.
Mayerhofer
Artur
A
Rossi
Soledad Paola
SP
Gonzalez
Betina
B
Gonzalez
Candela Rocío
CR
Gonzalez-Calvar
Silvia Inés
SI
Terradas
Claudio
C
Ponzio
Roberto
R
Puigdomenech
Elisa
E
Levalle
Oscar
O
Calandra
Ricardo Saúl
RS
Frungieri
Mónica Beatriz
MB
D000328:Adult; D000818:Animals; D001706:Biopsy; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism* / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism* / Q000494:pharmacology; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D011453:Prostaglandins / Q000378:metabolism*; D054331:Sertoli Cell-Only Syndrome / Q000378:metabolism / Q000473:pathology; D013737:Testis / Q000378:metabolism* / Q000473:pathology
D053583:Interleukin-1beta; D011453:Prostaglandins; D051546:Cyclooxygenase 2