Development of liposome-based mimics of superoxide dismutase and peroxidase based on the "LIPOzyme" concept.
An antioxidative liposome catalyst, LIPOzyme, that mimics both superoxide dismutase (SOD) and peroxidase (POD)-like activities has been developed by using liposomes modified with simple ligands (dodecanoyl-histidine, Dodec-His) and metal ions (Mn). The SOD-like activity is dependent on the stability of the ligand-metal complex on the liposome membrane, with the value being higher for the DPPC liposome and at a higher pH. The POD-like activity was found to be maximal in the case of DMPC liposome, in which the ligand-metal complex is inserted more deeply into the membrane. It was thus shown that liposome modified with simple ligands can exhibit different enzyme-like activities depending on the characteristics of the liposome membrane.
Umakoshi
Hiroshi
H
Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.
Morimoto
Kengo
K
Yasuda
Naoto
N
Shimanouchi
Toshinori
T
Kuboi
Ryoichi
R
D040761:Biomimetic Materials / Q000138:chemical synthesis*; D001709:Biotechnology / Q000379:methods*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D044367:Phase Transition; D013482:Superoxide Dismutase / Q000378:metabolism*; D044366:Transition Temperature
D008081:Liposomes; D010544:Peroxidases; D013482:Superoxide Dismutase