title: Vezatin, a potential target for ADP-ribosylation factor 6, regulates the dendritic formation of hippocampal neurons.
abstract: ADP-ribosylation factor 6 (ARF6) is a small GTPase that regulates neuronal morphogenesis processes such as axonal, dendritic, and spine formation possibly through the actin cytoskeleton and membrane trafficking. In an attempt to define the molecular mechanisms that regulate neuronal morphogenesis by ARF6, we identified vezatin as a novel binding partner of active GTP-bound ARF6 using yeast two-hybrid screening. Vezatin was able to bind specifically to GTP-ARF6 among the ARF family. In the adult mouse brain, vezatin exhibited widespread gene expression with high levels in the hippocampus and medial habenular nucleus. In hippocampal neurons, vezatin was localized at dendrites as well as cell bodies. Knockdown of endogenous vezatin significantly reduced total dendritic length and arborization of cultured hippocampal neurons, while overexpression of vezatin increased dendritic length. Our present study suggests that vezatin may regulate dendritic formation as a downstream effector of ARF6.
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara 228-8555, Kanagawa, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Sanda
authors.forename: Naoki
authors.initials: N
authors.lastname: Ohara
authors.forename: Akifumi
authors.initials: A
authors.lastname: Kamata
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Hara
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tamaki
authors.forename: Jun
authors.initials: J
authors.lastname: Sukegawa
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Yanagisawa
authors.forename: Kohji
authors.initials: K
authors.lastname: Fukunaga
authors.forename: Hisatake
authors.initials: H
authors.lastname: Kondo
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakagami
mesh_terms: D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002490:Central Nervous System / Q000378:metabolism; D002522:Chlorocebus aethiops; D003712:Dendrites / Q000187:drug effects* / Q000502:physiology; D004622:Embryo, Mammalian; D006624:Hippocampus / Q000166:cytology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology*; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection / Q000379:methods; D020798:Two-Hybrid System Techniques
chemical_list: D000089982:ADP-Ribosylation Factor 6; D002352:Carrier Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C506603:vezatin protein, mouse; D020727:ADP-Ribosylation Factors; C000715727:Arf6 protein, mouse