Highly sensitive non-stripping gold nanoparticles-based chemiluminescent detection of DNA hybridization coupled to magnetic beads.
We have developed a new nanoparticle (NP)-based chemiluminescent (CL) method for the sensitive detection of DNA hybridization. The "sandwich-type" DNA technique comprises NH(2)-functionalized capture DNA immobilized on magnetic beads that can hybridize with one end of the target DNA. The other end of the target is recognized by a reporter DNA probe labeled with Au NPs on its 3-terminus. The hybridization events were monitored by a simple and direct luminol-AgNO(3) CL reaction with a detection limit of 0.5 pM through a non-stripping Au NPs catalysis. Moreover, the CL reaction of non-stripping Au NPs-luminol-AgNO(3) offers the advantages of being simpler and faster as compared to previous stripping Ag NPs and CuS methods. This simple and easy-to-use technique holds great promise for clinical applications of DNA hybridization.
Cai
Sheng
S
School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai, 201203, China.
Xin
Liang
L
Lau
Choiwan
C
Lu
Jianzhong
J
D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012835:Silver Nitrate / Q000737:chemistry
D015342:DNA Probes; D008165:Luminol; D006046:Gold; D004247:DNA; D012835:Silver Nitrate