Effects of osthole on apoptosis and TGF-beta1 of hypertrophic scar fibroblasts.
Osthole, 7-methoxy-8-[3-methylpent-2-enyl]coumarin (1), was extracted from a Chinese herb Cnidium monnieri (L.) Cuss. It showed immunity strengthening, anti-tumor, anti-hepatitis, and anti-osteoporosis activities in previous studies. Our goals are to study the effects of 1 on cell proliferation and TGF-beta of hypertrophic scar fibroblasts. Our results showed that 1 induced apoptosis and inhibited cell proliferation in hypertrophic scar fibroblasts. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that its IC(50) value toward hypertrophic scar fibroblasts was 15.5 +/- 2.2 micromol/l. Furthermore, the results of cell growth curve matched with the above results. Inducing apoptosis by 1 in hypertrophic scar fibroblasts was assessed by various morphological and biochemical characteristics, including cell shrinkage, chromatin condensation, membrane blebbing, formation of apoptotic bodies, and DNA ladder formation. A typical 'Sub-G(1) peak' was also checked through flow cytometry. We used immunohistochemistry to observe the expression of TGF-beta(1). Also, we found that 1 could obviously inhibit the expression of TGF-beta(1) of fibroblasts derived from hypertrophic scar compared with the control group (P < 0.05). These results suggest that 1 inhibits the growth of hypertrophic scar fibroblasts through apoptosis and decreases the expression of TGF-beta(1).
Hou
Xiao-Hua
XH
Affiliated Hospital of Medical College of Chinese People's Armed Police Forces, Tianjin, China.
Cao
Bo
B
Liu
Hong-Qi
HQ
Wang
Yi-Zheng
YZ
Bai
Shu-Fang
SF
Chen
Hong
H
D017209:Apoptosis / Q000187:drug effects; D017439:Cicatrix, Hypertrophic / Q000473:pathology; D003374:Coumarins / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D003374:Coumarins; D004365:Drugs, Chinese Herbal; D053773:Transforming Growth Factor beta1; C046627:osthol