title: Quantitative, reagentless, single-step electrochemical detection of anti-DNA antibodies directly in blood serum.
abstract: Here we demonstrate the use of redox labeled double- and single-stranded oligonucleotides as recognition probes for the reagentless, single-step, electrochemical detection of anti-DNA antibodies directly in blood serum.
authors.affiliation: University of Rome Tor Vergata, Dipartimento di Scienze e Tecnologie Chimiche, Via della Ricerca Scientifica, 00133, Rome, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Ricci
authors.forename: Gianluca
authors.initials: G
authors.lastname: Adornetto
authors.forename: Danila
authors.initials: D
authors.lastname: Moscone
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Plaxco
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Palleschi
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000737:chemistry / Q000276:immunology*; D001774:Blood Chemical Analysis / Q000379:methods*; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000276:immunology*; D015342:DNA Probes / Q000737:chemistry / Q000276:immunology; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D007118:Immunoassay; D057230:Limit of Detection; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D019095:Point-of-Care Systems; D011487:Protein Conformation; D013997:Time Factors
chemical_list: D000906:Antibodies; D015342:DNA Probes; D006046:Gold; D004247:DNA