Generation of E3-deleted canine adenovirus type 2 expressing the Gc glycoprotein of Seoul virus by gene insertion or deletion of related terminal region sequences.
Seoul virus (SEOV) is one of the four hantaviruses known to cause haemorrhagic fever with renal syndrome. The medium genome segment encodes the Gn/Gc glycoproteins of SEOV, which form the major structural part of the virus envelope. Gc and/or Gn are the candidate antigens of hantavirus for induction of a highly immunogenic response for hantavirus vaccine. In this study, the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the Gc protein of SEOV was evaluated in BALB/c mice. Sera from immunized mice contained neutralizing antibodies that could specifically recognize SEOV and neutralize its infectivity in vitro. Moreover, the recombinant virus induced complete protection against an intensive infectious challenge with approximately 1000 50 % infective doses for SEOV strain CC-2. Protective-level neutralizing antibodies were maintained for at least 20 weeks. This recombinant virus is therefore a potential alternative to the inactivated vaccine.
Yuan
Zi-Guo
ZG
College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou 510642, Guangdong Province, PR China. ziguoyuan@scau.edu.cn
Luo
Sheng-Jun
SJ
Xu
Hui-Juan
HJ
Wang
Xiao-Hu
XH
Li
Juan
J
Yuan
Li-Guo
LG
He
Le-Tian
LT
Zhang
Xiu-Xiang
XX
D017503:Adenovirus E3 Proteins / Q000235:genetics*; D006516:Adenoviruses, Canine / Q000235:genetics*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D004285:Dogs; D005260:Female; D017353:Gene Deletion; D015967:Gene Expression Regulation, Viral; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D029263:Seoul virus / Q000235:genetics*; D013047:Specific Pathogen-Free Organisms; D014614:Vaccines, Synthetic / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D017503:Adenovirus E3 Proteins; D000914:Antibodies, Viral; D006023:Glycoproteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines