title: Viral shedding after p53 adenoviral gene therapy in 10 cases of esophageal cancer.
abstract: We detected adenoviral DNA fragments in excretions of 10 esophageal cancer patients by DNA-PCR after tumor injection of Ad-CMV-vector. A total of 220 samples consisting of feces, gargling saliva, urine, and blood plasma were assessed. A total of 29.7% of feces samples and 13.2% of gargling saliva samples were positive for adenoviral DNA fragments, but 89.7% of the positive feces samples and all of the positive gargling saliva samples turned negative on day 12 after tumor injection. Although adenoviral DNA fragments may be pathogen-free, patients' feces and gargling saliva contain adenoviral DNA fragments for 12 days after injection.
authors.affiliation: Department of Frontier Surgery, Chiba University Graduate School of Medicine, Chuoh-ku, Chiba, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawahira
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Matsushita
authors.forename: Tooru
authors.initials: T
authors.lastname: Shiratori
authors.forename: Takanori
authors.initials: T
authors.lastname: Shimizu
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nabeya
authors.forename: Hideki
authors.initials: H
authors.lastname: Hayashi
authors.forename: Takenori
authors.initials: T
authors.lastname: Ochiai
authors.forename: Hisahiro
authors.initials: H
authors.lastname: Matsubara
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shimada
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000628:therapy* / Q000821:virology*; D016158:Genes, p53; D015316:Genetic Therapy; D006801:Humans; D016133:Polymerase Chain Reaction; D017201:Virus Shedding