title: Novel duplex real-time PCR assay detects Bordetella holmesii in specimens from patients with Pertussis-like symptoms in Ontario, Canada.
abstract: Bordetella holmesii is a human pathogen found mainly in immunocompromised patients. A specific real-time PCR assay was developed and successfully used to identify specimens from which B. holmesii was misidentified as Bordetella pertussis and to establish the prevalence of B. holmesii in Ontario patients with pertussis-like symptoms.
authors.affiliation: Ontario Agency for Health Protection and Promotion, Toronto, Ontario M9P 3T1, Canada. jennifer.guthrie@oahpp.ca
authors.forename: J L
authors.initials: JL
authors.lastname: Guthrie
authors.forename: A V
authors.initials: AV
authors.lastname: Robertson
authors.forename: P
authors.initials: P
authors.lastname: Tang
authors.forename: F
authors.initials: F
authors.lastname: Jamieson
authors.forename: S J
authors.initials: SJ
authors.lastname: Drews
mesh_terms: D001884:Bordetella / Q000145:classification / Q000302:isolation & purification*; D001885:Bordetella Infections / Q000453:epidemiology* / Q000382:microbiology* / Q000473:pathology; D006801:Humans; D009864:Ontario / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D012680:Sensitivity and Specificity