title: Protection of nonhuman primates against two species of Ebola virus infection with a single complex adenovirus vector.
abstract: Ebola viruses are highly pathogenic viruses that cause outbreaks of hemorrhagic fever in humans and other primates. To meet the need for a vaccine against the several types of Ebola viruses that cause human diseases, we developed a multivalent vaccine candidate (EBO7) that expresses the glycoproteins of Zaire ebolavirus (ZEBOV) and Sudan ebolavirus (SEBOV) in a single complex adenovirus-based vector (CAdVax). We evaluated our vaccine in nonhuman primates against the parenteral and aerosol routes of lethal challenge. EBO7 vaccine provided protection against both Ebola viruses by either route of infection. Significantly, protection against SEBOV given as an aerosol challenge, which has not previously been shown, could be achieved with a boosting vaccination. These results demonstrate the feasibility of creating a robust, multivalent Ebola virus vaccine that would be effective in the event of a natural virus outbreak or biological threat.
authors.affiliation: U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter St., Fort Detrick, MD 21702-5011, USA. williamd.pratt@us.army.mil
authors.forename: William D
authors.initials: WD
authors.lastname: Pratt
authors.forename: Danher
authors.initials: D
authors.lastname: Wang
authors.forename: Donald K
authors.initials: DK
authors.lastname: Nichols
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.forename: Jan
authors.initials: J
authors.lastname: Woraratanadharm
authors.forename: John M
authors.initials: JM
authors.lastname: Dye
authors.forename: David H
authors.initials: DH
authors.lastname: Holman
authors.forename: John Y
authors.initials: JY
authors.lastname: Dong
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D004195:Disease Models, Animal; D046129:Ebola Vaccines / Q000235:genetics / Q000276:immunology*; D029043:Ebolavirus / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology / Q000517:prevention & control*; D006801:Humans; D007117:Immunization, Secondary / Q000379:methods; D008252:Macaca fascicularis; D008253:Macaca mulatta; D016019:Survival Analysis
chemical_list: D046129:Ebola Vaccines