title: Therapeutic angiogenesis in patients with systemic sclerosis by autologous transplantation of bone-marrow-derived cells.
abstract: We examined the efficacy and safety of autologous transplantation of bone-marrow-derived cells in patients with intractable ulcers caused by systemic sclerosis. Eight patients with ulcers resistant to treatment were enrolled. Bone marrow cells were gathered from the bilateral iliac crests with multiple repositioning bone marrow needles, and bone-marrow-derived mononuclear cells were isolated and injected into skeletal muscles of the ischemic limb. Visual analog scale (VAS), Sclerosis Health Assessment Questionnaire (SHAQ), modified Rodnan total skin score (mTSS), and the size and depth of the ulcer were examined. Thermography, capillaroscopy, intra-arterial digital subtraction angiography (IA-DSA), and laser Doppler flowmetry were also examined before and after transplantation. In all patients, reduction of ulcer size and improvement of VAS were observed after treatment. Elevation of surface temperature, increase of blood flow volume, and new capillaries of the nail bed were also found after our treatment. There were no major adverse effects of this treatment. Autologous transplantation of bone-marrow-derived cells was shown to be a novel and useful approach to intractable ulcers in systemic sclerosis.
authors.affiliation: Department of Internal Medicine and Clinical Immunology, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan. ishigats@med.yokohama-cu.ac.jp
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Ishigatsubo
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ihata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Maasa
authors.initials: M
authors.lastname: Hama
authors.forename: Yohei
authors.initials: Y
authors.lastname: Kirino
authors.forename: Atsuhisa
authors.initials: A
authors.lastname: Ueda
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Takeno
authors.forename: Akira
authors.initials: A
authors.lastname: Shirai
authors.forename: Shigeru
authors.initials: S
authors.lastname: Ohno
mesh_terms: D000328:Adult; D000368:Aged; D016026:Bone Marrow Transplantation; D005260:Female; D006801:Humans; D017078:Laser-Doppler Flowmetry; D020678:Microscopic Angioscopy; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D010147:Pain Measurement; D018579:Patient Selection; D012595:Scleroderma, Systemic / Q000150:complications / Q000601:surgery*; D012883:Skin Ulcer / Q000209:etiology / Q000601:surgery*; D011795:Surveys and Questionnaires; D014182:Transplantation, Autologous; D016896:Treatment Outcome