title: Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid.
abstract: We demonstrate, using transmission electron microscopy and immunopurification with an antibody specific for RNA/DNA hybrid, that intact mitochondrial DNA replication intermediates are essentially duplex throughout their length but contain extensive RNA tracts on one strand. However, the extent of preservation of RNA in such molecules is highly dependent on the preparative method used. These findings strongly support the strand-coupled model of mitochondrial DNA replication involving RNA incorporation throughout the lagging strand.
authors.affiliation: Department of Forensic Medicine and Tampere University Hospital, FI-33014 University of Tampere, Finland.
authors.forename: Jaakko L O
authors.initials: JL
authors.lastname: Pohjoismäki
authors.forename: J Bradley
authors.initials: JB
authors.lastname: Holmes
authors.forename: Stuart R
authors.initials: SR
authors.lastname: Wood
authors.forename: Ming-Yao
authors.initials: MY
authors.lastname: Yang
authors.forename: Takehiro
authors.initials: T
authors.lastname: Yasukawa
authors.forename: Aurelio
authors.initials: A
authors.lastname: Reyes
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Bailey
authors.forename: Tricia J
authors.initials: TJ
authors.lastname: Cluett
authors.forename: Steffi
authors.initials: S
authors.lastname: Goffart
authors.forename: Smaranda
authors.initials: S
authors.lastname: Willcox
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Rigby
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Jackson
authors.forename: Johannes N
authors.initials: JN
authors.lastname: Spelbrink
authors.forename: Jack D
authors.initials: JD
authors.lastname: Griffith
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Crouch
authors.forename: Howard T
authors.initials: HT
authors.lastname: Jacobs
authors.forename: Ian J
authors.initials: IJ
authors.lastname: Holt
mesh_terms: D000818:Animals; D004247:DNA; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000737:chemistry*; D006801:Humans; D008322:Mammals; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012313:RNA
chemical_list: D004272:DNA, Mitochondrial; D012313:RNA; D004247:DNA