title: Embryo development of porcine oocytes after injection with miniature pig sperm and their extracts.
abstract: This study examined embryo development of porcine oocytes after microinjection of sperm extracts (SE) in porcine intracytoplasmic sperm injection (ICSI). SE was prepared from miniature pig sperm by a nonionic surfactant, and various concentrations (0.02, 0.04 and 0.08 mg/mL) of SE were injected into the matured oocytes with a first polar body. In the pronuclear stage, the rate of oocytes with two pronuclei and a second polar body (21.4%) in the sperm and SE (0.04 mg/mL) injection group was significantly higher (P < 0.05) compared to other groups. The rate of 2-4-cell stage in sperm and SE (0.04 mg/mL) injection group was 38.1%, and it was significantly higher than that in the sperm injection group (22.9%). The rate of blastocyst stage in sperm and SE (0.04 mg/mL) injection group was 21.4%, the value was significantly higher than those in SE (0.08 mg/mL) injection group (0%), sperm injection group (5.7%), and sperm and SE (0.08 mg/mL) injection group (2.6%). These results suggest that SE induces activation of porcine oocytes and their further embryonic development, and that SE is effective for porcine ICSI.
authors.affiliation: Suzuka Kaisei General Hospital, Suzuka, Japan.
authors.forename: Daizou
authors.initials: D
authors.lastname: Matsuura
authors.forename: Teruo
authors.initials: T
authors.lastname: Maeda
mesh_terms: D000818:Animals; D047108:Embryonic Development; D005260:Female; D008297:Male; D008845:Microinjections; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods; D013094:Spermatozoa / Q000502:physiology*; D013552:Swine / Q000196:embryology*; D013556:Swine, Miniature