Improvement of the extraction efficiency of D-amygdalin from Armeniacae Semen powder through inactivating emulsin and suppressing the epimerization of D-amygdalin.
Armeniacae Semen contains not only amygdalin but also emulsin, which is an enzyme that hydrolyzes amygdalin. This hydrolysis reaction has been a major problem associated with the water extraction of Armeniacae Semen powder. In this study, the emulsin was inactivated by extracting Armeniacae Semen powder at a constant temperature of 90 degrees C. In addition, in order to suppress the epimerization of D-amygdalin, the extraction time was kept to less than 8 min. The use of a 10 mM sodium phosphate buffer (pH 2.3) containing 13.5% acetonitrile as a mobile phase in reversed-phase HPLC was effective in separating and analyzing the D-amygdalin and neoamygdalin. The linearity between the concentrations and detector responses was obtained in the range of 0.05 to 0.5 mM. The detection limits for D-amygdalin and neoamygdalin were approximately 5 microM per amount injected.
Kwon
Ha-Jeong
HJ
Department of Preventive and Social Dentistry & Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Korea.
Lee
Je-Hyun
JH
Hong
Seon-Pyo
SP
D000678:Amygdalin / Q000737:chemistry / Q000302:isolation & purification*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D007202:Indicators and Reagents; D007536:Isomerism; D010936:Plant Extracts / Q000737:chemistry; D027861:Prunus / Q000737:chemistry*; D012015:Reference Standards; D012639:Seeds / Q000737:chemistry; D013696:Temperature
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D007202:Indicators and Reagents; D010936:Plant Extracts; D000678:Amygdalin