Use of the kinase inhibitor analog 1NM-PP1 reveals a role for Toxoplasma gondii CDPK1 in the invasion step.
Toxoplasma gondii CDPK1 (TgCDPK1) was found to be the target of the toxoplasmocidal compound 1NM-PP1. When TgCDPK1 was mutated at position 128 from glycine to methionine, resistance was gained. Inhibition of gliding motility without inhibition of micronemal secretion by 1NM-PP1 suggests a function for TgCDPK1 in gliding motility.
Sugi
Tatsuki
T
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Kato
Kentaro
K
Kobayashi
Kyosuke
K
Watanabe
Shumpei
S
Kurokawa
Hitomi
H
Gong
Haiyan
H
Pandey
Kishor
K
Takemae
Hitoshi
H
Akashi
Hiroomi
H
D000595:Amino Acid Sequence; D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D011247:Pregnancy; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D011720:Pyrazoles / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D016415:Sequence Alignment; D014122:Toxoplasma / Q000187:drug effects* / Q000502:physiology*
C479693:1-tert-butyl-3-naphthalen-1-ylmethyl-1H-pyrazolo(3,4-d)pyrimidin-4-ylemine; D047428:Protein Kinase Inhibitors; D015800:Protozoan Proteins; D011720:Pyrazoles; D011743:Pyrimidines; D011494:Protein Kinases