title: Donor-derived DNA in hair follicles of recipients after allogeneic hematopoietic stem cell transplantation.
abstract: The hair follicles of recipients of allogeneic hematopoietic SCT (HSCT) constitute the tissue with the greatest need for regeneration after high-dose chemotherapy. Previous studies have shown a lack of donor-derived DNA in the hair follicles of recipients. Therefore, we carried out a study to determine whether male donor-derived genetic material can be found in female recipients' hair follicles after HSCT. Fluorescent-based PCR with analyses of Y-chromosome STR (Y-STR) and RQ-PCR with the sex-determining region Y (SRY) were used independently to evaluate chimerism status. Our results proved the existence of donor-derived stem DNA in the recipients' hair follicle cells. This report undermines the validity of data indicating that hair follicle cells maintain 100% of recipient origin.
authors.affiliation: Forensic Genetics Laboratory, Department of Forensic Medicine, Medical University of Lodz, Sedziowska 18a, Lodz, Poland. r.jacewicz@post.pl
authors.forename: R
authors.initials: R
authors.lastname: Jacewicz
authors.forename: K
authors.initials: K
authors.lastname: Lewandowski
authors.forename: J
authors.initials: J
authors.lastname: Rupa-Matysek
authors.forename: M
authors.initials: M
authors.lastname: Jedrzejczyk
authors.forename: P M
authors.initials: PM
authors.lastname: Brzezinski
authors.forename: T
authors.initials: T
authors.lastname: Dobosz
authors.forename: A
authors.initials: A
authors.lastname: Jonkisz
authors.forename: S
authors.initials: S
authors.lastname: Szram
authors.forename: M
authors.initials: M
authors.lastname: Komarnicki
authors.forename: J
authors.initials: J
authors.lastname: Berent
mesh_terms: D000328:Adult; D041322:Chromosomes, Human, Y; D004247:DNA / Q000032:analysis / Q000235:genetics*; D005260:Female; D006085:Graft Survival; D018859:Hair Follicle / Q000737:chemistry / Q000502:physiology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D014019:Tissue Donors; D018183:Transplantation Chimera; D055815:Young Adult
chemical_list: D004247:DNA