Fibroblasts express immune relevant genes and are important sentinel cells during tissue damage in rainbow trout (Oncorhynchus mykiss).
Fibroblasts have shown to be an immune competent cell type in mammals. However, little is known about the immunological functions of this cell-type in lower vertebrates. A rainbow trout hypodermal fibroblast cell-line (RTHDF) was shown to be responsive to PAMPs and DAMPs after stimulation with LPS from E. coli, supernatant and debris from sonicated RTHDF cells. LPS was overall the strongest inducer of IL-1beta, IL-8, IL-10, TLR-3 and TLR-9. IL-1beta and IL-8 were already highly up regulated after 1 hour of LPS stimulation. Supernatant stimuli significantly increased the expression of IL-1beta, TLR-3 and TLR-9, whereas the debris stimuli only increased expression of IL-1beta. Consequently, an in vivo experiment was further set up. By mechanically damaging the muscle tissue of rainbow trout, it was shown that fibroblasts in the muscle tissue of rainbow trout contribute to electing a highly local inflammatory response following tissue injury. The damaged muscle tissue showed a strong increase in the expression of the immune genes IL-1beta, IL-8 and TGF-beta already 4 hours post injury at the site of injury while the expression in non-damaged muscle tissue was not influenced. A weaker, but significant response was also seen for TLR-9 and TLR-22. Rainbow trout fibroblasts were found to be highly immune competent with a significant ability to express cytokines and immune receptors. Thus fish fibroblasts are believed to contribute significantly to local inflammatory reactions in concert with the traditional immune cells.
Ingerslev
Hans-Christian
HC
Section for Aquatic Protein Biochemistry, Division for Seafood Research, DTU Food, National Food Institute, Lyngby, Denmark. hci@aqua.dtu.dk
Ossum
Carlo Gunnar
CG
Lindenstrøm
Thomas
T
Nielsen
Michael Engelbrecht
ME
D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D029941:Fish Proteins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D053583:Interleukin-1beta / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D009132:Muscles / Q000276:immunology / Q000293:injuries / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013314:Stress, Mechanical; D013997:Time Factors; D051196:Toll-Like Receptor 3 / Q000235:genetics; D051217:Toll-Like Receptor 9 / Q000235:genetics
D029941:Fish Proteins; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D051196:Toll-Like Receptor 3; D051217:Toll-Like Receptor 9