title: Cyclooxygenase-2 in testes of infertile men: evidence for the induction of prostaglandin synthesis by interleukin-1β.
abstract: As we previously reported, testes of men suffering from hypospermatogenesis and germ cell arrest or Sertoli cell-only syndrome show a major increase in the number of macrophages expressing interleukin-1β (IL-1β) and abundant expression of cyclooxygenase-2 (COX-2), the inducible isoform of the key enzyme in the biosynthesis of prostaglandins (PGs), in Leydig cells. In the present study we report [1] a positive correlation between IL-1β levels and COX-2 expression in testes of infertile patients, [2] the induction of COX-2 by IL-1β in mouse Leydig cells (TM3) and human macrophages (THP-1), and therefore [3] evidence for an IL-1β-dependent induction of testicular inflammatory states.
authors.affiliation: Laboratorio de Esteroides, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Matzkin
authors.forename: Artur
authors.initials: A
authors.lastname: Mayerhofer
authors.forename: Soledad Paola
authors.initials: SP
authors.lastname: Rossi
authors.forename: Betina
authors.initials: B
authors.lastname: Gonzalez
authors.forename: Candela Rocío
authors.initials: CR
authors.lastname: Gonzalez
authors.forename: Silvia Inés
authors.initials: SI
authors.lastname: Gonzalez-Calvar
authors.forename: Claudio
authors.initials: C
authors.lastname: Terradas
authors.forename: Roberto
authors.initials: R
authors.lastname: Ponzio
authors.forename: Elisa
authors.initials: E
authors.lastname: Puigdomenech
authors.forename: Oscar
authors.initials: O
authors.lastname: Levalle
authors.forename: Ricardo Saúl
authors.initials: RS
authors.lastname: Calandra
authors.forename: Mónica Beatriz
authors.initials: MB
authors.lastname: Frungieri
mesh_terms: D000328:Adult; D000818:Animals; D001706:Biopsy; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism* / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism* / Q000494:pharmacology; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D011453:Prostaglandins / Q000378:metabolism*; D054331:Sertoli Cell-Only Syndrome / Q000378:metabolism / Q000473:pathology; D013737:Testis / Q000378:metabolism* / Q000473:pathology
chemical_list: D053583:Interleukin-1beta; D011453:Prostaglandins; D051546:Cyclooxygenase 2