[Antioxidative response of Phytolacca americana and Nicotiana tabacum to manganese stresses].
Plant species capable of accumulating heavy metals are of considerable interest for phytoremediation and phytomining. The mechanism of Mn tolerance/hyperaccumulate in Phytolacca americana L. is less known. To elucidate the role of antioxidative enzyme in response to Mn, the 6-week-old seedling of Mn hyperaccumulator P. americana and non-accumulator-tobacco (Nicotiana tabacum) were exposed to half strength Hoagland solution with 1 mmol x L(-1) or 3 mmol x L(-1) MnCl2 for 4 days. The photosynthetic rate in P. americana decreased more slowly than that in tobacco, while the MDA content and electrolyte leakage in tobacco increased more rapidly than that in P. americana. For example, after exposure to 1 mmol x L(-1) Mn for 4 days, the photosynthetic rates of P. americana and tobacco in comparison to the control reduced by 13.3% and 75.5%, respectively. The MDA content and electrolyte leakage in tobacco increased by 347.3% and 120.1%, respectively, whereas Mn had no marked effect on both of it in P. americana, indicated that the oxidative damage in tobacco was more serious than that in P. americana. The activities of SOD and POD of both species increased rapidly with elevated Mn concentration and exposure time in both species, the increase of SOD activity in P. americana was higher than that in tobacco. CAT activity in tobacco declined rapidly, while the activity of CAT in P. americana was increased. The activities of SOD, POD and CAT in P. americana upon 1 mmol x L(-1) Mn exposure increased by 161.1%, 111.3% and 17.5%, respectively. The activities of SOD and POD in tobacco increased by 55.5% and 206.0%, respectively, while CAT activity decreased by 15.6%, indicating that the antioxidative enzymes in P. americana, particularly in CAT,could fully scavenge the reactive oxygen species generated by Mn toxicity. These results collectively indicate that the enzymatic antioxidation capacity is one of the important mechanisms responsible for Mn tolerance in hyperaccumulator plant species.
Zhang
Yu-xiu
YX
Department of Bioengineering, School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijing 100083, China. zhangyuxiu@cumtb.edu.cn
Huang
Zhi-bo
ZB
Zhang
Hong-mei
HM
Li
Lin-feng
LF
Chai
Tuan-yao
TY
D000975:Antioxidants / Q000378:metabolism*; D001673:Biodegradation, Environmental; D008345:Manganese / Q000633:toxicity*; D010544:Peroxidases / Q000378:metabolism; D029604:Phytolacca americana / Q000378:metabolism / Q000502:physiology*; D036226:Seedlings / Q000502:physiology; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism / Q000502:physiology*
D000975:Antioxidants; D008345:Manganese; D010544:Peroxidases; D013482:Superoxide Dismutase