Comparison of signal-to-noise, blank determination, and linear regression methods for the estimation of detection and quantification limits for volatile organic compounds by gas chromatography.
LOD and LOQ are two important performance characteristics in method validation. This work compares three methods based on the International Conference on Harmonization and EURACHEM guidelines, namely, signal-to-noise, blank determination, and linear regression, to estimate the LOD and LOQ for volatile organic compounds (VOCs) by experimental methodology using GC. Five VOCs, toluene, ethylbenzene, isopropylbenzene, n-propylbenzene, and styrene, were chosen for the experimental study. The results indicated that the estimated LODs and LOQs were not equivalent and could vary by a factor of 5 to 6 for the different methods. It is, therefore, essential to have a clearly described procedure for estimating the LOD and LOQ during method validation to allow interlaboratory comparisons.
Sanagi
M Marsin
MM
Universiti Teknologi Malaysia, Department of Chemistry, Faculty of Science, 81310 UTM Skudai, Johor, Malaysia. marsin@kimia.fs.utm.my
Ling
Susie L
SL
Nasir
Zalilah
Z
Hermawan
Dadan
D
Ibrahim
Wan Aini Wan
WA
Abu Naim
Ahmedy
A
D000465:Algorithms; D002849:Chromatography, Gas; D003627:Data Interpretation, Statistical; D007202:Indicators and Reagents; D016018:Least-Squares Analysis; D016014:Linear Models; D009930:Organic Chemicals / Q000032:analysis*; D012015:Reference Standards
D007202:Indicators and Reagents; D009930:Organic Chemicals