Cytotoxic phenolic compounds from Chionanthus retusus.
Cytotoxicity-guided fractionation and purification of MeOH extract from Chionanthus retusus Lindl. et Paxton resulted in the isolation of compounds. Fourteen phenolic compounds were isolated from the EtOAc soluble fraction, and their structures were determined by spectroscopic analysis. Isolated compounds were identified as phillygenin (1), scopoletin (2), pinoresinol (3), kaempferol (4), aromadendrin (5), 2-(4-hydroxyphenyl)ethanol (6), 3,3',5,5',7-pentahydroxyflavanone (7), luteolin (8), quercetin (9), apigenin (10), chrysoeriol (11), phillyrin (12), oleuropein (13), (7R,8R)-guaiacylglycerol (14). All compounds except for 12 and 13 were isolated for the first time from this plant and genus Chionanthus. Anti-proliferative effect of isolated compounds were evaluated by the sulforhodamin B assay against four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and HCT15). Compounds 4 and 8-10 were significantly active with ED50 values of 1.84-6.35 microg/mL. Also, compounds 1, 3, 5 and 7 revealed cytotoxic effects at concentrations below 30 microg/mL.
Kwak
Jong Hwan
JH
College of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Korea. jhkwak@skku.edu
Kang
Min Woo
MW
Roh
Jong Hwa
JH
Choi
Sang Un
SU
Zee
Ok Pyo
OP
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol; D029592:Oleaceae / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000903:Antibiotics, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents; D004317:Doxorubicin; D000432:Methanol