title: The generation of GLP-grade human embryonic stem cell banks from four clinical-grade cell lines for preclinical research.
abstract: The Singapore Stem Cell Bank has generated human embryonic stem cell banks from clinical-grade cell lines ESI-017, ESI-035, ESI-049, and ESI-053. All banks were prepared and characterized according to principles of Good Laboratory Practice for quality assurance. Importantly, each cell line has clearly documented and approved ethical provenance and meets recognized standards for performance and safety. The banks are intended to facilitate the translation of stem cell research to clinical medicine by enabling early phase research and development with high-quality, low-cost cells that are also available as clinical-grade stocks.
authors.affiliation: Singapore Stem Cell Bank, Singapore Stem Cell Consortium, A*STAR, Singapore, Singapore.
authors.forename: Shirani
authors.initials: S
authors.lastname: Sivarajah
authors.forename: Grace Selva
authors.initials: GS
authors.lastname: Raj
authors.forename: Adam Joshua Varughese
authors.initials: AJ
authors.lastname: Mathews
authors.forename: Norliza Binte Esmail
authors.initials: NB
authors.lastname: Sahib
authors.forename: Wei Sek
authors.initials: WS
authors.lastname: Hwang
authors.forename: Jeremy Micah
authors.initials: JM
authors.lastname: Crook
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D033041:Embryo Research; D053595:Embryonic Stem Cells / Q000166:cytology*; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014015:Tissue Banks / Q000592:standards*
chemical_list: D015415:Biomarkers