title: Test compounds for detecting the silanol effect on the elution of ionized amines in reversed-phase LC.
abstract: The effectiveness of several basic compounds for testing silica-based stationary phases was reviewed by applying them to recent columns for reversed-phase HPLC. Most octadecylsilylated (C18) stationary phases, prepared as a base-deactivated material from high-purity silica gel with endcapping, provided excellent peak shape and column efficiency for the bases including benzylamine and amitriptyline that once caused problems and were subsequently employed for testing silanol activities. However, a cyclic tertiary amine, dextrometorphan, was eluted as an acceptable peak from only a few columns at neutral pH. Such a more sensitive probe is expected to contribute to further improvement of the stationary phase for reversed-phase HPLC.
authors.affiliation: GL Sciences Inc., Iruma, Saitama, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Okusa
authors.forename: Yuki
authors.initials: Y
authors.lastname: Suita
authors.forename: Yukio
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Mineo
authors.initials: M
authors.lastname: Tahara
authors.forename: Tohru
authors.initials: T
authors.lastname: Ikegami
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tanaka
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Ohira
authors.forename: Masakazu
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000588:Amines / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D007477:Ions / Q000737:chemistry / Q000302:isolation & purification; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000588:Amines; D007477:Ions; D012821:Silanes; C082343:silanol; D012822:Silicon Dioxide