Novel method to detect a construct-specific sequence of the acetolactate synthase gene in genetically-modified flax CDC Triffid (FP967).
During the fall of 2009, a trace of unauthorized genetically modified (GM) flax (Linum usitatissimum L.) line, CDC Triffid, which is resistant to sulfonylurea herbicides, was detected in many countries including Japan. A method to reliably identify the CDC Triffid line was urgently required. We developed a novel construct-specific real-time polymerase chain reaction (PCR) method to identify the mutant acetolactate synthase gene in the CDC Triffid line. We confirmed that the method can detect 0.001% GM flax in DNA mixing solution. The study shows that the developed method is specific, sensitive and reliable way to monitor a trace of CDC Triffid.
Nakamura
Kosuke
K
National Institute of Health Sciences, Tokyo 158-8501, Japan.
Akiyama
Hiroshi
H
Yamada
Chihiro
C
Satoh
Rie
R
Makiyama
Daiki
D
Sakata
Kozue
K
Kawakami
Hiroshi
H
Mano
Junichi
J
Kitta
Kazumi
K
Teshima
Reiko
R
D000095:Acetolactate Synthase / Q000235:genetics*; D001483:Base Sequence; D018744:DNA, Plant; D019597:Flax / Q000235:genetics*; D017343:Genes, Plant; D009154:Mutation; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*
D018744:DNA, Plant; D000095:Acetolactate Synthase