Improvement of malignant serous effusions diagnosis by quantitative analysis of molecular claudin 4 expression.
Claudin gene expression is frequently altered in human cancers. Our aim was to improve the cytology diagnosis of malignancy in serous fluids with the quantification of claudins compared with various classic molecular markers using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) method. Peritoneal or pleural effusions of 56 patients were assessed as malignant from histological analysis and 19 as benign. Claudin 4 was the most significantly upregulated (68%) marker in patients with malignant effusions. In cytologically negative malignant effusions, claudin 4 was found increased in 8/18 fluids. Quantitative RT-PCR is a sensitive method for the detection of free cancer cells in serous effusions.
Mohamed
Fauzia
F
Department of Immunology, University Hospital Center, Saint-Etienne, France.
Vincent
Nadine
N
Cottier
Michèle
M
Peoc'h
Michel
M
Merrouche
Yacine
Y
Patouillard
Brigitte
B
Paul
Stéphane
S
Genin
Christian
C
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism; D001202:Ascitic Fluid / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D062445:Claudin-1; D062506:Claudin-4; D057167:Claudins; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D006801:Humans; D053539:Keratin-19 / Q000235:genetics / Q000378:metabolism; D053554:Keratin-20 / Q000235:genetics / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D018396:Mucin-1 / Q000235:genetics / Q000378:metabolism; D009077:Mucins / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D016066:Pleural Effusion, Malignant / Q000175:diagnosis* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C568108:CLDN1 protein, human; C486683:CLDN18 protein, human; C568157:CLDN4 protein, human; D015815:Cell Adhesion Molecules; D062445:Claudin-1; D062506:Claudin-4; D057167:Claudins; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D053539:Keratin-19; D053554:Keratin-20; C482349:MUC19 protein, human; D008565:Membrane Proteins; D018396:Mucin-1; D009077:Mucins; D011956:Receptors, Cell Surface; C065686:carcinoembryonic antigen binding protein, human