Positron emission tomography imaging of DMBA/TPA mouse skin multi-step tumorigenesis.
Many tumor cells have elevated rates of glucose uptake that can be measured quantitatively, noninvasively and repeatedly by positron emission tomography (PET) with 2-deoxy-2-[(18)F]-fluoro-D-glucose ((18)F-FDG). Clinical imaging with (18)F-FDG PET has been used for detection and staging of primary and metastatic tumors. High-resolution microPET scanning and murine cancer models make it possible to analyze longitudinally glucose metabolism during the appearance, development and progression of individual experimental tumors. In this study, we used (18)F-FDG microPET and micro computerized tomography (microCT) to investigate glucose uptake in the DMBA/TPA chemically-induced multistage mouse skin carcinogenesis model. (18)F-FDG uptake is significantly higher in all papillomas than in surrounding skin. Elevated (18)F-FDG uptake is observed when tumors can be identified morphologically, but not before. Although (18)F-FDG uptake is high in all fully invasive, malignant skin squamous cell carcinomas, uptake in papillomas and microinvasive malignant squamous cell carcinomas is variable and does not exhibit any correlation with tumor stage.
Ishikawa
Tomo-O
TO
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA.
Kumar
Indracanti Prem
IP
Machado
Hidevaldo B
HB
Wong
Koon-Pong
KP
Kusewitt
Donna
D
Huang
Sung-Cheng
SC
Fischer
Susan M
SM
Herschman
Harvey R
HR
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D018450:Disease Progression; D005260:Female; D019788:Fluorodeoxyglucose F18; D005947:Glucose / Q000378:metabolism; D051379:Mice; D010212:Papilloma / Q000139:chemically induced* / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D049268:Positron-Emission Tomography; D011237:Predictive Value of Tests; D019275:Radiopharmaceuticals; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D013755:Tetradecanoylphorbol Acetate / Q000008:administration & dosage / Q000633:toxicity*; D014057:Tomography, X-Ray Computed
D002273:Carcinogens; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D015127:9,10-Dimethyl-1,2-benzanthracene; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate