title: Genome-wide identification of intron fragment insertion mutations and their potential use as SCAR molecular markers in the soybean.
abstract: Introns often have a high probability of mutation as a result of DNA insertions and deletions (indels). In this study, 503 introns with exon-derived insertions were identified using a comprehensive search of the soybean genome. Of the 375 pairs of PCR primer sets designed for the loci in question, 161 primer sets amplified length polymorphism among nine soybean varieties and were identified as soybean gene-intron-driven functional sequence characterized amplified region (SCAR) markers. These SCAR markers are distributed among all 20 of the soybean chromosomes, and they developed from numerous genes involved in various physiological and biochemical processes that influence important agronomic traits of the soybean. The development of these novel gene-driven functional SCAR markers was fast and cost effective, and their use will facilitate molecular-assisted breeding of the soybean.
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, 150030, Heilongjiang, People's Republic of China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Shu
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhu
authors.forename: Zhenlei
authors.initials: Z
authors.lastname: Zhu
authors.forename: Dekang
authors.initials: D
authors.lastname: Lv
authors.forename: Xi
authors.initials: X
authors.lastname: Bai
authors.forename: Hua
authors.initials: H
authors.lastname: Cai
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Dianjing
authors.initials: D
authors.lastname: Guo
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D005819:Genetic Markers; D018745:Genome, Plant; D007438:Introns; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010375:Pedigree; D013025:Glycine max / Q000145:classification / Q000235:genetics*
chemical_list: D005819:Genetic Markers