title: Unprecedented NES non-antagonistic inhibitor for nuclear export of Rev from Sida cordifolia.
abstract: Bioassay-guided separation from the MeOH extract of the South American medicinal plant Sida cordifolia resulted in isolation of (10E,12Z)-9-hydroxyoctadeca-10,12-dienoic acid (1) as an unprecedented NES non-antagonistic inhibitor for nuclear export of Rev. This mechanism of action was established by competitive experiment by the biotinylated probe derived from leptomycin B, the known NES antagonistic inhibitor. Additionally, structure-activity relationship analysis by use of the synthesized analogs clarified cooperation of several functionalities in the Rev-export inhibitory activity of 1.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Tamura
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kaneko
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shiomi
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Yang
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Yamaura
authors.forename: Nobutoshi
authors.initials: N
authors.lastname: Murakami
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015694:Gene Products, rev / Q000378:metabolism*; D019660:Malvaceae / Q000378:metabolism*; D049790:Nuclear Export Signals; D010936:Plant Extracts / Q000494:pharmacology*; D021381:Protein Transport / Q000187:drug effects*
chemical_list: D015694:Gene Products, rev; D049790:Nuclear Export Signals; D010936:Plant Extracts