Maturation of dendritic cell precursors into IL12-producing DCs by J-LEAPS immunogens.
LEAPS (ligand epitope antigen presentation system) vaccines consist of a peptide containing a major histocompatibility antigen binding peptide conjugated to an immune cell binding ligand (ICBL) such as the 'J' peptide from beta-2-microglobulin. Treatment of monocytes, monocytes plus GMCSF, or monocytes plus GMCSF and IL4 with JgD (containing a peptide from gD of herpes simplex virus type 1) or JH (with a peptide from HIV p17 gag protein) was sufficient to promote their maturation into Interleukin 12 producing dendritic cells. JgD-dendritic cells supported allotypic activation of T cells to produce Th1-related cytokines.
Taylor
Patricia R
PR
Northeastern Ohio Universities Colleges of Medicine and Pharmacy, Rootstown, OH 44272, USA.
Paustian
Christopher C
CC
Koski
Gary K
GK
Zimmerman
Daniel H
DH
Rosenthal
Ken S
KS
D017951:Antigen Presentation; D002454:Cell Differentiation; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007144:Immunoglobulin J-Chains / Q000276:immunology*; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000276:immunology
D000939:Epitopes; D007144:Immunoglobulin J-Chains; D018664:Interleukin-12