title: Simultaneous determination of salbutamol sulphate and bromhexine hydrochloride in tablets by reverse phase liquid chromatography.
abstract: A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of salbutamol sulphate and bromhexine hydrochloride. The separation was carried out using a mobile phase consisting of acetonitrile, methanol and phosphate buffer, pH 4 in the ratio 60:20:20 v/v. The column used was SS Wakosil-II C-18 with a flow rate of 1 ml/min and UV detection at 224 nm. The described method was linear over a concentration range of 10-110 mug/ml and 20-140 mug/ml for the assay of salbutamol sulphate and bromhexine hydrochloride, respectively. The mean recovery was found to be 95-105% for salbutamol sulphate and 96.2-102.1% for bromhexine hydrochloride when determined at five different levels.
authors.affiliation: Department of Quality Assurance, Al-Ameen College of Pharmacy, Bangalore-560 027, India.
authors.forename: P N S
authors.initials: PN
authors.lastname: Pai
authors.forename: G K
authors.initials: GK
authors.lastname: Rao
authors.forename: M S
authors.initials: MS
authors.lastname: Murthy
authors.forename: A
authors.initials: A
authors.lastname: Agarwal
authors.forename: S
authors.initials: S
authors.lastname: Puranik