title: Comparison of signal-to-noise, blank determination, and linear regression methods for the estimation of detection and quantification limits for volatile organic compounds by gas chromatography.
abstract: LOD and LOQ are two important performance characteristics in method validation. This work compares three methods based on the International Conference on Harmonization and EURACHEM guidelines, namely, signal-to-noise, blank determination, and linear regression, to estimate the LOD and LOQ for volatile organic compounds (VOCs) by experimental methodology using GC. Five VOCs, toluene, ethylbenzene, isopropylbenzene, n-propylbenzene, and styrene, were chosen for the experimental study. The results indicated that the estimated LODs and LOQs were not equivalent and could vary by a factor of 5 to 6 for the different methods. It is, therefore, essential to have a clearly described procedure for estimating the LOD and LOQ during method validation to allow interlaboratory comparisons.
authors.affiliation: Universiti Teknologi Malaysia, Department of Chemistry, Faculty of Science, 81310 UTM Skudai, Johor, Malaysia. marsin@kimia.fs.utm.my
authors.forename: M Marsin
authors.initials: MM
authors.lastname: Sanagi
authors.forename: Susie L
authors.initials: SL
authors.lastname: Ling
authors.forename: Zalilah
authors.initials: Z
authors.lastname: Nasir
authors.forename: Dadan
authors.initials: D
authors.lastname: Hermawan
authors.forename: Wan Aini Wan
authors.initials: WA
authors.lastname: Ibrahim
authors.forename: Ahmedy
authors.initials: A
authors.lastname: Abu Naim
mesh_terms: D000465:Algorithms; D002849:Chromatography, Gas; D003627:Data Interpretation, Statistical; D007202:Indicators and Reagents; D016018:Least-Squares Analysis; D016014:Linear Models; D009930:Organic Chemicals / Q000032:analysis*; D012015:Reference Standards
chemical_list: D007202:Indicators and Reagents; D009930:Organic Chemicals