Clinical comparison of two Mexican coccidioidins.
Coccidioidin, an extract from the saprophytic mycelial form of Coccidioides spp., has been a very useful antigen preparation both for skin and serological tests for coccidioidomycosis. Unfortunately, coccidioidin is not currently available for skin testing in the United States. Coccidioidin has been produced commercially in Mexico by a vaccine and reagents laboratory of the Mexican Federal Government. It also has been produced at the Microbiology Laboratory of the Faculty of Medicine, Universidad Nacional Autónoma de México exclusively as an antigen for research projects. The objective of the study was to compare both coccidioidins in their reactivity and safety when applied in humans. One hundred and eighty-four volunteers were tested; median age was 33 (range 14-82). When the cutoff point is set in 5 mm, 88 subjects (47.8%) had a positive test for the commercial coccidioidin and 76 (41.3%; CI(95%) 0.50, 1.15; P = 0.20) were positive with the research antigen. Seventy-five subjects were positive for both antigens and 96 were negative for both. Fifty-nine subjects (31.3%) reported an adverse reaction after the application of the antigen; they were mostly very mild local reactions. Mexican research coccidioidin is a safe and reliable antigen that can be used for the detection of coccidioidomycosis infection in mammals.
Castañón-Olivares
Laura Rosío
LR
Micología Médica, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Laniado-Laborín
Rafael
R
Concepcion
Toriello
T
Muñoz-Hernández
Bertha
B
Aroch-Calderón
Arturo
A
Aranda-Uribe
Iván Sammir
IS
Flores-Sánchez
María Antonieta
MA
del Rocío González-Martínez
María
M
Hernández-Navarez
Alfonso
A
Manjarrez-Zavala
María Eugenia
ME
Miranda-Mauricio
Sandra
S
Palma
Gabriel
G
Pérez-Mejía
Amelia
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000946:Antigens, Fungal / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D003045:Coccidioides / Q000276:immunology; D003046:Coccidioidin / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D003047:Coccidioidomycosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D012882:Skin Tests; D014481:United States; D055815:Young Adult
D000946:Antigens, Fungal; D003046:Coccidioidin