Relevance of Bcl-x expression in different types of endometrial tissues.
OBJECTIVE
To explore the roles of Bcl-xl and Bcl-xs in the development and progression of endometrial carcinoma, and to analyze the correlation between Bcl-xl and Bcl-xs.


METHODS
RT-PCR and Western-blot assay were applied to detect the expressions of Bcl-xl and Bcl-xs in endometrial tissues of various histomorphologic types.


RESULTS
The Bcl-xl expression levels of simple and atypical hyperplasia endometrial tissues were not significantly different from that of normal endometrial tissue (both P > 0.05). On contrary, Bcl-xl expression in endometrial carcinoma tissue was significantly higher than the normal endometrial tissue (P = 0.00), which was correlated with the pathological grading of endometrial carcinoma (F = 5.33, P = 0.02). In addition, Bcl-xs mRNA level in simple hyperplasia endometrial tissue had no significant difference compared to that in normal endometrial tissue (P = 0.12), while the levels of atypical hyperplasia and endometrial carcinoma endometrial tissues were significantly different from the normal endometrial tissue (both P = 0.00). Furthermore, level of Bcl-xs mRNA was correlated with the clinical staging and lymph node metastasis of the endometrial carcinoma (P < 0.05). The expressions of Bcl-xl and Bcl-xs were negatively correlated with each other (r = -0.76).


CONCLUSIONS
The abnormal expressions of Bcl-xs and Bcl-xl were one of the molecular mechanisms for the pathogenesis of endometrial carcinoma, and altered ratio between these two might involve in the onset of endometrial carcinoma.
Ma
Xiaoxin
X
Department of Obstetrics & Gynecology, Shengjing Hospital of China Medical University, Shenyang (110004), China. maxiaoxin666@yahoo.com.cn
Zhao
Yanhui
Y
Li
Yanxia
Y
Lu
Hongwei
H
He
Yuanqi
Y
D000328:Adult; D000368:Aged; D004714:Endometrial Hyperplasia / Q000378:metabolism*; D016889:Endometrial Neoplasms / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D051020:bcl-X Protein / Q000378:metabolism*
D012333:RNA, Messenger; D051020:bcl-X Protein