title: 16S-23S rRNA intergenic spacer region variability in the genus Frankia.
abstract: 16S-23S rRNA internally transcribed spacer (ITS) sequences from 53 Frankia strains were sequenced and sized from polymerase chain reaction amplification products and compiled with 14 selected 16S-23S ITS sequences from public database. Frankia genomes included two to three ITS copies lacking length polymorphism except for nine strains. No tRNA gene was encountered in this region. Frankia strains exhibited various lengths (369 to 452 nt) and a wide range of sequence similarity (35-100%) in the ITS region. The average pairwise distance varied from 0.368 (clusters 1 and 2) to 0.964 (clusters 3 and 4) and was 0.397, 0.138, 0.129, and 0.016, respectively, for cluster 4 (saprophytic non-infective/non-effective), clusters 1 and 3 (facultative symbiotic), and cluster 2 (obligate symbiotic). This suggests a gradual erosion of Frankia diversity concomitantly with a shift from saprophytic non-infective/non-effective to facultative and symbiotic lifestyle. Comparative sequence analyses of the 16S-23S rRNA intergenic spacer region of Frankia strains are not useful to assign them to their respective cluster or host infection group. Accurate assignment required the inclusion of the adjacent 16S and 23S rRNA gene fragments.
authors.affiliation: Laboratoire Microorganismes and Biomolécules Actives, Département de Biologie, Faculté des Sciences de Tunis, Campus Universitaire, 2092 Tunis, Tunisia.
authors.forename: Faten
authors.initials: F
authors.lastname: Ghodhbane-Gtari
authors.forename: Imen
authors.initials: I
authors.lastname: Nouioui
authors.forename: Mohamed
authors.initials: M
authors.lastname: Chair
authors.forename: Abdellatif
authors.initials: A
authors.lastname: Boudabous
authors.forename: Maher
authors.initials: M
authors.lastname: Gtari
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic; D040161:Frankia / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S