[Expressions of bacterial 16S rRNA, IL-1beta, TNF-alpha and NGF in prostate tissues].
OBJECTIVE
To investigate the role of bacteria in the etiology of chronic prostatitis.


METHODS
A total of 162 complete prostate specimens were obtained at autopsy from organ donors (aged 20 -38 yr) who died of non-prostatic diseases. Each of the samples from the peripheral zone of the prostate was divided into two parts, one for routine pathological examination and immunohistochemical studies of interleukin (IL)-1beta, tumor necrosis factor-alpha (TNF-alpha) and the nerve growth factor (NGF), and the other for PCR assay to detect the bacterial 16S rRNA gene (16S rDNA).


RESULTS
Fifty-one (31.5%) of the total specimens presented pathological changes of chronic prostatitis, of which 44 had mild focal stromal, 5 mild focal stromal and periglandular and 2 mild focal periglandular inflammation. The positive rate of 16S rDNA was 19.1% (31/162), 51.0% (26/51) in the chronic prostatitis and 4.5% (5/111) in the non-prostatitis specimens (chi2 = 29.783, P < 0.01). In the specimens with chronic prostatitis, the expressions of IL-1beta, TNF-alpha and NGF were significantly higher in the 16S rDNA positive than in the 16S rDNA negative group (P < 0.01).


CONCLUSIONS
Bacterial inflammation may play an important role in the etiology of chronic prostatitis.
Xie
Hui
H
Department of Urology, The First Hospital Affiliated to Wenzhou Medical College, Wenzhou, Zhejiang 325000, China. xiehuiwenzhou@yahoo.com.cn
Huang
Hui-Cong
HC
Yang
Yi-Rong
YR
He
Qiu-Xiang
QX
Zhu
Qi-Jian
QJ
Chen
Jian-Ou
JO
D000328:Adult; D002908:Chronic Disease; D020459:Genes, rRNA; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D020932:Nerve Growth Factor / Q000378:metabolism; D011467:Prostate / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D011472:Prostatitis / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D055815:Young Adult
D053583:Interleukin-1beta; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014409:Tumor Necrosis Factor-alpha; D020932:Nerve Growth Factor