Nosokomycins, new antibiotics discovered in an in vivo-mimic infection model using silkworm larvae. I: Fermentation, isolation and biological properties.
The in vivo-mimic assay system using silkworm larvae was used as a screening tool to discover antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). Microbial culture broths were screened in this in vivo-mimic assay system and a culture broth of Streptomyces sp. K04-0144 was selected. New antibiotics, designated nosokomycins A-D, were isolated from the culture broth by HP-20 and ODS column chromatography and HPLC. Nosokomycins inhibited the growth of MRSA with MIC values of 0.125 microg ml(-1) using the liquid microdilution method. Furthermore, MRSA-infected silkworms survived when nosokomycin A or B was injected at a dose of 50 microg per larva.
Uchida
Ryuji
R
Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Iwatsuki
Masato
M
Kim
Yong-Pil
YP
Ohte
Satoshi
S
Omura
Satoshi
S
Tomoda
Hiroshi
H
D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology*; D012831:Bombyx; D002240:Carbohydrate Sequence; D005285:Fermentation; D007814:Larva; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D013203:Staphylococcal Infections / Q000188:drug therapy*; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; D012717:Sesquiterpenes