A new norlupane triterpene from the leaves of Acanthopanax koreanum increases the differentiation of osteoblastic MC3T3-e1 cells.
A new norlupane-triterpene, 3alpha,11alpha-dihydroxy-20,23-dioxo-30-norlupane-28-oic acid (1), and four known compounds; 3alpha,11alpha-dihydroxy-23-oxo-lup-20(29)-en-28-oic acid (2), 3alpha,11alpha-dihydroxylup-20(29)-en-28-oic acid (3), ent-kaur-16-en-19-oic acid (4), and betulabuside B (5) were isolated from the methanolic extract of the leaves of Acanthopanax koreanum. Their chemical structures were elucidated by mass, 1D- and 2D-NMR spectroscopy. To investigate the bioactivities of these compounds, their effects on the differentiation of osteoblastic MC3T3-E1 cells were tested. Compounds 3 and 4 significantly increased osteoblastic cell growth and differentiation as assessed by the MTT assay and collagen content, respectively (p < 0.05). Compounds 1 and 5 significantly increased the growth of MC3T3-E1 cells and caused a significant elevation of osteoblastic cell differentiation as assessed by the alkaline phosphatase activity. These results suggest that the compounds isolated from A. koreanum leaves have a direct stimulatory effect on bone formation in vitro and may contribute to the prevention for osteoporosis.
Park
Sang Hyouk
SH
College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
Nhiem
Nguyen Xuan
NX
Kiem
Phan Van
PV
Choi
Eun Mi
EM
Kim
Jeong Ah
JA
Kim
Young Ho
YH
D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000378:metabolism; D028001:Eleutherococcus / Q000737:chemistry*; D004958:Estradiol / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D016339:Spectrometry, Mass, Fast Atom Bombardment; D017550:Spectroscopy, Fourier Transform Infrared; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology*
C549638:3,11-dihydroxy-20,23-dioxo-30-norlupane-28-oic acid; D010936:Plant Extracts; D014315:Triterpenes; D004958:Estradiol; D003094:Collagen; D000469:Alkaline Phosphatase