The glucocorticoid receptor in the distal nephron is not necessary for the development or maintenance of dexamethasone-induced hypertension.
Glucocorticoids are used as a treatment for a variety of conditions and hypertension is a well-recognized side effect of their use. The mechanism of glucocorticoid-induced hypertension is incompletely understood and has traditionally been attributed to promiscuous activation of the mineralocorticoid receptor by cortisol. Multiple lines of evidence, however, point to the glucocorticoid receptor as an important mediator as well. We have developed a mouse model of glucocorticoid-induced hypertension, which is dependent on the glucocorticoid receptor. To determine the site(s) of glucocorticoid receptor action relevant to the development of hypertension, we studied glucocorticoid-induced hypertension in a mouse with a tissue-specific knockout of the glucocorticoid receptor in the distal nephron. Although knockout mice had similar body weight, nephron number and renal histology compared to littermate controls, their baseline blood pressure was mildly elevated. Nevertheless, distal nephron glucocorticoid receptor knockout mice and controls had a similar hypertensive response to dexamethasone. Urinary excretion of electrolytes, both before and after administration of glucocorticoid was also indistinguishable between the two groups. We conclude that the glucocorticoid receptor in the distal nephron is not necessary for the development or maintenance of dexamethasone-induced hypertension in our model.
Goodwin
Julie E
JE
Section of Nephrology, Department of Pediatrics, Yale University School of Medicine, New Haven, CT, USA. julie.goodwin@yale.edu
Zhang
Junhui
J
Velazquez
Heino
H
Geller
David S
DS
D000818:Animals; D003907:Dexamethasone / Q000009:adverse effects*; D004573:Electrolytes / Q000652:urine; D005260:Female; D017353:Gene Deletion; D006973:Hypertension / Q000139:chemically induced* / Q000235:genetics*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009399:Nephrons / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D011965:Receptors, Glucocorticoid / Q000235:genetics*
D004573:Electrolytes; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone