Postantibiotic effect of purified melittin from honeybee (Apis mellifera) venom against Escherichia coli and Staphylococcus aureus.
Since the ancient times, the antibacterial application of honeybee venom (BV) has been practised and persisted. We investigated the antibacterial activity of whole BV and purified melittin against Escherichia coli and Staphylococcus aureus by the minimum inhibitory concentrations (MICs) and the postantibiotic effects (PAEs). The in vitro PAEs of whole BV and isolated melittin were determined using E. coli and S. aureus. The PAEs of whole BV against E. coli and S. aureus were 0.15 h (1 x MIC), 2.4 h (5 x MIC), and 3.45 h (1 x MIC), respectively. The PAEs of melittin against E. coli and S. aureus were 0.1 h (1 x MIC), 3.2 h (5 x MIC), and 4.35 h (1 x MIC), respectively. These results suggest that whole BV and melittin will be developed as a novel antibacterial drug.
Han
Sangmi
S
National Academy of Agricultural Science, Agricultural Biology, Suwon, South Korea. sangmih@rda.go.kr
Yeo
Joohong
J
Baek
Haju
H
Lin
Shin-Min
SM
Meyer
Stacy
S
Molan
Peter
P
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001516:Bees / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects*; D008555:Melitten / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects*; D014688:Venoms / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D014688:Venoms; D008555:Melitten