title: Involvement of nitric oxide in 5-HT(3) receptor agonist-induced fluid accumulation in jejunum and colon of anesthetized rats.
abstract: OBJECTIVE
  The aim of the present study was to investigate the involvement of nitric oxide in 5-HT(3) receptor agonist-induced fluid accumulation in jejunum and colon of anesthetized rats.
  
  
  METHODS
  Fluid movement in jejunum and colon were determined simultaneously in the same rat, by modifying the Beubler method. Nomega-nitro-L-arginine (L-NNA, 20 mg/kg, s.c) alone and in combination with L-arginine (L-Arg, 150 mg/kg s.c) or D-arginine (D-Arg, 150 mg/kg, s.c) were administered 30 min before administration of 1-PBG (18.5 mug/kg, i.v).
  
  
  RESULTS
  Intravenous administration of 1-phenylbiguanide (1-PBG) induced a net secretion of fluid in both jejunum and colon. 1-PBG had a more prominent secretory effect in the colon, causing a three-fold increase in volume of fluid secreted/g of colon than in the jejunum. Pretreatment with (L-NNA) prevented the 1-PBG-induced fluid accumulation in both jejunum and colon. The inhibitory effect of L-NNA on 1-PBG-induced fluid accumulation was reversed by L-Arg but not by D-Arg.
  
  
  CONCLUSIONS
  These results provide evidence that nitric oxide plays an important role in 5-HT(3) receptor agonist-induced fluid accumulation in jejunum and colon of anesthetized rats.
authors.affiliation: Department of Pharmacology, K.L.E.Ss College of Pharmacy, J.N.M.C. Campus, Nehru Nagar, Belgaum - 590 010, India.
authors.forename: B
authors.initials: B
authors.lastname: Veeresh
authors.forename: Basanagouda M
authors.initials: BM
authors.lastname: Patil
authors.forename: S V
authors.initials: SV
authors.lastname: Veeresh Babu
authors.forename: Neelakanth M
authors.initials: NM
authors.lastname: Jeedi
authors.forename: Banappa S
authors.initials: BS
authors.lastname: Unger