title: Evaluating binuclear copper(II) complexes for glycoside hydrolysis.
abstract: Three binuclear copper(II) complexes were characterized as solids by X-ray diffraction and in solution by UV/vis spectrophotometric titration, and subsequently evaluated for their glycosidase-like activity. The structure analysis revealed comparable intermetallic Cu...Cu distances (approximately 3.5 A) for the complexes 2 and 3. Despite this similarity, the composition of the complexes differs significantly in aqueous solution as revealed by spectrophotometric titrations. The hydrolysis of selected nitrophenylglycopyranosides is up to 11,000-fold accelerated over background in the presence of the copper(II) complexes in 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS) buffer at pH 10.5 and 30 degrees C.
authors.affiliation: Department of Chemistry and Biochemistry, 179 Chemistry Building, Auburn University, Auburn, Alabama 36849, USA. susanne.striegler@auburn.edu
authors.forename: Susanne
authors.initials: S
authors.lastname: Striegler
authors.forename: Natasha A
authors.initials: NA
authors.lastname: Dunaway
authors.forename: Moses G
authors.initials: MG
authors.lastname: Gichinga
authors.forename: James D
authors.initials: JD
authors.lastname: Barnett
authors.forename: Anna-Gay D
authors.initials: AG
authors.lastname: Nelson
mesh_terms: D002384:Catalysis; D003300:Copper / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008958:Models, Molecular; D015394:Molecular Structure; D013055:Spectrophotometry, Infrared; D014961:X-Ray Diffraction
chemical_list: D006027:Glycosides; D003300:Copper