title: In situ study of tetrachloroethylene bioremediation with different microbial community shifting.
abstract: In this study, we characterized the microbial community in groundwater contaminated with tetrachloroethylene (PCE) in order to evaluate the intrinsic and enhanced bioremediation of PCE. Variable behaviour of microbes was observed between natural attenuation and biostimulation, where the latter was mediated by the addition of nutrients. Results of denaturing gradient gel electrophoresis (DGGE) of amplified bacterial 16S rDNA in the case of biostimulation showed that the microbial community was dominated by species phylogenetically related to the beta-proteobacteria. With regards to natural attenuation, sequences were found belonging to multiple species of different phyla. Interestingly, we found sequences that matched the species belonging to the Firmicutes, which contains bacteria capable of reductive dehalogenation. These results suggest the possibility of the presence of some Clostridium-like PCE degraders within the microbial community when using bioremediation or biostimulation.
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, Gifu, Japan.
authors.forename: Arpita
authors.initials: A
authors.lastname: Bhowmik
authors.forename: Akane
authors.initials: A
authors.lastname: Asahino
authors.forename: Takanori
authors.initials: T
authors.lastname: Shiraki
authors.forename: Kohei
authors.initials: K
authors.lastname: Nakamura
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takamizawa
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D013750:Tetrachloroethylene / Q000493:pharmacokinetics*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics*; D014876:Water Pollution / Q000517:prevention & control*
chemical_list: D014874:Water Pollutants, Chemical; D013750:Tetrachloroethylene