Embryo development and gestation using fresh and vitrified oocytes.
BACKGROUND
The objective of this study was to compare the gestational results obtained with vitrified/thawed oocytes by a novel vitrification method (Vitri-ingá) to results obtained with fresh oocytes.


METHODS
A total of 125 IVF-ET procedures carried out over 2008 were analysed, in which 79 patients received embryos from fresh oocytes (Group 1), and 46 patients received embryos from vitrified/thawed oocytes using Vitri-ingá (Group 2). Fresh and vitrified/thawed oocytes were fertilized and embryos were transferred. Fertilization, pregnancy and implantation rates were compared.


RESULTS
Vitrified oocytes presented a survival rate of 84.9%. Fertilization, pregnancy and implantation rates showed no statistically significant differences between fresh and cryopreserved/thawed groups (81.3, 51.9, 21.3% and 80.8, 45.6 and 14.9%, respectively). Only the average number of blastomeres in Group 1 (6.86 +/- 2.07) was significantly higher than in Group 2 (6.35 +/- 2.26; P = 0.010).


CONCLUSIONS
Despite some differences in the patient groups, the clinical results of this study demonstrated that the Vitri-ingá method preserves the potential of human vitrified oocytes for fertilization and further development.
Almodin
Carlos Gilberto
CG
Materbaby Human Reproduction-Human Reproduction, Av XV de Novembro, 1232, Maringá, Paraná 87013230, Brazil. almodin@materbaby.com.br
Minguetti-Camara
Vania Cibele
VC
Paixao
Cassia Lopes
CL
Pereira
Priscila Cardoso
PC
D000328:Adult; D001757:Blastomeres / Q000166:cytology; D002470:Cell Survival; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D010064:Embryo Implantation; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D009865:Oocytes / Q000166:cytology*; D011247:Pregnancy