Expression of fibroblast growth factor receptors 1-4 in human chronic tympanic membrane perforation.
OBJECTIVE
To locate fibroblast growth factor receptor (FGFRs) 1-4 in human chronic tympanic membrane (TM) perforation.


METHODS
A sample of human chronic TM perforation was harvested during myringoplasty. The sample was immediately fixed in 4% paraformaldehyde and embedded in OCT compound. Immunohistochemistry was performed with FGFR 1-4 polyclonal antibodies.


RESULTS
FGFRs 1-4 were strongly and weakly expressed in the epidermal and mucosal layer of the TM perforation, respectively.


CONCLUSIONS
As it is impossible to perform quantitative analysis based on the fluorescence intensity of each immunoreactivity, the presence of FGFRs 1-4 in the human chronic TM perforation is shown. The expressions of FGFRs 1-4 indicated that the clinical use of bFGF agent is useful for myringoplasty.
Kakigi
Akinobu
A
Department of Otolaryngology, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan. kakigi-tky@umin.ac.jp
Uchida
Aya
A
Nishimura
Masahiko
M
Takeda
Taizo
T
Takeda
Setsuko
S
Nakatani
Hiroaki
H
D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D009225:Myringoplasty; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000378:metabolism*; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000378:metabolism*; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000378:metabolism*; D051499:Receptor, Fibroblast Growth Factor, Type 4 / Q000378:metabolism*; D018058:Tympanic Membrane Perforation / Q000378:metabolism* / Q000601:surgery
D051496:Receptor, Fibroblast Growth Factor, Type 1; D051497:Receptor, Fibroblast Growth Factor, Type 2; D051498:Receptor, Fibroblast Growth Factor, Type 3; D051499:Receptor, Fibroblast Growth Factor, Type 4