85-kDa protein of Trypanosoma cruzi purified by affinity chromatography used in the multiple antigen binding assay (MABA) for the diagnosis of T. cruzi infection in a Venezuelan rural community.
No ideal test exists for Chagas' disease, and better diagnostic strategies are needed. We determined the diagnostic utility of an 85-kDa Trypanosoma cruzi protein in a multiple antigen binding assay (MABA). A standardized MABA test based on concentrated trypomastigote excretory-secretory antigen (TESA) and an 85-kDa purified protein showed 100% sensitivity and specificity. In field conditions, 6/66 individuals tested in a region not thought to be endemic (Rio Brito) were identified as seropositive for T. cruzi infection with our MABA test. In parallel, an enzyme-linked immunosorbent assay based on fixed epimastigotes detected 7/66 positives, which were independently confirmed. These data suggest that the 85-kDa and TESA proteins could be used in the MABA format as a complementary tool for the diagnosis of latent Chagas' disease. High anti-T. cruzi antibody detection rates, poor knowledge of Chagas' disease and its vector, and the demonstration of infected vectors in the study community all suggest a significant risk of reemergence of T. cruzi infection in this region of Venezuela.
Berrizbeitia
Mariolga
M
Postgrado en Biología Aplicada, Núcleo de Sucre, Universidad de Oriente, Cumaná, Venezuela.
Ward
Brian J
BJ
Bubis
José
J
Gottschalk
Marcelo
M
Aché
Alberto
A
Perdomo
Deisy
D
Medina
Rafael
R
Medina
Mehudy
M
Spencer
Lilian
L
Ndao
Momar
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000953:Antigens, Protozoan / Q000302:isolation & purification; D014355:Chagas Disease / Q000175:diagnosis* / Q000469:parasitology; D002648:Child; D002846:Chromatography, Affinity; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male; D008562:Membrane Glycoproteins / Q000302:isolation & purification; D008875:Middle Aged; D015800:Protozoan Proteins / Q000302:isolation & purification; D012424:Rural Population; D012680:Sensitivity and Specificity; D014349:Trypanosoma cruzi / Q000276:immunology / Q000302:isolation & purification*; D014687:Venezuela; D055815:Young Adult
D000953:Antigens, Protozoan; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; C096427:SA85 1.1 antigen, Trypanosoma cruzi