Stimulation of cephalosporin C production in Acremonium chrysogenum M35 by glycerol.
In this study, the effects of glycerol on cephalosporin C production by Acremonium chrysogenum M35 were evaluated. The addition of glycerol increased cephalosporin production by up to 12-fold. Glycerol caused the upregulation of the transcription of the isopenicillin synthase (pcbC) and transporter (cefT) genes in early exponential phase, and affected the cell morphology since hyphal fragments differentiated into arthrospores. These results indicate that glycerol effectively enhances cephalosporin C production via stimulation of cell differentiation.
Shin
Hyun Yong
HY
Department of Chemical Biological Engineering, Korea University, 1-5Ka, Anam-Dong, Sungbuk-Ku, Seoul 136-701, Republic of Korea.
Lee
Jin Young
JY
Jung
You Ree
YR
Kim
Seung Wook
SW
D000164:Acremonium / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D002244:Carbon / Q000494:pharmacology; D002511:Cephalosporins / Q000096:biosynthesis*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D002511:Cephalosporins; D012333:RNA, Messenger; C025163:cephalosporin C; D002244:Carbon; D005947:Glucose; D005990:Glycerol