mRNA expression of the XAGE-1 gene in human acute leukemia.
XAGE-1 is a cancer-testis antigen, which was demonstrated to be expressed at a significant frequency and to be immunogenic in some solid tumors. We analyzed the expression of 4 XAGE-1 transcript variant gene in human acute leukemias by reverse-transcription polymerase chain reaction. Among the 114 acute leukemias, 14/63 (22.22%) of the acute myeloid leukemia samples were positive for XAGE-1b genes. XAGE-1b mRNA expression was detected in 10/51 (19.61%) of the acute lymphocyte leukemia samples. However, we did not find any important correlation between XAGE-1b mRNA expression and clinical characteristics, such as sex, leukemia type, response to therapy and the percentage of blast in the first diagnosed bone marrow. We concluded that the XAGE-1b gene was expressed at the mRNA level in a proportion of human acute leukemia.
Ji
Yuqiang
Y
Department of Clinical Hematology, Second Hospital of Xi'an Jiaotong University, 157# West 5th Road, Xi'an, Shanxi, China.
Zhang
Wanggang
W
Wang
Jin
J
Gu
Liufang
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000235:genetics*; D001853:Bone Marrow / Q000502:physiology; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D000951:Antigens, Neoplasm; D012333:RNA, Messenger; C413656:XAGE1A protein, human