title: Genetic variation of G4P[6] rotaviruses: evidence for novel strains circulating between the hospital and community.
abstract: One hundred forty-six fecal specimens collected between 2007 and 2008 from infants with acute gastroenteritis were screened for rotavirus by ELISA with VP6-specific antibody. One hundred twenty-three of the samples (84.2%) were confirmed to be positive for group A rotavirus (community-acquired, n = 90 [73.2%] and nosocomial, n = 33 [26.8%]), and were typed subsequently using RT-PCR and sequence analysis methods. Determination of G- and P-type combinations showed that G4P[6] (78.9%) was the most common strain, followed by G3P[8] (7.3%), G1P[8] (6.5%), G2P[4] (0.8%), G2P[6] (0.8%), G1P[6] (0.8%), and G9P[8] (0.8%) strains. Of the 97 G4P[6] strains, 62 (63.8%) were responsible for community-acquired cases and 35 (36.1%) were hospital-acquired cases. Phylogenetic analysis of the VP7 gene from the G4P[6] strains revealed that both the community-acquired and nosocomial strains were segregated to the human rotaviruses circulating world-wide, including the prototype vaccinal strain, ST3, which constituted a novel sublineage in lineage 1. Owing to the recent emergence of G4P[6] rotaviruses within the hospital, as well as in the community, the findings from this study are important since they provide new information concerning the community and nosocomial spread of rotaviruses.
authors.affiliation: Department of Pediatrics, Gachon University Gil Hospital, Incheon, South Korea.
authors.forename: So-Yeon
authors.initials: SY
authors.lastname: Shim
authors.forename: Yeon-Chang
authors.initials: YC
authors.lastname: Jung
authors.forename: Van Phan
authors.initials: VP
authors.lastname: Le
authors.forename: Dong Woo
authors.initials: DW
authors.lastname: Son
authors.forename: Eell
authors.initials: E
authors.lastname: Ryoo
authors.forename: Jung Ok
authors.initials: JO
authors.lastname: Shim
authors.forename: Inseok
authors.initials: I
authors.lastname: Lim
authors.forename: Wonyong
authors.initials: W
authors.lastname: Kim
mesh_terms: D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D016000:Cluster Analysis; D017714:Community-Acquired Infections / Q000821:virology; D003428:Cross Infection / Q000821:virology; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012703:Serotyping
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C050779:VP7 protein, Rotavirus