title: Gene expression profiles of adipose tissue of high-fat diet-induced obese rats by cDNA microarrays.
abstract: To better understand the molecular basis of dietary obesity, we examined adipose tissue genes differentially expressed in a well-characterized rat model of high-fat diet (HFD)-induced obesity using cDNA microarrays. Male Sprague-Dawley rats were fed either the HFD or the normal diet. Seven weeks later, the weights of obese models (362.92 ± 39.65 g) were significantly higher than those of normal control rats (315.22 ± 42.30 g, P < 0.01) and the wet weights of adipose tissue of rats fed with HFD (9.29 ± 5.14 g) were significantly higher than those of normal control rats (4.09 ± 2.69 g, P < 0.01), which confirmed the successful preparation of obese models. cDNA microarrays containing 9 216 genes/Ests were used to investigate gene expression of adipose tissue. Autoradiographic analysis showed that 532, 154, and 22 genes were differently expressed over 2-, 3-, and 5-fold, respectively. The analysis of gene expression profiles indicated that 276 genes were up-regulated and 432 genes were down-regulated in response to HFD-induced obesity. Different clusters of genes associated with lipid metabolism, extracellular matrix, signal transduction, cytoskeleton, cell apoptosis, etc., such as VLCS-H2, DGAT, ACADVL, PHYH, SCD, ACACA, ACS, MMP-2, MMP-15, CD38, CAMK2D, CACNA1F, CAPZA2, TMOD3, ARPC2, KNS2, TPM1, MAPK8, GADD45B, DAXX, TOK-1, PRKACA, STAT6, were concerned.
authors.affiliation: Department of Newborn Infants, Nanjing Children's Hospital of Nanjing Medical University, 210008, Nanjing, China. jieqiu@yahoo.cn
authors.forename: Jie
authors.initials: J
authors.lastname: Qiu
authors.forename: Rui
authors.initials: R
authors.lastname: Cheng
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Zhou
authors.forename: Jin-gai
authors.initials: JG
authors.lastname: Zhu
authors.forename: Chun
authors.initials: C
authors.lastname: Zhu
authors.forename: Da-ni
authors.initials: DN
authors.lastname: Qin
authors.forename: Chun-zhao
authors.initials: CZ
authors.lastname: Kou
authors.forename: Xi-rong
authors.initials: XR
authors.lastname: Guo
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D004041:Dietary Fats; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008297:Male; D009765:Obesity / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004041:Dietary Fats