title: Localization and imaging of sialylated glycosphingolipids in brain tissue sections by MALDI mass spectrometry.
abstract: In this study, we describe a simple and efficient method for mapping the distribution and localization of all sialylated sphingoglycolipids present in coronal mouse brain sections using a conventional axial matrix-assisted laser desorption/ionization time of flight. A single scan of a histological tissue section gives a complete profile of ganglioside species without derivatization or labeling. We have developed and tested a new matrix preparation (2,6-dihydroxyacetophenone [DHA]/ammonium sulfate/heptafluorobutyric acid [HFBA]) to maximize the detection of all ganglioside species; the ammonium sulfate limits the formation of salt adducts, while the addition of HFBA increases the stability of DHA in a vacuum, thus facilitating imaging applications. Our results, in both extracted samples and whole tissue sections using negative ion reflectron and linear modes, show differences in localization in several brain regions depending on the sialic acids and the ceramide-associated core gangliosides.
authors.affiliation: Cellular Neurobiology, NIDA IRP, NIH, Baltimore, MD 21224, USA.
authors.forename: Benoit
authors.initials: B
authors.lastname: Colsch
authors.forename: Amina S
authors.initials: AS
authors.lastname: Woods
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D001923:Brain Chemistry; D006028:Glycosphingolipids / Q000032:analysis* / Q000737:chemistry* / Q000493:pharmacokinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D012794:Sialic Acids / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016707:Tissue Fixation
chemical_list: D006028:Glycosphingolipids; D012794:Sialic Acids