title: TBCCD1, a new centrosomal protein, is required for centrosome and Golgi apparatus positioning.
abstract: In animal cells the centrosome is positioned at the cell centre in close association with the nucleus. The mechanisms responsible for this are not completely understood. Here, we report the first characterization of human TBCC-domain containing 1 (TBCCD1), a protein related to tubulin cofactor C. TBCCD1 localizes at the centrosome and at the spindle midzone, midbody and basal bodies of primary and motile cilia. Knockdown of TBCCD1 in RPE-1 cells caused the dissociation of the centrosome from the nucleus and disorganization of the Golgi apparatus. TBCCD1-depleted cells are larger, less efficient in primary cilia assembly and their migration is slower in wound-healing assays. However, the major microtubule-nucleating activity of the centrosome is not affected by TBCCD1 silencing. We propose that TBCCD1 is a key regulator of centrosome positioning and consequently of internal cell organization.
authors.affiliation: Instituto Gulbenkian de Ciência, Oeiras, Portugal.
authors.forename: João
authors.initials: J
authors.lastname: Gonçalves
authors.forename: Sofia
authors.initials: S
authors.lastname: Nolasco
authors.forename: Rute
authors.initials: R
authors.lastname: Nascimento
authors.forename: Mónica
authors.initials: M
authors.lastname: Lopez Fanarraga
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Zabala
authors.forename: Helena
authors.initials: H
authors.lastname: Soares
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism; D018385:Centrosome / Q000648:ultrastructure*; D002923:Cilia / Q000378:metabolism; D020868:Gene Silencing; D005816:Genetic Complementation Test; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D018832:Molecular Chaperones / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D008941:Spindle Apparatus
chemical_list: D002352:Carrier Proteins; D018832:Molecular Chaperones; C551234:TBCCD1 protein, human; C455270:tubulin-specific chaperone C