Simultaneous suppression of three genes related to brassinosteroid (BR) biosynthesis altered campesterol and BR contents, and led to a dwarf phenotype in Arabidopsis thaliana.
We generated transgenic lines of Arabidopsis thaliana with an RNA interference construct that expressed hairpin double-stranded RNA for DET2:DWF4:SMT2 to induce sequence-specific RNA silencing. In transgenic plants, expressions of DET2, DWF4, and SMT2 were simultaneously reduced, and the campesterol content was increased by up to 420% compared to the level in the wild-type plant. Triple knock-down of the DET2, DWF4, and SMT2 enzymes also resulted in reduction of brassinosteroid (BR)-specific biosynthesis intermediates. Transgenic plants harboring the RNA interference construct displayed a semi-dwarf phenotype due to altered development. Our findings indicate that redesigning of plant architecture is possible through simultaneous suppression of multiple genes involved in BR biosynthesis.
Chung
Ho Yong
HY
Department of Genetic Engineering, Plant Metabolism Research Center, Kyung Hee University, Yongin, 446-701, Korea.
Fujioka
Shozo
S
Choe
Sunghwa
S
Lee
Soyoun
S
Lee
Youn Hyung
YH
Baek
Nam In
NI
Chung
In Sik
IS
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000096:biosynthesis; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D017343:Genes, Plant; D008780:Methyltransferases / Q000235:genetics; D010641:Phenotype; D010840:Phytosterols / Q000096:biosynthesis*; D010937:Plant Growth Regulators / Q000096:biosynthesis*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013256:Steroids / Q000096:biosynthesis*
C111451:AT3G50660 protein, Arabidopsis; D029681:Arabidopsis Proteins; C098907:DET2 protein, Arabidopsis; D018076:DNA, Complementary; D010840:Phytosterols; D010937:Plant Growth Regulators; D012330:RNA, Double-Stranded; D018749:RNA, Plant; D013256:Steroids; C021273:campesterol; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D008780:Methyltransferases; C481389:sterol methyltransferase 2, Arabidopsis