Sperm morphology, motility and fertilisation capacity in the myobatrachid frog Crinia georgiana.
Sperm traits have been found to vary between individuals within populations in a variety of taxa. Sperm motility, morphometry and viability may be expected to have important effects on male fertility, although previous studies have found varying patterns, especially in external fertilisers. In the present study, we examined the effects of sperm swimming velocity, the proportion of motile spermatozoa, sperm head and tail length and the proportion of live spermatozoa on fertilisation success in the externally fertilising myobatrachid frog Crinia georgiana using IVF techniques and by controlling sperm numbers. We found no effect of any of the sperm traits we measured on IVF success. Neither did we find any relationship between sperm morphology and sperm performance. There was a negative relationship between sperm viability and male body size, which could be a function of age or an alternative tactic of differential investment in spermatozoa by smaller-sized males using sneak tactics in multiple matings. In contrast with most externally fertilising aquatic organisms, high rates of fertilisation appear to be achieved in C. georgiana with relatively low sperm swimming speeds.
Dziminski
Martin A
MA
Centre for Evolutionary Biology, School of Animal Biology (M092), The University of Western Australia, Crawley, WA 6009, Australia.
Roberts
J D
JD
Simmons
Leigh W
LW
D000818:Animals; D001001:Anura / Q000502:physiology*; D001835:Body Weight / Q000502:physiology; D048430:Cell Shape; D005298:Fertility / Q000502:physiology; D005306:Fertilization / Q000502:physiology; D008297:Male; D009929:Organ Size / Q000502:physiology; D013076:Sperm Count; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology*; D013737:Testis / Q000502:physiology