PIM-1 gene RNA interference induces growth inhibition and apoptosis of prostate cancer cells and suppresses tumor progression in vivo.
BACKGROUND
The goal of this study was to investigate the roles of PIM-1 in prostate cancer (CaP) cell proliferation and apoptosis, and to assess the potential of PIM-1 as a target for CaP therapy.


METHODS
Using RNAi technology, we knocked down the expression of PIM-1 in PC-3 cell. After siRNA transfection, cell morphology, cell proliferation, cell cycle, and apoptosis rate were analyzed. PIM-1 siRNA with Lipofectamine were injected into xenograft models to evaluate its therapeutic effect.


RESULTS
PIM-1 siRNA significantly inhibited PIM-1 expression. In vitro, silencing of the PIM-1 gene resulted in irregular cell morphology, decreased cell proliferation, inhibition of cell-cycle progression, and induction of apoptosis. Compared with control groups, intratumoral injection of PIM-1 siRNA with Lipofectamine in nude mice dramatically suppressed PC-3 tumor progression.


CONCLUSIONS
PIM-1 could play important roles in the progression of CaP and may be an interesting target for CaP therapy.
Zhang
Tong
T
Tianjin Institute of Urology, Second Hospital of Tianjin Medical University, Tianjin, China.
Zhang
Xiaoguang
X
Ding
Kejia
K
Yang
Kuo
K
Zhang
Zhihong
Z
Xu
Yong
Y
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology*; D045744:Cell Line, Tumor; D020868:Gene Silencing; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D034741:RNA, Small Interfering