title: Regulation of the microRNA processor DGCR8 by the tumor suppressor ING1.
abstract: The ING family of tumor suppressor proteins controls several cellular functions relevant to antitumor protection, such as cell cycle control, apoptosis, senescence, or migration. ING proteins are functionally linked to the p53 pathway, and they participate in transcriptional control via the recognition of histone marks and recruitment of protein complexes with chromatin-modifying activity to specific promoters. Here, we have investigated the global effect of ING1 in gene regulation through genome-wide analysis of expression profiles in primary embryonic fibroblasts deficient for the Ing1 locus. We find that Ing1 has a predominant role as transcriptional repressor in this setting, affecting the expression of genes involved in a variety of cellular functions. Within the subset of genes showing differential expression, we have identified DGCR8, a protein involved in the early steps of microRNA biogenesis. We show that ING1 binds to the DGCR8 promoter and controls its transcription through chromatin regulation. We also find that ING1 and DGCR8 can cooperate in restraining proliferation. In summary, this study reveals a novel connection between ING1 and a regulator of microRNA biogenesis and identifies new links between tumor suppressor proteins and the microRNA machinery.
authors.affiliation: Instituto de Investigaciones Biomédicas CSIC-UAM, Madrid, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gómez-Cabello
authors.forename: Sergio
authors.initials: S
authors.lastname: Callejas
authors.forename: Alberto
authors.initials: A
authors.lastname: Benguría
authors.forename: Alberto
authors.initials: A
authors.lastname: Moreno
authors.forename: Javier
authors.initials: J
authors.lastname: Alonso
authors.forename: Ignacio
authors.initials: I
authors.lastname: Palmero
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016147:Genes, Tumor Suppressor; D006801:Humans; D000076023:Inhibitor of Growth Protein 1; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D016601:RNA-Binding Proteins; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C474044:Dgcr8 protein, mouse; C501505:Ing1 protein, mouse; D000076023:Inhibitor of Growth Protein 1; D047908:Intracellular Signaling Peptides and Proteins; D035683:MicroRNAs; D009687:Nuclear Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; D025521:Tumor Suppressor Proteins