High-mobility group box-1 protein in tracheal aspirates from premature infants: relationship with bronchopulmonary dysplasia and steroid therapy.
OBJECTIVE
High-mobility group box-1 (HMGB1) is a potent inflammatory mediator and contributes to acute lung injury in adults. The role of HMGB1 in neonatal lung injury and the development of bronchopulmonary dysplasia (BPD) is unknown. We studied the association between HMGB1 levels in tracheal aspirates (TAs) and adverse outcomes (BPD/death) in ventilated premature infants (VPIs) and modulation of HMGB1 levels with dexamethasone (Dex) use.


METHODS
Infants born before 32 weeks gestation and requiring mechanical ventilation were enrolled. Serial TA samples were collected on days 1, 3, 5 and 7 and HMGB1 levels were measured. HMGB1 levels in TA samples were compared between infants with no BPD and infants who developed BPD or died. HMGB1 TA levels were also compared before and after using Dex.


RESULTS
In all, 24 infants (gestational age 26.4+/-1.9 weeks, birth weight 859+/-200 g) had no BPD, 60 infants (gestational age 25.4+/-1.8 weeks, birth weight 749+/-156 g) developed BPD or died before 36 weeks postmenstrual age. Mean HMGB1 level in first week of life was significantly lower in infants with no BPD (27.3+/-16.5 ng mg(-1)) compared with those who developed BPD or died (45.1+/-30.9 ng mg(-1), P=0.004). In total, 29 VPIs received Dex. There was no significant change in HMGB1 levels with steroid therapy (before 47.0+/-43.9, after 60.1.5+/-58.8, P=0.3).


CONCLUSIONS
Our data suggest that higher HMGB1 levels in TA are associated with the development of BPD or death in VPI. Dex use had no effect on HMGB1 levels.
Aghai
Z H
ZH
Department of Pediatrics, Cooper University Hospital, Camden, NJ, USA. Aghai-zubair@cooperhealth.edu
Saslow
J G
JG
Meniru
C
C
Porter
C
C
Eydelman
R
R
Bhat
V
V
Stahl
G
G
Sannoh
S
S
Pyon
K
K
Hewitt
C
C
Bhandari
V
V
D001997:Bronchopulmonary Dysplasia / Q000188:drug therapy / Q000378:metabolism* / Q000401:mortality; D003907:Dexamethasone / Q000627:therapeutic use; D005260:Female; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D052577:Infant, Extremely Low Birth Weight; D007231:Infant, Newborn; D007234:Infant, Premature; D008297:Male; D012121:Respiration, Artificial / Q000009:adverse effects; D013183:Sputum / Q000378:metabolism*; D013256:Steroids / Q000627:therapeutic use; D014132:Trachea
C512050:HMBOX1 protein, human; D018398:Homeodomain Proteins; D013256:Steroids; D003907:Dexamethasone