title: Microfluidic Reactor Array Device for Massively Parallel In-situ Synthesis of Oligonucleotides.
abstract: We have designed and fabricated a microfluidic reactor array device for massively parallel in-situ synthesis of oligonucleotides (oDNA). The device is made of glass anodically bonded to silicon consisting of three level features: microreactors, microchannels and through inlet/outlet holes. Main challenges in the design of this device include preventing diffusion of photogenerated reagents upon activation and achieving uniform reagent flow through thousands of parallel reactors. The device embodies a simple and effective dynamic isolation mechanism which prevents the intermixing of active reagents between discrete microreactors. Depending on the design parameters, it is possible to achieve uniform flow and synthesis reaction in all of the reactors by proper design of the microreactors and the microchannels. We demonstrated the use of this device on a solution-based, light-directed parallel in-situ oDNA synthesis. We were able to synthesize long oDNA, up to 120 mers at stepwise yield of 98 %. The quality of our microfluidic oDNA microarray including sensitivity, signal noise, specificity, spot variation and accuracy was characterized. Our microfluidic reactor array devices show a great potential for genomics and proteomics researches.
authors.affiliation: Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Onnop
authors.initials: O
authors.lastname: Srivannavit
authors.forename: Mayurachat
authors.initials: M
authors.lastname: Gulari
authors.forename: Zhishan
authors.initials: Z
authors.lastname: Hua
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Gao
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Ailing
authors.initials: A
authors.lastname: Hong
authors.forename: Tiecheng
authors.initials: T
authors.lastname: Zhou
authors.forename: Erdogan
authors.initials: E
authors.lastname: Gulari