title: Expression and immunolocalization of calcium transport proteins in the canine duodenum, kidney, and pancreas.
abstract: The aim of the study was to elucidate expression and localization of several calcium handling proteins and the regulating vitamin D receptor (VDR) in the dog using RT-PCR and immunohistochemistry. The results of RNA expression and antibody staining were compared to ensure specificity. We found several antibodies to be useful for further studies on calcium homeostasis.
authors.affiliation: Clinic of Small Animal Medicine, LMU University of Munich, Muenchen, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Palm
authors.forename: Katrin
authors.initials: K
authors.lastname: Hartmann
authors.forename: Karin
authors.initials: K
authors.lastname: Weber
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D064026:Calbindins; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D004386:Duodenum / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064030:S100 Calcium Binding Protein G / Q000235:genetics / Q000378:metabolism; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism; D019831:Sodium-Calcium Exchanger / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism
chemical_list: D000906:Antibodies; D064026:Calbindins; D015220:Calcium Channels; D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D064030:S100 Calcium Binding Protein G; D019831:Sodium-Calcium Exchanger; D050916:TRPV Cation Channels; C120776:TRPV6 channel; C110374:sodium-calcium exchanger 1; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases; D002118:Calcium