Determination of angiotensin-I converting enzyme inhibitory peptides in chicken leg bone protein hydrolysate with alcalase.
This study aims to identify peptides with angiotensin-I converting enzyme (ACE) inhibitory activity in hydrolysate from chicken leg bone protein hydrolyzed with alcalase for 4 h (A4H). The hydrolysate has demonstrated potent in vitro ACE inhibitory activity, and has been shown to attenuate the development of hypertension and cardiovascular hypertrophy in spontaneously hypertensive rats (SHR). A4H is competitive for ACE and was separated using high-performance liquid chromatography (HPLC) with a gel filtration column (Superdex Peptide HR 10/30). The results show that A4H is a mixed non-competitive inhibitor. Eighteen fractions were detected after separation of A4H, and most of them showed ACE inhibitory activity. Five fractions with strong ACE inhibitory activities (above 50%) were labeled from A to E. In addition, there were 10 peptides, consisting of 5-10 amino acid residues that were identified from fraction D that exhibited the strongest ACE inhibitory activity. Three of the identified peptides corresponded to peptides derived from collagen type I and chicken muscular protein. It is revealed that A4H has several peptides that possess ACE inhibitory activities.
Cheng
Fu-Yuan
FY
Department of Hospitality Management, Toko University, Chiayi City, Taiwan.
Wan
Tien-Chun
TC
Liu
Yu-Tse
YT
Chen
Chi-Ming
CM
Lin
Liang-Chuan
LC
Sakata
Ryoichi
R
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D002645:Chickens; D024042:Collagen Type I / Q000737:chemistry; D006868:Hydrolysis; D006973:Hypertension / Q000188:drug therapy / Q000378:metabolism; D007867:Leg Bones / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D013381:Subtilisins / Q000378:metabolism*
D000806:Angiotensin-Converting Enzyme Inhibitors; D024042:Collagen Type I; D010446:Peptide Fragments; D011506:Proteins; D007703:Peptidyl-Dipeptidase A; D013381:Subtilisins