title: Drastic changes in fecal and mucosa-associated microbiota in adult patients with short bowel syndrome.
abstract: Short bowel syndrome (SBS) is observed in Humans after a large resection of gut. Since the remnant colon and its associated microbiota play a major role in the outcome of patients with SBS, we studied the overall qualitative and quantitative microbiota composition of SBS adult patients compared to controls. The population was composed of 11 SBS type II patients (with a jejuno-colonic anastomosis) and 8 controls without intestinal pathology. SBS patients had 38 +/- 30 cm remnant small bowel length and 66 +/- 19% of residual colon. The repartition of proteins, lipids, carbohydrates and fibres was expressed as % of total oral intake in patients and controls. The microbiota was profiled from stool and biopsy samples with temporal temperature gradient gel electrophoresis and quantitative PCR. We show here that microbiota of SBS patients is unbalanced with a high prevalence of Lactobacillus along with a sub-dominant presence and poor diversity of Clostridium leptum, Clostridium coccoides and Bacteroidetes. In addition, Lactobacillus mucosae was detected within the fecal and mucosa-associated microbiota of SBS patients, whereas it remained undetectable in controls. Thus, in SBS the microbial composition was deeply altered in fecal and mucosal samples, with a shift between dominant and sub-dominant microbial groups and the prevalence of L. mucosae.
authors.affiliation: Service de Gastroentérologie et Assistance Nutritive, Centre de Transplantation Intestinale, Pôle des Maladies de l'Appareil Digestif, Hôpital Beaujon, Clichy, Université Paris 7, France. francisca.joly@bjn.aphp.fr
authors.forename: Francisca
authors.initials: F
authors.lastname: Joly
authors.forename: Camille
authors.initials: C
authors.lastname: Mayeur
authors.forename: Aurélia
authors.initials: A
authors.lastname: Bruneau
authors.forename: Marie-Louise
authors.initials: ML
authors.lastname: Noordine
authors.forename: Thierry
authors.initials: T
authors.lastname: Meylheuc
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.forename: Bernard
authors.initials: B
authors.lastname: Messing
authors.forename: Pierre-Henri
authors.initials: PH
authors.lastname: Duée
authors.forename: Claire
authors.initials: C
authors.lastname: Cherbuy
authors.forename: Muriel
authors.initials: M
authors.lastname: Thomas
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003013:Clostridium / Q000235:genetics / Q000302:isolation & purification; D015331:Cohort Studies; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification; D054892:Metagenome; D008875:Middle Aged; D009752:Nutritional Status; D012778:Short Bowel Syndrome / Q000382:microbiology* / Q000473:pathology / Q000503:physiopathology