title: Dose-dependent effect of adipose-derived adult stem cells on vertical bone regeneration in rabbit calvarium.
abstract: Previous in vivo studies have shown a limited potential for vertical bone regeneration using osteoconductive scaffolds alone. In the present study, we investigated whether the association of adipose-derived adult stem cells (ASCs) with anorganic bovine bone (ABB) scaffold improved bone formation and implant osseointegration in a vertical guided bone regeneration model. Two pre-formed titanium domes were placed on the calvaria of 12 rabbits. Four treatment modalities were evenly distributed among the 24 domes: ABB alone, and ABB containing 3 x 10(5), 3 x 10(6), or 3 x 10(7) cells/graft. After 1 month, the domes were removed and one titanium implant was placed into each augmented site. One month after the second operation, the animals were killed and biopsy specimens were examined by histomorphometric and micro-CT analyses. Results indicated that at all concentrations, the ASC-loaded groups showed significantly more new bone formation and higher mean values of bone-implant contact and bone density inside threads than the ABB group. Furthermore, ASCs demonstrated a dose-response relationship, with the highest dose chosen inducing more robust bone regeneration. This study suggests that the delivery of ASCs on ABB might effectively increase vertical bone regeneration and implant osseointegration, versus ABB alone.
authors.affiliation: Department of Dental Sciences, University of Bologna, Via San Vitale 59, 40139 Bologna, Italy. checcopieri@yahoo.it
authors.forename: Francesco
authors.initials: F
authors.lastname: Pieri
authors.forename: Enrico
authors.initials: E
authors.lastname: Lucarelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Corinaldesi
authors.forename: Nicolò Nicoli
authors.initials: NN
authors.lastname: Aldini
authors.forename: Milena
authors.initials: M
authors.lastname: Fini
authors.forename: Annapaola
authors.initials: A
authors.lastname: Parrilli
authors.forename: Barbara
authors.initials: B
authors.lastname: Dozza
authors.forename: Davide
authors.initials: D
authors.lastname: Donati
authors.forename: Claudio
authors.initials: C
authors.lastname: Marchetti
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001861:Bone Regeneration; D002454:Cell Differentiation; D011817:Rabbits; D012886:Skull / Q000166:cytology*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014057:Tomography, X-Ray Computed