title: An HDAC inhibitor increases AcMNPV gene expression in mammalian cells.
abstract: The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is used as a safer viral vector in mammalian cells with potential applications in gene therapy. However, the mechanism for the insusceptibility of mammalian cells to proliferative infection by entomopathogenic viruses is not well understood. Here, we studied the significance of epigenetic modifications such as histone acetylation, histone methylation and HP1 accumulation for AcMNPV gene expression in mammalian BHK cells. Real-time PCR and chromatin immunoprecipitation with sodium butyrate revealed an important relationship between viral gene expression and histone acetylation, with implications for a mechanism of suppression of AcMNPV gene expression in BHK cells.
authors.affiliation: Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 001-8589, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Fujita
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ohtsuka
authors.forename: Ken
authors.initials: K
authors.lastname: Sahara
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Asano
authors.forename: Hisanori
authors.initials: H
authors.lastname: Bando
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D015967:Gene Expression Regulation, Viral; D056572:Histone Deacetylase Inhibitors / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D047468:Immunoprecipitation; D008647:Mesocricetus; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D016133:Polymerase Chain Reaction
chemical_list: D056572:Histone Deacetylase Inhibitors