title: Angiotensin I converting enzyme inhibitory peptide extracted from freshwater zooplankton.
abstract: In this study, hydrolysates obtained from the freshwater rotifer Brachionus calyciflonus were investigated for angiotensin I converting enzyme (ACE) inhibitory peptides. Freshwater rotifer protein was hydrolyzed using six separate enzymes in a batch reactor. The peptic hydrolysate had the highest ACE inhibitory activity compared to the other hydrolysates. The highest ACE inhibitory peptide was separated using Sephadex G-25 column chromatography and high-performance liquid chromatography on a C18 column. The 50% inhibitory concentration (IC(50)) value of purified ACE inhibitory peptide was 40.01 microg/mL. ACE inhibitory peptide was identified as being seven amino acid residues of Ala-Gln-Gly-Glu-Arg-His-Arg by N-terminal amino acid sequence analysis. The IC(50) value of purified ACE inhibitory peptide was 47.1 microM, and Lineweaver-Burk plots suggested that the peptide purified from rotifer protein acts as a competitive inhibitor against ACE. The results of this study suggest that peptides derived from freshwater rotifers may be beneficial as antihypertension compounds in functional foods or as pharmaceuticals.
authors.affiliation: Faculty of Marine Bioscience and Technology, Gangnueng-Wonju National University, Gangneung, Republic of Korea.
authors.forename: Jung Kwon
authors.initials: JK
authors.lastname: Lee
authors.forename: Min-Su
authors.initials: MS
authors.lastname: Lee
authors.forename: Heum Gi
authors.initials: HG
authors.lastname: Park
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
authors.forename: Hee-Guk
authors.initials: HG
authors.lastname: Byun
mesh_terms: D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001667:Binding, Competitive; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology*; D002845:Chromatography; D005618:Fresh Water; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*; D015048:Zooplankton / Q000737:chemistry*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D001685:Biological Factors; D010455:Peptides; D011506:Proteins