Formation of Schiff-base for photoreaction mechanism of red shift of GFP spectra.
We have proposed the formation of Schiff-base between R96 and chromophore (CRO) to elucidate the reaction mechanism for the irreversible red shift of green fluorescent protein (GFP) spectra under the absence of oxygen. The difference between absorption energies of reactant and product for our GFP models with CIS(D)/6-31G* level is 0.21eV, which is in reasonable agreement with the corresponding experimental value of 0.25eV. We have suggested the irreversible photoreaction mechanism, where the CRO excited from ground (S(0)) state to first excited singlet (S(1)) state immediately turns to the first excited triplet (T(1)) state, and the nucleophilic addition reaction occurs on the T(1) state.
Koseki
Jun
J
Quantum Chemistry Division, Graduate School of Science, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Japan.
Kita
Yukiumi
Y
Tachikawa
Masanori
M
D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D055668:Photochemical Processes; D011789:Quantum Theory; D012545:Schiff Bases / Q000138:chemical synthesis* / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D012545:Schiff Bases; D049452:Green Fluorescent Proteins