The cannabinoid 1-receptor silent antagonist O-2050 attenuates preference for high-fat diet and activated astrocytes in mice.
Endocannabinoids have been shown to activate reward-related feeding and to promote astrocytic differentiation. We investigated whether high-fat diet (HFD) intake produced a preference for HFD via an endocannabinoid-dependent mechanism. In the conditioned place preference test, the 2-week HFD-intake group showed preference for HFD and had increased expression of a marker for reactive astrocytes, glial fibrillary acid protein (GFAP), in the hypothalamus. The cannabinoid CB(1)-receptor antagonist O-2050 reduced the preference for HFD and expression of GFAP in the hypothalamus. These results suggested that HFD intake led to the development of a preference for HFD via astrocytic CB(1) receptors in the hypothalamus.
Higuchi
Sei
S
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
Irie
Keiichi
K
Mishima
Shohei
S
Araki
Maiko
M
Ohji
Makiko
M
Shirakawa
Atsunori
A
Akitake
Yoshiharu
Y
Matsuyama
Kiyoshi
K
Mishima
Kenji
K
Mishima
Kenichi
K
Iwasaki
Katsunori
K
Fujiwara
Michihiro
M
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage*; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology / Q000523:psychology; D005518:Food Preferences / Q000187:drug effects* / Q000502:physiology / Q000523:psychology; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011714:Pyrans / Q000494:pharmacology*; D043884:Receptor, Cannabinoid, CB1 / Q000037:antagonists & inhibitors* / Q000502:physiology
C508712:(6aR,10aR)-3-(1-methansulfonylamino-4-hexyn-6-yl)6a,7,10,10a-tetrahydro-6,6,9-trimethyl-6H-dibenzo(b,d)pyran; D004041:Dietary Fats; D011714:Pyrans; D043884:Receptor, Cannabinoid, CB1; D013759:Dronabinol