[Microwave radiation decreases the expressions of occludin and JAM-1 in rats].
OBJECTIVE
To explore the changes in the expressions of the tight junction related protein occludin and junctional adhesion molecule-1 (JAM-1) of the blood-testis barrier and their significance in rats after microwave radiation.


METHODS
Eighty male Wistar rats were exposed to microwave radiation with average power density of 0, 10, 30 and 100 mW/cm2 for five minutes, and dynamic changes in the expressions of testicular occludin and JAM-1 were observed by Western blot and image analysis at 6 h, 1 d, 3 d, 7 d and 14 d after the radiation.


RESULTS
There was a significant down-regulation in the expression of the occludin protein at 3 - 7 d, 6 h - 7 d and 6 h - 14 d (P < 0. 05), as well as in that of JAM-1 at 3 - 7 d, 1 - 7 d and 1-14 d (P < 0.05) after exposure to 10, 30 and 100 mW/cm2 microwave radiation.


CONCLUSIONS
The decreased protein expressions of occludin and JAM-1 may play an important role in the microwave radiation induced-damage to the blood-testis barrier.
Gao
Xiao-Fang
XF
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Wang
Shui-Ming
SM
Peng
Rui-Yun
RY
Zuo
Hong-Yan
HY
Wang
Li-Feng
LF
Gao
Ya-Bing
YB
Dong
Ji
J
Su
Zhen-Tao
ZT
D000818:Animals; D001814:Blood-Testis Barrier / Q000378:metabolism / Q000528:radiation effects; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D015536:Down-Regulation; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008872:Microwaves; D062793:Occludin; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000378:metabolism* / Q000528:radiation effects*
D015815:Cell Adhesion Molecules; C520495:F11r protein, rat; D008565:Membrane Proteins; D062793:Occludin; C568987:Ocln protein, rat