Inhibitory effects of devil's claw (secondary root of Harpagophytum procumbens) extract and harpagoside on cytokine production in mouse macrophages.
Successive oral administration (50 mg/kg) of a 50% ethanolic extract (HP-ext) of devil's claw, the secondary root of Harpagophytum procumbens, showed a significant anti-inflammatory effect in the rat adjuvant-induced chronic arthritis model. HP-ext dose-dependently suppressed the lipopolysaccharide (LPS)-induced production of inflammatory cytokines [interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha)] in mouse macrophage cells (RAW 264.7). Harpagoside, a major iridoid glycoside present in devil's claw, was found to be one of the active agents in HP-ext and inhibited the production of IL-1beta, IL-6, and TNF-alpha by RAW 264.7.
Inaba
Kazunori
K
Faculty of Pharmacy, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka, Japan.
Murata
Kazuya
K
Naruto
Shunsuke
S
Matsuda
Hideaki
H
D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D031684:Harpagophytum; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D011714:Pyrans / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley
D016207:Cytokines; D006027:Glycosides; D010936:Plant Extracts; D011714:Pyrans; C033249:harpagoside