title: Transgenic mouse model integrating siRNA targeting the foot and mouth disease virus.
abstract: We have constructed 2 small interfering RNAs (siRNAs) specifically targeting homogenous 3D and 2B1 regions of 7 serotypes of the foot and mouth disease virus (FMDV) and tested the ability of siRNAs to inhibit virus replication in baby hamster kidney (BHK-21) cells and suckling mice. In this study, we generated transgenic mouse models integrating short hairpin RNA (shRNA) targeting microinfected FMDV. When examined at the 7th passage in transgenic mice, the target gene was still found by PCR to be integrated in the genome. Compared to the control mice, the transgenic mice showed only slightly abnormal pathology when they were infected with the FMDV serotype Asia 1. The number of viruses in the tissues of the transgenic mouse was very low and in some tissues no virus could be detected by immunohistochemistry.
authors.affiliation: Key Laboratory for AgroBiotecnology of Shihezi University, Shihezi, Xinjiang 832003, PR China.
authors.forename: Wang
authors.initials: W
authors.lastname: Pengyan
authors.forename: Jiang
authors.initials: J
authors.lastname: Jianjun
authors.forename: Li
authors.initials: L
authors.lastname: Ning
authors.forename: Sheng
authors.initials: S
authors.lastname: Jinliang
authors.forename: Ren
authors.initials: R
authors.lastname: Yan
authors.forename: Chen
authors.initials: C
authors.lastname: Chuangfu
authors.forename: Guo
authors.initials: G
authors.lastname: Zhiru
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005536:Foot-and-Mouth Disease / Q000235:genetics* / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D015316:Genetic Therapy / Q000379:methods*; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D034741:RNA, Small Interfering / Q000235:genetics*; D019562:Viral Load; D014779:Virus Replication
chemical_list: D034741:RNA, Small Interfering