Fluorescent study of human blood plasma albumin alterations induced by ionizing radiation.
The use of hydrophobic fluorescent probe ABM (benzanthrone derivative) and albumin autofluorescence allowed show conformational alterations in Chernobyl clean-up workers blood plasma. Results obtained in 1996-1997 suggest that acidic expansion of plasma albumin takes place. Latest data (2006-2008) result in splitting of albumin alterations onto two stages - acidic expansion and N-F transition. The N-F transition is accompanied by the blue shift of fluorescence spectra and dehydration of tryptophanyl region of albumin molecule. In 2007 obtained.patterns of ABM spectra had never been previously seen in examined healthy individuals or patients with tuberculosis, multiple sclerosis, rheumatoid arthritis, etc. Patterns of ABM fluorescence spectra are associated with conformational changes of blood plasma albumin. The use of probe ABM and albumin auto-fluorescence allowed show conformational alterations in albumin of Chernobyl clean-up workers blood plasma. It is necessary to note that all investigated parameters significantly differ in observed groups of patients. These findings reinforce our understanding that the blood plasma albumin is a significant biological target of radiation. It may be concluded that fluorescence characteristics are representative of radiation induced albumin alterations and its carrier function.
Kirilova
Elena M
EM
Daugavpils University, Daugavpils, Latvia. elena.kirilova@inbox.lv
Kalnina
Inta
I
Zvagule
Tija
T
Gabruseva
Natalija
N
Kurjane
Natalja
N
Solomenikova
Irina I
II
D001551:Benz(a)Anthracenes; D047852:Chernobyl Nuclear Accident; D005456:Fluorescent Dyes; D006801:Humans; D016273:Occupational Exposure; D011487:Protein Conformation / Q000528:radiation effects; D011839:Radiation, Ionizing; D012709:Serum Albumin / Q000528:radiation effects*; D013050:Spectrometry, Fluorescence; D014364:Tryptophan; D014867:Water
D001551:Benz(a)Anthracenes; D005456:Fluorescent Dyes; D012709:Serum Albumin; D014867:Water; D014364:Tryptophan; C012768:benzanthrone