Melanin-concentrating hormone facilitates migration of preadipocytes.
Adipose tissue develops from differentiating preadipocytes that expand and migrate. 3T3-L1 preadipocytes respond to melanin-concentrating hormone (MCH) by increasing leptin production. Here, we investigate whether MCH elicits remodeling of the actin cytoskeleton and whether this translates into altered migratory capacity of these cells. Incubation with MCH resulted in a loss of actin stress fibers accompanied by a change in morphology from a stretched-out fibroblast to a rounded cell. PMC-3881-PI, a MCH receptor 1 antagonist blocked the effect, confirming this receptor is solely responsible for MCH-mediated actin rearrangements. Both a pharmacological activator and inhibitor of phospholipase C were used to demonstrate this molecule's importance to the signaling pathway. Finally, MCH was shown to facilitate preadipocyte migration into a scratch wound, revealing a previously unknown role for MCH in the regulation of cellular migration. We conclude that MCH could influence the expansion of adipose tissue through its ability to enhance preadipocyte migration.
Cook
Laurie B
LB
Department of Biology, The College at Brockport, State University of New York, 350 New Campus Drive, Brockport, NY 14420, USA. lcook@brockport.edu
Shum
Laura
L
Portwood
Scott
S
D041721:3T3-L1 Cells; D000199:Actins / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D006801:Humans; D007028:Hypothalamic Hormones / Q000494:pharmacology*; D008543:Melanins / Q000494:pharmacology*; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology; D010907:Pituitary Hormones / Q000494:pharmacology*; D011979:Receptors, Pituitary Hormone / Q000037:antagonists & inhibitors; D010738:Type C Phospholipases / Q000378:metabolism
C527780:Ac-Arg-(Cys-Met-Ava-Arg-Val-Tyr-Ava-Cys)-NH2; D000199:Actins; D007028:Hypothalamic Hormones; D008543:Melanins; D009842:Oligopeptides; D010907:Pituitary Hormones; D011979:Receptors, Pituitary Hormone; C092274:melanin-concentrating hormone receptor; C019463:melanin-concentrating hormone; D010738:Type C Phospholipases