Annexin-1 protects MCF7 breast cancer cells against heat-induced growth arrest and DNA damage.
Stress proteins protect cells against the effects of heat stress, such as cell death and DNA damage. We wished to determine if Annexin-1 (ANXA1) could mediate heat-induced growth arrest and DNA damage in MCF7 breast cancer cells. Heat induced a significant growth arrest at 4h-24h. Growth arrest and heat-induced DNA damage were significantly inhibited in MCF7 cells over-expressing ANXA1. These effects were associated with enhanced ERK activation and reduction in JNK phosphorylation. This study demonstrates that ANXA1, which we recently reported as a possible tumor suppressor gene, can protect cells from heat-induced growth arrest and DNA damage.
Nair
Sunitha
S
Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 28 Medical Drive, Singapore.
Hande
M Prakash
MP
Lim
Lina H K
LH
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D017305:Annexin A1 / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology*; D045744:Cell Line, Tumor; D020552:Comet Assay; D004249:DNA Damage / Q000235:genetics*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D018613:Microscopy, Confocal; D010957:Plasmids; D014162:Transfection; D014178:Translocation, Genetic
D017305:Annexin A1