title: Curcumin supplementation protects from genotoxic effects of arsenic and fluoride.
abstract: The present study was aimed to evaluate curcumin as a potential natural antioxidant to mitigate the genotoxic effects of arsenic (As) and fluoride (F) in human peripheral blood lymphocytes. The study was divided into nine groups consisting of negative control, positive control treated with ethyl methane sulphonate (EMS; 1.93 mM) and curcumin control with only curcumin (1.7 microM) in blood culture. As (1.4 microM) and F (34 microM) were added alone as well as in combination, to the cultures, with and without curcumin. Cultures were analysed for chromosomal aberrations (both structural and numerical) and primary DNA damage via comet assay as the genotoxic parameters after an exposure duration of 24h. Results revealed that curcumin efficiently ameliorates the toxic effect of As and F by reducing the frequency of structural aberrations (>60%), hypoploidy (>50%) and primary DNA damage. In conclusion, curcumin mitigates the genotoxic effects of the two well known water contaminants (As and F) effectively and efficiently at the given concentration in vitro.
authors.affiliation: Zoology Department, University School of Sciences, Gujarat University, Navrangpura, Ahmedabad, India. hemlata.pant@yahoo.com
authors.forename: Hemlata
authors.initials: H
authors.lastname: Tiwari
authors.forename: Mandava V
authors.initials: MV
authors.lastname: Rao
mesh_terms: D000328:Adult; D000782:Aneuploidy; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D000077237:Arsenic Trioxide; D001152:Arsenicals; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000139:chemically induced; D019457:Chromosome Breakage / Q000187:drug effects; D020552:Comet Assay; D003474:Curcumin / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D010087:Oxides / Q000633:toxicity*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012969:Sodium Fluoride / Q000633:toxicity*; D055815:Young Adult
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D001152:Arsenicals; D010087:Oxides; D010835:Phytohemagglutinins; D012969:Sodium Fluoride; D003474:Curcumin; D000077237:Arsenic Trioxide