title: 3D collagen scaffolds coated with multiwalled carbon nanotubes: initial cell attachment to internal surface.
abstract: The cell adhesion in a multiwalled carbon nanotube-coated collagen sponge (MWCNT-coated sponge) was investigated. Immediately after seeding, the cells adhered to the inner surface of the MWCNT-coated sponge and a significantly larger number of cells were observed there than for a pure collagen sponge used as control. On the MWCNT-coated sponge, the cells appeared favorable adhesion and spread in the early stages in the center part of the sponge which cells rarely attached without MWCNT-coating. It was suggested that the physical structure of MWCNTs was effective for initial adhesion of cells from the result of serum-free culture. MWCNT-coating makes the material a suitable three-dimensional scaffold for cell culturing, as opposed to other scaffold systems where such an effect is not seen.
authors.affiliation: Department of Oral Functional Prosthodontics, Division of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Kita-ku, Sapporo, Japan. erieri@den.hokudai.ac.jp
authors.forename: Eri
authors.initials: E
authors.lastname: Hirata
authors.forename: Motohiro
authors.initials: M
authors.lastname: Uo
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Nodasaka
authors.forename: Hiroko
authors.initials: H
authors.lastname: Takita
authors.forename: Natsumi
authors.initials: N
authors.lastname: Ushijima
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Akasaka
authors.forename: Fumio
authors.initials: F
authors.lastname: Watari
authors.forename: Atsuro
authors.initials: A
authors.lastname: Yokoyama
mesh_terms: D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible; D003094:Collagen; D006801:Humans; D037742:Nanotubes, Carbon; D054457:Tissue Scaffolds
chemical_list: D020099:Coated Materials, Biocompatible; D037742:Nanotubes, Carbon; D003094:Collagen