title: Investigation of tumor hyperpolarized [1-13C]-pyruvate dynamics using time-resolved multiband RF excitation echo-planar MRSI.
abstract: Hyperpolarized [1-(13)C]-pyruvate is an exciting new agent for the in vivo study of cellular metabolism and a potential cancer biomarker. The nature of the hyperpolarized signal poses unique challenges because of its short duration and the loss of magnetization with every excitation. In this study, we applied a novel and efficient time-resolved MR spectroscopic imaging (MRSI) method to investigate in a prostate cancer model the localized temporal dynamics of the uptake of [1-(13)C]-pyruvate and its conversion to metabolic products, specifically [1-(13)C]-lactate. This hyperpolarized (13)C method used multiband excitation pulses for efficient use of the magnetization. This study demonstrated that regions of tumor were differentially characterized from normal tissue by the lactate dynamics, where tumors showed later lactate detection and longer lactate duration that was statistically significant (P < 0.001). Compared to late-pathologic-stage tumors, early- to intermediate-stage tumors demonstrated significantly (P < 0.01) lower lactate total signal-to-noise ratio (SNR), with similar temporal dynamic parameters. Hyperpolarized pyruvate dynamics provided uptake, perfusion, and vascularization information on tumors and normal tissue. Large, heterogeneous tumors demonstrated spatially variable uptake of pyruvate and metabolic conversion that was consistent with cellularity and necrosis identified by histology. The results of this study demonstrated the potential of this new hyperpolarized MR dynamic method for improved cancer detection and characterization.
authors.affiliation: Department of Radiology and Biomedical Imaging, University of California-San Francisco, San Francisco, California 94158, USA. peder.larson@radiology.ucsf.edu
authors.forename: Peder E Z
authors.initials: PE
authors.lastname: Larson
authors.forename: Robert
authors.initials: R
authors.lastname: Bok
authors.forename: Adam B
authors.initials: AB
authors.lastname: Kerr
authors.forename: Michael
authors.initials: M
authors.lastname: Lustig
authors.forename: Simon
authors.initials: S
authors.lastname: Hu
authors.forename: Albert P
authors.initials: AP
authors.lastname: Chen
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Nelson
authors.forename: John M
authors.initials: JM
authors.lastname: Pauly
authors.forename: John
authors.initials: J
authors.lastname: Kurhanewicz
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Vigneron
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D017352:Echo-Planar Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D019289:Pyruvic Acid / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002247:Carbon Isotopes; D019289:Pyruvic Acid