title: Interaction of an essential Escherichia coli GTPase, Der, with the 50S ribosome via the KH-like domain.
abstract: Der, an essential Escherichia coli tandem GTPase, has been implicated in 50S subunit biogenesis. The rrmJ gene encodes a methyltransferase that modifies the U2552 residue of 23S rRNA, and its deletion causes a severe growth defect. Peculiarly, overexpression of Der suppresses growth impairment. In this study, using an rrmJ-deletion strain, we demonstrated that two GTPase domains of Der regulate its association with 50S subunit via the KH-like domain. We also identified a region of Der that is critical for its specific interaction with 50S subunit.
authors.affiliation: Center for Advanced Biotechnology and Medicine, Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 679 Hoes Lane, Piscataway, NJ 08854, USA.
authors.forename: Jihwan
authors.initials: J
authors.lastname: Hwang
authors.forename: Masayori
authors.initials: M
authors.lastname: Inouye
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008780:Methyltransferases / Q000235:genetics / Q000502:physiology; D016296:Mutagenesis; D010641:Phenotype; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D054681:Ribosome Subunits, Large, Bacterial / Q000235:genetics / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D029968:Escherichia coli Proteins; D008780:Methyltransferases; C102247:rlmE protein, E coli; C515037:Der protein, E coli; D019204:GTP-Binding Proteins