title: Effects of ebselen versus nimodipine on cerebral vasospasm subsequent to experimental subarachnoid hemorrhage in rats.
abstract: We investigated the effect of ebselen relative to nimodipine in an animal model of subarachnoid hemorrhage. Thirty Wistar albino rats were divided into 5 groups: G1, no intervention; G2, sham surgery without subarachnoid hemorrhage (SAH); G3, SAH only; G4, SAH plus nimodipine treatment; G5, SAH plus ebselen treatment. For G2 animals, physiological saline (0.9% NaCl) was injected into the cisterna magna. For G3, G4 and G5 animals, SAH was induced by injecting autologous non-heparinized blood into the cisterna magna. One hour after injection, G4 animals received nimodipine at 6-hour intervals and G5 animals received ebselen twice a day for 48 hours. After treatment, brain tissue and blood samples were taken for biochemical and histopathological examination. Mean malonyldialdehyde concentration was significantly higher in G3 than in G1 (p<0.0001), G2 (p=0.01), G4 (p=0.002) and G5 (p=0.014), and significantly higher in G5 than in G1 (p=0.013). Mean superoxide dismutase activity was significantly lower in G4 than in both G1 (p=0.025) and G2 (p=0.02). Mean wall thickness was significantly greater in G3 than in G1 (p<0.0001), G2 (p=0.01), G4 (p<0.0001) and G5 (p<0.0001). Mean wall thickness was also significantly greater in both G1 and G2 than in G4 (p<0.0014 and p<0.0001) and G5 (p<0.0001 and p<0.0001). Mean luminal diameter of the basilar artery was significantly smaller in G3 than in G2 (p=0.02), G4 (p<0.018) and G5 (p<0.001). Our results confirm that ebselen may have neuroprotective effects by acting to prevent vasospasm.
authors.affiliation: Department of Neurosurgery, School of Medicine, Zonguldak Karaelmas University, Esenkoy, Kozlu, Zonguldak 67600, Turkey. drsanser@yahoo.com <drsanser@yahoo.com>
authors.forename: Sanser
authors.initials: S
authors.lastname: Gul
authors.forename: Burak
authors.initials: B
authors.lastname: Bahadir
authors.forename: Volkan
authors.initials: V
authors.lastname: Hanci
authors.forename: Serefden
authors.initials: S
authors.lastname: Acikgoz
authors.forename: Sibel
authors.initials: S
authors.lastname: Bektas
authors.forename: Ebru
authors.initials: E
authors.lastname: Ugurbas
authors.forename: Handan
authors.initials: H
authors.lastname: Ankarali
authors.forename: Murat
authors.initials: M
authors.lastname: Kalayci
authors.forename: Bektaş
authors.initials: B
authors.lastname: Acikgoz
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D001393:Azoles / Q000008:administration & dosage*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004334:Drug Administration Schedule; D005260:Female; D007276:Injections, Intraventricular; D054833:Isoindoles; D008315:Malondialdehyde / Q000378:metabolism; D009553:Nimodipine / Q000008:administration & dosage*; D016566:Organoselenium Compounds / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013345:Subarachnoid Hemorrhage / Q000150:complications* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014665:Vasodilator Agents / Q000008:administration & dosage; D020301:Vasospasm, Intracranial / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001393:Azoles; D054833:Isoindoles; D016566:Organoselenium Compounds; D014665:Vasodilator Agents; C042986:ebselen; D008315:Malondialdehyde; D009553:Nimodipine; D013482:Superoxide Dismutase