Detoxified endotoxin vaccine (J5dLPS/OMP) protects mice against lethal respiratory challenge with Francisella tularensis SchuS4.
Francisella tularensis is a category A select agent. J5dLPS/OMP is a novel vaccine construct consisting of detoxified, O-polysaccharide side chain-deficient, lipopolysaccharide non-covalently complexed with the outer membrane protein of N. meningitidis group B. Immunization elicits high-titer polyclonal antibodies specific for the highly-conserved epitopes expressed within the glycolipid core that constitutes gram-negative bacteria (e.g., F. tularensis). Mice immunized intranasally with J5dLPS/OMP exhibited protective immunity to intratracheal challenge with the live vaccine strain, as well as the highly-virulent SchuS4 strain, of F. tularensis. The efficacy of J5dLPS/OMP vaccine suggests its potential utility in immunizing the general population against several different gram-negative select agents concurrently.
Gregory
Stephen H
SH
Department of Medicine, Rhode Island Hospital and The Warren Alpert Medical School of Brown University, 55 Claverick Street, Providence, RI 02903, USA. sgregory@lifespan.org
Chen
Wilbur H
WH
Mott
Stephanie
S
Palardy
John E
JE
Parejo
Nicholas A
NA
Heninger
Sara
S
Anderson
Christine A
CA
Artenstein
Andrew W
AW
Opal
Steven M
SM
Cross
Alan S
AS
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D015169:Colony Count, Microbial; D004731:Endotoxins / Q000276:immunology*; D005260:Female; D005604:Francisella tularensis / Q000276:immunology*; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D008070:Lipopolysaccharides / Q000276:immunology*; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009345:Neisseria meningitidis / Q000276:immunology; D044967:Serum / Q000276:immunology / Q000382:microbiology; D016019:Survival Analysis; D014406:Tularemia / Q000517:prevention & control*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D004731:Endotoxins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D008070:Lipopolysaccharides