title: The glycoproteins C and G are equivalent target antigens for the determination of herpes simplex virus type 1-specific antibodies.
abstract: Seroreactivity to the glycoproteins C and G of herpes simplex virus type 1 (HSV-1) was compared in 310 serum samples using a Western blot assay containing a whole antigen extract of HSV-1 and an ELISA employing gC1 isolated from HSV-1. The prevalence of reactivity to gC1 was 75.8% by Western blot and 73.9% by ELISA, while antibody responses to gG1 were detected in 72.9% of sera by Western blot. An absolute correlation of 96.1% between the reactivity to gC1 and gG1 was demonstrated using the Western blot. The gC1-based ELISA correlated with Western blot detection of anti-gC1 and anti-gG1 antibodies in 95.2 and 97.7% of samples, respectively. 3.2% of all sera were reactive with gC1 in Western blot and/or ELISA, but were negative for anti-gG1. For analysis of cross-reactivity, antibodies against HSV-2, Epstein-Barr virus, varicella-zoster virus and cytomegalovirus were determined. The prevalence of antibodies against each individual virus was identical in the groups of sera reactive with gC1 or gG1. These findings indicate that gC1 and gG1 are equivalent antigenic targets for the type-specific serodiagnosis of HSV-1 infections.
authors.affiliation: Institute of Experimental Immunology, affiliated to EUROIMMUN AG, Seekamp 31, D-23560 Luebeck, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Scheper
authors.forename: Sandra
authors.initials: S
authors.lastname: Saschenbrecker
authors.forename: Katja
authors.initials: K
authors.lastname: Steinhagen
authors.forename: Andreas
authors.initials: A
authors.lastname: Sauerbrei
authors.forename: Waltraud
authors.initials: W
authors.lastname: Suer
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Meyer
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schlumberger
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Wandinger
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D015153:Blotting, Western / Q000379:methods; D003587:Cytomegalovirus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006561:Herpes Simplex / Q000175:diagnosis* / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000276:immunology; D014645:Herpesvirus 3, Human / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; C050591:glycoprotein gG-1, herpes simplex virus type 1