title: Use of the kinase inhibitor analog 1NM-PP1 reveals a role for Toxoplasma gondii CDPK1 in the invasion step.
abstract: Toxoplasma gondii CDPK1 (TgCDPK1) was found to be the target of the toxoplasmocidal compound 1NM-PP1. When TgCDPK1 was mutated at position 128 from glycine to methionine, resistance was gained. Inhibition of gliding motility without inhibition of micronemal secretion by 1NM-PP1 suggests a function for TgCDPK1 in gliding motility.
authors.affiliation: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Tatsuki
authors.initials: T
authors.lastname: Sugi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kato
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Shumpei
authors.initials: S
authors.lastname: Watanabe
authors.forename: Hitomi
authors.initials: H
authors.lastname: Kurokawa
authors.forename: Haiyan
authors.initials: H
authors.lastname: Gong
authors.forename: Kishor
authors.initials: K
authors.lastname: Pandey
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Takemae
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Akashi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D011247:Pregnancy; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D011720:Pyrazoles / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D016415:Sequence Alignment; D014122:Toxoplasma / Q000187:drug effects* / Q000502:physiology*
chemical_list: C479693:1-tert-butyl-3-naphthalen-1-ylmethyl-1H-pyrazolo(3,4-d)pyrimidin-4-ylemine; D047428:Protein Kinase Inhibitors; D015800:Protozoan Proteins; D011720:Pyrazoles; D011743:Pyrimidines; D011494:Protein Kinases