Preparative isolation of flavonoid compounds from Oroxylum indicum by high-speed counter-current chromatography by using ionic liquids as the modifier of two-phase solvent system.
A preparative high-speed counter-current chromatography method for isolation and purification of flavonoid compounds from Oroxylum indicum was successfully established by using ionic liquids as the modifier of the two-phase solvent system. Two flavonoid compounds including baicalein-7-O-diglucoside and baicalein-7-O-glucoside were purified from the crude extract of O. indicum by using ethyl acetate-water-[C(4)mim][PF(6)] (5:5:0.2, v/v) as two-phase solvent system. 36.4 mg of baicalein-7-O-diglucoside and 60.5 mg of baicalein-7-O-glucoside were obtained from 120 mg of the crude extract. Their purities were 98.7 and 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of the isolated compounds were identified by (1)H-NMR and (13)C-NMR.
Liu
Renmin
R
College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, PR China. liurenmin@lcu.edu.cn
Xu
Lili
L
Li
Aifeng
A
Sun
Ailing
A
D029402:Bignoniaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification*; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D052578:Ionic Liquids / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D013997:Time Factors
D004187:Disaccharides; D047309:Flavones; D005960:Glucosides; D052578:Ionic Liquids; D010936:Plant Extracts; D012997:Solvents; C550990:baicalein-7-O-diglucoside; C550991:baicalein-7-O-glucoside