title: Hybrid superparamagnetic iron oxide nanoparticle-branched polyethylenimine magnetoplexes for gene transfection of vascular endothelial cells.
abstract: The work demonstrated the development of thermally cross-linked superparamagnetic nanomaterial which possessed polyethylene glycol moiety and covalently linked branched polyethylenimine (BPEI), and exhibited highly efficient magnetofection even under serum conditioned media. The study showed its high anti-biofouling, cell viability and serum stability and thus revealed a potential magnetic nanoparticle-mediated targeted gene delivery system. This superparamagnetic particle mediated rapid and efficient transfection in primary vascular endothelial cells (HUVEC) successfully inhibits expression of PAI-1 which is responsible for various vascular dysfunctions such as vascular inflammation and atherosclerosis and thereby provides a potential strategy to transfect highly sensitive HUVEC. The sequential steps for the enhanced magnetofection had been studied by monitoring cellular uptake with the aid of confocal microscopy.
authors.affiliation: Department of Chemistry, BK21 Program, Polymer Research Institute, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Ran
authors.initials: R
authors.lastname: Namgung
authors.forename: Kaushik
authors.initials: K
authors.lastname: Singha
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Yu
authors.forename: Sangyong
authors.initials: S
authors.lastname: Jon
authors.forename: Yong Sook
authors.initials: YS
authors.lastname: Kim
authors.forename: Youngkeun
authors.initials: Y
authors.lastname: Ahn
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Park
authors.forename: Won Jong
authors.initials: WJ
authors.lastname: Kim
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D004247:DNA / Q000378:metabolism; D003911:Dextrans; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology*; D052203:Ferrosoferric Oxide / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D008280:Magnetics / Q000379:methods*; D058185:Magnetite Nanoparticles; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010957:Plasmids / Q000235:genetics; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D011094:Polyethyleneimine / Q000378:metabolism*; D013499:Surface Properties; D013997:Time Factors; D014162:Transfection / Q000379:methods*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; D017395:Plasminogen Activator Inhibitor 1; D014409:Tumor Necrosis Factor-alpha; D011094:Polyethyleneimine; D004247:DNA; C065507:ferumoxides; D016650:Fluorescein-5-isothiocyanate; D052203:Ferrosoferric Oxide