Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L.).
Cucumber mosaic virus (CMV) is one of the most destructive viruses in the Solanaceae family. Simple inheritance of CMV resistance in peppers has not previously been documented; all previous studies have reported that resistance to this virus is mediated by several partially dominant and recessive genes. In this study, we showed that the Capsicum annuum cultivar 'Bukang' contains a single dominant resistance gene against CMV(Korean) and CMV(FNY) strains. We named this resistance gene Cmr1 (Cucumber mosaic resistance 1). Analysis of the cellular localization of CMV using a CMV green fluorescent protein construct showed that in 'Bukang,' systemic movement of the virus from the epidermal cell layer to mesophyll cells is inhibited. Genetic mapping and FISH analysis revealed that the Cmr1 gene is located at the centromeric region of LG2, a position syntenic to the ToMV resistance locus (Tm-1) in tomatoes. Three SNP markers were developed by comparative genetic mapping: one intron-based marker using a pepper homolog of Tm-1, and two SNP markers using tomato and pepper BAC sequences mapped near Cmr1. We expect that the SNP markers developed in this study will be useful for developing CMV-resistant cultivars and for fine mapping the Cmr1 gene.
Kang
Won-Hee
WH
Department of Plant Science, Research Institute for Agriculture and Life Sciences, Plant Genomics and Breeding Institute, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Korea.
Hoang
Ngoc Huy
NH
Yang
Hee-Bum
HB
Kwon
Jin-Kyung
JK
Jo
Sung-Hwan
SH
Seo
Jang-Kyun
JK
Kim
Kook-Hyung
KH
Choi
Doil
D
Kang
Byoung-Cheorl
BC
D002212:Capsicum / Q000235:genetics* / Q000821:virology*; D002874:Chromosome Mapping / Q000379:methods; D017799:Cucumovirus / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005799:Genes, Dominant; D017343:Genes, Plant; D017404:In Situ Hybridization, Fluorescence; D018551:Solanum lycopersicum / Q000235:genetics / Q000821:virology; D018613:Microscopy, Confocal / Q000379:methods; D008957:Models, Genetic; D010944:Plants / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D018744:DNA, Plant