title: Influence of antagonist sensory and sympathetic nerves on smooth muscle cell differentiation in hypercholesterolemic rat.
abstract: The effect of sympathectomy and sensory denervation on vascular smooth muscle cell (SMC) differentiation was investigated in hypercholesterolemic rats. Newborn rats received injections of guanethidine, capsaicin or both for denervations. Shams received injections of vehicles. The four groups were fed 1% cholesterol diet for 3 months. Intact normocholesterolemic rats were also exploited. Serum total cholesterol and systolic blood pressure (SBP) were measured. Lipid presence in the arterial wall was shown by Red-Oil-O staining. Catecholamine- and CGRP-containing fibres, vimentin and the adult SMC markers alpha-SMC-actin, desmin and h-caldesmon were analysed in abdominal aorta by western blot and confocal microscope. The sympathetic (catecholamine) fibres and SBP increased after sensory denervation while the sensory (CGRP) fibres increased and SBP decreased after sympathectomy. SBP was not changed after double denervation. Total cholesterol increased in sham and rose further after sympathectomy. Vimentin and the three adult SMC markers were not influenced by hypercholesterolemia. However, in the sympathectomized aorta, vimentin increased, desmin did not change, whereas alpha-SMC-actin and h-caldesmon decreased. In the sensory-denervated aorta, vimentin decreased, desmin increased, alpha-SMC-actin did not change and h-caldesmon decreased but less than in sympathectomized aorta. In the doubly denervated aorta, vimentin did not change and the three adult SMC markers decreased, although less than in sympathectomized aorta for alpha-SMC-actin and h-caldesmon. Thickened intima was identified by Red-Oil-O staining in the sympathectomized and (less remarkably) doubly denervated aortas containing SMCs not fully dedifferentiated. Our findings suggest that sympathectomy induces intimal thickening and favours SMC dedifferentiation, whereas sensory denervation favours SMC differentiation.
authors.affiliation: Unité de Physiologie Intégré, Laboratoire de Pathologies Vasculaires. Faculté des Sciences de Bizerte, Jarzouna, Tunisia.
authors.forename: Rafik
authors.initials: R
authors.lastname: Hachani
authors.forename: Houcine
authors.initials: H
authors.lastname: Dab
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
authors.forename: Eric
authors.initials: E
authors.lastname: Vicaut
authors.forename: Jacques
authors.initials: J
authors.lastname: Callebert
authors.forename: Richard
authors.initials: R
authors.lastname: Sercombe
authors.forename: Kamel
authors.initials: K
authors.lastname: Kacem
mesh_terms: D000199:Actins / Q000096:biosynthesis; D000320:Adrenergic Fibers / Q000378:metabolism*; D000818:Animals; D001012:Aorta, Abdominal / Q000166:cytology* / Q000294:innervation*; D001794:Blood Pressure; D015153:Blotting, Western; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis; D002148:Calmodulin-Binding Proteins / Q000096:biosynthesis; D002454:Cell Differentiation; D003714:Denervation; D006937:Hypercholesterolemia / Q000503:physiopathology*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar; D011984:Sensory Receptor Cells / Q000378:metabolism*; D017539:Tunica Intima / Q000166:cytology / Q000378:metabolism; D017540:Tunica Media / Q000166:cytology / Q000378:metabolism
chemical_list: D000199:Actins; D002148:Calmodulin-Binding Proteins; D015740:Calcitonin Gene-Related Peptide