Enhanced expression of lymphomagenesis-related genes in peripheral blood B cells of chronic hepatitis C patients.
Epidemiological data indicate a close relationship between chronic hepatitis C virus (HCV) infection and B-cell non-Hodgkin's lymphoma (B-NHL), suggesting that chronic HCV infection is, at least in part, associated with B-lymphomagenesis. However, experimental data concerning these conditions remains elusive. In this study, we confirmed that peripheral blood B cells of chronic hepatitis C (CHC) patients were infected with HCV. Expression levels of activation-induced cytidine deaminase (AID) which are thought to be associated with occurrence of B-NHL were analyzed in these CHC B cells. It was demonstrated that AID mRNA/protein levels in CHC B cells were dramatically increased compared with those of healthy subjects. Furthermore, expression levels of several previously reported prognostic B-NHL marker genes in the B cell subset of CHC patients were increased. These results suggest a possible relationship between chronic HCV infection and B-lymphomagenesis.
Ito
Masahiko
M
Department of Research on Blood and Biological Products, National Institute of Infectious Diseases, Musashi-Murayama-shi, Tokyo 208-0011, Japan.
Murakami
Kyoko
K
Suzuki
Tetsuro
T
Mochida
Keiko
K
Suzuki
Miho
M
Ikebuchi
Kenji
K
Yamaguchi
Kazunari
K
Mizuochi
Toshiaki
T
D001402:B-Lymphocytes / Q000821:virology*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D003564:Cytidine Deaminase / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D019698:Hepatitis C, Chronic / Q000097:blood / Q000150:complications / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000821:virology*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D012367:RNA, Viral; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase