Sulfatides are associated with neointimal thickening after vascular injury.
BACKGROUND
Sulfatides are known to be a native ligand of P-selectin. Platelet-leukocyte interaction via the cross-talk between P-selectin and Mac-1 (CD11b/CD18) plays an important role in the mechanism of neointimal thickening after vascular injury such as that seen in post-stent restenosis. However, the roles of sulfatides on restenosis have not been elucidated.


METHODS
Serum sulfatide levels, P-selectin expression on the surface of platelets, and activated Mac-1 on the surface of neutrophils were serially measured using both coronary sinus and peripheral blood samples in 21 patients who underwent coronary stent implantation.


RESULTS
The trans-cardiac gradient (coronary sinus minus peripheral blood) of the sulfatide levels significantly increased at 15 min (-1.47+/-2.87 to 0.59+/-1.44 nmol/ml, p<0.001), compared to baseline levels. The maximum response of the trans-cardiac gradient of P-selectin expression on the surface of platelets at 15 min after stent implantation (R=0.55, p<0.01), and that of activated Mac-1 on the surface of neutrophils at 48 h (R=0.59, p<0.01), were both positively correlated with that of serum sulfatide levels at 15 min. The angiographic late lumen loss was correlated with the trans-cardiac gradient of sulfatide levels at 15 min (R=0.48, p<0.05), platelet P-selectin expression at 15 min (R=0.42, p<0.05) and activated neutrophil Mac-1 expression at 48 h (R=0.46, p<0.05), but not with values at other sampling points.


CONCLUSIONS
Sulfatides may play a physiological role on inflammation in vascular injury and the development of neointimal thickening.
Inoue
T
T
Department of Cardiovascular Medicine, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Tochigi, 321-0293 Japan. inouet@dokkyomed.ac.jp
Taguchi
I
I
Abe
S
S
Li
G
G
Hu
R
R
Nakajima
T
T
Hara
A
A
Aoyama
T
T
Kannagi
R
R
Kyogashima
M
M
Node
K
K
D000368:Aged; D015906:Angioplasty, Balloon, Coronary; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood; D016177:Macrophage-1 Antigen / Q000097:blood; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood* / Q000378:metabolism; D015607:Stents; D013433:Sulfoglycosphingolipids / Q000097:blood* / Q000378:metabolism; D017539:Tunica Intima / Q000473:pathology
D016177:Macrophage-1 Antigen; D019007:P-Selectin; D013433:Sulfoglycosphingolipids