title: In situ preparation of magnetic nonviral gene vectors and magnetofection in vitro.
abstract: Magnetic nonviral gene vectors were in situ prepared in the presence of ferrous salts and hyperbranched poly(ethylenimine)s (HPEI) with different molecular weights. HPEI, one of the most promising nonviral vectors, was not only utilized as the nanoreactor and stabilizer to prepare magnetic nanoparticles, but also skillfully used as a base supplier to avoid introducing alkali hydroxide or ammonia. Magnetic nonviral gene vectors with various magnetite contents and saturation magnetizations were obtained by changing the weight ratio of HPEI to FeSO(4).7H(2)O and the molecular weight of HPEI. MTT assays suggested that the resulting magnetite/HPEI gene vectors had lower cytotoxicity compared with pure HPEI. The magnetite/HPEI nonviral gene vectors were used for magnetofection. It was found that the luciferase expression level mediated by magnetite/HPEI in COS-7 cells under a magnetic gradient field was approximately 13-fold greater than that of standard HPEI transfection.
authors.affiliation: School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Shi
authors.forename: Linzhu
authors.initials: L
authors.lastname: Zhou
authors.forename: Ruibin
authors.initials: R
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Pang
authors.forename: Wangchuan
authors.initials: W
authors.lastname: Xiao
authors.forename: Huiqin
authors.initials: H
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Su
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Wang
authors.forename: Bangshang
authors.initials: B
authors.lastname: Zhu
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Zhu
authors.forename: Deyue
authors.initials: D
authors.lastname: Yan
authors.forename: Hongchen
authors.initials: H
authors.lastname: Gu
mesh_terms: D000818:Animals; D019556:COS Cells; D002470:Cell Survival; D002522:Chlorocebus aethiops; D004247:DNA / Q000008:administration & dosage*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D011094:Polyethyleneimine / Q000737:chemistry*; D014162:Transfection
chemical_list: D011094:Polyethyleneimine; D004247:DNA; D052203:Ferrosoferric Oxide