Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF-3196. Induction, isolation and properties.
The laccase induction in submerged culture of basidiomycete Cerrena unicolor VKM F-3196 was investigated. Cu(2+) at concentration 0.1 mM was an optimum inducer of C. unicolor laccase. Two isoforms of laccase, namely LacC1 and LacC2, were isolated and characterized. The isoforms were shown to have different physical-chemical and catalytic properties. On the basis of the MALDI TOF MS analysis of tryptic cleavage products of both the proteins and N-terminal amino-acid sequences analysis two isoforms of laccase (LacC1 and LacC2) were classified as products of two different genes.
Lisova
Zoya Alexandrovna
ZA
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Russian Federation, Pushchino, Moscow region, Prospekt Nauki. zoyapozhid@rambler.ru
Lisov
Alexander Viktorovich
AV
Leontievsky
Alexey Arkadievich
AA
D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D002241:Carbohydrates / Q000737:chemistry; D003470:Culture Media; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D005800:Genes, Fungal; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification; D042845:Laccase / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature
D002241:Carbohydrates; D003470:Culture Media; D005656:Fungal Proteins; D007527:Isoenzymes; D042845:Laccase