Flavonol quantification and stability of phenolics in fermented extracts from fresh Betula pendula leaves.
An HPLC method, which allows reliable quantitation of flavonols and other phenolics in birch leaf extracts, was developed and validated. The method was applied to study the bioconversion of flavonols in fermented aqueous extracts. Almost 100% of the flavonols were converted during the 12 months observation period. The generated phenolics as well as consecutive conversion products were identified by HPLC-DAD, LC-MS and GC-MS techniques.
Millet
Agnès
A
Institute for Pharmaceutical Sciences, Department of Pharmaceutical Biology and Biotechnology, Albert-Ludwig-University Freiburg, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
Stintzing
Florian
F
Merfort
Irmgard
I
D029662:Betula / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D004355:Drug Stability; D005285:Fermentation; D044948:Flavonols / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D008956:Models, Chemical; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis
D044948:Flavonols; D010636:Phenols; D010936:Plant Extracts; C021304:hyperoside; D011794:Quercetin