title: CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors.
abstract: BACKGROUND
  Tumor initiating cells (TICs) provide a new paradigm for developing original therapeutic strategies.
  
  
  METHODS
  We screened for TICs in 47 human adult brain malignant tumors. Cells forming floating spheres in culture, and endowed with all of the features expected from tumor cells with stem-like properties were obtained from glioblastomas, medulloblastoma but not oligodendrogliomas.
  
  
  RESULTS
  A long-term self-renewal capacity was particularly observed for cells of malignant glio-neuronal tumors (MGNTs). Cell sorting, karyotyping and proteomic analysis demonstrated cell stability throughout prolonged passages. Xenografts of fewer than 500 cells in Nude mouse brains induced a progressively growing tumor. CD133, CD15/LeX/Ssea-1, CD34 expressions, or exclusion of Hoechst dye occurred in subsets of cells forming spheres, but was not predictive of their capacity to form secondary spheres or tumors, or to resist high doses of temozolomide.
  
  
  CONCLUSIONS
  Our results further highlight the specificity of a subset of high-grade gliomas, MGNT. TICs derived from these tumors represent a new tool to screen for innovative therapies.
authors.affiliation: Glial Plasticity lab, Inserm UMR 894, University Paris Descartes, Paris, France.
authors.forename: Cristina
authors.initials: C
authors.lastname: Patru
authors.forename: Luciana
authors.initials: L
authors.lastname: Romao
authors.forename: Pascale
authors.initials: P
authors.lastname: Varlet
authors.forename: Laure
authors.initials: L
authors.lastname: Coulombel
authors.forename: Eric
authors.initials: E
authors.lastname: Raponi
authors.forename: Josette
authors.initials: J
authors.lastname: Cadusseau
authors.forename: François
authors.initials: F
authors.lastname: Renault-Mihara
authors.forename: Cécile
authors.initials: C
authors.lastname: Thirant
authors.forename: Nadine
authors.initials: N
authors.lastname: Leonard
authors.forename: Alain
authors.initials: A
authors.lastname: Berhneim
authors.forename: Maria
authors.initials: M
authors.lastname: Mihalescu-Maingot
authors.forename: Jacques
authors.initials: J
authors.lastname: Haiech
authors.forename: Ivan
authors.initials: I
authors.lastname: Bièche
authors.forename: Vivaldo
authors.initials: V
authors.lastname: Moura-Neto
authors.forename: Catherine
authors.initials: C
authors.lastname: Daumas-Duport
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Junier
authors.forename: Hervé
authors.initials: H
authors.lastname: Chneiweiss
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis*; D018952:Antigens, CD34 / Q000096:biosynthesis*; D001932:Brain Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D016256:Lewis X Antigen / Q000096:biosynthesis*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000166:cytology*; D009474:Neurons / Q000473:pathology*; D010455:Peptides; D040901:Proteomics / Q000379:methods
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; D016256:Lewis X Antigen; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse