title: High dose of ascorbic acid induces cell death in mesothelioma cells.
abstract: Malignant mesothelioma is an asbestos-related fatal disease with no effective cure. Recently, high dose of ascorbate in cancer treatment has been reexamined. We studied whether high dose of ascorbic acid induced cell death of four human mesothelioma cell lines. High dose of ascorbic acid induced cell death of all mesothelioma cell lines in a dose-dependent manner. We further clarified the cell killing mechanism that ascorbic acid induced reactive oxygen species and impaired mitochondrial membrane potential. In vivo experiment, intravenous administration of ascorbic acid significantly decreased the growth rate of mesothelioma tumor inoculated in mice. These data suggest that ascorbic acid may have benefits for patients with mesothelioma.
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors.forename: Yukitoshi
authors.initials: Y
authors.lastname: Takemura
authors.forename: Motohiko
authors.initials: M
authors.lastname: Satoh
authors.forename: Kiyotoshi
authors.initials: K
authors.lastname: Satoh
authors.forename: Hironobu
authors.initials: H
authors.lastname: Hamada
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Sekido
authors.forename: Shunichiro
authors.initials: S
authors.lastname: Kubota
mesh_terms: D000818:Animals; D017209:Apoptosis; D001205:Ascorbic Acid / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008654:Mesothelioma / Q000188:drug therapy*; D051379:Mice; D016513:Mice, SCID; D017382:Reactive Oxygen Species / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D017382:Reactive Oxygen Species; D001205:Ascorbic Acid