Low-potential detection of endogenous and physiological uric acid at uricase-thionine-single-walled carbon nanotube modified electrodes.
This work develops and validates an electrochemical approach for uric acid (UA) determinations in both endogenous (cell lysate) and physiological (serum) samples. This approach is based on the electrocatalytic reduction of enzymatically generated H(2)O(2) at the biosensor of uricase-thionine-single-walled carbon nanotube/glassy carbon (UOx-Th-SWNTs/GC) with the use of Th-SWNTs nanostructure as a mediator and an enzyme immobilization matrix. The biosensor, which was fabricated by immobilizing UOx on the surface of Th-SWNTs, exhibited a rapid response (ca. 2 s), a low detection limit (0.5 +/- 0.05 microM), a wide linear range (2 microM to 2 mM), high sensitivity (approximately 90 microA mM(-1) cm(-2)), as well as good stability and repeatability. In addition, the common interfering species, such as ascorbic acid, 3,4-dihydroxyphenylacetic acid, 4-acetamidophenol, etc., did not cause any interference due to the use of a low operating potential (-400 mV vs saturated calomel electrode). Therefore, this work has demonstrated a simple and effective sensing platform for selective detection of UA in the physiological levels. In particular, the developed approach could be very important and useful to determine the relative role of endogenous and physiological UA in various conditions such as hypertension and cardiovascular disease.
Chen
Dongxiao
D
Jiangsu Key Laboratory of Biofunctional Materials, Laboratory of Electrochemistry, College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing 210046, People's Republic of China.
Wang
Qian
Q
Jin
Juan
J
Wu
Ping
P
Wang
Hui
H
Yu
Shuqin
S
Zhang
Hui
H
Cai
Chenxin
C
D001407:Bacillus / Q000201:enzymology; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D002460:Cell Line; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057230:Limit of Detection; D037742:Nanotubes, Carbon / Q000737:chemistry*; D013997:Time Factors; D014503:Urate Oxidase / Q000737:chemistry / Q000378:metabolism; D014527:Uric Acid / Q000032:analysis*
D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D014527:Uric Acid; D014503:Urate Oxidase