Activity of siRNAs with 2-thio-2'-O-methyluridine modification in mammalian cells.
In a search to identify chemical modifications to improve the properties of siRNA, we have investigated the effect of the 2 '-O-methyl-2-thiouridine modification on the biological activity of siRNA. Our results indicate that judicious placement of 2 '-O-methyl-2-thiouridine residues could lead to modified siRNA with activity in mammalian cells.
Prakash
Thazha P
TP
Department of Medicinal Chemistry, Isis Pharmaceuticals Inc., Carlsbad, California 92008, USA. tprakashn@isisph.com
Naik
Nishant
N
Sioufi
Namir
N
Bhat
Balkrishen
B
Swayze
Eric E
EE
D002478:Cells, Cultured; D015195:Drug Design; D006367:HeLa Cells; D006801:Humans; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism
C409967:2'-O-methyl-2-thiouridine; D009841:Oligonucleotides; D034741:RNA, Small Interfering; D013891:Thiouridine