title: Construction, screening and identification of a phage display antibody library against the Eimeria acervulina merozoite.
abstract: A single-chain antibody library against Eimeria acervulina merozoites was constructed by phage display approach. Antibody-displaying phage was selected in four panning rounds against cryopreserved E. acervulina merozoites. Five clones were randomly selected from the fourth panning round, and their nucleotide sequences were aligned and compared to mouse germ-line sequences. Soluble antibody was produced in a non-suppressor Escherichia coli strain, purified by protein A affinity chromatography, and characterized by Western-blotting. Immunofluorescence assay showed localization of the produced recombinant antibody fragment on the surface E. acervulina merozoites. These resultant antibody fragments showed high specificity and binding capacity for soluble antigens and intact fixed merozoites which seems promising as diagnostic, therapeutic and/or vaccine tools against coccidiosis.
authors.affiliation: College of Chinese Traditional Veterinary Science, Agricultural University of Hebei, Dingzhou 073000, China.
authors.forename: Yuelan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Said
authors.initials: S
authors.lastname: Amer
authors.forename: Jianyong
authors.initials: J
authors.lastname: Wang
authors.forename: Chengmin
authors.initials: C
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.forename: Guiying
authors.initials: G
authors.lastname: Kang
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Bao
authors.forename: Hongxuan
authors.initials: H
authors.lastname: He
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D004539:Eimeria / Q000276:immunology*; D052940:Merozoites / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D019151:Peptide Library; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology*
chemical_list: D019151:Peptide Library; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies