title: Automated microscopy for high-content RNAi screening.
abstract: Fluorescence microscopy is one of the most powerful tools to investigate complex cellular processes such as cell division, cell motility, or intracellular trafficking. The availability of RNA interference (RNAi) technology and automated microscopy has opened the possibility to perform cellular imaging in functional genomics and other large-scale applications. Although imaging often dramatically increases the content of a screening assay, it poses new challenges to achieve accurate quantitative annotation and therefore needs to be carefully adjusted to the specific needs of individual screening applications. In this review, we discuss principles of assay design, large-scale RNAi, microscope automation, and computational data analysis. We highlight strategies for imaging-based RNAi screening adapted to different library and assay designs.
authors.affiliation: Advanced Light Microscopy Core Facility, European Molecular Biology Laboratory Heidelberg, D-69117 Heidelberg, Germany. christian.conrad@embl.de
authors.forename: Christian
authors.initials: C
authors.lastname: Conrad
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Gerlich
mesh_terms: D000818:Animals; D001331:Automation / Q000379:methods*; D023281:Genomics; D057166:High-Throughput Screening Assays / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D034622:RNA Interference; D049490:Systems Biology