title: Cloning and characterization of microRNAs from porcine skeletal muscle and adipose tissue.
abstract: MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that regulate the stability and translation of cognate mRNAs. Although an increasing number of porcine miRNAs has recently been identified, the full repertoire of miRNAs in pig remains to be elucidated. To identify porcine miRNAs potentially involved in myogenesis and adipogenesis, we constructed small RNA cDNA libraries from skeletal muscle and adipose tissue and identified 89 distinct miRNAs that are conserved in pig, of which 15 were new. Expression analysis of all newly identified and selected known porcine miRNAs revealed that some miRNAs were enriched in a tissue-specific manner, whereas others were expressed ubiquitously in the porcine tissues examined. Our results expand the number of known porcine miRNAs and provide useful information for further investigating the biological functions of miRNAs associated with growth and development of skeletal muscle or adipose tissue in pig.
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Anam-Dong, Seongbuk-Gu, Seoul, 136-713, South Korea.
authors.forename: Ik Sang
authors.initials: IS
authors.lastname: Cho
authors.forename: Jung
authors.initials: J
authors.lastname: Kim
authors.forename: Hye Youn
authors.initials: HY
authors.lastname: Seo
authors.forename: Do Hwan
authors.initials: DH
authors.lastname: Lim
authors.forename: Jae Sang
authors.initials: JS
authors.lastname: Hong
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Park
authors.forename: Dae Cheul
authors.initials: DC
authors.lastname: Park
authors.forename: Ki-Chang
authors.initials: KC
authors.lastname: Hong
authors.forename: Kwang Youn
authors.initials: KY
authors.lastname: Whang
authors.forename: Young Sik
authors.initials: YS
authors.lastname: Lee
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016678:Genome / Q000235:genetics; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D012322:RNA Precursors / Q000737:chemistry / Q000235:genetics; D017423:Sequence Analysis, RNA; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012322:RNA Precursors