Transcriptional profile of genes involved in the biosynthesis of phytate and ferritin in Coffea.
The present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic background. The roles of the investigated genes are discussed concerning the quality of coffee beverage.
Nobile
Paula M
PM
Centro de Genetica, Instituto Agronomico de Campinas, Caixa Postal 28, Campinas, SP, Brazil. paulanobile@yahoo.com
Quecini
Vera
V
Bazzo
Barbara
B
Quiterio
Gabriela
G
Mazzafera
Paulo
P
Colombo
Carlos A
CA
D040503:Coffea / Q000235:genetics* / Q000378:metabolism; D005293:Ferritins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010833:Phytic Acid / Q000096:biosynthesis*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism
D010940:Plant Proteins; D010833:Phytic Acid; D005293:Ferritins