Effects of cytokines on long control region transcriptional activity in high-risk cutaneous human papillomavirus types 5 and 8.
Cytokines play an important role in the control of mucosal HPV transcription. However, there is little data available on cutaneous HPVs, which are associated with non-melanoma skin cancers. Here, we describe a cell-based assay exploiting HaCaT keratinocytes stably transfected with a reporter construct containing the long control region (LCR) regulatory sequence of gene transcription in HPV-5 and HPV-8. This novel assay has allowed the first systematic analysis of the effects of cytokines on HPV-5 and HPV-8 LCR activity and provides a valuable tool for the search for cutaneous HPV-gene expression inhibitors.
Donalisio
Manuela
M
Department of Clinical and Biological Sciences, S. Luigi Gonzaga Hospital, University of Turin, Regione Gonzole, 10, 10043 Orbassano, Turin, Italy.
Poli
Alice
A
Civra
Andrea
A
Landolfo
Santo
S
Lembo
David
D
D019677:Artificial Gene Fusion; D016207:Cytokines / Q000276:immunology*; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter; D006801:Humans; D015603:Keratinocytes / Q000821:virology; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D027383:Papillomaviridae / Q000276:immunology* / Q000502:physiology*; D014158:Transcription, Genetic
D016207:Cytokines; D008156:Luciferases