title: The dependence of enhanced lysosomal activity on the cellular aging of bovine aortic endothelial cells.
abstract: Lysosomes are a cell organelle type processing antimicrobial activity. Here, we investigate the lysosomal activity in a primary cell line, bovine aortic endothelial cells (BAECs), during cellular aging, based on the antimicrobial activity of lysosomes isolated from BAECs at cell passages 4, 6, 8, and 10. Cytochemical analysis of lysosomes with LysoTracker reagent revealed the number of lysosome-like organelles surrounding the nucleus initially increased drastically in the BAECs and continued increasing gradually until passage 10. The lysosomes isolated from each successive passage of BAECs exhibited increased antimicrobial activity against Escherichia coli, and, in addition, an age-dependent increase in lysosome intensity coincided with increased lysosomal antimicrobial activity.
authors.affiliation: Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561-756, South Korea.
authors.forename: Jihee
authors.initials: J
authors.lastname: Yoon
authors.forename: Ki-Ju
authors.initials: KJ
authors.lastname: Kim
authors.forename: Young-Woo
authors.initials: YW
authors.lastname: Choi
authors.forename: Hwa Sung
authors.initials: HS
authors.lastname: Shin
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
mesh_terms: D000588:Amines; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000201:enzymology; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence; D015169:Colony Count, Microbial; D042783:Endothelial Cells / Q000201:enzymology*; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D005456:Fluorescent Dyes; D008247:Lysosomes / Q000201:enzymology*; D018613:Microscopy, Confocal; D013997:Time Factors
chemical_list: D000588:Amines; D005456:Fluorescent Dyes; C493330:Red DND-99