Preparation and recognition properties of bovine hemoglobin magnetic molecularly imprinted polymers.
A simple method for the preparation of core-shell micro/nanostructured magnetic molecularly imprinted polymers (MIPs) for protein recognition is described. Magnetic MIPs were synthesized by copolymering gamma-aminopropyltrimethoxysilane and tetraethyl orthosilicate at the surface of Fe(3)O(4) nanospheres, which were directly covalently bound with template molecule, bovine hemoglobin (BHb), through imine bond. Transmission electron microscopy and scanning electron microscopy images showed that the Fe(3)O(4) nanospheres with diameter about 50-150 nm were coated with the MIPs layer with average thickness about 10 nm, which enabled the magnetic MIPs to have a sensitive and fast magnetic response. The proximity between the thickness of MIPs layer and the spatial size of BHb indicated that the imprinted sites almost situated at the surface of magnetic MIPs, leading a rapid adsorption saturation within 1 h. And the adsorption amounts of magnetic MIPs toward BHb were estimated to be 10.52 mg/g at pH 6.5, which was 4.6 times higher than that of magnetic nonmolecularly imprinted polymers. Meanwhile, the result of selective test showed that the magnetic MIPs had an excellent recognition capacity to BHb compared to the other nontemplate proteins. Except for the spatial size complementary between BHb and the binding sites in magnetic MIPs, the electrostatic interaction also was proven to be an important factor for recognizing the imprinting molecule.
Kan
Xianwen
X
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093, People's Republic of China.
Zhao
Qun
Q
Shao
Dalin
D
Geng
Zhirong
Z
Wang
Zhilin
Z
Zhu
Jun-Jie
JJ
D000327:Adsorption; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D005290:Ferric Compounds / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D008280:Magnetics; D054802:Molecular Imprinting; D049329:Nanostructures / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D055672:Static Electricity
D005290:Ferric Compounds; D006454:Hemoglobins; D011108:Polymers; C000499:ferric oxide