title: Anti-ulcerogenic effect and HPLC analysis of the caffeoylquinic acid-rich extract from Ligularia stenocephala.
abstract: The leaves of three Ligularia species belonging to the family Compositae, Ligularia stenocephala, L. fischeri, and L. fischeri var. spiciformis, were qualitatively and quantitatively analyzed on the caffeoylquinic acids by HPLC and subjected to peroxynitrite-scavenging assay. The IC(50) of the MeOH extract of L. stenocephala was 1.62+/-0.03 mug/ml and the major caffeoylquinic acids of L. stenocephala were 5-O-caffeoylquinic acid, 3,5-di-O-caffeoyl-muco-quinic acid, and 3,5-di-O-caffeoylquinic acid. The compositions of caffeoylquinic acids were different for the three plants. Since percentage of total caffeoylquinic acids of the extract was highest (42.20% of the MeOH extract and 94.52% of the BuOH extract) in L. stenocephala and potent in peroxynitrite-scavenging assay, the extracts of L. stenocephala were chosen to perform in vivo anti-ulcerogenic activity. Treatment of mice with the MeOH- and BuOH extracts decreased the diameter of gastric lesions caused by HCl/ethanol- and indomethacin/bethanechol and decreased the volume of gastric juice, suggesting that caffeoylquinic acids have anti-ulcerogenic activity. These results suggest that the leaves of Ligularia species may help prevent or treat gastric ulcers.
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon, Korea.
authors.forename: Byung-Il
authors.initials: BI
authors.lastname: Lee
authors.forename: Agung
authors.initials: A
authors.lastname: Nugroho
authors.forename: Moch Saiful
authors.initials: MS
authors.lastname: Bachri
authors.forename: Jongwon
authors.initials: J
authors.lastname: Choi
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
authors.forename: Won-Bae
authors.initials: WB
authors.lastname: Kim
authors.forename: Kyung-Tae
authors.initials: KT
authors.lastname: Lee
authors.forename: Jong-Dai
authors.initials: JD
authors.lastname: Lee
authors.forename: Hee-Juhn
authors.initials: HJ
authors.lastname: Park
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D019659:Asteraceae / Q000737:chemistry*; D018723:Bethanechol; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D005750:Gastric Juice / Q000378:metabolism; D006851:Hydrochloric Acid; D007213:Indomethacin; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D030421:Peroxynitrous Acid / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D013045:Species Specificity; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000897:Anti-Ulcer Agents; D010936:Plant Extracts; C472707:caffeoylquinic acid; D018723:Bethanechol; D011801:Quinic Acid; D030421:Peroxynitrous Acid; D000431:Ethanol; D006851:Hydrochloric Acid; D007213:Indomethacin