title: Single-molecule chemical reactions on DNA origami.
abstract: DNA nanotechnology and particularly DNA origami, in which long, single-stranded DNA molecules are folded into predetermined shapes, can be used to form complex self-assembled nanostructures. Although DNA itself has limited chemical, optical or electronic functionality, DNA nanostructures can serve as templates for building materials with new functional properties. Relatively large nanocomponents such as nanoparticles and biomolecules can also be integrated into DNA nanostructures and imaged. Here, we show that chemical reactions with single molecules can be performed and imaged at a local position on a DNA origami scaffold by atomic force microscopy. The high yields and chemoselectivities of successive cleavage and bond-forming reactions observed in these experiments demonstrate the feasibility of post-assembly chemical modification of DNA nanostructures and their potential use as locally addressable solid supports.
authors.affiliation: Centre for DNA Nanotechnology (CDNA) at the Interdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000 Aarhus C, Denmark.
authors.forename: Niels V
authors.initials: NV
authors.lastname: Voigt
authors.forename: Thomas
authors.initials: T
authors.lastname: Tørring
authors.forename: Alexandru
authors.initials: A
authors.lastname: Rotaru
authors.forename: Mikkel F
authors.initials: MF
authors.lastname: Jacobsen
authors.forename: Jens B
authors.initials: JB
authors.lastname: Ravnsbaek
authors.forename: Ramesh
authors.initials: R
authors.lastname: Subramani
authors.forename: Wael
authors.initials: W
authors.lastname: Mamdouh
authors.forename: Jørgen
authors.initials: J
authors.lastname: Kjems
authors.forename: Andriy
authors.initials: A
authors.lastname: Mokhir
authors.forename: Flemming
authors.initials: F
authors.lastname: Besenbacher
authors.forename: Kurt Vesterager
authors.initials: KV
authors.lastname: Gothelf
mesh_terms: D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D055767:Immobilized Proteins / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D010316:Particle Size; D019809:Streptavidin / Q000737:chemistry
chemical_list: D055767:Immobilized Proteins; D001710:Biotin; D004247:DNA; D019809:Streptavidin