Determination of adrenolytic drugs by SPME-LC-MS.
Five adrenolytic drugs have been analyzed by liquid chromatography-mass spectrometry (LC-MS). Samples were prepared by solid-phase microextraction (SPME) using polypyrrole fibers coated on stainless steel support as an adsorbent for the drugs. Adsorption efficiencies were 95% and were close for all the drugs investigated. Relative standard deviations (RSD), calculated for samples prepared in standard solutions, were in the range 2.5-13%, however RSD values for the drugs in human plasma were 2.5-4.5%. Using LC-MS the limit of detection (LOD) and the limit of quantification (LOQ) were in the ranges 0.11-0.18 and 0.39-0.54 ng mL(-1), respectively, for the five drugs.
Buszewski
Boguslaw
B
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100, Torun, Poland. bbusz@chem.uni.torun.pl.
Olszowy
Pawel
P
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100, Torun, Poland.
Ligor
Tomasz
T
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100, Torun, Poland.
Szultka
Malgorzata
M
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100, Torun, Poland.
Nowaczyk
Jacek
J
Department of Physical Chemistry and Physicochemistry of Polymers, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100, Torun, Poland.
Jaworski
Maciej
M
Department of Surgery, Collegium Medicum, Nicolaus Copernicus University, St. Joseph Street 53-59, 85-067, Bydgoszcz, Poland.
Jackowski
Marek
M
Department of Surgery, Collegium Medicum, Nicolaus Copernicus University, St. Joseph Street 53-59, 85-067, Bydgoszcz, Poland.
D018674:Adrenergic Antagonists / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D052617:Solid Phase Microextraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D018674:Adrenergic Antagonists