Ultra-fast HPLC-ICP-MS analysis of oxaliplatin in patient urine.
A novel method for rapid HPLC-ICP-MS analysis of oxaliplatin in human urine was developed implementing a stationary HPLC phase with a particle size of 1.8 microm. The method allowed a cycle time of <1 min at a HPLC flow rate of 0.9 mL min(-1). Procedural limits of detection of 0.05 microg L(-1) oxaliplatin (150 fg on column) were obtained. Analysis of oxaliplatin in patient urine showed that accurate quantification of the intact drug demanded for storage at -80 degrees C and rapid measurement after thawing.
Koellensperger
Gunda
G
Department of Chemistry, Division of Analytical Chemistry, University of Natural Resources and Applied Life Sciences, BOKU, Vienna, Muthgasse 18, 1190, Vienna, Austria.
Hann
Stephan
S
Department of Chemistry, Division of Analytical Chemistry, University of Natural Resources and Applied Life Sciences, BOKU, Vienna, Muthgasse 18, 1190, Vienna, Austria. stephan.hann@boku.ac.at.
D000970:Antineoplastic Agents / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009944:Organoplatinum Compounds / Q000652:urine*; D000077150:Oxaliplatin
D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin