title: Swine intestine antimicrobial peptides inhibit infectious bronchitis virus infectivity in chick embryos.
abstract: Infectious bronchitis virus (IBV), the causative agent of infectious bronchitis, results in respiratory disease, nephritis, and poor egg production and quality in chicken. Antimicrobial peptides possess potent antimicrobial activities and are regarded as promising therapeutic alternatives in the fight against microorganisms. To assess the in vitro antiviral activity of swine intestine antimicrobial peptides (SIAMP) against IBV, 45 chick embryos were randomly assigned into 3 groups, 15 for each group. Embryos in group 1 were inoculated with IBV. Group 2 was inoculated with SIAMP-IBV intermixture. Group 3 was used as a control and inoculated with sterile PBS. Allantoic fluid was collected for hemagglutination titer assay. In addition, weight gain, mortality, and pathological changes for each group were recorded. The results showed that no distinct pathological changes were found in chick embryos after they were inoculated with SIAMP-IBV intermixture and the mortality was reduced remarkably compared with the IBV-infected group. Weight gain of embryos in the SIAMP-IBV intermixture group was significantly higher than the IBV-infected embryos (P < 0.01), which was also higher than the control group (P < 0.05). Furthermore, the hemagglutination titer in the SIAMP-IBV group was significantly lower than that in the IBV-infected group (P < 0.01). These results indicated that SIAMP can inhibit virus replication and reduce tissue injury caused by IBV.
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China. sunquancau@yahoo.com
authors.forename: Q
authors.initials: Q
authors.lastname: Sun
authors.forename: K
authors.initials: K
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: She
authors.forename: W
authors.initials: W
authors.lastname: Ma
authors.forename: F
authors.initials: F
authors.lastname: Peng
authors.forename: H
authors.initials: H
authors.lastname: Jin
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D000998:Antiviral Agents / Q000627:therapeutic use; D001921:Brain / Q000473:pathology; D002642:Chick Embryo / Q000821:virology*; D018352:Coronavirus Infections / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D001351:Infectious bronchitis virus; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000473:pathology; D011201:Poultry Diseases / Q000188:drug therapy* / Q000473:pathology / Q000821:virology; D011531:Proventriculus / Q000473:pathology; D013552:Swine
chemical_list: D023181:Antimicrobial Cationic Peptides; D000998:Antiviral Agents