title: Comparison of traditional phenotypic identification methods with partial 5' 16S rRNA gene sequencing for species-level identification of nonfermenting Gram-negative bacilli.
abstract: Correct identification of nonfermenting Gram-negative bacilli (NFB) is crucial for patient management. We compared phenotypic identifications of 96 clinical NFB isolates with identifications obtained by 5' 16S rRNA gene sequencing. Sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify NFB.
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108, USA. cloudjl@aruplab.com
authors.forename: Joann L
authors.initials: JL
authors.lastname: Cloud
authors.forename: Dag
authors.initials: D
authors.lastname: Harmsen
authors.forename: Peter C
authors.initials: PC
authors.lastname: Iwen
authors.forename: James J
authors.initials: JJ
authors.lastname: Dunn
authors.forename: Gerri
authors.initials: G
authors.lastname: Hall
authors.forename: Paul Rocco
authors.initials: PR
authors.lastname: Lasala
authors.forename: Karen
authors.initials: K
authors.lastname: Hoggan
authors.forename: Deborah
authors.initials: D
authors.lastname: Wilson
authors.forename: Gail L
authors.initials: GL
authors.lastname: Woods
authors.forename: Alexander
authors.initials: A
authors.lastname: Mellmann
mesh_terms: D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S