Evaluating binuclear copper(II) complexes for glycoside hydrolysis.
Three binuclear copper(II) complexes were characterized as solids by X-ray diffraction and in solution by UV/vis spectrophotometric titration, and subsequently evaluated for their glycosidase-like activity. The structure analysis revealed comparable intermetallic Cu...Cu distances (approximately 3.5 A) for the complexes 2 and 3. Despite this similarity, the composition of the complexes differs significantly in aqueous solution as revealed by spectrophotometric titrations. The hydrolysis of selected nitrophenylglycopyranosides is up to 11,000-fold accelerated over background in the presence of the copper(II) complexes in 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS) buffer at pH 10.5 and 30 degrees C.
Striegler
Susanne
S
Department of Chemistry and Biochemistry, 179 Chemistry Building, Auburn University, Auburn, Alabama 36849, USA. susanne.striegler@auburn.edu
Dunaway
Natasha A
NA
Gichinga
Moses G
MG
Barnett
James D
JD
Nelson
Anna-Gay D
AG
D002384:Catalysis; D003300:Copper / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008958:Models, Molecular; D015394:Molecular Structure; D013055:Spectrophotometry, Infrared; D014961:X-Ray Diffraction
D006027:Glycosides; D003300:Copper