Evaluation of the tissue damage of porcine ovaries after bipolar drilling under transvaginal hydrolaparoscopy--an in vitro experiment.
OBJECTIVE
To evaluate the tissue damage of in vitro porcine ovarian drilling in saline solution mimicking transvaginal hydrolaparoscopy (THL).


METHODS
In vitro ovarian drilling with different current and activation time was applied on fresh porcine ovaries, 15 ovaries in each group and 2 punctures in each ovary. Drilling using 5-Fr bipolar needle was performed in saline solution mimicking THL, while saline solution rinsing the ovaries after monopolar electrocoagulation.


RESULTS
The monopolar drilling caused more tissue damage than the bipolar needle in saline (p < 0.01). The ratio of the damage of monopolar electrocoagulation (40 w, 3s) over that of bipolar diathermy in saline solution (70 w, 15 s) was 7.4 [(16.74 +/- 1.30) mm(3)/(2.27 +/- 0.49) mm(3)]. In the bipolar groups, the 70 w power set (15s and 20 s) caused significantly more tissue damage than the 50 w ones (p < 0.05), and the amount of damage was not stimulation time dependent (p > 0.05).


CONCLUSIONS
The tissue damage caused by monopolar electrocoagulation with 40 w for 3 s was about seven times of that caused by 5-Fr bipolar one in saline with 70 w for 15 s . In THL drilling using a 5-Fr bipolar electrode, the current is more crucial than stimulation time.
Ma
Cai-Hong
CH
Center for Reproductive Medicine, Peking University Third Hospital, Beijing, China.
Yang
Shuo
S
Qiao
Jie
J
Yang
Yan
Y
Liu
Ping
P
D000704:Analysis of Variance; D000818:Animals; D004564:Electrocoagulation / Q000379:methods*; D005260:Female; D010535:Laparoscopy / Q000379:methods*; D009339:Needles; D010053:Ovary / Q000601:surgery*; D013552:Swine