Heterologous expression of Anabaena PCC 7120 all3940 (a Dps family gene) protects Escherichia coli from nutrient limitation and abiotic stresses.
This study presents first hand data on the cloning and heterologous expression of Anabaena PCC 7120 all3940 (a dps family gene) in combating nutrients limitation and multiple abiotic stresses. The Escherichia coli transformed with pGEX-5X-2-all3940 construct when subjected to iron, carbon, nitrogen, phosphorus limitation and carbofuron, copper, UV-B, heat, salt and cadmium stress registered significant increase in growth over the cells transformed with empty vector under iron (0%), carbon (0.05%), nitrogen (3.7 mM) and phosphorus (2mM) limitation and carbofuron (0.025 mg ml(-1)), CuCl(2) (1 mM), UV-B (10 min), heat (47 degrees C), NaCl (6% w/v) and CdCl(2) (4mM) stress. Enhanced expression of all3940 gene measured by semi-quantitative RT-PCR at different time points under above mentioned treatments clearly demonstrates its role in tolerance against aforesaid abiotic stresses. This study opens the gate for developing transgenic cyanobacteria capable of growing successfully under above mentioned stresses.
Narayan
Om Prakash
OP
Molecular Biology Section, Laboratory of Algal Biology, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi-22 1005, India.
Kumari
Nidhi
N
Rai
Lal Chand
LC
D017033:Anabaena / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D019256:Cadmium Chloride / Q000633:toxicity; D002244:Carbon / Q000172:deficiency; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D015870:Gene Expression; D006358:Hot Temperature; D000090463:Iron Deficiencies; D009584:Nitrogen / Q000172:deficiency; D010758:Phosphorus / Q000172:deficiency; D013312:Stress, Physiological / Q000235:genetics*; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C083911:DPS protein, Bacteria; D010758:Phosphorus; D002244:Carbon; D019256:Cadmium Chloride; D009584:Nitrogen