Analyses of functional IL10 and TNF-α genotypes in Behçet's syndrome.
We aim to ascertain the possible involvement of functional IL10 and TNF-α promoter polymorphisms on the susceptibility to Behçet's syndrome (BS), to examine whether IL10 and TNF-α genotypes might work synergistically influencing susceptibility to BS. IL10 -1082G/A, -819C/T and -592C/A and TNF -308G/A polymorphisms were analyzed in 102 Turkish patients with BS and 102 healthy subjects by using amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). We have found no significant associations between IL10 -1082G/A, -819C/T, -592C/A, TNF-α -308G/A polymorphisms and BS. Also, no significant correlation was found between IL10 GCC, ACC, ATA haplotypes, GCC(+)/GCC(+), GCC(+)/GCC(-), GCC(-)/GCC(-) genotypes. There was no significant association between combined TNF-α/IL10 genotypes and BS. Our study indicates that functional TNF-α, IL10 genotypes or combined TNF-α, IL10 genotypes do not play a role in BS susceptibility in Turkish BS patients.
Ateş
Omer
O
Department of Molecular Biology and Genetics, Science Faculty, Istanbul University, Istanbul, Turkey. omerates27@yahoo.com
Dalyan
Levent
L
Hatemi
Gulen
G
Hamuryudan
Vedat
V
Topal-Sarıkaya
Aysegul
A
D000328:Adult; D001528:Behcet Syndrome / Q000235:genetics*; D016022:Case-Control Studies; D003710:Demography; D005260:Female; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
C508609:IL10 protein, human; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10