Simple and conveniently accessible bi-fluorescence-labeled substrates for amylases.
Synthesis of bi-fluorescence-labeled maltooligosaccharides for amylase assay was accomplished. Preliminary biological evaluation of both bi-fluorescence-labeled maltohexasaccharide and maltose using alpha-amylase was carried out, and the hexaosyl derivative showed unique variation on the basis of fluorescence resonance energy transfer (FRET).
Oka
Hiroyuki
H
Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama, Japan.
Koyama
Tetsuo
T
Hatano
Ken
K
Terunuma
Daiyo
D
Matsuoka
Koji
K
D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity; D000516:alpha-Amylases / Q000378:metabolism*
D005456:Fluorescent Dyes; D000516:alpha-Amylases