title: Expression of tissue inhibitors of metalloproteinase 1 (TIMP-1) in gastric cancer tissue.
abstract: Degradation of extracellular matrix (ECM) is an essential step of invasion and metastasis of gastric cancer. The proteolysis of basement membranes depends on the balance between activities of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs). The aim of the study was to assess the expression of TIMP-1 in gastric cancer (GC) and interstitial inflammatory infiltrate cells within GC tissue in relation to clinico-pathological features of tumor and to estimate the prognostic significance of TIMP-1 expression for patients' survival. The presence of TIMP-1 in 54 cases of gastric cancer samples was investigated by immunohistochemistry. The expression of TIMP-1 in cancer and interstitial inflammatory infiltrate cells was evaluated in semi-quantitative scale. The immunoreactivity of TIMP-1 in cancer and inflammatory cells was positive in 100% of cases and varied from weak to intense reaction. The intensity of TIMP-1 expression increased with more advanced tumor stages and in patients who died of cancer during 2-year observation. TIMP-1 expression in interstitial inflammatory infiltrate cells was the independent prognostic factor for patients' survival. The results suggest the role of TIMP-1 in gastric tumorigenesis, although this issue requires further investigtions.
authors.affiliation: Department of Biochemical Diagnostics, Medical University, Białystok, Poland. mroczko@umwb.edu.pl
authors.forename: Barbara
authors.initials: B
authors.lastname: Mroczko
authors.forename: Marta
authors.initials: M
authors.lastname: Lukaszewicz-Zajac
authors.forename: Magdalena
authors.initials: M
authors.lastname: Groblewska
authors.forename: Jolanta
authors.initials: J
authors.lastname: Czyzewska
authors.forename: Mariusz
authors.initials: M
authors.lastname: Gryko
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Guzińska-Ustymowicz
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kemona
authors.forename: Bogusław
authors.initials: B
authors.lastname: Kedra
authors.forename: Maciej
authors.initials: M
authors.lastname: Szmitkowski
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D012044:Regression Analysis; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D015996:Survival Rate; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1