title: Interplay between the NADP-linked thioredoxin and glutathione systems in Arabidopsis auxin signaling.
abstract: Intracellular redox status is a critical parameter determining plant development in response to biotic and abiotic stress. Thioredoxin (TRX) and glutathione are key regulators of redox homeostasis, and the TRX and glutathione pathways are essential for postembryonic meristematic activities. Here, we show by associating TRX reductases (ntra ntrb) and glutathione biosynthesis (cad2) mutations that these two thiol reduction pathways interfere with developmental processes through modulation of auxin signaling. The triple ntra ntrb cad2 mutant develops normally at the rosette stage, undergoes the floral transition, but produces almost naked stems, reminiscent of the phenotype of several mutants affected in auxin transport or biosynthesis. In addition, the ntra ntrb cad2 mutant shows a loss of apical dominance, vasculature defects, and reduced secondary root production, several phenotypes tightly regulated by auxin. We further show that auxin transport capacities and auxin levels are perturbed in the mutant, suggesting that the NTR-glutathione pathways alter both auxin transport and metabolism. Analysis of ntr and glutathione biosynthesis mutants suggests that glutathione homeostasis plays a major role in auxin transport as both NTR and glutathione pathways are involved in auxin homeostasis.
authors.affiliation: Laboratoire Génome et Développement des Plantes, Université de Perpignan, Unité Mixte de Recherche, Centre National de la Recherche Scientifique, Institut de Recherche pour le Développement-Université de Perpignan Via Domitia 5096, 66860 Perpignan, France.
authors.forename: Talaat
authors.initials: T
authors.lastname: Bashandy
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Guilleminot
authors.forename: Teva
authors.initials: T
authors.lastname: Vernoux
authors.forename: David
authors.initials: D
authors.lastname: Caparros-Ruiz
authors.forename: Karin
authors.initials: K
authors.lastname: Ljung
authors.forename: Yves
authors.initials: Y
authors.lastname: Meyer
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Reichheld
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D017343:Genes, Plant; D005978:Glutathione / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism*; D009154:Mutation; D009249:NADP / Q000378:metabolism*; D015398:Signal Transduction; D013879:Thioredoxins / Q000378:metabolism*
chemical_list: D007210:Indoleacetic Acids; D013879:Thioredoxins; D009249:NADP; D005978:Glutathione