Festuca arundinacea, glutathione S-transferase and herbicide safeners: a preliminary case study to reduce herbicidal pollution.
The expression of glutathione S-transferase (GST) activity in Festuca arundinacea was investigated in response to the following herbicide safeners: benoxacor, cloquintocet-mexyl, fenchlorazol-ethyl, fenclorim, fluxofenim and oxabetrinil. All the above compounds enhanced the GST activity tested towards the "model" substrate 1-chloro-2,4-dinitrobenzene (CDNB). Assays of GST activity towards the herbicides terbuthylazine (N(2)-tert-butyl-6-chloro-N(4)-ethyl-1,3,5-triazine-2,4-diamine) and butachlor (N-butoxymethyl-2-chloro-2',6'-diethylacetanilide) as substrates also showed the ability of the safeners to enhance the enzyme activity towards both these herbicides, with the exception of cloquintocet-mexyl for the enzyme activity towards butachlor. As a consequence of the above effects at a macro-scale level, decreased herbicide accumulation and persistence were ascertained in response to the addition of the safener benoxacor to both terbuthylazine and butachlor treatments. These results are discussed in terms of capacity of benoxacor to induce herbicide detoxification in Festuca arundinacea with a view to utilizing them in reducing herbicide pollution.
Scarponi
Luciano
L
Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Perugia, Perugia, Italy. scarponi@unipg.it
Del Buono
Daniele
D
D000083:Acetanilides / Q000494:pharmacology; D000097:Acetonitriles / Q000494:pharmacology; D000098:Acetophenones / Q000494:pharmacology; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004785:Environmental Pollutants / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D004790:Enzyme Induction / Q000187:drug effects; D031751:Festuca / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D006540:Herbicides / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D007097:Imines / Q000494:pharmacology; D010078:Oxazines / Q000494:pharmacology; D010091:Oximes / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D013997:Time Factors; D014227:Triazines / Q000494:pharmacology
D000083:Acetanilides; D000097:Acetonitriles; D000098:Acetophenones; D004137:Dinitrochlorobenzene; D004785:Environmental Pollutants; D006540:Herbicides; D007097:Imines; D010078:Oxazines; D010091:Oximes; D011743:Pyrimidines; D011804:Quinolines; D014227:Triazines; C514517:oxabetrinil; C501523:fenclorim; C108005:fluxofenim; C054409:butachlor; C514515:cloquintocet-mexyl; D005982:Glutathione Transferase; C037565:terbutylazine; C085163:benoxacor