Broad spectrum microarray for fingerprint-based bacterial species identification.
BACKGROUND
Microarrays are powerful tools for DNA-based molecular diagnostics and identification of pathogens. Most target a limited range of organisms and are based on only one or a very few genes for specific identification. Such microarrays are limited to organisms for which specific probes are available, and often have difficulty discriminating closely related taxa. We have developed an alternative broad-spectrum microarray that employs hybridisation fingerprints generated by high-density anonymous markers distributed over the entire genome for identification based on comparison to a reference database.


RESULTS
A high-density microarray carrying 95,000 unique 13-mer probes was designed. Optimized methods were developed to deliver reproducible hybridisation patterns that enabled confident discrimination of bacteria at the species, subspecies, and strain levels. High correlation coefficients were achieved between replicates. A sub-selection of 12,071 probes, determined by ANOVA and class prediction analysis, enabled the discrimination of all samples in our panel. Mismatch probe hybridisation was observed but was found to have no effect on the discriminatory capacity of our system.


CONCLUSIONS
These results indicate the potential of our genome chip for reliable identification of a wide range of bacterial taxa at the subspecies level without laborious prior sequencing and probe design. With its high resolution capacity, our proof-of-principle chip demonstrates great potential as a tool for molecular diagnostics of broad taxonomic groups.
Pasquer
Frédérique
F
Agroscope Changins-Wädenswil Research Station ACW, Laboratory for Molecular Diagnostics and Epidemiology, CH-8820 Wädenswil, Switzerland.
Pelludat
Cosima
C
Duffy
Brion
B
Frey
Jürg E
JE
D000704:Analysis of Variance; D001419:Bacteria / Q000145:classification / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D016680:Genome, Bacterial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial