A novel chemiluminescence method for the determination of ergometrine maleate in serum sample without chemiluminescence reagent.
In this paper, a novel flow injection-chemiluminescence (FI-CL) method was proposed for the determination of ergometrine maleate in serum. The new CL reaction was based on the direct oxidation of ergometrine maleate by the complex of metal chelate diperiodatocuprate(III) (K(5)[Cu(HIO(6))(2)]) in an alkaline medium. The CL intensity was enhanced in the presence of ascorbic acid. Hereby under the optimum conditions, ergometrine maleate was determined over the range of 4.0 x 10(-9) gm L(-1) to 4.0 x 10(-7) gm L(-1) with a limit of detection (3 sigma) of 1.1 x 10(-9) gm L(-1). The relative standard deviation (R.S.D.) was 2.1% for 8.0 x 10(-9) gm L(-1) ergometrine maleate (n=7). The sensitive method was successfully applied to the direct determination of ergometrine maleate (ng mL(-1)) in pharmaceutical injection and serum samples. The mechanism of the reactions was also discussed.
Hu
Yufei
Y
School of Chemistry and Chemical Engineering, Sun Yat-Sen University, 135 Xingang Xi Road, Guangzhou, Guangdong 510275, China.
Zhang
Zhujun
Z
Li
Gongke
G
D001205:Ascorbic Acid / Q000737:chemistry; D001774:Blood Chemical Analysis / Q000379:methods*; D002614:Chelating Agents / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004874:Ergonovine / Q000097:blood* / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods*; D010084:Oxidation-Reduction
D002614:Chelating Agents; D007202:Indicators and Reagents; D003300:Copper; D001205:Ascorbic Acid; D004874:Ergonovine