Optimization of supercritical fluid extraction of saikosaponins from Bupleurum falcatum with orthogonal array design.
Supercritical fluid extraction (SFE) was used to extract saikosaponins a, c and d from the root of Bupleurum falcatum. An orthogonal array design L(9)(3)(4) was employed as a chemometric method for the optimization of the SFE conditions. The effects of four factors including pressure (30-40 MPa), temperature (40-50 degrees C), ethanol concentration (60-100%) and time (2.5-3.5 h) on the yields of saikosaponins were investigated by a preparative SFE system in the SFE mode. Under the optimized conditions, namely 35 MPa of pressure, 45 degrees C of temperature, 80% of ethanol concentration and 3.0 h of time, the yields of saikosaponin c, saikosaponin a, saikosaponin d, total saikosaponins and SFE extract were 0.16, 0.12, 0.96, 1.24 and 16.48 mg/g, respectively. Determinations of the saikosaponins were performed by HPLC.
Sun
Yinshi
Y
State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, PR China.
Wei
Li
L
Wang
Jianhua
J
Bi
Jianjie
J
Liu
Zhengbo
Z
Wang
Ying
Y
Guo
Zude
Z
D029972:Bupleurum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D000431:Ethanol / Q000737:chemistry; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D011312:Pressure; D012503:Saponins / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors
D012503:Saponins; D000431:Ethanol; D009828:Oleanolic Acid; C025759:saikosaponin D