title: Bioactive steroidal alkaloids from Sarcococca hookeriana.
abstract: Four new 5 alpha-pregnane-type steroidal alkaloids, hookerianamides L(1), M(2), N(3), and O(4), and a known N-formylchonemorphine (5) have been isolated by acid-base extraction of the dichloromethane extract of Sarcococca hookeriana. The structures of all compounds were determined with spectroscopic techniques and by comparison with literature data. All compounds displayed antileishmanial and antibacterial properties. Compounds 1, 4, and 5 were found to be more potent than standard pentamidine (IC (50) = 9.59 microg/mL) with respect to leishmanicidal activity. The minimum inhibitory concentration of most of the compounds against Bacillus subtilis, Streptococcus minor, and Streptococcus ferus was lower than that of the standard ampicillin.
authors.affiliation: Institute of Forestry, Pokhara Campus, Tribhuvan University, Pokhara, Kaski, Nepal. devkotakpd@yahoo.com
authors.forename: Krishna Prasad
authors.initials: KP
authors.lastname: Devkota
authors.forename: Jean D
authors.initials: JD
authors.lastname: Wansi
authors.forename: Bruno N
authors.initials: BN
authors.lastname: Lenta
authors.forename: Samreen
authors.initials: S
authors.lastname: Khan
authors.forename: Muhammad I
authors.initials: MI
authors.lastname: Choudhary
authors.forename: Norbert
authors.initials: N
authors.lastname: Sewald
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D031234:Buxaceae / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010419:Pentamidine / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011278:Pregnanes / Q000302:isolation & purification / Q000494:pharmacology*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014344:Trypanocidal Agents / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D011278:Pregnanes; D013256:Steroids; D014344:Trypanocidal Agents; C054612:chonemorphine; D010419:Pentamidine; D000667:Ampicillin