title: Filopodial focal complexes direct adhesion and force generation towards filopodia outgrowth.
abstract: Filopodia are key structures within many cells that serve as sensors constantly probing the local environment. Although filopodia are involved in a number of different cellular processes, their function in migration is often analyzed with special focus on early processes of filopodia formation and the elucidation of filopodia molecular architecture. An increasing number of publications now describe the entire life cycle of filopodia, with analyses from the initial establishment of stable filopodium-substrate adhesion to their final integration into the approaching lamellipodium. We and others can now show the structural and functional dependence of lamellipodial focal adhesions as well as of force generation and transmission on filopodial focal complexes and filopodial actin bundles. These results were made possible by new high resolution imaging techniques as well as by recently developed elastomeric substrates and theoretical models. The data additionally provide strong evidence that formation of new filopodia depends on previously existing filopodia through a repetitive filopodial elongation of the stably adhered filopodial tips. In this commentary we therefore hypothesize a highly coordinated mechanism that regulates filopodia formation, adhesion, protein composition and force generation in a filopodia dependent step by step process.
authors.affiliation: Institute of Bio- and Nanosystems, IBN-4, Biomechanics, Research Centre Jülich, Jülich, Germany. b.hoffmann@fz-juelich.de
authors.forename: Bernd
authors.initials: B
authors.lastname: Hoffmann
authors.forename: Claudia
authors.initials: C
authors.lastname: Schäfer
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D022001:Focal Adhesions / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D011554:Pseudopodia / Q000378:metabolism*
chemical_list: D000199:Actins