title: Cholecystokinin as a regulator of cardiac function and postprandial gastrointestinal blood flow in rainbow trout (Oncorhynchus mykiss).
abstract: We have studied the potential role of CCK as a regulator/modulator of the postprandial increase in gastrointestinal blood flow. Rainbow trout (Oncorhynchus mykiss) were instrumented with pulsed Doppler flow probes to measure the effects of CCK on cardiac output and gastrointestinal blood flow. Furthermore, vascular preparations were used to study the direct effects of CCK on the vessels. In addition, we used in situ perfused hearts to further study the effects of CCK on the cardiovascular system. When the sulfated form of CCK-8 was injected at a physiological concentration (0.19 pmol/kg) in vivo, there was a significant increase in the gastrointestinal blood flow (18 +/- 4%). This increase in gastrointestinal blood flow was followed by a subsequent increase in cardiac output (30 +/- 6%). When the dose was increased to 0.76 pmol/kg, there was only a 14 +/- 6% increase in gastrointestinal blood flow; possibly due to a dose-dependent increase in the gill vascular resistance as previously reported or a direct effect on the heart. Nevertheless, CCK did not affect the isolated vessel preparations, and thus, it seems unlikely that CCK has a direct effect on the blood vessels of the second or third order. CCK did, however, have profound effects on the dynamics of the heart, and without a change in cardiac output, there was a significant increase in the amplitude (59 +/- 4%) and rate (dQ/dt: 55 +/- 4%; -dQ/dt: 208 +/- 49%) of the phasic flow profile. If and how this might be coupled to a postprandial gastrointestinal hyperemia remains to be determined. We conclude that CCK has the potential as a regulator of the postprandial gastrointestinal blood flow in fish and most likely has its effect by inducing a gastrointestinal hyperemia. The mechanism by which CCK acts is at present unknown.
authors.affiliation: Department of Zoology, University of Gothenburg, Gothenburg, Sweden. henrik.seth@zool.gu.se
authors.forename: Henrik
authors.initials: H
authors.lastname: Seth
authors.forename: Albin
authors.initials: A
authors.lastname: Gräns
authors.forename: Michael
authors.initials: M
authors.lastname: Axelsson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002302:Cardiac Output / Q000187:drug effects / Q000502:physiology*; D017124:Conserved Sequence; D003971:Diastole / Q000187:drug effects / Q000502:physiology; D005765:Gastrointestinal Agents / Q000378:metabolism / Q000494:pharmacology; D041981:Gastrointestinal Tract / Q000098:blood supply* / Q000502:physiology; D006940:Hyperemia / Q000139:chemically induced / Q000503:physiopathology; D017686:Oncorhynchus mykiss / Q000502:physiology*; D019518:Postprandial Period / Q000187:drug effects / Q000502:physiology; D012039:Regional Blood Flow / Q000187:drug effects / Q000502:physiology*; D012844:Sincalide / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D013599:Systole / Q000187:drug effects / Q000502:physiology
chemical_list: D005765:Gastrointestinal Agents; D012844:Sincalide