title: STIL, a peculiar molecule from styles, specifically dephosphorylates the pollen receptor kinase LePRK2 and stimulates pollen tube growth in vitro.
abstract: BACKGROUND
  LePRK1 and LePRK2 are two pollen receptor kinases localized to the plasma membrane, where they are present in a high molecular weight complex (LePRK complex). LePRK2 is phosphorylated in mature and germinated pollen, but is dephosphorylated when pollen membranes are incubated with tomato or tobacco style extracts.
  
  
  RESULTS
  Here we show that LePRK2 dephosphorylation is mediated by a heat-, acid-, base-, DTT- and protease-resistant component from tobacco styles. Using LePRK2 phosphorylation as a tracking assay for purification, style exudates were subjected to chloroform extraction, anionic exchange, and C18 reverse-phase chromatography columns. We finally obtained a single ~3,550 Da compound (as determined by UV-MALDI-TOF MS) that we named STIL (for Style Interactor for LePRKs). STIL increased pollen tube lengths of in vitro germinated pollen in a dose-dependent manner.
  
  
  CONCLUSIONS
  We propose that the LePRK complex perceives STIL, resulting in LePRK2 dephosphorylation and an increase in pollen tube growth.
authors.affiliation: Instituto de Ingeniería Genética y Biología Molecular, CONICET, Buenos Aires, Argentina.
authors.forename: Diego L
authors.initials: DL
authors.lastname: Wengier
authors.forename: María A
authors.initials: MA
authors.lastname: Mazzella
authors.forename: Tamara M
authors.initials: TM
authors.lastname: Salem
authors.forename: Sheila
authors.initials: S
authors.lastname: McCormick
authors.forename: Jorge P
authors.initials: JP
authors.lastname: Muschietti
mesh_terms: D018551:Solanum lycopersicum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010766:Phosphorylation; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D053205:Pollen Tube / Q000254:growth & development*; D011493:Protein Kinase C / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; C061570:protein kinase N; D011493:Protein Kinase C