Activity of an enzyme immobilized on superparamagnetic particles in a rotational magnetic field.
We immobilize alpha-amylase extracted from Bacillus Iicheniformis on the surfaces of superparamagnetic particles and investigate the effect of a rotational magnetic field on the enzyme's activity. We find that the activity of the enzyme molecules immobilized on superparamagnetic particles increases in the rotational magnetic field and reaches maximum at a certain frequency. We clarify the effect of the cluster structures formed by the superparamagnetic particles on the activity. Enzyme reactions are enhanced even in a tiny volume of solution using the present method, which is very important for the development of efficient micro reactors and micro total analysis systems (mu-TAS).
Mizuki
Toru
T
Bio-Nano Electronics Research Centre, Toyo University, Saitama 350-8585, Japan.
Watanabe
Noriyuki
N
Nagaoka
Yutaka
Y
Fukushima
Tadamasa
T
Morimoto
Hisao
H
Usami
Ron
R
Maekawa
Toru
T
D004574:Electromagnetic Fields; D004800:Enzymes, Immobilized / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011487:Protein Conformation; D012399:Rotation; D000516:alpha-Amylases / Q000737:chemistry*
D004800:Enzymes, Immobilized; D000516:alpha-Amylases; C501919:alpha-amylase, Bacillus licheniformis; D052203:Ferrosoferric Oxide