Rapid and reliable single nucleotide polymorphism-based differentiation of Brucella live vaccine strains from field strains.
The reliable differentiation of live Brucella vaccine strains from field isolates is an important element in brucellosis control programs. We describe the design, validation, and implementation of a novel single nucleotide polymorphism (SNP)-based typing platform that offers a rapid, reliable, and robust tool to achieve this with improved diagnostic accuracy compared to existing molecular tests. Furthermore, the assays described are designed such that they supplement, and can be run as an intrinsic part of, a previously described assay identifying Brucella isolates to the species level (K. K. Gopaul, C. J. Smith, M. S. Koylass, and A. M. Whatmore, BMC Microbiol. 8:86), giving a comprehensive molecular typing platform.
Gopaul
Krishna K
KK
Department of Statutory and Exotic Bacterial Disease, Veterinary Laboratories Agency, Woodham Lane, New Haw, United Kingdom.
Sells
Jessica
J
Bricker
Betsy J
BJ
Crasta
Oswald R
OR
Whatmore
Adrian M
AM
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D002002:Brucella / Q000145:classification* / Q000235:genetics*; D002004:Brucella Vaccine / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide
D002004:Brucella Vaccine; D004269:DNA, Bacterial