Imaging of RNA delivery to cells by thiazole orange as a fluorescent RNA base substitution.
Interstrand thiazole orange (TO) dimers in RNA show a yellow colored emission that can be distinguished from the green TO monomer emission by confocal microscopy inside CHO cells.
Berndl
Sina
S
Institute for Organic Chemistry, University of Regensburg, 93040 Regensburg, Germany.
Breunig
Miriam
M
Göpferich
Achim
A
Wagenknecht
Hans-Achim
HA
D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005456:Fluorescent Dyes / Q000737:chemistry*; D008055:Lipids; D018613:Microscopy, Confocal / Q000379:methods*; D011804:Quinolines / Q000737:chemistry*; D012313:RNA / Q000008:administration & dosage* / Q000032:analysis* / Q000737:chemistry
D052160:Benzothiazoles; D005456:Fluorescent Dyes; D008055:Lipids; C086724:Lipofectamine; D011804:Quinolines; C051000:thiazole orange; D012313:RNA