title: TNF-alpha-induced aquaporin 9 in synoviocytes from patients with OA and RA.
abstract: OBJECTIVE
  To determine whether aquaporins (AQPs) are expressed in the synovial tissues of patients with OA and RA, and to examine the patterns of expression in patients with and without hydrarthrosis.
  
  
  METHODS
  AQPs were detected in synovial tissue samples from patients with OA and RA using RT-PCR and immunohistochemistry. Fibroblast-like synoviocytes (FLSs) from patients with OA and RA were cultured and stimulated with TNF-alpha. The expression of AQPs in FLSs was examined using RT-PCR and western blot analyses and the function of aquaglyceroporins was examined by a glycerol uptake assay.
  
  
  RESULTS
  AQP1, -3 and -9 mRNAs were expressed in synovial tissues from patients with OA and RA. AQP1, -3 and -9 proteins were also detected by immunohistochemistry. AQP9 mRNA was expressed more strongly in the synovial tissues of OA patients with hydrarthrosis than those without. AQP9 mRNA and protein expression were strongly induced with TNF-alpha treatment in FLSs, whereas the expression of AQP1 and -3 mRNAs was not induced with TNF-alpha treatment. AQP9 as an aquaglyceroporin was induced by TNF-alpha.
  
  
  CONCLUSIONS
  AQP9 mRNA was detected in synovial tissues from OA and RA patients with hydrarthrosis. AQP9 expression was strongly induced in FLSs with TNF-alpha. Although the functions of AQP1, -3 and -9 in synovial tissues remain to be elucidated, it suggested that AQP9 might be related to the pathogenesis of hydrarthrosis and inflammatory synovitis.
authors.affiliation: Department of Orthopaedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
authors.forename: Masashizu
authors.initials: M
authors.lastname: Nagahara
authors.forename: Yuko
authors.initials: Y
authors.lastname: Waguri-Nagaya
authors.forename: Takaya
authors.initials: T
authors.lastname: Yamagami
authors.forename: Mineyoshi
authors.initials: M
authors.lastname: Aoyama
authors.forename: Toyohiro
authors.initials: T
authors.lastname: Tada
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Inoue
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Asai
authors.forename: Takanobu
authors.initials: T
authors.lastname: Otsuka
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D020346:Aquaporins / Q000187:drug effects*; D001168:Arthritis / Q000378:metabolism* / Q000473:pathology; D001172:Arthritis, Rheumatoid / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D006833:Hydrarthrosis / Q000378:metabolism* / Q000473:pathology; D007719:Knee Joint; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism / Q000473:pathology; D013583:Synovial Membrane / Q000378:metabolism*; D013585:Synovitis / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: C109669:AQP9 protein, human; D020346:Aquaporins; D014409:Tumor Necrosis Factor-alpha