title: The study on the role of inflammatory cells and mediators in post-infectious functional dyspepsia.
abstract: OBJECTIVE
  Functional dyspepsia is a common gastrointestinal disorder. The pathogenesis of functional dyspepsia remains unclear. Functional dyspepsia may begin after a bout of gastroenteritis (post-infectious functional dyspepsia) or de novo (nonspecific functional dyspepsia). The aim of this study was to investigate the prevalence and probable mechanisms of post-infectious functional dyspepsia.
  
  
  METHODS
  Functional dyspepsia patients with a history of unsanitary food intake and acute gastroenteritis 6-12 months ago were enrolled. (13)C-UBT confirmed absence of H. pylori infection. Controls consisted of healthy nondyspeptic volunteers and patients with nonspecific functional dyspepsia. Gastric biopsies were used for routine histology, immunohistochemistry, electron microscopy, ELISA, HPLC assays and Western blot examination.
  
  
  RESULTS
  Eighty-five subjects were entered including 35 with post-infectious functional dyspepsia, 30 with nonspecific functional dyspepsia, and 20 healthy controls. The number of mast cells in post-infectious functional dyspepsia and nonspecific functional dyspepsia were significantly greater than that in healthy controls. The number of enterochromaffin cells (ECs) in post-infectious functional dyspepsia was significantly higher than those in nonspecific functional dyspepsia or in healthy controls. The number of mast cells and ECs increased with the density of chronic inflammatory cells. The release of histamine and 5-hydroxytryptamine from gastric mucosa of post-infectious functional dyspepsia patients was significantly greater than those from nonspecific functional dyspepsia or healthy controls. Tryptase protein expression was higher in post-infectious functional dyspepsia and nonspecific functional dyspepsia than in healthy controls. The histological score of chronic gastric inflammation was greater in post-infectious functional dyspepsia versus patients with nonspecific functional dyspepsia or healthy controls. Electron microscopy showed secreting granules in the cytoplasm of both mast cells and ECs. The number of activated mast cells and Ecs at a distance of < 5 microm of nerve fibers were significantly greater in post-infectious functional dyspepsia versus nonspecific functional dyspepsia or controls.
  
  
  CONCLUSIONS
  Dyspepsia may occur after an acute onset of gastroenteritis in a part of patients. Potent chemicals derived from mast cells and ECs, including histamine, tryptase and 5-hydroxytryptamine may be involved in the pathogenesis of post-infectious functional dyspepsia.
authors.affiliation: Department of Gastroenterology, Shanghai Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Institute of Digestive Disease, Shanghai, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Li
authors.forename: Huimin
authors.initials: H
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.forename: Weiguang
authors.initials: W
authors.lastname: Li
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Chen
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Peng
authors.forename: Zhizheng
authors.initials: Z
authors.lastname: Ge
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D001706:Biopsy; D015153:Blotting, Western; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002851:Chromatography, High Pressure Liquid; D004415:Dyspepsia / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D016099:Endoscopy, Gastrointestinal; D004759:Enterochromaffin Cells / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005759:Gastroenteritis / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D006632:Histamine / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000473:pathology*; D008854:Microscopy, Electron; D008875:Middle Aged; D015995:Prevalence; D012701:Serotonin / Q000378:metabolism; D018709:Statistics, Nonparametric; D011795:Surveys and Questionnaires; D053802:Tryptases / Q000378:metabolism
chemical_list: D012701:Serotonin; D006632:Histamine; D053802:Tryptases