Real time imaging of femtosecond laser induced nano-neurosurgery dynamics in C. elegans.
In this study we present for the first time the use of confocal microscopy and laser scanning brightfield microscopy (LSBF) for real time imaging of femtosecond laser nanosurgery and its dynamics in C. elegans. A single multimodal optical workstation that provides the ability to perform femtosecond laser nanosurgery and simultaneous confocal and LSBF imaging was used for the purpose. With this tool several dynamic phenomena concomitant with laser nanosurgery in C. elegans were observed and imaged. Some of these dynamic phenomena, like muscular contraction and single muscle cell stimulation, have been imaged for the first time during nano-neurosurgery of C. elegans.
Santos
Susana I C O
SI
ICFO-Institut de Ciències Fotòniques, Mediterranean Technology Park, 08860, Castelldefels (Barcelona), Spain.
Mathew
Manoj
M
Loza-Alvarez
Pablo
P
D000818:Animals; D017173:Caenorhabditis elegans; D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D053685:Laser Therapy / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008866:Microsurgery / Q000295:instrumentation*; D009046:Motor Neurons; D036103:Nanotechnology / Q000295:instrumentation; D019635:Neurosurgical Procedures / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013116:Spinal Cord / Q000166:cytology* / Q000601:surgery*; D025321:Surgery, Computer-Assisted / Q000295:instrumentation*