title: Derivation and maintenance of human embryonic stem cell line on human adult skin fibroblast feeder cells in serum replacement medium.
abstract: Human embryonic stem (hES) cells were originally isolated and maintained on mouse embryonic fibroblast (MEF) feeder layers in the presence of fetal bovine serum (FBS). However, if the hES cells are to be used for therapeutic applications, it is preferable to regulatory authorities that they be derived and cultured in animal-free conditions to prevent mouse antigen contamination that would exacerbate an immune response to foreign proteins, and the potential risk of transmission of retroviral and other zoonotic pathogens to humans. As a step towards this goal, we derived a new hES cell line (MISCES-01) on human adult skin fibroblasts as feeder cells using serum replacement (SR) medium. The MISCES-01 cells have a normal diploid karyotype (46XX), express markers of pluripotency (OCT4, GCTM-2, TRA-1-60, TRA-1-81, SSEA-3, SSEA-4, and alkaline phosphatase) and following in vitro and in vivo differentiation, give rise to derivatives of the three primary germ layers. This cell line can be obtained for research purposes from the Australian Stem Cell Centre (http://www.stemcellcentre.edu.au).
authors.affiliation: Research Office, Monash University, Clayton, VIC 3800, Australia.
authors.forename: R Tayfur
authors.initials: RT
authors.lastname: Tecirlioglu
authors.forename: Linh
authors.initials: L
authors.lastname: Nguyen
authors.forename: Karen
authors.initials: K
authors.lastname: Koh
authors.forename: Alan O
authors.initials: AO
authors.lastname: Trounson
authors.forename: Anna E
authors.initials: AE
authors.lastname: Michalska
mesh_terms: D000328:Adult; D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D051379:Mice; D044967:Serum; D012867:Skin / Q000166:cytology*
chemical_list: D003470:Culture Media