title: Polymorphisms of genes encoding tumor necrosis factor-alpha, interleukin-10, cluster of differentiation-14 and interleukin-1ra in critically ill patients.
abstract: OBJECTIVE
  The aim of the study was to determine whether distributions of tumor necrosis factor (TNF)-α(308), interleukin (IL)-10(1082), CD14(159), and IL-1ra gene intron 2 genotypes in critically ill patients are associated with outcome, underlying cause of sepsis, and type of microorganism.
  
  
  METHODS
  Blood samples from 106 critically ill white patients were genotyped by method based on polymerase chain reaction for TNF-α(308), IL-10(1082), CD14(159), and IL-1ra gene intron 2.
  
  
  RESULTS
  All patients with TNF-α(308)AA genotype survived; relative risk (RR) of death in patients with AG was 3.250 and with GG, 1.923 (P < .01). In patients with Gram-positive sepsis, IL-10(1082)AA and then AG genotypes were the most frequent ones (odds ratio [OR], 18.67 and 7.20, respectively; P < .01). When comparing IL-10(1082)AA with AG, RR of pancreatitis was 1.80 and OR was 3.40. When AA and GG were compared, RR was 7.33 and OR was 20.00. In patients with GG, RR of peritonitis was 4.07 and OR was 5.88 (P < .01). In patients with Gram-positive sepsis, CD14(159)CT was the most frequent one with OR of 5.25. Distribution of 6 IL-1ra gene intron 2 genotypes showed no significant association.
  
  
  CONCLUSIONS
  Distribution of TNF-α(308) genotypes is associated with outcome, IL-10(1082) with type of microorganism and underlying cause of sepsis, and CD14(159) with type of microorganism.
authors.affiliation: Clinic of Anesthesiology and Intensive Therapy, Military Medical Academy, 11000 Belgrade, Serbia. maya@eunet.rs
authors.forename: Maja
authors.initials: M
authors.lastname: Surbatovic
authors.forename: Krasimirka
authors.initials: K
authors.lastname: Grujic
authors.forename: Bojana
authors.initials: B
authors.lastname: Cikota
authors.forename: Miodrag
authors.initials: M
authors.lastname: Jevtic
authors.forename: Nikola
authors.initials: N
authors.lastname: Filipovic
authors.forename: Predrag
authors.initials: P
authors.lastname: Romic
authors.forename: Natasa
authors.initials: N
authors.lastname: Strelic
authors.forename: Zvonko
authors.initials: Z
authors.lastname: Magic
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016638:Critical Illness; D005260:Female; D005838:Genotype; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D018805:Sepsis / Q000235:genetics* / Q000382:microbiology / Q000401:mortality; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014947:Wounds and Injuries / Q000235:genetics / Q000382:microbiology / Q000401:mortality; D055815:Young Adult
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D018950:Lipopolysaccharide Receptors; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10