Analysis of perchlorate in milk powder and milk by hydrophilic interaction chromatography combined with tandem mass spectrometry.
A simple, selective, and sensitive method using hydrophilic interaction chromatography combined with tandem mass spectrometry (HILIC-MS/MS) for quantifying perchlorate in milk powder and milk was developed. The analysis was conducted on an Inertsil HILIC column (150 mm x 3.0 mm, 3.5 mum) using a mobile phase consisting of methanol and 0.1% formic acid (60:40, v/v). The detection was performed by MS/MS via electrospray ionization. Linear calibration curves were obtained in the concentration range of 2.00 x 10(-2) to 8.00 microg/g and 4.00 x 10(-1) to 20.0 microg/L for perchlorate in milk powder and milk, respectively. The method detection limit was 4.00 x 10(-3) microg/g for milk powder and 8.00 x 10(-2) microg/L for milk. The recoveries of perchlorate in milk powder and milk were all >90%. This method was successfully applied to the quantitative determination of perchlorate in milk powder and milk.
Chen
Lingyun
L
Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, RP China.
Chen
Huashan
H
Shen
Mei
M
Zhou
Zhifeng
Z
Ma
Ande
A
D000818:Animals; D002417:Cattle; D002845:Chromatography / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D010472:Perchlorates / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D010472:Perchlorates; C494474:perchlorate