Drosophila melanogaster larvae as a model for blast lung injury.
BACKGROUND
Primary blast injuries, specifically lung injuries, resulting from blast overpressure exposures are a major source of mortality for victims of blast events. However, existing pulmonary injury criteria are inappropriate for common exposure environments. This study uses Drosophila melanogaster larvae to develop a simple phenomenological model for human pulmonary injury from primary blast exposure.


METHODS
Drosophila larvae were exposed to blast overpressures generated by a 5.1-cm internal diameter shock tube and their mortality was observed after the exposure. To establish mortality thresholds, a survival analysis was conducted using survival data and peak incident pressures. In addition, a histologic analysis was performed on the larvae to establish the mechanisms of blast injury.


RESULTS
The results of the survival analysis suggest that blast overpressure for 50% Drosophila survival is greater than human threshold lung injury and is similar to human 50% survival levels, in the range of overpressure durations tested (1-5 ms). A "parallel" analysis of the Bass et al. 50% human survival curves indicates that 50% Drosophila survival is equivalent to a human injury resulting in a 69% chance of survival. Histologic analysis of the blast-exposed larvae failed to demonstrate damage to the dorsal trunk of the tracheal system; however, the presence of flocculent material in the larvae body cavities and tracheas suggests tissue damage.


CONCLUSIONS
This study shows that D. melanogaster survival can be correlated with large animal injury models to approximate a human blast lung injury tolerance. Within the range of durations tested, Drosophila larvae may be used as a simple model for blast injury.
Bass
Cameron R
CR
Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Meyerhoff
Kevin P
KP
Damon
Andrew M
AM
Bellizzi
Andrew M
AM
Salzar
Robert S
RS
Rafaels
Karin A
KA
D055371:Acute Lung Injury / Q000473:pathology*; D000818:Animals; D001753:Blast Injuries / Q000473:pathology*; D004195:Disease Models, Animal; D004331:Drosophila melanogaster / Q000033:anatomy & histology; D019220:High-Energy Shock Waves; D006801:Humans; D007814:Larva / Q000033:anatomy & histology; D008168:Lung / Q000473:pathology