title: Activity of siRNAs with 2-thio-2'-O-methyluridine modification in mammalian cells.
abstract: In a search to identify chemical modifications to improve the properties of siRNA, we have investigated the effect of the 2 '-O-methyl-2-thiouridine modification on the biological activity of siRNA. Our results indicate that judicious placement of 2 '-O-methyl-2-thiouridine residues could lead to modified siRNA with activity in mammalian cells.
authors.affiliation: Department of Medicinal Chemistry, Isis Pharmaceuticals Inc., Carlsbad, California 92008, USA. tprakashn@isisph.com
authors.forename: Thazha P
authors.initials: TP
authors.lastname: Prakash
authors.forename: Nishant
authors.initials: N
authors.lastname: Naik
authors.forename: Namir
authors.initials: N
authors.lastname: Sioufi
authors.forename: Balkrishen
authors.initials: B
authors.lastname: Bhat
authors.forename: Eric E
authors.initials: EE
authors.lastname: Swayze
mesh_terms: D002478:Cells, Cultured; D015195:Drug Design; D006367:HeLa Cells; D006801:Humans; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism
chemical_list: C409967:2'-O-methyl-2-thiouridine; D009841:Oligonucleotides; D034741:RNA, Small Interfering; D013891:Thiouridine