title: Dual-subtype feline immunodeficiency virus vaccine provides 12 months of protective immunity against heterologous challenge.
abstract: The duration of immunity of the dual-subtype feline immunodeficiency virus (FIV) vaccine, Fel-O-Vax FIV, for protection against subtype-B FIV was assessed in this study. Vaccinated cats along with controls were challenged with FIV(FC1), a subtype-B FIV strain, 54 weeks after the final vaccination, and monitored for 46-48 weeks for provirus and viral RNA in peripheral blood, provirus in lymphoid organs, and CD4:CD8 ratios. Results of provirus detection in peripheral blood and lymphoid organs and plasma viral RNA loads showed that 10/14 vaccinated cats were fully protected for 48 weeks against infection with FIV(FC1) whereas 5/5 controls were persistently infected with FIV(FC1). CD4:CD8 inversions were noted in association with FIV infection and viral loads were not significantly different between FIV infected controls and the unprotected vaccinated animals.
authors.affiliation: Fort Dodge Animal Health Division of Wyeth, Fort Dodge, IA 50501, USA. chengjin.huang@boehringer-ingelheim.com
authors.forename: Chengjin
authors.initials: C
authors.lastname: Huang
authors.forename: Douglas
authors.initials: D
authors.lastname: Conlee
authors.forename: Michael
authors.initials: M
authors.lastname: Gill
authors.forename: Hsien-Jue Steve
authors.initials: HJ
authors.lastname: Chu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016516:CD4-CD8 Ratio / Q000662:veterinary; D002371:Cat Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D002415:Cats; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D037901:Euthanasia, Animal; D005260:Female; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D016180:Lentivirus Infections / Q000517:prevention & control / Q000662:veterinary*; D008198:Lymph Nodes / Q000821:virology; D008297:Male; D011533:Proviruses / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D012367:RNA, Viral / Q000097:blood; D013047:Specific Pathogen-Free Organisms; D019562:Viral Load / Q000662:veterinary; D014765:Viral Vaccines
chemical_list: D000914:Antibodies, Viral; D017931:DNA Primers; D012367:RNA, Viral; D014765:Viral Vaccines