title: Monitoring toxic microalgae Ostreopsis (dinoflagellate) species in coastal waters of the Mediterranean Sea using molecular PCR-based assay combined with light microscopy.
abstract: A molecular PCR-based assay was developed and applied to macrophyte and seawater samples containing mixed microphytobenthic and phytoplanktonic assemblages, respectively, in order to detect toxic Ostreopsis species in Mediterranean Sea. The specificity and sensitivity of the molecular PCR assay were assessed with both plasmidic and genomic DNA of the target genus or species using taxon-specific primers in the presence of background macrophyte DNA. The PCR molecular technique allowed rapid detection of the Ostreopsis cells, even at abundances undetectable within the resolution limit of the microscopy technique. Species-specific identification of Ostreopsis was determined only by PCR-based assay, due to the inherent difficulty of morphological identification in field samples. In the monitoring of the toxic Ostreopsis blooms PCR-based methods proved to be effective tools complementary to microscopy for rapid and specific detection of Ostreopsis and other toxic dinoflagellates in marine coastal environments.
authors.affiliation: Dipartimento di Scienze Biomolecolari, Sez. Biol. Amb., Università di Urbino, Viale Trieste 296, 61100 Pesaro, Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Battocchi
authors.forename: Cecilia
authors.initials: C
authors.lastname: Totti
authors.forename: Magda
authors.initials: M
authors.lastname: Vila
authors.forename: Mercedes
authors.initials: M
authors.lastname: Masó
authors.forename: Samuela
authors.initials: S
authors.lastname: Capellacci
authors.forename: Stefano
authors.initials: S
authors.lastname: Accoroni
authors.forename: Albert
authors.initials: A
authors.lastname: Reñé
authors.forename: Michele
authors.initials: M
authors.lastname: Scardi
authors.forename: Antonella
authors.initials: A
authors.lastname: Penna
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis / Q000235:genetics; D004141:Dinoflagellida / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D054750:Ecotoxicology / Q000379:methods*; D016678:Genome / Q000235:genetics; D005843:Geography; D008522:Mediterranean Sea; D008853:Microscopy; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012621:Seasons; D012623:Seawater; D013045:Species Specificity; D013696:Temperature; D013997:Time Factors
chemical_list: D004247:DNA