Data mining cDNAs reveals three new single stranded RNA viruses in Nasonia (Hymenoptera: Pteromalidae).
We report three novel small RNA viruses uncovered from cDNA libraries from parasitoid wasps in the genus Nasonia. The genome of this kind of virus is a positive-sense single-stranded RNA with a 3' poly(A), which facilitates cloning from cDNAs. Two of the viruses, NvitV-1 and NvitV-2, possess a RNA-dependent RNA polymerase that associates them with the family Iflaviridae of the order Picornavirales. A third virus, NvitV-3, is most similar to the Nora virus from Drosophila. A reverse transcription-PCR method developed for NvitV-1 indicates that it is a persistent commensal infection of Nasonia.
Oliveira
D C S G
DC
Department of Biology, University of Rochester, Rochester, NY 14627, USA.
Hunter
W B
WB
Ng
J
J
Desjardins
C A
CA
Dang
P M
PM
Werren
J H
JH
D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D057225:Data Mining; D020224:Expressed Sequence Tags; D015723:Gene Library; D008969:Molecular Sequence Data; D010802:Phylogeny; D012328:RNA Viruses / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014863:Wasps / Q000821:virology*