Purification of the Staphylococcus aureus bacteriophages VDX-10 on methacrylate monoliths.
Bacteriophages (phages) are known to be useful in many fields from medicine to agriculture, and for a broad range of applications, including phage therapy and phage display. For some applications, especially in medicine, high purity and viability of phages are required. Methacrylate monoliths (Convective Interaction Media [CIM] monolithic columns), designed for purification of bionanoparticles, were applied for the purification of Staphylococcus aureus phages VDX-10 from bacterial lysate. With a single step purification method, more than 99% of host cell DNA and more than 90% of proteins were removed, with 60% recovery of viable phages. Comparable results were obtained when the purification method was scaled-up from a CIM monolithic disk to a larger CIM monolithic column. Additionally, the dynamic binding capacity of a methacrylate monolith column for S. aureus phages VDX-10 was determined.
Kramberger
Petra
P
BIA Separations, d.o.o., Teslova 30, SI-1000 Ljubljana, Slovenia. petra.kramberger@monoliths.com
Honour
Richard C
RC
Herman
Richard E
RE
Smrekar
Franci
F
Peterka
Matjaz
M
D002852:Chromatography, Ion Exchange / Q000379:methods; D008689:Methacrylates; D013204:Staphylococcus Phages / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000821:virology*; D014773:Virology / Q000379:methods*
D008689:Methacrylates