Five-dimensional crystallography.
A method for determining a comprehensive chemical kinetic mechanism in macromolecular reactions is presented. The method is based on five-dimensional crystallography, where, in addition to space and time, temperature is also taken into consideration and an analysis based on singular value decomposition is applied. First results of such a time-resolved crystallographic study are presented. Temperature-dependent time-resolved X-ray diffraction measurements were conducted on the newly upgraded BioCARS 14-ID-B beamline at the Advanced Photon Source and aimed at elucidating a comprehensive kinetic mechanism of the photoactive yellow protein photocycle. Extensive time series of crystallographic data were collected at two temperatures, 293 K and 303 K. Relaxation times of the reaction extracted from these time series exhibit measurable differences for the two temperatures, hence demonstrating that five-dimensional crystallography is feasible.
Schmidt
Marius
M
Physics Department, University of Wisconsin-Milwaukee, Milwaukee, WI, USA. m-schmidt@uwm.edu
Graber
Tim
T
Henning
Robert
R
Srajer
Vukica
V
D003198:Computer Simulation; D018360:Crystallography, X-Ray; D007700:Kinetics; D046911:Macromolecular Substances / Q000737:chemistry*; D015394:Molecular Structure; D013997:Time Factors; D014961:X-Ray Diffraction
D046911:Macromolecular Substances