The structure of the 5'-untranslated region of mammalian poly(A) polymerase-alpha mRNA suggests a mechanism of translational regulation.
Poly(A)polymerase-alpha (PAPOLA) has been the most extensively investigated mammalian polyadenylating enzyme, mainly in regard to its multifaceted post-translational regulation. The possibility of translational regulation of this enzyme was addressed. The transcription start site was mapped and two uORFs, highly conserved among several species, were identified in the 211-bp long, GC-rich, 5' UTR of the PAPOLA mRNA. Mutation of the 5' proximal AUG resulted in increased translational efficiency of the adjacent coding sequence, whereas no significant effect was observed after mutation of the second AUG. These observations imply that translational regulation is among the conserved mechanisms regulating PAPOLA expression.
Rapti
Aikaterini
A
Papanikolaou Research Center of Oncology, St Savvas Hospital, Athens, Greece.
Trangas
Theoni
T
Samiotaki
Martina
M
Ioannidis
Panayotis
P
Dimitriadis
Euthymios
E
Meristoudis
Christos
C
Veletza
Stavroula
S
Courtis
Nelly
N
D020121:5' Untranslated Regions; D001483:Base Sequence; D002460:Cell Line; D017124:Conserved Sequence; D005091:Exons; D020862:GC Rich Sequence; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D011062:Polynucleotide Adenylyltransferase / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D014162:Transfection
D020121:5' Untranslated Regions; D012333:RNA, Messenger; D011062:Polynucleotide Adenylyltransferase