title: Interferon-gamma secretion and proliferative responses of peripheral blood mononuclear cells after vaccination of pigs against Aujeszky's disease in the presence of maternal immunity.
abstract: The development of T-cell responses in pigs vaccinated against Aujeszky's disease in the presence of maternal-derived antibodies (MDA) was examined. The aim of study was to evaluate the influence of MDA on the postvaccinal T-cell responses and optimization vaccination protocols in MDA-positive pigs. Pigs born to immune sows were vaccinated at different ages against Aujeszky's disease virus (ADV). For estimation of T-cell responses the lymphocyte proliferation and interferon (IFN)-gamma and interleukin-4 production were evaluated. High values of stimulation index were noted in groups vaccinated at 8 or 12 weeks of age (in 60% and 100% animals, respectively). In weaners vaccinated at 10 and 14 weeks of age, as well as in those vaccinated at 7 days and revaccinated at 8 or 12 weeks of age, 100% of animals positively responded in the lymphocyte proliferation assay after booster. At 20 weeks of life, only animals vaccinated at 12 weeks of age, 7 days and 12 weeks of age, and 10 and 14 weeks of age showed antigen-specific proliferation. Similar results were observed with IFN-gamma secretion after exposure to live ADV. We demonstrate that early vaccination with a live glycoprotein E-deleted ADV vaccine, in the face of high levels of MDA, could be effective, but the intensity and duration of the anamnestic response depends on the time of booster injection.
authors.affiliation: Department of Swine Diseases, National Veterinary Research Institute, Pulawy, Poland. mpomorska@piwet.pulawy.pl <mpomorska@piwet.pulawy.pl>
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pomorska-Mól
authors.forename: Iwona
authors.initials: I
authors.lastname: Markowska-Daniel
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D005260:Female; D011558:Herpesvirus 1, Suid / Q000276:immunology; D007112:Immunity, Maternally-Acquired / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000276:immunology; D011247:Pregnancy; D011557:Pseudorabies / Q000276:immunology* / Q000517:prevention & control; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines; D007371:Interferon-gamma