A comparison of in situ and in vitro techniques for bursting pressure testing of canine jejunum.
OBJECTIVE
To compare bursting pressures in canine jejunum, measured by use of an in vitro and an in situ bursting pressure technique.


METHODS
Cadavers of 3 healthy adult dogs.


METHODS
54 enterotomies were performed on 3 canine cadavers immediately after euthanasia. After completion of enterotomy closure, bursting pressure was measured on 9 jejunal segments by use of an in situ technique and on 9 jejunal segments by use of an in vitro technique for each canine cadaver. Bursting pressure testing time was recorded for both in situ and in vitro techniques. Techniques were compared by means of randomized block ANOVA.


RESULTS
The mean +/- SE in vitro and in situ bursting pressures were 93.63 +/- 24.10 mm Hg and 141.19 +/- 38.10 mm Hg and were not significantly different. Mean in situ testing time was 40.7 min/cadaver; mean in vitro testing time was 50.3 min/cadaver.


CONCLUSIONS
The in situ bursting pressure testing technique yielded results similar to those of the in vitro method, was somewhat less labor-intensive, and may be applicable to future studies of live dogs.
Curran
Kaitlin M
KM
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6610, USA.
Fransson
Boel A
BA
Gay
John M
JM
D000818:Animals; D002102:Cadaver; D004285:Dogs; D005260:Female; D007583:Jejunum / Q000502:physiology* / Q000601:surgery; D008297:Male; D009919:Orchiectomy; D010052:Ovariectomy; D011312:Pressure