title: Risk and prevention of bovine viral diarrhea virus (BVDV) transmission through embryo production via somatic cell nuclear transfer (SCNT) using oocytes from persistently infected donors.
abstract: The objective was to assess the risk of transmission of bovine viral diarrhea virus (BVDV) through embryo production via somatic cell nuclear transfer (SCNT), with oocytes obtained from persistently infected (PI) donors. Using ultrasound-guided follicular aspiration following superstimulation, oocytes were obtained from five female beef cattle, including three that were PI and two that were negative for BVDV. In the three PI cattle, seven aspirations yielded 32 oocytes (PI-1: three aspirations yielding six oocytes; PI-2: two aspirations yielding 14 oocytes; and PI-3: two aspirations yielding 12 oocytes). The oocyte recovery rate was better in negative control cattle, with 32 oocytes obtained from the two cattle in a single superstimulation and aspiration session. Oocytes were processed individually for SCNT, evaluated, and tested for BVDV. Nearly all (31/32) oocytes from the three PI donors were positive for BVDV by PCR, with detected viral RNA copy number ranging from 1 to 1.1 x 10(5). The proportion of oocytes acceptable for SCNT embryo production (based on oocyte quality and maturation status) was only 16 to 35% from PI donors, but was 81% from control donors. Therefore, routine testing of unacceptable (discarded) oocytes could be an effective approach to identify batches that might contain infected oocytes from PI donors. Identification and removal of high-risk batches of oocytes would minimize the risk of BVDV transmission through SCNT embryo production.
authors.affiliation: Viagen, Inc., Suite 100, Austin, TX 78727, USA. Keqin.gregg@viagen.com
authors.forename: K
authors.initials: K
authors.lastname: Gregg
authors.forename: K P
authors.initials: KP
authors.lastname: Riddell
authors.forename: S H
authors.initials: SH
authors.lastname: Chen
authors.forename: P K
authors.initials: PK
authors.lastname: Galik
authors.forename: T
authors.initials: T
authors.lastname: Xiang
authors.forename: T
authors.initials: T
authors.lastname: Guerra
authors.forename: M S
authors.initials: MS
authors.lastname: Marley
authors.forename: I
authors.initials: I
authors.lastname: Polejaeva
authors.forename: M D
authors.initials: MD
authors.lastname: Givens
mesh_terms: D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000517:prevention & control* / Q000635:transmission*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics; D005260:Female; D015571:Follicular Fluid / Q000821:virology; D053652:Nuclear Transfer Techniques; D018587:Oocyte Donation / Q000662:veterinary; D009865:Oocytes / Q000821:virology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000097:blood; D012307:Risk Factors; D020858:Tissue and Organ Harvesting / Q000379:methods / Q000662:veterinary
chemical_list: D012367:RNA, Viral