Study on the effect of T-2 toxin combined with low nutrition diet on rat epiphyseal plate growth and development.
The purpose of this study was to observe early lesions of rat epiphyseal plates and metaphysis caused by T-2 toxin and T-2 toxin combined with a low nutrition diet to determine possible pathogenic factors of Kashin-Beck disease (KBD). Ninety Wistar rats were divided into three groups. Group A was fed with a normal diet as control; group B was fed with a normal diet and T-2 toxin; and group C was fed with a low nutrition diet and T-2 toxin. The left knee specimens were collected, fixed in formaldehyde solution, stained by hematoxylin and eosin and Masson. After two weeks, the epiphyseal plate showed necrosis of chondrocytes in groups B and C. After four weeks, more obvious chondrocyte necrosis appeared. The positive rate of Lamellar necrosis in group C was significantly higher than that in groups B and A (P < 0.01). Metaphyseal trabecular bone showed sparse disorder and disruption in group C. T-2 toxin combined with a low nutrition diet could lead to more serious chondrocyte necrosis in the epiphyseal plate and disturb metaphyseal trabecular bone formation.
Yao
Yun-fen
YF
Orthopedic Department, West China Hospital, Sichuan University, Chengdu, People's Republic of China. bb_yaoyunfeng@126.com
Kang
Peng-de
PD
Li
Xing-bo
XB
Yang
Jing
J
Shen
Bing
B
Zhou
Zong-ke
ZK
Pei
Fu-xing
FX
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D019902:Chondrocytes / Q000187:drug effects / Q000473:pathology; D004032:Diet / Q000009:adverse effects*; D004838:Epiphyses / Q000187:drug effects*; D005260:Female; D006132:Growth Plate / Q000187:drug effects*; D006614:Hindlimb; D008297:Male; D009336:Necrosis / Q000139:chemically induced; D009748:Nutrition Disorders; D051381:Rats; D017208:Rats, Wistar; D013605:T-2 Toxin / Q000633:toxicity*
D013605:T-2 Toxin