Development of a novel PCR method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections.
OBJECTIVE
The objective of this study was to develop a novel polymerase chain reaction (PCR) method to comprehensively analyze salivary bacterial flora.


METHODS
The bacterial flora in the saliva of 10 healthy persons and 11 patients with odontogenic infections were examined using a DNA extraction method with a high level of cell destruction efficiency and a novel universal primer set to amplify approximately 580 bp of the 16S rDNA sequence.


RESULTS
Streptococcus (54.5%), Neisseria (14.7%), Actinomyces (8.4%), Gemella (4.1%), Granulicatella (3.8%), and Prevotella (1.4%) were dominant in a total of 1655 clones examined from the saliva of the healthy subjects. The dominant genera differed among the patients with odontogenic infections (a total of 823 clones) and were entirely different from those of the healthy subjects.


CONCLUSIONS
This novel comprehensive salivary bacterial flora analysis method may be a useful supportive method to identify causative agents of odontogenic infections.
Akiyama
Tomonori
T
Department of Oral and Maxillofacial Surgery, Saga University, Saga, Japan.
Miyamoto
Hiroshi
H
Fukuda
Kazumasa
K
Sano
Naoto
N
Katagiri
Nanako
N
Shobuike
Takeo
T
Kukita
Akiko
A
Yamashita
Yoshio
Y
Taniguchi
Hatsumi
H
Goto
Masaaki
M
D000190:Actinomyces / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001419:Bacteria / Q000145:classification*; D001424:Bacterial Infections / Q000175:diagnosis*; D001431:Bacteriological Techniques; D016866:Bacteroidaceae Infections / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009059:Mouth Diseases / Q000382:microbiology*; D009343:Neisseria / Q000302:isolation & purification; D016870:Neisseriaceae Infections / Q000382:microbiology; D010462:Peptostreptococcus / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D018720:Prevotella / Q000302:isolation & purification; D012463:Saliva / Q000382:microbiology*; D040141:Staphylococcaceae / Q000302:isolation & purification; D013290:Streptococcal Infections / Q000382:microbiology; D013291:Streptococcus / Q000302:isolation & purification; D055815:Young Adult
D004269:DNA, Bacterial; D004275:DNA, Ribosomal