Depth of field multiplexing in microscopy.
We demonstrate "depth of field multiplexing" by a high resolution spatial light modulator (SLM) in a Fourier plane in the imaging path of a standard microscope. This approach provides simultaneous imaging of different focal planes in a sample with only a single camera exposure. The phase mask on the SLM corresponds to a set of superposed multi-focal off-axis Fresnel lenses, which sharply image different focal planes of the object to non-overlapping adjacent sections of the camera chip. Depth of field multiplexing allows to record motion in a three dimensional sample volume in real-time, which is exemplarily demonstrated for cytoplasmic streaming in plant cells and rapidly swimming protozoa.
Maurer
Christian
C
Innsbruck Medical University, Division for Biomedical Physics, Müllerstrasse 44, 6020 Innsbruck, Austria.
Khan
Saranjam
S
Fassl
Stephanie
S
Bernet
Stefan
S
Ritsch-Marte
Monika
M
D003595:Cytoplasmic Streaming; D005055:Euglena / Q000166:cytology*; D035264:Flowers / Q000166:cytology; D008853:Microscopy / Q000379:methods*; D034823:Tradescantia / Q000166:cytology*