title: Purification of the Staphylococcus aureus bacteriophages VDX-10 on methacrylate monoliths.
abstract: Bacteriophages (phages) are known to be useful in many fields from medicine to agriculture, and for a broad range of applications, including phage therapy and phage display. For some applications, especially in medicine, high purity and viability of phages are required. Methacrylate monoliths (Convective Interaction Media [CIM] monolithic columns), designed for purification of bionanoparticles, were applied for the purification of Staphylococcus aureus phages VDX-10 from bacterial lysate. With a single step purification method, more than 99% of host cell DNA and more than 90% of proteins were removed, with 60% recovery of viable phages. Comparable results were obtained when the purification method was scaled-up from a CIM monolithic disk to a larger CIM monolithic column. Additionally, the dynamic binding capacity of a methacrylate monolith column for S. aureus phages VDX-10 was determined.
authors.affiliation: BIA Separations, d.o.o., Teslova 30, SI-1000 Ljubljana, Slovenia. petra.kramberger@monoliths.com
authors.forename: Petra
authors.initials: P
authors.lastname: Kramberger
authors.forename: Richard C
authors.initials: RC
authors.lastname: Honour
authors.forename: Richard E
authors.initials: RE
authors.lastname: Herman
authors.forename: Franci
authors.initials: F
authors.lastname: Smrekar
authors.forename: Matjaz
authors.initials: M
authors.lastname: Peterka
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods; D008689:Methacrylates; D013204:Staphylococcus Phages / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000821:virology*; D014773:Virology / Q000379:methods*
chemical_list: D008689:Methacrylates