title: Imprint cytology in tumor tissue bank quality control: an efficient method to evaluate tumor necrosis and to detect samples without tumor cells.
abstract: Quality assessment of the tissue stored in a tumor biobank is crucial because it is estimated that approximately 10% of the frozen samples are unsuitable for a molecular analysis mainly because of sampling problems in the tissue. We studied the value of imprint cytology (IC) versus frozen section to quantify necrosis and tumor cells in the tissue. The amount of tumor cells and necrosis was assessed by one pathologist on the frozen sections and ICs independently on 100 consecutive tumor samples. It was expressed as a percentage on frozen sections and on a four-level semiquantitative scale for IC (0 to 3+). Overall agreement between the quantity of tumor cells on IC and on frozen section was fair (Kappa = 0.23). Sensitivity and specificity of IC to detect the absence of tumor cell on the frozen section were 57% (4/7) and 98% (91/93), respectively. Overall agreement between necrosis quantification on IC and on frozen section was substantial (Kappa = 0.66).Sensitivity and specificity of IC to detect significant necrosis (defined as more than 30% necrosis) were 100% (3/3) and 98% (95/97), respectively. We show that IC is efficient to semiquantify necrosis in a tumor sample and to detect significant necrosis. IC seems to be less efficient to quantify tumor cells.
authors.affiliation: Service d'Anatomie et Cytologie Pathologiques, AP-HP, Hôpital Saint-Antoine, 184 rue du faubourg St Antoine, 75571 Paris Cedex 12, France.
authors.forename: Pascaline
authors.initials: P
authors.lastname: Boudou-Rouquette
authors.forename: Noura
authors.initials: N
authors.lastname: Touibi
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Boëlle
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Tiret
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Fléjou
authors.forename: Dominique
authors.initials: D
authors.lastname: Wendum
mesh_terms: D005629:Frozen Sections; D006801:Humans; D054802:Molecular Imprinting / Q000379:methods*; D009336:Necrosis; D009369:Neoplasms / Q000473:pathology*; D011237:Predictive Value of Tests; D011786:Quality Control; D012680:Sensitivity and Specificity; D014015:Tissue Banks