title: Simple and conveniently accessible bi-fluorescence-labeled substrates for amylases.
abstract: Synthesis of bi-fluorescence-labeled maltooligosaccharides for amylase assay was accomplished. Preliminary biological evaluation of both bi-fluorescence-labeled maltohexasaccharide and maltose using alpha-amylase was carried out, and the hexaosyl derivative showed unique variation on the basis of fluorescence resonance energy transfer (FRET).
authors.affiliation: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Oka
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Koyama
authors.forename: Ken
authors.initials: K
authors.lastname: Hatano
authors.forename: Daiyo
authors.initials: D
authors.lastname: Terunuma
authors.forename: Koji
authors.initials: K
authors.lastname: Matsuoka
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D000516:alpha-Amylases