title: Slit1 promotes regenerative neurite outgrowth of adult dorsal root ganglion neurons in vitro via binding to the Robo receptor.
abstract: Secreted Slit proteins have previously been shown to signal through Roundabout (Robo) receptors to negatively regulate axon guidance and cell migration. During vertebrate development, Slit proteins have also been shown to stimulate branching and elongation of sensory axons and cortical dendrites. In this study, Slit1/Robo2 mRNA and protein expressions were detected in adult rat dorsal root ganglion (DRG) and in cultured DRG neurons. Treatment of both models with recombinant, soluble Slit1 protein was found to promote neurite outgrowth and elongation. In contrast, treatment with a recombinant human Robo2/Fc chimera inhibited neurite outgrowth and elongation. When adult DRG and cultured DRG neurons were pretreated with soluble recombinant human Robo2/Fc chimera, neurite outgrowth and elongation was not induced. These findings indicate that Slit1/Robo2 signaling may have a role in regulating peripheral nerve regeneration.
authors.affiliation: Department of Anatomy & Neurobiology, Hainan Medical College, Haikou 571101, Hainan, China.
authors.forename: Hai Ying
authors.initials: HY
authors.lastname: Zhang
authors.forename: Lin Feng
authors.initials: LF
authors.lastname: Zheng
authors.forename: Xi Nan
authors.initials: XN
authors.lastname: Yi
authors.forename: Zhi Bin
authors.initials: ZB
authors.lastname: Chen
authors.forename: Zhong Ping
authors.initials: ZP
authors.lastname: He
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Xian Fang
authors.initials: XF
authors.lastname: Zhang
authors.forename: Zhi Jian
authors.initials: ZJ
authors.lastname: Ma
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D020439:Growth Cones / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D055495:Neurogenesis / Q000502:physiology; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D011971:Receptors, Immunologic / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D011984:Sensory Receptor Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000097905:Roundabout Proteins
chemical_list: D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; C516057:Robo2 protein, rat; D000097905:Roundabout Proteins; C492288:Slit1 protein, rat