Preparation and characteristics of protein molecularly imprinted membranes on the surface of multiwalled carbon nanotubes.
A novel protein molecularly imprinted membrane (PMIM) was synthesized on the surface of multiwalled carbon nanotubes (MWNTs) through a surface molecular imprinting technique by using bovine serum albumin (BSA) as the template molecule, acrylamide (AAm) as the functional monomer, N,N'-methylenebisacrylamide (NNMBA) as the cross-linker and ammonium persulphate [(NH(4))(2)S(2)O(8)] as the initiator. The amounts of raw materials were optimized in this paper and the suitable amount is 0.1g of CNTs, 0.02 g of BSA, 0.24 g of AAm, 0.1g of MBA and 0.025 mg of (NH(4))(2)S(2)O(8). The selective recognition ability of PMIM/MWNTs was evaluated using adsorption experiments. Maximum adsorption capacity was 5.53 microg/mg PMIM/MWNTs and a saturation value was achieved at a BSA concentration of 0.2mg/mL. The selectivity adsorption experiments showed that the PMIM/MWNTs also had higher adsorption capacities for BSA than for such molecules, as HSA, HB, pepsin and HRP. The PMIM/MWNTs displayed a 2.6-fold increase in affinity to BSA compared to the nPMIM/MWNTs. The PMIM/MWNTs, on the other hand, did not exhibit any significant change in affinity to other molecules compared to the nPMIM/MWNTs. In the experiment, the characteristics of the PMIM/MWNTs were analyzed using infrared spectroscopy and their configurations were observed using scanning electron microscopy.
Zhang
Maosheng
M
Department of Chemistry and Environmental Science, Fujian Province University Key Laboratory of Analytical Science, Zhangzhou Normal University, Zhangzhou 363000, China.
Huang
Jiarong
J
Yu
Ping
P
Chen
Xi
X
D020106:Acrylamide / Q000737:chemistry; D000178:Acrylamides / Q000737:chemistry; D000327:Adsorption; D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D007700:Kinetics; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013379:Substrate Specificity; D013499:Surface Properties
D000178:Acrylamides; D003432:Cross-Linking Reagents; D008567:Membranes, Artificial; D037742:Nanotubes, Carbon; D020106:Acrylamide; C031276:ammonium peroxydisulfate; D012710:Serum Albumin, Bovine; C021221:N,N'-methylenebisacrylamide; D000645:Ammonium Sulfate