Novel TaqMan real-time polymerase chain reaction assay for verifying the authenticity of meat and commercial meat products from game birds.
Species-specific real-time polymerase chain reaction (PCR) assays using TaqMan probes have been developed for verifying the labeling of meat and commercial meat products from game birds, including quail, pheasant, partridge, guinea fowl, pigeon, Eurasian woodcock and song thrush. The method combines the use of species-specific primers and TaqMan probes that amplify small fragments (amplicons <150 base pairs) of the mitochondrial 12S rRNA gene, and an endogenous control primer pair that amplifies a 141-bp fragment of the nuclear 18S rRNA gene from eukaryotic DNA. Analysis of experimental raw and heat-treated binary mixtures as well as of commercial meat products from the target species demonstrated the suitability of the assay for the detection of the target DNAs.
Rojas
María
M
Departamento de Nutricion, Bromatologia y Tecnologia de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
González
Isabel
I
Pavón
Miguel Angel
MA
Pegels
Nicolette
N
Lago
Adriana
A
Hernández
Pablo E
PE
García
Teresa
T
Martín
Rosario
R
D000818:Animals; D001717:Birds / Q000235:genetics; D002417:Cattle; D004247:DNA / Q000235:genetics; D017931:DNA Primers; D003670:Deer; D005515:Food Labeling / Q000331:legislation & jurisprudence / Q000592:standards; D008460:Meat / Q000592:standards*; D008461:Meat Products / Q000592:standards*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011784:Quail / Q000235:genetics; D012680:Sensitivity and Specificity; D012756:Sheep; D020308:Songbirds / Q000235:genetics; D013552:Swine
D017931:DNA Primers; D004247:DNA