Bone induction by biomimetic PLGA-(PEG-ASP)n copolymer loaded with a novel synthetic BMP-2-related peptide in vitro and in vivo.
BMP-2 is one of the most important growth factors of bone regeneration. Polylactide-co-glycolic acid (PLGA), which is used as a biodegradable scaffold for delivering therapeutic agents, has been intensively investigated. In previous studies, we synthesized a novel BMP-2-related peptide (designated P24) and found that it could enhance the osteoblastic differentiation of bone marrow stromal cells (BMSCs). The objective of this study was to construct a biomimetic composite by incorporating P24 into a modified PLGA-(PEG-ASP)n copolymer to promote bone formation. In vitro, our results demonstrated that PLGA-(PEG-ASP)n scaffolds were shown to be an efficient system for sustained release of P24. Significantly more BMSCs attached to the P24/PLGA-(PEG-ASP)n and PLGA-(PEG-ASP)n membranes than to PLGA, and the cells in the two groups subsequently proliferated more vigorously than those in the PLGA group. The expression of osteogenic markers in P24/PLGA-(PEG-ASP)n group was stronger than that in the PLGA-(PEG-ASP)n and PLGA groups. Radiographic and histological examination, Western blotting and RT-PCR showed that P24/PLGA-(PEG-ASP)n scaffold could induce more effective ectopic bone formation in vivo, as compared with PLGA-(PEG-ASP)n or gelatin sponge alone. It is concluded that the PLGA-(PEG-ASP)n copolymer is a good P24 carrier and can serve as a good scaffold for controlled release of P24. This novel P24/PLGA-(PEG-ASP)n composite promises to be an excellent biomaterial for inducing bone regeneration.
Lin
Zhen-Yu
ZY
Department of Orthopedics, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Duan
Zhi-Xia
ZX
Guo
Xiao-Dong
XD
Li
Jing-Feng
JF
Lu
Hong-Wei
HW
Zheng
Qi-Xin
QX
Quan
Da-Ping
DP
Yang
Shu-Hua
SH
D000818:Animals; D001215:Asparaginase; D001672:Biocompatible Materials / Q000378:metabolism; D032701:Biomimetics; D055396:Bone Morphogenetic Protein 2; D001861:Bone Regeneration / Q000187:drug effects*; D001842:Bone and Bones / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D017886:Durapatite / Q000378:metabolism; D006016:Glycolates; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D010455:Peptides / Q000378:metabolism; D011091:Polyesters; D011092:Polyethylene Glycols; D011108:Polymers / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D006016:Glycolates; D010455:Peptides; D011091:Polyesters; D011108:Polymers; C031149:glycolic acid; D011092:Polyethylene Glycols; C033616:poly(lactide); C042705:pegaspargase; D017886:Durapatite; D001215:Asparaginase