title: Crystal structure of the transcriptional activator HlyU from Vibrio vulnificus CMCP6.
abstract: HlyU is a transcription factor of the ArsR/SmtB family and activates the expression of the pathogenic Vibrio vulnificus RTX toxin. In contrast to the other metal-responding ArsR/SmtB proteins, HlyU does not sense metal ions. To provide its structural information, we elucidated the crystal structure of HlyU from V. vulnificus CMCP6 (HlyU_Vv). The monomeric HlyU_Vv architecture of five alpha-helices and two beta-strands, some of which constitute a typical DNA-binding winged helix-turn-helix (wHTH) motif, is very similar to that of other transcription regulators. Nonetheless, the homo-dimeric HlyU_Vv structure shows several different, three-dimensional features in the spatial position and the detailed dimeric interaction, which were not observed in the modeling study based on the same protein family and sequence similarity.
authors.affiliation: Department of Chemistry, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Nishi
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Suk-Youl
authors.initials: SY
authors.lastname: Park
authors.forename: Soo Jang
authors.initials: SJ
authors.lastname: Bae
authors.forename: Shee Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Paul D
authors.initials: PD
authors.lastname: Adams
authors.forename: Joon Haeng
authors.initials: JH
authors.lastname: Rhee
authors.forename: Jeong-Sun
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004269:DNA, Bacterial / Q000378:metabolism; D006459:Hemolysin Factors / Q000737:chemistry* / Q000378:metabolism; D019216:Metals, Heavy / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D055503:Protein Multimerization; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism; D041261:Vibrio vulnificus / Q000235:genetics / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D019216:Metals, Heavy; D015534:Trans-Activators