Cu,Zn-superoxide dismutase is required for cell wall structure and for tolerance to cell wall-perturbing agents in Saccharomyces cerevisiae.
Here we report that deletion of SOD1, the Cu,Zn-superoxide dismutase in Saccharomyces cerevisiae is sensitive to cell wall-perturbing agents, such as Calcofluor white and Congo red. The sensitivity was restored by retransformation with wild type SOD1 or the addition of N-acetylcysteine or reduced glutathione to the medium. Additionally, the accumulation of reactive oxygen species was observed in sod1Delta mutant in the presence of Calcofluor white or Congo red. Cell wall analysis indicated an increase of cell wall chitin and cell wall thickness in sod1Delta mutant compared to wild type. These results indicate a novel direct connection between antioxidative functions and cell wall homeostasis.
Liu
Xiangyong
X
Department of Cell Biology, Binzhou Medical University, Yantai, China. xyliu@bzmc.edu.cn
Zhang
Xiaohua
X
Zhang
Zhaojie
Z
D000935:Antifungal Agents / Q000494:pharmacology; D001557:Benzenesulfonates / Q000494:pharmacology; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D002686:Chitin / Q000032:analysis / Q000378:metabolism; D003224:Congo Red / Q000494:pharmacology; D025141:Drug Resistance, Fungal / Q000235:genetics*; D017353:Gene Deletion; D008826:Microbial Sensitivity Tests; D030781:Organisms, Genetically Modified; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure*; D013482:Superoxide Dismutase / Q000235:genetics / Q000502:physiology*
D000935:Antifungal Agents; D001557:Benzenesulfonates; D017382:Reactive Oxygen Species; D002686:Chitin; D003224:Congo Red; C007061:C.I. Fluorescent Brightening Agent 28; D013482:Superoxide Dismutase