title: Exogenous allogenic fragmented double-stranded DNA is internalized into human dendritic cells and enhances their allostimulatory activity.
abstract: Exogenous allogenic DNA as nucleosome-free fragments reaches main cellular compartments (cytoplasm, nucleus) of human dendritic cells and deposits in the nuclear interchromosomal space without visibly changing in linear size. The presence of such allogenic fragmented DNA in medium in which human dendritic cells are cultured produces an enhancement of their allostimulatory activity. This enhancement is comparable to that produced by the standard maturation stimulus lipopolysaccharide Escherichia coli.
authors.affiliation: Novosibirsk State University, Novosibirsk, Russia.
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Alyamkina
authors.forename: Evgenia V
authors.initials: EV
authors.lastname: Dolgova
authors.forename: Anastasia S
authors.initials: AS
authors.lastname: Likhacheva
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Rogachev
authors.forename: Tamara E
authors.initials: TE
authors.lastname: Sebeleva
authors.forename: Valeriy P
authors.initials: VP
authors.lastname: Nikolin
authors.forename: Nelly A
authors.initials: NA
authors.lastname: Popova
authors.forename: Elena V
authors.initials: EV
authors.lastname: Kiseleva
authors.forename: Konstantin E
authors.initials: KE
authors.lastname: Orishchenko
authors.forename: Ludmila V
authors.initials: LV
authors.lastname: Sakhno
authors.forename: Evgeniy L
authors.initials: EL
authors.lastname: Gel'fgat
authors.forename: Alexandr A
authors.initials: AA
authors.lastname: Ostanin
authors.forename: Elena R
authors.initials: ER
authors.lastname: Chernykh
authors.forename: Stanislav N
authors.initials: SN
authors.lastname: Zagrebelniy
authors.forename: Sergey S
authors.initials: SS
authors.lastname: Bogachev
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Shurdov
mesh_terms: D000818:Animals; D004247:DNA / Q000276:immunology* / Q000648:ultrastructure; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004705:Endocytosis / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D051379:Mice
chemical_list: D004247:DNA