Characterization of bacteriocin produced by Streptococcus bovis J2 40-2 isolated from traditional fermented milk 'Dahi'.
A bacteriocin-producing strain Streptococcus bovis J2 40-2 was isolated from traditional fermented milk 'Dahi' in Bangladesh. Despite its narrow antimicrobial spectrum, it showed strong antimicrobial activity against extremely challenging and problematic organisms in foods, such as Listeria monocytogenes. Bacteriocin was sensitive to several proteolytic enzymes and showed antimicrobial activity over a wide pH range of 2.0-10.0. It was stable when heated to 110 degrees C for 20 min, but lost 25% of its activity when heated to 121 degrees C for 15 or 20 min. Optimum bacteriocin production (5600 AU/mL) was achieved when the strain was cultured at 37 degrees C for 24 h in MRS medium rather than in TYLG, GM17, or skim milk medium. Bacteriocin was partially purified by ammonium sulfate precipitation (80% saturation), dialysis (cut-off MW: 3500) and gel filtration chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that bacteriocin had a molecular weight of approximately 4.5 kDa.
Rashid
Md Harun-ur
MH
Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
Togo
Kaname
K
Ueda
Minoru
M
Miyamoto
Taku
T
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D001430:Bacteriocins / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry*; D001459:Bangladesh; D002417:Cattle; D046508:Culture Techniques; D043302:Cultured Milk Products / Q000737:chemistry* / Q000382:microbiology; D005260:Female; D005516:Food Microbiology; D005519:Food Preservation; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000187:drug effects; D010447:Peptide Hydrolases; D016985:Streptococcus bovis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010447:Peptide Hydrolases