Rapid Cu-free click chemistry with readily synthesized biarylazacyclooctynones.
Bioorthogonal chemical reactions, those that do not interact or interfere with biology, have allowed for exploration of numerous biological processes that were previously difficult to study. The reaction of azides with strained alkynes, such as cyclooctynes, readily forms a triazole product without the need for a toxic catalyst. Here we describe a biarylazacyclooctynone (BARAC) that has exceptional reaction kinetics and whose synthesis is designed to be both modular and scalable. We employed BARAC for live cell fluorescence imaging of azide-labeled glycans. The high signal-to-background ratio obtained using nanomolar concentrations of BARAC obviated the need for washing steps. Thus, BARAC is a promising reagent for in vivo imaging.
Jewett
John C
JC
Department of Chemistry and Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA.
Sletten
Ellen M
EM
Bertozzi
Carolyn R
CR
D000480:Alkynes / Q000138:chemical synthesis* / Q000737:chemistry*; D000818:Animals; D001386:Azides / Q000737:chemistry; D016466:CHO Cells; D002470:Cell Survival; D003300:Copper / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D007700:Kinetics; D057054:Molecular Imaging; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism
D000480:Alkynes; D001386:Azides; D011134:Polysaccharides; D003300:Copper