title: Nosokomycins, new antibiotics discovered in an in vivo-mimic infection model using silkworm larvae. I: Fermentation, isolation and biological properties.
abstract: The in vivo-mimic assay system using silkworm larvae was used as a screening tool to discover antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). Microbial culture broths were screened in this in vivo-mimic assay system and a culture broth of Streptomyces sp. K04-0144 was selected. New antibiotics, designated nosokomycins A-D, were isolated from the culture broth by HP-20 and ODS column chromatography and HPLC. Nosokomycins inhibited the growth of MRSA with MIC values of 0.125 microg ml(-1) using the liquid microdilution method. Furthermore, MRSA-infected silkworms survived when nosokomycin A or B was injected at a dose of 50 microg per larva.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Uchida
authors.forename: Masato
authors.initials: M
authors.lastname: Iwatsuki
authors.forename: Yong-Pil
authors.initials: YP
authors.lastname: Kim
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohte
authors.forename: Satoshi
authors.initials: S
authors.lastname: Omura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tomoda
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology*; D012831:Bombyx; D002240:Carbohydrate Sequence; D005285:Fermentation; D007814:Larva; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D013203:Staphylococcal Infections / Q000188:drug therapy*; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; D012717:Sesquiterpenes