title: The transcriptional repressor Kaiso localizes at the mitotic spindle and is a constituent of the pericentriolar material.
abstract: Kaiso is a BTB/POZ zinc finger protein known as a transcriptional repressor. It was originally identified through its in vitro association with the Armadillo protein p120ctn. Subcellular localization of Kaiso in cell lines and in normal and cancerous human tissues revealed that its expression is not restricted to the nucleus. In the present study we monitored Kaiso's subcellular localization during the cell cycle and found the following: (1) during interphase, Kaiso is located not only in the nucleus, but also on microtubular structures, including the centrosome; (2) at metaphase, it is present at the centrosomes and on the spindle microtubules; (3) during telophase, it accumulates at the midbody. We found that Kaiso is a genuine PCM component that belongs to a pericentrin molecular complex. We analyzed the functions of different domains of Kaiso by visualizing the subcellular distribution of GFP-tagged Kaiso fragments throughout the cell cycle. Our results indicate that two domains are responsible for targeting Kaiso to the centrosomes and microtubules. The first domain, designated SA1 for spindle-associated domain 1, is located in the center of the Kaiso protein and localizes at the spindle microtubules and centrosomes; the second domain, SA2, is an evolutionarily conserved domain situated just before the zinc finger domain and might be responsible for localizing Kaiso towards the centrosomal region. Constructs containing both SA domains and Kaiso's aminoterminal BTB/POZ domain triggered the formation of abnormal centrosomes. We also observed that overexpression of longer or full-length Kaiso constructs led to mitotic cell arrest and frequent cell death. Knockdown of Kaiso accelerated cell proliferation. Our data reveal a new target for Kaiso at the centrosomes and spindle microtubules during mitosis. They also strongly imply that Kaiso's function as a transcriptional regulator might be linked to the control of the cell cycle and to cell proliferation in cancer.
authors.affiliation: Department for Molecular Biomedical Research, VIB, Ghent, Belgium.
authors.forename: Adelheid
authors.initials: A
authors.lastname: Soubry
authors.forename: Katrien
authors.initials: K
authors.lastname: Staes
authors.forename: Eef
authors.initials: E
authors.lastname: Parthoens
authors.forename: Sam
authors.initials: S
authors.lastname: Noppen
authors.forename: Christophe
authors.initials: C
authors.lastname: Stove
authors.forename: Pieter
authors.initials: P
authors.lastname: Bogaert
authors.forename: Jolanda
authors.initials: J
authors.lastname: van Hengel
authors.forename: Frans
authors.initials: F
authors.lastname: van Roy
mesh_terms: D015153:Blotting, Western; D002453:Cell Cycle; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002502:Centrioles / Q000378:metabolism; D018385:Centrosome / Q000378:metabolism*; D048749:Cytokinesis; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008938:Mitosis; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C492254:ZBTB33 protein, human; D049452:Green Fluorescent Proteins