Revealing a signaling role of phytosphingosine-1-phosphate in yeast.
Sphingolipids including sphingosine-1-phosphate and ceramide participate in numerous cell programs through signaling mechanisms. This class of lipids has important functions in stress responses; however, determining which sphingolipid mediates specific events has remained encumbered by the numerous metabolic interconnections of sphingolipids, such that modulating a specific lipid of interest through manipulating metabolic enzymes causes 'ripple effects', which change levels of many other lipids. Here, we develop a method of integrative analysis for genomic, transcriptomic, and lipidomic data to address this previously intractable problem. This method revealed a specific signaling role for phytosphingosine-1-phosphate, a lipid with no previously defined specific function in yeast, in regulating genes required for mitochondrial respiration through the HAP complex transcription factor. This approach could be applied to extract meaningful biological information from a similar experimental design that produces multiple sets of high-throughput data.
Cowart
L Ashley
LA
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA.
Shotwell
Matthew
M
Worley
Mitchell L
ML
Richards
Adam J
AJ
Montefusco
David J
DJ
Hannun
Yusuf A
YA
Lu
Xinghua
X
D001499:Bayes Theorem; D016000:Cluster Analysis; D050356:Lipid Metabolism; D053858:Metabolic Networks and Pathways; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013107:Sphingolipids / Q000378:metabolism; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism; D049490:Systems Biology / Q000379:methods; D014157:Transcription Factors
D029701:Saccharomyces cerevisiae Proteins; D013107:Sphingolipids; D014157:Transcription Factors; C117422:phytosphingosine-1-phosphate; D013110:Sphingosine