Enhanced anticancer activity of multi-walled carbon nanotube-methotrexate conjugates using cleavable linkers.
Methotrexate was tethered to multi-walled carbon nanotubes through different cleavable linkers exploiting the ammonium functionalities introduced by 1,3-dipolar cycloaddition reaction of azomethine ylides to the nanotubes. The new nanobio-hybrid conjugates were internalized into human breast cancer cells and it was shown that the cytotoxic activity was strongly dependent on the presence and type of linker.
Samorì
Cristian
C
CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunologie et Chimie Thérapeutiques, 67000 Strasbourg, France.
Ali-Boucetta
Hanene
H
Sainz
Raquel
R
Guo
Chang
C
Toma
Francesca Maria
FM
Fabbro
Chiara
C
da Ros
Tatiana
T
Prato
Maurizio
M
Kostarelos
Kostas
K
Bianco
Alberto
A
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D001391:Azo Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006801:Humans; D008727:Methotrexate / Q000737:chemistry* / Q000633:toxicity; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D013882:Thiosemicarbazones / Q000737:chemistry
D000970:Antineoplastic Agents; D001391:Azo Compounds; D003432:Cross-Linking Reagents; D037742:Nanotubes, Carbon; D013882:Thiosemicarbazones; C512188:azomethine; D008727:Methotrexate