title: Modeling and optimization of hairy root growth in fed-batch process.
abstract: This article proposes a feeding strategy based on a kinetic model to enhance hairy roots growth. A new approach for modeling hairy root growth is used, considering that there is no nutrient limitation thanks to an appropriate feeding, and the intracellular pools are supposed to be always saturated. Thus, the model describes the specific growth rate from extracellular concentration of the major nutrients and nutrient uptakes depend on biomass growth. An optimized feeding strategy was determined thanks to the model to maintain the major nutrient levels at their optimum assuming optimal initial concentrations. The optimal feed rate is computed in open loop using kinetic model prediction or in closed loop using conductivity measurements to estimate biomass growth. Datura innoxia was chosen as the model culture system. Shake flask cultures were used to calibrate the model. Finally, cultures in bioreactor were performed to validate the model and the control laws.
authors.affiliation: Transformations Intégrées de Matière Renouvelable, Université de Technologie de Compiègne, BP 649, Compiègne 60206, France. francis.mairet@inria.fr
authors.forename: Francis
authors.initials: F
authors.lastname: Mairet
authors.forename: Pierre
authors.initials: P
authors.lastname: Villon
authors.forename: Michèle
authors.initials: M
authors.lastname: Boitel-Conti
authors.forename: Khalil
authors.initials: K
authors.lastname: Shakourzadeh
mesh_terms: D000465:Algorithms; D018533:Biomass; D019149:Bioreactors; D003198:Computer Simulation; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D032302:Datura / Q000254:growth & development* / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism; D012044:Regression Analysis; D015203:Reproducibility of Results; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media