title: Development of a device for chemiluminescence determination of superoxide generated inside a dialysis hollow-fiber membrane.
abstract: Reactive oxygen species (ROS) generated during hemodialysis treatment cause dialysis complications because of the high reactivity of ROS. To prevent dialysis complications caused by oxidative stress, it is important to evaluate the generation and dismutation of ROS during hemodialysis treatment. In this study, our aim was to develop a device to determine superoxide (O(2)(-)) generated inside a dialysis hollow fiber, and also to examine whether this device could detect O(2)(-) separated from plasma using hollow fibers. Experimental apparatus was set up so that hypoxanthine (HX) solution flowed inside the hollow fibers and 2-methyl-6-p-methoxyphenylethynyl-imidazopyrazinone (MPEC) solution flowed outside the hollow fibers. Then, xanthine oxidase (XOD) solution was added to the HX solution to generate O(2)(-), and chemiluminescence resulting from the reaction of O(2)(-) with MPEC was measured with an optical fiber. Chemiluminescence intensity was measured at different HX concentrations, and the peak area of relative luminescence intensity yielded a first-order correlation with the HX concentration. Based on the relationship between HX and O(2)(-) concentrations determined by the cytochrome c reduction method, the relative luminescence intensity measured by this device was linearly dependent on the O(2)(-) concentration inside the hollow fibers. After modifications were made to the device, XOD solution injection into plasma including HX resulted in an increase in the relative luminescence intensity. We concluded that this novel device based on chemiluminescence is capable of determining aqueous O(2)(-) generated inside a hollow fiber and also of detecting O(2)(-) in plasma.
authors.affiliation: Department of Chemical Engineering, Waseda University, Shinjuku-ku, Tokyo, Japan.
authors.forename: Takehito
authors.initials: T
authors.lastname: Ogawa
authors.forename: Sumire
authors.initials: S
authors.lastname: Koga
authors.forename: Taiji
authors.initials: T
authors.lastname: Yakushiji
authors.forename: Masato
authors.initials: M
authors.lastname: Matsuda
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sakata
authors.forename: Makoto
authors.initials: M
authors.lastname: Fukuda
authors.forename: Takehiro
authors.initials: T
authors.lastname: Miyasaka
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Sakai
mesh_terms: D049449:Luminescence; D008567:Membranes, Artificial; D018384:Oxidative Stress; D006435:Renal Dialysis / Q000295:instrumentation*; D013481:Superoxides / Q000032:analysis*; D014969:Xanthine Oxidase / Q000032:analysis
chemical_list: D008567:Membranes, Artificial; D013481:Superoxides; D014969:Xanthine Oxidase