title: Efficient treatment of garbage slurry in methanogenic bioreactor packed by fibrous sponge with high porosity.
abstract: Adding a supporting material to a methanogenic bioreactor treating garbage slurry can improve efficiency of methane production. However, little is known on how characteristics (e.g., porosity and hydrophobicity) of the supporting material affect the bioreactor degrading garbage slurry. We describe the reactor performances and microbial communities in bioreactors containing hydrophilic or hydrophobic sheets, or fibrous hydrophilic or hydrophobic sponges. The porosity affected the efficiency of methane production and solid waste removal more than the hydrophilic or hydrophobic nature of the supporting material. When the terminal restriction fragment length polymorphism technique was used at a lower organic loading rate (OLR), microbial diversities in the suspended fraction were retained on the hydrophobic, but not the hydrophilic, sheets. Moreover, real-time quantitative polymerase chain reaction (PCR) performed at a higher OLR revealed that the excellent performance of reactors containing fibrous sponges with high porosity (98%) was supported by a clear increase in the numbers of methanogens on these sponges, resulting in larger total numbers of methanogens in the reactors. In addition, the bacterial communities in fractions retained on both the hydrophobic and hydrophilic fibrous sponges differed from those in the suspended fraction, thus increasing bacterial diversity in the reactor. Thus, higher porosity of the supporting material improves the bioreactor performance by increasing the amount of methanogens and bacterial diversity; surface hydrophobicity contributes to maintaining the suspended microbial community.
authors.affiliation: Biotechnology Sector, Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry, 1646 Abiko, Abiko-shi, Chiba-ken, 270-1194, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Sasaki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sasaki
authors.forename: Masahiko
authors.initials: M
authors.lastname: Morita
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Hirano
authors.forename: Norio
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Naoya
authors.initials: N
authors.lastname: Ohmura
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Igarashi
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon; D019605:Euryarchaeota / Q000302:isolation & purification / Q000378:metabolism*; D005735:Garbage; D005898:Glass; D008697:Methane / Q000032:analysis; D020959:Polyethylene; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016062:Porosity
chemical_list: D002244:Carbon; D020959:Polyethylene; D008697:Methane