The toxicity of aconitine, emodin on ICC cell and the antagonist effect of the compatibility.
The study was to investigate the effect and action mechanism of aconitine and emodin on the function of the interstitial cells of Cajal (ICC) cultured in vitro. ICC cells were treated with aconitine (0.05-8%) and emodin (0.001-2%) in single or combined synchronous experiments and the effect of emodin on aconitine was evaluated using cell viability as end-point. Both the two compounds had toxicity on ICC cell. The cell membrane integrity impairment caused by the exposure lead to the efflux of intracellular ionic ([Na+], [Ca2+] and [K+]) and the deactivation of the Na+-K+-ATPase. The ionic disturbance caused the interruption of the cellular breathing chain and resulted in anaerobic metabolism increase and the glycogen massive decomposition, at last the energy metabolism in the cells was obstructed. But the antagonist effect existed when the two compounds were exposed to ICC cells together. The compatibility (aonitine:emodin as 2:1), can significantly reversed the toxicity of aconition. In addition, synergistic effects were never observed in the range of concentrations considered. Although emodin can defer the aconition's toxicity on ICC cell, the impairment can't be totally inhibited by the compatibility with time went on. The results of our work represent a starting point to generate novel information on the interactions between aconitine and emodin in vitro, as well as a new relevant experimental approach useful to investigate the Herb compatibility with aconite and rhubarb and reference for the clinic.
Peng
Cheng
C
Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China. pengchengchengdu@126.com
Wang
Lan
L
Wang
Yan-Hong
YH
Li
Yun-Xia
YX
Pan
Yuan
Y
D000157:Aconitine / Q000008:administration & dosage / Q000633:toxicity*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000633:toxicity; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004642:Emodin / Q000008:administration & dosage / Q000633:toxicity*; D004734:Energy Metabolism / Q000187:drug effects; D056885:Interstitial Cells of Cajal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D011188:Potassium / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000008:administration & dosage / Q000633:toxicity; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D000276:Adjuvants, Immunologic; D047428:Protein Kinase Inhibitors; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D004642:Emodin; D011188:Potassium; D002118:Calcium; D000157:Aconitine