title: PIM-1 gene RNA interference induces growth inhibition and apoptosis of prostate cancer cells and suppresses tumor progression in vivo.
abstract: BACKGROUND
  The goal of this study was to investigate the roles of PIM-1 in prostate cancer (CaP) cell proliferation and apoptosis, and to assess the potential of PIM-1 as a target for CaP therapy.
  
  
  METHODS
  Using RNAi technology, we knocked down the expression of PIM-1 in PC-3 cell. After siRNA transfection, cell morphology, cell proliferation, cell cycle, and apoptosis rate were analyzed. PIM-1 siRNA with Lipofectamine were injected into xenograft models to evaluate its therapeutic effect.
  
  
  RESULTS
  PIM-1 siRNA significantly inhibited PIM-1 expression. In vitro, silencing of the PIM-1 gene resulted in irregular cell morphology, decreased cell proliferation, inhibition of cell-cycle progression, and induction of apoptosis. Compared with control groups, intratumoral injection of PIM-1 siRNA with Lipofectamine in nude mice dramatically suppressed PC-3 tumor progression.
  
  
  CONCLUSIONS
  PIM-1 could play important roles in the progression of CaP and may be an interesting target for CaP therapy.
authors.affiliation: Tianjin Institute of Urology, Second Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Zhang
authors.forename: Kejia
authors.initials: K
authors.lastname: Ding
authors.forename: Kuo
authors.initials: K
authors.lastname: Yang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology*; D045744:Cell Line, Tumor; D020868:Gene Silencing; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D034741:RNA, Small Interfering