A flow cytometric assay to quantify in vivo bacterial uptake by alveolar macrophages.
Our laboratory has developed a flow cytometric assay to quantify alveolar macrophage (Mcapital EF, Cyrillic) phagocytosis of bacteria within a live animal. Mcapital EF, Cyrillics collected by bronchoalveolar lavage from rats infected transtracheally with Syto 9-labeled bacteria are fluorescently labeled for identification and analyzed by flow cytometry to quantify their bacterial uptake.
Pitz
Adam M
AM
Department of Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA.
Perry
Greg A
GA
Jensen-Smith
Heather C
HC
Gentry-Nielsen
Martha J
MJ
D000818:Animals; D001419:Bacteria / Q000276:immunology*; D018893:Bronchoalveolar Lavage; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000494:pharmacology; D008168:Lung / Q000276:immunology; D016676:Macrophages, Alveolar / Q000382:microbiology*; D008297:Male; D009930:Organic Chemicals / Q000494:pharmacology; D010587:Phagocytosis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D009930:Organic Chemicals; C103389:SYTO 9