The effect of calcipotriol on the expression of human beta defensin-2 and LL-37 in cultured human keratinocytes.
BACKGROUND
Vitamin D has been reported to regulate innate immunity by controlling the expression of antimicrobial peptides (AMPs).


OBJECTIVE
We investigated the effect of calcipotriol on the expression of AMPs in human cultured keratinocytes.


METHODS
Keratinocytes were treated with lipopolysaccharide (LPS), TNF-alpha, Calcipotriol and irradiated with UVB, cultured, and harvested. To assess the expression of human beta defensin-2 and LL-37 in the control group, not exposed to any stimulants, the experimental group was treated with LPS, TNF-alpha, or UVB, and another group was treated again with calcipotriol; reverse transcriptase-polymerase chain reaction, Western blotting, and immunohistochemical staining were performed.


RESULTS
In the experimental group treated with LPS, UVB irradiation, and TNF-alpha, the expression of beta-defensin and LL-37 was increased more than in the control group and then decreased in the experimental group treated with calcipotriol.


CONCLUSIONS
Calcipotriol suppressed HBD-2 and LL-37, which were stimulated by UVB, LPS, and TNF-alpha.
Kim
Beom Joon
BJ
Department of Dermatology, Chung-Ang Medical Research Center, College of Medicine, Chung-Ang University, Seoul 156-755, South Korea.
Rho
Yong Kwan
YK
Lee
Hye In
HI
Jeong
Mi Sook
MS
Li
Kapsok
K
Seo
Seong Jun
SJ
Kim
Myeung Nam
MN
Hong
Chang Kwun
CK
D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014466:Ultraviolet Rays; D023083:beta-Defensins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D054804:Cathelicidins
D023181:Antimicrobial Cationic Peptides; C106641:DEFB4A protein, human; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D023083:beta-Defensins; C055085:calcipotriene; D002117:Calcitriol; D054804:Cathelicidins