Human papillomavirus genotyping using archival vulval dysplastic or neoplastic biopsy tissues: comparison between the INNO-LiPA and linear array assays.
The Roche Linear Array (LA) and Innogenetics INNO-LiPA human papillomavirus (HPV) genotyping assays were compared for paraffin-embedded vulval tissues. The LA detected amplifiable DNA in 28 (57%) out of 49 biopsy specimens, 20 (40%) being HPV genotyped, compared to 49 (100%) and 41 (83%), respectively, detected by the INNO-LiPA. The INNO-LiPA provides greater sensitivity for HPV genotyping in archival tissue.
Tan
Sarah E
SE
Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia. s.tan39@pgrad.unimelb.edu.au
Garland
Suzanne M
SM
Rumbold
Alice R
AR
Tabrizi
Sepehr N
SN
D001706:Biopsy; D005260:Female; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000821:virology*; D016612:Paraffin Embedding; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*; D014846:Vulvar Neoplasms / Q000821:virology*