Upregulation of L-type Ca(v)1 channels in the development of psychological dependence.
Although L-type voltage-dependent Ca2+ channels regulate activity-dependent processes including synaptic plasticity and synapse formation, there are few data on the changes of Ca(v)1 channel expression in psychological dependence. This study investigated the role of L-type Ca(v)1 channel expression in the brain of mouse that was psychologically dependent on methamphetamine (2 mg/kg, subcutaneous injection [s.c.]), cocaine (10 mg/kg, s.c.), and morphine (5 mg/kg, s.c.) with the conditioned place preference paradigm. Intracerebroventricular administration of nifedipine (3, 10, and 30 nmol/mouse) dose-dependently reduced the development of methamphetamine-, cocaine-, and morphine-induced rewarding effect. Under such conditions, protein levels of both Ca(v)1.2 and Ca(v)1.3 in the frontal cortex and the limbic forebrain were significantly increased on methamphetamine-, cocaine-, and morphine-induced psychologically dependent mice. These findings suggest that the upregulation of Ca(v)1.2 and Ca(v)1.3 participated in the development of psychological dependence.
Shibasaki
Masahiro
M
Department of Pharmacology, Kawasaki Medical School, Kurashiki 701-0192, Japan.
Kurokawa
Kazuhiro
K
Ohkuma
Seitaro
S
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism* / Q000503:physiopathology; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000187:drug effects* / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D003042:Cocaine / Q000494:pharmacology; D004195:Disease Models, Animal; D005625:Frontal Lobe / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D008032:Limbic System / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology; D051379:Mice; D009020:Morphine / Q000494:pharmacology; D009543:Nifedipine / Q000494:pharmacology; D019966:Substance-Related Disorders / Q000188:drug therapy / Q000378:metabolism* / Q000503:physiopathology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
C514350:CACNA1C protein, mouse; C485995:Cacna1d protein, mouse; D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D008694:Methamphetamine; D009020:Morphine; D003042:Cocaine; D009543:Nifedipine