3D collagen scaffolds coated with multiwalled carbon nanotubes: initial cell attachment to internal surface.
The cell adhesion in a multiwalled carbon nanotube-coated collagen sponge (MWCNT-coated sponge) was investigated. Immediately after seeding, the cells adhered to the inner surface of the MWCNT-coated sponge and a significantly larger number of cells were observed there than for a pure collagen sponge used as control. On the MWCNT-coated sponge, the cells appeared favorable adhesion and spread in the early stages in the center part of the sponge which cells rarely attached without MWCNT-coating. It was suggested that the physical structure of MWCNTs was effective for initial adhesion of cells from the result of serum-free culture. MWCNT-coating makes the material a suitable three-dimensional scaffold for cell culturing, as opposed to other scaffold systems where such an effect is not seen.
Hirata
Eri
E
Department of Oral Functional Prosthodontics, Division of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Kita-ku, Sapporo, Japan. erieri@den.hokudai.ac.jp
Uo
Motohiro
M
Nodasaka
Yoshinobu
Y
Takita
Hiroko
H
Ushijima
Natsumi
N
Akasaka
Tsukasa
T
Watari
Fumio
F
Yokoyama
Atsuro
A
D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible; D003094:Collagen; D006801:Humans; D037742:Nanotubes, Carbon; D054457:Tissue Scaffolds
D020099:Coated Materials, Biocompatible; D037742:Nanotubes, Carbon; D003094:Collagen