title: Study on the effect of T-2 toxin combined with low nutrition diet on rat epiphyseal plate growth and development.
abstract: The purpose of this study was to observe early lesions of rat epiphyseal plates and metaphysis caused by T-2 toxin and T-2 toxin combined with a low nutrition diet to determine possible pathogenic factors of Kashin-Beck disease (KBD). Ninety Wistar rats were divided into three groups. Group A was fed with a normal diet as control; group B was fed with a normal diet and T-2 toxin; and group C was fed with a low nutrition diet and T-2 toxin. The left knee specimens were collected, fixed in formaldehyde solution, stained by hematoxylin and eosin and Masson. After two weeks, the epiphyseal plate showed necrosis of chondrocytes in groups B and C. After four weeks, more obvious chondrocyte necrosis appeared. The positive rate of Lamellar necrosis in group C was significantly higher than that in groups B and A (P < 0.01). Metaphyseal trabecular bone showed sparse disorder and disruption in group C. T-2 toxin combined with a low nutrition diet could lead to more serious chondrocyte necrosis in the epiphyseal plate and disturb metaphyseal trabecular bone formation.
authors.affiliation: Orthopedic Department, West China Hospital, Sichuan University, Chengdu, People's Republic of China. bb_yaoyunfeng@126.com
authors.forename: Yun-fen
authors.initials: YF
authors.lastname: Yao
authors.forename: Peng-de
authors.initials: PD
authors.lastname: Kang
authors.forename: Xing-bo
authors.initials: XB
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Bing
authors.initials: B
authors.lastname: Shen
authors.forename: Zong-ke
authors.initials: ZK
authors.lastname: Zhou
authors.forename: Fu-xing
authors.initials: FX
authors.lastname: Pei
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D019902:Chondrocytes / Q000187:drug effects / Q000473:pathology; D004032:Diet / Q000009:adverse effects*; D004838:Epiphyses / Q000187:drug effects*; D005260:Female; D006132:Growth Plate / Q000187:drug effects*; D006614:Hindlimb; D008297:Male; D009336:Necrosis / Q000139:chemically induced; D009748:Nutrition Disorders; D051381:Rats; D017208:Rats, Wistar; D013605:T-2 Toxin / Q000633:toxicity*
chemical_list: D013605:T-2 Toxin