Quantifying the exploratory behaviour of Amphibalanus amphitrite cyprids.
The behavioural response of cypris larvae from A. amphitrite (=Balanus amphitrite) exploring three model glass surfaces is quantified by close-range microscopy. Step length and step duration measurements reveal a response to both surface properties and flow. Without flow, 2-day-old cyprids took larger steps with shorter step duration on hydrophilic glass surfaces (bare and NH2-treated) vs hydrophobic glass (CH3-treated). These parameters suggest a more detailed, local inspection of hydrophobic surfaces and a more extensive exploration for hydrophilic surfaces. Cyprids under flow took longer steps and exhibited shorter probing times on hydrophobic glass. On hydrophilic glass, cyprids increased their step duration under flow. This active response is attributed to drag and lift forces challenging the cyprids' temporary anchoring to the substratum. Seven-day-old cyprids showed almost no discrimination between the model surfaces. Microscopic-scale observation of cyprid exploration is expected to provide new insights into interactions between cyprids and surfaces.
Chaw
Kuan Chun
KC
Institute of Materials Research and Engineering, (A*STAR) Agency for Science, Technology and Research, Singapore, Singapore.
Birch
William R
WR
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D057219:Biofouling / Q000517:prevention & control; D001681:Biological Assay; D005898:Glass; D057927:Hydrophobic and Hydrophilic Interactions; D007814:Larva / Q000502:physiology; D008386:Marine Biology; D009068:Movement; D013499:Surface Properties; D001468:Thoracica / Q000254:growth & development / Q000502:physiology