title: Ovicidal and larvicidal activity of crude extracts of Melia azedarach against Haemonchus contortus (Strongylida).
abstract: The rapid development of anthelmintic resistance, associated with the high cost of the available anthelmintic drugs, has limited the success of gastrointestinal nematodiosis control in sheep and goats and thus created interest in studying medicinal plants as an alternative source of anthelmintics. The aim of this study was carried out to evaluate the anthelmintic activity of the leaves and seed aqueous and hydro-alcoholic extracts of Melia azedarach L. (Meliaceae) were tested for in vitro ovicidal and larvicidal activity against Haemonchus contortus (Strongylida). Both extracts were evaluated at five concentrations: 12.5, 6.2, 3.12, 1.56, and 0.78 mg/ml. The leaves aqueous and hydro-alcoholic extracts inhibited 99.4% and 100% of the egg hatching and 100% of larval development at 12.5 mg/ml, respectively. In a similar way, the leaves hydro-alcoholic extract was the most active on egg inhibition (ED (50) = 1.97 and ED ( 90 ) = 5.05 mg/ml), leaves and seed aqueous and hydro-alcoholic extracts showed the best inhibition of larval development (ED ( 50 ) = 3.01, 2.43, 3.17, 2.40, and ED ( 90 ) = 10.53, 8.14, 11.94, and 8.19 mg/ml), respectively. These results suggest that utilization of M. azedarach extracts is useful in the control of H. contortus.
authors.affiliation: Unit of Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509 Vellore District, Tamil Nadu, India.
authors.forename: Chinnaperumal
authors.initials: C
authors.lastname: Kamaraj
authors.forename: Abdul Abdul
authors.initials: AA
authors.lastname: Rahuman
authors.forename: Asokan
authors.initials: A
authors.lastname: Bagavan
authors.forename: Mohamed Jamal
authors.initials: MJ
authors.lastname: Mohamed
authors.forename: Gandhi
authors.initials: G
authors.lastname: Elango
authors.forename: Govindasamy
authors.initials: G
authors.lastname: Rajakumar
authors.forename: Abdul Abduz
authors.initials: AA
authors.lastname: Zahir
authors.forename: Thirunavukkarasu
authors.initials: T
authors.lastname: Santhoshkumar
authors.forename: Sampath
authors.initials: S
authors.lastname: Marimuthu
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000302:isolation & purification / Q000494:pharmacology*; D045424:Complex Mixtures / Q000302:isolation & purification / Q000494:pharmacology*; D006189:Haemonchus / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D031586:Melia azedarach / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D016019:Survival Analysis
chemical_list: D000871:Anthelmintics; D045424:Complex Mixtures