title: Data mining cDNAs reveals three new single stranded RNA viruses in Nasonia (Hymenoptera: Pteromalidae).
abstract: We report three novel small RNA viruses uncovered from cDNA libraries from parasitoid wasps in the genus Nasonia. The genome of this kind of virus is a positive-sense single-stranded RNA with a 3' poly(A), which facilitates cloning from cDNAs. Two of the viruses, NvitV-1 and NvitV-2, possess a RNA-dependent RNA polymerase that associates them with the family Iflaviridae of the order Picornavirales. A third virus, NvitV-3, is most similar to the Nora virus from Drosophila. A reverse transcription-PCR method developed for NvitV-1 indicates that it is a persistent commensal infection of Nasonia.
authors.affiliation: Department of Biology, University of Rochester, Rochester, NY 14627, USA.
authors.forename: D C S G
authors.initials: DC
authors.lastname: Oliveira
authors.forename: W B
authors.initials: WB
authors.lastname: Hunter
authors.forename: J
authors.initials: J
authors.lastname: Ng
authors.forename: C A
authors.initials: CA
authors.lastname: Desjardins
authors.forename: P M
authors.initials: PM
authors.lastname: Dang
authors.forename: J H
authors.initials: JH
authors.lastname: Werren
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D057225:Data Mining; D020224:Expressed Sequence Tags; D015723:Gene Library; D008969:Molecular Sequence Data; D010802:Phylogeny; D012328:RNA Viruses / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014863:Wasps / Q000821:virology*