Sporosalibacterium faouarense gen. nov., sp. nov., a moderately halophilic bacterium isolated from oil-contaminated soil.
A novel strictly anaerobic, moderately halophilic and mesophilic bacterium, designated strain SOL3f37(T), was isolated from a hydrocarbon-polluted soil surrounding a deep petroleum environment located in south Tunisia. Cells of strain SOL3f37(T) stained Gram-positive and were motile, straight and spore-forming. Strain SOL3f37(T) had a typical Gram-positive-type cell-wall structure, unlike the thick, multilayered cell wall of its closest relative Clostridiisalibacter paucivorans. The major fatty acids were iso-C(15 : 0) (41 %), iso-C(14 : 0) 3-OH and/or iso-C(15 : 0) dimethyl acetal (21.6 %), iso-C(13 : 0) (4.4 %), anteiso-C(15 : 0) (3.9 %) and iso-C(15 : 1) (2.8 %). Strain SOL3f37(T) grew between 20 and 48 °C (optimum 40 °C) and at pH 6.2-8.1 (optimum pH 6.9). Strain SOL3f37(T) required at least 0.5 NaCl l(-1) and grew in the presence of NaCl concentrations up to 150 g l(-1) (optimum 40 g l(-1)). Yeast extract (2 g l(-1)) was required for degradation of pyruvate, fumarate, fructose, glucose and mannitol. Also, strain SOL3f37(T) grew heterotrophically on yeast extract, peptone and bio-Trypticase, but was unable to grow on Casamino acids. Sulfate, thiosulfate, sulfite, elemental sulfur, fumarate, nitrate and nitrite were not reduced. The DNA G+C content was 30.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SOL3f37(T) was a member of the family Clostridiaceae in the order Clostridiales; strain SOL3f37(T) was related to members of various genera of the family Clostridiaceae. It exhibited highest 16S rRNA gene sequence similarity (93.4 %) with Clostridiisalibacter paucivorans 37HS60(T), 91.8 % with Thermohalobacter berrensis CTT3(T) and 91.7 % with Caloranaerobacter azorensis MV1087(T). On the basis of genotypic, phenotypic and phylogenetic data, it is suggested that strain SOL3f37(T) represents a novel species in a new genus. The name Sporosalibacterium faouarense gen. nov., sp. nov. is proposed, with SOL3f37(T) (=DSM 21485(T) =JCM 15487(T)) as the type strain of Sporosalibacterium faouarense.
Rezgui
Raja
R
Laboratoire de Microbiologie, Groupe des Bioprocédés, Institut Pasteur de Tunis, BP 74, Place Pasteur, Belvédère, Tunis 1002, Tunisia.|Microbiologie et Biotechnologie des Environnements Chauds, UMR D180, IRD, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
Ben Ali Gam
Zouhaier
Z
Microbiologie et Biotechnologie des Environnements Chauds, UMR D180, IRD, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
Ben Hamed
Said
S
Laboratoire de Microbiologie, Groupe des Bioprocédés, Institut Pasteur de Tunis, BP 74, Place Pasteur, Belvédère, Tunis 1002, Tunisia.|Microbiologie et Biotechnologie des Environnements Chauds, UMR D180, IRD, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
Fardeau
Marie-Laure
ML
Microbiologie et Biotechnologie des Environnements Chauds, UMR D180, IRD, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
Cayol
Jean-Luc
JL
Microbiologie et Biotechnologie des Environnements Chauds, UMR D180, IRD, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
Maaroufi
Abderrazak
A
Laboratoire de Microbiologie, Groupe des Bioprocédés, Institut Pasteur de Tunis, BP 74, Place Pasteur, Belvédère, Tunis 1002, Tunisia.
Labat
Marc
M
Microbiologie et Biotechnologie des Environnements Chauds, UMR D180, IRD, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008969:Molecular Sequence Data; D010578:Petroleum; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants; D013696:Temperature; D014416:Tunisia
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D012965:Sodium Chloride