title: Terpenoids induce cell cycle arrest and apoptosis from the stems of Celastrus kusanoi associated with reactive oxygen species.
abstract: Bioguided fractionation of the CHCl(3) extracts obtained from Celastrus kusanoi stems led to isolation of two new terpenoids, 3beta-hydroxy-11,14-oxo-abieta-8,12-diene (1) and 3beta-trans-(3,4-dihydroxycinnamoyloxy)-11alpha-methoxy-12-ursene (2), and four known compounds characterized by spectroscopic methods. Compounds 1 and 2 and known triterpenoid erythrodiol (3) exhibited cytotoxic activity against bladder cancer cells (NTUB1) with IC(50) values of 58.2 +/- 2.3, 160.1 +/- 60.9, and 18.3 +/- 0.5 microM, respectively. Exposure of NTUB1 to 3 (5 and 10 microM) for 24 h significantly increased the level of production of reactive oxygen species (ROS). Flow cytometric analysis showed that treatment of NTUB1 with 3 led to the cell cycle arrest at G0/G1 accompanied by an increase in the extent of apoptotic cell death after 24 h. These data suggest that the presentation of G1 phase arrest and apoptosis in 3-treated NTUB1 for 24 h was mediated through an increased amount of ROS in cells exposed to 3.
authors.affiliation: Faculty of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Chen
authors.forename: Kai-Wei
authors.initials: KW
authors.lastname: Lin
authors.forename: A-Mei
authors.initials: AM
authors.lastname: Huang
authors.forename: Huang-Yao
authors.initials: HY
authors.lastname: Tu
authors.forename: Bai-Luh
authors.initials: BL
authors.lastname: Wei
authors.forename: Tzyh-Chyuan
authors.initials: TC
authors.lastname: Hour
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Yen
authors.forename: Yeong-Shiou
authors.initials: YS
authors.lastname: Pu
authors.forename: Chun-Nan
authors.initials: CN
authors.lastname: Lin
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D031247:Celastrus / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013729:Terpenes / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013729:Terpenes