title: Investigation of the europium emission spectra of the europium-oxytetracycline complex in the presence of human low-density lipoproteins.
abstract: Low-Density Lipoprotein (LDL), often known as "bad cholesterol" is one of the responsible to increase the risk of coronary arterial diseases. For this reason, the cholesterol present in the LDL particle has become one of the main parameters to be quantified in routine clinical diagnosis. A number of tools are available to assess LDL particles and estimate the cholesterol concentration in the blood. The most common methods to quantify the LDL in the plasma are the density gradient ultracentrifugation and nuclear magnetic resonance (NMR). However, these techniques require special equipments and can take a long time to provide the results. In this paper, we report on the increase of the Europium emission in Europium-oxytetracycline complex aqueous solutions in the presence of LDL. This increase is proportional to the LDL concentration in the solution. This phenomenum can be used to develop a method to quantify the number of LDL particles in a sample. A comparison between the performances of the oxytetracycline and the tetracycline in the complexes is also made.
authors.affiliation: Centro de Lasers e Aplicações, IPEN/CNEN-SP, São Paulo, SP, Brazil.
authors.forename: Luciane dos Santos
authors.initials: Ldos S
authors.lastname: Teixeira
authors.forename: Andréa Nastri
authors.initials: AN
authors.lastname: Grasso
authors.forename: Andrea Moreira
authors.initials: AM
authors.lastname: Monteiro
authors.forename: Antonio M Figueiredo
authors.initials: AM
authors.lastname: Neto
authors.forename: Nilson Dias
authors.initials: ND
authors.lastname: Vieira
authors.forename: Magnus
authors.initials: M
authors.lastname: Gidlund
authors.forename: Juliana
authors.initials: J
authors.lastname: Steffens
authors.forename: Lilia Coronato
authors.initials: LC
authors.lastname: Courrol
mesh_terms: D002784:Cholesterol / Q000097:blood; D005063:Europium / Q000737:chemistry*; D006801:Humans; D008077:Lipoproteins, LDL / Q000032:analysis* / Q000097:blood; D008722:Methods; D010118:Oxytetracycline / Q000737:chemistry*; D012996:Solutions; D013057:Spectrum Analysis
chemical_list: D008077:Lipoproteins, LDL; D012996:Solutions; D005063:Europium; D002784:Cholesterol; D010118:Oxytetracycline