Fluorescent quantum dot-labeled aptamer bioprobes specifically targeting mouse liver cancer cells.
Fluorophore-labeled bioprobes are the key for fluorescent-labeled imaging technology. In the present work, mouse liver hepatoma cell line BNL 1ME A.7R.1 (MEAR)-specific ssDNA aptamer TLS9a was used to fabricate quantum dot-labeled aptamer bioprobe (QD-Apt), which was obtained by conjugating streptavidin-modified quantum dots (SA-QDs) with biotin-derived aptamer via the interaction between biotin and streptavidin. The QD-Apt was of monodispersity and excellent fluorescence properties. When the optimum ratio of SA-QDs to aptamer, which is 1:16, was used in the preparation of the QD-Apt, the resultant QD-Apt was of satisfactory bioactivity. They could specifically recognize MEAR cells and could not recognize BNL cells and Hela cells. Particularly, the growth and viability of QD-Apt bound MEAR cells were not affected by QD-Apt within 84 h compared to control cells, indicating that the probe was biocompatible and suitable for live cell imaging.
Zhang
Jing
J
School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
Jia
Xing
X
Lv
Xiao-Jing
XJ
Deng
Yu-Lin
YL
Xie
Hai-Yan
HY
D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001710:Biotin / Q000378:metabolism; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D015335:Molecular Probes / Q000737:chemistry* / Q000378:metabolism*; D045663:Quantum Dots; D015203:Reproducibility of Results; D013194:Staining and Labeling; D019809:Streptavidin / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
D052157:Aptamers, Nucleotide; D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D015335:Molecular Probes; D001710:Biotin; D019809:Streptavidin