title: Singlepath Salmonella. Performance Tested Method 060401.
abstract: Singlepath Salmonella is an immunochromatographic (lateral flow) assay for the presumptive qualitative detection of Salmonella spp. in food. A previous AOAC Performance Tested Method study evaluated Singlepath Salmonella as an effective method for the detection of Salmonella spp. in the following selected foods: dried skimmed milk, black pepper, dried pet food, desiccated coconut, cooked peeled frozen prawns, raw ground beef, and raw ground turkey. In this Emergency Response Validation extension, creamy peanut butter was inoculated with S. enterica. ser. Typhimurium. For low contamination level (1.08 CFU/25 g), a Chi-square value of 0.5 indicated that there was no significant difference between Singlepath Salmonella and the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA-BAM) reference method. For high-level and uninoculated control there was 100% agreement between the methods.
authors.affiliation: Merck KGaA, Frankfurter Strasse 250, 64293 Darmstadt, Germany. charlotte.lindhardt@merck.de
authors.forename: Charlotte
authors.initials: C
authors.lastname: Lindhardt
authors.forename: Holger
authors.initials: H
authors.lastname: Schönenbrücher
authors.forename: Jörg
authors.initials: J
authors.lastname: Slaghuis
authors.forename: Andreas
authors.initials: A
authors.lastname: Bubert
authors.forename: Rolf
authors.initials: R
authors.lastname: Ossmer
mesh_terms: D010367:Arachis / Q000382:microbiology; D003470:Culture Media; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D015203:Reproducibility of Results; D012475:Salmonella / Q000737:chemistry*; D019779:Salmonella enterica / Q000737:chemistry / Q000254:growth & development
chemical_list: D003470:Culture Media; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic