Direct culture-independent Strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis.
We examined if multilocus sequence typing (MLST), a method for genotyping and species identification of Burkholderia cepacia complex bacteria, could be applied directly to cystic fibrosis sputum. The redesigned nested-PCR MLST format was successfully used to accurately identify strains in 23 sputum samples, of which 8 were culture negative.
Drevinek
Pavel
P
Department of Pediatrics, 2nd Medical School, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic. pavel.drevinek@Lfmotol.cuni.cz
Vosahlikova
Sarka
S
Dedeckova
Klara
K
Cinek
Ondrej
O
Mahenthiralingam
Eshwar
E
D015373:Bacterial Typing Techniques / Q000379:methods*; D019121:Burkholderia Infections / Q000382:microbiology*; D042602:Burkholderia cepacia complex / Q000145:classification* / Q000235:genetics; D016000:Cluster Analysis; D003550:Cystic Fibrosis / Q000150:complications*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D018410:Pneumonia, Bacterial / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology*
D004269:DNA, Bacterial