Image patterned molecular delivery into live cells using gold particle coated substrates.
An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.
Wu
Ting-Hsiang
TH
Department of Electrical Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, 48-121 Engineering IV, Los Angeles, CA 90095-1597, USA.
Kalim
Sheraz
S
Callahan
Caitlin
C
Teitell
Michael A
MA
Chiou
Pei-Yu
PY
D002462:Cell Membrane / Q000737:chemistry* / Q000528:radiation effects; D004058:Diffusion / Q000528:radiation effects; D016503:Drug Delivery Systems / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry* / Q000528:radiation effects; D006046:Gold / Q000737:chemistry* / Q000528:radiation effects*; D006367:HeLa Cells; D006801:Humans; D007668:Kidney / Q000737:chemistry* / Q000528:radiation effects; D008027:Light; D052898:Optical Tweezers
D005452:Fluoresceins; D006046:Gold; C007740:fluorexon