Singlepath Salmonella. Performance Tested Method 060401.
Singlepath Salmonella is an immunochromatographic (lateral flow) assay for the presumptive qualitative detection of Salmonella spp. in food. A previous AOAC Performance Tested Method study evaluated Singlepath Salmonella as an effective method for the detection of Salmonella spp. in the following selected foods: dried skimmed milk, black pepper, dried pet food, desiccated coconut, cooked peeled frozen prawns, raw ground beef, and raw ground turkey. In this Emergency Response Validation extension, creamy peanut butter was inoculated with S. enterica. ser. Typhimurium. For low contamination level (1.08 CFU/25 g), a Chi-square value of 0.5 indicated that there was no significant difference between Singlepath Salmonella and the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA-BAM) reference method. For high-level and uninoculated control there was 100% agreement between the methods.
Lindhardt
Charlotte
C
Merck KGaA, Frankfurter Strasse 250, 64293 Darmstadt, Germany. charlotte.lindhardt@merck.de
Schönenbrücher
Holger
H
Slaghuis
Jörg
J
Bubert
Andreas
A
Ossmer
Rolf
R
D010367:Arachis / Q000382:microbiology; D003470:Culture Media; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D015203:Reproducibility of Results; D012475:Salmonella / Q000737:chemistry*; D019779:Salmonella enterica / Q000737:chemistry / Q000254:growth & development
D003470:Culture Media; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic