Tumour cell lines HT-29 and FaDu produce proinflammatory cytokines and activate neutrophils in vitro: possible applications for neutrophil-based antitumour treatment.
There is evidence that polymorphonuclear neutrophils (PMNs) can exert severe antineoplastic effects. Cross-talk between tumour cells and endothelial cells (ECs) is necessary for the accumulation of PMN around a tumour. This work reports the ability of two PMN-sensitive, human, permanent cell lines-colorectal adenocarcinoma (HT-29) and pharyngeal squamous-cell carcinoma (FaDu) cells-to act as inflammatory foci. PMNs were cytotoxic to both lines, the adhesion of the PMNs to the tumour cells being important in this effect. The tumour cells released appreciable amounts of IL-8 and GROalpha, and induced the transmigration of PMN through human microvascular-EC monolayers. Conditioning media associated with both lines induced the adhesion of PMN and the surface expression of ICAM-1 in microvascular-EC. In addition, FaDu-conditioning-medium strongly induced the production of proinflammatory cytokines by microvascular-EC. These results support the idea that tumour cells might normally induce a potent acute inflammatory response, leading to their own destruction.
Brú
Antonio
A
Applied Mathematics Department, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Souto
Juan-Carlos
JC
Alcolea
Sonia
S
Antón
Rosa
R
Remacha
Angel
A
Camacho
Mercedes
M
Soler
Marta
M
Brú
Isabel
I
Porres
Amelia
A
Vila
Luis
L
D015153:Blotting, Western; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D054360:Chemokine CXCL1 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D005434:Flow Cytometry; D019073:HT29 Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D009369:Neoplasms / Q000276:immunology* / Q000378:metabolism*; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D053482:beta 2-Glycoprotein I / Q000378:metabolism
D054360:Chemokine CXCL1; D016207:Cytokines; D016209:Interleukin-8; D053482:beta 2-Glycoprotein I; D018799:Intercellular Adhesion Molecule-1