Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor.
We investigated the effects of different concentrations of auxin and auxin/cytokinin combinations, inoculum sizes and Murashige and Skoog (MS) medium dilutions on biomass and accumulation of total phenols and flavonoids in adventitious roots of Hypericum perforatum. The optimized conditions for the shake flask culture growth and secondary metabolites production were 0.1mg/l kinetin with 1mg/l indole-3-butyric acid (IBA), an inoculum density of 6g/l fresh weight (FW) and 1/2MS in the 5-week old cultures. By inoculating 6g/l of adventitious roots into a 3-l balloon type bubble bioreactor (BTBB) containing l/2 MS medium supplemented with 0.1mg/l kinetin with 1mg/l IBA, 104.2g/l adventitious roots were harvested containing 56.47+/-0.22 mg/g dry weight (DW) total phenolics, 35.01+/-1.26 mg/g DW flavonoids, 0.97+/-0.06 mg/g DW chlorogenic acid and 1.389+/-0.80 mg/g DW hypericin. The results of our study contribute to optimization and development of bioreactor technology for adventitious root cultures of H. perforatum for the production of hypericin.
Cui
Xi-Hua
XH
Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Chungju 361-763, South Korea.
Chakrabarty
Debasis
D
Lee
Eun-Jung
EJ
Paek
Kee-Yoeup
KY
D000838:Anions / Q000494:pharmacology; D018533:Biomass; D019149:Bioreactors; D020148:Butyric Acid / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D003583:Cytokinins / Q000494:pharmacology; D004553:Electric Conductivity; D005419:Flavonoids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D020902:Hypericum / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000494:pharmacology; D010636:Phenols / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development*; D013997:Time Factors
D000838:Anions; D002412:Cations; D003470:Culture Media; D003583:Cytokinins; D005419:Flavonoids; D007210:Indoleacetic Acids; D010636:Phenols; D020148:Butyric Acid