Bioglass as a carrier for reindeer bone protein extract in the healing of rat femur defect.
Bioactive glasses have been developed as scaffolds for bone tissue engineering but combination with reindeer bone protein extract has not been evaluated. We investigated the effects of bone protein extract implants (5-40 mg dosages) with bioglass (BG) carrier on the healing of rat femur defects. Bioglass implants and untreated defects served as controls. All doses of extract increased bone formation compared with the control groups, and bone union was enhanced with doses of 10 mg or more. In comparison with untreated defect, mean cross-sectional bone area at the defect site was greater when implants with BG + 15 mg of extract or bioglass alone were used, bone density at the defect site was higher in all bioglass groups with and without bone extract, and the BG + 15 mg extract dosage marginally increased bone torsional stiffness in mechanical testing. Bioglass performed well as a carrier candidate for reindeer bone protein extract.
Tölli
Hanna
H
Department of Orthopaedic and Trauma Surgery, University of Oulu, FIN-90014, Oulu, Finland. hanna.tolli@bbs-artebone.fi
Kujala
Sauli
S
Levonen
Katri
K
Jämsä
Timo
T
Jalovaara
Pekka
P
D000818:Animals; D015519:Bone Density / Q000187:drug effects; D002516:Ceramics; D005269:Femur / Q000187:drug effects; D008297:Male; D011506:Proteins / Q000008:administration & dosage* / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012053:Reindeer; D023822:Tissue Engineering; D014945:Wound Healing / Q000187:drug effects*
C022107:Bioglass; D011506:Proteins