Exogenous allogenic fragmented double-stranded DNA is internalized into human dendritic cells and enhances their allostimulatory activity.
Exogenous allogenic DNA as nucleosome-free fragments reaches main cellular compartments (cytoplasm, nucleus) of human dendritic cells and deposits in the nuclear interchromosomal space without visibly changing in linear size. The presence of such allogenic fragmented DNA in medium in which human dendritic cells are cultured produces an enhancement of their allostimulatory activity. This enhancement is comparable to that produced by the standard maturation stimulus lipopolysaccharide Escherichia coli.
Alyamkina
Ekaterina A
EA
Novosibirsk State University, Novosibirsk, Russia.
Dolgova
Evgenia V
EV
Likhacheva
Anastasia S
AS
Rogachev
Vladimir A
VA
Sebeleva
Tamara E
TE
Nikolin
Valeriy P
VP
Popova
Nelly A
NA
Kiseleva
Elena V
EV
Orishchenko
Konstantin E
KE
Sakhno
Ludmila V
LV
Gel'fgat
Evgeniy L
EL
Ostanin
Alexandr A
AA
Chernykh
Elena R
ER
Zagrebelniy
Stanislav N
SN
Bogachev
Sergey S
SS
Shurdov
Mikhail A
MA
D000818:Animals; D004247:DNA / Q000276:immunology* / Q000648:ultrastructure; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004705:Endocytosis / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D051379:Mice
D004247:DNA