title: A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation.
abstract: We describe a new method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins.
authors.affiliation: Institute of Microbiology, Department of Biology, Eidgenössische Technische Hochschule (ETH) Zurich, Zurich, Switzerland.
authors.forename: Flavio
authors.initials: F
authors.lastname: Schwarz
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Cishan
authors.initials: C
authors.lastname: Li
authors.forename: Benjamin L
authors.initials: BL
authors.lastname: Schulz
authors.forename: Christian
authors.initials: C
authors.lastname: Lizak
authors.forename: Alessandro
authors.initials: A
authors.lastname: Palumbo
authors.forename: Shin
authors.initials: S
authors.lastname: Numao
authors.forename: Dario
authors.initials: D
authors.lastname: Neri
authors.forename: Markus
authors.initials: M
authors.lastname: Aebi
authors.forename: Lai-Xi
authors.initials: LX
authors.lastname: Wang
mesh_terms: D016123:Campylobacter jejuni / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D056890:Eukaryota / Q000737:chemistry / Q000378:metabolism*; D005818:Genetic Engineering; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry; D006031:Glycosylation
chemical_list: D006023:Glycoproteins