title: Cytotoxic ent-kaurane diterpenoids from Isodon macrophyllus.
abstract: Two new ent-kaurane diterpenoids, dayecrystals D-E (1-2), together with nine known compounds, isojaponin A (3), rabdosin A (4), lushanrubescensin J (5), wikstroemioidin B (6), maoyecrystal C (7), rabdosin B (8), isodonal (9), shikokianin (10), and effusanin A (11), were isolated from the leaves of Isodon macrophyllus. The structures of the new compounds were elucidated using 1D and 2D NMR spectroscopy. The (13)C-NMR spectral data of compound 4 are reported for the first time. All of the compounds were tested for their cytotoxicities against DU145 and LoVo human tumor cells. Compounds 4, 10, and 11 showed inhibitory effects on DU145 cells with IC(50) values 5.90, 4.24, and 3.16 microM, and LoVo cells with IC(50) values 14.20, 17.55, and 3.02 microM, respectively.
authors.affiliation: Pharmaceutical Laboratory, Xinxiang Medical University, Xinxiang, Henan Province, China.
authors.forename: Ji-Xia
authors.initials: JX
authors.lastname: Zhang
authors.forename: Zhi-An
authors.initials: ZA
authors.lastname: He
authors.forename: Zheng-Yue
authors.initials: ZY
authors.lastname: Chen
authors.forename: Yong-Xue
authors.initials: YX
authors.lastname: Wang
authors.forename: Su-Ping
authors.initials: SP
authors.lastname: Bai
authors.forename: Han-Dong
authors.initials: HD
authors.lastname: Sun
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D037602:Isodon / Q000737:chemistry*; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; C547615:dayecrystal D; C547616:dayecrystal E; C116235:shikokianin; C037017:rabdosin A; C037018:rabdosin B