Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid.
We demonstrate, using transmission electron microscopy and immunopurification with an antibody specific for RNA/DNA hybrid, that intact mitochondrial DNA replication intermediates are essentially duplex throughout their length but contain extensive RNA tracts on one strand. However, the extent of preservation of RNA in such molecules is highly dependent on the preparative method used. These findings strongly support the strand-coupled model of mitochondrial DNA replication involving RNA incorporation throughout the lagging strand.
Pohjoismäki
Jaakko L O
JL
Department of Forensic Medicine and Tampere University Hospital, FI-33014 University of Tampere, Finland.
Holmes
J Bradley
JB
Wood
Stuart R
SR
Yang
Ming-Yao
MY
Yasukawa
Takehiro
T
Reyes
Aurelio
A
Bailey
Laura J
LJ
Cluett
Tricia J
TJ
Goffart
Steffi
S
Willcox
Smaranda
S
Rigby
Rachel E
RE
Jackson
Andrew P
AP
Spelbrink
Johannes N
JN
Griffith
Jack D
JD
Crouch
Robert J
RJ
Jacobs
Howard T
HT
Holt
Ian J
IJ
D000818:Animals; D004247:DNA; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000737:chemistry*; D006801:Humans; D008322:Mammals; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012313:RNA
D004272:DNA, Mitochondrial; D012313:RNA; D004247:DNA