title: Effect of chromium and cobalt ions on the expression of antioxidant enzymes in human U937 macrophage-like cells.
abstract: The main concern associated with metal-on-metal (MM) hip prosthesis is the presence of metal ions, mainly chromium (Cr) and cobalt (Co), which are found both systemically and locally in the organism of patients. Previous studies revealed that Cr(III) and Co(II) ions could induce damages to proteins in macrophage-like cells in vitro, probably through the formation of reactive oxygen species (ROS). We then hypothesized that these ions can modify the expression of antioxidant enzymes in these cells. Results showed that Cr(VI) induced the protein expression of Mn-superoxide dismutase, Cu/Zn-superoxide dismutase, catalase, glutathione peroxidase, and heme oxygenase-1 (HO-1) but had no effect of the expression of their mRNA. Cr(III) have no effect on the expression of all these antioxidant enzymes. Co(II) induced the expression of both protein and mRNA of HO-1 only. In conclusion, results showed that Cr(VI), Cr(III), and Co(II) had differential effects on the expression of antioxidant enzymes in macrophage-like cells in vitro.
authors.affiliation: Department of Biomedical Engineering, McGill University, Duff Medical Building, 3775 University Street, Montreal, Quebec H3A 2B4, Canada.
authors.forename: Cathy
authors.initials: C
authors.lastname: Tkaczyk
authors.forename: Olga L
authors.initials: OL
authors.lastname: Huk
authors.forename: Fackson
authors.initials: F
authors.lastname: Mwale
authors.forename: John
authors.initials: J
authors.lastname: Antoniou
authors.forename: David J
authors.initials: DJ
authors.lastname: Zukor
authors.forename: Alain
authors.initials: A
authors.lastname: Petit
authors.forename: Maryam
authors.initials: M
authors.lastname: Tabrizian
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002857:Chromium / Q000737:chemistry / Q000494:pharmacology*; D003035:Cobalt / Q000737:chemistry / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007477:Ions / Q000494:pharmacology*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D020298:U937 Cells
chemical_list: D000975:Antioxidants; D007477:Ions; D007527:Isoenzymes; D017382:Reactive Oxygen Species; D002857:Chromium; D003035:Cobalt; D002374:Catalase; D005979:Glutathione Peroxidase; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase