title: Cell uptake of Zn(II)-phthalocyanine-containing liposomes by clathrin-mediated endocytosis.
abstract: The study of uptake mechanisms of therapeutic drugs has a growing interest in biomedical research. In this work the cell uptake and phototoxicity of the photosensitizer Zn(II)-phthalocyanine (ZnPc) in dipalmitoyl-phosphatidyl-choline liposomes have been studied in the presence or absence of inhibitors of macropinocytosis (cytochalasin D), and clathrin-mediated endocytosis (dynasore). No differences in the uptake or photodynamic damage were observed in A-549 cells subjected to incubation with either ZnPc alone or in combination with cytochalasin D. On the contrary, co-incubation of A-549 cells with ZnPc and dynasore resulted in a significant decrease of photodamage as well as negligible uptake of the photosensitizer. These results indicate that ZnPc is internalized into cells preferentially by a mechanism of clathrin-mediated endocytosis.
authors.affiliation: Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Darwin 2, 28049, Madrid, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Soriano
authors.forename: Juan C
authors.initials: JC
authors.lastname: Stockert
authors.forename: Angeles
authors.initials: A
authors.lastname: Villanueva
authors.forename: Magdalena
authors.initials: M
authors.lastname: Cañete
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000494:pharmacology; D001692:Biological Transport; D002477:Cells / Q000378:metabolism; D022082:Cellular Structures / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism*; D015638:Cytochalasin D / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects*; D007211:Indoles / Q000378:metabolism*; D054833:Isoindoles; D008081:Liposomes / Q000494:pharmacology*; D009942:Organometallic Compounds / Q000378:metabolism*; D017319:Photosensitizing Agents / Q000494:pharmacology; D017967:Zinc Compounds
chemical_list: D002966:Clathrin; D007211:Indoles; D054833:Isoindoles; D008081:Liposomes; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D015638:Cytochalasin D; D015060:1,2-Dipalmitoylphosphatidylcholine; C013647:phthalocyanine