title: Universally Primed-PCR indicates geographical variation of Peronospora farinosa ex. Chenopodium quinoa.
abstract: In the Andean region of South America downy mildew, caused by Peronospora farinosa, is the most important disease of quinoa (Chenopodium quinoa). Peronospora farinosa, a highly polyphyletic species, occurs on quinoa and wild relatives on all continents. However, very little is known about the geographic diversity of the pathogen. As the interest in quinoa as a novel crop is increasing worldwide, geographical differences in the population structure of the downy mildew pathogen must be taken into consideration in order to design appropriate control strategies under a variety of circumstances. As a step towards understanding the geographic diversity of P. farinosa from quinoa, 40 downy mildew isolates from the Andean highlands and Denmark were characterized using universally primed PCR (UP-PCR). Eight UP-PCR primers were tested. A combined analysis of markers separated the Danish and Andean isolates in two distinct clusters. This study raises new questions about the origin and spread of P. farinosa on quinoa, its geographic diversity and host specificity.
authors.affiliation: Department of Plant Biology, Plant Pathology Section, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej, Frederiksberg C, Denmark.
authors.forename: Solveig
authors.initials: S
authors.lastname: Danielsen
authors.forename: Mette
authors.initials: M
authors.lastname: Lübeck
mesh_terms: D027465:Chenopodium quinoa / Q000382:microbiology*; D004271:DNA, Fungal / Q000235:genetics; D003718:Denmark; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D044742:Peronospora / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013020:South America
chemical_list: D004271:DNA, Fungal