Preparation and bioavailability assessment of SMEDDS containing valsartan.
A self-microemulsifying drug delivery system (SMEDDS) has been developed to enhance diffusion rate and oral bioavailability of valsartan. The solubility of valsartan was checked in different oils, surfactants, and cosurfactants and ternary phase diagrams were constructed to evaluate the microemulsion domain. The valsartan SMEDDS was prepared using Capmul MCM (oil), Tween 80 (surfactant), and polyethylene glycol 400 (cosurfactant). The particle size distribution, zeta potential, and polydispersity index were determined and were found to be 12.3 nm, -0.746, and 0.138, respectively. Diffusion rate of valsartan was measured by in vitro dialysis bag method using phosphate buffer pH 6.8 as diffusion media. Developed high-performance liquid chromatography method was used to determine drug content in diffusion media. Oral bioavailability of valsartan SMEDDS was checked by using rabbit model. Results of diffusion rate and oral bioavailability of valsartan SMEDDS were compared with those of pure drug solution and of marketed formulation. Diffusion of valsartan SMEDDS showed maximum drug release when compared to pure drug solution and marketed formulation. The area under curve and time showed significant improvement as the values obtained were 607 ng h/mL and 1 h for SMEDDS in comparison to 445.36 and 1.36 h for market formulation suggesting significant increase (p < 0.01) in oral bioavailability of valsartan SMEDDS.
Dixit
Adhvait R
AR
Pharmacy Department, The M.S. University of Baroda, Vadodara, Gujarat, India. adddixit@yahoo.co.in
Rajput
Sadhana J
SJ
Patel
Samir G
SG
D000818:Animals; D001682:Biological Availability; D002210:Caprylates; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D004058:Diffusion; D016503:Drug Delivery Systems / Q000379:methods*; D005989:Glycerides; D008297:Male; D009821:Oils / Q000737:chemistry; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D011817:Rabbits; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry; D013777:Tetrazoles; D014633:Valine / Q000031:analogs & derivatives; D000068756:Valsartan
D002210:Caprylates; D005989:Glycerides; D009821:Oils; D013501:Surface-Active Agents; D013777:Tetrazoles; D011092:Polyethylene Glycols; D000068756:Valsartan; D014633:Valine; C025343:monooctanoin