A pitfall in using BODIPY dyes to label lipid droplets for fluorescence microscopy.
The lipid droplet (LD) has become a focus of intense research. Fluorescence labeling is indispensable for the cell biological analysis of the LD, and a lipophilic fluorescence dye, BODIPY 493/503, which emits bright green fluorescence has been used extensively for LD labeling. The dye is convenient for double fluorescence labeling, but we noticed that it emits red fluorescence under certain conditions, which could lead to erroneous interpretations. We propose a protocol to preclude such a possibility.
Ohsaki
Yuki
Y
Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, 466-8550, Japan.
Shinohara
Yuki
Y
Suzuki
Michitaka
M
Fujimoto
Toyoshi
T
D000818:Animals; D001896:Boron Compounds / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000378:metabolism*; D002479:Inclusion Bodies / Q000737:chemistry; D008055:Lipids / Q000032:analysis; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods
C527198:4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene; C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D004396:Coloring Agents; D005456:Fluorescent Dyes; D008055:Lipids