Study of blood porphyrin spectral profile for diagnosis of chronic renal failure.
The progression to end-stage renal failure is independent of the initial pathogenic mechanism. Metabolic acidosis is a common consequence of chronic renal failure that results from inadequate ammonium excretion and decreased tubular bicarbonate reabsorption. Protoporphyrin IX (PpIX) is the immediate metabolic precursor of the heme molecule. The purpose of this study was to evaluate the levels of erythrocytes protoporphyrin IX at an animal model during progressive renal disease. A total of 36 eight-week-old male Wistar rats were divided into six groups: Normal, 4 and 8 weeks after 5/6 nephrectomy (NX). Renal function was evaluated by creatinine clearance and plasma creatinine levels. The autofluorescence of erythrocytes porphyrin of healthy and NX rats was analyzed using fluorescence spectroscopy. Emission spectra were obtained by exciting the samples at 405 nm. Significant differences between normal and NX rats autofluorescence shape occurred in the 600-700 nm spectral region. A correlation was observed between emission band intensity at 635 nm and progression of renal disease.
Tristão
Vivian Regina
VR
Disciplina Nefrologia, Departamento de Medicina, Universidade Federal de São Paulo-UNIFESP-EPM, São Paulo, Brazil.
de Carvalho
Fernando Felippe
FF
Gomes
Cinthia Zanini
CZ
Miranda
Adriana Regina
AR
Vequi-Suplicy
Cíntia C
CC
Lamy
Maria Teresa
MT
Schor
Nestor
N
Bellini
Maria Helena
MH
D000138:Acidosis / Q000097:blood / Q000473:pathology; D000818:Animals; D003404:Creatinine / Q000097:blood; D018450:Disease Progression; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000097:blood / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D009392:Nephrectomy; D011166:Porphyrins / Q000097:blood*; D011524:Protoporphyrins; D051381:Rats; D017208:Rats, Wistar
D011166:Porphyrins; D011524:Protoporphyrins; D003404:Creatinine; C028025:protoporphyrin IX