A new automated approach to determine monosodium glutamate in dehydrated broths by using the flow-batch methodology.
The advantages of the flow-batch methodology were exploited to implement a simple system with nephelometric detection for the determination of monosodium glutamate (MSG) in food samples. The method is based on the inhibitory effect of the MSG over the crystallization of L-lysine in an isopropanol/acetone mixture. The calibration curve was prepared on-line. The method was linear over the range of 2.8 x 10(-3) to 1.1 x 10(-2)gL(-1) and a detection limit of 9.7 x 10(-5)gL(-1) was achieved. It was successfully applied to determine the MSG concentration in food samples, without a previous treatment. A recovery study was carried out on real samples and the percentages were between 98 and 106%.
Acebal
Carolina C
CC
FIA Laboratory, Analytical Chemistry Section, INQUISUR (UNS-CONICET), Av. Alem 1253, B8000CPB Bahía Blanca, Buenos Aires, Argentina.
Insausti
Matías
M
Pistonesi
Marcelo F
MF
Lista
Adriana G
AG
Band
Beatriz S Fernández
BS
D001331:Automation; D005504:Food Analysis / Q000379:methods*; D007202:Indicators and Reagents / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D012970:Sodium Glutamate / Q000032:analysis*; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry*
D007202:Indicators and Reagents; D012997:Solvents; D014867:Water; D008239:Lysine; D012970:Sodium Glutamate