title: Identification of airborne bacterial and fungal species in the clinical microbiology laboratory of a university teaching hospital employing ribosomal DNA (rDNA) PCR and gene sequencing techniques.
abstract: Universal or "broad-range" PCR-based ribosomal DNA (rDNA) was performed on a collection of 58 isolates (n = 30 bacteria + 28 fungi), originating from environmental air from several locations within a busy clinical microbiology laboratory, supporting a university teaching hospital. A total of 10 bacterial genera were identified including both Gram-positive and Gram-negative genera. Gram-positive organisms accounted for 27/30 (90%) of total bacterial species, consisting of seven genera and included (in descending order of frequency) Staphylococcus, Micrococcus, Corynebacterium, Paenibacillus, Arthrobacter, Janibacter and Rothia. Gram-negative organisms were less frequently isolated 3/30 (10%) and comprised three genera, including Moraxella, Psychrobacter and Haloanella. Eight fungal genera were identified among the 28 fungal organisms isolated, including (in descending order of frequency) Cladosporium, Penicillium, Aspergillus, Thanatephorus, Absidia, Eurotium, Paraphaeosphaeria and Tritirachium, with Cladosporium accounting for 10/28 (35.7%) of the total fungal isolates. In conclusion, this study identified the presence of 10 bacterial and eight fungal genera in the air within the laboratory sampled. Although this reflected diversity of the microorganisms present, none of these organisms have been described previously as having an inhalational route of laboratory-acquired infection. Therefore, we believe that the species of organisms identified and the concentration levels of these airborne contaminants determined, do not pose a significant health and safety threat for immunocompotent laboratory personnel and visitors.
authors.affiliation: Belfast City Hospital, UK.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Nagano
authors.forename: Jim
authors.initials: J
authors.lastname: Walker
authors.forename: Anne
authors.initials: A
authors.lastname: Loughrey
authors.forename: Cherie
authors.initials: C
authors.lastname: Millar
authors.forename: Colin
authors.initials: C
authors.lastname: Goldsmith
authors.forename: Paul
authors.initials: P
authors.lastname: Rooney
authors.forename: Stuart
authors.initials: S
authors.lastname: Elborn
authors.forename: John
authors.initials: J
authors.lastname: Moore
mesh_terms: D000391:Air Microbiology; D001230:Aspergillus / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D019411:Clinical Laboratory Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics*; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006784:Hospitals, Teaching; D006785:Hospitals, University; D006801:Humans; D007753:Laboratories; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004271:DNA, Fungal; D004275:DNA, Ribosomal