Tribuli fructus constituents protect against tacrine-induced cytotoxicity in HepG2 cells.
A new phenolic amide, tribulusimide D (4-hydroxy-N-[3-(4-hydroxy-3-methoxyphenyl)-1-oxo-2-propen-1-yl]-3-methoxybenzamide) (1), together with a known phenolic amide, terrestriamide ((E)-3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydroxyphenyl)-2-oxoethyl]-prop-2-enamide) (2) and a flavonol glycoside, quercetin-3-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (3) were isolated from the H2O extract of Tribuli Fructus. Compounds 1 and 3 showed significant hepatoprotective activities, with EC50 values of 13.46 +/- 0.2 and 7.06 +/- 0.7 microM, respectively, against tacrine-induced cytotoxicity in HepG2 cells.
Byun
Erisa
E
Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570-749, Korea.
Jeong
Gil-Saeng
GS
An
Ren-Bo
RB
Min
Tae Sun
TS
Kim
Youn-Chul
YC
D002240:Carbohydrate Sequence; D002460:Cell Line; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D005638:Fruit / Q000737:chemistry; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D007094:Imides / Q000737:chemistry* / Q000494:pharmacology*; D008969:Molecular Sequence Data; D018697:Nootropic Agents / Q000633:toxicity*; D010936:Plant Extracts / Q000737:chemistry; D020011:Protective Agents / Q000737:chemistry* / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D013619:Tacrine / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D032444:Tribulus / Q000737:chemistry*
C549637:4-hydroxy-N-(3-(4-hydroxy-3-methoxyphenyl)-1-oxo-2-propen-1-yl)-3-methoxybenzamide; D007094:Imides; D018697:Nootropic Agents; D010936:Plant Extracts; D020011:Protective Agents; D013619:Tacrine; D006139:Guaiacol