A unique expression pattern of Tbx10 in the hindbrain as revealed by Tbx10(LacZ) allele.
To study the expression/function of Tbx10, a T-box gene, Tbx10(LacZ/+) mice were established by replacing the T-box coding region with a LacZ gene. X-gal staining showed that LacZ(+) cells were localized to two-cell populations in rhombomere 4 and rhombomere 6. No significant differences in the locations of LacZ(+) cells were found between Tbx10(LacZ/+) and Tbx10(LacZ/LacZ) mice, and the Tbx10(LacZ/LacZ) mice were viable and fertile. We found that the LacZ(+) cells are present in both embryonic and adult mice. Histological studies suggest that the rhombomere 4-derived LacZ(+) cells are a subpopulation of the ventral interneurons in the pons.
Xue
Xiao Dong
XD
Kimura
Wataru
W
Wang
Bo
B
Hikosaka
Keisuke
K
Itakura
Tatsuo
T
Uezato
Tadayoshi
T
Matsuda
Masashi
M
Koseki
Haruhiko
H
Miura
Naoyuki
N
D000483:Alleles; D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007395:Interneurons / Q000166:cytology / Q000378:metabolism; D007763:Lac Operon / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D009154:Mutation; D011149:Pons / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D012249:Rhombencephalon / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D020825:T-Box Domain Proteins / Q000235:genetics* / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D020825:T-Box Domain Proteins; C487130:Tbx10 protein, mouse; D001616:beta-Galactosidase