title: A pitfall in using BODIPY dyes to label lipid droplets for fluorescence microscopy.
abstract: The lipid droplet (LD) has become a focus of intense research. Fluorescence labeling is indispensable for the cell biological analysis of the LD, and a lipophilic fluorescence dye, BODIPY 493/503, which emits bright green fluorescence has been used extensively for LD labeling. The dye is convenient for double fluorescence labeling, but we noticed that it emits red fluorescence under certain conditions, which could lead to erroneous interpretations. We propose a protocol to preclude such a possibility.
authors.affiliation: Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, 466-8550, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ohsaki
authors.forename: Yuki
authors.initials: Y
authors.lastname: Shinohara
authors.forename: Michitaka
authors.initials: M
authors.lastname: Suzuki
authors.forename: Toyoshi
authors.initials: T
authors.lastname: Fujimoto
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000378:metabolism*; D002479:Inclusion Bodies / Q000737:chemistry; D008055:Lipids / Q000032:analysis; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods
chemical_list: C527198:4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene; C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D004396:Coloring Agents; D005456:Fluorescent Dyes; D008055:Lipids