title: Retinoic acid-inducible gene-I (RIG-I) is induced by IFN-{gamma} in human mesangial cells in culture: possible involvement of RIG-I in the inflammation in lupus nephritis.
abstract: Interferon-gamma is a potent Th1-type cytokine and a key molecule in the pathogenesis of autoimmune diseases including lupus nephritis. Retinoic acid-inducible gene-I is a putative RNA helicase that plays an important role in immune and inflammatory reactions. We previously demonstrated the increased expression of the retinoic acid-inducible gene-I protein in the kidney tissue of patients with lupus nephritis, and the presence of a significant amount of retinoic acid-inducible gene-I mRNA in the urinary sediment of patients with this inflammatory renal disease. In the present study, interferon-gamma was found to induce the expression of retinoic acid-inducible gene-I in human mesangial cells in culture. Knockdown of retinoic acid-inducible gene-I inhibited the interferon-gamma-induced upregulation of interferon regulatory factor 7, a transcriptional factor involved in immune and inflammatory reactions. These findings suggest that retinoic acid-inducible gene-I produced by mesangial cells may be involved in the pathogenesis of lupus nephritis.
authors.affiliation: Department of Vascular Biology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Imaizumi
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Tajima
authors.forename: K
authors.initials: K
authors.lastname: Tsuruga
authors.forename: E
authors.initials: E
authors.lastname: Oki
authors.forename: H
authors.initials: H
authors.lastname: Sashinami
authors.forename: T
authors.initials: T
authors.lastname: Matsumiya
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: I
authors.initials: I
authors.lastname: Inoue
authors.forename: E
authors.initials: E
authors.lastname: Ito
mesh_terms: D002478:Cells, Cultured; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000503:physiopathology; D050839:Interferon Regulatory Factor-7 / Q000235:genetics; D007371:Interferon-gamma / Q000378:metabolism*; D008181:Lupus Nephritis / Q000235:genetics / Q000503:physiopathology; D050527:Mesangial Cells / Q000378:metabolism*; D011971:Receptors, Immunologic; D015854:Up-Regulation
chemical_list: D050839:Interferon Regulatory Factor-7; D011971:Receptors, Immunologic; D007371:Interferon-gamma; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases