title: RapidChek SELECT Salmonella. Performance Tested Method 080601.
abstract: RapidChek SELECT Salmonella was previously validated in the Performance Tested Methods program for the detection of Salmonella spp. in raw ground chicken, chicken carcass rinse, sliced cooked turkey, and liquid eggs. The present matrix extension study conducted under the AOAC Research Institute Emergency Response Validation program compared the RapidChek SELECT Salmonella method to the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA-BAM) method for the detection of Salmonella Typhimurium in peanut butter. Overall, 27 samples were found positive by the RapidChek SELECT Salmonella method and 27 were found to be positive by the reference method. All RapidChek SELECT Salmonella presumptive positives were confirmed positive by the cultural reference method; additionally, all presumptive negative results were confirmed negative by the cultural reference method. Accordingly 0% false-negative rate and 0% false-positive rate were found. No significant difference between the RapidCheck SELECT Salmonella and FDA-BAM reference method was found; calculated Chi-square was 0. Results indicate that a low level of Salmonella in peanut butter can be successfully recovered and detected in the minimum 24 h enrichment protocol.
authors.affiliation: Strategic Diagnostics Inc., 128 Sandy Dr, Newark, DE 19713, USA.
authors.forename: Mark T
authors.initials: MT
authors.lastname: Muldoon
authors.forename: Jingkun
authors.initials: J
authors.lastname: Li
authors.forename: Meredith
authors.initials: M
authors.lastname: Sutzko
authors.forename: Ann Christine
authors.initials: AC
authors.lastname: Olsson-Allen
authors.forename: George
authors.initials: G
authors.lastname: Teaney
authors.forename: Verapaz
authors.initials: V
authors.lastname: Gonzalez
mesh_terms: D010367:Arachis / Q000382:microbiology; D003470:Culture Media; D004531:Eggs / Q000382:microbiology; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007202:Indicators and Reagents; D008460:Meat / Q000382:microbiology; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012475:Salmonella / Q000737:chemistry*
chemical_list: D003470:Culture Media; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic