title: Hydrophilic interaction chromatography coupled to MS for metabonomic/metabolomic studies.
abstract: Hydrophilic interaction chromatography (HILIC) is a relatively recently introduced mode of liquid-phase separations. Recently, HILIC has been used for coupling to MS in metabonomic/metabolomic studies to provide a complementary tool to the widely used reversed-phase (RP) chromatographic separations. The combination of HILIC with MS detection covers a number of polar metabolites that are typically nonretained in RPLC-mode separations and thus enlarging the number of detected analytes. This way of metabolite profiling thus provides more comprehensive metabolome coverage than using RP chromatography alone. This review describes the applications and the utility of HILIC-MS in metabolomic/metabonomic studies and highlights certain characteristic examples in the life and plant-food sciences.
authors.affiliation: Laboratory of Forensic Medicine and Toxicology, Medical School, Aristotle University of Thessaloniki, Thessaloniki Greece.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Spagou
authors.forename: Helen
authors.initials: H
authors.lastname: Tsoukali
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Raikos
authors.forename: Helen
authors.initials: H
authors.lastname: Gika
authors.forename: Ian D
authors.initials: ID
authors.lastname: Wilson
authors.forename: Georgios
authors.initials: G
authors.lastname: Theodoridis
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics