title: Cytotoxic and cell cycle effects induced by two herbal extracts on human cervix carcinoma and human breast cancer cell lines.
abstract: In recent times interest has increased in the complementary medicine of cancer patients. Two herbal mixtures were prepared from 17 and 12 plants, respectively. The goal of this study was to examine the in vitro cytotoxic and cell cycle effects of the aqueous-ethanol extracts (Extract 1 and Extract 2) obtained by maceration of the mixtures. The two extracts investigated exhibited significant antiproliferative activity toward two human breast cancer cell lines (MDA-MB-361 and MDA-MB-453) and a human cervix carcinoma cell line (HeLa) with 50% inhibitory concentration (IC(50)) values ranging from 9.92 to 17.38 microL/mL. The extracts did not exert any significant cytotoxicity toward healthy human peripheral blood mononuclear cells. In vitro antitumor activities were accompanied by an important apoptotic fraction of all cell lines after treatment with the extracts. The amount of total phenols was similar in both extracts, whereas the concentration of total tannins was significantly higher in Extract 1. Extract 1 was also found to be a stronger free radical scavenger, with an IC(50) value of 13.4 microg/mL. Both extracts contained rosmarinic acid, while ursolic acid was identified in Extract 2.
authors.affiliation: Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia.
authors.forename: Tatjana P
authors.initials: TP
authors.lastname: Stanojković
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Konić-Ristić
authors.forename: Zorica D
authors.initials: ZD
authors.lastname: Juranić
authors.forename: Katarina
authors.initials: K
authors.lastname: Savikin
authors.forename: Gordana
authors.initials: G
authors.lastname: Zdunić
authors.forename: Nebojsa
authors.initials: N
authors.lastname: Menković
authors.forename: Milka
authors.initials: M
authors.lastname: Jadranin
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002934:Cinnamates / Q000032:analysis / Q000494:pharmacology; D053630:Depsides / Q000032:analysis / Q000494:pharmacology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D019684:Magnoliopsida / Q000737:chemistry; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D013634:Tannins / Q000032:analysis / Q000494:pharmacology; D014315:Triterpenes / Q000032:analysis / Q000494:pharmacology; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy*; D000097123:Rosmarinic Acid; D000097245:Ursolic Acid
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D002934:Cinnamates; D053630:Depsides; D010636:Phenols; D010936:Plant Extracts; D013634:Tannins; D014315:Triterpenes; D000097123:Rosmarinic Acid; D000097245:Ursolic Acid