title: Effects of GH gene polymorphism and sex on carcass traits and fatty acid compositions in Japanese Black cattle.
abstract: To investigate the effects of bovine growth hormone (bGH) gene polymorphism on carcass traits and fatty acid compositions in Japanese Black cattle caused by nucleotide substitution of CTG (allele A)/GTG (allele B) at codon 127 and of ACG (allele A and B)/ATG (allele C) at codon 172 of bGH, GH genotypes of 135 cattle were determined using allele specific-multiplex polymerase chain reaction (PCR). Allele A gave greater rib thickness and lower melting point of fat (MP) while allele B gave higher C18:1% (P < 0.05). Allele C gave higher C18:1, monounsaturated fatty acid (MUFA), unsaturated fatty acid (USFA) percentages (P < 0.05). It also gave lower saturated fatty acid (SFA) percentages, higher MUFA/SFA and USFA/SFA ratios, and lower MP (P < 0.05). Interactions of sex and GH alleles were analyzed. In heifers, allele A gave higher carcass weight, daily carcass gain, rib eye area, rib thickness, subcutaneous fat thickness, and BMS while allele B gave greater rib eye area and rib thickness (P < 0.05). Allele C gave higher C18:1 (P < 0.01), MUFA (P < 0.01), USFA percentages (P < 0.05) and MUFA/SFA and USFA/SFA ratios (P < 0.01), and lower C16:0 and SFA percentages (P < 0.05) and MP (P < 0.01). GH gene polymorphism affected carcass traits and fatty acid compositions although the effects were more pronounced in heifers.
authors.affiliation: Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Aoba, Sendai, Japan. astrid@bios.tohoku.ac.jp
authors.forename: Astrid
authors.initials: A
authors.lastname: Ardiyanti
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Oki
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Suda
authors.forename: Keiichi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Koichi
authors.initials: K
authors.lastname: Chikuni
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Obara
authors.forename: Kazuo
authors.initials: K
authors.lastname: Katoh
mesh_terms: D000483:Alleles; D000822:Animal Husbandry; D000818:Animals; D002102:Cadaver; D002417:Cattle; D005229:Fatty Acids, Monounsaturated / Q000032:analysis*; D005260:Female; D005838:Genotype; D013006:Growth Hormone / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D012727:Sex Characteristics; D012737:Sex Factors; D050151:Subcutaneous Fat / Q000737:chemistry / Q000378:metabolism*; D044366:Transition Temperature
chemical_list: D005229:Fatty Acids, Monounsaturated; C073294:growth hormone, bovine; D013006:Growth Hormone