title: Osteoblast response to polymethyl methacrylate bioactive glass composite.
abstract: Polymethylmethacrylate (PMMA) has been used in many orthopedic and dental applications since the 1960s. Biocompatibility of newly developed surface porous fiber reinforced (SPFR) PMMA based composite has not been previously proven in cell culture environment. Analysis of rat bone marrow stromal cells grown on the different test materials showed only little difference in normalized cell activity or bone sialoprotein (BSP) production between the test materials, but the osteocalcin (OC) levels remained higher (P < 0.015-0.005) through out the test with SPFR-material when compared to tissue culture poly styrene (TCPS). The cells grown on SP-FRC material also showed highest calcium depletion from the culture medium (P < 0.026-0.001) when compared to all other test substrates. SEM images of the cultured samples confirmed that all the materials enabled cell spreading and growth on their surface, but the roughened surface remarkably enhanced this process of cell attachment, division and calcified nodule formation. This study shows that the SP-FRC composite material does not elicit harmful/toxic reactions in cell cultures more than neutral TCPS and can be considered biocompatible. The material possesses good capabilities to form new mineralized tissue onto its surface, and through that a possibility to bond directly to bone. Rough surface seems to enhance osteoblast proliferation and formation of mineralized extracellular matrix.
authors.affiliation: Department of Orthopedics and Traumatology, University Hospital of Turku, University of Turku, Lemminkäisenkatu 2, 20520, Turku, Finland. mikko.hautamaki@fimnet.fi
authors.forename: M
authors.initials: M
authors.lastname: Hautamäki
authors.forename: V V
authors.initials: VV
authors.lastname: Meretoja
authors.forename: R H
authors.initials: RH
authors.lastname: Mattila
authors.forename: A J
authors.initials: AJ
authors.lastname: Aho
authors.forename: P K
authors.initials: PK
authors.lastname: Vallittu
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism; D005898:Glass / Q000737:chemistry; D058574:Integrin-Binding Sialoprotein; D008297:Male; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D015675:Osteocalcin / Q000378:metabolism; D019904:Polymethyl Methacrylate / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins
chemical_list: C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D012795:Sialoglycoproteins; D015675:Osteocalcin; D019904:Polymethyl Methacrylate