title: Derivation of Man-1 and Man-2 research grade human embryonic stem cell lines.
abstract: We report here the derivation of two new human embryonic stem cell lines, Man-1 and Man-2, and their full characterization as novel pluripotent stem cell lines. Man-1 was derived from an embryo surplus to requirement from routine IVF, while Man-2 was obtained from an oocyte classified as failed to fertilise and subsequently chemically activated. We report the characterisation of pluripotency and the differentiation potential of these lines. Work is in progress to establish novel methods of stem cell derivation and culture, which will avoid the use of xenobiotics and be relevant to clinical production of human embryonic stem cell lines. Both newly derived human embryonic stem cell lines will be available for the research community from the UK Stem Cell Bank (http://www.ukstemcellbank.org.uk).
authors.affiliation: North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, Manchester M13 3PT, UK. mavi.camarasa@caubet-cimera.es
authors.forename: Maria V
authors.initials: MV
authors.lastname: Camarasa
authors.forename: Robbie W
authors.initials: RW
authors.lastname: Kerr
authors.forename: Sharon F
authors.initials: SF
authors.lastname: Sneddon
authors.forename: Nicola
authors.initials: N
authors.lastname: Bates
authors.forename: Lisa
authors.initials: L
authors.lastname: Shaw
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Oldershaw
authors.forename: Fiona
authors.initials: F
authors.lastname: Small
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Baxter
authors.forename: Tristan R
authors.initials: TR
authors.lastname: Mckay
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Brison
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Kimber
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line / Q000166:cytology*; D002871:Chromosome Banding; D003470:Culture Media / Q000494:pharmacology; D033041:Embryo Research; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice
chemical_list: D015415:Biomarkers; D003470:Culture Media