title: Increased phosphorylation of vimentin in noninfiltrative meningiomas.
abstract: BACKGROUND
  Tissue invasion or tissue infiltration are clinical behaviors of a poor-prognosis subset of meningiomas. We carried out proteomic analyses of tissue extracts to discover new markers to accurately distinguish between infiltrative and noninfiltrative meningiomas.
  
  
  RESULTS
  Protein lysates of 64 different tissue samples (including two brain-invasive and 32 infiltrative tumors) were submitted to SELDI-TOF mass spectrometric analysis. Mass profiles were used to build up both unsupervised and supervised hierarchical clustering. One marker was found at high levels in noninvasive and noninfiltrative tumors and appeared to be a discriminative marker for clustering infiltrative and/or invasive meningiomas versus noninvasive meningiomas in two distinct subsets. Sensitivity and specificity were 86.7% and 100%, respectively. This marker was purified and identified as a multiphosphorylated form of vimentin, a cytoskeletal protein expressed in meningiomas.
  
  
  CONCLUSIONS
  Specific forms of vimentin can be surrogate molecular indicators of the invasive/infiltrative phenotype in tumors.
authors.affiliation: Grenoble Institut des Neurosciences, INSERM U836, Université Joseph Fourier, Grenoble, France.
authors.forename: Ali
authors.initials: A
authors.lastname: Bouamrani
authors.forename: Claire
authors.initials: C
authors.lastname: Ramus
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Gay
authors.forename: Laurent
authors.initials: L
authors.lastname: Pelletier
authors.forename: Myriam
authors.initials: M
authors.lastname: Cubizolles
authors.forename: Sabine
authors.initials: S
authors.lastname: Brugière
authors.forename: Didier
authors.initials: D
authors.lastname: Wion
authors.forename: François
authors.initials: F
authors.lastname: Berger
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Issartel
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000378:metabolism*; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000145:classification / Q000175:diagnosis / Q000378:metabolism*; D008579:Meningioma / Q000145:classification / Q000175:diagnosis / Q000378:metabolism*; D008875:Middle Aged; D008970:Molecular Weight; D010766:Phosphorylation; D040901:Proteomics / Q000379:methods; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014746:Vimentin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D014746:Vimentin