title: Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14.
abstract: The prolonged effects of neonatal SRIH-14 treatment on pituitary ACTH cells were investigated. Neonatal female rats were injected subcutaneously with SRIH (20 microg/100g b.w.) every 12 hours for five consecutive days (3rd-7th day of life). Groups of rats were then killed at the juvenile (16th day), peripubertal (38th day) or adult (80th day) stage. ACTH cells were visualized using the peroxidase-antiperoxidase immunocytochemical procedure. Morphometry and stereology were used to evaluate the ACTH-immunoreactive cell volume and volume density. The histological and immunocytochemical characteristics of ACTH cells in neonatally treated females were changed in all examined periods. Thus, SRIH-14 induced significant (p<0.05) decreases of ACTH cell volume in juvenile, peripubertal and adult rats by 26%, 39% and 14%, respectively, in comparison to the corresponding controls. The volume density of ACTH cells was also diminished (by 31%; p<0.05) at the juvenile stage in comparison with the corresponding controls. In peripubertal and adult rats, the volume densities of ACTH cells were somewhat lower (17% and 14%, respectively), but the decreases did not reach statistical significance. These findings suggest that neonatal treatment with SRIH-14 exerts a marked prolonged inhibitory effect on ACTH cell morphology extending to the adult period of life.
authors.affiliation: Institute for Biological Research, Sinisa Stanković, University of Belgrade, Belgrade, Serbia.
authors.forename: Verica
authors.initials: V
authors.lastname: Milosević
authors.forename: Natasa
authors.initials: N
authors.lastname: Nestorović
authors.forename: Milica
authors.initials: M
authors.lastname: Terzić
authors.forename: Natasa
authors.initials: N
authors.lastname: Ristić
authors.forename: Vladimir
authors.initials: V
authors.lastname: Ajdzanović
authors.forename: Svetlana
authors.initials: S
authors.lastname: Trifunović
authors.forename: Milka
authors.initials: M
authors.lastname: Sekulić
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D004305:Dose-Response Relationship, Drug; D005260:Female; D007150:Immunohistochemistry; D009929:Organ Size / Q000187:drug effects; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000494:pharmacology*
chemical_list: D013004:Somatostatin; D000324:Adrenocorticotropic Hormone