title: Beta2-adrenergic receptor redistribution in heart failure changes cAMP compartmentation.
abstract: The beta1- and beta2-adrenergic receptors (betaARs) on the surface of cardiomyocytes mediate distinct effects on cardiac function and the development of heart failure by regulating production of the second messenger cyclic adenosine monophosphate (cAMP). The spatial localization in cardiomyocytes of these betaARs, which are coupled to heterotrimeric guanine nucleotide-binding proteins (G proteins), and the functional implications of their localization have been unclear. We combined nanoscale live-cell scanning ion conductance and fluorescence resonance energy transfer microscopy techniques and found that, in cardiomyocytes from healthy adult rats and mice, spatially confined beta2AR-induced cAMP signals are localized exclusively to the deep transverse tubules, whereas functional beta1ARs are distributed across the entire cell surface. In cardiomyocytes derived from a rat model of chronic heart failure, beta2ARs were redistributed from the transverse tubules to the cell crest, which led to diffuse receptor-mediated cAMP signaling. Thus, the redistribution of beta(2)ARs in heart failure changes compartmentation of cAMP and might contribute to the failing myocardial phenotype.
authors.affiliation: Department of Cardiac Medicine, National Heart and Lung Institute, Imperial College London, Dovehouse Street, London SW3 6LY, UK.
authors.forename: Viacheslav O
authors.initials: VO
authors.lastname: Nikolaev
authors.forename: Alexey
authors.initials: A
authors.lastname: Moshkov
authors.forename: Alexander R
authors.initials: AR
authors.lastname: Lyon
authors.forename: Michele
authors.initials: M
authors.lastname: Miragoli
authors.forename: Pavel
authors.initials: P
authors.lastname: Novak
authors.forename: Helen
authors.initials: H
authors.lastname: Paur
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Lohse
authors.forename: Yuri E
authors.initials: YE
authors.lastname: Korchev
authors.forename: Sian E
authors.initials: SE
authors.lastname: Harding
authors.forename: Julia
authors.initials: J
authors.lastname: Gorelik
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002908:Chronic Disease; D000242:Cyclic AMP / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D006333:Heart Failure / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008853:Microscopy / Q000379:methods; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D018342:Receptors, Adrenergic, beta-1 / Q000235:genetics / Q000378:metabolism; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics / Q000378:metabolism*; D012508:Sarcolemma / Q000378:metabolism* / Q000648:ultrastructure; D015398:Signal Transduction
chemical_list: D018342:Receptors, Adrenergic, beta-1; D018343:Receptors, Adrenergic, beta-2; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases