title: Physical mapping of rDNA genes corroborates allopolyploid origin in apomictic Brachiaria brizantha.
abstract: Brachiaria (Trin.) Griseb belongs to the family Poaceae, and within the genus, apomixis or sexuality is present in different accessions of the same species. The majority of Brachiaria species are polyploid and apomictic, making strategies for crop improvement by breeding very intricate. In spite of the high frequency of apomictic polyploids, the relationship of polyploidy and hybridization with apomixis in Brachiaria is still unclear. Further analysis requires detailed knowledge regarding the genomic composition of the polyploids. The present work introduces the use of fluorescent in situ hybridization (FISH) into cytogenetic analysis of Brachiaria. Physical mapping of heterologous rDNA sequences, associated with conventional karyotyping of the B. brizantha diploid sexual (BRA 002747) and the tetraploid apomictic (BRA000591) accessions, provided evidence of the latter being of allopolyploid origin. Based on our results and on previous knowledge on apomixis in B. brizantha, we suggest that the origin of apomixis was probably a consequence of hybridization.
authors.affiliation: Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica (PqEB), Final W5 Norte, P.O.Box 02354, Brasília, DF, 70770-900, Brazil.
authors.forename: Stephan
authors.initials: S
authors.lastname: Nielen
authors.forename: Lucas M
authors.initials: LM
authors.lastname: Almeida
authors.forename: Vera T C
authors.initials: VT
authors.lastname: Carneiro
authors.forename: Ana Claudia G
authors.initials: AC
authors.lastname: Araujo
mesh_terms: D035201:Brachiaria / Q000235:genetics*; D001947:Breeding; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D006824:Hybridization, Genetic; D011123:Polyploidy
chemical_list: D004275:DNA, Ribosomal