Livin gene plays a role in drug resistance of colon cancer cells.
OBJECTIVE
The objective of this study was to investigate the effect of knockdown of Livin expression on reversing drug resistance phenotype of colon cancer HCT-8/V cells.


METHODS
Specific short hairpin RNA (shRNA) was chosen and transfected in human colon cancer HCT-8/V cell line. Cell apoptosis and chemosensitivity were evaluated following downregulation of Livin expression.


RESULTS
In the current study, Livin was found to be highly expressed in the HCT-8/V colon cancer cells, which were resistant to several anti-tumor drugs. Knocking down of Livin expression in HCT-8/V cells by specific RNAi facilitated the apoptosis of HCT-8/V cells in response to vincristine (VCR), etoposide (VP-16), and 5-flourouracil (5-FU). Chemosensitivity assay confirmed the results and demonstrated the reversal of drug resistance phenotype of HCT-8/V cells.


CONCLUSIONS
These data suggest that specific silencing of Livin gene expression could be a promising target for further research in clinical chemotherapy of colon cancer.
Wang
Xudong
X
Department of Laboratory Medicine Center, Affiliated Hospital of Nantong University, Jiangsu, PR China.
Xu
Jian
J
Ju
Shaoqing
S
Ni
Hongbing
H
Zhu
Jianhua
J
Wang
Huimin
H
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005047:Etoposide / Q000494:pharmacology / Q000627:therapeutic use; D005472:Fluorouracil / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism*; D034622:RNA Interference / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014750:Vincristine / Q000494:pharmacology / Q000627:therapeutic use
D048868:Adaptor Proteins, Signal Transducing; D000970:Antineoplastic Agents; C419866:BIRC7 protein, human; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D014750:Vincristine; D005047:Etoposide; D005472:Fluorouracil