Prospective controlled animal study on biopsy sampling with new flexible cryoprobes versus forceps: evaluation of biopsy size, histological quality and bleeding risk.
BACKGROUND
Cryoextraction is a procedure used for the recanalization of obstructed airways caused by visible exophytic endobronchial tumor. Biopsy samples obtained by this technique have been shown to be useful for histological assessment.


OBJECTIVE
The aim of the present animal study was to systematically evaluate biopsy size, histological quality and bleeding risk after cryobiopsy with new, flexible cryoprobes in comparison with forceps biopsy, serving as the gold standard.


METHODS
Biopsies were obtained from anesthetized pigs with the flexible bronchoscopy technique, and evaluated histologically with respect to their size and quality. Bleeding frequency, bleeding duration and histological changes in the biopsy bed were also recorded.


RESULTS
Cryobiopsies were significantly larger than forceps biopsies. The size of cryobiopsies was dependent on the freezing time. The histological quality of the cryobiopsy specimenswas not impaired by the freezing process, whereas forceps biopsies showed typical crush artifacts. Despite the larger defects left in the tracheobronchial system after cryobiopsy, bleeding frequency and duration were not higher compared to forceps biopsy.


CONCLUSIONS
Since cryobiopsy sampling is not associated with a higher bleeding risk compared with forceps biopsy, this new biopsy technique offers--in addition to a good specimen quality--a safe and valuable tool with the potential of improving the outcome of diagnostic endoscopy.
Franke
Karl-Josef
KJ
Department of Pneumology and Critical Care Medicine, Helios Klinik Ambrock, University of Witten/Herdecke, Hagen, Germany. karl-josef.franke@helios-kliniken.de
Theegarten
Dirk
D
Hann von Weyhern
Claus
C
Nilius
Georg
G
Brueckner
Christiane
C
Hetzel
Jürgen
J
Hetzel
Martin
M
Ruhle
Karl-Heinz
KH
Enderle
Markus D
MD
Szyrach
Mara N I
MN
D000818:Animals; D001706:Biopsy / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods; D016063:Blood Loss, Surgical / Q000706:statistics & numerical data; D001999:Bronchoscopy; D003452:Cryosurgery / Q000009:adverse effects / Q000379:methods*; D008168:Lung / Q000473:pathology*; D011446:Prospective Studies; D013552:Swine