Anti-aging effects of cyanidin under a stress-induced premature senescence cellular system.
The anti-aging effects of cyanidin were investigated under stress-induced premature senescence (SIPS) using WI-38 human diploid fibroblasts. WI-38 cells that were treated with 300 microM H(2)O(2) showed losses of cell viability, increased lipid peroxidation, and shortened cell lifespans. However, treatment with cyanidin attenuated cellular oxidative stress through increase of cell viability and the inhibition of lipid peroxidation. In addition, the life spans of young-, middle-, and old-aged WI-38 cells were prolonged by cyanidin treatment. Furthermore, H(2)O(2)-treated WI-38 cells significantly increased mRNA and protein expressions of nuclear factor-kappaB, cyclooxygenase-2, and inducible nitric oxide synthase, while those treated with cyanidin had significantly decreased expressions. These results suggest that cyanidin may delay the aging process by attenuating oxidative stress under the SIPS cellular model.
Choi
Mi Jin
MJ
Department of Food Science and Nutrition, Research Institute of Ecology for the Elderly, Pusan National University, Jangjeon-dong, Geumjeong-gu, Busan 609-735, Korea.
Kim
Boh Kyung
BK
Park
Kun Young
KY
Yokozawa
Takako
T
Song
Young Ok
YO
Cho
Eun Ju
EJ
D000872:Anthocyanins / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects*; D016922:Cellular Senescence / Q000187:drug effects* / Q000502:physiology; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide; D015227:Lipid Peroxidation / Q000187:drug effects; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism
D000872:Anthocyanins; D000975:Antioxidants; D016328:NF-kappa B; D010936:Plant Extracts; D012333:RNA, Messenger; C017154:cyanidin; D006861:Hydrogen Peroxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2