title: Study of blood porphyrin spectral profile for diagnosis of chronic renal failure.
abstract: The progression to end-stage renal failure is independent of the initial pathogenic mechanism. Metabolic acidosis is a common consequence of chronic renal failure that results from inadequate ammonium excretion and decreased tubular bicarbonate reabsorption. Protoporphyrin IX (PpIX) is the immediate metabolic precursor of the heme molecule. The purpose of this study was to evaluate the levels of erythrocytes protoporphyrin IX at an animal model during progressive renal disease. A total of 36 eight-week-old male Wistar rats were divided into six groups: Normal, 4 and 8 weeks after 5/6 nephrectomy (NX). Renal function was evaluated by creatinine clearance and plasma creatinine levels. The autofluorescence of erythrocytes porphyrin of healthy and NX rats was analyzed using fluorescence spectroscopy. Emission spectra were obtained by exciting the samples at 405 nm. Significant differences between normal and NX rats autofluorescence shape occurred in the 600-700 nm spectral region. A correlation was observed between emission band intensity at 635 nm and progression of renal disease.
authors.affiliation: Disciplina Nefrologia, Departamento de Medicina, Universidade Federal de São Paulo-UNIFESP-EPM, São Paulo, Brazil.
authors.forename: Vivian Regina
authors.initials: VR
authors.lastname: Tristão
authors.forename: Fernando Felippe
authors.initials: FF
authors.lastname: de Carvalho
authors.forename: Cinthia Zanini
authors.initials: CZ
authors.lastname: Gomes
authors.forename: Adriana Regina
authors.initials: AR
authors.lastname: Miranda
authors.forename: Cíntia C
authors.initials: CC
authors.lastname: Vequi-Suplicy
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Lamy
authors.forename: Nestor
authors.initials: N
authors.lastname: Schor
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Bellini
mesh_terms: D000138:Acidosis / Q000097:blood / Q000473:pathology; D000818:Animals; D003404:Creatinine / Q000097:blood; D018450:Disease Progression; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000097:blood / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D009392:Nephrectomy; D011166:Porphyrins / Q000097:blood*; D011524:Protoporphyrins; D051381:Rats; D017208:Rats, Wistar
chemical_list: D011166:Porphyrins; D011524:Protoporphyrins; D003404:Creatinine; C028025:protoporphyrin IX