Overexpression of Chromatin Assembly Factor-1/p60 helps to predict the prognosis of melanoma patients.
BACKGROUND
Cutaneous melanoma (CM) is the most lethal form of skin malignancy, which registers a constant increase in incidence worldwide. The identification of molecular alteration(s) involved in its biological aggressiveness represents a major challenge for researchers, considering that existing therapies are ineffective to treat metastasizing cases. The epigenetic control of chromatin dynamics during DNA synthesis, replication, and repair is fundamental for the orderly progression of cell proliferation. The Chromatin Assembly Factor 1 (CAF-1) complex acts as a major regulator of this process; its intermediate (p60) subunit has been recently proposed as a novel proliferation and prognostic marker for several tumors. We aimed to establish if the evaluation of the expression of CAF-1/p60 in primary CM may help define the prevision of outcome of patients.


METHODS
Immunohistochemistry with anti-CAF-1/p60 was performed on paraffin-embedded tissue sections of 130 cases of primary CM retrieved from the archive files of the Department of Biomorphological and Functional Sciences, Section of Pathology, University "Federico II" of Naples, Italy. Results were compared with histopathological and follow-up data of patients.


RESULTS
CAF-1/p60 was expressed in all CM. A significant statistical association between the overexpression of the protein and the occurrence of skin, node and/or distant metastases (P < 0.05) emerged, independently from histopathological prognostic factors.


CONCLUSIONS
CAF-1/p60 looks promising as a new prognostic marker for CM and sheds new light on the molecular events associated with photocancerogenesis and melanoma biology.The screening for CAF-1/p60 might contribute to the molecular sub-classification of CM, with improved translational outcomes.
Mascolo
Massimo
M
Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples Federico II, School of Medicine, Naples, Italy.
Vecchione
Maria Luisa
ML
Ilardi
Gennaro
G
Scalvenzi
Massimiliano
M
Molea
Guido
G
Di Benedetto
Maria
M
Nugnes
Loredana
L
Siano
Maria
M
De Rosa
Gaetano
G
Staibano
Stefania
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D056504:Chromatin Assembly Factor-1 / Q000096:biosynthesis*; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008545:Melanoma / Q000473:pathology*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011379:Prognosis; D012878:Skin Neoplasms / Q000473:pathology*; D016896:Treatment Outcome
D056504:Chromatin Assembly Factor-1