[Comparative data regarding two HPLC methods for determination of isoniazid].
For the determination of isoniazide (isonicotinic acid hydrazide - HIN) two different HPLC methods were developed and validated.


METHODS
Both experiments were performed using a Waters 2695 liquid chromatograph and a UV - Waters 2489 detector. The first method (I) used a Nucleosil 100-10 C18 column (250 x 4.6 mm), a mobile phase formed by a mixture of acetonitrile/10(-2) M oxalic acid (80/20) and a flow of 1.5 mL/ min; detection was done at 230 nm. The second method (II) used a Luna 100-5 C18 column (250 x 4.6 mm), a mobile phase formed by a mixture of methanol/acetate buffer, pH = 5.0 (20/ 80), a flow of 1 mL/min; detection was done at 270 nm.


RESULTS
Both methods were validated, the correlation coefficients were 0.9998 (I) and 0.9999 (II), the detection limits were 0.6 microg/mL (I) and 0.055 microg/mL (II), the quantitation limits were 1.9 microg/mL (I) and 0.2 microg/ mL (II). There were also studied: the system precision (RSD = 0.1692% (I) and 0.2000% (II)), the method precision (RSD = 1.1844% (I) and 0.6170% (II)) and the intermediate precision (RSD = 1.8058% (I) and 0.5970% (II)). The accuracy was good, the calculated recoveries were 102.66% (I) and 101.36 (II).


CONCLUSIONS
Both validated methods were applied for HIN determination from tablets with good and comparable results.
Gârbuleţ
Daniela
D
Facultatea de Farmacie, Disciplina Chimie analitică, Universitatea de Medicină si Farmacie "Gr.T. Popa" Iaşi.
Spac
A F
AF
Dorneanu
V
V
D000995:Antitubercular Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007538:Isoniazid / Q000032:analysis*; D013607:Tablets / Q000032:analysis
D000995:Antitubercular Agents; D013607:Tablets; D007538:Isoniazid