title: Antiretroviral drug resistance mutations in the reverse transcriptase gene of HIV-1 isolates from Northern Indian patients: a follow-up study.
abstract: The viral reverse transcriptase gene was amplified from the plasma of 27 drug-naïve and five drug-experienced HIV patients in Northern India. Follow-up samples of naïve patients after antiretroviral therapy initiation were collected in six patients at 3 months and three patients at 6 months. Phylogenetic analysis revealed two new recombinant forms, CRF_CH and CRF_CK, and an accessory non-nucleoside reverse transcriptase inhibitor mutation E138A, not previously reported from India. The major DRMs found in two 6-month follow-up samples were absent in their baseline blood samples. This is the first follow-up study to determine anti-retroviral drug resistance mutations in Indian HIV patients.
authors.affiliation: Department of Biochemistry, All India Institute of Medical Sciences, Room no. 3002, Ansari Nagar, New Delhi, India. mitusree@gmail.com
authors.forename: Shubhasree
authors.initials: S
authors.lastname: Dutta Choudhury
authors.forename: Alok K
authors.initials: AK
authors.lastname: Chaudhury
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kalra
authors.forename: Raiees
authors.initials: R
authors.lastname: Andrabi
authors.forename: Naveet
authors.initials: N
authors.lastname: Wig
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Biswas
authors.forename: Manju
authors.initials: M
authors.lastname: Bala
authors.forename: Kalpana
authors.initials: K
authors.lastname: Luthra
mesh_terms: D000293:Adolescent; D000328:Adult; D019943:Amino Acid Substitution / Q000235:genetics; D044966:Anti-Retroviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016000:Cluster Analysis; D024882:Drug Resistance, Viral; D005260:Female; D005500:Follow-Up Studies; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology*; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D055815:Young Adult
chemical_list: D044966:Anti-Retroviral Agents; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase