title: Assembly and biological and immunological properties of Newcastle disease virus-like particles.
abstract: Virus-like particles (VLPs) released from avian cells expressing the Newcastle disease virus (NDV) strain AV proteins NP, M, HN (hemagglutinin-neuraminidase), and F were characterized. The VLP-associated HN and F glycoproteins directed the attachment of VLPs to cell surfaces and fusion of VLP membranes with red blood cell membranes, indicating that they were assembled into VLPs in an authentic conformation. These particles were quantitatively prepared and used as an immunogen, without adjuvant, in BALB/c mice. The resulting immune responses, detected by enzyme-linked immunosorbent assay (ELISA), virus neutralization, and intracellular cytokine staining, were comparable to the responses to equivalent amounts of inactivated NDV vaccine virus. HN and F proteins from another strain of NDV, strain B1, could be incorporated into these VLPs. Foreign peptides were incorporated into these VLPs when fused to the NP or HN protein. The ectodomain of a foreign glycoprotein, the Nipah virus G protein, fused to the NDV HN protein cytoplasmic and transmembrane domains was incorporated into ND VLPs. Thus, ND VLPs are a potential NDV vaccine candidate. They may also serve as a platform to construct vaccines for other pathogens.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA.
authors.forename: Lori W
authors.initials: LW
authors.lastname: McGinnes
authors.forename: Homer
authors.initials: H
authors.lastname: Pantua
authors.forename: Jason P
authors.initials: JP
authors.lastname: Laliberte
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Gravel
authors.forename: Surbhi
authors.initials: S
authors.lastname: Jain
authors.forename: Trudy G
authors.initials: TG
authors.lastname: Morrison
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D016207:Cytokines / Q000096:biosynthesis; D015707:HN Protein / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009522:Newcastle disease virus / Q000235:genetics* / Q000276:immunology*; D045405:Nipah Virus; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics / Q000378:metabolism; D022721:Vaccines, Virosome / Q000008:administration & dosage / Q000276:immunology; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000276:immunology* / Q000378:metabolism* / Q000472:pathogenicity; D022701:Virosomes / Q000276:immunology / Q000378:metabolism; D019065:Virus Assembly; D053585:Virus Attachment; D053586:Virus Internalization
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D016207:Cytokines; D015707:HN Protein; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D022721:Vaccines, Virosome; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D022701:Virosomes; C409209:nucleoprotein, Newcastle disease virus