Circulating tumor cells in metastatic colorectal cancer: efficacy and feasibility of different enrichment methods.
Comprehensive in vitro and in vivo studies comparing EpCAM-based methods with other cytometric CTC enrichment technologies in metastatic colorectal cancer (mCRC) patients are lacking. We compare four manual cytometric methods to detect CTCs in vitro and in mCRC patients. The EpCAM-based technology, MACS HEA MicroBeads((R)), showed a significant better tumor cell recovery rate compared to other cytometric methods (p-value<0.0001). CTCs of 38 mCRC patients were enriched with MACS HEA MicroBeads(R). Progression-free survival did significantly differ between mCRC patients without detectable and with >or= 1 CTCs (p=0.007). CTC enrichment with EpCAM coupled antibodies is superior to other cytometric methods and is a feasible method for CTC detection in mCRC patients.
Königsberg
Robert
R
Applied Cancer Research - Institution for Translational Research Vienna (ACR-ITR Vienna)/CEADDP, Vienna, Austria. robert.koenigsberg@gmx.at <robert.koenigsberg@gmx.at>
Gneist
Margit
M
Jahn-Kuch
Daniela
D
Pfeiler
Georg
G
Hager
Gudrun
G
Hudec
Marcus
M
Dittrich
Christian
C
Zeillinger
Robert
R
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods; D015179:Colorectal Neoplasms / Q000097:blood* / Q000473:pathology*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009360:Neoplastic Cells, Circulating / Q000473:pathology*