title: Simultaneous determination of seven main alkaloids of Chelidonium majus L. by ultra-performance LC with photodiode-array detection.
abstract: A simple and rapid method for the simultaneous determination of seven isoquinoline alkaloids, protopine, chelidonine, coptisine, stylopine, sanguinarine, berberine, and chelerythrine, in Chelidonium majus L. (Ch. majus) samples by ultra-performance LC method with photodiode array detection is described. The baseline separation of these compounds was performed with (A) acetonitrile-(B) ammonium acetate (10 mM, adjusted to pH 3.0 with acetic acid) as the mobile phase using a C18 RP column (2.1x100 mm, 1.7 microm). Optimized conditions resulted in excellent peak shapes. The seven alkaloids were completely separated within 20 min. Good linear behaviors (r > or = 0.9992) over the investigated concentration ranges were observed for all the analytes. Validation proved the repeatability of the method was good and recovery was satisfactory. The validated method was successfully applied for 20 batches of Ch. majus. These results demonstrated that the ultra-performance LC photodiode array method proposed was very useful in the analysis and quality control of Ch. majus.
authors.affiliation: Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Gu
authors.forename: Dawei
authors.initials: D
authors.lastname: Qian
authors.forename: Jin-ao
authors.initials: JA
authors.lastname: Duan
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Jianming
authors.initials: J
authors.lastname: Guo
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.forename: Sheng
authors.initials: S
authors.lastname: Guo
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D028281:Chelidonium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid
chemical_list: D000470:Alkaloids