title: A glial signal consisting of gliomedin and NrCAM clusters axonal Na+ channels during the formation of nodes of Ranvier.
abstract: Saltatory conduction requires high-density accumulation of Na(+) channels at the nodes of Ranvier. Nodal Na(+) channel clustering in the peripheral nervous system is regulated by myelinating Schwann cells through unknown mechanisms. During development, Na(+) channels are first clustered at heminodes that border each myelin segment, and later in the mature nodes that are formed by the fusion of two heminodes. Here, we show that initial clustering of Na(+) channels at heminodes requires glial NrCAM and gliomedin, as well as their axonal receptor neurofascin 186 (NF186). We further demonstrate that heminodal clustering coincides with a second, paranodal junction (PNJ)-dependent mechanism that allows Na(+) channels to accumulate at mature nodes by restricting their distribution between two growing myelin internodes. We propose that Schwann cells assemble the nodes of Ranvier by capturing Na(+) channels at heminodes and by constraining their distribution to the nodal gap. Together, these two cooperating mechanisms ensure fast and efficient conduction in myelinated nerves.
authors.affiliation: Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Feinberg
authors.forename: Yael
authors.initials: Y
authors.lastname: Eshed-Eisenbach
authors.forename: Shahar
authors.initials: S
authors.lastname: Frechter
authors.forename: Veronique
authors.initials: V
authors.lastname: Amor
authors.forename: Daniela
authors.initials: D
authors.lastname: Salomon
authors.forename: Helena
authors.initials: H
authors.lastname: Sabanay
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Dupree
authors.forename: Martin
authors.initials: M
authors.lastname: Grumet
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Brophy
authors.forename: Peter
authors.initials: P
authors.lastname: Shrager
authors.forename: Elior
authors.initials: E
authors.lastname: Peles
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D001369:Axons / Q000378:metabolism*; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004594:Electrophysiology; D005455:Fluorescent Antibody Technique; D051379:Mice; D018345:Mice, Knockout; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000378:metabolism; D009413:Nerve Fibers, Myelinated / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism; D009431:Neural Conduction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011901:Ranvier's Nodes / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000378:metabolism*; D015222:Sodium Channels / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D009414:Nerve Growth Factors; C504593:Nfasc protein, mouse; C491151:Nrcam protein, mouse; D012333:RNA, Messenger; D015222:Sodium Channels; C520020:gliomedin protein, mouse