Nanofiber micro-dispersed oxidized cellulose as a carrier for topical antimicrobials: first experience.
BACKGROUND
Micro-dispersed oxidized cellulose, already used for hemostasis, might be helpful for introduction of an antimicrobial drug.


OBJECTIVE
To examine the effect of topically applied gentamicin attached to a new biodegradable carrier formed by micro-dispersed oxidized cellulose in microfiber and nanofiber form for treatment of acute wound infection and to assess the influence of this carrier on healing.


METHODS
A model of a full-thickness infected dermal wound was created in 12 female domestic pigs. The effectiveness of topical gentamicin delivered with micro-dispersed oxidized cellulose carrier was tested in acute wound infections caused by Staphylococcus aureus, Pseudomonas aeruginosa, or Escherichia coli.


RESULTS
The effectiveness of nanofiber micro-dispersed oxidized cellulose with gentamicin was proved according to culture findings. When assessed macroscopically, 100% of wounds treated by the nanofiber product had no signs of local infection. When microfiber micro-dispersed oxidized cellulose was used, cultures demonstrated residual bacteria in 94.4% of treated incisions despite the absence of clinically recognized infection.


CONCLUSIONS
Micro-dispersed oxidized cellulose carrier with a sufficient concentration of an attached antibiotic appears to be effective for the treatment of full-thickness skin infections. The positive influence of the product on the healing of a dermal incision was shown, and a good hemostatic effect was confirmed.
Lochman
Petr
P
Department of Field Surgery, Faculty of Military Health Sciences, University of Defence, Trebesská St. 1575, Hradec Králové, Czech Republic. lochmpet@yahoo.com
Plodr
Michal
M
Páral
Jirí
J
Smejkal
Karel
K
D000287:Administration, Topical; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D001424:Bacterial Infections / Q000188:drug therapy*; D002483:Cellulose, Oxidized / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage*; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification; D005260:Female; D005839:Gentamicins / Q000627:therapeutic use*; D057139:Nanofibers / Q000008:administration & dosage*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification; D013552:Swine; D016896:Treatment Outcome; D014945:Wound Healing; D014946:Wound Infection / Q000188:drug therapy*
D000891:Anti-Infective Agents, Local; D002483:Cellulose, Oxidized; D004337:Drug Carriers; D005839:Gentamicins