title: Modulation of RNA metal binding by flanking bases: 15N NMR evaluation of GC, tandem GU, and tandem GA sites.
abstract: (15)N NMR chemical shift changes in the presence of Mg(H(2)O)(6)(2+), Zn(2+), Cd(2+), and Co(NH(3))(6)(3+) were used to probe the effect of flanking bases on metal binding sites in three different RNA motifs. We found that: for GC pairs, the presence of a flanking purine creates a site for the soft metals Zn(2+) and Cd(2+) only; a GG.UU motif selectively binds only Co(NH(3))(6)(3+), while a UG.GU motif binds none of these metals; a 3' guanosine flanking the adenosine of a sheared GA.AG pair creates an unusually strong binding site that precludes binding to the cross-strand stacked guanosines within the tandem pair.
authors.affiliation: Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhao
authors.forename: Qianwei
authors.initials: Q
authors.lastname: Han
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Gengcheng
authors.initials: G
authors.lastname: Yang
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Shallop
authors.forename: Jane
authors.initials: J
authors.lastname: Liu
authors.forename: Barbara L
authors.initials: BL
authors.lastname: Gaffney
authors.forename: Roger A
authors.initials: RA
authors.lastname: Jones
mesh_terms: D020029:Base Pairing; D001665:Binding Sites; D008670:Metals / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D008670:Metals; D012313:RNA