title: Antibody dependent enhancement of frog virus 3 infection.
abstract: BACKGROUND
  Viruses included in the family Iridoviridae are large, icosahedral, dsDNA viruses that are subdivided into 5 genera. Frog virus 3 (FV3) is the type species of the genus Ranavirus and the best studied iridovirus at the molecular level. Typically, antibodies directed against a virus act to neutralize the virus and limit infection. Antibody dependent enhancement occurs when viral antibodies enhance infectivity of the virus rather than neutralize it.
  
  
  RESULTS
  Here we show that anti-FV3 serum present at the time of FV3 infection enhances infectivity of the virus in two non-immune teleost cell lines. We found that antibody dependent enhancement of FV3 was dependent on the Fc portion of anti-FV3 antibodies but not related to complement. Furthermore, the presence of anti-FV3 serum during an FV3 infection in a non-immune mammalian cell line resulted in neutralization of the virus. Our results suggest that a cell surface receptor specific to teleost cell lines is responsible for the enhancement.
  
  
  CONCLUSIONS
  This report represents the first evidence of antibody dependent enhancement in iridoviruses. The data suggests that anti-FV3 serum can either neutralize or enhance viral infection and that enhancement is related to a novel antibody dependent enhancement pathway found in teleosts that is Fc dependent.
authors.affiliation: Department of Biology, Trent University, Peterborough, ON, Canada.
authors.forename: Heather E
authors.initials: HE
authors.lastname: Eaton
authors.forename: Emily
authors.initials: E
authors.lastname: Penny
authors.forename: Craig R
authors.initials: CR
authors.lastname: Brunetti
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000378:metabolism*; D019067:Antibody-Dependent Enhancement; D002460:Cell Line; D002522:Chlorocebus aethiops; D043344:Chordata; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism; D017995:Ranavirus / Q000502:physiology*; D053586:Virus Internalization
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007141:Immunoglobulin Fc Fragments