[Investigations of the morphofunctional properties of red blood in human subjects during 9-day exposure in oxyargon medium with various oxygen content].
Biochemical investigations of red blood and oxygen transportation by erythrocyte hemoglobin were performed in volunteered for chamber experiments (0.14 MPa) with normal and hypoxic oxyargon medium (O2-Ar). Erythrocyte metabolism, lipid and phospholipids spectra, and effectiveness of oxygen release and retention by hemoglobin were studied in the baseline data collection period, on day 6 in hyperbaric and normoxic O2-Ar medium (13.7% O2), on day 3 in hyperbaric and hypoxic O2-Ar (9.9% O2) and on day 1 and 10 during the post-experiment rehab. Elevated pressure, hypoxia and ensuing decompression revealed an ATP reduction that could be a result of shifts on the membrane level and increased G6PD activity associated with cell reparation processes. Changes in membrane lipid and phospholipids composition point to an altered phase state of plasmatic membrane, i.e., increased viscosity which is indicative of possible impairment of membrane permeability As a rule, hyperbaria impacted oxygen transportation in blood of the subjects; effect of hypoxia combined with hyperbaria was not uniform ranging from none to decreasing or increasing oxygen transportation that can be extenuated by selectivity and individual sensitivity to the factors in the experiment Loss of O2 transportation efficiency by hemoglobin is most likely connected with the increase of plasmatic membrane viscosity that could affect hemoporphyrin conformation of membrane-bound hemoglobin and obstruct oxygen transport though membrane.
Ivanova
S M
SM
Morukov
B V
BV
Maksimov
G V
GV
Churin
A A
AA
Iarlykova
Iu V
IuV
Labetskaia
O I
OI
D000328:Adult; D001128:Argon / Q000008:administration & dosage*; D001692:Biological Transport / Q000502:physiology; D004338:Drug Combinations; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005500:Follow-Up Studies; D006454:Hemoglobins / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D000860:Hypoxia / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010100:Oxygen / Q000008:administration & dosage*; D013997:Time Factors
D004338:Drug Combinations; D006454:Hemoglobins; D001128:Argon; D010100:Oxygen