title: Percentage of regulatory T cells CD4+CD25+CD127- in HIV-infected patients is not reduced after cryopreservation.
abstract: Interest in regulatory T cells (Treg) has been continuously growing over the last 10years. In particular, a role for Treg has been recently reported in HIV. It is crucial to investigate their conservation over time, once sampled and purified. The objective of the present study was to investigate whether cryopreservation may modify recognition and measurement of relative frequency of Treg (CD4(+)CD25(+)CD127(-)) among PBMCs purified from 17 HIV-infected patients. Ours results indicate that cryopreservation does not affect this specific T lymphocyte subset.
authors.affiliation: Hospices Civils de Lyon, Hôpital E. Herriot, Pavillon E, 5 place d'Arsonval, 69437 LYON Cedex 03, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Venet
authors.forename: Christophe
authors.initials: C
authors.lastname: Malcus
authors.forename: Tristan
authors.initials: T
authors.lastname: Ferry
authors.forename: Françoise
authors.initials: F
authors.lastname: Poitevin
authors.forename: Guillaume
authors.initials: G
authors.lastname: Monneret
mesh_terms: D015704:CD4 Antigens / Q000097:blood / Q000276:immunology; D018791:CD4 Lymphocyte Count; D015331:Cohort Studies; D015925:Cryopreservation; D005260:Female; D015658:HIV Infections / Q000097:blood* / Q000276:immunology; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000097:blood / Q000276:immunology; D053699:Interleukin-7 Receptor alpha Subunit / Q000097:blood / Q000276:immunology; D008297:Male; D050378:T-Lymphocytes, Regulatory
chemical_list: D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit; D053699:Interleukin-7 Receptor alpha Subunit