title: Exploiting lipid-free tubing passive samplers and embryonic zebrafish to link site specific contaminant mixtures to biological responses.
abstract: The Biological Response Indicator Devices Gauging Environmental Stressors (BRIDGES) bio-analytical tool was developed in response to the need for a quantitative technology for assessing the toxicity of environmentally relevant contaminant mixtures. This tool combines passive samplers with the embryonic zebrafish model. When applied in an urban river it effectively linked site specific, bioavailable contaminant mixtures to multiple biological responses. Embryonic zebrafish exposed to extracts from lipid-free passive samplers that were deployed at five locations, within and outside of the Portland Harbor Superfund Megasite, displayed different responses. Six of the eighteen biological responses observed in 941 exposed zebrafish were significantly different between sites. This demonstrates the sensitivity of the bio-analytical tool for detecting spatially distinct toxicity in aquatic systems; bridging environmental exposure to biological response.
authors.affiliation: Environmental and Molecular Toxicology Department, Oregon State University, ALS 1007, Corvallis, OR 97331, USA.
authors.forename: Wendy E
authors.initials: WE
authors.lastname: Hillwalker
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Allan
authors.forename: Robert L
authors.initials: RL
authors.lastname: Tanguay
authors.forename: Kim A
authors.initials: KA
authors.lastname: Anderson
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects; D047108:Embryonic Development; D004784:Environmental Monitoring; D008055:Lipids / Q000737:chemistry; D045483:Rivers / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology*
chemical_list: D008055:Lipids; D014874:Water Pollutants, Chemical