Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisation.
The comet assay is widely used to measure DNA damage and repair in basic research, genotoxicity testing and human biomonitoring. The conventional format has 1 or 2 gels on a microscope slide, 1 sample per slide. To increase throughput, we have designed and tested a system with 12 smaller gels on one slide, allowing incubation of individual gels with different reagents or enzymes. Thus several times more samples can be analysed with one electrophoresis run, and fewer cells and smaller volumes of test solutions are required. Applications of the modified method include treatment with genotoxic agents at different concentrations; simultaneous analysis of different lesions using a range of enzymes; analysis of cell extracts for DNA repair activity; and fluorescent in situ hybridisation (FISH) to comet DNA with specific labelled probes.
Shaposhnikov
Sergey
S
Department of Nutrition, University of Oslo, Blindern, Oslo, Norway. serguc@usit.uio.no
Azqueta
Amaya
A
Henriksson
Sara
S
Meier
Silja
S
Gaivão
Isabel
I
Huskisson
Neville H
NH
Smart
Andrew
A
Brunborg
Gunnar
G
Nilsson
Mats
M
Collins
Andrew R
AR
D020552:Comet Assay / Q000295:instrumentation* / Q000379:methods*; D004249:DNA Damage; D006367:HeLa Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology; D011759:Pyrrolidines / Q000494:pharmacology; D011807:Quinolizines / Q000494:pharmacology
D017319:Photosensitizing Agents; D011759:Pyrrolidines; D011807:Quinolizines; C076809:Ro 19-8022