Female-specific flightless phenotype for mosquito control.
Dengue and dengue hemorrhagic fever are increasing public health problems with an estimated 50-100 million new infections each year. Aedes aegypti is the major vector of dengue viruses in its range and control of this mosquito would reduce significantly human morbidity and mortality. Present mosquito control methods are not sufficiently effective and new approaches are needed urgently. A "sterile-male-release" strategy based on the release of mosquitoes carrying a conditional dominant lethal gene is an attractive new control methodology. Transgenic strains of Aedes aegypti were engineered to have a repressible female-specific flightless phenotype using either two separate transgenes or a single transgene, based on the use of a female-specific indirect flight muscle promoter from the Aedes aegypti Actin-4 gene. These strains eliminate the need for sterilization by irradiation, permit male-only release ("genetic sexing"), and enable the release of eggs instead of adults. Furthermore, these strains are expected to facilitate area-wide control or elimination of dengue if adopted as part of an integrated pest management strategy.
Fu
Guoliang
G
Oxitec Limited, 71 Milton Park, Oxford OX14 4RX, United Kingdom.
Lees
Rosemary S
RS
Nimmo
Derric
D
Aw
Diane
D
Jin
Li
L
Gray
Pam
P
Berendonk
Thomas U
TU
White-Cooper
Helen
H
Scaife
Sarah
S
Kim Phuc
Hoang
H
Marinotti
Osvaldo
O
Jasinskiene
Nijole
N
James
Anthony A
AA
Alphey
Luke
L
D000199:Actins / Q000235:genetics; D000330:Aedes / Q000235:genetics* / Q000821:virology; D000818:Animals; D030801:Animals, Genetically Modified; D003433:Crosses, Genetic; D003716:Dengue Virus / Q000254:growth & development; D005260:Female; D005426:Flight, Animal; D006801:Humans; D019476:Insect Proteins / Q000235:genetics; D007303:Insect Vectors / Q000235:genetics* / Q000821:virology; D008297:Male; D009032:Mosquito Control / Q000379:methods*; D010641:Phenotype; D011401:Promoter Regions, Genetic / Q000235:genetics; D019595:Severe Dengue / Q000517:prevention & control / Q000635:transmission
D000199:Actins; D019476:Insect Proteins