title: Review of microarray studies for host-intracellular pathogen interactions.
abstract: Obligate intracellular bacteria are privileged soldiers on the battlefield that represent host-pathogen interactions. Microarrays are a powerful technology that can increase our knowledge about how bacteria respond to and interact with their hosts. This review summarizes the limitations inherent to host-pathogen interaction studies and essential strategies to improve microarray investigations of intracellular bacteria. We have compiled the comparative genomic and gene expression analyses of obligate intracellular bacteria currently available from microarrays. In this review we explore ways in which microarrays can be used to identify polymorphisms in different obligate intracellular bacteria such as Coxiella burnetii, Chlamydia trachomatis, Ehrlichia chaffeensis, Rickettsia prowazekii and Tropheryma whipplei. These microarray studies reveal that, while genomic content is highly conserved in obligate intracellular bacteria, genetic polymorphisms can potentially occur to increase bacterial pathogenesis. Additionally, changes in the gene expression of C. trachomatis throughout its life cycle, as well as changes in the gene expression profile of the pathogens R. prowazekii, Rickettsia rickettsii, Rickettsia typhi, T. whipplei and C. trachomatis following environmental changes, are discussed. Finally, an in vivo model of Rickettsia conorii within the skin is discussed. The gene expression analyses highlight the capacity of obligate intracellular bacteria to adapt to environmental changes and potentially to thwart the host response.
authors.affiliation: Université de la Méditerranée, URMITE IRD-CNRS 6236, Faculté de Médecine, 27 Bd Jean Moulin, 13385 Marseille cedex 05, France.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Leroy
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000472:pathogenicity*; D003600:Cytosol / Q000382:microbiology; D054884:Host-Pathogen Interactions; D020411:Oligonucleotide Array Sequence Analysis; D011110:Polymorphism, Genetic; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors