title: Immunological effect of subunit influenza vaccine entrapped by liposomes.
abstract: OBJECTIVE
  To elevate the immunological effect of subunit influenza vaccine in infants and aged people (over 60) using liposomal adjuvant in the context of its relatively low immunity and to investigate the relation between vaccine antigens and liposomal characteristics.
  
  
  METHODS
  Several formulations of liposomal subunit influenza vaccine were prepared. Their relevant characteristics were investigated to optimize the preparation method. Antisera obtained from immunizinged mice were used to evaluate the antibody titers of various samples by HI and ELISA.
  
  
  RESULTS
  Liposomal trivalent influenza vaccine prepared by film evaporation in combinedation with freeze-drying significantly increased its immunological effect in SPF Balb/c mice. Liposomal vaccine stimulated the antibody titer of H3N2, H1N1, and B much stronger than conventional influenza vaccine. As a result, liposomal vaccine (mean size: 4.5-5.5 microm, entrapment efficiency: 30%-40%) significantly increased the immunological effect of subunit influenza vaccine.
  
  
  CONCLUSIONS
  The immune effect of liposomal vaccine depends on different antigens, and enhanced immunity is not positively correlated with the mean size of liposome or its entrapped efficiency.
authors.affiliation: College of Life Sciences and Technology, Shanghai Jiao Tong University, Shanghai 200240, China. ashui10@126.com
authors.forename: Shui-Hua
authors.initials: SH
authors.lastname: Zhang
authors.forename: Jia-Xu
authors.initials: JX
authors.lastname: Liang
authors.forename: Shu-Yan
authors.initials: SY
authors.lastname: Dai
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Qiu
authors.forename: Yan-Rong
authors.initials: YR
authors.lastname: Yia
authors.forename: Yun
authors.initials: Y
authors.lastname: Pan
mesh_terms: D000818:Animals; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology; D009981:Influenza B virus / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D013047:Specific Pathogen-Free Organisms; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
chemical_list: D007252:Influenza Vaccines; D008081:Liposomes; D022223:Vaccines, Subunit