title: Generation of Merkel cell polyomavirus (MCV)-like particles and their application to detection of MCV antibodies.
abstract: The genome of a new human polyomavirus, known as Merkel cell polyomavirus (MCV), has recently been reported to be integrated within the cellular DNA of Merkel cell carcinoma (MCC), a rare human skin cancer. To investigate MCV seroprevalence in the general population, we expressed three different MCV VP1 in insect cells using recombinant baculoviruses. Viruslike particles (VLPs) were obtained with only one of the three VP1 genes. High-titer antibodies against VP1 VLPs were detected in mice immunized with MCV VLPs, and limited cross-reactivity was observed with BK polyomavirus (BKV) and lymphotropic polyomavirus (LPV). MCV antibodies were detected in 77% of the general population, with no variations according to age.
authors.affiliation: INSERM U618, Faculté de Pharmacie Ph Maupas, Université François Rabelais, and Service d'Anatomie et Cytologie Pathologiques, Hôpital Trousseau, CHRU de Tours, 31 Avenue Monge, 37200 Tours, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Touzé
authors.forename: Julien
authors.initials: J
authors.lastname: Gaitan
authors.forename: Françoise
authors.initials: F
authors.lastname: Arnold
authors.forename: Raphaël
authors.initials: R
authors.lastname: Cazal
authors.forename: Maxime J
authors.initials: MJ
authors.lastname: Fleury
authors.forename: Nicolas
authors.initials: N
authors.lastname: Combelas
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Sizaret
authors.forename: Serge
authors.initials: S
authors.lastname: Guyetant
authors.forename: Annabel
authors.initials: A
authors.lastname: Maruani
authors.forename: Marc
authors.initials: M
authors.lastname: Baay
authors.forename: Mauro
authors.initials: M
authors.lastname: Tognon
authors.forename: Pierre
authors.initials: P
authors.lastname: Coursaget
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics / Q000302:isolation & purification; D001739:BK Virus / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D015266:Carcinoma, Merkel Cell / Q000821:virology*; D002460:Cell Line; D003429:Cross Reactions; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D007313:Insecta; D008297:Male; D018862:Merkel Cells / Q000821:virology; D051379:Mice; D046529:Microscopy, Electron, Transmission; D011120:Polyomavirus / Q000235:genetics / Q000276:immunology*; D027601:Polyomavirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D022701:Virosomes / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D022701:Virosomes