title: Novel TaqMan real-time polymerase chain reaction assay for verifying the authenticity of meat and commercial meat products from game birds.
abstract: Species-specific real-time polymerase chain reaction (PCR) assays using TaqMan probes have been developed for verifying the labeling of meat and commercial meat products from game birds, including quail, pheasant, partridge, guinea fowl, pigeon, Eurasian woodcock and song thrush. The method combines the use of species-specific primers and TaqMan probes that amplify small fragments (amplicons <150 base pairs) of the mitochondrial 12S rRNA gene, and an endogenous control primer pair that amplifies a 141-bp fragment of the nuclear 18S rRNA gene from eukaryotic DNA. Analysis of experimental raw and heat-treated binary mixtures as well as of commercial meat products from the target species demonstrated the suitability of the assay for the detection of the target DNAs.
authors.affiliation: Departamento de Nutricion, Bromatologia y Tecnologia de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Rojas
authors.forename: Isabel
authors.initials: I
authors.lastname: González
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Pavón
authors.forename: Nicolette
authors.initials: N
authors.lastname: Pegels
authors.forename: Adriana
authors.initials: A
authors.lastname: Lago
authors.forename: Pablo E
authors.initials: PE
authors.lastname: Hernández
authors.forename: Teresa
authors.initials: T
authors.lastname: García
authors.forename: Rosario
authors.initials: R
authors.lastname: Martín
mesh_terms: D000818:Animals; D001717:Birds / Q000235:genetics; D002417:Cattle; D004247:DNA / Q000235:genetics; D017931:DNA Primers; D003670:Deer; D005515:Food Labeling / Q000331:legislation & jurisprudence / Q000592:standards; D008460:Meat / Q000592:standards*; D008461:Meat Products / Q000592:standards*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011784:Quail / Q000235:genetics; D012680:Sensitivity and Specificity; D012756:Sheep; D020308:Songbirds / Q000235:genetics; D013552:Swine
chemical_list: D017931:DNA Primers; D004247:DNA