Very stable end-sealed double stranded DNA by click chemistry.
An efficient and simple method has been established for the intermolecular click ligation of two complementary DNA strands to produce an end-sealed duplex with a triazole linkage at each end. The resultant end-sealed duplex is thermally very stable (DeltaT(m) approximately 30 degrees C relative to a normal duplex) and a fluorescent version remained intact for up to 3 days in Fetal Bovine serum. In contrast a single strand was completely degraded in 2 hours. These favorable properties suggest that such cyclic DNA duplexes might have potential for in vivo applications and nanotechnology.
El-Sagheer
Afaf H
AH
School of Chemistry, University of Southampton, Highfield Southampton, United Kingdom. ahes@soton.ac.uk
D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D013696:Temperature; D014230:Triazoles / Q000737:chemistry*
D014230:Triazoles; D004247:DNA