title: A study on the venom of Bothrops alternatus Duméril, Bibron and Duméril, from different regions of Argentina.
abstract: We report a comparison of toxic and enzymatic activities of Bothrops alternatus venoms collected from specimens of different regions of Argentina with a pool of these same venoms. We determined lethal, hemorrhagic and pro-coagulant (plasma and fibrinogen) doses, as well as indirect hemolysis, hydrolysis of gelatin and fibrinogen. The electrophoretic pattern of the venoms and that of fibrinogen incubated with each of the venoms was studied by SDS-PAGE. The toxic activities were consistent with those reported for these venoms but with noticeable differences between samples which do not seem related to geographical or climatic characteristics. In some cases the pooled venom showed values close to the individual samples, but it was not representative since at least the half of the venoms (4/9) exhibited very different patterns of toxicity. The potency of some activities in the individual venoms ranged from much higher to much lower than those determined in the pooled venom (0.1 to 3 fold). Not all venoms exhibited thrombin-like activity but all hydrolyzed alpha and beta chains of fibrinogen; however, degradation patterns were not always similar. Variation in activities and toxicities do not seem to be related to geographical or climatic characteristics.
authors.affiliation: Instituto Nacional de Producción de Biológicos A.N.L.I.S., Ministerio de Salud, Av. Vélez Sarsfield 563, 1281, Buenos Aires, Argentina.
authors.forename: Laura C
authors.initials: LC
authors.lastname: Lanari
authors.forename: Sergio
authors.initials: S
authors.lastname: Rosset
authors.forename: María E
authors.initials: ME
authors.lastname: González
authors.forename: Natalia
authors.initials: N
authors.lastname: Liria
authors.forename: Adolfo R
authors.initials: AR
authors.lastname: de Roodt
mesh_terms: D000818:Animals; D001118:Argentina; D001777:Blood Coagulation / Q000187:drug effects; D017837:Bothrops / Q000502:physiology*; D002417:Cattle; D003435:Crotalid Venoms / Q000737:chemistry / Q000201:enzymology* / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000187:drug effects / Q000378:metabolism; D005780:Gelatin / Q000187:drug effects / Q000378:metabolism; D006470:Hemorrhage / Q000139:chemically induced / Q000473:pathology; D006868:Hydrolysis / Q000187:drug effects; D007928:Lethal Dose 50; D051379:Mice; D011506:Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity
chemical_list: D003435:Crotalid Venoms; D011506:Proteins; D005780:Gelatin; D005340:Fibrinogen