title: Overexpression of NYGGF4 (PID1) induces mitochondrial impairment in 3T3-L1 adipocytes.
abstract: NYGGF4 is a recently discovered gene that is involved in obesity-associated insulin resistance. The exact mechanism by which NYGGF4 induces insulin resistance has not yet been fully elucidated. In this study, we demonstrated that the overexpression of NYGGF4 in 3T3-L1 adipocytes decreased mitochondrial mass, mitochondrial DNA, and intracellular ATP synthesis. In addition, NYGGF4 overexpression also led to an imbalance of the mitochondrial dynamics and excess intracellular ROS production. Collectively, our results indicated that the overexpression of NYGGF4 caused mitochondrial dysfunction in adipocytes, which might be responsible for the development of NYGGF4-induced insulin resistance.
authors.affiliation: Department of Endocrinology, The 82nd Hospital of the People's Liberation Army, No. 100 Jiankang East Road, 223001 Huaian, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zhao
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Chen
authors.forename: Chunlin
authors.initials: C
authors.lastname: Gao
authors.forename: Chenbo
authors.initials: C
authors.lastname: Ji
authors.forename: Fukun
authors.initials: F
authors.lastname: Chen
authors.forename: Chun
authors.initials: C
authors.lastname: Zhu
authors.forename: Jingai
authors.initials: J
authors.lastname: Zhu
authors.forename: Jialin
authors.initials: J
authors.lastname: Wang
authors.forename: Lingmei
authors.initials: L
authors.lastname: Qian
authors.forename: Xirong
authors.initials: X
authors.lastname: Guo
mesh_terms: D041721:3T3-L1 Cells; D000255:Adenosine Triphosphate / Q000378:metabolism; D017667:Adipocytes / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000378:metabolism; D007333:Insulin Resistance / Q000235:genetics; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D017382:Reactive Oxygen Species / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors; D014162:Transfection; D015854:Up-Regulation
chemical_list: D002352:Carrier Proteins; D004272:DNA, Mitochondrial; C514653:PID1 protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D017382:Reactive Oxygen Species; D015534:Trans-Activators; D014157:Transcription Factors; D000255:Adenosine Triphosphate