Measurement of milk D-3-hydroxybutyrate with a simple UV spectrophotometer method: an alternative assay method.
The measurement of D-3-hydroxybutyrate (D-BHBA) in milk samples is an important tool for diagnosis of subclinical/clinical ketosis in dairy cows. We describe a simple UV spectrophotometric method for measuring the concentration of D-BHBA in milk of dairy cows. From two herds, 119 milk samples were taken from dairy cows. The standard-curve equation was y = 0.2582x + 0.0269 (R2 = 0.9967). The assay was highly specific with a minimum detection limit of 0.01 mmol/L and measuring range of up to 5 mmol/L. The recovery was between 99.35% and 100.22% and repeatability was 99.8%. The comparison between the spectrophotometric method and the fluorometric method revealed a close correlation (r = 0.9939). These results show that the spectrophotometric method can be successfully used as an alternative method to measure D-BHBA content in milk.
Zhang
Z G
ZG
College of Animal Science and Veterinary Medicine, Jilin University, 5333 of Xi'an Road, Changchun, Jilin, 130062, China.
Liu
G W
GW
Li
X B
XB
Li
Y F
YF
Guo
C M
CM
Gao
L
L
Wang
H B
HB
Xu
S W
SW
Wang
Z
Z
D020155:3-Hydroxybutyric Acid / Q000032:analysis*; D000818:Animals; D002417:Cattle; D005260:Female; D008892:Milk / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods / Q000662:veterinary*
D020155:3-Hydroxybutyric Acid