title: Study on the molecularly imprinted polymers with methyl-testosterone as the template.
abstract: Molecularly imprinted polymers (MIPs) using methyl-testosterone as the template, methacrylic acid (MAA) as the monomer and ethylene glycol dimethacrylate (EDMA) as the crosslinker were prepared by precipitation polymerization. The morphology of the obtained particles was characterized by scanning electron microscopy (SEM) and the pore size was measured by BET. Then, the specificity and selectivity of the MIPs were evaluated using the equilibrium rebinding experiments. Besides, the MIPs were also used as the stationary phase of HPLC column and the retention behaviour to the template and analogues was confirmed using HPLC-MS-MS. Finally, the real application of the methyl-testosterone imprinted polymers was evaluated using SPE procedure with the spiked tap water and lake water. The results indicated that the prepared methyl-testosterone imprinted polymer showed specific rebinding ability to its template and could retain the template strongly compared with other structural analogues. At the same time, the MIPs could be used as SPE column to enrich methyl-testosterone in the lake water and show broad prospects in real samples.
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, No 3, Gaobeidian North Road, Chaoyang District, Beijing 100123, PR China.
authors.forename: Minli
authors.initials: M
authors.lastname: Yang
authors.forename: Wancheng
authors.initials: W
authors.lastname: Gu
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Ling
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Chu
authors.forename: Daning
authors.initials: D
authors.lastname: Wang
mesh_terms: D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D008777:Methyltestosterone / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D011109:Polymethacrylic Acids / Q000138:chemical synthesis* / Q000737:chemistry*; D052616:Solid Phase Extraction; D013379:Substrate Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D008777:Methyltestosterone