Quality control and reproducibility in DNA microarray experiments.
Biological experiments are usually set up in technical replicates (duplicates or triplicates) in order to ensure reproducibility and, to assess any significant error introduced during the experimental process. The first step in biological data analysis is to check the technical replicates and to confirm that the error of measure is small enough to be of no concern. However, little attention has been paid to this part of analysis. Here, we propose a general process to estimate the error of measure and consequently, to provide an interpretable and objective way to ensure the technical replicates' quality. Particularly, we illustrate our application in a DNA microarray dataset set up in technical duplicates.
Fujita
André
A
Computational Science Research Program, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. andrefujita@riken.jp
Sato
João R
JR
DA Silva
Fernando H L
FH
Galvão
Maria C
MC
Sogayar
Mari C
MC
Miyano
Satoru
S
D020411:Oligonucleotide Array Sequence Analysis; D011786:Quality Control; D015203:Reproducibility of Results