Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells.
Dendritic cells (DC) represent a heterogeneous cell family of major importance for innate immune responses against pathogens and antigen presentation during infection, cancer, allergy and autoimmunity. The aim of the present study was to characterize canine DC generated in vitro with respect to their phenotype, responsiveness to toll-like receptor (TLR) ligands and T-cell stimulatory capacity. DC were derived from monocytes (MoDC) and from bone marrow hematopoietic cells cultured with either Flt3-ligand (FL-BMDC) or with GM-CSF (GM-BMDC). All three methods generated cells with typical DC morphology that expressed CD1c, CD11c and CD14, similar to macrophages. However, CD40 was only found on DC, CD206 on MPhi and BMDC, but not on monocytes and MoDC. CD1c was not found on monocytes but on all in vitro differentiated cells. FL-BMDC and GM-BMDC were partially positive for CD4 and CD8. CD45RA was expressed on a subset of FL-BMDC but not on MoDC and GM-BMDC. MoDC and FL-DC responded well to TLR ligands including poly-IC (TLR2), Pam3Cys (TLR3), LPS (TLR4) and imiquimod (TLR7) by up-regulating MHC II and CD86. The generated DC and MPhi showed a stimulatory capacity for lymphocytes, which increased upon maturation with LPS. Taken together, our results are the basis for further characterization of canine DC subsets with respect to their role in inflammation and immune responses.
Ricklin Gutzwiller
Meret Elisabeth
ME
Division of Clinical Dermatology, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Switzerland. meret.ricklin@kkh.unibe.ch
Moulin
Hervé Raphaël
HR
Zurbriggen
Andreas
A
Roosje
Petra
P
Summerfield
Artur
A
D000818:Animals; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003713:Dendritic Cells / Q000166:cytology* / Q000502:physiology*; D004285:Dogs; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D009000:Monocytes / Q000166:cytology* / Q000502:physiology*; D011994:Recombinant Proteins; D051941:fms-Like Tyrosine Kinase 3
D015415:Biomarkers; D011994:Recombinant Proteins; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D051941:fms-Like Tyrosine Kinase 3