Evaluation of the effects of ozone therapy in the treatment of intra-abdominal infection in rats.
BACKGROUND
The antibacterial effect of ozone (O(3)) has been described in the extant literature, but the role of O(3) therapy in the treatment of certain types of infection remains controversial.


OBJECTIVE
To evaluate the effect of intraperitoneal (i.p.) O(3) application in a cecal ligation/puncture rat model on interleukins (IL-6, IL-10) and cytokine-induced neutrophil chemoattractant (CINC)-1 serum levels, acute lung injury and survival rates.


METHODS
FOUR ANIMAL GROUPS WERE USED FOR THE STUDY: a) the SHAM group underwent laparotomy; b) the cecal ligation/puncture group underwent cecal ligation/puncture procedures; and c) the CLP+O(2) and CLP+O(3) groups underwent CLP+ corresponding gas mixture infusions (i.p.) throughout the observation period. IL-6, CINC-1 and IL-10 concentrations were determined by enzyme-linked immunosorbent assay (ELISA). Acute lung injury was evaluated with the Evans blue dye lung leakage method and by lung histology. P<0.05 was considered significant.


RESULTS
CINC-1 was at the lowest level in the SHAM group and was lower for the CLP+O(3) group vs. the CLP+O(2) group and the cecal ligation/puncture group. IL-10 was lower for the SHAM group vs. the other three groups, which were similar compared to each other. IL-6 was lower for the SHAM group vs. all other groups, was lower for the CLP+O(3) or CLP+O(2) group vs. the cecal ligation/puncture group, and was similar for the CLP+O(3) group vs. the CLP+O(2) group. The lung histology score was lower for the SHAM group vs. the other groups. The Evans blue dye result was lower for the CLP+O(3) group vs. the CLP+O(2) group and the cecal ligation/puncture group but similar to that of the SHAM group. The survival rate for the CLP+O(3) group was lower than for the SHAM group and similar to that for the other 2 groups (CLP and CLP+O(2)).


CONCLUSIONS
Ozone therapy modulated the inflammatory response and acute lung injury in the cecal ligation/puncture infection model in rats, although there was no improvement on survival rates.
Souza
Yglesio Moyses de
YM
Faculdade de Medicina da Universidade de São Paulo - São Paulo/SP, Brazil. yglesio@yahoo.com
Fontes
Belchor
B
Martins
Joilson O
JO
Sannomiya
Paulina
P
Brito
Glacus S
GS
Younes
Riad N
RN
Rasslan
Samir
S
D000818:Animals; D002432:Cecum / Q000601:surgery; D054360:Chemokine CXCL1 / Q000097:blood*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D016753:Interleukin-10 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D008026:Ligation; D008297:Male; D010126:Ozone / Q000627:therapeutic use*; D010538:Peritonitis / Q000097:blood / Q000188:drug therapy*; D011677:Punctures; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000097:blood / Q000188:drug therapy*
D054360:Chemokine CXCL1; C513297:Cxcl1 protein, rat; D015850:Interleukin-6; D016753:Interleukin-10; D010126:Ozone