Determination of methocarbamol concentration in human plasma by high performance liquid chromatography-tandem mass spectrometry.
A simple and reproducible high performance liquid chromatography-tandem mass spectrometric method was developed for methocarbamol analysis in human plasma. Methocarbamol and the internal standard (IS) were extracted by a protein precipitation method. Under isocratic separation condition the chromatographic run time was 3.0 min. The calibration curve was linear over a range of 150-12,000 ng/mL with good intraday assay and interday assay precision (CV%<10.9%). The method was proven to be sensitive and selective for the analysis of methocarbamol in human plasma for bioequivalence study.
Zha
Wuyi
W
Pharmascience, 6111 Royalmount Ave, Montreal, Quebec H4P 2T4, Canada.
Zhu
Zhimeng
Z
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008721:Methocarbamol / Q000097:blood*; D009125:Muscle Relaxants, Central / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D009125:Muscle Relaxants, Central; D008721:Methocarbamol