LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication.
LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3'-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions.
Galão
Rui Pedro
RP
Department of Experimental and Health Sciences, Universitat Pompeu Fabra, 08003 Barcelona, Spain.
Chari
Ashwin
A
Alves-Rodrigues
Isabel
I
Lobão
Daniela
D
Mas
Antonio
A
Kambach
Christian
C
Fischer
Utz
U
Díez
Juana
J
D020413:3' Untranslated Regions; D001665:Binding Sites; D017795:Bromovirus / Q000235:genetics / Q000378:metabolism; D016679:Genome, Viral; D014176:Protein Biosynthesis; D039381:RNA Cap-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D019065:Virus Assembly; D014779:Virus Replication / Q000502:physiology*
D020413:3' Untranslated Regions; C114069:LSM1 protein, S cerevisiae; C521832:LSM7 protein, S cerevisiae; D039381:RNA Cap-Binding Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins