Novel expression patterns of connexin 30.3 in adult rat cochlea.
Mutations of the GJB4 gene, encoding connexin 30.3 (CX30.3), are associated with skin disorders. Recently, this gene was also detected in deaf individuals without skin disorders. However, the functional roles of CX30.3 in the cochlea remain unclear. A primary step toward understanding the role of CX30.3 in hearing and its dysfunction is the documentation of its cellular and sub-cellular locations within the cochlea. In the present study, we located and determined the cellular expression of Cx30.3 within the rat cochlea by using a polyclonal anti-Cx30.3 antibody. Expression of the Cx30.3 protein was detected in the spiral limbus, spiral ligament, spiral ganglion, and stria vascularis by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) analyses. Our results indicate the presence and localization of Cx30.3 in the rat cochlea. Knowledge of the spatial distribution of Cx30.3 will provide important insights into its role in the cochleae and normal auditory function.
Wang
Wen-Hung
WH
Department of Otolaryngology, Chang Gung Memorial Hospital at Chia-yi, Taiwan, ROC.
Yang
Jiann-Jou
JJ
Lin
Yen-Chun
YC
Yang
Jen-Tsung
JT
Li
Shuan-Yow
SY
D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D003051:Cochlea / Q000737:chemistry*; D017630:Connexins / Q000032:analysis* / Q000235:genetics; D007150:Immunohistochemistry; D007834:Lasers; D008297:Male; D042282:Microdissection / Q000295:instrumentation; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013136:Spiral Ganglion / Q000737:chemistry; D055493:Spiral Ligament of Cochlea / Q000737:chemistry; D013316:Stria Vascularis / Q000737:chemistry
D017630:Connexins; D012333:RNA, Messenger; C076275:connexin 30.3