Prophylactic role of a herbomineral drug "Thamira parpam" against cysteamine-induced oxidative stress in liver and duodenum of rats.
Copper is known as Gunma Kaalan in Siddha literature, which means that the drug is effective for healing ulcers. The herbomineral drug "Thamira parpam" is prepared by calcining the purified copper foils with rock salt, lime juice, bracteated birth wort juice, and Alangium root decoction according to Siddha medicine. Our study investigated the possible role of Thamira parpam (TP) in the management of cysteamine-induced duodenal ulcers. Cysteamine (400 mg kg(-1) body weight(-1), two doses at 4 h interval) orally given to rats resulted in high ulcer index, increased TBARS with concomitant depletion of antioxidants such as superoxide dismutase, glutathione, glutathione peroxidase, and inflammatory markers cathepsin D, and myeloperoxidase (p < 0.01). Herbomineral drug TP (0.5, 1, and 2 mg/kg, p.o.) challenged with cysteamine attenuated the elevation of TBARS and imbalance of antioxidants. In the increases in liver inflammatory markers, tissue histopathology changes were not severe in TP treatment. Positive control omeprazole (25 mg/kg, body weight, orally) showed considerable protection against anomaly in biochemical parameters and tissue histology. Hence, our results indicate that the attenuation of oxidative stress by the herbomineral drug in experimentally induced damage to liver and duodenum of rats could be mediated by free radical quenching property.
Parameswari
R P
RP
Herbal and Indian Medicine Research Laboratory, Department of Biochemistry, Sri Ramachandra University, Porur, Chennai 600116, India.
Vasanthkumar
M
M
Gayathri
V
V
Manikandamathavan
V M
VM
Ramakrishnan
G
G
Sangeetha
M K
MK
Vijayakumar
V
V
Raghavendran
H Balaji
HB
Chamundeeswari
D
D
Vasanthi
Hannah R
HR
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002402:Cathepsin D / Q000378:metabolism; D003543:Cysteamine / Q000633:toxicity*; D004381:Duodenal Ulcer / Q000139:chemically induced* / Q000378:metabolism; D004386:Duodenum / Q000187:drug effects / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000633:toxicity; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
D010936:Plant Extracts; D011837:Radiation-Protective Agents; C549498:thamira parpam; D003543:Cysteamine; D009195:Peroxidase; D013482:Superoxide Dismutase; D002402:Cathepsin D; D005978:Glutathione