Microbial biofilm growth versus tissue integration on biomaterials with different wettabilities and a polymer-brush coating.
Biomaterials-associated infections (BAI) constitute a major clinical problem and often necessitate implant replacement. In this study, the race for the surface between Staphylococcus epidermidis ATCC 35983 and U2OS osteosarcoma cells is studied on biomaterials with different wettabilities and on a polymer-brush coating. S. epidermidis was deposited on the different surfaces in a parallel plate flow chamber and then U2OS cells were seeded. Subsequently, staphylococci and U2OS cells were allowed to grow simultaneously on the surfaces for 48 h under low flow conditions. The presence of staphylococci reduced cell growth on all surfaces, but adhering cells spread equally well in the absence and presence of staphylococci. A hydrophilic polymer-brush coating discouraged bacterial and cellular adhesion and growth. Thus, whereas the biomaterials evaluated support both biofilm formation and tissue integration, polymer-brush coatings support neither. Therewith, the outcome of the race for the surface on these surfaces remains uncertain, emphasizing the need for biofunctionalized surfaces that discourage biofilm formation and support tissue growth at the same time.
Subbiahdoss
Guruprakash
G
Department of Biomedical Engineering, University Medical Center Groningen, Groningen and University of Groningen, P.O. Box 196, 9700 AD Groningen, The Netherlands.
Grijpma
Dirk W
DW
van der Mei
Henny C
HC
Busscher
Henk J
HJ
Kuijer
Roel
R
D018441:Biofilms / Q000254:growth & development*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D011108:Polymers / Q000737:chemistry*; D019736:Prostheses and Implants / Q000382:microbiology; D013203:Staphylococcal Infections / Q000517:prevention & control; D013212:Staphylococcus epidermidis / Q000166:cytology / Q000254:growth & development; D013499:Surface Properties; D017155:Wettability
D020099:Coated Materials, Biocompatible; D011108:Polymers