Intra-uterine tissue engineering of full-thickness skin defects in a fetal sheep model.
In spina bifida the neural tube fails to close during the embryonic period and it is thought that prolonged exposure of the unprotected spinal cord to the amniotic fluid during pregnancy causes additional neural damage. Intra-uterine repair might protect the neural tissue from exposure to amniotic fluid and might reduce additional neural damage. Biodegradable collagen scaffolds may be useful in case of fetal therapy for spina bifida, but biochemical properties need to be studied. The aim of this study was to investigate whether biodegradable collagen scaffolds can be used to treat full-thickness fetal skin defects. We hypothesized that the pro-angiogenic growth factors VEGF and FGF2 would enhance vascularization, epidermialization and lead to improved wound healing. To investigate the effect of these two growth factors, a fetal sheep model for skin defects was used. Compared to wounds treated with bare collagen scaffolds, wounds treated with growth factor-loaded scaffolds showed excessive formation of capillaries and less myofibroblasts were present in these wounds, leading to less contraction. This study has demonstrated that collagen scaffolds can be used to treat fetal skin defects and that the combination of collagen scaffolds with VEGF and FGF2 had a beneficial effect on wound healing.
Hosper
Nynke A
NA
University Medical Center Groningen, University of Groningen, Department of Pathology and Medical Biology, Groningen, The Netherlands. n.a.hosper@med.umcg.nl
Eggink
Alex J
AJ
Roelofs
Luc A J
LA
Wijnen
Rene M H
RM
van Luyn
Marja J A
MJ
Bank
Ruud A
RA
Harmsen
Martin C
MC
Geutjes
Paul J
PJ
Daamen
Willeke F
WF
van Kuppevelt
Toin H
TH
Tiemessen
Dorien M
DM
Oosterwijk
Egbert
E
Crevels
Jane J
JJ
Blokx
Willeke A M
WA
Lotgering
Fred K
FK
van den Berg
Paul P
PP
Feitz
Wout F J
WF
D000818:Animals; D002417:Cattle; D004848:Epithelium / Q000473:pathology; D005260:Female; D005333:Fetus / Q000473:pathology* / Q000601:surgery; D005347:Fibroblasts / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D009389:Neovascularization, Pathologic; D011247:Pregnancy; D012756:Sheep / Q000601:surgery; D012867:Skin / Q000098:blood supply / Q000473:pathology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014599:Uterus; D014945:Wound Healing