An improved protocol for the preparation of total genomic DNA from isolates of yeast and mould using Whatman FTA filter papers.
Here, we present a significantly improved version of our previously published method for the extraction of fungal genomic DNA from pure cultures using Whatman FTA filter paper matrix technology. This modified protocol is extremely rapid, significantly more cost effective than our original method, and importantly, substantially reduces the problem of potential cross-contamination between sequential filters when employing FTA technology.
Borman
Andrew M
AM
Mycology Reference Laboratory, Health Protection Agency, South-West Regional Laboratory, Bristol, UK. Andy.Borman@uhBristol.nhs.uk
Fraser
Mark
M
Linton
Christopher J
CJ
Palmer
Michael D
MD
Johnson
Elizabeth M
EM
D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification*; D005374:Filtration / Q000295:instrumentation*; D005658:Fungi / Q000235:genetics / Q000302:isolation & purification; D016681:Genome, Fungal; D006801:Humans; D009181:Mycoses / Q000175:diagnosis / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D013048:Specimen Handling; D013997:Time Factors; D015003:Yeasts / Q000235:genetics / Q000302:isolation & purification
D003470:Culture Media; D004271:DNA, Fungal