[Using high-density universal 16S rRNA microarray in microbial molecular ecology research].
High-density universal 16S rRNA Microarray was used to detect the microbial community in membrane bioreactor (MBR). The results showed MBR had high microbial diversity. 1019 bacteria were detected by Microarray in MBR. Proteobacteria was the predominant phylum. It had 657 OTUs, occupying 64.5% of the total bacteria, gamma Proteobacteria was the main class of Proteobacteria, which occupied 35.8% of Proteobacteria, but the average fluorescence intensity of was not very high. Despite the bacteria diversity of beta Proteobacteria was lower than gamma Proteobacteria. However, it occupied 40% and 36% in the 25 and 50 dominant bacteria according to relative average fluorescence intensity. Clostridia had a relative high concentration when compare the average fluorescence intensity of the whole bacteria in MBR. Some common nitrify bacteria such as Nitrosomonadaceae and Nitrospiraceae were also detected and had high fluorescence intensity in the system. Microarray is a newer molecular method and has some advantages such as real time, high efficiency and exact results. It's very suitable for investigation of microbial diversity in wastewater treatment.
Duan
Liang
L
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. liangduan@gmail.com
Xia
Si-qing
SQ
Song
Yong-hui
YH
Piceno
Yvette M
YM
Hermanowicz
Slawomir W
SW
D001419:Bacteria / Q000145:classification* / Q000235:genetics; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology*; D004463:Ecology / Q000379:methods; D008567:Membranes, Artificial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020560:Proteobacteria / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D014865:Waste Disposal, Fluid / Q000379:methods
D008567:Membranes, Artificial; D012336:RNA, Ribosomal, 16S