Chalcones enhance TRAIL-induced apoptosis in prostate cancer cells.
Chalcones exhibit chemopreventive and antitumor effects. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a naturally occurring anticancer agent that induces apoptosis in cancer cells and is not toxic to normal cells. We examined the cytotoxic and apoptotic effect of five chalcones in combination with TRAIL on prostate cancer cells. The cytotoxicity was evaluated by the MTT and LDH assays. The apoptosis was determined using flow cytometry with annexin V-FITC. Our study showed that all five tested chalcones: chalcone, licochalcone-A, isobavachalcone, xanthohumol, butein markedly augmented TRAIL-mediated apoptosis and cytotoxicity in prostate cancer cells and confirmed the significant role of chalcones in chemoprevention of prostate cancer.
Szliszka
Ewelina
E
Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana, Zabrze, Poland. eszliszka@sum.edu.pl
Czuba
Zenon P
ZP
Mazur
Bogdan
B
Sedek
Lukasz
L
Paradysz
Andrzej
A
Krol
Wojciech
W
D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D047188:Chalcones / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000188:drug therapy*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000627:therapeutic use*
D016588:Anticarcinogenic Agents; D047188:Chalcones; D053221:TNF-Related Apoptosis-Inducing Ligand