Determination of xanthohumol in beer based on cloud point extraction coupled with high performance liquid chromatography.
A method based on coupling of cloud point extraction (CPE) with high performance liquid chromatography separation and ultraviolet detection was developed for determination of xanthohumol in beer. The nonionic surfactant Triton X-114 was chosen as the extraction medium. The parameters affecting the CPE were evaluated and optimized. The highest extraction yield of xanthohumol was obtained with 2.5% of Triton X-114 (v/v) at pH 5.0, 15% of sodium chloride (w/v), 70 degrees C of equilibrium temperature and 10 min of equilibrium time. Under these conditions, the limit of detection of xanthohumol is 0.003 mg L(-1). The intra- and inter-day precisions expressed as relative standard deviations are 4.6% and 6.3%, respectively. The proposed method was successfully applied for determination of xanthohumol in various beer samples. The contents of xanthohumol in these samples are in the range of 0.052-0.628 mg L(-1), and the recoveries ranging from 90.7% to 101.9% were obtained. The developed method was demonstrated to be efficient, green, rapid and inexpensive for extraction and determination of xanthohumol in beer.
Chen
Ligang
L
College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, China.
Zhao
Qi
Q
Jin
Haiyan
H
Zhang
Xiaopan
X
Xu
Yang
Y
Yu
Aimin
A
Zhang
Hanqi
H
Ding
Lan
L
D053000:Analytic Sample Preparation Methods; D001515:Beer / Q000032:analysis*; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005240:Feasibility Studies; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification*; D005504:Food Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000737:chemistry; D011427:Propiophenones / Q000032:analysis* / Q000302:isolation & purification*; D012965:Sodium Chloride / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D005419:Flavonoids; D011427:Propiophenones; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D017830:Octoxynol; C010615:Nonidet P-40; C104536:xanthohumol