Autophagosomes can support Yersinia pseudotuberculosis replication in macrophages.
Yersinia pseudotuberculosis is able to replicate inside macrophages. However, the intracellular trafficking of the pathogen after its entry into the macrophage remains poorly understood. Using in vitro infected bone marrow-derived macrophages, we show that Y. pseudotuberculosis activates the autophagy pathway. Host cell autophagosomes subverted by bacteria do not become acidified and sustain bacteria replication. Moreover, we report that autophagy inhibition correlated with bacterial trafficking inside an acidic compartment. This study indicates that Y. pseudotuberculosis hijacks the autophagy pathway for its replication and also opens up new opportunities for deciphering the molecular basis of the host cell signalling response to intracellular Yersinia infection.
Moreau
Kevin
K
Cellular Microbiology of Infectious Pathogens, Center of Infection and Immunity of Lille, Institut Pasteur de Lille, Lille, France.
Lacas-Gervais
Sandra
S
Fujita
Naonobu
N
Sebbane
Florent
F
Yoshimori
Tamotsu
T
Simonet
Michel
M
Lafont
Frank
F
D000818:Animals; D001343:Autophagy; D005260:Female; D057131:Immune Evasion; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010588:Phagosomes / Q000382:microbiology*; D015011:Yersinia pseudotuberculosis / Q000254:growth & development / Q000472:pathogenicity*