Influence of an adenosine deaminase inhibitor, erythro-9-(2-hydroxy-3-nonyl) adenine hydrochloride, on 5-HT2CR mRNA editing in primary cultured cortical cells.
Treatment of primary cultured cortical cells with erythro-9-(2-hydroxy-3-nonyl) adenine hydrochloride (EHNA), an inhibitor of adenosine deaminase (ADAR), for 6 d significantly and concentration-dependently reduced the editing efficacy at sites C and D but not at site A or B of 5-HT2CR mRNA. The treatment failed to affect the editing of ADAR-2 pre-mRNA and a subunit of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor (GluR2) mRNA. These findings suggest that EHNA is useful for clarifying the functional roles of 5-HT2CR mRNA editing at sites C and D.
Pham
Nguyet Thi Hang
NT
Division of Medicinal Pharmacology, Institute of Natural Medicine, University of Toyama, Japan.
Tohda
Michihisa
M
Tezuka
Yasuhiro
Y
Matsumoto
Kinzo
K
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D058892:Adenosine Deaminase Inhibitors; D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects*; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional / Q000187:drug effects*; D051381:Rats; D044405:Receptor, Serotonin, 5-HT2C / Q000235:genetics; D017470:Receptors, Glutamate / Q000235:genetics / Q000378:metabolism*; D058830:Serotonin 5-HT2 Receptor Antagonists
D058892:Adenosine Deaminase Inhibitors; D012322:RNA Precursors; D044405:Receptor, Serotonin, 5-HT2C; D017470:Receptors, Glutamate; D058830:Serotonin 5-HT2 Receptor Antagonists; C010192:9-(2-hydroxy-3-nonyl)adenine; D000225:Adenine