Donor-derived DNA in hair follicles of recipients after allogeneic hematopoietic stem cell transplantation.
The hair follicles of recipients of allogeneic hematopoietic SCT (HSCT) constitute the tissue with the greatest need for regeneration after high-dose chemotherapy. Previous studies have shown a lack of donor-derived DNA in the hair follicles of recipients. Therefore, we carried out a study to determine whether male donor-derived genetic material can be found in female recipients' hair follicles after HSCT. Fluorescent-based PCR with analyses of Y-chromosome STR (Y-STR) and RQ-PCR with the sex-determining region Y (SRY) were used independently to evaluate chimerism status. Our results proved the existence of donor-derived stem DNA in the recipients' hair follicle cells. This report undermines the validity of data indicating that hair follicle cells maintain 100% of recipient origin.
Jacewicz
R
R
Forensic Genetics Laboratory, Department of Forensic Medicine, Medical University of Lodz, Sedziowska 18a, Lodz, Poland. r.jacewicz@post.pl
Lewandowski
K
K
Rupa-Matysek
J
J
Jedrzejczyk
M
M
Brzezinski
P M
PM
Dobosz
T
T
Jonkisz
A
A
Szram
S
S
Komarnicki
M
M
Berent
J
J
D000328:Adult; D041322:Chromosomes, Human, Y; D004247:DNA / Q000032:analysis / Q000235:genetics*; D005260:Female; D006085:Graft Survival; D018859:Hair Follicle / Q000737:chemistry / Q000502:physiology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D014019:Tissue Donors; D018183:Transplantation Chimera; D055815:Young Adult
D004247:DNA