Hybrid superparamagnetic iron oxide nanoparticle-branched polyethylenimine magnetoplexes for gene transfection of vascular endothelial cells.
The work demonstrated the development of thermally cross-linked superparamagnetic nanomaterial which possessed polyethylene glycol moiety and covalently linked branched polyethylenimine (BPEI), and exhibited highly efficient magnetofection even under serum conditioned media. The study showed its high anti-biofouling, cell viability and serum stability and thus revealed a potential magnetic nanoparticle-mediated targeted gene delivery system. This superparamagnetic particle mediated rapid and efficient transfection in primary vascular endothelial cells (HUVEC) successfully inhibits expression of PAI-1 which is responsible for various vascular dysfunctions such as vascular inflammation and atherosclerosis and thereby provides a potential strategy to transfect highly sensitive HUVEC. The sequential steps for the enhanced magnetofection had been studied by monitoring cellular uptake with the aid of confocal microscopy.
Namgung
Ran
R
Department of Chemistry, BK21 Program, Polymer Research Institute, Pohang University of Science and Technology, Pohang, Republic of Korea.
Singha
Kaushik
K
Yu
Mi Kyung
MK
Jon
Sangyong
S
Kim
Yong Sook
YS
Ahn
Youngkeun
Y
Park
In-Kyu
IK
Kim
Won Jong
WJ
D045744:Cell Line, Tumor; D002470:Cell Survival; D004247:DNA / Q000378:metabolism; D003911:Dextrans; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology*; D052203:Ferrosoferric Oxide / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D008280:Magnetics / Q000379:methods*; D058185:Magnetite Nanoparticles; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010957:Plasmids / Q000235:genetics; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D011094:Polyethyleneimine / Q000378:metabolism*; D013499:Surface Properties; D013997:Time Factors; D014162:Transfection / Q000379:methods*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D003911:Dextrans; D058185:Magnetite Nanoparticles; D017395:Plasminogen Activator Inhibitor 1; D014409:Tumor Necrosis Factor-alpha; D011094:Polyethyleneimine; D004247:DNA; C065507:ferumoxides; D016650:Fluorescein-5-isothiocyanate; D052203:Ferrosoferric Oxide