Gene expression profile of human skeletal muscle and adipose tissue of Chinese Han patients with type 2 diabetes mellitus.
OBJECTIVE
To study the differential patterns of gene expression in skeletal muscle and adipose tissue between type 2 diabetes mellitus (T2DM) patients and healthy subjects using DNA microarray analysis.


METHODS
T2DM patiens were divided into female group, young male group and old male group. DNA microarray analysis and quantitative real-time PCR were carried out to analyze the relation between gene expressions and T2DM.


RESULTS
The mRNA expression of 298, 578, and 350 genes was changed in the skeletal muscle of diabetes mellitus patients compared with control subjects. The 1320, 1143, and 2847 genes were modified in adipose tissue of the three groups. Among the genes surveyed, the change of 25 and 39 gene transcripts in skeletal muscle and adipose tissue was > or = 2 folds. These differentially expressed genes were classified into 15 categories according to their functions.


CONCLUSIONS
New genes are found and T2DM can be prevented or cured.
Yang
Yan-Li
YL
Department of Internal Medicine, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China.
Xiang
Ruo-Lan
RL
Yang
Chang
C
Liu
Xiao-Jun
XJ
Shen
Wen-Jun
WJ
Zuo
Jin
J
Chang
Yong-Sheng
YS
Fang
Fu-De
FD
D000273:Adipose Tissue / Q000378:metabolism*; D000368:Aged; D044466:Asian People; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis