title: Insufficient evidence for association of NOD2/CARD15 or other inflammatory bowel disease-associated markers on GVHD incidence or other adverse outcomes in T-replete, unrelated donor transplantation.
abstract: Previous European studies suggest NOD2/CARD15 and interleukin-23 receptor (IL-23R) donor or recipient variants are associated with adverse clinical outcomes in allogeneic hematopoietic stem cell transplantation. We reexamined these findings as well as the role of another inflammatory bowel disease (IBD) susceptibility gene (immunity-related GTPase family, M [IRGM]) on transplantation outcomes in 390 US patients and their matched unrelated donors, accrued between 1995 and 2004. Patients received T-replete grafts with mostly myeloablative conditioning regimens. Multivariate analyses were performed for overall survival, disease-free survival, transplantation-related mortality, relapse, and acute and chronic graft-versus-host disease. Of 390 pairs, NOD2/CARD15 variant single nucleotide polymorphisms (SNPs) were found in 14% of donors and 17% of recipients. In 3% both donor and recipient had a mutant SNP. Thirteen percent of donors and 16% of recipients had variant IL23R SNPs, with 3% having both donor and recipient variants. Twenty-three percent of both donors and recipients had variant IRGM SNPs. None of the 3 IBD-associated alleles showed a statistically significant association with any adverse clinical outcomes. Our results do not support an association between the 3 IBD-associated SNPs and adverse outcomes after matched unrelated donor hematopoietic cell transplantations in US patients.
authors.affiliation: Division of Gastroenterology, Washington University School of Medicine, St Louis, MO, USA.
authors.forename: Yume
authors.initials: Y
authors.lastname: Nguyen
authors.forename: Abed
authors.initials: A
authors.lastname: Al-Lehibi
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Gorbe
authors.forename: Ellen
authors.initials: E
authors.lastname: Li
authors.forename: Michael
authors.initials: M
authors.lastname: Haagenson
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Stephen
authors.initials: S
authors.lastname: Spellman
authors.forename: Stephanie J
authors.initials: SJ
authors.lastname: Lee
authors.forename: Nicholas O
authors.initials: NO
authors.lastname: Davidson
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D018572:Disease-Free Survival; D046148:Donor Selection; D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006086:Graft vs Host Disease / Q000235:genetics* / Q000378:metabolism / Q000401:mortality; D019337:Hematologic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000401:mortality / Q000628:therapy; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015212:Inflammatory Bowel Diseases / Q000235:genetics* / Q000378:metabolism / Q000401:mortality; D019520:Living Donors; D008212:Lymphocyte Depletion; D008297:Male; D008875:Middle Aged; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D012008:Recurrence; D012189:Retrospective Studies; D015996:Survival Rate; D019172:Transplantation Conditioning; D014184:Transplantation, Homologous; D014481:United States
chemical_list: D015415:Biomarkers; C429018:NOD2 protein, human; D053473:Nod2 Signaling Adaptor Protein; D019204:GTP-Binding Proteins; C513539:IRGM protein, human