[Experiment of rhBMP-2 in posterolateral spinal fusion in aged rabbits].
OBJECTIVE
Posterolateral intertransverse process fusion was performed in aged and young adult female rabbits lumbar spine using recombinant human bone morphogenetic protein-2 (rhBMP-2) and autograft to reveal the function of rhBMP-2 on spinal fusion on aged animals.


METHODS
A total of 24 female New Zealand white rabbits included 12 young adult of 6 months and 12 aged of 2-year-old, was divided into 4 groups: (1) young adult autologous iliac crest bone group [ICBG(Y), n=6); (2) young adult rhBMP-2/absorbable collagen sponge (ACS) group [BMP-2(Y), n=6]; (3) aged autologous iliac crest bone group [ICBG(O), n=6]; aged rhBMP-2/ACS group [BMP-2(O), n=6]. All were underwent posterolateral fusion in same day. rhBMP-2 and autologous iliac crest bone was implant bilateral LS-L6 intertransverse processes, respectively. Half of the rabbits were sacrificed at 3.6 weeks following surgery, respectively. The results were assessed by manual palpation, radiographs, computed tomographic scans (3D) and histology.


RESULTS
Six weeks after surgery, radiography, computed tomography and histology indicated the different result in healing in the posterolateral fusion using rhBMP-2 compared to ICBG (P < 0.05). Aged BMP-2 group showed significantly higher fusion rates than Aged ICBG group.


CONCLUSIONS
This study demonstrated rhBMP-2 can increase the posterolateral fusion rate and new bone quality in aged rabbitss than autograft, it may take the place of ICBG. But its role is effected by age.
Duan
Xing-wei
XW
Department of Orthopaedics, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin, China.
Wu
Han
H
Gu
Chang-yue
CY
Yu
Yan
Y
Yang
Xiao-yu
XY
Wei
Jun
J
Duan
De-sheng
DS
D000375:Aging; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D005260:Female; D006801:Humans; D010173:Palpation; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology*; D013116:Spinal Cord / Q000187:drug effects* / Q000473:pathology / Q000601:surgery* / Q000637:transplantation; D013123:Spinal Fusion; D014057:Tomography, X-Ray Computed; D014182:Transplantation, Autologous
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins