title: Microfabricated magnetic sifter for high-throughput and high-gradient magnetic separation.
abstract: A microfabricated magnetic sifter has been designed and fabricated for applications in biological sample preparation. The device enables high-throughput, high-gradient magnetic separation of magnetic nanoparticles by utilizing columnar fluid flow through a dense array (~5000/mm(2)) of micropatterned slots in a magnetically soft membrane. The potential of the sifter for separation of magnetic nanoparticles conjugated with capture antibodies is demonstrated through quantitative separation experiments with CD138-labelled MACS nanoparticles. Capture efficiencies ranging from 28-37% and elution efficiencies greater than 73% were measured for a single pass through the sifter.
authors.affiliation: Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Earhart
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Wilson
authors.forename: Robert L
authors.initials: RL
authors.lastname: White
authors.forename: Nader
authors.initials: N
authors.lastname: Pourmand
authors.forename: Shan X
authors.initials: SX
authors.lastname: Wang