Accelerated solvent extraction for GC-based tobacco fingerprinting and its comparison with simultaneous distillation and extraction.
An accelerated solvent extraction (ASE) procedure has been developed as a pretreatment method for chemical fingerprinting of volatile and semi-volatile components in cut tobacco. The ASE extraction conditions including temperature, operation pressure and extraction cycles were optimized to maximize extraction yield. The method was validated with repeatability, recovery and linearity. Compared with simultaneous distillation extraction (SDE), ASE provides higher extraction yields, less extraction time, lower solvent consumption and less labor time, and is more suitable for tobacco sample preparation. A typical ASE extract was analyzed by gas chromatography/time-of-flight mass spectrometry (GC-TOFMS). A total of 305 components with signal-to-noise ratio higher than 100 were tentatively identified by NIST05 and Wiley database. Finally, 36 cigarette samples from six cigarette brands were analyzed using the developed chemical fingerprinting method. Partial least squares-discriminant analysis shows good discrimination of different cigarette brands. The results indicate that ASE method can serve as high-throughput sample preparation technique for cigarette chemical fingerprint analysis.
Li
Yong
Y
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Pang
Tao
T
Guo
Ziming
Z
Li
Yanli
Y
Wang
Xiaolin
X
Deng
Jianhua
J
Zhong
Kejun
K
Lu
Xin
X
Xu
Guowang
G
D002849:Chromatography, Gas; D016002:Discriminant Analysis; D057168:Distillation / Q000379:methods*; D016018:Least-Squares Analysis; D016014:Linear Models; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry*; D014026:Nicotiana / Q000737:chemistry*
D012997:Solvents