Effect of indirubin-3-monoxime against lung cancer as evaluated by histological and transmission electron microscopic studies.
The aim of this study is to evaluate the antitumor effect of indirubin-3-monoxime and its mode of action in benzo(α)pyrene [B(α)P] induced lung cancer in A/J mice. Light microscopic examination of lung sections of [B(α)P] induced lung cancer mice revealed the presence of adenocarcinoma characterized by extensive proliferation of alveolar epithelium and loss of alveolar spaces. The control lung tissue showed a normal architecture with clear alveolar spaces. Interestingly the indirubin-3-monoxime treated groups showed the reduced adenocarcinoma with appearance of alveolar spaces. Transmission Electron Microscopic (TEM) studies of lung sections of [B(α)P] induced lung cancer mice showed the presence of phaemorphic cells with dense granules and increased mitochondria. The lung sections of mice treated with indirubin-3-monoxime showed the presence of shrunken, fragmented, and condensed nuclei implying apoptosis. The effects were dose dependent and prominent in 10 mg/kg/5 d/week groups suggesting the therapeutic role of indirubin analogue against this deadly human malignancy. Here, our results indicate that indirubin-3-monoxime brings antitumor effect against [B(α)P] induced lung cancer by its apoptotic action in A/J mice.
Ravichandran
Kameswaran
K
Department of Zoology, Unit of Biomonitoring and Management, University of Madras, Guindy Campus, Chennai 600025, India.
Pal
Arttatrana
A
Ravichandran
Ramanibai
R
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001564:Benzo(a)pyrene / Q000633:toxicity; D002452:Cell Count; D002467:Cell Nucleus / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D003593:Cytoplasm / Q000187:drug effects; D006651:Histocytochemistry; D007211:Indoles / Q000494:pharmacology*; D008168:Lung / Q000033:anatomy & histology / Q000166:cytology / Q000473:pathology; D008175:Lung Neoplasms / Q000139:chemically induced / Q000473:pathology; D008297:Male; D051379:Mice; D046529:Microscopy, Electron, Transmission; D010091:Oximes / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects
D000970:Antineoplastic Agents; D007211:Indoles; D010091:Oximes; C474021:indirubin-3'-monoxime; D001564:Benzo(a)pyrene