Synthesis and evaluation of a new fluorescent transglycosylase substrate: lipid II-based molecule possessing a dansyl-C20 polyprenyl moiety.
The preparation of a novel fluorescent lipid II-based substrate for transglycosylases (TGases) is described. This substrate has characteristic structural features including a shorter lipid chain, a fluorophore tag at the end of the lipid chain rather than on the peptide chain, and no labeling with a radioactive atom. This fluorescent substrate is readily utilized in TGase activity assays to characterize TGases and also to evaluate the activities of TGase inhibitors.
Liu
Chen-Yu
CY
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Guo
Chih-Wei
CW
Chang
Yi-Fan
YF
Wang
Jen-Tsung
JT
Shih
Hao-Wei
HW
Hsu
Yu-Fang
YF
Chen
Chia-Wei
CW
Chen
Shao-Kang
SK
Wang
Yen-Chih
YC
Cheng
Ting-Jen R
TJ
Ma
Che
C
Wong
Chi-Huey
CH
Fang
Jim-Min
JM
Cheng
Wei-Chieh
WC
D001455:Bambermycins / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005453:Fluorescence; D016695:Glycosyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015394:Molecular Structure; D011106:Polyisoprenyl Phosphates / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014538:Uridine Diphosphate N-Acetylmuramic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
D004791:Enzyme Inhibitors; D011106:Polyisoprenyl Phosphates; D014538:Uridine Diphosphate N-Acetylmuramic Acid; C069249:muramyl-NAc-(pentapeptide)pyrophosphoryl-undecaprenol; D001455:Bambermycins; C437243:moenomycin A; D016695:Glycosyltransferases