title: The structure of the 5'-untranslated region of mammalian poly(A) polymerase-alpha mRNA suggests a mechanism of translational regulation.
abstract: Poly(A)polymerase-alpha (PAPOLA) has been the most extensively investigated mammalian polyadenylating enzyme, mainly in regard to its multifaceted post-translational regulation. The possibility of translational regulation of this enzyme was addressed. The transcription start site was mapped and two uORFs, highly conserved among several species, were identified in the 211-bp long, GC-rich, 5' UTR of the PAPOLA mRNA. Mutation of the 5' proximal AUG resulted in increased translational efficiency of the adjacent coding sequence, whereas no significant effect was observed after mutation of the second AUG. These observations imply that translational regulation is among the conserved mechanisms regulating PAPOLA expression.
authors.affiliation: Papanikolaou Research Center of Oncology, St Savvas Hospital, Athens, Greece.
authors.forename: Aikaterini
authors.initials: A
authors.lastname: Rapti
authors.forename: Theoni
authors.initials: T
authors.lastname: Trangas
authors.forename: Martina
authors.initials: M
authors.lastname: Samiotaki
authors.forename: Panayotis
authors.initials: P
authors.lastname: Ioannidis
authors.forename: Euthymios
authors.initials: E
authors.lastname: Dimitriadis
authors.forename: Christos
authors.initials: C
authors.lastname: Meristoudis
authors.forename: Stavroula
authors.initials: S
authors.lastname: Veletza
authors.forename: Nelly
authors.initials: N
authors.lastname: Courtis
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D002460:Cell Line; D017124:Conserved Sequence; D005091:Exons; D020862:GC Rich Sequence; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D011062:Polynucleotide Adenylyltransferase / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D020121:5' Untranslated Regions; D012333:RNA, Messenger; D011062:Polynucleotide Adenylyltransferase