DNA chips with conjugated polyelectrolytes in resonance energy transfer mode.
We show how to use well-defined conjugated polyelectrolytes (CPEs) combined with surface energy patterning to fabricate DNA chips utilizing fluorescence signal amplification. Cholesterol-modified DNA strands in complex with a CPE are adsorbed to a surface energy pattern, formed by printing with soft elastomer stamps. Hybridization of the surface bound DNA strands with a short complementary strand from solution is monitored using both fluorescence microscopy and imaging surface plasmon resonance. The CPEs act as antennas, enhancing resonance energy transfer to the dye-labeled DNA when complementary hybridization of the double strand occurs.
Wigenius
Jens A
JA
Biomolecular and Organic Electronics, Department of Physics, Chemistry and Biology (IFM), Linköping University, SE-581 83 Linköping, Sweden. jensa@ifm.liu.se
Magnusson
Karin
K
Björk
Per
P
Andersson
Olof
O
Inganäs
Olle
O
D000085:Acetates / Q000378:metabolism; D000327:Adsorption; D001483:Base Sequence; D002232:Carbocyanines / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D002867:Chromones / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020349:Surface Plasmon Resonance
D000085:Acetates; C400304:Alexa 350; D002232:Carbocyanines; D002867:Chromones; D004277:DNA, Single-Stranded; D004129:Dimethylpolysiloxanes; D004573:Electrolytes; C085321:cyanine dye 5; C013830:baysilon; D002784:Cholesterol