title: Quantification of nortriptyline in plasma by HPLC and fluorescence detection.
abstract: A simple, sensitive and specific high-performance liquid chromatography method has been developed for the determination of nortriptyline (NT) in plasma samples. The assay involved derivatization with 9H-fluoren-9-ylmethyl chloroformate (Fmoc-Cl) and isocratic reversed-phase (C(18)) chromatography with fluorescence detection. The developed method required only 100 microl of plasma sample, deproteinized and derivatized in one step. Calibration curves were lineal over the concentration range of 5-5000 ng/ml. The derivatization reaction was performed at room temperature in 20 min and the obtained NT derivative was stable for at least 48 h at room temperature. The within-day and between-day relative standard deviation was below 8%. The limit of detection (LOD) was 2 ng/ml, and the lower limit of quantification (LLOQ) was established at 10 ng/ml. The method was applied on plasma collected from rats, at different time intervals, after intravenous administration of 0.5 mg of NT.
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Valencia, Avda. V. Andrés Estellés, s/n, 46100-Burjassot, Valencia, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Almudever
authors.forename: José-Esteban
authors.initials: JE
authors.lastname: Peris
authors.forename: Teresa
authors.initials: T
authors.lastname: Garrigues
authors.forename: Octavio
authors.initials: O
authors.lastname: Diez
authors.forename: Ana
authors.initials: A
authors.lastname: Melero
authors.forename: Manuel
authors.initials: M
authors.lastname: Alós
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D009661:Nortriptyline / Q000097:blood*; D051381:Rats
chemical_list: D000928:Antidepressive Agents; D009661:Nortriptyline