title: Highly sensitive non-stripping gold nanoparticles-based chemiluminescent detection of DNA hybridization coupled to magnetic beads.
abstract: We have developed a new nanoparticle (NP)-based chemiluminescent (CL) method for the sensitive detection of DNA hybridization. The "sandwich-type" DNA technique comprises NH(2)-functionalized capture DNA immobilized on magnetic beads that can hybridize with one end of the target DNA. The other end of the target is recognized by a reporter DNA probe labeled with Au NPs on its 3-terminus. The hybridization events were monitored by a simple and direct luminol-AgNO(3) CL reaction with a detection limit of 0.5 pM through a non-stripping Au NPs catalysis. Moreover, the CL reaction of non-stripping Au NPs-luminol-AgNO(3) offers the advantages of being simpler and faster as compared to previous stripping Ag NPs and CuS methods. This simple and easy-to-use technique holds great promise for clinical applications of DNA hybridization.
authors.affiliation: School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai, 201203, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Cai
authors.forename: Liang
authors.initials: L
authors.lastname: Xin
authors.forename: Choiwan
authors.initials: C
authors.lastname: Lau
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Lu
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012835:Silver Nitrate / Q000737:chemistry
chemical_list: D015342:DNA Probes; D008165:Luminol; D006046:Gold; D004247:DNA; D012835:Silver Nitrate