Evanescent wave-initiated photopolymerisation as a new way to create monolithic open-tubular capillary columns: use as enzymatic microreactor for on-line protein digestion.
Evanescent wave-initiated photopolymerisation in an optically wave guiding PTFE-coated fused silica capillary using light-emitting diode as a light source, is established here as a way to fabricate monolithic porous layer open-tubular capillary columns with a potential in capillary separation methods; application of the obtained open-tubular columns as enzymatic microreactors for on-line protein digestion is demonstrated.
Abele
Silvija
S
Dublin City University, Irish Separation Science Cluster and School of Chemical Sciences, Glasnevin, Dublin 9, Ireland.
Smejkal
Petr
P
Yavorska
Oksana
O
Foret
Frantisek
F
Macka
Mirek
M
D000818:Animals; D019149:Bioreactors; D053801:Capillary Electrochromatography; D004798:Enzymes / Q000378:metabolism*; D006736:Horses; D013058:Mass Spectrometry / Q000379:methods*; D009211:Myoglobin / Q000737:chemistry / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D016062:Porosity; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry; D014466:Ultraviolet Rays
D004798:Enzymes; D009211:Myoglobin; D011506:Proteins; D012822:Silicon Dioxide; D011138:Polytetrafluoroethylene; D010434:Pepsin A