Differential apoptotic pathways activated in response to Cu-induced or HOCl-induced LDL oxidation in U937 monocytic cell line.
We compared the apoptotic mechanism involved in U937 human monocytic cell line in presence of oxidized low-density lipoproteins (oxLDL) obtained after treatment with hypochlorous acid (HOCl) or copper (Cu). Both types of oxLDL induced U937 apoptotic cell death via the mitochondrial pathway. In contrast to HOCl-oxLDL, Cu-oxLDL induced apoptosis via a caspase-independent mechanism, with no activation of pro-caspase-3, but via the release of apoptosis inducing factor (AIF) from mitochondria. The apoptotic program of the monocyte differs depending on the mode of LDL oxidation, based on differences in the oxidatively modified components of the two oxLDL types.
Ermak
Natalia
N
Laboratory of Biochemistry A, Necker Hospital, Paris, France. natalia.ermak@nck.aphp.fr
Lacour
Bernard
B
Goirand
Françoise
F
Drüeke
Tilman B
TB
Vicca
Stéphanie
S
D017209:Apoptosis; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D003300:Copper / Q000494:pharmacology; D006801:Humans; D006997:Hypochlorous Acid / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000378:metabolism* / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010084:Oxidation-Reduction; D020298:U937 Cells
D051033:Apoptosis Inducing Factor; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D006997:Hypochlorous Acid; D003300:Copper