Binding and neutralization characteristics of a panel of monoclonal antibodies to human papillomavirus 58.
Human papillomavirus (HPV) 58 is a high-risk HPV type associated with progression to invasive genital carcinomas. We developed six monoclonal antibodies (mAbs) against HPV58 L1 virus-like particles that bind conformational epitopes on HPV58. The hybridoma cell lines were adapted to serum- and animal component-free conditions and the mAb supernatants were affinity-purified. The six mAbs neutralized HPV58 pseudoviruses (PsVs) and 'quasivirions' with different capacities. The mAbs differed in their ability to prevent PsV58 attachment to HaCaT cells, to the extracellular matrix (ECM) deposited by HaCaT cells, to heparin and to purified human laminin 5, a protein in the ECM. These mAbs provide a unique set of tools to study the binding properties of a previously untested, high-risk HPV type and the opportunity to compare these characteristics with the binding of other HPV types.
Brendle
Sarah A
SA
The Jake Gittlen Cancer Research Foundation and Department of Pathology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
Culp
Timothy D
TD
Broutian
Tatevik R
TR
Christensen
Neil D
ND
D052685:Alphapapillomavirus / Q000145:classification* / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000915:Antibody Affinity / Q000276:immunology*; D000918:Antibody Specificity; D002460:Cell Line; D000939:Epitopes; D006801:Humans; D007074:Immunoglobulin G
D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000939:Epitopes; D007074:Immunoglobulin G