title: Prevalence and antibiotic resistance pattern of metallo-beta-lactamase-producing Pseudomonas aeruginosa from burn patients--experience of an Indian tertiary care hospital.
abstract: The objective of the study is to determine the prevalence of Pseudomonas aeruginosa from burn patients, antibiotic resistance pattern and occurrence of acquired MBL-producing P. aeruginosa among isolates collected from burn patients. In this study, two phenotypic methods were used for the detection of MBL-producing P. aeruginosa: zone enhancement with EDTA-impregnated imipenem and ceftazidime discs, and modified Hodge test. One hundred fifty-four isolates of P. aeruginosa were obtained from July 2007 to July 2008. Infection was increased up to 95% in hospitalized patients for >50 days. Highest infection of 39% was found in patients, who had 41 to 50% of burn area followed by 19% in patients with 31 to 40% of burn area. The most common bacteria isolated were P. aeruginosa (55.0%), followed by Staphylococcus aureus (19.29%) and Klebsiella spp. (11.43%). Sixteen percent isolates of P. aeruginosa were positive for metallo-beta-lactamase production by both methods. Antibiotic resistance pattern of MBL-positive strains showed the highest resistance to ceftazidime (70%) followed by chloramphenicol (68%) and gentamicin (62.5%). Routine detection of MBLs ensuring optimal patient care and careful in vitro testing before antibiotic use may help in the prevention and treatment of burn patients infected with metallo-beta- lactamase-producing P. aeruginosa.
authors.affiliation: Department of Microbiology, Chhatrapati Shahuji Maharaj Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Rajput
authors.forename: Rishi
authors.initials: R
authors.lastname: Saxena
authors.forename: Kaleshwar Prasad
authors.initials: KP
authors.lastname: Singh
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
authors.forename: Shubhra
authors.initials: S
authors.lastname: Singh
authors.forename: Ashish
authors.initials: A
authors.lastname: Gupta
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Singh
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002056:Burns / Q000382:microbiology*; D003428:Cross Infection / Q000188:drug therapy / Q000201:enzymology / Q000453:epidemiology / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D015995:Prevalence; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000201:enzymology* / Q000453:epidemiology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents