An induction effect of heat shock on the transcript of globular acetylcholinesterase in NG108-15 cells.
Heat shock response, an induced transcription of a set of genes in response to high temperature, occurs in all organisms. In neurons, the catalytic subunit of acetylcholinesterase (AChE(T)) interacts with proline-rich membrane anchor (PRiMA) to form a globular tetrameric form (G(4) form). In this study, we examined the effects of heat shock on the transcription and protein assembly of AChE(T) in cultured NG108-15 cells. The transcription of AChE(T) was rapidly induced by heat shock at 40 degrees C, reaching a 15-fold increase in 3h and decreasing thereafter. On the other hand, the level of PRiMA mRNA was not affected after the heat shock. In parallel with AChE(T) mRNA, the enzymatic activity of cellular AChE, in terms of G(1) and G(2) forms, was increased after heat shock; however, the PRiMA-linked G(4) remained unchanged. These results suggest that heat shock can induce the expression level of AChE(T) by the regulation of AChE(T) transcripts in NG108-15 cells.
Chen
Vicky P
VP
Department of Biology and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Kowloon, Hong Kong SAR, China. pingchen@ust.hk
Xie
Heidi Q
HQ
Chan
Wallace K B
WK
Leung
K Wing
KW
Choi
Roy C Y
RC
Tsim
Karl W K
KW
D000110:Acetylcholinesterase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D055162:Biocatalysis; D045744:Cell Line, Tumor; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015533:Transcriptional Activation
D008565:Membrane Proteins; D021122:Protein Subunits; D012333:RNA, Messenger; D000110:Acetylcholinesterase