title: Up-regulation of the Apo/Fas (CD95) complex on neutrophils harvested during cardiac surgery: distinct findings in patients operated on with or without the use of cardiopulmonary bypass.
abstract: OBJECTIVE
  In a group of patients undergoing cardiac surgery performed both with ("on-pump") and without the use ("offpump") of cardiopulmonary bypass (CPB), we studied the changes of neutrophil membrane apoptosis-inducing complex Apo/Fas.
  
  
  METHODS
  Expression of Apo/Fas (CD95) on leukocytes was evaluated by flow cytometry.
  
  
  RESULTS
  In "on-pump" patients, we found an increase in the expression of CD95 median intensity fluorescence (MFI) on granulocytes from a baseline level median=56, (Q( 1)=45.5, Q(3)=64) to a median=88, (Q(1)=62, Q( 3)=109.5; p<0.01) at the 3(rd) postoperative day and median=74, (Q(1)=63, Q(3)=84.5; p<0.01) at the 7(th) postoperative day. In "off-pump" patients, granulocyte CD95 MFI was median=55, (Q(1)=51, Q(3)=84) before surgery. The significant increase was found on the 3(rd) postoperative day only; median=90, (Q( 1)=66; Q(3)=98; p<0.05). A similar pattern in the CD95 expression was also found if percentage changes of granulocyte CD95 MFI were followed. Moreover, the significantly increased Apo/Fas expression expressed as a percentage change of CD95 MFI was found in "on-pump" patients compared to "off-pump" patients, both at the 3(rd) postoperative day (p<0.05) and at the 7(th) postoperative day (p<0.01).
  
  
  CONCLUSIONS
  This is the first direct evidence of increasing densities of the Apo/Fas complex on neutrophils in cardiac surgical patients.
authors.affiliation: Department of Cardiac Surgery, Charles University in Prague, University Hospital and Medical Faculty in Hradec Kralove, Hradec Kralove, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kunes
authors.forename: Jiri
authors.initials: J
authors.lastname: Mandak
authors.forename: Jan
authors.initials: J
authors.lastname: Harrer
authors.forename: Martina
authors.initials: M
authors.lastname: Kolackova
authors.forename: Ctirad
authors.initials: C
authors.lastname: Andrys
authors.forename: Monika
authors.initials: M
authors.lastname: Holicka
authors.forename: Jan
authors.initials: J
authors.lastname: Krejsek
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000276:immunology; D006348:Cardiac Surgical Procedures; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D011183:Postoperative Complications / Q000276:immunology; D015854:Up-Regulation / Q000276:immunology; D019014:fas Receptor / Q000378:metabolism*
chemical_list: C491901:FAS protein, human; D019014:fas Receptor