Efficient transposition of a single Minos transposon copy in the genome of the ascidian Ciona intestinalis with a transgenic line expressing transposase in eggs.
Transgenesis with transposons is an important technique for studying genetic functions. In the ascidian Ciona intestinalis, methods for germline transformation with the Tc1/mariner transposon Minos have been established. A system to remobilize a single Minos copy in the genome is needed to refine this transgenic technique. In this study, such an experimental system was established with a transgenic line expressing Minos transposase in eggs. In the eggs of a double transgenic animal from a cross between the egg transposase line and a transgenic line having a single Minos insertion, the transposon was transposed into new positions of the Ciona genome, thus creating new insertions. Some of the new insertions caused enhancer detection. The majority of the new insertion sites were mapped on different chromosomes from that of the transposon donor. This characteristic of Minos is in contrast to that of the Sleeping Beauty transposon, which causes frequent intrachromosomal transposition.
Hozumi
Akiko
A
Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka 415-0025, Japan.
Kawai
Narudo
N
Yoshida
Reiko
R
Ogura
Yosuke
Y
Ohta
Naoyuki
N
Satake
Honoo
H
Satoh
Nori
N
Sasakura
Yasunori
Y
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002938:Ciona intestinalis / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics*; D004526:Efficiency; D004625:Embryo, Nonmammalian; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental; D016678:Genome; D008954:Models, Biological; D016254:Mutagenesis, Insertional / Q000235:genetics / Q000502:physiology; D010063:Ovum / Q000201:enzymology / Q000378:metabolism*; D019895:Transposases / Q000235:genetics / Q000378:metabolism*; D014561:Urochordata / Q000235:genetics / Q000378:metabolism
D004251:DNA Transposable Elements; C473499:Minos transposase; D019895:Transposases