[Genetic diversity of different geographical populations of Oxya chinensis based on AFLP analysis].
In order to study the genetic diversity and genetic structure of populations, 7 populations of Oxya chinensis from 7 provinces (or cities) of China were analyzed using AFLP technique. A total of 336 reproducible bands were amplified with 7 primer combinations from 128 individuals. Two hundred and ninety-two bands (86.90%) were polymorphic. High genetic diversity was found among O. chinensis populations and Wanning population had higher genetic diversity than other populations. Mantel test (r=0.27, P=0.89) suggested that there was no significant association between genetic distance and geographic distance. Remarkable genetic differentiation was found among populations. Unweighted pair group method average (UPGMA) tree showed that the 7 O. chinensis populations were divided into 3 groups: Changping of Beijing, Tai-yuan of Shanxi and Jining of Shandong populations in the north; Hanzhong of Shaanxi, Changsha of Hunan and Laibin of Guangxi populations in the south; and Wanning of Hainan population. Principal component analysis indicated significant genetic differentiation of the north and the south populations and island and continent populations existed in the 7 O. chinensis populations because of geographic isolation.
Ma
Jin
J
School of Life Science and Technology, Shanxi University, Taiyuan 030006, China. mjoyxx@sina.com
Li
Tao
T
Long
Wen-Min
WM
An
Wei-Wei
WW
Guo
Ya-Ping
YP
Ma
En-Bo
EB
D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D002681:China; D014644:Genetic Variation; D006110:Grasshoppers / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic