Self-reference quantitative phase microscopy for microfluidic devices.
This Letter describes a quantitative phase microscopy for microfluidic devices using a simple self-referencing interferometry. Compared with the gross dimensions of the microfluidic device, the microchannel occupies only a small area of the device. Hence, the reference field can be generated by inverting the relative position of the specimen and background. Our system is realized using an extended depth-of-field optics in the form of Michelson interferometry, which allows quantitative phase measurement for an increased depth-of-field without moving objective lens or specimen. Furthermore, the system can be readily converted to a higher signal-to-noise ratio Hilbert phase microscopy thanks to the simultaneous acquisition of double interferograms. The performance of our system is verified using polymer beads, micropatterning poly(dimethylsiloxane) (PDMS), and embryo cells in the microchannels.
Jang
Jaeduck
J
Bio Imaging & Signal Processing Laboratory, Department of Bio and Brain Engineering,Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
Bae
Chae Yun
CY
Park
Je-Kyun
JK
Ye
Jong Chul
JC
D000818:Animals; D002417:Cattle; D004622:Embryo, Mammalian / Q000378:metabolism; D007368:Interferometry; D008027:Light; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*