Viral shedding after p53 adenoviral gene therapy in 10 cases of esophageal cancer.
We detected adenoviral DNA fragments in excretions of 10 esophageal cancer patients by DNA-PCR after tumor injection of Ad-CMV-vector. A total of 220 samples consisting of feces, gargling saliva, urine, and blood plasma were assessed. A total of 29.7% of feces samples and 13.2% of gargling saliva samples were positive for adenoviral DNA fragments, but 89.7% of the positive feces samples and all of the positive gargling saliva samples turned negative on day 12 after tumor injection. Although adenoviral DNA fragments may be pathogen-free, patients' feces and gargling saliva contain adenoviral DNA fragments for 12 days after injection.
Kawahira
Hiroshi
H
Department of Frontier Surgery, Chiba University Graduate School of Medicine, Chuoh-ku, Chiba, Japan.
Matsushita
Kazuyuki
K
Shiratori
Tooru
T
Shimizu
Takanori
T
Nabeya
Yoshihiro
Y
Hayashi
Hideki
H
Ochiai
Takenori
T
Matsubara
Hisahiro
H
Shimada
Hideaki
H
D000256:Adenoviridae / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000628:therapy* / Q000821:virology*; D016158:Genes, p53; D015316:Genetic Therapy; D006801:Humans; D016133:Polymerase Chain Reaction; D017201:Virus Shedding