title: Beneficial effects of dietary restriction in type 2 diabetic rats: the role of adipokines on inflammation and insulin resistance.
abstract: Inflammation plays an important role in diabetes mellitus and its complications. In this context, the negative cross-talk between adipose tissue and skeletal muscle leads to disturbances in muscle cell insulin signalling and induces insulin resistance. Because several studies have shown that energy restriction brings some benefits to diabetes, the aim of the present study was to evaluate the effects of dietary restriction on systemic and skeletal muscle inflammatory biomarkers, such C-reactive protein, adipokines and cytokines, and in insulin resistance in Goto-Kakizaki rats. This is an animal model of spontaneous non-obese type 2 diabetes with strongly insulin resistance and without dyslipidaemia. Animals were maintained during 2 months of dietary restriction (50 %) and were killed at 6 months of age. Some biochemical determinations were done using ELISA and Western blot. Data from the present study demonstrate that in Goto-Kakizaki rats the dietary restriction improved insulin resistance, NEFA levels and adipokine profile and ameliorated inflammatory cytokines in skeletal muscle. These results indicate that dietary restriction in type 2 diabetes enhances adipose tissue metabolism leading to an improved skeletal muscle insulin sensitivity.
authors.affiliation: Faculty of Medicine, Institute of Physiology, University of Coimbra, Coimbra, Portugal. joanacrisostomo@hotmail.com
authors.forename: Joana
authors.initials: J
authors.lastname: Crisóstomo
authors.forename: Lisa
authors.initials: L
authors.lastname: Rodrigues
authors.forename: Paulo
authors.initials: P
authors.lastname: Matafome
authors.forename: Carmen
authors.initials: C
authors.lastname: Amaral
authors.forename: Elsa
authors.initials: E
authors.lastname: Nunes
authors.forename: Teresa
authors.initials: T
authors.lastname: Louro
authors.forename: Pedro
authors.initials: P
authors.lastname: Monteiro
authors.forename: Raquel
authors.initials: R
authors.lastname: Seiça
mesh_terms: D054392:Adipokines / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D031204:Caloric Restriction; D016207:Cytokines / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000178:diet therapy* / Q000378:metabolism; D002149:Energy Intake; D004797:Enzyme-Linked Immunosorbent Assay; D005227:Fatty Acids / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000378:metabolism; D007333:Insulin Resistance; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D054392:Adipokines; D015415:Biomarkers; D016207:Cytokines; D005227:Fatty Acids; D018836:Inflammation Mediators