The dependence of enhanced lysosomal activity on the cellular aging of bovine aortic endothelial cells.
Lysosomes are a cell organelle type processing antimicrobial activity. Here, we investigate the lysosomal activity in a primary cell line, bovine aortic endothelial cells (BAECs), during cellular aging, based on the antimicrobial activity of lysosomes isolated from BAECs at cell passages 4, 6, 8, and 10. Cytochemical analysis of lysosomes with LysoTracker reagent revealed the number of lysosome-like organelles surrounding the nucleus initially increased drastically in the BAECs and continued increasing gradually until passage 10. The lysosomes isolated from each successive passage of BAECs exhibited increased antimicrobial activity against Escherichia coli, and, in addition, an age-dependent increase in lysosome intensity coincided with increased lysosomal antimicrobial activity.
Yoon
Jihee
J
Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561-756, South Korea.
Kim
Ki-Ju
KJ
Choi
Young-Woo
YW
Shin
Hwa Sung
HS
Kim
Yang-Hoon
YH
Min
Jiho
J
D000588:Amines; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000201:enzymology; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence; D015169:Colony Count, Microbial; D042783:Endothelial Cells / Q000201:enzymology*; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D005456:Fluorescent Dyes; D008247:Lysosomes / Q000201:enzymology*; D018613:Microscopy, Confocal; D013997:Time Factors
D000588:Amines; D005456:Fluorescent Dyes; C493330:Red DND-99