title: Activity of an enzyme immobilized on superparamagnetic particles in a rotational magnetic field.
abstract: We immobilize alpha-amylase extracted from Bacillus Iicheniformis on the surfaces of superparamagnetic particles and investigate the effect of a rotational magnetic field on the enzyme's activity. We find that the activity of the enzyme molecules immobilized on superparamagnetic particles increases in the rotational magnetic field and reaches maximum at a certain frequency. We clarify the effect of the cluster structures formed by the superparamagnetic particles on the activity. Enzyme reactions are enhanced even in a tiny volume of solution using the present method, which is very important for the development of efficient micro reactors and micro total analysis systems (mu-TAS).
authors.affiliation: Bio-Nano Electronics Research Centre, Toyo University, Saitama 350-8585, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Mizuki
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Watanabe
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nagaoka
authors.forename: Tadamasa
authors.initials: T
authors.lastname: Fukushima
authors.forename: Hisao
authors.initials: H
authors.lastname: Morimoto
authors.forename: Ron
authors.initials: R
authors.lastname: Usami
authors.forename: Toru
authors.initials: T
authors.lastname: Maekawa
mesh_terms: D004574:Electromagnetic Fields; D004800:Enzymes, Immobilized / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011487:Protein Conformation; D012399:Rotation; D000516:alpha-Amylases / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D000516:alpha-Amylases; C501919:alpha-amylase, Bacillus licheniformis; D052203:Ferrosoferric Oxide