Secretion profile of human bone marrow stromal cells: donor variability and response to inflammatory stimuli.
Mesenchymal stem cells (MSC) derived from bone marrow are ideal transplants for a variety of CNS disorders and appear to support recovery after injury by secreting therapeutic factors. There is considerable variability in the secretion profile of MSC derived from different donors and it is known that MSC secretion changes in response to inflammatory stimuli, but no comprehensive analysis has been performed to address these issues. Here we show that MSC from seven donors secrete chemokines and cytokines in variable ranges, with some factors showing high variability. Treatment of cultured MSC with pro-inflammatory cytokines or tissue extracts from injured spinal cord resulted in up-regulation of selected cytokines, whereas treatment with an anti-inflammatory cytokine had little effect, indicating that the secretion profile is tightly regulated by environmental challenges. Patterns of up-regulated cytokines were similar in MSC from different donors suggesting a comparable response to inflammatory stimuli.
Zhukareva
Victoria
V
Department of Neurobiology & Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA.
Obrocka
Maria
M
Houle
John D
JD
Fischer
Itzhak
I
Neuhuber
Birgit
B
D000293:Adolescent; D000328:Adult; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008875:Middle Aged; D051381:Rats; D013119:Spinal Cord Injuries / Q000473:pathology; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism; D014019:Tissue Donors; D014020:Tissue Extracts; D055815:Young Adult
D016207:Cytokines; D014020:Tissue Extracts