title: Effect of a novel topical diester glucocorticoid spray on immediate- and late-phase cutaneous allergic reactions in Maltese-beagle atopic dogs: a placebo-controlled study.
abstract: The inhibitory effect of 0.0584% hydrocortisone aceponate spray on immediate- and late-phase skin reactions and the duration of inhibition after medication withdrawal were studied in 10 Maltese-beagle atopic dogs. All subjects were sprayed on axillary and inguinal regions and on one randomly chosen side of the thorax once daily for 14 (phase 1) or 7 days (phase 2). Intradermal injections (IDT) of histamine and anticanine IgE antiserum were performed bilaterally on the thorax before, 7 and 14 days after treatment. During phase 2, IDT was performed once weekly for 5 weeks. Each IDT was evaluated by an investigator blinded to the site of active treatment. Skin biopsies of 24-h anti-IgE-associated late-phase reactions were collected from both thoracic sides before and 14 days after treatment to determine the number of inflammatory cells and dermal thickness. Phase 1: Histamine and anti-IgE-induced global wheal scores at treated sites were significantly lower after 7 and 14 days with negative reactions present in >90% of dogs. Late-phase reactions at both sides were also significantly decreased compared with that at baseline, and this was associated with reduced inflammatory cell influx. Moreover, a significant decrease in dermal thickness was recorded at treated sides after 14 days. Phase 2: Histamine reactions became positive at untreated sides in all dogs 2 weeks after treatment. In conclusion, the 0.0584% hydrocortisone aceponate spray significantly decreased immediate- and late-phase IDT reactions, and prolonged application caused skin atrophy at treated sites. A 2-week withdrawal period prior to IDT is proposed.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA.
authors.forename: Petra
authors.initials: P
authors.lastname: Bizikova
authors.forename: Keith E
authors.initials: KE
authors.lastname: Linder
authors.forename: Judy
authors.initials: J
authors.lastname: Paps
authors.forename: Thierry
authors.initials: T
authors.lastname: Olivry
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000627:therapeutic use; D003876:Dermatitis, Atopic / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D005260:Female; D006632:Histamine / Q000009:adverse effects; D006854:Hydrocortisone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000188:drug therapy / Q000662:veterinary*; D006969:Hypersensitivity, Immediate / Q000139:chemically induced / Q000188:drug therapy / Q000662:veterinary*; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary; D007428:Intradermal Tests; D008297:Male; D012867:Skin / Q000473:pathology; D012882:Skin Tests
chemical_list: D000893:Anti-Inflammatory Agents; C063919:hydrocortisone aceponate; D006632:Histamine; D006854:Hydrocortisone