title: Foodproof Salmonella Detection Kit. Performance Tested Method 120301.
abstract: The foodproof Salmonella Detection Kit was previously validated in the Performance Tested Methods program for the detection of Salmonella species in a variety of foods, including milk powder, egg powder, coconut, cocoa powder, chicken breast, minced meat, sliced sausage, sausage, smoked fish, pasta, white pepper, cumin, dough, wet pet food, dry pet food, ice cream, watermelon, sliced cabbage, food dye, and milk chocolate. The method was shown to be equivalent to the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA-BAM) and the U.S. Department of Agriculture, Food Safety and Inspection Service's Microbiology Laboratory Guidebook reference culture procedures. In the first Emergency Response Validation (ERV) extension study, peanut butter was inoculated with S. enterica. ser Typhimurium. For the low inoculation level (1.08 CFU/25 g), a Chi-square value of 2.25 indicated that there was no significant performance difference between the foodproof Salmonella Detection Kit and the FDA-BAM reference method. For high-level inoculation (11.5 CFU/25 g) and uninoculated control, there was 100% agreement between the methods. In the second ERV extension study, peanut butter was inoculated with S. enterica. ser Typhimurium. For both inoculation levels (0.1 and 0.5 CFU/25 g by most probable number), Chi-square values of 0 indicated that there was no significant performance difference between foodproof Salmonella Detection Kit and the FDA-BAM reference method.
authors.affiliation: Merck KGaA, Frankfurter Strasse 250, 64293 Darmstadt, Germany.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Lindhardt
authors.forename: Holger
authors.initials: H
authors.lastname: Schönenbrücher
authors.forename: Jörg
authors.initials: J
authors.lastname: Slaghuis
authors.forename: Andreas
authors.initials: A
authors.lastname: Bubert
authors.forename: Rolf
authors.initials: R
authors.lastname: Ossmer
authors.forename: Benjamin
authors.initials: B
authors.lastname: Junge
authors.forename: Kornelia
authors.initials: K
authors.lastname: Berghof-Jäger
mesh_terms: D003470:Culture Media; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012475:Salmonella / Q000737:chemistry* / Q000235:genetics; D019779:Salmonella enterica / Q000737:chemistry / Q000235:genetics; D012996:Solutions; D013696:Temperature
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D012996:Solutions