title: Lactobacillus hayakitensis, L. equigenerosi and L. equi, predominant lactobacilli in the intestinal flora of healthy thoroughbreds.
abstract: To detect the predominant lactobacilli in the intestinal flora of healthy thoroughbreds, we isolated lactobacilli from the feces of nine thoroughbreds (five males and four females; 0-15-year-old). The isolated lactobacilli comprise 17 species (37 strains), and they were classified into five groups: Lactobacillus salivarius (6 species), L. reuteri (6 species), Lactobacillus delbrueckii (3 species), L. buchneri (1 species) and L. vitulinus (1 species). On the basis of 16S rRNA gene sequences, we identified 3 other phylogenetic relatives belonging to the genus Lactobacillus. These results suggest that the intestinal flora of thoroughbreds may comprise many species of the genus Lactobacillus. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analyses of the 340-bp fragments of the 16S rRNA genes from the same nine fecal samples showed that L. hayakitensis, L. equigenerosi and L. equi are contained in all the samples, suggesting that these species are predominant lactobacilli in the intestinal flora of thoroughbreds.
authors.affiliation: School of Veterinary Medicine, Azabu University, Sagamihara, Japan. morita@azabu-u.ac.jp
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Morita
authors.forename: Akiyo
authors.initials: A
authors.lastname: Nakano
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Shimazu
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Toh
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Nakajima
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nagayama
authors.forename: Shin
authors.initials: S
authors.lastname: Hisamatsu
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kato
authors.forename: Misako
authors.initials: M
authors.lastname: Takagi
authors.forename: Hideto
authors.initials: H
authors.lastname: Takami
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Akita
authors.forename: Minoru
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Toshio
authors.initials: T
authors.lastname: Masaoka
authors.forename: Masaru
authors.initials: M
authors.lastname: Murakami
mesh_terms: D000818:Animals; D005260:Female; D006736:Horses / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000302:isolation & purification*; D008297:Male