New anti-inflammatory sterols from the Red Sea sponges Scalarispongia aqabaensis and Callyspongia siphonella.
Bioassay-guided fractionation of the anti-inflammation fractions of the Red Sea sponges Scalarispongia aqabaensis and Callyspongia siphonella yielded two new sterols from chloroform fractions of methanol extracts, namely scalaristerol (5alpha,8alpha-dihydroxycholest-6-en-3beta-ol) (1) from Scalarispongia aqabaensis, and callysterol (ergosta-5,11-dien-3beta-ol) (2) from Callyspongia siphonella. Structure determination was based on extensive NMR studies and mass spectrometry. The antiinflammatory activity of compounds 1 and 2 was assessed using the rat-hind paw edema method and by study of their effect on the release of O2(-) and TXB2 from LPS-activated rat neonatal microglia.
Youssef
Diaa T A
DT
Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt. diaa22@yahoo.com
Ibrahim
Amany K
AK
Khalifa
Sherief I
SI
Mesbah
Mostafa K
MK
Mayer
Alejandro M S
AM
van Soest
Rob W M
RW
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis* / Q000494:pharmacology / Q000627:therapeutic use; D047128:Callyspongia / Q000737:chemistry*; D004487:Edema / Q000188:drug therapy; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D010840:Phytosterols / Q000302:isolation & purification*; D051381:Rats; D013481:Superoxides / Q000378:metabolism; D013929:Thromboxane B2 / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D010840:Phytosterols; D013481:Superoxides; D013929:Thromboxane B2