Bone marrow-derived mesenchymal stem cells undergo JCV T-antigen mediated transformation and generate tumors with neuroectodermal characteristics.
There is now accumulating evidence showing that some tumors may arise from transformed stem cells. In this study we demonstrate that adult bone marrow- derived mesenchymal stem cells (MSCs) undergo neoplastic transformation induced by the human polyomavirus JCV, early protein, T-antigen, and are tumorigenic when transplanted into the flanks of Nude mice as compared to non-transformed MSCs. Histologically, the tumors are heterogeneous with mesenchymal and neural crest characteristics as evidenced by expression of the neural crest markers p75, SOX-10, and S-100, with populations of tumor cells exhibiting characteristics of primitive neuroectodermal cells. In addition, a subset of T-antigen positive tumor cells exhibit a high proliferation index as detected by Ki-67 labeling, and co-express CD133, a marker which is expressed on cancer stem cells. These results show that tumors with neuroectodermal characteristics may arise from transformation of MSCs, a globally accessible adult stem cell with multipotent differentiation capacity. In light of earlier reports on the association of JCV with a broad variety of human tumors, our data suggests that T-antigen transformation of adult stem cells with a multipotent capacity can serve as a possible common origin for some of these cancers, and offers a novel model for oncogenesis.
Del Valle
Luis
L
Departments of Neuroscience and Pathology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Piña-Oviedo
Sergio
S
Department of Neuroscience, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Perez-Liz
Georgina
G
Department of Neuroscience, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Augelli
Brian J
BJ
Department of Neurology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Azizi
S Ausim
SA
Department of Neurology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Khalili
Kamel
K
Temple University School of Medicine; Philadelphia, PA.
Gordon
Jennifer
J
Department of Neuroscience, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Krynska
Barbara
B
Department of Neurology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
D053687:Adult Stem Cells / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007577:JC Virus / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D017599:Neuroectodermal Tumors / Q000378:metabolism / Q000473:pathology*; D051381:Rats
D000957:Antigens, Viral, Tumor