title: [Microwave radiation decreases the expressions of occludin and JAM-1 in rats].
abstract: OBJECTIVE
  To explore the changes in the expressions of the tight junction related protein occludin and junctional adhesion molecule-1 (JAM-1) of the blood-testis barrier and their significance in rats after microwave radiation.
  
  
  METHODS
  Eighty male Wistar rats were exposed to microwave radiation with average power density of 0, 10, 30 and 100 mW/cm2 for five minutes, and dynamic changes in the expressions of testicular occludin and JAM-1 were observed by Western blot and image analysis at 6 h, 1 d, 3 d, 7 d and 14 d after the radiation.
  
  
  RESULTS
  There was a significant down-regulation in the expression of the occludin protein at 3 - 7 d, 6 h - 7 d and 6 h - 14 d (P < 0. 05), as well as in that of JAM-1 at 3 - 7 d, 1 - 7 d and 1-14 d (P < 0.05) after exposure to 10, 30 and 100 mW/cm2 microwave radiation.
  
  
  CONCLUSIONS
  The decreased protein expressions of occludin and JAM-1 may play an important role in the microwave radiation induced-damage to the blood-testis barrier.
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Gao
authors.forename: Shui-Ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Rui-Yun
authors.initials: RY
authors.lastname: Peng
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zuo
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Wang
authors.forename: Ya-Bing
authors.initials: YB
authors.lastname: Gao
authors.forename: Ji
authors.initials: J
authors.lastname: Dong
authors.forename: Zhen-Tao
authors.initials: ZT
authors.lastname: Su
mesh_terms: D000818:Animals; D001814:Blood-Testis Barrier / Q000378:metabolism / Q000528:radiation effects; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D015536:Down-Regulation; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008872:Microwaves; D062793:Occludin; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000378:metabolism* / Q000528:radiation effects*
chemical_list: D015815:Cell Adhesion Molecules; C520495:F11r protein, rat; D008565:Membrane Proteins; D062793:Occludin; C568987:Ocln protein, rat