Modulation of RNA metal binding by flanking bases: 15N NMR evaluation of GC, tandem GU, and tandem GA sites.
(15)N NMR chemical shift changes in the presence of Mg(H(2)O)(6)(2+), Zn(2+), Cd(2+), and Co(NH(3))(6)(3+) were used to probe the effect of flanking bases on metal binding sites in three different RNA motifs. We found that: for GC pairs, the presence of a flanking purine creates a site for the soft metals Zn(2+) and Cd(2+) only; a GG.UU motif selectively binds only Co(NH(3))(6)(3+), while a UG.GU motif binds none of these metals; a 3' guanosine flanking the adenosine of a sheared GA.AG pair creates an unusually strong binding site that precludes binding to the cross-strand stacked guanosines within the tandem pair.
Wang
Weimin
W
Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
Zhao
Jianwei
J
Han
Qianwei
Q
Wang
Gang
G
Yang
Gengcheng
G
Shallop
Anthony J
AJ
Liu
Jane
J
Gaffney
Barbara L
BL
Jones
Roger A
RA
D020029:Base Pairing; D001665:Binding Sites; D008670:Metals / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*
D008670:Metals; D012313:RNA