title: LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication.
abstract: LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3'-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions.
authors.affiliation: Department of Experimental and Health Sciences, Universitat Pompeu Fabra, 08003 Barcelona, Spain.
authors.forename: Rui Pedro
authors.initials: RP
authors.lastname: Galão
authors.forename: Ashwin
authors.initials: A
authors.lastname: Chari
authors.forename: Isabel
authors.initials: I
authors.lastname: Alves-Rodrigues
authors.forename: Daniela
authors.initials: D
authors.lastname: Lobão
authors.forename: Antonio
authors.initials: A
authors.lastname: Mas
authors.forename: Christian
authors.initials: C
authors.lastname: Kambach
authors.forename: Utz
authors.initials: U
authors.lastname: Fischer
authors.forename: Juana
authors.initials: J
authors.lastname: Díez
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D017795:Bromovirus / Q000235:genetics / Q000378:metabolism; D016679:Genome, Viral; D014176:Protein Biosynthesis; D039381:RNA Cap-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D019065:Virus Assembly; D014779:Virus Replication / Q000502:physiology*
chemical_list: D020413:3' Untranslated Regions; C114069:LSM1 protein, S cerevisiae; C521832:LSM7 protein, S cerevisiae; D039381:RNA Cap-Binding Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins