title: New design platform for malonyl-CoA-acyl carrier protein transacylase.
abstract: Malonyl-CoA-acyl carrier protein transacylase (MCAT) transfers the malonyl group from malonyl-CoA to holo-acyl carrier protein (ACP), and since malonyl-ACP is a key building block for fatty-acid biosynthesis it is considered as a promising antibacterial target. The crystal structures of MCAT from Staphylococcus aureus and Streptococcus pneumoniae have been determined at 1.46 and 2.1A resolution, respectively. In the SaMCAT structure, the N-terminal expression peptide of a neighboring molecule running in the opposite direction of malonyl-CoA makes extensive interactions with the highly conserved "Gly-Gln-Gly-Ser-Gln" stretch, suggesting a new design platform. Mutagenesis results suggest that Ser91 and His199 are the catalytic dyad.
authors.affiliation: Korea Institute of Science and Technology, Life Sciences Division, Seoul, Republic of Korea.
authors.forename: Seung Kon
authors.initials: SK
authors.lastname: Hong
authors.forename: Kook Han
authors.initials: KH
authors.lastname: Kim
authors.forename: Joon Kyu
authors.initials: JK
authors.lastname: Park
authors.forename: Ki-Woong
authors.initials: KW
authors.lastname: Jeong
authors.forename: Yangmee
authors.initials: Y
authors.lastname: Kim
authors.forename: Eunice EunKyeong
authors.initials: EE
authors.lastname: Kim
mesh_terms: D051080:Acyl-Carrier Protein S-Malonyltransferase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D020134:Catalytic Domain / Q000235:genetics; D018360:Crystallography, X-Ray; D015195:Drug Design; D057075:Enzyme Assays; D004791:Enzyme Inhibitors / Q000138:chemical synthesis*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009169:Mycobacterium tuberculosis / Q000201:enzymology / Q000235:genetics; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000235:genetics
chemical_list: D004791:Enzyme Inhibitors; D051080:Acyl-Carrier Protein S-Malonyltransferase