title: Polymerization of lignosulfonates by the laccase-HBT (1-hydroxybenzotriazole) system improves dispersibility.
abstract: The ability of laccases from Trametes villosa (TvL), Myceliophthora thermophila (MtL), Trametes hirsuta (ThL) and Bacillus subtilis (BsL) to improve the dispersion properties of calcium lignosulfonates 398 in the presence of HBT as a mediator was investigated. Size exclusion chromatography showed an extensive increase in molecular weight of the samples incubated with TvL and ThL by 107% and 572% from 28400 Da after 17h of incubation, respectively. Interestingly, FTIR spectroscopy, (13)C NMR and Py-GC/MS analysis of the treated samples suggested no substantial changes in the aromatic signal of the lignosulfonates, a good indication of the ability of TvL/ThL-HBT systems to limit their effect on functional groups without degrading the lignin backbone. Further, the enzymatic treatments led to a general increase in the dispersion properties, indeed a welcome development for its application in polymer blends.
authors.affiliation: Graz University of Technology, Institute of Environmental Biotechnology, Petersgasse 12/1, A-8010 Graz, Austria.
authors.forename: Endry
authors.initials: E
authors.lastname: Nugroho Prasetyo
authors.forename: Tukayi
authors.initials: T
authors.lastname: Kudanga
authors.forename: Lars
authors.initials: L
authors.lastname: Østergaard
authors.forename: Jorge
authors.initials: J
authors.lastname: Rencoret
authors.forename: Ana
authors.initials: A
authors.lastname: Gutiérrez
authors.forename: José C
authors.initials: JC
authors.lastname: del Río
authors.forename: J
authors.initials: J
authors.lastname: Ignacio Santos
authors.forename: Lidia
authors.initials: L
authors.lastname: Nieto
authors.forename: Jesús
authors.initials: J
authors.lastname: Jiménez-Barbero
authors.forename: Angel T
authors.initials: AT
authors.lastname: Martínez
authors.forename: Jiebing
authors.initials: J
authors.lastname: Li
authors.forename: Göran
authors.initials: G
authors.lastname: Gellerstedt
authors.forename: Stéphane
authors.initials: S
authors.lastname: Lepifre
authors.forename: Carla
authors.initials: C
authors.lastname: Silva
authors.forename: Su Yeon
authors.initials: SY
authors.lastname: Kim
authors.forename: Artur
authors.initials: A
authors.lastname: Cavaco-Paulo
authors.forename: Bente
authors.initials: B
authors.lastname: Seljebakken Klausen
authors.forename: Bjart Frode
authors.initials: BF
authors.lastname: Lutnaes
authors.forename: Gibson S
authors.initials: GS
authors.lastname: Nyanhongo
authors.forename: Georg M
authors.initials: GM
authors.lastname: Guebitz
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism; D001673:Biodegradation, Environmental; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D042845:Laccase / Q000737:chemistry*; D008031:Lignin / Q000031:analogs & derivatives* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D017785:Photons; D011108:Polymers / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013450:Sulfones / Q000737:chemistry; D013997:Time Factors; D014230:Triazoles / Q000737:chemistry*; D018508:Water Purification / Q000379:methods*
chemical_list: D011108:Polymers; D013450:Sulfones; D014230:Triazoles; C001545:lignosulfuric acid; D008031:Lignin; C011852:1-hydroxybenzotriazole; D042845:Laccase