title: Interaction of a host protein with core complexes of bacteriophage phi6 to control transcription.
abstract: Bacteriophages of the family Cystoviridae have genomes consisting of three double-stranded RNA (dsRNA) segments, L, S, and M, packaged within a polyhedral capsid along with RNA polymerase. Transcription of genomic segment L is activated by the interaction of host protein YajQ with the capsid structure. Segment L codes for the proteins of the inner capsid, which are expressed early in infection. Green fluorescent protein (GFP) fusions with YajQ produce uniform fluorescence in uninfected cells and in cells infected with viruses not dependent on YajQ. Punctate fluorescence develops when cells are infected with YajQ-dependent viruses. It appears that the host protein binds to the infecting particles and remains with them during the entire infection period.
authors.affiliation: Public Health Research Institute Center at UMDNJ, 225 Warren Street, Newark, NJ 07103, USA.
authors.forename: Xueying
authors.initials: X
authors.lastname: Qiao
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.forename: Jian
authors.initials: J
authors.lastname: Qiao
authors.forename: Leonard
authors.initials: L
authors.lastname: Mindich
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D017126:Bacteriophage phi 6 / Q000502:physiology*; D036022:Capsid Proteins / Q000378:metabolism*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D044224:Pseudomonas syringae / Q000821:virology*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D036022:Capsid Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins