Attenuation of experimental murine colitis by acanthoic acid from Acanthopanax koreanum.
Acanthoic acid (AA) is a pimaradiene diterpene isolated from Acanthopanax koreanum. We examined the effect of AA in dextran sulfate sodium (DSS)-induced colitis. AA (100 mg/kg or 300 mg/kg) was administered p.o. daily for 7 days. AA significantly inhibited Disease Activity Index, histological score, and myeloperoxidase activity. Furthermore, AA markedly suppressed the protein expression of TNF-alpha, COX-2, NF-kappaB and chymase as well as the mRNA expression of TNF-alpha and COX-2. These results suggest that AA exerts beneficial effects in experimental colitis, and therefore we propose that this compound may have therapeutic implications for ulcerative colitis.
Kang
Ok-Hwa
OH
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang-Oriental Medicines Research Institute, Iksan 570-749, Korea.
Kim
Dae-Ki
DK
Cai
Xing-Fu
XF
Kim
Young-Ho
YH
Lee
Young-Mi
YM
D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D053818:Chymases / Q000096:biosynthesis; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000473:pathology; D003106:Colon / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D003967:Diarrhea / Q000188:drug therapy / Q000209:etiology; D004224:Diterpenes / Q000737:chemistry* / Q000627:therapeutic use*; D028001:Eleutherococcus / Q000737:chemistry*; D005260:Female; D006470:Hemorrhage / Q000188:drug therapy / Q000209:etiology; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000096:biosynthesis; D009195:Peroxidase / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D004224:Diterpenes; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C099958:acanthoic acid; D009195:Peroxidase; D051546:Cyclooxygenase 2; D053818:Chymases