Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso.
Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV) isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned and sequenced. Six betasatellite and four alphasatellite (DNA-1) molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite (CLCuGB) (88 to 98% nucleotide identity). One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite (CLCuGA) (89% nucleotide identity), whereas the three others isolates appear to correspond to a new species of alphasatellite (CLCuGA most similar sequence present 52 to 60% nucleotide identity), provisionally named Okra leaf curl Burkina Faso alphasatellite (OLCBFA). Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates, with parents being close to Hollyhock leaf crumple virus (AY036009) and Tomato leaf curl Diana virus (AM701765). Combined with the presence of satellites DNA, these results highlight the complexity of begomoviruses associated with OLCD.
Tiendrébéogo
Fidèle
F
Laboratoire de Biochimie & Biologie Moléculaire, CRSBAN/UFR/SVT, Université de Ouagadougou 03 BP 7021 Ouagadougou 03, Burkina Faso. fidelet@gmail.com
Lefeuvre
Pierre
P
Hoareau
Murielle
M
Villemot
Julie
J
Konaté
Gnissa
G
Traoré
Alfred S
AS
Barro
Nicolas
N
Traoré
Valentin S
VS
Reynaud
Bernard
B
Traoré
Oumar
O
Lett
Jean-Michel
JM
D028483:Abelmoschus / Q000821:virology*; D053839:Begomovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002050:Burkina Faso; D016000:Cluster Analysis; D004276:DNA, Satellite / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004276:DNA, Satellite; D004279:DNA, Viral