Evaluation [corrected] of transgenic event CBH 351 (StarLink) corn in pig.
This study examined the influence of transgenic event CBH (StarLink; SL)-derived hybrid corn on growth, health and physiological functions of pigs, as well as the possibility of transferring the cry9C gene or Cry9C protein to the blood, liver or muscles, in comparison with pigs fed a diet with non-transgenic (isogenic) corn (non-SL). The diet for the SL group was composed of 70% SL corn, and the diet for the non-SL group was composed of 70% non-SL corn. Forty pigs approximately 3 months in age were used in the current experiment. After the pigs were acclimatized to their environment for 7 days, they were fed piglet diets for 7 weeks, and afterwards fed growing-finishing diets until the end of the experiment. There were no significant differences in bodyweight gain, feed intake or feed conversion ratio between the pigs fed SL diet and those of non-SL diet. No abnormalities were observed in the health conditions of either the SL or the non-SL group. Moreover, no significant differences were observed between the two groups in hematological values, histopathological examination and necropsy findings. Although the serum biochemical values within each group were normal, the blood urea nitrogen values of the SL group showed a tendency to be slightly higher than those of the non-SL group. Also, the blood glucose values of the SL group were significantly lower than those of the non-SL group. However, the cause of the significant differences in the blood glucose values between the two groups is unknown. The PCR and ELISA did not detect the cry9C gene and Cry9C protein in the blood, liver or muscles of the pigs at the end of the experiment.
Yonemochi
Chisato
C
Japan Scientific Feeds Association, Chuo, Tokyo, Japan. yone@kashikyo.or.jp
Suga
Keisei
K
Harada
Chisato
C
Hanazumi
Michito
M
D000821:Animal Feed; D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001786:Blood Glucose; D001806:Blood Urea Nitrogen; D004269:DNA, Bacterial; D004435:Eating / Q000523:psychology; D004731:Endotoxins / Q000235:genetics*; D006403:Hematologic Tests; D006460:Hemolysin Proteins / Q000235:genetics*; D009753:Nutritive Value; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D034421:Sus scrofa; D015430:Weight Gain / Q000502:physiology*; D003313:Zea mays / Q000235:genetics / Q000378:metabolism
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001786:Blood Glucose; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis