Do mitochondria in Dendrobium petal mesophyll cells form vacuole-like vesicles?
Using transmission electron microscopy, we investigated the ultrastructure of mitochondria in petal mesophyll cells of the orchid Dendrobium cv. Lucky Duan, from the time of floral opening to visible petal senescence. Cells close to the vascular bundle contained many mitochondria, some of which showed internal degeneration. This inner mitochondrial breakdown was accompanied by an eightfold increase in mitochondrial volume. Small electron-dense granules (approximately 0.04 mum in diameter) at the periphery of the mitochondrial matrix remained. These granules were used as an indicator of still later stages of mitochondrial development in these cells. The apparent final stage of mitochondrial degeneration was a single-membrane-bound vesicle, resembling a vacuole. No evidence was found for the idea that mitochondria became transferred (intact or degenerated) into a lytic vacuole. Taken together, the data suggest the hypotheses that (a) mitochondria in cells close to the vascular bundle in petals of open Dendrobium cv. Lucky Duan flowers undergo large-scale internal degeneration and that (b) such degenerating mitochondria form vacuole-like vesicles.
Kirasak
Kanjana
K
Department of Horticulture, Kasetsart University, Kamphaeng Saen campus, Nakhon Pathom 73140, Thailand.
Ketsa
Saichol
S
Imsabai
Wachiraya
W
van Doorn
Wouter G
WG
D031667:Dendrobium / Q000648:ultrastructure*; D035264:Flowers / Q000648:ultrastructure*; D008853:Microscopy; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000648:ultrastructure*