title: CKAMP44: a brain-specific protein attenuating short-term synaptic plasticity in the dentate gyrus.
abstract: CKAMP44, identified here by a proteomic approach, is a brain-specific type I transmembrane protein that associates with AMPA receptors in synaptic spines. CKAMP44 expressed in Xenopus oocytes reduced GluA1- and A2-mediated steady-state currents, but did not affect kainate- or N-methyl-D-aspartate (NMDA) receptor-mediated currents. Mouse hippocampal CA1 pyramidal neurons expressed CKAMP44 at low abundance, and overexpression of CKAMP44 led to stronger and faster AMPA receptor desensitization, slower recovery from desensitization, and a reduction in the paired-pulse ratio of AMPA currents. By contrast, dentate gyrus granule cells exhibited strong CKAMP44 expression, and CKAMP44 knockout increased the paired-pulse ratio of AMPA currents in lateral and medial perforant path-granule cell synapses. CKAMP44 thus modulates short-term plasticity at specific excitatory synapses.
authors.affiliation: Department of Clinical Neurobiology, University of Heidelberg, 6910 Heidelberg, Germany.
authors.forename: Jakob
authors.initials: J
authors.lastname: von Engelhardt
authors.forename: Volker
authors.initials: V
authors.lastname: Mack
authors.forename: Rolf
authors.initials: R
authors.lastname: Sprengel
authors.forename: Netta
authors.initials: N
authors.lastname: Kavenstock
authors.forename: Ka Wan
authors.initials: KW
authors.lastname: Li
authors.forename: Yael
authors.initials: Y
authors.lastname: Stern-Bach
authors.forename: August B
authors.initials: AB
authors.lastname: Smit
authors.forename: Peter H
authors.initials: PH
authors.lastname: Seeburg
authors.forename: Hannah
authors.initials: H
authors.lastname: Monyer
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D049229:Dendritic Spines / Q000378:metabolism; D018891:Dentate Gyrus / Q000166:cytology / Q000378:metabolism*; D000076122:Disks Large Homolog 4 Protein; D019706:Excitatory Postsynaptic Potentials; D018698:Glutamic Acid / Q000378:metabolism; D051528:Guanylate Kinases; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D055369:Miniature Postsynaptic Potentials; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009433:Neural Inhibition; D009473:Neuronal Plasticity; D009474:Neurons / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D018408:Patch-Clamp Techniques; D019580:Perforant Pathway; D054730:Protein Interaction Domains and Motifs; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D017966:Pyramidal Cells / Q000378:metabolism; D018091:Receptors, AMPA / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission; D014982:Xenopus laevis
chemical_list: C548320:CKAMP44 protein, mouse; C113940:Cacng2 protein, mouse; D015220:Calcium Channels; D000076122:Disks Large Homolog 4 Protein; C501447:Dlg4 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D018091:Receptors, AMPA; D011993:Recombinant Fusion Proteins; D018698:Glutamic Acid; D051528:Guanylate Kinases