title: Effects of surface chemistry prepared by self-assembled monolayers on osteoblast behavior.
abstract: A surface of biomaterials is known to affect the behavior of cells after their adhesion on the surface, indicating that surface characteristics of biomaterials play an important role in cell adhesion, proliferation, and differentiation. To assess the effects of functional groups on biomaterial surface, normal human osteoblasts (NHOsts) were cultured on surfaces coated with self-assembled monolayers (SAMs) containing various functional groups, and the adhesion, proliferation, differentiation, and gap junctional intercellular communication (GJIC) of the NHOsts were investigated. In the case of SAM with terminal methyl groups (hydrophobic surface), NHOst adhesion and proliferation was less prevalent. In contrast, NHOsts were adhered well on SAMs with hydroxyl, carboxyl, amino, phosphate, and sulfate group, which are relatively hydrophilic, their proliferation and differentiation level were dependent on the type of functional groups. Especially, when they were cultured on either SAMs with phosphate or sulfate group, both their alkaline phosphate activity and the calcium deposition by them were enhanced more than those cultured on a collagen-coated dish. More interestingly, GJIC of NHOsts, which has been reported to play a role in cell differentiation as well as homeostasis of cells, were not significantly different among the SAM surfaces tested. These suggest that a specific functional group on a material surface can regulate NHOst adhesion, proliferation, and differentiation via cell-functional group interaction without influencing their homeostasis.
authors.affiliation: Division of Medical Devices, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. nakaoka@nihs.go.jp
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Nakaoka
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yamakoshi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Isama
authors.forename: Toshie
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002448:Cell Adhesion; D002450:Cell Communication; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D018031:Connexin 43 / Q000235:genetics / Q000378:metabolism; D017629:Gap Junctions / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D015394:Molecular Structure; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D018031:Connexin 43; C500216:GJA1 protein, human