Photonic methods to enhance fluorescence correlation spectroscopy and single molecule fluorescence detection.
Recent advances in nanophotonics open the way for promising applications towards efficient single molecule fluorescence analysis. In this review, we discuss how photonic methods bring innovative solutions for two essential questions: how to detect a single molecule in a highly concentrated solution, and how to enhance the faint optical signal emitted per molecule? The focus is set primarily on the widely used technique of fluorescence correlation spectroscopy (FCS), yet the discussion can be extended to other single molecule detection methods.
Wenger
Jérome
J
Institut Fresnel, Aix-Marseille Université, CNRS, Ecole Centrale Marseille, Domaine Universitaire de Saint-Jérôme, 13397 Marseille cedex 20, France.
Rigneault
Hervé
H
Institut Fresnel, Aix-Marseille Université, CNRS, Ecole Centrale Marseille, Domaine Universitaire de Saint-Jérôme, 13397 Marseille cedex 20, France.
D055095:Optics and Photonics / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*