title: Voltammetric detection of damage to DNA caused by nitro derivatives of fluorene using an electrochemical DNA biosensor.
abstract: An electrochemical DNA biosensor based on the screen printed carbon paste electrode (SPCPE) with an immobilized layer of calf thymus double-stranded DNA has been used for in vitro investigation of the interaction between genotoxic nitro derivatives of fluorene (namely 2-nitrofluorene and 2,7-dinitrofluorene) and DNA. Two types of DNA damage have been detected at the DNA/SPCPE biosensor: first, that caused by direct association of the nitrofluorenes, for which an intercalation association has been found using the known DNA intercalators [Cu(phen)(2)](2+) and [Co(phen)(3)](3+) as competing agents, and, second, that caused by short-lived radicals generated by electrochemical reduction of the nitro group (observable under specific conditions only).
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Hlavova 2030/8, 12843, Prague 2, Czech Republic. vyskoci1@natur.cuni.cz.
authors.forename: Vlastimil
authors.initials: V
authors.lastname: Vyskočil
authors.affiliation: Slovak University of Technology in Bratislava, Faculty of Chemical and Food Technology, Institute of Analytical Chemistry, Radlinského 9, 81237, Bratislava, Slovakia.
authors.forename: Ján
authors.initials: J
authors.lastname: Labuda
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Hlavova 2030/8, 12843, Prague 2, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Barek
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004249:DNA Damage / Q000235:genetics*; D004563:Electrochemistry; D005449:Fluorenes / Q000494:pharmacology*; D007202:Indicators and Reagents / Q000032:analysis / Q000737:chemistry
chemical_list: D005449:Fluorenes; D007202:Indicators and Reagents; C085049:2,7-dinitrofluorene; D004247:DNA; C427203:calf thymus DNA