Two-photon uncaging of gamma-aminobutyric acid in intact brain tissue.
We have synthesized a photosensitive (or caged) 4-carboxymethoxy-5,7-dinitroindolinyl (CDNI) derivative of gamma-aminobutyric acid (GABA). Two-photon excitation of CDNI-GABA produced rapid activation of GABAergic currents in neurons in brain slices with an axial resolution of approximately 2 mum and enabled high-resolution functional mapping of GABA-A receptors. Two-photon uncaging of GABA, the main inhibitory neurotransmitter, should allow detailed studies of receptor function and synaptic integration with subcellular precision.
Matsuzaki
Masanori
M
Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 003-1133, Japan, and Center for NanoBio Integration, University of Tokyo, Tokyo, Japan.
Hayama
Tatsuya
T
Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 003-1133, Japan, and Center for NanoBio Integration, University of Tokyo, Tokyo, Japan.
Kasai
Haruo
H
Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 003-1133, Japan, and Center for NanoBio Integration, University of Tokyo, Tokyo, Japan.
Ellis-Davies
Graham C R
GCR
Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, PA 19102, USA.
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D009474:Neurons / Q000378:metabolism / Q000528:radiation effects; D010777:Photochemistry; D010782:Photolysis / Q000528:radiation effects; D017785:Photons; D051381:Rats; D011963:Receptors, GABA-A / Q000737:chemistry / Q000378:metabolism; D014466:Ultraviolet Rays; D005680:gamma-Aminobutyric Acid / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*
D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid