title: Mobilization and collection of peripheral blood stem cells: guidelines for blood volume to process, based on CD34-positive blood cell count in adults and children.
abstract: We report 189 mobilizations and 489 collections of peripheral blood stem cells (PBSC) performed in 139 autologous transplantation patients and in 28 donors for allogeneic transplantations whose ages ranged from 2-68 years. We observed a correlation (P < .001; Pearson's coefficient 0.64) between CD34-positive cells and granulocyte-macrophage colony-forming units examined to estimate PBSC. In a subset of 287 collections (97 adults and 49 children) we obtained peripheral blood (PB) CD34-positive cell counts at 2 to 4 hours before leukapheresis. We noted a correlation between PB CD34-positive cell counts before leukapheresis and the number of CD34-positive cells per kilogram of body weight collected in the whole apheresis of the day (P < .001; Pearson's coefficient 0.82). An even better correlation was obtained between PB CD34-positive cells preapheresis and the yield of each individual blood volume (BV) processed (P < .001; Pearson's coefficient 0.87). Healthy donors and patients in each age group behaved similarly. In addition, the collection yield was greater among children than adults. These findings allowed us to develop a simple predictive model to estimate the BV to process for a target dose of CD34-positive cells per kilogram, based on the level of PBSC before apheresis in children and adults.
authors.affiliation: Unidad de Trasplante de Progenitores Hematopoyeticos, Clinica Alemana de Santiago, Santiago, Chile. atanguit@gmail.com
authors.forename: A T
authors.initials: AT
authors.lastname: Anguita-Compagnon
authors.forename: M T
authors.initials: MT
authors.lastname: Dibarrart
authors.forename: J
authors.initials: J
authors.lastname: Palma
authors.forename: L
authors.initials: L
authors.lastname: Paredes
authors.forename: C
authors.initials: C
authors.lastname: Mosso
authors.forename: R
authors.initials: R
authors.lastname: Montalva
authors.forename: L
authors.initials: L
authors.lastname: Salas
authors.forename: D
authors.initials: D
authors.lastname: Araos
authors.forename: I
authors.initials: I
authors.lastname: Delgado
authors.forename: A
authors.initials: A
authors.lastname: Majlis
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000097:blood*; D001810:Blood Volume; D002452:Cell Count; D002648:Child; D002675:Child, Preschool; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D007937:Leukapheresis / Q000379:methods; D008875:Middle Aged; D017410:Practice Guidelines as Topic; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D014019:Tissue Donors; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
chemical_list: D018952:Antigens, CD34