Impact of cladribine on soluble adhesion molecules in multiple sclerosis.
BACKGROUND
Soluble forms of vascular cell adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1) and E-Selectin play a role in the regulation of blood-brain barrier damage and represent markers of the clinical course of multiple sclerosis (MS) and magnetic resonance imaging activity. We determined sICAM, sVCAM and sE-Selectin concentrations in the cerebrospinal fluid (CSF) and serum of patients with remitting-relapsing multiple sclerosis before and after cladribine treatment as well as in a control group.


METHODS
We examined 17 patients diagnosed according to McDonald's criteria. Thirteen healthy age-matched subjects served as controls. The ELISA method was used to measure sICAM-1, sVCAM-1 and sE-Selectin.


RESULTS
The concentration of sICAM and sE-Selectin decreased in sera (difference between patients and controls was statistically significant, in the former P < 0.04, in the latter P < 0.0003) but not in the CSF of MS patients after cladribine treatment.


CONCLUSIONS
The reduction in sICAM and sE-Selectin concentrations after cladribine treatment indicates an immuno-suppressive effect of the drug. The changes in levels of sICAM and sE-Selectin after cladribine treatment reflect disease activity and indicate a reduction in the inflammatory reaction.
Mitosek-Szewczyk
Krystyna
K
Department of Neurology, Medical University in Lublin, Lublin, Poland. krystyna.mitosek@am.lublin.pl
Stelmasiak
Z
Z
Bartosik-Psujek
H
H
Belniak
E
E
D000328:Adult; D016022:Case-Control Studies; D017338:Cladribine / Q000627:therapeutic use; D004185:Disability Evaluation; D019040:E-Selectin / Q000134:cerebrospinal fluid*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000188:drug therapy; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood*; D055815:Young Adult
D019040:E-Selectin; D007166:Immunosuppressive Agents; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D017338:Cladribine