Increased phosphorylation of vimentin in noninfiltrative meningiomas.
BACKGROUND
Tissue invasion or tissue infiltration are clinical behaviors of a poor-prognosis subset of meningiomas. We carried out proteomic analyses of tissue extracts to discover new markers to accurately distinguish between infiltrative and noninfiltrative meningiomas.


RESULTS
Protein lysates of 64 different tissue samples (including two brain-invasive and 32 infiltrative tumors) were submitted to SELDI-TOF mass spectrometric analysis. Mass profiles were used to build up both unsupervised and supervised hierarchical clustering. One marker was found at high levels in noninvasive and noninfiltrative tumors and appeared to be a discriminative marker for clustering infiltrative and/or invasive meningiomas versus noninvasive meningiomas in two distinct subsets. Sensitivity and specificity were 86.7% and 100%, respectively. This marker was purified and identified as a multiphosphorylated form of vimentin, a cytoskeletal protein expressed in meningiomas.


CONCLUSIONS
Specific forms of vimentin can be surrogate molecular indicators of the invasive/infiltrative phenotype in tumors.
Bouamrani
Ali
A
Grenoble Institut des Neurosciences, INSERM U836, Université Joseph Fourier, Grenoble, France.
Ramus
Claire
C
Gay
Emmanuel
E
Pelletier
Laurent
L
Cubizolles
Myriam
M
Brugière
Sabine
S
Wion
Didier
D
Berger
François
F
Issartel
Jean-Paul
JP
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000378:metabolism*; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000145:classification / Q000175:diagnosis / Q000378:metabolism*; D008579:Meningioma / Q000145:classification / Q000175:diagnosis / Q000378:metabolism*; D008875:Middle Aged; D008970:Molecular Weight; D010766:Phosphorylation; D040901:Proteomics / Q000379:methods; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014746:Vimentin / Q000737:chemistry / Q000378:metabolism*
D014408:Biomarkers, Tumor; D014746:Vimentin