Induction of apoptosis by [8]-shogaol via reactive oxygen species generation, glutathione depletion, and caspase activation in human leukemia cells.
Ginger, the rhizome of Zingiber officinale , is a traditional medicine with a carminative effect and antinausea, anti-inflammatory, and anticarcinogenic properties. This study examined the growth inhibitory effects of [8]-shogaol, one of the pungent phenolic compounds in ginger, on human leukemia HL-60 cells. It demonstrated that [8]-shogaol was able to induce apoptosis in a time- and concentration-dependent manner. Treatment with [8]-shogaol caused a rapid loss of mitochondrial transmembrane potential, stimulation of reactive oxygen species (ROS) production, release of mitochondrial cytochrome c into cytosol, and subsequent induction of procaspase-9 and procaspase-3 processing. Taken together, these results suggest for the first time that ROS production and depletion of glutathione that contributed to [8]-shogaol-induced apoptosis in HL-60 cells.
Shieh
Po-Chuen
PC
Department of Pharmacy and Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung, Taiwan. shea7166@hotmail.com
Chen
Yi-Own
YO
Kuo
Daih-Huang
DH
Chen
Fu-An
FA
Tsai
Mei-Ling
ML
Chang
Ing-Shing
IS
Wu
Hou
H
Sang
Shengmin
S
Ho
Chi-Tang
CT
Pan
Min-Hsiung
MH
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism*; D053453:Caspase 9 / Q000378:metabolism*; D002396:Catechols / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004789:Enzyme Activation / Q000187:drug effects; D020939:Zingiber officinale / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000201:enzymology / Q000378:metabolism / Q000503:physiopathology*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
D002396:Catechols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C040115:shogaol; D053148:Caspase 3; D053453:Caspase 9; D005978:Glutathione