The glycoproteins C and G are equivalent target antigens for the determination of herpes simplex virus type 1-specific antibodies.
Seroreactivity to the glycoproteins C and G of herpes simplex virus type 1 (HSV-1) was compared in 310 serum samples using a Western blot assay containing a whole antigen extract of HSV-1 and an ELISA employing gC1 isolated from HSV-1. The prevalence of reactivity to gC1 was 75.8% by Western blot and 73.9% by ELISA, while antibody responses to gG1 were detected in 72.9% of sera by Western blot. An absolute correlation of 96.1% between the reactivity to gC1 and gG1 was demonstrated using the Western blot. The gC1-based ELISA correlated with Western blot detection of anti-gC1 and anti-gG1 antibodies in 95.2 and 97.7% of samples, respectively. 3.2% of all sera were reactive with gC1 in Western blot and/or ELISA, but were negative for anti-gG1. For analysis of cross-reactivity, antibodies against HSV-2, Epstein-Barr virus, varicella-zoster virus and cytomegalovirus were determined. The prevalence of antibodies against each individual virus was identical in the groups of sera reactive with gC1 or gG1. These findings indicate that gC1 and gG1 are equivalent antigenic targets for the type-specific serodiagnosis of HSV-1 infections.
Scheper
Thomas
T
Institute of Experimental Immunology, affiliated to EUROIMMUN AG, Seekamp 31, D-23560 Luebeck, Germany.
Saschenbrecker
Sandra
S
Steinhagen
Katja
K
Sauerbrei
Andreas
A
Suer
Waltraud
W
Meyer
Wolfgang
W
Schlumberger
Wolfgang
W
Wandinger
Klaus-Peter
KP
D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D015153:Blotting, Western / Q000379:methods; D003587:Cytomegalovirus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006561:Herpes Simplex / Q000175:diagnosis* / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000276:immunology; D014645:Herpesvirus 3, Human / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; C050591:glycoprotein gG-1, herpes simplex virus type 1