title: High GC contents of primer 5'-end increases reaction efficiency in polymerase chain reaction.
abstract: Many studies have suggested that regulation of the polymerase chain reaction (PCR) is influenced by several factors. However, the understanding of reaction efficiency factors is not sufficient. Here we propose that high GC contents of primer 5'-end increases reaction efficiency in PCR. Using 71 primers (45 pairs), we analyzed factors that affect reaction efficiency, and statistically tested the correlation between the amplification signals and several factors. As a result, there were significant correlations between the amplification signals and the GC contents in the first 1 approximately 3 bps of primer 5'-end.
authors.affiliation: Food and Agricultural Materials Inspection Center, Saitama, Japan. naoki_shinoda@nm.famic.go.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Shinoda
authors.forename: Tomotaro
authors.initials: T
authors.lastname: Yoshida
authors.forename: Toyoko
authors.initials: T
authors.lastname: Kusama
authors.forename: Masami
authors.initials: M
authors.lastname: Takagi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Onodera
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Sugiura
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000737:chemistry*; D004272:DNA, Mitochondrial / Q000737:chemistry; D020862:GC Rich Sequence; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial