title: Epitope-cavities generated by molecularly imprinted films measure the coincident response to anthrax protective antigen and its segments.
abstract: A molecularly imprinted film was fabricated, in the presence of epitope-peptides, onto a quartz crystal microbalance (QCM) chip. These five peptides are known linear or conformational epitopes of the anthrax protective antigen PA(83). Imprinting resulted in an epitope-cavity with affinity for the corresponding template. With the use of a basic monomer, the binding-effect was further enhanced increasing the affinity to nanomolar levels. The affinities of the peptide to their corresponding molecularly induced polymers (MIPs) were more closely related to the molecular weight of the analyte than to the number of residues. All epitope-cavities differentiated their epitope region on the protective antigen PA(83) as well as the corresponding furin cleavage fragments PA(63) and PA(20). The QCM chip differential response to the protective antigen fragment was observed in the picomolar range, thus demonstrating a method to manipulate protein on the surface with defined orientation.
authors.affiliation: Department of Chemistry, National Dong-Hwa University, Hualien, Taiwan. dftai@mail.ndhu.edu.tw
authors.forename: Dar-Fu
authors.initials: DF
authors.lastname: Tai
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Jhang
authors.forename: Guan-Yu
authors.initials: GY
authors.lastname: Chen
authors.forename: Sue-Chen
authors.initials: SC
authors.lastname: Wang
authors.forename: Kuo-Hao
authors.initials: KH
authors.lastname: Lu
authors.forename: Yu-Der
authors.initials: YD
authors.lastname: Lee
authors.forename: Hsin-Tzu
authors.initials: HT
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001427:Bacterial Toxins / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D054802:Molecular Imprinting; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D011791:Quartz / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D000939:Epitopes; D010455:Peptides; C030325:anthrax toxin; D011791:Quartz