Hydrazone ligation strategy to assemble multifunctional viral nanoparticles for cell imaging and tumor targeting.
Multivalent nanoparticle platforms are attractive for biomedical applications because of their improved target specificity, sensitivity, and solubility. However, their controlled assembly remains a considerable challenge. An efficient hydrazone ligation chemistry was applied to the assembly of Cowpea mosaic virus (CPMV) nanoparticles with individually tunable levels of a VEGFR-1 ligand and a fluorescent PEGylated peptide. The nanoparticles recognized VEGFR-1 on endothelial cell lines and VEGFR1-expressing tumor xenografts in mice, validating targeted CPMV as a nanoparticle platform in vivo.
Brunel
Florence M
FM
Department of Cell Biology.
Lewis
John D
JD
Destito
Giuseppe
G
Steinmetz
Nicole F
NF
Manchester
Marianne
M
Stuhlmann
Heidi
H
Dawson
Philip E
PE
D000818:Animals; D016503:Drug Delivery Systems / Q000379:methods*; D019073:HT29 Cells; D006801:Humans; D006835:Hydrazones / Q000737:chemistry*; D007089:Image Enhancement / Q000379:methods*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D014771:Virion / Q000737:chemistry*
D006835:Hydrazones