title: Extending synthetic routes for oligosaccharides by enzyme, substrate and reaction engineering.
abstract: The integration of all relevant tools for bioreaction engineering has been a recent challenge. This approach should notably favor the production of oligo- and polysaccharides, which is highly complex due to the requirements of regio- and stereoselectivity. Oligosaccharides (OS) and polysaccharides (PS) have found many interests in the fields of food, pharmaceuticals, and cosmetics due to different specific properties. Food, sweeteners, and food ingredients represent important sectors where OS are used in major amounts. Increasing attention has been devoted to the sophisticated roles of OS and glycosylated compounds, at cell or membrane surfaces, and their function, e.g., in infection and cancer proliferation. The challenge for synthesis is obvious, and convenient approaches using cheap and readily available substrates and enzymes will be discussed. We report on new routes for the synthesis of oligosaccharides (OS), with emphasis on enzymatic reactions, since they offer unique properties, proceeding highly regio- and stereoselective in water solution, and providing for high yields in general.
authors.affiliation: Institute of Organic Chemistry, University of Würzburg, Am Hubland, 97074, Würzburg, Germany, seibel@chemie.uni-wuerzburg.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Seibel
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Jördening
authors.forename: Klaus
authors.initials: K
authors.lastname: Buchholz
mesh_terms: D032701:Biomimetics / Q000379:methods*; D004789:Enzyme Activation; D016695:Glycosyltransferases / Q000737:chemistry*; D006602:Hexosyltransferases / Q000737:chemistry*; D009844:Oligosaccharides / Q000138:chemical synthesis*; D011134:Polysaccharides / Q000138:chemical synthesis*; D013379:Substrate Specificity; D058615:Synthetic Biology / Q000379:methods*; D001426:Bacterial Proteins
chemical_list: D016695:Glycosyltransferases; D006602:Hexosyltransferases; D009844:Oligosaccharides; D011134:Polysaccharides; C021451:inulosucrase; D001426:Bacterial Proteins