title: Glycoprofiling of the Human Salivary Proteome.
abstract: Glycosylation is important for a number of biological processes and is perhaps the most abundant and complicated of the known post-translational modifications found on proteins. This work combines two-dimensional polyacrylamide gel electrophoresis (2-DE) and lectin blotting to map the salivary glycome, and mass spectrometry to identity the proteins that are associated with the glycome map. A panel of 15 lectins that recognize six sugar-specific categories was used to visualize the type and extent of glycosylation in saliva from two healthy male individuals. Lectin blots were compared to 2-D gels stained either with Sypro Ruby (protein stain) or Pro-Q Emerald 488 (glycoprotein stain). Each lectin shows a distinct pattern, even those belonging to the same sugar-specific category. In addition, the glycosylation profiles generated from the lectin blots show that most of the salivary proteins are glycosylated and that the pattern is more widespread than is demonstrated by the glycoprotein stained gel. Finally, the co-reactivity between two lectins was measured to determine the glycan structures that are most and least often associated with one another along with the population variation of the lectin reactivity for 66 individuals.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Los Angeles, CA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Sondej
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Denny
authors.forename: Yongming
authors.initials: Y
authors.lastname: Xie
authors.forename: Prasanna
authors.initials: P
authors.lastname: Ramachandran
authors.forename: Yan
authors.initials: Y
authors.lastname: Si
authors.forename: Jona
authors.initials: J
authors.lastname: Takashima
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Shi
authors.forename: David T
authors.initials: DT
authors.lastname: Wong
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Loo
authors.forename: Paul C
authors.initials: PC
authors.lastname: Denny