title: An improved protocol for the preparation of total genomic DNA from isolates of yeast and mould using Whatman FTA filter papers.
abstract: Here, we present a significantly improved version of our previously published method for the extraction of fungal genomic DNA from pure cultures using Whatman FTA filter paper matrix technology. This modified protocol is extremely rapid, significantly more cost effective than our original method, and importantly, substantially reduces the problem of potential cross-contamination between sequential filters when employing FTA technology.
authors.affiliation: Mycology Reference Laboratory, Health Protection Agency, South-West Regional Laboratory, Bristol, UK. Andy.Borman@uhBristol.nhs.uk
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Borman
authors.forename: Mark
authors.initials: M
authors.lastname: Fraser
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Linton
authors.forename: Michael D
authors.initials: MD
authors.lastname: Palmer
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Johnson
mesh_terms: D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification*; D005374:Filtration / Q000295:instrumentation*; D005658:Fungi / Q000235:genetics / Q000302:isolation & purification; D016681:Genome, Fungal; D006801:Humans; D009181:Mycoses / Q000175:diagnosis / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D013048:Specimen Handling; D013997:Time Factors; D015003:Yeasts / Q000235:genetics / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D004271:DNA, Fungal