title: Measurement of milk D-3-hydroxybutyrate with a simple UV spectrophotometer method: an alternative assay method.
abstract: The measurement of D-3-hydroxybutyrate (D-BHBA) in milk samples is an important tool for diagnosis of subclinical/clinical ketosis in dairy cows. We describe a simple UV spectrophotometric method for measuring the concentration of D-BHBA in milk of dairy cows. From two herds, 119 milk samples were taken from dairy cows. The standard-curve equation was y = 0.2582x + 0.0269 (R2 = 0.9967). The assay was highly specific with a minimum detection limit of 0.01 mmol/L and measuring range of up to 5 mmol/L. The recovery was between 99.35% and 100.22% and repeatability was 99.8%. The comparison between the spectrophotometric method and the fluorometric method revealed a close correlation (r = 0.9939). These results show that the spectrophotometric method can be successfully used as an alternative method to measure D-BHBA content in milk.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, 5333 of Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Zhang
authors.forename: G W
authors.initials: GW
authors.lastname: Liu
authors.forename: X B
authors.initials: XB
authors.lastname: Li
authors.forename: Y F
authors.initials: YF
authors.lastname: Li
authors.forename: C M
authors.initials: CM
authors.lastname: Guo
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.forename: H B
authors.initials: HB
authors.lastname: Wang
authors.forename: S W
authors.initials: SW
authors.lastname: Xu
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000032:analysis*; D000818:Animals; D002417:Cattle; D005260:Female; D008892:Milk / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods / Q000662:veterinary*
chemical_list: D020155:3-Hydroxybutyric Acid