Dissecting toxicity of tau and beta-amyloid.
BACKGROUND
How beta-amyloid (Abeta) and tau exert toxicity in Alzheimer's disease is only partly understood. Major questions include (1) which aggregation state of Abeta confers toxicity, (2) do amyloidogenic proteins have similar mechanisms of toxicity, and (3) does soluble tau interfere with cellular functions?


METHODS
To determine Abeta toxicity in P301L mutant tau transgenic mice, mitochondrial function was assessed after insult with monomeric, oligomeric and fibrillar Abeta. Amylin and Abeta toxicity were compared in cortical and hippocampal long-term cultures. To determine tau toxicity, K369I mutant tau mice were established as a model of frontotemporal dementia, analyzed biochemically and compared with human diseased brain.


RESULTS
Oligomeric and fibrillar Abeta42 were both toxic, although to different degrees. Human amylin shared toxicity with Abeta42, an effect not observed for nonamyloidogenic rat amylin. Clinical features of K369I tau mice were caused by aberrant interaction of phosphorylated tau with JIP1, a component of the kinesin transport machinery.


CONCLUSIONS
Our data support the notion of a synergistic action of tau and Abeta pathology on mitochondria. A specific conformation of Abeta42 and human amylin determines toxicity. Finally, trapping of JIP1 by phosphorylated tau in the neuronal soma emerges as a fundamental pathomechanism in neurodegeneration.
Götz
Jürgen
J
Alzheimer's and Parkinson's Disease Laboratory, Brain and Mind Research Institute, University of Sydney, Camperdown, N.S.W., Australia. jgoetz@med.usyd.edu.au
Lim
Yun-An
YA
Ke
Yazi D
YD
Eckert
Anne
A
Ittner
Lars M
LM
D000544:Alzheimer Disease / Q000453:epidemiology / Q000209:etiology* / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000235:genetics / Q000633:toxicity*; D000818:Animals; D006801:Humans; D051379:Mice; D009154:Mutation / Q000235:genetics; D051381:Rats; D016875:tau Proteins / Q000235:genetics / Q000633:toxicity*
D016229:Amyloid beta-Peptides; D016875:tau Proteins