title: Microarray-based gene expression profiling and DNA copy number variation analysis of temporal fossa arachnoid cysts.
abstract: BACKGROUND
  Intracranial arachnoid cysts (AC) are membranous sacs filled with CSF-like fluid that are commonly found in the temporal fossa. The majority of ACs are congenital. Typical symptoms are headache, dizziness, and dyscognition. Little is known about genes that contribute to the formation of the cyst membranes.
  
  
  METHODS
  In order to identify differences in gene expression between normal arachnoid membrane (AM) and cyst membrane, we have performed a high-resolution mRNA microarray analysis. In addition we have screened DNA from AC samples for chromosomal duplications or deletions using DNA microarray-based copy number variation analysis.
  
  
  RESULTS
  The transcriptome consisting of 33096 gene probes showed a near-complete similarity in expression between AC and AM samples. Only nine genes differed in expression between the two tissues: ASGR1, DPEP2, SOX9, SHROOM3, A2BP1, ATP10D, TRIML1, NMU were down regulated, whereas BEND5 was up regulated in the AC samples. Three of the AC samples had unreported human DNA copy number variations, all DNA gains.
  
  
  CONCLUSIONS
  Extending results of previous anatomical studies, the present study has identified a small subset of differentially expressed genes and DNA alterations in arachnoid cysts compared to normal arachnoid membrane.
authors.affiliation: Centre for Medical Genetics and Molecular Medicine, Haukeland University Hospital, Bergen, Norway. mads.aarhus@me.com
authors.forename: Mads
authors.initials: M
authors.lastname: Aarhus
authors.forename: Christian A
authors.initials: CA
authors.lastname: Helland
authors.forename: Morten
authors.initials: M
authors.lastname: Lund-Johansen
authors.forename: Knut
authors.initials: K
authors.lastname: Wester
authors.forename: Per M
authors.initials: PM
authors.lastname: Knappskog