title: Arabidopsis RNA-dependent RNA polymerases and dicer-like proteins in antiviral defense and small interfering RNA biogenesis during Turnip Mosaic Virus infection.
abstract: Plants respond to virus infections by activation of RNA-based silencing, which limits infection at both the single-cell and system levels. Viruses encode RNA silencing suppressor proteins that interfere with this response. Wild-type Arabidopsis thaliana is immune to silencing suppressor (HC-Pro)-deficient Turnip mosaic virus, but immunity was lost in the absence of DICER-LIKE proteins DCL4 and DCL2. Systematic analysis of susceptibility and small RNA formation in Arabidopsis mutants lacking combinations of RNA-dependent RNA polymerase (RDR) and DCL proteins revealed that the vast majority of virus-derived small interfering RNAs (siRNAs) were dependent on DCL4 and RDR1, although full antiviral defense also required DCL2 and RDR6. Among the DCLs, DCL4 was sufficient for antiviral silencing in inoculated leaves, but DCL2 and DCL4 were both involved in silencing in systemic tissues (inflorescences). Basal levels of antiviral RNA silencing and siRNA biogenesis were detected in mutants lacking RDR1, RDR2, and RDR6, indicating an alternate route to form double-stranded RNA that does not depend on the three previously characterized RDR proteins.
authors.affiliation: Center for Genome Research and Biocomputing, Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331.
authors.forename: Hernan
authors.initials: H
authors.lastname: Garcia-Ruiz
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takeda
authors.forename: Elisabeth J
authors.initials: EJ
authors.lastname: Chapman
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Sullivan
authors.forename: Noah
authors.initials: N
authors.lastname: Fahlgren
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Brempelis
authors.forename: James C
authors.initials: JC
authors.lastname: Carrington
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D009154:Mutation; D010942:Plant Viruses / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D043244:Ribonuclease III / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D012321:DNA-Directed RNA Polymerases; D043244:Ribonuclease III