Improving the long-term storage of a mammalian biosensor cell line via genetic engineering.
The unique properties of mammalian cells make them valuable for a variety of applications in medicine, industry, and diagnostics. However, the utility of such cells is restricted due to the difficulty in storing them non-frozen for an extended time and still maintaining their stability and responsiveness. In order to extend the active life span of a mammalian biosensor cell line at room and refrigerated temperatures, we have over expressed genes that are reported to provide protection from apoptosis, stress, or oxidation. We demonstrated that over expression of genes from the extremophile, Artemia franciscana, as well as GADD45beta, extends room-temperature storage of fully active cells 3.5-fold, while over production of several anti-apoptotic proteins extended 4 degrees C storage 2- to 3-fold. Methodologies like these that improve the stability of mammalian-cell-based technologies in the absence of freezers may enable widespread use of these tools in applications that have been considered impractical based solely on limited storage characteristics.
Petrovick
Martha S
MS
MIT Lincoln Laboratory, Lexington, Massachusetts 02420, USA. petrovick@ll.mit.edu <petrovick@ll.mit.edu>
Nargi
Frances E
FE
Towle
Theresa
T
Hogan
Kristine
K
Bohane
Marie
M
Wright
Deborah J
DJ
MacRae
Thomas H
TH
Potts
Malcolm
M
Helm
Richard F
RF
D000818:Animals; D001156:Artemia / Q000235:genetics; D015374:Biosensing Techniques; D002460:Cell Line; D002470:Cell Survival; D015870:Gene Expression; D005818:Genetic Engineering; D013696:Temperature; D013997:Time Factors