title: Enhanced anticancer activity of multi-walled carbon nanotube-methotrexate conjugates using cleavable linkers.
abstract: Methotrexate was tethered to multi-walled carbon nanotubes through different cleavable linkers exploiting the ammonium functionalities introduced by 1,3-dipolar cycloaddition reaction of azomethine ylides to the nanotubes. The new nanobio-hybrid conjugates were internalized into human breast cancer cells and it was shown that the cytotoxic activity was strongly dependent on the presence and type of linker.
authors.affiliation: CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunologie et Chimie Thérapeutiques, 67000 Strasbourg, France.
authors.forename: Cristian
authors.initials: C
authors.lastname: Samorì
authors.forename: Hanene
authors.initials: H
authors.lastname: Ali-Boucetta
authors.forename: Raquel
authors.initials: R
authors.lastname: Sainz
authors.forename: Chang
authors.initials: C
authors.lastname: Guo
authors.forename: Francesca Maria
authors.initials: FM
authors.lastname: Toma
authors.forename: Chiara
authors.initials: C
authors.lastname: Fabbro
authors.forename: Tatiana
authors.initials: T
authors.lastname: da Ros
authors.forename: Maurizio
authors.initials: M
authors.lastname: Prato
authors.forename: Kostas
authors.initials: K
authors.lastname: Kostarelos
authors.forename: Alberto
authors.initials: A
authors.lastname: Bianco
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D001391:Azo Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006801:Humans; D008727:Methotrexate / Q000737:chemistry* / Q000633:toxicity; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D013882:Thiosemicarbazones / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D001391:Azo Compounds; D003432:Cross-Linking Reagents; D037742:Nanotubes, Carbon; D013882:Thiosemicarbazones; C512188:azomethine; D008727:Methotrexate