Strain- and tissue-dependent induction of plasminogen activator inhibitor-1 gene expression in fasted mice.
Plasminogen activator inhibitor-1 (PAI-1), the primary physiological inhibitor of plasminogen activators, is an important contributor to hypofibrinolysis in the presence of metabolic disorders such as diabetes and obesity. The C57BLKS/J (BKS) inbred mouse strain is a popular animal model of type 2 diabetes. We previously described that food deprivation (FD) induces adipose PAI-1 expression in both lean BKS mice and BKS-db/db mice carrying a mutation in the leptin receptor gene. To evaluate the effects of the background of mouse strains, we examined FD-induced PAI-1 expression in the liver, heart and epididymal adipose tissues of BKS, C57BL/6J (B6), C3H/HeN and ICR mice. We found that PAI-1 expression is significantly induced in the heart and liver of fasted mice, although levels of expression in adipose tissues are strain-dependent. The effect of FD on plasma PAI-1 levels is also strain-dependent. Genetic background seems to be an important factor that should be considered when investigating thrombosis and fibrinolysis relative to metabolic changes in mice.
Oishi
Katsutaka
K
Clock Cell Biology Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8566, Japan. k-ooishi@aist.go.jp
Ohkura
Naoki
N
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000378:metabolism*; D005342:Fibrinolysis; D005508:Food Deprivation; D015870:Gene Expression; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics; D018345:Mice, Knockout; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics / Q000378:metabolism*; D054411:Receptors, Leptin / Q000235:genetics; D013927:Thrombosis / Q000378:metabolism*
D017395:Plasminogen Activator Inhibitor 1; D054411:Receptors, Leptin