title: Hsp70 enhances presentation of FMDV antigen to bovine CD4+ T cells in vitro.
abstract: Foot-and-mouth disease virus (FMDV) is the causative agent of a highly contagious acute vesicular disease affecting cloven-hoofed animals, including cattle, sheep and pigs. The current vaccine induces a rapid humoral response, but the duration of the protective antibody response is variable, possibly associated with a variable specific CD4+ T cell response. We investigated the use of heat shock protein 70 (Hsp70) as a molecular chaperone to target viral antigen to the Major Histocompatibility Complex (MHC) class II pathway of antigen presenting cells and generate enhanced MHC II-restricted CD4+ T cell responses in cattle. Monocytes and CD4+ T cells from FMDV vaccinated cattle were stimulated in vitro with complexes of Hsp70 and FMDV peptide, or peptide alone. Hsp70 was found to consistently improve the presentation of a 25-mer FMDV peptide to CD4+ T cells, as measured by T cell proliferation. Complex formation was required for the enhanced effects and Hsp70 alone did not stimulate proliferation. This study provides further evidence that Hsp70:peptide complexes can enhance antigen-specific CD4+ T cell responses in vitro for an important pathogen of livestock.
authors.affiliation: Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.
authors.forename: Kerry
authors.initials: K
authors.lastname: McLaughlin
authors.forename: Julian
authors.initials: J
authors.lastname: Seago
authors.forename: Lucy
authors.initials: L
authors.lastname: Robinson
authors.forename: Charles
authors.initials: C
authors.lastname: Kelly
authors.forename: Bryan
authors.initials: B
authors.lastname: Charleston
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000502:physiology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control / Q000821:virology; D002478:Cells, Cultured; D005536:Foot-and-Mouth Disease / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000494:pharmacology*; D014765:Viral Vaccines
chemical_list: D000956:Antigens, Viral; D018840:HSP70 Heat-Shock Proteins; D014765:Viral Vaccines