title: Hydrazone ligation strategy to assemble multifunctional viral nanoparticles for cell imaging and tumor targeting.
abstract: Multivalent nanoparticle platforms are attractive for biomedical applications because of their improved target specificity, sensitivity, and solubility. However, their controlled assembly remains a considerable challenge. An efficient hydrazone ligation chemistry was applied to the assembly of Cowpea mosaic virus (CPMV) nanoparticles with individually tunable levels of a VEGFR-1 ligand and a fluorescent PEGylated peptide. The nanoparticles recognized VEGFR-1 on endothelial cell lines and VEGFR1-expressing tumor xenografts in mice, validating targeted CPMV as a nanoparticle platform in vivo.
authors.affiliation: Department of Cell Biology.
authors.forename: Florence M
authors.initials: FM
authors.lastname: Brunel
authors.forename: John D
authors.initials: JD
authors.lastname: Lewis
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Destito
authors.forename: Nicole F
authors.initials: NF
authors.lastname: Steinmetz
authors.forename: Marianne
authors.initials: M
authors.lastname: Manchester
authors.forename: Heidi
authors.initials: H
authors.lastname: Stuhlmann
authors.forename: Philip E
authors.initials: PE
authors.lastname: Dawson
mesh_terms: D000818:Animals; D016503:Drug Delivery Systems / Q000379:methods*; D019073:HT29 Cells; D006801:Humans; D006835:Hydrazones / Q000737:chemistry*; D007089:Image Enhancement / Q000379:methods*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D014771:Virion / Q000737:chemistry*
chemical_list: D006835:Hydrazones