A comparison of the VP1, VP2, and VP4 regions for molecular typing of human enteroviruses.
The VP4, VP2, and VP1 gene regions were evaluated for their usefulness in typing human enteroviruses. Three published RT-PCR primers sets targeting separately these three gene regions were used. Initially, from a total of 86 field isolates (36 HEV-A, 40 HEV-B, and 10 HEV-C) tested, 100% concordance in HEV-A was identified from all three gene regions (VP4, VP2, and VP1). However, for HEV-B and HEV-C viruses, only the VP2 and VP1 regions, and not VP4, showed 100% concordance in typing these viruses. To evaluate further the usefulness of VP4 in typing HEV-A enteroviruses, 55 Japanese and 203 published paired VP4 and VP1 nucleotide sequences were also examined. In each case, typing by VP4 was 100% in concordance with typing using VP1. Given these results, it is proposed that for HEV-A enteroviruses, all three gene regions (VP4, VP2, and VP1), would be useful for typing these viruses. These options would enhance the capability of laboratories in identifying these viruses and would greatly help in outbreaks of hand, foot, and mouth disease.
Perera
David
D
Institute of Health & Community Medicine, Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia. davidperera@yahoo.com
Shimizu
Hiroyuki
H
Yoshida
Hiromu
H
Tu
Phan Van
PV
Ishiko
Hiroaki
H
McMinn
Peter C
PC
Cardosa
Mary J
MJ
D017931:DNA Primers / Q000235:genetics; D004770:Enterovirus / Q000145:classification* / Q000235:genetics*; D005838:Genotype; D006801:Humans; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D015678:Viral Structural Proteins / Q000235:genetics*
D017931:DNA Primers; D015678:Viral Structural Proteins