title: Evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corn cob for improved saccharification.
abstract: Ionic liquid (IL) pretreatment of lignocellulose materials is a promising process in biomass conversion to renewable biofuel. More in-depth research involving environment-friendly IL is much needed to explore pretreatment green route. In our case, IL 1-methyl-3-methylimidazolium dimethylphosphite ([Mmim]DMP) was chosen as an environment-friendly solvent to pretreat corn cob in view of its biocompatibility with both lignocellulose solubility and cellulase activity. The pretreatment/saccharification process and in situ saccharification process involving [Mmim]DMP were efficiently performed in bioconversion of corn cob to sugars, and more than 70% saccharification rates were obtained. Furthermore, the fermentability of reducing sugars obtained from the hydrolyzates was evaluated using Rhodococcus opacus strain ACCC41043 (R. opacus). High lipid production 41-43% of cell dry matter was obtained after 30 h of cultivation. GC/MS analysis indicated that lipids from R. opacus contained mainly long-chain fatty acids with four major constituent/oleic acid, stearic acid, palmitic acid, palmitoleic acid which are good candidates for biodiesel. These elucidated that corn cob pretreated by IL [Mmim]DMP did not bring negative effects on saccharification, cell growth, and accumulation of lipid of R. opacus. In conclusion, the IL [Mmim]DMP shows promise as green pretreatment solvent for cellulosic materials.
authors.affiliation: Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Xinglin
authors.initials: X
authors.lastname: Jiang
authors.forename: Yucai
authors.initials: Y
authors.lastname: He
authors.forename: Liangzhi
authors.initials: L
authors.lastname: Li
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
authors.forename: Jianming
authors.initials: J
authors.lastname: Yang
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D006868:Hydrolysis; D007093:Imidazoles / Q000737:chemistry* / Q000494:pharmacology; D052578:Ionic Liquids / Q000737:chemistry* / Q000494:pharmacology; D012240:Rhodococcus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry* / Q000382:microbiology
chemical_list: C516814:1,3-dimethylimidazolium; D005227:Fatty Acids; D007093:Imidazoles; D052578:Ionic Liquids; D002482:Cellulose