title: Newly synthesized poly(glycidyl methacrylate-co-3-thienylmethylmethacrylate)-based electrode designs for phenol biosensors.
abstract: A newly synthesized poly(glycidyl methacrylate-co-3-thienylmethylmethacrylate) [poly(GMA-co-MTM)] was designed to fabricate various HRP electrodes for detection of phenol derivatives. The results showed that the poly(GMA-co-MTM)/polypyrrole composite film microarchitecture provided a good electroactivity as a result of pyrrole and thiophene interaction, and provided chemical bonds for enzyme immobilization via the epoxy groups of poly(GMA-co-MTM). The glassy carbon-based working electrode displayed significantly higher performance for the same composite film configuration comparing to the gold-based working electrode. Poly(GMA-co-MTM)/polypyrrole/HRP coated glassy carbon electrode exhibited a fast response less than 3s, a high sensitivity (200 nA microM(-1)for hydroquinone), a good operational stability (%RSD values ranged between 2 and 5.1 for all phenolics), a long-term stability (retained about 80% of initial activity at the end of 40th day) and a low detection limit ranging between 0.13 and 1.87 microM for the tested.
authors.affiliation: Department of Environmental Engineering, Gebze Institute of Technology, 41400 Gebze, Kocaeli, Turkey.
authors.forename: Seyda Korkut
authors.initials: SK
authors.lastname: Ozoner
authors.forename: Elif
authors.initials: E
authors.lastname: Erhan
authors.forename: Faruk
authors.initials: F
authors.lastname: Yilmaz
authors.forename: Asuman
authors.initials: A
authors.lastname: Celik
authors.forename: Bulent
authors.initials: B
authors.lastname: Keskinler
mesh_terms: D000588:Amines / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D004852:Epoxy Compounds / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D057230:Limit of Detection; D016014:Linear Models; D008768:Methylmethacrylates / Q000138:chemical synthesis* / Q000737:chemistry; D019800:Phenol / Q000032:analysis*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D000588:Amines; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D008768:Methylmethacrylates; D011108:Polymers; C549490:poly(glycidyl methacrylate-co-3-thienylmethylmethacrylate); D019800:Phenol; D006735:Horseradish Peroxidase