title: Efficient transposition of a single Minos transposon copy in the genome of the ascidian Ciona intestinalis with a transgenic line expressing transposase in eggs.
abstract: Transgenesis with transposons is an important technique for studying genetic functions. In the ascidian Ciona intestinalis, methods for germline transformation with the Tc1/mariner transposon Minos have been established. A system to remobilize a single Minos copy in the genome is needed to refine this transgenic technique. In this study, such an experimental system was established with a transgenic line expressing Minos transposase in eggs. In the eggs of a double transgenic animal from a cross between the egg transposase line and a transgenic line having a single Minos insertion, the transposon was transposed into new positions of the Ciona genome, thus creating new insertions. Some of the new insertions caused enhancer detection. The majority of the new insertion sites were mapped on different chromosomes from that of the transposon donor. This characteristic of Minos is in contrast to that of the Sleeping Beauty transposon, which causes frequent intrachromosomal transposition.
authors.affiliation: Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka 415-0025, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Hozumi
authors.forename: Narudo
authors.initials: N
authors.lastname: Kawai
authors.forename: Reiko
authors.initials: R
authors.lastname: Yoshida
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Ogura
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Ohta
authors.forename: Honoo
authors.initials: H
authors.lastname: Satake
authors.forename: Nori
authors.initials: N
authors.lastname: Satoh
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Sasakura
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002938:Ciona intestinalis / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics*; D004526:Efficiency; D004625:Embryo, Nonmammalian; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental; D016678:Genome; D008954:Models, Biological; D016254:Mutagenesis, Insertional / Q000235:genetics / Q000502:physiology; D010063:Ovum / Q000201:enzymology / Q000378:metabolism*; D019895:Transposases / Q000235:genetics / Q000378:metabolism*; D014561:Urochordata / Q000235:genetics / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; C473499:Minos transposase; D019895:Transposases