Antibacterial effects of vulgarone B from Artemisia iwayomogi alone and in combination with oxacillin.
The antibacterial activities of vulgarone B, a component of Artemisia iwayomogi essential oil, were evaluated against some antibiotic-susceptible and -resistant human pathogens. Moreover, the effects of combining antibiotics, such as oxacillin, with vulgarone B were determined in this study. Significant inhibitory activities of Artemisia oils against antibiotic-susceptible and -resistant bacteria were confirmed by broth microdilution methods. The effects of vulgarone B on bacterial morphology and DNA were observed by scanning electron microscope and electrophoresis, respectively. In checkerboard microtiter tests, vulgarone B and A. iwayomogi oil combined with oxacillin resulted in synergism, or additive effects. Moreover, the safety of Artemisia oil and vulgarone B were confirmed in vivo. Both vulgarone B and the essential oil fraction of A. iwayomogi showed significant inhibitory activities against strains of antibioticsusceptible and -resistant bacteria. The oils showed synergism or additive effects when combined with oxacillin against two strains of Staphylococcus aureus. The antibiotic mechanism involved might be related to DNA cleavage. Thus, vulgarone B and the essential oil fraction of A. iwayomogi may be promising candidates for a safe, effective, natural agent active against antibiotic-resistant S. aureus, especially when combined with oxacillin.
Chung
Eun Young
EY
College of Pharmacy, Duksung Women's University, Seoul, 132-714, Korea.
Byun
Youn Hee
YH
Shin
Eun Joo
EJ
Chung
Ha Sook
HS
Lee
Yeon Hee
YH
Shin
Seungwon
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D019306:Artemisia / Q000737:chemistry*; D003470:Culture Media; D004269:DNA, Bacterial / Q000037:antagonists & inhibitors; D004338:Drug Combinations; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D009822:Oils, Volatile / Q000737:chemistry; D010068:Oxacillin / Q000494:pharmacology*; D012477:Salmonella enteritidis / Q000187:drug effects / Q000648:ultrastructure; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D013211:Staphylococcus aureus / Q000187:drug effects / Q000648:ultrastructure
D000900:Anti-Bacterial Agents; D003470:Culture Media; D004269:DNA, Bacterial; D004338:Drug Combinations; D009822:Oils, Volatile; D012717:Sesquiterpenes; C480590:vulgarone B; D010068:Oxacillin