A rapid optical immunoassay for the screening of T-2 and HT-2 toxin in cereals and maize-based baby food.
A rapid surface plasmon resonance (SPR) screening assay has been developed for the combined detection of T-2 and HT-2 toxins in naturally contaminated cereals using a sensor chip coated with an HT-2 toxin derivative and a monoclonal antibody. The antibody raised against HT-2 displayed high cross-reactivity with T-2 toxin while there was no cross-reaction observed with other commonly occurring trichothecenes. A simple extraction procedure using 40% methanol was applied to baby food, breakfast cereal, and wheat samples prior to biosensor analysis. Limits of detection (LOD) for each matrix were determined as 25 microg kg(-1) for baby food and breakfast cereal and 26 microg kg(-1) for wheat. Intra-assay precision (n=6) was calculated for each matrix. The results were expressed as the relative standard deviation and determined as 2.8% (100 microg kg(-1)) and 1.8% (200 microg kg(-1)) in breakfast cereal, 4.6% (50 microg kg(-1)) and 3.6% (100 microg kg(-1)) in wheat and 0.97% (25 microg kg(-1)) and 6.3% (50 microg kg(-1)) in baby food. Between run precision (n=3) performed at the same levels yielded relative standard deviations of 6.7% and 3.9% for breakfast cereals, 3.3% and 1.6% for wheat and 6.8% and 0.08% for baby food, respectively.
Meneely
Julie P
JP
Institute of Agri-food and Land Use, School of Biological Sciences, Queen's University of Belfast, David Keir Building, Stranmillis Road, Belfast BT9 5AG, United Kingdom. j.p.meneely@qub.ac.uk
Sulyok
Michael
M
Baumgartner
Sabine
S
Krska
Rudolf
R
Elliott
Christopher T
CT
D002523:Edible Grain / Q000737:chemistry*; D005504:Food Analysis; D007118:Immunoassay / Q000379:methods*; D007225:Infant Food / Q000032:analysis*; D055620:Optical Phenomena; D015203:Reproducibility of Results; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000276:immunology; D013997:Time Factors; D003313:Zea mays / Q000737:chemistry*
D013605:T-2 Toxin; C012351:HT-2 toxin