Membrane damage and solute leakage from germinating pea seed under cadmium stress.
Seed germination represents a limiting stage of plant life cycle under heavy metal stress situation. Delay in germination can be associated with disorders in the event chain of germinative metabolism which is a highly complex multistage process, but one of underlying metabolic activities following imbibition of seed is the storage mobilization. The influence of cadmium on carbohydrates and aminoacids export from cotyledon to embryonic axis during germination of pea seed was investigated. Compared to the control, Cd caused a restriction in reserve mobilization as evidenced by the pronounced increase in cotyledon/embryo ratios of total soluble sugars, glucose, fructose and aminoacids. Moreover, the nutrient concentrations, as well as the electrical conductivity of germination medium were determined to quantify the extent of solute leakage. Such nutrients were lost into the imbibition medium at the expense of suitable mobilization to the growing embryonic axis. This was concomitant with an over-accumulation of lipid peroxidation products in Cd-poisoned embryonic tissues. However, the impairment of membrane integrity cannot be due to a stimulation in lipoxygenase activity, since the later was markedly inhibited after Cd exposure.
Rahoui
Sondès
S
Bio-Physiologie Cellulaires, Département des Sciences de la Vie, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia.
Chaoui
Abdelilah
A
El Ferjani
Ezzeddine
E
D000596:Amino Acids / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure*; D018548:Cotyledon / Q000254:growth & development; D004553:Electric Conductivity; D018525:Germination; D015227:Lipid Peroxidation; D008084:Lipoxygenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018532:Pisum sativum / Q000378:metabolism*; D012639:Seeds / Q000254:growth & development; D012989:Soil Pollutants / Q000633:toxicity
D000596:Amino Acids; D012989:Soil Pollutants; D002104:Cadmium; D008315:Malondialdehyde; D008084:Lipoxygenase