Patterned transgene expression in multiple-channel bridges after spinal cord injury.
Patterning of gene delivery on sub-millimeter length scales within tissue engineering scaffolds is fundamental to recreating the complex architectures of tissues. Surface-mediated delivery of lipoplexes mixed with fibronectin was investigated to pattern vectors within 250 microm channels in poly(lactide-co-glycolide) (PLG) bridges. Initial studies performed in vitro on PLG surfaces indicated that a DNA density of 0.07 microg mm(-2) inside each channel with a weight ratio of DNA to fibronectin of 1:20 maximized the number of transfected cells and the levels of transgene expression. Patterned vectors encoding for nerve growth factor (NGF) resulted in localized neurite extension within the channel. Translation to three-dimensional multiple-channel bridges enabled patterned transfection of different vectors throughout the channels for DNA:fibronectin ratios of 1:4 and multiple DNA depositions, with a large increase of neural cell bodies and neurite extension for delivery of DNA encoding for NGF. In vivo, the immobilization of non-viral vectors within the channels resulted in localized transfection within the pore structure of the bridge immediately around the channels of the bridge containing DNA. This surface immobilization strategy enables patterned gene delivery in vitro and in vivo on length scales of hundreds of microns and may find utility in strategies aimed at regenerating tissues with complex architectures.
De Laporte
Laura
L
Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road E156, Evanston, IL 60208-3120, USA.
Huang
Alyssa
A
Ducommun
Melissa M
MM
Zelivyanska
Marina L
ML
Aviles
Misael O
MO
Adler
Andrew F
AF
Shea
Lonnie D
LD
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D004247:DNA / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D020007:Implants, Experimental; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011098:Polyglactin 910 / Q000494:pharmacology*; D051381:Rats; D020318:Rats, Long-Evans; D044968:Regenerative Medicine; D013119:Spinal Cord Injuries / Q000235:genetics / Q000628:therapy*; D023822:Tissue Engineering; D014162:Transfection / Q000379:methods*; D019076:Transgenes / Q000235:genetics*
D005353:Fibronectins; D011098:Polyglactin 910; D004247:DNA