title: Development and validation of a sensitive LC-MS method for the determination of tramadol in human plasma and urine.
abstract: A sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS) method was developed and validated for the determination of Tramadol in human plasma and urine. The analyte was separated on a Diamonsil C18 column with ammonium acetate (5 mmol x L(-1))-methanol (50:50,v:v) adjusted PH by caustic soda at a flow rate of 0.8 ml min(-1), and analyzed by mass spectrometry is in positive ion mode. The ion mass spectrum of m/z were 264.1 for Tramadol and 248.0 for Tinidazole (I.S.), respectively. The weighted (1/x2) calibration curve was linear over plasma concentration range 1.00-400.00 ng/ml and urine concentration range 0.01-16.00 microg/ml, with a correlation coefficient (r) of 0.9995 and 0.9997, respectively. The lower limit of quantification in human plasma was 1.00 ng/ml. The inter-and intra-day precisions (CV%) in both plasma and urine were lower than 10%, the mean method accuracies and recoveries from spiked plasma samples at three concentrations ranged from 98.2 to 100.1% and 61.6 to 62.9%, respectively. The developed method was successfully applied to determine Tramadol in human plasma and urine, and provided suitable profiles for clinical pharmacokinetic study of Tramadol.
authors.affiliation: Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Sun
authors.initials: S
authors.lastname: Liang
authors.forename: Ben-Jie
authors.initials: BJ
authors.lastname: Wang
authors.forename: Rui-Chen
authors.initials: RC
authors.lastname: Guo
mesh_terms: D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011398:Promethazine / Q000008:administration & dosage / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D014147:Tramadol / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D000701:Analgesics, Opioid; D004338:Drug Combinations; D014147:Tramadol; D011398:Promethazine