title: Lactic acid bacteria as adjuvants for sublingual allergy vaccines.
abstract: We compared immunomodulatory properties of 11 strains of lactic acid bacteria as well as their capacity to enhance sublingual immunotherapy efficacy in a murine asthma model. Two types of bacterial strains were identified, including: (i) potent inducers of IL-12p70 and IL-10 in dendritic cells, supporting IFN-gamma and IL-10 production in CD4+ T cells such as Lactobacillus helveticus; (ii) pure Th1 inducers such as L. casei. Sublingual administration in ovalbumin-sensitized mice of L. helveticus, but not L. casei, reduced airways hyperresponsiveness, bronchial inflammation and proliferation of specific T cells in cervical lymph nodes. Thus, probiotics acting as a Th1/possibly Treg, but not Th1 adjuvant, potentiate tolerance induction via the sublingual route.
authors.affiliation: Stallergènes SA, Research and Development, 6 rue Alexis de Tocqueville, 92183 Antony, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Van Overtvelt
authors.forename: Helene
authors.initials: H
authors.lastname: Moussu
authors.forename: Stéphane
authors.initials: S
authors.lastname: Horiot
authors.forename: Sandrine
authors.initials: S
authors.lastname: Samson
authors.forename: Vincent
authors.initials: V
authors.lastname: Lombardi
authors.forename: Laurent
authors.initials: L
authors.lastname: Mascarell
authors.forename: Ariane
authors.initials: A
authors.lastname: van de Moer
authors.forename: Raphaëlle
authors.initials: R
authors.lastname: Bourdet-Sicard
authors.forename: Philippe
authors.initials: P
authors.lastname: Moingeon
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000286:Administration, Sublingual; D000485:Allergens / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D007108:Immune Tolerance; D007778:Lactobacillus / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D019936:Probiotics / Q000008:administration & dosage*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014612:Vaccines / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000485:Allergens; D014612:Vaccines; D010047:Ovalbumin