Neuroprotective effects of ischemic preconditioning on global brain ischemia through up-regulation of acid-sensing ion channel 2a.
Transient forebrain or global ischemia induces cell death in vulnerable CA1 pyramidal neurons. A brief period of ischemia, i.e., ischemic preconditioning, affords CA1 neurons robust protection against a subsequent, more prolonged ischemic challenge. Using the four-vessel occlusion model, we established an ischemic preconditioning model in which rodents were subjected to 3 min of sublethal ischemia 48 h before a 15 min lethal ischemia. We showed that preconditioning attenuated the ischemia-induced neural cell death and DNA fragmentation in the hippocampal CA1 region. RT-PCR and western blot analysis showed that preconditioning prior to an ischemic insult significantly increased ASIC 2a mRNA and protein expression in comparison to the ischemic insult alone (p < 0.01). These findings implicate a new role of ASIC 2a on endogenous neuroprotection from ischemic insult.
Miao
Yifeng
Y
Department of Neurosurgery, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, China. yifengmwx@hotmail.com
Zhang
Weiqiao
W
Lin
Yuchang
Y
Lu
Xiaojie
X
Qiu
Yongming
Y
D062565:Acid Sensing Ion Channels / Q000235:genetics / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D056547:CA1 Region, Hippocampal / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D020287:In Situ Nick-End Labeling; D019194:Ischemic Preconditioning; D008297:Male; D018696:Neuroprotective Agents; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015854:Up-Regulation
D062565:Acid Sensing Ion Channels; C568425:Asic2 protein, rat; D018696:Neuroprotective Agents; D012333:RNA, Messenger