title: Development of a novel PCR method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections.
abstract: OBJECTIVE
  The objective of this study was to develop a novel polymerase chain reaction (PCR) method to comprehensively analyze salivary bacterial flora.
  
  
  METHODS
  The bacterial flora in the saliva of 10 healthy persons and 11 patients with odontogenic infections were examined using a DNA extraction method with a high level of cell destruction efficiency and a novel universal primer set to amplify approximately 580 bp of the 16S rDNA sequence.
  
  
  RESULTS
  Streptococcus (54.5%), Neisseria (14.7%), Actinomyces (8.4%), Gemella (4.1%), Granulicatella (3.8%), and Prevotella (1.4%) were dominant in a total of 1655 clones examined from the saliva of the healthy subjects. The dominant genera differed among the patients with odontogenic infections (a total of 823 clones) and were entirely different from those of the healthy subjects.
  
  
  CONCLUSIONS
  This novel comprehensive salivary bacterial flora analysis method may be a useful supportive method to identify causative agents of odontogenic infections.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Saga University, Saga, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Akiyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyamoto
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Fukuda
authors.forename: Naoto
authors.initials: N
authors.lastname: Sano
authors.forename: Nanako
authors.initials: N
authors.lastname: Katagiri
authors.forename: Takeo
authors.initials: T
authors.lastname: Shobuike
authors.forename: Akiko
authors.initials: A
authors.lastname: Kukita
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Hatsumi
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Masaaki
authors.initials: M
authors.lastname: Goto
mesh_terms: D000190:Actinomyces / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001419:Bacteria / Q000145:classification*; D001424:Bacterial Infections / Q000175:diagnosis*; D001431:Bacteriological Techniques; D016866:Bacteroidaceae Infections / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009059:Mouth Diseases / Q000382:microbiology*; D009343:Neisseria / Q000302:isolation & purification; D016870:Neisseriaceae Infections / Q000382:microbiology; D010462:Peptostreptococcus / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D018720:Prevotella / Q000302:isolation & purification; D012463:Saliva / Q000382:microbiology*; D040141:Staphylococcaceae / Q000302:isolation & purification; D013290:Streptococcal Infections / Q000382:microbiology; D013291:Streptococcus / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal