title: Radiolabelled cyanidin 3-O-glucoside is poorly absorbed in the mouse.
abstract: Anthocyanins are natural pigments abundant in various fruits and berries that are involved in the prevention of various chronic diseases. Their low concentrations in plasma and urine are explained in part by their complex chemistry and the formation of still uncharacterised metabolites. The aim of the present study was to follow the distribution of anthocyanins in the body using 14C-labelled cyanidin 3-O-glucoside (Cy3G) fed by gavage to mice. After the administration of 22.2 kBq 14C-Cy3G (0.93 mg), radioactivity was detected in most organs tested over the following 24 h with a peak observed in inner tissues at 3 h. The major fraction of the radioactivity (44.5 %) was found in the faeces collected 24 h after ingestion. At 3 h after oral administration of 141 kBq 14C-Cy3G (4.76 mg), most of the radioactivity (87.9 % of intake) was recovered in the gastrointestinal (GI) tract, especially in the small intestine (50.7 %) and the caecum (23 %). At this time, 3.3 % of the radioactivity was detected in urine. There was minimal accumulation (0.76 %) of radioactivity in tissues outside the GI tract. Distribution of radioactivity varied among organs, with liver, gallbladder and kidneys showing the highest radioactivity. Taken as a whole, these results show that Cy3G is poorly absorbed in the mouse.
authors.affiliation: Laboratoire de Pharmacognosie et Phytothérapie, Faculté de Pharmacie, Université Clermont 1, Clermont-Ferrand, France. catherine.felgines@u-clermont1.fr
authors.forename: Catherine
authors.initials: C
authors.lastname: Felgines
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Krisa
authors.forename: Aurélie
authors.initials: A
authors.lastname: Mauray
authors.forename: Catherine
authors.initials: C
authors.lastname: Besson
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Lamaison
authors.forename: Augustin
authors.initials: A
authors.lastname: Scalbert
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mérillon
authors.forename: Odile
authors.initials: O
authors.lastname: Texier
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000493:pharmacokinetics*; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D005243:Feces / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D005704:Gallbladder / Q000378:metabolism; D005960:Glucosides / Q000493:pharmacokinetics*; D007408:Intestinal Absorption; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000493:pharmacokinetics*; D013194:Staining and Labeling / Q000379:methods; D014018:Tissue Distribution
chemical_list: D000872:Anthocyanins; D002250:Carbon Radioisotopes; D005960:Glucosides; D010936:Plant Extracts; C462279:cyanidin-3-O-beta-glucopyranoside