Bioactive steroidal alkaloids from Sarcococca hookeriana.
Four new 5 alpha-pregnane-type steroidal alkaloids, hookerianamides L(1), M(2), N(3), and O(4), and a known N-formylchonemorphine (5) have been isolated by acid-base extraction of the dichloromethane extract of Sarcococca hookeriana. The structures of all compounds were determined with spectroscopic techniques and by comparison with literature data. All compounds displayed antileishmanial and antibacterial properties. Compounds 1, 4, and 5 were found to be more potent than standard pentamidine (IC (50) = 9.59 microg/mL) with respect to leishmanicidal activity. The minimum inhibitory concentration of most of the compounds against Bacillus subtilis, Streptococcus minor, and Streptococcus ferus was lower than that of the standard ampicillin.
Devkota
Krishna Prasad
KP
Institute of Forestry, Pokhara Campus, Tribhuvan University, Pokhara, Kaski, Nepal. devkotakpd@yahoo.com
Wansi
Jean D
JD
Lenta
Bruno N
BN
Khan
Samreen
S
Choudhary
Muhammad I
MI
Sewald
Norbert
N
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D031234:Buxaceae / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010419:Pentamidine / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011278:Pregnanes / Q000302:isolation & purification / Q000494:pharmacology*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014344:Trypanocidal Agents / Q000302:isolation & purification* / Q000494:pharmacology
D000470:Alkaloids; D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D011278:Pregnanes; D013256:Steroids; D014344:Trypanocidal Agents; C054612:chonemorphine; D010419:Pentamidine; D000667:Ampicillin