Transcriptional changes in Schistosoma mansoni during early schistosomula development and in the presence of erythrocytes.
BACKGROUND
Schistosomes cause more mortality and morbidity than any other human helminth, but control primarily relies on a single drug that kills adult worms. The newly transformed schistosomulum stage is susceptible to the immune response and is a target for vaccine development and rational drug design.


RESULTS
To identify genes which are up-regulated during the maturation of Schistosoma mansoni schistosomula in vitro, we cultured newly transformed parasites for 3 h or 5 days with and without erythrocytes and compared their transcriptional profiles using cDNA microarrays. The most apparent changes were in the up-regulation of genes between 3 h and 5 day schistosomula involved in blood feeding, tegument and cytoskeletal development, cell adhesion, and stress responses. The most highly up-regulated genes included a tegument tetraspanin Sm-tsp-3 (1,600-fold up-regulation), a protein kinase, a novel serine protease and serine protease inhibitor, and intestinal proteases belonging to distinct mechanistic classes. The inclusion of erythrocytes in the culture medium resulted in a general but less pronounced increase in transcriptional activity, with the highest up-regulation of genes involved in iron metabolism, proteolysis, and transport of fatty acids and sugars.


CONCLUSIONS
We have identified the genes that are up-regulated during the first 5 days of schistosomula development in vitro. Using a combination of gene silencing techniques and murine protection studies, some of these highly up-regulated transcripts can be targeted for future development of new vaccines and drugs.
Gobert
Geoffrey N
GN
Division of Infectious Diseases, Queensland Institute of Medical Research, Queensland, Australia.
Tran
Mai H
MH
Moertel
Luke
L
Mulvenna
Jason
J
Jones
Malcolm K
MK
McManus
Donald P
DP
Loukas
Alex
A
D000818:Animals; D004912:Erythrocytes / Q000469:parasitology*; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth; D006801:Humans; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D012550:Schistosoma mansoni / Q000235:genetics* / Q000254:growth & development*; D015854:Up-Regulation