title: Immunohistochemical and scanning electron microscopic comparison of the collagen network constructions between pig, goat and chicken livers.
abstract: The distribution and three-dimensional architecture of collagen fibers were compared between pig, goat and chicken livers. Immunohistochemical staining revealed that collagen type I was identified in the interlobular connective tissue region and intralobular areas in pigs and goats. Type III collagen was also identified in the interlobular connective tissue region and intralobular sinusoidal walls. In the chicken liver, only the circumference region of the vessels was immunostained with collagen type I and III antibodies and the interlobular connective tissue wall could not be distinguished clearly. In the intralobular region, collagen type I antibody immunoreacted around the hepatic cells but collagen type III antibody immunoreacted weakly. In the NaOH macerated specimen, well-developed collagen bundles formed the prominent interlobular walls in pigs. In contrast, the wall in the goat liver comprised a thin layer of the bundles. In the chicken liver, there were no notable collagen septa between lobules. The intralobular collagen construction was quite different between the animals, indicating a fragile collagen fibril networks in pigs, a robust framework in goats and dense fabric-like septa in chickens. These results indicate that the distinct collagen frameworks may contribute to the histological strength of the livers in each of the animal species.
authors.affiliation: Faculty of Agriculture, Kyushu University, Higashi, Fukuoka, Japan. shotaro@agr.kyushu-u.ac.jp
authors.forename: Shotaro
authors.initials: S
authors.lastname: Nishimura
authors.forename: Ayano
authors.initials: A
authors.lastname: Sagara
authors.forename: Ichiro
authors.initials: I
authors.lastname: Oshima
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Ono
authors.forename: Hisao
authors.initials: H
authors.lastname: Iwamoto
authors.forename: Kaoru
authors.initials: K
authors.lastname: Okano
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Miyachi
authors.forename: Shoji
authors.initials: S
authors.lastname: Tabata
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D003094:Collagen / Q000737:chemistry*; D006041:Goats / Q000378:metabolism*; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D013552:Swine / Q000378:metabolism*
chemical_list: D003094:Collagen