title: Budding and direct division of adult human erythrocytes in serum-free cultures.
abstract: This paper documents the budding and division process of human erythrocytes in vitro using time-lapse light microscopy of hanging-drop preparations. The erythrocytes were prepared from normal adult human peripheral blood. Red blood cells showed cytoplasmic budding, segmentation, and direct division with delayed addition of erythropoietin in serum-free Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham. These observations are thought to be useful for developing model of definitive erythropoiesis and simple expansion of human erythrocytes.
authors.affiliation: Laboratory of Electron Microscopy, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan. g-niimi@fujita-hu.ac.jp
authors.forename: Gen
authors.initials: G
authors.lastname: Niimi
authors.forename: Koji
authors.initials: K
authors.lastname: Ohashi
authors.forename: Tomihiko
authors.initials: T
authors.lastname: Ide
authors.forename: Jaime
authors.initials: J
authors.lastname: Pereda
mesh_terms: D002455:Cell Division / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D004920:Erythropoiesis / Q000502:physiology; D004921:Erythropoietin / Q000494:pharmacology; D006801:Humans; D008853:Microscopy / Q000379:methods
chemical_list: D016895:Culture Media, Serum-Free; D004921:Erythropoietin