title: Synthesis and evaluation of a new fluorescent transglycosylase substrate: lipid II-based molecule possessing a dansyl-C20 polyprenyl moiety.
abstract: The preparation of a novel fluorescent lipid II-based substrate for transglycosylases (TGases) is described. This substrate has characteristic structural features including a shorter lipid chain, a fluorophore tag at the end of the lipid chain rather than on the peptide chain, and no labeling with a radioactive atom. This fluorescent substrate is readily utilized in TGase activity assays to characterize TGases and also to evaluate the activities of TGase inhibitors.
authors.affiliation: Department of Chemistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Liu
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Guo
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Chang
authors.forename: Jen-Tsung
authors.initials: JT
authors.lastname: Wang
authors.forename: Hao-Wei
authors.initials: HW
authors.lastname: Shih
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Hsu
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Chen
authors.forename: Shao-Kang
authors.initials: SK
authors.lastname: Chen
authors.forename: Yen-Chih
authors.initials: YC
authors.lastname: Wang
authors.forename: Ting-Jen R
authors.initials: TJ
authors.lastname: Cheng
authors.forename: Che
authors.initials: C
authors.lastname: Ma
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
authors.forename: Jim-Min
authors.initials: JM
authors.lastname: Fang
authors.forename: Wei-Chieh
authors.initials: WC
authors.lastname: Cheng
mesh_terms: D001455:Bambermycins / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005453:Fluorescence; D016695:Glycosyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015394:Molecular Structure; D011106:Polyisoprenyl Phosphates / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014538:Uridine Diphosphate N-Acetylmuramic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D011106:Polyisoprenyl Phosphates; D014538:Uridine Diphosphate N-Acetylmuramic Acid; C069249:muramyl-NAc-(pentapeptide)pyrophosphoryl-undecaprenol; D001455:Bambermycins; C437243:moenomycin A; D016695:Glycosyltransferases