title: Embryonic stem cell-like cells established by culture of adult ovarian cells in mice.
abstract: OBJECTIVE
  To suggest an alternative strategy for deriving histocompatible stems cells without undertaking genetic manipulation.
  
  
  METHODS
  Prospective approach using an animal model.
  
  
  METHODS
  Stem cell and bioevaluation laboratory, Seoul National University.
  
  
  METHODS
  F1 (C57BL6 X DBA2) and outbred (ICR) mice.
  
  
  METHODS
  Ovarian stroma cells of less than 40 mum in diameter were subcultured with fibroblast monolayer, and colony-forming cells were characterized.
  
  
  METHODS
  Stemness, genotype, and imprinted gene methylation.
  
  
  RESULTS
  Two-lines of colony-forming cells were established, which expressed markers specific for embryonic stem cells (ESC) and formed embryoid bodies and teratomas. Complete matching of microsatellite markers with the cell donor strain confirmed their establishment from ovarian tissue, and identification of both homozygotic and heterozygotic chromosomes raised the possibility of their derivation from parthenogenetic oocytes. However, the use of cells smaller than mature oocytes for primary culture, the difference in imprinted gene methylation compared with parthenogenetic ESCs, and failure to establish the ESC-like cells by primary follicle culture collectively suggested the irrelevancy to gametes.
  
  
  CONCLUSIONS
  Coculture of adult ovarian cells with somatic fibroblasts can yield colony-forming cells having ESC-like activity, which may provide an alternative for establishing autologous stem cells from adults that can be obtained without genetic manipulation.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
authors.forename: Seung Pyo
authors.initials: SP
authors.lastname: Gong
authors.forename: Seung Tae
authors.initials: ST
authors.lastname: Lee
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Lee
authors.forename: Dae Yong
authors.initials: DY
authors.lastname: Kim
authors.forename: Gene
authors.initials: G
authors.lastname: Lee
authors.forename: Sung Gil
authors.initials: SG
authors.lastname: Chi
authors.forename: Byung-Kyu
authors.initials: BK
authors.lastname: Ryu
authors.forename: Chae Hyun
authors.initials: CH
authors.lastname: Lee
authors.forename: Kyung Eun
authors.initials: KE
authors.lastname: Yum
authors.forename: Ho-Joon
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Han
authors.forename: Jonathan L
authors.initials: JL
authors.lastname: Tilly
authors.forename: Jeong Mook
authors.initials: JM
authors.lastname: Lim
mesh_terms: D000818:Animals; D018920:Coculture Techniques; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000096:biosynthesis; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D018398:Homeodomain Proteins / Q000096:biosynthesis; D051379:Mice; D008813:Mice, Inbred ICR; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis; D006080:Ovarian Follicle / Q000166:cytology*; D010312:Parthenogenesis; D013234:Stem Cells / Q000166:cytology; D017154:Stromal Cells / Q000166:cytology; D019098:Telomerase / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis; D000097584:DNA Methyltransferase 3B
chemical_list: D004248:DNA (Cytosine-5-)-Methyltransferases; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D019098:Telomerase; D014157:Transcription Factors; D000097584:DNA Methyltransferase 3B; C475540:Nanog protein, mouse; C513672:Zfp42 protein, mouse; C509187:Tert protein, mouse