title: Gene expression profile of human skeletal muscle and adipose tissue of Chinese Han patients with type 2 diabetes mellitus.
abstract: OBJECTIVE
  To study the differential patterns of gene expression in skeletal muscle and adipose tissue between type 2 diabetes mellitus (T2DM) patients and healthy subjects using DNA microarray analysis.
  
  
  METHODS
  T2DM patiens were divided into female group, young male group and old male group. DNA microarray analysis and quantitative real-time PCR were carried out to analyze the relation between gene expressions and T2DM.
  
  
  RESULTS
  The mRNA expression of 298, 578, and 350 genes was changed in the skeletal muscle of diabetes mellitus patients compared with control subjects. The 1320, 1143, and 2847 genes were modified in adipose tissue of the three groups. Among the genes surveyed, the change of 25 and 39 gene transcripts in skeletal muscle and adipose tissue was > or = 2 folds. These differentially expressed genes were classified into 15 categories according to their functions.
  
  
  CONCLUSIONS
  New genes are found and T2DM can be prevented or cured.
authors.affiliation: Department of Internal Medicine, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Yang
authors.forename: Ruo-Lan
authors.initials: RL
authors.lastname: Xiang
authors.forename: Chang
authors.initials: C
authors.lastname: Yang
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Liu
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Shen
authors.forename: Jin
authors.initials: J
authors.lastname: Zuo
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Chang
authors.forename: Fu-De
authors.initials: FD
authors.lastname: Fang
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000368:Aged; D044466:Asian People; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis