title: An induction effect of heat shock on the transcript of globular acetylcholinesterase in NG108-15 cells.
abstract: Heat shock response, an induced transcription of a set of genes in response to high temperature, occurs in all organisms. In neurons, the catalytic subunit of acetylcholinesterase (AChE(T)) interacts with proline-rich membrane anchor (PRiMA) to form a globular tetrameric form (G(4) form). In this study, we examined the effects of heat shock on the transcription and protein assembly of AChE(T) in cultured NG108-15 cells. The transcription of AChE(T) was rapidly induced by heat shock at 40 degrees C, reaching a 15-fold increase in 3h and decreasing thereafter. On the other hand, the level of PRiMA mRNA was not affected after the heat shock. In parallel with AChE(T) mRNA, the enzymatic activity of cellular AChE, in terms of G(1) and G(2) forms, was increased after heat shock; however, the PRiMA-linked G(4) remained unchanged. These results suggest that heat shock can induce the expression level of AChE(T) by the regulation of AChE(T) transcripts in NG108-15 cells.
authors.affiliation: Department of Biology and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Kowloon, Hong Kong SAR, China. pingchen@ust.hk
authors.forename: Vicky P
authors.initials: VP
authors.lastname: Chen
authors.forename: Heidi Q
authors.initials: HQ
authors.lastname: Xie
authors.forename: Wallace K B
authors.initials: WK
authors.lastname: Chan
authors.forename: K Wing
authors.initials: KW
authors.lastname: Leung
authors.forename: Roy C Y
authors.initials: RC
authors.lastname: Choi
authors.forename: Karl W K
authors.initials: KW
authors.lastname: Tsim
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D055162:Biocatalysis; D045744:Cell Line, Tumor; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015533:Transcriptional Activation
chemical_list: D008565:Membrane Proteins; D021122:Protein Subunits; D012333:RNA, Messenger; D000110:Acetylcholinesterase