PtAAP11, a high affinity amino acid transporter specifically expressed in differentiating xylem cells of poplar.
Amino acids are the currency of nitrogen exchange between source and sink tissues in plants and constitute a major source of the components used for cellular growth and differentiation. The characterization of a new amino acid transporter belonging to the amino acid permease (AAP) family, AAP11, expressed in the perennial species Populus trichocarpa is reported here. PtAAP11 expression analysis was performed by semi-quantitative RT-PCR and GUS activity after poplar transformation. PtAAP11 function was studied in detail by heterologous expression in yeast. The poplar genome contains 14 putative AAPs which is quite similar to other species analysed except Arabidopsis. PtAAP11 was mostly expressed in differentiating xylem cells in different organs. Functional characterization demonstrated that PtAAP11 was a high affinity amino acid transporter, more particularly for proline. Compared with other plant amino acid transporters, PtAAP11 represents a novel high-affinity system for proline. Thus, the functional characterization and expression studies suggest that PtAAP11 may play a major role in xylogenesis by providing proline required for xylem cell wall proteins. The present study provides important information highlighting the role of a specific amino acid transporter in xylogenesis in poplar.
Couturier
Jérémy
J
UMR INRA/UHP 1136 Interactions Arbres-Microorganismes, IFR 110 Ecosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation, Nancy University, Faculté des Sciences et Techniques, BP 70239, F-54506 Vandoeuvre-les-Nancy Cedex, France. Jeremy.Couturier@scbiol.uhp-nancy.fr
de Faÿ
Elisabeth
E
Fitz
Michael
M
Wipf
Daniel
D
Blaudez
Damien
D
Chalot
Michel
M
D026905:Amino Acid Transport Systems / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D010802:Phylogeny; D032107:Populus / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013379:Substrate Specificity; D052584:Xylem / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
D026905:Amino Acid Transport Systems