Radioprotective effect of hydrogen in cultured cells and mice.
It has been demonstrated that hydrogen can selectively reduce hydroxyl and peroxynitrite in vitro. Since most of the ionizing radiation-induced cellular damage is caused by hydroxyl radicals, this study was designed to test the hypothesis that hydrogen may be an effective radioprotective agent. This paper demonstrates that treating cells with hydrogen before irradiation could significantly inhibit ionizing irradiation(IR)-induced Human Lymphocyte AHH-1 cells apoptosis and increase cells viability in vitro. This paper also shows that hydrogen can protect gastrointestinal endothelia from radiation-induced injury, decrease plasma malondialdehyde (MDA) intestinal 8-hydroxydeoxyguanosine (8-OHDG) levels and increase plasma endogenous antioxidants in vivo. It is suggested that hydrogen has a potential as an effective and safe radioprotective agent.
Qian
Liren
L
Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, PR China.
Cao
Fei
F
Cui
Jianguo
J
Huang
Yuecheng
Y
Zhou
Xiaojian
X
Liu
Shulin
S
Cai
Jianming
J
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D006859:Hydrogen / Q000494:pharmacology*; D007422:Intestines / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology*
D011837:Radiation-Protective Agents; D006859:Hydrogen