A microfluidic approach for high-throughput droplet interface bilayer (DIB) formation.
We present a simple, automated method for high-throughput formation of droplet interface bilayers (DIBs) in a microfluidic device. We can form complex DIB networks that are able to fill predefined three dimensional architectures. Moreover, we demonstrate the flexibility of the system by using a variety of lipids including 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC).
Stanley
C E
CE
Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London, UKSW7 2AZ.
Elvira
K S
KS
Niu
X Z
XZ
Gee
A D
AD
Ces
O
O
Edel
J B
JB
Demello
A J
AJ
D019793:Fluorescein / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D009821:Oils / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D014867:Water / Q000737:chemistry
D008051:Lipid Bilayers; D009821:Oils; D010713:Phosphatidylcholines; D014867:Water; C021016:1,2-diphytanoylphosphatidylcholine; C017251:1,2-oleoylphosphatidylcholine; D019793:Fluorescein