title: Bioluminescence imaging of c-fos gene expression accompanying filial imprinting in the newly hatched chick brain.
abstract: Bioluminescence imaging is a powerful tool for examining gene expression in living animals. Previously, we reported that exogenous DNA could be successfully delivered into neurons in the newly hatched chick brain using electroporation. Here, we show the in vivo bioluminescence imaging of c-fos promoter activity and its upregulation, which is associated with filial imprinting. The upregulation of c-fos gene expression correlated with both the strength of the chicks' approach activity to the training object and the acquisition of memory. The present technique should be a powerful tool for analyzing the time changes in neural activity of certain brain areas in real-time during memory formation, using brains of living animals.
authors.affiliation: Faculty of Pharmaceutical Sciences, Teikyo University, Sagamihara, Kanagawa 229-0195, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Eiji
authors.initials: E
authors.lastname: Iikubo
authors.forename: Naoki
authors.initials: N
authors.lastname: Hirose
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kitajima
authors.forename: Sachiko
authors.initials: S
authors.lastname: Katagiri
authors.forename: Ai
authors.initials: A
authors.lastname: Kawamori
authors.forename: Ikuko
authors.initials: I
authors.lastname: Fujii-Taira
authors.forename: Toshiya
authors.initials: T
authors.lastname: Matsushima
authors.forename: Koichi J
authors.initials: KJ
authors.lastname: Homma
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002645:Chickens; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D051766:Early Growth Response Protein 1 / Q000235:genetics / Q000378:metabolism; D018274:Electroporation / Q000379:methods; D015870:Gene Expression / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007173:Imprinting, Psychological / Q000502:physiology; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D013223:Statistics as Topic
chemical_list: D003598:Cytoskeletal Proteins; D051766:Early Growth Response Protein 1; D008164:Luminescent Proteins; D016760:Proto-Oncogene Proteins c-fos