Angiotensin I converting enzyme inhibitory peptide extracted from freshwater zooplankton.
In this study, hydrolysates obtained from the freshwater rotifer Brachionus calyciflonus were investigated for angiotensin I converting enzyme (ACE) inhibitory peptides. Freshwater rotifer protein was hydrolyzed using six separate enzymes in a batch reactor. The peptic hydrolysate had the highest ACE inhibitory activity compared to the other hydrolysates. The highest ACE inhibitory peptide was separated using Sephadex G-25 column chromatography and high-performance liquid chromatography on a C18 column. The 50% inhibitory concentration (IC(50)) value of purified ACE inhibitory peptide was 40.01 microg/mL. ACE inhibitory peptide was identified as being seven amino acid residues of Ala-Gln-Gly-Glu-Arg-His-Arg by N-terminal amino acid sequence analysis. The IC(50) value of purified ACE inhibitory peptide was 47.1 microM, and Lineweaver-Burk plots suggested that the peptide purified from rotifer protein acts as a competitive inhibitor against ACE. The results of this study suggest that peptides derived from freshwater rotifers may be beneficial as antihypertension compounds in functional foods or as pharmaceuticals.
Lee
Jung Kwon
JK
Faculty of Marine Bioscience and Technology, Gangnueng-Wonju National University, Gangneung, Republic of Korea.
Lee
Min-Su
MS
Park
Heum Gi
HG
Kim
Se-Kwon
SK
Byun
Hee-Guk
HG
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001667:Binding, Competitive; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology*; D002845:Chromatography; D005618:Fresh Water; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*; D015048:Zooplankton / Q000737:chemistry*
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D001685:Biological Factors; D010455:Peptides; D011506:Proteins