title: Grafting of antibodies inside integrated microfluidic-microoptic devices by means of automated microcontact printing.
abstract: A novel approach to integrating biochip and microfluidic devices is reported in which microcontact printing is a key fabrication technique. The process is performed using an automated microcontact printer that has been developed as an application-specific tool. As proof-of-concept the instrument is used to consecutively and selectively graft patterns of antibodies at the bottom of a glass channel for use in microfluidic immunoassays. Importantly, feature collapse due to over compression of the PDMS stamp is avoided by fine control of the stamp's compression during contact. The precise alignment of biomolecules at the intersection of microfluidic channel and integrated optical waveguides has been achieved, with antigen detection performed via fluorescence excitation. Thus, it has been demonstrated that this technology permits sequential microcontact printing of isolated features consisting of functional biomolecules at any position along a microfluidic channel and also that it is possible to precisely align these features with existing components.
authors.affiliation: Institut des Nanotechnologies de Lyon, INL UMR CNRS ECL-INSA-UCBL 5270, Ecole Centrale de Lyon, 36 avenue Guy de Collongue, F69134 Ecully, France.
authors.forename: Elie
authors.initials: E
authors.lastname: Bou Chakra
authors.forename: Benjamin
authors.initials: B
authors.lastname: Hannes
authors.forename: Julien
authors.initials: J
authors.lastname: Vieillard
authors.forename: Colin D
authors.initials: CD
authors.lastname: Mansfield
authors.forename: Radoslav
authors.initials: R
authors.lastname: Mazurczyk
authors.forename: Aude
authors.initials: A
authors.lastname: Bouchard
authors.forename: Jan
authors.initials: J
authors.lastname: Potempa
authors.forename: Stanislas
authors.initials: S
authors.lastname: Krawczyk
authors.forename: Michel
authors.initials: M
authors.lastname: Cabrera