Supramolecular hydrogels exhibiting fast in situ gel forming and adjustable degradation properties.
Fast in situ forming supramolecular hydrogels consisted of the tyramine-conjugated supramolecular structures and chitosan derivative were prepared via an enzymatic reaction with horseradish peroxidase (HRP) and hydrogen peroxide (H(2)O(2)). The gel formation was varied within a time period of 5 s to 10 min by controlling the concentrations of HRP, H(2)O(2), and polymers. Tyramine conjugation at different sites of the supramolecular structure resulted in significant changes in physical properties and the degradation time of the hydrogels that were confirmed by water uptake, compressive strength and degradation tests. In addition, the hydrogels showed a good cytocompatibility in vitro. These hydrogels could be promising injectable biomaterials with adjustable degradation times to control both the cellular behaviors as a regenerative cell matrix and the drug release behavior as a drug delivery vehicle.
Tran
Ngoc Quyen
NQ
Department of Molecular Science and Technology, Ajou University, 5 Wonchon, Yeoungtong, Suwon 443-749, Republic of Korea.
Joung
Yoon Ki
YK
Lih
Eugene
E
Park
Kyung Min
KM
Park
Ki Dong
KD
D000818:Animals; D048271:Chitosan / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D020100:Hydrogels; D006861:Hydrogen Peroxide / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D014439:Tyramine / Q000737:chemistry; D014961:X-Ray Diffraction
D020100:Hydrogels; D048271:Chitosan; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D014439:Tyramine