title: Sputum smear microscopy in DOTS: Are three samples necessary? An analysis and its implications in tuberculosis control.
abstract: OBJECTIVE
  To assess relevance of spot morning spot (SMS) method of smear microscopy in tuberculosis control by directly observed treatment short course (DOTS).
  
  
  METHODS
  Screening of smear microscopy results of 546 cases of pulmonary tuberculosis at our DOTS centre.
  
  
  RESULTS
  Among 546 cases, 82% had all three samples positive for AFB, 98% had either morning sample or spot and morning sample positive. 2% had second spot sample positive.
  
  
  CONCLUSIONS
  Examination of third sample, especially second spot sample, does not add significantly to the diagnostic yield. Examination of one spot and early morning samples were able to correctly diagnose 98% cases. This has strong implications in DOTS strategies.
authors.affiliation: Department of Tuberculosis and Respiratory Diseases, Yenepoya Medical College, Deralakatte, Mangalore - 575 018, Karnataka, India.
authors.forename: Sukhesh
authors.initials: S
authors.lastname: Rao