title: Detection of high risk human papillomavirus cervical infections by the hybrid capture in Asunción, Paraguay.
abstract: Cervical cancer is the most frequent malignant tumour of women in Latin America being human papillomavirus (HPV) the main cause. The aim of this study was to increase the knowledge about the cervical infections with oncogenic HPV types (HR-HPV) in Asuncion, Paraguay. Two hundred and seventy-two cervical samples were analyzed using hybrid capture II assay (HCA II) for HR-HPV. The frequency of HR-HPV in the study group was 44%. HR-HPV was detected in 25% of the women negative for squamous intraepithelial lesions (NSIL), 72% with atypical squamous cells of undetermined significance (ASCUS), 68% with low SIL and 78% with high SIL. A moderate concordance was observed between HCA II assay and cytology (kappa: 0.43 IC(95% 0.3-0.5)). It was detected a high frequency of HR-HPV in women from 11 to 30 years old and in those over 60 years old. The data obtained in this study showed a high frequency of HR-HPV in woman with NSIL and ASCUS, which corroborate that the use of cytology together with HCA II assay for HR-HPV could improve remarkably the efficiency of screening programs of cervical cancer in Paraguay. Furthermore, these findings point out the need for the periodical follow-up of HR-HPV infections in older women.
authors.affiliation: Deparment of Public Health and Epidemiology, Research Institute of Health Science, University of Asunción, Asunción, Paraguay. msclauramt@yahoo.com.br
authors.forename: Laura Mendoza
authors.initials: LM
authors.lastname: Torres
authors.forename: Malvina
authors.initials: M
authors.lastname: Páez
authors.forename: Ariel
authors.initials: A
authors.lastname: Insaurralde
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Rodriguez
authors.forename: Amalia
authors.initials: A
authors.lastname: Castro
authors.forename: Elena
authors.initials: E
authors.lastname: Kasamatsu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D010239:Paraguay; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000821:virology; D014626:Vaginal Smears; D019562:Viral Load; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000821:virology
chemical_list: D004279:DNA, Viral