Identification of herpes simplex virus type 1 proteins encoded within the first 1.5 kb of the latency-associated transcript.
Expression of the first 1.5 kb of the latency-associated transcript (LAT) that is encoded by herpes simplex virus type 1 (HSV-1) is sufficient for wild-type (wt) levels of reactivation from latency in small animal models. Peptide-specific immunoglobulin G (IgG) was generated against open reading frames (ORFs) that are located within the first 1.5 kb of LAT coding sequences. Cells stably transfected with LAT or trigeminal ganglionic neurons of mice infected with a LAT expressing virus appeared to express the L2 or L8 ORF. Only L2 ORF expression was readily detected in trigeminal ganglionic neurons of latently infected mice.
Henderson
Gail
G
Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, University of Nebraska, Lincoln, Nebraska 68503, USA.
Jaber
Tareq
T
Carpenter
Dale
D
Wechsler
Steven L
SL
Jones
Clinton
C
D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D020803:Encephalitis, Herpes Simplex / Q000821:virology*; D005260:Female; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000254:growth & development; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009474:Neurons / Q000502:physiology / Q000821:virology*; D016366:Open Reading Frames / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D014162:Transfection; D012668:Trigeminal Ganglion / Q000166:cytology; D014764:Viral Proteins / Q000235:genetics; D017735:Virus Latency / Q000235:genetics*
D035683:MicroRNAs; D014764:Viral Proteins; C477253:latency associated transcript, herpes simplex virus-1