Identification of Candida albicans exposed surface proteins in vivo by a rapid proteomic approach.
We have set up a fast and easy methodology to identify cell-surface proteins in live yeasts. A non-gel proteomic approach was based on a short period of trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS. Candida albicans was used as a model organism and proteins involved in cell wall organization, cell rescue, defense, virulence, transport, protein fate and metabolism were identified. This strategy is a powerful tool to study host-pathogen interactions and to look for potential vaccine candidates and drug targets.
Hernáez
María Luisa
ML
Unidad de Proteómica, Universidad Complutense de Madrid-Parque Científico de Madrid (UCM-PCM), Spain. mlhernae@farm.ucm.es
Ximénez-Embún
Pilar
P
Martínez-Gomariz
Montserrat
M
Gutiérrez-Blázquez
María Dolores
MD
Nombela
César
C
Gil
Concha
C
D002176:Candida albicans / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002474:Cell-Free System / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D005656:Fungal Proteins / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D020543:Proteome / Q000032:analysis*
D005656:Fungal Proteins; D020543:Proteome