Expression and immunolocalization of calcium transport proteins in the canine duodenum, kidney, and pancreas.
The aim of the study was to elucidate expression and localization of several calcium handling proteins and the regulating vitamin D receptor (VDR) in the dog using RT-PCR and immunohistochemistry. The results of RNA expression and antibody staining were compared to ensure specificity. We found several antibodies to be useful for further studies on calcium homeostasis.
Palm
Claudia
C
Clinic of Small Animal Medicine, LMU University of Munich, Muenchen, Germany.
Hartmann
Katrin
K
Weber
Karin
K
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D064026:Calbindins; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D004386:Duodenum / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064030:S100 Calcium Binding Protein G / Q000235:genetics / Q000378:metabolism; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism; D019831:Sodium-Calcium Exchanger / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism
D000906:Antibodies; D064026:Calbindins; D015220:Calcium Channels; D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D064030:S100 Calcium Binding Protein G; D019831:Sodium-Calcium Exchanger; D050916:TRPV Cation Channels; C120776:TRPV6 channel; C110374:sodium-calcium exchanger 1; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases; D002118:Calcium