High-fat diet stimulates IL-1 type I receptor-mediated inflammatory signaling in the skeletal muscle of mice.
Recently, substantial attention has been focused on the association between obesity and chronic inflammation. The aim of the present study was to investigate whether high-fat diet (HFD)-induced obesity induces the activation of the IL-1 type I receptor (IL-1RI)-mediated inflammatory signaling cascade in the skeletal muscle of mice. Male C57BL/6J mice were fed either an HFD or a normal diet (ND) for 12 wk. Compared with the results in mice receiving the ND, the HFD increased the expression of IL-1RI and downstream signaling proteins, such as myeloid differentiation primary response gene 88 (MyD88), IL-1R-associated kinase 4 and phospho-transforming growth factor-activated kinase 1 in the skeletal muscle. Additionally, activities of both inhibitor of kappaB kinase beta and inhibitor of kappaB degradation were significantly elevated in the skeletal muscle of mice fed with an HFD compared with mice receiving an ND. In contrast, the levels of other downstream transcription factors, such as activator protein-1 and INF regulatory factor 5, were not affected by the HFD. These results suggest that the IL-1RI-MyD88-nuclear factor kappaB signaling pathway might be involved in the induction of the inflammatory response in the skeletal muscle of mice fed with an HFD.
Kim
Seung-Jin
SJ
Department of Food and Nutrition, Brain Korea 21 Project, Yonsei University, Seoul 120-749, Korea.
Choi
Youngshim
Y
Jun
Hye-Seung
HS
Kim
Bo-Min
BM
Na
Hye-Kyung
HK
Surh
Young-Jun
YJ
Park
Taesun
T
D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage*; D005786:Gene Expression Regulation; D051550:I-kappa B Kinase / Q000378:metabolism; D020672:I-kappa B Proteins / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism*; D053592:Interleukin-1 Receptor-Associated Kinases / Q000378:metabolism; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D009765:Obesity / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D053573:Receptors, Interleukin-1 Type I / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D000099151:MAP Kinase Kinase Kinase 7
D004041:Dietary Fats; D051550:I-kappa B Kinase; D020672:I-kappa B Proteins; D053592:Interleukin-1 Receptor-Associated Kinases; D020930:MAP Kinase Kinase Kinases; D053594:Myeloid Differentiation Factor 88; D012333:RNA, Messenger; D053573:Receptors, Interleukin-1 Type I; D000099151:MAP Kinase Kinase Kinase 7; C551354:IkappaBeta protein, mouse; C507780:Myd88 protein, mouse; C507774:Irak4 protein, mouse; C496562:Ikbkb protein, mouse