title: Evaluation of the tissue damage of porcine ovaries after bipolar drilling under transvaginal hydrolaparoscopy--an in vitro experiment.
abstract: OBJECTIVE
  To evaluate the tissue damage of in vitro porcine ovarian drilling in saline solution mimicking transvaginal hydrolaparoscopy (THL).
  
  
  METHODS
  In vitro ovarian drilling with different current and activation time was applied on fresh porcine ovaries, 15 ovaries in each group and 2 punctures in each ovary. Drilling using 5-Fr bipolar needle was performed in saline solution mimicking THL, while saline solution rinsing the ovaries after monopolar electrocoagulation.
  
  
  RESULTS
  The monopolar drilling caused more tissue damage than the bipolar needle in saline (p < 0.01). The ratio of the damage of monopolar electrocoagulation (40 w, 3s) over that of bipolar diathermy in saline solution (70 w, 15 s) was 7.4 [(16.74 +/- 1.30) mm(3)/(2.27 +/- 0.49) mm(3)]. In the bipolar groups, the 70 w power set (15s and 20 s) caused significantly more tissue damage than the 50 w ones (p < 0.05), and the amount of damage was not stimulation time dependent (p > 0.05).
  
  
  CONCLUSIONS
  The tissue damage caused by monopolar electrocoagulation with 40 w for 3 s was about seven times of that caused by 5-Fr bipolar one in saline with 70 w for 15 s . In THL drilling using a 5-Fr bipolar electrode, the current is more crucial than stimulation time.
authors.affiliation: Center for Reproductive Medicine, Peking University Third Hospital, Beijing, China.
authors.forename: Cai-Hong
authors.initials: CH
authors.lastname: Ma
authors.forename: Shuo
authors.initials: S
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004564:Electrocoagulation / Q000379:methods*; D005260:Female; D010535:Laparoscopy / Q000379:methods*; D009339:Needles; D010053:Ovary / Q000601:surgery*; D013552:Swine