title: Histamine modified 2'-deoxyriboadenosine--potential copper binding site in DNAzymes.
abstract: Copper(II) complexes of histamine modified 2'-deoxyriboadenosine (N-[(9-beta-D-2'-deoxyribofuranosylpurin-6-yl)-carbamoyl]histamine) ligand were studied by potentiometric, UV-visible and EPR techniques. The imidazole residue of the ligand was described as the main binding site forming mono-, bis-(ligand) and dimer complexes, but the interactions between adenosine nitrogen N(1) and carbamoyl nitrogen atoms and the copper(II) ion also were detected. This is the first report evaluating the coordinating ability of such a modified adenosine ligand towards copper(II) ion. Our findings suggest that histamine modified 2'-deoxyriboadenosine could chelate efficiently copper(II) ions if it were incorporated into DNAzyme sequence.
authors.affiliation: Institute of General Food Chemistry, Technical University of Łódź, ul. Stefanowskiego 4/10, 90-924 Łódź, Poland. elalodyg@p.lodz.pl <elalodyg@p.lodz.pl>
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Łodyga-Chruścińska
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Sochacka
authors.forename: Damian
authors.initials: D
authors.lastname: Smuga
authors.forename: Longin
authors.initials: L
authors.lastname: Chruściński
authors.forename: Giovanni
authors.initials: G
authors.lastname: Micera
authors.forename: Daniele
authors.initials: D
authors.lastname: Sanna
authors.forename: Monika
authors.initials: M
authors.lastname: Turek
authors.forename: Monika
authors.initials: M
authors.lastname: Gasiorkiewicz
mesh_terms: D000241:Adenosine / Q000737:chemistry*; D001665:Binding Sites; D003300:Copper / Q000737:chemistry*; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D006632:Histamine / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D015394:Molecular Structure; D011199:Potentiometry; D011522:Protons; D013056:Spectrophotometry, Ultraviolet
chemical_list: D021881:DNA, Catalytic; D008024:Ligands; D011522:Protons; D003300:Copper; D006632:Histamine; D000241:Adenosine