title: Distinguishing ovarian maturity of farmed white sturgeon (Acipenser transmontanus) by Fourier transform infrared spectroscopy: a potential tool for caviar production management.
abstract: Fourier transform infrared spectroscopy (FT-IR, 4000-400 cm(-1)) was applied to blood plasma of farmed white sturgeon (N = 40) to differentiate and predict the stages of ovarian maturity. Spectral features of sex steroids (approximately 3000 cm(-1)) and vitellogenin (approximately 1080 cm(-1)) were identified. Clear segregation of maturity stages (previtellogenesis, vitellogenesis, postvitellogenesis, and follicular atresia) was achieved using principal component analysis (PCA). Progression of oocyte development in the late phase of vitellogenesis was also monitored using PCA based on changes in plasma concentrations of sex steroid and lipid content. The observed oocyte polarization index (PI, a measure of nuclear migration) was correlated with changes in plasma sex steroid levels revealed by FT-IR PCA results. A partial least squares (PLS) model predicted PI values within the range 0.12-0.40 (R = 0.95, SEP = 2.18%) from differences in spectral features. These results suggest that FT-IR may be a good tool for assessing ovarian maturity in farmed sturgeon and will reduce the need for the invasive ovarian biopsy required for PI determination.
authors.affiliation: School of Food Science, Washington State University, Pullman, Washington 99163, USA.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Lu
authors.forename: Molly
authors.initials: M
authors.lastname: Webb
authors.forename: Mariah
authors.initials: M
authors.lastname: Talbott
authors.forename: Joel
authors.initials: J
authors.lastname: Van Eenennaam
authors.forename: Amanda
authors.initials: A
authors.lastname: Palumbo
authors.forename: Javier
authors.initials: J
authors.lastname: Linares-Casenave
authors.forename: Serge
authors.initials: S
authors.lastname: Doroshov
authors.forename: Peter
authors.initials: P
authors.lastname: Struffenegger
authors.forename: Barbara
authors.initials: B
authors.lastname: Rasco
mesh_terms: D000818:Animals; D005260:Female; D005399:Fishes; D009865:Oocytes / Q000737:chemistry / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D010053:Ovary / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D017747:Seafood / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014818:Vitellogenesis; D014819:Vitellogenins / Q000378:metabolism
chemical_list: D014819:Vitellogenins