Light-induced local heating for thermophoretic manipulation of DNA in polymer micro- and nanochannels.
We present a method for making polymer chips with a narrow-band near-infrared absorber layer that enables light-induced local heating of liquids inside fluidic micro- and nanochannels fabricated by thermal imprint in polymethyl methacrylate. We have characterized the resulting liquid temperature profiles in microchannels using the temperature dependent fluorescence of the complex [Ru(bpy)(3)](2+). We demonstrate thermophoretic manipulation of individual YOYO-1 stained T4 DNA molecules inside micro- and nanochannels.
Thamdrup
Lasse H
LH
Department of Micro- and Nanotechnology, Technical University of Denmark, DK-2800 Lyngby, Denmark.
Larsen
Niels B
NB
Kristensen
Anders
A
D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D004586:Electrophoresis / Q000379:methods*; D006361:Heating / Q000379:methods*; D008027:Light; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008846:Micromanipulation / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods; D011108:Polymers / Q000737:chemistry
D011108:Polymers; D004247:DNA