title: New efficient recombinant expression system to engineer Candida antarctica lipase B.
abstract: Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme.
authors.affiliation: Ingénierie des Systèmes Biologiques et des Procédés, Université de Toulouse, INSA, UPS, INP, 135 Avenue de Rangueil, F-31077 Toulouse, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Emond
authors.forename: Cédric
authors.initials: C
authors.lastname: Montanier
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Nicaud
authors.forename: Alain
authors.initials: A
authors.lastname: Marty
authors.forename: Pierre
authors.initials: P
authors.lastname: Monsan
authors.forename: Isabelle
authors.initials: I
authors.lastname: André
authors.forename: Magali
authors.initials: M
authors.lastname: Remaud-Siméon
mesh_terms: D005656:Fungal Proteins; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D008049:Lipase / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D025062:Yarrowia / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D008049:Lipase; C431349:lipase B, Candida antarctica