Relative efficacy of casein or soya protein combined with palm or safflower-seed oil on hyperuricaemia in rats.
Diets that ameliorate the adverse effects of uric acid (UA) on renal damage deserve attention. The effects of casein or soya protein combined with palm or safflower-seed oil on various serum parameters and renal histology were investigated on hyperuricaemic rats. Male Wistar rats administered with oxonic acid and UA to induce hyperuricaemia were fed with casein or soya protein plus palm- or safflower-seed oil-supplemented diets. Normal rats and hyperuricaemic rats with or without allopurinol treatment (150 mg/l in drinking water) were fed with casein plus maize oil-supplemented diets. After 8 weeks, allopurinol treatment and soya protein plus safflower-seed oil-supplemented diet significantly decreased serum UA in hyperuricaemic rats (one-way ANOVA; P < 0.05). In addition, soya protein and casein attenuated hyperuricaemia-induced decreases in serum albumin and insulin, respectively (two-way ANOVA; P < 0.05). Safflower-seed oil significantly decreased serum TAG and UA, whereas palm oil significantly increased serum cholesterol, TAG, blood urea N and creatinine. However, soya protein significantly decreased renal NO and nitrotyrosine and palm oil significantly decreased renal nitrotyrosine, TNF-alpha and interferon-gamma and increased renal transforming growth factor-beta. Casein with safflower-seed oil significantly attenuated renal tubulointerstitial nephritis, crystals and fibrosis. Comparing casein v. soya protein combined with palm or safflower-seed oil, the results support that casein with safflower-seed oil may be effective in attenuating hyperuricaemia-associated renal damage, while soya protein with safflower-seed oil may be beneficial in lowering serum UA and TAG.
Lo
Hui-Chen
HC
Department of Nutritional Science, Fu Jen Catholic University, no. 510 Jhongjheng Road, Sinjhuang City, Taipei County 24205, Taiwan.
Wang
Yao-Horng
YH
Chiou
Hue-Ying
HY
Lai
Shan-Hu
SH
Yang
Yu
Y
D000418:Albumins / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001806:Blood Urea Nitrogen; D002364:Caseins / Q000494:pharmacology / Q000627:therapeutic use*; D002784:Cholesterol / Q000097:blood; D003404:Creatinine / Q000097:blood; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage; D004044:Dietary Proteins / Q000008:administration & dosage; D019587:Dietary Supplements; D004359:Drug Therapy, Combination; D005355:Fibrosis; D033461:Hyperuricemia / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007669:Kidney Calculi / Q000188:drug therapy; D008297:Male; D009395:Nephritis, Interstitial / Q000188:drug therapy; D009569:Nitric Oxide / Q000378:metabolism; D010094:Oxonic Acid; D000073878:Palm Oil; D010938:Plant Oils / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012450:Safflower Oil / Q000494:pharmacology*; D030262:Soybean Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D013025:Glycine max / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D014527:Uric Acid / Q000097:blood*
D000418:Albumins; D002364:Caseins; D004041:Dietary Fats; D004044:Dietary Proteins; D007328:Insulin; D010938:Plant Oils; D030262:Soybean Proteins; D016212:Transforming Growth Factor beta; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D014527:Uric Acid; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; D000073878:Palm Oil; D010094:Oxonic Acid; D012450:Safflower Oil; D007371:Interferon-gamma; D002784:Cholesterol; D003404:Creatinine