title: Expression of fibroblast growth factor receptors 1-4 in human chronic tympanic membrane perforation.
abstract: OBJECTIVE
  To locate fibroblast growth factor receptor (FGFRs) 1-4 in human chronic tympanic membrane (TM) perforation.
  
  
  METHODS
  A sample of human chronic TM perforation was harvested during myringoplasty. The sample was immediately fixed in 4% paraformaldehyde and embedded in OCT compound. Immunohistochemistry was performed with FGFR 1-4 polyclonal antibodies.
  
  
  RESULTS
  FGFRs 1-4 were strongly and weakly expressed in the epidermal and mucosal layer of the TM perforation, respectively.
  
  
  CONCLUSIONS
  As it is impossible to perform quantitative analysis based on the fluorescence intensity of each immunoreactivity, the presence of FGFRs 1-4 in the human chronic TM perforation is shown. The expressions of FGFRs 1-4 indicated that the clinical use of bFGF agent is useful for myringoplasty.
authors.affiliation: Department of Otolaryngology, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan. kakigi-tky@umin.ac.jp
authors.forename: Akinobu
authors.initials: A
authors.lastname: Kakigi
authors.forename: Aya
authors.initials: A
authors.lastname: Uchida
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nishimura
authors.forename: Taizo
authors.initials: T
authors.lastname: Takeda
authors.forename: Setsuko
authors.initials: S
authors.lastname: Takeda
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nakatani
mesh_terms: D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D009225:Myringoplasty; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000378:metabolism*; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000378:metabolism*; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000378:metabolism*; D051499:Receptor, Fibroblast Growth Factor, Type 4 / Q000378:metabolism*; D018058:Tympanic Membrane Perforation / Q000378:metabolism* / Q000601:surgery
chemical_list: D051496:Receptor, Fibroblast Growth Factor, Type 1; D051497:Receptor, Fibroblast Growth Factor, Type 2; D051498:Receptor, Fibroblast Growth Factor, Type 3; D051499:Receptor, Fibroblast Growth Factor, Type 4