Comparison of endothelium-related responses to nucleotides of dog and monkey cerebral arteries.
We compared mechanical responses to uridine-5'triphosphate (UTP) and 2-(methylthio)adenosine-5'diphosphate (2MeSADP) of cerebral arteries isolated from dogs and monkeys. In the dog, UTP induced endothelium-independent contraction, whereas 2MeSADP induced endothelium-dependent relaxation that was abolished by N(G)-nitro-L-arginine (L-NA). In the monkey, both UTP and 2MeSADP induced endothelium-dependent relaxation. L-NA largely inhibited the UTP-induced relaxation whereas it partially inhibited the 2MeSADP-induced relaxation, and both remaining relaxations were abolished by charybdotoxin plus apamin. In conclusion, dog and monkey cerebral arteries respond differentially to UTP and similarly to 2MeSADP; however, involvement of endothelium-derived relaxing factor in the endothelium-dependent relaxation by 2MeSADP is quite different between the two species.
Geddawy
Ayman
A
Department of Pharmacology, Shiga University of Medical Sciences, Otsu, Shiga, Japan.
Shimosato
Takashi
T
Tawa
Masashi
M
Imamura
Takeshi
T
Okamura
Tomio
T
D000818:Animals; D002536:Cerebral Arteries / Q000187:drug effects* / Q000502:physiology; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000502:physiology; D005260:Female; D000882:Haplorhini; D008251:Macaca; D008297:Male; D009711:Nucleotides / Q000494:pharmacology*; D013045:Species Specificity
D009711:Nucleotides