Determination of linezolid in human breast milk by high-performance liquid chromatography with ultraviolet detection.
A rapid and simple HPLC method was developed for the determination of linezolid (LNZ) in human breast milk after a simple protein precipitation with methanol. The chromatographic separation was achieved on a C18 column (5 microm, 250 x 4.6 mm id) using a mobile phase of acetonitrile-10 mM acetic acid (25:75, v/v) at a flow rate of 1 mL/min. The LNZ peak was measured by photodiode array detection at 250 nm. The calibration graph was linear over the range of 0.5-20.0 microg/mL. The limits of detection and quantitation were found to be 0.1 and 0.5 microg/mL, respectively. The precision of the assay and the recovery of LNZ from breast milk at three different concentrations were assessed. The intraday and interday RSD values were found to be < 5%. The mean absolute recovery was 85.33%. The developed method was successfully applied to the determination of LNZ in breast milk obtained from the breastfeeding mother after oral administration of LNZ.
Sagirli
Olcay
O
Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry, 34116 Istanbul, Turkey.
Onal
Armağan
A
Toker
Sidika
S
Oztunç
Aysel
A
D000081:Acetamides / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005615:Freezing; D006801:Humans; D007202:Indicators and Reagents; D000069349:Linezolid; D008895:Milk, Human / Q000737:chemistry*; D023303:Oxazolidinones / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D000081:Acetamides; D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D023303:Oxazolidinones; D012996:Solutions; D000069349:Linezolid