title: Two laccase isoenzymes and a peroxidase of a commercial laccase-producing basidiomycete, Trametes sp. Ha1.
abstract: Three extracellular ligninolytic oxidoreductases that are produced by a commercial laccase-producing Trametes sp. Ha1 were purified and characterized. This fungus showed strong ligninolytic oxidoreductase activity with and without hydrogen peroxide present in the reaction mixture. The oxidoreductase activity was found to be derived from two laccases and a peroxidase. One of the two laccases represents a main component of the commercial laccase preparation from Trametes sp. Ha1. This enzyme had a high thermostability, which makes it attractive for practical applications. The second laccase was induced by the addition of p-xylidine into the culture medium and showed unique characteristics with respect to pI value and substrate specificity. The peroxidase showed wide oxidation activity against aromatic compounds.
authors.affiliation: Daiwa Kasei K.K., 4-19, Hie-cho, Konan, Shiga 520-3203, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Nakatani
authors.forename: Makoto
authors.initials: M
authors.lastname: Hibi
authors.forename: Masashi
authors.initials: M
authors.lastname: Minoda
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
authors.forename: Kenzo
authors.initials: K
authors.lastname: Yokozeki
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D003470:Culture Media / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D005110:Extracellular Space / Q000187:drug effects / Q000201:enzymology; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D042845:Laccase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D013379:Substrate Specificity / Q000187:drug effects; D055454:Trametes / Q000187:drug effects / Q000201:enzymology*; D014992:Xylenes / Q000494:pharmacology
chemical_list: D003470:Culture Media; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D014992:Xylenes; D042845:Laccase; D010544:Peroxidases