Oligosaccharide-induced whey-specific CD25(+) regulatory T-cells are involved in the suppression of cow milk allergy in mice.
Dietary intervention with a unique prebiotic nondigestible carbohydrate mixture has been shown to reduce the development of allergic disease in infants at risk. In this study, the involvement of CD25(+) regulatory T-cells (Treg) in the carbohydrate-induced effects was investigated in mice orally sensitized with whey using adoptive transfer experiments. Donor mice were sensitized with whey and fed a diet containing short-chain galacto-, long-chain fructo- and acidic-oligosaccharides, or a control diet starting 2 wk before sensitization. The acute allergic skin reaction upon intradermal whey challenge was determined and whey-specific Ig were measured. Splenocytes of the donor mice were transferred to naïve recipient mice after partial ex vivo depletion of CD25(+) Treg. The prebiotic diet clearly diminished the acute allergic skin reaction (P < 0.001). Whey-sensitized recipient mice transferred with splenocytes from whey-sensitized, prebiotic-fed donor mice displayed almost complete prevention of the acute allergic skin reaction compared with mice receiving cells from sham-sensitized, prebiotic-fed donor mice (P < 0.001). Partial depletion of CD25(+) T-cells inhibited these effects (P < 0.001), although IgE sensitization was not prevented. This study indicates the involvement of whey-specific CD25(+) Treg in the suppression of the allergic effector response induced by dietary intervention with prebiotics.
Schouten
Bastiaan
B
Department of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
van Esch
Betty C A M
BC
Hofman
Gerard A
GA
Boon
Louis
L
Knippels
Léon M J
LM
Willemsen
Linette E M
LE
Garssen
Johan
J
D019264:Adoptive Transfer; D000818:Animals; D000918:Antibody Specificity; D017449:Dermatitis, Allergic Contact / Q000276:immunology / Q000517:prevention & control; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008892:Milk; D016269:Milk Hypersensitivity / Q000517:prevention & control*; D008894:Milk Proteins / Q000276:immunology*; D009844:Oligosaccharides / Q000494:pharmacology*; D056692:Prebiotics / Q000592:standards; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*; D000067816:Whey Proteins
C508018:Il2ra protein, mouse; D007074:Immunoglobulin G; D053645:Interleukin-2 Receptor alpha Subunit; D008894:Milk Proteins; D009844:Oligosaccharides; D056692:Prebiotics; D000067816:Whey Proteins; D007073:Immunoglobulin E