title: Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression in a variety of organisms, including insects, vertebrates, and plants. miRNAs play important roles in cell development and differentiation as well as in the cellular response to stress and infection. To date, there are limited reports of miRNA identification in mosquitoes, insects that act as essential vectors for the transmission of many human pathogens, including flaviviruses. West Nile virus (WNV) and dengue virus, members of the Flaviviridae family, are primarily transmitted by Aedes and Culex mosquitoes. Using high-throughput deep sequencing, we examined the miRNA repertoire in Ae. albopictus cells and Cx. quinquefasciatus mosquitoes.
  
  
  RESULTS
  We identified a total of 65 miRNAs in the Ae. albopictus C7/10 cell line and 77 miRNAs in Cx. quinquefasciatus mosquitoes, the majority of which are conserved in other insects such as Drosophila melanogaster and Anopheles gambiae. The most highly expressed miRNA in both mosquito species was miR-184, a miRNA conserved from insects to vertebrates. Several previously reported Anopheles miRNAs, including miR-1890 and miR-1891, were also found in Culex and Aedes, and appear to be restricted to mosquitoes. We identified seven novel miRNAs, arising from nine different precursors, in C7/10 cells and Cx. quinquefasciatus mosquitoes, two of which have predicted orthologs in An. gambiae. Several of these novel miRNAs reside within a ~350 nt long cluster present in both Aedes and Culex. miRNA expression was confirmed by primer extension analysis. To determine whether flavivirus infection affects miRNA expression, we infected female Culex mosquitoes with WNV. Two miRNAs, miR-92 and miR-989, showed significant changes in expression levels following WNV infection.
  
  
  CONCLUSIONS
  Aedes and Culex mosquitoes are important flavivirus vectors. Recent advances in both mosquito genomics and high-throughput sequencing technologies enabled us to interrogate the miRNA profile in these two species. Here, we provide evidence for over 60 conserved and seven novel mosquito miRNAs, expanding upon our current understanding of insect miRNAs. Undoubtedly, some of the miRNAs identified will have roles not only in mosquito development, but also in mediating viral infection in the mosquito host.
authors.affiliation: Department of Molecular Genetics and Microbiology and Center for Virology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Skalsky
authors.forename: Dana L
authors.initials: DL
authors.lastname: Vanlandingham
authors.forename: Frank
authors.initials: F
authors.lastname: Scholle
authors.forename: Stephen
authors.initials: S
authors.lastname: Higgs
authors.forename: Bryan R
authors.initials: BR
authors.lastname: Cullen
mesh_terms: D000330:Aedes / Q000235:genetics* / Q000821:virology; D000818:Animals; D002460:Cell Line; D019295:Computational Biology; D003465:Culex / Q000235:genetics* / Q000821:virology; D005260:Female; D007303:Insect Vectors / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D017423:Sequence Analysis, RNA; D014902:West Nile virus
chemical_list: D035683:MicroRNAs