Reductive degradation of pyrazine-2-carboxylate by a newly isolated Stenotrophomonas sp. HCU1.
A bacterium growing on pyrazine-2-carboxylate broth was isolated, purified and identified as a strain of Stenotrophomonas sp. based on polyphasic taxonomic analyses and designated as strain HCU1. 16S rRNA gene sequence of strain HCU1 showed 98.7% sequence similarity with the type strain of Stenotrophomonas maltophilia belonging to Gammaproteobacteria. Growth of strain HCU1 was demonstrated when pyrazine-2-carboxylate was used as a sole source of nitrogen. Ring reduction of pyrazine-2-carboxylate was shown as increase in absorbance at 268 nm and the reduced product was confirmed as 1,2,5,6-tetrahydropyrazine-2-carboxylate, while a ring opened product, 2-amino-2-hydroxy-3-(methylamino) propanoic acid (with a loss in carbon atom), indicated a reductive degradation of pyrazine-2-carboxylate by strain HCU1.
Rajini
K S
KS
Department of Plant Sciences, University of Hyderabad, Hyderabad, India. keerthirajini5@yahoo.co.in
Sasikala
Ch
Ch
Ramana
Ch V
ChV
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D057075:Enzyme Assays; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry; D010802:Phylogeny; D011719:Pyrazines / Q000737:chemistry / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D020588:Stenotrophomonas / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*
D001426:Bacterial Proteins; D010455:Peptides; D011719:Pyrazines; D012336:RNA, Ribosomal, 16S; C553563:pyrazine-2-carboxylate