title: Determination of aflatoxin B(1) in alcoholic beverages: comparison of one- and two-photon-induced fluorescence.
abstract: The qualitative and quantitative analysis of aflatoxin B(1) in a model system (water/ethanol), in different wines and in beer using one- and two-photon-induced fluorescence is discussed. The absorption and fluorescence properties of aflatoxin B(1) depend on the solvent and pH. The two-photon-absorption cross-section was calculated for aflatoxin B(1) in beer and wine (sigma (2) approximately 25 GM) for excitation at 720 nm. A comparison of the one- and two-photon- induced fluorescence results showed that the disturbance due to background emission originating from matrix constituents is significantly reduced under two-photon-excitation conditions. The limit of detection for the one- and two-photon-induced fluorescence was determined.
authors.affiliation: Department of Chemistry (Physical Chemistry), University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam-Golm, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Rasch
authors.affiliation: Department of Chemistry (Physical Chemistry), University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam-Golm, Germany.
authors.forename: Maike
authors.initials: M
authors.lastname: Böttcher
authors.affiliation: Department of Chemistry (Physical Chemistry), University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam-Golm, Germany. kumke@uni-potsdam.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Kumke
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D000434:Alcoholic Beverages / Q000032:analysis*; D005453:Fluorescence; D057230:Limit of Detection; D017785:Photons; D013050:Spectrometry, Fluorescence
chemical_list: D016604:Aflatoxin B1