Screening of ovarian steroidogenic pathway in Ciona intestinalis and its modulation after tributyltin exposure.
In this study, we have identified several ovarian steroids in Ciona with high similarity to vertebrate steroids and showed that cholesterol, corticosterone, dehydroepiandrosterone, estrone, estradiol-17beta, testosterone, pregnenolone, progesterone, have identical molecular spectra with vertebrate steroids. In addition, we have studied the effects of an endocrine disruptor (tributyltin: TBT) on these sex hormones and their precursors, ovarian morphology, and gene expression of some key enzymes in steroidogenic pathway in the ovary of Ciona. Ovarian specimens were cultured in vitro using different concentrations of TBT (10(-5), 10(-4) and 10(-3)M). Ethanol was used as solvent control. Gene expression analysis was performed for adrenodoxin (ADREN) and adrenodoxin reductase (ADOX) (mediators of acute steroidogenesis) and 17beta-hydroxysteroid dehydrogenase (17beta-HSD). These transcripts were detected and measured by quantitative (real-time) polymerase chain reaction (qPCR). Sex steroids and their precursors were identified and quantified by a gas chromatography-mass spectroscopy (GC-MS) method. Exposure of Ciona ovaries to TBT produced modulations (either increased or decreased) of sterols and sex steroid levels, whereas no significant differences in ADREN, ADOX or 17beta-HSD mRNA expression patterns were observed. Histological analysis shows that TBT produced several modifications on Ciona ovarian morphology that includes irregular outline of nuclear membrane, less compacted cytoplasm, in addition to test and granulosa cells that were detached from the oocyte membrane. Given that the ascidians represent very simple experimental models for the study of endocrine disruption by environmental contaminants, our findings provide excellent models for multiple identification and quantification of sex steroid and their precursors in biological samples exposed to endocrine-disrupting chemicals and for direct extrapolation of such effects across taxonomic groups and phyla. In addition, these results suggest that Cionaintestinalis may be a suitable species for molecular ecotoxicological studies and biomarker model for endocrine-disrupting effects in marine invertebrates.
Cangialosi
Maria Vittoria
MV
Department of Animal Biology G. Reverberi, University of Palermo, Italy.
Puccia
Egidio
E
Mazzola
Antonio
A
Mansueto
Valentina
V
Arukwe
Augustine
A
D015067:17-Hydroxysteroid Dehydrogenases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000325:Adrenodoxin / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002784:Cholesterol / Q000032:analysis; D002938:Ciona intestinalis / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D003345:Corticosterone / Q000032:analysis; D003687:Dehydroepiandrosterone / Q000032:analysis; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000032:analysis; D004970:Estrone / Q000032:analysis; D005260:Female; D005287:Ferredoxin-NADP Reductase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D015870:Gene Expression / Q000187:drug effects; D012739:Gonadal Steroid Hormones / Q000235:genetics / Q000378:metabolism*; D010053:Ovary / Q000033:anatomy & histology / Q000187:drug effects* / Q000502:physiology; D011284:Pregnenolone / Q000032:analysis; D011374:Progesterone / Q000032:analysis; D013739:Testosterone / Q000032:analysis; D014220:Trialkyltin Compounds / Q000633:toxicity*
D052244:Endocrine Disruptors; D012739:Gonadal Steroid Hormones; D014220:Trialkyltin Compounds; D000325:Adrenodoxin; D004970:Estrone; D013739:Testosterone; D003687:Dehydroepiandrosterone; D011374:Progesterone; D004958:Estradiol; C011559:tributyltin; D011284:Pregnenolone; D002784:Cholesterol; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; D005287:Ferredoxin-NADP Reductase; D003345:Corticosterone