title: Role of decay-accelerating factor in regulating survival of human cervical cancer cells.
abstract: BACKGROUND
  Decay-accelerating factor (DAF) is one of the key molecules involved in cell protection against autologous complement, which restricts the action of complement at critical stages of the cascade reaction. The effect of DAF on the survival of human cervical cancer cell (ME180) has not been demonstrated.
  
  
  METHODS
  In this study we applied, for the first time, small interference RNA (siRNA) to knock down the expression of the DAF with the aim of exploiting complement more effectively for tumor cell damage. Meanwhile, we investigated the effects of DAF on the viability and migration, moreover the proliferation of ME180 cell.
  
  
  RESULTS
  The results showed that the expression of DAF was significantly increased in human cervical cancer tissues. SiRNA inhibition of DAF expression enhanced complement-dependent cytolysis up to 32% in ME180 cells, which contributed to the control of C3 activation and increased the cells viability, migration and augment the number of ME180 cells.
  
  
  CONCLUSIONS
  These data indicated that DAF siRNA described in this study may offer an additional alternative to improve the efficacy of antibody- and complement-based cancer immunotherapy.
authors.affiliation: Clinical Laboratory, Nanjing Maternity and Child Health Care Hospital, Tianfei Alley, Nanjing Mochou Road, Nanjing 210004, People's Republic of China.
authors.forename: Ling-Juan
authors.initials: LJ
authors.lastname: Gao
authors.forename: Lan
authors.initials: L
authors.lastname: Ding
authors.forename: Shu-Yu
authors.initials: SY
authors.lastname: Guo
authors.forename: You-Qun
authors.initials: YQ
authors.lastname: Cai
authors.forename: Ya-Juan
authors.initials: YJ
authors.lastname: Su
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Ping-Qing
authors.initials: PQ
authors.lastname: Gu
mesh_terms: D018958:CD55 Antigens / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D050577:Complement C3-C5 Convertases / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006801:Humans; D034741:RNA, Small Interfering / Q000378:metabolism; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D018958:CD55 Antigens; D034741:RNA, Small Interfering; D050577:Complement C3-C5 Convertases