Photothermal optical coherence tomography in ex vivo human breast tissues using gold nanoshells.
We demonstrate photothermal optical coherence tomography (OCT) imaging in highly scattering human breast tissue ex vivo. A 120 kHz axial scan rate, swept-source phase-sensitive OCT system at 1300 nm was used to detect phase changes induced by 830 nm photothermal excitation of gold nanoshells. Localized phase modulation was observed 300-600 microm deep in scattering tissue using an excitation power of only 22 mW at modulation frequencies up to 20 kHz. This technique enables integrated structural and molecular-targeted imaging for cancer markers using nanoshells.
Zhou
Chao
C
Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Tsai
Tsung-Han
TH
Adler
Desmond C
DC
Lee
Hsiang-Chieh
HC
Cohen
David W
DW
Mondelblatt
Amy
A
Wang
Yihong
Y
Connolly
James L
JL
Fujimoto
James G
JG
D001940:Breast / Q000166:cytology*; D003287:Contrast Media / Q000737:chemistry*; D005260:Female; D006046:Gold / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D015336:Molecular Probe Techniques; D053758:Nanoparticles / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000379:methods*
D003287:Contrast Media; D006046:Gold