Ameloblast differentiation in the human developing tooth: effects of extracellular matrices.
Tooth enamel is formed by epithelially-derived cells called ameloblasts, while the pulp dentin complex is formed by the dental mesenchyme. These tissues differentiate with reciprocal signaling interactions to form a mature tooth. In this study we have characterized ameloblast differentiation in human developing incisors, and have further investigated the role of extracellular matrix proteins on ameloblast differentiation. Histological and immunohistochemical analyses showed that in the human tooth, the basement membrane separating the early developing dental epithelium and mesenchyme was lost shortly before dentin deposition was initiated, prior to enamel matrix secretion. Presecretary ameloblasts elongated as they came into contact with the dentin matrix, and then shortened to become secretory ameloblasts. In situ hybridization showed that the presecretory stage of odontoblasts started to express type I collagen mRNA, and also briefly expressed amelogenin mRNA. This was followed by upregulation of amelogenin mRNA expression in secretory ameloblasts. In vitro, amelogenin expression was upregulated in ameloblast lineage cells cultured in Matrigel, and was further up-regulated when these cells/Matrigel were co-cultured with dental pulp cells. Co-culture also up-regulated type I collagen expression by the dental pulp cells. Type I collagen coated culture dishes promoted a more elongated ameloblast lineage cell morphology and enhanced cell adhesion via integrin alpha2beta1. Taken together, these results suggest that the basement membrane proteins and signals from underlying mesenchymal cells coordinate to initiate differentiation of preameloblasts and regulate type I collagen expression by odontoblasts. Type I collagen in the dentin matrix then anchors the presecretary ameloblasts as they further differentiate to secretory cells. These studies show the critical roles of the extracellular matrix proteins in ameloblast differentiation.
He
Pingping
P
Department of Orofacial Sciences, University of California-San Francisco, 521 Parnassus Avenue, San Francisco, CA 94143-0640, USA.
Zhang
Yan
Y
Kim
Seong Oh
SO
Radlanski
Ralf J
RJ
Butcher
Kristin
K
Schneider
Richard A
RA
DenBesten
Pamela K
PK
D000565:Ameloblasts / Q000502:physiology; D053523:Amelogenin / Q000235:genetics / Q000502:physiology*; D001485:Basement Membrane / Q000502:physiology* / Q000648:ultrastructure; D015153:Blotting, Western; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D024042:Collagen Type I / Q000235:genetics / Q000502:physiology*; D005333:Fetus; D006651:Histocytochemistry; D006801:Humans; D017403:In Situ Hybridization; D007180:Incisor / Q000502:physiology* / Q000648:ultrastructure; D008858:Microscopy, Phase-Contrast; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology*
D053523:Amelogenin; D024042:Collagen Type I; D012333:RNA, Messenger