[Using IRAP markers for analysis of genetic variability in populations of resource and rare species of plants].
Species-specific LTR retrotransposons were first cloned in five rare relic species of drug plants located in the Perm' region. Sequences of LTR retrotransposons were used for PCR analysis based on amplification of repeated sequences from LTR or other sites of retrotransposons (IRAP). Genetic diversity was studied in six populations of rare relic species of plants Adonis vernalis L. by means of the IRAP method; 125 polymorphic IRAP-markers were analyzed. Parameters for DNA polymorphism and genetic diversity of A. vernalis populations were determined.
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D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D017343:Genes, Plant; D005819:Genetic Markers; D014644:Genetic Variation; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D018626:Retroelements / Q000235:genetics*; D013045:Species Specificity; D020079:Terminal Repeat Sequences / Q000235:genetics
D017931:DNA Primers; D005819:Genetic Markers; D018626:Retroelements