Liquid chromatography/mass spectrometry-based structural analysis of soyasaponin Ab metabolites by human fecal microflora.
Soyasaponin Ab, a major constituent of soybean by human intestinal microflora, was anaerobically incubated with fecal suspensions from ten individuals for 48 h and its metabolites were measured by LC-MS/MS analysis. Ten metabolites were detected. The spectra of the parental constituent soyasaponin Ab showed a peak at m/z 1435 [M-H](-) ion and those of its nine metabolites showed peaks at m/z 1310.0 [M-3C(2)H(2)O-H](-) ion, m/z 1267.9 [M-4C(2)H(2)O-H](-) ion, m/z 1105.3 [M-Glc-4C(2)H(2)O-H](-) ion, m/z 973.2 [M-Glc-Ara-4C(2)H(2)O-H](-) ion, m/z 943.4 [M-2Glc-4C(2)H(2)O-H](-) ion, m/z 811.1 [M-2Glc-Ara-4C(2)H(2)O-H](-) ion, m/z 781.2 [M-2Glc-Gal-4C(2)H(2)O-H](-) ion, m/z 649.0 [M-2Glc-Gal-Ara-4C(2)H(2)O-H](-) ion, and m/z 458.8 [Soyasapogenol A+H](+) ion. Metabolic activity varied significantly between individuals. The metabolic pathway was classified into two groups: the first group potently produced soyasapogenol A and the second group accumulated soyasapogenol A 3-beta-D-glucuronide.
Chang
Seo-Young
SY
Department of Food and Nutrition, Kyung Hee University, Seoul 130-701, Republic of Korea.
Han
Myung Joo
MJ
Joh
Eun-Ha
EH
Kim
Dong-Hyun
DH
D000328:Adult; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002853:Chromatography, Liquid / Q000379:methods*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008297:Male; D008969:Molecular Sequence Data; D012503:Saponins / Q000737:chemistry* / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*
D012503:Saponins; C551307:soyasaponin Ab