Embryonic MGE precursor cells grafted into adult rat striatum integrate and ameliorate motor symptoms in 6-OHDA-lesioned rats.
We investigated a strategy to ameliorate the motor symptoms of rats that received 6-hydroxydopamine (6-OHDA) lesions, a rodent model of Parkinson's disease, through transplantation of embryonic medial ganglionic eminence (MGE) cells into the striatum. During brain development, embryonic MGE cells migrate into the striatum and neocortex where they mature into GABAergic interneurons and play a key role in establishing the balance between excitation and inhibition. Unlike most other embryonic neurons, MGE cells retain the capacity for migration and integration when transplanted into the postnatal and adult brain. We performed MGE cell transplantation into the basal ganglia of control and 6-OHDA-lesioned rats. Transplanted MGE cells survived, differentiated into GABA(+) neurons, integrated into host circuitry, and modified motor behavior in both lesioned and control rats. Our data suggest that MGE cell transplantation into the striatum is a promising approach to investigate the potential benefits of remodeling basal ganglia circuitry in neurodegenerative diseases.
Martínez-Cerdeño
Verónica
V
Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research at UCSF, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA. vmartinezcerdeno@ucdavis.edu
Noctor
Stephen C
SC
Espinosa
Ana
A
Ariza
Jeanelle
J
Parker
Philip
P
Orasji
Samantha
S
Daadi
Marcel M
MM
Bankiewicz
Krystof
K
Alvarez-Buylla
Arturo
A
Kriegstein
Arnold R
AR
D000375:Aging; D000818:Animals; D001522:Behavior, Animal; D002454:Cell Differentiation; D002465:Cell Movement; D002470:Cell Survival; D017690:Cell Transplantation; D003342:Corpus Striatum / Q000166:cytology* / Q000378:metabolism* / Q000601:surgery; D005260:Female; D008473:Median Eminence / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D009043:Motor Activity; D016627:Oxidopamine / Q000378:metabolism*; D051381:Rats; D017447:Receptors, Dopamine D1 / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D005680:gamma-Aminobutyric Acid; D016627:Oxidopamine