title: Shiga toxin 2-specific but not shiga toxin 1-specific human monoclonal antibody protects piglets challenged with enterohemorrhagic Escherichia coli producing shiga toxin 1 and shiga toxin 2.
abstract: Escherichia coli strains that produce Shiga toxin 2 (Stx2) are isolated from hemolytic-uremic syndrome (HUS) cases more frequently than are strains that produce both Shiga toxin 1 (Stx1) and Stx2, whereas strains that produce only Stx1 are rarely isolated from HUS cases. Studies have implicated Stx2 as the sole contributor to acute kidney failure and other systemic complications in humans. The aim of the present study was to determine whether Stx2-specific antibody would be as effective against Shiga toxin-producing Escherichia coli (STEC) strains that produce both Stx1 and Stx2 as it is against strains that produce only Stx2, compared with Stx1-specific antibody. We found that Stx2-specific and Stx1-specific antibodies protected 100% and 0% of piglets, respectively, against oral challenge with a Stx1- and Stx2-producing STEC strain. We conclude that Stx2-specific antibody is sufficient to protect piglets, and possibly humans, against STEC strains that produce both toxins.
authors.affiliation: Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA.
authors.forename: Kwang-Il
authors.initials: KI
authors.lastname: Jeong
authors.forename: Saul
authors.initials: S
authors.lastname: Tzipori
authors.forename: Abhineet S
authors.initials: AS
authors.lastname: Sheoran
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000918:Antibody Specificity; D054324:Enterohemorrhagic Escherichia coli / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D006801:Humans; D022622:Shiga Toxin 1 / Q000096:biosynthesis / Q000276:immunology*; D022641:Shiga Toxin 2 / Q000096:biosynthesis / Q000276:immunology*; D013552:Swine
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2