Production of a novel extracellular acidic lipase from Pseudomonas gessardii using slaughterhouse waste as a substrate.
An isolate exhibiting high extracellular lipolytic activity was identified as Pseudomonas gessardii by 16S rDNA gene sequence analysis. The slaughterhouse waste, goat tallow, was used as a lipid substrate for the production of acidic lipase by P. gessardii. The maximum lipase activity of 156 U/ml was observed at an acidic pH of 3.5 and at 0.31 g substrate concentration. The purification steps resulted in the isolation of acidic lipase with a specific activity of 1,473 U/mg and a molecular weight of 94 kDa. One interesting feature of this purified lipase is its stability at highly acidic pH ranging from 2.0 to 5.5 with a high molecular weight. The amino acid composition was determined using HPLC. This acidic lipase has potential applications in the medicinal field as a substitute for pancreatic lipases for enzyme therapy, oleochemical and in biotechnological industries.
Ramani
K
K
Environmental Technology Division, Central Leather Research Institute, Council of Scientific and Industrial Research, Adyar, Chennai, Tamilnadu, India.
Chockalingam
Evvie
E
Sekaran
G
G
D000003:Abattoirs; D000818:Animals; D004795:Enzyme Stability; D005223:Fats / Q000378:metabolism; D006041:Goats; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D007700:Kinetics; D008049:Lipase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D008066:Lipolysis; D008970:Molecular Weight; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012988:Soil Microbiology
D005223:Fats; C013698:tallow; D008049:Lipase