title: [Effects of silencing transforming growth factor-beta1 by RNAi upon transdifferentiation of renal allograft tubular epithelial cells in rats].
abstract: OBJECTIVE
  To investigate the effects of shRNA-transforming growth factor (TGF)-beta1 plasmid upon epithelial-myofibroblast transdifferentiation of renal allograft in rats.
  
  
  METHODS
  Divided the Wistar rats into 4 groups: Group J (sham-operated group), T (plasmid group), H (vacant plasmid group) and Y (simply transplantation group). The SD to Wistar rat transplant kidney-sclerosis accelerated model was constructed and transfected with the plasmid based on hydromechanics. Transplanted kidneys were collected at Months 1, 2 and 3 post-transplantation. The gene transcriptional levels of TGF-beta1 and E-cadherin were detected by RT-PCR and the protein variation of E-cadherin was examined by Western blotting. The pathological changes and infiltrated inflammatory cells were assessed by HE staining and the immunohistochemical staining of E-cadherin and alpha-SMA used to label epithelial cells and fibroblast in order to exhibit cell transdifferentiation.
  
  
  RESULTS
  Compared with Group H and Y, the mRNA transcription of TGF-beta1 was obviously inhibited in the Group T: at Month 3, the TGF-beta1 mRNA of Group T is 0.73 +/- 0.08, significantly lower than Group H and Y (0.92 +/- 0.07 and 0.95 +/- 0.04, both P < 0.01); the expression of E-cadherin was maintained at a high level: at the Month 3, the E-cadherin mRNA of Group T is 0.39 +/- 0.11, significantly higher than Group H and Y (0.15 +/- 0.07, and 0.17 +/- 0.06, both P < 0.01); the E-cadherin protein of Group T is 0.38 +/- 0.08, significantly higher than group H and Y (0.15 +/- 0.07 and 0.15 +/- 0.07, both P < 0.01); epithelial cells were much more and fibroblast was much less than that of Group H and Y; there were also less infiltrated chronic inflammatory cells and extracellular matrix deposition in the Group T.
  
  
  CONCLUSIONS
  The shRNA-TGF-beta1 plasmid can inhibit the epithelial-myofibroblast transdifferentiation of renal allograft in rats. The mechanisms may be associated with its effects of down-regulating TGF-beta1 and up-regulating E-cadherin.
authors.affiliation: Department of Urology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yu-guo
authors.initials: YG
authors.lastname: Xia
authors.forename: Xiao-hou
authors.initials: XH
authors.lastname: Wu
authors.forename: Zhi-kang
authors.initials: ZK
authors.lastname: Yin
authors.forename: Chun-li
authors.initials: CL
authors.lastname: Luo
authors.forename: Jia-mo
authors.initials: JM
authors.lastname: Zhang
authors.forename: Hong-lin
authors.initials: HL
authors.lastname: Cheng
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D054338:Cell Transdifferentiation; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D005347:Fibroblasts; D007668:Kidney / Q000473:pathology*; D016030:Kidney Transplantation; D007684:Kidney Tubules / Q000473:pathology; D010957:Plasmids; D034622:RNA Interference; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000494:pharmacology*
chemical_list: D015820:Cadherins; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1