title: Electrochemical DNA sandwich assay with a lipase label for attomole detection of DNA.
abstract: A fast and sensitive electrochemical lipase-based sandwich hybridization assay for detection of attomole levels of DNA has been developed. A combination of magnetic beads, used for pre-concentration and bioseparation of the analyte with a lipase catalyst label allowed detection of DNA with a limit of 20 amol.
authors.affiliation: Centre for DNA Nanotechnology, Department of Chemistry and iNANO, Aarhus University, Langelandsgade 140, DK-8000 Aarhus C, Denmark. e_ferapontova@chem.au.dk
authors.forename: Elena E
authors.initials: EE
authors.lastname: Ferapontova
authors.forename: Majken N
authors.initials: MN
authors.lastname: Hansen
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Saunders
authors.forename: Stepan
authors.initials: S
authors.lastname: Shipovskov
authors.forename: Duncan S
authors.initials: DS
authors.lastname: Sutherland
authors.forename: Kurt V
authors.initials: KV
authors.lastname: Gothelf
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D008280:Magnetics; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D006046:Gold; D004247:DNA; D008049:Lipase