title: Collagen-embedded hydroxylapatite-beta-tricalcium phosphate-silicon dioxide bone substitute granules assist rapid vascularization and promote cell growth.
abstract: In the present study we assessed the biocompatibility in vitro and in vivo of a low-temperature sol-gel-manufactured SiO(2)-based bone graft substitute. Human primary osteoblasts and the osteoblastic cell line, MG63, cultured on the SiO(2) biomatrix in monoculture retained their osteoblastic morphology and cellular functionality in vitro. The effect of the biomaterial in vivo and its vascularization potential was tested subcutaneously in Wistar rats and demonstrated both rapid vascularization and good integration within the peri-implant tissue. Scaffold degradation was progressive during the first month after implantation, with tartrate-resistant acid phosphatase-positive macrophages being present and promoting scaffold degradation from an early stage. This manuscript describes successful osteoblastic growth promotion in vitro and a promising biomaterial integration and vasculogenesis in vivo for a possible therapeutic application of this biomatrix in future clinical studies.
authors.affiliation: Institute of Pathology, Johannes Gutenberg-University Mainz, Germany. ghanaati@uni-mainz.de
authors.forename: Shahram M
authors.initials: SM
authors.lastname: Ghanaati
authors.forename: Benjamin W
authors.initials: BW
authors.lastname: Thimm
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Unger
authors.forename: Carina
authors.initials: C
authors.lastname: Orth
authors.forename: Thomas
authors.initials: T
authors.lastname: Kohler
authors.forename: Mike
authors.initials: M
authors.lastname: Barbeck
authors.forename: Ralph
authors.initials: R
authors.lastname: Müller
authors.forename: C James
authors.initials: CJ
authors.lastname: Kirkpatrick
mesh_terms: D001808:Blood Vessels / Q000166:cytology* / Q000254:growth & development*; D018786:Bone Substitutes / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D018919:Neovascularization, Physiologic / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: C519914:BONITmatrix; D018786:Bone Substitutes; D002130:Calcium Phosphates; D012822:Silicon Dioxide; D003094:Collagen