title: In vitro selection of RNA aptamers to a protein target by filter immobilization.
abstract: This unit describes the selection of aptamers from a pool of single-stranded RNA by binding to a protein target. Aptamers generated from this selection experiment can potentially act as protein function inhibitors, and may find applications as therapeutic or diagnostic reagents. A pool of dsDNA is used to generate an ssRNA pool, which is mixed with the protein target. Bound complexes are separated from unbound reagents by filtration, and the RNA:protein complexes are amplified by a combination of reverse transcription, PCR, and in vitro transcription.
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas, Austin, Texas, USA.
authors.forename: Bradley
authors.initials: B
authors.lastname: Hall
authors.forename: Seyed
authors.initials: S
authors.lastname: Arshad
authors.forename: Kyunghyun
authors.initials: K
authors.lastname: Seo
authors.forename: Catherine
authors.initials: C
authors.lastname: Bowman
authors.forename: Meredith
authors.initials: M
authors.lastname: Corley
authors.forename: Sulay D
authors.initials: SD
authors.lastname: Jhaveri
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism*; D005374:Filtration / Q000379:methods*; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D007202:Indicators and Reagents; D011506:Proteins