title: Immunostimulatory effects of extract of Pulicaria crispa before and after Schistosoma mansoni infection.
abstract: The immunostimulatory effects of methanolic extract from Pulicaria crispa were investigated in mice before and after infection with Schistosoma mansoni. Mice were subjected for daily intra-peritoneal injection by the extract (33 ng/mouse) for 10 successive days followed by infecting every mouse with 100 S. mansoni cercariae. Treatment with the extract induced significant increase (p < 0.05) in sera-IL-2 before and after infection. Upon using soluble worm antigen preparation or cancer bladder homogenates as antigens in ELISA, the detected levels of IgG were significantly (p < 0.05) higher in sera from treated-infected mice than untreated P. crispa infected mice. Using crude Escherichia coli lysate as an antigen in ELISA, it was detected a significant (p < 0.05) increase in IgG levels in sera from the extract-treated mice before and after infection.
authors.affiliation: Therapeutic Chemistry Department, National Research Center, Dokki, Cairo 12311, Egypt.
authors.forename: Amany S
authors.initials: AS
authors.lastname: Maghraby
authors.forename: Nagwa
authors.initials: N
authors.lastname: Shalaby
authors.forename: Howida I
authors.initials: HI
authors.lastname: Abd-Alla
authors.forename: Samia A
authors.initials: SA
authors.lastname: Ahmed
authors.forename: Hussein M
authors.initials: HM
authors.lastname: Khaled
authors.forename: Mahmoud M
authors.initials: MM
authors.lastname: Bahgat
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007274:Injections, Intraperitoneal; D007376:Interleukin-2 / Q000097:blood; D051379:Mice; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D052587:Pulicaria; D012550:Schistosoma mansoni / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000188:drug therapy* / Q000276:immunology / Q000469:parasitology; D012556:Schistosomicides / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007376:Interleukin-2; D010936:Plant Extracts; D012556:Schistosomicides