title: Inhibition of microbial growth and enrichment of gamma-aminobutyric acid during germination of brown rice by electrolyzed oxidizing water.
abstract: Electrolyzed oxidizing water (EOW) has been regarded as a potential environmentally friendly broad spectrum microbial decontaminant. EOW with a pH of 3.0 and oxidation reduction potential of 1,079.0 mV were generated by the electrolysis of a dilute NaCl solution (20 mM) in an electrochemical cell. The effects of EOW, 1% NaClO solution, and alkaline electrolyzed water on controlling microbial growth, germination ratio, and enrichment of gamma-aminobutyric acid in germinated brown rice (GBR) were evaluated in this study. Results show that EOW was the most effective at inhibiting microbial growth during germination. Rinsing the rice grains with EOW at 12-h intervals resulted in aerobic plate count reductions of 4.82 log CFU/g, while soaking resulted in bacterial count reductions of 5.38 log CFU/g after 72 h of germination. Moreover, EOW significantly enriched gamma-aminobutyric acid content in GBR (P < 0.05); content was increased 1.6 times in grain rinsed with EOW and 1.8 times in grain soaked in EOW. The findings indicate that EOW is a feasible disinfectant for industrial GBR production.
authors.affiliation: Department of Food Sciences & Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: Zhan-Hui
authors.initials: ZH
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Li-Te
authors.initials: LT
authors.lastname: Li
authors.forename: Rempel B
authors.initials: RB
authors.lastname: Curtis
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Kong
authors.forename: R Gary
authors.initials: RG
authors.lastname: Fulcher
authors.forename: Gong
authors.initials: G
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
mesh_terms: D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004572:Electrolysis; D005506:Food Contamination / Q000517:prevention & control*; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D018525:Germination; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012275:Oryza / Q000382:microbiology*; D010084:Oxidation-Reduction; D016896:Treatment Outcome; D014867:Water / Q000737:chemistry / Q000494:pharmacology*; D014871:Water Microbiology; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
chemical_list: D014867:Water; D005680:gamma-Aminobutyric Acid