[Study on callus induction of Ferula sinkiangensis].
OBJECTIVE
To explore the effect of different culture conditions and hormone combinations on callus induction of Ferula sinkiangensis.


METHODS
Using different seedling age of Ferula sinkiangensis young cotyledon, hypocotyl, radicle as explants, which were cultivated on differentiation medium with different hormone combinations, to study the effects on Ferulic callus by different explants and hormone combinations.


RESULTS
Ferulic seedlings with no true leaf was more favorable to the callus, the optimum medium for hypocotyl induction was MS + 2,4-D (1.0 mg/L) + KT (1.5 mg/L), for radicle induction was MS + NAA (0.5 mg/L) + 6-BA (0.5 mg/L), and the best subculture medium was MS + NAA (1.5 mg/L) + 6-BA (2.5 mg/L).


CONCLUSIONS
NAA,2,4-D and 6-BA must have the inductive effects, 6-BA is better than the KT in the process of proliferation and the medium containing GA3 is conducive to the process of subculture embryogenic callus production.
Zhu
Jun
J
Xin Jiang Institute of Chinese Traditional Medical and Ethical Materia Medica, Urumqi 830002, China. zhujunlhao@163.com
Li
Xiao-jin
XJ
Kaisa
Sulaiman
S
Jia
Xiao-guang
XG
D002681:China; D003470:Culture Media / Q000737:chemistry; D005309:Ferula / Q000254:growth & development* / Q000378:metabolism; D006728:Hormones / Q000378:metabolism / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods
D003470:Culture Media; D006728:Hormones; D010937:Plant Growth Regulators