Interaction of Pb2+, PbMe22+ and PbPh22+ with 3-(phenyl)-2-sulfanylpropenoic acid: a coordinative and toxicological approach.
We investigated the reaction of Pb(2+), PbMe(2)(2+) and PbPh(2)(2+) with 3-(phenyl)-2-sulfanylpropenoic acid (H(2)pspa) to give the complexes [Pb(pspa)], [PbMe(2)(pspa)], [PbPh(2)(pspa)], [HQ](2)[Pb(pspa)(2)] and [HQ[(2)[PbPh(2)(pspa)(2)] (HQ=diisopropylammonium), which were characterized by IR and NMR ((1)H, (13)C and (207)Pb) spectroscopy and by fast atom bombardment (FAB) spectrometry. The structures of [PbMe(2)(pspa)], [PbPh(2)(pspa)], [PbPh(2)(pspa)(dmso)].dmso and [HQ[(2)[PbPh(2)(pspa)(2)] are interesting examples of unexplored Pb coordination kernels and supramolecular association. Pig renal proximal tubule LLC-PK1 culture cells were used to determine in vitro the effect of the pretreatment with H(2)pspa (alone or combined with vitamin B(6)) and [HQ](2)[Zn(pspa)(2)] on the cytotoxicity of PbMe(2)(2+) and PbPh(2)(2+) by comparing the results with those of meso-2,3-dimercaptosuccinic acid (dmsa). The results show that the cell viability was scarcely affected by these agents. The ability of these reagents to decorporate lead was investigated in vivo by analysing the lead levels in the liver, kidney, brain and blood. In the case of the dimethyl derivative, and under certain protocols, undesirable effects such as an increase in brain and liver lead levels were detected. These increases were not detected when the diphenyl derivative was assayed but in this case a positive effect was not identified either. The blood lead levels also increased in the case of the dimethyl derivative and the activity of delta-ALAD was significantly recovered upon treatment with vitamin B(6) or H(2)pspa; neither the blood lead levels nor the delta-ALAD activity was modified in the case of the diphenyl derivative.
Félix Camiña
M
M
Departamento de Bioquímica, Facultade de Farmacia, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Galicia, Spain.
Casas
José S
JS
Victoria Castaño
M
M
Couce
María D
MD
Gato
Angeles
A
Herbello-Hermelo
Paloma
P
Sánchez
Agustín
A
Sordo
José
J
Dolores Torres
M
M
D000143:Acids / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D018360:Crystallography, X-Ray; D006801:Humans; D007854:Lead / Q000737:chemistry* / Q000378:metabolism; D007855:Lead Poisoning / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D051381:Rats; D017207:Rats, Sprague-Dawley
D000143:Acids; D007854:Lead