title: [Chromatographic fingerprint peak matching of Semen Cassiae by asymptotic window orthoganality projection analytical method].
abstract: OBJECTIVE
  To investigate the principle and basis about the chromatographic fingerprint peak matching of Semen Cassiae by the asymptotic window orthogonal projection analytical method.
  
  
  METHODS
  The samples of Semen Cassiae were hydrolyzed in the 1.5 mol x L(-1) hydrochloride acid and then reflux extracted with chloroform. The chromatographic condition was that the HPLC was run on Agilent 1100 column, Zorbax Eclipse XDB-C18 (4.6 mm x 250 mm, 5 microm). The protected column was Scienhome C18 column (3.0 mm x 20 mm, 5 microm) with acetonitrile-water (0.1% phosphoric acid) as mobile phase in gradient elution at a flow rate of 1.0 mL x min(-1). The detection wavelength and reference wavelength were 278 nm and 550 nm, respectively.
  
  
  RESULTS
  The characteristic fingerprint collection of illustrative plates of Semen Cassiae were obtained with different HPLC instruments and chromatographic columns. And the asymptotic window orthogonal projection analytical method was used to decide the chromatographic fingerprint peak matching of Semen Cassiae, promptly and prapidly.
  
  
  CONCLUSIONS
  The asymptotic window orthogonal projection analytical method can progress the matching of different fingerprint collection of illustrative plates peak matching well and truly.
authors.affiliation: Department of Pharmacy, Wannan Medical College, Wuhu 241002, China. zouchc@163.com
authors.forename: Chuncai
authors.initials: C
authors.lastname: Zou
authors.forename: Haiyan
authors.initials: H
authors.lastname: Yan
authors.forename: Hongzhuang
authors.initials: H
authors.lastname: Fang
mesh_terms: D002845:Chromatography / Q000379:methods*; D032904:Cinnamomum aromaticum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal