[Effects of RNA on blood circulation and its adrenergic and cholinergic regulation].
Experimental investigations of the impacts of microRNA agent nucleks on heart function, coronary and systemic circulation as well as on adrenergic and cholinergic mechanisms of cardiohaemodynamics regulation were performed on anaesthetized dogs. Bolus injection (0.1-100.0 mg) or prolonged infusion (2.5 mg/min) of nucleks into perfusion coronary artery blood stream induced coronary dilatation. Under the intracoronary infusion of nucleks we observed more pronounced coronary vasodilatation and left ventricle pressure elevation in response to adrenergic heart receptors stimulation by norepinephrine (0.05-5.0 mkg, intracoronary). Besides that the drug infusion into coronary blood stream promoted the acceleration of recovery processes of the studied cardiohaemnodynamic parameters after norepinephrine injection. After intracoronary infusion of nucleks the sensitivity of cholinergic receptors to the stimulation by acetylcholine (0.001-1.0 mkg, intracoronary) increased significantly. After NO-synthase blockade (L-NAME, infusion 2.0 mg/min, intracoronary) nucleks did not cause any effect on coronary vessels tone and heart activity both it did not change their adrenergic and cholinergic reactivity.
Neshcheret
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OP
Tkachuk
Z Iu
ZIu
Moĭbenko
O O
OO
D000109:Acetylcholine / Q000494:pharmacology; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D018678:Cholinergic Agents / Q000494:pharmacology; D003326:Coronary Circulation / Q000187:drug effects*; D004285:Dogs; D005260:Female; D006439:Hemodynamics / Q000187:drug effects*; D008297:Male; D035683:MicroRNAs / Q000008:administration & dosage / Q000494:pharmacology*; D009206:Myocardium / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009638:Norepinephrine / Q000494:pharmacology; D011941:Receptors, Adrenergic / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000378:metabolism*
D000316:Adrenergic alpha-Agonists; D018678:Cholinergic Agents; D035683:MicroRNAs; D011941:Receptors, Adrenergic; D011950:Receptors, Cholinergic; D019001:Nitric Oxide Synthase; D000109:Acetylcholine; D019331:NG-Nitroarginine Methyl Ester; D009638:Norepinephrine