title: [Study on the optimal extraction process of chaihushugan powder].
abstract: OBJECTIVE
  To study the optimal extraction process of chaihushugan powder by orthogonal design.
  
  
  METHODS
  RP-HPLC method was developed for the determination of saikosaponin a, ferulic acid, hesperidin and paeoniflorin in chaihushugan powder. The contents of the components and the extraction yield were selected as assessment indices. Four factors were study by L9 (3(4)), including the alcohol concentration, amount of alcohol, duration of extraction and times of extraction.
  
  
  RESULTS
  The optimal extracting condition was 80% alcohol consumed as 10 times of crude herb amount, and extracting two times for 90 min each time.
  
  
  CONCLUSIONS
  This study supplies theoretical base for the development of chaihushugan powder formulation.
authors.affiliation: Department of Pharmanacy, Hebei North College, Zhangjiakou 075000, China. wangxiaoya051001@126.com
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Wang
authors.forename: Wan-ming
authors.initials: WM
authors.lastname: Zhang
authors.forename: Dan-shen
authors.initials: DS
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: An
authors.forename: Jia-ming
authors.initials: JM
authors.lastname: Tian
mesh_terms: D001565:Benzoates / Q000032:analysis / Q000302:isolation & purification; D001952:Bridged-Ring Compounds / Q000032:analysis / Q000302:isolation & purification; D029972:Bupleurum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis / Q000302:isolation & purification; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification; D006569:Hesperidin / Q000032:analysis / Q000302:isolation & purification; D006358:Hot Temperature; D039821:Monoterpenes; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D011208:Powders; D011786:Quality Control; D012503:Saponins / Q000032:analysis* / Q000302:isolation & purification; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D003373:Coumaric Acids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D000431:Ethanol; D005960:Glucosides; D006569:Hesperidin; D039821:Monoterpenes; D009828:Oleanolic Acid; D011208:Powders; D012503:Saponins; C015423:peoniflorin; D006027:Glycosides; C004999:ferulic acid; C025759:saikosaponin D