[Study on the extraction technology of polysaccharides from Caulis Marsdeniae Tenacissimae].
OBJECTIVE
To optimize the extraction technology and to establish the determination method of polysaccharides from Caulis Marsdeniae Tenacissimae.


METHODS
Phenol-sulphate colorimetry was used to determine the content of polysaccharides. According to the yield of polysaccharides, the orthogonal design was used to select the extraction technology of polysaccharides.


RESULTS
The optimum extraction procedure was 8 folds of water, refluxing and extracting for 3 times at 100 degrees C, and 1 h for each time. The concentration of polysaccharide was linear in the range of 0.004-0.014 mg/mL The average recovery was 99.72%, RSD = 0. 63%.


CONCLUSIONS
The optimum extraction procedure is simple and reliable which is suitable for industrial production. The method for determination is simple, specific and accurate which can provide a method for mass control of Marsdenia tenacissima.
Li
Bin
B
College of Pharmacognosy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. eleven_lb@126.com
Liu
Xin-yun
XY
Zhang
Hui
H
Kang
Ting-guo
TG
D000970:Antineoplastic Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D029062:Apocynaceae / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014867:Water
D000970:Antineoplastic Agents; D011134:Polysaccharides; D012997:Solvents; D014867:Water