Measurement of transport activities of bile acids in human multidrug resistance-associated protein 3 using liquid chromatography-tandem mass spectrometry.
A method has been developed for the measurement of transport activities in membrane vesicles obtained from human multidrug resistance-associated protein 3-expressing Sf9 cells for 1beta-hydroxy-, 6alpha-hydroxy- and unsaturated bile acids by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. Calibration curves for the bile acids were linear over the range of 10 to 2000 pmol/mL, and the detection limit was less than 2 pmol/mL for all bile acids using selected reaction monitoring analysis. The method was applied to measurements of adenosine triphosphate-dependent transport activities of the membrane vesicles for the above-mentioned hydroxylated and unsaturated bile acids. The present study demonstrated that the human multidrug resistance-associated protein 3 vesicles accepted 1beta-, 6alpha-hydroxylated and unsaturated bile acids along with common bile acids, such as glycocholic acid and taurolithocholic acid 3-sulfate. The developed method is useful for measurements of bile acid transport activities.
Yamaguchi
Kana
K
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
Murai
Tsuyoshi
T
Yabuuchi
Hikaru
H
Kurosawa
Takao
T
D001647:Bile Acids and Salts / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008567:Membranes, Artificial; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000737:chemistry*; D013499:Surface Properties; D053719:Tandem Mass Spectrometry
D001647:Bile Acids and Salts; D008567:Membranes, Artificial; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C114462:multidrug resistance-associated protein 3