title: Flow cytometry vs cytomorphology for the detection of hematologic malignancy in body cavity fluids.
abstract: Flow cytometry and cytomorphology results on 92 body cavity fluids [61 effusions and 31 bronchoalveolar lavage fluids (BALF)] from hematologic malignancy were compared with retrospective clinical outcome. We observed double true positive/negative results in 67 cases (73%), and double false negative results in 2 cases (2%). Immunophenotyping accounted for true positive/negative results in 22 out of 23 mismatched cases (25%), and retained significantly higher accuracy than that of cytomorphology especially in effusions and differentiated lymphoma. In BALF analysis, immunophenotyping and cytomorphology sensitivity was 75% and 0%, respectively. Flow cytometry retains the highest accuracy in detecting neoplastic cells in body cavity fluids.
authors.affiliation: Department of Transfusion Medicine, Division of Hematology, Ospedale Niguarda Ca' Granda Hospital, Piazza Ospedale Maggiore, 3, 20162 Milan, Italy.
authors.forename: Clara
authors.initials: C
authors.lastname: Cesana
authors.forename: Catherine
authors.initials: C
authors.lastname: Klersy
authors.forename: Barbara
authors.initials: B
authors.lastname: Scarpati
authors.forename: Bruno
authors.initials: B
authors.lastname: Brando
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Volpato
authors.forename: Giambattista
authors.initials: G
authors.lastname: Bertani
authors.forename: Maurizio
authors.initials: M
authors.lastname: Faleri
authors.forename: Annamaria
authors.initials: A
authors.lastname: Nosari
authors.forename: Silvia
authors.initials: S
authors.lastname: Cantoni
authors.forename: Ursula
authors.initials: U
authors.lastname: Ferri
authors.forename: Linda
authors.initials: L
authors.lastname: Scampini
authors.forename: Claudia
authors.initials: C
authors.lastname: Barba
authors.forename: Giuliana
authors.initials: G
authors.lastname: Lando
authors.forename: Enrica
authors.initials: E
authors.lastname: Morra
authors.forename: Roberto
authors.initials: R
authors.lastname: Cairoli
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor; D001826:Body Fluids; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002452:Cell Count; D005189:False Positive Reactions; D005260:Female; D005434:Flow Cytometry; D019337:Hematologic Neoplasms / Q000175:diagnosis*; D006801:Humans; D016130:Immunophenotyping; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012189:Retrospective Studies
chemical_list: D014408:Biomarkers, Tumor