title: [Technical condition of semi-bionic extraction in optimizing formula of Rhizoma Cyperi that had been extracted by SFE-CO2 through homogeneous design].
abstract: OBJECTIVE
  To optimize the extraction condtions in the modification of Rhizoma Cyperi, which had been extracted by the technique of supercritical CO2 extraction.
  
  
  METHODS
  The semi-bionic extraction (SBE) conditions were optimized through homogeneous design while the extracts (< or = 1 000), the total area of HPLC and dry extracts were adopted as markers.
  
  
  RESULTS
  The optimized SBE extraction conditions are the following: pH in first extraction was adjusted to 2.005 3. And then pH in second and third extraction adjusted to 6.508 2 and 8.945 6, time of the whole extracted process was 3.912 7 h.
  
  
  CONCLUSIONS
  Combine the faction of production,we make sure the conclusion is pH in first extraction was 2.00, pH in second and third extraction adjusted to 6.50 and 9.00, duration of run was 2.0 h, 1.0 h and 1.0 h, respectively.
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan 250355, China. sunxiumei8@163.com
authors.forename: Xiumei
authors.initials: X
authors.lastname: Sun
authors.forename: Haiyan
authors.initials: H
authors.lastname: Shi
authors.forename: Zhaowang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D029786:Cyperus / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D027343:Rhizome / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D002245:Carbon Dioxide