title: Effect of calcitonin gene-related peptide on the expression of triggering receptor expressed on myeloid cells-1 in lipopolysaccharide-induced macrophages.
abstract: OBJECTIVE
  To determine the effect of calcitonin gene-related peptide (CGRP) on triggering receptor expressed on myeloid cells-1 (TREM-1) in the lipopolysaccharide (LPS)-induced macrophages and its signal transduction pathway.
  
  
  METHODS
  The levels of TREM-1 mRNA in the macrophages were observed by reverse transcription-polymerase chain reaction (RT-PCR), and flow cytometry was performed to detect TREM-1 protein expression levels in the macrophages.
  
  
  RESULTS
  CGRP had no regulating effect on the expression of TREM-1 in the macrophages; LPS could up-regulate macrophages to express TREM-1; CGRP increased TREM-1 mRNA expression in LPS-induced macrophages in dose and time-dependent manner; CGRP increased TREM-1 protein expression in LPS-induced macrophages, which could be partially reversed by H-7 or H-89 (P<0.05).
  
  
  CONCLUSIONS
  CGRP can regulate the LPS-induced macrophages synthesis and secretion of TREM-1, and the intracellular signal transduction pathway is related to PKA and PKC.
authors.affiliation: Department of Clinical Medicine, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Yunchao
authors.initials: Y
authors.lastname: Li
authors.forename: Chaxiang
authors.initials: C
authors.lastname: Guan
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Guoying
authors.initials: G
authors.lastname: Sun
authors.forename: Meng
authors.initials: M
authors.lastname: Shi
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Wenjie
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002460:Cell Line; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011494:Protein Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011971:Receptors, Immunologic; C517262:TREM1 protein, mouse; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D011494:Protein Kinases; D015740:Calcitonin Gene-Related Peptide