Urea-gradient protein refolding in size exclusion chromatography.
Protein refolding using urea gradient size exclusion chromatography (UGSEC) is a new version of conventional SEC refolding, which incorporates urea gradient into the SEC. Operating factors, mainly the urea gradient length and the final urea concentration in the gradient, are discussed in detail. Recent applications of UGSEC, including refolding of recombinant human granulocyte colony stimulating factor by UGSEC, is shown as an example.
Wang
Chaozhan
C
Institute of Modern Separation Science, College of Chemistry and Materials Science, Northwest University, 229 TaiBai North Road, Xi'an 710069, P R China. czwang@nwu.edu.cn
Cheng
Yan
Y
D002850:Chromatography, Gel / Q000379:methods*; D006801:Humans; D002479:Inclusion Bodies / Q000737:chemistry*; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D014508:Urea / Q000737:chemistry*
D011994:Recombinant Proteins; D014508:Urea