title: Inhibition of degranulation and cytokine production in bone marrow-derived mast cells by hydrolyzed rice bran.
abstract: OBJECTIVE
  We investigated the effects of hydrolyzed rice bran (HRB), an arabinoxylan extracted from rice bran, on mast cell degranulation and cytokine production.
  
  
  METHODS
  HRB was obtained by treating rice bran with an extract obtained from shiitake mushrooms. Bone marrow-derived mast cells (BMMCs) were prepared by culturing bone marrow cells from BALB/c mice in the presence of interleukin-3 and stem cell factor for 4 weeks. BMMCs were pretreated with HRB (0-3 mg/ml) for 30 min and were then antigen activated.
  
  
  RESULTS
  Pretreatment of BMMCs with HRB significantly inhibited antigen-induced degranulation and cytokine production (tumor necrosis factor-alpha and interleukin-4) in a dose-dependent manner. HRB also diminished membrane fusion between liposomes in which soluble N-ethyl maleimide-sensitive factor attachment protein receptors were reconstituted. Phosphorylation of RelA and mitogen-activated kinases after antigen stimulation was suppressed by pretreatment of BMMCs with HRB.
  
  
  CONCLUSIONS
  These findings suggest that HRB may have an anti-inflammatory effect by inhibiting mast cell degranulation and cytokine production.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Naohide
authors.initials: N
authors.lastname: Hirashima
authors.forename: Mamoru
authors.initials: M
authors.lastname: Nakanishi
authors.forename: Tadahide
authors.initials: T
authors.lastname: Furuno
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D015550:Cell Degranulation / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D012275:Oryza / Q000033:anatomy & histology / Q000276:immunology*; D051381:Rats; D017455:Receptors, IgE / Q000378:metabolism; D050600:SNARE Proteins / Q000378:metabolism; D014990:Xylans / Q000276:immunology*
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D017455:Receptors, IgE; D050600:SNARE Proteins; D014990:Xylans; D007073:Immunoglobulin E; C085118:arabinoxylan; D020928:Mitogen-Activated Protein Kinases; D002118:Calcium