Isolation and culture of embryonic pancreas and liver.
Culturing embryonic tissue in an in vitro setting offers the unique ability to manipulate the external medium and therefore to investigate the pathways involved in regulating normal organogenesis as well as providing models for developmental disorders. Here we describe a system for the in vitro culture of the dorsal pancreatic buds and liver buds from mouse embryos. The tissues are dissected from day 9.0 or 11.5 mouse embryos. The tissues are placed on fibronectin-coated coverslips in serum-containing medium and allowed to attach. Over the next few days, the buds grow as flattened structures which are thin enough to allow the use of wholemount immunostaining methods.
Burke
Zoë D
ZD
Department of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath, Bath BA2 7AY, UK.
Li
Wan-Chun
WC
Slack
Jonathan M W
JM
Tosh
David
D
D000818:Animals; D004210:Dissection; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D051379:Mice; D010179:Pancreas / Q000166:cytology / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D011247:Pregnancy; D013194:Staining and Labeling; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000379:methods*
D005353:Fibronectins