title: Pig large tumor suppressor 2 (Lats2), a novel gene that may regulate the fat reduction in adipocyte.
abstract: Clenbuterol, a Beta(2)-adrenoceptor agonist, has been proven to be a powerful repartition agent that can decrease fat deposition. Based on results from our previous cDNA microarray experiment of pig clenbuterol administration, a novel up-regulated EST was full-length cloned (4859 bp encoding 1041 amino acids) and found to be the pig homolog of large tumor suppressor 2 (Lats2). We mapped pig Lats2 to chromosome 11p13-14 by using FISH, and western blotting demonstrated that pig Lats2 protein was most abundant in adipose. In Drosophila, Lats2 ortholog was reported as a key component of the Hippo pathway which regulates cell differentiation and growth. Here, we show that pig Lats2 exhibit inverted expression to YAP1, another member of the Hippo pathway which positively regulates cell growth and proliferation, during the differentiation of 3T3-L1 preadipocytes. Our results suggested that Lats2 may involve in Hippo pathway regulating the fat reduction by inhibiting adipocyte differentiation and growth.
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: QiuYue
authors.initials: Q
authors.lastname: Liu
authors.forename: XiaoRong
authors.initials: X
authors.lastname: Gu
authors.forename: YiQiang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: YaoFeng
authors.initials: Y
authors.lastname: Zhao
authors.forename: QingYong
authors.initials: Q
authors.lastname: Meng
authors.forename: GuoHeng
authors.initials: G
authors.lastname: Xu
authors.forename: XiaoXiang
authors.initials: X
authors.lastname: Hu
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D002976:Clenbuterol / Q000494:pharmacology; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism; D005223:Fats / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D015398:Signal Transduction; D013552:Swine; D025521:Tumor Suppressor Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D000318:Adrenergic beta-Agonists; D029721:Drosophila Proteins; D005223:Fats; D025521:Tumor Suppressor Proteins; D002976:Clenbuterol