Note: Application of laser produced plasma K alpha x-ray probe in radiation biology.
A dedicated radiation biology x-ray generation and exposure system has been developed. 8.0 keV in energy x-ray pulses generated with a femtosecond-laser pulse was used to irradiate sample cells through a custom-made culture dish with a silicon nitride membrane. The x-ray irradiation resulted in DNA double-strand breaks in the nucleus of a culture cell that were similar to those obtained with a conventional x-ray source, thus demonstrating the feasibility of radiobiological studies utilizing a single burst of x-rays focused on single cell specimens.
Nishikino
Masaharu
M
Quantum Beam Science Directorate, Japan Atomic Energy Agency, Advanced Photon Research Center, 8-1-7 Umemidai, Kizugawa, Kyoto 619-0215, Japan. nishikino.masaharu@jaea.go.jp
Sato
Katsutoshi
K
Hasegawa
Noboru
N
Ishino
Masahiko
M
Ohshima
Shinsuke
S
Okano
Yasuaki
Y
Kawachi
Tetsuya
T
Numasaki
Hodaka
H
Teshima
Tetruki
T
Nishimura
Hiroaki
H
D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D003300:Copper; D006657:Histones / Q000378:metabolism; D006801:Humans; D007834:Lasers; D011829:Radiation Dosage; D011853:Radiobiology / Q000295:instrumentation*; D014965:X-Rays
C468783:H2AX protein, human; D006657:Histones; D003300:Copper