title: [Molecular epidemiological survey on HIV-1 among the injecting drug users population in Shenzhen].
abstract: OBJECTIVE
  To study the prevalent status of human immunodeficiency virus-1 (HIV-1) subtypes in IDU (injecting drug users) population in Shenzhen and to study their source of infection in order to predict the epidemic trend and evolution.
  
  
  METHODS
  166 HIV-1 positive plasma from the IDUs was collected from 1996 to 2008. HIV-1 env genes were amplified by nested-PCR from RNA. The C2-V3 regions (450 bp) of HIV-1 env were sequenced for analyses. Phylogenetic analyses were performed on the nucleotide sequence data.
  
  
  RESULTS
  Among 166 samples, there were 6 HIV-1 strains including CRF01_AE, CRF08_BC, CRF07_BC 3 circulating recombinant forms (CRFs) and B', C, A1 3 subtypes. Data from the genotype analyses showed that 65.06% (108/166) were CRF01_AE, 19.88% (33/166) were CRF07_BC, 6.02% (10/166) were CRF08_BC, 7.23% (9/166) were subtype B', 0.60% (1/166) were subtype C and 1.20% (2/166) were subtype A1. Phylogenetic tree analysis showed that some of HIV-1 clusters defined in CRF01_AE, CRF07_BC and subtype B' in different time groups. Significant increase of gene distance in CRF01_AE and CRF07_BC strains in the three different periods.
  
  
  CONCLUSIONS
  CRF01_AE and CRF07_BC were the major epidemic CRF strains among the IDU population in Shenzhen while the subtype B', C, A1 and CRF08_BC were also circulating in IDU population in this region. The variation of all different subtypes was increasing through these years.
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
authors.forename: Guang-lu
authors.initials: GL
authors.lastname: Zhao
authors.forename: Tie-jian
authors.initials: TJ
authors.lastname: Feng
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Shi
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yong-xia
authors.initials: YX
authors.lastname: Gan
authors.forename: Xian-ru
authors.initials: XR
authors.lastname: Luo
mesh_terms: D002681:China / Q000453:epidemiology; D055030:Drug Users; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny