title: Automatic fluorescence microphotometer using an image dissector tube.
abstract: An automatic fluorescence microphotometer using an image dissector tube as the fluorescence detector is described. The image dissector tube is used in the photon-counting mode of operation to allow precise intensity measurements of weak fluorescent spots. With the support of a laboratory-built microcomputer system, the instrument can automatically search for cells stained with fluorescent dye in the microscope field; then the detected cells are individually put in the measuring probe region to have their fluorescence intensities measured. Finally a fluorescence distribution histogram of stained cells is automatically generated and graphically presented. The instrument also allows a fluorescence intensity mapping operation utilizing the conventional raster scanning technique. To demonstrate the system's performance, DNA in rat liver nuclei stained with Feulgen dye was measured.
authors.forename: S
authors.initials: S
authors.lastname: Wungkobkiat
authors.forename: T
authors.initials: T
authors.lastname: Morita
authors.forename: T
authors.initials: T
authors.lastname: Uesaka
authors.forename: S
authors.initials: S
authors.lastname: Minami