[Immunological killing effect of recombicant adenovirus vector rAD-mTERT-m4-1BBL on mouse hepatoma cell line Hepa1-6 cells co-cultured with T lymphocytes].
OBJECTIVE
To study the immunological suppressing effect of recombinant adenovirus vector rAD-mTERT promotor-m4-1BBL (rAD-mTERT) on mouse hepatoma cell line Hepa1-6 cells in co-culture with T lymphocytes.


METHODS
Adding recombinant adenovirus rAD, rAD-CMV-m4-1BBL (rAD-CMV) and rAD-mTERT to Hepa1-6 and L929 cells, respectively, to observe the effect of these adenoviruses on growth and apoptosis of these cells in co-culture with T lymphocytes.


RESULTS
Adding adenovirus significantly suppressed the growth and slightly increased apoptosis of the two types of cells (P < 0.05). rAD-mTERT promotor-m4-1BBL showed only pro-apoptotic effect on Hepa1-6 cells. When co-cultured with T lymphocytes, rAD-CMV-m4-1BBL showed promoting effect on apoptosis of the cells. Compared with that of T cells pre-co-culture, CD4(+) and CD8(+) T cells were proliferated, and the ratio of CD4/CD8 was significantly reduced (from 1.27 to 1.08).


CONCLUSIONS
Adding the recombinant adenoviruses only suppresses the cell growth, but not promotes their apoptosis. In co-culture with T lymphocytes, recombinant adenovirus vector rAD-mTERT promotor-m4-1BBL can targetingly suppress the growth and induce apoptosis of Hepa1-6 cells. The apoptosis is induced through the immunological killing effect of T lymphocytes.
Xiao
Zhang-sheng
ZS
Department of General Surgery, Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
Ma
Shi-yin
SY
Gong
Wei-da
WD
Yao
Hui-hua
HH
DU
Peng
P
Xing
Ying-qing
YQ
Wu
Hao-rong
HR
D053330:4-1BB Ligand / Q000235:genetics / Q000502:physiology*; D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis; D016516:CD4-CD8 Ratio; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018920:Coculture Techniques; D005347:Fibroblasts / Q000166:cytology; D005822:Genetic Vectors; D008114:Liver Neoplasms, Experimental / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology*; D019098:Telomerase / Q000235:genetics*; D014162:Transfection
D053330:4-1BB Ligand; D011994:Recombinant Proteins; D019098:Telomerase