[Assay of evodin, evodiamine and rutaecarpine in Fructus Evodiae by QAMS].
OBJECTIVE
To develop a Quantitative Assay of Multi-components by Single - marker (QAMS) for simultaneous determination of three components in Fructus Evodiae, and examine the feasibility of using the relative correction factors between the different types of compounds.


METHODS
Rutaecarpine was selected as the internal reference substance; the relative correction factors of evodin and evodiamine were calculated. The contents of three components in 11 batches of samples were determined by both external standard method and QAMS. The validity of the QAMS method was evaluated by comparison of their quantitative results.


RESULTS
No obvious differences (RSD < 5%) were found in the quantitative results of evodin and evodiamine in 11 batches of Fructus Evodiae determined by the two methods.


CONCLUSIONS
It is feasible and suitable to determine evodin and evodiamine in Fructus Evodiae by QAMS, and this method can be used for a certain different types of compounds.
Song
Yafang
Y
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Wang
Zhimin
Z
Zhu
Jingjing
J
Yan
Lihua
L
Zhang
Qiwei
Q
Gong
Muxin
M
Gao
Huimin
H
Feng
Weihong
W
Wang
Weihao
W
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D032086:Evodia / Q000737:chemistry*; D005663:Furans / Q000032:analysis*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000032:analysis*; D026121:Indole Alkaloids / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D011799:Quinazolines / Q000032:analysis*; D052999:Quinazolinones
D004365:Drugs, Chinese Herbal; D005663:Furans; D006576:Heterocyclic Compounds, 4 or More Rings; D026121:Indole Alkaloids; D010936:Plant Extracts; D011799:Quinazolines; C473746:evodine; C028632:rutecarpine; D052999:Quinazolinones; C049639:evodiamine