title: The assay of pterostilbene in spiked matrices by liquid chromatography tandem mass spectrometry and isotope dilution method.
abstract: Pterostilbene (trans-3,5-dimethoxy-4-hydroxystilbene) is an active component found in several plant species, exhibiting important pharmacological properties. A new and reliable method of assaying this phyto compound in various matrices is presented; the assay is based on (1) the selectivity of liquid chromatography (LC) hyphenated with electrospray ionisation (ESI), (2) the specificity of a two-step mass spectrometric analysis (MS/MS) and (3) the accuracy of the isotope dilution method. The labelled analogue may be conveniently synthesised in a few steps. The sensitivity of the method is confirmed by the very low limit of detection (LOD) and limit of quantitation (LOQ) values achieved in the assay of pterostilbene in two distinct fortified matrices, and is further supported by the observed accuracy values.
authors.affiliation: Dipartimento di Chimica, Università della Calabria, cubo 12/C, I-87036 Arcavacata di Rende (CS), Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Mazzotti
authors.forename: Leonardo
authors.initials: L
authors.lastname: Di Donna
authors.forename: Hicham
authors.initials: H
authors.lastname: Benabdelkamel
authors.forename: Bartolo
authors.initials: B
authors.lastname: Gabriele
authors.forename: Anna
authors.initials: A
authors.lastname: Napoli
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sindona
mesh_terms: D029798:Blueberry Plants / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013267:Stilbenes / Q000032:analysis* / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D013267:Stilbenes; C107773:pterostilbene