title: Effect of fluoride-substituted apatite on in vivo bone formation.
abstract: Biological apatites are characterized by the presence of minor constituents such as magnesium (Mg), chloride (Cl), or fluoride (F) ions. These ions affect cell proliferation and osteoblastic differentiation during bone tissue formation. F-substituted apatites are being explored as potential bonegraft materials. The aim of the present study is to investigate the mechanism of bone formation induced by fluoride-substituted apatite (FAp) by analyzing the effect of FAp on the process of in vivo bone formation. FAps containing different F concentrations (l-FAp: 0.48 wt%, m-FAp: 0.91 wt%, h-FAp: 2.23 wt%) and calcium-deficient apatite (CDA), as positive control, were implanted in rat tibia and bone formation was evaluated by histological examination, immuhistochemistry, in situ hybridization and tartrate-resistant acid phosphatase examinations. The results showed that l-FAp, m-FAp, h-FAp, and CDA biomaterials allowed migration of macrophages, attachment, proliferation, and phenotypic expression of bone cells leading to new bone formation in direct apposition to the particles. However, the l-FAp preparation allowed faster bone conduction compared to the other experimental materials. These results suggest that FAp with low F concentration may be an efficient bonegraft material for dental and medical application.
authors.affiliation: Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Inoue
authors.forename: Andrea P
authors.initials: AP
authors.lastname: Rodriguez
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nagai
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagatsuka
authors.forename: Racquel Z
authors.initials: RZ
authors.lastname: LeGeros
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Tsujigiwa
authors.forename: Masahisa
authors.initials: M
authors.lastname: Inoue
authors.forename: Etsuo
authors.initials: E
authors.lastname: Kishimoto
authors.forename: Shin
authors.initials: S
authors.lastname: Takagi
mesh_terms: D000818:Animals; D001031:Apatites / Q000008:administration & dosage* / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry*; D001842:Bone and Bones / Q000187:drug effects; D002118:Calcium / Q000008:administration & dosage / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D005459:Fluorides / Q000008:administration & dosage* / Q000737:chemistry*; D010010:Osteoclasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D013977:Tibia / Q000187:drug effects / Q000378:metabolism
chemical_list: D001031:Apatites; D018786:Bone Substitutes; D005459:Fluorides; D002118:Calcium