Genomic structure of an economically important cyanobacterium, Arthrospira (Spirulina) platensis NIES-39.
A filamentous non-N(2)-fixing cyanobacterium, Arthrospira (Spirulina) platensis, is an important organism for industrial applications and as a food supply. Almost the complete genome of A. platensis NIES-39 was determined in this study. The genome structure of A. platensis is estimated to be a single, circular chromosome of 6.8 Mb, based on optical mapping. Annotation of this 6.7 Mb sequence yielded 6630 protein-coding genes as well as two sets of rRNA genes and 40 tRNA genes. Of the protein-coding genes, 78% are similar to those of other organisms; the remaining 22% are currently unknown. A total 612 kb of the genome comprise group II introns, insertion sequences and some repetitive elements. Group I introns are located in a protein-coding region. Abundant restriction-modification systems were determined. Unique features in the gene composition were noted, particularly in a large number of genes for adenylate cyclase and haemolysin-like Ca(2+)-binding proteins and in chemotaxis proteins. Filament-specific genes were highlighted by comparative genomic analysis.
Fujisawa
Takatomo
T
Bioresource Information Center, Department of Biotechnology, National Institute of Technology and Evaluation, 2-10-49 Nishihara, Shibuya-ku, Tokyo 151-0066, Japan.
Narikawa
Rei
R
Okamoto
Shinobu
S
Ehira
Shigeki
S
Yoshimura
Hidehisa
H
Suzuki
Iwane
I
Masuda
Tatsuru
T
Mochimaru
Mari
M
Takaichi
Shinichi
S
Awai
Koichiro
K
Sekine
Mitsuo
M
Horikawa
Hiroshi
H
Yashiro
Isao
I
Omata
Seiha
S
Takarada
Hiromi
H
Katano
Yoko
Y
Kosugi
Hiroki
H
Tanikawa
Satoshi
S
Ohmori
Kazuko
K
Sato
Naoki
N
Ikeuchi
Masahiko
M
Fujita
Nobuyuki
N
Ohmori
Masayuki
M
D001426:Bacterial Proteins / Q000235:genetics; D002874:Chromosome Mapping; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D012329:RNA, Bacterial / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D053545:Spirulina / Q000235:genetics*
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012343:RNA, Transfer