[Morphological observation of bovine kidney (MDBK) cells effected by foot-and-mouth disease virus L(pro)].
In order to explore the morphological changes of Bovine Kidney (MDBK) cells induced by foot-and-mouth disease virus (FMDV) L protease, we induced the expression of FMDV L protease in bovine kidney cells (MDBK) artificially. All work is carried out on the basis of a stable MDBK cell line inducibly expresses the Lab gene under the control of tetracycline. We use cell morphology, Hoechst 33258 staining, AO-EB staining, and DNA Ladder abstraction to research the morphological changes of MDBK cells. 24 hours after FMDV L protease were induced and expressed in MDBK cells, cells shown the diminish of cell size, nuclear enrichment and the appearance of transparency circle under the light microscope. Apoptosis characteristics of nuclear condensation, fragmentation, accompanied by apoptotic bodies formation (Hoechst 33258 staining). 36 hours after the expression, nuclear staining of early lesions showed bright green plaque or debris-like dense, and advanced lesions showed Orange and dense plaques (AO-EB staining). 48 hours after the expression, DNA gel electrophoresis showed visible DNA ladder. Results indicate that FMDV L protease can induce apoptosis of MDBK and apoptosis plays an important role in the cytopathogenicity effect of FMDV.
Hao
Fengqiang
F
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou 730046, China.
Cong
Guozheng
G
Gao
Shandian
S
Lin
Tong
T
Du
Junzheng
J
Shao
Junjun
J
Chang
Huiyun
H
D000818:Animals; D002417:Cattle; D002460:Cell Line; D010450:Endopeptidases / Q000096:biosynthesis* / Q000235:genetics; D029544:Foot-and-Mouth Disease Virus / Q000472:pathogenicity*; D007668:Kidney / Q000166:cytology* / Q000473:pathology* / Q000821:virology; D014162:Transfection
D010450:Endopeptidases; C088747:leader proteinase, foot-and-mouth disease virus