[Meniscal degeneration score and NITEGE expression : immunohistochemical detection of NITEGE in advanced meniscal degeneration].
BACKGROUND
Meniscal degeneration (MD) is a structural change of fibrous cartilage that is common in orthopaedic diagnostics and relevant for health insurance matters. So far, there has been neither a standardised scoring system nor an immunohistochemical marker for MD.


METHODS
In this retrospective trial, the meniscal tissue of 60 patients was assessed immunohistochemically for NITEGE (G1 fragment of the proteoglycan aggrecan) expression. NITEGE expression was correlated with defined grades of MD: little (grade 0/1), medium (grade 2), or severe (grade 3).


RESULTS
Detection of extracellular NITEGE deposits in grade 2 or 3 MD had a positive predictive value and specificity of 100%, whereas no deposits were found in grade 0/1 MD. Sensitivity in advanced MD was 55%. Detection of extracellular NITEGE correlated positively with the grade of degeneration, as did patient age and the grade of degeneration. The patient age of those with grade 0/1 MD was significantly lower than for grade 3 (p<0.0001).


CONCLUSIONS
The thoroughly defined degeneration score (grade 1 - grade 3 MD) is suitable to assess the severity of degeneration. Extracellular NITEGE deposits can be regarded as an immunohistochemical marker for advanced (grades 2 and 3) MD.
Krenn
V
V
Zentrum für Histologie, Zytologie und Molekulare Diagnostik, Max-Planck-Strasse 18+20, 54296, Trier, Deutschland. krenn@patho-trier.de
Knöss
P
P
Rüther
W
W
Jakobs
M
M
Otto
M
M
Krukemeyer
M G
MG
Heine
A
A
Möllenhoff
G
G
Kurz
B
B
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D015415:Biomarkers / Q000032:analysis; D002452:Cell Count; D048429:Cell Size; D019902:Chondrocytes / Q000473:pathology; D018450:Disease Progression; D010450:Endopeptidases / Q000032:analysis*; D005109:Extracellular Matrix / Q000473:pathology; D005260:Female; D006801:Humans; D007718:Knee Injuries / Q000473:pathology / Q000601:surgery; D008297:Male; D008592:Menisci, Tibial / Q000473:pathology* / Q000601:surgery; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000145:classification / Q000175:diagnosis / Q000473:pathology*; D011237:Predictive Value of Tests; D012038:Regeneration / Q000502:physiology; D013583:Synovial Membrane / Q000473:pathology; D000070600:Tibial Meniscus Injuries; D055815:Young Adult
D015415:Biomarkers; D010450:Endopeptidases; C083641:aggrecanase