[Laboratory diagnostics and monitoring of virus circulation in surveillance system for rubella in Belarus].
OBJECTIVE
To summarize data on laboratory diagnostics of prenatal and postnatal rubella and molecular monitoring of rubella virus circulation in Belarus obtained during implementation of rubella elimination program.


METHODS
Serum samples from 2314 persons were tested on the presence of IgM to rubella virus and measles virus (in case of negative result on rubella) using respective enzyme immunoassays. Virological testing using RT-PCR as well as genotyping on the basis of E1 gene fragment sequencing were also performed. Two viruses isolated in Belarus were set as reference strains of genotypes 1G and 1h.


RESULTS
Implementation of laboratory diagnostics allowed to differentiate cases of rubella from other exanthematous infections, significantly increase the number of laboratory-confirmed cases among all reported cases, and show presence of endemic circulation of rubella virus strains of 3 different genotypes (1G, 1E, and 1h) in Belarus (2004-2006). In 2006, when relatively high incidence of rubella was reported in the country (24.39 per 100,000 population), the risk of congenital rubella syndrome was not less than 9 per 100,000 births. Conducted in October 2005-May 2006 additional rounds of immunization against rubella (>1 million people were vaccinated) decreased incidence to single cases.


CONCLUSIONS
Obtained data show achievability of indigenous rubella elemination by 2010. Revealed genetic diversity of rubella virus strains allowed to update the International classification of wild rubella viruses.
Samoĭlovich
E O
EO
Semeĭko
G V
GV
Ermolovich
M A
MA
Svirchevskaia
E Iu
EIu
D000914:Antibodies, Viral / Q000097:blood; D004784:Environmental Monitoring; D062665:Epidemiological Monitoring; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D017720:Molecular Epidemiology; D015397:Program Evaluation; D002094:Republic of Belarus / Q000453:epidemiology; D012409:Rubella / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology*; D012412:Rubella virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016036:Seroepidemiologic Studies; D014759:Viral Envelope Proteins / Q000235:genetics
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D014759:Viral Envelope Proteins; C061826:E1 envelope protein, Rubella virus