Aquilegia vulgaris extract protects against the oxidative stress and the mutagenic effects of cadmium in Balb/c mice.
Cadmium (Cd) is a non-essential element and is a widespread environmental pollutant. Exposure to cadmium can result in cytotoxic, carcinogenic and mutagenic effects. The aim of the current work was to evaluate the protective effect of Aquilegia vulgaris extract against the oxidative stress and the genotoxicity induced by Cd using the chromosomal aberrations in somatic and germ cells assay and random amplified polymorphism DNA (RAPD-PCR) analysis. Forty male Balb/c mice were divided into four groups including the control group, Cd-treated group and the groups treated with the extract alone or plus Cd. The results indicated that Cd increased serum ALT, AST, urea, LDH, CK, lipid peroxidation in liver tissue accompanied with a significant decrease in GPX and SOD. Cd also increased the number of chromosomal aberrations in bone marrow and spermatocytes including structural and numerical aberrations. Animals treated with the extract alone were comparable to the control regarding all the tested parameters. The extract succeeded in preventing or diminishing the oxidative stress and the clastogenic effects of Cd. It could be concluded that Aquilegia vulgaris extract is a promising protective agent against oxidative stress and genotoxicity during the exposure to Cd.
Abdel-Aziem
Sekena H
SH
Cell Biology Department, National Research Center, Dokki, Cairo, Egypt.
El-Nekeety
Aziza A
AA
Barakat
Ibrahim A
IA
Mohamed
Mohamed I
MI
Abdel-Wahhab
Mosaad A
MA
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D031884:Aquilegia; D002104:Cadmium / Q000633:toxicity; D002869:Chromosome Aberrations / Q000139:chemically induced / Q000187:drug effects; D004247:DNA / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D009153:Mutagens / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D019105:Random Amplified Polymorphic DNA Technique
D000975:Antioxidants; D009153:Mutagens; D010936:Plant Extracts; D002104:Cadmium; D004247:DNA