Effects of treadmill exercise on cyclooxygenase-2 in the hippocampus in type 2 diabetic rats: correlation with the neuroblasts.
Cyclooxygenase (COX) is a rate-limiting enzyme in synthesis of prostaglandins from arachidonic acid. In this study, we observed the effects of a physical exercise on COX-2 immunoreactivity in the hippocampus using immunohistochemistry in rats. In addition, we examined effects of administration of a COX-2 inhibitor, celecoxib, on neuroblast differentiation. At 6weeks of age, Zucker lean control (ZLC) and Zucker diabetic fatty (ZDF) rats were put on a treadmill with or without running for 1h/session/day for 5weeks. The running speed was gradually increased from 16 to 22m/min with 2m/min per 2weeks. In the ZLC and ZDF rats, COX-2 immunoreaction was detected in the granule cell layer of the dentate gyrus and in the stratum pyramidale of the CA2/3 region; COX-2 immunoreaction in the CA1 region was hardly detected. In the exercised-ZLC and ZDF rats, COX-2 immunoreactivity was significantly increased compared to that in the ZLC and ZDF rats, showing that COX-2 immunoreactivity in the exercised-ZDF rats was slightly low than that in the exercised-ZDF rats. In addition, weak COX-2 immunoreactivity was shown in the CA1 region by exercise. On the other hand, the repeated oral administration of celecoxib to 4-week-old ZDF rats significantly decreased the neuroblasts in the subgranular zone of the dentate gyrus. These results suggest that COX-2 may be associated with the increase of synaptic plasticity or contacts in the hippocampus.
Hwang
In Koo
IK
Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
Yi
Sun Shin
SS
Yoo
Ki-Yeon
KY
Park
Ok Kyu
OK
Yan
Bingchun
B
Kim
Il Yong
IY
Kim
Yo Na
YN
Song
Wook
W
Moon
Seung Myung
SM
Won
Moo-Ho
MH
Seong
Je Kyung
JK
Yoon
Yeo Sung
YS
D000818:Animals; D002450:Cell Communication / Q000502:physiology; D051546:Cyclooxygenase 2 / Q000378:metabolism* / Q000502:physiology; D048909:Diabetes Complications / Q000201:enzymology* / Q000473:pathology / Q000628:therapy*; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology* / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D005080:Exercise Test; D006624:Hippocampus / Q000201:enzymology*; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000201:enzymology / Q000473:pathology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D011924:Rats, Zucker; D013234:Stem Cells / Q000166:cytology / Q000201:enzymology / Q000473:pathology; D016896:Treatment Outcome
D051546:Cyclooxygenase 2