Peptide 68-88 of apocytochrome c plays a crucial role in its insertion into membrane and binding to mitochondria.
Apocytochrome c (Apocyt. c) is the precursor of cytochrome c. It is synthesized in the cytosol and posttranslationally imported into mitochondria. In order to determine the crucial sequence in apocyt. c translocation, deleted mutant and chemically synthesized peptides with different length were used. Obtained results showed that sequence 68-88 of apocyt. c plays a critical role in its insertion into membrane and binding to mitochondria.
Wang
Xiaoping
X
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Han
Xuehai
X
Yang
Fuyu
F
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002645:Chickens; D045304:Cytochromes c / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D008563:Membrane Lipids / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion
D030161:Avian Proteins; D008563:Membrane Lipids; D010446:Peptide Fragments; D011994:Recombinant Proteins; D045304:Cytochromes c