Cyclic mechanical strain promotes transforming-growth-factor-beta1-mediated cardiomyogenic marker expression in bone-marrow-derived mesenchymal stem cells in vitro.
Cardiomyocytes in the heart reside in mechanically dynamic environments, such as those subject to cyclic mechanical strain. TGF-beta1 (transforming growth factor-beta1) stimulates cardiomyogenic marker expression of BMMSCs (bone-marrow-derived mesenchymal stem cells). In the present study, we tested the hypothesis that cyclic mechanical strain promotes TGF-beta1-mediated cardiomyogenic marker expression in BMMSCs in vitro. The mRNA expression of cardiac-specific genes was more up-regulated in BMMSCs cultured with a TGF-beta1 supplement and subjected to cyclic strain for 1 week than in BMMSCs cultured statically with a TGF-beta1 supplement. Immunocytochemical analyses and flow cytometric analysis showed that the proportions of cardiac troponin-I-positive cells and cardiac MHC (myosin heavy chain)-positive cells and the proportions of cells expressing tropomyosin respectively were increased to a greater extent by TGF-beta1with cyclic strain than by TGF-beta1 alone. These results showed that cyclic strain promotes TGF-beta1mediated cardiomyogenic marker expression in BMMSCs in vitro.
Bhang
Suk Ho
SH
School of Chemical and Biological Engineering, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul 151-744, Republic of Korea.
Gwak
So-Jung
SJ
Lee
Tae-Jin
TJ
Kim
Sang-Soo
SS
Park
Ho Hyun
HH
Park
Moon Hyang
MH
Lee
Dae-Hee
DH
Lee
Soo-Hong
SH
Kim
Byung-Soo
BS
D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D005434:Flow Cytometry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033081:Shear Strength; D012825:Silicon / Q000737:chemistry; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*; D019210:Troponin I / Q000378:metabolism
D015415:Biomarkers; D015676:Osteonectin; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D019210:Troponin I; D015675:Osteocalcin; D018995:Myosin Heavy Chains; D012825:Silicon