Science letters: Proteomic analysis of differentially expressed proteins in mice with concanavalin A-induced hepatitis.
OBJECTIVE
To find new protein biomarkers for the detection and evaluation of liver injury and to analyze the relationship between such proteins and disease progression in concanavalin A (Con A)-induced hepatitis.


METHODS
Twenty-five mice were randomly divided into five groups: an untreated group, a control group injected with phosphate buffered saline (PBS), and groups with Con A-induced hepatitis evaluated at 1, 3 and 6 h. Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify differences in protein expression among groups. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the results.


RESULTS
In mice with Con A-induced hepatitis, expression levels of four proteins were increased: RIKEN, fructose bisphosphatase 1 (fbp1), ketohexokinase (khk), and Chain A of class pi glutathione S-transferase. Changes in fbp1 and khk were confirmed by qRT-PCR.


CONCLUSIONS
Levels of two proteins, fbp1 and khk, are clearly up-regulated in mice with Con A-induced hepatitis.
Tan
Xu-fei
XF
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
Chen
Feng
F
Wu
Shan-shan
SS
Shi
Yu
Y
Liu
Dong-cheng
DC
Chen
Zhi
Z
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology* / Q000378:metabolism*; D003208:Concanavalin A; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020543:Proteome / Q000032:analysis*
D015415:Biomarkers; D020543:Proteome; D003208:Concanavalin A