Molecular cloning of Sdr4, a regulator involved in seed dormancy and domestication of rice.
Seed dormancy provides a strategy for flowering plants to survive adverse natural conditions. It is also an important agronomic trait affecting grain yield, quality, and processing performance. We cloned a rice quantitative trait locus, Sdr4, which contributes substantially to differences in seed dormancy between japonica (Nipponbare) and indica (Kasalath) cultivars. Sdr4 expression is positively regulated by OsVP1, a global regulator of seed maturation, and in turn positively regulates potential regulators of seed dormancy and represses the expression of postgerminative genes, suggesting that Sdr4 acts as an intermediate regulator of dormancy in the seed maturation program. Japonica cultivars have only the Nipponbare allele (Sdr4-n), which endows reduced dormancy, whereas both the Kasalath allele (Srd4-k) and Sdr4-n are widely distributed in the indica group, indicating prevalent introgression. Srd4-k also is found in the wild ancestor Oryza rufipogon, whereas Sdr4-n appears to have been produced through at least two mutation events from the closest O. rufipogon allele among the accessions examined. These results are discussed with respect to possible selection of the allele during the domestication process.
Sugimoto
Kazuhiko
K
National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
Takeuchi
Yoshinobu
Y
Ebana
Kaworu
K
Miyao
Akio
A
Hirochika
Hirohiko
H
Hara
Naho
N
Ishiyama
Kanako
K
Kobayashi
Masatomo
M
Ban
Yoshinori
Y
Hattori
Tsukaho
T
Yano
Masahiro
M
D000483:Alleles; D001483:Base Sequence; D003001:Cloning, Molecular; D018556:Crops, Agricultural / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D017343:Genes, Plant; D005816:Genetic Complementation Test; D054643:INDEL Mutation; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D010802:Phylogeny; D030821:Plants, Genetically Modified; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D018749:RNA, Plant