Supplementation of ascorbic acid prevents oxidative damages in arsenic-loaded hepatic tissue of rat: an ex vivo study.
Oxidative stress due to arsenic toxicity and ameliorative potentiality of L-ascorbic acid was evaluated in an ex vivo system of rat hepatic tissue. The study revealed that arsenic increased the activity of superoxide dismutase (SOD) and catalase (CAT) and the level of lipid peroxidation (LPO), protein carbonyl (PC) and nitric oxide (NO) at 1 hour, 1.5 hours and 2 hours of incubation. Co-treatment with L-ascorbic acid was found effective to normalize the activity of SOD and CAT and the production of LPO, PC and NO in hepatic tissue. This ex vivo study suggested that ascorbic acid is helpful to ameliorate arsenic-induced oxidative stress. This may be one of the alternative screening systems to study the efficacy of antioxidant and hepatoprotective agent.
Rana
Tanmoy
T
Indian Veterinary Research Institute, Kolkata, India.
Bera
Asit Kumar
AK
Das
Subhashree
S
Pan
Diganta
D
Bandyopadhyay
Subhasish
S
Bhattacharya
Debasis
D
De
Sumanta
S
Das
Subrata Kumar
SK
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001151:Arsenic / Q000633:toxicity*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004785:Environmental Pollutants / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D009924:Organ Culture Techniques; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D018675:Toxicity Tests
D000975:Antioxidants; D004785:Environmental Pollutants; D009569:Nitric Oxide; D002374:Catalase; D013482:Superoxide Dismutase; D001151:Arsenic; D001205:Ascorbic Acid