title: Correlating lesion size and location to deficits after ischemic stroke: the influence of accounting for altered peri-necrotic tissue and incidental silent infarcts.
abstract: BACKGROUND
  Investigators frequently quantify and evaluate the location and size of stroke lesions to help uncover cerebral anatomical correlates of deficits observed after first-ever stroke. However, it is common to discover silent infarcts such as lacunes in patients identified clinically as 'first-ever' stroke, and it is unclear if including these incidental findings may impact lesion-based investigations of brain-behaviour relationships. There is also debate concerning how to best define the boundaries of necrotic stroke lesions that blend in an ill-defined way into surrounding tissue, as it is unclear whether including this altered peri-necrotic tissue region may influence studies of brain-behaviour relationships. Therefore, for patients with clinically overt stroke, we examined whether including altered peri-necrotic tissue and incidental silent strokes influenced either lesion volume correlations with a measure of sensorimotor impairment or the anatomical localization of this impairment established using subtraction lesion analysis.
  
  
  METHODS
  Chronic stroke lesions of 41 patients were manually traced from digital T1-MRI to sequentially include the: necrotic lesion core, altered peri-necrotic tissue, silent lesions in the same hemisphere as the index lesion, and silent lesions in the opposite hemisphere. Lesion volumes for each region were examined for correlation with motor impairment scores, and subtraction analysis was used to highlight anatomical lesion loci associated with this deficit.
  
  
  RESULTS
  For subtraction lesion analysis, including peri-necrotic tissue resulted in a larger region of more frequent damage being seen in the basal ganglia. For correlational analysis, only the volume of the lesion core was significantly associated with motor impairment scores (r = -0.35, p = 0.025). In a sub-analysis of patients with small subcortical index lesions, adding silent lesions in the opposite hemisphere to the volume of the index stroke strengthened the volume-impairment association.
  
  
  CONCLUSIONS
  Including peri-necrotic tissue strengthened lesion localization analysis, but the influence of peri-necrotic tissue and incidental lesions on lesion volume correlations with motor impairment was negligible barring a small index lesion. Overall, the potential influence of incidental lesions and peri-necrotic tissue on brain-behaviour relationships may depend on the characteristics of the index stroke and on whether one is examining the relationship between lesion volume and impairment or lesion location and impairment.
authors.affiliation: Heart and Stroke Foundation Centre for Stroke Recovery, ON, Canada.
authors.forename: Lisa D
authors.initials: LD
authors.lastname: Alexander
authors.forename: Sandra E
authors.initials: SE
authors.lastname: Black
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Gao
authors.forename: Gregory
authors.initials: G
authors.lastname: Szilagyi
authors.forename: Cynthia J
authors.initials: CJ
authors.lastname: Danells
authors.forename: William E
authors.initials: WE
authors.lastname: McIlroy
mesh_terms: D000368:Aged; D020520:Brain Infarction / Q000150:complications / Q000473:pathology*; D002545:Brain Ischemia / Q000150:complications / Q000473:pathology*; D002540:Cerebral Cortex / Q000473:pathology*; D003430:Cross-Sectional Studies; D016208:Databases, Factual; D005260:Female; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D019957:Motor Skills Disorders / Q000209:etiology / Q000473:pathology*; D009336:Necrosis; D020521:Stroke / Q000150:complications / Q000473:pathology*