Preparation and orcein staining of mitotic chromosomes from Drosophila larval brain.
In this protocol, larval brains from Drosophila are incubated in vitro with colchicine, treated with hypotonic solution, fixed, and squashed in aceto-orcein. This procedure provides a large number of well-spread metaphase figures (200-400 per brain) that can be analyzed for chromosome morphology, the presence of chromosome aberrations, and the degree of ploidy.
Pimpinelli
Sergio
S
Bonaccorsi
Silvia
S
Fanti
Laura
L
Gatti
Maurizio
M
D000818:Animals; D001921:Brain / Q000166:cytology; D002875:Chromosomes; D004330:Drosophila / Q000166:cytology*; D005456:Fluorescent Dyes; D007814:Larva / Q000166:cytology; D008938:Mitosis; D010078:Oxazines; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D010078:Oxazines; C002313:PAcein