title: [Simultaneous determination of three sesquiterpene lactones in Radix Linderae by HPLC].
abstract: OBJECTIVE
  To develop an HPLC method for simultaneous determination of three major sesquiterpene lactones in Radix Linderae.
  
  
  METHODS
  The chromatographic separation was achieved on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) using isocratic elution of acetonitrile-water (containing 0.1% H3 PO4) (45 : 55) at a flow rate of 1.0 mL x min(-1). Detection was carried out using a photodiode array detector at 220 nm.
  
  
  RESULTS
  The calibration curves were linear in the range of 0.001 8-0.036 0 g x L(-1) for hydroxylinderstrenolide (R2 = 0.999 8), 0.016 2-0.323 2 g x L(-1) for neolinderalactone (R2 = 0.999 9), 0.010 5-0.209 9 g x L(-1) for linderane (R2 = 0.999 9), respectively. The average recoveries were 100.0% for hydroxylinderstrenolide, 98.8% for neolinderalactone and 98.9% for linderane with RSD not more than 3.3%.
  
  
  CONCLUSIONS
  The established method was proved to be simple, sensitive and credible, and can be applied to quality control of Radix Linderae.
authors.affiliation: Institute of Modern Chinese Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Zheng
authors.forename: Lianjun
authors.initials: L
authors.lastname: Luan
authors.forename: Lishe
authors.initials: L
authors.lastname: Gan
authors.forename: Changxin
authors.initials: C
authors.lastname: Zhou
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Wu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D007783:Lactones / Q000032:analysis*; D027443:Lindera / Q000737:chemistry*; D012717:Sesquiterpenes / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D007783:Lactones; D012717:Sesquiterpenes