title: [Study on callus induction of Ferula sinkiangensis].
abstract: OBJECTIVE
  To explore the effect of different culture conditions and hormone combinations on callus induction of Ferula sinkiangensis.
  
  
  METHODS
  Using different seedling age of Ferula sinkiangensis young cotyledon, hypocotyl, radicle as explants, which were cultivated on differentiation medium with different hormone combinations, to study the effects on Ferulic callus by different explants and hormone combinations.
  
  
  RESULTS
  Ferulic seedlings with no true leaf was more favorable to the callus, the optimum medium for hypocotyl induction was MS + 2,4-D (1.0 mg/L) + KT (1.5 mg/L), for radicle induction was MS + NAA (0.5 mg/L) + 6-BA (0.5 mg/L), and the best subculture medium was MS + NAA (1.5 mg/L) + 6-BA (2.5 mg/L).
  
  
  CONCLUSIONS
  NAA,2,4-D and 6-BA must have the inductive effects, 6-BA is better than the KT in the process of proliferation and the medium containing GA3 is conducive to the process of subculture embryogenic callus production.
authors.affiliation: Xin Jiang Institute of Chinese Traditional Medical and Ethical Materia Medica, Urumqi 830002, China. zhujunlhao@163.com
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Xiao-jin
authors.initials: XJ
authors.lastname: Li
authors.forename: Sulaiman
authors.initials: S
authors.lastname: Kaisa
authors.forename: Xiao-guang
authors.initials: XG
authors.lastname: Jia
mesh_terms: D002681:China; D003470:Culture Media / Q000737:chemistry; D005309:Ferula / Q000254:growth & development* / Q000378:metabolism; D006728:Hormones / Q000378:metabolism / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods
chemical_list: D003470:Culture Media; D006728:Hormones; D010937:Plant Growth Regulators