AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis.
The calcium-dependent protein kinase (CDPK) family is needed in plant signaling during various physiological pathways. The Arabidopsis AtCPK6 gene belongs to the subclass of stress-inducible CDPKs, which is stimulated by salt and osmotic stress. To elucidate the physiological function of AtCPK6, transgenic Arabidopsis plants under the control of double CaMV 35S promoter were obtained. AtCPK6 over-expressing plants showed enhanced tolerance to salt/drought stresses. The elevated tolerance of the AtCPK6 over-expressing plants was confirmed by the change of proline and malondialdehyde (MDA). Real-time PCR analyses revealed that the expression levels of several stress-regulated genes were altered in AtCPK6 over-expressing plants. However, cpk6 mutant displayed no obvious difference with control. These results are likely to indicate that AtCPK6 is functionally redundant and a positive regulator involved in the tolerance to salt/drought stress in Arabidopsis.
Xu
Jing
J
Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, China.
Tian
Yong-Sheng
YS
Peng
Ri-He
RH
Xiong
Ai-Sheng
AS
Zhu
Bo
B
Jin
Xiao-Fen
XF
Gao
Feng
F
Fu
Xiao-Yan
XY
Hou
Xi-Lin
XL
Yao
Quan-Hong
QH
D000222:Adaptation, Physiological / Q000187:drug effects* / Q000235:genetics; D017360:Arabidopsis / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D008954:Models, Biological; D010802:Phylogeny; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics
D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D012333:RNA, Messenger; C036483:T-DNA; D012965:Sodium Chloride; C516787:CPK6 protein, Arabidopsis; D017871:Calcium-Calmodulin-Dependent Protein Kinases