title: Matrix metalloproteinase: investigation from gene to protein as effective factor in myocardial infarction.
abstract: Current evidence indicates that extracellular matrix (ECM) remodeling is a component of acute myocardial infarction (AMI) and matrix metalloproteinase (MMP) has a role in early atherosclerosis, plaque rupture and myocardial infarction (MI). The necessity of inhibition of ECM remodeling and subsequent injuries in patients with AMI suggests that MMP might be involved in this task. Therefore, we investigated the activities of MMP-1, -2, -3, and -9 which play an important role in AMI. Plasma and peripheral blood mononuclear cells (PBMCs) of 50 patients with AMI were isolated from peripheral blood after the onset of AMI within 24 h, comparing with 50 control subjects. The active form of MMPs was measured by enzyme linked immunosorbent assay (ELISA); MMP proteins presence and expression by immunoblotting and zymography analysis; and mRNA expression of MMPs by real time reverse transcriptase polymerase chain reaction. Plasma concentrations of MMPs increase in patients rather than control subjects. Gel zymography revealed 43, 66, 45, and 83 kDa molecular weight bands which consistent with active MMP-1, -2, -3, and -9, respectively, exhibiting gelatin-degrading activity in both patient and control subjects. No up-regulation of mRNA expression was found. To our knowledge, it is the first monitoring of MMP gene and protein expression and also circulating active MMPs in Iranian patients with AMI and normal subjects. Up-regulation of MMPs activity is common in the falling myocardium and missing up-regulation of transcription indicates that protein levels of MMPs were regulated at the post transcriptional level.
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran. sghaderian@yahoo.co.uk
authors.forename: Sayyed Mohammad Hossein
authors.initials: SM
authors.lastname: Ghaderian
authors.forename: Reza Akbarzadeh
authors.initials: RA
authors.lastname: Najar
authors.forename: Akram Sadat
authors.initials: AS
authors.lastname: Tabatabaei Panah
authors.forename: Gashin
authors.initials: G
authors.lastname: Rezaie
authors.forename: Azam
authors.initials: A
authors.lastname: Rezaei Farimani
authors.forename: Asghar
authors.initials: A
authors.lastname: Beigi Harchegani
authors.forename: Eznollah
authors.initials: E
authors.lastname: Azargashb
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005109:Extracellular Matrix / Q000201:enzymology / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000096:biosynthesis* / Q000235:genetics*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000201:enzymology* / Q000235:genetics*; D011446:Prospective Studies; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D020782:Matrix Metalloproteinases