A spectroscopic study of 2-[4'-(dimethylamino)phenyl]-benzothiazole binding to insulin amyloid fibrils.
The spectroscopic properties of 2-[4'-(dimethylamino)phenyl]-benzothiazole (BTA-2) in solution and in the presence of amyloid fibrils were investigated using absorption and fluorescence spectroscopy. Solution studies show that BTA-2 forms micelles in aqueous solutions, but that the dye can be solvated upon the addition of acetonitrile (CH(3)CN). BTA-2 binds to amyloid fibrils in solution leading to a characteristic blue-shift in the emission spectrum and an increase in fluorescence intensity. However, in solutions with increasing CH(3)CN concentration, there was a marked decrease in binding of the BTA-2 to fibrils. Studies demonstrating the effect of BTA-2 concentration on binding were performed. A comparison with the standard amyloid fluorescent marker, thioflavin T (ThT), showed that BTA-2 is more fluorescent, making it an excellent dye to label amyloid samples.
Kitts
Catherine C
CC
Department of Chemistry and Biochemistry, Center for Nano and Molecular Science and Technology, and Texas Materials Institute, University of Texas at Austin, Austin, TX 78712, USA.
Vanden Bout
David Anton
DA
D000042:Absorption; D000682:Amyloid / Q000378:metabolism*; D000814:Aniline Compounds / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry* / Q000378:metabolism*; D002417:Cattle; D005454:Fluorescence Polarization; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000378:metabolism*; D011485:Protein Binding; D012996:Solutions; D012997:Solvents / Q000737:chemistry; D013057:Spectrum Analysis
C554933:2-(4'-(dimethylamino)phenyl)benzothiazole; D000682:Amyloid; D000814:Aniline Compounds; D052160:Benzothiazoles; D007328:Insulin; D012996:Solutions; D012997:Solvents; C005465:benzothiazole