title: Chemically ubiquitylated PCNA as a probe for eukaryotic translesion DNA synthesis.
abstract: The rapid growth in ubiquitin biology requires facile chemical approaches for protein ubiquitylation that can overcome the common problem of low yield faced by the enzymatic reaction catalyzed by ubiquitin ligases. We report a chemical approach for monoubiquitylation and SUMOylation of PCNA through disulfide exchange and intein chemistry. We used the chemically ubiquitylated and SUMOylated PCNAs in studying translesion DNA synthesis and revealed a surprising degree of flexibility of the ubiquitin modification.
authors.affiliation: Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware, USA.
authors.forename: Junjun
authors.initials: J
authors.lastname: Chen
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Ai
authors.forename: Jialiang
authors.initials: J
authors.lastname: Wang
authors.forename: Lajos
authors.initials: L
authors.lastname: Haracska
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Zhuang
mesh_terms: D003545:Cysteine / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D005057:Eukaryotic Cells / Q000378:metabolism*; D047668:Inteins; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D025801:Ubiquitin / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism; D057149:Ubiquitinated Proteins / Q000737:chemistry / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D004220:Disulfides; D015335:Molecular Probes; D018809:Proliferating Cell Nuclear Antigen; D025801:Ubiquitin; D057149:Ubiquitinated Proteins; D004247:DNA; D044767:Ubiquitin-Protein Ligases; D004259:DNA-Directed DNA Polymerase; D003545:Cysteine