title: Assessment of Protein Stability in Cerebrospinal Fluid Using Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Protein Profiling.
abstract: Recent studies have evaluated proper acquisition and storage procedures for the use of serum or plasma for mass spectrometry (MS)-based proteomics. The present study examines the proteome stability of human cerebrospinal fluid (CSF) over time at 23°C (room temperature) and 4°C using surface-enhanced laser desorption/ionization time-of-flight MS. Data analysis revealed that statistically significant differences in protein profiles are apparent within 4 h at 23°C and between 6 and 8 h at 4°C. Inclusion of protease and phosphatase inhibitor cocktails into the CSF samples failed to significantly reduce proteome alterations over time. We conclude that MS-based proteomic analysis of CSF requires careful assessment of sample collection procedures for rapid and optimal sample acquisition and storage.
authors.affiliation: Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA.
authors.forename: Srikanth
authors.initials: S
authors.lastname: Ranganathan
authors.forename: Anna
authors.initials: A
authors.lastname: Polshyna
authors.forename: Georgina
authors.initials: G
authors.lastname: Nicholl
authors.forename: James
authors.initials: J
authors.lastname: Lyons-Weiler
authors.forename: Robert
authors.initials: R
authors.lastname: Bowser