Influence of the BGE composition on analyte response in CD-mediated NACE-MS.
The influence of the BGE composition, including the addition of a single-isomer sulfated beta-CD derivative, on the ionization performance of the model compound carvedilol in NACE-ESI-MS was studied using an alternative infusion method. This approach employs voltage-induced infusion of the BGE containing the analyte, and takes into account the effects of variations in EOF and effective analyte mobility on the ESI-MS intensity. First, the optimal composition of the sheath liquid for CE-MS in terms of signal abundance and stability was determined. The BGE ammonium formate, acetate, and camphorsulfonate were found to have similar effects on analyte ionization. Addition of single-isomer sulfated beta-CD derivatives (available as sodium salt) to the BGE revealed that the anionic CD derivatives did not give rise to the same ionization suppression effect. This result can be attributed to differences in the dissociation state of these sodium salts. Finally, it is shown that information about chiral selectivity can also be obtained with the applied infusion method.
Servais
Anne-Catherine
AC
Dept. of Analytical Pharmaceutical Chemistry, Institute of Pharmacy, University of Liège, Liège, Belgium.
Fillet
Marianne
M
Dept. of Analytical Pharmaceutical Chemistry, Institute of Pharmacy, University of Liège, Liège, Belgium.
Mol
Roelof
R
Dept. of Biomedical Analysis, Utrecht University, Utrecht, The Netherlands.
Rousseau
Anne
A
Dept. of Analytical Pharmaceutical Chemistry, Institute of Pharmacy, University of Liège, Liège, Belgium.
Crommen
Jacques
J
Dept. of Analytical Pharmaceutical Chemistry, Institute of Pharmacy, University of Liège, Liège, Belgium.
Somsen
Govert W
GW
Dept. of Biomedical Analysis, Utrecht University, Utrecht, The Netherlands.
de Jong
Gerhardus J
GJ
Dept. of Biomedical Analysis, Utrecht University, Utrecht, The Netherlands.
D000838:Anions; D002227:Carbazoles / Q000032:analysis; D000077261:Carvedilol; D004573:Electrolytes / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D011412:Propanolamines / Q000032:analysis; D013237:Stereoisomerism; D013431:Sulfates / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000838:Anions; D002227:Carbazoles; D004573:Electrolytes; D011412:Propanolamines; D013431:Sulfates; D047392:beta-Cyclodextrins; D000077261:Carvedilol