title: The rate of unequal crossing over in the dumpy gene from Drosophila melanogaster.
abstract: The PIGSFEAST (PF) exon of the Drosophila dumpy gene is undergoing concerted evolution by the process of unequal crossing over. We have developed a long-range PCR-based assay to amplify the approximately 12 kb long exon which contains variable numbers of 303 or 306 nt long repeats in a tandem array. We applied this procedure to mutation accumulation lines of Drosophila melanogaster established by M. Wayne and L. Higgins. Nine new repeat length variants were found in these lines allowing us to measure the rate of unequal crossing over in the PF exon. The rate, which for several reasons is an underestimate, is 7.05 x 10(-4) exchanges per generation.
authors.affiliation: Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.
authors.forename: Amber
authors.initials: A
authors.lastname: Carmon
authors.forename: Matthew
authors.initials: M
authors.lastname: Larson
authors.forename: Marta
authors.initials: M
authors.lastname: Wayne
authors.forename: Ross
authors.initials: R
authors.lastname: MacIntyre
mesh_terms: D000818:Animals; D003434:Crossing Over, Genetic / Q000235:genetics* / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D005787:Gene Frequency; D008954:Models, Biological; D009154:Mutation / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
chemical_list: C411950:DPY protein, Drosophila; D029721:Drosophila Proteins; D016326:Extracellular Matrix Proteins