title: Minute negative superhelicity is sufficient to induce the B-Z transition in the presence of low tension.
abstract: Left-handed Z-DNA has fascinated biological scientists for decades by its extraordinary structure and potential involvement in biological phenomena. Despite its instability relative to B-DNA, Z-DNA is stabilized in vivo by negative supercoiling. A detailed understanding of Z-DNA formation is, however, still lacking. In this study, we have examined the B-Z transition in a short guanine/cytosine (GC) repeat in the presence of controlled tension and superhelicity via a hybrid technique of single-molecule FRET and magnetic tweezers. The hybrid scheme enabled us to identify the states of the specific GC region under mechanical control and trace conformational changes synchronously at local and global scales. Intriguingly, minute negative superhelicity can facilitate the B-Z transition at low tension, indicating that tension, as well as torsion, plays a pivotal role in the transition. Dynamic interconversions between the states at elevated temperatures yielded thermodynamic and kinetic constants of the transition. Our single-molecule studies shed light on the understanding of Z-DNA formation by highlighting the highly cooperative and dynamic nature of the B-Z transition.
authors.affiliation: Department of Physics, Korea University, Seoul 136-713, South Korea.
authors.forename: Mina
authors.initials: M
authors.lastname: Lee
authors.forename: Sook Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Seok-Cheol
authors.initials: SC
authors.lastname: Hong
mesh_terms: D001482:Base Composition; D001696:Biomechanical Phenomena; D043542:DNA, Z-Form / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D007700:Kinetics; D009690:Nucleic Acid Conformation; D013696:Temperature; D013997:Time Factors; D054159:Torsion, Mechanical
chemical_list: D043542:DNA, Z-Form