title: [Effects of allergen and intranasal glucocorticoid on Th17 and RORgamma t in peripheral blood in patients with allergic rhinitis.].
abstract: OBJECTIVE
  To study the effect of specific immunotherapy and intranasal glucocorticoid on T help 17 (Th17) cells and RORgammat in peripheral blood in patients with allergic rhinitis (AR).
  
  
  METHODS
  Forty patients with allergic rhinitis (group A) were divided randomly into two subgroups (group A1 and A2), and each subgroup had 20 patients. The patients in group A1 were treated with intranasal glucocorticoid (INGS) for one-year. The patients in group A2 were treated with special immunotherapy (SIT) for 4 weeks. Blood samples were respectively taken from 10 healthy individuals (group B), 20 AR patients (group A1) before and after SIT with specific standardized allergen and 20 AR patients (group A2) before and after INGS. The ratio of Th17 cells in peripheral blood monouclear cells (PBMC) were analysed by flow cytometry. The expression of RORgammat mRNA were detected by real-time polymerase chain reaction and the interleukin-23(IL-23), IL-17, IL-6 were detected by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The ratio of Th17 cells in PBMC and the expression of RORgammat mRNA in group A [(18.97 +/- 1.05)% and (0.604 +/- 0.027)] were respectively higher than those in group B [(15.12 +/- 1.09)% and (0.447 +/- 0.024)] and the difference reached statistical significance (t were respectively -10.056 and -17.986, each P < 0.01). The level of IL-6, IL-17 and IL-23 in group A were respectively higher than those in group B and the difference reached statistical significance (t were respectively -41.149, -17.618 and -26.824, all P < 0.01). The ratio of Th17 cells in PBMC, the expression of RORgammat mRNA, the level of IL-6, IL-17 and IL-23 before INGS did not show significant difference from those of after INGS in group A1 (t were respectively 0.298, 0.240, -1.136, 0.283 and -1.670, all P > 0.05). The ratio of Th17 cells in PBMC and the expression of RORgammat mRNA were respectively (18.99 +/- 1.14)% and (0.603 +/- 0.027) before SIT and were respectively (16.30 +/- 1.63)% and (0.429 +/- 0.023) after SIT in group A2, and the difference reached statistical significance (t were respectively 6.035 and 22.015, all P < 0.01). The level of IL-6, IL-17 and IL-23 before SIT were lower respectively than those of after SIT in group A2 and the difference reached statistical significance (t were respectively 9.235, 11.289, 7.267, all P < 0.01).
  
  
  CONCLUSIONS
  The ratio of Th17 cells in PBMC, the expression of RORgammat mRNA, the level of IL-6, IL-17 and IL-23 were up-regulated in patients with AR. The treatment of SIT could get the 5 items down and the treatment of INGS couldn't.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, the People's Hospital of Guangxi Zhuang Autonmous Region, Nanning 530021, China.
authors.forename: Shen-Hong
authors.initials: SH
authors.lastname: Qu
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Huang
authors.forename: Zhi-Ying
authors.initials: ZY
authors.lastname: Ou
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Lin
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Liang
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Lu
mesh_terms: D000485:Allergens / Q000008:administration & dosage; D006801:Humans; D020381:Interleukin-17; D007963:Leukocytes, Mononuclear; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D065631:Rhinitis, Allergic
chemical_list: D000485:Allergens; D020381:Interleukin-17; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3