Influence of human cytomegalovirus infection on the NK cell receptor repertoire in children.
Human cytomegalovirus (hCMV) infection is usually asymptomatic but may cause disease in immunocompromised hosts. It has been reported that hCMV infection may shape the NK cell receptor (NKR) repertoire in adult individuals, promoting a variable expansion of the CD94/NKG2C+ NK cell subset. We explored the possible relationship between this viral infection and the expression pattern of different NKR including CD94/NKG2C, CD94/NKG2A, immunoglobulin-like transcript 2 (ILT2, CD85j), KIR2DL1/2DS1, KIR3DL1, and CD161 in peripheral blood lymphocytes from healthy children, seropositive (n=21) and seronegative (n=20) for hCMV. Consistent with previous observations in adults, a positive serology for hCMV was associated with increased numbers of NKG2C+ NK and T cells as well as with ILT2+ T lymphocytes. Moreover, the proportions of CD161+ and NKG2C+CD56-CD3- NK cells also tended to be increased in hCMV+ individuals. Excretion of the virus was associated with higher proportions of NKG2C+ NK cells. Altogether, these data reveal that hCMV may have a profound influence on the NKR repertoire in early childhood.
Monsiváis-Urenda
Adriana
A
Department of Immunology, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico.
Noyola-Cherpitel
Daniel
D
Hernández-Salinas
Alba
A
García-Sepúlveda
Christian
C
Romo
Neus
N
Baranda
Lourdes
L
López-Botet
Miguel
M
González-Amaro
Roberto
R
D000914:Antibodies, Viral / Q000097:blood; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000097:blood / Q000276:immunology* / Q000652:urine; D005260:Female; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007223:Infant; D007694:Killer Cells, Natural / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D008297:Male; D055651:NK Cell Lectin-Like Receptor Subfamily B / Q000096:biosynthesis / Q000235:genetics; D055654:NK Cell Lectin-Like Receptor Subfamily C / Q000096:biosynthesis / Q000235:genetics; D051920:NK Cell Lectin-Like Receptor Subfamily D / Q000096:biosynthesis / Q000235:genetics; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics; D055607:Receptors, Natural Killer Cell / Q000096:biosynthesis* / Q000235:genetics; D012463:Saliva / Q000821:virology; D014556:Urine / Q000821:virology
D000914:Antibodies, Viral; D015703:Antigens, CD; D007074:Immunoglobulin G; C527203:KLRB1 protein, human; C527059:KLRC2 protein, human; C497869:KLRD1 protein, human; C105299:LILRB1 protein, human; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D055651:NK Cell Lectin-Like Receptor Subfamily B; D055654:NK Cell Lectin-Like Receptor Subfamily C; D051920:NK Cell Lectin-Like Receptor Subfamily D; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell