title: Detection of vesicant-induced upper airway mucosa damage in the hamster cheek pouch model using optical coherence tomography.
abstract: Hamster cheek pouches were exposed to 2-chloroethyl ethyl sulfide [CEES, half-mustard gas (HMG)] at a concentration of 0.4, 2.0, or 5.0 mg/ml for 1 or 5 min. Twenty-four hours post-HMG exposure, tissue damage was assessed by both stereomicrography and optical coherence tomography (OCT). Damage that was not visible on gross visual examination was apparent in the OCT images. Tissue changes were found to be dependent on both HMG concentration and exposure time. The submucosal and muscle layers of the cheek pouch tissue showed the greatest amount of structural alteration. Routine light microscope histology was performed to confirm the OCT observations.
authors.affiliation: University of California, Beckman Laser Institute and Medical Clinic, Irvine, California 92612, USA.
authors.forename: Marie J
authors.initials: MJ
authors.lastname: Hammer-Wilson
authors.forename: Vi
authors.initials: V
authors.lastname: Nguyen
authors.forename: Woong-Gyu
authors.initials: WG
authors.lastname: Jung
authors.forename: Yehchen
authors.initials: Y
authors.lastname: Ahn
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Chen
authors.forename: Petra
authors.initials: P
authors.lastname: Wilder-Smith
mesh_terms: D000818:Animals; D002610:Cheek / Q000293:injuries / Q000473:pathology; D002619:Chemical Warfare Agents / Q000633:toxicity; D006224:Cricetinae; D007509:Irritants / Q000633:toxicity*; D008297:Male; D008647:Mesocricetus; D008853:Microscopy; D009061:Mouth Mucosa / Q000187:drug effects* / Q000293:injuries / Q000473:pathology*; D009151:Mustard Gas / Q000031:analogs & derivatives* / Q000633:toxicity; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D002619:Chemical Warfare Agents; D007509:Irritants; C031278:2-chloroethyl ethyl sulfide; D009151:Mustard Gas