Production optimization and properties of beta glucosidases from a marine fungus Aspergillus-SA 58.
Aspergillus strain SA 58, showing considerable beta glucosidase production was selected as the potential strain. The fungus showed enzyme production in both acidic and alkaline pH. A temperature of 35 degrees C was found to be optimum for enzyme production. Maximum enzyme production was seen when pectin was used as the carbon source (80 U/ml). In solid-state fermentation, an enzyme production of 6200 U/g Initial Dry Substrate was noted. The strain produced two extra cellular enzymes and two intra cellular enzymes. For both the extra cellular enzymes (BGL A and BGL B), 60 degrees C was found to be optimum temperature for activity. BGL A showed an optimum pH of 4.0 while BGL B showed an optimum pH of 3.0 for activity. Both the enzymes showed a second peak of activity at pH 9.0. Both BGL A and BGL B showed high thermal stabilities with residual activities of 86% and 85% even after 6h of incubation at 50 degrees C.
Elyas
K K
KK
Immuno Technology Laboratory, Department of Biotechnology, Cochin, University of Science and Technology, Cochin 682022, India.
Mathew
Abraham
A
Sukumaran
Rajeev K
RK
Ali
P P Manzur
PP
Sapna
K
K
Kumar
S Ramesh
SR
Mol
K R Rekha
KR
D001230:Aspergillus / Q000187:drug effects / Q000201:enzymology*; D002244:Carbon / Q000494:pharmacology; D044602:Cellulases / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability / Q000187:drug effects; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D009584:Nitrogen / Q000494:pharmacology; D010368:Pectins / Q000494:pharmacology; D012623:Seawater / Q000382:microbiology*; D013696:Temperature
D002244:Carbon; D010368:Pectins; D044602:Cellulases; D009584:Nitrogen