Interaction between serotonin 5-HT2A receptor gene and dopamine transporter (DAT1) gene polymorphisms influences personality trait of persistence in Austrian Caucasians.
We examined 89 normal volunteers using Cloninger's Temperament and Character Inventory (TCI). Genotyping the 102T/C polymorphism of the serotonin 5HT2A receptor gene and the ser9gly polymorphism in exon 1 of the dopamine D3 receptor (DRD3) gene was performed using PCR-RFLP, whereas the dopamine transporter (DAT1) gene variable number of tandem repeats (VNTR) polymorphism was investigated using PCR amplification followed by electrophoresis in an 8% acrylamide gel with a set of size markers. We found a nominally significant association between gender and harm avoidance (P=0.017; women showing higher scores). There was no association of either DAT1, DRD3 or 5HT2A alleles or genotypes with any dimension of the TCI applying Kruskal-Wallis rank-sum tests. Comparing homozygote and heterozygote DAT1 genotypes, we found higher novelty seeking scores in homozygotes (P=0.054). We further found a nominally significant interaction between DAT1 and 5HT2A homo-/heterozygous gene variants (P=0.0071; DAT1 and 5HT2A genotypes P value of 0.05), performing multivariate analysis of variance (MANOVA). Examining the temperamental TCI subscales, this interaction was associated with persistence (genotypes: P=0.004; homo-/heterozygous gene variants: P=0.0004). We conclude that an interaction between DAT1 and 5HT2A genes might influence the temperamental personality trait persistence.
Schosser
Alexandra
A
Division of Biological Psychiatry, Department of Psychiatry & Psychotherapy, Medical University Vienna, Vienna, Austria. alexandra.schosser@meduniwien.ac.at
Fuchs
Karoline
K
Scharl
Theresa
T
Schloegelhofer
Monika
M
Kindler
Jochen
J
Mossaheb
Nilufar
N
Kaufmann
Rainer M
RM
Leisch
Friedrich
F
Kasper
Siegfried
S
Sieghart
Werner
W
Aschauer
Harald N
HN
D000328:Adult; D000483:Alleles; D001317:Austria; D050483:Dopamine Plasma Membrane Transport Proteins / Q000235:genetics*; D005106:Exploratory Behavior; D005260:Female; D005838:Genotype; D040261:Harm Reduction; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics; D010551:Personality / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D044402:Receptor, Serotonin, 5-HT2A / Q000235:genetics*; D050637:Receptors, Dopamine D3 / Q000235:genetics; D012737:Sex Factors; D018709:Statistics, Nonparametric; D044465:White People / Q000235:genetics* / Q000523:psychology
C493593:DRD3 protein, human; D050483:Dopamine Plasma Membrane Transport Proteins; D044402:Receptor, Serotonin, 5-HT2A; D050637:Receptors, Dopamine D3; C493214:SLC6A3 protein, human