Evidence that Vibrio vulnificus ahpC2 is essential for survival under high salinity by modulating intracellular level of ROS.
Expression of ahpC2 encoding an alkyl hydroperoxide reductase of Vibrio vulnificus, a foodborne pathogen, was incrementally induced depending on NaCl concentrations in the culture. Growth of the ahpC2 mutant was significantly impaired with longer lag phase and lower growth rate when cultured under high salinity. ROS was accumulated in V. vulnificus cells when stressed by exposure to high salinity, and the ahpC2 mutant accumulated higher level of ROS as compared with the parental wild type. Consequently, the combined results suggest that AhpC2 contributes to the growth of V. vulnificus under high salinity by scavenging ROS in cells.
Koh
Myung Jin
MJ
Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Lee
Hyun Sung
HS
Rhee
Jee Eun
JE
Choi
Sang Ho
SH
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism; D007424:Intracellular Fluid / Q000737:chemistry; D009154:Mutation; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D054712:Salinity; D012965:Sodium Chloride / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D041261:Vibrio vulnificus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D001426:Bacterial Proteins; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D012965:Sodium Chloride; D054464:Peroxiredoxins