title: Analysis of peptides using N-methylpolyvinylpyridium as silica surface modifier for CE-ESI-MS.
abstract: In this study, the N-methylpolyvinylpyridinuim polymer has for the first time been used as a silica surface modifier for CE in combination with ESI MS (CE-ESI-MS). The compatibility for ESI-MS was demonstrated by the analysis of peptides and protein digests. The N-methylpolyvinylpyridium surface interacts electrostatically with the ionized silanol groups, giving a cationic surface with a reversed EOF. The surface modifier gave rapid and repeatable separations of peptides, proteins and protein digests at acidic pH for more than 4 h of continuous use. The CE separation yielded peak efficiencies of up to 4.3 x 10(5) plates/m. The surface coating is highly compatible with ESI and facilitates the separation and analysis of complex peptide mixtures as shown by the analysis of BSA digests.
authors.affiliation: Analytical Chemistry, Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.
authors.forename: Anisa
authors.initials: A
authors.lastname: Elhamili
authors.affiliation: Analytical Chemistry, Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.
authors.forename: Magnus
authors.initials: M
authors.lastname: Wetterhall
authors.affiliation: Analytical Chemistry, Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.
authors.forename: Marcus
authors.initials: M
authors.lastname: Sjödin
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Polytechnic of Milano, Milano, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Sebastiano
authors.affiliation: Analytical Chemistry, Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Bergquist
mesh_terms: D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: C550523:N-methylpolyvinylpyridinium; D010455:Peptides; D011145:Polyvinyls; D011726:Pyridinium Compounds; D012822:Silicon Dioxide