title: The Bloom syndrome protein limits the lethality associated with RAD51 deficiency.
abstract: Little is known about the functional interaction between the Bloom's syndrome protein (BLM) and the recombinase RAD51 within cells. Using RNA interference technology, we provide the first demonstration that RAD51 acts upstream from BLM to prevent anaphase bridge formation. RAD51 downregulation was associated with an increase in the frequency of BLM-positive anaphase bridges, but not of BLM-associated ultrafine bridges. Time-lapse live microscopy analysis of anaphase bridge cells revealed that BLM promoted cell survival in the absence of Rad51. Our results directly implicate BLM in limiting the lethality associated with RAD51 deficiency through the processing of anaphase bridges resulting from the RAD51 defect. These findings provide insight into the molecular basis of some cancers possibly associated with variants of the RAD51 gene family.
authors.affiliation: Institut Curie, Centre de Recherche, Centre Universitaire, Orsay, France.
authors.forename: Kenza
authors.initials: K
authors.lastname: Lahkim Bennani-Belhaj
authors.forename: Sébastien
authors.initials: S
authors.lastname: Rouzeau
authors.forename: Géraldine
authors.initials: G
authors.lastname: Buhagiar-Labarchède
authors.forename: Pauline
authors.initials: P
authors.lastname: Chabosseau
authors.forename: Rosine
authors.initials: R
authors.lastname: Onclercq-Delic
authors.forename: Emilie
authors.initials: E
authors.lastname: Bayart
authors.forename: Fabrice
authors.initials: F
authors.lastname: Cordelières
authors.forename: Jérôme
authors.initials: J
authors.lastname: Couturier
authors.forename: Mounira
authors.initials: M
authors.lastname: Amor-Guéret
mesh_terms: D000705:Anaphase / Q000235:genetics; D016923:Cell Death / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference / Q000502:physiology; D051135:Rad51 Recombinase / Q000235:genetics*; D053484:RecQ Helicases / Q000235:genetics* / Q000378:metabolism; D012854:Sister Chromatid Exchange / Q000235:genetics
chemical_list: D051135:Rad51 Recombinase; C097151:Bloom syndrome protein; D053484:RecQ Helicases