title: Modification of titanium alloy surfaces for percutaneous implants by covalently attaching laminin.
abstract: Percutaneous implants require a seal at the skin interface. Laminin (L-332) is a component of the basement membrane, integral to epidermal attachment. To enhance the attachment of keratinocytes onto the surface of titanium alloy (Ti(6)Al(4)V), we attached L-332 onto the surface using silanization (L-332==Ti(6)Al(4)V). Iodinated L-332 was used to investigate protein attachment kinetics. L-332==Ti(6)Al(4)V remained attached after immersion in serum compared with adsorbed L-332. Cells from a keratinocyte line (HaCaT) grown on L-332==Ti(6)Al(4)V were significantly smaller (p < 0.05) with over a 20-fold increase in the number of adhesion plaques compared with nontreated Ti(6)Al(4)V control discs or with adsorbed L-332 surfaces. We have shown that it is possible to augment the surface of Ti(6)Al(4)V with L-332 and that this significantly increases the attachment of keratinocytes.
authors.affiliation: The Centre for Biomedical Engineering, Institute of Orthopaedics and Musculoskeletal Science, University College London, The Royal National Orthopaedic Hospital, Brockley Hill, Stanmore, Middlesex HA7 4LP, United Kingdom.
authors.forename: David J
authors.initials: DJ
authors.lastname: Gordon
authors.forename: Dimpu D
authors.initials: DD
authors.lastname: Bhagawati
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Pendegrass
authors.forename: Claire A
authors.initials: CA
authors.lastname: Middleton
authors.forename: Gordon W
authors.initials: GW
authors.lastname: Blunn
mesh_terms: D000497:Alloys / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D007797:Laminin / Q000737:chemistry* / Q000378:metabolism; D008422:Materials Testing; D019736:Prostheses and Implants; D011506:Proteins / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D016596:Vinculin / Q000378:metabolism
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D007797:Laminin; D011506:Proteins; D016596:Vinculin; D014025:Titanium