[Fabrication of titanium dioxide nanotube array and effects of its osteoblast proliferation and alkaline phosphatase activity].
OBJECTIVE
To investigate preparation parameters of TiO2 nanotube layer with anodization, and to evaluate the osteoblast activity on TiO2 nanotube layer in vitro.


METHODS
Titanium dioxide nanotube layer was grown using anodization method. TiO2 nanotube layers with different structure were obtained by controlling the voltage and time of anodization and rinsing process after anodization. Pure titanium without anodization was used as control. Osteoblasts were cultivated on the anodizated TiO2 nanotube, and the growth of osteoblasts was then evaluated and analyzed.


RESULTS
The voltage, duration of anodization and rinsing process following anodization were key factors to affect morphology of TiO2 nanotube layer. The area of osteoblast cultured on TiO2 nanotube layer was larger than that on pure titanium. Results from methylthiazol tetrazolium (MTT) test showed that the proliferation of osteoblasts after 96 h cultivation on TiO2 nanotube layer (0.62 +/- 0.02) was significantly higher than that on unanodizated titanium (0.55 +/- 0.03, P < 0.05). Three weeks later, the alkaline phosphatase (ALP) activity on TiO2 nanotube layer [(130.8 +/- 5.1) A(405)/mg] was significantly higher than that on unanodizated titanium [(109.6 +/- 4.5) A(405)/mg, P < 0.05].


CONCLUSIONS
The structure of TiO2 nanotube layer was greatly associated with anodization voltage. The TiO2 nanotube layer had a positive effect on osteoblast behavior.
Yu
Wei-qiang
WQ
Department of Prosthodontics, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Jiang
Xin-quan
XQ
Zhang
Yi-lin
YL
Zhang
Fu-qiang
FQ
D000469:Alkaline Phosphatase; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D043942:Nanotubes / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D014025:Titanium / Q000737:chemistry
C009495:titanium dioxide; D014025:Titanium; D000469:Alkaline Phosphatase