Knockdown of Li-cadherin increases metastatic behaviors of LoVo cells.
OBJECTIVE
The aim of this study was to investigate the role of Li-cadherin in invasion and metastasis in LoVo cells.


METHODS
We applied RNA interference mediated downregulation of Li-cadherin expression in LoVo cells. Li-cadherin expression in LoVo cells was examined by semiquantitative polymerase chain reaction, immunofluorescence, western blot, and immunoprecipitation, respectively. Effect of suppression of Li-cadherin expression on cell migration, invasion, and adhesion was detected by wound healing assay, migration assay, invasion assay, and adhesion assay. Expression and activity of MMP-2 and MMP-9 were analyzed by gelatin zymography.


RESULTS
Cell migration, invasion, and adhesion were increased concomitantly with the reduction in Li-cadherin protein expression. Furthermore, downregulation of Li-cadherin expression induced secretion of proMMP-9, active MMP-9 and active MMP-2.


CONCLUSIONS
This study suggests that silencing Li-cadherin has positive actions in the processes of LoVo cells invasion and metastasis, and the interactions among MMP-2, MMP-9, and Li-cadherin participate in the multiple steps of invasion and metastasis in LoVo colorectal cancer cells.
Yu
Qiong-Fang
QF
Department of Gastroenterology, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
Dong
Wei-Guo
WG
Ren
Jian-Lin
JL
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D001483:Base Sequence; D015820:Cadherins / Q000235:genetics*; D002448:Cell Adhesion; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology; D017931:DNA Primers; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D009362:Neoplasm Metastasis / Q000235:genetics* / Q000473:pathology; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012334:RNA, Neoplasm / Q000235:genetics; D014162:Transfection; D014945:Wound Healing
C490584:CDH17 protein, human; D015820:Cadherins; D017931:DNA Primers; D012334:RNA, Neoplasm