[Simultaneous determination of two anthraquinone-O-glucosides in five processed pieces from Rheum palamatum].
OBJECTIVE
To compare the contents of two anthraquinone-O-glucosides in five different processed products from Rheum palamatum.


METHODS
The content of aleo-emodin-8-O-beta-D-glucoside, rhein-8-O-beta-D-glucoside were determined simultaneously by HPLC on a agilent TC-C18 (2) column at 35 degrees C with the mobile phase of acetonitrile-1% glacial acetic acid (20: 80). The detection wavelength was set at 410 nm and the flow rate was 1.0 mL x min(-1).


RESULTS
The calibration curves for two components were linear within the range of 0.017 44-0.348 8 microg (r = 0.999 8, n = 5), 0.084-1.686 microg (r = 0.999 9, n = 5), and the average recoveries were 97.82%, 97.87%, respectively.


CONCLUSIONS
The results showed that there were remarkable variation of the two kinds of glucosides contentsamong the five different processed pieces. The contents of the crude pieces, pieces roasted with millet wine and pieces roasted with vinegar were almost the same, while the prepared pieces and charring pieces had evidently reduced contents. The are certain regularity in the variation.
Zhang
Cun
C
Institute of Chinese Materia Madica, China Academy of Chinese Medicial Sciences, Beijing 100700, China.
Li
Li
L
Xiao
Yongqing
Y
Lin
Na
N
Liu
Chunfang
C
Tian
Guofang
G
Chen
Dongdong
D
D000880:Anthraquinones / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry*; D012250:Rheum / Q000737:chemistry*
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D005960:Glucosides