title: Investigation on the effects of ciclosporin (Atopica) on intradermal test reactivity and allergen-specific immunoglobulin (IgE) serology in atopic dogs.
abstract: The ability to use ciclosporin (Atopica®: Novartis Animal Health, Greensboro, NC, USA) prior to intradermal testing (IDT) would help avoid exacerbation of clinical disease that can be associated with drug withdrawal. This study evaluated the effects of 30 days of administration of ciclosporin at a dose of 5 mg/kg once daily on IDT reactivity (immediate phase reactions) in a group of dogs with atopic dermatitis (AD) with initial positive IDT reactions. 16 dogs diagnosed with AD were included in the study. Eight dogs (group A) were treated with ciclosporin orally at 5 mg/kg once daily for 30 days. Eight dogs (group P) were treated with a placebo orally once daily for 30 days. IDT was performed at day 0 and day 30 on all dogs enrolled using a standardized panel of 45 aqueous allergens (Greer Laboratories, Lenoir, NC, USA) appropriate to our geographical region. IDT reactivity was assessed by both subjective and objective methods at 15 min post-intradermal injection. Serum for allergen-specific immunoglobulin (IgE) serology was obtained at day 0 and day 30. The study was designed as a double-blinded, placebo-controlled, cross-over study. Data were analysed using a split-plot analysis of variance with the grouping factor of treatment and the repeat factor of time (SAS System for Windows). At week 4, ciclosporin did not have a statistically significant effect on IDT reactivity or serology results. It therefore appears that, no withdrawal is recommended to evaluate immediate phase reactions.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA. goldma44@cvm.msu.edu
authors.forename: Candice
authors.initials: C
authors.lastname: Goldman
authors.forename: Edmund
authors.initials: E
authors.lastname: Rosser
authors.forename: Annette
authors.initials: A
authors.lastname: Petersen
authors.forename: Joe
authors.initials: J
authors.lastname: Hauptman
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D018592:Cross-Over Studies; D016572:Cyclosporine / Q000008:administration & dosage / Q000627:therapeutic use*; D003876:Dermatitis, Atopic / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000188:drug therapy / Q000276:immunology*; D004285:Dogs; D004311:Double-Blind Method; D007073:Immunoglobulin E / Q000097:blood*; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000627:therapeutic use; D007428:Intradermal Tests / Q000662:veterinary
chemical_list: D000485:Allergens; D007166:Immunosuppressive Agents; D007073:Immunoglobulin E; D016572:Cyclosporine