[Complete genome sequencing of enterovirus 71 (EV71) HENAN08 strain isolated in Henan province].
OBJECTIVE
To sequence the whole-genome of enterovirus 71 (EV71) srtain isolated from patient with hand, foot and mouth in Henan province in 2008.


METHODS
Eight overlapping clones covering the whole viral genome were obtained by RT-PCR and the sequences were determined by Sanger dideoxg-mediated chain termination method.


RESULTS
Data it showed that the full length of enterovirus 71 (EV71) HENAN08 genome (not including Poly A tail) is 7405 bp. No deletion or insertion was detected in the coding region. There were several deletions and insertions in 5'UTR and 3'UTR regions. In P1 region, HENAN08 strain shared high homology with AnhuiFY08 strain, Zhejiang08 strain and SHZH strains (SHZH98, SHZH03) but low homology with Cox. A16. In P2 and P3 regions, HENAN08 strain shared higher nucleotide homology with Cox. A16 (81.7% and 83.7%) than that with BrCr and TW2086 strains. The phylogenetic analysis based on P1 region demonstrates that HENAN08 strain had the nearest genetic relationship with AnhuiFY and Zhejiang strains (isolated in 2008).


CONCLUSIONS
The HENAN08 strain might belong to the same genogroup with AnhuiFY08 and Zhejiang08 strains as C4 gene subtypes.
Zhang
Shao-li
SL
College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Xu
Bian-li
BL
Guo
Wan-shen
WS
Chen
Li
L
Wei
Hai-yan
HY
DU
Yan-hua
YH
Li
Xing-le
XL
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D002681:China; D004770:Enterovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D005838:Genotype; D006232:Hand, Foot and Mouth Disease / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions