title: [Study on the molecular characteristics of multidrug-resistant Acinetobacter baumannii].
abstract: OBJECTIVE
  To investigate antibiotic resistance, carbapenemase genotype and the molecular epidemiology of multidrug-resistant Acinetobacter baumannii (Aba) collected from 3 military hospitals in China.
  
  
  METHODS
  The minimum inhibitory concentrations (MIC) were examined by ager dilution method. Genotypes of carbapenemases were amplified by multiplex PCR and its products were sequenced. PCR was used to detect per gene. Homology of the resistant isolates was analyzed by pulse-field gel electrophoresis (PFGE).
  
  
  RESULTS
  Among the 64 MDRA strains, 78.1% (50) strains possessed bla(OXA-23) gene, 89.1% (57) carried Class 1 integrase gene, 39.1% (25) with bla(PER-1) gene, and 1 strain with bla(OXA-58-like) gene. PFGE showed that 13 (A, B, C, D, E genotype) different clones were identified in these strains. A, B, and U clones were the predominant clones in three hospitals, respectitively.
  
  
  CONCLUSIONS
  Outbreaks of multidrug-resistant Aba occurred at 3 military hospitals with the most prevalent carbapenemase as OXA-23 enzyme. OXA-58 type of carbapenemase and per-1 in Aba were also isolated.
authors.affiliation: Department of Microbiology, PLA General Hospital, Beijing 100853, China.
authors.forename: Jing-Rong
authors.initials: JR
authors.lastname: Cao
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.forename: Zhong-Qiang
authors.initials: ZQ
authors.lastname: Yan
authors.forename: Ding-Xia
authors.initials: DX
authors.lastname: Shen
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Luo
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006772:Hospitals, Military; D006801:Humans; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase