title: Array comparative genomic hybridization analysis of adult acute leukemia patients.
abstract: We have performed a retrospective array-based comparative hybridization (array-CGH) study on 41 acute leukemia samples [n=17 acute lymphoblastic leukemia (ALL) patients only at diagnosis, n=3 ALL patients both at diagnosis and relapse; n=20 acute myeloid leukemia (AML) patients only at diagnosis and n=1 AML patient both at diagnosis and relapse] using an Agilent 44K array. In addition to previously detected cytogenetic aberrations, we observed cryptic aberrations in 95% of ALL and 90.5% of AML cases. ALL-specific recurrent abnormalities were RB1 (n=3), PAX5 (n=4), and CDKN2B (n=3) deletions; AML-specific recurrent abnormalities were HOXA9 and HOXA10 (n=2) deletions and NOTCH1 duplication (n=2). Recurrent duplication of the ELK1 oncogene was observed in both ALL (n=2) and AML (n=3) cases. Our results demonstrate that oligo-array CGH (oaCGH) is an effective method for defining copy number alterations and identification of novel recurring unbalanced abnormalities. At least for now, however, the use of oaCGH for routine diagnosis still has some restrictions.
authors.affiliation: Department of Medical Biology and Genetics, Akdeniz University, Antalya 07508, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Yasar
authors.forename: Ihsan
authors.initials: I
authors.lastname: Karadogan
authors.forename: Guchan
authors.initials: G
authors.lastname: Alanoglu
authors.forename: Bahar
authors.initials: B
authors.lastname: Akkaya
authors.forename: Guven
authors.initials: G
authors.lastname: Luleci
authors.forename: Ozan
authors.initials: O
authors.lastname: Salim
authors.forename: Aysen
authors.initials: A
authors.lastname: Timuragaoglu
authors.forename: Gokce A
authors.initials: GA
authors.lastname: Toruner
authors.forename: Sibel
authors.initials: S
authors.lastname: Berker-Karauzum
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015470:Leukemia, Myeloid, Acute / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction