Staphylococcus aureus extracellular adherence protein contributes to biofilm formation in the presence of serum.
Staphylococcus aureus extracellular adherence protein (EAP) is secreted, but it can redock on the bacterial cell surface via neutral phosphatase (Nptase). EAP binds to certain blood proteins and to itself, and through these affinities, it contributes to adherence and aggregation. It has been demonstrated previously that EAP expression is iron regulated and it contributes to biofilm formation under iron-deplete conditions. In this study, we found that EAP and Nptase also play a role in biofilm formation under iron-replete conditions in the presence of human serum.
Thompson
Karl M
KM
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23928, USA.
Abraham
Nabil
N
Jefferson
Kimberly K
KK
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D006801:Humans; D007501:Iron / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000378:metabolism*; D044967:Serum / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000502:physiology*
D001426:Bacterial Proteins; C444739:Eap-N protein, Staphylococcus aureus; D016601:RNA-Binding Proteins; D007501:Iron; C092526:Staphylococcal neutral phosphatase; D010744:Phosphoric Monoester Hydrolases