title: Laudanosine has no effects on respiratory activity but induces non-respiratory excitement activity in isolated brainstem-spinal cord preparation of neonatal rats.
abstract: Laudanosine, a degradation of neuomuscular blocking agent atracurium, crosses the blood-brain barrier and is indicted to trigger seizures at high concentration. In Xenopus Oocytes expressing nicotinic acetylcholine receptors (nAChRs), laudanosine has activating and inhibiting effects on nAChRs depending on its concentration. nAChRs is related to respiratory activities and thus, in the present study, we analyzed effects of laudanosine on central respiratory activities using isolated brainstem-spinal cord preparation of neonatal rats. The rhythmic inspiratory burst activity of the C4 spinal ventral root was recorded using a glass suction electrode as an index of respiratory rate. After superfusion with mock cerebrospinal fluid (CSF), the preparation was superfused with mock CSF containing laudanosine 1, 10 or 100 microM for 60 minutes. Laudanosine 1, 10 and 100 microM (n = 10 in each) did not induce any effects on C4 respiratory rate. In all 10 preparations, laudnosine 100 microM induced non-respiratory excitement activities that are possibly same as seizure observed in vivo study.
authors.affiliation: Department of Anesthesiology, Keio University, Tokyo, Japan. shigekisakuraba@gmail.com
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sakuraba
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kaku
authors.forename: Junzo
authors.initials: J
authors.lastname: Takeda
authors.forename: Shun-ichi
authors.initials: S
authors.lastname: Kuwana
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001933:Brain Stem / Q000187:drug effects* / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015656:Respiratory Mechanics / Q000187:drug effects* / Q000502:physiology*; D013116:Spinal Cord / Q000187:drug effects* / Q000502:physiology*
chemical_list: D007546:Isoquinolines; C001522:laudanosine