title: Exogenous platelet-activating factor improves the motility of human spermatozoa evaluated with C.A.S.A.: optimal concentration and incubation time.
abstract: The objective of this study is to determine the optimal conditions for human semen incubation treated with exogenous platelet activating factor (ePAF) for intra-uterine insemination (IUI). This prospective study was carried out on 32 infertile men and each semen sample was processed with the ISolate Sperm Separation Medium, washed with sperm washing medium (SWM) and resuspended either in SWM alone (control samples), or with ePAF 0.1, 0.5, and 1.0 μM. Each concentration was subsequently incubated and evaluated at 5, 15, 30, and 60 min. The motility parameters were evaluated by the computer-aided sperm analysis (C.A.S.A.) system. Curvilinear velocity, straight line velocity, average path velocity, rapid and progressive motility significantly increased compared to control samples at an ePAF concentration of 0.1 μM (with at least 15 min of incubation). The best results were obtained with ePAF concentrations of: 0.1 μM (60 min of incubation) and 0.5 μM (30-60 min of incubation). In conclusion, results are enhanced when ePAF is added to standard semen preparation for IUI. An ePAF concentration of 0.1 μM, with an incubation time of 15 min, can be used for semen samples with normal motility. Whilst, for semen samples with poor motility, the ePAF concentration is best increased to 0.5 μM and/or the incubation time prolonged to 60 min.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Turin, Via Ventimiglia 3, 10126, Turin, Italy. giuseppinagrassi@alice.it
authors.forename: G
authors.initials: G
authors.lastname: Grassi
authors.forename: N
authors.initials: N
authors.lastname: Cappello
authors.forename: M F
authors.initials: MF
authors.lastname: Gheorghe
authors.forename: L
authors.initials: L
authors.lastname: Salton
authors.forename: C
authors.initials: C
authors.lastname: Di Bisceglie
authors.forename: C
authors.initials: C
authors.lastname: Manieri
authors.forename: C
authors.initials: C
authors.lastname: Benedetto
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D007315:Insemination, Artificial; D008297:Male; D008875:Middle Aged; D009994:Osmolar Concentration; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011247:Pregnancy; D018873:Pregnancy Rate; D055101:Semen Analysis / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors; D055815:Young Adult
chemical_list: D010972:Platelet Activating Factor