[Generation of sugar beet transgenic plants expressing bar gene].
The parameters of transformation using Agrobacterium tumefaciens EHA 105 for 5 domestic sorts and lines of sugar beet (Beta vulgaris L. var. saccharifera (Alef) Krass) were optimized. The system of transgenic tissue selection based on resistance to phosphinothricin, allowing to avoid the appearing of chimeric shoots among initial transformants was developed. The transgenic plants of sugar beet sorts Ramonskaya single seed 47, L'govskaya single seed 52 and RMS 73, and LBO 17 and LBO 19 lines expressing the gene of phosphinothricin acetyl transferase bar have been obtained. The resistance of these sorts and lines to the effect of phosphinothricin in vitro has been shown.
Mishutkina
Ia V
IaV
Kamionskaia
A M
AM
Skriabin
K G
KG
D000123:Acetyltransferases / Q000235:genetics*; D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000613:Aminobutyrates / Q000494:pharmacology; D027461:Beta vulgaris / Q000187:drug effects / Q000235:genetics*; D053209:Herbicide Resistance / Q000235:genetics*; D006540:Herbicides / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics*; D014170:Transformation, Genetic
D000613:Aminobutyrates; D006540:Herbicides; C003121:phosphinothricin; D000123:Acetyltransferases; C056243:phosphinothricin N-acetyltransferase