[DNA-EGS1386 in cells induced RNase P inhibits the expression of human cytomegalovirus UL49 gene].
External Guide Sequences (EGSs) represents a novel nucleic acid based gene interference approach to modulate gene expression. They are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA. DNA-based EGS1386 with a size of 12 nt was chemically synthesized to target the mRNA coding for the UL49 gene of human cytomegalovirus (HCMV). The DNA-based EGS1386 molecule efficiently directed human RNase P to cleave the target mRNA sequence in vitro. A reduction of more than 50% in the levels of UL49 expression was observed in human cells treated with the DNA-based EGS1386 targeted UL49 assayed by fluorescent quantization PCR and Western blotting. This results showed that the DNA-EGS1386 can effectively guide the RNase P cut the target mRNA. Therefore, DNA-EGS can develop into a new gene silencing technology and potential of the anti-viral reagents.
Cui
Yanwei
Y
National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Zeng
Zhifeng
Z
Li
Hongjian
H
Li
Yueqin
Y
Zhou
Qi
Q
Yang
Dan
D
Zou
Yi
Y
Yang
Guang
G
Zhou
Tianhong
T
D001483:Base Sequence; D003587:Cytomegalovirus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D003586:Cytomegalovirus Infections / Q000201:enzymology / Q000821:virology; D004279:DNA, Viral / Q000235:genetics*; D019020:Directed Molecular Evolution / Q000379:methods; D015967:Gene Expression Regulation, Viral; D006801:Humans; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043262:Ribonuclease P / Q000235:genetics / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism; D058727:RNA, Small Untranslated
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D015678:Viral Structural Proteins; D043262:Ribonuclease P; D058727:RNA, Small Untranslated