[Establishment of the feeding methodology of Aedes aegypti (Diptera-Culicidae) in Swiss mice and evaluation of the toxicity and residual effect of essential oil from Tagetes minuta L (Asteraceae), in populations of Aedes aegypti].
The objectives here were to develop a procedure for feeding females of Aedes aegypti that does not cause stress in Swiss mice and to evaluate the toxicity and residual effect of essential oil from Tagetes minuta L. (Asteraceae) in Aedes aegypti populations. Two mice were anesthetized: one was used to observe the duration of sedation and the other was placed in a cage to feed the female mosquitoes. Essential oil was diluted in acetone and used in bioassays to assess the lethal concentrations in larvae from the Cities of Bauru (SP) and São José do Rio Preto (SP) that were sensitive and resistant to temephos, respectively. The data obtained were compared with the American Rockefeller strain. The procedure with mice was approved. There was no difference between the populations regarding susceptibility to Tagetes minuta, and the assays showed LC50 of 0.24, 0.25 and 0.21 ml/l and LC99.9 of 0.35, 0.39 and 0.42 ml/l, for Rockefeller, Bauru and São José do Rio Preto, respectively. The solution did not show any residual effect.
Lima
Waldemir Pereira
WP
Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP.
Chiaravalloti Neto
Francisco
F
Macoris
Maria de Lourdes da Graça
Mde L
Zuccari
Débora Aparecida Pires de Campos
DA
Dibo
Margareth Regina
MR
D000330:Aedes; D000818:Animals; D005247:Feeding Behavior / Q000502:physiology; D005260:Female; D007814:Larva; D051379:Mice; D009822:Oils, Volatile; D010573:Pesticide Residues; D010936:Plant Extracts; D010938:Plant Oils; D031686:Tagetes / Q000737:chemistry*
D009822:Oils, Volatile; D010573:Pesticide Residues; D010936:Plant Extracts; D010938:Plant Oils