title: Magnetomotive laser speckle imaging.
abstract: Laser speckle imaging (LSI) involves analysis of reflectance images collected during coherent optical excitation of an object to compute wide-field maps of tissue blood flow. An intrinsic limitation of LSI for resolving microvascular architecture is that its signal depends on relative motion of interrogated red blood cells. Hence, with LSI, small-diameter arterioles, venules, and capillaries are difficult to resolve due to the slow flow speeds associated with such vasculature. Furthermore, LSI characterization of subsurface blood flow is subject to blurring due to scattering, further limiting the ability of LSI to resolve or quantify blood flow in small vessels. Here, we show that magnetic activation of superparamagnetic iron oxide (SPIO) nanoparticles modulate the speckle flow index (SFI) values estimated from speckle contrast analysis of collected images. With application of an ac magnetic field to a solution of stagnant SPIO particles, an apparent increase in SFI is induced. Furthermore, with application of a focused dc magnetic field, a focal decrease in SFI values is induced. Magnetomotive LSI may enable wide-field mapping of suspicious tissue regions, enabling subsequent high-resolution optical interrogation of these regions. Similarly, subsequent photoactivation of intravascular SPIO nanoparticles could then be performed to induce selective photothermal destruction of unwanted vasculature.
authors.affiliation: Kyungpook National University, Electrical and Computer Engineering, Daegu, South Korea.
authors.forename: Jeehyun
authors.initials: J
authors.lastname: Kim
authors.forename: Junghwan
authors.initials: J
authors.lastname: Oh
authors.forename: Bernard
authors.initials: B
authors.lastname: Choi
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000737:chemistry*; D004574:Electromagnetic Fields; D005290:Ferric Compounds; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D053685:Laser Therapy; D017078:Laser-Doppler Flowmetry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008833:Microcirculation; D008955:Models, Cardiovascular; D019047:Phantoms, Imaging; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide