title: Genotypic linkages of VP4, VP6, VP7, NSP4, NSP5 genes of rotaviruses circulating among children with acute gastroenteritis in Thailand.
abstract: Rotavirus is the main cause of acute viral gastroenteritis in infants and young children worldwide. Surveillance of group A rotavirus has been conducted in Chiang Mai, Thailand since 1987 up to 2004 and those studies revealed that group A rotavirus was responsible for about 20-61% of diarrheal diseases in hospitalized cases. In this study, we reported the continuing surveillance of group A rotavirus in 2005 and found that group A rotavirus was detected in 43 out of 147 (29.3%) stool samples. Five different G and P genotype combinations were detected, G1P[8] (27 strains), G2P[4] (12 strains), G9P[8] (2 strains), G3P[8] (1 strain), and G3P[10] (1 strain). In addition, analysis of their genotypic linkages of G (VP7), P (VP4), I (VP6), E (NSP4), and H (NSP5) genotypes demonstrated that the rotaviruses circulating in Chiang Mai, Thailand carried 3 unique linkage patterns. The G1P[8], G3P[8], and G9P[8] strains carried their VP6, NSP4, NSP5 genotypes of I1, E1, H1, respectively. The G2P[4] strains were linked with I2, E2, H2 genotypes, while an uncommon G3P[10] genotype carried unique genotypes of I8, E3 and H6. These findings provide the overall picture of genotypic linkage data of rotavirus strains circulating in Chiang Mai, Thailand.
authors.affiliation: Aino Health Science Center, Aino University, Tokyo, Japan.
authors.forename: Pattara
authors.initials: P
authors.lastname: Khamrin
authors.forename: Niwat
authors.initials: N
authors.lastname: Maneekarn
authors.forename: Rungnapa
authors.initials: R
authors.lastname: Malasao
authors.forename: Tuan Anh
authors.initials: TA
authors.lastname: Nguyen
authors.forename: Shinichi
authors.initials: S
authors.lastname: Ishida
authors.forename: Shoko
authors.initials: S
authors.lastname: Okitsu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D000208:Acute Disease; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics*; D002675:Child, Preschool; D005759:Gastroenteritis / Q000821:virology*; D008040:Genetic Linkage / Q000235:genetics*; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D007223:Infant; D010802:Phylogeny; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000821:virology*; D013785:Thailand; D014118:Toxins, Biological / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; C060339:VP4 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins