Comparative proteomic analysis of whole saliva from chronic periodontitis patients.
Chronic periodontal disease is a chronic inflammatory process affecting tooth supporting tissues in the presence of pathogenic bacterial biofilm. There is some evidence for changes in the protein composition of whole saliva from chronic periodontitis patients, but there have been no studies using a proteomic approach. Hence, the aim of this study was to compare the protein profiles of unstimulated whole saliva from patients with periodontitis and healthy subjects by two complementary approaches (2D-gel electrophoresis and liquid chromatography). Protein spots of interest were analyzed by MALDI-TOF-TOF, and the data was complemented by an ESI-Q-TOF experiment. The analyses revealed that subjects with periodontal disease have increased amounts of blood proteins (serum albumin and hemoglobin) and immunoglobulin, and they have a lower abundance of cystatin compared to the control group. A higher number of protein spots were observed in the periodontitis group, of which most were identified as alpha-amylase. This higher number of alpha-amylase variants seems to be caused by hydrolysis by cysteine proteases under such inflammatory conditions. This approach gives novel insights into alterations of salivary protein in presence of periodontal inflammation and may contribute to the improvement of periodontal diagnosis.
Gonçalves
Lorena Da Rós
Lda R
Laboratório de Genética Aplicada, Instituto Nacional de Câncer, INCA, Rio de Janeiro, Brazil.
Soares
Márcia Regina
MR
Nogueira
Fábio C S
FC
Garcia
Carlos
C
Camisasca
Danielle Resende
DR
Domont
Gilberto
G
Feitosa
Alfredo C R
AC
Pereira
Denise de Abreu
Dde A
Zingali
Russolina B
RB
Alves
Gilda
G
D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000175:diagnosis* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D012463:Saliva / Q000737:chemistry*
D015415:Biomarkers; D020543:Proteome