Electrochemical DNA sandwich assay with a lipase label for attomole detection of DNA.
A fast and sensitive electrochemical lipase-based sandwich hybridization assay for detection of attomole levels of DNA has been developed. A combination of magnetic beads, used for pre-concentration and bioseparation of the analyte with a lipase catalyst label allowed detection of DNA with a limit of 20 amol.
Ferapontova
Elena E
EE
Centre for DNA Nanotechnology, Department of Chemistry and iNANO, Aarhus University, Langelandsgade 140, DK-8000 Aarhus C, Denmark. e_ferapontova@chem.au.dk
Hansen
Majken N
MN
Saunders
Aaron M
AM
Shipovskov
Stepan
S
Sutherland
Duncan S
DS
Gothelf
Kurt V
KV
D004247:DNA / Q000032:analysis* / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D008280:Magnetics; D020411:Oligonucleotide Array Sequence Analysis
D006046:Gold; D004247:DNA; D008049:Lipase