A critical role for the g protein-coupled receptor mFPR2 in airway inflammation and immune responses.
The formylpeptide receptor-like 1, now officially termed FPR2, in human and its mouse homolog mFPR2 mediate leukocyte migration in response to agonists associated with inflammation and immune responses. To clarify the in vivo role of the receptor, we generated mice deficient in mFPR2. mFPR2(-/-) mice showed markedly reduced severity in OVA/alum-induced allergic airway inflammation. This was associated with diminished recruitment of CD11c(+) dendritic cells into the airway mucosa and secondary lymphoid organs, as well as reduced production of Type 2 cytokines and Igs. We also found that the bronchoalveolar lavage fluid from wild type mice with airway inflammation contained mFPR2 agonist activity. This study reveals a critical role for mFPR2 in the progression of allergic airway inflammation and immune responses.
Chen
Keqiang
K
Laboratory of Molecular Immunoregulation, Center for Cancer Research, National Cancer Institute at Frederick, Maryland, USA.
Le
Yingying
Y
Liu
Ying
Y
Gong
Wanghua
W
Ying
Guoguang
G
Huang
Jian
J
Yoshimura
Teizo
T
Tessarollo
Lino
L
Wang
Ji Ming
JM
D000485:Allergens / Q000276:immunology / Q000494:pharmacology; D000534:Alum Compounds / Q000494:pharmacology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000276:immunology; D002469:Cell Separation; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006967:Hypersensitivity / Q000276:immunology*; D007150:Immunohistochemistry; D007249:Inflammation / Q000276:immunology* / Q000473:pathology; D008168:Lung / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D044042:Receptors, Formyl Peptide / Q000276:immunology*; D012130:Respiratory Hypersensitivity / Q000276:immunology* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000485:Allergens; D000534:Alum Compounds; D016207:Cytokines; D044042:Receptors, Formyl Peptide; C481578:formyl peptide receptor 2, mouse; C041524:aluminum sulfate; D010047:Ovalbumin