title: [Involvement of endoplasmic reticulum stress in development of fatty liver fibrosis induced by methionine-choline-deficient diet in rats].
abstract: OBJECTIVE
  To study role of endoplasmic reticulum stress in the development of fatty liver fibrosis induced by methionine-choline-deficient diet in rats.
  
  
  METHODS
  Non-alcoholic steatohepatitis was induced by 10 weeks- methionine-choline-deficient diet (MCDD), Markers of endoplasmic reticulum stress were determined by immunoblotting and real-time PCR.
  
  
  RESULTS
  The number of apoptotic hepatocytes, The expression levels of endoplasmic reticulum stress markers were increased significantly in MCDD group compared to control group (probability value less than 0.05 or probability value less than 0.01), while ratio of hepatocyte proliferation/apoptosis was decreased in MCDD group (probability value less than 0.01). The number of hepatocytes apoptosis, and the expression levels of endoplasmic reticulum stress markers were decreased significantly 2 weeks after the feeding with normal diet in MCDD group (probability value less than 0.05 or probability value less than 0.01).
  
  
  CONCLUSIONS
  MCDD induces endoplasmic reticulum stress and fibrosis in rats.
authors.affiliation: Shanghai Public Health Clinical Centre, Shanghai 201508, China. ypmu8888@126.com
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: Mu
authors.forename: Norifumi
authors.initials: N
authors.lastname: Kwada
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ogawa
authors.forename: Xiu-hong
authors.initials: XH
authors.lastname: Xi
authors.forename: Xiao-rong
authors.initials: XR
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D002794:Choline / Q000008:administration & dosage / Q000378:metabolism; D002796:Choline Deficiency; D004032:Diet; D004195:Disease Models, Animal; D004721:Endoplasmic Reticulum / Q000502:physiology*; D005234:Fatty Liver / Q000150:complications*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000178:diet therapy* / Q000209:etiology / Q000503:physiopathology*; D008297:Male; D008715:Methionine / Q000172:deficiency; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D012333:RNA, Messenger; D008715:Methionine; D020169:Caspases; D002794:Choline