title: Interaction between serotonin 5-HT2A receptor gene and dopamine transporter (DAT1) gene polymorphisms influences personality trait of persistence in Austrian Caucasians.
abstract: We examined 89 normal volunteers using Cloninger's Temperament and Character Inventory (TCI). Genotyping the 102T/C polymorphism of the serotonin 5HT2A receptor gene and the ser9gly polymorphism in exon 1 of the dopamine D3 receptor (DRD3) gene was performed using PCR-RFLP, whereas the dopamine transporter (DAT1) gene variable number of tandem repeats (VNTR) polymorphism was investigated using PCR amplification followed by electrophoresis in an 8% acrylamide gel with a set of size markers. We found a nominally significant association between gender and harm avoidance (P=0.017; women showing higher scores). There was no association of either DAT1, DRD3 or 5HT2A alleles or genotypes with any dimension of the TCI applying Kruskal-Wallis rank-sum tests. Comparing homozygote and heterozygote DAT1 genotypes, we found higher novelty seeking scores in homozygotes (P=0.054). We further found a nominally significant interaction between DAT1 and 5HT2A homo-/heterozygous gene variants (P=0.0071; DAT1 and 5HT2A genotypes P value of 0.05), performing multivariate analysis of variance (MANOVA). Examining the temperamental TCI subscales, this interaction was associated with persistence (genotypes: P=0.004; homo-/heterozygous gene variants: P=0.0004). We conclude that an interaction between DAT1 and 5HT2A genes might influence the temperamental personality trait persistence.
authors.affiliation: Division of Biological Psychiatry, Department of Psychiatry & Psychotherapy, Medical University Vienna, Vienna, Austria. alexandra.schosser@meduniwien.ac.at
authors.forename: Alexandra
authors.initials: A
authors.lastname: Schosser
authors.forename: Karoline
authors.initials: K
authors.lastname: Fuchs
authors.forename: Theresa
authors.initials: T
authors.lastname: Scharl
authors.forename: Monika
authors.initials: M
authors.lastname: Schloegelhofer
authors.forename: Jochen
authors.initials: J
authors.lastname: Kindler
authors.forename: Nilufar
authors.initials: N
authors.lastname: Mossaheb
authors.forename: Rainer M
authors.initials: RM
authors.lastname: Kaufmann
authors.forename: Friedrich
authors.initials: F
authors.lastname: Leisch
authors.forename: Siegfried
authors.initials: S
authors.lastname: Kasper
authors.forename: Werner
authors.initials: W
authors.lastname: Sieghart
authors.forename: Harald N
authors.initials: HN
authors.lastname: Aschauer
mesh_terms: D000328:Adult; D000483:Alleles; D001317:Austria; D050483:Dopamine Plasma Membrane Transport Proteins / Q000235:genetics*; D005106:Exploratory Behavior; D005260:Female; D005838:Genotype; D040261:Harm Reduction; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics; D010551:Personality / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D044402:Receptor, Serotonin, 5-HT2A / Q000235:genetics*; D050637:Receptors, Dopamine D3 / Q000235:genetics; D012737:Sex Factors; D018709:Statistics, Nonparametric; D044465:White People / Q000235:genetics* / Q000523:psychology
chemical_list: C493593:DRD3 protein, human; D050483:Dopamine Plasma Membrane Transport Proteins; D044402:Receptor, Serotonin, 5-HT2A; D050637:Receptors, Dopamine D3; C493214:SLC6A3 protein, human