[Phylogenetic analysis of symbiotic archaea in the gut of Reticulitermes chinensis Snyder].
OBJECTIVE
To analyze the phylotypes of symbiotic archaea in the gut of Reticulitermes chinensis Snyder by using non-cultivating method.


METHODS
We amplified 16S rRNA genes of the symbiotic archaea from the DNA extract of the whole gut with the universal archaeal 16S rDNA primer. A clone library of archaeal 16S rDNA was established and selected clones were sequenced and phylogeneticly analyzed.


RESULTS
Five 16S rDNA sequences with the similarity between 93.2% and 99.2% to each other were obtained from Reticulitermes chinensis Snyder. Phylogenetic analysis showed that the five clones were clustered with Methanobrevibacter clones or isolates obtained from Reticulitermes speratus and Reticulitermes flavipes.


CONCLUSIONS
The results showed that symbiotic archaea in the gut of Reticulitermes chinensis were affiliated with Methanobrevibacter.
Shi
Yu
Y
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China. shiy2005@126.com
Zhang
Yanhong
Y
Yang
Hong
H
D000818:Animals; D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007422:Intestines / Q000382:microbiology; D020049:Isoptera / Q000382:microbiology* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013559:Symbiosis
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S