title: Highly biocompatible and water-dispersible, amine functionalized magnetite nanoparticles, prepared by a low temperature, air-assisted polyol process: a new platform for bio-separation and diagnostics.
abstract: A low temperature polyol process, based on glycolaldehyde mediated partial reduction of FeCl(3).6H(2)O at 120 degrees C in the presence of sodium acetate as an alkali source and 2, 2(')-(ethylenedioxy)-bis-(ethylamine) as an electrostatic stabilizer has been used for the gram-scale preparation of biocompatible, water-dispersible, amine functionalized magnetite nanoparticles (MNPs) with an average diameter of 6 +/- 0.75 nm. With a reasonably high magnetization (37.8 e.m.u.) and amine groups on the outer surface of the nanoparticles, we demonstrated the magnetic separation and concentration implications of these ultrasmall particles in immunoassay. MRI studies indicated that these nanoparticles had the desired relaxivity for T(2) contrast enhancement in vivo. In vitro biocompatibility, cell uptake and MR imaging studies established that these nanoparticles were safe in clinical dosages and by virtue of their ultrasmall sizes and positively charged surfaces could be easily internalized by cancer cells. All these positive attributes make these functional nanoparticles a promising platform for further in vitro and in vivo evaluations.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, India. md_manasmita@yahoo.com
authors.forename: Manasmita
authors.initials: M
authors.lastname: Das
authors.forename: Prasanta
authors.initials: P
authors.lastname: Dhak
authors.forename: Satyajit
authors.initials: S
authors.lastname: Gupta
authors.forename: Debasish
authors.initials: D
authors.lastname: Mishra
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Amit
authors.initials: A
authors.lastname: Basak
authors.forename: Panchanan
authors.initials: P
authors.lastname: Pramanik
mesh_terms: D000588:Amines / Q000737:chemistry* / Q000493:pharmacokinetics; D001672:Biocompatible Materials / Q000737:chemistry* / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D055598:Chemical Phenomena; D003080:Cold Temperature; D052203:Ferrosoferric Oxide / Q000737:chemistry* / Q000493:pharmacokinetics; D006367:HeLa Cells; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism; D058085:Iron Compounds / Q000737:chemistry; D008279:Magnetic Resonance Imaging; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D019047:Phantoms, Imaging; D011108:Polymers / Q000737:chemistry*; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D000588:Amines; D001672:Biocompatible Materials; D007074:Immunoglobulin G; D058085:Iron Compounds; D011108:Polymers; C024617:polyol; D014867:Water; D052203:Ferrosoferric Oxide