The first isoform-selective protein biosensor: a metallothionein potentiometric electrode.
The construction of a biosensor membrane by embedding mammalian Zn(7)-MT1 complexes as ionophores in a polysulfone matrix resulted in precise, accurate and, significantly, selective electrodes for MT1 quantification, so that the presence of other mammalian MT isoforms did not interfere in MT1 measurement.
Capdevila
Mercè
M
Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Barcelona, Spain. merce.capdevila@uab.cat
González-Bellavista
Anna
A
Muñoz
Maria
M
Atrian
Sílvia
S
Fàbregas
Esteve
E
D000595:Amino Acid Sequence; D000818:Animals; D015374:Biosensing Techniques; D004566:Electrodes; D008668:Metallothionein / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D011199:Potentiometry; D020033:Protein Isoforms / Q000032:analysis* / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
D020033:Protein Isoforms; D008668:Metallothionein