Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions.
We describe a Toxoplasma gondii strain that will permit the use of site-specific recombination to study the host-parasite interactions of this organism. This Toxoplasma strain efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in 95% of infected host cells. By infecting Cre-reporter mice with these parasites, we also monitored host-cell infection in vivo.
Koshy
Anita A
AA
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.
Fouts
Ashley E
AE
Lodoen
Melissa B
MB
Alkan
Ozan
O
Blau
Helen M
HM
Boothroyd
John C
JC
D000818:Animals; D006790:Host-Parasite Interactions; D019426:Integrases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D014122:Toxoplasma / Q000201:enzymology* / Q000235:genetics; D014123:Toxoplasmosis / Q000469:parasitology*; D014161:Transduction, Genetic
C045073:Cre recombinase; D019426:Integrases