title: Evaluation of formalin inactivated V3526 virus with adjuvant as a next generation vaccine candidate for Venezuelan equine encephalitis virus.
abstract: V3526, a genetically modified strain of Venezuelan equine encephalitis virus (VEEV), was formalin inactivated for evaluation as a next generation vaccine candidate for VEEV. In this study, we tested formalin-inactivated V3526 (fV3526) with and without adjuvant for immunogenicity and efficacy in BALB/c mice and results were compared to the existing inactivated VEEV vaccine, C84. Mice were vaccinated intramuscularly (IM) or subcutaneously (SC) with fV3526 formulations and challenged with VEEV IAB Trinidad donkey (VEEV TrD) strain by SC or aerosol exposure. Efficacy following SC or aerosol challenge was not significantly different between the fV3526 formulations or compared to C84 despite C84 being administered in more doses and higher concentration of viral protein per dose. These data support further evaluation of fV3526 formulations as a next generation VEEV vaccine.
authors.affiliation: DynPort Vaccine Company LLC, A CSC Company, 64 Thomas Johnson Drive, Frederick, MD 21702, USA. smartin40@csc.com
authors.forename: Shannon S
authors.initials: SS
authors.lastname: Martin
authors.forename: Russell R
authors.initials: RR
authors.lastname: Bakken
authors.forename: Cathleen M
authors.initials: CM
authors.lastname: Lind
authors.forename: Patricia
authors.initials: P
authors.lastname: Garcia
authors.forename: Erin
authors.initials: E
authors.lastname: Jenkins
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Glass
authors.forename: Michael D
authors.initials: MD
authors.lastname: Parker
authors.forename: Mary Kate
authors.initials: MK
authors.lastname: Hart
authors.forename: Donald L
authors.initials: DL
authors.lastname: Fine
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D004202:Disinfectants / Q000494:pharmacology; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics / Q000276:immunology*; D004685:Encephalomyelitis, Venezuelan Equine / Q000517:prevention & control; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology; D007273:Injections, Intramuscular; D007279:Injections, Subcutaneous; D051379:Mice; D008807:Mice, Inbred BALB C; D016019:Survival Analysis; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D004202:Disinfectants; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D005557:Formaldehyde