title: RsaOG, a new staphylococcal family of highly transcribed non-coding RNA.
abstract: The expression of trans-acting small RNAs in firmicutes has been poorly documented to date. This gap is being filled quickly in the genus Staphylococcus, which is both a model firmicute and an important human pathogen. Here we analyze RsaOG, a novel small RNA family specific to Staphylococcus and highly transcribed. This well conserved element, first discovered in a computational screen, was precisely mapped in the genome by RACE mapping and the identification of a putative transcriptional promoter. The proposed secondary structure presents two highly conserved unpaired sequences, part of which can form a pseudoknot. We suggest a possible involvement of the remaining conserved single stranded region in trans regulatory interactions.
authors.affiliation: Institut de Génétique et Microbiologie, CNRS/UMR 8621, Université Paris Sud, Orsay, France.
authors.forename: Antonin
authors.initials: A
authors.lastname: Marchais
authors.forename: Chantal
authors.initials: C
authors.lastname: Bohn
authors.forename: Philippe
authors.initials: P
authors.lastname: Bouloc
authors.forename: Daniel
authors.initials: D
authors.lastname: Gautheret
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic / Q000235:genetics; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D022661:RNA, Untranslated / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D013211:Staphylococcus aureus / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012329:RNA, Bacterial; D022661:RNA, Untranslated