title: High-resolution tomographic diffractive microscopy of biological samples.
abstract: The authors have developed a tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens, with high lateral resolution. The authors now study its use for biological applications, and imaged several preparations with fluorescence confocal microscopy and tomographic diffractive microscopy. The results highlight some interesting features of this instrument, which should attract the interest of biologists for this new technique.
authors.affiliation: Laboratoire MIPS-EA2332, Université de Haute-Alsace, IUT Mulhouse, Mulhouse, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Simon
authors.forename: Matthieu
authors.initials: M
authors.lastname: Debailleul
authors.forename: Anne
authors.initials: A
authors.lastname: Beghin
authors.forename: Yves
authors.initials: Y
authors.lastname: Tourneur
authors.forename: Olivier
authors.initials: O
authors.lastname: Haeberlé
mesh_terms: D000042:Absorption; D002138:Calibration; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000737:chemistry / Q000821:virology; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000166:cytology; D006696:Holography / Q000379:methods*; D006801:Humans; D021621:Imaging, Three-Dimensional; D053122:Influenza A Virus, H3N2 Subtype; D008853:Microscopy / Q000379:methods*; D009055:Mouth / Q000166:cytology; D014054:Tomography / Q000379:methods*