title: Concerto catalysis--harmonising [NiFe]hydrogenase and NiRu model catalysts.
abstract: This communication reports the successful merging of the chemical properties of a natural [NiFe]hydrogenase (Desulfovibrio vulgaris Miyazaki F) and our previously reported [NiRu] hydrogenase-mimic. The catalytic activity of both the natural enzyme and the mimic is almost identical, with the exception of working pH ranges, and this allows us to use them simultaneously in the same reaction flask. In such a manner, isotope exchange between D(2) and H(2)O could be conducted over an extended pH range (about 2-10) in one pot under mild conditions at ambient temperature and pressure.
authors.affiliation: Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Ichikawa
authors.forename: Kyoshiro
authors.initials: K
authors.lastname: Nonaka
authors.forename: Takahiro
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Bunsho
authors.initials: B
authors.lastname: Kure
authors.forename: Ki-Seok
authors.initials: KS
authors.lastname: Yoon
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Higuchi
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Yagi
authors.forename: Seiji
authors.initials: S
authors.lastname: Ogo
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D016969:Desulfovibrio vulgaris / Q000201:enzymology; D006859:Hydrogen / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006864:Hydrogenase / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D011487:Protein Conformation; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D012428:Ruthenium; D006859:Hydrogen; C043296:nickel-iron hydrogenase; D006864:Hydrogenase