Exploring different strategies to express Dengue virus envelope protein in a plant system.
Dengue virus envelope glycoprotein (E-protein) is the main protein associated with immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies.
Martínez
Carolina Andrea
CA
Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, CP 1113, Buenos Aires, Argentina.
Topal
Emel
E
Giulietti
Ana María
AM
Talou
Julián Rodríguez
JR
Mason
Hugh
H
D000914:Antibodies, Viral / Q000276:immunology; D019677:Artificial Gene Fusion; D001709:Biotechnology / Q000379:methods*; D015870:Gene Expression; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D017384:Sequence Deletion; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*; D019076:Transgenes; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; C051821:E-glycoprotein, Dengue virus type 2; D006512:Hepatitis B Core Antigens; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins