title: Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions.
abstract: We describe a Toxoplasma gondii strain that will permit the use of site-specific recombination to study the host-parasite interactions of this organism. This Toxoplasma strain efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in 95% of infected host cells. By infecting Cre-reporter mice with these parasites, we also monitored host-cell infection in vivo.
authors.affiliation: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Anita A
authors.initials: AA
authors.lastname: Koshy
authors.forename: Ashley E
authors.initials: AE
authors.lastname: Fouts
authors.forename: Melissa B
authors.initials: MB
authors.lastname: Lodoen
authors.forename: Ozan
authors.initials: O
authors.lastname: Alkan
authors.forename: Helen M
authors.initials: HM
authors.lastname: Blau
authors.forename: John C
authors.initials: JC
authors.lastname: Boothroyd
mesh_terms: D000818:Animals; D006790:Host-Parasite Interactions; D019426:Integrases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D014122:Toxoplasma / Q000201:enzymology* / Q000235:genetics; D014123:Toxoplasmosis / Q000469:parasitology*; D014161:Transduction, Genetic
chemical_list: C045073:Cre recombinase; D019426:Integrases