Cell culture and motility study on a polymer surface with a nanometer-scaled stripe structure.
We developed a large cell culture surface with a nanostripe structure by paving polydimethylsiloxane (PDMS) replicas of a glass mold. The stripe structure has a height of 180 nm and top width of 500 nm with 400-nm intervals between stripes. Human stomach cancer SH-10-TC cells cultured on the surface changed their morphology to elongated shapes parallel to the nanostripes. In addition, cell motility parallel to the stripes was greatly enhanced. These findings strongly suggest that the nanostripe structure affected the cell physiology.
Sakamoto
Yasuyuki
Y
Department of Applied Biological Chemistry, School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Sato
Kiichi
K
Abo
Mitsuru
M
Tsukahara
Takehiko
T
Kitamori
Takehiko
T
Abe
Keiko
K
Yoshimura
Etsuro
E
D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002465:Cell Movement; D004129:Dimethylpolysiloxanes / Q000138:chemical synthesis / Q000737:chemistry*; D005898:Glass; D006801:Humans; D049329:Nanostructures; D014407:Tumor Cells, Cultured
D004129:Dimethylpolysiloxanes