title: Biocompatibility and antibacterial effect of silver doped 3D-glass-ceramic scaffolds for bone grafting.
abstract: A 3D-glass-ceramic scaffold for bone tissue engineering with an interconnected macroporous network of pores was doped with silver ions in order to confer antibacterial properties. For this purpose, silver ions were selectively added to the scaffold surfaces through ion-exchange using an aqueous silver nitrate solution. The silver-doped scaffolds were characterized by means of leaching, in vitro antibacterial, and citotoxicity tests. In particular, the silver effect was examined through a broth dilution test in order to evaluate the proliferation of bacteria by counting the colonies forming units. Moreover, cytotoxicity tests were carried out to understand the effect of silver-containing scaffolds on cell adhesion, proliferation, and vitality. For all tests a comparison between silver-doped scaffold and silver-doped scaffold dry sterilized was performed.
authors.affiliation: Politecnico di Torino, Materials Science and Chemical Engineering Department, Corso Duca degli Abruzzi 24,10129 Turin, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Balagna
authors.forename: Chiara
authors.initials: C
authors.lastname: Vitale-Brovarone
authors.forename: Marta
authors.initials: M
authors.lastname: Miola
authors.forename: Enrica
authors.initials: E
authors.lastname: Verné
authors.forename: Rosa Angela
authors.initials: RA
authors.lastname: Canuto
authors.forename: Silvia
authors.initials: S
authors.lastname: Saracino
authors.forename: Giuliana
authors.initials: G
authors.lastname: Muzio
authors.forename: Giacomo
authors.initials: G
authors.lastname: Fucale
authors.forename: Giovanni
authors.initials: G
authors.lastname: Maina
mesh_terms: D000891:Anti-Infective Agents, Local / Q000032:analysis / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000494:pharmacology*; D016025:Bone Transplantation / Q000379:methods*; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D005898:Glass / Q000737:chemistry*; D007474:Ion Exchange; D008422:Materials Testing / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D012834:Silver / Q000494:pharmacology; D012835:Silver Nitrate / Q000032:analysis / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D000891:Anti-Infective Agents, Local; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D012834:Silver; D012835:Silver Nitrate