Simple detection of protein soft structure changes.
We present a rapid method for protein tertiary structure analysis which avoids the need for techniques such as circular dichroism and differential scanning calorimetry. Small changes to a protein's noncovalent "soft" structure are detected by exploiting differences in thermal stability and fluorescent reporter binding. It can detect subtle stability differences using micrograms of protein in 2 microL volumes within minutes.
Chalton
David A
DA
Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle-upon-Tyne, NE2 4HH, UK.
Lakey
Jeremy H
JH
D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism / Q000379:methods*; D003087:Colicins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature
D003087:Colicins; D005456:Fluorescent Dyes; D011506:Proteins