title: Assessing a shift of glucose biotransformation by LC-MS/MS-based metabolome analysis in carbon monoxide-exposed cells.
abstract: Carbon monoxide (CO) is the stress-inducible gas generated by heme oxygenase (HO). Although the HO/CO system appears to contribute to cell protection and tissue repair under stress conditions, its mode of actions remains largely unknown. We hypothesized that CO might alter the cellular energetic conditions and thereby modulate oxygen metabolism. To examine this hypothesis, we attempted to establish a method to follow the global flux of (13)C-glucose in the cells using metabolomic approaches with liquid chromatography-mass spectrometry (LC-MS/MS). The human monoblastic leukemia cell line U937 was exposed to the CO-releasing molecule (CORM). The CO exposure attenuated the conversion of the mass-labeled glucose to its downstream metabolites, while significantly stimulating its conversion to those for pentose phosphate pathway, suggesting roles of stress-inducible CO in a shift of glucose biotransformation.
authors.affiliation: Department of Biochemistry & Integrative Medical Biology, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: Naoharu
authors.initials: N
authors.lastname: Takano
authors.forename: Takehiro
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Adachi
authors.forename: Makoto
authors.initials: M
authors.lastname: Suematsu
mesh_terms: D001711:Biotransformation / Q000187:drug effects; D002247:Carbon Isotopes; D002248:Carbon Monoxide / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid / Q000379:methods*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome / Q000187:drug effects*
chemical_list: D002247:Carbon Isotopes; D002248:Carbon Monoxide; D005947:Glucose