[Experimental study on effects of Heparin II domain of fibronectin on cultured human trabecular meshwork cells].
OBJECTIVE
To determine the effects of Heparin II (Hep II) domain on cultured human trabecular meshwork (HTM) cells.


METHODS
HTM cells were cultured and treated with Hep II domain for 18 and 24 h. The morphological changes in HTM cells were assessed by light and electron microscopy. Changes in cell morphology and the organization of actin cytoskeleton, Vinculin, beta-Catenin were assessed by using immunofluorescence.


RESULTS
Treatment of Hep II domains resulted in morphological changes from 10 to 24 h. In light microscopy, cells rounded up, retracted and detached from each other. In high performance liquid chromatography, Hep II domains-treated cells showed that actin fibre bundles were highly concentrated at the periphery of the cells with few actin filaments left in this area; decreased vinculin staining was observed toward the cell periphery; decreased beta-Catenin staining was also observed around the cell sub-membrane. Transmission electron microscopy showed expended intercellular space. After 24 h, changes of HTM cells were recovered.


CONCLUSIONS
Hep II domains reversibly blocks actin cytoskeleton and cell-junction of HTM cells. Disorganization of actin cytoskeleton and cell-junction in trabecular meshwork through signal transduction may be a useful strategy for the decrease of intraocular pressure.
Zhang
Qing-Wei
QW
Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
Wang
Ning-Li
NL
Liu
Xu-Yang
XY
Chen
Feng-Hua
FH
Wang
Chun-Fang
CF
Ji
Ang
A
Liu
Xiao-Chao
XC
D000199:Actins / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D005353:Fibronectins / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007365:Intercellular Junctions / Q000187:drug effects; D010446:Peptide Fragments / Q000494:pharmacology; D014129:Trabecular Meshwork / Q000166:cytology* / Q000187:drug effects*
D000199:Actins; D005353:Fibronectins; D010446:Peptide Fragments; D006493:Heparin