Consistent left-right asymmetry cannot be established by late organizers in Xenopus unless the late organizer is a conjoined twin.
How embryos consistently orient asymmetries of the left-right (LR) axis is an intriguing question, as no macroscopic environmental cues reliably distinguish left from right. Especially unclear are the events coordinating LR patterning with the establishment of the dorsoventral (DV) axes and midline determination in early embryos. In frog embryos, consistent physiological and molecular asymmetries manifest by the second cell cleavage; however, models based on extracellular fluid flow at the node predict correct de novo asymmetry orientation during neurulation. We addressed these issues in Xenopus embryos by manipulating the timing and location of dorsal organizer induction: the primary dorsal organizer was ablated by UV irradiation, and a new organizer was induced at various locations, either early, by mechanical rotation, or late, by injection of lithium chloride (at 32 cells) or of the transcription factor XSiamois (which functions after mid-blastula transition). These embryos were then analyzed for the position of three asymmetric organs. Whereas organizers rescued before cleavage properly oriented the LR axis 90% of the time, organizers induced in any position at any time after the 32-cell stage exhibited randomized laterality. Late organizers were unable to correctly orient the LR axis even when placed back in their endogenous location. Strikingly, conjoined twins produced by late induction of ectopic organizers did have normal asymmetry. These data reveal that although correct LR orientation must occur no later than early cleavage stages in singleton embryos, a novel instructive influence from an early organizer can impose normal asymmetry upon late organizers in the same cell field.
Vandenberg
Laura N
LN
Center for Regenerative and Developmental Biology, and Biology Department, Tufts University, Medford, MA 02155, USA.
Levin
Michael
M
D000818:Animals; D019521:Body Patterning / Q000502:physiology*; D019070:Cell Lineage; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D004627:Embryonic Induction / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D055458:Left-Right Determination Factors / Q000378:metabolism; D018021:Lithium Chloride / Q000494:pharmacology; D020897:Organizers, Embryonic / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D012857:Situs Inversus / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D014428:Twins, Conjoined / Q000196:embryology*; D014466:Ultraviolet Rays; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis / Q000033:anatomy & histology / Q000196:embryology
D018398:Homeodomain Proteins; D055458:Left-Right Determination Factors; C093133:SIA1 protein, Xenopus; D029867:Xenopus Proteins; D018021:Lithium Chloride