title: Borrelia Burgdorferi "sensu lato" in Brazil: Occurrence confirmed by immunohistochemistry and focus floating microscopy.
abstract: In the present study, we report the occurrence of Lyme's borreliosis in patients from the Brazilian Amazon Region. Borreliosis was investigated by immunohistochemistry and focus floating microscopy for Borrelia burgdorferi in skin biopsy samples from 22 patients with both clinical and histopathology evidences compatible with Erythema Migrans. Spirochetes were detected by specific immunohistochemistry and focus floating microscopy for B. burgdorferi in samples from five patients. Clinical cure of the cutaneous lesions was observed in all the patients after treatment with doxycycline regimen as proposed by the Center Disease Control guidelines. A limitation of our study was the fact that we were not able to isolate and culture these organisms. These are the first known Brazilian cases of borreliosis to have Focus Floating Microscopy confirmation.
authors.affiliation: Fundação de Medicina Tropical do Amazonas, Manaus, Amazonas, Brazil.
authors.forename: Sinésio
authors.initials: S
authors.lastname: Talhari
authors.forename: Mônica Nunes
authors.initials: MN
authors.lastname: de Souza Santos
authors.forename: Carolina
authors.initials: C
authors.lastname: Talhari
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: de Lima Ferreira
authors.forename: Roberto Moreira
authors.initials: RM
authors.lastname: Silva
authors.forename: Bernhard
authors.initials: B
authors.lastname: Zelger
authors.forename: Cesare
authors.initials: C
authors.lastname: Massone
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Ribeiro-Rodrigues
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001706:Biopsy; D015748:Borrelia burgdorferi Group / Q000166:cytology / Q000302:isolation & purification*; D001938:Brazil / Q000453:epidemiology; D004318:Doxycycline / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008193:Lyme Disease / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D012867:Skin / Q000382:microbiology* / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D004318:Doxycycline