title: Effect of chlorhexidine application on long-term shear bond strength of resin cements to dentin.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effect of chlorhexidine [CH] on dentin bond strength of three resin cements after 1 year of water storage.
  
  
  METHODS
  A flat middle dentin surface was prepared on 120 extracted premolars. The teeth were randomly divided into 6 groups of 20 specimens each according to the resin cement used: Panavia F2.0, Variolink II, and RelyX Unicem, with or without CH application. After cementation of an indirect composite rod [Z250], one subgroup [n=10] was tested after 24h in water at 37 ° C and the other subgroup [n=10] was tested after 1 year storage in water plus thermocycling. A shear bond strength [SBS] test was performed. The data [in MPa] were analyzed with ANOVA and Tukey tests [P<0.05].
  
  
  RESULTS
  Three-way ANOVA [resin cement, CH and time] indicated that Variolink II had the highest strength [16.65 ± 3.60] and RelyX Unicem had the lowest strength [9.30 ± 4.07]. Chlorhexidine application increased SBS [13.31 ± 4.61] compared to samples without CH [12.16 ± 5.04] [P=0.04]. Initial SBS [15.63 ± 4.37] was significantly higher than after 1 year of storage [9.85 ± 3.36] [P<0.001]. Separate two-way ANOVA for 24-h and 1-year data showed that cement had a significant effect but CH and its interaction had no significant effect at 24h, whereas at 1 year the two factors and their interaction differed significantly [P ≤ 0.001].
  
  
  CONCLUSIONS
  Chlorhexidine 2% can diminish the loss of bonding effectiveness over time associated to etch-and-rinse and self-etch cements, although it appears not have any effect on self-adhesive cement.
authors.affiliation: Operative Dentistry Department, Dental Faculty, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Shafiei
authors.forename: Mahtab
authors.initials: M
authors.lastname: Memarpour
mesh_terms: D001641:Bicuspid; D002710:Chlorhexidine / Q000494:pharmacology*; D001840:Dental Bonding; D003804:Dentin / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D019279:Resin Cements; D033081:Shear Strength / Q000187:drug effects
chemical_list: D019279:Resin Cements; D002710:Chlorhexidine