Effects of biotic and abiotic elicitors on cell growth and tanshinone accumulation in Salvia miltiorrhiza cell cultures.
This study examined the effects of biotic and abiotic elicitors on the production of diterpenoid tanshinones in Salvia miltiorrhiza cell culture. Four classes of elicitors were tested, heavy metal ions (Co2+, Ag+, Cd2+), polysaccharides (yeast extract and chitosan), plant response-signaling compounds (salicylic acid and methyl jasmonate), and hyperosmotic stress (with sorbitol). Of these, Ag (silver nitrate), Cd (cadmium chloride), and polysaccharide from yeast extract (YE) were most effective to stimulate the tanshinone production, increasing the total tanshinone content of cell by more than ten-fold (2.3 mg g(-1) versus 0.2 mg g(-1) in control). The stimulating effect was concentration-dependent, most significant at 25 microM of Ag and Cd and 100 mg l(-1) (carbohydrate content) of YE. Of the three tanshinones detected, cryptotanshinone was stimulated most dramatically by about 30-fold and tanshinones I and IIA by no more than 5-fold. Meanwhile, most of the elicitors suppressed cell growth, decreasing the biomass yield by about 50% (5.1-5.5 g l(-1) versus 8.9 g l(-1) in control). The elicitors also stimulated the phenylalanine ammonia lyase activity of cells and transient increases in the medium pH and conductivity. The results suggest that the elicitor-stimulated tanshinone accumulation was a stress response of the cells.
Zhao
Jiang-Lin
JL
Department of Plant Pathology, China Agricultural University, Beijing 100193, China. lgzhou@cau.edu.cn
Zhou
Li-Gang
LG
Wu
Jian-Yong
JY
D045784:Abietanes; D000085:Acetates / Q000378:metabolism / Q000494:pharmacology; D018533:Biomass; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D019216:Metals, Heavy / Q000378:metabolism / Q000494:pharmacology; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology; D010616:Phenanthrenes / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism / Q000494:pharmacology; D027561:Salvia miltiorrhiza / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*
D045784:Abietanes; D000085:Acetates; D003470:Culture Media; D003517:Cyclopentanes; D019216:Metals, Heavy; D054883:Oxylipins; D010616:Phenanthrenes; C021751:tanshinone; C072239:methyl jasmonate; D020156:Salicylic Acid