Effect of interleukin-8 and RANTES on the Gardos channel activity in sickle human red blood cells: role of the Duffy antigen receptor for chemokines.
We investigated the effects of the chemokines IL-8 and RANTES on the activity of the Gardos channel (GC) of sickle red blood cells (SSRBCs). SSRBCs expressing the Duffy antigen receptor for chemokines (DARC) incubated under oxygenated conditions exhibit GC activation. The deoxygenation-stimulated K(+) loss via the GC is activated by the chemokines in the Duffy-positive SSRBCs. The percentage of cells with high density is 17 times higher in the Duffy-positive group. These findings are consistent with a greater susceptibility of Duffy-positive SSRBCs to inflammatory chemokines leading to GC activation and cellular dehydration and suggest a coupling, promoted by the sickling process, between DARC and the GC.
Durpès
Marie-Claude
MC
Inserm U763, Pointe-à-Pitre, F-97100, France; Université des Antilles et de la Guyane, F-97100 Guadeloupe, France.
Nebor
Danitza
D
du Mesnil
Pierre Couespel
PC
Mougenel
Danièle
D
Decastel
Monique
M
Elion
Jacques
J
Hardy-Dessources
Marie-Dominique
MD
D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood / Q000473:pathology*; D048430:Cell Shape; D018999:Charybdotoxin / Q000494:pharmacology; D018946:Chemokine CCL5 / Q000494:pharmacology*; D003681:Dehydration; D004375:Duffy Blood-Group System / Q000235:genetics* / Q000502:physiology; D004913:Erythrocytes, Abnormal / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels / Q000187:drug effects*; D017136:Ion Transport / Q000187:drug effects*; D010100:Oxygen / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000502:physiology*
C473745:ACKR1 protein, human; D018946:Chemokine CCL5; D004375:Duffy Blood-Group System; D016209:Interleukin-8; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; C496923:KCNN4 protein, human; D011956:Receptors, Cell Surface; D018999:Charybdotoxin; D011188:Potassium; D010100:Oxygen