title: Hsa-miR-34c suppresses growth and invasion of human laryngeal carcinoma cells via targeting c-Met.
abstract: MicroRNAs (miRNAs) are small noncoding RNA molecules that negatively modulate gene expression at the post-transcriptional level. A growing number of studies has shown that more and more miRNAs are aberrantly expressed and involved in the pathogenesis of several types of cancers. Here, we report that the down-regulated hsa-miR-34c was also involved in oncogenesis of laryngeal carcinoma. Our studies indicated that hsa-miR-34c functioned as a tumor suppressor which inhibited growth and invasion of human laryngeal carcinoma cells. Furthermore, in our study, an inverse relationship between the expression of hsa-miR-34c and c-Met was identified in 10 paired fresh samples from tumor tissues and adjacent normal tissues. Infection of hsa-miR-34c mediated by lentivirus suppressed the expression of c-Met directly. In addition, introduction of c-Met cDNA lacking 3'-UTR largely abrogated hsa-miR-34c-induced cell growth and invasion inhibition. These findings suggest aberrantly down-regulated hsa-miR-34c is a critical factor that contributes to malignancy in human laryngeal carcinoma by a mechanism involving targeting of c-Met.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Taizhou People's Hospital, Taizhou 225300, Jiangsu, P.R. China. njmucaikemin@126.com
authors.forename: Ke-Min
authors.initials: KM
authors.lastname: Cai
authors.forename: Xue-Li
authors.initials: XL
authors.lastname: Bao
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Kong
authors.forename: Wu
authors.initials: W
authors.lastname: Jinag
authors.forename: Ming-Rong
authors.initials: MR
authors.lastname: Mao
authors.forename: Jiu-Sheng
authors.initials: JS
authors.lastname: Chu
authors.forename: Yong-Jiu
authors.initials: YJ
authors.lastname: Huang
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Zhao
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017209:Apoptosis; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007822:Laryngeal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D018076:DNA, Complementary; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D019859:Proto-Oncogene Proteins c-met