title: Peptide-induced domain formation in supported lipid bilayers: direct evidence by combined atomic force and polarized total internal reflection fluorescence microscopy.
abstract: Direct visualization of the mechanism(s) by which peptides induce localized changes to the structure of membranes has high potential for enabling understanding of the structure-function relationship in antimicrobial and cell-penetrating peptides. We have applied a combined imaging strategy to track the interaction of a model antimicrobial peptide, PFWRIRIRR-amide, with bacterial membrane-mimetic supported phospholipid bilayers comprised of POPE/TOCL. Our in situ studies revealed rapid reorganization of the POPE/TOCL membrane into localized TOCL-rich domains with a concomitant change in the organization of the membranes themselves, as reflected by changes in fluorescent-membrane-probe order parameter, upon introduction of the peptide.
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Ontario, Canada.
authors.forename: John
authors.initials: J
authors.lastname: Oreopoulos
authors.forename: Raquel F
authors.initials: RF
authors.lastname: Epand
authors.forename: Richard M
authors.initials: RM
authors.lastname: Epand
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Yip
mesh_terms: D000042:Absorption; D000818:Animals; D002417:Cattle; D005456:Fluorescent Dyes / Q000737:chemistry; D008051:Lipid Bilayers / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D021962:Membrane Microdomains / Q000187:drug effects*; D018625:Microscopy, Atomic Force / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010455:Peptides / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008055:Lipids; D010455:Peptides