title: Triazophos up-regulated gene expression in the female brown planthopper, Nilaparvata lugens.
abstract: The widespread use of insecticides has caused the resurgence of the brown planthopper, Nilaparvata lugens, in Asia. In this study, we investigated an organo-phosphorous insecticide, triazophos, and its ability to induce gene expression variation in female N. lugens nymphs just before emergence. By using the suppression subtractive hybridization method, a triazophos-induced cDNA library was constructed. In total, 402 differentially expressed cDNA clones were obtained. Real-time qPCR analysis confirmed that triazophos up-regulated the expression of six candidate genes at the transcript level in nymphs on day 3 of the 5th instar. These genes encode N. lugens vitellogenin, bystin, multidrug resistance protein (MRP), purine nucleoside phosphorylase (PNP), pyrroline-5-carboxylate reductase (P5CR) and carboxylesterase. Our results imply that the up-regulation of these genes may be involved in the induction of N. lugens female reproduction or resistance to insecticides.
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Yan-Yuan
authors.initials: YY
authors.lastname: Bao
authors.forename: Bao-Ling
authors.initials: BL
authors.lastname: Li
authors.forename: Zhao-Bu
authors.initials: ZB
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Xue
authors.forename: Zeng-Rong
authors.initials: ZR
authors.lastname: Zhu
authors.forename: Jia-An
authors.initials: JA
authors.lastname: Cheng
authors.forename: Chuan-Xi
authors.initials: CX
authors.lastname: Zhang
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D015723:Gene Library; D006430:Hemiptera / Q000187:drug effects / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity*; D009758:Nymph / Q000187:drug effects / Q000378:metabolism; D063086:Organothiophosphates / Q000633:toxicity*; D016133:Polymerase Chain Reaction; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D011762:Pyrroline Carboxylate Reductases / Q000378:metabolism; D014230:Triazoles / Q000633:toxicity*; D014819:Vitellogenins / Q000378:metabolism
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D015815:Cell Adhesion Molecules; D017931:DNA Primers; D007306:Insecticides; D063086:Organothiophosphates; D014230:Triazoles; D014819:Vitellogenins; C012584:triazophos; D011762:Pyrroline Carboxylate Reductases; D011683:Purine-Nucleoside Phosphorylase