Analysis of nucleolar pre-rRNA processing sites in pea (Pisum sativum).
The location of rRNA processing was analyzed by using in situ hybridization with ITS1 probe and immunolabeling of anti-fibrillarin mAb in pea (Pisum sativum) root pole cells. The results showed that rRNA processing sites were in dense fibrillar components (DFCs) and granular components (GCs), but not in fibrillar centers (FCs). Low doses of actinomycin D (AMD) treatment can selectively suppress pre-rRNA synthesis but cannot disturb the processing of preformed pre-rRNAs. With AMD treatment prolonged, the density of labeled signals gradually decreased, indicating the preformed pre-rRNAs were gradually processed.
Long
Hong
H
Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
Zeng
Xianlu
X
Jiao
Mingda
M
Hu
Bo
B
Sun
Haijing
H
Liu
Zhenlan
Z
Zhang
Liyong
L
Hao
Shui
S
D001483:Base Sequence; D002466:Cell Nucleolus / Q000378:metabolism* / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D003609:Dactinomycin / Q000494:pharmacology; D017403:In Situ Hybridization; D016253:Microscopy, Immunoelectron; D018532:Pisum sativum / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D010940:Plant Proteins / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; D018744:DNA, Plant; D010940:Plant Proteins; D012322:RNA Precursors; D018749:RNA, Plant; C047458:fibrillarin; D003609:Dactinomycin