title: Transfection of siRNAs in multiple myeloma cell lines.
abstract: RNA interference (RNAi), a valuable tool to specifically silence gene expression, is becoming an indispensable weapon in the arsenal of functional genomics, target validation and gene-specific therapeutic research. We have shown that Streptolysin-O (SLO) reversible permeabilization is an efficient method to deliver small interfering RNAs (siRNAs) to hard-to-transfect human myeloma cell lines. In addition, we have shown that transfection of siRNAs, using the SLO reversible permeabilization, specifically and efficiently induces gene silencing in myeloma cell lines.
authors.affiliation: Section of Haemato-Oncology, Institute of Cancer Research, London, UK.
authors.forename: Jose L R
authors.initials: JL
authors.lastname: Brito
authors.forename: Nicola
authors.initials: N
authors.lastname: Brown
authors.forename: Gareth J
authors.initials: GJ
authors.lastname: Morgan
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D055785:Gene Knockdown Techniques; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D013301:Streptolysins / Q000378:metabolism; D014162:Transfection
chemical_list: D001426:Bacterial Proteins; D034741:RNA, Small Interfering; D013301:Streptolysins; C025964:streptolysin O