[Effects of silencing chloride intracellular channel 1 gene expression on the proliferation and invasion of mouse hepatocellular carcinoma cell lines].
OBJECTIVE
To study the effects of silencing CLIC1 gene expression on the proliferation and invasion of Hca-F cells.


METHODS
The mouse CLIC1 cDNA sequence was retrieved from NCBI. Three shRNA sequences were designed and cloned into pGPU6/GFP/Neo plasmids. The plasmids were transfected into Hca-F cells with Lipofectamine 2000. Cell Counting-8 (CCK-8) kit and transwell chamber were used to study the effects of CLIC1 on the proliferation and invasion of Hca-F cells.


RESULTS
The pGPU6/GFP/Neo-shRNA-3 plasmid effectively repressed the expression of CLIC1 mRNA. Inhibition of CLIC1 gene expression led to decreased cell proliferation and reduced invasion.


CONCLUSIONS
CLIC1 is essential for the proliferation and invasion of Hca-F cells.
Li
Rong-kuan
RK
Department of Gastroenterology and Hepatology, the Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China.
Tang
Jian-wu
JW
Zhang
Jun
J
Wang
Shao-qing
SQ
Wang
Mei
M
Wang
Bo
B
Zhang
Yu-hong
YH
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D009361:Neoplasm Invasiveness; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D018118:Chloride Channels; C501967:Clic1 protein, mouse; D012333:RNA, Messenger; D034741:RNA, Small Interfering