title: Pretreatment with oxygen protects rat kidney from cisplatin nephrotoxicity.
abstract: Cisplatin (CP) nephrotoxicity is mainly due to reactive oxygen species. Oxygen pre-exposure as a mild oxidative stress may enhance some endogenous defense mechanisms, so its effect on cisplatin-induced acute renal failure was investigated in present study. Twenty-four rats were divided into four groups. The O(2)+ CP and Air + CP groups were were subjected to i.p. injection of 5 mg/kg cisplatin, and in the Air + Saline and O(2) + Saline groups, saline was injected instead of cisplatin. O(2)+ CP and O(2)+ Saline groups were pretreated with oxygen (3h/d for two days), and the other two groups were pretreated with room air. Cisplatin was administered 24 h after last pretreatment session. Three days after cisplatin injection, plasma samples were obtained, and parts of kidney tissue were frozen for biochemical analysis or fixed in formalin for histological assessments. Preconditioning with oxygen prior to cisplatin administration led to reduced tubular necrosis and luminal cast formation and improvement of renal function, as was evidenced by significant reduction in plasma creatinine and urea levels. Oxygen pretreatment also significantly reversed cisplatin-induced reduction in renal catalase activity and glutathione level. It could be concluded that oxygen pretreatment could have a delayed protective effect against cisplatin nephrotoxicity, and that increased renal catalase activity may be involved in this protective effect of hyperoxia.
authors.affiliation: Razi Herbal Medicines Research Center and Department of Physiology, Lorestan University of Medical Sciences, Khorramabad, Iran. bahramrasoulian@gmail.com
authors.forename: Bahram
authors.initials: B
authors.lastname: Rasoulian
authors.forename: Mahvash
authors.initials: M
authors.lastname: Jafari
authors.forename: Mirgholamreza
authors.initials: M
authors.lastname: Mahbod
authors.forename: Mansour Esmaili
authors.initials: ME
authors.lastname: Dehaj
authors.forename: Majid
authors.initials: M
authors.lastname: Nowrozi
authors.forename: Hannaneh
authors.initials: H
authors.lastname: Wahhabaghai
authors.forename: Mahmood
authors.initials: M
authors.lastname: Mofid
authors.forename: Asghar
authors.initials: A
authors.lastname: Ghasemi
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Bigdeli
authors.forename: Ali
authors.initials: A
authors.lastname: Khoshbaten
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002945:Cisplatin / Q000633:toxicity*; D007677:Kidney Function Tests; D008274:Magnesium / Q000097:blood; D008297:Male; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D008274:Magnesium; D002945:Cisplatin; D010100:Oxygen