title: Determination of chloramphenicol residues in milk by enzyme-linked immunosorbent assay: improvement by biotin-streptavidin-amplified system.
abstract: A sensitive biotin-streptavidin amplified enzyme-linked immunosorbent assay (BA-ELISA) method was developed for the determination of chloramphenicol residues in milk. The biotin-streptavidin system was applied to enhance the sensitivity. After optimization, the detection limit of the method was found to be 0.042 +/- 0.006 ng mL(-1), which is 8-fold more sensitive than the traditional competitive ELISA using the same antibody and coating antigen. The amplification mechanism of the biotin-streptavidin system and the major factors affecting the sensitivity of detection are discussed. This method was successfully applied to determine the chloramphenicol residues in milk samples with a simple and rapid extraction procedure, and good recoveries (85.66-109.67%) were obtained. The result indicated that the biotin-streptavidin system may be a valuable tool to improve the specific detection of trace veterinary drug residues and could be widely used for routine monitoring of food samples.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Zhenjuan
authors.initials: Z
authors.lastname: Duan
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D001710:Biotin / Q000032:analysis; D002701:Chloramphenicol / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D019809:Streptavidin / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D002701:Chloramphenicol; D001710:Biotin; D019809:Streptavidin