title: C/EBPα down-regulation is associated with reduced hepatic cellular viability during hypoxia in vitro and in vivo.
abstract: C/EBPα transcription factor is a key regulator in liver biology and was preliminarily shown to be down-regulated in hypoxic primary rat hepatocytes. The aim of this study was to explore the possible association between C/EBPα expression level and hepatocyte viability in both the in-vitro cultured hypoxic rat primary hepatocytes and two models of acute liver hypoxia induced by carbon tetrachloride or Fas antibody. C/EBPα mRNA was significantly down-regulated under hypoxic conditions both in vitro and in vivo, which was paralleled by a similar decrease in hepatocyte viability and partially reversed by 3D matrix and dexamethasone. These results suggested that C/EBPα down-regulation may be one mechanism of reduced hepatocyte viability in these settings.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital, Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing, 400016, PR China.
authors.forename: Qian Yun
authors.initials: QY
authors.lastname: Zhou
authors.forename: Ding
authors.initials: D
authors.lastname: Liu
authors.forename: Shi Feng
authors.initials: SF
authors.lastname: Huang
authors.forename: Yang An
authors.initials: YA
authors.lastname: Wen
authors.forename: Peng
authors.initials: P
authors.lastname: Luo
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiang
authors.forename: Shan
authors.initials: S
authors.lastname: Sun
authors.forename: Yu Fang
authors.initials: YF
authors.lastname: Dong
authors.forename: Li Ping
authors.initials: LP
authors.lastname: Zhang
mesh_terms: D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism*; D015687:Cell Hypoxia; D002470:Cell Survival; D015536:Down-Regulation; D022781:Hepatocytes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D012333:RNA, Messenger