title: An antibody profile of systemic lupus erythematosus detected by antigen microarray.
abstract: Patients with systemic lupus erythematosus (SLE) produce antibodies to many different self-antigens. Here, we investigated antibodies in SLE sera using an antigen microarray containing many hundreds of antigens, mostly self-antigens. The aim was to detect sets of antibody reactivities characteristic of SLE patients in each of various clinical states--SLE patients with acute lupus nephritis, SLE patients in renal remission, and SLE patients who had never had renal involvement. The analysis produced two novel findings: (i) an SLE antibody profile persists independently of disease activity and despite long-term clinical remission, and (ii) this SLE antibody profile includes increases in four specific immunoglobulin G (IgG) reactivities to double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), Epstein-Barr virus (EBV) and hyaluronic acid; the profile also includes decreases in specific IgM reactivities to myeloperoxidase (MPO), CD99, collagen III, insulin-like growth factor binding protein 1 (IGFBP1) and cardiolipin. The reactivities together showed high sensitivity (> 93%) and high specificity for SLE (> 88%). A healthy control subject who had the SLE antibody profile was later found to develop clinical SLE. The present study did not detect antibody reactivities that differentiated among the various subgroups of SLE subjects with statistical significance. Thus, SLE is characterized by an enduring antibody profile irrespective of clinical state. The association of SLE with decreased IgM natural autoantibodies suggests that these autoantibodies might enhance resistance to SLE.
authors.affiliation: Department of Nephrology, Rabin Medical Center, Petach Tikva, Israel.
authors.forename: Ittai
authors.initials: I
authors.lastname: Fattal
authors.forename: Noam
authors.initials: N
authors.lastname: Shental
authors.forename: Dror
authors.initials: D
authors.lastname: Mevorach
authors.forename: Juan-Manuel
authors.initials: JM
authors.lastname: Anaya
authors.forename: Avi
authors.initials: A
authors.lastname: Livneh
authors.forename: Pnina
authors.initials: P
authors.lastname: Langevitz
authors.forename: Gisele
authors.initials: G
authors.lastname: Zandman-Goddard
authors.forename: Rachel
authors.initials: R
authors.lastname: Pauzner
authors.forename: Miriam
authors.initials: M
authors.lastname: Lerner
authors.forename: Miri
authors.initials: M
authors.lastname: Blank
authors.forename: Maria-Eugenia
authors.initials: ME
authors.lastname: Hincapie
authors.forename: Uzi
authors.initials: U
authors.lastname: Gafter
authors.forename: Yaakov
authors.initials: Y
authors.lastname: Naparstek
authors.forename: Yehuda
authors.initials: Y
authors.lastname: Shoenfeld
authors.forename: Eytan
authors.initials: E
authors.lastname: Domany
authors.forename: Irun R
authors.initials: IR
authors.lastname: Cohen
mesh_terms: D000071443:12E7 Antigen; D000328:Adult; D017153:Antibodies, Anticardiolipin / Q000276:immunology; D000974:Antibodies, Antinuclear / Q000097:blood / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology; D024061:Collagen Type III / Q000276:immunology; D015536:Down-Regulation / Q000276:immunology; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D006820:Hyaluronic Acid / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000276:immunology*; D008181:Lupus Nephritis / Q000175:diagnosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009195:Peroxidase / Q000276:immunology; D040081:Protein Array Analysis; D012680:Sensitivity and Specificity; D015854:Up-Regulation / Q000276:immunology
chemical_list: D000071443:12E7 Antigen; D017153:Antibodies, Anticardiolipin; D000974:Antibodies, Antinuclear; D015703:Antigens, CD; D001323:Autoantibodies; C061828:CD99 protein, human; D015815:Cell Adhesion Molecules; D024061:Collagen Type III; C032231:IGFBP1 protein, human; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D018970:Insulin-Like Growth Factor Binding Protein 1; D006820:Hyaluronic Acid; D009195:Peroxidase