Potentiation of bacterial killing activity of zinc chloride by pyrrolidine dithiocarbamate.
Zinc has antimicrobial activity and zinc salts including zinc chloride (ZnCl(2)) have been used for the control of oral malodor. In this study, we hypothesized that pyrrolidine dithiocarbamate (PDTC), a zinc ionophore, may enhance antimicrobial efficacy of ZnCl(2). The bactericidal effectiveness of ZnCl(2) alone (0.5-8 mM) or in combination with PDTC (1 or 10 microM) was evaluated by in vitro short (1 h) time-killing assays against Fusobacterium nucleatum and Porphyromonas gingivalis. Only a slight viability decrease was observed with ZnCl(2) or PDTC alone after 1-h incubation. By contrast, combination of ZnCl(2) and PDTC could achieve a more than 100-fold viability reduction compared with ZnCl(2) or PDTC alone in F. nucleatum and P. gingivalis. Therefore, PDTC greatly enhanced the bactericidal activity of ZnCl(2) against the oral malodor-producing bacteria. These results suggest that use of PDTC may be useful for enhancing bactericidal activity of antimalodor regimens of zinc salts.
Choi
Eun-Kyoung
EK
Brain Korea 21 Program, Chonnam National University Dental School, Gwangju, Republic of Korea.
Lee
Hye-Hyang
HH
Kang
Mi-Sun
MS
Kim
Byung-Gook
BG
Lim
Hoi-Soon
HS
Kim
Seon-Mi
SM
Kang
In-Chol
IC
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002712:Chlorides / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004357:Drug Synergism; D016967:Fusobacterium nucleatum / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000254:growth & development; D011759:Pyrrolidines / Q000494:pharmacology*; D013859:Thiocarbamates / Q000494:pharmacology*; D017967:Zinc Compounds / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D002712:Chlorides; D011759:Pyrrolidines; D013859:Thiocarbamates; D017967:Zinc Compounds; C020972:pyrrolidine dithiocarbamic acid; C016837:zinc chloride