Hesperidin attenuates mitochondrial dysfunction during benzo(a)pyrene-induced lung carcinogenesis in mice.
The present study is designed to assess the mitochondrial status during benzo(a)pyrene (B(a)P)-induced lung carcinogenesis in Swiss albino mice and to reveal the modulatory effect of hesperidin over it. B(a)P (50 mg/kg body weight)-induced mitochondrial abnormalities was evident from alterations in mitochondrial lipid peroxides, antioxidant status (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, reduced glutathione, vitamin E, and vitamin C), major tricarboxylic acid (TCA) cycle enzyme activities (isocitrate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, alpha-ketoglutarate dehydrogenase), electron transport chain (ETC) complexes activities and ATP levels. Ultrastructural changes in lung mitochondria were also in accord with the above aberrations. Hesperidin (25 mg/kg body weight) supplementation effectively counteracted all the above changes and restored cellular normalcy, indicating its protective role during B(a)P-induced lung cancer.
Kamaraj
Sattu
S
Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600 025, India.
Anandakumar
Pandi
P
Jagan
Sundaram
S
Ramakrishnan
Gopalakrishnan
G
Devaki
Thiruvengadam
T
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D001564:Benzo(a)pyrene / Q000633:toxicity; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism; D006569:Hesperidin / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008175:Lung Neoplasms / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000473:pathology / Q000517:prevention & control
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D045222:Electron Transport Chain Complex Proteins; D001564:Benzo(a)pyrene; D000255:Adenosine Triphosphate; D006569:Hesperidin