title: Effects of vitamin E on the osteoblast differentiation.
abstract: Vitamin E is thought to affect bone formation and bone remodeling. In this study, we investigated the effects of vitamin E (alpha-tocopherol and delta-tocopherol) on the osteoblasts isolated from rat calvariae. At 4 and 7 days (Day 4 and 7) after induction of osteoblastic differentiation, treatment of alpha-tocopherol (100 and 200 microM) and delta-tocopherol (2 and 20 microM) for 3 days significantly decreased alkaline phophatase activity of the cultured osteoblasts. At Day 14, however, no significant change was detected in ALP activity and expression of bone sialoprotein mRNA in the osteoblasts treated with alpha-tocopherol or delta-tocopherol for 3 days. Expression of osteocalcin mRNA was decreased by treatment of alpha-tocopherol (100 and 200 microM) and delta-tocopherol (2 and 20 microM) at Day 4 and 7. At Day 14, expression of osteocalcin mRNA was decreased only with treatment of 200 microM alpha-tocopherol. In addition, the noncalcified nodules were decreased by treatment of alpha-tocopherol (200 microM) and delta-tocopherol (20 microM) at Day 7. However, treatment of alpha-tocopherol and delta-tocopherol showed no significant change of formation of calcified nodules at Day 14. These results indicate that vitamin E inhibits differentiation of osteoblasts especially from early stage to osteoid-producing stage.
authors.affiliation: Department of Veterinary Anatomy, Nippon Veterinary and Animal Science University, Japan. ssoeta@wta.att.ne.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Soeta
authors.forename: Masaya
authors.initials: M
authors.lastname: Higuchi
authors.forename: Itaru
authors.initials: I
authors.lastname: Yoshimura
authors.forename: Ryu
authors.initials: R
authors.lastname: Itoh
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Kimura
authors.forename: Hajime
authors.initials: H
authors.lastname: Aamsaki
mesh_terms: D000818:Animals; D002114:Calcinosis / Q000473:pathology / Q000662:veterinary; D002454:Cell Differentiation / Q000187:drug effects; D017931:DNA Primers; D007536:Isomerism; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D015675:Osteocalcin / Q000235:genetics; D051381:Rats; D012886:Skull / Q000166:cytology; D014810:Vitamin E / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D015675:Osteocalcin; D014810:Vitamin E; D024502:alpha-Tocopherol