A refined map of early gene expression in the dorsal rhombomere 1 of mouse embryos.
Rhombomere 1 (r1), a temporary structure in the early developing brain, is bounded rostrally by the isthmus organizer and caudally by the r2 domain. Many genes involved in r1 induction and patterning have been identified. However, our knowledge of a comprehensive map of r1 regionalization defined by these genes at early embryonic stages remains fragmentary. In the present study, we examined the expression of a variety of genes in the dorsal r1 of E9.0-E10.5 mouse embryos. The expression domains within ventricular zone of these genes examined allowed us to define four distinct regions along the anterior-posterior (A-P) axis of the dorsal r1: the Wnt1/Lmx1a/Gdf7/Msx1/Msx2-positive roof plate, Math1/Olig3/Msx1/Msx2-positive, upper rhombic lip, Mash1/Ngn1/Ngn2-positive intermediate domain, and Mash1/Ngn1/Ngn2-positive rostral domain. Moreover, we defined the distribution of several genes expressed in the mantle zone of the dorsal r1, including Lmx1b, Lhx2, Lhx9, Phox2a and Phox2b. Taken together, our gene expression data identify a refined subdivision of the dorsal r1 with four distinct domains along the A-P axis and a mantle zone at early embryonic stages.
Liu
Zhi-Rong
ZR
Department of Neurology, Xijng Hospital, Fourth Military Medical University, 15 Changle-xi Road, Xi'an, Shaanxi 710032, China.
Shi
Ming
M
Hu
Ze-Lan
ZL
Zheng
Min-Hua
MH
Du
Fang
F
Zhao
Gang
G
Ding
Yu-Qiang
YQ
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000033:anatomy & histology / Q000196:embryology* / Q000502:physiology; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D017403:In Situ Hybridization; D060850:LIM-Homeodomain Proteins; D051957:MSX1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009024:Morphogenesis; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D051155:Wnt1 Protein / Q000235:genetics / Q000378:metabolism
D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; D051957:MSX1 Transcription Factor; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; D051155:Wnt1 Protein; C499317:Ascl1 protein, mouse; C500943:Atoh1 protein, mouse; C489692:Lmx1a protein, mouse; C084119:MSX2 protein; C484084:Msx1 protein, mouse; C102938:Neurog2 protein, mouse; C420992:Olig3 protein, mouse; C495246:Wnt1 protein, mouse; C062602:transcription factor PTF1; C486336:Neurog1 protein, mouse