Oncostatin M induces dendritic cell maturation and Th1 polarization.
Oncostatin M (OSM) is a pleiotropic cytokine and a member of the gp130/IL-6 cytokine family that has been found to be involved in both pro- and anti-inflammatory responses in cell-mediated immunity. Maturation of dendritic cells (DCs) is crucial for initiation of primary immune responses and is regulated by several stimuli. In this study, the role of OSM in the phenotypic and functional maturation of DCs was evaluated in vitro. Stimulation with OSM upregulated the expression of CD80, CD86, MHC class I and MHC class II and reduced the endocytic capacity of immature DCs. Moreover, OSM induced the allogeneic immunostimulatory capacity of DCs by stimulating the production of the Th1-promoting cytokine IL-12. OSM also increased the production of IFN-gamma by T cells in mixed-lymphocyte reactions, which would be expected to contribute to the Th1 polarization of the immune response. The expression of surface markers and cytokine production in DCs was mediated by both the MAPK and NF-kappaB pathways. Taken together, these results indicate that OSM may play a role in innate immunity and in acquired immunity by enhancing DCs maturation and promoting Th1 immune responses.
Jung
In Duk
ID
Department of Microbiology and Immunology and National Research Laboratory of Dendritic Cell Differentiation and Regulation, School of Medicine, Pusan National University, Beom-eo Ri, Mulgum Eop, Yangsan, Gyeongsangnam-do 626-770, South Korea.
Noh
Kyung Tae
KT
Lee
Chang-Min
CM
Chun
Sung Hak
SH
Jeong
Soo Kyung
SK
Park
Jin Wook
JW
Park
Won Sun
WS
Kim
Han Wool
HW
Yun
Cheol-Heui
CH
Shin
Yong Kyoo
YK
Park
Yeong-Min
YM
D021581:Active Transport, Cell Nucleus; D056704:Adaptive Immunity; D000818:Animals; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D004789:Enzyme Activation; D007113:Immunity, Innate; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D020929:Mitogen-Activated Protein Kinase Kinases / Q000096:biosynthesis; D016328:NF-kappa B / Q000378:metabolism; D053683:Oncostatin M / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D053681:Oncostatin M Receptor beta Subunit / Q000096:biosynthesis; D018417:Th1 Cells / Q000276:immunology*
D016328:NF-kappa B; D053681:Oncostatin M Receptor beta Subunit; D053683:Oncostatin M; D016753:Interleukin-10; D018664:Interleukin-12; D020929:Mitogen-Activated Protein Kinase Kinases