title: Genetic variation in the indoleamine 2,3-dioxygenase gene in pre-eclampsia.
abstract: OBJECTIVE
  To investigate the contribution of genomic variations in the indoleamine 2,3-dioxygenase (IDO) gene to the onset of pre-eclampsia.
  
  
  METHODS
  We examined sequence variations in the IDO1 gene using placental genomic DNA from 35 pre-eclamptic patients and 32 normotensive pregnant women.
  
  
  RESULTS
  A case-control study revealed that none of the common variants influences the risk of disease. Sequencing of each IDO1 exon in diseased subjects revealed rare variants. This variation, c.-147_150delGAAA, was located within the 5'-untranslated region of the IDO1 gene, and its homozygote was identified only in pre-eclamptic subjects. However, despite the low levels of IDO expression and enzyme activity in the c.-147_150delGAAA homozygote, reporter assays indicated that this variation does not affect gene expression.
  
  
  CONCLUSIONS
  Our findings indicate that genetic alteration of fetal IDO gene does not appear to be a primary cause of pre-eclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Haruki
authors.initials: H
authors.lastname: Nishizawa
authors.forename: Takema
authors.initials: T
authors.lastname: Kato
authors.forename: Sayuri
authors.initials: S
authors.lastname: Ota
authors.forename: Sachie
authors.initials: S
authors.lastname: Nishiyama
authors.forename: Kanako
authors.initials: K
authors.lastname: Pryor-Koishi
authors.forename: Machiko
authors.initials: M
authors.lastname: Suzuki
authors.forename: Makiko
authors.initials: M
authors.lastname: Tsutsumi
authors.forename: Hidehito
authors.initials: H
authors.lastname: Inagaki
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kurahashi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Udagawa
mesh_terms: D000328:Adult; D000818:Animals; D016022:Case-Control Studies; D005260:Female; D014644:Genetic Variation; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics*; D051379:Mice; D010920:Placenta / Q000378:metabolism; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase