title: [Effects of traditional Chinese drugs Shuganyishen, on Ishikawa cell line].
abstract: OBJECTIVE
  To study the effects of the traditional Chinese drugs, Shuganyishen, on Ishikawa cell line.
  
  
  METHODS
  Rat blood serum was prepared by using Chinese drugs serum pharmacology method, in which, Shuganyishen was contained or not contained. Ishikawa cells were subjected into five groups: (1) normal cells group (NCG), (2) rat blood serum without Shuganyishen group (NSGG) as negative control, (3) Shuganyishen group (SGG), (4) Cisplatin group (DDPG) as positive control, and (5) Shuganyishen with Cisplatin group (S&CG). MTT assay was used to evaluate the inhibition effect on cell growth in each group. The mRNA expression of estrogen receptor alpha and beta (ERalpha and ERbeta) in each group was detected by RT-PCR.
  
  
  RESULTS
  Effective inhibition of cell growth was found in the groups of SGG and S&CG, respectively (P < 0.05), which had no significant difference from positive control by MTT. The mRNA expression of ERbeta was slightly going up, while the expression of ERalpha was hardly effected in SGG by RT-PCR. Interestingly in S&CG, the mRNA expression of ERalpha was significantly down-regulated (P < 0.05), but the mRNA expression of ERbeta remained no change. As the positive control, the mRNA expression of ERalpha and ERbeta was significantly down-regulated in DDPG (P < 0.5).
  
  
  CONCLUSIONS
  Traditional Chinese drugs, Shuganyishen, may inhibit Ishikawa cell growth and had no effects on the expression of ERalpha, furthermore, can reverse the inhibition of ERbeta expression by Cisplatin.
authors.affiliation: The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China. fanxiuhua6666@163.com
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Fan
authors.forename: Wenping
authors.initials: W
authors.lastname: Lu
authors.forename: Han
authors.initials: H
authors.lastname: Wei
authors.forename: Xiangying
authors.initials: X
authors.lastname: Li
authors.forename: Jianhui
authors.initials: J
authors.lastname: Cai
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016889:Endometrial Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000941:ethics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D011960:Receptors, Estrogen