title: Taxonomic and functional diversity of atrazine-degrading bacterial communities enriched from agrochemical factory soil.
abstract: OBJECTIVE
  To characterize atrazine-degrading potential of bacterial communities enriched from agrochemical factory soil by analysing diversity and organization of catabolic genes.
  
  
  RESULTS
  The bacterial communities enriched from three different sites of varying atrazine contamination mineralized 65-80% of (14) C ring-labelled atrazine. The presence of trzN-atzBC-trzD, trzN-atzABC-trzD and trzN-atzABCDEF-trzD gene combinations was determined by PCR. In all enriched communities, trzN-atzBC genes were located on a 165-kb plasmid, while atzBC or atzC genes were located on separated plasmids. Quantitative PCR revealed that catabolic genes were present in up to 4% of the community. Restriction analysis of 16S rDNA clone libraries of the three enrichments revealed marked differences in microbial community structure and diversity. Sequencing of selected clones identified members belonging to Proteobacteria (α-, β- and γ-subclasses), the Actinobacteria, Bacteroidetes and TM7 division. Several 16S rRNA gene sequences were closely related to atrazine-degrading community members previously isolated from the same contaminated site.
  
  
  CONCLUSIONS
  The enriched communities represent a complex and diverse bacterial associations displaying heterogeneity of catabolic genes and their functional redundancies at the first steps of the upper and lower atrazine-catabolic pathway. The presence of catabolic genes in small proportion suggests that only a subset of the community has the capacity to catabolize atrazine.
  
  
  CONCLUSIONS
  This study provides insights into the genetic specificity and the repertoire of catabolic genes within bacterial communities originating from soils exposed to long-term contamination by s-triazine compounds.
authors.affiliation: Rudjer Bošković Institute, Center for Marine and Environmental Research, Zagreb, Croatia. nudikov@irb.hr
authors.forename: N
authors.initials: N
authors.lastname: Udiković-Kolić
authors.forename: D
authors.initials: D
authors.lastname: Hršak
authors.forename: M
authors.initials: M
authors.lastname: Devers
authors.forename: V
authors.initials: V
authors.lastname: Klepac-Ceraj
authors.forename: I
authors.initials: I
authors.lastname: Petrić
authors.forename: F
authors.initials: F
authors.lastname: Martin-Laurent
mesh_terms: D001280:Atrazine / Q000378:metabolism*; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D015723:Gene Library; D005798:Genes, Bacterial; D006540:Herbicides / Q000378:metabolism; D010802:Phylogeny; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D006540:Herbicides; D012987:Soil; D012989:Soil Pollutants; D001280:Atrazine