High-resolution tomographic diffractive microscopy of biological samples.
The authors have developed a tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens, with high lateral resolution. The authors now study its use for biological applications, and imaged several preparations with fluorescence confocal microscopy and tomographic diffractive microscopy. The results highlight some interesting features of this instrument, which should attract the interest of biologists for this new technique.
Simon
Bertrand
B
Laboratoire MIPS-EA2332, Université de Haute-Alsace, IUT Mulhouse, Mulhouse, France.
Debailleul
Matthieu
M
Beghin
Anne
A
Tourneur
Yves
Y
Haeberlé
Olivier
O
D000042:Absorption; D002138:Calibration; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000737:chemistry / Q000821:virology; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000166:cytology; D006696:Holography / Q000379:methods*; D006801:Humans; D021621:Imaging, Three-Dimensional; D053122:Influenza A Virus, H3N2 Subtype; D008853:Microscopy / Q000379:methods*; D009055:Mouth / Q000166:cytology; D014054:Tomography / Q000379:methods*