[Production of interleukin-8 by circulating CLA+ T cells with skin tropism in patients with psoriasis and in healthy controls].
BACKGROUND
Psoriasis is an immune-mediated disease typically associated with cutaneous neutrophilic infiltration and Munro microabscesses. Interleukin (IL)-8 is one of the main neutrophil-attracting chemokines. Although keratinocytes have traditionally been considered to be the principal source of IL-8 in psoriasis, we present data that suggest that cutaneous lymphocyte associated antigen (CLA) + T lymphocytes synthesize this cytokine.


METHODS
Six patients with psoriasis and 6 healthy controls were studied. Immunomagnetic separation was used to isolate CLA+ and CLA- T lymphocytes and IL-8 and interferon (IFN)-gamma production was quantified for each cell subpopulation using enzyme-linked immunosorbent assay. Finally, gene expression of IL-8 was analyzed by reverse transcriptase-polymerase chain reaction.


RESULTS
CLA+ and CLA- T lymphocytes from patients with psoriasis and from controls showed a significantly increased production of IFN-gamma when activated, whereas only activated CLA+ T lymphocytes (from patients and controls) synthesized IL-8. The higher level of expression of IL-8 and IFN-gamma by CLA+T lymphocytes in comparison to CLA- cells was confirmed.


CONCLUSIONS
Previous studies have confirmed IL-8 production by T lymphocytes in inflammatory skin diseases with neutrophil-rich infiltrates, such as acute generalized exanthematous pustulosis, Behçet disease, and pustular psoriasis. We have confirmed the role of the subset of T lymphocytes with skin tropism (CLA+) in IL-8 production in nonpustular psoriasis.
Ferran
M
M
Servicio de Dermatología, Hospital del Mar-IMIM, Barcelona, Spain. mferran@imas.imim.es
Ferran
M
M
Galván
A B
AB
Giménez-Arnau
A
A
Giménez-Arnau
A
A
Pujol
R M
RM
Pujol
R M
RM
Santamaría-Babi
L F
LF
Santamaría-Babi
L F
LF
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002633:Chemotaxis; D003199:Computer Systems; D005786:Gene Expression Regulation; D006801:Humans; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000032:analysis*; D009504:Neutrophils / Q000276:immunology / Q000502:physiology; D011565:Psoriasis / Q000097:blood / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology
D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; C063419:CTAGE1 protein, human; D016209:Interleukin-8; D008562:Membrane Glycoproteins; D007371:Interferon-gamma