title: Assembly of a large Y-STR haplotype database for the Czech population and investigation of its substructure.
abstract: Twelve Y-chromosomal short tandem repeats (Y-STR) (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a, DYS385b, DYS437, DYS438, and DYS439) included in the PowerPlex Y Kit (Promega Corporation, Madison, USA) were studied for 1750 unrelated males living in 14 regions of the Czech Republic. A total of 1148 different haplotypes were found. The overall haplotype diversity (HD) was determined as 0.998. Analysis of Molecular Variance (AMOVA) reveals non-significant distances between regions concerning their haplotype distribution, thus allowing to use the whole sample as a representative reference database of the Czech Republic. Median network analysis shows a remarkable bipartite composition of the Czech haplotypes, falling in distinct clusters with Eastern and Western European roots.
authors.affiliation: Laboratory for Molecular Genetics and Oncology, Genomac International, Ltd., Prague, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Zastera
authors.forename: Lutz
authors.initials: L
authors.lastname: Roewer
authors.forename: Sascha
authors.initials: S
authors.lastname: Willuweit
authors.forename: Patrik
authors.initials: P
authors.lastname: Sekerka
authors.forename: Lucie
authors.initials: L
authors.lastname: Benesova
authors.forename: Marek
authors.initials: M
authors.lastname: Minarik
mesh_terms: D041322:Chromosomes, Human, Y; D018153:Czech Republic; D016172:DNA Fingerprinting; D030561:Databases, Nucleic Acid; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences