Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats.
More sensitive methodologies are necessary to improve strongyloidiasis diagnosis. This study compared the sensitivities of the McMaster modified technique and polymerase chain reaction (PCR) assays, both performed in faecal samples. Lewis rats were subcutaneously infected with 4,000, 400 or 40 infective third-stage larvae, considered as high, moderate or low infection, respectively. Seven days later, they were euthanized to count adult nematodes recovered from the small intestine. Stool samples were used to count the number of eggs per gram (EPG) of faeces and to detect parasite DNA by PCR performed with a species and a genus primer pair. The sensitivity of these assays depended upon parasite burden and the primer specificity. All assays presented 100% sensitivity at the highest parasite load. In the moderate infection, EPG and PCR with the genus primer maintained 100% specificity, whereas PCR sensitivity with the species primer decreased to 77.7%. In low infection, the sensitivity was 60% for EPG, 0% for PCR with the species primer and 90% for PCR done with the genus primer. Together, these results suggest that PCR with a genus primer can be a very sensitive methodology to detect Strongyloides venezuelensisin faeces of Lewis rats infected with very low parasite burden.
Marra
Nelson Mendes
NM
Departamento de Microbiologia e Imunologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brasil, 18618-000.
Chiuso-Minicucci
Fernanda
F
Machado
Gabriel Capella
GC
Zorzella-Pezavento
Sofia Fernanda Gonçalves
SF
França
Thaís Graziela Donegá
TG
Ishikawa
Larissa Lumi Watanabe
LL
Amarante
Alessandro F T
AF
Sartori
Alexandrina
A
Amarante
Mônica R V
MR
D000818:Animals; D018509:DNA, Helminth / Q000032:analysis; D005243:Feces / Q000469:parasitology*; D005260:Female; D005838:Genotype; D008297:Male; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction; D051381:Rats; D011917:Rats, Inbred Lew; D012680:Sensitivity and Specificity; D013321:Strongyloides / Q000235:genetics / Q000302:isolation & purification; D013322:Strongyloidiasis / Q000175:diagnosis*
D018509:DNA, Helminth