title: Implication of the C terminus of the Prunus necrotic ringspot virus movement protein in cell-to-cell transport and in its interaction with the coat protein.
abstract: The movement protein (MP) of Prunus necrotic ringspot virus (PNRSV) is required for viral transport. Previous analysis with MPs of other members of the family Bromoviridae has shown that the C-terminal part of these MPs plays a critical role in the interaction with the cognate coat protein (CP) and in cell-to-cell transport. Bimolecular fluorescence complementation and overlay analysis confirm an interaction between the C-terminal 38 aa of PNRSV MP and its cognate CP. Mutational analysis of the C-terminal region of the PNRSV MP revealed that its C-terminal 38 aa are dispensable for virus transport, however, the 4 aa preceding the dispensable C terminus are necessary to target the MP to the plasmodesmata and for the functionality of the protein. The capacity of the PNRSV MP to use either a CP-dependent or a CP-independent cell-to-cell transport is discussed.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, IBMCP (UPV-CSIC), Avenida Ingeniero Fausto Elio s/n, 46022 Valencia, Spain.
authors.forename: Frederic
authors.initials: F
authors.lastname: Aparicio
authors.forename: Vicente
authors.initials: V
authors.lastname: Pallás
authors.forename: Jesús
authors.initials: J
authors.lastname: Sánchez-Navarro
mesh_terms: D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D053830:Plant Viral Movement Proteins / Q000235:genetics / Q000378:metabolism*; D010942:Plant Viruses / Q000502:physiology*; D027861:Prunus / Q000821:virology*; D014026:Nicotiana / Q000821:virology
chemical_list: D036022:Capsid Proteins; D053830:Plant Viral Movement Proteins