title: [The influence of Ara-C on anti-CD3/anti-Pgp mediating T-lymphocytes activities against multi-drug resistant leukemia cells.].
abstract: OBJECTIVE
  To investigate the role of Ara-C in regulating anti-CD3/anti-Pgp mediating T-lymphocytes activities against multi-drug resistant leukemia cells.
  
  
  METHODS
  The diabody of anti-CD3/anti-Pgp was purified by E-tag affinity chromatography. K562 and K562/A02 cells were treated with Ara-C. The expressions of B7-1 and B7-2 on K562 and K562/AO2 cells were detected by FACS. The cytotoxicity of T-lymphocytes combined with anti-CD3/anU-Pgp plus Ara-C was analyzed by CytoTox 96 nonradioactive method.
  
  
  RESULTS
  The expressions of B7-1 and B7-2 on K562 and K562/A02 cells treated by Ara-C was significantly higher than those untreated. The effect/target ratio was from 0.39:1 to 25:1, and the killing rate of activated T cells to anti-drug-resistant leukemia cells was from (16.44 +/- 1.20)% to (60.49 +/- 2.90)%. The killing rates were increased gradually, with both the effect/target ratio and the antibody concentration increasing (P < 0.05).
  
  
  CONCLUSIONS
  Ara-C may be an important adjuvant for improving anti-CD3/anti-Pgp mediating T-lymphocytes activities against multi-drug resistant leukemia cells.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Fan
authors.forename: Ying-Dai
authors.initials: YD
authors.lastname: Gao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Qing
authors.initials: Q
authors.lastname: Ji
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Shao
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Wang
authors.forename: Yuan-Fu
authors.initials: YF
authors.lastname: Xu
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Xiong
authors.forename: Chun-Zheng
authors.initials: CZ
authors.lastname: Yang
mesh_terms: D003561:Cytarabine; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D003561:Cytarabine