[Leukocyte stimulation by DNA fragments shored up protamine in cyclophosphamide-induced leukopoiesis in mice].
Our study showed that protamine (80% w/w to DNA) effectively protected its molecules from degradation by native nucleases of the mammalian blood serum. Exogenous DNA bound to protamine effectively stimulated restoration of cyclophosphamide-induced leukopoiesis in mice. It is suggested that the phenomenon was due to repair processes taking place in hemopoietic stem cells damaged by a cross-linking cytostatic drug such as cyclophosphamide. DNA dosage may be reduced and the original DNA fragment size maintained by DNA binding to protamine. As a result, it might involve longer DNA fragments into repair processes of homologous recombination and eventually increase the cell's chances of getting rid of extensive damage.
Dolgova
E V
EV
Rogachev
V A
VA
Nikolin
V P
VP
Popova
N A
NA
Likhacheva
A S
AS
Aliamkina
E A
EA
Sebeleva
T E
TE
Chernykh
E R
ER
Gel'fgat
E L
EL
Bogachev
S S
SS
Shudrov
M A
MA
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D019891:Leukopoiesis / Q000187:drug effects* / Q000235:genetics; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011479:Protamines / Q000378:metabolism*
D007166:Immunosuppressive Agents; D011479:Protamines; D003520:Cyclophosphamide