title: Melatonin protects against isoproterenol-induced myocardial injury in the rat: antioxidative mechanisms.
abstract: The present study was undertaken to explore the protective effect of melatonin against isoproterenol bitartrate (ISO)-induced myocardial injury in rat. Treatment of rats with ISO increased the level of lipid peroxidation products and decreased the reduced glutathione levels in cardiac tissue indicating that this synthetic catecholamine induces oxidative damage following oxidative stress. Pretreatment of ISO-injected rats with melatonin at a dose of 10 mg/kg body weight, i.p. prevented these changes. Additionally, melatonin also restored the activities and the levels of antioxidant enzymes which were found to be altered by ISO treatment. Treatment of rats with ISO resulted into an increased generation of hydroxyl radicals with melatonin pretreatment significantly reducing their production. Finally, treatment of rats with ISO caused a lowering of systolic pressure with reduced cardiac output and diastolic dysfunction whereas melatonin pretreatment significantly restored many of these parameters to normal. The findings document melatonin's ability to provide cardio protection at a low pharmacological dose. Melatonin has virtually no toxicity which raises the possibility of this indole being a therapeutic treatment for ischemic heart disease.
authors.affiliation: Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, University College of Science and Technology, Kolkata, India.
authors.forename: Debasri
authors.initials: D
authors.lastname: Mukherjee
authors.affiliation: Molecular Endocrinology Laboratory, Indian Institute of Chemical Biology, Kolkata, India.
authors.forename: Sreerupa Ghose
authors.initials: SG
authors.lastname: Roy
authors.affiliation: Molecular Endocrinology Laboratory, Indian Institute of Chemical Biology, Kolkata, India.
authors.forename: Arun
authors.initials: A
authors.lastname: Bandyopadhyay
authors.affiliation: Department of Physiology, Vidyasagar College, Kolkata, India.
authors.forename: Aindrila
authors.initials: A
authors.lastname: Chattopadhyay
authors.affiliation: Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, University College of Science and Technology, Kolkata, India.
authors.forename: Anjali
authors.initials: A
authors.lastname: Basu
authors.affiliation: Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, University College of Science and Technology, Kolkata, India.
authors.forename: Elina
authors.initials: E
authors.lastname: Mitra
authors.affiliation: Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, University College of Science and Technology, Kolkata, India.
authors.forename: Arnab Kr
authors.initials: AK
authors.lastname: Ghosh
authors.affiliation: Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, TX, USA.
authors.forename: Russel J
authors.initials: RJ
authors.lastname: Reiter
authors.affiliation: Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, University College of Science and Technology, Kolkata, India.
authors.forename: Debasish
authors.initials: D
authors.lastname: Bandyopadhyay
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000378:metabolism; D015153:Blotting, Western; D002374:Catalase; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006439:Hemodynamics / Q000187:drug effects; D007545:Isoproterenol / Q000037:antagonists & inhibitors* / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D009203:Myocardial Infarction / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D005978:Glutathione; D008550:Melatonin; D007545:Isoproterenol