title: [Regulation of synthesis function of porcine ovarian granulose cells by mitogen-activated protein kinase signaling pathway.].
abstract: OBJECTIVE
  To investigate the role of mitogen-activated protein kinase (MAPK) signaling pathway regulating synthesis function of steroid hormone of porcine ovarian granulose cells.
  
  
  METHODS
  Porcine ovarian granulose cells were cultured in medium added with dimethyl sulfoxide (DMSO) at concentration of 50 micromol/L or with MAPK inhibitor PD98059 (PD) for 48 hours. Then, granulose cells in DMSO medium were added with activator of adenylate cyclase (20 micromol/L) or blank agent for next 48 hours incubation, which were defined as observation group 1 and control group 1, similarly, granulose cells in PD medium were also added with activator of adenylate cyclase (20 micromol/L) or blank agent for next 48 hours, which were defined as observation group 2 and control group 2. The level of estradiol (E(2)), progesterone (P) and testosterone (T) were detected by chemoluminescence. The mRNA expression of 17alpha-hydroxylase/17,20-lyase (CYP17) and aromatase P450 (P450 arom) were assessed by RT-PCR.
  
  
  RESULTS
  (1) Hormone expression: the level of T, P and E(2) were (29.5 +/- 2.5) nmol/L, (80 +/- 5) nmol/L, (49 +/- 4) pmol/L in control group 1 and (42.3 +/- 3.4) nmol/L, (170 +/- 15) nmol/L, (75 +/- 6) pmol/L in control group 2, which showed significant difference (P < 0.05). In the mean time, the level of T, P and E(2) were (106.2 +/- 7.6) nmol/L, (210 +/- 16) nmol/L, (130 +/- 11) pmol/L in observation group 2 and (47.2 +/- 3.5) nmol/L, (130 +/- 6) nmol/L, (81 +/- 6) pmol/L in observation group 1, which also reach statistical difference (P < 0.05). (2) The expression of enzyme: the expression of CYP17 mRNA was increased by 50% and P450 arom mRNA was decreased by 20% between control group 1 and 2. However, the mRNA expression of P450 arom and CYP17 were upregulated remarkably, especially, the expression of CYP17 mRNA were increased by 125%.
  
  
  CONCLUSIONS
  MAPK signaling pathway plays an inhibitory role in regulating synthesis of steroid hormone of ovarian granulose cell.
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Qiu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Cong
authors.forename: Xiao-Ke
authors.initials: XK
authors.lastname: Wu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006107:Granulosa Cells / Q000378:metabolism; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D011374:Progesterone; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics; D013552:Swine
chemical_list: D012333:RNA, Messenger; D011374:Progesterone; D013254:Steroid 17-alpha-Hydroxylase; D020928:Mitogen-Activated Protein Kinases