Preparation of lipid membrane surfaces for molecular interaction studies by surface plasmon resonance biosensors.
Surface plasmon resonance has become one of the most important techniques for studying bimolecular interactions. Most of the researchers are using it to study protein-protein interactions, but in recent years membrane model systems have also become available and this makes it possible to study protein-membrane interactions as well. In this review chapter we describe possible ways to prepare lipid membrane surfaces on various sensor chips and some of the experimental considerations one has to take into account when performing such experiments.
Besenicar
Mojca Podlesnik
MP
Department of Biology, Biotechnical Faculty, University of Ljubljana, Institute "Jozef Stefan", Ljubljana, Slovenia.
Anderluh
Gregor
G
D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism*; D017743:Equipment Reuse; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D050356:Lipid Metabolism; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D011485:Protein Binding; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
D008051:Lipid Bilayers; D008081:Liposomes; D008565:Membrane Proteins