title: Note: Application of laser produced plasma K alpha x-ray probe in radiation biology.
abstract: A dedicated radiation biology x-ray generation and exposure system has been developed. 8.0 keV in energy x-ray pulses generated with a femtosecond-laser pulse was used to irradiate sample cells through a custom-made culture dish with a silicon nitride membrane. The x-ray irradiation resulted in DNA double-strand breaks in the nucleus of a culture cell that were similar to those obtained with a conventional x-ray source, thus demonstrating the feasibility of radiobiological studies utilizing a single burst of x-rays focused on single cell specimens.
authors.affiliation: Quantum Beam Science Directorate, Japan Atomic Energy Agency, Advanced Photon Research Center, 8-1-7 Umemidai, Kizugawa, Kyoto 619-0215, Japan. nishikino.masaharu@jaea.go.jp
authors.forename: Masaharu
authors.initials: M
authors.lastname: Nishikino
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Sato
authors.forename: Noboru
authors.initials: N
authors.lastname: Hasegawa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ishino
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ohshima
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Okano
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kawachi
authors.forename: Hodaka
authors.initials: H
authors.lastname: Numasaki
authors.forename: Tetruki
authors.initials: T
authors.lastname: Teshima
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nishimura
mesh_terms: D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D003300:Copper; D006657:Histones / Q000378:metabolism; D006801:Humans; D007834:Lasers; D011829:Radiation Dosage; D011853:Radiobiology / Q000295:instrumentation*; D014965:X-Rays
chemical_list: C468783:H2AX protein, human; D006657:Histones; D003300:Copper