Crystallization and preliminary crystallographic analysis of manganese(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from Bacillus sp. JF8.
A thermostable manganese(II)-dependent 2,3-dihydroxybiphenyl-1,2-dioxygenase derived from Bacillus sp. JF8 was crystallized. The initial screening for crystallization was performed by the sitting-drop vapour-diffusion method using a crystallization robot, resulting in the growth of two crystal forms. The first crystal belonged to space group P1, with unit-cell parameters a = 62.7, b = 71.4, c = 93.6 A, alpha = 71.2, beta = 81.0, gamma = 64.0 degrees, and diffracted to 1.3 A resolution. The second crystal belonged to space group I222, with unit-cell parameters a = 74.2, b = 90.8, c = 104.3 A, and diffracted to 1.3 A resolution. Molecular-replacement trials using homoprotocatechuate 2,3-dioxygenase from Arthrobacter globiformis (28% amino-acid sequence identity) as a search model provided a satisfactory solution for both crystal forms.
Senda
Miki
M
Structure Guided Drug Development Project, Research and Development Department, Japan Biological Informatics Consortium, 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.
Hatta
Takashi
T
Kimbara
Kazuhide
K
Senda
Toshiya
T
D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D049308:Dioxygenases / Q000737:chemistry* / Q000378:metabolism; D008345:Manganese / Q000378:metabolism*; D008958:Models, Molecular; D017434:Protein Structure, Tertiary
D008345:Manganese; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase