title: Potentiation of bacterial killing activity of zinc chloride by pyrrolidine dithiocarbamate.
abstract: Zinc has antimicrobial activity and zinc salts including zinc chloride (ZnCl(2)) have been used for the control of oral malodor. In this study, we hypothesized that pyrrolidine dithiocarbamate (PDTC), a zinc ionophore, may enhance antimicrobial efficacy of ZnCl(2). The bactericidal effectiveness of ZnCl(2) alone (0.5-8 mM) or in combination with PDTC (1 or 10 microM) was evaluated by in vitro short (1 h) time-killing assays against Fusobacterium nucleatum and Porphyromonas gingivalis. Only a slight viability decrease was observed with ZnCl(2) or PDTC alone after 1-h incubation. By contrast, combination of ZnCl(2) and PDTC could achieve a more than 100-fold viability reduction compared with ZnCl(2) or PDTC alone in F. nucleatum and P. gingivalis. Therefore, PDTC greatly enhanced the bactericidal activity of ZnCl(2) against the oral malodor-producing bacteria. These results suggest that use of PDTC may be useful for enhancing bactericidal activity of antimalodor regimens of zinc salts.
authors.affiliation: Brain Korea 21 Program, Chonnam National University Dental School, Gwangju, Republic of Korea.
authors.forename: Eun-Kyoung
authors.initials: EK
authors.lastname: Choi
authors.forename: Hye-Hyang
authors.initials: HH
authors.lastname: Lee
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Kang
authors.forename: Byung-Gook
authors.initials: BG
authors.lastname: Kim
authors.forename: Hoi-Soon
authors.initials: HS
authors.lastname: Lim
authors.forename: Seon-Mi
authors.initials: SM
authors.lastname: Kim
authors.forename: In-Chol
authors.initials: IC
authors.lastname: Kang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002712:Chlorides / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004357:Drug Synergism; D016967:Fusobacterium nucleatum / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000254:growth & development; D011759:Pyrrolidines / Q000494:pharmacology*; D013859:Thiocarbamates / Q000494:pharmacology*; D017967:Zinc Compounds / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D002712:Chlorides; D011759:Pyrrolidines; D013859:Thiocarbamates; D017967:Zinc Compounds; C020972:pyrrolidine dithiocarbamic acid; C016837:zinc chloride