Capture of intact liposomes on biacore sensor chips for protein-membrane interaction studies.
Qualitative and quantitative aspects of protein interactions with membranes may be studied by optical sensors. Biacore offers two dedicated chips for working with lipids and membranes: the L1 and HPA sensor chips. The L1 chip is the most frequently used in protein-membrane interaction studies and it allows the capture of intact liposomes. This chapter describes the protocol for immobilization of liposomes on L1 sensor chips and discusses some of the experimental considerations. An alternative approach that utilizes a streptavidin-coated sensor chip (SA sensor chip) is described for cases when it is not possible to use an L1 chip.
Hodnik
Vesna
V
Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Anderluh
Gregor
G
D000818:Animals; D001710:Biotin / Q000378:metabolism; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D019809:Streptavidin / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
D008081:Liposomes; D011506:Proteins; D001710:Biotin; D019809:Streptavidin