A reverse transcriptase stop assay revealed diverse quadruplex formations in UTRs in mRNA.
Here, we developed a reverse transcriptase based method (RTase stop assay) to characterize quadruplex formations in guanine-rich RNAs with high sensitivity and specificity. By using the RTase stop assay, we also revealed a plausible structural polymorphism in biologically important RNAs. The RTase stop assay would provide helpful insight into RNA quadruplex structures and functions, together with other analytical methods, including various footprinting techniques.
Hagihara
Masaki
M
Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research, Osaka University, Ibaraki 567-0047, Japan.
Yoneda
Keisuke
K
Yabuuchi
Hiroaki
H
Okuno
Yasushi
Y
Nakatani
Kazuhiko
K
D054856:G-Quadruplexes; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D020506:Untranslated Regions
D012333:RNA, Messenger; D020506:Untranslated Regions; D012194:RNA-Directed DNA Polymerase