Isolation of mesenchymal stem cells with neurogenic potential from the mesoderm of the amniotic membrane.
The amniotic membrane has been clinically applied as a therapeutic material in wound covering and corneal surface reconstruction. Recently, mesenchymal stem cells (MSCs) have been isolated from the placenta, specifically from the amniotic membrane. However, the localization of MSCs in the amniotic membrane has not been determined. In this study, term placenta was collected, and we performed immunohistochemical staining techniques to identify and localize MSCs in the mesoderm of the amniotic membrane in situ with MSC antibodies, including CD90 and CD105. We further directly cultured and characterized MSCs from the amniotic membrane mesoderm (AMSCs). The AMSCs were easily isolated and represented a homogenous fibroblastic morphology at early passages. In addition to MSC surface markers, AMSCs expressed Sox2, Oct-4 and Nanog. AMSCs could be induced into osteocytes, adipocytes and chondrocytes in vitro and show immunosuppressive effects on T-cell proliferation. Under appropriate conditions, AMSCs could differentiate into neuronal-like cells, which were identified by neuronal-specific markers and their ability to secrete dopamine. This study reveals that AMSCs provide a promising source for stem cell studies and also extend the clinical potential of the amniotic membrane in the field of regenerative medicine.
Chang
Yu-Jen
YJ
Bioresource Collection and Research Center, Food Industry Research and Development Institute, National Chiao Tung University, Hsinchu, Taiwan, ROC.
Hwang
Shiaw-Min
SM
Tseng
Ching-Ping
CP
Cheng
Fu-Chou
FC
Huang
Shih-Hung
SH
Hsu
Lee-Feng
LF
Hsu
Li-Wen
LW
Tsai
Ming-Song
MS
D000328:Adult; D000650:Amnion / Q000166:cytology*; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods*; D019902:Chondrocytes / Q000166:cytology; D004298:Dopamine / Q000378:metabolism; D000071063:Endoglin; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology*; D009474:Neurons / Q000502:physiology*; D010011:Osteocytes / Q000166:cytology; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000378:metabolism; D018800:Thy-1 Antigens / Q000378:metabolism
D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface; D018800:Thy-1 Antigens; D004298:Dopamine