Rapid quantification of itraconazole-resistant Aspergillus fumigatus in air.
Solid-phase cytometry (SPC) was used to determine the total number and the number of itraconazole-resistant Aspergillus fumigatus cells in 60 air samples. Of the 570 A. fumigatus cells that were recovered, 10 (1.8%) were resistant. SPC proved more specific and rapid than culture and allowed high-troughput susceptibility testing.
Vanhee
Lies M E
LM
Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000, Ghent, Belgium.
Perman
Dana
D
Nelis
Hans J
HJ
Coenye
Tom
T
D000391:Air Microbiology; D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000302:isolation & purification; D015169:Colony Count, Microbial / Q000379:methods; D025141:Drug Resistance, Fungal; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology*; D009172:Mycology / Q000379:methods*
D000935:Antifungal Agents; D017964:Itraconazole