[Study on the optimal extraction process of chaihushugan powder].
OBJECTIVE
To study the optimal extraction process of chaihushugan powder by orthogonal design.


METHODS
RP-HPLC method was developed for the determination of saikosaponin a, ferulic acid, hesperidin and paeoniflorin in chaihushugan powder. The contents of the components and the extraction yield were selected as assessment indices. Four factors were study by L9 (3(4)), including the alcohol concentration, amount of alcohol, duration of extraction and times of extraction.


RESULTS
The optimal extracting condition was 80% alcohol consumed as 10 times of crude herb amount, and extracting two times for 90 min each time.


CONCLUSIONS
This study supplies theoretical base for the development of chaihushugan powder formulation.
Wang
Chun-yan
CY
Department of Pharmanacy, Hebei North College, Zhangjiakou 075000, China. wangxiaoya051001@126.com
Zhang
Wan-ming
WM
Zhang
Dan-shen
DS
An
Fang
F
Tian
Jia-ming
JM
D001565:Benzoates / Q000032:analysis / Q000302:isolation & purification; D001952:Bridged-Ring Compounds / Q000032:analysis / Q000302:isolation & purification; D029972:Bupleurum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis / Q000302:isolation & purification; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification; D006569:Hesperidin / Q000032:analysis / Q000302:isolation & purification; D006358:Hot Temperature; D039821:Monoterpenes; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D011208:Powders; D011786:Quality Control; D012503:Saponins / Q000032:analysis* / Q000302:isolation & purification; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D003373:Coumaric Acids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D000431:Ethanol; D005960:Glucosides; D006569:Hesperidin; D039821:Monoterpenes; D009828:Oleanolic Acid; D011208:Powders; D012503:Saponins; C015423:peoniflorin; D006027:Glycosides; C004999:ferulic acid; C025759:saikosaponin D