Borrelia Burgdorferi "sensu lato" in Brazil: Occurrence confirmed by immunohistochemistry and focus floating microscopy.
In the present study, we report the occurrence of Lyme's borreliosis in patients from the Brazilian Amazon Region. Borreliosis was investigated by immunohistochemistry and focus floating microscopy for Borrelia burgdorferi in skin biopsy samples from 22 patients with both clinical and histopathology evidences compatible with Erythema Migrans. Spirochetes were detected by specific immunohistochemistry and focus floating microscopy for B. burgdorferi in samples from five patients. Clinical cure of the cutaneous lesions was observed in all the patients after treatment with doxycycline regimen as proposed by the Center Disease Control guidelines. A limitation of our study was the fact that we were not able to isolate and culture these organisms. These are the first known Brazilian cases of borreliosis to have Focus Floating Microscopy confirmation.
Talhari
Sinésio
S
Fundação de Medicina Tropical do Amazonas, Manaus, Amazonas, Brazil.
de Souza Santos
Mônica Nunes
MN
Talhari
Carolina
C
de Lima Ferreira
Luiz Carlos
LC
Silva
Roberto Moreira
RM
Zelger
Bernhard
B
Massone
Cesare
C
Ribeiro-Rodrigues
Rodrigo
R
D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001706:Biopsy; D015748:Borrelia burgdorferi Group / Q000166:cytology / Q000302:isolation & purification*; D001938:Brazil / Q000453:epidemiology; D004318:Doxycycline / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008193:Lyme Disease / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D012867:Skin / Q000382:microbiology* / Q000473:pathology; D016896:Treatment Outcome
D000900:Anti-Bacterial Agents; D004318:Doxycycline