The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular factors.
ISWI is an evolutionarily conserved ATP-dependent chromatin remodeling factor playing central roles in DNA replication, RNA transcription, and chromosome organization. The variety of biological functions dependent on ISWI suggests that its activity could be highly regulated. Our group has previously isolated and characterized new cellular activities that positively regulate ISWI in Drosophila melanogaster. To identify factors that antagonize ISWI activity we developed a novel in vivo eye-based assay to screen for genetic suppressors of ISWI. Our screen revealed that ISWI interacts with an evolutionarily conserved network of cellular and nuclear factors that escaped previous genetic and biochemical analyses.
Arancio
Walter
W
Dipartimento di Scienze Biochimiche, Istituto Telethon Dulbecco, Universitá degli Studi di Palermo, Palermo, Italy.
Onorati
Maria C
MC
Burgio
Giosalba
G
Collesano
Marianna
M
Ingrassia
Antonia M R
AM
Genovese
Swonild I
SI
Fanto
Manolis
M
Corona
Davide F V
DF
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001681:Biological Assay; D002453:Cell Cycle; D017124:Conserved Sequence; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D019143:Evolution, Molecular; D005123:Eye / Q000473:pathology; D005470:Fluorometry; D016153:Genes, Suppressor; D005820:Genetic Testing; D009154:Mutation / Q000235:genetics; D009707:Nucleosomes / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
D029721:Drosophila Proteins; C086468:ISWI protein; D009707:Nucleosomes; D014157:Transcription Factors; D000251:Adenosine Triphosphatases