Genetic toxicology of dental composite resin extracts in somatic cells in vivo.
The aim of this study was to assess the potential genetic toxicity associated to nine aqueous extracts from dental composite resins (Charisma, Fill Magic, Fill Magic Flow, Durafill, TPH Spectrum, Concept, Natural Look, Filtek Z250 and Filtek P60) and one random extract. Homologous mitotic recombination, point and chromosomal mutation effects were determined in somatic proliferative cells of Drosophila melanogaster exposed to aqueous extracts of the clinically used composites. Reproducible increases in clone mutant spot frequencies induced by diluted extract of Fill Magic Flow were observed. These increments were exclusively associated to the induction of homologous recombination - a genetic phenomenon involved in the loss of heterozygosis. The other eight composite resins and the random extract had no statistically significant effect on total spot frequencies - suggesting that they are non-genotoxic in the somatic mutation and recombination test assay, which agrees with the applications they have in dentistry. These findings - supported by numerous studies showing a positive correlation between carcinogenicity in man and genotoxicity in the Drosophila wing spot test - point to the potential risks some composite resins pose to the health of patients and dentistry personnel.
Arossi
Guilherme Anziliero
GA
Laboratory of Genetic Toxicology, Post-graduation Program in Genetics and Applied Toxicology, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
Dihl
Rafael Rodrigues
RR
Lehmann
Mauricio
M
Reguly
Maria Luiza
ML
de Andrade
Heloísa Helena Rodrigues
HH
D000180:Acrylic Resins / Q000737:chemistry / Q000145:classification / Q000633:toxicity*; D000818:Animals; D003188:Composite Resins / Q000737:chemistry / Q000145:classification / Q000633:toxicity*; D004249:DNA Damage; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics; D008938:Mitosis / Q000187:drug effects; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000145:classification / Q000633:toxicity*; D017354:Point Mutation / Q000187:drug effects; D011140:Polyurethanes / Q000737:chemistry / Q000145:classification / Q000633:toxicity*; D011995:Recombination, Genetic / Q000187:drug effects; D014921:Wings, Animal / Q000187:drug effects
D000180:Acrylic Resins; C029012:Composite Dental Resin; D003188:Composite Resins; D009153:Mutagens; D011140:Polyurethanes