Acoustophoretic synchronization of mammalian cells in microchannels.
We report the first use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. The acoustophoretic cell synchronization (ACS) device utilizes volume-dependent acoustic radiation force within a microchannel to selectively purify target cells of desired phase from an asynchronous mixture based on cell cycle-dependent fluctuations in size. We show that ultrasonic separation allows for gentle, scalable, and label-free synchronization with high G(1) phase synchrony (approximately 84%) and throughput (3 x 10(6) cells/h per microchannel).
Thévoz
Patrick
P
Department of Mechanical Engineering, University of California, Santa Barbara, California 93106, USA.
Adams
Jonathan D
JD
Shea
Herbert
H
Bruus
Henrik
H
Soh
H Tom
HT
D000818:Animals; D002453:Cell Cycle; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D011419:Propidium / Q000737:chemistry; D014465:Ultrasonics
D011419:Propidium