[Research on technological process of two-pot countercurrent extraction of hydroxysafflor yellow A].
OBJECTIVE
To study the optimum technical conditions of extracting Hydroxysafflor yellow A (HSYA) from Carthmus tinctorius by multi-stage countercurrent extraction technology.


METHODS
The effects of extraction time of each stage, extraction temperature, ethanol concentration and solid-liquid ratio (g x mL(-1)) on extraction yield of HSYA were studied by orthogonal test design and the comparison of other extraction methods were presented.


RESULTS
Extraction time and solid-liquid ratio had significant influence on the extraction yield, and the optimum parameters were as follows: Extraction time of each stage was 120 min, solid-liquid ratio was 1 : 10 (g x mL(-1)), ethanol concentration was 30%, and extracted at room temperature. Under the optimum conditions, the extraction yield of HSYA was 1.56% and the purity of the extract was 6.06%. Compared with the traditional extraction method and the ultrasonic extraction method of the pharmacopoeia, the extraction yield was increased by 6.12% and 9.09%, the purity of extract was increased by 42.9% and 27.0%, respectively.


CONCLUSIONS
The multi-stage countercurrent extraction technology has many advantages such as simple operation, less solvent consumption, higher extraction yield and purity of extract and it has wide industrial application prospect.
Li
Yerui
Y
College of Pharmaceutical Science Zhejiang University, Hangzhou 310058, China. xiaozhanleery@163.com
Chen
Yong
Y
Wang
Longhu
L
Chen
Xueying
X
Liu
Xuesong
X
Sun
Changhai
C
Yan
Wenjie
W
D031178:Carthamus / Q000737:chemistry*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D003377:Countercurrent Distribution / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D011809:Quinones / Q000032:analysis / Q000302:isolation & purification*
D004365:Drugs, Chinese Herbal; D011809:Quinones; C085278:hydroxysafflor yellow A; D002599:Chalcone