Modification of polyglutamic acid with silanol groups and calcium salts to induce calcification in a simulated body fluid.
The formation of hydroxyapatite is important for artificial materials to show high biological affinities for bone tissue. The present study focused on the synthesis of hydrogels capable of showing apatite formation, through modification of polyglutamic acid (PGA) with 3-aminopropyltriethoxysilane (APTES), followed by treatment with calcium chloride solution. A transparent bulk hydrogel was obtained at a molar ratio of PGA/APTES of 0.5. Prior soaking of the PGA hydrogel in calcium chloride solution accelerated the formation of bone-like apatite in a simulated body fluid. The modified PGA hydrogel is a candidate material for a biodegradable scaffold for bone regeneration.
Koh
Mi-Young
MY
Graduate School of Engineering, Nagoya University, Furo-cho Chikusa-ku, Nagoya 464-8603, Japan. koh.miyoung@h.mbox.nagoya-u.ac.jp
Ohtsuki
Chikara
C
Miyazaki
Toshiki
T
D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry*; D002113:Calcification, Physiologic; D002114:Calcinosis / Q000139:chemically induced; D002122:Calcium Chloride / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry; D008422:Materials Testing / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008954:Models, Biological; D011099:Polyglutamic Acid / Q000032:analysis / Q000737:chemistry*; D011437:Propylamines; D012821:Silanes / Q000032:analysis / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties; D014961:X-Ray Diffraction / Q000379:methods
D001672:Biocompatible Materials; D020100:Hydrogels; D011437:Propylamines; D012821:Silanes; D012996:Solutions; C082343:silanol; D011099:Polyglutamic Acid; D017886:Durapatite; C477625:amino-propyl-triethoxysilane; D002122:Calcium Chloride