Role of decay-accelerating factor in regulating survival of human cervical cancer cells.
BACKGROUND
Decay-accelerating factor (DAF) is one of the key molecules involved in cell protection against autologous complement, which restricts the action of complement at critical stages of the cascade reaction. The effect of DAF on the survival of human cervical cancer cell (ME180) has not been demonstrated.


METHODS
In this study we applied, for the first time, small interference RNA (siRNA) to knock down the expression of the DAF with the aim of exploiting complement more effectively for tumor cell damage. Meanwhile, we investigated the effects of DAF on the viability and migration, moreover the proliferation of ME180 cell.


RESULTS
The results showed that the expression of DAF was significantly increased in human cervical cancer tissues. SiRNA inhibition of DAF expression enhanced complement-dependent cytolysis up to 32% in ME180 cells, which contributed to the control of C3 activation and increased the cells viability, migration and augment the number of ME180 cells.


CONCLUSIONS
These data indicated that DAF siRNA described in this study may offer an additional alternative to improve the efficacy of antibody- and complement-based cancer immunotherapy.
Gao
Ling-Juan
LJ
Clinical Laboratory, Nanjing Maternity and Child Health Care Hospital, Tianfei Alley, Nanjing Mochou Road, Nanjing 210004, People's Republic of China.
Ding
Lan
L
Guo
Shu-Yu
SY
Cai
You-Qun
YQ
Su
Ya-Juan
YJ
Gong
Hui
H
Liu
Yun
Y
Chen
Chen
C
Gu
Ping-Qing
PQ
D018958:CD55 Antigens / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D050577:Complement C3-C5 Convertases / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006801:Humans; D034741:RNA, Small Interfering / Q000378:metabolism; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D018958:CD55 Antigens; D034741:RNA, Small Interfering; D050577:Complement C3-C5 Convertases