title: Semipurified fractions from the submerged-culture broth of Agaricus blazei Murill reduce blood glucose levels in streptozotocin-induced diabetic rats.
abstract: Hypoglycemic action of semipurified fractions from hot-water extracts of the submerged-culture broth of Agaricus blazei Murill was examined in streptozotocin (60 mg/kg, intraperitoneal)-induced diabetic male Sprague-Dawley rats, relative to the diabetes drug metformin. The hot-water extract, treated with ethanol to remove beta-glucans and glycoproteins, was freeze-dried, and fractionated into hexane, chloroform, ethyl acetate (EA), and butanol fractions. The EA fraction (EAF; 200 mg/kg body weight) reduced (p < 0.05) the blood glucose level in the oral glucose tolerance test, relative to the other fractions and control. In a 14 day-treatment study, diabetic rats treated with the EAF displayed a suppressed blood glucose level and elevated plasma insulin and glucose transport-4 proteins; the reactions occurred in a dose-dependent manner (200 and 400 mg/kg body weight) compared to those in control animals. The EAF reduced the levels of triglyceride and cholesterol in plasma, the activity of glutamate-oxaloacetate transaminase and glutamate-pyruvate transaminase in blood, and the content of thiobarbituric acid reactive substance in the liver and kidney. The hypoglycemic efficacy of the EAF (400 mg/kg body weight) was similar to that of metformin (500 mg/kg body weight). The EAF contained substantial amounts of isoflavonoids including genistein, genistin, daidzein, and daidzin, which could have contributed to the fraction's hypoglycemic action. These results indicate that the hot-water extract of the submerged-culture broth of Agaricus blazei contains an EAF having potent hypoglycemic action, which could be useful in the treatment of diabetes mellitus.
authors.affiliation: Division of Applied Life Sciences (BK21 program), Graduate School and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Tae W
authors.initials: TW
authors.lastname: Oh
authors.forename: Yun A
authors.initials: YA
authors.lastname: Kim
authors.forename: Wook J
authors.initials: WJ
authors.lastname: Jang
authors.forename: Jae I
authors.initials: JI
authors.lastname: Byeon
authors.forename: Chung H
authors.initials: CH
authors.lastname: Ryu
authors.forename: Jeong O
authors.initials: JO
authors.lastname: Kim
authors.forename: Yeong L
authors.initials: YL
authors.lastname: Ha
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D001685:Biological Factors / Q000008:administration & dosage* / Q000032:analysis / Q000302:isolation & purification*; D001786:Blood Glucose / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry*; D046508:Culture Techniques; D003920:Diabetes Mellitus / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D004195:Disease Models, Animal; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000032:analysis / Q000302:isolation & purification*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000009:adverse effects
chemical_list: D001685:Biological Factors; D001786:Blood Glucose; D003470:Culture Media; D007004:Hypoglycemic Agents; D013311:Streptozocin