[Complete sequence of the M segment of Amur virus in rodent from China].
OBJECTIVE
To study the complete sequence of M segment of Amur virus in rodents and to explore their molecular characteristics.


METHODS
Complete M segment of Amur virus in rodent from China was amplified by RT-PCR. The purified PCR product was cloned into pGEM-T Easy vector and then sequenced. Phylogenetic analysis on multiple nucleotide sequences was performed with the Tree PUZZLE and DNAStar software.


RESULTS
The full-length of its M gene comprised of 3615 nucleotides with one open reading frame (ORF) including 3408 nucleotides and encoding a protein which comprised 1135 amino acids. The ORF was located at bases 41 to 3448. The phylogenetic analysis of JilinAP06 with other hantaviruses revealed that the complete sequence of M segment of JilinAP06 strain was closely related to those Amur viruses such as B78 strain, Liu strain and H5 strain were all from the patients. The complete sequence of M segment of JilinAP06 had only 79.5% identities with the nucleotide sequence of HTNV strain 76 - 118.


CONCLUSIONS
The complete sequence on M segment of Amur virus in rodent was first time identified in this country.
Guo
Wei-dong
WD
Inner Mongolia Autonomous Region Center for Disease Control and Prevention, Huhehaote 010031, China.
Zhang
Wen-yi
WY
Jiang
Jia-fu
JF
Chi
Hai-yi
HY
Wang
Wen-rui
WR
Wang
Zhong-yuan
ZY
Jin
Ying
Y
Yang
Hong
H
Fang
Li-qun
LQ
Cao
Wu-chun
WC
D000818:Animals; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D006476:Orthohantavirus / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012377:Rodentia / Q000821:virology*