[Fabrications of a poly (methyl methacrylate) (PMMA) microfluidic chip-based DNA analysis device].
A DNA analysis device based on poly(methyl methacrylate) (PMMA) microfluidic chips was developed. A PMMA chip with cross microchannels was fabricated by a simple hot embossing. Microchannels were modified with a static adsorptive coating method using 2% hydroxyethyl cellulose. A high-voltage power unit, variable in the range 0-1 800 V, was used for on-chip DNA sample injection and gel electrophoretic separation. High speed, high resolution DNA analysis was obtained with the home-built PMMA chip in a sieving matrix containing 2% hydroxyethyl cellulose with a blue intercalating dye, TO-PRO-3 (TP3), by using diode laser induced fluorescence detection based on optical fibers with a 670 nm long-pass filter. The DNA analysis device was applied for the separation of phiX-174/HaeIII DNA digest sample with 11 fragments ranging from 72 to 1 353 bp. A separation efficiency of 1.14 x 10(6) plates/m was obtained for the 603 bp fragments, while the R of 271/281 bp fragments was 1.2. The device was characterized by simple design, low cost for fabrication and operation, reusable PMMA chips, and good reproducibility. A portable microfluidic device for DNA analysis can be developed for clinical diagnosis and disease screening.
Du
Xiao-Guang
XG
Research Center for Analytical Sciences, College of Science, Northeastern University, Shenyang 110004, China. xgyydu@yahoo.com.cn
D002482:Cellulose / Q000031:analogs & derivatives; D004396:Coloring Agents; D004247:DNA / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques; D019904:Polymethyl Methacrylate
D004396:Coloring Agents; D002482:Cellulose; C002283:hydroxyethylcellulose; D004247:DNA; D019904:Polymethyl Methacrylate