Site-specific pKa determination of the carboxylate-binding subunit in artificial peptide receptors.
Site-specific pK(a) determination of the carboxylate-binding subunit in artificial peptide receptors is achieved by pH-dependent UV resonance Raman scattering with subsequent matrix factorization. This quantitative methodology allows to determine accurately the local acidity of a binding site based on the complete vibrational spectrum and, most importantly, without the need of selecting any particular peak.
Niebling
Stephan
S
University of Osnabrück, Department of Physics, Barbarastr. 7, 49069 Osnabrück, Germany.
Srivastava
Sunil K
SK
Herrmann
Christoph
C
Wich
Peter R
PR
Schmuck
Carsten
C
Schlücker
Sebastian
S
D002264:Carboxylic Acids / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D018000:Receptors, Peptide / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D002264:Carboxylic Acids; D018000:Receptors, Peptide