title: Isolation and characterization of goat retinal microvascular endothelial cells.
abstract: In this report, we demonstrate a method for the isolation of pure homogeneous endothelial cell population from goat's eye without using multi-step procedure and sophisticated instrument facilities. Microvascular endothelial cell from goat's retina were isolated using enzymatic method and cultured in Iscove's Modified Dulbecco's Medium supplemented with 10% fetal bovine serum. Five to 7 d after plating, a monolayer of endothelial cells was formed. These cells were identified as endothelial cells by morphology and confirmed by positive immunocytochemistry for vWF, CD31, VE-cadherin, CD146, VCAM-1, and ICAM-1, a specific marker for endothelial cells. We have compared both the mechanical and non-mechanical enzymatic methods in isolating pure endothelial cells. Cells plated on 4% gelatin-coated dishes resulted in tubular morphology, a characteristic of endothelial cells. This method is simpler and cost-effective when compared with other previously reported methods. These endothelial cells will be more helpful to identify the role of various factors in angiogenic-related disease such as diabetic retinopathy.
authors.affiliation: Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, Krishnankoil, 626 190, Tamilnadu, India.
authors.forename: Ravinarayanan
authors.initials: R
authors.lastname: Haribalaganesh
authors.forename: Elayappan
authors.initials: E
authors.lastname: Banumathi
authors.forename: Sardarpasha
authors.initials: S
authors.lastname: Sheikpranbabu
authors.forename: Venkataraman
authors.initials: V
authors.lastname: Deepak
authors.forename: Namagiri
authors.initials: N
authors.lastname: Sirishkumar
authors.forename: Sangiliyandi
authors.initials: S
authors.lastname: Gurunathan
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology*; D007150:Immunohistochemistry; D012171:Retinal Vessels / Q000166:cytology* / Q000378:metabolism