title: [Study of methylation of promoter of EYA1 gene in microtia].
abstract: OBJECTIVE
  To explore the methylation of CpG islands in promoter of eye absent gene 1 (EYA1) in microtia.
  
  
  METHODS
  The methylation of CpG islands in EYA1 gene in 64 microtias and 36 healthy controls were measured using the technique of matrix-assisted laser desorption/ionization-time of flight.
  
  
  RESULTS
  The methylation of CpG_Unit3 and CpG_Unit5 of EYA1 gene in microtia were 0.09258 +/- 0.033846 and 0.0922 +/- 0.02379, respectively, which were significantly lower than those in control.
  
  
  CONCLUSIONS
  Hypomethylation in microtia may be related to the pathogenesis of the disease.
authors.affiliation: Plastic Surgery Hospital, Chinese Academy of Medical Science, Beijing 100144, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Bo
authors.initials: B
authors.lastname: Pan
authors.forename: Hai-yue
authors.initials: HY
authors.lastname: Jiang
authors.forename: Hong-xing
authors.initials: HX
authors.lastname: Zhuang
authors.forename: Yan-yong
authors.initials: YY
authors.lastname: Zhao
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Yang
authors.forename: Le-ren
authors.initials: LR
authors.lastname: He
authors.forename: Juan
authors.initials: J
authors.lastname: Han
authors.forename: Shu-jie
authors.initials: SJ
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D018899:CpG Islands; D019175:DNA Methylation; D004423:Ear / Q000002:abnormalities*; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; C104381:EYA1 protein, human; D017027:Protein Tyrosine Phosphatases