A novel cold-regulated gene from Camellia sinensis, CsCOR1, enhances salt- and dehydration-tolerance in tobacco.
In present research, the full-length cDNA and the genomic sequence of a novel cold-regulated gene, CsCOR1, were isolated from Camellia sinensis L. The deduced protein CsCOR1 contains a hydrophobic N-terminus as a signal peptide and a hydrophilic C-terminal domain that is rich in glycine, arginine and proline. Two internal repetitive tridecapeptide fragments (HSVTAGRGGYNRG) exist in the middle of the C-terminal domain and the two nucleotide sequences encoding them are identical. CsCOR1 was localized in the cell walls of transgenic-tobaccos via CsCOR1::GFP fusion approach. The expression of CsCOR1 in tea leaves was enhanced dramatically by both cold- and dehydration-stress. And overexpression of CsCOR1 in transgenic-tobaccos improved obviously the tolerance to salinity and dehydration.
Li
Xian-Wen
XW
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Feng
Zhi-Guo
ZG
Yang
Hui-Min
HM
Zhu
Xiao-Pei
XP
Liu
Jun
J
Yuan
Hong-Yu
HY
D000595:Amino Acid Sequence; D028241:Camellia sinensis / Q000235:genetics* / Q000502:physiology; D003080:Cold Temperature; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology; D017434:Protein Structure, Tertiary; D055049:Salt Tolerance / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics* / Q000502:physiology
D010940:Plant Proteins