[Screening and identification of a fungal strain functioning in hydroxylation of puerarin].
OBJECTIVE
Puerarin is a Chinese herbal medicine. We isolated a fungus from soil which could convert puerarin to 3'-hydroxypuerarin.


METHODS
Strains were cultivated on potato dextrose agar (PDA). The biotransformation product was extracted with an organic solvent and detected on high performance liquid chromatography(HPLC).


RESULTS
A total of 186 fungal strains were isolated from soil, and strain NT-01 was determined to transform puerarin into 3'-hydroxypuerarin. The product was identified as 3'-hydroxypuerarin by MS and 1H-NMR and 13C-NMR. The morphology characteristic of strain NT-01 was consistent with Gliocladium deliguescens. Phylogenic analysis on sequences showed that strain NT-01 was clustered together with G. deliguescens and G. viride with high boostrap supporting, so that strain NT-01 was identified as Gliocladium sp.


CONCLUSIONS
Gliocladium strain NT-01 can convert puerarin to 3'-hydroxypuerarin.
Yang
Liping
L
College of Life Science, Nantong University, Nantong 226007, China. ylp325@tom.com
Xu
Lei
L
Zhu
Xinyu
X
Yu
Lijuan
L
Tang
Susu
S
Cui
Zhichao
Z
Ye
Hui
H
D001711:Biotransformation; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006900:Hydroxylation; D007529:Isoflavones / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012988:Soil Microbiology
D007529:Isoflavones; C033607:puerarin