title: Expressions of heat shock and metallothionein genes in the heart of common carp (Cyprinus carpio): effects of temperature shock and heavy metal exposure.
abstract: Heat shock proteins (HSPs) and metallothioneins (MTs) play important roles in protection against environmental stressors. The present study analyzes and compares the regulation of heat shock ( hsp70, hsc70-1 and hsp90alpha ) and metallothionein (MT-1 and MT-2) genes in the heart of common carp, in response to elevated temperature, cold shock and exposure to several heavy metal ions (As 3+ , Cd 2+ and Cu 2+ ), in whole-animal experiments. Among these metals, arsenate proved to be the most potent inducer of the examined stress genes; the hsp90alpha and MT-1 mRNA levels were elevated 11- and 10-fold, respectively, after a 24-h exposure. In contrast, Cd 2+ at 10 mg/L had no impact on the expression of hsp90alpha , and the MT genes also proved to be rather insensitive to Cd 2+ treatment in the heart: only a 2-2.5-fold induction was observed in response to 10 mg/L Cd 2+ . Heat shock resulted in a transient induction of hsp70 (19-fold) and hsp90alpha (15-fold), while elevated temperature had no effect on the expression of the MTs. Direct cold shock induced hsp70 expression (14-fold), while the hsp90alpha (26-fold) and MT-2 (2-fold) expressions peaked after the recovery period following a direct cold shock. The five stress genes examined in this study exhibited a unique, tissue-specific basal expression pattern and a characteristic sensitivity to metal treatments and temperature shocks.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of Szeged, Szeged, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Said Ali
authors.forename: Agnes
authors.initials: A
authors.lastname: Ferencz
authors.forename: J
authors.initials: J
authors.lastname: Nemcsók
authors.forename: Edit
authors.initials: E
authors.lastname: Hermesz
mesh_terms: D000818:Animals; D001151:Arsenic / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology; D002347:Carps / Q000033:anatomy & histology / Q000378:metabolism; D003300:Copper / Q000494:pharmacology; D005334:Fever / Q000378:metabolism; D006321:Heart / Q000187:drug effects / Q000502:physiology; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D007035:Hypothermia / Q000378:metabolism; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism; D019216:Metals, Heavy / Q000494:pharmacology*; D013696:Temperature
chemical_list: D006360:Heat-Shock Proteins; D019216:Metals, Heavy; D002104:Cadmium; D003300:Copper; D008668:Metallothionein; D001151:Arsenic