Amelioration of streptozotocin-induced diabetes by Agrocybe chaxingu polysaccharide.
The aim of this study was to investigate the preventive effect of Agrocybe chaxingu polysaccharide on streptozocin (STZ)-induced pancreatic beta-cells destruction. Agrocybe chaxingu polysaccharide markedly reduced nitric oxide (NO) production and iNOS expression levels in RINm5F cells in a dose-dependent manner. In addition, Agrocybe chaxingu polysaccharide significantly inhibited iNOS expression and blood glucose levels in STZ-induced diabetic mice. Moreover, immunohistochemical analysis revealed that it enhanced pancreatic beta-cells resistance to destruction by STZ. These results suggest that Agrocybe chaxingu polysaccharide may have value as a therapeutic agent against diabetes mellitus.
Lee
Byung Ryong
BR
Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon, 200-702, Korea.
Lee
Yeom Pyo
YP
Kim
Dae Won
DW
Song
Ha Yong
HY
Yoo
Ki-Yeon
KY
Won
Moo Ho
MH
Kang
Tae-Cheon
TC
Lee
Kwang Jae
KJ
Kim
Kyung Hee
KH
Joo
Jin Ho
JH
Ham
Hun Ju
HJ
Hur
Jang Hyun
JH
Cho
Sung-Woo
SW
Han
Kyu Hyung
KH
Lee
Kil Soo
KS
Park
Jinseu
J
Eum
Won Sik
WS
Choi
Soo Young
SY
D055362:Agrocybe / Q000737:chemistry*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013311:Streptozocin
D001786:Blood Glucose; D020030:Nitric Oxide Donors; D011134:Polysaccharides; D009599:Nitroprusside; D009569:Nitric Oxide; D013311:Streptozocin; D052247:Nitric Oxide Synthase Type II