DNA-functionalized solid state nanopore for biosensing.
The possible use of nanopores for single DNA molecules biosensing has been demonstrated, but much remains to do in order to develop advanced engineered devices with enhanced stability, and controlled geometry and surface properties. Here we present morphological and electrical characterization of solid state silicon nitride nanopores fabricated by focused ion beam direct milling and chemically functionalized by probe oligonucleotides, with the final aim of developing a versatile tool for biosensing and gene expression profiling.
Mussi
V
V
Nanomed Labs, Physics Department, University of Genova, Advanced Biotechnology Center, Largo R. Benzi, Genova, Italy. mussi@fisica.unige.it
Fanzio
P
P
Repetto
L
L
Firpo
G
G
Scaruffi
P
P
Stigliani
S
S
Tonini
G P
GP
Valbusa
U
U
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D004553:Electric Conductivity; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity
D008567:Membranes, Artificial; D004247:DNA