Protective effects of Cinnamomum cassia Blume in the fibrogenesis of activated HSC-T6 cells and dimethylnitrosamine-induced acute liver injury in SD rats.
Cinnamomum cassia Blume (CC) is one of the world's oldest natural spices, and is commonly used in traditional oriental medicine. We investigated the protective effect of ethanol extract from Cinnamomum cassia Blume (CCE) on the activation of hepatic stellate cells (HSCs). In addition, we examined the effects of CC powder in Sprague-Dawley rats with acute liver injury induced by dimethylnitrosamine (DMN). In vitro, HSC-T6 cells exhibit an activated phenotype, as reflected in their fibroblast-like morphology. CCE significantly reduced the expression of alpha-smooth muscle actin (alpha-SMA), connective tissue growth factor (CTGF), transforming growth factor beta (TGF-beta1), and tissue inhibitor of metalloproteinase-1 (TIMP-1). In vivo, the results were significantly protected by CC powder in the serum total protein, albumin, total-bilirubin, direct-bilirubin, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and alkaline phosphatase (ALP). We suggest that CC inhibits fibrogenesis, followed by HSC-T6 cell activation and increased restoration of liver function, ultimately resulting in acute liver injury.
Lim
Chang-Shin
CS
College of Oriental Medicine and Institute of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
Kim
Eun-Young
EY
Lee
Hyun-Sam
HS
Soh
Yunjo
Y
Sohn
Youngjoo
Y
Kim
Sun Yeou
SY
Sohn
Nak-Won
NW
Jung
Hyuk-Sang
HS
Kim
Yoon-Bum
YB
D000199:Actins / Q000037:antagonists & inhibitors; D000818:Animals; D032904:Cinnamomum aromaticum / Q000737:chemistry*; D055513:Connective Tissue Growth Factor / Q000037:antagonists & inhibitors; D019610:Cytoprotection; D004128:Dimethylnitrosamine / Q000633:toxicity*; D055166:Hepatic Stellate Cells / Q000187:drug effects*; D008099:Liver / Q000187:drug effects; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000517:prevention & control*; D008111:Liver Function Tests; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000037:antagonists & inhibitors; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors
D000199:Actins; D055513:Connective Tissue Growth Factor; D004128:Dimethylnitrosamine; D010936:Plant Extracts; D019715:Tissue Inhibitor of Metalloproteinase-1; D053773:Transforming Growth Factor beta1; C498862:Acta2 protein, rat