Rapid detection of natriuretic peptides by a microfluidic LabChip analyzer with DNA aptamers: Application of natriuretic peptide detection.
Rapid detection of brain natriuretic peptide (BNP) concentration can be used for the diagnosis of acute heart failure and for the evaluation of the effectiveness of a clinical therapy. We used the systematic evolution of ligands by exponential enrichment method to develop DNA aptamers for BNP whose sequences were determined by cloning method and consensus sequence analysis. A total of eight conserved sequences was identified. By combining the fluorescent-labeled aptamers with fast protein lab-on-chip analysis, we could achieve quantification of BNP concentrations with high speed, sensitivity, and specificity.
Lin
Ming-Cheng
MC
Nawarak
Jiraporn
J
Chen
Tai-Yuan
TY
Tsai
Hsien-Yu
HY
Hsieh
Jung-Feng
JF
Sinchaikul
Supachok
S
Chen
Shui-Tein
ST