title: [Effect of freezing and pingyangmycin injection on the auricular posterior vein in rabbits].
abstract: OBJECTIVE
  To observe the change of vascular endothelial cells and structure of the auricular posterior vein caused by freezing or pingyangmycin injection alone and freezing in combination with pingyangmycin injection,to investigate the effect of these treatments on the vein.
  
  
  METHODS
  Eighteen rabbits were divided into 3 groups of 6 each, and another rabbit was used as a control. Pingyangmycin was injected into the auricular posterior vein in the first group, spray freezing within 20 seconds on the auricular posterior vein was performed in the second group,and freezing in combination with pingyangmycin injection was performed in the third group. Light microscope and transmission electron microscope were used to observe the change of auricular posterior vein in the rabbits.
  
  
  RESULTS
  Proliferation of endothelial cells,and thickening of vessel wall were induced by pingyangmycin. Thrombus formation, tissue oedema and inflammatory infiltration induced by spray freezing within 20 seconds were reversible. Thrombus formation, proliferation of endothelial cells and thickening of the vessel wall was induced by freezing in combination with pingyangmycin injection.
  
  
  CONCLUSIONS
  Freezing and pingyangmycin injection have the synergistic effect, resulting in the proliferation of endothelial cells, thrombus formation, thickening of vessel wall, and even occlusion of vessels.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Su
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Yifang
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D017679:Cryotherapy / Q000379:methods*; D004431:Ear, External / Q000098:blood supply*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D005260:Female; D005615:Freezing; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D014680:Veins / Q000002:abnormalities / Q000166:cytology / Q000187:drug effects*
chemical_list: D001761:Bleomycin; C025703:bleomycetin