title: The universal overexpression of a cancer testis antigen hiwi is associated with cancer angiogenesis.
abstract: The aim of this study was to determine whether the universal overexpression of hiwi is associated with cancer angiogenesis. The difference of mRNA expression in blood vessels derived from DLBCL and RLNH tissues was detected, and hiwi was overexpressed in tumor vessels of lymphoma. The hiwi expression in cell lines and tissues was measured by using RT-PCR and immuno-staining, as well as the Ang-2 and Tie-2 expressions in tissues. Hiwi was significantly expressed in cancer cell lines Hela, MCF-7, MDA-MB-231, MDA-MB-435s, A2780, 111 and 113. Compared with that of chronic cervicitis (CC), hyperplasia of mammary glands (HMG), ovarian benign lesions (OBL) and endometrium benign lesions (EBL), the expression of hiwi, Ang-2 and Tie-2 was increased significantly in uterine cervical cancer (UCC), breast carcinoma (BC), ovarian cancer (OC) and endometrial cancer (EC) (P<0.01). Additionally, the hiwi expression was positively correlated with Ang-2 in UCC, BC and OC, and Tie-2 in OC. These results suggest that hiwi plays an important role in the oncogenesis and is a potential target for cancer angiogenesis.
authors.affiliation: Cancer Biology Research Center, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Meng
authors.forename: Changhong
authors.initials: C
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Bai
authors.forename: Juncheng
authors.initials: J
authors.lastname: Wei
authors.forename: Yunping
authors.initials: Y
authors.lastname: Lu
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhou
authors.forename: Ding
authors.initials: D
authors.lastname: Ma
mesh_terms: D042702:Angiopoietin-2 / Q000096:biosynthesis / Q000235:genetics; D060565:Argonaute Proteins; D014408:Biomarkers, Tumor / Q000032:analysis; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D009369:Neoplasms / Q000098:blood supply* / Q000235:genetics / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D042787:Receptor, TIE-2 / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D042702:Angiopoietin-2; D060565:Argonaute Proteins; D014408:Biomarkers, Tumor; C421088:PIWIL1 protein, human; D011506:Proteins; D042787:Receptor, TIE-2