Lack of association of common allelic variants in the thyroglobulin gene with Hashimoto's thyroiditis in young subjects with type 1 diabetes.
OBJECTIVE
Four SNPs (E10SNP24, E10SNP158, E12SNP, E33SNP) in the Tg gene are suspected to be involved in the development of autoimmune thyroid diseases. The aim of the study was to determine whether these variants play a role in the development of Hashimoto's thyroiditis in young subjects with type 1 diabetes, in whom autoimmune thyroid diseases are significantly more common than in the general population.


METHODS
Seventy-six subjects with type 1 diabetes and Hashimoto's thyroiditis and 110 subjects with only type 1 diabetes were studied. Hashimoto's thyroiditis was determined according to the clinical, biochemical and ultrasonographic criteria. SNPs were determined by the TaqMan SNP method.


RESULTS
In young subjects with type 1 diabetes, no association between any of the tested SNPs or their combinations and Hashimoto's thyroiditis was found.


CONCLUSIONS
This is the first study to investigate the association of SNPs located inside the coding region of the Tg gene with the development of Hashimoto's thyroiditis in subjects with type 1 diabetes. The lack of an association is in concordance with a study where marker Tgms2, located inside intron 27, was not associated with joint susceptibility for autoimmune thyroid disease and type 1 diabetes.
Kotnik
Primoz
P
Department of Pediatric Endocrinology, UMC University Children's Hospital, Ljubljana, Slovenia. primoz.kotnik @ mf.uni-lj.si
Debeljak
Marusa
M
Avbelj
Magdalena
M
Hovnik
Tinka
T
Ursic Bratina
Natasa
N
Krzisnik
Ciril
C
Battelino
Tadej
T
D000483:Alleles; D016009:Chi-Square Distribution; D004247:DNA / Q000737:chemistry / Q000235:genetics; D003922:Diabetes Mellitus, Type 1 / Q000150:complications* / Q000235:genetics* / Q000276:immunology; D005260:Female; D014644:Genetic Variation; D050031:Hashimoto Disease / Q000150:complications* / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D013954:Thyroglobulin / Q000235:genetics*; D055815:Young Adult
D004247:DNA; D013954:Thyroglobulin