A novel functional gene selection method provides a systematic view of cell migration.
Cell migration is a central process that is essential for embryonic development, wound repair, inflammatory response, homeostasis and tumor metastasis. A method of genome-wide selection based on the gain-of-function has been devised to identify novel cell migration-promoting genes in cultured cells. After the introduction of the retroviral mouse brain cDNA library into NIH3T3 mouse fibroblast cells, migration-promoted cells were selected by a three-dimensional migration assay using cell culture inserts. After five rounds of enrichment, cDNAs were retrieved from the cells that passed the selection processes. Cell migration-promoting activity was confirmed by independent migration assays for the retrieved cDNAs. Multiple cell migration-promoting genes were successfully isolated by this method. The genes identified can be used to gain a systematic view of cell migration. The gain-of-function selection method described here can be combined with RNAi-mediated loss-of-function screen or selection to be a more powerful tool for the systems biology research of cell migration.
Suk
Kyoungho
K
Department of Pharmacology, Brain Science and Engineering Institute, Kyungpook National University School of Medicine, Joong-gu, Daegu, Korea. ksuk@knu.ac.kr
D000818:Animals; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D015723:Gene Library; D006801:Humans; D051379:Mice; D049490:Systems Biology / Q000379:methods*