Binding of Norovirus virus-like particles (VLPs) to human intestinal Caco-2 cells and the suppressive effect of pasteurized bovine colostrum on this VLP binding.
Noroviruses (NoVs), which cannot be grown in cell culture, are a major infectious agent of gastroenteritis. An in vitro assay system was established for the evaluation of NoV binding to enterocytes using virus-like particles (VLPs) produced in a baculovirus system expressing a NoV VP1 capsid protein. After confirmation of the purity by MS analysis, VLPs were incubated with human intestinal Caco-2 cells. NoV VLPs were detected clearly by confocal laser microscopy only on a certain population of Caco-2 cells, and were semi-quantified by immunoblotting of cell lysates. Then the suppressive effect of pasteurized bovine colostrum was analyzed on the VLP binding to Caco-2 cells by immunoblotting. The colostrum reduced VLP binding in a dose-dependent manner, at about 50% suppression with 12.5 microg of the colostral proteins. Furthermore, the colostrum contained IgG antibodies reacting to VLPs, suggesting that cross-reactive antibodies in the bovine colostrums block human NoV binding to intestinal cells.
Murakami
Kosuke
K
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, Japan.
Suzuki
Sayaka
S
Aoki
Naohito
N
Okajima
Tetsuya
T
Nadano
Daita
D
Uchida
Kenji
K
Yamashita
Kousaku
K
Oka
Tomoichiro
T
Katayama
Kazuhiko
K
Takeda
Naokazu
N
Matsuda
Tsukasa
T
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001681:Biological Assay; D018938:Caco-2 Cells; D036022:Capsid Proteins / Q000276:immunology*; D002417:Cattle; D003126:Colostrum / Q000276:immunology*; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D029322:Norovirus / Q000276:immunology*; D011247:Pregnancy; D014771:Virion / Q000276:immunology*; D053585:Virus Attachment
D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G