Identification of MyD88 as a novel target of miR-155, involved in negative regulation of Helicobacter pylori-induced inflammation.
MicroRNA-155 (miR-155) has been implicated as a central regulator of the immune system. We have previously reported that miR-155 negatively regulates Helicobacter pylori (H. pylori)-induced inflammation, but the molecular mechanism of miR-155 regulating the inflammation is not fully clear. Here, we identified myeloid differentiation protein 88 (MyD88) as a target gene of miR-155, and found that miR-155 decreased MyD88 expression at the protein but not the mRNA message level, suggesting that the miR-155-mediated inhibition is a post-transcriptional event. Furthermore, the overexpression of miR-155 led to significantly reduced IL-8 production induced by H. pylori infection. Thus, we have demonstrated that miR-155 can negatively regulate inflammation by targeting a key adaptor molecule MyD88 in inflammatory pathways.
Tang
Bin
B
Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing, China.
Xiao
Bin
B
Liu
Zhen
Z
Li
Na
N
Zhu
En-Dong
ED
Li
Bo-Sheng
BS
Xie
Qing-Hua
QH
Zhuang
Yuan
Y
Zou
Quan-Ming
QM
Mao
Xu-Hu
XH
D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D015536:Down-Regulation; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000382:microbiology; D016209:Interleukin-8 / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D053594:Myeloid Differentiation Factor 88 / Q000096:biosynthesis / Q000235:genetics*; D014176:Protein Biosynthesis
D016209:Interleukin-8; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D053594:Myeloid Differentiation Factor 88