Dynamic morphologies of pollen plastids visualised by vegetative-specific FtsZ1-GFP in Arabidopsis thaliana.
The behaviour and multiplication of pollen plastids have remained elusive despite their crucial involvement in cytoplasmic inheritance. Here, we present live images of plastids in pollen grains and growing tubes from transgenic Arabidopsis thaliana lines expressing stroma-localised FtsZ1-green-fluorescent protein fusion in a vegetative cell-specific manner. Vegetative cells in mature pollen contained a morphologically heterogeneous population of round to ellipsoidal plastids, whilst those in late-developing (maturing) pollen included plastids that could have one or two constriction sites. Furthermore, plastids in pollen tubes exhibited remarkable tubulation, stromule (stroma-filled tubule) extension, and back-and-forth movement along the direction of tube growth. Plastid division, which involves the FtsZ1 ring, was rarely observed in mature pollen grains.
Fujiwara
Makoto T
MT
RIKEN Nishina Center, Hirosawa 2-1, Wako, Saitama, 351-0198, Japan. mtf1@mac.com
Hashimoto
Haruki
H
Kazama
Yusuke
Y
Hirano
Tomonari
T
Yoshioka
Yasushi
Y
Aoki
Seishiro
S
Sato
Naoki
N
Itoh
Ryuuichi D
RD
Abe
Tomoko
T
D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000378:metabolism*; D018525:Germination; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D009928:Organ Specificity; D018087:Plastids / Q000378:metabolism* / Q000648:ultrastructure; D011058:Pollen / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
D029681:Arabidopsis Proteins; C095113:FTSZ protein, Arabidopsis; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins