[Epidemiological investigation on natural infection of different canine breeds with Borna disease virus in Ili, China].
OBJECTIVE
To investigate the epidemiological pattern of Borna disease virus (BDV) among different canine breeds in Ili, China, and to analyze its potential phylogeny.


METHODS
BDV p24 RNA fragments were detected from peripheral blood mononuclear cells (PBMCs) of canine by modified nested RT-PCR (nRT-PCR). Possible false positives were excluded by determination of both BDV p40 RNA fragments and PMD19 plasmid standards. Analysis were performed on genetic sequence, homologous comparison, amino acid sequence and phylogeny after p24 positive products were validated.


RESULTS
BDV p24 RNA fragments were found only in Kazakh Tobet (a shepherd dog) in 8 breeds of 150 cases and their overall positive rate was 11.0% (10/91). Compared with the strain of He/80 from horse and that of S6 from sheep in Germany, the homologous similarities of Kazakh Tobet was 99.2% and 95.7%, and that of amino acid as 100% and 89.3%, respectively. The kinship of Kazakh Tobet was close to He/80 and next to S6.


CONCLUSIONS
There was potential natural BDV infection in Kazakh Tobet in Ili, and its endemic strain was concerned with He/80 infecting Ili horse and S6 of German Merino sheep introduced into the region from Germany.
Zhan
Qun-ling
QL
Department of Neurology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Zhang
Ying-ying
YY
Xu
Ming-ming
MM
Hu
Yong-bo
YB
Wu
Xiu-ling
XL
Yu
Jian-ping
JP
Chen
Xiao
X
Zhu
Dan
D
Yang
De-yu
DY
Xie
Peng
P
D000595:Amino Acid Sequence; D000818:Animals; D001890:Borna Disease / Q000453:epidemiology* / Q000821:virology; D001891:Borna disease virus / Q000235:genetics / Q000302:isolation & purification*; D002681:China / Q000453:epidemiology; D004285:Dogs / Q000821:virology*; D005858:Germany / Q000453:epidemiology; D006734:Horse Diseases / Q000453:epidemiology / Q000821:virology; D006736:Horses / Q000821:virology; D007963:Leukocytes, Mononuclear / Q000821:virology; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D012367:RNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep / Q000821:virology; D012757:Sheep Diseases / Q000453:epidemiology / Q000821:virology; D013045:Species Specificity
D012367:RNA, Viral