title: Identification of osteoconductive and biodegradable polymers from a combinatorial polymer library.
abstract: Combinatorial polymer syntheses are now being utilized to create libraries of materials with potential utility for a wide variety of biomedical applications. We recently developed a library of photopolymerizable and biodegradable poly(beta-amino ester)s (PBAEs) that possess a range of tunable properties. In this study, the PBAE library was assessed for candidate materials that met design criteria (e.g., physical properties such as degradation and mechanical strength and in vitro cell viability and osteoconductive behavior) for scaffolding in mineralized tissue repair. The most promising candidate, A6, was then processed into three-dimensional porous scaffolds and implanted subcutaneously and only presented a mild inflammatory response. The scaffolds were then implanted intramuscularly and into a critical-sized cranial defect either alone or loaded with bone morphogenetic protein-2 (BMP-2). The samples in both locations displayed mineralized tissue formation in the presence of BMP-2, as evident through radiographs, micro-computed tomography, and histology, whereas samples without BMP-2 showed minimal or no mineralized tissue. These results illustrate a process to identify a candidate scaffolding material from a combinatorial polymer library, and specifically for the identification of an osteoconductive scaffold with osteoinductive properties via the inclusion of a growth factor.
authors.affiliation: Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Darren M
authors.initials: DM
authors.lastname: Brey
authors.forename: Cindy
authors.initials: C
authors.lastname: Chung
authors.forename: Kurt D
authors.initials: KD
authors.lastname: Hankenson
authors.forename: Jonathon P
authors.initials: JP
authors.lastname: Garino
authors.forename: Jason A
authors.initials: JA
authors.lastname: Burdick
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002478:Cells, Cultured; D020650:Combinatorial Chemistry Techniques; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D015394:Molecular Structure; D010012:Osteogenesis / Q000502:physiology; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D054852:Small Molecule Libraries; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D011108:Polymers; D054852:Small Molecule Libraries