The use of the ccdB lethal gene for constructing a zero background vector in order to clone blunt-end PCR products.
Hu
Li-Ling
LL
Key Lab of Ministry of Health for Hormone and Development, Tianjin Medical University Institute of Endocrinology, Tianjin 300070, China.
Zhang
Shan-Shan
SS
Li
Xiao-Xia
XX
Wang
Bao-Li
BL
D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005804:Genes, Lethal; D005822:Genetic Vectors / Q000235:genetics*; D016133:Polymerase Chain Reaction
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C068356:CcdB protein, Plasmid F; D004247:DNA