A search for hepatoprotective activity of fruit extract of Mangifera indica L. against oxidative stress cytotoxicity.
Mango (Mangifera indica L.) and their components are commonly used in folk medicine for many curative effects. The protective effects of different concentrations of aqueous extract of Mangifera indica L. fruit (Mango Extract) (20, 50 and 100 microg/ml) and also gallic acid (100 microM) as a pure compound in the extract were examined against oxidative stress toxicity induced by cumene hydroperoxide (CHP) in isolated rat hepatocytes. The extracts and gallic acid (100 microM) protected the hepatocyte against all oxidative stress markers including cell lysis, ROS generation, lipid peroxidation, glutathione depletion, mitochondrial membrane potential decrease, lysosomal membrane oxidative damage and cellular proteolysis. Mango Extracts (20, 50 and 100 microg/ml) were more effective than gallic acid (100 microM) in protecting hepatocytes against CHP induced lipid peroxidation. On the other hand gallic acid (100 microM) acted more effective than Mango Extracts (20, 50 and 100 microg/ml) at preventing lysosomal membrane damage. In addition H(2)O(2) scavenging effect of all extracts were determined in hepatocytes and compared with gallic acid (100 microM). There were no significance differences (P<0.05) between all plant extracts and gallic acid (100 microM) in H(2)O(2) scavenging activity. These results suggest a hepatoprotective role for Mango Extract against liver injury associated with oxidative stress.
Pourahmad
Jalal
J
Faculty of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran. j.pourahmadjaktaji@utoronto.ca
Eskandari
Mohammad Reza
MR
Shakibaei
Rashin
R
Kamalinejad
Mohammad
M
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001555:Benzene Derivatives; D015415:Biomarkers / Q000378:metabolism; D005638:Fruit; D005707:Gallic Acid / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006861:Hydrogen Peroxide; D007425:Intracellular Membranes / Q000187:drug effects; D008247:Lysosomes / Q000187:drug effects; D008297:Male; D031022:Mangifera / Q000737:chemistry*; D016877:Oxidants; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D001555:Benzene Derivatives; D015415:Biomarkers; D016877:Oxidants; D010936:Plant Extracts; D020011:Protective Agents; D005707:Gallic Acid; D006861:Hydrogen Peroxide; C007164:cumene hydroperoxide