Antimutagenic and anticarcinogenic effect of methanol extracts of Petasites japonicus Maxim leaves.
The methanol extract from the leaves of Petasites japonicus Maxim (PJ) was studied for its (anti-)mutagenic effect with the SOS chromotest and reverse mutation assay. The (anti-)carcinogenic effects were evaluated by the cytotoxicity on human cancer line cells and by the function and the expression of gap junctions in rat liver epithelial cell. PJ extracts significantly decreased spontaneous beta-galactosidase activity and beta-galactosidase activity induced by a mutagen, ICR, in Salmonella (S.) typhimurium TA 1535/pSK 1002. All doses of the extract (0.08-100 mg/plate) decreased the reversion frequency induced by benzo (alpha)pyrene (BaP) in S. typhimurium TA 98. It decreased not only the spontaneous reversion frequency but also that induced by BaP in S. typhimurium TA 100. PJ extract showed greater cytotoxic effects on human stomach, colon and uterus cancer cells than on other cancer cell types and normal rat liver epithelial cells. Dye transfers though gap junctions were significantly increased by PJ extracts at concentrations greater than 200 microg/mL and the inhibition of dye transfer by 12-O-tetradecanoylphorobol-13-acetate (TPA) was obstructed in all concentrations of PJ. PJ significantly increased the numbers of gap junction protein connexin 43, and increased the protein expression decreased by TPA in a dose-dependent manner. Based on these findings, PJ is suggested to contain antimutagenic and anticarcionogenic compounds.
Kang
Hwan-Goo
HG
National Veterinary Research and Quarantine Service, Anyang, Korea.
Jeong
Sang-Hee
SH
Cho
Joon-Hyoung
JH
D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D005562:Formazans / Q000737:chemistry; D017629:Gap Junctions / Q000378:metabolism*; D006801:Humans; D009152:Mutagenicity Tests; D036901:Petasites / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism; D051381:Rats; D013778:Tetrazolium Salts / Q000737:chemistry
D005562:Formazans; D010936:Plant Extracts; D013778:Tetrazolium Salts; C079782:MTT formazan