title: A flow cytometry-based assay for measuring invasion of red blood cells by Plasmodium falciparum.
abstract: Variability in the ability of the malaria parasite Plasmodium falciparum to invade human erythrocytes is postulated to be an important determinant of disease severity. Both the parasite multiplication rate and erythrocyte selectivity are important parameters that underlie such variable invasion. We have established a flow cytometry-based method for simultaneously calculating both the parasitemia and the number of multiply-infected erythrocytes. Staining with the DNA-specific dye SYBR Green I allows quantitation of parasite invasion at the ring stage of parasite development. We discuss in vitro and in vivo applications and limitations of this method in relation to the study of parasite invasion.
authors.affiliation: Harvard School of Public Health, Boston, Massachusetts, USA.
authors.forename: Amy K
authors.initials: AK
authors.lastname: Bei
authors.forename: Tiffany M
authors.initials: TM
authors.lastname: Desimone
authors.forename: Aida S
authors.initials: AS
authors.lastname: Badiane
authors.forename: Ambroise D
authors.initials: AD
authors.lastname: Ahouidi
authors.forename: Tandakha
authors.initials: T
authors.lastname: Dieye
authors.forename: Daouda
authors.initials: D
authors.lastname: Ndiaye
authors.forename: Ousmane
authors.initials: O
authors.lastname: Sarr
authors.forename: Omar
authors.initials: O
authors.lastname: Ndir
authors.forename: Souleymane
authors.initials: S
authors.lastname: Mboup
authors.forename: Manoj T
authors.initials: MT
authors.lastname: Duraisingh
mesh_terms: D052160:Benzothiazoles; D003959:Diamines; D004912:Erythrocytes / Q000469:parasitology*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D006790:Host-Parasite Interactions; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology*; D008853:Microscopy / Q000379:methods; D009930:Organic Chemicals; D018512:Parasitemia / Q000175:diagnosis; D010963:Plasmodium falciparum / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology; D011804:Quinolines; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I