title: Kinetic and conformational studies of adenosine deaminase upon interaction with oxazepam and lorazepam.
abstract: Oxazepam and lorazepam inhibit the adenosine deaminase (ADA) differently. In the case of lorazepam temperature increment causes an increase in the inhibition potency whereas higher temperature reduces the inhibitory effect of oxazepam; which proposes the overall profounder structural changes in the case of lorazepam relative to those caused by oxazepam.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. moosavi@ibb.ut.ac.ir
authors.forename: A A
authors.initials: AA
authors.lastname: Moosavi-Movahedi
authors.forename: H
authors.initials: H
authors.lastname: Sepassi Tehrani
authors.forename: M
authors.initials: M
authors.lastname: Amanlou
authors.forename: M N
authors.initials: MN
authors.lastname: Soltani Rad
authors.forename: G H
authors.initials: GH
authors.lastname: Hakimelahi
authors.forename: F-Y
authors.initials: FY
authors.lastname: Tsai
authors.forename: G
authors.initials: G
authors.lastname: Ataie
authors.forename: A A
authors.initials: AA
authors.lastname: Saboury
authors.forename: F
authors.initials: F
authors.lastname: Ahmad
authors.forename: A
authors.initials: A
authors.lastname: Khalafi-Nezhad
authors.forename: N
authors.initials: N
authors.lastname: Poursasan
authors.forename: A
authors.initials: A
authors.lastname: Sharifizadeh
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000243:Adenosine Deaminase / Q000737:chemistry* / Q000378:metabolism*; D058892:Adenosine Deaminase Inhibitors; D000818:Animals; D014151:Anti-Anxiety Agents / Q000378:metabolism; D000927:Anticonvulsants / Q000378:metabolism; D002417:Cattle; D002942:Circular Dichroism; D003198:Computer Simulation; D004791:Enzyme Inhibitors / Q000378:metabolism*; D006993:Hypnotics and Sedatives / Q000378:metabolism; D007413:Intestinal Mucosa / Q000201:enzymology; D007700:Kinetics; D008024:Ligands; D008140:Lorazepam / Q000378:metabolism*; D008958:Models, Molecular; D010076:Oxazepam / Q000378:metabolism*; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013696:Temperature
chemical_list: D058892:Adenosine Deaminase Inhibitors; D014151:Anti-Anxiety Agents; D000927:Anticonvulsants; D004791:Enzyme Inhibitors; D006993:Hypnotics and Sedatives; D008024:Ligands; D010076:Oxazepam; D000243:Adenosine Deaminase; D000241:Adenosine; D008140:Lorazepam