Polymorphisms in the 5' upstream region of the FSH receptor gene, and their association with superovulation traits in Chinese Holstein cows.
To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, variation in the bovine follicle stimulating hormone receptor (FSHR) gene was investigated using PCR-single-strand conformational (PCR-SSCP) and DNA sequencing. One single nucleotide polymorphism (SNP) of G-278A located in the 5' upstream region of bovine FSHR gene was found in 118 Chinese Holstein cows treated for superovulation. Two SNPs of G-278A (GU253337) and A-320T (rs43676359) were analyzed. In polymorphic locus -278, all cows without superovulation response were mutations with genotypes of CD and DD. Cows with CC genotype had a significant increase in the total number of ova (TNO) (P<0.01), and produced more transferable embryos (NTE) than those of the CD and DD genotypes (P<0.01). At this locus, the additive effects seemed to be highly significant (P<0.01) and allele C was associated with an increase in the TNO and NTE. These results indicated that the FSHR is a potential marker for superovulation response and can be used as a predictor for superovulation in Chinese Holstein cows.
Yang
Wu-Cai
WC
Key Lab of Education Ministry of China in Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, PR China.
Li
Shu-Jing
SJ
Tang
Ke-Qiong
KQ
Hua
Guo-Hua
GH
Zhang
Chun-Yan
CY
Yu
Jun-Na
JN
Han
Li
L
Yang
Li-Guo
LG
D000818:Animals; D002417:Cattle / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D040641:Quantitative Trait Loci / Q000235:genetics; D011962:Receptors, FSH / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013480:Superovulation / Q000235:genetics*
D005819:Genetic Markers; D011962:Receptors, FSH