title: [Association between expression of chromogranin A and myocardial fibrosis in patients with dilated cardiomyopathy].
abstract: OBJECTIVE
  To observe the possible correlation between expression of chromogranin A (CGA) and myocardial fibrosis and investigate the potential role of CGA in the development of myocardial fibrosis in patients with dilated cardiomyopathy (DCM).
  
  
  METHODS
  Surgical myocardial specimen from 10 DCM patients underwent successful orthotopic cardiac transplantation, and 3 normal myocardial specimen from brain-dead organ donors were obtained. CGA-mRNA, COLI-mRNA, COLIII-mRNA and ADAMTS-1-mRNA were analyzed by real-time PCR. The location and expression of CGA were assessed by immunohistochemistry(INH)with anti-CGA antibody. The collagen specific picrosirius red staining was applied on transversal myocardial slides and the collagen volume fraction (CVF) was calculated. The correlation between CGA and CVF was analyzed.
  
  
  RESULTS
  Cytoplasmic expression of CGA assessed by INH showed large amount of strong positive granules densely arranged in the epicardial and endocardial myocardiocytes in DCM specimen while there was only few sparse granules in the normal myocardium (P < 0.05). CVF was significantly higher in DCM myocardial specimen than that in normal specimen (P < 0.001). CGA-mRNA was significantly correlated with COLI-mRNA (r = 0.729), COLIII-mRNA (r = 0.95) and ADAMTS-1-mRNA (r = 0.665, all P < 0.05). Moreover, collagen deposition location was almost identical with the strong positive expression location of CGA.
  
  
  CONCLUSIONS
  We demonstrated for the first time that the deposition of CGA was related with the myocardial fibrosis in DCM heart, therefore, CGA might play an important role by influencing myocardial remodeling and fibrosis in DCM patients.
authors.affiliation: Key Laboratory of Viral Heart Diseases, Ministry of Public Health, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Ye-qing
authors.initials: YQ
authors.lastname: Xie
authors.forename: Rui-zhen
authors.initials: RZ
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Zhao-hua
authors.initials: ZH
authors.lastname: Yang
authors.forename: Qi
authors.initials: Q
authors.lastname: Guo
authors.forename: Ying-zhen
authors.initials: YZ
authors.lastname: Yang
mesh_terms: D000328:Adult; D002311:Cardiomyopathy, Dilated / Q000378:metabolism* / Q000473:pathology*; D053379:Chromogranin A / Q000096:biosynthesis*; D005260:Female; D005355:Fibrosis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology*
chemical_list: D053379:Chromogranin A