[Study of methylation of promoter of EYA1 gene in microtia].
OBJECTIVE
To explore the methylation of CpG islands in promoter of eye absent gene 1 (EYA1) in microtia.


METHODS
The methylation of CpG islands in EYA1 gene in 64 microtias and 36 healthy controls were measured using the technique of matrix-assisted laser desorption/ionization-time of flight.


RESULTS
The methylation of CpG_Unit3 and CpG_Unit5 of EYA1 gene in microtia were 0.09258 +/- 0.033846 and 0.0922 +/- 0.02379, respectively, which were significantly lower than those in control.


CONCLUSIONS
Hypomethylation in microtia may be related to the pathogenesis of the disease.
Lin
Lin
L
Plastic Surgery Hospital, Chinese Academy of Medical Science, Beijing 100144, China.
Pan
Bo
B
Jiang
Hai-yue
HY
Zhuang
Hong-xing
HX
Zhao
Yan-yong
YY
Yang
Qing-hua
QH
He
Le-ren
LR
Han
Juan
J
Wang
Shu-jie
SJ
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D018899:CpG Islands; D019175:DNA Methylation; D004423:Ear / Q000002:abnormalities*; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; C104381:EYA1 protein, human; D017027:Protein Tyrosine Phosphatases