Reproducible pattern of microRNA in normal human skin.
MicroRNAs (miRNAs) regulate cell growth, differentiation and apoptosis via specific targeting of messenger RNA (mRNA). Aberrant mRNA expression contributes to pathological processes such as carcinogenesis. To take advantage of miRNA profiling in skin disease it is essential to investigate miRNA expression pattern in normal human skin. Here we investigated miRNA expression profiles from skin biopsies of 8 healthy volunteers taken from sun protected and mildly photo damaged skin using the modified protocol for miRNA extraction. We were able to show a constant pattern of miRNA expression between different individuals. We did not find any significant differences in miRNA expression between sun protected and mildly photodamaged skin. These results may be valuable for future design of studies on miRNA expression in skin disease.
Holst
Line Marie
LM
Department of Dermatology, Bispebjerg Hospital, Copenhagen University, Copenhagen, Denmark. lhol0034@bbh.regionh.dk
Kaczkowski
Bogumil
B
Gniadecki
Robert
R
D000368:Aged; D001706:Biopsy; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D046228:Microarray Analysis; D008875:Middle Aged; D015203:Reproducibility of Results; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D015595:Skin Aging; D014466:Ultraviolet Rays / Q000009:adverse effects
D035683:MicroRNAs