Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine.
The skin is potentially an excellent organ for vaccine delivery because of accessibility and the presence of immune cells. However, no simple and inexpensive cutaneous vaccination method is available. Micron-scale needles coated with DNA were tested as a simple, inexpensive device for skin delivery. Vaccination with a plasmid encoding hepatitis C virus nonstructural 3/4A protein using microneedles effectively primed specific cytotoxic T lymphocytes (CTLs). Importantly, the minimally invasive microneedles were as efficient in priming CTLs as more complicated or invasive delivery techniques, such as gene gun and hypodermic needles. Thus, microneedles may offer a promising technology for DNA vaccination.
Gill
H S
HS
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Söderholm
J
J
Prausnitz
M R
MR
Sällberg
M
M
D000279:Administration, Cutaneous; D000818:Animals; D016174:Hepacivirus / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008845:Microinjections; D009339:Needles; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines