title: [Identification and characterization of ovarian cancer stem-like cells from primary tumor.].
abstract: OBJECTIVE
  To study whether the primary ovarian cancer cells containing cancer stem cells and its characterization in serum-free culture condition.
  
  
  METHODS
  The primary cancer cells were isolated from one stage III, grade 2 serous adenocarcinoma tissue. Cells were cultured in serum-free culture system supplemented with epidermal growth factor, basic fibroblast growth factor, leukemia inhibitory factor and insulin, or standard serum-containing system. Methyl thiazolyl tetrazolium assay, quantitative PCR analysis, flow-cytometric analysis and xenograft experiments in vivo were performed.
  
  
  RESULTS
  The primary cancer cells could maintain and form cell sphere in serum-free culture system. These cells had the properties of self-renewal, overexpression of stem cell marker genes Nanog, Oct-4, Sox-2, nestin, ABCG2, CD(133) and CD(117). By contrast with the differentiated cells under standard serum-containing culture conditions, these sphere-forming cells were more resistant to cisplatin and paclitaxel after treated 48 and 72 hours (61% vs. 31%, 73% vs. 29%, P < 0.05). With Hoechst 33342 exclusion assay, only 21.83% of sphere-forming cells were positive with the dye, compared with 83.04% positive cells in differentiated cells (P < 0.01). Only 500 sphere-forming cells resulted subcutaneous xenograft tumors. All of these xenografts were categorized as serous adenocarcinomas, overexpression of CA(125) and cytokeratin-7 which were original tumor phenotype of ovarian cancer.
  
  
  CONCLUSIONS
  The sphere-forming cells isolated from primary ovarian cancer tissues have the characterization of cancer stem cell and may be a more reliable model system for understanding the biology of primary human tumors.
authors.affiliation: Department of Gynecologic Oncology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Lai
authors.forename: Te
authors.initials: T
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Cheng
mesh_terms: D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D018284:Cystadenocarcinoma, Serous; D005260:Female; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D010051:Ovarian Neoplasms
chemical_list: D050814:Octamer Transcription Factor-3; D002945:Cisplatin