title: Synthesizing a novel genetic sequential logic circuit: a push-on push-off switch.
abstract: Design and synthesis of basic functional circuits are the fundamental tasks of synthetic biologists. Before it is possible to engineer higher-order genetic networks that can perform complex functions, a toolkit of basic devices must be developed. Among those devices, sequential logic circuits are expected to be the foundation of the genetic information-processing systems. In this study, we report the design and construction of a genetic sequential logic circuit in Escherichia coli. It can generate different outputs in response to the same input signal on the basis of its internal state, and 'memorize' the output. The circuit is composed of two parts: (1) a bistable switch memory module and (2) a double-repressed promoter NOR gate module. The two modules were individually rationally designed, and they were coupled together by fine-tuning the interconnecting parts through directed evolution. After fine-tuning, the circuit could be repeatedly, alternatively triggered by the same input signal; it functions as a push-on push-off switch.
authors.affiliation: 2007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking University, Beijng, PR China.
authors.forename: Chunbo
authors.initials: C
authors.lastname: Lou
authors.forename: Xili
authors.initials: X
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Ni
authors.forename: Yiqi
authors.initials: Y
authors.lastname: Huang
authors.forename: Qiushi
authors.initials: Q
authors.lastname: Huang
authors.forename: Longwen
authors.initials: L
authors.lastname: Huang
authors.forename: Lingli
authors.initials: L
authors.lastname: Jiang
authors.forename: Dan
authors.initials: D
authors.lastname: Lu
authors.forename: Mingcong
authors.initials: M
authors.lastname: Wang
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Daizhuo
authors.initials: D
authors.lastname: Chen
authors.forename: Chongyi
authors.initials: C
authors.lastname: Chen
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Chen
authors.forename: Le
authors.initials: L
authors.lastname: Yang
authors.forename: Haisu
authors.initials: H
authors.lastname: Ma
authors.forename: Jianguo
authors.initials: J
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Ouyang
mesh_terms: D001665:Binding Sites; D003198:Computer Simulation; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects; D053263:Gene Regulatory Networks; D008957:Models, Genetic; D009154:Mutation / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism; D015398:Signal Transduction / Q000528:radiation effects; D014466:Ultraviolet Rays