title: Apple proanthocyanidins do not reduce the induction of preneoplastic lesions in the colon of rats associated with human microbiota.
abstract: Since the gut microbiota metabolizes various dietary constituents unabsorbed by the small intestine and modulates colon function, it plays an essential role in colon carcinogenesis. First, we have developed a model of human microbiota-associated rats (HMA), fed a human-type diet and injected with 1-2,dimethylhydrazine (DMH). We observed that the number and size of DMH-induced aberrant crypt foci (ACF) were significantly higher in HMA rats than in germ-free or conventional rats. Second, we used this model to assess the protective effect of an apple proanthocyanidin-rich extract (APE) on colon carcinogenesis. In this model, ACF number and multiplicity were not reduced by APE at 0.001% and 0.01% in drinking water. They were higher with APE 0.1% than with APE 0.01%. Therefore, the cross-talk between human microbiota and the colon epithelium should be taken into account in carcinogenesis models. Moreover, attention should be paid prior to using proanthocyanidin extracts as dietary supplements for humans.
authors.affiliation: INRA, UR910 Unite d'Ecologie et Physiologie du Systeme Digestif, Institut National de la Recherche Agronomique (INRA), Jouy-en-Josas, France.
authors.forename: Evelyne F
authors.initials: EF
authors.lastname: Lhoste
authors.forename: Aurélia
authors.initials: A
authors.lastname: Bruneau
authors.forename: Martine
authors.initials: M
authors.lastname: Bensaada
authors.forename: Claire
authors.initials: C
authors.lastname: Cherbuy
authors.forename: Catherine
authors.initials: C
authors.lastname: Philippe
authors.forename: Sandrine
authors.initials: S
authors.lastname: Bruel
authors.forename: Malène
authors.initials: M
authors.lastname: Sutren
authors.forename: Sylvie
authors.initials: S
authors.lastname: Rabot
authors.forename: Sylvain
authors.initials: S
authors.lastname: Guyot
authors.forename: Pierre-Henri
authors.initials: PH
authors.lastname: Duée
authors.forename: Paule
authors.initials: P
authors.lastname: Latino-Martel
mesh_terms: D000818:Animals; D003106:Colon / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology*; D003110:Colonic Neoplasms / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D006801:Humans; D008297:Male; D027845:Malus / Q000737:chemistry*; D054892:Metagenome; D010936:Plant Extracts / Q000494:pharmacology*; D011230:Precancerous Conditions / Q000382:microbiology; D044945:Proanthocyanidins / Q000494:pharmacology*; D051381:Rats; D013047:Specific Pathogen-Free Organisms
chemical_list: D010936:Plant Extracts; D044945:Proanthocyanidins