title: Bacterial biodiversity and dynamics during malolactic fermentation of Tempranillo wines as determined by a culture-independent method (PCR-DGGE).
abstract: The bacterial population during malolactic fermentation of Tempranillo wine was studied using the polymerase chain reaction-denaturing gradient gel electrophoresis, a culture-independent method successfully used for identification and monitoring of bacterial population in different habitats included food fermentations. The results showed that Oenococcus oeni was the predominant species in the malolactic fermentation of Tempranillo wines, although the presence of Gluconobacter oxydans, Asaia siamensis, Serratia sp., and Enterobacter sp. was also observed. These results were partly coincidental with those obtained from a culture-dependent method, using a selective medium. Therefore, it may be concluded that for a more complete knowledge of the bacterial community present during malolactic fermentation of Tempranillo wine, an approach that combines a culture-independent method and a culture-dependent method would be advisable.
authors.affiliation: Departamento de Química Analítica y Tecnología de Alimentos, Universidad de Castilla-La Mancha, Avda. Carlos III s/n, 45071, Toledo, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Ruiz
authors.forename: Susana
authors.initials: S
authors.lastname: Seseña
authors.forename: Pedro Miguel
authors.initials: PM
authors.lastname: Izquierdo
authors.forename: María Llanos
authors.initials: ML
authors.lastname: Palop
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D044822:Biodiversity; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D056585:Oenococcus / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D014920:Wine / Q000382:microbiology*