Fluorescence diffuse optical tomography for free-space and multifluorophore studies.
We present two major advances in preclinical fluorescence-enhanced diffuse optical tomography (fDOT) system and assess its performance. It is now possible to perform experiments without adaptation liquid or a glass plate over the animal, and our system is equipped with a filter wheel in order to discriminate two injected fluorophores. Evaluation carried out on characterization phantoms and in vivo on mice demonstrates enriched use of the system for biological studies on small animals.
Koenig
Anne
A
CEA-LETI, Minatec, Grenoble, France. anne.koenig@cea.fr
Hervé
Lionel
L
Gonon
Georges
G
Josserand
Véronique
V
Berger
Michel
M
Dinten
Jean-Marc
JM
Boutet
Jérome
J
Peltié
Philippe
P
Coll
Jean-Luc
JL
Rizo
Philippe
P
D000818:Animals; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007834:Lasers; D008325:Mammary Neoplasms, Experimental / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D041622:Tomography, Optical / Q000379:methods*; D051598:Whole Body Imaging / Q000379:methods
D005456:Fluorescent Dyes