title: Fungal growth inhibition of regenerated cellulose nanofibrous membranes containing Quillaja saponin.
abstract: Antifungal properties were introduced in nonwoven regenerated cellulose (RC) nanofibrous membrane using Quillaja saponin. To generate cellulose membranes, deacetylation of electrospun cellulose acetate (CA) nanofibrous membranes was performed using 0.05 M NaOH and ethanol for membranes both loaded and unloaded with Quillaja saponin. Chemical and physical properties of nonwoven CA and RC nanofibrous membrane were characterized using scanning electron microscopy, attenuated total reflectance-Fourier transform infrared spectroscopy, differential scanning calorimetry, and tensile properties. Our results showed that saponin doping did not affect the morphology of the resulting fibers and that the membrane structure was maintained during deacetylation. The antifungal properties of saponin-loaded fabric were determined at 0 and 24 h against two household fungi, Penicillium roguefortii and Aspergillus ochraceus, and compared with control samples. Our findings show that after 24 h the saponin-loaded fabrics had spores kill of 80.4% and 53.6% for P. roguefortii and A. ochraceus, respectively. Fabric containing Quillaja saponin has potential for household applications and could be evaluated further for medical applications.
authors.affiliation: Cornell University, Ithaca, NY 14853-4401, USA.
authors.forename: Vivechana
authors.initials: V
authors.lastname: Dixit
authors.forename: Jagdish
authors.initials: J
authors.lastname: Tewari
authors.forename: S Kay
authors.initials: SK
authors.lastname: Obendorf
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000633:toxicity*; D002482:Cellulose / Q000737:chemistry / Q000648:ultrastructure; D005374:Filtration; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development; D006131:Growth Inhibitors / Q000737:chemistry / Q000633:toxicity*; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D031990:Quillaja / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D013172:Spores, Fungal / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D006131:Growth Inhibitors; D002482:Cellulose