title: Interaction of an anthracycline disaccharide with ctDNA: Investigation by spectroscopic technique and modeling studies.
abstract: This study was designed to examine the interaction of an anthracycline disaccharide, 4'-O-(beta-L-oleandrosyl) daunorubicin (DNR-D2), with calf thymus deoxyribonucleic acid (ctDNA) by UV-vis in combination with fluorescence spectroscopy and molecular modeling techniques under physiological conditions (Britton-Robinson buffer solutions, pH 7.4). By the analysis of UV-vis and fluorescence spectrum, it was observed that the binding mode between DNR-D2 and ctDNA might be intercalation, and fluorescence quenching mechanism of DNR-D2 by ctDNA was a static quenching type. Upon binding to ctDNA, the anthraquinone chromophore of DNR-D2 could slide into the C-G rich region of ctDNA. Hydrogen bonding forces may play an essential role in the binding of DNR-D2 to ctDNA. Furthermore, the results obtained from computational modeling corroborated the experimental results obtained from spectroscopic investigations. These studies are valuable for a better understanding the datailed mode of DNR-D2-DNA interaction, which should be important in deeper insight into the therapeutic efficiency of DNR-D2.
authors.affiliation: School of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China. yanlu2001@sohu.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Juan
authors.initials: J
authors.lastname: Lv
authors.forename: Guisheng
authors.initials: G
authors.lastname: Zhang
authors.forename: Gongke
authors.initials: G
authors.lastname: Wang
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: C550479:4'-O-(oleandrosyl)daunorubicin; D004187:Disaccharides; D004247:DNA; C427203:calf thymus DNA; D003630:Daunorubicin