title: Glutathione protects Lactobacillus sanfranciscensis against freeze-thawing, freeze-drying, and cold treatment.
abstract: Lactobacillus sanfranciscensis DSM20451 cells containing glutathione (GSH) displayed significantly higher resistance against cold stress induced by freeze-drying, freeze-thawing, and 4 degrees C cold treatment than those without GSH. Cells containing GSH were capable of maintaining their membrane structure intact when exposed to freeze-thawing. In addition, cells containing GSH showed a higher proportion of unsaturated fatty acids in cell membranes upon long-term cold treatment. Subsequent studies revealed that the protective role of GSH against cryodamage of the cell membrane is partly due to preventing peroxidation of membrane fatty acids and protecting Na(+),K(+)-ATPase. Intracellular accumulation of GSH enhanced the survival and the biotechnological performance of L. sanfranciscensis, suggesting that the robustness of starters for sourdough fermentation can be improved by selecting GSH-accumulating strains. Moreover, the results of this study may represent a further example of mechanisms for stress responses in lactic acid bacteria.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Jiangsu, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.forename: Guo-Cheng
authors.initials: GC
authors.lastname: Du
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xian-Yan
authors.initials: XY
authors.lastname: Liao
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D003080:Cold Temperature; D005227:Fatty Acids / Q000378:metabolism; D005285:Fermentation; D005612:Freeze Drying; D005615:Freezing; D005978:Glutathione / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism* / Q000502:physiology; D015227:Lipid Peroxidation
chemical_list: D005227:Fatty Acids; D005978:Glutathione