Sterol effect on interaction between amphidinol 3 and liposomal membrane as evidenced by surface plasmon resonance.
The affinity of amphidinol 3 (AM3) to phospholipid membranes in the presence and absence of sterol was examined by surface plasmon resonance (SPR) experiments. The results showed that AM3 has 1000 and 5300 times higher affinity for cholesterol- and ergosterol-containing liposomes, respectively, than those without sterol. The two-state reaction model well reproduced the sensor grams, which indicated that the interaction is composed of two steps, which correspond to binding to the membrane and internalization to form stable complexes.
Swasono
Respati T
RT
Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
Mouri
Ryota
R
Morsy
Nagy
N
Matsumori
Nobuaki
N
Oishi
Tohru
T
Murata
Michio
M
D000475:Alkenes / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D004875:Ergosterol / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D011714:Pyrans / Q000737:chemistry*; D013261:Sterols / Q000737:chemistry; D020349:Surface Plasmon Resonance; D000095722:Amphidinols
D000475:Alkenes; D002784:Cholesterol; D004875:Ergosterol; D008081:Liposomes; D011714:Pyrans; D013261:Sterols; C438973:amphidinol 3; D000095722:Amphidinols