Fluorescence enhancement of the silver nanoparticales--curcumin-cetyltrimethylammonium bromide-nucleic acids system and its analytical application.
It is found that silver nanoparticles (AgNPs) can further enhance the fluorescence intensity of curcumin (CU)-cetyltrimethylammonium bromide (CTAB)-nucleic acids and improve its anti-photobleaching activity. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentration of nucleic acids in the range of 2.0 x 10(-8)-1.0 x 10(-6) g mL(-1) for fish sperm DNA (fsDNA), 2.0 x 10(-8)-1.0 x 10(-6) g mL(-1) for calf thymus DNA (ctDNA), 1.0 x 10(-8)-1.0 x 10(-6) g mL(-1) for yeast RNA (yRNA), and their detection limits (S/N = 3) are 8.0 ng mL(-1), 10.5 ng mL(-1) and 5.8 ng mL(-1), respectively. This method is used for determining the concentration of DNA in actual sample with satisfactory results. The interaction mechanism is also studied.
Zhou
Haiping
H
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China.
Wu
Xia
X
Xu
Wei
W
Yang
Jinghe
J
Yang
Qiuxia
Q
D001965:Bromides / Q000737:chemistry*; D002021:Buffers; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D003474:Curcumin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry; D038761:Photobleaching; D012542:Scattering, Radiation; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry
D001965:Bromides; D002021:Buffers; D002593:Cetrimonium Compounds; D009696:Nucleic Acids; D013501:Surface-Active Agents; D012834:Silver; D003474:Curcumin; D000077286:Cetrimonium