[Lycopene and beta-carotene content in tomato analyzed by the second harmonic].
Lycopene and beta-carotene are two important nutritional components in tomato. The main Raman spectrum group of lycopene and beta-carotene abundant in tomato is identical and difficult to be distinguished through fundamental frequency. With excitation wavelength of 514.5 nm, the excited light was just present in the half width range of the main absorption bands of Lycopene and beta-carotene, so the resonance Raman effect can occur. Based on resonance Raman spectra, by on-body measuring the second harmonic of stretching vibration of carbon-carbon conjugated double bond in lycopene and beta-carotene, the content of lycopene and beta-carotene can be obtained according to the integrated intensity of each component calculated by software. And this provides a method for on-body determining the content of the components with the homologous group.
Ouyang
Shun-Li
SL
State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, China.
Zhou
Mi
M
Cao
Biao
B
Men
Zhi-Wei
ZW
Gao
Shu-Qin
SQ
Li
Zuo-Wei
ZW
Lu
Guo-Hui
GH
Yang
Jian-Ge
JG
D002338:Carotenoids / Q000032:analysis*; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D019207:beta Carotene / Q000032:analysis*
D019207:beta Carotene; D002338:Carotenoids; D000077276:Lycopene