CRP-induced levels of oxidative stress are higher in brain than aortic endothelial cells.
C-reactive protein (CRP) has been demonstrated to induce blood-brain barrier disruption (BBB) involving NAD(P)H-oxidase dependent oxidative stress. It is unclear why CRP affects the BBB and not other vascular beds following stroke. Therefore we examined CRP receptor and NAD(P)H-oxidase expression levels in bovine brain- (BEC) and aortic endothelial cells. Dichlorodihydrofluorescein measurements revealed significantly higher CRP-induced reactive oxygen species (ROS) levels in BEC. Protein expression of the CRP-receptors CD16, CD32 and of the NAD(P)H-oxidase subunit p22phox were also significantly higher in BEC. In conclusion BEC show a higher vulnerability to CRP due to increased levels of CRP receptors and the NAD(P)H-oxidase.
Closhen
Dorothea
D
University Medical Center of the Johannes Gutenberg University Mainz, Institute of Physiology and Pathophysiology, Duesbergweg 6, Mainz, Germany.
Bender
Bianca
B
Luhmann
Heiko J
HJ
Kuhlmann
Christoph R W
CR
D000818:Animals; D001011:Aorta / Q000166:cytology*; D001921:Brain / Q000166:cytology*; D002097:C-Reactive Protein / Q000494:pharmacology*; D002417:Cattle; D042783:Endothelial Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D019255:NADPH Oxidases / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017452:Receptors, IgG / Q000378:metabolism
D017452:Receptors, IgG; D002097:C-Reactive Protein; D019255:NADPH Oxidases