ppGpp-mediated stationary phase induction of the genes encoded by horizontally acquired pathogenicity islands and cob/pdu locus in Salmonella enterica serovar Typhimurium.
Salmonella enterica is highly diverse in terms of genome structure, which is at least partly due to the horizontal transfer of genetic elements from various sources. In this study, we examined the expression profiles of such genes in Salmonella Pathogenicity Islands (SPIs) and the cob/pdu locus, horizontally acquired large DNA segments, during growth under standard growth conditions. Transcripts from exponentially growing and early stationary phase Salmonellae were compared using various methods including cDNA microarray analysis. Nearly all genes encoded by SPIs and the cob/pdu locus were induced at the onset of the stationary phase in a stringent molecule ppGpp-dependent but stationary phase sigma, sigma38-independent manner. Although, it has been suggested that ppGpp acts in concert with DksA, we found the stationary phase induction of those SPI genes was not DksA dependent. It is suggested that ppGpp stimulates the expression of these stress-inducible genes encoded by horizontally acquired DNA, by itself or in concert with DksA.
Song
Miryoung
M
Research Center for Enteropathogenic Bacteria and Research Institute of Vibrio Infection, Gwangju, Republic of Korea.
Kim
Hyun-Ju
HJ
Ryu
Sangryeol
S
Yoon
Hyunjin
H
Yun
Jiae
J
Choy
Hyon E
HE
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D018929:Cell Culture Techniques; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D022761:Gene Transfer, Horizontal; D056426:Genetic Loci; D044404:Genomic Islands / Q000235:genetics*; D006159:Guanosine Tetraphosphate / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D008565:Membrane Proteins; C440638:SPI-2 protein, Salmonella; C419942:Spi1 protein, Salmonella; D006159:Guanosine Tetraphosphate