title: Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro.
abstract: We describe here a contribution of virus-induced actin tails and filopodia in transmission of Ectromelia virus (ECTV) infection in permissive cells detected by the immunofluorescence and confocal microscopy. Immunoblot analysis revealed profoundly decreased beta-actin levels during ECTV replicative cycle in the infected cells 24 hrs post infection (p.i.). These results provided a basis for the further analysis of ECTV motion in the infected cells as well as for impact of ECTV infection on the cytoskeletal proteins.
authors.affiliation: Division of Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Boratynska
authors.forename: L
authors.initials: L
authors.lastname: Martyniszyn
authors.forename: L
authors.initials: L
authors.lastname: Szulc
authors.forename: M
authors.initials: M
authors.lastname: Krzyzowska
authors.forename: J
authors.initials: J
authors.lastname: Szczepanowska
authors.forename: M G
authors.initials: MG
authors.lastname: Niemialtowski
mesh_terms: D000199:Actins / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D041702:BALB 3T3 Cells / Q000648:ultrastructure / Q000821:virology; D002522:Chlorocebus aethiops; D003598:Cytoskeletal Proteins / Q000378:metabolism / Q000648:ultrastructure; D004481:Ectromelia virus / Q000472:pathogenicity* / Q000502:physiology; D004482:Ectromelia, Infectious / Q000821:virology; D005528:Foot / Q000821:virology; D006367:HeLa Cells / Q000648:ultrastructure / Q000821:virology; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D014709:Vero Cells / Q000648:ultrastructure / Q000821:virology
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins