title: Impact of viral enhancin genes on potency of Lymantria dispar multiple nucleopolyhedrovirus in L. dispar following disruption of the peritrophic matrix.
abstract: Enhancins are metalloproteases found in many betabaculoviruses and several alphabaculoviruses, which enhance alphabaculovirus potency by degrading a protein component of the peritrophic matrix (PM), facilitating passage of virions through this structure. Earlier studies on betabaculovirus enhancins within heterologous systems suggested that enhancins facilitate virion binding to midgut cells. We compared the potency of wild-type Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) with that of single and double enhancin deletion viruses in L. dispar in the presence and absence of an intact PM. Compared to wild-type virus, the double enhancin deletion virus was 6-fold and 14-fold less potent, respectively, indicating that within this homologous system the LdMNPV enhancin genes have a function beyond PM degradation.
authors.affiliation: Department of Entomology, The Pennsylvania State University, University Park, PA 16802, USA. khoover@psu.edu
authors.forename: Kelli
authors.initials: K
authors.lastname: Hoover
authors.forename: Merideth A
authors.initials: MA
authors.lastname: Humphries
authors.forename: Alyssa R
authors.initials: AR
authors.lastname: Gendron
authors.forename: James M
authors.initials: JM
authors.lastname: Slavicek
mesh_terms: D000818:Animals; D041981:Gastrointestinal Tract / Q000821:virology; D017353:Gene Deletion; D005814:Genes, Viral / Q000502:physiology*; D045726:Metalloproteases / Q000235:genetics*; D009036:Moths / Q000821:virology*; D019251:Nucleocapsid / Q000235:genetics; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000472:pathogenicity; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins; D045726:Metalloproteases