Profiling DNA methylomes from microarray to genome-scale sequencing.
DNA cytosine methylation is a central epigenetic modification which plays critical roles in cellular processes including genome regulation, development and disease. Here, we review current and emerging microarray and next-generation sequencing based technologies that enhance our knowledge of DNA methylation profiling. Each methodology has limitations and their unique applications, and combinations of several modalities may help build the entire methylome. With advances on next-generation sequencing technologies, it is now possible to globally map the DNA cytosine methylation at single-base resolution, providing new insights into the regulation and dynamics of DNA methylation in genomes.
Huang
Yi-Wei
YW
The Ohio State University Comprehensive Cancer Center, Columbus, OH 43210, USA. Yi-wen.huang@osumc.edu
Huang
Tim H-M
TH
Wang
Li-Shu
LS
D000818:Animals; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*