title: Evidence for a novel cytoplasmic processing event in ribosome maturation in the sea urchin Paracentrotus lividus.
abstract: In this paper we demonstrate the existence of a cytoplasmic processing step, never before described, involving both the pre-ribosomal subunits in the sea urchin Paracentrotus lividus. Northern-blot hybridization, primer extension, S1 mapping experiments and in situ hybridizations allowed us to demonstrate that cytoplasmic processed particles are successively re-imported into the nucleus where maturation of their RNAs is completed prior to being exported to the cytoplasm. Our findings lead to the proposal of a new model of ribosome maturation and shuttling.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo, Università degli Studi di Palermo, V.le delle Scienze, Edificio 16, 90128, Palermo, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Bellavia
authors.forename: Rainer
authors.initials: R
authors.lastname: Barbieri
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D017403:In Situ Hybridization; D009865:Oocytes / Q000378:metabolism; D047328:Paracentrotus / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism*
chemical_list: D012322:RNA Precursors