title: Oncostatin M induces dendritic cell maturation and Th1 polarization.
abstract: Oncostatin M (OSM) is a pleiotropic cytokine and a member of the gp130/IL-6 cytokine family that has been found to be involved in both pro- and anti-inflammatory responses in cell-mediated immunity. Maturation of dendritic cells (DCs) is crucial for initiation of primary immune responses and is regulated by several stimuli. In this study, the role of OSM in the phenotypic and functional maturation of DCs was evaluated in vitro. Stimulation with OSM upregulated the expression of CD80, CD86, MHC class I and MHC class II and reduced the endocytic capacity of immature DCs. Moreover, OSM induced the allogeneic immunostimulatory capacity of DCs by stimulating the production of the Th1-promoting cytokine IL-12. OSM also increased the production of IFN-gamma by T cells in mixed-lymphocyte reactions, which would be expected to contribute to the Th1 polarization of the immune response. The expression of surface markers and cytokine production in DCs was mediated by both the MAPK and NF-kappaB pathways. Taken together, these results indicate that OSM may play a role in innate immunity and in acquired immunity by enhancing DCs maturation and promoting Th1 immune responses.
authors.affiliation: Department of Microbiology and Immunology and National Research Laboratory of Dendritic Cell Differentiation and Regulation, School of Medicine, Pusan National University, Beom-eo Ri, Mulgum Eop, Yangsan, Gyeongsangnam-do 626-770, South Korea.
authors.forename: In Duk
authors.initials: ID
authors.lastname: Jung
authors.forename: Kyung Tae
authors.initials: KT
authors.lastname: Noh
authors.forename: Chang-Min
authors.initials: CM
authors.lastname: Lee
authors.forename: Sung Hak
authors.initials: SH
authors.lastname: Chun
authors.forename: Soo Kyung
authors.initials: SK
authors.lastname: Jeong
authors.forename: Jin Wook
authors.initials: JW
authors.lastname: Park
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Park
authors.forename: Han Wool
authors.initials: HW
authors.lastname: Kim
authors.forename: Cheol-Heui
authors.initials: CH
authors.lastname: Yun
authors.forename: Yong Kyoo
authors.initials: YK
authors.lastname: Shin
authors.forename: Yeong-Min
authors.initials: YM
authors.lastname: Park
mesh_terms: D021581:Active Transport, Cell Nucleus; D056704:Adaptive Immunity; D000818:Animals; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D004789:Enzyme Activation; D007113:Immunity, Innate; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D020929:Mitogen-Activated Protein Kinase Kinases / Q000096:biosynthesis; D016328:NF-kappa B / Q000378:metabolism; D053683:Oncostatin M / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D053681:Oncostatin M Receptor beta Subunit / Q000096:biosynthesis; D018417:Th1 Cells / Q000276:immunology*
chemical_list: D016328:NF-kappa B; D053681:Oncostatin M Receptor beta Subunit; D053683:Oncostatin M; D016753:Interleukin-10; D018664:Interleukin-12; D020929:Mitogen-Activated Protein Kinase Kinases