Identification and characterization of CMTM4, a novel gene with inhibitory effects on HeLa cell growth through Inducing G2/M phase accumulation.
Human CMTM is a novel gene family consisting of CKLF and CMTM1-8. CMTM4 is the most conserved gene and has three RNA splicing forms designated as CMTM4-v1, -v2 and -v3, but in many types of tissue and cell lines, only CMTM4-v1 and -v2 could be detected. CMTM4-v2 is the full length cDNA product, which has been highly conserved during evolution. CMTM4-v1 and -v2 are broadly expressed in normal types of tissue. They are distributed on the cell membrane and across the cytoplasm in a speckled pattern. Overexpression of CMTM4-v1 and -v2 can inhibit HeLa cell growth via G2/M phase accumulation without inducing apoptosis. Therefore, CMTM4 might be an important gene involved in cell growth and cell cycle regulation.
Plate
Markus
M
Center for Human Disease Genomics, Department of Immunology, School of Basic Medical Science, Health Science Center, Peking University, Beijing, 100191, China.
Li
Ting
T
Wang
Yu
Y
Mo
Xiaoning
X
Zhang
Yingmei
Y
Ma
Dalong
D
Han
Wenling
W
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002455:Cell Division / Q000502:physiology*; D049109:Cell Proliferation; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D016195:G2 Phase / Q000502:physiology*; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D062792:MARVEL Domain-Containing Proteins; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014162:Transfection
C551726:CMTM4 protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins; D008565:Membrane Proteins; D020033:Protein Isoforms; D049452:Green Fluorescent Proteins