title: Kinetic change of oxidative stress in cerebrospinal fluid of mice infected with Angiostrongylus cantonensis.
abstract: The cerebrospinal fluid (CSF) of C57BL/6 mice infected with Angiostrongylus cantonensis was examined for kinetic changes in oxidative stress parameters, including reactive oxygen species (ROS), superoxide dismutase (SOD), catalase, malondialdehyde (MDA), 8-isoprostane, and 8-hydroxy-2'-deoxyguanosine (8-OHdG). The ROS increased gradually in the early stage of infection. During days 12-30 post-infection, the infected mice revealed ROS levels significantly higher than that in uninfected controls (P < 0.001). The ROS levels peaked at day 24 and then returned to that observed in uninfected controls at day 45 post-infection. The kinetics of MDA, 8-isoprostane, and 8-OHdG concentration changes observed in the CSF of the infected mice corresponded with kinetic changes in ROS levels. Thus, the excess ROS caused lipid peroxidation and DNA damage to cells in the central nervous system (CNS) of mice infected with A. cantonensis despite the increased antioxidant SOD and catalase enzyme activities during post-infection days 12-30. The oxidative stress in the CNS of C57BL/6 mice was apparently increased by diseases associated with A. cantonensis infection.
authors.affiliation: Department of Parasitology and Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, 80708Kaohsiung, Taiwan, Republic of China.
authors.forename: Li-Yu
authors.initials: LY
authors.lastname: Chung
authors.forename: Lian-Chen
authors.initials: LC
authors.lastname: Wang
authors.forename: Chun-Hsiang
authors.initials: CH
authors.lastname: Chen
authors.forename: Hsiao-Yi
authors.initials: HY
authors.lastname: Lin
authors.forename: Chuan-Min
authors.initials: CM
authors.lastname: Yen
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D017185:Angiostrongylus cantonensis; D000818:Animals; D002374:Catalase / Q000134:cerebrospinal fluid; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000134:cerebrospinal fluid; D015237:Dinoprost / Q000031:analogs & derivatives / Q000134:cerebrospinal fluid; D007700:Kinetics; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017206:Strongylida Infections / Q000134:cerebrospinal fluid*; D013482:Superoxide Dismutase / Q000134:cerebrospinal fluid
chemical_list: D017382:Reactive Oxygen Species; C075750:8-epi-prostaglandin F2alpha; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D002374:Catalase; D013482:Superoxide Dismutase; D003849:Deoxyguanosine