title: Evaluation of a modified microscopic direct diagnosis of dermatophytosis.
abstract: Here we present a modified protocol for dematophyte diagnosis, utilizing a simple centrifugation step to significantly decrease false-negative results of the original KOH direct microscopy-based technique. Although culture constitutes the gold-standard diagnosis, the time spent for results is a limit. Fast and low-cost techniques are important for infection screening in underdeveloped countries.
authors.affiliation: Surgical Pathology Service, Hospital das Clínicas, Universidade Federal do Triângulo Mineiro, 38025-180 Uberaba, Minas Gerais, Brazil.
authors.forename: Eliângela C
authors.initials: EC
authors.lastname: Côbo
authors.forename: Juliano C
authors.initials: JC
authors.lastname: Silva
authors.forename: Ulisses A
authors.initials: UA
authors.lastname: Cota
authors.forename: Juliana R
authors.initials: JR
authors.lastname: Machado
authors.forename: Lucio R
authors.initials: LR
authors.lastname: Castellano
mesh_terms: D003883:Arthrodermataceae / Q000166:cytology / Q000302:isolation & purification*; D002498:Centrifugation; D003881:Dermatomycoses / Q000175:diagnosis*; D005188:False Negative Reactions; D006801:Humans; D008853:Microscopy / Q000379:methods*; D009172:Mycology / Q000379:methods*; D012680:Sensitivity and Specificity