The tissue expression pattern of the AtGRP5 regulatory region is controlled by a combination of positive and negative elements.
The AtGRP5 gene from Arabidopsis thaliana encodes a glycine-rich protein which has a major activity in protoderm-derived cells and is expressed in cells that undergo the first anatomical modifications leading to somatic embryo development. It has been previously demonstrated that its minimum promoter is 316 bp long including the 5'UTR and presents three putative TATA-boxes sequences and several regions that are homologous to previous characterized cis-acting elements. In order to better characterize the AtGRP5 expression and to identify the promoter regions involved in its preferential epidermal expression, in situ hybridization and 5' promoter deletions were employed. In situ hybridization and GUS expression assays indicate that, besides being present during somatic embryogenesis, AtGRP5 is also expressed during the zygotic embryo development. The sequential 5' deletions indicate that multiple negative and positive regulatory elements are present in the AtGRP5 promoter and operate in order to confer its distinct expression pattern. A 44-bp region was shown to be essential for the epidermal expression of this gene in leaves, stems, flowers and fruits, and is also responsible for high activity of the AtGRP5 promoter in zygotic embryos. An element responsible for the phloem expression was also identified in a 35-bp region.
Mangeon
Amanda
A
Laboratório de Genômica Funcional e Transdução de Sinal, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Magioli
Claudia
C
Tarré
Erika
E
Cardeal
Vanessa
V
Araujo
Cristina
C
Falkenbach
Erica
E
Rocha
Carla Andréa Benício
CA
Rangel-Lima
Camila
C
Sachetto-Martins
Gilberto
G
D017360:Arabidopsis / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D016385:TATA Box; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins