title: Moderate hypothermia suppressed excessive generation of superoxide anion radical and inflammatory reactions in blood and liver in heatstroke: laboratory study in rats.
abstract: Abstract The study was performed to demonstrate superoxide radical (O(2).-) generation, systemic inflammation and liver injury caused by heatstroke and to reveal suppressive effects of moderate hypothermia. Heatstroke was defined as achieving pharyngeal temperature of 40 degrees C with arterial pressure reduction. Heatstroke rats were divided to four groups by the temperature after the onset; 40 degrees C, 37 degrees C, 32 degrees C and sham-treated with 37 degrees C. O(2).- current was measured continuously in the right atrium using an electrochemical O(2).- sensor. The O(2).- current increased in all groups except for the sham-treated group during the induction. After the onset of heatstroke, the O(2).- current was suppressed with temperature-dependency. Plasma and liver high-mobility group box 1, intercellular adhesion molecule-1, plasma aspartate aminotransferase and alanine aminotransferase were also suppressed with the suppression of O(2).- generation. Therefore, excessive O(2).- generation might be a key factor in heatstroke and the suppression with moderate hypothermia would be a therapeutic modality.
authors.affiliation: Advanced Medical Emergency and Critical Care Center, Yamaguchi University Hospital, Ube, Yamaguchi, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Todani
authors.forename: Motoki
authors.initials: M
authors.lastname: Fujita
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Tsuruta
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakahara
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yagi
authors.forename: Chiyomi
authors.initials: C
authors.lastname: Oshima
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Igarashi
authors.forename: Koshiro
authors.initials: K
authors.lastname: Takahashi
authors.forename: Shunji
authors.initials: S
authors.lastname: Kasaoka
authors.forename: Makoto
authors.initials: M
authors.lastname: Yuasa
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Maekawa
mesh_terms: D000140:Acidosis, Lactic / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001794:Blood Pressure; D004195:Disease Models, Animal; D024243:HMGB1 Protein / Q000378:metabolism; D018883:Heat Stroke / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D007036:Hypothermia, Induced; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D017736:Ion-Selective Electrodes; D008099:Liver / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000097:blood / Q000378:metabolism*; D013997:Time Factors
chemical_list: D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; D018836:Inflammation Mediators; D013481:Superoxides; D018799:Intercellular Adhesion Molecule-1; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase