[Involvement of endoplasmic reticulum stress in development of fatty liver fibrosis induced by methionine-choline-deficient diet in rats].
OBJECTIVE
To study role of endoplasmic reticulum stress in the development of fatty liver fibrosis induced by methionine-choline-deficient diet in rats.


METHODS
Non-alcoholic steatohepatitis was induced by 10 weeks- methionine-choline-deficient diet (MCDD), Markers of endoplasmic reticulum stress were determined by immunoblotting and real-time PCR.


RESULTS
The number of apoptotic hepatocytes, The expression levels of endoplasmic reticulum stress markers were increased significantly in MCDD group compared to control group (probability value less than 0.05 or probability value less than 0.01), while ratio of hepatocyte proliferation/apoptosis was decreased in MCDD group (probability value less than 0.01). The number of hepatocytes apoptosis, and the expression levels of endoplasmic reticulum stress markers were decreased significantly 2 weeks after the feeding with normal diet in MCDD group (probability value less than 0.05 or probability value less than 0.01).


CONCLUSIONS
MCDD induces endoplasmic reticulum stress and fibrosis in rats.
Mu
Yong-ping
YP
Shanghai Public Health Clinical Centre, Shanghai 201508, China. ypmu8888@126.com
Kwada
Norifumi
N
Ogawa
Tomohiro
T
Xi
Xiu-hong
XH
Chen
Xiao-rong
XR
D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D002794:Choline / Q000008:administration & dosage / Q000378:metabolism; D002796:Choline Deficiency; D004032:Diet; D004195:Disease Models, Animal; D004721:Endoplasmic Reticulum / Q000502:physiology*; D005234:Fatty Liver / Q000150:complications*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000178:diet therapy* / Q000209:etiology / Q000503:physiopathology*; D008297:Male; D008715:Methionine / Q000172:deficiency; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D012333:RNA, Messenger; D008715:Methionine; D020169:Caspases; D002794:Choline