Distribution of 14C-bisphenol A in pregnant and newborn mice.
OBJECTIVE
The purpose of the present investigation was to trace the fate of bisphenol A injected into pregnant mice, focusing on its potential accumulation in the fetus and the brain, critical targets of hormonal chemicals, using whole-body autoradiography.


METHODS
Pregnant mice were injected intraperitoneally with 0.46MBq of (14)C-BPA and then killed at 1h or 1, 3, or 5 days after injection. Sections for autoradiography were prepared in a cryomicrotome and the exposed imaging plate was processed using a fluorescent/radioisotope image analyzer.


RESULTS
Intraperitoneally injected (14)C-BPA was distributed throughout the body, including the fetus and the brain, within 1h. Radioactivity faded gradually from the whole body by the fifth day, and no accumulation in any specific organ was found. However, although (14)C was detected in the fetuses immediately after injection, the transfer of BPA from mother to newborn was not observed.


CONCLUSIONS
The routes of rapid BPA discharge were confirmed, and BPA neither accumulated in the body nor was it transferred to newborn mice. No evidence was observed to suggest the existence of a blood-placenta or blood-brain barrier for BPA. This information should be taken into consideration when assessing the risks of using dental materials that contain BPA.
Tanaka
Mitsuro
M
Department of Pediatric Dentistry, Iwate Medical University, 19-1 Uchimaru, Morioka, Japan. mtanaka@iwate-med.ac.jp
Kawamoto
Tadafumi
T
Matsumoto
Hiroki
H
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D001559:Benzhydryl Compounds; D001812:Blood-Brain Barrier / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D016616:Cryoultramicrotomy; D004968:Estrogens, Non-Steroidal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D005333:Fetus / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007274:Injections, Intraperitoneal; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000196:embryology / Q000378:metabolism; D008431:Maternal-Fetal Exchange; D051379:Mice; D010636:Phenols / Q000008:administration & dosage / Q000493:pharmacokinetics*; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy / Q000378:metabolism*; D019275:Radiopharmaceuticals; D013997:Time Factors; D014018:Tissue Distribution; D051598:Whole Body Imaging; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D002250:Carbon Radioisotopes; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D019275:Radiopharmaceuticals; C006780:bisphenol A; D000098826:Bisphenol A Compounds