Lack of effects of single nucleotide polymorphisms of the DNA methyltransferase 1 gene on gastric cancer in Iranian patients: a case control study.
BACKGROUND
Gastric cancer is one of the most common malignant tumors in Iran. Hypomethylation and/or hypermethylation of DNA has been described in gastric cancer and is presumed to be an early event in carcinogeneisis.


OBJECTIVE
We therefore hypothesized that single nucleotide polymorphisms of the DNMT1 gene may be associated with the genetic susceptibility to gastric cancer.


METHODS
Totals of 200 patients and 200 controls, both of Iranian origin, were studied. Three polymorphisms were genotyped by PCR-RFLP and allele frequencies and genotypes were compared between the cases and controls. Odds ratios were calculated and the interactions between the polymorphisms, age and sex were examined.


RESULTS
There were no significant associations between the DNMT1 polymorphisms and gastric cancer.


CONCLUSIONS
We could not show any association between DNMT1 polymorphisms and gastric cancer. Larger sets of polymorphisms and sample sizes are required for future testing of possible associations.
Khatami
Fatemeh
F
Research Center for Gastrointestinal and Liver Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran. sKhatami@rigld.ir
Noorinayer
Babak
B
Ghiasi
Somayeh
S
Mohebi
Reza
R
Hashemi
Mohhamad
M
Zali
Mohammad Reza
MR
D000328:Adult; D016022:Case-Control Studies; D019175:DNA Methylation; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012097:Repressor Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000235:genetics*
C411744:DMAP1 protein, human; D012097:Repressor Proteins