Expression of endothelin-1 and endothelin receptor a in canine ovarian tumours.
Ovarian tumours have a low incidence in bitch. Endothelin (ET-1) and endothelin A receptor (ET-A) are overexpressed in human ovarian cancer. Twenty canine ovarian tumours and five normal samples were first evaluated by western blotting and then immunohistochemically for ET-1 and ET-A expression. Seventeen out of twenty tumours were ET-1 positive. Eight out of twenty tumours were ET-A immunohistochemically positive. At molecular level both proteins were proven to be expressed in normal as well as in tumour samples. Our results show that ET-1 and ET-A are overexpressed in canine ovarian tumours, suggesting a potential role of these two molecules in canine ovarian carcinogenesis.
Borzacchiello
G
G
Department of Pathology and Animal Health, General Pathology and Anatomic Pathology Division, Naples, Italy. borzacch@unina.it
Mogavero
S
S
Tortorella
G
G
Catone
G
G
Russo
M
M
D000818:Animals; D002291:Carcinoma, Papillary / Q000378:metabolism / Q000662:veterinary; D003536:Cystadenocarcinoma / Q000378:metabolism / Q000662:veterinary; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D019332:Endothelin-1 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006106:Granulosa Cell Tumor / Q000378:metabolism / Q000662:veterinary; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000662:veterinary*; D044022:Receptor, Endothelin A / Q000235:genetics / Q000378:metabolism*
D019332:Endothelin-1; D044022:Receptor, Endothelin A