title: IFN-γ induces IFN-α and IFN-β expressions in cultured rat intestinal mucosa microvascular endothelial cells.
abstract: Although researchers have recently begun to pay more attention to the immunological characteristics of microvascular endothelial cells (MVECs), there are no reports on whether activation of MVECs by interferon-γ (IFN-γ) exerts any influence on the expressions of IFN-α/β. In the present study, we examined the influence of IFN-γ on the expressions of IFN-α/β in rat intestinal mucous MVECs (RIMMVECs). Different concentrations of IFN-γ were used to stimulate cultured RIMMVECs in vitro, and the cells and cell supernatants were collected at different time intervals. The influence of IFN-γ on the expressions of IFN-α/β in the RIMMVECs was examined at the mRNA and protein levels by real-time quantitative PCR and enzyme-linked immunosorbent assay (ELISA), respectively. The results indicated that IFN-γ was able to activate RIMMVECs, thereby leading to upregulated expressions of IFN-α/β. The real-time quantitative PCR analyses indicated that the IFN-α/β mRNA expression levels in RIMMVECs achieved their peak values after stimulation with IFN-γ at 20 ng/mL for 6 h and were increased by 14.88- and 3.82-fold, respectively, when compared with the levels in negative control cells. The ELISA analyses revealed that the IFN-α/β protein expression levels achieved their peak values after stimulation with IFN-γ at 40 ng/mL. The expression of IFN-α protein achieved its peak value at 12 h, while the expression of IFN-β protein achieved its peak value after 6 h. The present results suggest that the expression and secretion of IFNs may participate in the immunologic barrier function of MVECs.
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China.
authors.forename: Ge
authors.initials: G
authors.lastname: Hu
authors.forename: Jiuzhou
authors.initials: J
authors.lastname: Xue
authors.forename: Huiqin
authors.initials: H
authors.lastname: Duan
authors.forename: Zuojun
authors.initials: Z
authors.lastname: Yang
authors.forename: Liyun
authors.initials: L
authors.lastname: Gao
authors.forename: Haoshu
authors.initials: H
authors.lastname: Luo
authors.forename: Xiang
authors.initials: X
authors.lastname: Mu
authors.forename: Sheng
authors.initials: S
authors.lastname: Cui
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D005169:Factor VIII / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D016898:Interferon-alpha / Q000235:genetics / Q000378:metabolism*; D016899:Interferon-beta / Q000235:genetics / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000166:cytology*; D055806:Microvessels / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016898:Interferon-alpha; D016899:Interferon-beta; D007371:Interferon-gamma; D005169:Factor VIII