Replication of not-known-vector flaviviruses in mosquito cells is restricted by intracellular host factors rather than by the viral envelope proteins.
Chimeric yellow fever virus 17D (YFV-17D) and dengue virus type 2 (DENV2) carrying the surface proteins of Modoc virus (MODV), a not-known-vector (NKV) flavivirus, replicated efficiently in mammalian (Vero-B) and mosquito (C6/36) cells, whereas MODV failed to replicate in mosquito cells. Transfection of C6/36 cells with MODV RNA did not result in virus replication; however, transfection of these mosquito cells with YFV-17D or DENV2 RNA did. The inability of NKV viruses (such as MODV) to infect and replicate in arthropod cells is thus not determined by the viral envelope, but by a post-entry event.
Charlier
Nathalie
N
Laboratory of Virology and Chemotherapy, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
Davidson
Andrew
A
Dallmeier
Kai
K
Molenkamp
Richard
R
De Clercq
Erik
E
Neyts
Johan
J
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D009033:Culicidae / Q000166:cytology*; D005416:Flavivirus / Q000502:physiology*; D015967:Gene Expression Regulation, Viral; D054884:Host-Pathogen Interactions; D012367:RNA, Viral; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D053586:Virus Internalization; D014779:Virus Replication
D012367:RNA, Viral; D014759:Viral Envelope Proteins