title: Regulation of leukocyte recruitment by the long pentraxin PTX3.
abstract: Pentraxins are a superfamily of conserved proteins involved in the acute-phase response and innate immunity. Pentraxin 3 (PTX3), a prototypical member of the long pentraxin subfamily, is a key component of the humoral arm of innate immunity that is essential for resistance to certain pathogens. A regulatory role for pentraxins in inflammation has long been recognized, but the underlying mechanisms remain unclear. Here we report that PTX3 bound P-selectin and attenuated neutrophil recruitment at sites of inflammation. PTX3 released from activated leukocytes functioned locally to dampen neutrophil recruitment and regulate inflammation. Antibodies have glycosylation-dependent regulatory effect on inflammation. Therefore, PTX3, which is an essential component of humoral innate immunity, and immunoglobulins share functional outputs, including complement activation, opsonization and, as shown here, glycosylation-dependent regulation of inflammation.
authors.affiliation: Laboratory for Immunology and Inflammation, Instituto di Ricovero e Cura a Carattere Scientifico-Istituto Clinico Humanitas, Rozzano, Italy.
authors.forename: Livija
authors.initials: L
authors.lastname: Deban
authors.forename: Remo Castro
authors.initials: RC
authors.lastname: Russo
authors.forename: Marina
authors.initials: M
authors.lastname: Sironi
authors.forename: Federica
authors.initials: F
authors.lastname: Moalli
authors.forename: Margherita
authors.initials: M
authors.lastname: Scanziani
authors.forename: Vanessa
authors.initials: V
authors.lastname: Zambelli
authors.forename: Ivan
authors.initials: I
authors.lastname: Cuccovillo
authors.forename: Antonio
authors.initials: A
authors.lastname: Bastone
authors.forename: Marco
authors.initials: M
authors.lastname: Gobbi
authors.forename: Sonia
authors.initials: S
authors.lastname: Valentino
authors.forename: Andrea
authors.initials: A
authors.lastname: Doni
authors.forename: Cecilia
authors.initials: C
authors.lastname: Garlanda
authors.forename: Silvio
authors.initials: S
authors.lastname: Danese
authors.forename: Giovanni
authors.initials: G
authors.lastname: Salvatori
authors.forename: Marica
authors.initials: M
authors.lastname: Sassano
authors.forename: Virgilio
authors.initials: V
authors.lastname: Evangelista
authors.forename: Barbara
authors.initials: B
authors.lastname: Rossi
authors.forename: Elena
authors.initials: E
authors.lastname: Zenaro
authors.forename: Gabriela
authors.initials: G
authors.lastname: Constantin
authors.forename: Carlo
authors.initials: C
authors.lastname: Laudanna
authors.forename: Barbara
authors.initials: B
authors.lastname: Bottazzi
authors.forename: Alberto
authors.initials: A
authors.lastname: Mantovani
mesh_terms: D055371:Acute Lung Injury / Q000276:immunology; D000818:Animals; D002097:C-Reactive Protein / Q000276:immunology*; D016466:CHO Cells; D002469:Cell Separation; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology; D007249:Inflammation / Q000276:immunology*; D036904:Leukocyte Rolling / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020556:Neutrophil Infiltration / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D000683:Serum Amyloid P-Component / Q000276:immunology*; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D011994:Recombinant Proteins; D000683:Serum Amyloid P-Component; D000099153:Pentraxins