title: Effects of treadmill exercise on cyclooxygenase-2 in the hippocampus in type 2 diabetic rats: correlation with the neuroblasts.
abstract: Cyclooxygenase (COX) is a rate-limiting enzyme in synthesis of prostaglandins from arachidonic acid. In this study, we observed the effects of a physical exercise on COX-2 immunoreactivity in the hippocampus using immunohistochemistry in rats. In addition, we examined effects of administration of a COX-2 inhibitor, celecoxib, on neuroblast differentiation. At 6weeks of age, Zucker lean control (ZLC) and Zucker diabetic fatty (ZDF) rats were put on a treadmill with or without running for 1h/session/day for 5weeks. The running speed was gradually increased from 16 to 22m/min with 2m/min per 2weeks. In the ZLC and ZDF rats, COX-2 immunoreaction was detected in the granule cell layer of the dentate gyrus and in the stratum pyramidale of the CA2/3 region; COX-2 immunoreaction in the CA1 region was hardly detected. In the exercised-ZLC and ZDF rats, COX-2 immunoreactivity was significantly increased compared to that in the ZLC and ZDF rats, showing that COX-2 immunoreactivity in the exercised-ZDF rats was slightly low than that in the exercised-ZDF rats. In addition, weak COX-2 immunoreactivity was shown in the CA1 region by exercise. On the other hand, the repeated oral administration of celecoxib to 4-week-old ZDF rats significantly decreased the neuroblasts in the subgranular zone of the dentate gyrus. These results suggest that COX-2 may be associated with the increase of synaptic plasticity or contacts in the hippocampus.
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Sun Shin
authors.initials: SS
authors.lastname: Yi
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Ok Kyu
authors.initials: OK
authors.lastname: Park
authors.forename: Bingchun
authors.initials: B
authors.lastname: Yan
authors.forename: Il Yong
authors.initials: IY
authors.lastname: Kim
authors.forename: Yo Na
authors.initials: YN
authors.lastname: Kim
authors.forename: Wook
authors.initials: W
authors.lastname: Song
authors.forename: Seung Myung
authors.initials: SM
authors.lastname: Moon
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Je Kyung
authors.initials: JK
authors.lastname: Seong
authors.forename: Yeo Sung
authors.initials: YS
authors.lastname: Yoon
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000502:physiology; D051546:Cyclooxygenase 2 / Q000378:metabolism* / Q000502:physiology; D048909:Diabetes Complications / Q000201:enzymology* / Q000473:pathology / Q000628:therapy*; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology* / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D005080:Exercise Test; D006624:Hippocampus / Q000201:enzymology*; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000201:enzymology / Q000473:pathology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D011924:Rats, Zucker; D013234:Stem Cells / Q000166:cytology / Q000201:enzymology / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D051546:Cyclooxygenase 2