title: A duplex real-time PCR assay for detection of mutT4 and mutT2 mutations in Mycobacterium tuberculosis of Beijing genotype.
abstract: To determine the polymorphism of mutT genes of Mycobacterium tuberculosis of Beijing genotype, we developed a duplex real-time PCR assay based on hybridization probes for the Roche LightCycler instrument. The assay rapidly detects mutations at codons 48 and 58 of genes mutT4 and at mutT2, respectively.
authors.affiliation: Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Università di Pisa, I-56127 Pisa, Italy. rindi@biomed.unipi.it
authors.forename: Laura
authors.initials: L
authors.lastname: Rindi
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Lari
authors.forename: Carlo
authors.initials: C
authors.lastname: Garzelli
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D005838:Genotype; D006801:Humans; D020125:Mutation, Missense; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
chemical_list: D001426:Bacterial Proteins