title: Site-specific pKa determination of the carboxylate-binding subunit in artificial peptide receptors.
abstract: Site-specific pK(a) determination of the carboxylate-binding subunit in artificial peptide receptors is achieved by pH-dependent UV resonance Raman scattering with subsequent matrix factorization. This quantitative methodology allows to determine accurately the local acidity of a binding site based on the complete vibrational spectrum and, most importantly, without the need of selecting any particular peak.
authors.affiliation: University of Osnabrück, Department of Physics, Barbarastr. 7, 49069 Osnabrück, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Niebling
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Srivastava
authors.forename: Christoph
authors.initials: C
authors.lastname: Herrmann
authors.forename: Peter R
authors.initials: PR
authors.lastname: Wich
authors.forename: Carsten
authors.initials: C
authors.lastname: Schmuck
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schlücker
mesh_terms: D002264:Carboxylic Acids / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D018000:Receptors, Peptide / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002264:Carboxylic Acids; D018000:Receptors, Peptide