title: Brevibacillus expression system: host-vector system for efficient production of secretory proteins.
abstract: Brevibacillus expression system is an effective bacterial expression system for secretory proteins. The host bacterium, Brevibacillus choshinensis, a gram-positive bacterium, has strong capacity to secrete a large amount of proteins (approximately 30 g/L), which mostly consist of cell wall protein. A host-vector system that utilizes such high expression capacity has been constructed for the production of secretory proteins and tested for various heterologous proteins, including cytokines, enzymes, antigens, and adjuvants.
authors.affiliation: Bio Research Group, R&D Department, Higeta Shoyu Co. Ltd., 2-8 Chuo-Cho, Choshi-shi, Chiba 288-8680, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Mizukami
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hanagata
authors.forename: Akira
authors.initials: A
authors.lastname: Miyauchi
mesh_terms: D001951:Brevibacterium / Q000502:physiology*; D024861:Genetic Enhancement / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins