title: Detection of group B Streptococcus bacteria in LIM enrichment broth by peptide nucleic acid fluorescent in situ hybridization (PNA FISH) and rapid cycle PCR.
abstract: The sensitivity, specificity, and negative and positive predictive values for the detection of group B Streptococcus (GBS) in 206 LIM enrichment broths by the use of subculture, GBS peptide nucleic acid fluorescent in situ hybridization (PNA FISH), and GBS PCR were 96.9%, 100%, 98.6%, and 100%; 98.4%, 100%, 99.3%, and 100%; and 100%, 100%, 100%, and 100%, respectively.
authors.affiliation: Cleveland Clinic, 9500 Euclid Avenue/L11, Cleveland, OH 44195, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Wilson
authors.forename: G S
authors.initials: GS
authors.lastname: Hall
authors.forename: G W
authors.initials: GW
authors.lastname: Procop
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007231:Infant, Newborn; D020135:Peptide Nucleic Acids; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D011247:Pregnancy; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D003470:Culture Media; D020135:Peptide Nucleic Acids