Preparation and agglutination of immuno-nanolatex for malaria diagnosis.
This is the first systematic study on the adsorption of malaria antigen onto latex particles to elaborate immuno-latex for diagnostic purpose. Immuno-latex was prepared from adsorption of malaria antigen onto polystyrene (PS) or poly(styrene-co-acrylic acid) (St/AA) latex particles and was used for detection of antibody to P. Falciparum based on agglutination test. Adsorption behavior of the malaria antigen onto polymer particles as a function of incubation time, antigen concentration, pH and concentration of the medium was studied. The prepared immuno-latexes were characterized in terms of immuno-agglutination and specific bridging using patient's plasma with P. Falciparum infection. This evaluation was performed using photon correlation spectroscopy.
Polpanich
Duangporn
D
Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Tangboriboonrat
Pramuan
P
Elaissari
Abdelhamid
A
D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood; D004339:Drug Compounding / Q000379:methods; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D008288:Malaria / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D016778:Malaria, Falciparum / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D008968:Molecular Conformation; D050997:Nanomedicine / Q000379:methods; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010963:Plasmodium falciparum / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000953:Antigens, Protozoan