title: Approaching marine bioprospecting in hexacorals by RNA deep sequencing.
abstract: RNA deep sequencing represents a new complementary approach in marine bioprospecting. Next-generation sequencing platforms have recently been developed for de novo whole transcriptome analysis, small RNA discovery and gene expression profiling. Deep sequencing transcriptomics (sequencing the complete set of cellular transcripts at a specific stage or condition) leads to sequential identification of all expressed genes in a sample. When combined to high-throughput bioinformatics and protein synthesis, RNA deep sequencing represents a new powerful approach in gene product discovery and bioprospecting. Here we summarize recent progress in the analyses of hexacoral transcriptomes with the focus on cold-water sea anemones and related organisms.
authors.affiliation: RNA and Transcriptomics Group, Department of Medical Biology, Faculty of Heath Sciences, University of Tromsø, Norway. Steinar.Johansen@uit.no
authors.forename: Steinar D
authors.initials: SD
authors.lastname: Johansen
authors.forename: Ase
authors.initials: A
authors.lastname: Emblem
authors.forename: Bård Ove
authors.initials: BO
authors.lastname: Karlsen
authors.forename: Siri
authors.initials: S
authors.lastname: Okkenhaug
authors.forename: Hilde
authors.initials: H
authors.lastname: Hansen
authors.forename: Truls
authors.initials: T
authors.lastname: Moum
authors.forename: Dag H
authors.initials: DH
authors.lastname: Coucheron
authors.forename: Ole Morten
authors.initials: OM
authors.lastname: Seternes
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003080:Cold Temperature; D019295:Computational Biology / Q000379:methods*; D020869:Gene Expression Profiling; D057166:High-Throughput Screening Assays; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009792:Oceans and Seas; D012313:RNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012615:Sea Anemones / Q000166:cytology / Q000235:genetics*; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA
chemical_list: D012313:RNA