Validation of an immunoturbidimetric method for determination of porcine serum C-reactive protein.
Measurement of porcine C-reactive protein (CRP) in serum is an important tool for monitoring health and welfare in pigs. In this study, an immunoturbidimetric method from Olympus System Reagent (OSR 6147) used to measure human CRP in serum that employ a human traceable calibrator has been evaluated in porcine serum samples. Intra- and inter-assay imprecision were lower than that obtained with the porcine-specific commercially available ELISA. The expected difference in serum CRP between healthy and non-healthy pigs was detected. CRP values measured by the immunoturbidimetric method showed a good correlation with those obtained by ELISA, although differences in absolute CRP values were observed. When an in-house porcine standard was used a better agreement was obtained. In conclusion, the immunoturbidimetric method of Olympus can be used with porcine samples. The easier use of this method should facilitate the implementation of CRP serum determination for diagnostic and prognostic purposes in swine medicine. The results emphasize the need to establish species-specific standard and methods to decrease inter-laboratory discrepancies.
Saco
Yolanda
Y
Servei de Bioquímica Clínica Veterinària, Departament de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain.
Fraile
Lorenzo
L
Giménez
Mercè
M
Canalias
Francesca
F
Bassols
Anna
A
D000818:Animals; D002097:C-Reactive Protein / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007118:Immunoassay / Q000379:methods / Q000662:veterinary*; D009391:Nephelometry and Turbidimetry / Q000379:methods / Q000662:veterinary*; D015203:Reproducibility of Results; D013552:Swine; D013553:Swine Diseases / Q000097:blood* / Q000175:diagnosis
D002097:C-Reactive Protein