title: Plasmid profiling of Flavobacterium psychrophilum isolates from ayu (Plecoglossus altivelis altivelis) and other fish species in Japan.
abstract: In order to evaluate the genetic variability of the causative agent of cold water disease (CWD), plasmid profiling was used to characterize Flavobacterium (F.) psychrophilum isolates (n = 169). Size analysis of plasmids in F. psychrophilum isolates (n = 128) from several fish species demonstrated that six kinds of plasmids were harbored, and ayu isolates had different profiles compared to other isolates. Moreover, multiple isolates (n = 41) from CWD outbreaks in 2002 to 2003 at a single ayu farm were examined to determine differences between isolates from successive outbreaks and showed different profiles by the sources of seedlings.
authors.affiliation: Laboratory of Aquatic Animal Medicine & Brain Korea 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Ji Hyung
authors.initials: JH
authors.lastname: Kim
authors.forename: Dennis K
authors.initials: DK
authors.lastname: Gomez
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nakai
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Park
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks / Q000662:veterinary*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005393:Fish Diseases / Q000235:genetics / Q000382:microbiology*; D045826:Flavobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary*; D005417:Flavobacterium / Q000235:genetics / Q000302:isolation & purification*; D014644:Genetic Variation / Q000235:genetics*; D007564:Japan; D036441:Osmeriformes; D010957:Plasmids / Q000235:genetics
chemical_list: D004269:DNA, Bacterial