Peculiarities of Raman scattering in bioconjugated CdSe/ZnS quantum dots.
The article presents the results of analysis of Raman scattering spectra of non-conjugated and bioconjugated CdSe/ZnS core-shell quantum dots (QDs). Commercial CdSe/ZnS QDs used covered by polymer are characterized by color emission with the maxima at 605-610 nm (2.03-2.05 eV). The bioconjugation process is performed to biomolecules-the antihuman Interleukin 10 (IL10) antibodies (mab). Raman scattering spectra measured at room temperature with excitation by a He-Ne laser line (632.8 nm) demonstrate two groups of peaks: (1) related to the Si substrate at 230-460, 522, 610, 670, 940-1040 cm(-1) and (2) to the PEG polymer on the QD surface in the range of 837-3320 cm(-1). It is revealed that the CdSe/ZnS QD bioconjugation to the antihuman Interleukin 10 antibodies is accompanied with the dramatic changes in the intensity of the Raman lines of both types: the intensity of the Si related line increases six- or ten-fold, but the intensity of the polymer related line decreases ten-fold. The models explaining the mentioned effects in Raman scattering spectra have been discussed.
Cano
A Diaz
AD
CINVESTAV-IPN, Santiago de Querétaro, Querétaro, Mexico.
Sandoval
S Jiménez
SJ
Vorobiev
Y
Y
Melgarejo
F Rodriguez
FR
Torchynska
T V
TV
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D019187:Cadmium Compounds / Q000378:metabolism*; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology; D011092:Polyethylene Glycols / Q000737:chemistry; D045663:Quantum Dots; D018036:Selenium Compounds / Q000378:metabolism*; D012825:Silicon / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013440:Sulfides / Q000378:metabolism*; D017967:Zinc Compounds / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D016753:Interleukin-10; D011092:Polyethylene Glycols; C058667:cadmium selenide; C031238:zinc sulfide; D012825:Silicon