Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14.
The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl)furfural (HMF) and furfural are specifically notorious. In this study, we describe the complete molecular identification and characterization of the pathway by which Cupriavidus basilensis HMF14 metabolizes HMF and furfural. The identification of this pathway enabled the construction of an HMF and furfural-metabolizing Pseudomonas putida. The genetic information obtained furthermore enabled us to predict the HMF and furfural degrading capabilities of sequenced bacterial species that had not previously been connected to furanic aldehyde metabolism. These results pave the way for in situ detoxification of lignocellulosic hydrolysates, which is a major step toward improved efficiency of utilization of lignocellulosic feedstock.
Koopman
Frank
F
Department of Biotechnology, Bio-Based Sustainable Industrial Chemistry (B-Basic), Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands. f.w.koopman@tudelft.nl
Wierckx
Nick
N
de Winde
Johannes H
JH
Ruijssenaars
Harald J
HJ
D049921:Cupriavidus / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004251:DNA Transposable Elements / Q000235:genetics; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics; D053858:Metabolic Networks and Pathways; D016296:Mutagenesis / Q000235:genetics; D010088:Oxidoreductases / Q000378:metabolism; D010641:Phenotype; D016958:Pseudomonas putida / Q000378:metabolism
D004251:DNA Transposable Elements; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde; D010088:Oxidoreductases