Integration of SPR biosensors with mass spectrometry (SPR-MS).
The combination of surface plasmon resonance (SPR) and mass spectrometry (MS) creates a comprehensive protein investigation approach wherein SPR is employed for protein quantification and MS is utilized to structurally characterize the proteins. In such, MS utterly complements the SPR detection and reveals intrinsic protein structural modifications that go unregistered via the SPR detection. Protein complexes and non-specific binding can also be delineated via the SPR-MS approach. Described here are the protocols and know-how for successful and reproducible integration of SPR and MS. The individual steps of the entire SPR-MS process are illustrated via an example showing analysis of myoglobin from human plasma.
Nedelkov
Dobrin
D
Intrinsic Bioprobes Inc., Tempe, AZ, USA.
D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009211:Myoglobin / Q000097:blood / Q000737:chemistry / Q000276:immunology; D020349:Surface Plasmon Resonance / Q000379:methods*; D018511:Systems Integration
D009211:Myoglobin