The optimal extraction parameters and anti-diabetic activity of flavonoids from Ipomoea batatas leaf.
Extraction parameters of flavonoids from Ipomoea batatas leaf (FIBL) and anti-diabetic activity of FIBL on alloxan induced diabetic mice were studied. The optimal extraction parameters of FIBL were obtained by single factor test and orthogonal test, as follows: ethanol concentration 60 %, ratio of solvent to raw material 30, extraction temperature 75 degrees and extraction time 1.5 h, while extraction yield of FIBL was 5.94 %. FIBL treatment (50, 100, and 150 mg/ kg body weight) for 28 days resulted in a significant decrease in the concentration of fasting blood glucose (FBG), total cholesterol (TC) and triglyceride (TG) in diabetes mellitus mice. Furthermore, FIBL significantly increased body weight (bw) and serum high-density lipoprotein cholesterol (HDL-c) level. The data demonstrated FIBL at the dose of 100 mg/kg bw exhibited the optimal effect. The above results suggest that FIBL can control blood glucose and modulate the metabolism of blood lipid in diabetes mellitus mice.
Li
Fenglin
F
Department of Biological Engineering, Yanshan University, Qinhuangdao 066004, P.R. China. swgclifenglin@sina.com
Li
Qingwang
Q
Gao
Dawei
D
Peng
Yong
Y
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D027723:Ipomoea batatas / Q000737:chemistry*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D008076:Cholesterol, HDL; D005419:Flavonoids; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D014280:Triglycerides; D002784:Cholesterol