Labdane diterpenes from the aerial parts of Curcuma comosa enhance fetal hemoglobin production in an erythroid cell line.
Three new labdane diterpenes, curcucomosins A-C (1-3), four known labdane diterpenes, 4-7, and a known diarylheptanoid, 8, were isolated from the aerial parts of Curcuma comosa. The structures of the new diterpenes were elucidated by spectroscopic data analysis. The fetal hemoglobin (Hb F) induction potency of the isolated compounds was examined using a K562 reporter cell line harboring the enhanced green fluorescence protein (EGFP) gene under the control of a (G)gamma-globin promoter. Compound 6, isocoronarin D, exhibited the highest Hb F induction effect of 1.6-fold at 20 microM.
Chokchaisiri
Ratchanaporn
R
Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand.
Chaneiam
Nattawara
N
Svasti
Saovaros
S
Fucharoen
Suthat
S
Vadolas
Jim
J
Suksamrarn
Apichart
A
D030024:Curcuma / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D041905:Erythroid Cells / Q000187:drug effects; D005319:Fetal Hemoglobin / Q000187:drug effects* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D020014:K562 Cells; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013785:Thailand
D004224:Diterpenes; C549722:curcucomosin A; C549723:curcucomosin B; C549724:curcucomosin C; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D005319:Fetal Hemoglobin