title: Human bone marrow mesenchymal stem cells expressing SDF-1 promote hematopoietic stem cell function of human mobilised peripheral blood CD34+ cells in vivo and in vitro.
abstract: OBJECTIVE
  There is mounting evidence demonstrating that stromal cell derived factor-1 (SDF-1) plays an important role in homing of hematopoietic progenitor cells to bone marrow. This study was aimed to assess whether bone marrow mesenchymal stem cells overexpressing exogenous SDF-1 could synergistically promote the homing of CD34(+) (Cluster of Differentiation [CD]) cells to bone marrow of lethally irradiated severe combined immunodeficiency (SCID) mice.
  
  
  METHODS
  Human SDF-1 complementary Deoxyribonucleic acid (cDNA) was transfected into bone marrow-derived mesenchymal stem cells with recombinant lentiviral vector. The expression of SDF-1 was detected by real-time Polymerase Chain Reaction (PCR) and Enzyme-Linked Immunosorbent Assay (ELISA), and the ex vivo chemotaxis function on CD34(+) cells was measured by coculture system and Transwell system. SDF-1 gene-modified mesenchymal stem cell (MSC) and CD34(+) cells were infused into lethally irradiated SCID mice and the hematopoietic reconstitution in the recipient mice was examined.
  
  
  RESULTS
  Messenger ribonucleic acid (mRNA) and protein of SDF-1 in infected MSC were significantly higher than that of the non-infected control MSC (p < 0.05). The infected MSC have significant chemotaxis effect on CD34(+) cells in vitro and promote hematopoietic reconstitution after CD34(+) cell transplantation in vivo.
  
  
  CONCLUSIONS
  MSC with high-level expression of SDF-1 can synergistically promote hematopoietic reconstitution after CD34(+) cell transplantation in lethally irradiated SCID mice.
authors.affiliation: The Third Military Medical University, Department of Hematology, Xinqiao Hospital, Chongqing, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Liang
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Su
authors.forename: Pei-Yan
authors.initials: PY
authors.lastname: Kong
authors.forename: Dong-Feng
authors.initials: DF
authors.lastname: Zeng
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Chen
authors.forename: Xian-Gui
authors.initials: XG
authors.lastname: Peng
authors.forename: Zhong-Min
authors.initials: ZM
authors.lastname: Zou
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002465:Cell Movement; D017690:Cell Transplantation; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D002633:Chemotaxis / Q000235:genetics / Q000502:physiology; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D016513:Mice, SCID; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011833:Radiation Injuries, Experimental; D014162:Transfection
chemical_list: D018952:Antigens, CD34; D054377:Chemokine CXCL12; D012333:RNA, Messenger