[Investigation on arboviruses at Sino-Vietnam border areas in Wenshan of Yunnan province].
OBJECTIVE
To investigate arboviruses in Wenshan and Hekou county which are the Sino-Vietnam frontier regions of Wenshan, Yunnan province, China.


METHODS
In September 2007, 6091 Culicines, 1334 Anophelines, 848 Aedes vexans and 53 Armigeres obturbans were collected from 5 field sites. Mosquitoes were collected and stored in liquid nitrogen after classification. The mosquito pools were homogenized, and centrifuged, then the supematant was inoculated onto C6/36 and BHK-21 cells, and the viral isolates were identified by serological and molecular biological methods.Sequence alignment and phylogenetic analysis on the viral isolates were carried out using Clustal X 1.85, GENEDOC and MEGA4 software.


RESULTS
A total of 4 pairs of virus isolated with C6/36 cells cytopathic effect were observed, and other mosquito species have not cytopathic effect.Strain WS0704-2 was Banna virus which identified by antibody response and PCR. Strain WS0704-1, WS0708-1, WS0708-2 were Culex pipens pallens densovirus (CppDNV) which identified by PCR. The phylogenetic analysis the 12th segment showed significant difference between the new Banna virus and other strains isolated in China.


CONCLUSIONS
There are many mosquito vectors in frontier regions (China and Vietnam) of Wenshan in Yunnan province of China, and mosquito-borne arbovirus, such as BAV were isolated here.
Zhou
Tao
T
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Zhang
Hai-lin
HL
Li
Ming-hua
MH
Wang
Jing-lin
JL
Fu
Shi-hong
SH
Feng
Yun
Y
Liang
Guo-dong
GD
D000818:Animals; D001103:Arboviruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D009033:Culicidae / Q000821:virology*; D017990:Densovirus / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA