Spectrometric study of the interaction between alpinetin and bovine serum albumin using chemometrics approaches.
The binding interaction of Alpinetin (APT) with bovine serum albumin (BSA) was studied by fluorescence, UV-visible and synchronous fluorescence spectroscopy (SFS) under simulated physiological conditions. The measured complex spectra were resolved by multivariate curve resolution-alternating least squares (MCR-ALS), yielding a host of data and information, which otherwise would have been impossible to obtain. The extracted profiles corresponded to the spectra of the single species in the APT/BSA mixture. In addition, the presence of the APT-BSA complex was demonstrated, and it was shown that the associated quenching of the fluorescence from the BSA protein resulted from the formation of APT-BSA complex via a static mechanism. The binding constant (K(a(ave))=2.34 x 10(6) L mol(-1)) and the number of sites (n=1) were obtained by fluorescence methods as were the thermodynamic parameters (DeltaH(0), DeltaS(0) and DeltaG(0)). This work suggested that the principal binding between APT to BSA was facilitated by hydrophobic interactions. The thermodynamic parameters for APT were compared to those from the structurally similar Chrysin and Wogonin molecules. It appeared that the entropy parameters were relatively more affected by the small structural changes. SFS from the interaction of BSA and APT showed that the ligand affected the conformation of BSA. The competitive interaction of APT and site makers with BSA indicated site I as the binding area of APT in BSA.
Ni
Yongnian
Y
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. ynni@ncu.edu.cn
Wang
Shuangshuang
S
Kokot
Serge
S
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D044950:Flavanones / Q000737:chemistry*; D005453:Fluorescence; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D044950:Flavanones; C436748:alpinetin; D012710:Serum Albumin, Bovine