title: Dipeptidyl-peptidase 4 and attractin expression is increased in circulating blood monocytes of obese human subjects.
abstract: Dipeptidyl-peptidase (DPP)-4, which catalizes the degradation of the insulinotropic incretin glucagon-like-peptide (GLP)-1, and the DPP-4 like enzyme attractin are involved in activation of T-lymphocytes and monocytes. Recently, it has been demonstrated, that the risk for certain infections is increased in type 2 diabetic patients under DPP-4 inhibitor treatment. The aim of the present study was to examine the expression of DPP-4 and attractin in circulating blood monocytes of obese and type 2 diabetic subjects. Monocytes were isolated by CD14-antibody based magnetic cell sorting from blood samples of 17 lean controls, 20 obese, non-diabetic subjects and 19 obese patients with type 2 diabetes. FACS analysis was performed to test purity of the cell preparations. Expression was measured by multiplex RT-PCR on RNA-level. DPP-4 and attractin were detectable in human circulating monocytes with attractin being expressed at higher levels compared to DPP-4. Both enzymes were significantly higher expressed in circulating blood monocytes of obese subjects compared to lean controls. In contrast, type 2 diabetes did not significantly affect expression levels. Finally, neither DPP-4 nor attractin expression was altered by sitagliptin or insulin treatment. In conclusion, our data demonstrate, that expressions of DPP-4 and attractin in circulating blood monocytes of human subjects are influenced by metabolic abnormalities with obesity being an important factor.
authors.affiliation: Department of Internal Medicine II, University of Cologne, Germany. matthias.laudes@uk-koeln.de
authors.forename: M
authors.initials: M
authors.lastname: Laudes
authors.forename: F
authors.initials: F
authors.lastname: Oberhauser
authors.forename: D M
authors.initials: DM
authors.lastname: Schulte
authors.forename: K
authors.initials: K
authors.lastname: Schilbach
authors.forename: S
authors.initials: S
authors.lastname: Freude
authors.forename: R
authors.initials: R
authors.lastname: Bilkovski
authors.forename: O
authors.initials: O
authors.lastname: Schulz
authors.forename: M
authors.initials: M
authors.lastname: Faust
authors.forename: W
authors.initials: W
authors.lastname: Krone
mesh_terms: D015992:Body Mass Index; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000188:drug therapy / Q000201:enzymology; D018819:Dipeptidyl Peptidase 4 / Q000097:blood*; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000627:therapeutic use; D005260:Female; D052216:Glucagon-Like Peptide 1 / Q000097:blood / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use; D007328:Insulin / Q000627:therapeutic use; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology*; D009765:Obesity / Q000097:blood* / Q000201:enzymology; D011719:Pyrazines / Q000627:therapeutic use; D000068900:Sitagliptin Phosphate; D014230:Triazoles / Q000627:therapeutic use
chemical_list: C492192:ATRN protein, human; D054873:Dipeptidyl-Peptidase IV Inhibitors; D007004:Hypoglycemic Agents; D007328:Insulin; D018950:Lipopolysaccharide Receptors; D008565:Membrane Proteins; D011719:Pyrazines; D014230:Triazoles; D052216:Glucagon-Like Peptide 1; D018819:Dipeptidyl Peptidase 4; D000068900:Sitagliptin Phosphate