Effect of administration route on the biodistribution and shedding of replication-deficient AAV2: a qualitative modelling approach.
Gene therapy is a rapidly developing field in which recombinant nucleic acid sequences are introduced to individuals to regulate, repair, replace, add or delete a genetic sequence. Recombinant adeno-associated viral (AAV) vectors, especially AAV2, are frequently used in gene therapy. Knowledge on the biodistribution and potential shedding of AAV2 is crucial to evaluate the risks of infection with the viral vector for the patient and the environment. Literature was analysed for biodistribution and shedding data for AAV2. Preclinical and clinical studies were included with a focus on the influence of the administration route on spreading. Based on biodistribution and shedding data, a qualitative model for the biodistribution and shedding of AAV2 related to the administration route is presented. It is concluded that biodistribution and shedding of AAV2 depends on the route of administration. Some routes lead to local biodistribution and thus to no shedding or shedding via one route only. Other routes lead to systemic biodistribution and to shedding via several excretion routes. The qualitative model presented can help to determine the possible biodistribution in the body and the risk of shedding via the different excretion routes. In addition, it can help to predict the different shedding routes after a certain administration route of AAV2 and thus in deciding which studies are warranted or which safety precautions are needed after administration to patients.
Brandon
Esther F A
EF
Centre for Substances and Integrated Risk Assessment, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands. esther.brandon@rivm.nl
Hermsen
Harm P H
HP
van Eijkeren
Jan C H
JC
Tiesjema
Birgitte
B
D000818:Animals; D002986:Clinical Trials as Topic; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000493:pharmacokinetics; D000229:Dependovirus / Q000235:genetics*; D004333:Drug Administration Routes; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage / Q000493:pharmacokinetics; D006801:Humans; D008954:Models, Biological; D014779:Virus Replication; D017201:Virus Shedding
D004274:DNA, Recombinant