title: A simple, sensitive determination of ganciclovir in infant plasma by high-performance liquid chromatography with fluorescence detection.
abstract: This study developed a simple and sensitive method using reversed-phase high-performance liquid chromatography (HPLC) for ganciclovir (GCV) plasma concentrations in cytomegalovirus infectious infants with hearing loss. The method involves a simple protein precipitation procedure that uses no solid-phase or liquid-liquid extraction. The HPLC separation was carried out on a Cadenza CD-C(18) column (3 microm, 4.6 mm x 150 mm) with phosphate buffer (pH 2.5, 25 mM) containing 1% methanol-acetonitrile mixture (4:3, v/v) as a mobile phase at a 0.7 mL/min flow rate. GCV was detected using a fluorescence detection (lambdaex/em: 265/380 nm). The quantification limit was 0.025 microg/mL for 100 microL of plasma sample at which good intra- and inter-assay coefficient of variation values (< 4.96%) and recoveries (94.9-96.5%) were established.
authors.affiliation: Saitama Children's Medical Center, Division of Clinical Research and Department of Pharmacy, Japan. a0183776@pref.saitama.lg.jp
authors.forename: Terumitsu
authors.initials: T
authors.lastname: Yoshida
authors.forename: Ryohei
authors.initials: R
authors.lastname: Takahashi
authors.forename: Koichi
authors.initials: K
authors.lastname: Imai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uchida
authors.forename: Yasutoshi
authors.initials: Y
authors.lastname: Arai
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Oh-ishi
mesh_terms: D000998:Antiviral Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000191:economics / Q000379:methods*; D005453:Fluorescence; D015774:Ganciclovir / Q000097:blood*; D006801:Humans; D007223:Infant; D057230:Limit of Detection; D016014:Linear Models
chemical_list: D000998:Antiviral Agents; D015774:Ganciclovir