Novosphingobium soli sp. nov., isolated from soil.
A yellow-pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium, strain CC-TPE-1(T), was isolated from oil-contaminated soil near an oil refinery located in Kaohsiung County, Taiwan. 16S rRNA gene sequence analysis of strain CC-TPE-1(T) showed highest sequence similarity to Novosphingobium naphthalenivorans TUT562(T) (98.1 %), N. panipatense SM16(T) (97.9 %) and N. mathurense SM117(T) (97.6 %) and lower (<97 %) sequence similarity to all other Novosphingobium species. DNA-DNA hybridizations of strain CC-TPE-1(T) with N. naphthalenivorans DSM 18518(T), N. panipatense SM16(T) and N. mathurense SM117(T) showed low relatedness of 30 % (reciprocal 35 %), 29.1 % (reciprocal 30.6 %) and 35 % (reciprocal 23.6 %), respectively. The major respiratory quinone was ubiquinone Q-10, the predominant fatty acid was C(18 : 1)ω7c (49.9 %) and three 2-hydroxy fatty acids, C(14 : 0) 2-OH (8.2 %), C(15 : 0) 2-OH (2.45 %) and C(16 : 0) 2-OH (1.05 %), were detected. Polar lipids consisted mainly of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidyldimethylethanolamine, two sphingoglycolipids, phosphatidylmonomethylethanolamine and several unidentified lipids, and a yellow pigment was also detected. The polyamine pattern contained the single major compound spermidine. Characterization by 16S rRNA gene sequence analysis, physiological parameters, pigment analysis and polyamine, ubiquinone, polar lipid and fatty acid compositions revealed that strain CC-TPE-1(T) represents a novel species of the genus Novosphingobium, for which we propose the name Novosphingobium soli sp. nov., with the type strain CC-TPE-1(T) (=DSM 22821(T) =CCM 7706(T) =CCUG 58493(T)).
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Young
Chiu-Chung
CC
Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Busse
Hans-Jürgen
HJ
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Lin
Shi-Yao
SY
Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Rekha
P D
PD
Yenepoya Research Center, Yenepoya University, University Rd, Deralakatee, Mangalore, Karnataka State, India.
Arun
A B
AB
Yenepoya Research Center, Yenepoya University, University Rd, Deralakatee, Mangalore, Karnataka State, India.
Chen
Wen-Ming
WM
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC.
Shen
Fo-Ting
FT
Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Wu
Y-H
YH
Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D010860:Pigments, Biological / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013624:Taiwan; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D008055:Lipids; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10