title: Purification and structure determination of gelatinase and collagenase inhibitors from Viola patrinii fermentation extracts.
abstract: Investigation of collagenase and gelatinase inhibitory natural components afforded two isoflavonoids. Two isoflavonoids, tectorigenin-7-O-β-D-glucoside (1) and luteolin-7-O-β-D-glucuronopyranoside (2), were isolated from ethyl acetate fraction of Viola patrinii fermentation extracts (VPFE). Of these, compounds 1 and 2 exhibited collagenase inhibitory activity (IC(50)) at a concentration of less than 1.5 μM, and compound 2 showed gelatinases A and B inhibitory activity (IC(50)) at 0.3 μM and 0.8 μM, respectively.
authors.affiliation: College of Natural Resources and Life Science, BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714, South Korea.
authors.forename: Kyoung-Sook
authors.initials: KS
authors.lastname: Kim
authors.forename: Yeon-Joo
authors.initials: YJ
authors.lastname: Kwak
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Kim
authors.forename: Hai Yang
authors.initials: HY
authors.lastname: Yu
authors.forename: Byoung-Won
authors.initials: BW
authors.lastname: Kang
authors.forename: Eunsook
authors.initials: E
authors.lastname: Chung
authors.forename: Young-Choon
authors.initials: YC
authors.lastname: Lee
authors.forename: Jung-In
authors.initials: JI
authors.lastname: Kim
authors.forename: Jai-Heon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D055162:Biocatalysis / Q000187:drug effects; D002845:Chromatography / Q000379:methods; D017364:Collagenases / Q000378:metabolism; D005285:Fermentation; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018093:Gelatinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D020128:Inhibitory Concentration 50; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D047311:Luteolin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D011480:Protease Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D032413:Viola / Q000378:metabolism / Q000382:microbiology*
chemical_list: D005419:Flavonoids; D007529:Isoflavones; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D011480:Protease Inhibitors; C456096:luteolin-7-O-glucuronide; C120040:tectoridin; D017364:Collagenases; D018093:Gelatinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D047311:Luteolin