[Extraction and separation of flavonoids from leave of Eucommia ulmoides in aqueous two-phase system].
OBJECTIVE
To optimize the aqueous two-phase extraction and separation technology of flavonoids from the leaves of Eucommia ulmoides.


METHODS
The system was prepared by dissolving suitable amounis of polyethylene glycol 4000 (PEG4000) and dextran 40000 ( D40) in aqueous solution. Phase map of the system, the mass fraction of PEG4000 and D40, the effects of amount of added sample, pH value and temperature on target compounds were investigated systematically.


RESULTS
The optimum extraction conditions were PEG4000 11%, N40 8%, 8 g sample which contains 6.85% of flavonoids, temperature 60 t and pH value 8. The highest extraction rate could reach to 75.82%.


CONCLUSIONS
This method has the advantages of mild conditions, solvent-free residues and less time-consumed, and the obvious efficiency in single-stage separation and purification of flavonoids.
Peng
Sheng
S
Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China. pengsheng162@163.com
Peng
Mi-jun
MJ
Huang
Mei-e
ME
Bu
Xiao-ying
XY
Wu
Gang
G
D005591:Chemical Fractionation / Q000379:methods*; D003911:Dextrans / Q000737:chemistry; D031283:Eucommiaceae / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
D003911:Dextrans; D005419:Flavonoids; D014867:Water; D011092:Polyethylene Glycols