title: Interactions of human bone cells with diamond-like carbon polymer hybrid coatings.
abstract: Diamond-like carbon (DLC) coatings produced using the plasma-accelerating filtered pulsed arc discharge (FPAD) method display excellent adherence to the substrate and improve its corrosion resistance. This article reports the interactions of human osteoblastic cells with DLC and two DLC polymer hybrid (DLC-p-h) coatings deposited on smooth, matt and rough silicon wafers by the FPAD method. The DLC-p-h materials were DLC-polytetrafluoroethylene hybrid (DLC-PTFE-h) and DLC-polydimethylsiloxane hybrid (DLC-PDMS-h) coatings. The biocompatibility of the coatings was assayed by using mesenchymal stem cells, primary osteoblasts and Saos-2 cells. Human mesenchymal stem cells proliferated when cultured on DLC and DLC-PTFE-h, but their numbers diminished on DLC-PDMS-h. In all three cell types studied, phalloidin-TRITC staining disclosed cell-type organization typical of an actin cytoskeleton on DLC and DLC-PTFE-h, but minimal and disorganized stress fibers on cells cultured on DLC-PDMS-h. The microtubular cytoskeleton was similarly disorganized on DLC-PDMS-h. Cells on DLC-PDMS-h developed a peculiar form of membrane damage, with nuclear staining by propidium iodide associated with granular calcein staining of the cytoplasm. Active caspase-3 labeling was only seen in cells cultured on DLC-PDMS-h, indicating that these cells undergo apoptosis induced by defective cell adhesion. Results suggest that DLC-PDMS-h coatings might be useful in orthopedic applications where an implant or implant-facet should be protected against bone overgrowth while DLC and DLC-PTFE-h coatings might improve osseointegration.
authors.affiliation: Unidad de Investigación, Hospital Universitario La Paz-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Calzado-Martín
authors.forename: Laura
authors.initials: L
authors.lastname: Saldaña
authors.forename: Hannu
authors.initials: H
authors.lastname: Korhonen
authors.forename: Antti
authors.initials: A
authors.lastname: Soininen
authors.forename: Teemu J
authors.initials: TJ
authors.lastname: Kinnari
authors.forename: Enrique
authors.initials: E
authors.lastname: Gómez-Barrena
authors.forename: Veli-Matti
authors.initials: VM
authors.lastname: Tiainen
authors.forename: Reijo
authors.initials: R
authors.lastname: Lappalainen
authors.forename: Luis
authors.initials: L
authors.lastname: Munuera
authors.forename: Yrjö T
authors.initials: YT
authors.lastname: Konttinen
authors.forename: Nuria
authors.initials: N
authors.lastname: Vilaboa
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000199:Actins / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology*; D053148:Caspase 3 / Q000378:metabolism; D002450:Cell Communication / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D018130:Diamond / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D011108:Polymers / Q000494:pharmacology*; D014404:Tubulin / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D000199:Actins; D020099:Coated Materials, Biocompatible; D011108:Polymers; D014404:Tubulin; D014867:Water; D018130:Diamond; D053148:Caspase 3