title: Precursor-directed biosynthesis of curcumin analogs in Escherichia coli.
abstract: Curcuminoids, natural products in the rhizome of turmeric, show various biological activities, including antioxidant and antitumor activities. For this reason, curcuminoids have been focused on as potential pharmaceuticals. Exogenous supplementation with various carboxylate precursors in genetically engineered Escherichia coli cells carrying an artificially assembled pathway for curcuminoid biosynthesis led to the production of 17 unnatural curcuminoids.
authors.affiliation: Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Katsuyama
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Hirose
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Funa
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Sueharu
authors.initials: S
authors.lastname: Horinouchi
mesh_terms: D002264:Carboxylic Acids / Q000378:metabolism; D003373:Coumaric Acids / Q000378:metabolism; D003474:Curcumin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering; D007218:Industrial Microbiology / Q000379:methods*; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000378:metabolism; D048630:Polyketide Synthases / Q000378:metabolism; D011422:Propionates
chemical_list: D002264:Carboxylic Acids; D003373:Coumaric Acids; D011422:Propionates; D048630:Polyketide Synthases; C495469:p-coumaric acid; D003474:Curcumin