A new faecal antigen detection system for Strongyloides venezuelensis diagnosis in immunosuppressed rats.
This study was performed with the objective of developing innovative procedures for the diagnosis of strongyloidiasis. Enzyme-linked immunosorbent assay (ELISA) was conducted to detect coproantigen in the faecal samples of normal and of immunosuppressed rats using an anti-L3 polyclonal antibody produced in rabbits. Analysis revealed the kinetics of egg shedding in the non-immunosuppressed and immunosuppressed rats infected with S. venezuelensis. Further analysis verified the ability of the immune serum to detect L3 antigens in faecal samples from infected animals. The number of eggs recovered in the faeces at 8 days p.i was significantly higher for both groups. Immunosuppressed animals eliminated increased quantities of eggs. The immune serum was able to detect 0.39 microg/ml of L3 antigens. The antigen recognition in the immunosuppressed group was anticipated on the 8th day p.i. In conclusion, these results may represent a first step in the development of a rapid coproantigen detection kit for strongyloidiasis.
Gonçalves
Ana Lúcia R
AL
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará 1720, Uberlândia, Minas Gerais, Brazil.
Silva
Claudio V
CV
Ueta
Marlene T
MT
Costa-Cruz
Julia M
JM
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology*; D007106:Immune Sera / Q000276:immunology; D007165:Immunosuppression Therapy; D007700:Kinetics; D008297:Male; D010270:Parasite Egg Count; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D013321:Strongyloides / Q000276:immunology / Q000302:isolation & purification*; D013322:Strongyloidiasis / Q000175:diagnosis* / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007106:Immune Sera