title: An alternative temperature-based histamine-sensitisation test for absence of residual pertussis toxin in acellular pertussis vaccines.
abstract: As an extension of the BSP076 study (see the article 'Collaborative Study for the Standardisation of the Histamine Sensitizing Test in Mice and the CHO Cell-based Assay for the Residual Toxicity Testing of Acellular Pertussis Vaccines (BSP076)', page 51 of this issue of Pharmeuropa Bio & Scientific Notes), it was decided to publish the following experimental method for the temperature-based histamine-sensitisation test, validated at the SSI (Statens Serum Institut, Denmark), as a working basis for the growth of the method in individual laboratories.
authors.forename: S E
authors.initials: SE
authors.lastname: Jensen
authors.forename: K E
authors.initials: KE
authors.lastname: Engelhart Illigen
authors.forename: K R
authors.initials: KR
authors.lastname: Hasløv
mesh_terms: D023401:Animal Use Alternatives; D000818:Animals; D001681:Biological Assay / Q000379:methods* / Q000592:standards; D001831:Body Temperature / Q000187:drug effects; D002138:Calibration; D004305:Dose-Response Relationship, Drug; D005260:Female; D006632:Histamine / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity*; D007753:Laboratories / Q000592:standards; D051379:Mice; D008810:Mice, Inbred C57BL; D037342:Pertussis Toxin / Q000032:analysis* / Q000276:immunology; D010567:Pertussis Vaccine / Q000276:immunology / Q000592:standards* / Q000633:toxicity; D012015:Reference Standards; D013817:Thermography; D018675:Toxicity Tests / Q000379:methods* / Q000592:standards; D022282:Vaccines, Acellular / Q000276:immunology / Q000592:standards* / Q000633:toxicity
chemical_list: D010567:Pertussis Vaccine; D022282:Vaccines, Acellular; D006632:Histamine; D037342:Pertussis Toxin