Impact of leptin and leptin-receptor gene polymorphisms on serum lipids in Japanese obese children.
OBJECTIVE
Leptin is one of the factors affecting serum lipid profile. We investigated the association between serum lipids and leptin/leptin receptor (LEPR) gene polymorphisms in obese Japanese children.


METHODS
One hundred and thirty-six obese children (99 males and 37 females, relative weight over than 20%) from 5 to 17 years of age were recruited from 10 institutes. Four known polymorphisms in leptin gene [(+19)A G, (-2548)G A, (-188)C A, (-633)C T] and four known polymorphisms in LEPR gene [Lys109Arg, Gln223Arg, Pro(G)1019Pro(A), Ser(T)343Ser(C)] were determined using polymerase chain reaction-restriction fragment length polymorphism-based analyses.


RESULTS
No associations were found between leptin gene polymorphisms and serum lipid profile. On the other hand, Lys109Arg and Ser343Ser polymorphism in LEPR gene, but not Gln223Arg or Pro1019Pro, had significant relationships with serum lipid profile; lower total and low-density lipoprotein cholesterol levels in Arg109Arg homozygotes, and lower TG levels in Ser343Ser(C/C) homozygotes. In addition, LEPR gene also associated with relative weight; Arg109Arg homozygotes had higher relative weight and Ser343Ser(C/C) homozygotes had lower one.


CONCLUSIONS
These results suggest that LEPR gene polymorphisms may partly contribute to serum lipid profile in obese children.
Okada
T
T
Department of Pediatrics and Child Health, Nihon University School of Medicine, Tokyo, Japan. tomokada@med.nihon-u.ac.jp
Ohzeki
T
T
Nakagawa
Y
Y
Sugihara
S
S
Arisaka
O
O
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan; D020738:Leptin / Q000235:genetics*; D008055:Lipids / Q000097:blood*; D008297:Male; D009765:Obesity / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D054411:Receptors, Leptin / Q000235:genetics*
D020738:Leptin; D008055:Lipids; D054411:Receptors, Leptin