[Study on gene delivery via the bile duct to rat liver using naked DNA particles].
OBJECTIVE
To evaluate the efficacy of gene delivery to the right lateral lobe of the rat liver via a branch of the bile duct using naked DNA.


METHODS
We evaluated regional gene delivery to the right lateral lobe of the rat liver via a branch of the bile duct, using naked DNA, including pGL3, pCMV beta and Cy3 labeled CMV beta.


RESULTS
Gene expression was observed in right lateral lobe of both the damaged and the normal rats liver. The gene delivery efficiency was similar in two groups (P > 0.05). Gene expression was found in the right lateral lobe of damaged and normal livers. Fluorescence was observed in the region of the portal triads, and occasionally, in the lobule.


CONCLUSIONS
Retrograde infusion of naked DNA via the bile duct is an effective way to deliver genes to in both damaged and normal rat liver.
Hu
Jin-hua
JH
Liver Failure Treatment and Research Center, 302 hospital of the PLA, Beijing 100039, China. hjh@redmail.com.cn
Xu
Biao
B
Chen
Jing
J
He
Wei-ping
WP
Wang
Yie-dong
YD
Wang
Hui-fen
HF
D000818:Animals; D001652:Bile Ducts / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D005690:Galactose; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008107:Liver Diseases / Q000235:genetics / Q000473:pathology / Q000628:therapy; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D051381:Rats; D011917:Rats, Inbred Lew; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D004247:DNA; D008156:Luciferases; D001616:beta-Galactosidase; D005690:Galactose