title: Chromogranin A detection in saliva of type 2 diabetes patients.
abstract: Chromogranin A is present in secretion granules of nerve, endocrine and immune cells and is a precursor of several peptides with antibacterial and antifungal properties at micromolar concentrations.Our aim in this prospective, double blind study, was to determine the expression of chromogranin A and its peptides at protein level in saliva of type 2 diabetic patients and thereby to obtain a new non-invasive diagnostic means for the future.Saliva was taken from 30 type 2 diabetic patients and 30 healthy individuals at the same time interval in the morning without any oral stimuli. Circadianic periodics in protein productions have been avoided. The presence of chromogranin A and its derived peptides was determined in whole saliva, after centrifugation at 40C for 12 min at 14 000 rpm, by SDS-PAGE electrophoresis and Immunoblotting (Western Blot). To ensure same protein concentrations Bradford protein quantification assay has been performed before.For the first time, we have determined an overexpression of chromogranin A in saliva of diabetic patients in 100% of the individuals. Chromogranin A, a circulating biomarker for epithelial tumours, is also overexpressed in saliva of type 2 diabetic patients. To confirm our results, more studies with a large amount of patients is necessary.
authors.affiliation: Department of Periodontology, Hautepierre Hospitals, University of Strasbourg, France. INSERM Unit 977, 11 Rue Humann, 67085 Strasbourg Cedex, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Soell
authors.forename: Ahmed
authors.initials: A
authors.lastname: Feki
authors.forename: Matthias
authors.initials: M
authors.lastname: Hannig
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Sano
authors.forename: Michel
authors.initials: M
authors.lastname: Pinget
authors.forename: Denis
authors.initials: D
authors.lastname: Selimovic
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D053379:Chromogranin A / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis* / Q000378:metabolism*; D004311:Double-Blind Method; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011237:Predictive Value of Tests; D012463:Saliva / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D053379:Chromogranin A