Production of 1,3-dihydroxyacetone from glycerol by Gluconobacter oxydans ZJB09112.
The culture variables were optimized to increase 1,3-dihydroxyacetone (DHA) production by Gluconobacter oxydans ZJB09112 in shake flask and bubble column bioreactor. After fermentation in the optimized medium (g/L: yeast extract 5, glycerol 2.5, mannitol 22.5, K(2)HPO(4) 0.5, KH(2)PO(4) 0.5, MgSO(4) x 7H(2)O 0.1, CaCO(3) 2.0, pH 5.0), when five times of glycerol feeding were made, 161.9 + or - 5.9 g/l of DHA was attained at 88.7 + or - 3.2% conversion rate of glycerol to DHA.
Hu
Zhong-Ce
ZC
Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China.
Liu
Zhi-Qiang
ZQ
Zheng
Yu-Guo
YG
Shen
Yin-Chu
YC
D003470:Culture Media / Q000378:metabolism; D004098:Dihydroxyacetone / Q000378:metabolism*; D005285:Fermentation; D020600:Gluconobacter oxydans / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*
D003470:Culture Media; D004098:Dihydroxyacetone; D005990:Glycerol