In vivo analysis of mtDNA replication defects in yeast.
The yeast Saccharomyces cerevisiae has the capacity to survive large deletions or total loss of mtDNA (petite mutants), and thus in the last few years it has been used as a model system to study defects in mitochondrial DNA (mtDNA) maintenance produced by mutations in genes involved in mtDNA replication. In this paper we describe methods to obtain strains harboring mutations in nuclear genes essential for the integrity of mtDNA, to measure the frequency and the nature of petite mutants, to estimate the point mutation frequency in mtDNA and to determine whether a nuclear mutation is recessive or dominant and, in the latter case, the kind of dominance.
Baruffini
Enrico
E
Department of Genetics, Biology of Microorganisms, Anthropology, Evolution, Viale Usberti 11/A, 43124 Parma, Italy. enrico.baruffini@nemo.unipr.it
Ferrero
Iliana
I
Foury
Françoise
F
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004261:DNA Replication / Q000235:genetics*; D004271:DNA, Fungal / Q000096:biosynthesis* / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000096:biosynthesis* / Q000235:genetics*; D005456:Fluorescent Dyes; D005800:Genes, Fungal; D005821:Genetic Techniques; D007211:Indoles; D016296:Mutagenesis; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D013194:Staining and Labeling
D017931:DNA Primers; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI