title: Binding of Norovirus virus-like particles (VLPs) to human intestinal Caco-2 cells and the suppressive effect of pasteurized bovine colostrum on this VLP binding.
abstract: Noroviruses (NoVs), which cannot be grown in cell culture, are a major infectious agent of gastroenteritis. An in vitro assay system was established for the evaluation of NoV binding to enterocytes using virus-like particles (VLPs) produced in a baculovirus system expressing a NoV VP1 capsid protein. After confirmation of the purity by MS analysis, VLPs were incubated with human intestinal Caco-2 cells. NoV VLPs were detected clearly by confocal laser microscopy only on a certain population of Caco-2 cells, and were semi-quantified by immunoblotting of cell lysates. Then the suppressive effect of pasteurized bovine colostrum was analyzed on the VLP binding to Caco-2 cells by immunoblotting. The colostrum reduced VLP binding in a dose-dependent manner, at about 50% suppression with 12.5 microg of the colostral proteins. Furthermore, the colostrum contained IgG antibodies reacting to VLPs, suggesting that cross-reactive antibodies in the bovine colostrums block human NoV binding to intestinal cells.
authors.affiliation: Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Murakami
authors.forename: Sayaka
authors.initials: S
authors.lastname: Suzuki
authors.forename: Naohito
authors.initials: N
authors.lastname: Aoki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Okajima
authors.forename: Daita
authors.initials: D
authors.lastname: Nadano
authors.forename: Kenji
authors.initials: K
authors.lastname: Uchida
authors.forename: Kousaku
authors.initials: K
authors.lastname: Yamashita
authors.forename: Tomoichiro
authors.initials: T
authors.lastname: Oka
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Katayama
authors.forename: Naokazu
authors.initials: N
authors.lastname: Takeda
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001681:Biological Assay; D018938:Caco-2 Cells; D036022:Capsid Proteins / Q000276:immunology*; D002417:Cattle; D003126:Colostrum / Q000276:immunology*; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D029322:Norovirus / Q000276:immunology*; D011247:Pregnancy; D014771:Virion / Q000276:immunology*; D053585:Virus Attachment
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G