Triazophos up-regulated gene expression in the female brown planthopper, Nilaparvata lugens.
The widespread use of insecticides has caused the resurgence of the brown planthopper, Nilaparvata lugens, in Asia. In this study, we investigated an organo-phosphorous insecticide, triazophos, and its ability to induce gene expression variation in female N. lugens nymphs just before emergence. By using the suppression subtractive hybridization method, a triazophos-induced cDNA library was constructed. In total, 402 differentially expressed cDNA clones were obtained. Real-time qPCR analysis confirmed that triazophos up-regulated the expression of six candidate genes at the transcript level in nymphs on day 3 of the 5th instar. These genes encode N. lugens vitellogenin, bystin, multidrug resistance protein (MRP), purine nucleoside phosphorylase (PNP), pyrroline-5-carboxylate reductase (P5CR) and carboxylesterase. Our results imply that the up-regulation of these genes may be involved in the induction of N. lugens female reproduction or resistance to insecticides.
Bao
Yan-Yuan
YY
State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Li
Bao-Ling
BL
Liu
Zhao-Bu
ZB
Xue
Jian
J
Zhu
Zeng-Rong
ZR
Cheng
Jia-An
JA
Zhang
Chuan-Xi
CX
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D015723:Gene Library; D006430:Hemiptera / Q000187:drug effects / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity*; D009758:Nymph / Q000187:drug effects / Q000378:metabolism; D063086:Organothiophosphates / Q000633:toxicity*; D016133:Polymerase Chain Reaction; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D011762:Pyrroline Carboxylate Reductases / Q000378:metabolism; D014230:Triazoles / Q000633:toxicity*; D014819:Vitellogenins / Q000378:metabolism
D018435:ATP Binding Cassette Transporter, Subfamily B; D015815:Cell Adhesion Molecules; D017931:DNA Primers; D007306:Insecticides; D063086:Organothiophosphates; D014230:Triazoles; D014819:Vitellogenins; C012584:triazophos; D011762:Pyrroline Carboxylate Reductases; D011683:Purine-Nucleoside Phosphorylase