Concerto catalysis--harmonising [NiFe]hydrogenase and NiRu model catalysts.
This communication reports the successful merging of the chemical properties of a natural [NiFe]hydrogenase (Desulfovibrio vulgaris Miyazaki F) and our previously reported [NiRu] hydrogenase-mimic. The catalytic activity of both the natural enzyme and the mimic is almost identical, with the exception of working pH ranges, and this allows us to use them simultaneously in the same reaction flask. In such a manner, isotope exchange between D(2) and H(2)O could be conducted over an extended pH range (about 2-10) in one pot under mild conditions at ambient temperature and pressure.
Ichikawa
Koji
K
Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
Nonaka
Kyoshiro
K
Matsumoto
Takahiro
T
Kure
Bunsho
B
Yoon
Ki-Seok
KS
Higuchi
Yoshiki
Y
Yagi
Tatsuhiko
T
Ogo
Seiji
S
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D016969:Desulfovibrio vulgaris / Q000201:enzymology; D006859:Hydrogen / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006864:Hydrogenase / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D011487:Protein Conformation; D012428:Ruthenium / Q000737:chemistry*
D001426:Bacterial Proteins; D012428:Ruthenium; D006859:Hydrogen; C043296:nickel-iron hydrogenase; D006864:Hydrogenase