title: Spectral imaging reveals microvessel physiology and function from anastomoses to thromboses.
abstract: Abnormal microvascular physiology and function is common in many diseases. Numerous pathologies include hypervascularity, aberrant angiogenesis, or abnormal vascular remodeling among the characteristic features of the disease, and quantitative imaging and measurement of microvessel function can be important to increase understanding of these diseases. Several optical techniques are useful for direct imaging of microvascular function. Spectral imaging is one such technique that can be used to assess microvascular oxygen transport function with high spatial and temporal resolution in microvessel networks through measurements of hemoglobin saturation. We highlight novel observation made with our intravital microscopy spectral imaging system employed with mouse dorsal skin-fold window chambers for imaging hemoglobin saturation in microvessel networks. Specifically, we image acute oxygenation fluctuations in a tumor microvessel network, the development of arteriovenous malformations in a mouse model of hereditary hemorrhagic telangiectasia, and the formation of spontaneous and induced microvascular thromboses and occlusions.
authors.affiliation: University of Florida, College of Engineering, J. Crayton Pruitt Family Department of Biomedical Engineering, Gainesville, Florida 32611-6131, USA.
authors.forename: Mamta
authors.initials: M
authors.lastname: Wankhede
authors.forename: Nikita
authors.initials: N
authors.lastname: Agarwal
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Fraga-Silva
authors.forename: Casey
authors.initials: C
authors.lastname: deDeugd
authors.forename: Mohan K
authors.initials: MK
authors.lastname: Raizada
authors.forename: S Paul
authors.initials: SP
authors.lastname: Oh
authors.forename: Brian S
authors.initials: BS
authors.lastname: Sorg
mesh_terms: D000818:Animals; D001163:Arteriovenous Anastomosis / Q000503:physiopathology*; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008819:Mice, Nude; D055806:Microvessels / Q000502:physiology / Q000503:physiopathology; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000503:physiopathology; D010100:Oxygen / Q000378:metabolism; D012884:Skin Window Technique; D013057:Spectrum Analysis / Q000379:methods*; D013683:Telangiectasia, Hereditary Hemorrhagic; D013927:Thrombosis / Q000503:physiopathology*
chemical_list: D006454:Hemoglobins; D010100:Oxygen