title: The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular factors.
abstract: ISWI is an evolutionarily conserved ATP-dependent chromatin remodeling factor playing central roles in DNA replication, RNA transcription, and chromosome organization. The variety of biological functions dependent on ISWI suggests that its activity could be highly regulated. Our group has previously isolated and characterized new cellular activities that positively regulate ISWI in Drosophila melanogaster. To identify factors that antagonize ISWI activity we developed a novel in vivo eye-based assay to screen for genetic suppressors of ISWI. Our screen revealed that ISWI interacts with an evolutionarily conserved network of cellular and nuclear factors that escaped previous genetic and biochemical analyses.
authors.affiliation: Dipartimento di Scienze Biochimiche, Istituto Telethon Dulbecco, Universitá degli Studi di Palermo, Palermo, Italy.
authors.forename: Walter
authors.initials: W
authors.lastname: Arancio
authors.forename: Maria C
authors.initials: MC
authors.lastname: Onorati
authors.forename: Giosalba
authors.initials: G
authors.lastname: Burgio
authors.forename: Marianna
authors.initials: M
authors.lastname: Collesano
authors.forename: Antonia M R
authors.initials: AM
authors.lastname: Ingrassia
authors.forename: Swonild I
authors.initials: SI
authors.lastname: Genovese
authors.forename: Manolis
authors.initials: M
authors.lastname: Fanto
authors.forename: Davide F V
authors.initials: DF
authors.lastname: Corona
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001681:Biological Assay; D002453:Cell Cycle; D017124:Conserved Sequence; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D019143:Evolution, Molecular; D005123:Eye / Q000473:pathology; D005470:Fluorometry; D016153:Genes, Suppressor; D005820:Genetic Testing; D009154:Mutation / Q000235:genetics; D009707:Nucleosomes / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; C086468:ISWI protein; D009707:Nucleosomes; D014157:Transcription Factors; D000251:Adenosine Triphosphatases