Evaluation of holocellulase production by plant-degrading fungi grown on agro-industrial residues.
Agaricus brasiliensis CS1, Pleurotus ostreatus H1 and Aspergillus flavus produced holocellulases when grown in solid and submerged liquid cultures containing agro-industrial residues, including sugar cane bagasse and dirty cotton residue, as substrates. These isolates proved to be efficient producers of holocellulases under the conditions used in this screening. Bromatological analysis of agro-industrial residues showed differences in protein, fiber, hemicellulose, cellulose and lignin content. Maximal holocellulase activity (hemicellulase, cellulase and pectinase) was obtained using solid-state cultivation with 10% substrate concentration. In this case, remarkably high levels of xylanase and polygalacturonase activity (4,008 and 4,548 IU/l, respectively) were produced by A. flavus when grown in media containing corn residue, followed by P. ostreatus H1 with IU/l values of 1,900 and 3,965 when cultivated on 5% and 10% sugar cane bagasse, respectively. A. brasiliensis CS1 showed the highest reducing sugar yield (11.640 mg/ml) when grown on medium containing sugar cane bagasse. A. brasiliensis was also the most efficient producer of protein, except when cultivated on dirty cotton residue, which induced maximal production in A. flavus. Comparison of enzymatic hydrolysis of sugar cane bagasse and dirty cotton residue by crude extracts of A. brasiliensis CS1, P. ostreatus H1 and A. flavus showed that the best reducing sugar yield was achieved using sugar cane bagasse as a substrate.
de Siqueira
Félix Gonçalves
FG
Department of Cellular Biology, University of Brasília, Brasília, Brazil.
de Siqueira
Aline Gonçalves
AG
de Siqueira
Eliane Gonçalves
EG
Carvalho
Marly Azevedo
MA
Peretti
Beatriz Magalhães Pinto
BM
Jaramillo
Paula Marcela Duque
PM
Teixeira
Ricardo Sposina Sobral
RS
Dias
Eustáquio Souza
ES
Félix
Carlos Roberto
CR
Filho
Edivaldo Ximenes Ferreira
EX
D000364:Agaricus / Q000201:enzymology / Q000254:growth & development; D000383:Agriculture; D001231:Aspergillus flavus / Q000201:enzymology / Q000254:growth & development; D002241:Carbohydrates / Q000032:analysis; D002480:Cellulase / Q000096:biosynthesis* / Q000378:metabolism; D045424:Complex Mixtures; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D005656:Fungal Proteins / Q000032:analysis; D005658:Fungi / Q000201:enzymology* / Q000254:growth & development*; D006868:Hydrolysis; D007220:Industrial Waste; D010084:Oxidation-Reduction; D010944:Plants / Q000382:microbiology*; D020076:Pleurotus / Q000201:enzymology / Q000254:growth & development
D002241:Carbohydrates; D045424:Complex Mixtures; D005656:Fungal Proteins; D007220:Industrial Waste; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases