Immunostimulatory effects of extract of Pulicaria crispa before and after Schistosoma mansoni infection.
The immunostimulatory effects of methanolic extract from Pulicaria crispa were investigated in mice before and after infection with Schistosoma mansoni. Mice were subjected for daily intra-peritoneal injection by the extract (33 ng/mouse) for 10 successive days followed by infecting every mouse with 100 S. mansoni cercariae. Treatment with the extract induced significant increase (p < 0.05) in sera-IL-2 before and after infection. Upon using soluble worm antigen preparation or cancer bladder homogenates as antigens in ELISA, the detected levels of IgG were significantly (p < 0.05) higher in sera from treated-infected mice than untreated P. crispa infected mice. Using crude Escherichia coli lysate as an antigen in ELISA, it was detected a significant (p < 0.05) increase in IgG levels in sera from the extract-treated mice before and after infection.
Maghraby
Amany S
AS
Therapeutic Chemistry Department, National Research Center, Dokki, Cairo 12311, Egypt.
Shalaby
Nagwa
N
Abd-Alla
Howida I
HI
Ahmed
Samia A
SA
Khaled
Hussein M
HM
Bahgat
Mahmoud M
MM
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007274:Injections, Intraperitoneal; D007376:Interleukin-2 / Q000097:blood; D051379:Mice; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D052587:Pulicaria; D012550:Schistosoma mansoni / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000188:drug therapy* / Q000276:immunology / Q000469:parasitology; D012556:Schistosomicides / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000276:immunology
D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007376:Interleukin-2; D010936:Plant Extracts; D012556:Schistosomicides