[The effects of AML1B and AML1/ETO on the transactivation of TSC genes.].
OBJECTIVE
To investigate the effects of AML1B and AML1/ETO fusion gene on the transcription activity of TSC1 and TSC2 promotor and to explore its role in leukemogenesis.


METHODS
The luciferase reporter plasmids of TSCs gene promoter containing a AML1 binding site were constructed, and cotransfected into CV-1 cells with AML1B or AML1/ETO expression plasmids separately. The transactivity of TSCs gene promoter was assayed by luminometer.


RESULTS
AML1B exhibited a transactivity to TSC2 gene promoter in a dosage-dependant manner, but showed no significant transactivity to TSC1's. The transactivity to TSC2 gene promoter showed 8.55 fold increase as companed with control group at 75 ng of pCMV5-AML/B. AML1/ETO showed a significant transactivity to TSC1, but no transactivity to TSC2's. However, AML1/ETO antagonized the effect of AMLlB to TSC2 gene promoter.


CONCLUSIONS
AML1B and AML1/ETO can regulate the transcription of TSC genes.
Xu
Zhi-Fang
ZF
State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
Wang
Lin
L
Wang
Yang
Y
Wang
Min
M
Rao
Qing
Q
Xing
Hai-Yan
HY
Wang
Jian-Xiang
JX
D001665:Binding Sites; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics; D006801:Humans; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics; D015533:Transcriptional Activation
D050676:Core Binding Factor Alpha 2 Subunit; D015514:Oncogene Proteins, Fusion; D014157:Transcription Factors