title: [Epidemiological investigation on natural infection of different canine breeds with Borna disease virus in Ili, China].
abstract: OBJECTIVE
  To investigate the epidemiological pattern of Borna disease virus (BDV) among different canine breeds in Ili, China, and to analyze its potential phylogeny.
  
  
  METHODS
  BDV p24 RNA fragments were detected from peripheral blood mononuclear cells (PBMCs) of canine by modified nested RT-PCR (nRT-PCR). Possible false positives were excluded by determination of both BDV p40 RNA fragments and PMD19 plasmid standards. Analysis were performed on genetic sequence, homologous comparison, amino acid sequence and phylogeny after p24 positive products were validated.
  
  
  RESULTS
  BDV p24 RNA fragments were found only in Kazakh Tobet (a shepherd dog) in 8 breeds of 150 cases and their overall positive rate was 11.0% (10/91). Compared with the strain of He/80 from horse and that of S6 from sheep in Germany, the homologous similarities of Kazakh Tobet was 99.2% and 95.7%, and that of amino acid as 100% and 89.3%, respectively. The kinship of Kazakh Tobet was close to He/80 and next to S6.
  
  
  CONCLUSIONS
  There was potential natural BDV infection in Kazakh Tobet in Ili, and its endemic strain was concerned with He/80 infecting Ili horse and S6 of German Merino sheep introduced into the region from Germany.
authors.affiliation: Department of Neurology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Qun-ling
authors.initials: QL
authors.lastname: Zhan
authors.forename: Ying-ying
authors.initials: YY
authors.lastname: Zhang
authors.forename: Ming-ming
authors.initials: MM
authors.lastname: Xu
authors.forename: Yong-bo
authors.initials: YB
authors.lastname: Hu
authors.forename: Xiu-ling
authors.initials: XL
authors.lastname: Wu
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Yu
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.forename: De-yu
authors.initials: DY
authors.lastname: Yang
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001890:Borna Disease / Q000453:epidemiology* / Q000821:virology; D001891:Borna disease virus / Q000235:genetics / Q000302:isolation & purification*; D002681:China / Q000453:epidemiology; D004285:Dogs / Q000821:virology*; D005858:Germany / Q000453:epidemiology; D006734:Horse Diseases / Q000453:epidemiology / Q000821:virology; D006736:Horses / Q000821:virology; D007963:Leukocytes, Mononuclear / Q000821:virology; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D012367:RNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep / Q000821:virology; D012757:Sheep Diseases / Q000453:epidemiology / Q000821:virology; D013045:Species Specificity
chemical_list: D012367:RNA, Viral