A cell-free protein synthesis system from insect cells.
The Transdirect insect cell is a newly developed in vitro translation system for mRNA templates, which utilizes an extract from cultured Spodoptera frugiperda 21 (Sf21) insect cells. An expression vector, pTD1, which includes a 5'-untranslated region (UTR) sequence from a baculovirus polyhedrin gene as a translational enhancer, was also developed to obtain maximum performance from the insect cell-free protein synthesis system. This combination of insect cell extract and expression vector results in protein productivity of about 50 microg/mL of the translation reaction mixture. This is the highest protein productivity yet noted among commercialized cell-free protein synthesis systems based on animal extracts.
Ezure
Toru
T
Clinical & Biotechnology Business Unit, Analytical & Measuring Instruments Division, Life Science Business Department, Shimadzu Corporation, Kyoto, Japan.
Suzuki
Takashi
T
Shikata
Masamitsu
M
Ito
Masaaki
M
Ando
Eiji
E
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D002474:Cell-Free System; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D015202:Protein Engineering / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis*; D018411:Spodoptera / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D011994:Recombinant Proteins