Flow cytometry vs cytomorphology for the detection of hematologic malignancy in body cavity fluids.
Flow cytometry and cytomorphology results on 92 body cavity fluids [61 effusions and 31 bronchoalveolar lavage fluids (BALF)] from hematologic malignancy were compared with retrospective clinical outcome. We observed double true positive/negative results in 67 cases (73%), and double false negative results in 2 cases (2%). Immunophenotyping accounted for true positive/negative results in 22 out of 23 mismatched cases (25%), and retained significantly higher accuracy than that of cytomorphology especially in effusions and differentiated lymphoma. In BALF analysis, immunophenotyping and cytomorphology sensitivity was 75% and 0%, respectively. Flow cytometry retains the highest accuracy in detecting neoplastic cells in body cavity fluids.
Cesana
Clara
C
Department of Transfusion Medicine, Division of Hematology, Ospedale Niguarda Ca' Granda Hospital, Piazza Ospedale Maggiore, 3, 20162 Milan, Italy.
Klersy
Catherine
C
Scarpati
Barbara
B
Brando
Bruno
B
Volpato
Elisabetta
E
Bertani
Giambattista
G
Faleri
Maurizio
M
Nosari
Annamaria
A
Cantoni
Silvia
S
Ferri
Ursula
U
Scampini
Linda
L
Barba
Claudia
C
Lando
Giuliana
G
Morra
Enrica
E
Cairoli
Roberto
R
D000368:Aged; D014408:Biomarkers, Tumor; D001826:Body Fluids; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002452:Cell Count; D005189:False Positive Reactions; D005260:Female; D005434:Flow Cytometry; D019337:Hematologic Neoplasms / Q000175:diagnosis*; D006801:Humans; D016130:Immunophenotyping; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012189:Retrospective Studies
D014408:Biomarkers, Tumor