title: [Leukocyte stimulation by DNA fragments shored up protamine in cyclophosphamide-induced leukopoiesis in mice].
abstract: Our study showed that protamine (80% w/w to DNA) effectively protected its molecules from degradation by native nucleases of the mammalian blood serum. Exogenous DNA bound to protamine effectively stimulated restoration of cyclophosphamide-induced leukopoiesis in mice. It is suggested that the phenomenon was due to repair processes taking place in hemopoietic stem cells damaged by a cross-linking cytostatic drug such as cyclophosphamide. DNA dosage may be reduced and the original DNA fragment size maintained by DNA binding to protamine. As a result, it might involve longer DNA fragments into repair processes of homologous recombination and eventually increase the cell's chances of getting rid of extensive damage.
authors.forename: E V
authors.initials: EV
authors.lastname: Dolgova
authors.forename: V A
authors.initials: VA
authors.lastname: Rogachev
authors.forename: V P
authors.initials: VP
authors.lastname: Nikolin
authors.forename: N A
authors.initials: NA
authors.lastname: Popova
authors.forename: A S
authors.initials: AS
authors.lastname: Likhacheva
authors.forename: E A
authors.initials: EA
authors.lastname: Aliamkina
authors.forename: T E
authors.initials: TE
authors.lastname: Sebeleva
authors.forename: E R
authors.initials: ER
authors.lastname: Chernykh
authors.forename: E L
authors.initials: EL
authors.lastname: Gel'fgat
authors.forename: S S
authors.initials: SS
authors.lastname: Bogachev
authors.forename: M A
authors.initials: MA
authors.lastname: Shudrov
mesh_terms: D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D019891:Leukopoiesis / Q000187:drug effects* / Q000235:genetics; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011479:Protamines / Q000378:metabolism*
chemical_list: D007166:Immunosuppressive Agents; D011479:Protamines; D003520:Cyclophosphamide