Screening of Pseudomonas and Bacillus isolates for potential biocontrol of the damping-off of bean (Phaseolus coccineus).
In this study fifteen isolates of identified Pseudomonas fluorescens and Bacillus subtilis were investigated for control of bean damping-off disease caused by Rhizoctonia solani. In vitro, P. fluorescens P-6 and B. subtilis B-3 showed the most inhibitory zone in dual culture assay against R. solani. The growth of P-6 (4.5 x 10(8) cfu/ml) was significantly higher than in the other treatments. In greenhouse condition, all of the isolates effectively controlled damping-off on bean. P. fluorescens P-5 and P-6 showed the considerable results against R. solani and could reduce the damping-off disease from 100% to less than 30%. P-5, P-6 (P. fluorescens) and 8-16 (8. subtilis) strains had the highest effect on fresh weight of bean.
Peighami-Ashnaei
S
S
Department of Plant Protection, Faculty of Horticultural Science & Plant Protection University of Tehran, Karaj, Iran.
Sharifi-Tehrani
A
A
Ahmadzadeh
M
M
Behboudi
K
K
D001407:Bacillus / Q000302:isolation & purification* / Q000472:pathogenicity; D010571:Pest Control / Q000379:methods; D010572:Pest Control, Biological / Q000379:methods; D027805:Phaseolus / Q000254:growth & development / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D018520:Plant Shoots / Q000254:growth & development / Q000382:microbiology; D011549:Pseudomonas / Q000302:isolation & purification* / Q000472:pathogenicity; D011551:Pseudomonas fluorescens / Q000302:isolation & purification / Q000472:pathogenicity