The effects of radix curcumae extract on expressions of VEGF, COX-2 and PCNA in gastric mucosa of rats fed with MNNG.
OBJECTIVE
To study the effects of an extract solution from radix curcumae on the expressions of VEGF, COX-2 and PCNA in gastric mucosa of rats during carcinogenesis induced MNNG.


METHODS
Eighty male Wistar rats were randomly divided into 5 groups: group A, water and normal saline; group B, MNNG and normal saline; group C, MNNG and celecoxib; group D, MNNG and low-dose (1 g/ml) radix curcumae steam distillation extract solution; group E, MNNG and high-dose (2g/ml) radix curcumae wet distillation extract solution. In the end of the 40(th) week, all rats alive were sacrificed and the expressions of VEGF, COX-2 and PCNA in gastric mucosa were determined by immunohistochemistry.


RESULTS
The expression levels of VEGF and COX-2 and the optical density levels of PCNA in group C, D and E were remarkably lower than that of group B (P<0.05), while the optical density levels of PCNA in group E were higher than that of group C and D (P<0.05).


CONCLUSIONS
The distilled extract of curcumae can down-regulate the expressions of VEGF, COX-2 and PCNA in the gastric mucosa of rats during carcinogenesis induced MNNG and can reduce the incidence of gastric caner, suggesting it maybe as a potential chemopreventive agent for gastric cancer.
Lu
Bin
B
Department of Gastroenterology, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, China.
Yu
Linfeng
L
Xu
Lei
L
Chen
Hanqing
H
Zhang
Lu
L
Zeng
Yanjun
Y
D000284:Administration, Oral; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage; D002273:Carcinogens; D030024:Curcuma / Q000737:chemistry*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D008769:Methylnitronitrosoguanidine; D010936:Plant Extracts / Q000008:administration & dosage*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D016896:Treatment Outcome; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D002273:Carcinogens; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A; D008769:Methylnitronitrosoguanidine; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2