[Hypoxia promotes corpus cavernosum smooth muscle cell apoptosis in SD rats].
OBJECTIVE
To explore the relationship between hypoxia and the apoptosis of corpus cavernosum smooth muscle cells (CCSMC) in SD rats.


METHODS
CCSMCs were cultured in vitro and identified by immunohistochemistry, and then underwent hypoxia interference at the concentration of 1% O2 for 12, 24, 48 and 72 hours, with normal oxygen concentration as the control. Flow cytometry was used to determine the cycles and apoptosis of the cells.


RESULTS
The cultured CCSMCs grew well, positive for anti-smooth muscle alpha-actin monoclonal antibody immunohistochemical staining. Flow cytometry showed that the number of CCSMCs in G0/G1 was gradually increased within 48 hours and then decreased, just opposite to the proportion of the S phase cells. But no regular change was found in the proportion of the cells in the G2/M phase.


CONCLUSIONS
Hypoxia promotes the apoptosis of CCSMCs in a time-dependent manner, to the maximum at 48 hours, and then cell lysis may occur, but with no further apoptosis.
Lü
Bo-Dong
BD
Department of Urology, The Second Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310005, China.
Nian
Jian-Hua
JH
Huang
Xiao-Jun
XJ
Zhang
Shi-Geng
SG
Geng
Qiang
Q
D000818:Animals; D017209:Apoptosis; D015687:Cell Hypoxia; D002478:Cells, Cultured; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D010413:Penis / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley