title: Effect of plant growth regulators and genotype on the micropropagation of adult trees of Arbutus unedo L. (strawberry tree).
abstract: Arbutus unedo grows spontaneously around the Mediterranean basin. The species is tolerant to drought and has a strong regeneration capacity following fires making it interesting for Mediterranean forestation programs. Considering the sparse information about the potential of this fruit tree to be propagated in vitro, a project to clone selected trees based on their fruit production was initiated a few years ago. The role of several factors on A. unedo propagation was evaluated. The results showed that 8.9 μm kinetin gave the best results although not significantly different from those obtained with benzyladenine or zeatin. The inclusion of thidiazuron or 1-naphthaleneacetic acid promoted callus growth and had deleterious effects on the multiplication rate. The genotype of the donor plants is also a factor interfering with the multiplication. The results also indicated that the conditions used for multiplication influenced the behavior of shoots during the rooting phase.
authors.affiliation: CERNAS, D. Florestal, Escola Superior Agrária de Coimbra, Bencanta, 3040-316 Coimbra, Portugal. fgomes@esac.pt
authors.forename: Filomena
authors.initials: F
authors.lastname: Gomes
authors.forename: Mafalda
authors.initials: M
authors.lastname: Simões
authors.forename: Maria L
authors.initials: ML
authors.lastname: Lopes
authors.forename: Jorge M
authors.initials: JM
authors.lastname: Canhoto
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D029791:Ericaceae / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D005838:Genotype; D007701:Kinetin / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010671:Phenylurea Compounds / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D012038:Regeneration / Q000187:drug effects; D013830:Thiadiazoles / Q000494:pharmacology; D014197:Trees / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015026:Zeatin / Q000494:pharmacology
chemical_list: D009280:Naphthaleneacetic Acids; D010671:Phenylurea Compounds; D010937:Plant Growth Regulators; D013830:Thiadiazoles; C016785:thidiazuron; C034182:1-naphthaleneacetic acid; D015026:Zeatin; D000225:Adenine; D007701:Kinetin