Altered brain myelin sheath morphology after rewarming in situ.
In this study cerebral ultrastructure was examined in an in vivo rat model, after rewarming from profound hypothermia (15-13 degrees C). Animals held at 37 degrees C served as controls. After rewarming, brains were examined by electron microscope. Micrographs were taken randomly, analyzed anonymously, and quantified by morphometry. Serum analysis of the stress marker S-100beta was carried out in identical groups. The most striking findings in rewarmed animals, when compared to controls, were alterations of myelin sheaths (p<.008) and elevated S-100beta (p<.0001). This indicates that cells in the central nervous system are susceptible to injury in an experimental model of accidental hypothermia and rewarming.
Dietrichs
E S
ES
Department of Anesthesiology, Institute of Clinical Medicine, University of Tromsø, Tromsø, Norway.
Lindal
S
S
Naesheim
T
T
Ingebrigtsen
T
T
Tveita
T
T
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001930:Brain Injuries / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D054022:Cerebrum / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D006439:Hemodynamics; D007036:Hypothermia, Induced / Q000009:adverse effects*; D008297:Male; D046529:Microscopy, Electron, Transmission; D009186:Myelin Sheath / Q000648:ultrastructure*; D009414:Nerve Growth Factors / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017694:Rewarming; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism
D015415:Biomarkers; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins