Tumors induced by Murine herpesvirus 60 or by cell line NB-78 derived from a tumor induced by Murine herpesvirus 78 show presence of the inducing viruses.
Murine herpesviruses 60 and 78 (MHV-60, MHV-78), closely related to Mouse herpesvirus strain 68 (MHV-68), are oncogenic lymphotropic gammaherpesviruses, which may serve as models for study of human oncogenic gammaherpesviruses such as Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV). In this work, we attempted to detect an analog of the MHV-68 ORF73 gene in tumors induced in mice either directly by MHV-60 or indirectly by MHV-78 via inoculation of NB-78 cells derived from a tumor induced by MHV-78. Besides the ORF73 gene, viral antigen and infectious virus were searched for. We succeeded in inducing lymphomas in mice by NB-78 cells and thus confirmed their transformed character. Importantly, we showed that the tumors induced by either MHV-60 or NB-78 cells were positive for the ORF73 gene, viral antigen and infectious virus. These results confirmed the generally accepted hypothesis about the connection between reactivation of latent gammaherpesviruses and malignant tumorigenesis.
Hrabovská
Z
Z
Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic.
Chalupková
A
A
Mistríková
J
J
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D045744:Cell Line, Tumor; D018142:Gammaherpesvirinae / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity; D006566:Herpesviridae Infections / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000821:virology*; D016366:Open Reading Frames / Q000235:genetics; D014412:Tumor Virus Infections / Q000821:virology*
D000956:Antigens, Viral