title: The winged-helix transcription factor JUMU regulates development, nucleolus morphology and function, and chromatin organization of Drosophila melanogaster.
abstract: The PEV-modifying winged-helix/forkhead domain transcription factor JUMU of Drosophila is an essential protein of pleiotropic function. The correct gene dose of jumu is required for nucleolar integrity and correct nucleolus function. Overexpression of jumu results in bloating of euchromatic chromosome arms, displacement of the JUMU protein from the chromocenter and the nucleolus, fragile weak points, and disrupted chromocenter of polytene chromosomes. Overexpression of the acidic C terminus of JUMU alone causes nucleolus disorganization. In addition, euchromatic genes are overexpressed and HP1, which normally accumulates in the pericentric heterochromatin and spreads into euchromatic chromosome arms, although H3-K9 di-methylation remains restricted to the pericentric heterochromatin. The human winged-helix nude gene shows similarities to jumu and its overexpression in Drosophila causes bristle mutations.
authors.affiliation: Institut für Biologie-Genetik, Freie Universität Berlin, Takustr. 6, 14195, Berlin, Germany. hofmann@genetik.fu-berlin.de
authors.forename: Annemarie
authors.initials: A
authors.lastname: Hofmann
authors.forename: Madeleine
authors.initials: M
authors.lastname: Brünner
authors.forename: Alexander
authors.initials: A
authors.lastname: Schwendemann
authors.forename: Martin
authors.initials: M
authors.lastname: Strödicke
authors.forename: Sascha
authors.initials: S
authors.lastname: Karberg
authors.forename: Ansgar
authors.initials: A
authors.lastname: Klebes
authors.forename: Harald
authors.initials: H
authors.lastname: Saumweber
authors.forename: Günter
authors.initials: G
authors.lastname: Korge
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002466:Cell Nucleolus / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000254:growth & development* / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D011485:Protein Binding; D021381:Protein Transport; D012469:Salivary Glands / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D029721:Drosophila Proteins; D014157:Transcription Factors; C412163:jumu protein, Drosophila