The blood-brain barrier penetration and distribution of PEGylated fluorescein-doped magnetic silica nanoparticles in rat brain.
PEGylated PAMAM conjugated fluorescein-doped magnetic silica nanoparticles (PEGylated PFMSNs) have been synthesized for evaluating their ability across the blood-brain barrier (BBB) and distribution in rat brain. The obtained nanoparticles were characterized by transmission electron microscopy (TEM), thermal gravimetry analyses (TGA), zeta potential (zeta-potential) titration, and X-ray photoelectron spectroscopy (XPS). The BBB penetration and distribution of PEGylated PFMSNs and FMSNs in rat brain were investigated not only at the cellular level with Confocal laser scanning microscopy (CLSM), but also at the subcellular level with transmission electron microscopy (TEM). The results provide direct evidence that PEGylated PFMSNs could penetrate the BBB and spread into the brain parenchyma.
Ku
Shuting
S
School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing, China.
Yan
Feng
F
Wang
Ying
Y
Sun
Yilin
Y
Yang
Nan
N
Ye
Ling
L
D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism*; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D050091:Dendrimers / Q000493:pharmacokinetics; D052203:Ferrosoferric Oxide / Q000493:pharmacokinetics*; D016650:Fluorescein-5-isothiocyanate / Q000493:pharmacokinetics*; D008297:Male; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D011092:Polyethylene Glycols / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000493:pharmacokinetics*
D050091:Dendrimers; C104700:PAMAM Starburst; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D016650:Fluorescein-5-isothiocyanate; D052203:Ferrosoferric Oxide