Pretreatment by ultra-high pressure explosion with homogenizer facilitates cellulase digestion of sugarcane bagasses.
Sugarcane bagasse was treated with ultra-high pressure explosion (UHPE) in a homogenizer. UHPE significantly decreased the particle size and disrupt the microstructure of bagasse, which was changed from a rigid and highly ordered fibril to a distorted, poly-porous and "empty-inside" structure. UHPE decreased the crystallinity indexes (CrIs) from 54.8% (0 MPa) to 45.3% (100 MPa), and increased the accessible surface area (ASA) of bagasse nearly 3-fold. The effect of UHPE on composition was minor, only decreased the lignin slightly. However, UHPE increased enzymatic digestibility substantially from 29.5% (0 MPa) to 59.4% (100 MPa), and the microstructure disruption and thereby ASA increase were found to be the major factors to enhance the digestibility. UHPE combination with treatment of 0.5% NaOH at 125 degrees C for 120 min exhibited a significant delignification and increased enzymatic digestibility to 95.5% with 5.8 FPU cellulase/g solid, implying that UHPE combined with alkaline pretreatment can be a potential alternative for biomass pretreatment in biofuel production.
Chen
Dong
D
College of Life Science and Biotechnology, Guangxi University, 100 University Road, Nanning, Guangxi 530004, China.
Guo
Yuan
Y
Huang
Ribo
R
Lu
Qi
Q
Huang
Jun
J
D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D003460:Crystallization; D004798:Enzymes / Q000737:chemistry; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010316:Particle Size; D011312:Pressure; D031786:Saccharum / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D004798:Enzymes; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D002480:Cellulase