title: The resorption of nanocrystalline calcium phosphates by osteoclast-like cells.
abstract: Nanocrystalline calcium phosphates containing carbonate have a high similarity to bone mineral. The reactions of bone cells (primary osteoblasts and osteoclast-like cells) on these materials as well as on sintered beta-tricalcium phosphate and hydroxyapatite (HA) confirmed a good biocompatibility of the nanocrystalline samples. However, osteoclastic differentiation was constrained on the carbonate-rich samples, leading to a small number of osteoclast-like cells on the materials and few resorption pits. The grain size of the calcium phosphate ceramics (nano vs. micro) was less important than expected from to physico-chemical considerations. When comparing the nanocrystalline samples, the highest resorption rate was found for nano-HA with a low carbonate content, which strongly stimulated the differentiation of osteoclast-like cells on its surface.
authors.affiliation: BioCer EntwicklungsGmbH, Ludwig-Thoma-Str. 36c, 95447 Bayreuth, Germany. rainer.detsch@biocer-gmbh.de
authors.forename: R
authors.initials: R
authors.lastname: Detsch
authors.forename: D
authors.initials: D
authors.lastname: Hagmeyer
authors.forename: M
authors.initials: M
authors.lastname: Neumann
authors.forename: S
authors.initials: S
authors.lastname: Schaefer
authors.forename: A
authors.initials: A
authors.lastname: Vortkamp
authors.forename: M
authors.initials: M
authors.lastname: Wuelling
authors.forename: G
authors.initials: G
authors.lastname: Ziegler
authors.forename: M
authors.initials: M
authors.lastname: Epple
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000378:metabolism; D002478:Cells, Cultured; D007527:Isoenzymes / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D012886:Skull / Q000166:cytology; D000071681:Tartrate-Resistant Acid Phosphatase; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002256:Carbonic Anhydrases