title: Sterol effect on interaction between amphidinol 3 and liposomal membrane as evidenced by surface plasmon resonance.
abstract: The affinity of amphidinol 3 (AM3) to phospholipid membranes in the presence and absence of sterol was examined by surface plasmon resonance (SPR) experiments. The results showed that AM3 has 1000 and 5300 times higher affinity for cholesterol- and ergosterol-containing liposomes, respectively, than those without sterol. The two-state reaction model well reproduced the sensor grams, which indicated that the interaction is composed of two steps, which correspond to binding to the membrane and internalization to form stable complexes.
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Respati T
authors.initials: RT
authors.lastname: Swasono
authors.forename: Ryota
authors.initials: R
authors.lastname: Mouri
authors.forename: Nagy
authors.initials: N
authors.lastname: Morsy
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Matsumori
authors.forename: Tohru
authors.initials: T
authors.lastname: Oishi
authors.forename: Michio
authors.initials: M
authors.lastname: Murata
mesh_terms: D000475:Alkenes / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D004875:Ergosterol / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D011714:Pyrans / Q000737:chemistry*; D013261:Sterols / Q000737:chemistry; D020349:Surface Plasmon Resonance; D000095722:Amphidinols
chemical_list: D000475:Alkenes; D002784:Cholesterol; D004875:Ergosterol; D008081:Liposomes; D011714:Pyrans; D013261:Sterols; C438973:amphidinol 3; D000095722:Amphidinols