Synthesis, in-vitro antimicrobial and cytotoxic studies of novel azetidinone derivatives.
Developing novel antimicrobial drugs is increasingly important in the modern pharmaceutical industry. A series of novel 3-chloro-4-[4-(2-oxo-2H-chromen-4-ylmethoxy)phenyl]-1-phenylazetidin-2-ones 5a-o have been synthesized from 4-bromomethylcoumarins 1a-e and 4-aryliminomethyl-phenols 3a-c. These compounds were screened for their in-vitro antibacterial activity against two Gram-positive (Staphylococcus aureus and Vancomycin resistant enteroccoccus) and two Gram-negative (Escherichia coli and Shigella dysentery) bacterial strains and antifungal activity against Aspergillus fumigatus, Candida albicans, and Penicillium. Results revealed that compounds 5c, 5f, 5h, 5j, and 5m showed excellent activity against a panel of microorganisms. The brine-shrimp bioassay was also carried out to study their in-vitro cytotoxic properties and two compounds, 5h and 5m, possessing LD(50) = 7.154x10(-4 )M and 5.782x10(-4) M, respectively, displayed potent cytotoxic activity against Artemia salina. The presence of a chlorine group in the coumarin moiety, its effect on their antibacterial, antifungal, and cytotoxic activities is discussed. All newly synthesized compounds were characterized by elemental analysis, IR, (1)H-NMR,( 13)C-NMR, and MS.
Keri
Rangappa S
RS
P. G. Department of Studies in Chemistry, Karnatak University, Pavate Nagar, Dharwad, India.
Hosamani
Kallappa M
KM
Reddy
Harisha S
HS
Shingalapur
Ramya V
RV
D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology / Q000633:toxicity; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000494:pharmacology / Q000633:toxicity; D001156:Artemia / Q000187:drug effects; D001384:Azetidines / Q000138:chemical synthesis / Q000494:pharmacology / Q000633:toxicity; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D001681:Biological Assay; D052978:Disk Diffusion Antimicrobial Tests; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D007928:Lethal Dose 50; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D013055:Spectrophotometry, Infrared; D013329:Structure-Activity Relationship
C116379:2-azetidinone; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001384:Azetidines