Biocompatibility and antibacterial effect of silver doped 3D-glass-ceramic scaffolds for bone grafting.
A 3D-glass-ceramic scaffold for bone tissue engineering with an interconnected macroporous network of pores was doped with silver ions in order to confer antibacterial properties. For this purpose, silver ions were selectively added to the scaffold surfaces through ion-exchange using an aqueous silver nitrate solution. The silver-doped scaffolds were characterized by means of leaching, in vitro antibacterial, and citotoxicity tests. In particular, the silver effect was examined through a broth dilution test in order to evaluate the proliferation of bacteria by counting the colonies forming units. Moreover, cytotoxicity tests were carried out to understand the effect of silver-containing scaffolds on cell adhesion, proliferation, and vitality. For all tests a comparison between silver-doped scaffold and silver-doped scaffold dry sterilized was performed.
Balagna
Cristina
C
Politecnico di Torino, Materials Science and Chemical Engineering Department, Corso Duca degli Abruzzi 24,10129 Turin, Italy.
Vitale-Brovarone
Chiara
C
Miola
Marta
M
Verné
Enrica
E
Canuto
Rosa Angela
RA
Saracino
Silvia
S
Muzio
Giuliana
G
Fucale
Giacomo
G
Maina
Giovanni
G
D000891:Anti-Infective Agents, Local / Q000032:analysis / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000494:pharmacology*; D016025:Bone Transplantation / Q000379:methods*; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D005898:Glass / Q000737:chemistry*; D007474:Ion Exchange; D008422:Materials Testing / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D012834:Silver / Q000494:pharmacology; D012835:Silver Nitrate / Q000032:analysis / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction / Q000379:methods
D000891:Anti-Infective Agents, Local; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D012834:Silver; D012835:Silver Nitrate