Stepwise assembly of dimeric F(1)F(o)-ATP synthase in mitochondria involves the small F(o)-subunits k and i.
F(1)F(o)-ATP synthase is a key enzyme of oxidative phosphorylation that is localized in the inner membrane of mitochondria. It uses the energy stored in the proton gradient across the inner mitochondrial membrane to catalyze the synthesis of ATP from ADP and phosphate. Dimeric and higher oligomeric forms of ATP synthase have been observed in mitochondria from various organisms. Oligomerization of ATP synthase is critical for the morphology of the inner mitochondrial membrane because it supports the generation of tubular cristae membrane domains. Association of individual F(1)F(o)-ATP synthase complexes is mediated by the membrane-embedded F(o)-part. Several subunits were mapped to monomer-monomer-interfaces of yeast ATP synthase complexes, but only Su e (Atp21) and Su g (Atp20) have so far been identified as crucial for the formation of stable dimers. We show that two other small F(o)-components, Su k (Atp19) and Su i (Atp18) are involved in the stepwise assembly of F(1)F(o)-ATP synthase dimers and oligomers. We have identified an intermediate form of the ATP synthase dimer, which accumulates in the absence of Su i. Moreover, our data indicate that Su i facilitates the incorporation of newly synthesized subunits into ATP synthase complexes.
Wagner
Karina
K
Institut für Biochemie und Molekularbiologie, Freiburg, Germany.
Perschil
Inge
I
Fichter
Christiane D
CD
van der Laan
Martin
M
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D004586:Electrophoresis / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D017353:Gene Deletion; D015151:Immunoblotting; D008928:Mitochondria / Q000378:metabolism*; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D055503:Protein Multimerization; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
C503883:ATP20 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C116839:ATP18 protein, S cerevisiae; D000251:Adenosine Triphosphatases; D025261:Mitochondrial Proton-Translocating ATPases; C102550:TIM11 protein, S cerevisiae; D006180:Proton-Translocating ATPases; C551523:ATP19 protein, S cerevisiae