Differential cytokine expression in Chlamydophila psittaci genotype A-, B- or D-infected chicken macrophages after exposure to Escherichia coli O2:K1 LPS.
Chlamydophila (Cp.) psittaci and avian pathogenic Escherichia (E.) coli infections contribute to the respiratory disease complex observed in turkeys. Secondary infection with E. coli exacerbates Cp. psittaci pathogenicity and augments E. coli excretion. The innate immune response initiated by both pathogens in their avian host is unknown. We therefore determined the cytokine responses following Cp. psittaci infection and E. coli superinfection of avian monocytes/macrophages by examining gene transcripts of IL-1beta, IL-6, CXCLi2 (IL-8), CXCLi1 (K60), IL-10, IL-12alpha/beta, IL-18, TGF-beta4 and CCLi2 at 4h post-inoculation with different Cp. psittaci strains or 4h post-treatment with avian E. coli LPS of Cp. psittaci pre-infected HD11 cells. Cp. psittaci strains used were 84/55 and 92/1293 (highly virulent), CP3 (low virulent) and 84/2334 (phylogenetically intermediate between Cp. psittaci and Chlamydophila abortus). At 4h post chlamydial infection, an increased expression of IL-1beta and IL-6 as well as CXCLi2, CXCLi1 and CCLi2 was observed compared to levels in uninfected HD11 controls. This effect was less pronounced for the milder CP3 strain. The pro-inflammatory response of Cp. psittaci infected cells to E. coli LPS was significantly lowered compared to uninfected controls, especially when the cells were pre-infected with highly virulent Cp. psittaci strains. In both experiments, exceptionally high IL-10 and no TGF-beta4 responses were observed, and we propose that this could induce macrophage deactivation and NF-kappaB suppression. Consequently, pro-inflammatory and Th1-promoting responses to both the primary Cp. psittaci infection and E. coli would be inhibited, thus explaining the observed aggravated in vivo pathology.
Beeckman
Delphine Sylvie Anne
DS
Department of Molecular Biotechnology, Ghent University, Faculty of Bioscience Engineering, Coupure Links 653, B-9000 Ghent, Belgium. Delphine.Beeckman@UGent.be
Rothwell
Lisa
L
Kaiser
Pete
P
Vanrompay
Daisy C G
DC
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002645:Chickens; D002691:Chlamydophila psittaci / Q000276:immunology* / Q000472:pathogenicity; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000276:immunology*; D020869:Gene Expression Profiling; D005838:Genotype; D006825:Hybridomas; D007113:Immunity, Innate / Q000235:genetics; D018836:Inflammation Mediators / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D009956:Psittacosis / Q000235:genetics / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D013045:Species Specificity; D014774:Virulence
D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides