title: Reversible Ponceau staining as a loading control alternative to actin in Western blots.
abstract: It is becoming standard practice to measure a housekeeping gene, typically actin, in Western blots, as it is the rule in RNA blots. We have applied reversible Ponceau staining to check equal loading of gels and measured actin in parallel under different conditions. Our results show that densitometric analysis is comparable with both techniques. Therefore, routine quantitation of Ponceau staining before antibody probing is validated as an alternative to actin blotting.
authors.affiliation: Department of Biochemistry, School of Pharmacy, University of Granada, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Campus de Cartuja s/n, 18071 Granada, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Romero-Calvo
authors.forename: Borja
authors.initials: B
authors.lastname: Ocón
authors.forename: Patricia
authors.initials: P
authors.lastname: Martínez-Moya
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Suárez
authors.forename: Antonio
authors.initials: A
authors.lastname: Zarzuelo
authors.forename: Olga
authors.initials: O
authors.lastname: Martínez-Augustin
authors.forename: Fermín Sánchez
authors.initials: FS
authors.lastname: de Medina
mesh_terms: D000199:Actins / Q000032:analysis*; D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D002460:Cell Line; D003106:Colon / Q000737:chemistry; D006801:Humans; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D051379:Mice; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000199:Actins