The immunological properties of stroma-free polyhemolysate containing catalase and superoxide dismutase activities prepared by polymerized bovine stroma-free hemolysate.
Crosslinking of ultrapure hemoglobin, crystalline catalase, and superoxide dismutase resulted in a soluble nanodimensional complex of polyhemoglobin-catalase-superoxide dismutase. A less expensive and more convenient way is to crosslink bovine stroma-free hemolysate (stroma-free hemolysate) that already contains hemoglobin, catalase, and superoxide dismutase into polyhemoglobin with catalase and superoxide dismutase activities (stroma-free polyhemolysate) [21]. The objective of the present study is to evaluate the immunological properties of this stroma-free polyhemolysate. Each of three groups of rats received weekly subcutaneous injections of one of the stroma-free polyhemolysate, stroma-free hemolysate, and saline for four weeks. One week after the four cycles of weekly immunization, serum and plasma were collected for C3a complement activation tests and Ouchterlony antibody-antigen precipitation tests, respectively. Results show that stroma-free polyhemolysate retained significant antioxidant enzyme activity. The C3a complement activation test and Ouchterlony test show that four weekly subcutaneous injections of bovine stroma-free polyhemolysate did not result in any immunological reaction in rats when tested this way.
Zhu
Hongli
H
National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University, Xi'an, Shaanxi, China.
Du
Qianqian
Q
Chen
Chao
C
Chang
Thomas Ming Swi
TM
D000818:Animals; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D000975:Antioxidants / Q000008:administration & dosage* / Q000302:isolation & purification / Q000378:metabolism; D001802:Blood Substitutes / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism*; D002374:Catalase / Q000008:administration & dosage* / Q000302:isolation & purification / Q000378:metabolism; D002417:Cattle; D003167:Complement Activation / Q000187:drug effects*; D003176:Complement C3 / Q000378:metabolism; D006454:Hemoglobins / Q000008:administration & dosage* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007109:Immunity / Q000187:drug effects*; D007114:Immunization; D007279:Injections, Subcutaneous; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000008:administration & dosage* / Q000302:isolation & purification / Q000378:metabolism
D000975:Antioxidants; D001802:Blood Substitutes; D003176:Complement C3; D006454:Hemoglobins; C016484:hemoglobin, stroma free; D002374:Catalase; D013482:Superoxide Dismutase; C086134:polyhemoglobin-superoxide dismutase-catalase