title: Cloning of aprE86-1 gene encoding 27 kDa mature fibrinolytic enzyme from Bacillus amyloliquefaciens CH86-1.
abstract: A gene, encoding the major secreted fibrinolytic protein of Bacillus amyloliquefaciens CH86-1, was cloned from the genomic DNA. DNA sequencing showed that the gene, aprE86-1, could direct the synthesis of a mature protein of 275 amino acids long after processing. When aprE86-1 was introduced into B. subtilis, 27 kDa mature protein was produced as expected. The fibrinolytic activity of B. subtilis transformant (TF) was higher than that of B. amyloliquefaciens CH86-1, showing the possibility of increasing fibrinolytic activities of Bacillus strains through genetic engineering.
authors.affiliation: Division of Applied Life Science (BK21) Graduate School, Gyeongsang National University Jinju 660-701, Korea.
authors.forename: Ae Ran
authors.initials: AR
authors.lastname: Lee
authors.forename: Gyoung Min
authors.initials: GM
authors.lastname: Kim
authors.forename: Gun-Hee
authors.initials: GH
authors.lastname: Kwon
authors.forename: Kang Wook
authors.initials: KW
authors.lastname: Lee
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Park
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Chun
authors.forename: Jaeho
authors.initials: J
authors.lastname: Cha
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Song
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D005337:Fibrin / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D005337:Fibrin