New light on NO bonding in Fe(III) heme proteins from resonance Raman spectroscopy and DFT modeling.
Visible and ultraviolet resonance Raman (RR) spectra are reported for Fe(III)(NO) adducts of myoglobin variants with altered polarity in the distal heme pockets. The stretching frequencies of the Fe(III)-NO and N-O bonds, nu(FeN) and nu(NO), are negatively correlated, consistent with backbonding. However, the correlation shifts to lower nu(NO) for variants lacking a distal histidine. DFT modeling reproduces the shifted correlations and shows the shift to be associated with the loss of a lone-pair donor interaction from the distal histidine that selectively strengthens the N-O bond. However, when the model contains strongly electron-withdrawing substituents at the heme beta-positions, nu(FeN) and nu(NO) become positively correlated. This effect results from Fe(III)-N-O bending, which is induced by lone-pair donation to the N(NO) atom. Other mechanisms for bending are discussed, which likewise lead to a positive nu(FeN)/nu(NO) correlation, including thiolate ligation in heme proteins and electron-donating meso-substituents in heme models. The nu(FeN)/nu(NO) data for the Fe(III) complexes are reporters of heme pocket polarity and the accessibility of lone pair, Lewis base donors. Implications for biologically important processes, including NO binding, reductive nitrosylation, and NO reduction, are discussed.
Soldatova
Alexandra V
AV
Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195, USA.
Ibrahim
Mohammed
M
Olson
John S
JS
Czernuszewicz
Roman S
RS
Spiro
Thomas G
TG
D003198:Computer Simulation; D005290:Ferric Compounds / Q000737:chemistry*; D008956:Models, Chemical; D015394:Molecular Structure; D009211:Myoglobin / Q000737:chemistry*; D009569:Nitric Oxide / Q000737:chemistry*; D011789:Quantum Theory; D013059:Spectrum Analysis, Raman
D005290:Ferric Compounds; D009211:Myoglobin; D009569:Nitric Oxide