title: Thermal-assisted refolding: dilution folding initiated at high temperature.
abstract: Protein refolding from unfolded state is usually carried out at low temperature to reduce protein aggregation and proteolytic degradation. This review briefly introduces a unique method for the protein refolding via high temperature, typically at above melting temperature of the protein.
authors.affiliation: Institute of Applied Physics, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan. shiraki@bk.tsukuba.ac.jp
authors.forename: Kentaro
authors.initials: K
authors.lastname: Shiraki
authors.forename: Soichiro
authors.initials: S
authors.lastname: Kayano
mesh_terms: D002479:Inclusion Bodies / Q000737:chemistry*; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry*; D013696:Temperature
chemical_list: D011994:Recombinant Proteins; D012997:Solvents