Distribution of aminogenic activity among potential autochthonous starter cultures for dry fermented sausages.
Any bacterial strain to be used as starter culture should have suitable characteristics, including a lack of amino acid decarboxylase activity. In this study, the decarboxylase activity of 76 bacterial strains, including lactic acid bacteria and gram-positive, catalase-positive cocci, was investigated. These strains were previously isolated from European traditional fermented sausages to develop autochthonous starter cultures. Of all the strains tested, 48% of the lactic acid bacteria strains and 13% of gram-positive, catalase-positive cocci decarboxylated one or more amino acids. Aminogenic potential was strain dependent, although some species had a higher proportion of aminogenic strains than did others. Thus, all Lactobacillus curvatus strains and 70% of Lactobacillus brevis strains had the capacity to produce tyramine and beta-phenylethylamine. Some strains also produced other aromatic amines, such as tryptamine and the diamines putrescine and cadaverine. All the enterococcal strains tested were decarboxylase positive, producing high amounts of tyramine and considerable amounts of beta-phenylethylamine. None of the staphylococcal strains had tyrosine-decarboxylase activity, but some produced other amines. From the aminogenic point of view, Lactobacillus plantarum, Lactobacillus sakei, and Staphylococcus xylosus strains would be the most suitable for use as autochthonous starter cultures for traditional fermented sausages.
Latorre-Moratalla
M L
ML
Departament de Nutrició i Bromatologia, Facultat de Farmàcia, Universitat de Barcelona, E-08028 Barcelona, Spain.
Bover-Cid
S
S
Talon
R
R
Aymerich
T
T
Garriga
M
M
Zanardi
E
E
Ianieri
A
A
Fraqueza
M J
MJ
Elias
M
M
Drosinos
E H
EH
Lauková
A
A
Vidal-Carou
M C
MC
D000596:Amino Acids / Q000032:analysis / Q000096:biosynthesis; D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism*; D001679:Biogenic Amines / Q000032:analysis / Q000096:biosynthesis; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004755:Enterobacteriaceae / Q000201:enzymology / Q000378:metabolism; D005285:Fermentation; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D006801:Humans; D007778:Lactobacillus / Q000201:enzymology / Q000378:metabolism; D008461:Meat Products / Q000382:microbiology*; D013210:Staphylococcus / Q000201:enzymology / Q000378:metabolism; D013552:Swine; D014439:Tyramine / Q000032:analysis / Q000096:biosynthesis
D000596:Amino Acids; D001679:Biogenic Amines; D001142:Aromatic-L-Amino-Acid Decarboxylases; D014439:Tyramine