title: Osteocalcin gene polymorphisms influence concentration of serum osteocalcin and enhance fracture identification.
abstract: Osteoporosis is a major health problem affecting more than 75 million people throughout Europe, the United States, and Japan. Epidemiologic studies have determined that both genetic and environmental factors contribute to the pathogenesis of osteoporosis. We have investigated the association between polymorphisms at the osteocalcin locus and variables linked to bone health. Osteocalcin provides a link between bone and energy metabolism, hence its potential importance as an osteoporosis candidate gene. In this study, we included a total of 996 women (all aged 75 years) from the Osteoporosis Prospective Risk Assessment (OPRA) cohort. We sequenced the osteocalcin gene along with flanking regions to search for novel coding polymorphisms. We also analyzed four polymorphisms selected from within and flanking regions of the osteocalcin gene to study their association with serum total osteocalcin levels (S-TotalOC), total-body (TB) bone mineral density (BMD), fracture, TB fat mass, and body mass index (BMI). The promoter polymorphism rs1800247 was significantly associated with S-TotalOC (p = .012) after controlling for BMI and TB BMD. The polymorphism rs1543297 was significantly associated with prospectively occurring fractures (p = .008). In a model taking into account rs1543297 and rs1800247, along with TB BMD, BMI, smoking, and S-TotalOC, the polymorphisms together were able to identify an additional 6% of women who sustained a fracture (p = .02). We found no association between the polymorphisms and TB BMD, BMI, or TB fat mass. In conclusion, polymorphisms in and around the osteocalcin locus are significantly associated with S-TotalOC and fracture. Genotyping at the osteocalcin locus could add valuable information in the identification of women at risk of osteoporosis.
authors.affiliation: Department of Clinical Sciences, Lund University, Department of Orthopedics, Malmö University Hospital, Malmö, Sweden.
authors.forename: Fiona
authors.initials: F
authors.lastname: McGuigan
authors.forename: Jitender
authors.initials: J
authors.lastname: Kumar
authors.forename: Kaisa K
authors.initials: KK
authors.lastname: Ivaska
authors.forename: Karl J
authors.initials: KJ
authors.lastname: Obrant
authors.forename: Paul
authors.initials: P
authors.lastname: Gerdhem
authors.forename: Kristina
authors.initials: K
authors.lastname: Akesson
mesh_terms: D000368:Aged; D015331:Cohort Studies; D005260:Female; D050723:Fractures, Bone / Q000097:blood* / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D015675:Osteocalcin / Q000097:blood* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D013548:Sweden
chemical_list: D015675:Osteocalcin