Distinct alterations in colonic morphology and physiology in two rat models of enhanced stress-induced anxiety and depression-like behaviour.
Stress and anxiety are important causal and exacerbating factors in functional gastro-intestinal (GI) disorders such as irritable bowel syndrome. Stress affects GI motility, faecal transit and visceral pain sensitivity. Additionally, permeability and function of the gut epithelium, which acts as a barrier between the external environment and the body's internal milieu is altered by stress. However, the effects of an enhanced stress response on colonic morphology require further investigation. We have used two animal models of stress and anxiety, the maternally separated (MS) and Wistar Kyoto (WKY) rats to examine colonic morphology. These rats exhibit increased anxiety behaviours, visceral hypersensitivity and increased stress-induced defecation in the open field arena. At a morphological level, increased mucus secretion and an associated elevation in the number of mucosal goblet cells was observed in the high anxiety rats. Additionally, the mucosal layer was flattened in MS and WKY rats, a finding indicative of mild mucosal damage. Furthermore, the muscular layer of the distal colon in these animals was thickened, an observation that may have implications for faecal transit and visceral pain perception. This study provides evidence of altered colonic function and morphology in two animal models with a heightened response to stress.
O'Malley
Dervla
D
Laboratory of Neurogastroenterology, Alimentary Pharmabiotic Centre, BioSciences Institute, University College Cork, Cork, Ireland.
Julio-Pieper
Marcela
M
Gibney
Sinead M
SM
Dinan
Timothy G
TG
Cryan
John F
JF
D000818:Animals; D001007:Anxiety / Q000503:physiopathology*; D003106:Colon / Q000473:pathology* / Q000502:physiology / Q000503:physiopathology*; D003863:Depression / Q000503:physiopathology*; D005769:Gastrointestinal Motility / Q000502:physiology; D007413:Intestinal Mucosa / Q000502:physiology / Q000503:physiopathology; D043183:Irritable Bowel Syndrome / Q000503:physiopathology; D008426:Maternal Deprivation; D051381:Rats; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000503:physiopathology*