title: HLA-B27 polymorphism in patients with juvenile and adult-onset ankylosing spondylitis in Southern China.
abstract: Distribution of B27 subtypes in juvenile and adult-onset ankylosing spondylitis (JAS and AAS) in Southern China was studied. A total of 505 patients belonged to Han population were included (145 JAS and 360 AAS patients), and 1368 healthy individuals were included as controls. Human leukocyte antigen (HLA)-B27 typing was performed by Luminex liquid array combining polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) and/or serological method. HLA-B27 subtyping was performed by polymerase chain reaction-sequence specific primer (PCR-SSP). The sequence-based typing was performed for the B*2715 samples to verify the PCR-SSP results. HLA-B27 was presented in 453 of 505 patients (89.7%), compared with 74 of 1368 controls (5.41%). B*2704 subtype in AS group was significantly higher than controls and B*2705 subtype significantly lower. B*2715 and B*2702 were found in 1.32% and 0.66% of the B27-positive patients but none in controls, and there was no significant difference between either of them and controls. B27-positive patients were 134 (92.4%) in JAS group and 319 (88.6%) in AAS group. There was no significant difference for B27 subtypes distribution between JAS (B*2704, 05, 15) and AAS (B*2704, 05, 15, 02) groups. The frequency of B*2715 in two groups was 3 (2.24%) and 3 (0.94%), respectively. The onset age of three JAS patients carrying B*2715 was 5, 9 and 13 years old, respectively. Our results suggested that B*2704 was the predominant subtype in AS patients in Southern China. B*2715 was observed in AS group only and slightly more in JAS than in AAS, and the patients carrying this allele tended to have early onset, B*2715 may be disease-association subtype.
authors.affiliation: Department of Rheumatology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Mou
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Gu
authors.forename: Z
authors.initials: Z
authors.lastname: Liao
authors.forename: Z
authors.initials: Z
authors.lastname: Lin
authors.forename: Q
authors.initials: Q
authors.lastname: Wei
authors.forename: J
authors.initials: J
authors.lastname: Huang
authors.forename: Q
authors.initials: Q
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D017668:Age of Onset; D002648:Child; D002681:China; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D015796:HLA-B27 Antigen / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic; D013167:Spondylitis, Ankylosing / Q000453:epidemiology / Q000235:genetics*; D055815:Young Adult
chemical_list: D015796:HLA-B27 Antigen