Effects of hydrostatic pressure, agitation and CO2 stress on Phytophthora nicotianae zoospore survival.
BACKGROUND
Phytophthora nicotianae Breda de Haan is a common pathogen of ornamental plants in recycled irrigation systems. In a previous study, annual vinca (Catharanthus roseus Don) inoculated with zoospore suspensions using a CO(2)-pressurized sprayer had less foliage blight than plants inoculated using a hand sprayer. Here, the impact of hydrostatic pressure, agitation and aeration with CO(2) on the survival of P. nicotianae zoospores was examined.


RESULTS
Exposure of zoospores to 840 kPa hydrostatic pressure for 8 min or agitation at a mixing intensity (G) of 6483 s(-1) for 4 min at 22-23 degrees C did not kill zoospores, but resulted in viable cysts. Motile and forcefully encysted zoospores of P. nicotianae were equally infectious on vinca or lupine (Lupinus polyphylus Lindl.). Bubbling CO(2) into zoospore-infested water at 110.4 mL (0.2 g) min(-1) for 5 min caused 81% reduction in the number of germinated zoospores. Pressure at 630 kPa (16.3 g CO(2)) or 70 kPa (3.85 g CO(2)) facilitated CO(2) injection and shortened the zoospore inactivation time to 30 s. When air was bubbled through the suspension, germination was similar to the control.


CONCLUSIONS
Exposure to CO(2) killed P. nicotianae zoospores in water. Neither pressure nor agitation had an effect on zoospore viability or infectivity. Based on results of this study, the authors designed a recycling CO(2) water treatment system that is currently under evaluation.
Ahonsi
Monday O
MO
Hampton Roads Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University, Virginia Beach, VA 23455, USA. mahonsi@igb.uiuc.edu
Banko
Thomas J
TJ
Doane
Stargel R
SR
Demuren
Ayodeji O
AO
Copes
Warren E
WE
Hong
Chuanxue
C
D000388:Air; D002245:Carbon Dioxide / Q000494:pharmacology*; D006874:Hydrostatic Pressure; D009038:Motion; D010838:Phytophthora / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology*; D013170:Spores / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology*; D013312:Stress, Physiological / Q000187:drug effects*; D016019:Survival Analysis
D002245:Carbon Dioxide