Fabrication of three-dimensional cell constructs using temperature-responsive hydrogel.
A morphologically controlled three-dimensional (3D) cell construct composed of only cells and having no scaffold material might be a valuable biologic material for tissue engineering applications, as the scaffold materials can cause delay of tissue regeneration in some conditions. To obtain such a 3D cell construct, a 3D thermoresponsive hydrogel (poly-N-isopropylacrylamide) was prepared as a mold material that changes its volume depending on the temperature. Three-dimensional osteoblast cell constructs with a variety of morphologies as well as a monolayered cell sheet were obtained by decreasing the surrounding temperature of the hydrogel designed with a predefined shape and formed by curing in a polymer mold manufactured via 3D printing. The cell sheet or 3D cell constructs detachment resulted from a simple change in the gel volume, not by the surface chemistry of the gel, because the surface hydrophilicity of the gel was maintained over a wide temperature range. These 2D/3D cell constructs have numbers of exciting applications such as cell carriers for tissue regeneration or as model tissues for the biological study.
Sasaki
Jun-Ichi
J
Department of Oromaxillofacial Regeneration, Osaka University, Suita, Japan.
Asoh
Taka-Aki
TA
Matsumoto
Takuya
T
Egusa
Hiroshi
H
Sohmura
Taiji
T
Alsberg
Eben
E
Akashi
Mitsuru
M
Yatani
Hirofumi
H
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D013499:Surface Properties; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*
D000180:Acrylic Resins; D001672:Biocompatible Materials; D020100:Hydrogels; C052970:poly-N-isopropylacrylamide