Assembly of a large Y-STR haplotype database for the Czech population and investigation of its substructure.
Twelve Y-chromosomal short tandem repeats (Y-STR) (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a, DYS385b, DYS437, DYS438, and DYS439) included in the PowerPlex Y Kit (Promega Corporation, Madison, USA) were studied for 1750 unrelated males living in 14 regions of the Czech Republic. A total of 1148 different haplotypes were found. The overall haplotype diversity (HD) was determined as 0.998. Analysis of Molecular Variance (AMOVA) reveals non-significant distances between regions concerning their haplotype distribution, thus allowing to use the whole sample as a representative reference database of the Czech Republic. Median network analysis shows a remarkable bipartite composition of the Czech haplotypes, falling in distinct clusters with Eastern and Western European roots.
Zastera
Jan
J
Laboratory for Molecular Genetics and Oncology, Genomac International, Ltd., Prague, Czech Republic.
Roewer
Lutz
L
Willuweit
Sascha
S
Sekerka
Patrik
P
Benesova
Lucie
L
Minarik
Marek
M
D041322:Chromosomes, Human, Y; D018153:Czech Republic; D016172:DNA Fingerprinting; D030561:Databases, Nucleic Acid; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences