title: The circadian rhythm of expression of Bmal1 and Cry1 in peripheral blood mononuclear cells of preterm neonates.
abstract: OBJECTIVE
  To determine circadian rhythms of circadian genes in the peripheral blood mononuclear cells in preterm neonates.
  
  
  METHODS
  Ten premature neonates, gestational age from 30 to 31 weeks were recruited. Infants with birth asphyxia, RDS, apnea, malformation, infection or haemolytic diseases were excluded. At 7AM and 7PM on the 1st day, 7th day and 14th day of hospitalisation, peripheral venous blood was obtained from the premature babies and real-time RTPCR was used to determine the expression of Bmal1 and Cry1.
  
  
  RESULTS
  The expression of bmal1 did not have statistical difference between 7AM and 7PM on the 1st day, 7th day and 14th day. Neither did the cry1.
  
  
  CONCLUSIONS
  There was no circadian rhythm of bmal1 and cry1 in PBMC of preterm neonates in our NICU.
authors.affiliation: Neonatal Intensive Care Unit, the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China. chen_an29@sina.com
authors.forename: An
authors.initials: A
authors.lastname: Chen
mesh_terms: D056930:ARNTL Transcription Factors / Q000235:genetics / Q000378:metabolism*; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology*; D056931:Cryptochromes / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D015931:Intensive Care, Neonatal; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D056930:ARNTL Transcription Factors; C538960:BMAL1 protein, human; C538963:CRY1 protein, human; D056931:Cryptochromes; D012333:RNA, Messenger