[Effect of fengshiqing on interleukin-4, gamma-interferon and chemotactic factor in rats with collagen type II].
OBJECTIVE
To study the regulatory effect of fengshiqing (FSQ) on interleukin 4 (IL-4), gamma-interferon (gamma-IFN), macrophage inflammatory protein 1alpha (MIP-1alpha) and collagen type II antibody (C II Ab) in serum and supernatant of synovial cell culture of rat with rheumatoid arthritis (RA), to explore the mechanism of action of clearing-heat and activating blood method for treatment of RA.


METHODS
RA rat model was induced by C II Ab combined with Freund's complete adjuvant, and the levels of IL-4, gamma-IFN, MIP-1alpha and C II Ab in serum and supernatant of synovial cell were detected by ELISA.


RESULTS
As compared with the normal group, serum level of C II Ab in the model group was significantly higher (P < 0.01), serum and supernatant contents of IL-4 on the 14th and 28th day of modeling were lower and those of gamma-IFN and MIP-1alpha were higher, the difference showed statistical significance (P < 0.05 or P < 0.01). After being treated with FSQ and IL-4 contents in serum and supernatant as well as MIP-1alpha in supernatant restored on the 14th day (P < 0.05 or P < 0.01), while all the indexes restored on the 28th day.


CONCLUSIONS
FSQ could evidently up-regulate the level of IL-4 and down-regulate that of MIP-1alpha in serum and local synovial membrane in RA rats, and shows a suppressive trend of gamma-IFN, so as to maintain the Th1/Th2 equilibrium, suppress the cellular and humoral immune response in the local synovial membrane, and alleviate the chronic changes of arthritis, synovitis and vasculitis.
Cao
Wei
W
Department of Rheumatology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing.
Jiang
Quan
Q
Wu
Zhen-yu
ZY
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D054405:Chemokine CCL3 / Q000378:metabolism*; D024043:Collagen Type II / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D058408:Th1-Th2 Balance
D054405:Chemokine CCL3; D024043:Collagen Type II; D004365:Drugs, Chinese Herbal; D015847:Interleukin-4; D007371:Interferon-gamma