C/EBPα down-regulation is associated with reduced hepatic cellular viability during hypoxia in vitro and in vivo.
C/EBPα transcription factor is a key regulator in liver biology and was preliminarily shown to be down-regulated in hypoxic primary rat hepatocytes. The aim of this study was to explore the possible association between C/EBPα expression level and hepatocyte viability in both the in-vitro cultured hypoxic rat primary hepatocytes and two models of acute liver hypoxia induced by carbon tetrachloride or Fas antibody. C/EBPα mRNA was significantly down-regulated under hypoxic conditions both in vitro and in vivo, which was paralleled by a similar decrease in hepatocyte viability and partially reversed by 3D matrix and dexamethasone. These results suggested that C/EBPα down-regulation may be one mechanism of reduced hepatocyte viability in these settings.
Zhou
Qian Yun
QY
Department of Clinical Laboratory, The First Affiliated Hospital, Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing, 400016, PR China.
Liu
Ding
D
Huang
Shi Feng
SF
Wen
Yang An
YA
Luo
Peng
P
Xiang
Yu
Y
Sun
Shan
S
Dong
Yu Fang
YF
Zhang
Li Ping
LP
D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism*; D015687:Cell Hypoxia; D002470:Cell Survival; D015536:Down-Regulation; D022781:Hepatocytes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
D022763:CCAAT-Enhancer-Binding Protein-alpha; D012333:RNA, Messenger