title: Platelet-rich plasma diminishes calvarial bone repair associated with alterations in collagen matrix composition and elevated CD34+ cell prevalence.
abstract: The interaction between platelets and both type I and III collagens plays an important role in modulating platelet adhesion and aggregation, also contributing to the chemotaxis of CD34+ cells. The interaction with type III collagen can maintain high levels of collagen and alter the biology of bone repair when the PRP is used. The aim of this study was to evaluate the effect of platelet-rich plasma (PRP) and autograft on the presence of type III and type I collagens, the ratio between them, as well as the presence of CD34+ progenitor cells, while comparing these results by means of a histomorphometric analysis of the bone tissue. Four bone defects (8.0mm in diameter and 2.0mm in depth) were produced on the calvarium of 23 rabbits. The surgical defects were treated with either autogenous bone grafts, autogenous bone grafts with PRP and PRP alone. Animals were euthanized at 2, 4 or 6 weeks post-surgery. Histological, histomorphometric and immunohistochemical analyses were performed to assess repair time, as well as the expression of type I and III collagens, and number of progenitor CD34+ cells. Data were analyzed using the ANOVA and Student-Newman-Keuls test (alpha=5%). An enlarged granulation and medullary tissue areas in the PRP groups were observed. The use of PRP in this study hindered bone deposition, also enhanced type III to type I collagen ratio and the chemotaxis of CD34+ progenitor cells, similarly to a thrombogenic effect.
authors.affiliation: Masters Program in Clinical Dentistry, Positivo University, Curitiba, Brazil. afgiovanini@gmail.com
authors.forename: Allan Fernando
authors.initials: AF
authors.lastname: Giovanini
authors.forename: Tatiana Miranda
authors.initials: TM
authors.lastname: Deliberador
authors.forename: Carla Castiglia
authors.initials: CC
authors.lastname: Gonzaga
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: de Oliveira Filho
authors.forename: Isabella
authors.initials: I
authors.lastname: Göhringer
authors.forename: Juliane
authors.initials: J
authors.lastname: Kuczera
authors.forename: João Cesar
authors.initials: JC
authors.lastname: Zielak
authors.forename: Cícero
authors.initials: C
authors.lastname: de Andrade Urban
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D016025:Bone Transplantation / Q000502:physiology; D002633:Chemotaxis / Q000502:physiology; D003094:Collagen / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D053657:Platelet-Rich Plasma; D011817:Rabbits; D012886:Skull / Q000293:injuries* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D018952:Antigens, CD34; D024042:Collagen Type I; D024061:Collagen Type III; D003094:Collagen