Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy.
The importance and widespread incidence of Fusarium poae as a natural contaminant of wheat in different climatic areas warrants investigation into the genetic diversity and toxin profile of a northern Italy population. Eighty-one strains of F. poae isolated from durum wheat kernels, identified by species-specific polymerase chain reaction and translation elongation factor-1alpha gene sequence analysis, were genetically characterized by the amplified fragment length polymorphism (AFLP) technique and analysed by high-pressure liquid chromatography for their ability to produce the beauvericin (BEA) and trichothecene mycotoxins. A high level of variability was observed by using AFLP analyses, with the lowest level of genetic similarity among the strains being approximately 61%. Most of the strains, 95%, produced BEA at <2655 microg g(-1); 88% produced the trichothecene nivalenol at <865 microg g(-1) and 76% produced the trichothecene fusarenon-X at <167 microg g(-1). These data show that F. poae can produce high amounts of BEA together with trichothecenes, and can represent a high potential mycotoxin risk in Italy for wheat colonized by this species.
Somma
S
S
Institute of Sciences of Food Production, Research National Council (ISPA-CNR), Bari, Italy.
Alvarez
C
C
Ricci
V
V
Ferracane
L
L
Ritieni
A
A
Logrieco
A
A
Moretti
A
A
D054458:Amplified Fragment Length Polymorphism Analysis; D002851:Chromatography, High Pressure Liquid; D047630:Depsipeptides / Q000096:biosynthesis* / Q000737:chemistry; D002523:Edible Grain / Q000382:microbiology; D005506:Food Contamination; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D005670:Fusarium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D005800:Genes, Fungal; D014644:Genetic Variation; D007558:Italy; D057230:Limit of Detection; D016533:Mycological Typing Techniques; D009183:Mycotoxins / Q000096:biosynthesis* / Q000737:chemistry; D020648:Peptide Elongation Factor 1 / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D053719:Tandem Mass Spectrometry; D035602:Transcriptional Elongation Factors / Q000737:chemistry / Q000235:genetics; D014255:Trichothecenes / Q000096:biosynthesis* / Q000737:chemistry; D014908:Triticum / Q000382:microbiology*
D047630:Depsipeptides; D005656:Fungal Proteins; D009183:Mycotoxins; D020648:Peptide Elongation Factor 1; D035602:Transcriptional Elongation Factors; D014255:Trichothecenes; C004456:beauvericin