title: [Expression of the starfish complement component C3 gene homologue under the influence of bacterial lipopolysaccharide].
abstract: The fragment of a homologue of complement component C3 gene has been cloned and sequenced from the starfish, Asterias rubens. Phylogenetic analysis of ArC3-like gene demonstrates that ArC3-like gene has close similarity to C3 gene homologues of Deuterostomia invertebrate animals. High level of ArC3-like gene expression was identified in circulating cells (coelomocytes), in a gut's derivate (hepatopancreas) and in male gonada but not in stomach, female gonad and rectal gland of A. rubens starfish. ArC3-like gene expression was shown in all types of starfish coelomocytes: in lymphocyte-like cells, granular and nongranular amebocytes. Injection of bacterial lipopolysaccharide (LPS) solution into the coelomic cavity of starfish leads to the increase of ArC3-like gene expression in coelomocytes and hepatopancreas over the control level of sterile sea water injection. The level of ArC3-like gene expression increased in response to LPS reaching the maximum 6 h after the stimulation, and decreased to basal level 24 h after the stimulation. Injection of LPS solution stimulated the increase of ArC3-like gene expression level in hepatopancreas reaching the maximum 6-12 h after the stimulation, and the level of mRNA of ArC3-like gene had still been increased 48 h after LPS injection. The data demonstrates sustained positive regulation of ArC3-like gene expression under the influence of LPS.
authors.forename: D A
authors.initials: DA
authors.lastname: Mogilenko
authors.forename: I V
authors.initials: IV
authors.lastname: Kudriavtsev
authors.forename: S V
authors.initials: SV
authors.lastname: Orlov
authors.forename: A D
authors.initials: AD
authors.lastname: Kharazova
authors.forename: A V
authors.initials: AV
authors.lastname: Polevshchikov
mesh_terms: D000818:Animals; D047271:Asterias / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D003176:Complement C3 / Q000145:classification / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D010802:Phylogeny
chemical_list: D003176:Complement C3; D018076:DNA, Complementary; D008070:Lipopolysaccharides