title: An efficient approach to the production of vaccines against the malaria parasite.
abstract: In malaria vaccine research, one of the major obstacles has been the difficulty of expressing recombinant malarial proteins and it is mainly due to the lack of an efficient methodology for the synthesis of sufficient quantity of quality proteins. We demonstrate that the wheat germ cell-free protein synthesis system can be applied for the successful production of leading malaria vaccine candidate antigens and, thus, prove that it may be a key tool for malaria vaccine research.
authors.affiliation: Cell-Free Science and Technology Research Center and Venture Business Laboratory, Ehime University, Ehime, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Tsuboi
authors.forename: Satoru
authors.initials: S
authors.lastname: Takeo
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sawasaki
authors.forename: Motomi
authors.initials: M
authors.lastname: Torii
authors.forename: Yaeta
authors.initials: Y
authors.lastname: Endo
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D002474:Cell-Free System; D002846:Chromatography, Affinity; D005260:Female; D006031:Glycosylation; D006801:Humans; D017780:Malaria Vaccines / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology*; D014176:Protein Biosynthesis; D015202:Protein Engineering / Q000379:methods*; D017510:Protein Folding; D011499:Protein Processing, Post-Translational; D015800:Protozoan Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D012639:Seeds / Q000378:metabolism; D014158:Transcription, Genetic; D014908:Triticum / Q000196:embryology / Q000235:genetics / Q000378:metabolism*
chemical_list: D000953:Antigens, Protozoan; D017780:Malaria Vaccines; C071284:Pfs25 protein, Plasmodium falciparum; D015800:Protozoan Proteins