[Effects of surfactin on proliferation, apoptosis and cytoskeleton in human breast cancer MCF-7 cells].
We studied the effect of surfactin on cell proliferation, apoptosis and the cytoskeleton in human breast cancer cell line MCF-7 in vitro. The result of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) showed that the surfactin inhibited proliferation of MCF-7 cells in a dose- and time-dependent manner, with IC50 at 48 h of 27.3 micromol/L. Surfactin-induced cell death was considered to be apoptotic by observing the typical apoptotic morphological changes by AO/EB staining. Flow cytometric analysis also demonstrated that surfactin caused time-dependent apoptosis of MCF-7 cells through cell arrest at G2/M phase. Immunofluorescence and Western blotting showed that surfactin significantly suppressed the expression of vimentin, induced the alpha-tubulin depolymerization and rearrangement and then the skeleton system of the cells changed dramatically. Based on our findings, surfactin can significantly inhibit the growth of MCF-7 cells and induce apoptosis.
Cao
Xiaohong
X
College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. wahwawayu@126.com
Wang
Aihua
A
Wang
Chunling
C
Lu
Meifang
M
Jiao
Runzhi
R
Zhu
Hui
H
Zhao
Sisi
S
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects*; D005260:Female; D006801:Humans; D055666:Lipopeptides / Q000494:pharmacology*; D010456:Peptides, Cyclic / Q000494:pharmacology*
D000970:Antineoplastic Agents; D055666:Lipopeptides; D010456:Peptides, Cyclic; C009365:surfactin peptide