[Evaluation of oral injuries in experimental induced diabetes mellitus by analysis of some gingival fluid markers].
OBJECTIVE
The objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (AST) and IL1-beta in gingival crevicular fluid (GCF) on animal model with experimentally induced diabetes mellitus and periodontal disease.


METHODS
During our study we used 15 Wistar rats, divided into three groups: I--control, II--with experimental model of periodontal disease, III--with experimental model of periodontal disease and diabetes. The sampling of GCF was realized using Whatman no. 1 paper strips introduced in the gingival sulci from mandibular left and right molars. For the determination of AST in GCF we used a spectrophotometric method while gingival fluid IL-1beta determinations were realized through immune enzymatic methods, using an ELISA kit (rlL-1beta).


RESULTS
The results displayed 3.47 times increased gingival fluid AST values in the stimulated experimental model (with periodontal alteration) when compared to control values (without periodontal disease), while in diabetes an increase of 6.139 times higher compared to control (without periodontal disease) were recorded. Moreover, the levels of periodontal disorder-induced IL-1beta were 3.54 times higher compared to control (group II--218.490 pg/mL vs group I--61.691 pg/mL), the most significant increase being recorded for the group with diabetes associated to periodontitis (492.129 pg/mL).


CONCLUSIONS
The present study found a high level of agreement between the presence of AST and IL-1beta in GCF in the experimental model of diabetes associated to periodontal disease, elevated when compared with the periodontal disease only model, and both higher when compared to control group.
Foia
Liliana
L
Departamentul de Biochimie, Facultatea de Medicină Dentară, Universitatea de Medicini Si Farmacie Gr.T. Popa Iaşi.
Toma
Vasilica
V
Ungureanu
Didona
D
Zlei
Mihaela
M
Indrei
Anca
A
Forna
Doriana
D
Habas
Danisia
D
Nanescu
Sonia
S
D000465:Algorithms; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000201:enzymology / Q000276:immunology / Q000628:therapy; D004195:Disease Models, Animal; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005883:Gingival Crevicular Fluid / Q000737:chemistry* / Q000201:enzymology / Q000276:immunology; D053583:Interleukin-1beta / Q000032:analysis; D010512:Periodontal Index; D010518:Periodontitis / Q000201:enzymology / Q000209:etiology* / Q000276:immunology / Q000628:therapy; D051381:Rats; D017208:Rats, Wistar; D013053:Spectrophotometry
D015415:Biomarkers; D053583:Interleukin-1beta; D001219:Aspartate Aminotransferases