title: Atmospheric pressure femtosecond laser imaging mass spectrometry.
abstract: A novel atmospheric pressure imaging mass spectrometry approach that offers improved lateral resolution (10 microm) using near-infrared femtosecond laser pulses for nonresonant desorption and ionization of sample constituents without the need of a laser-absorbing matrix is demonstrated. As a proof of concept the method was used to image a two-chemical pattern in paper. To demonstrate the ability of the approach to analyze biological tissue, a monolayer of onion epidermis was imaged allowing the chemical visualization of individual cells using mass spectrometry at ambient conditions for the first time. As the spatial resolution is currently limited by the limit of detection of the setup (approximately 500 fmol limit of detection for citric acid), improvements in sensitivity will increase the achievable spatial resolution.
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA.
authors.forename: Yves
authors.initials: Y
authors.lastname: Coello
authors.forename: A Daniel
authors.initials: AD
authors.lastname: Jones
authors.forename: Tissa C
authors.initials: TC
authors.lastname: Gunaratne
authors.forename: Marcos
authors.initials: M
authors.lastname: Dantus
mesh_terms: D001274:Atmospheric Pressure; D019343:Citric Acid / Q000737:chemistry; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D019697:Onions / Q000737:chemistry; D013997:Time Factors
chemical_list: D019343:Citric Acid