title: Expression of beta-1,4-galactosyltransferase-I affects cellular adhesion in human peripheral blood CD4+ T cells.
abstract: beta-1,4-galactosyltransferase-I (beta-1,4-GalT-I) has two isoforms that differ only in the length of their cytoplasmic domains. In this study, we found that both the long and short isoforms of beta-1,4-GalT-I were expressed in human CD4(+) T lymphocytes, and localized in the cytoplasm and on the plasma membrane. The expression level of beta-1,4-GalT-I was increased in CD4(+) T cells after stimulation with interleukin (IL)-2, and was further increased after stimulation with IL-2+IL-12, but decreased after stimulation with IL-2+IL-4 when compared to stimulation with IL-2 alone. We also demonstrated that the cellular adhesion of CD4(+) T cells was significantly increased upon cytokine stimulation, and was inhibited by alpha-lactalbumin, indicating that the increase in adhesion was positively correlated with the expression and activity of long beta-1,4-GalT-I. Collectively, the data suggest that beta-1,4-GalT-I plays a role in the cellular adhesion of CD4(+) T cells.
authors.affiliation: Department of Microbiology and Immunology, Medical School of Nantong University, 19 Qixiu Road, Nantong, Jiangsu 226001, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Han
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Zhou
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Ji
authors.forename: Aiguo
authors.initials: A
authors.lastname: Shen
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Sun
authors.forename: Yingqing
authors.initials: Y
authors.lastname: Hu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000201:enzymology* / Q000276:immunology; D002448:Cell Adhesion; D002478:Cells, Cultured; D005700:Galactosyltransferases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D018664:Interleukin-12 / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D007527:Isoenzymes / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D007376:Interleukin-2; D007527:Isoenzymes; D012333:RNA, Messenger; D018664:Interleukin-12; D015847:Interleukin-4; D005700:Galactosyltransferases; C119701:beta-1,4-galactosyltransferase I