Effect of NlpE overproduction on multidrug resistance in Escherichia coli.
NlpE, an outer membrane lipoprotein, functions during envelope stress responses in Gram-negative bacteria. In this study, we report that overproduction of NlpE increases multidrug and copper resistance through activation of the genes encoding the AcrD and MdtABC multidrug efflux pumps in Escherichia coli.
Nishino
Kunihiko
K
Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan. nishino@sanken.osaka-u.ac.jp
Yamasaki
Seiji
S
Hayashi-Nishino
Mitsuko
M
Yamaguchi
Akihito
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D003300:Copper / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D008074:Lipoproteins / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics; D008826:Microbial Sensitivity Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; D026901:Membrane Transport Proteins; C095021:NlpE protein, E coli; D003300:Copper