Molecular cytogenetic analysis of Korean patients with Waldenström macroglobulinemia.
To compare the molecular cytogenetic characteristics between Waldenström macroglobulinemia (WM) and multiple myeloma (MM), we performed interphase fluorescent in situ hybridization (FISH) in Korean patients with WM and MM. Forty patients with WM and 132 patients with MM were enrolled onto the study. FISH was performed with seven different probes: 6q21, 6q23, CEP4, CEP9, immunoglobulin (IgH) breakapart, RB1 gene, and 1q25. Out of 22 WM patients, 4 (18%) had abnormal karyotypes, mainly structural changes on conventional karyotyping. After performing FISH for the available 29 cases, deletions of 6q23 and 6q21 were newly detected in 3 cases (10%). There was no other anomaly, including trisomy 4 in WM. No 6q deletion was observed in MM patients, but RB1 deletion was the most common change (45%), followed by IgH translocation (42%) and gain of 1q (38%). In conclusion, Korean WM patients had a low rate of 6q deletion (10%) and no trisomy 4.
Bang
Soo-Mee
SM
Seoul National University Bundang Hospital, 300 Gumi-dong, Bundang-gu, Seongnam-si, Gyeongi-do, 463-707, Korea.
Seo
Ji-Weon
JW
Park
Kyung Un
KU
Kim
Seok Jin
SJ
Kim
Kihyun
K
Kim
Sun-Hee
SH
Cho
Sung Ran
SR
Kim
Hugh C
HC
Song
Jaewoo
J
Kim
Jin Seok
JS
Kim
Kyung Hee
KH
Lee
Jae Hoon
JH
Lee
Je-Jung
JJ
Shin
Myung Geun
MG
Suh
Cheolwon
C
Chi
Hyun Sook
HS
Oh
Do-Yeun
DY
Won
Jong-Ho
JH
Kim
Hyo Jung
HJ
Yoon
Sung-Soo
SS
Lee
Dong Soon
DS
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000473:pathology; D002869:Chromosome Aberrations; D002896:Chromosomes, Human, Pair 6; D015331:Cohort Studies; D005260:Female; D017353:Gene Deletion; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping; D007723:Korea; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics; D008258:Waldenstrom Macroglobulinemia / Q000235:genetics* / Q000473:pathology
D007143:Immunoglobulin Heavy Chains