Resident peritoneal inflammatory cells are pivotal in the development of experimental atherosclerosis.
OBJECTIVE
Based on evidence that ionizing radiation can ameliorate chronic and autoimmune diseases in patients and experimental animals, we investigated the effects of radiation on the induction and development of experimental atherogenesis.


METHODS
Male New Zealand rabbits were divided into 5 groups and given an atherogenic diet for 90 days. Peritoneal and thoracic areas (9 Gy) were irradiated on the 1st and 45th days for groups 1 and 2, the 45th day for groups 3 and 4, and not at all for group 5. Prior to irradiation, the peritoneal cavity of animals from groups 1 and 3 was washed with buffered saline. Cells collected by peritoneal washing were reinfused into the peritoneal cavity of the same animal after irradiation. Animals from groups 2 and 4 were intraperitoneally injected with saline as a control.


RESULTS
Despite similar lipid profiles among the experimental groups, the percentage of aortas covered by plaques was remarkably reduced (p<0.001) among animals submitted to irradiation (groups 2 and 4). These differences were completely abolished in irradiated animals reconstituted with their own peritoneal cells.


CONCLUSIONS
These findings point to an important role of resident inflammatory peritoneal cells in experimental atherogenesis.
Relvas
Waldir G M
WG
Department of Medicine, Federal University of Sao Paulo, Sao Paulo, Brazil.
Izar
Maria C O
MC
Segreto
Helena R C
HR
Giordani
Adelmo J
AJ
Ihara
Silvia S M
SS
Mariano
Mario
M
Lopes
Jose D
JD
Popi
Ana F
AF
Helfenstein
Tatiana
T
Pomaro
Daniel
D
Póvoa
Rui M S
RM
Carvalho
Antonio C C
AC
Fonseca
Francisco A H
FA
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology*; D050197:Atherosclerosis / Q000209:etiology*; D005434:Flow Cytometry; D007249:Inflammation / Q000209:etiology*; D017737:Macrophages, Peritoneal / Q000502:physiology*; D008297:Male; D009000:Monocytes / Q000502:physiology*; D010529:Peritoneal Cavity / Q000166:cytology* / Q000528:radiation effects; D010533:Peritoneal Lavage; D035422:Pleural Cavity / Q000166:cytology / Q000528:radiation effects; D011817:Rabbits; D011839:Radiation, Ionizing