title: [Association between insulin-like growth factor-1 receptor gene polymorphisms and with susceptibility to adolescent idiopathic scoliosis].
abstract: OBJECTIVE
  To investigate the association of insulin-like growth factor-1 receptor (IGF-1R) gene polymorphisms and with susceptibility to adolescent idiopathic scoliosis (AIS).
  
  
  METHODS
  This study included 200 patients with AIS and 200 healthy controls. Height, menarche status, curve pattern, Cobb angle, and Risser sign in female patients were recorded. Blood samples were taken form each subject by venipuncture. Genetic DNA was extract from peripheral blood leukocytes using standard phenol/chloroform extraction. PCR-RFLP (polymerase chain reaction-restriction-fragment length polymorphism) was used for the genotyping.
  
  
  RESULTS
  The genotype and allele frequency distribution were similar between AIS and normal control (P>0.05). The mean maximum Cobb angle, Risser sign, menarche status of different genotypes of IGF-1R gene were similar with each other among female AIS patients (P>0.05).
  
  
  CONCLUSIONS
  The IGF-1R gene is neither associated with the occurrence nor the curve severity of AIS.
authors.affiliation: Department of Spine Surgery, the Affiliated Drum Tower Hospital of Medical School, Nanjing University, Nanjing 210008, China.
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Qiu
authors.forename: Zhi-jun
authors.initials: ZJ
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Shu
authors.forename: Xin-hua
authors.initials: XH
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D017526:Receptor, IGF Type 1 / Q000235:genetics*; D012600:Scoliosis / Q000235:genetics*; D055815:Young Adult
chemical_list: D017526:Receptor, IGF Type 1