Interferon-gamma inhibits metalloproteinase activity and cytotrophoblast cell migration.
OBJECTIVE
Establishment of a successful pregnancy relies on a complex fetal-mother communication that starts with the embryo adhering and invading the endometrium. This requires remodeling of extracellular matrix, performed by metalloproteinases. Cytokines, such as interferon-gamma (IFN-gamma), play a role in implantation and could affect the success of pregnancy.


METHODS
Using JEG-3 cell line as model, we cultured the cells in the presence or absence of IFN-gamma and determined the activities of MMP-2 and MMP-9 using zymography and the secretion of leptin using Western blot.


RESULTS
Interferon-gamma inhibits gelatinase activity from MMP-2 and MMP-9 in a dose-dependent manner, reducing the secretion of leptin (not because of a general inhibition on protein synthesis) and impairs cell migration on Matrigel.


CONCLUSIONS
Our results correlate with previous reports from our laboratory indicating that IFN- gamma is deleterious for mouse embryo outgrowth, having an effect on metalloproteinases activity as well as leptin secretion.
Fontana
Vanina Andrea
VA
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Sanchez
Melisa
M
Cebral
Elisa
E
Calvo
Juan Carlos
JC
D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D020738:Leptin / Q000096:biosynthesis*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D014327:Trophoblasts / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology
D020738:Leptin; D007371:Interferon-gamma; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9