Spliceosomal snRNA modifications and their function.
Spliceosomal snRNAs are extensively 2'-O-methylated and pseudouridylated. The modified nucleotides are relatively highly conserved across species, and are often clustered in regions of functional importance in pre-mRNA splicing. Over the past decade, the study of the mechanisms and functions of spliceosomal snRNA modifications has intensified. Two independent mechanisms behind these modifications, RNA-independent (protein-only) and RNA-dependent (RNA-guided), have been discovered. The role of spliceosomal snRNA modifications in snRNP biogenesis and spliceosome assembly has also been verified.
Karijolich
John
J
Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY, USA.
Yu
Yi-Tao
YT
D000818:Animals; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D011560:Pseudouridine / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D017381:Spliceosomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D012342:RNA, Small Nuclear; D011560:Pseudouridine