Genetic analysis of strains of Japanese Encephalitis Virus isolated from swine in central China.
In this study, four strains of Japanese Encephalitis Virus (JEV) were isolated from the cerebrospinal fluid of aborted fetuses or stillborn piglets collected randomly from a number of piggeries in central China. The E genes were cloned by RT-PCR and sequenced. Phylogenetic analysis was performed with 48 JEV isolates previously reported in China and other countries, and showed that all four isolates can be classified into the subcluster of genotype III. The results strongly suggest that the genotype III of JEV is the major variant currently circulating in the swinery of central China.
Fan
Jian-Ming
JM
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002 Zhengzhou, People's Republic of China.
Luo
Jun
J
Chen
Lu
L
Teng
Man
M
Bu
Dan
D
Wang
Fang-Yu
FY
Wang
Li
L
Wang
Chuan-Qing
CQ
Zhang
Gai-Ping
GP
D000818:Animals; D002555:Cerebrospinal Fluid / Q000821:virology; D002681:China; D016000:Cluster Analysis; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000662:veterinary* / Q000821:virology; D008562:Membrane Glycoproteins / Q000235:genetics; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics
D008562:Membrane Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C080628:glycoprotein E, Japanese encephalitis virus