Comparative analysis of Bombyx mori nucleopolyhedrovirus responsive genes in fat body and haemocyte of B. mori resistant and susceptible strains.
The infection profiles of the Bombyx mori nucleopolyhedrovirus (BmNPV) in B. mori larvae revealed that the virus invaded the fat body and haemocyte of both KN and 306 strains, which are highly resistant and susceptible, respectively, to BmNPV infection. However, viral proliferation was notably slowed in the resistant B. mori strain. Using suppression subtractive hybridization, two fat body cDNA libraries were constructed to compare BmNPV responsive gene expression levels between the two silkworm lines. In total, 96 differentially expressed genes were obtained. Real-time quantitative PCR (qPCR) analysis confirmed that eight genes were significantly up-regulated in the fat body and haemocyte of the KN strain following BmNPV injection. Our results suggest that these genes may have potential roles in B. mori antiviral infection mechanisms.
Bao
Y-Y
YY
Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Lv
Z-Y
ZY
Liu
Z-B
ZB
Xue
J
J
Xu
Y-P
YP
Zhang
C-X
CX
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000821:virology*; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005216:Fat Body / Q000378:metabolism / Q000821:virology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017344:Genes, Insect / Q000235:genetics*; D006434:Hemocytes / Q000378:metabolism / Q000821:virology*; D007113:Immunity, Innate / Q000235:genetics*; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D013997:Time Factors; D014779:Virus Replication / Q000235:genetics
D018076:DNA, Complementary