title: Liquid chromatography/tandem mass spectrometry study of anti-inflammatory activity of plantain (Plantago L.) species.
abstract: To evaluate anti-inflammatory activity of selected Plantago species (P. lanceolata L. and P. major L.) an optimized in vitro test for determination of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) inhibition potency was undertaken. By using intact cell system (platelets) as a source of COX-1 and 12-LOX enzymes and highly sensitive and specific LC-MS/MS technique for detection of main arachidonic acid metabolites formed by COX-1 and 12-LOX, this test provides efficient method for evaluation of anti-inflammatory potential of plant extracts and isolated compounds. Our results validated the well-known COX-1 inhibitory activity of P. lanceolata and P. major methanol extracts (concentration required for 50% inhibition (IC(50)) was 2.00 and 0.65 mg/ml, respectively). Furthermore, 12-LOX inhibitory activity of examined extracts was reported for the first time (IC(50)=0.75 and 1.73 mg/ml for P. lanceolata and P. major, respectively). Although renowned inhibitors, such as acetylsalicylic acid and quercetin showed higher activity, this study verifies P. lanceolata and P. major as considerable anti-inflammatory agents.
authors.affiliation: Department of Chemistry, Biochemistry and Environmental Protection, University of Novi Sad, Faculty of Sciences, Trg Dositeja Obradovića 3, Novi Sad, Serbia. ivanak@eunet.rs
authors.forename: Ivana N
authors.initials: IN
authors.lastname: Beara
authors.forename: Dejan Z
authors.initials: DZ
authors.lastname: Orcić
authors.forename: Marija M
authors.initials: MM
authors.lastname: Lesjak
authors.forename: Neda M
authors.initials: NM
authors.lastname: Mimica-Dukić
authors.forename: Biljana A
authors.initials: BA
authors.lastname: Peković
authors.forename: Mira R
authors.initials: MR
authors.lastname: Popović
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002853:Chromatography, Liquid / Q000379:methods*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D006801:Humans; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D010943:Plantago / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000893:Anti-Inflammatory Agents; D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts