Polar localization and degradation of Arabidopsis boron transporters through distinct trafficking pathways.
Boron (B) is essential for plant growth but is toxic when present in excess. In the roots of Arabidopsis thaliana under B limitation, a boric acid channel, NIP5;1, and a boric acid/borate exporter, BOR1, are required for efficient B uptake and subsequent translocation into the xylem, respectively. However, under high-B conditions, BOR1 activity is repressed through endocytic degradation, presumably to avoid B toxicity. In this study, we investigated the localization of GFP-tagged NIP5;1 and BOR1 expressed under the control of their native promoters. Under B limitation, GFP-NIP5;1 and BOR1-GFP localized preferentially in outer (distal) and inner (proximal) plasma membrane domains, respectively, of various root cells. The polar localization of the boric acid channel and boric acid/borate exporter indicates the radial transport route of B toward the stele. Furthermore, mutational analysis revealed a requirement of tyrosine residues, in a probable cytoplasmic loop region of BOR1, for polar localization in various cells of the meristem and elongation zone. The same tyrosine residues were also required for vacuolar targeting upon high B supply. The present study of BOR1 and NIP5;1 demonstrates the importance of selective endocytic trafficking in polar localization and degradation of plant nutrient transporters for radial transport and homeostasis of plant mineral nutrients.
Takano
Junpei
J
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. jtakano@abs.agr.hokudai.ac.jp
Tanaka
Mayuki
M
Toyoda
Atsushi
A
Miwa
Kyoko
K
Kasai
Koji
K
Fuji
Kentaro
K
Onouchi
Hitoshi
H
Naito
Satoshi
S
Fujiwara
Toru
T
D017920:Antiporters / Q000378:metabolism*; D020346:Aquaporins / Q000378:metabolism*; D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001895:Boron / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D004058:Diffusion; D049452:Green Fluorescent Proteins / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism
D017920:Antiporters; D020346:Aquaporins; D029681:Arabidopsis Proteins; C469216:BOR1 protein, Arabidopsis; C512214:NIP5;1 protein, Arabidopsis; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D014443:Tyrosine; D001895:Boron