The cell-free protein synthesis system from wheat germ.
The wheat-germ cell-free protein synthesis system had been one of the most efficient eukaryotic cell-free systems since it was first developed in 1964. However, radio-labeled amino acids had long been essential for detection of the products. Since the discovery of a method for prevention of the contamination by a protein synthesis inhibitor originated from endosperm, the wheat cell-free system has found a wide variety of applications in postgenomic high-throughput screening, structural biology, medicine, and so on. In this chapter, we describe a method for preparation of the cell-free extract and a standard protein synthesis method, as the methods for the applications are found in later chapters.
Takai
Kazuyuki
K
Cell-Free Science and Technology Research Center and Venture Business Laboratory, Ehime University, Ehime, Japan.
Endo
Yaeta
Y
D000818:Animals; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D006801:Humans; D007930:Leucine / Q000378:metabolism; D014176:Protein Biosynthesis; D015202:Protein Engineering / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000196:embryology / Q000235:genetics / Q000378:metabolism*
D002250:Carbon Radioisotopes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007930:Leucine