Isolation, structural characterization and immunological activity of an exopolysaccharide produced by Bacillus licheniformis 8-37-0-1.
A strain of Bacillus licheniformis 8-37-0-1 with high exopolysaccharide (EPS) production ability was isolated and identified based on morphological and physiological characteristics and phylogenetic analysis of 16S rDNA sequences. A new type of EPS was isolated from the strain fermentation broth by enzymolysis, isopropanol precipitation, anion-exchange, and gel-filtration chromatography. The new EPS was determined as homogeneous, with a molecular weight of 2.826 x 10(4), as determined by High-Performance Size-Exclusion Chromatography Multi-Angle Laser Light Scattering analysis. Its structural characteristics were investigated and elucidated by methylation analysis, partial acid hydrolysis, gas-liquid chromatography mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance spectroscopy. Based on obtained data, the EPS was found to be a levan containing a (2-->6)-linked backbone with a single beta-d-fructose at the C-1 position every seven residue, on average, along the main chain. Preliminary in vitro tests revealed that EPS could significantly stimulate the proliferation of spleen lymphocyte.
Liu
Chunhui
C
National Glycoengineering Research Center, Shandong University, Jinan 250012, PR China.
Lu
Juan
J
Lu
Lili
L
Liu
Yuhong
Y
Wang
Fengshan
F
Xiao
Min
M
D001407:Bacillus / Q000378:metabolism*; D002849:Chromatography, Gas / Q000379:methods; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D004798:Enzymes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008954:Models, Biological; D010802:Phylogeny; D011134:Polysaccharides / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000378:metabolism; D012542:Scattering, Radiation; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
D004798:Enzymes; D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S