[Correlation analysis between HPLC fingerprint of Radix Astragali injection and antioxidant activity].
OBJECTIVE
To perform the correlation analysis between HPLC fingerprint of Radix Astragali injection and antioxidant activity.


METHODS
HPLC fingerprints of Radix Astragali injections were developed, and the corresponding DPPH radical-scavenging activities of these injections were also analyzed. The correlation analysis between the HPLC fingerprints of injections and radical-scavenging activities were carried out with PLS method.


RESULTS
Among 14 common chromatographic peaks in HPLC fingerprints of Radix Astragali injections. Peaks 1,3,5,7,8,9,10,11, 12,13 and 14 are positively relevant to IC50 of DPPH radical-scavenging activity. Peaks 1,9,10,12,13 and 14 are important for radical-scavenging activities. Peaks 2,4 and 6 are negatively related to IC50.


CONCLUSIONS
PLS could be a feasible method to investigate the relationship between HPLC fingerprints of Radix Astragali injections and antioxidant activities.
Zhang
Lei
L
School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China. lei05282007@yahoo.com.cn
Nie
Lei
L
Wang
Wei-hong
WH
D027883:Astragalus Plant / Q000737:chemistry*; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology; D016018:Least-Squares Analysis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry
D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D010936:Plant Extracts