title: Modified nano-TiO2 coupled with fluorescence spectroscopy for the separation/analysis of L-tryptophan.
abstract: A novel adsorbent of carboxymethyl-beta-cyclodextrin modified nanometer TiO(2) (CM-beta-CD/TiO(2)) was prepared and used as a solid-phase extraction (SPE) material coupled to fluorescence spectroscopy determination of l-tryptophan (l-Trp) in biological samples. The experimental conditions for modified nanometer TiO(2) separation/preconcentration of l-Trp were optimized. The adsorption capacity of CM-beta-CD/TiO(2) for l-Trp was 75.2 microg/g. The linear range, detection limit (DL), and the relative standard deviation (RSD) were 0.10-1.20 microg/mL, 18.8 ng/mL, and 0.67% (n=3, 1.0 microg/mL), respectively, with a preconcentration factor of 10. The developed method was applied to determination of l-Trp in real samples and the recoveries were found to be in the range of 99.2-100.3%. For validation, a comparison material of NIC-140686 sample was analyzed and the determined value was in good agreement with the certified value.
authors.affiliation: Department of Chemistry, Yangzhou University, Yangzhou 225002, People's Republic of China. xszhu@yzu.edu.cn
authors.forename: Xiashi
authors.initials: X
authors.lastname: Zhu
authors.forename: Ying
authors.initials: Y
authors.lastname: Gu
mesh_terms: D000327:Adsorption; D057230:Limit of Detection; D052616:Solid Phase Extraction / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014025:Titanium / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry; D014364:Tryptophan / Q000032:analysis*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; C441457:carboxymethyl-beta-cyclodextrin; C009495:titanium dioxide; D014364:Tryptophan; D014025:Titanium