title: Proinflammatory cytokines (IL-1beta and TNF-alpha) and chemokines (IL-8 and MIP-1alpha) as markers of peri-implant tissue condition.
abstract: Analysis of peri-implant crevicular fluid (PICF) offers a non-invasive means of studying the host response in peri-implant disease and may provide an early indication of patients at risk for active disease. This study examined the PICF levels of interleukin-1beta (IL-1beta), tumour necrosis factor alpha (TNF-alpha), interleukin-8 (IL-8) and macrophage inflammatory protein-1alpha (MIP-1alpha) in patients with non-manifesting inflammation, early and late stages of mucositis. The study group comprised 90 adult healthy volunteers with endosseal titanium implants inserted. Samples were taken from peri-implant sulcus using a filter paper technique. Implant tissues were categorized clinically as healthy, early mucositis or advanced mucositis. Clinical manifestations were determined by: gingival index and bleeding on probing, plaque index and radiographic analyses. Cytokine concentrations were assesed using commercially available enzyme-linked immunosorbent assay kits. Patients from the control group (healthy patients) have significantly lower concentrations of IL-1beta, TNF-alpha, IL-8 and MIP-1alpha in PICF compared with both groups with mucositis. Positive correlation was noted in the control group between IL-1beta and TNF-alpha and between MIP-1alpha and IL-8 in the group with early mucositis. The results suggest that cytokines could be prognostic markers of implant failure.
authors.affiliation: Clinic for Maxillofacial, Oral Surgery and Implantology, Military Medical Academy, Belgrade, Serbia.
authors.forename: A B
authors.initials: AB
authors.lastname: Petković
authors.forename: S M
authors.initials: SM
authors.lastname: Matić
authors.forename: N V
authors.initials: NV
authors.lastname: Stamatović
authors.forename: D V
authors.initials: DV
authors.lastname: Vojvodić
authors.forename: T M
authors.initials: TM
authors.lastname: Todorović
authors.forename: Z R
authors.initials: ZR
authors.lastname: Lazić
authors.forename: R J
authors.initials: RJ
authors.lastname: Kozomara
mesh_terms: D054405:Chemokine CCL3 / Q000032:analysis; D016207:Cytokines / Q000032:analysis*; D003758:Dental Implantation, Endosseous / Q000009:adverse effects*; D015921:Dental Implants / Q000009:adverse effects*; D019232:Dental Restoration Failure; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis*; D053583:Interleukin-1beta / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D008297:Male; D008875:Middle Aged; D052016:Mucositis / Q000175:diagnosis / Q000209:etiology / Q000473:pathology; D010512:Periodontal Index; D010518:Periodontitis / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology; D013280:Stomatitis / Q000175:diagnosis / Q000209:etiology / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D054405:Chemokine CCL3; D016207:Cytokines; D015921:Dental Implants; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha