[Change of HIF-1alpha protein expression in the placenta bed and concentration of vWF in maternal peripheral blood of pre-eclampsia].
OBJECTIVE
To investigate the expression of HIF-1alpha protein in the placenta bed and the concentration of von Willrand factor (vWF) in maternal peripheral blood from pre-eclampsia and normal pregnancy, and to determine the effect of HIF-1alpha and vWF on the pathogenesis of pre-eclampsia.


METHODS
Forty pre-eclampsia patients (20 mild and 20 severe) were recruited as 2 study groups, and another 20 normal pregnant women were served as a normal group.Western blot was used to detect the expression of HIF-1alpha protein in the placenta bed. ELISA was adopted to detect the concentration of vWF in the maternal peripheral blood.Correlation between HIF-1alpha protein expression and vWF level was analyzed by Spearman method.


RESULTS
The expression of HIF-1alpha protein in the placenta bed was the highest in the severe pre-eclampsia patients among 3 groups, followed by the mild pre-eclampsia patients and the normal controls. There was significant difference among 3 groups (P<0.001). The concentration of vWF in the maternal peripheral blood was the highest in the severe pre-eclampsia patients, followed by the mild pre-eclampsia patients and the normal controls. There was significant difference among the 3 groups (P<0.05). Positive correlation was found between the expression of HIF-1alpha protein in the placenta bed and the concentration of vWF in the maternal peripheral blood in patients with pre-eclampsia (r1=0.65, P<0.001), and between the concentration of vWF in the maternal peripheral blood and the pathogenetic condition degree of pre-eclampsia (r(2)=0.61,P<0.001).


CONCLUSIONS
HIF-1alpha in coordination with vWF may play an important role in the pathogenesis of pre-eclampsia.
Peng
Mei
M
Department of Gynecology and Obstetrics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Ding
Yiling
Y
Yu
Ling
L
Zhou
Changju
C
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000097:blood / Q000209:etiology / Q000378:metabolism*; D011247:Pregnancy; D014841:von Willebrand Factor / Q000378:metabolism*
C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D014841:von Willebrand Factor