Early homing behavior of Stro-1- mesenchyme-like cells derived from human embryonic stem cells in an immunocompetent xenogeneic animal model.
Mesenchymal stem cells (MSCs) have been induced to differentiate successfully from human embryonic stem cells (hES-MSCs), which could serve as an in vitro source of MSCs. However, the homing behaviors of such cells and their potential utility for liver regeneration in vivo have not been reported. We investigated factors that influenced early homing and the hepatic-directed differentiation potency of hES-MSCs in a mouse model of acute liver injury. The hES-MSCs could be detected 36 h after cell infusion and this was unaffected by the number of cell passages in culture. Pretreatment of hES-MSCs with TNF-alpha resulted in higher rates of homing of these cells to the injured liver. Interestingly most of the cells homing at an early stage expressed alpha-fetoprotein (AFP), indicating hepatic differentiation. Thus, hES-MSCs can home to the acutely injured liver at high efficiency and undergo hepatic differentiation, suggesting that these cells could be useful for treating acute human liver injury.
Su
Zhongyuan
Z
College of Life Sciences, Zhejiang University, No. 338, Yu-Hang-Tang Road, Hangzhou, Zhejiang 310058, PR China.
Wu
Rongrong
R
Tan
Zhou
Z
Li
Ying
Y
Chen
Liangbiao
L
Luo
Jingfeng
J
Zhang
Ming
M
D000577:Amides / Q000494:pharmacology; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation; D053595:Embryonic Stem Cells / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007121:Immunocompetence; D008099:Liver / Q000166:cytology / Q000293:injuries* / Q000502:physiology; D008115:Liver Regeneration; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008813:Mice, Inbred ICR; D011725:Pyridines / Q000494:pharmacology; D019707:Receptors, Chemokine / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D000509:alpha-Fetoproteins / Q000096:biosynthesis
D000577:Amides; D000954:Antigens, Surface; D011725:Pyridines; D019707:Receptors, Chemokine; C525591:STRO-1 antigen, human; D014409:Tumor Necrosis Factor-alpha; D000509:alpha-Fetoproteins; C108830:Y 27632