title: The effects of radix curcumae extract on expressions of VEGF, COX-2 and PCNA in gastric mucosa of rats fed with MNNG.
abstract: OBJECTIVE
  To study the effects of an extract solution from radix curcumae on the expressions of VEGF, COX-2 and PCNA in gastric mucosa of rats during carcinogenesis induced MNNG.
  
  
  METHODS
  Eighty male Wistar rats were randomly divided into 5 groups: group A, water and normal saline; group B, MNNG and normal saline; group C, MNNG and celecoxib; group D, MNNG and low-dose (1 g/ml) radix curcumae steam distillation extract solution; group E, MNNG and high-dose (2g/ml) radix curcumae wet distillation extract solution. In the end of the 40(th) week, all rats alive were sacrificed and the expressions of VEGF, COX-2 and PCNA in gastric mucosa were determined by immunohistochemistry.
  
  
  RESULTS
  The expression levels of VEGF and COX-2 and the optical density levels of PCNA in group C, D and E were remarkably lower than that of group B (P<0.05), while the optical density levels of PCNA in group E were higher than that of group C and D (P<0.05).
  
  
  CONCLUSIONS
  The distilled extract of curcumae can down-regulate the expressions of VEGF, COX-2 and PCNA in the gastric mucosa of rats during carcinogenesis induced MNNG and can reduce the incidence of gastric caner, suggesting it maybe as a potential chemopreventive agent for gastric cancer.
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
authors.forename: Linfeng
authors.initials: L
authors.lastname: Yu
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.forename: Hanqing
authors.initials: H
authors.lastname: Chen
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage; D002273:Carcinogens; D030024:Curcuma / Q000737:chemistry*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D008769:Methylnitronitrosoguanidine; D010936:Plant Extracts / Q000008:administration & dosage*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D016896:Treatment Outcome; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002273:Carcinogens; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A; D008769:Methylnitronitrosoguanidine; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2