[Development and use of a domestic test system for diagnosis of tuberculous infection on the basis of quantitative analysis of interferon-gamma induction in whole blood samples in vitro, by using specific recombinant antigens].
A test system was developed to detect tuberculous infection by qualitative analysis of interferon-gamma (IFN-gamma) in the plasma samples after 20-24-hour incubation of whole blood samples in the presence of Mycobacterium tuberculosis (MBT) antigens: tuberculin PPD and a mixture of the MBT-specific recombinant antigens ESAT-6 and CFP-10. The analysis used 3 test tubes each containing 1 ml of heparinized venous blood, one of which served as a control; the other two test tubes were employed to measure antigen-induced IFN-gamma production. Whether this test system might be used to determine primary tuberculous infection was studied in 277 children and adolescents. The threshold diagnostic IFN-gamma induction level determined in the test tube containing a mixture of the antigens ESAT-6 and CFP-10 was ascertained. Postvaccine allergy was detectable if there was IFN-gamma induction in the test tube containing tuberculin and if there was no diagnostic IFN-gamma level in that containing the antigens ESAT-6 and CFP-10. The diagnostic sensitivity of detection of primary tuberculous infection was 97.6% with 94.4% specificity, which enabled this condition to be differentiated from postvaccine allergy. The level of antigen-induced IFN-gamma may be lower in relatively disseminated forms of pulmonary tuberculosis.
Vladimirskiĭ
M A
MA
Mordovskaia
L I
LI
Aksenova
V A
VA
Shipina
L K
LK
Aksenova
E I
EI
Sergienko
O V
OV
Liashuk
A M
AM
Neretina
T V
TV
Kariagina
A S
AS
Lunin
V G
VG
Efremov
E E
EE
Ignashenkova
G V
GV
Vlasik
T N
TN
Ianushevskaia
E V
EV
Kashirina
N M
NM
D000293:Adolescent; D000942:Antigens, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000097:blood*; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000097:blood; D012680:Sensitivity and Specificity; D014373:Tuberculin / Q000097:blood; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000175:diagnosis*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; D014373:Tuberculin; D007371:Interferon-gamma