title: Differentiation, distribution and gammadelta T cell-driven regulation of IL-22-producing T cells in tuberculosis.
abstract: Differentiation, distribution and immune regulation of human IL-22-producing T cells in infections remain unknown. Here, we demonstrated in a nonhuman primate model that M. tuberculosis infection resulted in apparent increases in numbers of T cells capable of producing IL-22 de novo without in vitro Ag stimulation, and drove distribution of these cells more dramatically in lungs than in blood and lymphoid tissues. Consistently, IL-22-producing T cells were visualized in situ in lung tuberculosis (TB) granulomas by confocal microscopy and immunohistochemistry, indicating that mature IL-22-producing T cells were present in TB granuloma. Surprisingly, phosphoantigen HMBPP activation of Vgamma2Vdelta2 T cells down-regulated the capability of T cells to produce IL-22 de novo in lymphocytes from blood, lung/BAL fluid, spleen and lymph node. Up-regulation of IFNgamma-producing Vgamma2Vdelta2 T effector cells after HMBPP stimulation coincided with the down-regulated capacity of these T cells to produce IL-22 de novo. Importantly, anti-IFNgamma neutralizing Ab treatment reversed the HMBPP-mediated down-regulation effect on IL-22-producing T cells, suggesting that Vgamma2Vdelta2 T-cell-driven IFNgamma-networking function was the mechanism underlying the HMBPP-mediated down-regulation of the capability of T cells to produce IL-22. These novel findings raise the possibility to ultimately investigate the function of IL-22 producing T cells and to target Vgamma2Vdelta2 T cells for balancing potentially hyper-activating IL-22-producing T cells in severe TB.
authors.affiliation: Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, United States of America.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Yao
authors.forename: Dan
authors.initials: D
authors.lastname: Huang
authors.forename: Crystal Y
authors.initials: CY
authors.lastname: Chen
authors.forename: Lisa
authors.initials: L
authors.lastname: Halliday
authors.forename: Gucheng
authors.initials: G
authors.lastname: Zeng
authors.forename: Richard C
authors.initials: RC
authors.lastname: Wang
authors.forename: Zheng W
authors.initials: ZW
authors.lastname: Chen
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002469:Cell Separation; D005434:Flow Cytometry; D007150:Immunohistochemistry; D020381:Interleukin-17 / Q000096:biosynthesis / Q000276:immunology; D007378:Interleukins / Q000096:biosynthesis / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008251:Macaca; D018613:Microscopy, Confocal; D009169:Mycobacterium tuberculosis / Q000276:immunology; D010755:Organophosphates / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D014376:Tuberculosis / Q000276:immunology* / Q000378:metabolism; D000097043:Interleukin-22
chemical_list: D000942:Antigens, Bacterial; D020381:Interleukin-17; D007378:Interleukins; D010755:Organophosphates; D016692:Receptors, Antigen, T-Cell, gamma-delta; D000097043:Interleukin-22; C467061:4-hydroxy-3-methyl-2-butenyl diphosphate