Spectroscopic investigation on the toxic interaction of melamine with herring sperm DNA.
The toxic interaction of melamine with herring sperm DNA (hs-DNA) was investigated by using fluorescence and UV-vis absorption spectra techniques. The experimental results showed that the toxic interaction between melamine and hs-DNA occurred. Fluorescence quenching experiments indicated the existence of electrostatic binding between melamine and hs-DNA. The binding constants K(A) and the binding site numbers were calculated by means of the Stern-Volmer equation and were 9.8 × 10(4) L mol(-1) and 1.3, respectively. Both the results of fluorescence spectra and UV-vis absorption spectra verified that there are electrostatic binding between melamine and hs-DNA. The possibility in the presence of a classical intercalation binding mode could be ruled out by using DNA unwinding experiments.
Sun
Yajing
Y
School of Environmental Science and Engineering, Shandong University, Jinan 250100, People's Republic of China.
Liu
Rutao
R
Chi
Zhenxing
Z
Qin
Pengfei
P
Fang
Xiaoyan
X
Mou
Yue
Y
D000042:Absorption; D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005399:Fishes; D005453:Fluorescence; D007364:Intercalating Agents / Q000378:metabolism; D008297:Male; D012117:Resins, Synthetic / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013057:Spectrum Analysis / Q000379:methods; D013094:Spermatozoa / Q000378:metabolism*; D055672:Static Electricity / Q000009:adverse effects; D014227:Triazines / Q000737:chemistry*
D007364:Intercalating Agents; D012117:Resins, Synthetic; D014227:Triazines; D004247:DNA; C011907:melamine