Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon.
The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.
Tek
Ahmet L
AL
Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan. altek2@gmail.com
Kashihara
Kazunari
K
Murata
Minoru
M
Nagaki
Kiyotaka
K
D000595:Amino Acid Sequence; D000918:Antibody Specificity / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D002503:Centromere / Q000235:genetics*; D006657:Histones / Q000737:chemistry / Q000235:genetics; D047468:Immunoprecipitation; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D018626:Retroelements / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics*
D006657:Histones; D018626:Retroelements