Protein-protein and protein-lipid interactions in domain-assembly: lessons from giant unilamellar vesicles.
Giant Unilamellar Vesicles (GUVs) provide a key model membrane system to study lipid-lipid and lipid-protein interactions, which are relevant to vital cellular processes, by (single-molecule) optical microscopy. Here, we review the work on reconstitution techniques for membrane proteins and other preparation methods for developing GUVs towards most suitable close-to-native membrane systems. Next, we present a few applications of protein-containing GUVs to study domain assembly and protein partitioning into raft-like domains.
Kahya
Nicoletta
N
Department of Cell Biology, University Medical Center Groningen, Groningen, The Netherlands. N.N.Kahya@med.umcg.nl
D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D008954:Models, Biological; D008956:Models, Chemical; D053835:Unilamellar Liposomes / Q000737:chemistry*
D008565:Membrane Proteins; D053835:Unilamellar Liposomes