title: A practical approach to RT-qPCR-Publishing data that conform to the MIQE guidelines.
abstract: Given the highly dynamic nature of mRNA transcription and the potential variables introduced in sample handling and in the downstream processing steps (Garson et al. (2009)), a standardized approach to each step of the RT-qPCR workflow is critical for reliable and reproducible results. The MIQE provides this approach with a checklist that contains 85 parameters to assure quality results that will meet the acceptance criteria of any journal (Bustin et al. (2009)). In this paper we demonstrate how to apply the MIQE guidelines (www.rdml.org/miqe) to establish a solid experimental approach.
authors.affiliation: Bio-Rad Laboratories, Inc., Hercules, CA 94547, USA.
authors.forename: Sean
authors.initials: S
authors.lastname: Taylor
authors.forename: Michael
authors.initials: M
authors.lastname: Wakem
authors.forename: Greg
authors.initials: G
authors.lastname: Dijkman
authors.forename: Marwan
authors.initials: M
authors.lastname: Alsarraj
authors.forename: Marie
authors.initials: M
authors.lastname: Nguyen
mesh_terms: D017408:Guidelines as Topic; D011643:Publishing / Q000592:standards*; D011786:Quality Control; D012313:RNA / Q000592:standards; D012107:Research Design / Q000592:standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D054928:Validation Studies as Topic
chemical_list: C034477:RNA primers; D012313:RNA