title: Isolation of amniotic mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) have the ability to differentiate into osteocytes, chondrocytes, and adipocytes and possess immunomodulatory properties. Amniotic membrane from human term placenta is a potential source of multipotent MSCs that could be useful for regenerative medicine. This unit describes a detailed and simple protocol for the isolation of amniotic mesenchymal cells. We also introduce a simple density separation technique for the purification of this cell type from possible contamination with amniotic epithelial cells.
authors.affiliation: University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Fabio
authors.initials: F
authors.lastname: Marongiu
authors.forename: Roberto
authors.initials: R
authors.lastname: Gramignoli
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Veysel
authors.initials: V
authors.lastname: Tahan
authors.forename: Toshio
authors.initials: T
authors.lastname: Miki
authors.forename: Kenneth
authors.initials: K
authors.lastname: Dorko
authors.forename: Ewa
authors.initials: E
authors.lastname: Ellis
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Strom
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000650:Amnion / Q000166:cytology*; D002452:Cell Count; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D039902:Multipotent Stem Cells; D010011:Osteocytes / Q000166:cytology; D010920:Placenta; D011247:Pregnancy; D044968:Regenerative Medicine