title: Epidemiology of Chlamydophila caviae-like Chlamydia isolated from urethra and uterine cervix.
abstract: In 2000, chlamydial strains OK133 and OK135 were isolated from 2 female patients with cervicitis. These strains were unresponsive to commercially available PCR and LCR test kits for the diagnosis of Chlamydia trachomatis infection, and their phenotypic characteristics were very similar. The OK135 nucleotide sequence in MOMP-VD2 gene closely resembled that of Chlamydophila caviae GPIC. A similar strain was isolated in 2003 from a male patient OKM2 with urethritis, from which the strain SC10-6 was cloned by the plaque purification method. The nucleotide sequence of the entire MOMP gene of SC10-6 was exactly the same as that of OK135. Thus, the strains OK135 and SC10-6, together with OK133, have been called C. caviae-like Chlamydia. We designed primers for nested PCR assay, the product of which showed a single-band 311-bp fragment, to detect C. caviae-like Chlamydia. Of swab specimens obtained from 202 patients from 2003 to 2006 (119 male and 83 female patients), 18 specimens (8.9%) from 14 male and 4 female patients were positive, suggesting that C. caviae-like Chlamydia infection is rather common. Thus far, it has not been determined whether C. caviae-like Chlamydia is pathogenic for humans.
authors.affiliation: Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Murao
authors.forename: Koichiro
authors.initials: K
authors.lastname: Wada
authors.forename: Akira
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Michihisa
authors.initials: M
authors.lastname: Fujiwara
authors.forename: Hideto
authors.initials: H
authors.lastname: Fukushi
authors.forename: Toshio
authors.initials: T
authors.lastname: Kishimoto
authors.forename: Koichi
authors.initials: K
authors.lastname: Monden
authors.forename: Reiko
authors.initials: R
authors.lastname: Kariyama
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kumon
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000453:epidemiology* / Q000382:microbiology; D023501:Chlamydophila / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D014521:Urethra / Q000382:microbiology; D014526:Urethritis / Q000453:epidemiology* / Q000382:microbiology; D002575:Uterine Cervicitis / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins