Analysis of NSAID-activated gene 1 expression in prostate cancer.
Nonsteroidal anti-inflammatory drugs (NSAIDs) induce expression of NSAID-activated gene 1 (NAG-1). NAG-1 expression in prostate cancer has previously been detected by microarray and immunohistochemical analyses. We examined the mRNA expression of NAG-1 by quantitative real-time polymerase chain reaction using 51 human prostate cancer tissue specimens. The level of NAG-1 expression was higher in prostate cancer than in normal prostate tissues (p = 0.025). The level of NAG-1 expression was also significantly higher in well-differentiated prostate cancer than in moderately (p = 0.04) and poorly differentiated adenocarcinomas (p = 0.03). These data suggest that NAG-1 may be a new biomarker for evaluating the grade of malignancy in prostate cancer.
Kawahara
Takashi
T
Department of Urology, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
Ishiguro
Hitoshi
H
Hoshino
Koji
K
Teranishi
Jun-Ichi
J
Miyoshi
Yasuhide
Y
Kubota
Yoshinobu
Y
Uemura
Hiroji
H
D000199:Actins / Q000378:metabolism; D000230:Adenocarcinoma / Q000473:pathology; D000368:Aged; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002454:Cell Differentiation; D015972:Gene Expression Regulation, Neoplastic; D055436:Growth Differentiation Factor 15 / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011467:Prostate / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000199:Actins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D014408:Biomarkers, Tumor; C526140:GDF15 protein, human; D055436:Growth Differentiation Factor 15