Effect of aluminium uptake on physiology, phenols and amino acids in Matricaria chamomilla plants.
Chamomile is a widely used medicinal plant and, as observed in our previous studies, also accumulates some metals in its above-ground biomass. We therefore tested selected metabolic responses after treatments with 60 and 120 microM Al for 7 days. Shoot Al content was not elevated in comparison with control (12.3-14.1 microg g(-1) DW) while total root Al increased strongly, reaching 2680 and 4400 microg g(-1) DW in 60 and 120 microM treatments, respectively. "Intra-root" Al represented 83.6 (60 microM treatment) and 75.8% (120 microM treatment) of total root Al. Soluble proteins were not significantly affected. Free amino acids were almost unaffected in shoots while in roots the highest content was found in 60 microM Al. Ascorbate- and guaiacol-peroxidase activities were the highest in 60 microM Al-exposed roots. On the other hand, phenylalanine ammonia-lyase activity, total soluble phenols, flavonoids, a sum of 13 phenolic acids and partially two flavonols (quercetin and kaempferol) increased in the shoots. Present study has shown lower Al toxicity and unaltered shoot Al content seems to be the most positive outcome in comparison with previously tested metals (Cd, Ni and Cu). Our results indicate that phenols in shoots and free amino acids in roots are influenced by Al excess in chamomile plants. Possible mechanisms in the context of available literature are suggested and discussed.
Kovácik
Jozef
J
Department of Botany, Institute of Biology and Ecology, Faculty of Science, P. J. Safárik University, Mánesova 23, 041 67 Kosice, Slovak Republic. jozkovacik@yahoo.com
Klejdus
Borivoj
B
Hedbavny
Josef
J
D000535:Aluminum / Q000378:metabolism* / Q000633:toxicity*; D000596:Amino Acids / Q000378:metabolism*; D018533:Biomass; D005502:Food; D062385:Hydroxybenzoates / Q000737:chemistry; D008031:Lignin / Q000737:chemistry; D008315:Malondialdehyde / Q000737:chemistry; D031168:Matricaria / Q000235:genetics / Q000378:metabolism* / Q000633:toxicity; D008903:Minerals / Q000737:chemistry; D010544:Peroxidases / Q000032:analysis / Q000737:chemistry; D010636:Phenols / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000737:chemistry; D036226:Seedlings
D000596:Amino Acids; D062385:Hydroxybenzoates; D008903:Minerals; D010636:Phenols; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D008031:Lignin; D000535:Aluminum; D010544:Peroxidases; C017616:phenolic acid