Purification and structure determination of gelatinase and collagenase inhibitors from Viola patrinii fermentation extracts.
Investigation of collagenase and gelatinase inhibitory natural components afforded two isoflavonoids. Two isoflavonoids, tectorigenin-7-O-β-D-glucoside (1) and luteolin-7-O-β-D-glucuronopyranoside (2), were isolated from ethyl acetate fraction of Viola patrinii fermentation extracts (VPFE). Of these, compounds 1 and 2 exhibited collagenase inhibitory activity (IC(50)) at a concentration of less than 1.5 μM, and compound 2 showed gelatinases A and B inhibitory activity (IC(50)) at 0.3 μM and 0.8 μM, respectively.
Kim
Kyoung-Sook
KS
College of Natural Resources and Life Science, BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714, South Korea.
Kwak
Yeon-Joo
YJ
Kim
Kyung-Mi
KM
Yu
Hai Yang
HY
Kang
Byoung-Won
BW
Chung
Eunsook
E
Lee
Young-Choon
YC
Kim
Jung-In
JI
Lee
Jai-Heon
JH
D055162:Biocatalysis / Q000187:drug effects; D002845:Chromatography / Q000379:methods; D017364:Collagenases / Q000378:metabolism; D005285:Fermentation; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018093:Gelatinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D020128:Inhibitory Concentration 50; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D047311:Luteolin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D011480:Protease Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D032413:Viola / Q000378:metabolism / Q000382:microbiology*
D005419:Flavonoids; D007529:Isoflavones; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D011480:Protease Inhibitors; C456096:luteolin-7-O-glucuronide; C120040:tectoridin; D017364:Collagenases; D018093:Gelatinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D047311:Luteolin