The integrity of colonic anastomoses following the intraperitoneal administration of oxaliplatin.
BACKGROUND
The purpose of this experimental study was to determine the effect of oxaliplatin on the integrity of colonic anastomoses which were under oxaliplatin administration.


METHODS
Thirty rats were randomized to two groups. After resection of a 1-cm segment of the transverse colon, an end-to-end sutured anastomosis was performed. Rats of the control group were injected with 3 ml of 0.9% sodium chloride solution and in the oxaliplatin group with 2.4 mg/kg of oxaliplatin intraperitoneally immediately after surgery and for seven postoperative days. All rats were sacrificed on the tenth postoperative day, and the anastomoses were examined macroscopically and graded histologically. Rats were measured for anastomotic bursting pressures and tissue hydroxyproline levels.


RESULTS
The body weight changes were significantly greater in the oxaliplatin group (p = 0.005). Anastomotic dehiscence occurred only in the oxaliplatin group. The adhesion formation was significantly increased in the group of oxaliplatin compared to the control group (p = 0.001). The colonic bursting pressure was significantly lower in the oxaliplatin group compared to the control group (p < 0.001). The mean inflammatory cell infiltration was significantly lower in the oxaliplatin group (1.00 vs. 2.33, p < 0.001). The mean neoagiogenesis was significantly lower in the oxaliplatin group (0.80 vs. 2.20, p < 0.001). The mean collagen deposition was significantly lower in the oxaliplatin group and the mean fibroblast activity was significantly lower in the oxaliplatin group (1.27 vs. 2.53, p < 0.001). Hydroxyproline concentration was significantly lower in the oxaliplatin group (p < 0.001).


CONCLUSIONS
Intra- and postoperative intraperitoneal administration of oxaliplatin definitely impairs healing of colonic anastomoses in rats.
Blouhos
Konstantinos
K
Fourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece. kostasblu@hotmail.com
Pramateftakis
Manousos-Georgios
MG
Tsachalis
Theodor
T
Kanellos
Dimitrios
D
Zaraboukas
Thomas
T
Koliakos
Georgios
G
Betsis
Dimitrios
D
D000268:Adhesiveness / Q000187:drug effects; D000714:Anastomosis, Surgical; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003106:Colon / Q000187:drug effects* / Q000473:pathology / Q000601:surgery*; D006909:Hydroxyproline / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008297:Male; D009944:Organoplatinum Compounds / Q000008:administration & dosage* / Q000494:pharmacology*; D000077150:Oxaliplatin; D011184:Postoperative Period; D051381:Rats; D017208:Rats, Wistar
D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D006909:Hydroxyproline