title: Discrimination analysis of human lung cancer cells associated with histological type and malignancy using Raman spectroscopy.
abstract: The Raman spectroscopic technique enables the observation of intracellular molecules without fixation or labeling procedures in situ. Raman spectroscopy is a promising technology for diagnosing cancers-especially lung cancer, one of the most common cancers in humans-and other diseases. The purpose of this study was to find an effective marker for the identification of cancer cells and their malignancy using Raman spectroscopy. We demonstrate a classification of cultured human lung cancer cells using Raman spectroscopy, principal component analysis (PCA), and linear discrimination analysis (LDA). Raman spectra of single, normal lung cells, along with four cancer cells with different pathological types, were successfully obtained with an excitation laser at 532 nm. The strong appearance of bands due to cytochrome c (cyt-c) indicates that spectra are resonant and enhanced via the Q-band near 550 nm with excitation light. The PCA loading plot suggests a large contribution of cyt-c in discriminating normal cells from cancer cells. The PCA results reflect the nature of the original cancer, such as its histological type and malignancy. The five cells were successfully discriminated by the LDA.
authors.affiliation: Aoyama Gakuin University, College of Science and Engineering, Department of Chemistry and Biological Science, Sagamihara, Kanagawa, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Oshima
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Shinzawa
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Takenaka
authors.forename: Chie
authors.initials: C
authors.lastname: Furihata
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Sato
mesh_terms: D014408:Biomarkers, Tumor / Q000737:chemistry; D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000737:chemistry; D016002:Discriminant Analysis; D006801:Humans; D008175:Lung Neoplasms / Q000145:classification / Q000175:diagnosis / Q000473:pathology*; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D045304:Cytochromes c