title: Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins.
abstract: BACKGROUND
  The human immunodeficiency virus type 1 (HIV-1) pandemic has continued unabated for nearly 30 years. To better understand the influence of virus on host cells, we performed the differential proteome research of peripheral blood mononuclear cells (PBMCs) from HIV-positive patients and healthy controls.
  
  
  RESULTS
  26 protein spots with more than 1.5-fold difference were detected in two dimensional electrophoresis (2DE) gels. 12 unique up-regulated and one down-regulated proteins were identified in HIV-positive patients compared with healthy donors. The mRNA expression of 10 genes was analyzed by real time RT-PCR. It shows that the mRNA expression of talin-1, vinculin and coronin-1C were up-regulated in HIV positive patients and consistent with protein expression. Western blotting analysis confirmed the induction of fragments of vinculin, talin-1 and filamin-A in pooled and most part of individual HIV-positive clinical samples. Bioinformatic analysis showed that a wide host protein network was disrupted in HIV-positive patients.
  
  
  CONCLUSIONS
  Together, this work provided useful information to facilitate further investigation of the underlying mechanism of HIV and host cell protein interactions, and discovered novel potential biomarkers such as fragment of vinculin, filamin-A and talin-1 for anti-HIV research.
authors.affiliation: Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Jia
authors.affiliation: Neurosurgery, Fuzhou General Hospital, Fuzhou 350025, PR China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Sun
authors.affiliation: Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Peng
authors.affiliation: Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
authors.forename: Tangkai
authors.initials: T
authors.lastname: Qi
authors.affiliation: Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
authors.affiliation: Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yin
authors.affiliation: Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
authors.forename: Yamin
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Qiu
authors.affiliation: Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
authors.forename: Hongzhou
authors.initials: H
authors.lastname: Lu