title: Crystallization and preliminary X-ray crystallographic study of phosphoglucose isomerase from Plasmodium falciparum.
abstract: Phosphoglucose isomerase (PGI) is a key enzyme in glycolysis and glycogenesis that catalyses the interconversion of glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). For crystallographic studies, PGI from the human malaria parasite Plasmodium falciparum (PfPGI) was overproduced in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data to 1.5 A resolution were collected from an orthorhombic crystal form belonging to space group P2(1)2(1)2(1) with unit-cell parameters a = 103.3, b = 104.1, c = 114.6 A. Structural analysis by molecular replacement is in progress.
authors.affiliation: School of Pharmacy, Showa University, Tokyo 142-8555, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Aoki
authors.forename: Nobutada
authors.initials: N
authors.lastname: Tanaka
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kusakabe
authors.forename: Chiharu
authors.initials: C
authors.lastname: Fukumi
authors.forename: Arayo
authors.initials: A
authors.lastname: Haga
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nakanishi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kitade
authors.forename: Kazuo T
authors.initials: KT
authors.lastname: Nakamura
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D005956:Glucose-6-Phosphate Isomerase / Q000737:chemistry* / Q000302:isolation & purification; D010963:Plasmodium falciparum / Q000201:enzymology*
chemical_list: D005956:Glucose-6-Phosphate Isomerase