Nestin action during insulin-secreting cell differentiation.
Nestin, which was initially identified as a marker of neural stem cells, has been reported in regenerating pancreas as well as in early embryonic stem (ES) cell derivatives. However, little is known about its specific roles in stem cells as a functional regulator. We investigated the source of the action of nestin in ES and adult pancreatic ductal stem (PDS) cells in regard to the neogenesis of insulin-secreting beta-cells. In ES cells, suppression of nestin by gene silencing led to an increased expression of the pluripotency-associated genes, including Oct 4, Nanog, and SSEA-1, before embryoid body (EB) formation, whereas it reduced endodermal and pancreatic transcription factors in EBs. Inhibition of nestin expression in adult PDS cells caused a low expression of pancreatic transcription factors and islet hormones, leading to poor beta-cell development and insulin secretion. These data may indicate not only that nestin is a simple stem cell marker, but also that it constitutes a functional factor at the time of stem cell differentiation. We suggest that nestin plays pivotal roles as an intermediate regulator governing both stemness and differentiation of stem cells in the process of their differentiation into insulin-secreting cells.
Kim
So-Yoon
SY
Department of Anatomy and BK21 Center for Advanced Medical Education, College of Medicine, Inha University, Shinheung-Dong, Jung-Gu, Incheon, 400-103, Korea.
Lee
Song
S
Hong
Seok-Woo
SW
Min
Bon-Hong
BH
Lee
Ki-Up
KU
Bendayan
Moise
M
Park
In-Sun
IS
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D017931:DNA Primers; D005786:Gene Expression Regulation; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000235:genetics; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000235:genetics* / Q000502:physiology; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000502:physiology; D064231:Nestin; D010183:Pancreatic Ducts / Q000166:cytology* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000502:physiology
D017931:DNA Primers; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; C577486:Nes protein, rat; D064231:Nestin; D034741:RNA, Small Interfering; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)