title: Cross-sectional survey on immunoglobulin E reactivity in 23,077 subjects using an allergenic molecule-based microarray detection system.
abstract: BACKGROUND
  The availability of allergenic molecules and high-throughput microtechnologies allow the collection of a large number of IgE results at the same time in a single test. This can be carried out applying the test in the routine diagnostic work-up.
  
  
  OBJECTIVE
  The aim of this study was to make a cross-sectional evaluation of the raw prevalence of IgE reactivity to allergenic molecules in serum samples from a cohort of Italian patients using an innovative tool.
  
  
  METHODS
  The ISAC, a microarray system, has been used for specific IgE detection using 75 different allergenic molecules. Sera were collected from 23,077 unselected consecutive individuals complaining about any allergic disease.
  
  
  RESULTS
  Sixteen thousand four hundred and eight of 23,077 patients had IgE to at least one of 75 allergenic molecules. The top-ranked molecules in this cohort were Cup a 1 (42.7%), Der f 2 (38.7%), and Phl p 1 (37.9%), whereas all the other allergens tested scored in a range between 36.8% and 0.04%, including the first food allergen, Pru p 3, ranked 15th (9.79%). Prevalence varied quite markedly depending on the age range considered, and showing a different behaviour in the lifetime sensitization process. Unsupervised two-way hierarchical clustering analysis generated distinctive patterns of reactivity as the result of IgE recognition of either homologous allergens belonging to different biological sources or non-homologous belonging to the same biological source.
  
  
  CONCLUSIONS
  Allergen-based microarray is a tool for the detection of IgE-related sensitization to panels of allergens and gives a more precise and comprehensive evaluation for an IgE-based epidemiology. This insight brings data for better understanding of the sensitization process.
authors.affiliation: Centre for Clinical and Experimental Allergology, IDI-IRCCS, Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Scala
authors.forename: C
authors.initials: C
authors.lastname: Alessandri
authors.forename: M L
authors.initials: ML
authors.lastname: Bernardi
authors.forename: R
authors.initials: R
authors.lastname: Ferrara
authors.forename: P
authors.initials: P
authors.lastname: Palazzo
authors.forename: D
authors.initials: D
authors.lastname: Pomponi
authors.forename: D
authors.initials: D
authors.lastname: Quaratino
authors.forename: C
authors.initials: C
authors.lastname: Rasi
authors.forename: A
authors.initials: A
authors.lastname: Zaffiro
authors.forename: D
authors.initials: D
authors.lastname: Zennaro
authors.forename: A
authors.initials: A
authors.lastname: Mari
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000485:Allergens / Q000235:genetics / Q000276:immunology; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000453:epidemiology* / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D007558:Italy / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017678:Sex Distribution; D055815:Young Adult
chemical_list: D000485:Allergens; D007073:Immunoglobulin E