title: Issues surrounding standard cytotoxicity testing for assessing activity of non-covalent DNA-binding metallo-drugs.
abstract: Investigating the methods commonly used to evaluate in vitro cytotoxicity of novel compounds, specifically non-covalent DNA binders, identifies that these methods may not be appropriate. The level of anticancer activity depends not only on the incubation time but also on the absolute amount (number of moles) of drug compound applied, rather than the concentration.
authors.affiliation: School of Chemistry, University of Birmingham, Birmingham, UK B91 2ES.
authors.forename: Alexandra J
authors.initials: AJ
authors.lastname: Pope
authors.forename: Christopher
authors.initials: C
authors.lastname: Bruce
authors.forename: Boris
authors.initials: B
authors.lastname: Kysela
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Hannon
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000633:toxicity; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000737:chemistry* / Q000633:toxicity; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007097:Imines / Q000737:chemistry; D007501:Iron / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D007097:Imines; D004247:DNA; D007501:Iron