[Erythromycin ethylsuccinate obtaining possibilities].
In this study we tried to improve the erythromycin ethylsuccinate obtaining, having in view to separate the erythromycin ester by crystallization in water.


METHODS
The erythromycin acylation and the erythromycin ethylsuccinate crystallization were realized, following the next steps: 1. the acylation of the erythromycin with a methylene chloride solution of monoethylsuccinyl chloride, at 25-28 degrees C for 3 hours in the presence of NaHCO3; 2. the transfer of the erythromycin ethylsuccinate from methylene chloride solution in acetone solution by distillation of mixture methylene chloride: acetone 1:1 at 25-28 degrees C; 3. erythromycin ethylsuccinate separation by crystallization in water at pH = 8-8.5 and 5 degrees C for 90 minutes. The quality control for the erythromycin ester was performed according to the Xth edition of Romanian Pharmacopoeia standards using national standard for erythromycin ethylsuccinate and national standard for erythromycin with an activity of 1: 937 U and 2.02% humidity. The Micrococcus luteus ATCC 9341 was used as a test microorganism and a thin layer cromatography was performed for qualitative control.


RESULTS
13.1 g of erythromycin ethylsuccinate were obtained with an output of the process of 82.02%. Using water for the separation of erythromycin ethylsuccinate the output of the process is greater (82.02%) than in case of using petroleum ether (74.14%) or hexane (80.25%). The thin layer cromatography revealed an Rf = 0.56 and the microbiological activity of the erythromycin ethylsuccinate was 98.7% compared with the standard.


CONCLUSIONS
Using water instead of hexane or petroleum ether is gainful for the separation of erythromycin ethylsuccinate from the reaction medium. The obtained erythromycin ethylsuccinate corresponds to the Xth edition of Romanian Pharmacopoeia standards. So, the raw materials consumption is decreased, the costs are cut down, the obtained product purity is high and the output of the process is greater.
Stan
Cătălina Daniela
CD
Disciplina de Industria Medicamentului si Biotehnologii Farmaceutice, Facultatea de Farmacie, Universitatea de Medicină si Farmacie Gr.T. Popa Iaşi.
Stefanache
Alina
A
Tântaru
Gladiola
G
Poiată
Antonia
A
Dumitrache
M
M
Diaconu
D E
DE
Profire
Lenuţa
L
D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000191:economics / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D003460:Crystallization / Q000191:economics; D015643:Erythromycin Ethylsuccinate / Q000138:chemical synthesis* / Q000191:economics / Q000494:pharmacology*; D006801:Humans; D016982:Micrococcus luteus / Q000187:drug effects*; D014867:Water
D000900:Anti-Bacterial Agents; D014867:Water; D015643:Erythromycin Ethylsuccinate