Effect of nitroethane, dimethyl-2-nitroglutarate and 2-nitro-methyl-propionate on ruminal methane production and hydrogen balance in vitro.
Ruminal methanogenesis is considered a digestive inefficiency that results in the loss of 2-12% of the host's gross energy intake and contributes nearly 20% to the United States annual CH(4) emissions. Presently, the effects of the known CH(4) inhibitor, nitroethane, and two synthetic nitrocompounds, dimethyl-2-nitroglutarate and 2-nitro-methyl-propionate, on ruminal CH(4) production and fermentation were evaluated in vitro. After 24 h incubation at 39 degrees C under 100% CO(2), ruminal fluid cultures treated with 2.97 or 11.88 mumol ml(-1) of the respective nitrocompounds produced > 92% less CH(4) (P < 0.05) than non-treated controls. Quantification of fermentation end-products produced and H(2) balance estimates indicate that fermentation efficiencies were not compromised by the nitro-treatments.
Anderson
Robin C
RC
United States Department of Agriculture/Agricultural Research Service, Southern Plains Agricultural Research Center, Food & Feed Safety Research Unit, 2881 F&B Road, College Station, Texas 77845 USA. Robin.Anderson@ars.usda.gov
Huwe
Janice K
JK
Smith
David J
DJ
Stanton
Thaddeus B
TB
Krueger
Nathan A
NA
Callaway
Todd R
TR
Edrington
Thomas S
TS
Harvey
Roger B
RB
Nisbet
David J
DJ
D000818:Animals; D002245:Carbon Dioxide / Q000737:chemistry; D002417:Cattle; D004980:Ethane / Q000031:analogs & derivatives* / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005285:Fermentation; D005740:Gases; D005977:Glutarates / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D066298:In Vitro Techniques; D000455:Medicago sativa / Q000378:metabolism; D008697:Methane / Q000737:chemistry*; D017672:Nitrogen Compounds / Q000737:chemistry; D009595:Nitroparaffins / Q000737:chemistry*; D011422:Propionates / Q000737:chemistry*; D012418:Ruminants; D013696:Temperature
D005227:Fatty Acids; D005740:Gases; D005977:Glutarates; D017672:Nitrogen Compounds; D009595:Nitroparaffins; D011422:Propionates; C553165:dimethyl 2-nitroglutarate; D002245:Carbon Dioxide; C014705:nitroethane; D006859:Hydrogen; D004980:Ethane; D008697:Methane