Crystal structure of PsbQ from Synechocystis sp. PCC 6803 at 1.8 A: implications for binding and function in cyanobacterial photosystem II.
In Synechocystis sp. PCC 6803, PsbQ is associated with photosystem II (PSII) complexes with the highest activity and stability. However, this subunit is not found in PSII X-ray crystallographic structures from Thermosynechococcus elongatus or Thermosynechococcus vulcanus. We present the crystal structure of cyanobacterial PsbQ determined in the presence and absence of Zn(2+). The protein has a well-defined helical core, containing four helices arranged in an up-down-up-down fold. A conserved potential interaction site composed of a divalent metal binding site and adjacent hydrophobic pocket has been identified.
Jackson
Simon A
SA
Department of Biochemistry, University of Otago, Dunedin 9054, New Zealand.
Fagerlund
Robert D
RD
Wilbanks
Sigurd M
SM
Eaton-Rye
Julian J
JJ
D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D000458:Cyanobacteria; D045332:Photosystem II Protein Complex / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry; D046939:Synechocystis / Q000737:chemistry*; D015032:Zinc
D001426:Bacterial Proteins; D045332:Photosystem II Protein Complex; D021122:Protein Subunits; D015032:Zinc