title: Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype.
abstract: Isolates of ectomycorrhizal Pisolithus albus were sampled from both ultramafic and volcano-sedimentary soils in New Caledonia, a tropical hotspot of biodiversity, to investigate the relationships between genetic diversity and edaphic constraint through tolerance to nickel (Ni). Carpophore description, spore morphology and phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequences confirmed that all isolates belong to P. albus and are closely related to other Australasian specimens. Using molecular tools, ITS-restriction fragment length polymorphism and amplified fragment length polymorphism markers, we showed the existence of two distinct genetic clusters within P. albus: ultramafic and volcano-sedimentary. Mycelia response to Ni toxicity supports such a population structure. Pisolithus albus from ultramafic soils included isolates with a high diversity of in vitro Ni tolerance, with both Ni-tolerant isolates (average Ni EC(50) at 575 microM) and Ni-sensitive isolates (average Ni EC(50) at 37 microM). In contrast, all isolates from volcano-sedimentary soils were found to be Ni sensitive (average Ni EC(50) at 32 microM). We highlight that (1) P. albus population from ultramafic soils of New Caledonia are genetically structured in ecotype, and that (2) Ni tolerance among ultramafic isolates suggests an adaptive physiological response to Ni toxicity.
authors.affiliation: Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), UMR 113 CIRAD/INRA/IRD/SupAgro/UMII, Centre IRD, Nouméa, Nouvelle-Calédonie. philippe.jourand@ird.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Jourand
authors.forename: Marc
authors.initials: M
authors.lastname: Ducousso
authors.forename: Clarisse
authors.initials: C
authors.lastname: Loulergue-Majorel
authors.forename: Laure
authors.initials: L
authors.lastname: Hannibal
authors.forename: Sylvain
authors.initials: S
authors.lastname: Santoni
authors.forename: Yves
authors.initials: Y
authors.lastname: Prin
authors.forename: Michel
authors.initials: M
authors.lastname: Lebrun
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D014644:Genetic Variation; D025282:Mycelium / Q000378:metabolism; D038821:Mycorrhizae / Q000235:genetics* / Q000302:isolation & purification; D009510:New Caledonia; D009532:Nickel / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012987:Soil; D009532:Nickel