title: Identification of a Z-band associated protein complex involving KY, FLNC and IGFN1.
abstract: The KY protein underlies a form of muscular dystrophy in the mouse but its role in muscle remains elusive. Immunodetection of endogenous KY protein in C2C12-derived myotubes and expression of a recombinant form in neonatal cardiomyocytes indicated that KY is a Z-band associated protein. Moreover, characterization of a KY interacting protein fragment led to the identification of Igfn1 (Immunoglobulin-like and fibronectin type 3 domain containing 1). Igfn1 is a transcriptionally complex locus encoding many protein variants. A yeast two-hybrid screen identified the Z-band protein filamin C (FLNC) as an interacting partner. Consistent with this, expression of an IGFN1 recombinant fragment showed that the three N-terminal globular domains, common to at least five IGFN1 variants, are sufficient to provide Z-band targeting. Taken together, the yeast two-hybrid, biochemical and immunofluorescence data support the notion that KY, IGFN1 and FLNC are part of a Z-band associated protein complex likely to provide structural support to the skeletal muscle sarcomere.
authors.affiliation: MRC Harwell, Harwell OX11 0RD, UK.
authors.forename: Jane
authors.initials: J
authors.lastname: Baker
authors.forename: Genna
authors.initials: G
authors.lastname: Riley
authors.forename: M Rosario
authors.initials: MR
authors.lastname: Romero
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Haynes
authors.forename: Helen
authors.initials: H
authors.lastname: Hilton
authors.forename: Michelle
authors.initials: M
authors.lastname: Simon
authors.forename: John
authors.initials: J
authors.lastname: Hancock
authors.forename: Hilda
authors.initials: H
authors.lastname: Tateossian
authors.forename: Vera M
authors.initials: VM
authors.lastname: Ripoll
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Blanco
mesh_terms: D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002460:Cell Line; D003285:Contractile Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D064448:Filamins; D066298:In Vitro Techniques; D051379:Mice; D008840:Microfilament Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D046912:Multiprotein Complexes / Q000737:chemistry / Q000302:isolation & purification; D018485:Muscle Fibers, Skeletal / Q000737:chemistry; D009124:Muscle Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018482:Muscle, Skeletal / Q000737:chemistry; D032383:Myocytes, Cardiac / Q000378:metabolism; D010447:Peptide Hydrolases; D025941:Protein Interaction Mapping; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012518:Sarcomeres / Q000737:chemistry; D014018:Tissue Distribution; D020798:Two-Hybrid System Techniques
chemical_list: D002352:Carrier Proteins; D003285:Contractile Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D064448:Filamins; D008840:Microfilament Proteins; D046912:Multiprotein Complexes; D009124:Muscle Proteins; D011994:Recombinant Proteins; C506764:KY protein, mouse; D010447:Peptide Hydrolases