[Evaluation of percentage of lymphocytes B with expression of co-receptors CD 40, CD22 and CD72 in hypertrophied adenoid at children with otitis media with effusion].
BACKGROUND
In hypertrophied adenoid lymphocytes B make up about 60% all lymphocytes. When the lymphocytes B come in interaction with antigens this membranes signal be passed through their receptor (BCR) to interior of cell. This signal affect modulation on gene expression, activation from which depends activation, anergy or apoptosis of lymphocyte B. Accompany BCR co-receptors regulate his functions influence stimulate or inhibitive. To the most important co-receptors stepping out on lymphocyte B belong: CD40, CD22, CD72.


OBJECTIVE
The aim of study was evaluation of lymphocytes B (CD19) with co-expression with CD72 and CD40 receptors in hypertrophied adenoid with at children with otitis media with effusion.


METHODS
An investigation was executed in hypertrophied adenoids with or without otitis media with effusion.


METHODS
By flow cytometry percentage of lymphocytes B with co-receptors CD 40, CD22 and CD72 in was analyzed.


RESULTS
The percentages of CD19+CD72+ lymphocytes in the group of children with adenoid hypertrophy and exudative otitis media were lower as compared to the reference group. However, the percentages of CD19+CD22+, CD19+CD40+ in the study group was approximate to the reference group.


CONCLUSIONS
The lower percentage of lymphocytes B CD72 + near approximate percentages of lymphocytes B CD40+ and BCD22+ at children with otitis media with effusion can be the cause of incorrect humoral response in hypertrophied adenoid at children. Maybe it is cause reduced spontaneous production IgA and IgG through lymphocyte at children with otitis media with effusion.
Wysocka
Jolanta
J
Zakad Laboratoryjnej Diagnostyki Pediatrycznej Uniwersytetu Medycznego w Białymstoku.
Zelazowska-Rutkowska
Beata
B
Ratomski
Karol
K
Skotnicka
Bozena
B
Hassmann-Poznańska
Elzbieta
E
D000234:Adenoids / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D000293:Adolescent; D015703:Antigens, CD / Q000378:metabolism*; D018941:Antigens, CD19 / Q000378:metabolism*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology*; D015415:Biomarkers / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D006984:Hypertrophy / Q000276:immunology; D008297:Male; D010034:Otitis Media with Effusion / Q000276:immunology* / Q000601:surgery; D012720:Severity of Illness Index; D051918:Sialic Acid Binding Ig-like Lectin 2 / Q000378:metabolism*; D018709:Statistics, Nonparametric
D015703:Antigens, CD; D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte; D015415:Biomarkers; D019013:CD40 Antigens; C064228:CD72 protein, human; D051918:Sialic Acid Binding Ig-like Lectin 2