title: Production optimization and properties of beta glucosidases from a marine fungus Aspergillus-SA 58.
abstract: Aspergillus strain SA 58, showing considerable beta glucosidase production was selected as the potential strain. The fungus showed enzyme production in both acidic and alkaline pH. A temperature of 35 degrees C was found to be optimum for enzyme production. Maximum enzyme production was seen when pectin was used as the carbon source (80 U/ml). In solid-state fermentation, an enzyme production of 6200 U/g Initial Dry Substrate was noted. The strain produced two extra cellular enzymes and two intra cellular enzymes. For both the extra cellular enzymes (BGL A and BGL B), 60 degrees C was found to be optimum temperature for activity. BGL A showed an optimum pH of 4.0 while BGL B showed an optimum pH of 3.0 for activity. Both the enzymes showed a second peak of activity at pH 9.0. Both BGL A and BGL B showed high thermal stabilities with residual activities of 86% and 85% even after 6h of incubation at 50 degrees C.
authors.affiliation: Immuno Technology Laboratory, Department of Biotechnology, Cochin, University of Science and Technology, Cochin 682022, India.
authors.forename: K K
authors.initials: KK
authors.lastname: Elyas
authors.forename: Abraham
authors.initials: A
authors.lastname: Mathew
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Sukumaran
authors.forename: P P Manzur
authors.initials: PP
authors.lastname: Ali
authors.forename: K
authors.initials: K
authors.lastname: Sapna
authors.forename: S Ramesh
authors.initials: SR
authors.lastname: Kumar
authors.forename: K R Rekha
authors.initials: KR
authors.lastname: Mol
mesh_terms: D001230:Aspergillus / Q000187:drug effects / Q000201:enzymology*; D002244:Carbon / Q000494:pharmacology; D044602:Cellulases / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability / Q000187:drug effects; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D009584:Nitrogen / Q000494:pharmacology; D010368:Pectins / Q000494:pharmacology; D012623:Seawater / Q000382:microbiology*; D013696:Temperature
chemical_list: D002244:Carbon; D010368:Pectins; D044602:Cellulases; D009584:Nitrogen