title: PCR multiplex for detection of Salmonella Enteritidis, Typhi and Typhimurium and occurrence in poultry meat.
abstract: The occurrence of foodborne diseases is increasing throughout the world. Bacteria of the genus Salmonella are responsible for food poisoning and, in some cases, may be fatal. The aim of this study was to adapt the multiplex PCR technique (mPCR) on the rapid and direct identification of the presence of Salmonella sp. as well as serotypes Enteritidis, Typhi and Typhimurium in poultry carcasses (n=127) and viscera (n=73). The implementation of the standard technique using positive controls was successfully adapted. The results of Salmonella sp. detection in refrigerated viscera showed that the mPCR was able to detect Salmonella genus in 2.74% of these samples. Traditional microbiological analysis also identified the same positive samples for Salmonella sp. but was not able to differentiate the serotype. The serotype Enteritidis was detected by mPCR in 1.37% of the samples. Our conclusion was that the mPCR was able to detect the presence of these bacteria in a short period of time and enabled the identification of serotype Enteritidis in one of the samples found positive for Salmonella sp.
authors.affiliation: The Animal Health Programme, Faculty of Veterinary Medicine - University of Brasília, Brazil.
authors.forename: Camila Guimarães
authors.initials: CG
authors.lastname: de Freitas
authors.forename: Angela Patrícia
authors.initials: AP
authors.lastname: Santana
authors.forename: Patrícia Helena Caldeira
authors.initials: PH
authors.lastname: da Silva
authors.forename: Vítor Salvador Picão
authors.initials: VS
authors.lastname: Gonçalves
authors.forename: Márcia de Aguiar Ferreira
authors.initials: Mde A
authors.lastname: Barros
authors.forename: Fernando Araripe Gonçalves
authors.initials: FA
authors.lastname: Torres
authors.forename: Luci Sayori
authors.initials: LS
authors.lastname: Murata
authors.forename: Simone
authors.initials: S
authors.lastname: Perecmanis
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011200:Poultry / Q000382:microbiology*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D012478:Salmonella Food Poisoning / Q000517:prevention & control; D012477:Salmonella enteritidis / Q000235:genetics / Q000302:isolation & purification; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000235:genetics / Q000302:isolation & purification; D012703:Serotyping / Q000379:methods
chemical_list: D004269:DNA, Bacterial