title: [Effect on factors of energy metabolism in rats skeletal muscle by cold traditional Chinese medicine].
abstract: OBJECTIVE
  To investigate the effect of six cold traditional Chinese medicine on the energy metabolism factors in rats skeletal muscle.
  
  
  METHODS
  The activity of Na(+)-K(+) -ATPase, Ca(2+) -ATPase and succinate dehydrogenase (SDH), the content of muscle glycogen, and the mRNA expression of skeletal muscle uncoupling protein 3 (UCP3) were measured after rats having been administrated with water extracts of Radix Sophorae Flavescentis, Fructus Gardeniae, Cortex Phellodendri, Radix Scutellariae, Rhizoma Coptidis, and Radix Gentianae respectively at the dose of 6.0, 7.0, 8.4, 6.0, 7.0, 4.0 g x kg(-1) for 30 days.
  
  
  RESULTS
  The activity of Na(+) -K(+) -ATPase has been depressed significantly and the content of skeletal muscle glycogen has been increased remarkably by six cold traditional Chinese medicine. The decreased tendency has been found on activity of Ca(2+) -ATPase and SDH, only the Radix scutellariaeg group decreased the Ca(2+) -ATPase activity significantly (P < 0.05), the SDH activity was decreased high significantly by Radix scutellariae, Cortex Phellodendri, Radix Gentianae and significantly by Rhizoma Coptidis. The mRNA expression of UCP3 has been decreased high significantly by all five cold traditional Chinese medicine except Cortex Phellodendri group with the decreased tendency of UCP3 mRNA expression.
  
  
  CONCLUSIONS
  The cold traditional Chinese medicine has the significant effects on the skeletal muscle energy metabolism by decreasing the utlization of the glucose and the activity of mitochondria SDH to reduce the production of ATP, and depressing the activity of Na(+)-K(+) -ATPase and Ca(2+) -ATPase to cut down the consumption of ATP, by decreasing the mRNA expression of UCP3 to decrease the heat production.
authors.affiliation: Animal Science College of Zhejiang University, Hangzhou 310029, China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Peng
authors.forename: Liping
authors.initials: L
authors.lastname: Huang
authors.forename: Xiaoheng
authors.initials: X
authors.lastname: Gao
authors.forename: Su
authors.initials: S
authors.lastname: Zhang
authors.forename: Riyue
authors.initials: R
authors.lastname: Yu
authors.forename: Hongning
authors.initials: H
authors.lastname: Liu
authors.forename: Ningying
authors.initials: N
authors.lastname: Xu
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal