title: Surface protein composition and concentration of whey protein isolate-stabilized oil-in-water emulsions: effect of heat treatment.
abstract: The heat-induced changes in the surface protein compositions and concentrations of whey protein isolate (WPI)-stabilized emulsions were examined. With increasing heating temperature, the amount of adsorbed beta-lactoglobulin (beta-lg) increased whereas the amount of adsorbed alpha-lactalbumin (alpha-la) decreased. In emulsions formed with 0.5 wt% WPI, the adsorbed alpha-la almost disappeared from the interface of the emulsion droplets after heating at 121 degrees C. With heat treatment at 90 degrees C, adsorbed beta-lg increased but adsorbed alpha-la decreased in the first 5 min, and then changed slightly with increasing heating time. This change in the proportions of beta-lg and alpha-la at the interface may have arisen from the displacement of alpha-la from the interface by beta-lg in the aqueous phase. The addition of NaCl resulted in an increase in the total surface protein concentration during heat treatment, but the competition between beta-lg and alpha-la at high temperatures was eliminated. The emulsions lost stability because bridging flocculation of the emulsion droplets occurred during heat treatment in the presence of NaCl (>50 mM).
authors.affiliation: Riddet Institute, Massey University, Palmerston North 4442, New Zealand. a.m.ye@massey.ac.nz
authors.forename: Aiqian
authors.initials: A
authors.lastname: Ye
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D004655:Emulsions; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008565:Membrane Proteins / Q000737:chemistry*; D018613:Microscopy, Confocal; D008894:Milk Proteins / Q000302:isolation & purification*; D009821:Oils / Q000737:chemistry*; D012965:Sodium Chloride / Q000494:pharmacology; D014867:Water / Q000737:chemistry*; D000067816:Whey Proteins
chemical_list: D004655:Emulsions; D008565:Membrane Proteins; D008894:Milk Proteins; D009821:Oils; D000067816:Whey Proteins; D014867:Water; D012965:Sodium Chloride