title: Polyester copolymer scaffolds enhance expression of bone markers in osteoblast-like cells.
abstract: In tissue engineering, the resorbable aliphatic polyester poly(L-lactide) (PLLA) is used as scaffolds in bone regeneration. Copolymers of poly(L-lactide)-co-(epsilon-caprolactone) [poly(LLA-co-CL)] and poly(L-lactide)-co-(1,5-dioxepan-2-one) [poly(LLA-co-DXO)], with superior mechanical properties to PLLA, have been developed to be used as scaffolds, but the influence on the osteogenic potential is unclear. This in vitro study of test scaffolds of poly(LLA-co-CL) and poly(LLA-co-DXO) using PLLA scaffolds as a control demonstrates the attachment and proliferation of human osteoblast-like cells (HOB) as measured by SEM and a methylthiazol tetrazolium (MTT) colorimetric assay, and the progression of HOB osteogenesis for up to 3 weeks; expressed as synthesis of the osteoblast differentiation markers: collagen type 1 (Col 1), alkaline phosphatase, bone sialoprotein, osteocalcin (OC), osteopontin and runt related gene 2 (Runx2). Surface analysis disclosed excellent surface attachment, spread and penetration of the cells into the pores of the test scaffolds compared to the PLLA. MTT results indicated that test scaffolds enhanced the proliferation of HOBs. Cells grown on the test scaffolds demonstrated higher synthesis of Col 1 and OC and also increased bone markers mRNA expression. Compared to scaffolds of PLLA, the poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds enhanced attachment, proliferation, and expression of osteogenic markers by HOBs in vitro. Therefore, these scaffolds might be appropriate carriers for bone engineering.
authors.affiliation: Department of Clinical Dentistry, Center for Clinical Dental Research, Faculty of Medicine and Dentistry, University of Bergen, Bergen 5009, Norway.
authors.forename: Shaza Bushra
authors.initials: SB
authors.lastname: Idris
authors.forename: Kristina
authors.initials: K
authors.lastname: Arvidson
authors.forename: Peter
authors.initials: P
authors.lastname: Plikk
authors.forename: Salah
authors.initials: S
authors.lastname: Ibrahim
authors.forename: Anna
authors.initials: A
authors.lastname: Finne-Wistrand
authors.forename: Ann-Christine
authors.initials: AC
authors.lastname: Albertsson
authors.forename: Anne Isine
authors.initials: AI
authors.lastname: Bolstad
authors.forename: Kamal
authors.initials: K
authors.lastname: Mustafa
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D001861:Bone Regeneration / Q000502:physiology*; D001842:Bone and Bones / Q000502:physiology*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D011091:Polyesters; D012333:RNA, Messenger; C556909:poly(L-lactide)-co-(1,5-dioxepan-2-one); C033616:poly(lactide)