title: [Study on the extraction technology of polysaccharides from Caulis Marsdeniae Tenacissimae].
abstract: OBJECTIVE
  To optimize the extraction technology and to establish the determination method of polysaccharides from Caulis Marsdeniae Tenacissimae.
  
  
  METHODS
  Phenol-sulphate colorimetry was used to determine the content of polysaccharides. According to the yield of polysaccharides, the orthogonal design was used to select the extraction technology of polysaccharides.
  
  
  RESULTS
  The optimum extraction procedure was 8 folds of water, refluxing and extracting for 3 times at 100 degrees C, and 1 h for each time. The concentration of polysaccharide was linear in the range of 0.004-0.014 mg/mL The average recovery was 99.72%, RSD = 0. 63%.
  
  
  CONCLUSIONS
  The optimum extraction procedure is simple and reliable which is suitable for industrial production. The method for determination is simple, specific and accurate which can provide a method for mass control of Marsdenia tenacissima.
authors.affiliation: College of Pharmacognosy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. eleven_lb@126.com
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Xin-yun
authors.initials: XY
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Ting-guo
authors.initials: TG
authors.lastname: Kang
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D029062:Apocynaceae / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014867:Water
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D012997:Solvents; D014867:Water