Complete nucleotide sequence and comparative analysis of pPR9, a 41.7-kilobase conjugative staphylococcal multiresistance plasmid conferring high-level mupirocin resistance.
We have sequenced the conjugative plasmid pPR9, which carries the ileS2 gene, which had contributed to the dissemination of high-level mupirocin resistance at our institution. The plasmid backbone shows extensive genetic conservation with plasmids belonging to the pSK41/pGO1 family, but comparative analyses have revealed key differences that provide important insights into the evolution of these medically important plasmids and high-level mupirocin resistance in staphylococci and highlight the role of insertion sequence IS257 in these processes.
Pérez-Roth
Eduardo
E
Hospital Universitario Nuestra Señora de Candelaria, Departamento de Microbiología y Biología Celular, Universidad de La Laguna, La Laguna, Spain. eperroth@ull.es
Kwong
Stephen M
SM
Alcoba-Florez
Julia
J
Firth
Neville
N
Méndez-Alvarez
Sebastián
S
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D024881:Drug Resistance, Bacterial / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics*; D018740:Genetic Heterogeneity; D016712:Mupirocin / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*
D000900:Anti-Bacterial Agents; D016712:Mupirocin