PHBV and predifferentiated human adipose-derived stem cells for cartilage tissue engineering.
This study was conducted to investigate whether in vitro chondrogenic differentiated human adipose-derived stem cells (hASCs) can maintain the chondrogenic phenotype in (3-hydroxybutrate-co-3-hydroxyvalerate) (PHBV) scaffolds and whether differentiated hASCs/PHBV construct can produce neocartilage in a heterotopic animal model. hASCs were cultured with or without chondrogenic media in vitro and then seeded on PHBV foams. Differentiated cell/PHBV constructs were subcutaneously implanted in nude mice for 8 or 16 weeks; nondifferentiated cell/PHBV constructs were implanted in the control group. The results in the control group showed no cartilage formation and the disappearance of the scaffold at 8 weeks. Conversely, all differentiated hASCs/PHBV implants kept their original shape throughout 16 weeks. These implants at 16 weeks had stronger chondrocytes-specific histochemical staining than those at 8 weeks, with GAG, total collagen, and compressive moduli increased with implantation time. Cartilage lacunae were observed in all retrieved implants at 16 weeks. The chondrocytes-specific genes were detected by RT-PCR at 16 weeks. The remnants of PHBV were observed in the implants throughout 16 weeks. This study demonstrates that chondrogenic predifferentiated hASCs have the ability to maintain a chondrogenic phenotype in PHBV and that cell/PHBV constructs can produce neocartilage in a heterotopic site, but the degradation rates of PHBV in different environments needs more investigation.
Liu
Jiong
J
Department of Orthopaedics, The Fourth People's Hospital of Guiyang, Guiyang 550003, China.
Zhao
Bin
B
Zhang
Yunqiang
Y
Lin
Yunfeng
Y
Hu
Ping
P
Ye
Chuan
C
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002356:Cartilage / Q000166:cytology / Q000502:physiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D020219:Chondrogenesis / Q000502:physiology; D004247:DNA / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D020007:Implants, Experimental; D008297:Male; D008422:Materials Testing; D051379:Mice; D008819:Mice, Nude; D010641:Phenotype; D011091:Polyesters / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D000094122:Polyhydroxybutyrates
D001672:Biocompatible Materials; D004247:DNA; D006025:Glycosaminoglycans; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates