title: In vitro selection of RNA aptamers to a small molecule target.
abstract: This unit describes the selection of aptamers from a single-stranded RNA pool that bind to small molecule targets. Aptamers generated by this type of selection experiment can potentially function as receptors for small molecules in numerous applications, including medical diagnostics, therapeutics, and environmental monitoring. This unit describes two modes of selection, one by column filtration and one by batch selection.
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas, Austin, Texas, USA.
authors.forename: Vlad
authors.initials: V
authors.lastname: Codrea
authors.forename: Michelle
authors.initials: M
authors.lastname: Hayner
authors.forename: Bradley
authors.initials: B
authors.lastname: Hall
authors.forename: Sulay
authors.initials: S
authors.lastname: Jhaveri
authors.forename: Andrew
authors.initials: A
authors.lastname: Ellington
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005374:Filtration; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D012313:RNA