Hsa-miR-34c suppresses growth and invasion of human laryngeal carcinoma cells via targeting c-Met.
MicroRNAs (miRNAs) are small noncoding RNA molecules that negatively modulate gene expression at the post-transcriptional level. A growing number of studies has shown that more and more miRNAs are aberrantly expressed and involved in the pathogenesis of several types of cancers. Here, we report that the down-regulated hsa-miR-34c was also involved in oncogenesis of laryngeal carcinoma. Our studies indicated that hsa-miR-34c functioned as a tumor suppressor which inhibited growth and invasion of human laryngeal carcinoma cells. Furthermore, in our study, an inverse relationship between the expression of hsa-miR-34c and c-Met was identified in 10 paired fresh samples from tumor tissues and adjacent normal tissues. Infection of hsa-miR-34c mediated by lentivirus suppressed the expression of c-Met directly. In addition, introduction of c-Met cDNA lacking 3'-UTR largely abrogated hsa-miR-34c-induced cell growth and invasion inhibition. These findings suggest aberrantly down-regulated hsa-miR-34c is a critical factor that contributes to malignancy in human laryngeal carcinoma by a mechanism involving targeting of c-Met.
Cai
Ke-Min
KM
Department of Otorhinolaryngology Head and Neck Surgery, Taizhou People's Hospital, Taizhou 225300, Jiangsu, P.R. China. njmucaikemin@126.com
Bao
Xue-Li
XL
Kong
Xu-Hui
XH
Jinag
Wu
W
Mao
Ming-Rong
MR
Chu
Jiu-Sheng
JS
Huang
Yong-Jiu
YJ
Zhao
Xiao-Jun
XJ
D020413:3' Untranslated Regions / Q000235:genetics; D017209:Apoptosis; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007822:Laryngeal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics / Q000378:metabolism*
D020413:3' Untranslated Regions; D018076:DNA, Complementary; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D019859:Proto-Oncogene Proteins c-met