Green hydrophilic interaction chromatography using ethanol-water-carbon dioxide mixtures.
In hydrophilic interaction chromatography (HILIC), best results are obtained with high concentrations of acetonitrile. In the framework of green chromatography, different concentrations of carbon dioxide were added to the mobile phases acetonitrile-water and ethanol-water and the impact on retention and separation in HILIC using bare silica as stationary phase was explored. The features of HILIC using enhanced-fluidity mobile phases are illustrated with the analysis of the nucleobases and a mixture containing the nucleobases and cortisol, flurbiprofen, theophylline and caffeine. For both organic constituents, the elution window is widened in function of the carbon dioxide concentration and selectivity changes. At high concentrations of carbon dioxide in ethanol, separations were similar to those obtained with acetonitrile without carbon dioxide addition.
Pereira
Alberto dos Santos
Ados S
Research Institute for Chromatography, Kortrijk, Belgium.
Girón
Ana Jiménez
AJ
Admasu
Engdawork
E
Sandra
Pat
P
D002245:Carbon Dioxide / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D000431:Ethanol / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D014867:Water; D002245:Carbon Dioxide; D000431:Ethanol