title: Wound healing and protease inhibition activity of Bacoside-A, isolated from Bacopa monnieri wettest.
abstract: Bacopa monnieri (L.) Wettest. (Scrophulariaceae) is a well-known medicinal herb. In the Indian system of medicine it is known as Brahmi (Sanskrit) and Indian water hyssop. Methanolic extract of Bacopa monnieri and its isolated constituent Bacoside-A were screened for wound healing activity. Bacoside-A was screened for wound healing activity by excision, incision and dead space wound on Swiss albino rats. Significant wound healing activity was observed in both extract and the Bacoside-A treated groups. The SDS-PAGE caseinolytic zymogram analysis of inhibition of matrix metalloproteases (MMPs) enzyme from the excision wound by Bacoside-A, an isolated constituent, was done with the concentrations 100 and 200 micromg/ml. In Bacoside-A treated groups, epithelialization of the excision wound was faster with a high rate (18.30 +/- 0.01 days) of wound contraction. The tensile strength of the incision wound was increased (538.47 +/- 0.14 g) in the Bacoside-A treated group. In the dead space wound model, the weight of the granuloma was also increased (89.15 +/- 0.08 g). The histological examination of the granuloma tissue of the Bacoside-A treated group showed increased cross-linking of collagen fibers and absence of monocytes. The wound healing activity of Bacoside-A was more effective in various wound models compared to the standard skin ointment Nitrofurazone.
authors.affiliation: PG Department and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta, Karnataka, India.
authors.forename: R
authors.initials: R
authors.lastname: Sharath
authors.forename: B G
authors.initials: BG
authors.lastname: Harish
authors.forename: V
authors.initials: V
authors.lastname: Krishna
authors.forename: B N
authors.initials: BN
authors.lastname: Sathyanarayana
authors.forename: H M Kumara
authors.initials: HM
authors.lastname: Swamy
mesh_terms: D000279:Administration, Cutaneous; D000284:Administration, Oral; D000818:Animals; D034681:Bacopa / Q000737:chemistry*; D006099:Granuloma / Q000473:pathology; D061965:Matrix Metalloproteinase Inhibitors; D009583:Nitrofurazone / Q000627:therapeutic use; D010936:Plant Extracts / Q000627:therapeutic use*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D012503:Saponins / Q000627:therapeutic use*; D014315:Triterpenes / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C500175:bacoside A; D009583:Nitrofurazone