title: Negative regulation of Wnt signaling mediated by CK1-phosphorylated Dishevelled via Ror2.
abstract: Dishevelled (Dvl) is a multifunctional effector of different Wnt cascades. Both canonical Wnt3a and noncanonical Wnt5a stimulate casein-kinase-1 (CK1) -mediated phosphorylation of Dvl, visualized as electrophoretic mobility shift [phosphorylated and shifted Dvl (ps-Dvl)]. However, the role of this phosphorylation remains obscure. Here we report the functional interaction of ps-Dvl with the receptor tyrosine kinase Ror2, which is an alternative Wnt receptor and is able to inhibit canonical Wnt signaling. We demonstrate interaction between Ror2 and ps-Dvl at the cell membrane after Wnt3a or Wnt5a stimulus dependent on CK1. Ps-Dvl interacts with the C-terminal proline-serine-threonine-rich domain of Ror2, which is required for efficient inhibition of canonical Wnt signaling. We further show that the Dvl C terminus, which seems to be exposed in ps-Dvl and efficiently binds Ror2, is an intrinsic negative regulator of the canonical Wnt pathway downstream of beta-catenin. The Dvl C terminus is necessary and sufficient to inhibit canonical Wnt/beta-catenin signaling, which is dependent on the presence of Ror2. Furthermore, both the Dvl C terminus and CK1epsilon can inhibit the Wnt5a/Ror2/ATF2 pathway in mammalian cells and Xenopus explant cultures. This suggests that phosphorylation of Dvl triggers negative feedback regulation for different branches of Wnt signaling in a Ror2-dependent manner.
authors.affiliation: Development and Disease Group, Max Planck Institute for Molecular Genetics, Ihnestr. 73, 14195 Berlin, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Witte
authors.forename: Ondrej
authors.initials: O
authors.lastname: Bernatik
authors.forename: Katharina
authors.initials: K
authors.lastname: Kirchner
authors.forename: Jan
authors.initials: J
authors.lastname: Masek
authors.forename: Annika
authors.initials: A
authors.lastname: Mahl
authors.forename: Pavel
authors.initials: P
authors.lastname: Krejci
authors.forename: Stefan
authors.initials: S
authors.lastname: Mundlos
authors.forename: Alexandra
authors.initials: A
authors.lastname: Schambony
authors.forename: Vitezslav
authors.initials: V
authors.lastname: Bryja
authors.forename: Sigmar
authors.initials: S
authors.lastname: Stricker
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D019556:COS Cells; D047389:Casein Kinase I / Q000378:metabolism*; D002522:Chlorocebus aethiops; D000072261:Dishevelled Proteins; D025461:Feedback, Physiological; D006801:Humans; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D057050:Receptor Tyrosine Kinase-like Orphan Receptors / Q000378:metabolism*; D015398:Signal Transduction; D051153:Wnt Proteins / Q000378:metabolism*; D000071818:Wnt-5a Protein; D060508:Wnt3 Protein; D060509:Wnt3A Protein; D014981:Xenopus; D029867:Xenopus Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C000606586:DVL1 protein, Xenopus; D000072261:Dishevelled Proteins; D010750:Phosphoproteins; C555506:WNT3A protein, Xenopus; C555510:WNT3A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; D060508:Wnt3 Protein; D060509:Wnt3A Protein; C555511:Wnt3a protein, mouse; C438615:Wnt5a protein, mouse; D029867:Xenopus Proteins; D057050:Receptor Tyrosine Kinase-like Orphan Receptors; D047389:Casein Kinase I