Regulation of leukocyte recruitment by the long pentraxin PTX3.
Pentraxins are a superfamily of conserved proteins involved in the acute-phase response and innate immunity. Pentraxin 3 (PTX3), a prototypical member of the long pentraxin subfamily, is a key component of the humoral arm of innate immunity that is essential for resistance to certain pathogens. A regulatory role for pentraxins in inflammation has long been recognized, but the underlying mechanisms remain unclear. Here we report that PTX3 bound P-selectin and attenuated neutrophil recruitment at sites of inflammation. PTX3 released from activated leukocytes functioned locally to dampen neutrophil recruitment and regulate inflammation. Antibodies have glycosylation-dependent regulatory effect on inflammation. Therefore, PTX3, which is an essential component of humoral innate immunity, and immunoglobulins share functional outputs, including complement activation, opsonization and, as shown here, glycosylation-dependent regulation of inflammation.
Deban
Livija
L
Laboratory for Immunology and Inflammation, Instituto di Ricovero e Cura a Carattere Scientifico-Istituto Clinico Humanitas, Rozzano, Italy.
Russo
Remo Castro
RC
Sironi
Marina
M
Moalli
Federica
F
Scanziani
Margherita
M
Zambelli
Vanessa
V
Cuccovillo
Ivan
I
Bastone
Antonio
A
Gobbi
Marco
M
Valentino
Sonia
S
Doni
Andrea
A
Garlanda
Cecilia
C
Danese
Silvio
S
Salvatori
Giovanni
G
Sassano
Marica
M
Evangelista
Virgilio
V
Rossi
Barbara
B
Zenaro
Elena
E
Constantin
Gabriela
G
Laudanna
Carlo
C
Bottazzi
Barbara
B
Mantovani
Alberto
A
D055371:Acute Lung Injury / Q000276:immunology; D000818:Animals; D002097:C-Reactive Protein / Q000276:immunology*; D016466:CHO Cells; D002469:Cell Separation; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology; D007249:Inflammation / Q000276:immunology*; D036904:Leukocyte Rolling / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020556:Neutrophil Infiltration / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D000683:Serum Amyloid P-Component / Q000276:immunology*; D000099153:Pentraxins
D002097:C-Reactive Protein; D011994:Recombinant Proteins; D000683:Serum Amyloid P-Component; D000099153:Pentraxins