title: Production and characterization of monoclonal antibodies to mouse germ cells.
abstract: In mammals, primordial germ cells (PGCs) are generated in the extra-embryonic epiblast, and thereafter migrate into the developing gonads. Following the development of the gonads to the testes or ovaries, germ cells mature into sperms or eggs. In the present study, we report production and characterization of monoclonal antibodies (MAb) that recognize PGCs. Extracts from E12.5 mouse embryonic gonads were immunized as an antigen, and hybridomas were generated using the rat medial iliac lymph node method. The hybridoma supernatants were screened by immunohistochemical analyses of E12.5 mouse embryonic sections. The antibody, referred to herein as MAb 5B5, provided strong signals on PGCs. Moreover, immunofluorescence analyses using a variety of the tissue sections of mouse embryos revealed that MAb 5B5 also recognizes the ventricular zone of the cerebral cortex and the neural canal in the spinal cord in which neural specific stem cells are present in abundance. Based on these findings, MAb 5B5 might recognize stem cell-associated antigens.
authors.affiliation: Department of Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto, Sumiyoshi-ku, Osaka, Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Yokoyama
authors.forename: Yuko
authors.initials: Y
authors.lastname: Katoh-Fukui
authors.forename: Ken-Ichirou
authors.initials: K
authors.lastname: Morohashi
authors.forename: Daijiro
authors.initials: D
authors.lastname: Konno
authors.forename: Masayuki
authors.initials: M
authors.lastname: Azuma
authors.forename: Taro
authors.initials: T
authors.lastname: Tachibana
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D005854:Germ Cells / Q000276:immunology*; D006066:Gonads / Q000196:embryology / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology*; D007082:Ileum / Q000276:immunology; D007114:Immunization; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY; D013234:Stem Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase