title: Isolation and characterization of antineoplastic alkaloids from Catharanthus roseus L. Don. cultivated in Egypt.
abstract: Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus roseus L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid chromatographic column on silica gel : aluminium oxide (1:1) mixed bed vacuum liquid chromatography (VLC), Charcoal column, and finally purified by centrifugally accelerated radial chromatography (Chromatotrone).
authors.affiliation: Phytochemistry Department, National Research Centre, Dokki, Giza, Egypt, 12311. khaledashams@yahoo.com
authors.forename: Khaled A
authors.initials: KA
authors.lastname: Shams
authors.forename: Naglaa M
authors.initials: NM
authors.lastname: Nazif
authors.forename: Nahla S
authors.initials: NS
authors.lastname: Abdel Azim
authors.forename: Khaled A
authors.initials: KA
authors.lastname: Abdel Shafeek
authors.forename: Mostafa M
authors.initials: MM
authors.lastname: El-Missiry
authors.forename: Shams I
authors.initials: SI
authors.lastname: Ismail
authors.forename: Medhat M
authors.initials: MM
authors.lastname: Seif El Nasr
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D030008:Catharanthus / Q000737:chemistry*; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D004534:Egypt; D010936:Plant Extracts / Q000737:chemistry*; D014747:Vinblastine / Q000302:isolation & purification*; D014750:Vincristine / Q000302:isolation & purification*
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D014750:Vincristine; D014747:Vinblastine