title: Evaluation of a collagenase generated osteoarthritis biomarker in the synovial fluid from elbow joints of dogs with medial coronoid disease and unaffected dogs.
abstract: OBJECTIVE
  To evaluate whether synovial fluid concentrations of an osteoarthritis biomarker in dysplastic canine elbows with medial coronoid disease (MCD) are elevated compared with unaffected elbows and to determine if these concentrations correlate to the degree of articular cartilage damage.
  
  
  METHODS
  Cross sectional clinical study.
  
  
  METHODS
  Dogs (n=19; 35 elbows) with MCD and dogs (8; 16 elbows) with unaffected elbows.
  
  
  METHODS
  Concentrations of a collagenase-generated cleavage neoepitope of type II collagen (Col2-3/4C(long mono), or C2C) in joint fluid from elbows were analyzed and compared between dogs with MCD and unaffected dogs. Correlation of C2C concentration with subjective grading of articular cartilage surface damage was also evaluated.
  
  
  RESULTS
  Mean (+/-SD) C2C concentration from MCD dogs was significantly higher (112.3+/-24.8 ng/mL) than in unaffected dogs (76.1+/-16.9 ng/mL; P<.05). There was a moderate correlation between cartilage damage grade and increasing C2C concentrations (P<.05, r=0.62)
  
  
  CONCLUSIONS
  C2C concentrations are elevated in the synovial fluid of dogs with MCD compared with unaffected elbows, and a moderate, significant correlation was identified between these concentrations and subjective grading of articular cartilage damage.
  
  
  CONCLUSIONS
  This preliminary data suggest that C2C concentrations in synovial fluid may have potential as a biomarker for diagnosis of articular cartilage damage associated with MCD and as a means of objectively determining the degree of articular cartilage damage.
authors.affiliation: JD Wheat Veterinary Orthopedic Research Laboratory, the Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616-8745, USA.
authors.forename: Adam
authors.initials: A
authors.lastname: Prink
authors.forename: Kei
authors.initials: K
authors.lastname: Hayashi
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: James
authors.initials: J
authors.lastname: Kim
authors.forename: Amy
authors.initials: A
authors.lastname: Kapatkin
mesh_terms: D000818:Animals; D001182:Arthroscopy / Q000662:veterinary; D015415:Biomarkers / Q000032:analysis*; D002358:Cartilage, Articular / Q000378:metabolism / Q000473:pathology; D017364:Collagenases / Q000032:analysis*; D004283:Dog Diseases / Q000175:diagnosis* / Q000473:pathology; D004285:Dogs; D005260:Female; D005552:Forelimb; D007794:Lameness, Animal / Q000175:diagnosis; D008297:Male; D010003:Osteoarthritis / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D013582:Synovial Fluid / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D017364:Collagenases