Genotypic linkages of VP4, VP6, VP7, NSP4, NSP5 genes of rotaviruses circulating among children with acute gastroenteritis in Thailand.
Rotavirus is the main cause of acute viral gastroenteritis in infants and young children worldwide. Surveillance of group A rotavirus has been conducted in Chiang Mai, Thailand since 1987 up to 2004 and those studies revealed that group A rotavirus was responsible for about 20-61% of diarrheal diseases in hospitalized cases. In this study, we reported the continuing surveillance of group A rotavirus in 2005 and found that group A rotavirus was detected in 43 out of 147 (29.3%) stool samples. Five different G and P genotype combinations were detected, G1P[8] (27 strains), G2P[4] (12 strains), G9P[8] (2 strains), G3P[8] (1 strain), and G3P[10] (1 strain). In addition, analysis of their genotypic linkages of G (VP7), P (VP4), I (VP6), E (NSP4), and H (NSP5) genotypes demonstrated that the rotaviruses circulating in Chiang Mai, Thailand carried 3 unique linkage patterns. The G1P[8], G3P[8], and G9P[8] strains carried their VP6, NSP4, NSP5 genotypes of I1, E1, H1, respectively. The G2P[4] strains were linked with I2, E2, H2 genotypes, while an uncommon G3P[10] genotype carried unique genotypes of I8, E3 and H6. These findings provide the overall picture of genotypic linkage data of rotavirus strains circulating in Chiang Mai, Thailand.
Khamrin
Pattara
P
Aino Health Science Center, Aino University, Tokyo, Japan.
Maneekarn
Niwat
N
Malasao
Rungnapa
R
Nguyen
Tuan Anh
TA
Ishida
Shinichi
S
Okitsu
Shoko
S
Ushijima
Hiroshi
H
D000208:Acute Disease; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics*; D002675:Child, Preschool; D005759:Gastroenteritis / Q000821:virology*; D008040:Genetic Linkage / Q000235:genetics*; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D007223:Infant; D010802:Phylogeny; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000821:virology*; D013785:Thailand; D014118:Toxins, Biological / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics*
D000956:Antigens, Viral; D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; C060339:VP4 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins