title: Immunostimulatory acivity of Calophyllum brasiliense, Ipomoea pes-caprae and Matayba elaeagnoides demonstrated by human peripheral blood mononuclear cells proliferation.
abstract: This study evaluates the effect of methanol extracts of three Brazilian medicinal plants on in vitro proliferation of human mononuclear cells. Lymphoproliferation assay was carried out by incubating human peripheral blood mononuclear cells from healthy donors (1 x 10(6) cells/mL) with extracts of Calophyllum brasiliense (roots), Ipomoea pes-caprae (whole plant) and Matayba elaeagnoides (bark), both at 10, 50, 100 and 200 microg/mL, alone or with phytohemagglutinin (PHA, 5 microg/mL), in 96-well microplates at 37 degrees C with 5% CO2, for 72 h. The quantification of cell proliferation assay was performed by blue tetrazolium (MTT) reduction with reading at 540 nm. Cells incubated with only the culture medium were used as negative control for cell proliferation, while the positive control consisted of cells and PHA. The results suggest that the extracts of all three studied plants induce T lymphocyte proliferation. I. pes-caprae showed immunostimulatory activity three times higher than the C. brasiliense extract, while that of the M. elaeagnoides extract was 1.5 times higher. The results demonstrate immunostimulatory effects of these three plants, therefore the continuity of these studies is recommended, in order to determine the active principles.
authors.affiliation: Programa de Mestrado em Ciências Farmacêuticas and Núcleo de Investigações Químico-Farmacêuticas, Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, bloco 17, Itajaí-SC, 88.302-202, Brazil.
authors.forename: Marina Elisa
authors.initials: ME
authors.lastname: Philippi
authors.forename: Bruna Momm
authors.initials: BM
authors.lastname: Duarte
authors.forename: Carolina Vieira
authors.initials: CV
authors.lastname: Da Silva
authors.forename: Michel Thomaz
authors.initials: MT
authors.lastname: De Souza
authors.forename: Rivaldo
authors.initials: R
authors.lastname: Niero
authors.forename: Valdir
authors.initials: V
authors.lastname: Cechinel Filho
authors.forename: Edneia Casagranda
authors.initials: EC
authors.lastname: Bueno
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D028084:Clusiaceae; D004305:Dose-Response Relationship, Drug; D006801:Humans; D027741:Ipomoea; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D010946:Plants, Medicinal; D029631:Sapindaceae; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D013997:Time Factors
chemical_list: D000276:Adjuvants, Immunologic; D010835:Phytohemagglutinins; D010936:Plant Extracts