title: Recombinant expression in moderate halophiles.
abstract: A novel expression of recombinant proteins was developed using moderate halophiles that accumulate osmolytes and hence provide cytoplasmic environments where osmolyte-driven folding can take place. Promoters and selection marker were developed for high expression of foreign proteins. Examples are given for expression of bacterial nucleoside diphosphate kinase and human serine racemase.
authors.affiliation: Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan. tokunaga@chem.agri.kagoshima-u.ac.jp
authors.forename: Masao
authors.initials: M
authors.lastname: Tokunaga
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Arakawa
authors.forename: Hiroko
authors.initials: H
authors.lastname: Tokunaga
mesh_terms: D024861:Genetic Enhancement / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D016931:Halobacteriales / Q000502:physiology*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins