title: Glycoprotein D of bovine herpesvirus 5 (BoHV-5) confers an extended host range to BoHV-1 but does not contribute to invasion of the brain.
abstract: Bovine herpesvirus 1 (BoHV-1) and BoHV-5 are closely related pathogens of cattle, but only BoHV-5 is considered a neuropathogen. We engineered intertypic gD exchange mutants with BoHV-1 and BoHV-5 backbones in order to address their in vitro and in vivo host ranges, with particular interest in invasion of the brain. The new viruses replicated in cell culture with similar dynamics and to titers comparable to those of their wild-type parents. However, gD of BoHV-5 (gD5) was able to interact with a surprisingly broad range of nectins. In vivo, gD5 provided a virulent phenotype to BoHV-1 in AR129 mice, featuring a high incidence of neurological symptoms and early onset of disease. However, only virus with the BoHV-5 backbone, independent of the gD type, was detected in the brain by immunohistology. Thus, gD of BoHV-5 confers an extended cellular host range to BoHV-1 and may be considered a virulence factor but does not contribute to the invasion of the brain.
authors.affiliation: Institute of Virology, University of Zurich, Winterthurerstrasse 266a, 8057 Zurich, Switzerland.
authors.forename: Evgeni
authors.initials: E
authors.lastname: Gabev
authors.forename: Kurt
authors.initials: K
authors.lastname: Tobler
authors.forename: Carlos
authors.initials: C
authors.lastname: Abril
authors.forename: Monika
authors.initials: M
authors.lastname: Hilbe
authors.forename: Claudia
authors.initials: C
authors.lastname: Senn
authors.forename: Marco
authors.initials: M
authors.lastname: Franchini
authors.forename: Gabriella
authors.initials: G
authors.lastname: Campadelli-Fiume
authors.forename: Cornel
authors.initials: C
authors.lastname: Fraefel
authors.forename: Mathias
authors.initials: M
authors.lastname: Ackermann
mesh_terms: D000818:Animals; D001921:Brain / Q000821:virology*; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000378:metabolism; D007242:Herpesvirus 1, Bovine / Q000472:pathogenicity*; D025881:Herpesvirus 5, Bovine / Q000302:isolation & purification / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions / Q000276:immunology; D051379:Mice; D000075983:Nectins; D009422:Nervous System Diseases / Q000821:virology; D011485:Protein Binding; D014759:Viral Envelope Proteins / Q000502:physiology*
chemical_list: D015815:Cell Adhesion Molecules; D000075983:Nectins; D014759:Viral Envelope Proteins; C091563:glycoprotein D, bovine herpesvirus 5