title: Amelioration of streptozotocin-induced diabetes by Agrocybe chaxingu polysaccharide.
abstract: The aim of this study was to investigate the preventive effect of Agrocybe chaxingu polysaccharide on streptozocin (STZ)-induced pancreatic beta-cells destruction. Agrocybe chaxingu polysaccharide markedly reduced nitric oxide (NO) production and iNOS expression levels in RINm5F cells in a dose-dependent manner. In addition, Agrocybe chaxingu polysaccharide significantly inhibited iNOS expression and blood glucose levels in STZ-induced diabetic mice. Moreover, immunohistochemical analysis revealed that it enhanced pancreatic beta-cells resistance to destruction by STZ. These results suggest that Agrocybe chaxingu polysaccharide may have value as a therapeutic agent against diabetes mellitus.
authors.affiliation: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon, 200-702, Korea.
authors.forename: Byung Ryong
authors.initials: BR
authors.lastname: Lee
authors.forename: Yeom Pyo
authors.initials: YP
authors.lastname: Lee
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Ha Yong
authors.initials: HY
authors.lastname: Song
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Kwang Jae
authors.initials: KJ
authors.lastname: Lee
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Kim
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Joo
authors.forename: Hun Ju
authors.initials: HJ
authors.lastname: Ham
authors.forename: Jang Hyun
authors.initials: JH
authors.lastname: Hur
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Kyu Hyung
authors.initials: KH
authors.lastname: Han
authors.forename: Kil Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D055362:Agrocybe / Q000737:chemistry*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D020030:Nitric Oxide Donors; D011134:Polysaccharides; D009599:Nitroprusside; D009569:Nitric Oxide; D013311:Streptozocin; D052247:Nitric Oxide Synthase Type II