Systemic minocycline differentially influences changes in spinal microglial markers following formalin-induced nociception.
In the present study, intraperitoneal administration of minocycline attenuated enhancing nociceptive behaviors in those rats receiving dual formalin injections (5% formalin followed at 7 days later by 1% formalin). The minocycline treatment did not prevent the increase in OX-42 and MHC class I labeling and morphological changes, but significantly attenuated upregulation of phospho-p38 in activated microglia. These results suggest that the later days of microglial activation with upregulated immune markers in the spinal cord contributes to enhancing long-term pain response by a pathway of p38 activation in microglia.
Li
Kai
K
Center for TMD & Orofacial Pain, Peking University School & Hospital of Stomatology, Beijing, PR China.
Fu
Kai-Yuan
KY
Light
Alan R
AR
Mao
Jianren
J
D000818:Animals; D018949:Antigens, CD1 / Q000378:metabolism; D004195:Disease Models, Animal; D005557:Formaldehyde; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D008297:Male; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D008911:Minocycline / Q000494:pharmacology* / Q000627:therapeutic use*; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology*; D010147:Pain Measurement / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D018949:Antigens, CD1; C419394:CD1b antigen; D015395:Histocompatibility Antigens Class I; D005557:Formaldehyde; D048051:p38 Mitogen-Activated Protein Kinases; D008911:Minocycline