title: Chemical constituents of Alnus firma and their inhibitory activity on lipopolysaccharide-induced nitric oxide production in BV2 microglia.
abstract: A methanolic extract of ALNUS FIRMA barks (Betulaceae) significantly attenuated nitric oxide production in lipopolysaccharide-stimulated BV2 microglia. Two new compounds, characterized as 4-hydroxy-2,6-dimethoxyphenyl 6'- O-syringoyl- beta- D-glucopyranoside (1) and 4-hydroxy-2,6-dimethoxyphenyl 6'- O-vanilloyl- beta- D-glucopyranoside (2), were isolated from the barks of A. FIRMA using bioactivity-guided fractionation, together with two known phenolic glycosides (3, 4) and 11 known diarylheptanoids (5- 15). Among these compounds, 1- 4 and 6- 11 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglial cells at concentrations ranging from 10 microM to 100 microM.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
authors.forename: Min A
authors.initials: MA
authors.lastname: Lee
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Lee
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
mesh_terms: D029661:Alnus / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D036381:Diarylheptanoids / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C555504:4-hydroxy-2,6-dimethoxyphenyl-6'-O-syringoyl-beta-D-glucopyranoside; C555505:4-hydroxy-2,6-dimethoxyphenyl-6'-O-vanilloyl-beta-D-glucopyranoside; D000893:Anti-Inflammatory Agents; D036381:Diarylheptanoids; D006027:Glycosides; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide