title: Genetic analysis of strains of Japanese Encephalitis Virus isolated from swine in central China.
abstract: In this study, four strains of Japanese Encephalitis Virus (JEV) were isolated from the cerebrospinal fluid of aborted fetuses or stillborn piglets collected randomly from a number of piggeries in central China. The E genes were cloned by RT-PCR and sequenced. Phylogenetic analysis was performed with 48 JEV isolates previously reported in China and other countries, and showed that all four isolates can be classified into the subcluster of genotype III. The results strongly suggest that the genotype III of JEV is the major variant currently circulating in the swinery of central China.
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002 Zhengzhou, People's Republic of China.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Fan
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Man
authors.initials: M
authors.lastname: Teng
authors.forename: Dan
authors.initials: D
authors.lastname: Bu
authors.forename: Fang-Yu
authors.initials: FY
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Chuan-Qing
authors.initials: CQ
authors.lastname: Wang
authors.forename: Gai-Ping
authors.initials: GP
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002555:Cerebrospinal Fluid / Q000821:virology; D002681:China; D016000:Cluster Analysis; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000662:veterinary* / Q000821:virology; D008562:Membrane Glycoproteins / Q000235:genetics; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D008562:Membrane Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C080628:glycoprotein E, Japanese encephalitis virus