The comparison of antioxidative/oxidative profile in colostrum, milk and blood of early post-partum cows during their first and second lactation.
The aim of the study was to compare antioxidative/oxidative profile in blood, colostrum and milk of early post-partum cows during their first and second lactation. A total of 19 healthy, primiparous cows were included in experiment and samples were collected during 2 years from the same animals immediately after parturition, 24, 48 h as well as 6 and 12 days later. All parameters including the activity of glutathione peroxidase (GSH-Px), total antioxidant capacity (TAC), the content of vitamin A, C as well as the contents of products of lipid and protein peroxidation were determined by the use of spectrophotometric methods. Comparing the profile within lactation, TAC and GSH-Px activity in blood showed decreasing trends, while parameters of lipid and protein peroxidation fluctuated. All examined parameters in colostrum and milk except from intermediates of lipid peroxidation exhibited increasing trends. These results which showed dynamic changes of antioxidative/oxidative profile not only in blood but also in colostrum/milk within examined period of time suggested appropriate answer of organism to current challenge. Moreover, not uniform but detectable changes between first and second lactation suggested that two consecutive lactations are not the same. Comparing first and second lactation, TAC and parameters of lipid and protein peroxidation in blood showed increasing tendency in second as first lactation while GSH-Px activity was opposite. The content of antioxidative vitamins and SH groups in colostrum/milk showed increasing tendency in second as first lactation, while TAC and content of end products of lipid peroxidation showed opposite trend, and GSH-Px together with intermediates of lipid peroxidation remained stable. Molecular and biochemical background for it require further elucidation.
Albera
E
E
Department of Animal Biochemistry and Physiology, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland.
Kankofer
M
M
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000737:chemistry / Q000378:metabolism; D002417:Cattle / Q000097:blood*; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000737:chemistry / Q000378:metabolism; D007774:Lactation / Q000502:physiology*; D008892:Milk / Q000737:chemistry*; D010084:Oxidation-Reduction; D049590:Postpartum Period; D017392:Thiobarbituric Acid Reactive Substances; D013997:Time Factors; D014801:Vitamin A / Q000737:chemistry / Q000378:metabolism
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D014801:Vitamin A; D005979:Glutathione Peroxidase; D005978:Glutathione; D001205:Ascorbic Acid