HPLC-based activity profiling for GABA(A) receptor modulators: a new dihydroisocoumarin from Haloxylon scoparium.
A new dihydroisocoumarin was isolated from a dichloromethane extract of Haloxylon scoparium with the aid of a functional assay with Xenopus oocytes transiently expressing GABA(A) receptors of defined subunit composition (alpha(1)beta(2)gamma(2S)). Compound 1 induced a maximum potentiation of the chloride currents by 144.6 +/- 35.3% with an EC(50) of 140.2 +/- 51.2 muM.
Li
Yanfang
Y
Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
Plitzko
Inken
I
Zaugg
Janine
J
Hering
Steffen
S
Hamburger
Matthias
M
D027703:Amaranthaceae / Q000737:chemistry*; D000818:Animals; D018118:Chloride Channels / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D018757:GABA Modulators / Q000494:pharmacology; D049934:Isocoumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D009018:Morocco; D009865:Oocytes / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D014982:Xenopus laevis / Q000378:metabolism
C549468:(S)-4-methoxy-7-phenyl-7,8-dihydro(1,3)dioxolo(4,5-g)isochromen-5-one; D018118:Chloride Channels; D018757:GABA Modulators; D049934:Isocoumarins; D011963:Receptors, GABA-A