Reciprocal chromosome painting between white hawk (Leucopternis albicollis) and chicken reveals extensive fusions and fissions during karyotype evolution of accipitridae (Aves, Falconiformes).
Evolutionary cytogenetics can take confidence from methodological and analytical advances that promise to speed up data acquisition and analysis. Drastic chromosomal reshuffling has been documented in birds of prey by FISH. However, the available probes, derived from chicken, have the limitation of not being capable of determining if breakpoints are similar in different species: possible synapomorphies are based on the number of segments hybridized by each of chicken chromosome probes. Hence, we employed FACS to construct chromosome paint sets of the white hawk (Leucopternis albicollis), a Neotropical species of Accipitridae with 2n = 66. FISH experiments enabled us to assign subchromosomal homologies between chicken and white hawk. In agreement with previous reports, we found the occurrence of fusions involving segments homologous to chicken microchromosomes and macrochromosomes. The use of these probes in other birds of prey can identify important chromosomal synapomorphies and clarify the phylogenetic position of different groups of Accipitridae.
de Oliveira
Edivaldo H Correa
EH
Cambridge Resource Centre for Comparative Genomics, Department of Veterinary Medicine, Cambridge University, Cambridge, UK. ehco@ufpa.br
Tagliarini
Marcella M
MM
Rissino
Jorge Dores
JD
Pieczarka
Julio C
JC
Nagamachi
Cleusa Y
CY
O'Brien
Patricia C M
PC
Ferguson-Smith
Malcolm A
MA
D000818:Animals; D002460:Cell Line; D002645:Chickens / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D020223:Chromosome Painting / Q000379:methods*; D019143:Evolution, Molecular; D046428:Hawks / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008677:Metaphase