title: Effects of a high-protein diet on host resistance to Paracoccidioides brasiliensis in mice.
abstract: We investigated the effects of high protein intake on host resistance to Paracoccidioides brasiliensis. Two-d fasted mice were infected with P. brasiliensis and refed on diets with three different levels (54%, 20%, and 5%) of casein. The mice refed the 54% casein diet showed reduced antifungal activity in the spleen and liver as compared with the mice refed the 5% or the 20% casein diet. After infection, increases in spleen and liver mRNA levels of myeloperoxidase, cathepsin-G, and elastase-2 were more profound in the mice refed the 54% casein diet as compared with the mice refed the 5% or the 20% casein diet. Infected mice refed the 54% casein diet exhibited greater interferon (IFN)-gamma production in the spleen and liver and higher levels of thiobarbituric acid reactive substances (TBARSs) in the liver as compared with those refed the 5% casein diet. These results indicate that high protein intake impairs host resistance to P. brasiliensis.
authors.affiliation: Medical Mycology Research Center, Chiba University, Chiba, Japan. motoko.o@faculty.chiba-u.jp
authors.forename: Motoko
authors.initials: M
authors.lastname: Oarada
authors.forename: Miki
authors.initials: M
authors.lastname: Igarashi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tsuzuki
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kamei
authors.forename: Katsuya
authors.initials: K
authors.lastname: Hirasaka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nikawa
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazawa
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Tohru
authors.initials: T
authors.lastname: Gonoi
mesh_terms: D000818:Animals; D002364:Caseins / Q000276:immunology; D056649:Cathepsin G / Q000032:analysis; D004044:Dietary Proteins / Q000008:administration & dosage*; D005260:Female; D054884:Host-Pathogen Interactions / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010228:Paracoccidioides / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000276:immunology*; D009195:Peroxidase / Q000032:analysis; D012697:Serine Endopeptidases / Q000032:analysis; D013154:Spleen / Q000201:enzymology / Q000276:immunology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D002364:Caseins; D004044:Dietary Proteins; D017392:Thiobarbituric Acid Reactive Substances; D007371:Interferon-gamma; D009195:Peroxidase; D012697:Serine Endopeptidases; D056649:Cathepsin G; C117309:pancreatic elastase II