Molecular characterization of Cryptosporidium spp. from HIV infected patients from an urban area of Brazil.
Cryptosporidium spp. are important cause of enteric disease in humans, but may also infect animals. This study describes the relative frequency of several Cryptosporidium species found in human specimens from HIV infected patients in the São Paulo municipality obtained from January to July 2007. Sequence analysis of the products of nested-PCR based on small subunit rRNA and Cryptosporidium oocyst wall protein coding genes revealed 17 (63.0%) isolates of C. hominis, four (14.8%) C. parvum, five (18.5%) C. felis and one (3.7%) C. canis. These findings suggest that, in urban environments of Brazil, the cat adapted C. felis may play a potential role in the zoonotic transmission of cryptosporidiosis whereas the anthroponotic transmission of cryptosporidiosis caused by C. hominis seems to predominate.
Lucca
Patrícia de
Pd
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, SP, São Paulo, Brazil.
De Gaspari
Elizabeth Natal
EN
Bozzoli
Lígia M
LM
Funada
Mikaela Renata
MR
Silva
Sheila Oliveira de Souza
SO
Iuliano
Wilma
W
Soares
Rodrigo Martins
RM
D000818:Animals; D002415:Cats; D003457:Cryptosporidiosis / Q000469:parasitology* / Q000635:transmission; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004285:Dogs; D005243:Feces / Q000469:parasitology; D005838:Genotype; D015658:HIV Infections / Q000469:parasitology*; D006801:Humans; D016133:Polymerase Chain Reaction; D016053:RNA, Protozoan / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics
D016053:RNA, Protozoan; D012335:RNA, Ribosomal