Kinetic and conformational studies of adenosine deaminase upon interaction with oxazepam and lorazepam.
Oxazepam and lorazepam inhibit the adenosine deaminase (ADA) differently. In the case of lorazepam temperature increment causes an increase in the inhibition potency whereas higher temperature reduces the inhibitory effect of oxazepam; which proposes the overall profounder structural changes in the case of lorazepam relative to those caused by oxazepam.
Moosavi-Movahedi
A A
AA
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. moosavi@ibb.ut.ac.ir
Sepassi Tehrani
H
H
Amanlou
M
M
Soltani Rad
M N
MN
Hakimelahi
G H
GH
Tsai
F-Y
FY
Ataie
G
G
Saboury
A A
AA
Ahmad
F
F
Khalafi-Nezhad
A
A
Poursasan
N
N
Sharifizadeh
A
A
D000241:Adenosine / Q000378:metabolism; D000243:Adenosine Deaminase / Q000737:chemistry* / Q000378:metabolism*; D058892:Adenosine Deaminase Inhibitors; D000818:Animals; D014151:Anti-Anxiety Agents / Q000378:metabolism; D000927:Anticonvulsants / Q000378:metabolism; D002417:Cattle; D002942:Circular Dichroism; D003198:Computer Simulation; D004791:Enzyme Inhibitors / Q000378:metabolism*; D006993:Hypnotics and Sedatives / Q000378:metabolism; D007413:Intestinal Mucosa / Q000201:enzymology; D007700:Kinetics; D008024:Ligands; D008140:Lorazepam / Q000378:metabolism*; D008958:Models, Molecular; D010076:Oxazepam / Q000378:metabolism*; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013696:Temperature
D058892:Adenosine Deaminase Inhibitors; D014151:Anti-Anxiety Agents; D000927:Anticonvulsants; D004791:Enzyme Inhibitors; D006993:Hypnotics and Sedatives; D008024:Ligands; D010076:Oxazepam; D000243:Adenosine Deaminase; D000241:Adenosine; D008140:Lorazepam