Murine model of Hirschsprung-associated enterocolitis. I: phenotypic characterization with development of a histopathologic grading system.
OBJECTIVE
The aim of the study was to characterize enterocolitis in the Ednrb-null (Ednrb-/-) mouse with aganglionosis of the colon and to develop and validate a semiquantitative histopathologic grading system to assess enterocolitis.


METHODS
We isolated colon and ileal specimens of Ednrb-/- and control mice (Ednrb+/+) and performed histochemical staining (H&E) on tissue sections. After establishing inflammation grading criteria, 2 blinded pathologists independently assessed the severity and depth of inflammation of proximal colon segments on 2 separate occasions. Interclass correlations (ICCs) and coefficient of variation (CV) were calculated to determine interrater and intrarater agreement. We then prospectively applied the enterocolitis grading system to Ednrb-/- mice that became clinically ill. A cohort of Ednrb-/- mice were observed until they developed clinical illness, at which time they were euthanized and had multiple organ homogenates cultured for bacteria, and colon and small bowel were histopathologically graded for enterocolitis. Spearman's rank correlations comparing enterocolitis scores with level of bacteremia were performed.


RESULTS
Intra- and interrater ICCs of the histologic scoring system were satisfactory (0.61 and 0.94, respectively), as were intra- and interrater CVs (18% and 9%, respectively). Of the Ednrb-/- mice, 65% developed bacteremia. Those with bacteremia had significantly higher enterocolitis scores than those without bacteremia (P < .01). Ednrb-/- mice that developed bacteremia showed a strong positive correlation between total enterocolitis scores and number of bacterial colony forming units in peritoneal lavage, liver, kidney, and aerobic spleen.


CONCLUSIONS
The Ednrb-/- mouse with aganglionosis develops enterocolitis and has features similar to Hirschsprung-associated enterocolitis in humans. Our grading system is a reliable way to assess enterocolitis. By performing microsurgical pull-through, we can now perform controlled, hypothesis-driven, mechanistic studies to evaluate etiologic factors affecting enterocolitis in the Ednrb-/- mouse.
Cheng
Zhi
Z
Division of Pediatric Surgery and The Pediatric Surgery Laboratory, Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Dhall
Deepti
D
Zhao
Lifu
L
Wang
Hanlin L
HL
Doherty
Terence M
TM
Bresee
Catherine
C
Frykman
Philip K
PK
D000818:Animals; D016470:Bacteremia / Q000150:complications / Q000235:genetics / Q000473:pathology*; D001707:Biopsy, Needle; D004195:Disease Models, Animal; D017615:Enteric Nervous System / Q000473:pathology*; D004760:Enterocolitis / Q000150:complications* / Q000382:microbiology / Q000473:pathology*; D005772:Gastrointestinal Transit / Q000235:genetics; D006627:Hirschsprung Disease / Q000150:complications* / Q000235:genetics / Q000473:pathology*; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011336:Probability; D011897:Random Allocation; D044023:Receptor, Endothelin B / Q000235:genetics; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric
D044023:Receptor, Endothelin B