title: Alveolar stability under different combinations of positive end-expiratory pressure and tidal volume: alveolar microscopy in isolated injured rat lungs.
abstract: BACKGROUND
  High positive end-expiratory pressure (PEEP) and low tidal volume (VT) ventilation is thought to be a protective ventilation strategy. It is hypothesized that the stabilization of collapsible alveoli during expiration contributes to lung protection. However, this hypothesis came from analysis of indirect indices like the analysis of the pressure-volume curve of the lung. The purpose of this study was to investigate isolated healthy and injured rat lungs by means of alveolar microscopy, in which combination of PEEP and VT is beneficial with respect to alveolar stability (I-E%).
  
  
  METHODS
  Alveolar stability was investigated in isolated, non-perfused mechanically ventilated rat lungs. Injured lungs were compared with normal lungs. For both groups three PEEP settings (5, 10, 20 cmH2O) were combined with three VT settings (6, 10, 15 ml/kg) resulting in nine PEEP-VT combinations per group. Analysis was performed by alveolar microscopy.
  
  
  RESULTS
  In normal lungs alveolar stability persisted in all PEEP-VT combinations (I-E% (3.2 +/- 11.0)%). There was no significant difference using different settings (P > 0.01). In contrast, alveoli in injured lungs were extremely instable at PEEP levels of 5 cmH2O (mean I-E% 100%) and 10 cmH2O (mean I-E% (30.7 +/- 16.8)%); only at a PEEP of 20 cmH2O were alveoli stabilized (mean I-E% of (0.2 +/- 9.3)%).
  
  
  CONCLUSIONS
  In isolated healthy lungs alveolar stability is almost unaffected by different settings of PEEP and VT. In isolated injured lungs only a high PEEP level of 20 cmH2O resulted in stabilized alveoli whereas lower PEEP levels are associated with alveolar instability.
authors.affiliation: Department of Breast Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China. franklinsh@hotmail.com
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Claudius A
authors.initials: CA
authors.lastname: Stahl
authors.forename: Knut
authors.initials: K
authors.lastname: Moeller
authors.forename: Matthias
authors.initials: M
authors.lastname: Schneider
authors.forename: Steven
authors.initials: S
authors.lastname: Ganzert
authors.forename: Zhan-qi
authors.initials: ZQ
authors.lastname: Zhao
authors.forename: Xiao-wen
authors.initials: XW
authors.lastname: Tong
authors.forename: Josef
authors.initials: J
authors.lastname: Guttmann
mesh_terms: D000818:Animals; D005260:Female; D008168:Lung / Q000473:pathology*; D055370:Lung Injury / Q000473:pathology*; D008853:Microscopy; D011650:Pulmonary Alveoli / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013990:Tidal Volume / Q000502:physiology*