title: [Simultaneous determination of two anthraquinone-O-glucosides in five processed pieces from Rheum palamatum].
abstract: OBJECTIVE
  To compare the contents of two anthraquinone-O-glucosides in five different processed products from Rheum palamatum.
  
  
  METHODS
  The content of aleo-emodin-8-O-beta-D-glucoside, rhein-8-O-beta-D-glucoside were determined simultaneously by HPLC on a agilent TC-C18 (2) column at 35 degrees C with the mobile phase of acetonitrile-1% glacial acetic acid (20: 80). The detection wavelength was set at 410 nm and the flow rate was 1.0 mL x min(-1).
  
  
  RESULTS
  The calibration curves for two components were linear within the range of 0.017 44-0.348 8 microg (r = 0.999 8, n = 5), 0.084-1.686 microg (r = 0.999 9, n = 5), and the average recoveries were 97.82%, 97.87%, respectively.
  
  
  CONCLUSIONS
  The results showed that there were remarkable variation of the two kinds of glucosides contentsamong the five different processed pieces. The contents of the crude pieces, pieces roasted with millet wine and pieces roasted with vinegar were almost the same, while the prepared pieces and charring pieces had evidently reduced contents. The are certain regularity in the variation.
authors.affiliation: Institute of Chinese Materia Madica, China Academy of Chinese Medicial Sciences, Beijing 100700, China.
authors.forename: Cun
authors.initials: C
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Xiao
authors.forename: Na
authors.initials: N
authors.lastname: Lin
authors.forename: Chunfang
authors.initials: C
authors.lastname: Liu
authors.forename: Guofang
authors.initials: G
authors.lastname: Tian
authors.forename: Dongdong
authors.initials: D
authors.lastname: Chen
mesh_terms: D000880:Anthraquinones / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry*; D012250:Rheum / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D005960:Glucosides