Mineral trioxide aggregate stimulates macrophages and mast cells to release neutrophil chemotactic factors: role of IL-1beta, MIP-2 and LTB(4).
OBJECTIVE
In the present study, the role of macrophages and mast cells in mineral trioxide aggregate (MTA)-induced release of neutrophil chemotactic factor was investigated.


METHODS
MTA suspension (50 mg/mL) was plated over inserts on macrophages or mast cells for 90 minutes. Untreated cells served as controls. Cells were washed and cultured for 90 minutes in RPMI without the stimuli. Macrophages and mast cell supernatants were injected intraperitoneally (0.5 mL/cavity), and neutrophil migration was assessed 6 hours later. In some experiments, cells were incubated for 30 minutes with dexamethasone (DEX, 10 muM/well), BWA4C (BW, 100 muM/well) or U75302 (U75, 10 muM/well). The concentration of Leukotriene B(4) (LTB(4)) in the cell-free supernatant from mast cells and macrophage culture was measured by ELISA.


RESULTS
Supernatants from MTA-stimulated macrophages and mast cells caused neutrophil migration. The release of neutrophil chemotactic factor by macrophages and mast cells was significantly inhibited by DEX, BW, or U75. Macrophages and mast cells expressed mRNA for interleukin-1 (IL-1)beta and macrophage inflammatory protein-2 (MIP-2) and the pretreatment of macrophages and mast cells with DEX, BW, or U75 significantly altered IL-1beta and MIP-2 mRNA expression. LTB(4) was detected in the MTA-stimulated macrophage supernatant but not mast cells.


CONCLUSIONS
MTA-induces the release of neutrophil chemotactic factor substances from macrophages and mast cells with participation of IL-1beta, MIP-2, and LTB(4).
Gomes
Alessandra Cristina
AC
Department of Basic Science, Araçatuba Dentistry School, São Paulo State University, Araçatuba, SP, Brazil.
Gomes-Filho
João Eduardo
JE
Oliveira
Sandra Helena Penha
SH
D017607:Aluminum Compounds / Q000276:immunology / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D017610:Calcium Compounds / Q000276:immunology / Q000494:pharmacology*; D054441:Cell Migration Assays, Leukocyte; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D054426:Chemokine CXCL2 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016207:Cytokines / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004338:Drug Combinations; D053583:Interleukin-1beta / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D007975:Leukotriene B4 / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D010087:Oxides / Q000276:immunology / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000276:immunology / Q000494:pharmacology*; D018709:Statistics, Nonparametric
D017607:Aluminum Compounds; D017610:Calcium Compounds; D054426:Chemokine CXCL2; D017077:Culture Media, Conditioned; D016207:Cytokines; D004338:Drug Combinations; D053583:Interleukin-1beta; D016209:Interleukin-8; D010087:Oxides; D012333:RNA, Messenger; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D007975:Leukotriene B4