Epidemiologic features and virus isolation of enteroviruses in Northern Taiwan during 2000-2008.
The susceptibility of five cell lines, RD, MRC-5, MK-2, Hep-2 and A549, for various serotypes of human enteroviruses was evaluated. RD cells were susceptible to most serotypes of enteroviruses, especially for human enterovirus A. Consequently, a high prevalence of human enterovirus A in Taiwan may reflect the relative importance of RD cells in the clinical virology laboratory. However, susceptibilities of RD cells to coxsackievirus A9 and A24, and echovirus 9 and 11 were exceptionally low. Alternatively, MRC-5 or MK-2 cells can improve the isolation of these four enteroviruses.
Tsao
Kuo-Chien
KC
Research Center for Emerging Viral Infections, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan.
Huang
Chung-Guei
CG
Huang
Ya-Ling
YL
Chen
Feng-Chu
FC
Huang
Peng-Nien
PN
Huang
Yuh-Chering
YC
Lin
Tzou-Yen
TY
Shih
Shin-Ru
SR
Chang
Shih-Cheng
SC
D000818:Animals; D002460:Cell Line; D003384:Coxsackievirus Infections / Q000175:diagnosis* / Q000453:epidemiology* / Q000821:virology; D004770:Enterovirus / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D013624:Taiwan / Q000453:epidemiology; D014776:Virus Cultivation / Q000379:methods*