title: [Fabrication of titanium dioxide nanotube array and effects of its osteoblast proliferation and alkaline phosphatase activity].
abstract: OBJECTIVE
  To investigate preparation parameters of TiO2 nanotube layer with anodization, and to evaluate the osteoblast activity on TiO2 nanotube layer in vitro.
  
  
  METHODS
  Titanium dioxide nanotube layer was grown using anodization method. TiO2 nanotube layers with different structure were obtained by controlling the voltage and time of anodization and rinsing process after anodization. Pure titanium without anodization was used as control. Osteoblasts were cultivated on the anodizated TiO2 nanotube, and the growth of osteoblasts was then evaluated and analyzed.
  
  
  RESULTS
  The voltage, duration of anodization and rinsing process following anodization were key factors to affect morphology of TiO2 nanotube layer. The area of osteoblast cultured on TiO2 nanotube layer was larger than that on pure titanium. Results from methylthiazol tetrazolium (MTT) test showed that the proliferation of osteoblasts after 96 h cultivation on TiO2 nanotube layer (0.62 +/- 0.02) was significantly higher than that on unanodizated titanium (0.55 +/- 0.03, P < 0.05). Three weeks later, the alkaline phosphatase (ALP) activity on TiO2 nanotube layer [(130.8 +/- 5.1) A(405)/mg] was significantly higher than that on unanodizated titanium [(109.6 +/- 4.5) A(405)/mg, P < 0.05].
  
  
  CONCLUSIONS
  The structure of TiO2 nanotube layer was greatly associated with anodization voltage. The TiO2 nanotube layer had a positive effect on osteoblast behavior.
authors.affiliation: Department of Prosthodontics, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
authors.forename: Wei-qiang
authors.initials: WQ
authors.lastname: Yu
authors.forename: Xin-quan
authors.initials: XQ
authors.lastname: Jiang
authors.forename: Yi-lin
authors.initials: YL
authors.lastname: Zhang
authors.forename: Fu-qiang
authors.initials: FQ
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D043942:Nanotubes / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D014025:Titanium / Q000737:chemistry
chemical_list: C009495:titanium dioxide; D014025:Titanium; D000469:Alkaline Phosphatase