Surface-patterned electrode bioreactor for electrical stimulation.
We present a microscale cell culture system with an interdigitated microarray of excimer-laser-ablated indium tin oxide electrodes for electrical stimulation of cultured cells. The system has been characterized in a range of geometeries and stimulation regimes via electrochemical impedance spectroscopy and used to culture primary cardiomyocytes and human adipose derived stem cells. Over 6 days of culture with electrical stimulation (2 ms duration, 1 Hz, 180 microm wide electrodes with 200 microm spacing), both cell types exhibited enhanced proliferation, elongation and alignment, and adipose derived stem cells exhibited higher numbers of Connexin-43-composed gap junctions.
Tandon
Nina
N
Department of Biomedical Engineering, Columbia University, 622 west 168th Street, New York, NY 10032, USA.
Marsano
Anna
A
Maidhof
Robert
R
Numata
Keiji
K
Montouri-Sorrentino
Chrystina
C
Cannizzaro
Christopher
C
Voldman
Joel
J
Vunjak-Novakovic
Gordana
G
D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002478:Cells, Cultured; D004558:Electric Stimulation / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D044085:Microfluidics / Q000295:instrumentation*; D032383:Myocytes, Cardiac / Q000502:physiology*; D051381:Rats; D013499:Surface Properties