Protein interaction matrix of Papaya ringspot virus type P based on a yeast two-hybrid system.
Comprehensive analysis of the interactions between 10 mature proteins of Papaya ringspot virus type P (PRSV-P) was carried out based on a yeast two-hybrid system assay (YTHS). We detected 6 interactions between different viral proteins (VPg-P1, VPg-P3, VPg-CI, VPg-CP, NIaPro-CI, and NIb-P3) and 4 self-interactions (HC-Pro, VPg, NIaPro, and CP). These interactions did not show the same directionality as corresponding interactions detected in other potyviruses and consequently, a protein interaction matrix displayed different patterns. This initial map of the protein interactions of PRSV-P allows further study of various viral proteins in order to develop anew strategy to control PRSV-P infection.
Shen
W T
WT
Key Biotechnology Laboratory for Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology (ITBB), Chinese Academy of Tropical Agriculture Sciences (CATAS), Hainan, P.R. China.
Wang
M Q
MQ
Yan
P
P
Gao
L
L
Zhou
P
P
D029441:Carica / Q000821:virology*; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
D014764:Viral Proteins