title: Suppression of murine preadipocyte differentiation and reduction of visceral fat accumulation by a Petasites japonicus ethanol extract in mice fed a high-fat diet.
abstract: We investigated in this study the anti-obesity effect of an extract of Petasites japonicus (a culinary vegetable from Eastern Asia) on a murine adipocyte cell line (3T3-L1) and on diet-induced obesity-prone mice. An ethanol extract of P. japonicus. (PJET) suppressed 3T3-L1 preadipocyte differentiation; however, a hot water extract of P. japonicus (PJHW) exhibited no effect on cell differentiation. PJET significantly attenuated three adipogenetic transcription factors, peroxisome proliferator-activated receptor gamma2, CCAAT/enhancer-binding protein and sterol regulatory element-binding protein 1C, at the mRNA level and suppressed the gene expression of fatty acid synthetase. An experiment with diet-induced obesity-prone C57BL/6J mice showed that PJET lowered the body weight gain and visceral fat tissue accumulation, and ameliorated the plasma cholesterol concentration. These findings suggest that P. japonicus might be an effective food against obesity.
authors.affiliation: Akita Research Institute of Food and Brewing, Araya, Akita, Japan. takayuki@arif.pref.akita.jp
authors.forename: Takayuki
authors.initials: T
authors.lastname: Watanabe
authors.forename: Keishi
authors.initials: K
authors.lastname: Hata
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hiwatashi
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hori
authors.forename: Nao
authors.initials: N
authors.lastname: Suzuki
authors.forename: Hideaki
authors.initials: H
authors.lastname: Itoh
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D001786:Blood Glucose / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage; D064429:Fatty Acid Synthases / Q000378:metabolism; D050152:Intra-Abdominal Fat / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism; D036901:Petasites / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism
chemical_list: D019440:Anti-Obesity Agents; D001786:Blood Glucose; D022762:CCAAT-Enhancer-Binding Proteins; D004041:Dietary Fats; D047495:PPAR gamma; D010936:Plant Extracts; D051780:Sterol Regulatory Element Binding Protein 1; D002784:Cholesterol; D064429:Fatty Acid Synthases