Selection and identification of B-cell epitope on NS1 protein of dengue virus type 2.
NS1 of dengue virus (DENV) is an important non-structural protein, which plays an important role in DENV replication and dengue infection. In this study, using the phage-displayed peptide library screening method and purified anti-DENV2-NS1 polyclonal antibody immunoglobulin G (IgG) as target, which was generated from the purified recombinant expressed DENV2-NS1 protein immunization on rabbit, seven B-cell epitopes of DENV2-NS1 protein were screened. Considering the results of comprehensive bioinformatic analysis on NS1 B-cell epitopes, possible dominant B-cell epitopes are located in amino acids residues 36-45, 80-89, 103-112, 121-130, 187-196, 295-304, and 315-324 of the NS1, and two epitope-based NS1 protein dodecapeptides corresponding to the predominant epitopes (PA10: (36)PESPSKLASA(45) and AA10: (187)AIKDNRAVHA(196)) were chosen for synthesis. Results of binding assay and competitive-inhibition assays indicated the two peptides were the specific epitopes of DENV2-NS1 protein. These epitopes could be useful in understanding the pathogenesis of DENV and as dengue vaccine constituents in further study.
Jiang
Lan
L
Department of Microbiology, Zhongshan School of Medicine, Sun Yat-Sen University, Zhongshan Road 2, Guangzhou 510080, PR China.
Zhou
Jun-Mei
JM
Yin
Yue
Y
Fang
Dan-Yun
DY
Tang
Yun-Xia
YX
Jiang
Li-Fang
LF
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D019295:Computational Biology; D003716:Dengue Virus / Q000276:immunology*; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D019151:Peptide Library; D011817:Rabbits; D017361:Viral Nonstructural Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D018985:Epitopes, B-Lymphocyte; D007074:Immunoglobulin G; C079340:NS1 protein, Dengue virus type 2; D019151:Peptide Library; D017361:Viral Nonstructural Proteins