Heterologous expression of the oxytetracycline biosynthetic pathway in Myxococcus xanthus.
New natural products for drug discovery may be accessed by heterologous expression of bacterial biosynthetic pathways in metagenomic DNA libraries. However, a "universal" host is needed for this experiment. Herein, we show that Myxococcus xanthus is a potential "universal" host for heterologous expression of polyketide biosynthetic gene clusters.
Stevens
D Cole
DC
Department of Chemistry, Syracuse University, Syracuse, New York 132441, USA.
Henry
Michael R
MR
Murphy
Kimberly A
KA
Boddy
Christopher N
CN
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D053898:Biosynthetic Pathways / Q000235:genetics*; D023061:Gene Order; D005810:Multigene Family; D016941:Myxococcus xanthus / Q000235:genetics*; D010118:Oxytetracycline / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D010118:Oxytetracycline