[Selection of suitable polypropylene tubes for DNA testing using real-time PCR].
Polypropylene microtubes (tubes) are generally used for bio-material tests in addition to PCR tests such as genetically modified organism (GMO) testings. However, the choice of suitable tubes is quite important, because it might influence the results: DNA binding and/or elution of chemical substances sometimes occurs. In this study, we established methods to select tubes with the most suitable characteristics for DNA testing.
Shimizu
Eri
E
Fasmac Co., Ltd., 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan.
Futo
Satoshi
S
Masubuchi
Tomoko
T
Minegishi
Yasutaka
Y
Kasahara
Masaki
M
Akiyama
Hiroshi
H
Teshima
Reiko
R
Hino
Akihiro
A
Mano
Jyunichi
J
Furui
Satoshi
S
Kitta
Kazumi
K
D004247:DNA / Q000032:analysis*; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods; D030841:Food, Genetically Modified; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D011126:Polypropylenes
D011126:Polypropylenes; D004247:DNA