title: [Development and use of a domestic test system for diagnosis of tuberculous infection on the basis of quantitative analysis of interferon-gamma induction in whole blood samples in vitro, by using specific recombinant antigens].
abstract: A test system was developed to detect tuberculous infection by qualitative analysis of interferon-gamma (IFN-gamma) in the plasma samples after 20-24-hour incubation of whole blood samples in the presence of Mycobacterium tuberculosis (MBT) antigens: tuberculin PPD and a mixture of the MBT-specific recombinant antigens ESAT-6 and CFP-10. The analysis used 3 test tubes each containing 1 ml of heparinized venous blood, one of which served as a control; the other two test tubes were employed to measure antigen-induced IFN-gamma production. Whether this test system might be used to determine primary tuberculous infection was studied in 277 children and adolescents. The threshold diagnostic IFN-gamma induction level determined in the test tube containing a mixture of the antigens ESAT-6 and CFP-10 was ascertained. Postvaccine allergy was detectable if there was IFN-gamma induction in the test tube containing tuberculin and if there was no diagnostic IFN-gamma level in that containing the antigens ESAT-6 and CFP-10. The diagnostic sensitivity of detection of primary tuberculous infection was 97.6% with 94.4% specificity, which enabled this condition to be differentiated from postvaccine allergy. The level of antigen-induced IFN-gamma may be lower in relatively disseminated forms of pulmonary tuberculosis.
authors.forename: M A
authors.initials: MA
authors.lastname: Vladimirskiĭ
authors.forename: L I
authors.initials: LI
authors.lastname: Mordovskaia
authors.forename: V A
authors.initials: VA
authors.lastname: Aksenova
authors.forename: L K
authors.initials: LK
authors.lastname: Shipina
authors.forename: E I
authors.initials: EI
authors.lastname: Aksenova
authors.forename: O V
authors.initials: OV
authors.lastname: Sergienko
authors.forename: A M
authors.initials: AM
authors.lastname: Liashuk
authors.forename: T V
authors.initials: TV
authors.lastname: Neretina
authors.forename: A S
authors.initials: AS
authors.lastname: Kariagina
authors.forename: V G
authors.initials: VG
authors.lastname: Lunin
authors.forename: E E
authors.initials: EE
authors.lastname: Efremov
authors.forename: G V
authors.initials: GV
authors.lastname: Ignashenkova
authors.forename: T N
authors.initials: TN
authors.lastname: Vlasik
authors.forename: E V
authors.initials: EV
authors.lastname: Ianushevskaia
authors.forename: N M
authors.initials: NM
authors.lastname: Kashirina
mesh_terms: D000293:Adolescent; D000942:Antigens, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000097:blood*; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000097:blood; D012680:Sensitivity and Specificity; D014373:Tuberculin / Q000097:blood; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000175:diagnosis*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; D014373:Tuberculin; D007371:Interferon-gamma