A practical approach to RT-qPCR-Publishing data that conform to the MIQE guidelines.
Given the highly dynamic nature of mRNA transcription and the potential variables introduced in sample handling and in the downstream processing steps (Garson et al. (2009)), a standardized approach to each step of the RT-qPCR workflow is critical for reliable and reproducible results. The MIQE provides this approach with a checklist that contains 85 parameters to assure quality results that will meet the acceptance criteria of any journal (Bustin et al. (2009)). In this paper we demonstrate how to apply the MIQE guidelines (www.rdml.org/miqe) to establish a solid experimental approach.
Taylor
Sean
S
Bio-Rad Laboratories, Inc., Hercules, CA 94547, USA.
Wakem
Michael
M
Dijkman
Greg
G
Alsarraj
Marwan
M
Nguyen
Marie
M
D017408:Guidelines as Topic; D011643:Publishing / Q000592:standards*; D011786:Quality Control; D012313:RNA / Q000592:standards; D012107:Research Design / Q000592:standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D054928:Validation Studies as Topic
C034477:RNA primers; D012313:RNA