Quorum sensing-controlled Evr regulates a conserved cryptic pigment biosynthetic cluster and a novel phenomycin-like locus in the plant pathogen, Pectobacterium carotovorum.
Pectobacterium carotovorum SCRI193 is a phytopathogenic Gram-negative bacterium. In this study, we have identified a novel cryptic pigment biosynthetic locus in P. carotovorum SCRI193 which we have called the Pectobacterium orange pigment (pop) cluster. The pop cluster is flanked by two tRNA genes and contains genes that encode non-ribosomal peptide synthases and polyketide synthase and produces a negatively charged polar orange pigment. Orange pigment production is activated when an adjacent transcriptional activator sharing sequence similarity with the Erwinia virulence regulator (Evr) is overexpressed. Evr was shown to positively activate its own transcription and that of the pigment biosynthetic genes and an unlinked locus encoding a phenomycin homologue. In addition, the expression of Evr and orange pigment production was shown to be regulated by N-(3-oxohexanoyl)-HSL (OHHL) quorum sensing and have a virulence phenotype in potato. Finally, by comparative genomics and Southern blotting we demonstrate that this pigment biosynthetic cluster is present in multiple P. carotovorum spp., Pectobacterium brasiliensis 1692 and a truncated version of the cluster is present in Pectobacterium atrosepticum. The conserved nature of this cluster in P. carotovorum and P. brasiliensis suggests that the pop cluster has an important function in these broad-host-range soft rotting bacteria, which is no longer required in the narrow-host-range P. atrosepticum SCRI1043.
Williamson
Neil R
NR
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK.
Commander
Paul M B
PM
Salmond
George P C
GP
D001426:Bacterial Proteins / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D023061:Gene Order; D005798:Genes, Bacterial; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D016973:Pectobacterium carotovorum / Q000235:genetics / Q000502:physiology*; D010455:Peptides / Q000378:metabolism*; D010860:Pigments, Biological / Q000096:biosynthesis*; D010935:Plant Diseases / Q000382:microbiology; D053038:Quorum Sensing; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000382:microbiology; D014157:Transcription Factors / Q000378:metabolism*; D014774:Virulence
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D010860:Pigments, Biological; D014157:Transcription Factors; C072155:phenomycin