title: First molecular detection of a viral pathogen in Ugandan honey bees.
abstract: Ugandan honey bees (Apis mellifera L.) produce honey, and are key pollinators within commercial crops and natural ecosystems. Real-time RT-PCR was used to screen immature and adult bees collected from 63 beekeeping sites across Uganda for seven viral pathogens. No samples tested positive for Chronic bee paralysis virus, Sacbrood virus, Deformed wing virus, Acute bee paralysis virus, Apis iridescent virus or Israeli acute paralysis virus. However, Black queen cell virus (BQCV) was found in 35.6% of samples. It occurred in adults and larvae, and was most prevalent in the Western highlands, accounting for over 40% of positive results nationally.
authors.affiliation: National Agricultural Research Organisation, National Livestock Resources Research Institute, Tororo, Uganda.
authors.forename: Robert
authors.initials: R
authors.lastname: Kajobe
authors.forename: Gay
authors.initials: G
authors.lastname: Marris
authors.forename: Giles
authors.initials: G
authors.lastname: Budge
authors.forename: Lynn
authors.initials: L
authors.lastname: Laurenson
authors.forename: Guido
authors.initials: G
authors.lastname: Cordoni
authors.forename: Ben
authors.initials: B
authors.lastname: Jones
authors.forename: Selwyn
authors.initials: S
authors.lastname: Wilkins
authors.forename: Andrew G S
authors.initials: AG
authors.lastname: Cuthbertson
authors.forename: Mike A
authors.initials: MA
authors.lastname: Brown
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D007304:Insect Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014454:Uganda; D014777:Virus Diseases / Q000662:veterinary* / Q000821:virology