Development of an insect cell-free system.
Cell-free protein synthesis systems offer production of native proteins with high speed, even for the proteins that are toxic to cells. Among cell-free systems, the system derived from insect cells has the potential to carry out post-translational modifications that are specific to eukaryotic organisms, as occurs in the rabbit reticulocyte system. In this review, we describe development of this insect cell-free system and its applications.
Ezure
Toru
T
Clinical and Biotechnology Business Unit, Life Science Business Department, Analytical and Measuring Instruments Division, Shimadzu Corporation, 1 Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan. ezure@shimadzu.co.jp
Suzuki
Takashi
T
Shikata
Masamitsu
M
Ito
Masaaki
M
Ando
Eiji
E
Utsumi
Toshihiko
T
Nishimura
Osamu
O
Tsunasawa
Susumu
S
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002474:Cell-Free System / Q000737:chemistry* / Q000378:metabolism*; D007313:Insecta / Q000737:chemistry* / Q000378:metabolism*; D015202:Protein Engineering / Q000639:trends*; D011817:Rabbits; D011994:Recombinant Proteins / Q000138:chemical synthesis* / Q000378:metabolism*
D011994:Recombinant Proteins