Urease inhibitors from Hypericum oblongifolium WALL.
The bioassay-guided fractionation of H. oblongifolium has led to the isolation of potent urease inhibitors 1-3. The structures were elucidated by NMR and mass spectroscopic techniques. Compound 2 showed a potent enzyme inhibition activity (IC(50) 20.96 +/- 0.93), which is comparatively higher than that for the standard thiourea (IC(50) 21.01 +/- 0.51 microM). Compounds 1 and 3 also showed a significant activity, with IC(50) 37.95 +/- 1.93 and 138.43 +/- 1.23 microM, respectively. The sub crude fractions (F1, F2, F3, and F4) were tested in vitro for their urease inhibition activity. Fractions F2 and F4 showed significant activity with IC(50) 140.37 +/- 1.93 and 167.43 +/- 3.03 microM, respectively.
Arfan
Mohammad
M
Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan.
Ali
Mumtaz
M
Ahmad
Habib
H
Anis
Itrat
I
Khan
Ajmal
A
Choudhary
M Iqbal
MI
Shah
Muhammad Raza
MR
D057075:Enzyme Assays; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D020902:Hypericum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D013890:Thiourea / Q000737:chemistry; D014510:Urease / Q000037:antagonists & inhibitors* / Q000737:chemistry
D004791:Enzyme Inhibitors; D010936:Plant Extracts; D014510:Urease; D013890:Thiourea