title: Lactate dehydrogenase and oxidative stress activity in primary open-angle glaucoma aqueous humour.
abstract: Lactate dehydrogenase (LDH) and lactate are some of the hypoxy biochemical parameters. Extracellular activity of this enzyme increases under the condition of oxidative stress, since the cell integrity can be disrupted during the lipid peroxidation process. Subsequently that leads to the increase level of the lactic acid and lactic acid salts. The objective of this investigation is establishing the level of LDH, LDH1 (HBDH) and the lactate concentration in aqueous humour in patients with primary open-angle glaucoma. Biochemical analysis have been made by enzymatic-colometric method (lactate) and UV-kinetic method (LDH and HBDH) in aqueous humour of 30 patients (42 eyes) with primary open-angle glaucoma (POAG) and 30 patients (40 eyes) with cataract (the control group). The increased values of lactate and the activity of LDH and HBDH enzyme in aqueous humour of POAG patients in correlation with the control group are the results not only of oxidative stress but also of hypoxy and the mitochondry oxidative function (p<0,001). The increased activity of the examined biochemical parameters in the aqueous humour of the POAG patients points to the fact that other mechanisms, besides IOP, have a role in glaucoma pathogenesis.
authors.affiliation: Ophthalmology Clinic, Clinical Center, Bulevar dr Zorana Djindjica 48, 18000 Nis, Serbia.
authors.forename: Predrag
authors.initials: P
authors.lastname: Jovanovic
authors.forename: Lepsa
authors.initials: L
authors.lastname: Zoric
authors.forename: Ivan
authors.initials: I
authors.lastname: Stefanovic
authors.forename: Boban
authors.initials: B
authors.lastname: Dzunic
authors.forename: Jasmina
authors.initials: J
authors.lastname: Djordjevic-Jocic
authors.forename: Marija
authors.initials: M
authors.lastname: Radenkovic
authors.forename: Maja
authors.initials: M
authors.lastname: Jovanovic
mesh_terms: D000368:Aged; D001082:Aqueous Humor / Q000201:enzymology*; D016022:Case-Control Studies; D002386:Cataract / Q000378:metabolism; D005260:Female; D005902:Glaucoma, Open-Angle / Q000201:enzymology* / Q000209:etiology / Q000503:physiopathology; D006801:Humans; D007429:Intraocular Pressure; D007527:Isoenzymes / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D012307:Risk Factors
chemical_list: D007527:Isoenzymes; D007773:Lactates; D007770:L-Lactate Dehydrogenase; C409667:lactate dehydrogenase 1