[Simultaneous determination of four components in Gastrodia elata by RP-HPLC].
OBJECTIVE
To develop a reversed-phase HPLC method for simultaneous determination of gastrodin, adenosin, 4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde in Rhizoma Gastrodia.


METHODS
A Kromasil C18 column (4.6 mm x 250 mm, 5 microm) was used with a methanol-water-0.1% acetic acid gradient elution system. The eluates were detected at 270 nm, the flow rate was 1.0 mL x min(-1) and the column temperature was 35 degrees C.


RESULTS
The linear range of gastrodin, adenosin, 4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde were 19.1-383 (r = 0.999 9), 0.620-12.4 (r = 0.999 9), 2.45-49.0 (r = 0.999 9), 0.280-5.63 mg x L(-1) (r = 0.999 6), respectively. The average recoveries (n = 9) of the four components were 96.7% -97.7%, RSD < 1.6%.


CONCLUSIONS
The method is accurate, sensitive and reliable for determination of gastrodin, adenosin, 4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde in Rhizoma Gastrodia.
Yan
Baoqing
B
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. ybqyy123@163.com
Zhang
Huifen
H
Pang
Nannan
N
Chen
Xiaohui
X
Yu
Zhiguo
Z
Bi
Kaishun
K
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D031668:Gastrodia / Q000737:chemistry*
D004365:Drugs, Chinese Herbal