Monoclonal antibody MG7 as a screening tool for gastric cancer.
MG7 is a gastric cancer-specific MAb with high specificity and sensitivity. By using MG7 MAb, we found that MG7Ag was increasingly detected in superficial gastritis, atrophic gastritis, intestinal metaplasia, atypical hyperplasia, and gastric cancer, indicating that MG7Ag could be considered an important early warning molecule of gastric cancer and MG7 MAb could be used as a tool for screening gastric cancer. We have developed a new and sensitive system, immuno-realtime PCR, for detection of MG7Ag in the serum. The use of qIPCR assays enabled the detection of MG7Ag in complex biological samples that were poorly accessible by conventional immunoassays.
Chen
Zheng
Z
State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, Shaanxi Province, China.
Hong
Liu
L
Liu
Lin
L
Peng
Daorong
D
Li
Quanjiang
Q
Jin
Bin
B
Qiao
Taidong
T
Wu
Kaichun
K
Fan
Daiming
D
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005756:Gastritis / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D006965:Hyperplasia / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D007124:Immunoenzyme Techniques; D008679:Metaplasia / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C508753:MG7 antigen, human; C503210:MG7 monoclonal antibody