title: Enzyme catalysis in an aqueous/organic segment flow microreactor: ways to stabilize enzyme activity.
abstract: Multiphase flow microreactors benefit from rapid mixing and high mass transfer rates, yet their application in enzymatic catalysis is limited due to the fast inactivation of enzymes used as biocatalysts. Enzyme inactivation during segment flow is due to the large interfacial area between aqueous and organic phases. The Peclet number of the system points to strong convective forces within the segments, and this results in rapid deactivation of the enzyme depending on segment length and flow rate. Addition of surfactant to the aqueous phase or enzyme immobilization prevents the biocatalyst from direct contact with the interface and thus stabilizes the enzyme activity. Almost 100% enzyme activity can be recovered compared to 45% without any enzyme or medium modification. Drop tensiometry measurements point to a mixed enzyme-surfactant interfacial adsorption, and above a certain concentration, the surfactant forms a protective layer between the interface and the biocatalyst in the aqueous compartments. Theoretical models were used to compare adsorption kinetics of the protein to the interface in the segment flow microreactor and in the drop tensiometry measurements. This study is the basis for the development of segment flow microreactors as a tool to perform productive enzymatic catalysis.
authors.affiliation: Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU- Dortmund, Emil-Figge-Strasse 66, 44227 Dortmund, Germany.
authors.forename: Rohan
authors.initials: R
authors.lastname: Karande
authors.forename: Andreas
authors.initials: A
authors.lastname: Schmid
authors.forename: Katja
authors.initials: K
authors.lastname: Buehler
mesh_terms: D000327:Adsorption; D055162:Biocatalysis; D019149:Bioreactors; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D013500:Surface Tension
chemical_list: D004800:Enzymes, Immobilized