Molecular characterization of a calmodulin involved in the signal transduction chain of gravitaxis in Euglena gracilis.
The unicellular flagellate Euglena gracilis shows a negative gravitactic behavior. This is based on physiological mechanisms which in the past have been indirectly assessed. Meanwhile, it was possible to isolate genes involved in the signal transduction chain of gravitaxis. The DNA sequences of five calmodulins were found in Euglena, one of which was only known in its protein structure (CaM.1); the other four are new. The biosynthesis of the corresponding proteins of CaM.1-CaM.5 was inhibited by means of RNA interference to determine their involvement in the gravitactic signal transduction chain. RNAi of CaM.1 inhibits free swimming of the cells and pronounced cell-form aberrations. The division of cells was also hampered. After recovery from RNAi the cell showed precise negative gravitaxis again. Blockage of CaM.3 to CaM. 5 did not impair gravitaxis. In contrast, the blockage of CaM.2 has only a transient and not pronounced influence on motility and cell form, but leads to a total loss of gravitactic orientation for more than 30 days. This indicates that CaM.2 is an element in the signal transduction chain of gravitaxis in E. gracilis. The results are discussed with regard to the current working model of gravitaxis in E. gracilis.
Daiker
Viktor
V
Department of Biology, Plant Ecophysiology, Friedrich-Alexander University, Erlangen, Germany.
Lebert
Michael
M
Richter
Peter
P
Häder
Donat-Peter
DP
D002147:Calmodulin / Q000235:genetics / Q000378:metabolism*; D005056:Euglena gracilis / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D018466:Gravity Sensing / Q000502:physiology*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
D002147:Calmodulin; D015800:Protozoan Proteins