title: Biosynthesis of silver and gold nanoparticles using Brevibacterium casei.
abstract: The present study demonstrates an unprecedented green process for the production of spherical-shaped Au and Ag nanoparticles synthesized and stabilized using a bacterium, Brevibacterium casei. Aqueous solutions of chloroaurate ions for Au and Ag(+) ions for silver were treated with B. casei biomass for the formation of Au nanoparticles (AuNP) and Ag nanoparticles (AgNP). The nanometallic dispersions were characterized by surface plasmon absorbance measuring at 420 and 540 nm for Ag and Au nanoparticles, respectively. Transmission electron microscopy showed the formation of nanoparticles in the range of 10-50 nm (silver), and 10-50 nm (gold). XRD analysis of the silver and gold nanoparticles confirmed the formation of metallic silver and gold. Further analysis carried out by Fourier Transform Infrared Spectroscopy (FTIR), provides evidence for the presence of proteins as possible biomolecules responsible for the reduction and capping agent which helps in increasing the stability of the synthesized silver and gold nanoparticles. The biological activities of the synthesized particles were confirmed based on their stable anti-coagulant effects. The use of bacterium for nanoparticles synthesis offers the benefits of ecofriendliness and amenability for large-scale production.
authors.affiliation: Department of Biotechnology, Kalasalingam University, Anand Nagar, Krishnankoil 626190, Tamilnadu, India.
authors.forename: Kalimuthu
authors.initials: K
authors.lastname: Kalishwaralal
authors.forename: Venkataraman
authors.initials: V
authors.lastname: Deepak
authors.forename: SureshBabu
authors.initials: S
authors.lastname: Ram Kumar Pandian
authors.forename: Muniasamy
authors.initials: M
authors.lastname: Kottaisamy
authors.forename: Selvaraj
authors.initials: S
authors.lastname: BarathmaniKanth
authors.forename: Bose
authors.initials: B
authors.lastname: Kartikeyan
authors.forename: Sangiliyandi
authors.initials: S
authors.lastname: Gurunathan
mesh_terms: D000328:Adult; D000925:Anticoagulants / Q000737:chemistry; D018533:Biomass; D001798:Blood Proteins / Q000378:metabolism; D001951:Brevibacterium / Q000378:metabolism*; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D049449:Luminescence; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D008875:Middle Aged; D036103:Nanotechnology / Q000379:methods*; D010777:Photochemistry / Q000379:methods; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D000925:Anticoagulants; D001798:Blood Proteins; D012834:Silver; D006046:Gold