title: Human infection with a triple-reassortant swine influenza A(H1N1) virus containing the hemagglutinin and neuraminidase genes of seasonal influenza virus.
abstract: A reassortant influenza A(H1N1) virus of swine origin distinct from the pandemic H1N1 2009 strain was isolated from 3 patients, all of whom worked at the same large hog operation in Saskatchewan, Canada. The genomic composition of the isolates has not been previously reported, to our knowledge, and was the product of a genetic reassortment between seasonal H1N1 and triple-reassortant influenza virus that emerged in North American swine during the late 1990s. The neuraminidase and hemagglutinin genes of A/Saskatchewan/5350/2009, A/Saskatchewan/5351/2009, and A/Saskatchewan/5131/2009 were derived from human H1N1 virus and were closely related to those of A/Brisbane/59/2007.
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bastien
authors.forename: Nick A
authors.initials: NA
authors.lastname: Antonishyn
authors.forename: Ken
authors.initials: K
authors.lastname: Brandt
authors.forename: Christine E
authors.initials: CE
authors.lastname: Wong
authors.forename: Khami
authors.initials: K
authors.lastname: Chokani
authors.forename: Niki
authors.initials: N
authors.lastname: Vegh
authors.forename: Greg B
authors.initials: GB
authors.lastname: Horsman
authors.forename: Shaun
authors.initials: S
authors.lastname: Tyler
authors.forename: Morag R
authors.initials: MR
authors.lastname: Graham
authors.forename: Frank A
authors.initials: FA
authors.lastname: Plummer
authors.forename: Paul N
authors.initials: PN
authors.lastname: Levett
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D000818:Animals; D001483:Base Sequence; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics*; D007251:Influenza, Human / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics*; D009976:Orthomyxoviridae Infections / Q000662:veterinary / Q000821:virology; D010802:Phylogeny; D016865:Reassortant Viruses / Q000235:genetics* / Q000472:pathogenicity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012525:Saskatchewan / Q000453:epidemiology; D013552:Swine / Q000821:virology
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009439:Neuraminidase