Corilagin inhibits the double strand break-triggered NF-kappaB pathway in irradiated microglial cells.
Microglia, the resident immune cells of the central nervous system (CNS), are activated by various stimuli. Resting microglia are the basis of normal neurogenesis, while activated microglia may inhibit neurogenesis through the production of pro-inflammatory mediators and cytokines. Recent research suggests that microglia are activated by irradiation. This may play a role in radiation-induced brain injury (RIBI). DNA double-strand breaks (DSBs), the most deleterious form of DNA damage after ionizing radiation, may rapidly trigger the activation of the NF-kappaB pathway via p53-induced protein leading to the release of pro-inflammatory mediators and cytokines. Thus, a negative regulator of the NF-kappaB pathway that inhibits radiation-induced microglia activation could be used to treat RIBI. Corilagin, a member of the tannin family, inhibits NF-kappaB pathway activation. In the present study, we examined the inhibitory effects of corilagin on radiation-induced microglia activation using a variety of techniques. Our data suggest that corilagin inhibits radiation-induced microglia activation via suppression of the NF-kappaB pathway and the compound is a potential treatment for RIBI.
Dong
Xiao-Rong
XR
Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan, P.R. China.
Luo
Ming
M
Fan
Li
L
Zhang
Tao
T
Liu
Li
L
Dong
Ji-Hua
JH
Wu
Gang
G
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects* / Q000528:radiation effects; D005455:Fluorescent Antibody Technique; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism; D047348:Hydrolyzable Tannins; D020672:I-kappa B Proteins / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D016328:NF-kappa B / Q000378:metabolism*; D011839:Radiation, Ionizing; D015398:Signal Transduction / Q000187:drug effects* / Q000528:radiation effects*
D005960:Glucosides; D006657:Histones; D047348:Hydrolyzable Tannins; D020672:I-kappa B Proteins; D047908:Intracellular Signaling Peptides and Proteins; C514981:NEMO protein, mouse; D016328:NF-kappa B; C475961:gamma-H2AX protein, mouse; C049096:corilagin