title: Design of malaria diagnostic criteria for the Sysmex XE-2100 hematology analyzer.
abstract: Thick film, the standard diagnostic procedure for malaria, is not always ordered promptly. A failsafe diagnostic strategy using an XE-2100 analyzer is proposed, and for this strategy, malaria diagnostic models for the XE-2100 were developed and tested for accuracy. Two hundred eighty-one samples were distributed into Plasmodium vivax, P. falciparum, and acute febrile syndrome groups for model construction. Model validation was performed using 60% of malaria cases and a composite control group of samples from AFS and healthy participants from endemic and non-endemic regions. For P. vivax, two observer-dependent models (accuracy = 95.3-96.9%), one non-observer-dependent model using built-in variables (accuracy = 94.7%), and one non-observer-dependent model using new and built-in variables (accuracy = 96.8%) were developed. For P. falciparum, two non-observer-dependent models (accuracies = 85% and 89%) were developed. These models could be used by health personnel or be integrated as a malaria alarm for the XE-2100 to prompt early malaria microscopic diagnosis.
authors.affiliation: Universidad de Antioquia, Medellín, Colombia. germancz81@gmail.com
authors.forename: Germán
authors.initials: G
authors.lastname: Campuzano-Zuluaga
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Alvarez-Sánchez
authors.forename: Gloria Elcy
authors.initials: GE
authors.lastname: Escobar-Gallo
authors.forename: Luz Marina
authors.initials: LM
authors.lastname: Valencia-Zuluaga
authors.forename: Alexandra Marcela
authors.initials: AM
authors.lastname: Ríos-Orrego
authors.forename: Adriana
authors.initials: A
authors.lastname: Pabón-Vidal
authors.forename: Andrés Felipe
authors.initials: AF
authors.lastname: Miranda-Arboleda
authors.forename: Silvia
authors.initials: S
authors.lastname: Blair-Trujillo
authors.forename: Germán
authors.initials: G
authors.lastname: Campuzano-Maya
mesh_terms: D000328:Adult; D000818:Animals; D005260:Female; D006403:Hematologic Tests / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007958:Leukocyte Count; D016778:Malaria, Falciparum / Q000097:blood* / Q000175:diagnosis*; D016780:Malaria, Vivax / Q000097:blood* / Q000175:diagnosis*; D008297:Male; D008954:Models, Biological; D010963:Plasmodium falciparum; D010966:Plasmodium vivax; D011237:Predictive Value of Tests; D055815:Young Adult