title: [Determination of plasma protein binding rate of bufalin].
abstract: OBJECTIVE
  To study plasma protein binding rate of bufalin.
  
  
  METHODS
  HPLC was employed to determine the concentration of bufalin. Plasma protein binding rate studies were conducted by equilibrium dialysis method. The influence of drug concentration and plasma in different species on plasma protein binding rate were studied.
  
  
  RESULTS
  There was no significant difference in the plasma protein binding rates at low, middle and high bufalin concentrations in dilution medium. The protein binding rate of bufalin in human plasma was higher than in the rat plasma.
  
  
  CONCLUSIONS
  Bufalin has higher protein binding extent with both rat plasma and human plasma.
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Chen
authors.forename: Beilei
authors.initials: B
authors.lastname: Tao
authors.forename: Nianping
authors.initials: N
authors.lastname: Feng
authors.forename: Jihui
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D002018:Bufanolides / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008297:Male; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001798:Blood Proteins; D002018:Bufanolides; C022777:bufalin