[Effect of ginsenosides on level of sex hormone receptors in human liver cell line HL-7702].
OBJECTIVE
To investigate the effect of ginsenoside (GSS) in regulating level of sex hormone receptors in human liver cell line HL-7702.


METHODS
The growth of HL-7702 were detected by MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay for choosing the available concentration of GSS; and the effect of GSS on sex hormone receptors in HL-7702 cells were detected by immuno-histochemistry, Western blot and RT-PCR.


RESULTS
GSS significantly enhance the protein and mRNA expressions of estrogen receptor (ER), progesterone receptor (PR), androgen receptor (AR) in HL7702 cell in a dose-dependent manner, the levels of expressions in the GSS treated group were higher than those in the solvent control group respectively (P < 0.05).


CONCLUSIONS
GSS can up-regulate the protein and mRNA expressions of sex hormone receptors in HL-7702.
Li
Yong
Y
Department of Gastroenterology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai. liyong8256@sohu.com
Zhang
Da-li
DL
Wang
Peng
P
D002460:Cell Line; D036145:Ginsenosides / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D011960:Receptors, Estrogen / Q000378:metabolism*
D036145:Ginsenosides; D011960:Receptors, Estrogen