Expression profile of WNT, FZD and sFRP genes in human hematopoietic cells.
Identifying gene expression differences in the Wnt signaling pathway specific to leukemic cells can be hampered by the lack of verified knowledge on the expression of WNT genes in normal blood cells. In this study we aimed to determine the expression profile of human WNT, FZD and sFRP genes in normal adult bone marrow, T and B cells; along with the hematopoietic cell lines K562, HL60, Jurkat and Namalwa. Bone marrow and peripheral blood from 16 donors were evaluated and our results were compared with the GeneNote database expression arrays. In bone marrow, only WNT3, WNT10A, FZD3, FZD7 and sFRP1 genes are constitutively expressed. Lymphocytes express WNT7A, WNT9B, FZD6 and FZD7 in addition to the genes above, but T cells differ in that they lose sFRP1 expression and gain constitutive expression of WNT16. An established "normal" profile of the Wnt genes in various blood cells will provide a fundamental basis for research investigating hematopoiesis and cellular processes during leukemic transformation.
Sercan
Zeynep
Z
Department of Medical Biology and Genetics, Dokuz Eylul University, Izmir, Turkey.
Pehlivan
Melek
M
Sercan
Hakki Ogun
HO
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000378:metabolism*; D045744:Cell Line, Tumor / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D051157:Frizzled Receptors / Q000096:biosynthesis / Q000235:genetics*; D020869:Gene Expression Profiling; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics*; D019169:Jurkat Cells / Q000378:metabolism; D020014:K562 Cells / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051153:Wnt Proteins / Q000096:biosynthesis / Q000235:genetics*; D055815:Young Adult
D051157:Frizzled Receptors; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D051153:Wnt Proteins