[Absorption of albumin in subeschar tissue fluid in early stage after burn in rabbit and its pharmacokinetics].
OBJECTIVE
To observe the change in albumin concentration in the subeschar tissue fluid of rabbits in early stage after burn, and to analyze its regular pattern.


METHODS
Thirty-four adult male New Zealand rabbits were divided into control group and experiment group according to the random number table, with 17 rabbits in each group. Rabbits in experiment group were subjected to 8% TBSA full-thickness scald on the back and were injected with human serum albumin in subeschar tissue serving as tracing albumin. 1.5 mL blood sample was collected at post scald hour (PSH) 2, 4, 8, 16, 24, 48, 72 respectively. Rabbits in control group were dealt with the above-mentioned procedures except for scald. The concentration of tracing albumin was measured with the enzyme-linked immunosorbent assay kit. The concentration of the serum albumin of rabbits were determined with biochemical analyzer. Pharmacokinetics parameters of tracing albumin were calculated with fitting model of 3P97 practical pharmacokinetics calculating program.


RESULTS
(1) Concentration of tracing albumin of rabbits in experiment group was respectively higher than that in control group (P < 0.01) at each time point, and it peaked at PSH 8 [(421 +/- 10) microg/L]. (2) The concentration of serum albumin of rabbits in experiment group decreased in the beginning and increased later, while no significant change was observed in control group. (3) The distribution phase half-life of tracing albumin of rabbits in experiment group (4.0271 h) was about 1/3 of that of the control group (12.0907 h); while the area under the curve in the experiment group (22 336.38 microg.h.mL(-1)) was about 4 times of that in the control group (5827.77 microg.h.mL(-1)).


CONCLUSIONS
The albumin in the subcutaneous tissue could be absorbed into blood circulation in normal conditions. The resorption occurs earlier and faster and more when obvious inflammation occurs (such as deep burn). Exudation and resorption of albumin co-exist in the early stage after burn.
Wang
Zhen
Z
Department of Burns, Guangzhou First Municipal People's Hospital, Guangzhou Medical College, Guangzhou 510180, China.
Rong
Xin-zhou
XZ
Zhang
Tao
T
Yang
Rong-hua
RH
D000418:Albumins / Q000493:pharmacokinetics* / Q000627:therapeutic use*; D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000628:therapy; D004487:Edema / Q000378:metabolism; D005440:Fluid Therapy; D008297:Male; D011817:Rabbits; D040521:Subcutaneous Tissue / Q000378:metabolism*
D000418:Albumins