title: The pan-DAC inhibitor LBH589 is a multi-functional agent in breast cancer cells: cytotoxic drug and inducer of sodium-iodide symporter (NIS).
abstract: New drugs with anti-tumor activity, also able to modify the expression of selected molecules, are under evaluation in breast cancer which is becoming resistant to conventional treatment, or in metastatic disease. The sodium-iodide symporter (NIS), which mediates iodide uptake into thyroid cells, and is the molecular basis of radioiodine imaging and therapy in thyroid cancer, is also expressed in a large portion of breast tumors. Since NIS expression in breast cancer is not sufficient for a significant iodide uptake, drugs able to induce its expression and correct function are under evaluation. In the present study, we report for the first time that the pan-deacetylase (DAC) inhibitor LBH589 (panobinostat) significantly induced NIS, both as mRNA and as protein, through the increase of NIS promoter activity, with the final consequence of obtaining a significant up-take of iodide in MCF7, T47D, and MDA-MB231 breast cancer cells. Moreover, we observed that LBH589 causes a significant reduction in cell viability of estrogen-sensitive and -insensitive breast cancer cells within nanomolar range. The anti-tumor effect of LBH589 is sustained by apoptosis induction and cell cycle arrest in G(2)/M. In conclusion, our data suggest that LBH589 might be a powerful tool in the management of breast cancer due to its multiple effects and support a potential application of LBH589 in the diagnosis and treatment of this disease.
authors.affiliation: Oncological Endocrinology, AOU San Giovanni Battista, University of Turin, Turin, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Fortunati
authors.forename: M G
authors.initials: MG
authors.lastname: Catalano
authors.forename: F
authors.initials: F
authors.lastname: Marano
authors.forename: V
authors.initials: V
authors.lastname: Mugoni
authors.forename: M
authors.initials: M
authors.lastname: Pugliese
authors.forename: O
authors.initials: O
authors.lastname: Bosco
authors.forename: F
authors.initials: F
authors.lastname: Mainini
authors.forename: G
authors.initials: G
authors.lastname: Boccuzzi
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001692:Biological Transport; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D007211:Indoles; D020128:Inhibitory Concentration 50; D007457:Iodine Radioisotopes / Q000378:metabolism; D000077767:Panobinostat; D012333:RNA, Messenger / Q000378:metabolism; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation; D000099209:Sodium-Iodide Symporters
chemical_list: D000970:Antineoplastic Agents; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D007211:Indoles; D007457:Iodine Radioisotopes; D000077767:Panobinostat; D012333:RNA, Messenger; D027981:Symporters; D000099209:Sodium-Iodide Symporters