Ultrasensitive determination of serum albumin using resonance light scattering based on ZnS-polyacrylic acid nanoparticles.
ZnS-polyacrylic acid (ZnS-PAA) was prepared by an in situ polymerization method using nano-ZnS as core in the presence of acrylic acid (AA), and ZnS-PAA nanoparticles was characterized by ultraviolet spectrometry (UV) and transmission electron microscopy (TEM). Based on the significant increase of the resonance light scattering (RLS) intensity with the interaction between nanoparticles and serum albumin, RLS method was developed for the sensitive determination of serum albumin (BSA and HSA). Under optimum conditions, the change of the intensity (DeltaI) of the RLS spectra at lambda=392nm was linearly proportional to the concentration of BSA and HSA. The linear range was 1-100ngmL(-1) for HSA and 1-120ngmL(-1) for BSA, and the limit of detection (LOD) was 0.4ngmL(-1) for HSA and 0.5ngmL(-1) for BSA. This method proved to be very sensitive, rapid, simple and tolerant of most interfering substances.
Peng
Li
L
Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
Li
Chunya
C
Xue
Hongbo
H
Zhan
Guoqing
G
D000180:Acrylic Resins / Q000737:chemistry*; D002138:Calibration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008027:Light; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000032:analysis* / Q000648:ultrastructure; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013440:Sulfides / Q000737:chemistry*; D013997:Time Factors; D017967:Zinc Compounds / Q000737:chemistry*
D000180:Acrylic Resins; D007202:Indicators and Reagents; D012709:Serum Albumin; D013440:Sulfides; D017967:Zinc Compounds; C006903:carbopol 940; C031238:zinc sulfide