Multielectrode array recordings of the vomeronasal epithelium.
Understanding neural circuits requires methods to record from many neurons simultaneously. For in vitro studies, one currently available technology is planar multielectrode array (MEA) recording. Here we document the use of MEAs to study the mouse vomeronasal organ (VNO), which plays an essential role in the detection of pheromones and social cues via a diverse population of sensory neurons expressing hundreds of types of receptors. Combining MEA recording with a robotic liquid handler to deliver chemical stimuli, the sensory responses of a large and diverse population of neurons can be recorded. The preparation allows us to remove the intact neuroepithelium of the VNO from the mouse and stimulate with a battery of chemicals or potential ligands while monitoring the electrical activity of the neurons for several hours. Therefore, this technique serves as a useful method for assessing ligand activity as well as exploring the properties of receptor neurons. We present the techniques needed to prepare the vomeronasal epithelium, MEA recording, and chemical stimulation.
Arnson
Hannah A
HA
Anatomy and Neurobiology, Washington University School of Medicine, USA.
Fu
Xiaoyan
X
Holy
Timothy E
TE
D000818:Animals; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D009415:Nerve Net / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D009831:Olfactory Mucosa / Q000502:physiology; D019147:Vomeronasal Organ / Q000166:cytology / Q000502:physiology*