Conversion of squid pen by Pseudomonas aeruginosa K187 fermentation for the production of N-acetyl chitooligosaccharides and biofertilizers.
Pseudomonas aeruginosa K187, a protease- and chitinase-producing bacterium, exhibited protease and chitinase activity after three and five days of incubation, respectively. The protease and chitinase were both produced by using 1% squid pen powder (SPP) (w/v) as sole carbon and nitrogen source. After fermentation, the deproteinization rate of the recovered squid pen gradually increased up to 68% on the fourth day. After five days of fermentation, the production of GlcNAc, (GlcNAc)(2), (GlcNAc)(3), (GlcNAc)(4) and (GlcNAc)(5) were 1.18mg/mL, 0.76mg/mL, 1.02mg/mL, 0.93mg/mL and 0.90mg/mL, respectively. The culture supernatant of K187 also exhibited activity of enhancing vegetable growth. For Brassica chinensis Linn treated with the fifth day culture supernatant, the total weight and total length increased up to 529% and 148%, respectively, compared to the control group. With this method, the production of protease, chitinase, N-acetyl chitooligosaccharides and biofertilizers may be useful for biological applications.
Wang
San-Lang
SL
Life Sciences Development Center, Tamkang University, Taipei 25137, Taiwan. sabulo@mail.tku.edu.tw <sabulo@mail.tku.edu.tw>
Hsu
Wan-Han
WH
Liang
Tzu-Wen
TW
D000818:Animals; D001937:Brassica / Q000187:drug effects / Q000254:growth & development; D002688:Chitinases / Q000378:metabolism; D049832:Decapodiformes; D005285:Fermentation; D005308:Fertilizers / Q000032:analysis; D017744:Medical Waste Disposal / Q000379:methods*; D009844:Oligosaccharides / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005308:Fertilizers; D017744:Medical Waste Disposal; D009844:Oligosaccharides; D002688:Chitinases; D010447:Peptide Hydrolases