Development of O-antigen gene cluster-specific PCRs for rapid typing six epidemic serogroups of Leptospira in China.
BACKGROUND
Leptospira is the causative agent of leptospirosis. The O-antigen is the distal part of the lipopolysaccharide, which is a key component of outer membrane of Gram-negative bacteria and confers serological specificity. The epidemiology and clinical characteristics of leptospirosis are relative to the serology based taxonomic unit. Identification of Leptospira strains by serotyping is laborious and has several drawbacks.


RESULTS
In this study, the O-antigen gene clusters of four epidemic Leptospira serogroups (serogroup Canicola, Autumnalis, Grippotyphosa and Hebdomadis) in China were sequenced and all genes were predicted in silico. Adding published sequences of two serogroups, Icterohaemorrhagiae (strain Lai and Fiocruz L1-130) and Sejroe (strain JB197 and L550), we identified six O-antigen-specific genes for six epidemic serogroups in China. PCR assays using these genes were developed and tested on 75 reference strains and 40 clinical isolates.


CONCLUSIONS
The results show that the PCR-based assays can be reliable and alternative means for rapid typing of these six serogroups of Leptospira.
Cai
Cheng-Song
CS
Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Zhu
Yong-Zhang
YZ
Zhong
Yi
Y
Xin
Xiao-Fang
XF
Jiang
Xiu-Gao
XG
Lou
Xiao-Li
XL
He
Ping
P
Qin
Jin-Hong
JH
Zhao
Guo-Ping
GP
Wang
Sheng-Yue
SY
Guo
Xiao-Kui
XK
D000372:Agglutination Tests; D002681:China / Q000453:epidemiology; D003198:Computer Simulation; D004196:Disease Outbreaks; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007919:Leptospira / Q000235:genetics*; D007922:Leptospirosis / Q000453:epidemiology / Q000382:microbiology*; D005810:Multigene Family; D019081:O Antigens / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods*
D019081:O Antigens