title: Coencapsulation of oxygen carriers and glucose oxidase in polyelectrolyte complex capsules for the enhancement of D-gluconic acid and delta-gluconolactone production.
abstract: A novel encapsulated oxidative biocatalyst comprising glucose oxidase (GOD) coencapsulated with oxygen carriers within polyelectrolyte complex capsules was developed for the production of D-gluconic acid and delta-gluconolactone. The capsules containing immobilized GOD were produced by polyelectrolyte complexation with sodium alginate (SA) and cellulose sulfate (CS) as polyanions, poly(methylene-co-guanidine) (PMCG) as the polycation, CaCl(2) as the gelling agent and NaCl as the antigelling agent (GOD-SA-CS/PMCG capsules). Poly(dimethylsiloxane) (PDMS) and an emulsion of n-dodecane (DOD) or perfluorodecaline (PFD) with PDMS were used as the oxygen carriers and MnO(2) was used as a hydrogen peroxide decomposition catalyst. Water-soluble PDMS was found to act as both an oxygen carrier and an emulsifier of water-insoluble DOD and PFD. Stable microcapsules could be produced with concentrations of up to 4% (w/w) of PDMS, 10% (w/w) of DOD and PFD, and 25% (w/w) of MnO(2) in the polyanion solution of SA and CS. Roughly a two-fold increase in the GOD activity from 21.0+/-1.1 to 38.4+/-2.0 U*g(-1) and product space-time yields (STY) from 44.3+/-2.0 to 83.4+/-3.4 g*H*day(-1) could be achieved utilizing coencapsulated oxygen carriers compared to GOD encapsulated in the absence of oxygen carriers. This enhanced production does not significantly depend on the selected oxygen carrier under the conditions used in this study.
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry - Center for Glycomics, Slovak Academy of Sciences, Bratislava, Slovak Republic. Marek.Bucko@savba.sk
authors.forename: Marek
authors.initials: M
authors.lastname: Bucko
authors.forename: Peter
authors.initials: P
authors.lastname: Gemeiner
authors.forename: Alica
authors.initials: A
authors.lastname: Vikartovská
authors.forename: Danica
authors.initials: D
authors.lastname: Mislovicová
authors.forename: Igor
authors.initials: I
authors.lastname: Lacík
authors.forename: Ján
authors.initials: J
authors.lastname: Tkác
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000378:metabolism; D000473:Alkanes / Q000378:metabolism; D055162:Biocatalysis; D002214:Capsules / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000378:metabolism; D004339:Drug Compounding; D005466:Fluorocarbons / Q000378:metabolism; D005942:Gluconates / Q000737:chemistry / Q000378:metabolism*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism; D006147:Guanine / Q000031:analogs & derivatives / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism; D007783:Lactones / Q000378:metabolism*; D017895:Manganese Compounds / Q000378:metabolism; D009757:Nylons / Q000737:chemistry / Q000378:metabolism; D063065:Organophosphonates / Q000737:chemistry; D010087:Oxides / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D012995:Solubility
chemical_list: C487368:9-((1-phosphonomethoxycyclopropyl)methyl)guanine; D000464:Alginates; D000473:Alkanes; D002214:Capsules; D004129:Dimethylpolysiloxanes; D005466:Fluorocarbons; D005942:Gluconates; D006603:Hexuronic Acids; D007783:Lactones; D017895:Manganese Compounds; D009757:Nylons; D063065:Organophosphonates; D010087:Oxides; C085707:poly(dimethylsiloxane)-polyamide copolymer; C007548:n-dodecane; C008805:perfluorodecalin; D006147:Guanine; D020723:Glucuronic Acid; D002482:Cellulose; C020254:cellulose sulfate; D005949:Glucose Oxidase; C030691:gluconic acid; D010100:Oxygen; C016552:manganese dioxide; C010730:beta-glucono-1,5-lactone