A new cytotoxic trichothecene macrolide from the endophyte Myrothecium roridum.
A new cytotoxic roridin-type trichothecene macrolide named roritoxin E(1) was characterized from the solid culture of Myrothecium roridum IFB-E091 (residing originally inside Artemisia annua root), together with the known compounds lumichrome (2), (22 E,24 S)-cerevisterol (3), and (22 E,24 R)-6 beta-methoxy-ergosta-7,22-diene-3 beta,5 alpha-diol (4). The structure of the new macrolide (1) was elucidated by a combination of spectroscopic (UV, IR, MS, and 1D and 2D NMR) analyses. Compound 1 was demonstrated to be inhibitory in vitro against the gastric carcinoma SGC-7901 and hepatocarcinoma SMMC-7721 cell lines, with IC (50) values of 0.26 and 10.54 microg/mL, respectively. 5-Fluorouracil co-assayed as a positive control had an IC (50) value of 6.66 microg/mL against SGC-7901 cells, and it demonstrated only a 9.98 % growth inhibition against SMMC-7721 cells at 10 microg/mL.
Shen
Li
L
Medical College, Yangzhou University, Yangzhou, P. R. China.
Wang
Jian Shou
JS
Shen
Hai Jun
HJ
Song
Yong Chun
YC
Tan
Ren Xiang
RX
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D031172:Artemisia annua; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D002277:Carcinoma / Q000188:drug therapy*; D045744:Cell Line, Tumor; D006801:Humans; D006999:Hypocreales / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D008113:Liver Neoplasms / Q000188:drug therapy*; D018942:Macrolides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D015394:Molecular Structure; D018517:Plant Roots; D013274:Stomach Neoplasms / Q000188:drug therapy*; D014255:Trichothecenes / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D001688:Biological Products; D018942:Macrolides; D014255:Trichothecenes; C555503:roritoxin E