The SYBR Green I malaria drug sensitivity assay: performance in low parasitemia samples.
Validation of the sensitivity of the SYBR Green I in vitro test against an enzyme-linked immunosorbent assay (ELISA)-based drug sensitivity assay. Our results suggest that the SYBR Green I assay is a fast and inexpensive malaria drug screening assay for laboratory use. However, because of its lack of sensitivity in whole blood samples its usefulness for testing clinical samples may be limited.
Vossen
Matthias G
MG
Institute of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria. matthias.vossen@meduniwien.ac.at
Pferschy
Sandra
S
Chiba
Peter
P
Noedl
Harald
H
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D052160:Benzothiazoles; D003959:Diamines; D004351:Drug Resistance; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D009930:Organic Chemicals; D018512:Parasitemia / Q000175:diagnosis*; D021261:Parasitic Sensitivity Tests / Q000379:methods; D010963:Plasmodium falciparum / Q000187:drug effects*; D011804:Quinolines; D012680:Sensitivity and Specificity
D000962:Antimalarials; D052160:Benzothiazoles; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I