Inhibition of sodium-calcium exchange by KB-R7943: Dodecylamine and sphingosine in transfected Chinese hamster ovary cells.
Inhibitors of Na(+)/Ca(2+) exchange (NCX) such as KB-R7943 and SEA0400 are thought to act by promoting an inactive state of the exchanger induced by elevated cytosolic Na(+) concentrations (Na(+)-dependent inactivation). This conclusion is based mainly on experiments in excised patches from frog oocytes expressing NCX and has not been fully tested in intact cells. Here we characterize the inhibitory effects of KB-R7943 and other amphiphilic cations on NCX activity in transfected Chinese hamster ovary (CHO) cells expressing the cardiac isoform of the Na(+)/Ca(2+) exchanger (NCX1.1). Cytosolic Na(+) concentrations were varied using the channel-forming ionophore gramicidin. KB-R7943 (10microM) inhibited NCX activity at high (140mM) but not at low (10mM) cytosolic Na(+) concentrations. Cells expressing NCX mutants that were either more (F223E) or less (K229Q) susceptible than the wild-type to Na(+)-dependent inactivation were either hypersensitive or resistant, respectively, to KB-R7943 inhibition. We recently reported that Na-dependent inactivation in transfected CHO cells could be partially but transiently reversed by increasing the external Ca(2+) concentration (Chernysh et al., 2008). The inhibitory effects of KB-R7943 were likewise partially reversed by elevated Ca(2+). Other amphiphilic cations such as dodecylamine (20microM) and sphingosine (10microM) closely mimicked the effects of KB-R7943 on NCX activity. Our results support the hypothesis that KB-R7943 inhibits NCX activity in intact cells by promoting Na(+)-dependent inactivation and suggest that this is a general mode of inhibition by amphiphilic cations.
Condrescu
Madalina
M
Department of Pharmacology & Physiology, University of Medicine and Dentistry of New Jersey, Newark, 07013, United States.
Reeves
John P
JP
D000588:Amines / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D006096:Gramicidin / Q000494:pharmacology; D020033:Protein Isoforms / Q000037:antagonists & inhibitors / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D019831:Sodium-Calcium Exchanger / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013110:Sphingosine / Q000737:chemistry / Q000494:pharmacology*; D013890:Thiourea / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D014162:Transfection
C101670:2-(2-(4-(4-nitrobenzyloxy)phenyl)ethyl)isothiourea methanesulfonate; D000588:Amines; D000900:Anti-Bacterial Agents; D020033:Protein Isoforms; D019831:Sodium-Calcium Exchanger; C110374:sodium-calcium exchanger 1; D006096:Gramicidin; D012964:Sodium; D013890:Thiourea; D013110:Sphingosine; D002118:Calcium; C003935:dodecylamine