Genetic variation in the indoleamine 2,3-dioxygenase gene in pre-eclampsia.
OBJECTIVE
To investigate the contribution of genomic variations in the indoleamine 2,3-dioxygenase (IDO) gene to the onset of pre-eclampsia.


METHODS
We examined sequence variations in the IDO1 gene using placental genomic DNA from 35 pre-eclamptic patients and 32 normotensive pregnant women.


RESULTS
A case-control study revealed that none of the common variants influences the risk of disease. Sequencing of each IDO1 exon in diseased subjects revealed rare variants. This variation, c.-147_150delGAAA, was located within the 5'-untranslated region of the IDO1 gene, and its homozygote was identified only in pre-eclamptic subjects. However, despite the low levels of IDO expression and enzyme activity in the c.-147_150delGAAA homozygote, reporter assays indicated that this variation does not affect gene expression.


CONCLUSIONS
Our findings indicate that genetic alteration of fetal IDO gene does not appear to be a primary cause of pre-eclampsia.
Nishizawa
Haruki
H
Department of Obstetrics and Gynecology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Kato
Takema
T
Ota
Sayuri
S
Nishiyama
Sachie
S
Pryor-Koishi
Kanako
K
Suzuki
Machiko
M
Tsutsumi
Makiko
M
Inagaki
Hidehito
H
Kurahashi
Hiroki
H
Udagawa
Yasuhiro
Y
D000328:Adult; D000818:Animals; D016022:Case-Control Studies; D005260:Female; D014644:Genetic Variation; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics*; D051379:Mice; D010920:Placenta / Q000378:metabolism; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy
D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase