title: Molecular mapping reveals two independent loci conferring resistance to Albugo candida in the east European germplasm of oilseed mustard Brassica juncea.
abstract: White rust caused by Albugo candida (Pers.) Kuntze is a major disease of the oilseed mustard Brassica juncea. Almost all the released varieties of B. juncea in India are highly susceptible to the disease. This causes major yield losses. Hence, there is an urgent need to identify genes for resistance to white rust and transfer these to the existing commercial varieties through marker-assisted breeding. While the germplasm belonging to the Indian gene pool is highly susceptible to the disease, the east European germplasm of B. juncea is highly resistant. In the present study, we have tagged two independent loci governing resistance to A. candida race 2V in two east European lines, Heera and Donskaja-IV. Two doubled haploid populations were used; the first population was derived from a cross between Varuna (susceptible Indian type) and Heera (partially resistant east European line) and the second from a cross between TM-4 (susceptible Indian type) and Donskaja-IV (fully resistant east European line). In both the resistant lines, a single major locus was identified to confer resistance to white rust. In Heera, the resistance locus AcB1-A4.1 was mapped to linkage group A4, while in Donskaja-IV, the resistant locus AcB1-A5.1 was mapped to linkage group A5. In both the cases, closely linked flanking markers were developed based on synteny between Arabidopsis and B. juncea. These flanking markers will assist introgression of resistance-conferring loci in the susceptible varieties.
authors.affiliation: Centre for Genetic Manipulation of Crop Plants, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.
authors.forename: Priya
authors.initials: P
authors.lastname: Panjabi-Massand
authors.forename: Satish Kumar
authors.initials: SK
authors.lastname: Yadava
authors.forename: Pankaj
authors.initials: P
authors.lastname: Sharma
authors.forename: Arvinder
authors.initials: A
authors.lastname: Kaur
authors.forename: Amarendra
authors.initials: A
authors.lastname: Kumar
authors.forename: N
authors.initials: N
authors.lastname: Arumugam
authors.forename: Yashpal Singh
authors.initials: YS
authors.lastname: Sodhi
authors.forename: Arundhati
authors.initials: A
authors.lastname: Mukhopadhyay
authors.forename: Vibha
authors.initials: V
authors.lastname: Gupta
authors.forename: Akshay K
authors.initials: AK
authors.lastname: Pradhan
authors.forename: Deepak
authors.initials: D
authors.lastname: Pental
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000276:immunology; D002874:Chromosome Mapping; D017343:Genes, Plant; D005819:Genetic Markers; D007113:Immunity, Innate / Q000235:genetics*; D009149:Mustard Plant / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D009868:Oomycetes / Q000276:immunology / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D040641:Quantitative Trait Loci
chemical_list: D005819:Genetic Markers