[Determination of chelerythrine in Chelidonium majus by RP-HPLC].
OBJECTIVE
To develop an HPLC method for determination of the content of chelerythrine in Chelidonium majus.


METHODS
Chelerythrine was extracted from the fine powder of the crade with drug methanol and determined by HPLC. The mobile phase was acetonitrile-1% triethylamine (25:75) (adjusted pH to 3 using phosphoric acid) and the detection wavelength was set at 268 nm.


RESULTS
The linear range of calibration curve was 0.051 6-0.516 0 microg (r = 1.000). The average recovery (n = 6) was 103.0% with RSD of 1.2%. Chelerythrine in the sample solution was stable in 8 h and the ruggedness was perfect among 3 different chromatographic columns.


CONCLUSIONS
The method is accurate, sensitive and reliable.
Sun
Nan
N
Dalian Institute for Drug Control, Dalian 116021, China. snsyp@yahoo.com.cn
Yu
Liming
L
D053119:Benzophenanthridines / Q000032:analysis; D028281:Chelidonium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*
D053119:Benzophenanthridines; D004365:Drugs, Chinese Herbal; C016299:chelerythrine