title: [The expression of p16(INK4a) and Ki-67 in atrophic-hyperplastic and dysdysplastic lesions of the vulva].
abstract: The aim of present study is to investigate the immunohistochemical expression of tumor suppressor gene p16(INK4a) and proliferative marker Ki-67 in dysplastic vulvar lesions and nondysplastic ones.
  
  
  METHODS
  30 female patients (mean age = 49.5 +/- 4.5) - Ca in situ (n = 2), VIN (n = 10), lichen sclerorosus (n = 8), squamous cell hyperplasia (n = 1), mixed vulvar dystrophy (n = 5), papilloma (n = 1), condyllomata accuminata (n = 3) are separated into two groups (first group of non-dysplastic- and second group of dysplastic lesions). The immunohistochemical method with monoclonal antibody for identification of p16(INK4a) and Ki-67 was applied.
  
  
  RESULTS
  P16(INK4a) is expressed in 1 patient (5.56%) in first group and in 8 patients (66.67%) in second group (chi2 = 84.93; p < 0.001). Ki-67 is positive in 4 patients (22.22%) in first group and in 12 of cases (100%) in second group (chi2 = 41.3; p < 0.001).
  
  
  CONCLUSIONS
  There is a significant statistical difference between immunohistochemical expression of p16(INK4a) and Ki-67 in HPV-associated dysplastic and non-dysplastic vulvar lesions.
authors.forename: D
authors.initials: D
authors.lastname: Gincheva
authors.forename: C
authors.initials: C
authors.lastname: Tomov
authors.forename: G
authors.initials: G
authors.lastname: Gorchev
authors.forename: M
authors.initials: M
authors.lastname: Nikolova
mesh_terms: D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis* / Q000276:immunology; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000473:pathology; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis* / Q000276:immunology; D008875:Middle Aged; D018307:Neoplasms, Squamous Cell / Q000473:pathology*; D010212:Papilloma / Q000473:pathology; D014844:Vulva / Q000473:pathology*; D014846:Vulvar Neoplasms / Q000473:pathology*
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D019394:Ki-67 Antigen