[Investigation on Fourier transform infrared spectrum of freshly resected cholangiocarcinoma tissue].
To explore the feasibility of quick intraoperative in situ and noninvasive diagnosis of cholangiocarcinoma by Fourier transform infrared (FTIR) spectroscopy, 26 samples of freshly resected cholangiocarcinoma tissues and 43 samples of benign bile duct tissue were collected during surgery. And all fresh samples were measured by FTIR spectroscopy via probe of attenuated total reflection (ATR) without pretreatment immediately after resection. For each spectrum, 12 bands were identified and assigned between 3 800 and 1 000 cm(-1). The peak position was found, and the intensity and area of the bands were measured; The ratios of the intensity and area were calculated. Standard statistic analysis was performed. The results illustrated that the FTIR spectra of malignant tissue were significantly different from those of benign tissue: 1. The bands related to lipid: The peak of 2 925 cm(-1) shifted to lower wave number significantly (P = 0.033); 2. The bands related to nucleic acid and lipids: the ratios of I1 083/ I1 460 (P = 0.005), S1 083 /S1 460 (P = 0.001) and S1 240/S1 460 (P = 0.025) raised significantly, indicating that the relative quantity of nucleic acid to lipids had evidently increased in malignancy. 3. The bands related to protein: The ratios of I1 550/I1 083 (P = 0.000) and S1 550/S1 083 (P = 0.000) reduced significantly, implying that the relative quantity of protein to nucleic acid in malignancy decreased significantly and the secondary structure of protein changed probably. Our primary result illustrated that the FTIR spectroscopy technique maybe a promising method for in situ and quick intraoperative diagnosis of cholangiocarcinoma and has great value for clinical application.
Yin
Gang
G
Department of General Surgery, Peking University Third Hospital, Beijing 100191, China. snowsalt3663@sina.com
Xu
Zhi
Z
Xu
Yi-Zhuang
YZ
Sun
Qi-Gang
QG
Liu
Ya-Qi
YQ
Zhang
Yuan-Fu
YF
Wu
Jin-Guang
JG
Zhou
Xiao-Si
XS
Ling
Xiao-Feng
XF
D018281:Cholangiocarcinoma / Q000175:diagnosis / Q000473:pathology*; D006801:Humans; D008055:Lipids / Q000032:analysis; D009696:Nucleic Acids / Q000032:analysis; D017433:Protein Structure, Secondary; D011506:Proteins / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared
D008055:Lipids; D009696:Nucleic Acids; D011506:Proteins