Comparison of the plating efficiencies and shelf lives of three different commercial buffered charcoal yeast extract media supplemented with alpha-ketoglutaric acid.
The plating efficiencies and shelf lives of locally made buffered charcoal yeast extract medium supplemented with alpha-ketoglutaric acid (BCYEalpha) were compared to those of media made by BD, Hardy, and Remel. Lung homogenates from guinea pigs infected with Legionella pneumophila were plated monthly onto different medium lots. All media performed equally well and had shelf lives of at least 12 months.
Edelstein
Paul H
PH
University of Pennsylvania School of Medicine and Department of Pathology and Laboratory Medicine, and Clinical Microbiology Laboratory, 4 Gates, Hospital of the University of Pennsylvania, 3400 Spruce St., Philadelphia, PA 19104-4283, USA. phe@mail.med.upenn.edu
Edelstein
Martha A C
MA
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002606:Charcoal; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D006168:Guinea Pigs; D006801:Humans; D007656:Ketoglutaric Acids / Q000378:metabolism; D016952:Legionella pneumophila / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D008168:Lung / Q000382:microbiology
D003470:Culture Media; D007656:Ketoglutaric Acids; D002606:Charcoal