title: [Screening and identification of a fungal strain functioning in hydroxylation of puerarin].
abstract: OBJECTIVE
  Puerarin is a Chinese herbal medicine. We isolated a fungus from soil which could convert puerarin to 3'-hydroxypuerarin.
  
  
  METHODS
  Strains were cultivated on potato dextrose agar (PDA). The biotransformation product was extracted with an organic solvent and detected on high performance liquid chromatography(HPLC).
  
  
  RESULTS
  A total of 186 fungal strains were isolated from soil, and strain NT-01 was determined to transform puerarin into 3'-hydroxypuerarin. The product was identified as 3'-hydroxypuerarin by MS and 1H-NMR and 13C-NMR. The morphology characteristic of strain NT-01 was consistent with Gliocladium deliguescens. Phylogenic analysis on sequences showed that strain NT-01 was clustered together with G. deliguescens and G. viride with high boostrap supporting, so that strain NT-01 was identified as Gliocladium sp.
  
  
  CONCLUSIONS
  Gliocladium strain NT-01 can convert puerarin to 3'-hydroxypuerarin.
authors.affiliation: College of Life Science, Nantong University, Nantong 226007, China. ylp325@tom.com
authors.forename: Liping
authors.initials: L
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhu
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yu
authors.forename: Susu
authors.initials: S
authors.lastname: Tang
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Cui
authors.forename: Hui
authors.initials: H
authors.lastname: Ye
mesh_terms: D001711:Biotransformation; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006900:Hydroxylation; D007529:Isoflavones / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012988:Soil Microbiology
chemical_list: D007529:Isoflavones; C033607:puerarin