title: Portacaval anastomosis-induced hyperammonemia does not lead to oxidative stress.
abstract: Ammonia is neurotoxic and believed to play a major role in the pathogenesis of hepatic encephalopathy (HE). It has been demonstrated, in vitro and in vivo, that acute and high ammonia treatment induces oxidative stress. Reactive oxygen species (ROS) are highly reactive and can lead to oxidization of proteins resulting in protein damage. The present study was aimed to assess oxidative status of proteins in plasma and brain (frontal cortex) of rats with 4-week portacaval anastomosis (PCA). Markers of oxidative stress, 4-hydroxy-2-nonenal (HNE) and carbonylation were evaluated by immunoblotting in plasma and frontal cortex. Western blot analysis did not demonstrate a significant difference in either HNE-linked or carbonyl derivatives on proteins between PCA and sham-operated control rats in both plasma and frontal cortex. The present study suggests PCA-induced hyperammonemia does not lead to systemic or central oxidative stress.
authors.affiliation: Neuroscience Research Unit, Université de Montréal (CRCHUM), 1058 St-Denis Street, Montreal, Quebec, H2X 3J4, Canada.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Yang
authors.forename: Cristina R
authors.initials: CR
authors.lastname: Bosoi
authors.forename: Wenlei
authors.initials: W
authors.lastname: Jiang
authors.forename: Mélanie
authors.initials: M
authors.lastname: Tremblay
authors.forename: Christopher F
authors.initials: CF
authors.lastname: Rose
mesh_terms: D000447:Aldehydes / Q000032:analysis / Q000097:blood; D000641:Ammonia / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism / Q000503:physiopathology; D004195:Disease Models, Animal; D005625:Frontal Lobe / Q000378:metabolism / Q000503:physiopathology; D006501:Hepatic Encephalopathy / Q000378:metabolism* / Q000503:physiopathology*; D022124:Hyperammonemia / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology*; D017114:Liver Failure, Acute / Q000150:complications / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D011167:Portacaval Shunt, Surgical / Q000009:adverse effects; D050050:Protein Carbonylation / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000447:Aldehydes; D015415:Biomarkers; D017382:Reactive Oxygen Species; D000641:Ammonia; C027576:4-hydroxy-2-nonenal