Transfection of siRNAs in multiple myeloma cell lines.
RNA interference (RNAi), a valuable tool to specifically silence gene expression, is becoming an indispensable weapon in the arsenal of functional genomics, target validation and gene-specific therapeutic research. We have shown that Streptolysin-O (SLO) reversible permeabilization is an efficient method to deliver small interfering RNAs (siRNAs) to hard-to-transfect human myeloma cell lines. In addition, we have shown that transfection of siRNAs, using the SLO reversible permeabilization, specifically and efficiently induces gene silencing in myeloma cell lines.
Brito
Jose L R
JL
Section of Haemato-Oncology, Institute of Cancer Research, London, UK.
Brown
Nicola
N
Morgan
Gareth J
GJ
D001426:Bacterial Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D055785:Gene Knockdown Techniques; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D013301:Streptolysins / Q000378:metabolism; D014162:Transfection
D001426:Bacterial Proteins; D034741:RNA, Small Interfering; D013301:Streptolysins; C025964:streptolysin O