Evaluation of a modified microscopic direct diagnosis of dermatophytosis.
Here we present a modified protocol for dematophyte diagnosis, utilizing a simple centrifugation step to significantly decrease false-negative results of the original KOH direct microscopy-based technique. Although culture constitutes the gold-standard diagnosis, the time spent for results is a limit. Fast and low-cost techniques are important for infection screening in underdeveloped countries.
Côbo
Eliângela C
EC
Surgical Pathology Service, Hospital das Clínicas, Universidade Federal do Triângulo Mineiro, 38025-180 Uberaba, Minas Gerais, Brazil.
Silva
Juliano C
JC
Cota
Ulisses A
UA
Machado
Juliana R
JR
Castellano
Lucio R
LR
D003883:Arthrodermataceae / Q000166:cytology / Q000302:isolation & purification*; D002498:Centrifugation; D003881:Dermatomycoses / Q000175:diagnosis*; D005188:False Negative Reactions; D006801:Humans; D008853:Microscopy / Q000379:methods*; D009172:Mycology / Q000379:methods*; D012680:Sensitivity and Specificity