Tumor necrosis factor alpha gene variants do not display allelic imbalance in circulating myeloid cells.
Carriage of the TNF -308 A allele (rs1800629 A) has been associated with increased serum TNF-alpha levels, the development of sepsis syndrome, and fatal outcome, in severely traumatized patients (Menges et al., 2008 [1]). Herein, we analysed the putative allelic imbalance of TNF-alpha release from myeloid cells. Circulating peripheral blood cells from healthy human blood donors (n=104) and monocyte-derived macrophages (n=158) were analysed for their ex vivo capacity of TNF-alpha expression. Our findings indicate that carriage of the TNF -308 A allele is not associated with high TNF-alpha expression in circulating human leucocytes and monocyte-derived macrophages. Other cellular sources, e.g. tissue-resident cells like mast cells and/or tissue specific macrophages might be the cellular source of high TNF-alpha serum levels shortly after trauma.
Wienzek
Sandra
S
Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, Giessen, Germany. Sandra.Wienzek@immunologie.med.uni-giessen.de
Kissel
Karin
K
Breithaupt
Kirstin
K
Lang
Christina
C
Nockher
Angelika
A
Hackstein
Holger
H
Bein
Gregor
G
D000293:Adolescent; D000328:Adult; D022981:Allelic Imbalance; D005260:Female; D005838:Genotype; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D022423:Myeloid Cells / Q000166:cytology / Q000502:physiology*; D009504:Neutrophils / Q000166:cytology / Q000276:immunology; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D011446:Prospective Studies; D017452:Receptors, IgG / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism*; D055815:Young Adult
D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha