title: Enhanced bio-hydrogen production by the combination of dark- and photo-fermentation in batch culture.
abstract: In this study, some key factors, for example, diluted ratio of effluents, the ratio of dark-photo bacteria, light intensity and light/dark cycle influencing hydrogen production by combining Clostridium butyricum and immobilized Rhodopseudomonas faecalis RLD-53 in batch culture, were investigated. Experimental results showed the photo-hydrogen yield decreased when increasing diluted ratio from 1:0.5 to 1:3, and it reached the maximum value of 4368 ml-H(2)/l-effluents at the ratio of 1:0.5. When the ratio of dark-photo bacteria was at 1:2, the hydrogen yield reached highest value of 4.946 mol-H(2)/mol-glucose and cumulative hydrogen volume was 5357 ml-H(2)/l-culture during the combination process. When the light intensity was at 10.25 W/m(2), the hydrogen volume of photo-fermentation and the combination process reached maximum value of 4260 ml-H(2)/l-effluents and 5892 ml-H(2)/l-culture, respectively. During the combination process, maximum total hydrogen yield was 5.374 mol-H(2)/mol-glucose. Meanwhile, hydrogen production under light/dark cycle was evaluated.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, No 73 Huanghe Road, 2nd Campus of HIT Box 2614, Harbin 150090, China.
authors.forename: Bing-Feng
authors.initials: BF
authors.lastname: Liu
authors.forename: Nan-Qi
authors.initials: NQ
authors.lastname: Ren
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Xie
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Wan-Qian
authors.initials: WQ
authors.lastname: Guo
authors.forename: De-Feng
authors.initials: DF
authors.lastname: Xing
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002623:Chemistry Techniques, Analytical; D046972:Clostridium butyricum / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry*; D008027:Light; D010777:Photochemistry / Q000379:methods; D012241:Rhodopseudomonas / Q000378:metabolism; D013997:Time Factors
chemical_list: D006859:Hydrogen; D005947:Glucose