title: Previous stress exposure enhances both anxiety-like behaviour and p35 levels in the basolateral amygdala complex: modulation by midazolam.
abstract: Stress exposure induces long lasting neurobiological changes in selected brain areas, which could be associated with the emergence of negative emotional responses. In the present study, previously restrained animals exhibited excessive anxiety one day later in the elevated plus maze. We explore whether stress exposure affects the expression levels of cyclin-dependent kinase 5 (Cdk5) and of its activator protein p35, in diverse amygdaloid nuclei. Stress exposure enhanced p35 levels selectively in the basolateral amygdala (BLA). This up-regulation might be functionally associated with the occurrence of exaggerated anxiety since such emotional response was selectively reversed by an intra-BLA infusion of olomoucine, a Cdk5 inhibitor, 15 min prior to the restraint session. Moreover, pre-treatment with midazolam, a benzodiazepine ligand, not only prevented the excessive anxiety but also attenuated the p35 increase in the BLA of stressed rats. In conclusion, we suggest a pivotal role of the Cdk5/p35 complex, specifically in BLA in the excessive anxiety induced by a previous stressful experience.
authors.affiliation: Departamento de Farmacología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba. IFEC-CONICET, Argentina.
authors.forename: Elena Anahi
authors.initials: EA
authors.lastname: Bignante
authors.forename: Gabriela
authors.initials: G
authors.lastname: Paglini
authors.forename: Victor Alejandro
authors.initials: VA
authors.lastname: Molina
mesh_terms: D000679:Amygdala / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D001007:Anxiety / Q000523:psychology*; D001522:Behavior, Animal / Q000187:drug effects*; D002452:Cell Count; D051360:Cyclin-Dependent Kinase 5 / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007150:Immunohistochemistry; D053765:Interleukin-12 Subunit p35 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007701:Kinetin / Q000494:pharmacology; D008297:Male; D008874:Midazolam / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000523:psychology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D014151:Anti-Anxiety Agents; D004791:Enzyme Inhibitors; D053765:Interleukin-12 Subunit p35; C090046:olomoucine; D051360:Cyclin-Dependent Kinase 5; D007701:Kinetin; D008874:Midazolam