title: Rapid detection of natriuretic peptides by a microfluidic LabChip analyzer with DNA aptamers: Application of natriuretic peptide detection.
abstract: Rapid detection of brain natriuretic peptide (BNP) concentration can be used for the diagnosis of acute heart failure and for the evaluation of the effectiveness of a clinical therapy. We used the systematic evolution of ligands by exponential enrichment method to develop DNA aptamers for BNP whose sequences were determined by cloning method and consensus sequence analysis. A total of eight conserved sequences was identified. By combining the fluorescent-labeled aptamers with fast protein lab-on-chip analysis, we could achieve quantification of BNP concentrations with high speed, sensitivity, and specificity.
authors.forename: Ming-Cheng
authors.initials: MC
authors.lastname: Lin
authors.forename: Jiraporn
authors.initials: J
authors.lastname: Nawarak
authors.forename: Tai-Yuan
authors.initials: TY
authors.lastname: Chen
authors.forename: Hsien-Yu
authors.initials: HY
authors.lastname: Tsai
authors.forename: Jung-Feng
authors.initials: JF
authors.lastname: Hsieh
authors.forename: Supachok
authors.initials: S
authors.lastname: Sinchaikul
authors.forename: Shui-Tein
authors.initials: ST
authors.lastname: Chen