title: Rapid detection of influenza A pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR.
abstract: The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.
authors.affiliation: Central Virology Laboratory, Public Health Services, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer 52621, Israel. hindiyeh@yahoo.com
authors.forename: Musa
authors.initials: M
authors.lastname: Hindiyeh
authors.forename: Daniela
authors.initials: D
authors.lastname: Ram
authors.forename: Michal
authors.initials: M
authors.lastname: Mandelboim
authors.forename: Tal
authors.initials: T
authors.lastname: Meningher
authors.forename: Shira
authors.initials: S
authors.lastname: Hirsh
authors.forename: Jana
authors.initials: J
authors.lastname: Robinov
authors.forename: Virginia
authors.initials: V
authors.lastname: Levy
authors.forename: Sara
authors.initials: S
authors.lastname: Orzitzer
authors.forename: Roberto
authors.initials: R
authors.lastname: Azar
authors.forename: Zehava
authors.initials: Z
authors.lastname: Grossman
authors.forename: Ella
authors.initials: E
authors.lastname: Mendelson
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D000998:Antiviral Agents / Q000494:pharmacology; D024882:Drug Resistance, Viral; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D007251:Influenza, Human / Q000821:virology*; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D009439:Neuraminidase / Q000235:genetics*; D053139:Oseltamivir / Q000494:pharmacology; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014764:Viral Proteins / Q000235:genetics*; D014773:Virology / Q000379:methods*
chemical_list: D000998:Antiviral Agents; D012367:RNA, Viral; D014764:Viral Proteins; D053139:Oseltamivir; C487630:NA protein, influenza A virus; D009439:Neuraminidase