[Cytoskeleton rearrangements and induced apoptosis in human umbilical venous endothelial cell and WISH cell lines during the invasion of Staphylococcus aureus].
OBJECTIVE
To investigate the changes of cytoskeleton and induced apoptosis in human umbilical venous endothelial cells (HUVEC) and WISH cells during the invasion of Staphylococcus aureus.


METHODS
S. aureus suspension was collected routinely and used to infect HUVEC and WISH cells for 10, 30, 60 and 120 min respectively. The cell-invading ability of S. aureus was observed by microscope and the rearrangement of cytoskeleton of these cells observed under fluorescent microscope. DAPI fluorescent staining and DNA agarose electrophoresis were performed to analyze the apoptosis in HUVEC cells induced by S. aureus.


RESULTS
There was bacterial invasion after staphylococcus aureus was co-incubated with HUVEC and WISH cells for 10 min. The rates of infection were (54.9 +/- 2.4)% and (56.1 +/- 2.4)% at 60 min respectively. The ratios of F-actin rearrangements at 10 min, 30 min, 60 min and 120 min after the invasion of HUVEC and WISH cells with S. aureus were different at (54.7 +/- 2.8)%, (63.0 +/- 2.9)%, (71.0 +/- 2.6)%, (39.5 +/- 2.7)% and (63.3 +/- 2.6)%, (65.0 +/- 2.9)%, (77.0 +/- 2.4)% and (44.0 +/- 1.8)% respectively. The ratios of F-actin rearrangements at 10 min, 30 min and 60 min were higher than those at 120 min (P < 0.05). There was no change of microtubule observed in both cells at the same time. The apoptotic appearance was observed after the invasion of HUVEC cells with s. aureus at 60 min.


CONCLUSIONS
S. aureus may invade the HUVEC and WISH cells through F-actin rearrangement. Apoptosis is induced in HUVEC cells at 60 min.
Liu
Xia
X
Medical School of Jiaxing College, Jiaxing 314001, China.
Miao
Zheng-you
ZY
Xu
Shui-ling
SL
Li
Ying-long
YL
D000199:Actins / Q000378:metabolism*; D000650:Amnion / Q000166:cytology; D017209:Apoptosis; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D013211:Staphylococcus aureus / Q000472:pathogenicity*; D014471:Umbilical Veins / Q000166:cytology
D000199:Actins