title: [Analysis on chemical components of volatile oil and determination of thymoquinone from seed of Nigella glandulifera].
abstract: OBJECTIVE
  To analysis chemical components of volatile oil from the seed of Nigella glandulifera (NG), comparing them with those from the seed of foreign N. sativa (NS) and N. damascene (ND), and to quantify thymoquinone in the volatile oil extracted by hydrodistillation (HD) from the seed of NG.
  
  
  METHODS
  The volatile oil was extracted by supercritical CO2 extraction (SFE-CO2 ) and HD from the seed of NG and its chemical components was analysed by GC-MS, the relative percentage of components were determined by peak aera normalization method and compare with those of the seed of NS and ND. The content of the thymoquinone in the volatile oil was determined by one point external standard method.
  
  
  RESULTS
  In terms of the volatile compounds, p-cymene is the major component of NG and NS, their relative percentage contents are 33.75% and 61.48%, respectively. beta-Elemene is the major component of ND, its relative percentage content is 73.24%. The relative percentage contents of thymoquinone are 3.73% (HD), 3.80% and 0.08% in NG, NS and ND, respectively. Linoleic acid is a major component of volatile oil by SFE-CO2 in NG, but its content of p-cymene is lower. The absolute percentage content of thymoquinone is 1.58% by HD in volatile oil of NG.
  
  
  CONCLUSIONS
  There are comparatively large differences of volatile components in NG, NS and ND.
authors.affiliation: Institute for Drug and Instrument Control of Xinjiang Military Command, Urumqi 830063, China. dongsheng811@sina.com
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Geng
authors.forename: Shufeng
authors.initials: S
authors.lastname: Zhang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Lan
mesh_terms: D016227:Benzoquinones / Q000032:analysis*; D031844:Nigella / Q000737:chemistry*; D009822:Oils, Volatile / Q000032:analysis*; D010938:Plant Oils / Q000032:analysis*; D012639:Seeds / Q000737:chemistry
chemical_list: D016227:Benzoquinones; D009822:Oils, Volatile; D010938:Plant Oils; C003466:thymoquinone