[Determination of caryophyllene in Eupatorium fortune].
OBJECTIVE
To develop a GC method to determine the content of caryophyllene in Eupatorium fortune.


METHODS
The samples were determined on a DB-1701 (0.32 mm x 30 m, 0.25 microm) quartz capillary column. And the sample was extracted with ethanol by the ultrasonic assisted extraction.


RESULTS
The calibration curve of caryophyllene is liner over the range of 0.002-2.0 g x L (-1) (R2 = 1). The recovery was from 96.76% to 104.15%.


CONCLUSIONS
The method is accurate, simple with a good reproducibility. It can be used to control the quality of E. fortune.
Liu
Dongjing
D
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100193, China. liudongjing1101@sina.com
Zhao
Xiaohong
X
Xue
Jian
J
Chen
Shilin
S
Bian
Qingya
Q
D002849:Chromatography, Gas / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D036602:Eupatorium / Q000737:chemistry*; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C024714:caryophyllene