Step-wise refolding of recombinant proteins.
Protein refolding is still on trial-and-error basis. Here we describe step-wise dialysis refolding, in which denaturant concentration is altered in step-wise fashion. This technology controls the folding pathway by adjusting the concentrations of the denaturant and other solvent additives to induce sequential folding or disulfide formation.
Tsumoto
Kouhei
K
Department of Medical Genome Sciences, Graduate School of Engineering, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa 277-8562, Japan. tsumoto@k.u-tokyo.ac.jp
Arakawa
Tsutomu
T
Chen
Linda
L
D002479:Inclusion Bodies / Q000737:chemistry*; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry* / Q000302:isolation & purification*
D011994:Recombinant Proteins