Surface chemistry and polymer film thickness effects on endothelial cell adhesion and proliferation.
Adherence and growth rates of human aortic endothelial cells (HAEC) on plasma polymerized poly(vinylacetic acid) films were measured as functions of the surface density of --COOH groups and plasma deposited film thickness. Pulsed plasma polymerization was employed to produce films containing 3.6 to 9% --COOH groups, expressed as a percent of total carbon content. Endothelial cells exhibited increased cell adherence and proliferation with increasing --COOH surface densities. Additionally, and unexpectedly, cell growth was also dependent on the film thicknesses, which ranged from 25 to 200 nm. The results indicate that optimization of the functional group surface density and film thickness could produce significant enhancements in initial adhesion and subsequent growth of the HAEC cells.
Bhattacharyya
Dhiman
D
Department of Chemistry and Biochemistry, University of Texas at Arlington, PO Box 19065, Arlington, Texas 76019-0065, USA.
Xu
Hao
H
Deshmukh
Rajendra R
RR
Timmons
Richard B
RB
Nguyen
Kytai T
KT
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014841:von Willebrand Factor / Q000378:metabolism
D001672:Biocompatible Materials; D005353:Fibronectins; D011108:Polymers; D011145:Polyvinyls; D014841:von Willebrand Factor; C013215:polyvinyl acetate