title: Use of avian bornavirus isolates to induce proventricular dilatation disease in conures.
abstract: Avian bornavirus (ABV) is a newly discovered member of the family Bornaviridae that has been associated with the development of a lethal neurologic syndrome in birds, termed proventricular dilatation disease (PDD). We successfully isolated and characterized ABV from the brains of 8 birds with confirmed PDD. One isolate was passed 6 times in duck embryo fibroblasts, and the infected cells were then injected intramuscularly into 2 healthy Patagonian conures (Cyanoliseus patagonis). Clinical PDD developed in both birds by 66 days postinfection. PDD was confirmed by necropsy and histopathologic examination. Reverse transcription-PCR showed that the inoculated ABV was in the brains of the 2 infected birds. A control bird that received uninfected tissue culture cells remained healthy until it was euthanized at 77 days. Necropsy and histopathologic examinations showed no abnormalities; PCR did not indicate ABV in its brain tissues.
authors.affiliation: Texas A&M University, College Station, Texas 77843-4467, USA.
authors.forename: Patricia
authors.initials: P
authors.lastname: Gray
authors.forename: Sharman
authors.initials: S
authors.lastname: Hoppes
authors.forename: Paulette
authors.initials: P
authors.lastname: Suchodolski
authors.forename: Negin
authors.initials: N
authors.lastname: Mirhosseini
authors.forename: Susan
authors.initials: S
authors.lastname: Payne
authors.forename: Itamar
authors.initials: I
authors.lastname: Villanueva
authors.forename: H L
authors.initials: HL
authors.lastname: Shivaprasad
authors.forename: Kirsi S
authors.initials: KS
authors.lastname: Honkavuori
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
authors.forename: Thomas
authors.initials: T
authors.lastname: Briese
authors.forename: Sanjay M
authors.initials: SM
authors.lastname: Reddy
authors.forename: Ian
authors.initials: I
authors.lastname: Tizard
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000821:virology*; D028981:Bornaviridae / Q000302:isolation & purification / Q000472:pathogenicity*; D002478:Cells, Cultured; D004108:Dilatation, Pathologic; D004372:Ducks / Q000196:embryology; D005347:Fibroblasts / Q000821:virology; D010311:Parrots / Q000821:virology*; D011531:Proventriculus / Q000473:pathology* / Q000503:physiopathology / Q000821:virology