title: [DNA-EGS1386 in cells induced RNase P inhibits the expression of human cytomegalovirus UL49 gene].
abstract: External Guide Sequences (EGSs) represents a novel nucleic acid based gene interference approach to modulate gene expression. They are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA. DNA-based EGS1386 with a size of 12 nt was chemically synthesized to target the mRNA coding for the UL49 gene of human cytomegalovirus (HCMV). The DNA-based EGS1386 molecule efficiently directed human RNase P to cleave the target mRNA sequence in vitro. A reduction of more than 50% in the levels of UL49 expression was observed in human cells treated with the DNA-based EGS1386 targeted UL49 assayed by fluorescent quantization PCR and Western blotting. This results showed that the DNA-EGS1386 can effectively guide the RNase P cut the target mRNA. Therefore, DNA-EGS can develop into a new gene silencing technology and potential of the anti-viral reagents.
authors.affiliation: National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Cui
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Zeng
authors.forename: Hongjian
authors.initials: H
authors.lastname: Li
authors.forename: Yueqin
authors.initials: Y
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Dan
authors.initials: D
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zou
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Tianhong
authors.initials: T
authors.lastname: Zhou
mesh_terms: D001483:Base Sequence; D003587:Cytomegalovirus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D003586:Cytomegalovirus Infections / Q000201:enzymology / Q000821:virology; D004279:DNA, Viral / Q000235:genetics*; D019020:Directed Molecular Evolution / Q000379:methods; D015967:Gene Expression Regulation, Viral; D006801:Humans; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043262:Ribonuclease P / Q000235:genetics / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism; D058727:RNA, Small Untranslated
chemical_list: D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D015678:Viral Structural Proteins; D043262:Ribonuclease P; D058727:RNA, Small Untranslated