Determination of alternate splicing events using the Affymetrix Exon 1.0 ST arrays.
Alternative splicing plays an important role in regulation of normal cellular function. Alternative splicing of pre-mRNA leads to the diversity of downstream protein products in the cell. The Affymetrix Exon arrays allow for a high throughput evaluation of the differences in spliced mRNA expressed in a biological system. In this study, we describe a method using this technology to study the generation of alternative mRNA transcripts in breast cancer cells that differ in the levels of a particular integrin, alpha3beta1.
Subbaram
Sita
S
Center for Cell Biology and Cancer Research, Albany Medical College, Albany, NY, USA.
Kuentzel
Marcy
M
Frank
David
D
Dipersio
C Michael
CM
Chittur
Sridar V
SV
D017398:Alternative Splicing; D000818:Animals; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002469:Cell Separation; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism; D005091:Exons / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism; D013194:Staining and Labeling
D018076:DNA, Complementary; D012333:RNA, Messenger; D012313:RNA