title: DHA promotes the neuronal differentiation of rat neural stem cells transfected with GPR40 gene.
abstract: G-protein coupled receptor 40 (GPR40), which is expressed ubiquitously in the human brain and pancreas, is a member of the large family of seven-transmembrane receptors and can be activated by polyunsaturated fatty acid (PUFA), in particular docosahexaenoic acid (DHA). Recent studies have shown that the DHA/GPR40 signaling pathway may be closely related with adult neurogenesis in the hippocampus. Here, reconstructing pEGFP-N1 vector-expressing GPR40 gene in cultured rat neural stem cells, we demonstrated that DHA-induced neuronal differentiation, neurite growth and branching of adult rat stem cells is mediated at least in part through GPR40 and it remains effective even at low concentrations of DHA. Furthermore, we also revealed that DHA/GPR40 induced the PLC/IP3 signaling pathway and therefore modulated intracellular Ca(2+) mobilization independent of extracellular Ca(2+) concentration. This may be involved in neuronal differentiation and neurite growth in rat neural stem cells transfected with GPR40 gene. These data provide a new sight in the future utilization of neural stem cells transplantation.
authors.affiliation: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Dexuan
authors.initials: D
authors.lastname: Ma
authors.forename: Minmin
authors.initials: M
authors.lastname: Zhang
authors.forename: Christian P
authors.initials: CP
authors.lastname: Larsen
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Hua
authors.forename: Tetsumori
authors.initials: T
authors.lastname: Yamashima
authors.forename: Ying
authors.initials: Y
authors.lastname: Mao
authors.forename: Liangfu
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002491:Central Nervous System Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D055495:Neurogenesis / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology; D014162:Transfection; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D002491:Central Nervous System Agents; C473035:G-protein-coupled receptor 40, rat; D043562:Receptors, G-Protein-Coupled; D004281:Docosahexaenoic Acids; D015544:Inositol 1,4,5-Trisphosphate; D010738:Type C Phospholipases; D002118:Calcium