Saccharomyces cerevisiae Hsp30 is necessary for homeostasis of a set of thermal stress response functions.
Saccharomyces cerevisiae Hsp30 is a plasma membrane heat shock protein which is induced by various environmental stress conditions. However functional role of Hsp30 during diverse environmental stressors is not presently known. To gain insight into its function during thermal stress, we have constructed and characterized a hsp30 strain during heat stress. BY4741Deltahsp30 cells were found to be more sensitive compared to BY4741 cells when exposed to a lethal heat stress at 50 degrees Celsius. When budding yeast is exposed to either heat shock or weak organic acid, it inhibits Pma1p activity. In this study we measured the levels of Pma1p in mutant and Wt cells both during optimal temperature and heat shock temperature. We observed that BY4741Deltahsp30 cells showed constitutive reduction of Pma1p. To gain further insights into the role of Hsp30 during heat stress, we compared total protein profile by 2D gel electrophoresis followed by identification of differentially expressed spots by LC-MS. We observed that contrary to that expected from thermal stress induced changes in gene expression, the Deltahsp30mutant maintained elevated levels of Pdc1p, Trx1p and Nbp35p and reduced levels of Atp2p and Sod1p during heat shock. In conclusion, Hsp30 is necessary during lethal heat stress, for the maintenance of Pma1p and a set of thermal stress response functions.
Thakur
Suresh
S
Division of R&D Triesta Sciences HCG Tower, #8, P. Kalinga Rao Road, Sampangi Ram Nagar, Bangalore-560 027, India. suresh.triesta@gmail.com
Chakrabarti
Amitabha
A
D015180:Electrophoresis, Gel, Two-Dimensional; D050890:HSP30 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological
D050890:HSP30 Heat-Shock Proteins; C494390:HSP30 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins