[Determination of norisoboldine in Radix Lindera by RP-HPLC].
OBJECTIVE
To develop a RP-HPLC method for quantitative determination of norisoboldine in Radix Linderae and to provide valuable data for quality control of Radix Linderae.


METHODS
The separation was performed on a Phenomenex Gemini C18 column (4.6 mm x 250 mm, 5 microm) at 25 degrees C using a gradient elution of mobile phase A (0.5% formic acid, adjusted pH 2.25 with triethylamine) and mobile phase B (acetonitrile). The detection wavelength was 280 nm.


RESULTS
The calibration curve showed a good linearity (r = 0.999 9) within test ranges of 0.015-1.509 microg. The average recovery was 99.58% with RSD 1.4%.


CONCLUSIONS
The developed method is simple, accurate and reliable with good repeatability. It is suitable for quality evaluation of Radix Linderae.
Chen
Jianzhong
J
Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
Chou
Guixin
G
Yang
Li
L
Wang
Changhong
C
Wang
Zhengtao
Z
D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D027443:Lindera / Q000737:chemistry*
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; C464745:norisoboldine