Cloning of aprE86-1 gene encoding 27 kDa mature fibrinolytic enzyme from Bacillus amyloliquefaciens CH86-1.
A gene, encoding the major secreted fibrinolytic protein of Bacillus amyloliquefaciens CH86-1, was cloned from the genomic DNA. DNA sequencing showed that the gene, aprE86-1, could direct the synthesis of a mature protein of 275 amino acids long after processing. When aprE86-1 was introduced into B. subtilis, 27 kDa mature protein was produced as expected. The fibrinolytic activity of B. subtilis transformant (TF) was higher than that of B. amyloliquefaciens CH86-1, showing the possibility of increasing fibrinolytic activities of Bacillus strains through genetic engineering.
Lee
Ae Ran
AR
Division of Applied Life Science (BK21) Graduate School, Gyeongsang National University Jinju 660-701, Korea.
Kim
Gyoung Min
GM
Kwon
Gun-Hee
GH
Lee
Kang Wook
KW
Park
Jae-Yong
JY
Chun
Jiyeon
J
Cha
Jaeho
J
Song
Young-Sun
YS
Kim
Jeong Hwan
JH
D000595:Amino Acid Sequence; D001407:Bacillus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D005337:Fibrin / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016415:Sequence Alignment
D001426:Bacterial Proteins; D005337:Fibrin