Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring.
Protein transduction domains (PTDs) are short amino acid sequences that promote their own translocation across the cell plasma membrane and have been studied for possible use in drug delivery and gene therapy. However, no direct method to quantify transduction is available. Here, using a new luciferase-tagged human PTD, we show that cellular uptake levels can be determined in a reliable manner. Furthermore, we show that enhanced in vivo tracking by human PTD can be quantified in a mouse model. This is the first report on the direct quantification of PTD transduction in vitro and in vivo, which will be necessary for studying its possible therapeutic application in drug delivery and gene therapy.
Lee
Mi-Sook
MS
Division of Bio-Imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon, Kangwon-Do 200-701, Republic of Korea.
Kwon
Eun-Hye
EH
Choi
Hoon-Sung
HS
Kwon
Seung-Hae
SH
Lee
Chul-Hyun
CH
Shim
In-Sop
IS
Lee
Sang-Kyou
SK
Her
Song
S
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D016503:Drug Delivery Systems; D016903:Drug Monitoring / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D021381:Protein Transport
D021382:Protein Sorting Signals; D008156:Luciferases