title: A highly controllable reconstituted cell-free system--a breakthrough in protein synthesis research.
abstract: The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.
authors.affiliation: The Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, FSB-401, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanamori
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Takuya
authors.initials: T
authors.lastname: Ueda
mesh_terms: D002474:Cell-Free System / Q000737:chemistry* / Q000378:metabolism*; D015202:Protein Engineering / Q000639:trends*; D011994:Recombinant Proteins / Q000138:chemical synthesis* / Q000378:metabolism*; D013678:Technology, Pharmaceutical / Q000639:trends*
chemical_list: D011994:Recombinant Proteins