Chemical characterization of Lycium barbarum polysaccharides and its inhibition against liver oxidative injury of high-fat mice.
In this study, we investigated chemical structure of Lycium barbarum polysaccharides and its modulatory effect on oxidative stress in high-fat mice. The polysaccharides mainly contained xylose and glucose. Little amount of rhamnose, mannose and galactose was observed. The Lycium barbarum polysaccharides had IR bands at 800-1200 cm(-1), 1450-1800 cm(-1), 2500-3000 cm(-1), and 3200-3600 cm(-1), which were distinctive absorptions of polysaccharides. Rats are fed with high-fat diet for 2 months. Results showed that blood and liver antioxidant enzymes activities and GSH level in model mice significantly decreased, and MDA level significantly increased (P<0.01) compared to normal control mice. Administration of Lycium barbarum polysaccharides significantly increased antioxidant enzymes activities and decreased MDA level in mice (P<0.01) compared to model group.
Wu
Hua-Tao
HT
Department of General Surgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province, PR China.
He
Xue-Jun
XJ
Hong
Ying-Kai
YK
Ma
Tao
T
Xu
Yan-Ping
YP
Li
Hui-Hua
HH
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004041:Dietary Fats / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D005978:Glutathione / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared
D000975:Antioxidants; D004041:Dietary Fats; D004365:Drugs, Chinese Herbal; C500223:lycium barbarum polysaccharide; D009569:Nitric Oxide; D008315:Malondialdehyde; D005978:Glutathione