title: Elevated expression of PGR5 and NDH-H in bundle sheath chloroplasts in C4 flaveria species.
abstract: Cyclic electron transport around PSI has been proposed to supply the additional ATP required for C(4) photosynthesis. To investigate the nature of cyclic electron pathways involved in C(4) photosynthesis, we analyzed tissue-specific expression of PGR5 (PROTON GRADIENT REGULATION 5), which is involved in the antimycin A-sensitive pathway, and NDH-H, a subunit of the plastidial NAD(P)H dehydrogenase complex, in four Flaveria species comprising NADP-malic enzyme (ME)-type C(4), C(3)-C(4) intermediate and C(3) species. PGR5 was highly expressed in the C(4) species and enriched in bundle sheath chloroplasts together with NDH-H, suggesting that electron transport of both PGR5-dependent and NDH-dependent cyclic pathways is promoted to drive C(4) photosynthesis.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, 630-0192 Japan. munekage@bs.naist.jp
authors.forename: Yuri Nakajima
authors.initials: YN
authors.lastname: Munekage
authors.forename: Françoise
authors.initials: F
authors.lastname: Eymery
authors.forename: Dominique
authors.initials: D
authors.lastname: Rumeau
authors.forename: Stephan
authors.initials: S
authors.lastname: Cuiné
authors.forename: Masateru
authors.initials: M
authors.lastname: Oguri
authors.forename: Naoya
authors.initials: N
authors.lastname: Nakamura
authors.forename: Akiho
authors.initials: A
authors.lastname: Yokota
authors.forename: Bernard
authors.initials: B
authors.lastname: Genty
authors.forename: Gilles
authors.initials: G
authors.lastname: Peltier
mesh_terms: D002736:Chloroplasts / Q000378:metabolism*; D004579:Electron Transport / Q000235:genetics / Q000502:physiology; D037142:Flaveria / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D015151:Immunoblotting; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins