Vesicular glutamate transporter 2-immunoreactive synapses onto phrenic motoneurons in the neonatal rat.
The synaptic organization between vesicular glutamate transporter 2 (VGLUT2)-immunoreactive (ir) axon terminals and phrenic motoneurons in the neonatal rat was examined using a combined retrograde tracing and immunohistochemistry for VGLUT2. The phrenic nucleus (PhN) contained large numbers of VGLUT2-ir axon terminals, some of which made axosomatic and axodendritic synapses with PhN motoneurons. These terminals were of asymmetrical type and contained spherical clear synaptic vesicles. The results suggest that in the neonatal rat glutamatergic synapses onto PhN motoneurons exist and mediate excitatory transmission to drive PhN motoneurons.
Yokota
Shigefumi
S
Department of Anatomy a Morphological Neuroscience, Shimane University School of Medicine, Izumo, Japan. yokotas@med.shimane-u.ac.jp
Shinozaki
Yoshio
Y
Oku
Yoshitaka
Y
Okada
Yasumasa
Y
Yasui
Yukihiko
Y
D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000187:drug effects / Q000378:metabolism; D002772:Cholera Toxin / Q000633:toxicity; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009046:Motor Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010791:Phrenic Nerve / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D050599:Vesicular Glutamate Transport Protein 2 / Q000378:metabolism*
D050599:Vesicular Glutamate Transport Protein 2; D002772:Cholera Toxin