[Interaction of the photosensitizer 13,15-N-(3'-hydroxypropyl)cycloimide of chlorin p(6) with normal and cancerous blood cells].
The interaction of 13,15-N-(3'-hydroxypropyl)cycloimide of chlorin p(6) (CIC) with normal blood cells and human K562 and HL60 myeloid leukemia cells was studied. CIC was found to be bound by the erythrocyte membrane but did not penetrate into the cytoplasm. It is characterized by a diffuse distribution in the cytoplasm of normal leukocytes, whereas its diffuse distribution in K562 and HL60 cells is accompanied by perinuclear accumulation and binding to the plasma membrane. The average cytoplasmic concentration corresponding to the CIC accumulation in leukemic cells at saturation is 2.2 to 2.6 times higher than that in normal leukocytes. CIC is more intensely accumulated in granulocytes than in lymphocytes. The kinetics of the cellular uptake and efflux was characterized. The normal leukocytes and erythrocytes were found to be 1.5 times and 3 to 4 times less sensitive, respectively, to the photodynamic action of CIC than the K562 and HL60 cells.
Ignatova
A A
AA
Maslova
A S
AS
Kirpichnikov
M P
MP
Feofanov
A V
AV
D002462:Cell Membrane / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D007700:Kinetics; D007962:Leukocytes / Q000378:metabolism*; D017319:Photosensitizing Agents / Q000493:pharmacokinetics* / Q000494:pharmacology; D011166:Porphyrins / Q000493:pharmacokinetics* / Q000494:pharmacology
C461439:13,15-N-(3'-hydroxypropyl)cycloimide chlorin p6; D017319:Photosensitizing Agents; D011166:Porphyrins