title: A new lipid transfer protein homolog identified as an IgE-binding antigen from Japanese cedar pollen.
abstract: Japanese cedar (Cryptomeria japonica) pollen is a major cause of seasonal rhinitis and conjunctivitis in Japan, and an understanding of its full allergen repertoire is prerequisite for the development of future molecular diagnostics and immunotherapeutic strategies. Here we report the identification of a new C. japonica pollen IgE-binding antigen (CJP-8) homologous to lipid transfer proteins (LTPs), a class of plant cross-reactive allergens found in foods, latex, and pollen grains. The cjp-8 cDNA encodes a 165-amino acid polypeptide possessing the conserved eight cysteines characteristic of plant LTP family members. Escherichia coli-expressed recombinant CJP-8 (r-CJP-8) reacted with IgE antibody from Japanese cedar pollinosis patients at a 37.5% frequency (6/16).
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan.
authors.forename: Ahmed Ragaa Nour
authors.initials: AR
authors.lastname: Ibrahim
authors.forename: Seiji
authors.initials: S
authors.lastname: Kawamoto
authors.forename: Minori
authors.initials: M
authors.lastname: Nishimura
authors.forename: Syunka
authors.initials: S
authors.lastname: Pak
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Aki
authors.forename: Araceli
authors.initials: A
authors.lastname: Diaz-Perales
authors.forename: Gabriel
authors.initials: G
authors.lastname: Salcedo
authors.forename: Juan A
authors.initials: JA
authors.lastname: Asturias
authors.forename: Takaharu
authors.initials: T
authors.lastname: Hayashi
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ono
mesh_terms: D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000235:genetics / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology*; D003001:Cloning, Molecular; D032485:Cryptomeria / Q000235:genetics / Q000276:immunology*; D003545:Cysteine / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D017386:Sequence Homology, Amino Acid
chemical_list: D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; C030363:lipid transfer protein; D007073:Immunoglobulin E; D003545:Cysteine