Effect of experimental diabetes and STZ on male fertility capacity. Study in rats.
To assess the effect of experimental Type 1 diabetes on male fertility, male Sprague Dawley rats were injected with either streptozotocine (STZ) to induce diabetes or with citrate buffer as controls. Diabetic animals and 2 control groups (STZ-resistant and buffer-injected rats) were sacrificed at 2 different times after injection: 6 weeks (6W) and 20 weeks (20W). We analyzed serum testosterone (sTT), epididymal sperm parameters, and weight of testicles and epididymides, and carried out a histological evaluation of testicular tissue. Diabetic animals presented a significant increase in teratozoospermia (20W, P < .01) and a decrease in sTT (P < .01), tubular diameter (6W, P < .05), and testicular (6W, P < .01) and epididymal (P < .01) weight. STZ-resistant animals showed significantly decreased sTT (6W, P < .01), epididymal weight (6W, P < .05), and sperm count (6W, P < .01) compared with buffer-injected controls. Experimental STZ diabetes increases teratozoospermia and decreases sTT, testicular weight (reverting at medium-term), and epididymal weight.
Navarro-Casado
L
L
Complejo Hospitalario Universitario de Albacete, Hermanos Falco 37, Albacete, Spain. lnavarro@sescam.jccm.es
Juncos-Tobarra
M A
MA
Cháfer-Rudilla
M
M
de Onzoño
L Íñiguez
LÍ
Blázquez-Cabrera
J A
JA
Miralles-García
J M
JM
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000150:complications* / Q000473:pathology; D004822:Epididymis / Q000473:pathology / Q000503:physiopathology; D007248:Infertility, Male / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D055101:Semen Analysis; D013737:Testis / Q000473:pathology / Q000503:physiopathology; D013739:Testosterone / Q000097:blood
D013739:Testosterone