The assay of pterostilbene in spiked matrices by liquid chromatography tandem mass spectrometry and isotope dilution method.
Pterostilbene (trans-3,5-dimethoxy-4-hydroxystilbene) is an active component found in several plant species, exhibiting important pharmacological properties. A new and reliable method of assaying this phyto compound in various matrices is presented; the assay is based on (1) the selectivity of liquid chromatography (LC) hyphenated with electrospray ionisation (ESI), (2) the specificity of a two-step mass spectrometric analysis (MS/MS) and (3) the accuracy of the isotope dilution method. The labelled analogue may be conveniently synthesised in a few steps. The sensitivity of the method is confirmed by the very low limit of detection (LOD) and limit of quantitation (LOQ) values achieved in the assay of pterostilbene in two distinct fortified matrices, and is further supported by the observed accuracy values.
Mazzotti
Fabio
F
Dipartimento di Chimica, Università della Calabria, cubo 12/C, I-87036 Arcavacata di Rende (CS), Italy.
Di Donna
Leonardo
L
Benabdelkamel
Hicham
H
Gabriele
Bartolo
B
Napoli
Anna
A
Sindona
Giovanni
G
D029798:Blueberry Plants / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013267:Stilbenes / Q000032:analysis* / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000379:methods*
D013267:Stilbenes; C107773:pterostilbene