[Characterization of follistatin-related protein from the hard tick Haemaphysalis longicornis].
We designed the primers based on the sequence of the follistatin-related protein from Haemaphysalis longicornis Okayama strain accessed in GenBank. We cloned a gene encoding follistatin-related protein by RT-PCR, and the length cDNA is 814 bp, encoding a deduced protein of 289 amino acids. The alignment with the sequence of follistatin-related protein from the H. longicornis Okayama strain showed that the percent of nucleotide sequence and amino acid sequence is 97.8% and 99%, respectively. The expected size of GST-fused recombinant protein was 57 kD. We purified the recombinant protein through MagneGST protein purification system. Western blotting revealed that stronger reaction happened with the antiserum against eggs, but not clear with antisera against other developmental stages.
Tian
Zhancheng
Z
Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Liu
Guangyuan
G
Yin
Hong
H
Luo
Jianxun
J
Xie
Junren
J
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D038702:Follistatin-Related Proteins / Q000235:genetics* / Q000276:immunology*; D026863:Ixodidae / Q000737:chemistry*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D016415:Sequence Alignment
D038702:Follistatin-Related Proteins; D011994:Recombinant Proteins