Nanomagnetic competition assay for low-abundance protein biomarker quantification in unprocessed human sera.
A novel giant magnetoresistive sensor and uniform high-magnetic-moment FeCo nanoparticles (12.8 nm)-based detecting platform with minimized detecting distance was developed for rapid biomolecule quantification from body fluids. Such a system demonstrates specific, accurate, and quick detection and quantification of interleukin-6, a low-abundance protein and a potential cancer biomarker, directly in 4 muL of unprocessed human sera. This platform is expected to facilitate the identification and validation of disease biomarkers. It may eventually lead to a low-cost personal medical device for chronic disease early detection, diagnosis, and prognosis.
Li
Yuanpeng
Y
Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Srinivasan
Balasubramanian
B
Jing
Ying
Y
Yao
Xiaofeng
X
Hugger
Marie A
MA
Wang
Jian-Ping
JP
Xing
Chengguo
C
D015415:Biomarkers / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D003035:Cobalt / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015850:Interleukin-6 / Q000097:blood*; D007501:Iron / Q000737:chemistry; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D013997:Time Factors
D015415:Biomarkers; D014408:Biomarkers, Tumor; D015850:Interleukin-6; D003035:Cobalt; D007501:Iron