Evaluation of synthetic cell-penetrating peptides, Pro-rich peptide and octaargine derivatives, as adenovirus vector carrier.
Two cell-penetrating peptides, a Pro-rich peptide derivative, acetyl-(Val-Arg-Leu-Pro-Pro-Pro)(3)-Gly-Cys amide, and an octaarginine derivative, acetyl-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Cys amide, were prepared by the solid phase method. Each peptide was coupled to the heterobifunctional cross-linking reagent, 6-maleimidohexanoic acid N-hydroxysuccinimide ester, and then conjugated to the Adenovirus vector containing luciferase gene. Peptide-modified Ad, as compared with wild-type Ad, exhibited excellent luciferase activity in B16BL6 cells.
Kida
Shinya
S
Life Science Center, Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Minatojima, Chuo-ku, Kobe 650-8586, Japan.
Eto
Yusuke
Y
Yoshioka
Yasuo
Y
Nakagawa
Shinsaku
S
Kawasaki
Koichi
K
Maeda
Mitsuko
M
D000256:Adenoviridae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000577:Amides / Q000493:pharmacokinetics; D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D003432:Cross-Linking Reagents; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000008:administration & dosage*; D006801:Humans; D051379:Mice; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D011392:Proline / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D011991:Receptors, Virus / Q000378:metabolism; D014161:Transduction, Genetic
D000577:Amides; C568771:CLMP protein, human; C568772:CLMP protein, mouse; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D003432:Cross-Linking Reagents; D009842:Oligopeptides; D010455:Peptides; D011991:Receptors, Virus; C448619:octaarginine; D011392:Proline