Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins.
BACKGROUND
The human immunodeficiency virus type 1 (HIV-1) pandemic has continued unabated for nearly 30 years. To better understand the influence of virus on host cells, we performed the differential proteome research of peripheral blood mononuclear cells (PBMCs) from HIV-positive patients and healthy controls.


RESULTS
26 protein spots with more than 1.5-fold difference were detected in two dimensional electrophoresis (2DE) gels. 12 unique up-regulated and one down-regulated proteins were identified in HIV-positive patients compared with healthy donors. The mRNA expression of 10 genes was analyzed by real time RT-PCR. It shows that the mRNA expression of talin-1, vinculin and coronin-1C were up-regulated in HIV positive patients and consistent with protein expression. Western blotting analysis confirmed the induction of fragments of vinculin, talin-1 and filamin-A in pooled and most part of individual HIV-positive clinical samples. Bioinformatic analysis showed that a wide host protein network was disrupted in HIV-positive patients.


CONCLUSIONS
Together, this work provided useful information to facilitate further investigation of the underlying mechanism of HIV and host cell protein interactions, and discovered novel potential biomarkers such as fragment of vinculin, filamin-A and talin-1 for anti-HIV research.
Zhang
Lijun
L
Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Jia
Xiaofang
X
Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Zhang
Xiaojun
X
Neurosurgery, Fuzhou General Hospital, Fuzhou 350025, PR China.
Sun
Jianjun
J
Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Peng
Xia
X
Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Qi
Tangkai
T
Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Ma
Fang
F
Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Yin
Lin
L
Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Yao
Yamin
Y
Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Qiu
Chao
C
Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, PR China.
Lu
Hongzhou
H
Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.