Searching for monooxygenases and hydrolases in bacteria from an extreme environment.
Microbial oxidation potentials of extremophiles recovered from Pampo Sul oil field, Campos Basin, Brazil, in pure culture or in consortia, were investigated using high-throughput screening (HTS) and multibioreactions. Camphor (1), cis-jasmone (2), 2-methyl-cyclohexanone (3), 1,2-epoxyoctane (4), phenylethyl acetate (5), phenylethyl propionate (6), and phenylethyl octanoate (7) were used to perform multibioreaction assays. Eighty-two bacterial isolates were recovered from oil and formation water samples and those presenting outstanding activities in HTS assays were identified by sequencing their 16S rRNA genes. These results revealed that most microorganisms belonged to the genus Bacillus and presented alcohol dehydrogenase, monooxygenase, epoxide hydrolase, esterase, and lipase activities.
da Cruz
Georgiana F
GF
Chemistry Institute, University of Campinas, UNICAMP, P.O. Box 6154, 13084-971 Campinas, SP, Brazil.
Angolini
Célio F F
CF
de Oliveira
Luciana G
LG
Lopes
Patrícia F
PF
de Vasconcellos
Suzan P
SP
Crespim
Elaine
E
de Oliveira
Valéria M
VM
dos Santos Neto
Eugênio V
EV
Marsaioli
Anita J
AJ
D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D006899:Mixed Function Oxygenases; D006867:Hydrolases