[Protective effect of Xinaoning freezedrying power (XNN) against global cerebral ischemia-reperfusion injury].
OBJECTIVE
To study the protective effects of Xinaoning freezedrying power (XNN) against global cerebral ischemia-reperfusion injury.


METHODS
Pulsinelli four-vessel occlusion method was used to make global cerebral ischemia-reperfusion model of rats. The EEG and the reappearing time of righting reflex were recorded, and the activity of SOD, LDH, NOS and the content of MDA and LD in the brain were tested. The ET content in hippocampi was researched by radioimmunoassay method. Aggregation of platelets induced by ADP was examined. The resting and free intracellular calcium concentration ([Ca2+]i) in platelets induced by CaCl2 was measured by double wavelength fluorescence sepectrophotometer with a Ca2+ sensitive fluorescent indicator (Furo-2).


RESULTS
XNN could promote the recovery of EEG and righting reflex, reduce the brain edema and brain index, increase SOD and LDH activities, inhibit NOS activity, decrease MDA, LD and ET contents, inhibit platelet aggregation, reduce the resting [Ca2+]i and inhibit the increase of [Ca2+]i induced by CaCl2 in rats.


CONCLUSIONS
XNN has protective effect against golobal cerebral ischemai-reperfusion injury.
Yin
Yanyan
Y
Department of Pharmacology, Anhui Medical University, Hefei 230032, China. yinyanyan5678@126.com
Cao
Xi
X
Li
Weizu
W
D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D009569:Nitric Oxide; D002118:Calcium