[First description of phenotypic profile and in vitro drug susceptibility of Cryptococcus spp yeast isolated from HIV-positive and HIV-negative patients in State of Mato Grosso].
Thirty-seven isolates from 10 HIV-negative and 26 HIV-positive patients in Mato Grosso were evaluated. Direct examination, culturing and chemotyping of species were performed. Ketoconazole, itraconazole, voriconazole, fluconazole and amphotericin B were evaluated. Thirty-seven yeasts of Cryptococcus spp were identified, of which 26 were from HIV-positive patients (25 Cryptococcus neoformans and one Cryptococcus gattii) and 10 from HIV-negative patients (five Cryptococcus neoformans and five Cryptococcus gattii). The Cryptococcus neoformans clinical isolates from HIV-positive patients showed resistance (8% and 8.7%) and dose-dependent susceptibility (20% and 17.4%) to fluconazole and itraconazole, respectively. Among the Cryptococcus neoformans isolates from HIV-negative patients, there was dose-dependent susceptibility (40%) to fluconazole. Cryptococcus gattii isolates from HIV-negative patients were shown to be susceptible to all antifungal agents, except for one isolate of Cryptococcus gattii that showed dose-dependent susceptibility to fluconazole (20%). The Cryptococcus gattii isolate from an HIV-positive patient showed resistance to fluconazole (MIC > or = 256 (1/4)g/ml) and itraconazole (MIC = 3 (1/4)microg/ml).
Favalessa
Olivia Cometti
OC
Laboratório de Investigação (Micologia), Faculdade de Ciências Médicas, Universidade Federal de Mato Grosso, Cuiabá, MT.
Ribeiro
Luciano Correa
LC
Tadano
Tomoko
T
Fontes
Cor Jesus Fernandes
CJ
Dias
Flávio Basili
FB
Coelho
Bruno Pereira Albuquerque
BP
Hahn
Rosane Christine
RC
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology*; D000328:Adult; D000935:Antifungal Agents / Q000494:pharmacology*; D056285:Cryptococcus gattii / Q000187:drug effects* / Q000235:genetics; D003455:Cryptococcus neoformans / Q000187:drug effects* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D011446:Prospective Studies
D000935:Antifungal Agents