Chemical constituents of Alnus firma and their inhibitory activity on lipopolysaccharide-induced nitric oxide production in BV2 microglia.
A methanolic extract of ALNUS FIRMA barks (Betulaceae) significantly attenuated nitric oxide production in lipopolysaccharide-stimulated BV2 microglia. Two new compounds, characterized as 4-hydroxy-2,6-dimethoxyphenyl 6'- O-syringoyl- beta- D-glucopyranoside (1) and 4-hydroxy-2,6-dimethoxyphenyl 6'- O-vanilloyl- beta- D-glucopyranoside (2), were isolated from the barks of A. FIRMA using bioactivity-guided fractionation, together with two known phenolic glycosides (3, 4) and 11 known diarylheptanoids (5- 15). Among these compounds, 1- 4 and 6- 11 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglial cells at concentrations ranging from 10 microM to 100 microM.
Lee
Min A
MA
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
Lee
Hee Kyoung
HK
Kim
Seung Hyun
SH
Kim
Young Choong
YC
Sung
Sang Hyun
SH
D029661:Alnus / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D036381:Diarylheptanoids / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
C555504:4-hydroxy-2,6-dimethoxyphenyl-6'-O-syringoyl-beta-D-glucopyranoside; C555505:4-hydroxy-2,6-dimethoxyphenyl-6'-O-vanilloyl-beta-D-glucopyranoside; D000893:Anti-Inflammatory Agents; D036381:Diarylheptanoids; D006027:Glycosides; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide