A simple, sensitive determination of ganciclovir in infant plasma by high-performance liquid chromatography with fluorescence detection.
This study developed a simple and sensitive method using reversed-phase high-performance liquid chromatography (HPLC) for ganciclovir (GCV) plasma concentrations in cytomegalovirus infectious infants with hearing loss. The method involves a simple protein precipitation procedure that uses no solid-phase or liquid-liquid extraction. The HPLC separation was carried out on a Cadenza CD-C(18) column (3 microm, 4.6 mm x 150 mm) with phosphate buffer (pH 2.5, 25 mM) containing 1% methanol-acetonitrile mixture (4:3, v/v) as a mobile phase at a 0.7 mL/min flow rate. GCV was detected using a fluorescence detection (lambdaex/em: 265/380 nm). The quantification limit was 0.025 microg/mL for 100 microL of plasma sample at which good intra- and inter-assay coefficient of variation values (< 4.96%) and recoveries (94.9-96.5%) were established.
Yoshida
Terumitsu
T
Saitama Children's Medical Center, Division of Clinical Research and Department of Pharmacy, Japan. a0183776@pref.saitama.lg.jp
Takahashi
Ryohei
R
Imai
Koichi
K
Uchida
Hiroshi
H
Arai
Yasutoshi
Y
Oh-ishi
Tsutomu
T
D000998:Antiviral Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000191:economics / Q000379:methods*; D005453:Fluorescence; D015774:Ganciclovir / Q000097:blood*; D006801:Humans; D007223:Infant; D057230:Limit of Detection; D016014:Linear Models
D000998:Antiviral Agents; D015774:Ganciclovir