title: [Complete sequence of the M segment of Amur virus in rodent from China].
abstract: OBJECTIVE
  To study the complete sequence of M segment of Amur virus in rodents and to explore their molecular characteristics.
  
  
  METHODS
  Complete M segment of Amur virus in rodent from China was amplified by RT-PCR. The purified PCR product was cloned into pGEM-T Easy vector and then sequenced. Phylogenetic analysis on multiple nucleotide sequences was performed with the Tree PUZZLE and DNAStar software.
  
  
  RESULTS
  The full-length of its M gene comprised of 3615 nucleotides with one open reading frame (ORF) including 3408 nucleotides and encoding a protein which comprised 1135 amino acids. The ORF was located at bases 41 to 3448. The phylogenetic analysis of JilinAP06 with other hantaviruses revealed that the complete sequence of M segment of JilinAP06 strain was closely related to those Amur viruses such as B78 strain, Liu strain and H5 strain were all from the patients. The complete sequence of M segment of JilinAP06 had only 79.5% identities with the nucleotide sequence of HTNV strain 76 - 118.
  
  
  CONCLUSIONS
  The complete sequence on M segment of Amur virus in rodent was first time identified in this country.
authors.affiliation: Inner Mongolia Autonomous Region Center for Disease Control and Prevention, Huhehaote 010031, China.
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Guo
authors.forename: Wen-yi
authors.initials: WY
authors.lastname: Zhang
authors.forename: Jia-fu
authors.initials: JF
authors.lastname: Jiang
authors.forename: Hai-yi
authors.initials: HY
authors.lastname: Chi
authors.forename: Wen-rui
authors.initials: WR
authors.lastname: Wang
authors.forename: Zhong-yuan
authors.initials: ZY
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Jin
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Li-qun
authors.initials: LQ
authors.lastname: Fang
authors.forename: Wu-chun
authors.initials: WC
authors.lastname: Cao
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D006476:Orthohantavirus / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012377:Rodentia / Q000821:virology*