title: [Examination of cause of failure to taxon-specific DNA was not detected from bifun (rice vermicelli) using processing model experiment].
abstract: In the inspection of genetically modified rice, rice taxon-specific DNA could not be detected in processed rice food (bifun: rice vermicelli). The effects of using PCR the ratio of rice powder content and temperature of processing on the detection of taxon-specific DNA were examined by means of processing model experiments using cornstarch with 0, 2, 5, 10% rice powder by weight. Cornstarch and rice powder were blended with water and subjected to heating, steam-treatment, and autoclaving. The rice taxon-specific DNA was detectable in 2% rice powder following heat and steam treatments. After autoclaving, rice taxon-specific DNA was detected only in the 10% rice powder product. In the processing model experiment using rice powder, it was found that autoclaving caused severe DNA degradation.
authors.affiliation: Saitama Institute of Public Health, 639-1 Kamiokubo, Sakura-ku, Saitama-shi, Saitama 338-0824, Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Takahashi
authors.forename: Rie
authors.initials: R
authors.lastname: Ishii
authors.forename: Ryuji
authors.initials: R
authors.lastname: Matsumoto
authors.forename: Masakazu
authors.initials: M
authors.lastname: Horie
mesh_terms: D004247:DNA / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling; D030841:Food, Genetically Modified; D006358:Hot Temperature; D012275:Oryza / Q000737:chemistry* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004247:DNA