Impact of siderophore production by Pseudomonas syringae pv. syringae 22d/93 on epiphytic fitness and biocontrol activity against Pseudomonas syringae pv. glycinea 1a/96.
The use of naturally occurring microbial antagonists to suppress plant diseases offers a favorable alternative to classical methods of plant protection. The soybean epiphyte Pseudomonas syringae pv. syringae strain 22d/93 shows great potential for controlling P. syringae pv. glycinea, the causal agent of bacterial blight of soybean. Its activity against P. syringae pv. glycinea is highly reproducible even in field trials, and the suppression mechanisms involved are of special interest. In this work we demonstrated that P. syringae pv. syringae 22d/93 produced a significantly larger amount of siderophores than the pathogen P. syringae pv. glycinea produced. While P. syringae pv. syringae 22d/93 and P. syringae pv. glycinea produce the same siderophores, achromobactin and pyoverdin, the regulation of siderophore biosynthesis in the former organism is very different from that in the latter organism. The epiphytic fitness of P. syringae pv. syringae 22d/93 mutants defective in siderophore biosynthesis was determined following spray inoculation of soybean leaves. The population size of the siderophore-negative mutant P. syringae pv. syringae strain 22d/93DeltaSid was 2 orders of magnitude lower than that of the wild type 10 days after inoculation. The growth deficiency was compensated for when wound inoculation was used, indicating the availability of iron in the presence of small lesions on the leaves. Our results suggest that siderophore production has an indirect effect on the biocontrol activity of P. syringae pv. syringae 22d/93. Although siderophore-defective mutants of P. syringae pv. syringae 22d/93 still suppressed development of bacterial blight caused by P. syringae pv. glycinea, siderophore production enhanced the epiphytic fitness and thus the competitiveness of the antagonist.
Wensing
Annette
A
School of Engineering and Science, Jacobs University Bremen, Bremen, Germany.
Braun
Sascha D
SD
Büttner
Petra
P
Expert
Dominique
D
Völksch
Beate
B
Ullrich
Matthias S
MS
Weingart
Helge
H
D000898:Antibiosis; D002951:Citrates / Q000096:biosynthesis; D007656:Ketoglutaric Acids; D009842:Oligopeptides / Q000096:biosynthesis; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D044224:Pseudomonas syringae / Q000378:metabolism*; D017262:Siderophores / Q000096:biosynthesis*; D013025:Glycine max / Q000382:microbiology
D002951:Citrates; D007656:Ketoglutaric Acids; D009842:Oligopeptides; D017262:Siderophores; C413577:achromobactin; C042453:pyoverdin