title: A rat monoclonal antibody against the chromatin remodeling factor CHD5.
abstract: CHD5 (chromodomain/helicase/DNA-binding protein 5) is a member of the CHD subfamily of chromatin remodeling Swi/Snf proteins, and has been recently identified as a tumor suppressor in a diverse range of human cancers. We report here on the establishment of a hybridoma cell line for producing a monoclonal antibody against CHD5 by the rat medial iliac lymph node method. Immunoblotting analyses indicated that this antibody, MAb 5A10, specifically recognizes endogenous CHD5. In immunostaining using the antibody, a nuclear staining pattern was observed. The monoclonal antibody will be useful in immunoblotting and immunolocalization experiments in a variety of cells and tissues, as well as in further studies of the biological function and cellular dynamics of this protein.
authors.affiliation: Department of Bioengineering, Graduate School of Engineering, Osaka City University, Osaka, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Yoshimura
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Yoshimi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ohkawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Azuma
authors.forename: Taro
authors.initials: T
authors.lastname: Tachibana
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D004265:DNA Helicases / Q000235:genetics / Q000276:immunology*; D004622:Embryo, Mammalian / Q000166:cytology / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007082:Ileum / Q000276:immunology; D007114:Immunization; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000276:immunology*; D051381:Rats; D011921:Rats, Inbred WKY; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D004265:DNA Helicases; C471926:CHD5 protein, human; C572553:CHD5 protein, rat