title: Analysis of effect of nanoporous alumina substrate coated with polypyrrole nanowire on cell morphology based on AFM topography.
abstract: In this study, in situ electrochemical synthesis of polypyrrole nanowires with nanoporous alumina template was described. The formation of highly ordered porous alumina substrate was demonstrated with Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM). In addition, Fourier transform infrared analysis confirmed that polypyrrole (PP) nanowires were synthesized by direct electrochemical oxidation of pyrrole. HeLa cancer cells and HMCF normal cells were immobilized on the polypyrrole nanowires/nanoporous alumina substrates to determine the effects of the substrate on the cell morphology, adhesion and proliferation as well as the biocompatibility of the substrate. Cell adhesion and proliferation were characterized using a standard MTT assay. The effects of the polypyrrole nanowires/nanoporous alumina substrate on the cell morphology were studied by AFM. The nanoporous alumina coated with polypyrrole nanowires was found to exhibit better cell adhesion and proliferation than polystyrene petridish, aluminum foil, 1st anodized and uncoated 2nd anodized alumina substrate. This study showed the potential of the polypyrrole nanowires/nanoporous alumina substrate as biocompatibility electroactive polymer substrate for both healthy and cancer cell cultures applications.
authors.affiliation: Department of Interdisciplinary program of Integrated Biotechnology, Sogang University, Seoul, Korea.
authors.forename: Waleed Ahmed
authors.initials: WA
authors.lastname: El-Said
authors.forename: Cheol-Heon
authors.initials: CH
authors.lastname: Yea
authors.forename: Mi
authors.initials: M
authors.lastname: Jung
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Kim
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D006367:HeLa Cells; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D053770:Nanowires / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry
chemical_list: D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D000537:Aluminum Oxide