title: PCR/LDR/universal array platforms for the diagnosis of infectious disease.
abstract: Infectious diseases account for between 14 and 17 million deaths worldwide each year. Accurate and rapid diagnosis of bacterial, fungal, viral, and parasitic infections is therefore essential to reduce the morbidity and mortality associated with these diseases. Classical microbiological and serological methods have long served as the gold standard for diagnosis but are increasingly being replaced by molecular diagnostic methods that demonstrate increased sensitivity and specificity and provide an identification of the etiologic agent in a shorter period of time. PCR/LDR coupled with universal array detection provides a highly sensitive and specific platform for the detection and identification of bacterial and viral infections.
authors.affiliation: Department of Microbiology, Weill Medical College, Cornell University, New York, NY, USA. mrp2003@med.cornell.edu
authors.forename: Maneesh
authors.initials: M
authors.lastname: Pingle
authors.forename: Mark
authors.initials: M
authors.lastname: Rundell
authors.forename: Sanchita
authors.initials: S
authors.lastname: Das
authors.forename: Linnie M
authors.initials: LM
authors.lastname: Golightly
authors.forename: Francis
authors.initials: F
authors.lastname: Barany
mesh_terms: D003141:Communicable Diseases / Q000175:diagnosis* / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D008025:Ligases / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019095:Point-of-Care Systems; D016133:Polymerase Chain Reaction / Q000379:methods*; D011327:Printing; D011786:Quality Control
chemical_list: D009841:Oligonucleotides; D008025:Ligases