title: Evidence that the C-terminus of OprM is involved in the assembly of the VceAB-OprM efflux pump.
abstract: Although the architecture of tripartite multiple drug resistance (MDR) efflux pumps of Gram-negative bacteria has been well characterized, the means by which the components recognize each other and assemble into a functional pump remains obscure. In this study we present evidence that the C-terminal domain of the Pseudomonas aeruginosa OprM and the alpha-helical hairpin domain of Vibrio cholerae VceA play an important role in the recognition/specificity/recruitment step in the assembly of a functional, VceAB-OprM chimeric efflux pump. To our knowledge, this is the first evidence directly linking the C-terminal domain of an outer membrane efflux protein to its recruitment during the assembly of a tripartite efflux pump.
authors.affiliation: Department of Microbiology and Immunology, Texas Tech University Health Science Center, Lubbock, TX 79430, USA.
authors.forename: Jiangping
authors.initials: J
authors.lastname: Bai
authors.forename: Lakysha
authors.initials: L
authors.lastname: Mosley
authors.forename: Joe A
authors.initials: JA
authors.lastname: Fralick
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D019450:Genes, MDR; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa; D014734:Vibrio cholerae
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C402263:OprM protein, Pseudomonas aeruginosa; C489682:VceA protein, Vibrio cholerae; C077169:VceB protein, Vibrio cholerae