8-Quinolineboronic acid as a potential phosphorescent molecular switch for the determination of alpha-fetoprotein variant for the prediction of primary hepatocellular carcinoma.
8-Quinolineboronic acid phosphorescent molecular switch (8-QBA-PMS) in the "off" state emitted weak room temperature phosphorescence (RTP) of 8-QBA on the acetylcellulose membrane (ACM) with the perturbation of Pb(2+). When 8-QBA-PMS was used to label concanavalin agglutinin (Con A) to form 8-QBA-PMS-Con A based on the reaction between -OH of 8-QBA-PMS and -COOH of Con A, 8-QBA-PMS turned "on" automatically due to its structure change, and RTP of the system increased 2.7 times. Besides, -NH(2) of 8-QBA-PMS-Con A could carry out affinity adsorption (AA) reaction with the -COOH of alpha-fetoprotein variant (AFP-V) to form the product Con A-AFP-V-Con A-8-QBA-PMS containing -NH-CO- bond, causing the RTP of the system to further increase. Moreover, the amount of AFP-V was linear to the DeltaI(p) of the system in the range of 0.012-2.40 (fg spot(-1)). Thus, a new affinity sensitive adsorption solid substrate room temperature phosphorimetry using 8-QBA-PMS as labelling reagent (8-QBA-PMS-AASSRTP) for the determination of AFP-V was proposed with the detection limit (LD) of 9 x 10(-15) g mL(-1). It had been used to determine AFP-V in human serum with the results agreeing with enzyme-link immunoassay (ELISA), showing promise for the prediction of PHC due to the intimate association between AFP-V and primary hepatocellular carcinoma (PHC). The mechanism of the promethod was also discussed.
Liu
Jia-Ming
JM
Department of Chemistry and Environmental Science, Zhangzhou Normal College, Xianzhiqian Street, 36# Zhangzhou, Fujian 363000, PR China. zzsyliujiaming@163.com
Li
Fei-Ming
FM
Liu
Zhen-Bo
ZB
Lin
Chang-Qing
CQ
Lin
Shao-Qin
SQ
Lin
Li-Ping
LP
Wang
Xin-Xing
XX
Li
Zhi-Ming
ZM
D001897:Boronic Acids / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000737:chemistry*; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements; D020033:Protein Isoforms / Q000097:blood; D011804:Quinolines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013696:Temperature; D000509:alpha-Fetoproteins / Q000032:analysis*
D001897:Boronic Acids; D049408:Luminescent Agents; D020033:Protein Isoforms; D011804:Quinolines; D000509:alpha-Fetoproteins; C477948:8-quinolineboronic acid