title: A role for the C terminus of Mopeia virus nucleoprotein in its incorporation into Z protein-induced virus-like particles.
abstract: Arenaviruses are enveloped, negative-strand RNA viruses. For several arenaviruses, virus-like particle (VLP) formation requires the viral matrix Z protein. However, the mechanism by which viral ribonucleoprotein complexes are incorporated into virions is poorly understood. Here, we show that the expression of the Z protein and nucleoprotein (NP) of Mopeia virus, a close relative of the pathogenic Lassa virus, resulted in the highly selective incorporation of the NP protein into Z protein-induced VLPs. Moreover, the Z protein promoted the association of NP with cellular membranes, suggesting that the association of NP, Z, and the cellular membranes may facilitate the efficient incorporation of NP into VLPs. By employing a series of NP deletion constructs and testing their VLP incorporation, we further demonstrated an important role for the C-terminal half of NP in its incorporation into VLPs.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 575 Science Drive, Madison, WI 53711, USA.
authors.forename: Olena
authors.initials: O
authors.lastname: Shtanko
authors.forename: Masaki
authors.initials: M
authors.lastname: Imai
authors.forename: Hideo
authors.initials: H
authors.lastname: Goto
authors.forename: Igor S
authors.initials: IS
authors.lastname: Lukashevich
authors.forename: Gabriele
authors.initials: G
authors.lastname: Neumann
authors.forename: Tokiko
authors.initials: T
authors.lastname: Watanabe
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
mesh_terms: D000818:Animals; D018049:Arenaviruses, Old World / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D006801:Humans; D009698:Nucleoproteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D017384:Sequence Deletion; D014763:Viral Matrix Proteins / Q000378:metabolism*; D022701:Virosomes / Q000378:metabolism; D019065:Virus Assembly
chemical_list: D009698:Nucleoproteins; D014763:Viral Matrix Proteins; D022701:Virosomes