Omics-based analysis of the luxS mutation in a clinical isolate of Escherichia coli O157:H7 in Korea.
The purpose of this study was to investigate the relationship between the global regulatory mechanism known as quorum sensing and expression of virulence factors in Escherichia coli O157:H7. A non-polar luxS deletion was introduced into the chromosome of strain CI03J, a human clinical isolate from South Korea, to create the DeltaluxS mutant strain ML03J. Phenotypic characterization of wild-type and mutant strains demonstrated that ML03J had no obvious growth or metabolic defects on 0.2% glucose LB medium, produced a functionally-defective flagellum, and could not utilize sorbose; the biological significance of sorbose utilization is unknown. Omics-based analysis revealed the involvement of LuxS in the transcriptional activation of several flagella/chemotaxis-related genes (flhD; fliA, C, D, S, Z; cheA, Y, and Z), repression of glutamate-dependent acid resistance genes (gadAB), and expression of virulence factors including Shiga toxin, hemolysin, and SepD within the LEE pathogenicity island.
Kim
Jong-Chul
JC
Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health Seoul 122-701, Korea.
Yoon
Jang Won
JW
Kim
Jong-Bae
JB
Oh
Kyung-Hwan
KH
Park
Mi-Sun
MS
Lee
Bok-Kwon
BK
Cho
Seung-Hak
SH
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D013437:Carbon-Sulfur Lyases / Q000235:genetics* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D009154:Mutation; D053038:Quorum Sensing; D056910:Republic of Korea; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D037521:Virulence Factors; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria