[Analysis of single oocytes and follicles from surgically recovered ovarian tissue of patients with ovarian polycystosis].
Our study included material of 30 patients with ovarian polycystosis (PCOS-polycystic ovary syndrome). Informed consent was obtained from patients before their laparoscopic surgery and biopsy. A total of 16 oocytes and small follicles were isolated from (33.33%) of cases by stereomicroscopic dissection. This was followed by follicle cells removing, zona pellucida dissolving, hypotonic incubation and fixation onto slides. On the fixed samples, FISH with markers for chromosomes 18, 21 and X was performed. Analysis was successful in 11 (68.75%) of objects (5 follicles and 6 oocytes). Five oocytes (31.25%) had a negative FISH reaction because of condensed chromatin. This indicated cellular ageing which is characteristic of atretic follicles.


CONCLUSIONS
(1) We obtained data about chromatin and chromosomal status of analysed oocytes, which correlated with ovarian reserve dynamics in PCOS and the processes of maturation and chromatin ageing in growing and atretic follicles. (2) The study has provided information about biological parameters of ovarian tissue in PCO that could be useful for obtaining precise patients' diagnosis and for their reproductive prognosis. (3) The problem needs more extensive studies because the number of analyzed oocytes was insufficient to allow data comparison for aneuploidy in PCOS ovaries vs. normally functioning ovaries.
D002843:Chromatin / Q000473:pathology; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D010535:Laparoscopy; D009865:Oocytes / Q000473:pathology*; D006080:Ovarian Follicle / Q000473:pathology*; D010053:Ovary / Q000473:pathology / Q000601:surgery*; D011085:Polycystic Ovary Syndrome / Q000175:diagnosis / Q000473:pathology* / Q000601:surgery*
D002843:Chromatin