title: Development of an insect cell-free system.
abstract: Cell-free protein synthesis systems offer production of native proteins with high speed, even for the proteins that are toxic to cells. Among cell-free systems, the system derived from insect cells has the potential to carry out post-translational modifications that are specific to eukaryotic organisms, as occurs in the rabbit reticulocyte system. In this review, we describe development of this insect cell-free system and its applications.
authors.affiliation: Clinical and Biotechnology Business Unit, Life Science Business Department, Analytical and Measuring Instruments Division, Shimadzu Corporation, 1 Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan. ezure@shimadzu.co.jp
authors.forename: Toru
authors.initials: T
authors.lastname: Ezure
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Shikata
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ito
authors.forename: Eiji
authors.initials: E
authors.lastname: Ando
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Utsumi
authors.forename: Osamu
authors.initials: O
authors.lastname: Nishimura
authors.forename: Susumu
authors.initials: S
authors.lastname: Tsunasawa
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002474:Cell-Free System / Q000737:chemistry* / Q000378:metabolism*; D007313:Insecta / Q000737:chemistry* / Q000378:metabolism*; D015202:Protein Engineering / Q000639:trends*; D011817:Rabbits; D011994:Recombinant Proteins / Q000138:chemical synthesis* / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins