title: Conversion of squid pen by Pseudomonas aeruginosa K187 fermentation for the production of N-acetyl chitooligosaccharides and biofertilizers.
abstract: Pseudomonas aeruginosa K187, a protease- and chitinase-producing bacterium, exhibited protease and chitinase activity after three and five days of incubation, respectively. The protease and chitinase were both produced by using 1% squid pen powder (SPP) (w/v) as sole carbon and nitrogen source. After fermentation, the deproteinization rate of the recovered squid pen gradually increased up to 68% on the fourth day. After five days of fermentation, the production of GlcNAc, (GlcNAc)(2), (GlcNAc)(3), (GlcNAc)(4) and (GlcNAc)(5) were 1.18mg/mL, 0.76mg/mL, 1.02mg/mL, 0.93mg/mL and 0.90mg/mL, respectively. The culture supernatant of K187 also exhibited activity of enhancing vegetable growth. For Brassica chinensis Linn treated with the fifth day culture supernatant, the total weight and total length increased up to 529% and 148%, respectively, compared to the control group. With this method, the production of protease, chitinase, N-acetyl chitooligosaccharides and biofertilizers may be useful for biological applications.
authors.affiliation: Life Sciences Development Center, Tamkang University, Taipei 25137, Taiwan. sabulo@mail.tku.edu.tw <sabulo@mail.tku.edu.tw>
authors.forename: San-Lang
authors.initials: SL
authors.lastname: Wang
authors.forename: Wan-Han
authors.initials: WH
authors.lastname: Hsu
authors.forename: Tzu-Wen
authors.initials: TW
authors.lastname: Liang
mesh_terms: D000818:Animals; D001937:Brassica / Q000187:drug effects / Q000254:growth & development; D002688:Chitinases / Q000378:metabolism; D049832:Decapodiformes; D005285:Fermentation; D005308:Fertilizers / Q000032:analysis; D017744:Medical Waste Disposal / Q000379:methods*; D009844:Oligosaccharides / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005308:Fertilizers; D017744:Medical Waste Disposal; D009844:Oligosaccharides; D002688:Chitinases; D010447:Peptide Hydrolases