CE-TOF MS analysis of complex protein hydrolyzates from genetically modified soybeans--a tool for foodomics.
A CE-TOF MS proteomic approach was applied for the analysis of hydrolyzates from complex soybean protein mixtures. After CE-TOF MS method development, the new approach provided the simultaneous analysis of more than 150 peptides from the soybean protein fraction soluble in ACN-water (80/20 v/v). The method is fast (about 30 min of analysis per sample) and is characterized by a relatively low running cost. The approach was used to study the substantial equivalence between a genetically modified variety of soybean compared with its traditional counterpart. No significant differences were found between the two studied soybeans based on the protein fraction studied. The capacity of the CE-TOF MS method to analyze complex mixtures of peptides in short times opens interesting possibilities in the growing Foodomics area.
Simó
Carolina
C
Department of Food Analysis, Institute of Industrial Fermentations, CSIC, Madrid, Spain.
Domínguez-Vega
Elena
E
Department of Analytical Chemistry, Faculty of Chemistry, University of Alcalá, Madrid, Spain.
Marina
Maria Luisa
ML
Department of Analytical Chemistry, Faculty of Chemistry, University of Alcalá, Madrid, Spain.
García
María Concepción
MC
Department of Analytical Chemistry, Faculty of Chemistry, University of Alcalá, Madrid, Spain.
Dinelli
Giovanni
G
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy.
Cifuentes
Alejandro
A
Department of Food Analysis, Institute of Industrial Fermentations, CSIC, Madrid, Spain.
D000097:Acetonitriles / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005504:Food Analysis / Q000379:methods*; D006868:Hydrolysis; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry* / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
D000097:Acetonitriles; D010446:Peptide Fragments; D010940:Plant Proteins; D014357:Trypsin; C032159:acetonitrile