The protective effect of bee venom against ethanol-induced hepatic injury via regulation of the mitochondria-related apoptotic pathway.
Alcohol consumption increases apoptosis of hepatocytes. Death of hepatocytes is a characteristic feature of chronic liver disease for various causes. Bee venom (Apis mellifera) has been traditionally used for the treatment of various chronic diseases, such as chronic inflammatory arthritis and chronic liver disease. However, the precise mechanism for bee venom in chronic liver disease is not still cleared. To assess the effects of bee venom in chronic liver disease, we investigated the potential role of the bee venom in the ethanol-induced hepatocyte apoptosis. Bee venom treatment inhibited the apoptotic cell morphology and increased the cell viability in ethanol-induced hepatocyte apoptosis. With ethanol treatment, bee venom-treated hepatocytes increased activity of Bcl-2 and Bcl-xL, reduced activity of Bax, Caspase and PARP. In conclusion, bee venom treatment in ethanol-induced hepatocyte apoptosis occurred through the regulation of Bcl family with subsequent inactivation of the Caspase and PARP. These results suggest that bee venom could be an effective agent to reduce ethanol-induced hepatocyte apoptosis.
Kim
Kyung-Hyun
KH
Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, Republic of Korea.
Kum
Yoon-Seup
YS
Park
Yoon-Yup
YY
Park
Ji-Hyun
JH
Kim
Soo-Jung
SJ
Lee
Woo-Ram
WR
Lee
Kwang-Gil
KG
Han
Sang-Mi
SM
Park
Kwan-Kyu
KK
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001514:Bee Venoms / Q000494:pharmacology*; D061945:Caspase Inhibitors; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects; D004336:Drug Antagonism; D000431:Ethanol / Q000633:toxicity*; D019084:Fluorescent Antibody Technique, Indirect; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D020011:Protective Agents / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000037:antagonists & inhibitors / Q000378:metabolism
C494828:Bax protein, rat; D001514:Bee Venoms; D061945:Caspase Inhibitors; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D020011:Protective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D000431:Ethanol