title: miR-24 regulates apoptosis by targeting the open reading frame (ORF) region of FAF1 in cancer cells.
abstract: BACKGROUND
  microRNAs (miRNAs) are small noncoding RNAs that regulate cognate mRNAs at the post-transcriptional stage. Several studies have shown that miRNAs modulate gene expression in mammalian cells by base pairing to complementary sites in the 3'-untranslated region (3'-UTR) of the target mRNAs.
  
  
  RESULTS
  In the present study, miR-24 was found to target fas associated factor 1(FAF1) by binding to its amino acid coding sequence (CDS) region, thereby regulating apoptosis in DU-145 cells. This result supports an augmented model whereby animal miRNAs can exercise their effects through binding to the CDS region of the target mRNA. Transfection of miR-24 antisense oligonucleotide (miR-24-ASO) also induced apoptosis in HGC-27, MGC-803 and HeLa cells.
  
  
  CONCLUSIONS
  We found that miR-24 regulates apoptosis by targeting FAF1 in cancer cells. These findings suggest that miR-24 could be an effective drug target for treatment of hormone-insensitive prostate cancer or other types of cancers. Future work may further develop miR-24 for therapeutic applications in cancer biology.
authors.affiliation: State Key Laboratory of Molecular Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Wenming
authors.initials: W
authors.lastname: Qin
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
authors.forename: Botao
authors.initials: B
authors.lastname: Zhao
authors.forename: Chengguo
authors.initials: C
authors.lastname: Yao
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.forename: Jiexian
authors.initials: J
authors.lastname: Ma
authors.forename: Youxin
authors.initials: Y
authors.lastname: Jin
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D016376:Oligonucleotides, Antisense / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics*; D011485:Protein Binding; D019311:Staurosporine / Q000494:pharmacology; D014162:Transfection
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D004791:Enzyme Inhibitors; C490780:FAF1 protein, human; C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D019311:Staurosporine