The enhancement effect of beta-boswellic acid on hippocampal neurites outgrowth and branching (an in vitro study).
Increasing evidences implicate impairment of axonal integrity in mechanisms underlying neurodegenerative disorders. Beta-boswellic acid (BBA) is the major component of Boswellia serrata gum. This resin has long been used in Ayurveda (India's traditional medicine) to prevent amnesia. In this study, the effect of BBA was examined on neurites outgrowth and branching as well as on polymerization dynamics of tubulin. The morphometric parameters (axonal length and neuritis branching) were examined microscopically after treating the hippocampal cells with BBA. Also the assembly process of tubulin was assessed using UV/V is spectrophotometer through following of absorbance at 350 nm. The results revealed that BBA could significantly enhance neurite outgrowth, branching, and tubulin polymerization dynamics. The obtained results suggest that enhancing effect of BBA on microtubule polymerization kinetics might be the origin of increasing axonal outgrowth and branching.
Karima
Oveis
O
Institute of Biochemistry and Biophysics, University of Tehran, Enghelab Avenue, 14155-6461, Tehran, Iran.
Riazi
Gholamhossein
G
Yousefi
Reza
R
Movahedi
Ali Akbar Moosavi
AA
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology; D049108:Cell Enlargement / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D007700:Kinetics; D008870:Microtubules / Q000378:metabolism; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018697:Nootropic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014315:Triterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D014404:Tubulin / Q000378:metabolism
D018697:Nootropic Agents; D014315:Triterpenes; D014404:Tubulin; C054625:boswellic acid