title: [Analyzing secondary structure of beta2 microglobulin in Escherichia coli with circular dichroism spectrum].
abstract: OBJECTIVE
  In order to study the structure and function of beta2 microglobulin (beta2 m).
  
  
  METHODS
  We sub-cloned the mature peptide of beta2 m into the p2X plasmid and transformed them to Escherichia coli TB1. The recombinant bacterium was induced to be expressed and the expressed fusion protein was detected by SDS-PAGE and western blot. After purifying and cleaving with Factor Xa, we separated the monomer protein beta2 m from MBP. Finally, we determined the secondary structure of the beta2 m protein by circular dichroism (CD) spectrum.
  
  
  RESULTS
  MBP-beta2 m was 52.1 kDa, and the monomer protein beta2 m was 10.6 kDa. The alpha-helix, beta-sheet, turn, and random coil of the fusion protein showed 0, 45, 8 and 45 amino acids, respectively, detected by CD estimation.
  
  
  CONCLUSIONS
  The beta2 m protein had correct secondary structure and could be used for further research of peptide binding in vitro.
authors.affiliation: Dalian University, Bioengineering College, Dalian 116622, China. gfsh0626@126.com
authors.forename: Fengshan
authors.initials: F
authors.lastname: Gao
authors.forename: Chun
authors.initials: C
authors.lastname: Xia
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yungang
authors.initials: Y
authors.lastname: Li
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Li
authors.forename: Chongbo
authors.initials: C
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D008970:Molecular Weight; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013552:Swine; D001613:beta 2-Microglobulin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D001613:beta 2-Microglobulin