Development of 3-D poly(trimethylenecarbonate-co-epsilon-caprolactone)-block-poly(p-dioxanone) scaffold for bone regeneration with high porosity using a wet electrospinning method.
The three dimensional (3-D) poly(trimethylenecarbonate-co-epsilon-caprolactone)-block-poly(p-dioxanone) scaffold was made using a wet electrospinning method and its application as a tissue engineered matrix was evaluated for bone regeneration. The scaffold was highly porous (90%) and interconnected among pores. Under scanning electron microscopy, the cells of the center of the scaffold showed healthy well attached shape even at 4 days after seeding. The osteoblastic MC3T3-E1 cells proliferated 1.2 times faster at 4 day, 1.5 times faster at 7 days after seeding as compared with the control in the scaffold (P < 0.05). The activity of alkaline phosphatase, a bone formation marker, of cells seeded in the scaffold was nearly four times faster compared to control 28 days after seeding (P < 0.05). Taken together, newly developed 3-D poly(trimethylenecarbonate-co-epsilon-caprolactone)-block-poly(p-dioxanone) scaffold is a promising candidate for bone regeneration.
Shin
Teo Jeon
TJ
Department of Dental Anesthesiology and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, 110-768, Republic of Korea.
Park
Sook Young
SY
Kim
Hyun Jeong
HJ
Lee
Hak Jun
HJ
Youk
Ji Ho
JH
D000469:Alkaline Phosphatase / Q000032:analysis; D001672:Biocompatible Materials; D001861:Bone Regeneration; D002448:Cell Adhesion; D002455:Cell Division; D004146:Dioxanes; D006801:Humans; D007783:Lactones; D008855:Microscopy, Electron, Scanning; D010011:Osteocytes / Q000201:enzymology / Q000502:physiology / Q000648:ultrastructure; D011091:Polyesters; D011108:Polymers; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D004146:Dioxanes; D007783:Lactones; D011091:Polyesters; D011108:Polymers; C410438:TMC-ECL copolymer; C079733:poly-4-dioxan-2-one; D000469:Alkaline Phosphatase