title: [MEKC-DAD fingerprint of Radix et Rhizoma Rhei].
abstract: OBJECTIVE
  To establish the analytical method for the fingerprint of Radix et Rhizoma Rhei by MEKC-DAD and compare the fingerprints of Radix et Rhizoma Rhei and its processed products.
  
  
  METHODS
  Based on the mode of micellar electrokinetic chromatography, 25 mmol x L(-1) borax -25 mmol x L(-1) SDS-10% acetonitrile was selected for the running buffer (pH 9.2). The separation voltage was 12 kV and the detection wavelength was set at 254 nm. Rhein was used as a reference standard, the chromatographic fingerprint was determined via the data analyzed by fuzzy cluster and fingerprint similarity evaluation software to compare the similarity of samples.
  
  
  RESULTS
  MEKC-DAD fingerprints with 11 common peaks of 10 batches of Radix et Rhizoma Rhei from the place of the genuine were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Radix et Rhizoma Rhei and its processed products, there were obvious differences in the relative areas of common peaks.
  
  
  CONCLUSIONS
  The method is reliable, accurate and can be used for quality control of Radix et Rhizoma Rhei.
authors.affiliation: Pharmacology College, Nanjing University of Chinese Medicine, Nangjing 210046, China. liuxunh1959@sohu.com
authors.forename: Xunhong
authors.initials: X
authors.lastname: Liu
authors.forename: Junsong
authors.initials: J
authors.lastname: Li
authors.forename: Yuechan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
authors.forename: Yin
authors.initials: Y
authors.lastname: Di
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal