Effects of a high-protein diet on host resistance to Paracoccidioides brasiliensis in mice.
We investigated the effects of high protein intake on host resistance to Paracoccidioides brasiliensis. Two-d fasted mice were infected with P. brasiliensis and refed on diets with three different levels (54%, 20%, and 5%) of casein. The mice refed the 54% casein diet showed reduced antifungal activity in the spleen and liver as compared with the mice refed the 5% or the 20% casein diet. After infection, increases in spleen and liver mRNA levels of myeloperoxidase, cathepsin-G, and elastase-2 were more profound in the mice refed the 54% casein diet as compared with the mice refed the 5% or the 20% casein diet. Infected mice refed the 54% casein diet exhibited greater interferon (IFN)-gamma production in the spleen and liver and higher levels of thiobarbituric acid reactive substances (TBARSs) in the liver as compared with those refed the 5% casein diet. These results indicate that high protein intake impairs host resistance to P. brasiliensis.
Oarada
Motoko
M
Medical Mycology Research Center, Chiba University, Chiba, Japan. motoko.o@faculty.chiba-u.jp
Igarashi
Miki
M
Tsuzuki
Tsuyoshi
T
Kamei
Katsuhiko
K
Hirasaka
Katsuya
K
Nikawa
Takeshi
T
Miyazawa
Teruo
T
Nakagawa
Kiyotaka
K
Gonoi
Tohru
T
D000818:Animals; D002364:Caseins / Q000276:immunology; D056649:Cathepsin G / Q000032:analysis; D004044:Dietary Proteins / Q000008:administration & dosage*; D005260:Female; D054884:Host-Pathogen Interactions / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010228:Paracoccidioides / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000276:immunology*; D009195:Peroxidase / Q000032:analysis; D012697:Serine Endopeptidases / Q000032:analysis; D013154:Spleen / Q000201:enzymology / Q000276:immunology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D002364:Caseins; D004044:Dietary Proteins; D017392:Thiobarbituric Acid Reactive Substances; D007371:Interferon-gamma; D009195:Peroxidase; D012697:Serine Endopeptidases; D056649:Cathepsin G; C117309:pancreatic elastase II