Gels containing MMP inhibitors prevent dental erosion in situ.
Matrix metalloproteinase (MMP) inhibition has been shown to reduce dentin caries progression, but its role in dental erosion has not yet been assessed. This study tested the hypothesis that gels containing MMP inhibitors (epigallocatechin gallate-EGCG and chlorhexidine) can prevent dental erosion. Volunteers (n = 10) wore palatal devices containing bovine dentin blocks (n = 10/group) treated for 1 min with EGCG at 10 (EGCG10) or 400 microM (EGCG400), chlorhexidine at 0.012%, F at 1.23% (NaF), and no vehicle (placebo). Erosion was performed with Coca-Cola (5 min) 4X/day during 5 days. The wear, assessed by profilometry (mean +/- SD, microm), was significantly reduced by the gels containing MMP inhibitors (0.05 +/- 0.02(a), 0.04 +/- 0.02(a), and 0.05 +/- 0.02(a) for EGCG10, EGCG400, and chlorhexidine, respectively) when compared with NaF (0.79 +/- 0.35(b)) and placebo gels (1.77 +/- 0.35(b)) (Friedman and Dunn's tests, p < 0.01). The use of gels delivering MMP inhibitors was shown to prevent erosion and opens a new perspective for protection against dental erosion.
Kato
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Bauru Dental School, University of São Paulo, Department of Biological Sciences, Al. Octávio Pinheiro Brisolla, 9-75, Bauru-SP 17012-901, Brazil.
Leite
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Hannas
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Buzalaf
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D000328:Adult; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000627:therapeutic use; D002253:Carbonated Beverages / Q000009:adverse effects; D002327:Cariostatic Agents / Q000008:administration & dosage / Q000627:therapeutic use; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D002417:Cattle; D002710:Chlorhexidine / Q000008:administration & dosage / Q000627:therapeutic use*; D003804:Dentin / Q000187:drug effects / Q000473:pathology; D005782:Gels; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D008855:Microscopy, Electron, Scanning; D010919:Placebos; D011480:Protease Inhibitors / Q000008:administration & dosage / Q000627:therapeutic use*; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000627:therapeutic use; D013997:Time Factors; D014077:Tooth Erosion / Q000473:pathology / Q000517:prevention & control*; D055815:Young Adult
D000891:Anti-Infective Agents, Local; D002327:Cariostatic Agents; D005782:Gels; D061965:Matrix Metalloproteinase Inhibitors; D010919:Placebos; D011480:Protease Inhibitors; D002392:Catechin; D012969:Sodium Fluoride; C045651:epigallocatechin gallate; D002710:Chlorhexidine