title: Nanomagnetic competition assay for low-abundance protein biomarker quantification in unprocessed human sera.
abstract: A novel giant magnetoresistive sensor and uniform high-magnetic-moment FeCo nanoparticles (12.8 nm)-based detecting platform with minimized detecting distance was developed for rapid biomolecule quantification from body fluids. Such a system demonstrates specific, accurate, and quick detection and quantification of interleukin-6, a low-abundance protein and a potential cancer biomarker, directly in 4 muL of unprocessed human sera. This platform is expected to facilitate the identification and validation of disease biomarkers. It may eventually lead to a low-cost personal medical device for chronic disease early detection, diagnosis, and prognosis.
authors.affiliation: Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors.forename: Yuanpeng
authors.initials: Y
authors.lastname: Li
authors.forename: Balasubramanian
authors.initials: B
authors.lastname: Srinivasan
authors.forename: Ying
authors.initials: Y
authors.lastname: Jing
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yao
authors.forename: Marie A
authors.initials: MA
authors.lastname: Hugger
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Wang
authors.forename: Chengguo
authors.initials: C
authors.lastname: Xing
mesh_terms: D015415:Biomarkers / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D003035:Cobalt / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015850:Interleukin-6 / Q000097:blood*; D007501:Iron / Q000737:chemistry; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; D015850:Interleukin-6; D003035:Cobalt; D007501:Iron