title: Distribution of Chlamydia trachomatis ompA genovars and the new variant of C. trachomatis in the Göteborg area, Sweden.
abstract: The aims of this study were to assess the proportion of the new variant of Chlamydia trachomatis (nvCT) and the distribution of ompA genovars among C. trachomatis-positive patients in the Göteborg area, Sweden. Consecutive urine samples positive for C. trachomatis using BD ProbeTec ET (177 patients, 88 men and 89 women) were collected. An nvCT-specific real-time polymerase chain reaction (PCR) assay was used to investigate the nvCT prevalence. To identify the genovars, a 990-bp ompA DNA segment from 105 specimens was sequenced. Seventeen percent (30/177) of all specimens contained nvCT. Nine different genovars were identified. About 50% were of genovar E, followed by F 16%, G 11%, K 8%, and D 5%, representing about 90% of the specimens in Göteborg. The occurrence of nvCT and the dominance of genovar E in Göteborg is similar to those in other areas of Sweden. To cover about 90% of the C. trachomatis infections in Sweden, the serovars D, E, F, G, and K should be included in future vaccines based on the major outer membrane protein.
authors.affiliation: Department of Biomedicine, Göteborg University, The Sahlgrenska Academy, Box 435, 405 30 Göteborg, Sweden. teresa.lagergard@microbio.gu.se
authors.forename: T
authors.initials: T
authors.lastname: Lagergård
authors.forename: R
authors.initials: R
authors.lastname: Hadad
authors.forename: P
authors.initials: P
authors.lastname: Tunbäck
authors.forename: L
authors.initials: L
authors.lastname: Lindholm
authors.forename: G-B
authors.initials: GB
authors.lastname: Löwhagen
authors.forename: M
authors.initials: M
authors.lastname: Unemo
mesh_terms: D000293:Adolescent; D000328:Adult; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D016009:Chi-Square Distribution; D002690:Chlamydia Infections / Q000453:epidemiology* / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D013548:Sweden / Q000453:epidemiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins