title: Solution structure and dynamics of mouse ARMET.
abstract: ARMET is an endoplasmic reticulum (ER) stress-inducible protein that is required for maintaining cell viability under ER stress conditions. However, the exact molecular mechanisms by which ARMET protects cells are unknown. Here, we have analyzed the solution structure of ARMET. ARMET has an entirely alpha-helical structure, which is composed of two distinct domains. Positive charges are dispersed on the surfaces of both domains and across a linker structure. Trypsin digestion and (15)N relaxation experiments indicate that the tumbling of the N-terminal and C-terminal domains is effectively independent. These results suggest that ARMET may hold a negatively charged molecule using the two positively charged domains.
authors.affiliation: Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Hoseki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sasakawa
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Momoe
authors.initials: M
authors.lastname: Maeda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kubota
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nagata
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D012996:Solutions
chemical_list: C534671:MANF protein, mouse; D009414:Nerve Growth Factors; D011506:Proteins; D012996:Solutions