title: Simultaneous cocirculation of both European varicella-zoster virus genotypes (E1 and E2) in Mexico city.
abstract: Full-length genome analysis of varicella-zoster virus (VZV) has shown that viral strains can be classified into seven different genotypes: European (E), Mosaic (M), and Japanese (J), and the E and M genotypes can be further subclassified into E1, E2, and M1 through 4, respectively. The distribution of the main VZV genotypes in Mexico was described earlier, demonstrating the predominance of E genotype, although other genotypes (M1 and M4) were also identified. However, no information regarding the circulation of either E genotype in the country is available. In the present study, we confirm the presence of both E1 and E2 genotypes in the country and explore the possibility of coinfection as the triggering factor for increased virulence among severe cases. A total of 61 different European VZV isolates collected in the Mexico City metropolitan area from 2005 to 2006 were typed by using a PCR method based on genotype-specific primer amplification. Fifty isolates belonged to the E1 genotype, and the eleven remaining samples were classified as E2 genotypes. No coinfection with both E genotypes was identified among these specimens. We provide here new information on the distribution of VZV genotypes circulating in Mexico City.
authors.affiliation: Departamento de Genoma de Patógenos, Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Carpio 470, Mexico City 11340, Mexico.
authors.forename: Araceli
authors.initials: A
authors.lastname: Rodríguez-Castillo
authors.forename: Gilberto
authors.initials: G
authors.lastname: Vaughan
authors.forename: José Ernesto
authors.initials: JE
authors.lastname: Ramírez-González
authors.forename: Alejandro
authors.initials: A
authors.lastname: Escobar-Gutiérrez
mesh_terms: D015897:Comorbidity; D017931:DNA Primers / Q000235:genetics; D016679:Genome, Viral; D005838:Genotype; D006562:Herpes Zoster / Q000453:epidemiology* / Q000473:pathology / Q000821:virology*; D014645:Herpesvirus 3, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D014774:Virulence
chemical_list: D017931:DNA Primers