title: 5-Carboxyfluorescein tagged N-phenylanthranilamide as a tracer reagent for fluorescence polarization: a robust method to screen MAPK pathway allosteric inhibitors.
abstract: Fluorescence polarization using N(alpha)-(fluorescein-5-carbonyl)-N(epsilon)-(N-[2-fluoro-4-iodophenyl]-3,4-difluoroanthraniloyl)lysyl amide is capable of rapidly identifying inhibitor ligands that bind to an allosteric site in MEK1.
authors.affiliation: School of Biomedical Sciences, University of Nottingham Medical School, Queen's Medical Centre, Nottingham, UK NG7 2UH.
authors.forename: Z Nooshin
authors.initials: ZN
authors.lastname: Rezvani
authors.forename: R John
authors.initials: RJ
authors.lastname: Mayer
authors.forename: Weng C
authors.initials: WC
authors.lastname: Chan
mesh_terms: D000494:Allosteric Regulation; D000577:Amides / Q000737:chemistry*; D002460:Cell Line; D005452:Fluoresceins / Q000737:chemistry*; D005454:Fluorescence Polarization; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D020935:MAP Kinase Signaling System; D011485:Protein Binding; D062367:ortho-Aminobenzoates / Q000737:chemistry*
chemical_list: D000577:Amides; D005452:Fluoresceins; D007202:Indicators and Reagents; D062367:ortho-Aminobenzoates; C045132:4-carboxyfluorescein; C001209:fenamic acid