[Determination of plasma protein binding rate of bufalin].
OBJECTIVE
To study plasma protein binding rate of bufalin.


METHODS
HPLC was employed to determine the concentration of bufalin. Plasma protein binding rate studies were conducted by equilibrium dialysis method. The influence of drug concentration and plasma in different species on plasma protein binding rate were studied.


RESULTS
There was no significant difference in the plasma protein binding rates at low, middle and high bufalin concentrations in dilution medium. The protein binding rate of bufalin in human plasma was higher than in the rat plasma.


CONCLUSIONS
Bufalin has higher protein binding extent with both rat plasma and human plasma.
Liu
Ying
Y
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Chen
Zhiqiang
Z
Tao
Beilei
B
Feng
Nianping
N
Zhao
Jihui
J
D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D002018:Bufanolides / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008297:Male; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley
D001798:Blood Proteins; D002018:Bufanolides; C022777:bufalin