[Electrooptical properties of the microbial suspensions during a cell's interaction with the antibodies of a different specificity].
The electrooptical abilities of the microbial suspensions during a cells interaction with antibodies (ABs) of a different specificity have been studied on the example of the Azospirillum brasilense Sp245 cells and their interaction with the polyclonal monospecific and polyspecific antibodies. Measuring of the orientational spectra of the cells has been performed using the ELUS electrooptical analyzer. A discrete frequency set of an orienting electric field (740, 1000, 1450, 2000, and 2800 kHz) was used. It has been shown that an interaction of the polyspecific AB with the investigated cells redoubles the value of an electrooptical signal of the cells' suspension as compared with the monospecific antibodies. These findings can be used for a development a new method of microorganism detection.
Guliĭ
O I
OI
Matora
L Iu
LIu
Burygin
G L
GL
Dykman
L A
LA
Ignatov
V V
VV
Ignatov
O V
OV
D000907:Antibodies, Bacterial / Q000737:chemistry* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D015806:Azospirillum brasilense / Q000276:immunology* / Q000302:isolation & purification; D001419:Bacteria / Q000302:isolation & purification*; D015374:Biosensing Techniques; D004560:Electricity; D055620:Optical Phenomena
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal