Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster.
We have expressed the pqqABCDE gene cluster from Gluconobacter oxydans, which is involved in pyrroloquinoline quinone (PQQ) biosynthesis, in Escherichia coli, resulting in PQQ accumulation in the medium. Since the gene cluster does not include the tldD gene needed for PQQ production, this result suggests that the E. coli tldD gene, which shows high homology to the G. oxydans tldD gene, carries out that function. The synthesis of PQQ activated d-glucose dehydrogenase in E. coli and the growth of the recombinant was improved. In an attempt to increase the production of PQQ, which acts as a vitamin or growth factor, we transformed E. coli with various recombinant plasmids, resulting in the overproduction of the PQQ synthesis enzymes and, consequently, PQQ accumulation--up to 6 mM--in the medium. This yield is 21.5-fold higher than that obtained in previous studies.
Yang
Xue-Peng
XP
School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China. biotech2009@163.com
Zhong
Gui-Fang
GF
Lin
Jin-Ping
JP
Mao
Duo-Bin
DB
Wei
Dong-Zhi
DZ
D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D020600:Gluconobacter oxydans / Q000235:genetics* / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism; D005810:Multigene Family; D045542:PQQ Cofactor / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids
D029968:Escherichia coli Proteins; C098431:TldD protein, E coli; D045542:PQQ Cofactor; D042843:Glucose 1-Dehydrogenase