title: The use of the ccdB lethal gene for constructing a zero background vector in order to clone blunt-end PCR products.
authors.affiliation: Key Lab of Ministry of Health for Hormone and Development, Tianjin Medical University Institute of Endocrinology, Tianjin 300070, China.
authors.forename: Li-Ling
authors.initials: LL
authors.lastname: Hu
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Zhang
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Li
authors.forename: Bao-Li
authors.initials: BL
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005804:Genes, Lethal; D005822:Genetic Vectors / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; C068356:CcdB protein, Plasmid F; D004247:DNA