Detection of vesicant-induced upper airway mucosa damage in the hamster cheek pouch model using optical coherence tomography.
Hamster cheek pouches were exposed to 2-chloroethyl ethyl sulfide [CEES, half-mustard gas (HMG)] at a concentration of 0.4, 2.0, or 5.0 mg/ml for 1 or 5 min. Twenty-four hours post-HMG exposure, tissue damage was assessed by both stereomicrography and optical coherence tomography (OCT). Damage that was not visible on gross visual examination was apparent in the OCT images. Tissue changes were found to be dependent on both HMG concentration and exposure time. The submucosal and muscle layers of the cheek pouch tissue showed the greatest amount of structural alteration. Routine light microscope histology was performed to confirm the OCT observations.
Hammer-Wilson
Marie J
MJ
University of California, Beckman Laser Institute and Medical Clinic, Irvine, California 92612, USA.
Nguyen
Vi
V
Jung
Woong-Gyu
WG
Ahn
Yehchen
Y
Chen
Zhongping
Z
Wilder-Smith
Petra
P
D000818:Animals; D002610:Cheek / Q000293:injuries / Q000473:pathology; D002619:Chemical Warfare Agents / Q000633:toxicity; D006224:Cricetinae; D007509:Irritants / Q000633:toxicity*; D008297:Male; D008647:Mesocricetus; D008853:Microscopy; D009061:Mouth Mucosa / Q000187:drug effects* / Q000293:injuries / Q000473:pathology*; D009151:Mustard Gas / Q000031:analogs & derivatives* / Q000633:toxicity; D041623:Tomography, Optical Coherence / Q000379:methods*
D002619:Chemical Warfare Agents; D007509:Irritants; C031278:2-chloroethyl ethyl sulfide; D009151:Mustard Gas