title: Pressure-driven laminar flow switching for rapid exchange of solution environment around surface adhered biological particles.
abstract: This paper describes a technique for rapidly exchanging the solution environment near a surface by displacing laminar flow fluid streams using sudden changes in applied pressure. The method employs off-chip solenoid valves to induce pressure changes, which is important in keeping the microfluidic design simple and the operation of the system robust. The performance of this technique is characterized using simulation and validated with experiments. This technique adds to the microfluidic tool box that is currently available for manipulating the solution environment around biological particles and molecules.
authors.affiliation: Dept. of Chemistry, University of Washington, Seattle, WA 98195, USA.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Allen
authors.forename: Graham
authors.initials: G
authors.lastname: Milne
authors.forename: Byron R
authors.initials: BR
authors.lastname: Doepker
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Chiu
mesh_terms: D001704:Biopolymers / Q000737:chemistry; D002448:Cell Adhesion; D002469:Cell Separation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010316:Particle Size; D011312:Pressure; D012996:Solutions; D013499:Surface Properties
chemical_list: D001704:Biopolymers; D012996:Solutions