Highly sensitive optical detection of specific protein in breast cancer cells using microstructured fiber in extremely low sample volume.
A simple optical method using hollow-core photonic crystal fiber for protein detection has been described. In this study, estrogen receptor (ER) from a MCF-7 breast carcinoma cell lysates immobilized inside a hollow-core photonic crystal fiber was detected using anti-ER primary antibody with either Alexa Fluor 488 (green fluorescent dye) or 555 (red Fluorescent dye) labeled Goat anti-rabbit IgG as the secondary antibody. The fluorescence fingerprints of the ERalpha protein were observed under fluorescence microscope, and its optical characteristics were analyzed. The ERalpha protein detection by this proposed method is based on immuno binding from sample volume as low as 50 nL. This method is expected to offer great potential as a biosensor for medical diagnostics and therapeutics applications.
Padmanabhan
Saraswathi
S
Nanyang Technological University, School of Mechanical and Aerospace Engineering, Singapore.
Shinoj
Vengalathunadakal K
VK
Murukeshan
Vadakke M
VM
Padmanabhan
Parasuraman
P
D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D047628:Estrogen Receptor alpha / Q000032:analysis* / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D055767:Immobilized Proteins / Q000032:analysis / Q000737:chemistry; D008856:Microscopy, Fluorescence; D055095:Optics and Photonics; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor; D047628:Estrogen Receptor alpha; D005456:Fluorescent Dyes; D055767:Immobilized Proteins