Synthesis of functional proteins within liposomes.
In living cells, biochemical reaction systems are enclosed in the small lipidic compartments. To experimentally simulate various biochemical reactions occurring in extant cells, cell-sized lipid vesicles (liposomes) are used to reconstruct an artificial model cell. We present methods for encapsulation of the protein synthesis system inside liposomes and for measurement of the in liposome synthesis reaction using a fluorescence-activated cell sorter. These techniques would enable us to perform detailed analysis of the biochemical reactions occurring in the microcompartments and have the potential to reveal the role of compartmentalization in cellular systems.
Sunami
Takeshi
T
Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Osaka, Japan.
Matsuura
Tomoaki
T
Suzuki
Hiroaki
H
Yomo
Tetsuya
T
D000818:Animals; D002474:Cell-Free System; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008081:Liposomes; D014176:Protein Biosynthesis; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics
D008081:Liposomes; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D001616:beta-Galactosidase