Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34+38- compartment.
The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34(+)CD38(-) fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34(+)CD38(-) cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34(+)CD38(-) fraction.
Genetica Medica, Dipartimento di Medicina, Chirurgia e Odontoiatria, Università di Milano, Via A di Rudinì 8, 20142 Milan, Italy.
Laura
L
Pedranzini
Federica
F
Mottadelli
Simona
S
Ronzoni
Franca
F
Rossella
Manuela
M
Ferracin
Ivana
I
Magnani
Gaia
G
Roversi
Patrizia
P
Colapietro
Massimo
M
Negrini
Pier Giuseppe
PG
Pelicci
Lidia
L
Larizza
D051997:ADP-ribosyl Cyclase 1 / Q000032:analysis*; D018952:Antigens, CD34 / Q000032:analysis*; D045744:Cell Line, Tumor; D002894:Chromosomes, Human, Pair 4; D055028:Comparative Genomic Hybridization; D020869:Gene Expression Profiling; D006801:Humans; D016130:Immunophenotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000276:immunology; D035683:MicroRNAs / Q000032:analysis*; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics
D018952:Antigens, CD34; D035683:MicroRNAs; D019009:Proto-Oncogene Proteins c-kit; D051997:ADP-ribosyl Cyclase 1