[Determination of ethyl glucuronide in blood by GC-MS/MS].
OBJECTIVE
To develop a method for determining ethyl glucuronide(EtG) in human blood with gas chromatograph-tandem mass spectrometry (GC-MS/MS).


METHODS
Human blood protein was precipitated with acetonitrile. The supernatant was transferred and air flow dried after centrifugated. The residue was derived with N, O-bis (trimethylsilyl)trifluoroacetamide (BSTFA), and analyzed with GC-MS/MS.


RESULTS
The detection limit of EtG in blood was 0.05 microg/mL. Calibration curve covered a span from 0.1-10 microg/mL with a good linear relationship (r = 0.999 9). The method showed a excellent performance when was used to authentic blood sample analysis for EtG.


CONCLUSIONS
The method is suitable for blood EtG analysis.
Shen
Bao-Hua
BH
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China.
Yan
Hui
H
Zhao
Hui
H
Zhuo
Xian-Yi
XY
D000428:Alcohol Drinking / Q000097:blood; D000437:Alcoholism / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D053593:Forensic Toxicology / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005965:Glucuronates / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015415:Biomarkers; D005965:Glucuronates; C093924:ethyl glucuronide