An RNA-mediated silencing pathway utilizes the coordinated synthesis of two distinct populations of siRNA.
In this issue of Molecular Cell, Gent et al. (2010) describe the participation of two siRNA populations, generated by two different RNA-directed RNA polymerases, in a pathway to silence expression of endogenous genes in Caenorhabditis elegans.
Maine
Eleanor M
EM
Department of Biology, Syracuse University, 107 College Place, Syracuse, NY 13244, USA. emmaine@syr.edu
D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D039642:Eukaryotic Initiation Factors / Q000378:metabolism; D009154:Mutation; D010766:Phosphorylation; D034622:RNA Interference; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000096:biosynthesis*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism*; D043244:Ribonuclease III / Q000378:metabolism; D014158:Transcription, Genetic
D029742:Caenorhabditis elegans Proteins; D039642:Eukaryotic Initiation Factors; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012324:RNA-Dependent RNA Polymerase; C472756:RNA-directed RNA polymerase RRF-3, C elegans; D043244:Ribonuclease III