Ultrasensitive detection of non-amplified genomic DNA by nanoparticle-enhanced surface plasmon resonance imaging.
Technologies today available for the DNA detection rely on a combination of labeled probes hybridized to target sequences which are amplified by polymerase chain reaction (PCR). Direct detection methods that eliminate the requirement for both PCR and labeling steps could afford faster, cheaper and simpler devices for the analysis of small amounts of unamplified DNA. In this work we describe the results obtained in the ultrasensitive detection of non-amplified genomic DNA. We analyzed certified reference materials containing different amounts of genetically modified DNA by using a detection method which combines the nanoparticle-enhanced surface plasmon resonance imaging (SPRI) biosensing to the peptide nucleic acids (PNAs) improved selectivity and sensitivity in targeting complementary DNA sequences. The method allowed us to obtain a 41 zM sensitivity in targeting genomic DNA even in the presence of a large excess of non-complementary DNA.
D'Agata
Roberta
R
Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, I-95125 Catania, Italy.
Corradini
Roberto
R
Ferretti
Cristina
C
Zanoli
Laura
L
Gatti
Marcello
M
Marchelli
Rosangela
R
Spoto
Giuseppe
G
D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D004274:DNA, Recombinant / Q000032:analysis / Q000235:genetics; D006046:Gold; D053768:Metal Nanoparticles; D020135:Peptide Nucleic Acids; D030821:Plants, Genetically Modified / Q000235:genetics; D013025:Glycine max / Q000235:genetics; D020349:Surface Plasmon Resonance / Q000379:methods* / Q000706:statistics & numerical data
D018744:DNA, Plant; D004274:DNA, Recombinant; D020135:Peptide Nucleic Acids; D006046:Gold; D004247:DNA