Effect of 3(')-daidzein sulfonic sodium on the anti-oxidation of retinal ischemia/reperfusion injury in rats.
OBJECTIVE
To research the 3(')-daidzein sulfonic sodium's (DSS) effect on anti-oxidation of retinal ischemia/reperfusion (RI/R) injury in rats.


METHODS
RI/R in rats was made by reperfusion for 1 h after occlusion of common carotid artery (CCA) for 1 h and the rats were divided into four groups randomly, including sham control group, model group of RI/R injury, low-dosage DSS group and high-dosage DSS group. 1 ml/kg NS was injected through sublingual vein after CCA was dissociated in sham group. Other groups were treated with normal saline, low-dosage DSS (1 mg/kg) and high-dosage DSS (2 mg/kg) through sublingual vein respectively before occlusion of CCA. After occlusion of CCA for 1 h and reperfusion for 1 h, blood was put out and separated into the serum. Then detect the contents of MDA and NO, the activity of SOD and GSH-PX.


RESULTS
(1) The content of MDA was increased in model group (P<0.05), and low-dosage DSS can reverse it (P<0.05). (2) The content of serum NO in model group was lower than in sham group (P<0.001). The content of serum NO in model group is lower than in low-dosage DSS group (P<0.05) and high-dosage DSS group (P<0.01) respectively. (3) The activity of GSH-PX in high-dosage group was higher than in model group (P<0.05). (4) The activity of SOD in low-dosage group was higher than in model group (P<0.05).


CONCLUSIONS
DSS can decrease the cellular damage and protect the optical function in RI/R injury through elevating the content of NO, the activity of SOD and GSH-PX, and increasing anti-oxidation.
Preclinical Medical College, Gannan Medical University, Ganzhou, Jiangxi Province, China.
Huang
H
Zhihua
Li
L
Liangdong
Li
L
Xiao
Cheng
C
Fang
Zeng
Z
Jing
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000097:blood; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009569:Nitric Oxide / Q000097:blood; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000378:metabolism*; D012160:Retina / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D013482:Superoxide Dismutase / Q000097:blood
C560856:3'-daidzein sulfonic sodium; D000975:Antioxidants; D007529:Isoflavones; D009569:Nitric Oxide; D008315:Malondialdehyde; C004742:daidzein; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase