Culture of K562 human myeloid leukemia cells in presence of fibronectin expresses and secretes MMP-9 in serum-free culture medium.
The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility, gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study the fibronectin-integrin mediated signalling and modulation of MMP expression. Our experimental findings demonstrate that exposure of K562 cells in serum free medium in presence of fibronectin up-regulates the expression of pro-MMP-9 within 2 hrs. Phosphorylation of focal adhesion kinase (FAK), ERK, PI-3K and nuclear translocation of EGFR and NF-kB upon FN binding demonstrate possible involvement of FAK/PI-3K/ERK signalling pathways in the fibronectin-integrin mediated up regulation of MMP-9 expression.
Dutta
Anindita
A
Department of Receptor Biology & Tumor Metastasis, Chittaranjan National Cancer Institute 37, S P Mukherjee Road, Kolkata, India.
Sen
Triparna
T
Chatterjee
Amitava
A
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D016895:Culture Media, Serum-Free; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
D016895:Culture Media, Serum-Free; D005353:Fibronectins; D016023:Integrins; D007797:Laminin; D019715:Tissue Inhibitor of Metalloproteinase-1; D019869:Phosphatidylinositol 3-Kinases; D051416:Focal Adhesion Protein-Tyrosine Kinases; D048049:Extracellular Signal-Regulated MAP Kinases; D020780:Matrix Metalloproteinase 9
FAK; MMP-9; NF-kB; fibronectin; α5β1 integrin