Solubilization of membrane proteins with novel N-acylamino acid detergents.
N-Acylamino acids are a new family of versatile biological surfactants capable of extracting integral membrane proteins of various topologies from the biological membrane, in many instances surpassing the efficiency of commercial detergents.
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 277-8562, Japan.
Ryota
R
Abe
Jose M M
JM
Caaveiro
Motonori
M
Kudou
Kouhei
K
Tsumoto
D018528:ATP-Binding Cassette Transporters / Q000302:isolation & purification; D000596:Amino Acids / Q000737:chemistry; D001426:Bacterial Proteins / Q000302:isolation & purification; D003902:Detergents / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000302:isolation & purification; D025602:Halorhodopsins / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000302:isolation & purification*; D026901:Membrane Transport Proteins / Q000302:isolation & purification; D015394:Molecular Structure; D055550:Protein Stability; D012995:Solubility; D013053:Spectrophotometry; D013501:Surface-Active Agents / Q000737:chemistry; D049490:Systems Biology; D013816:Thermodynamics
D018528:ATP-Binding Cassette Transporters; D000596:Amino Acids; D001426:Bacterial Proteins; D003902:Detergents; D029968:Escherichia coli Proteins; D025602:Halorhodopsins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C079726:MsbA protein, Bacteria; D013501:Surface-Active Agents; C412443:YIDC protein, E coli