Solubilization of membrane proteins with novel N-acylamino acid detergents.
N-Acylamino acids are a new family of versatile biological surfactants capable of extracting integral membrane proteins of various topologies from the biological membrane, in many instances surpassing the efficiency of commercial detergents.
Abe
Ryota
R
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 277-8562, Japan.
Caaveiro
Jose M M
JM
Kudou
Motonori
M
Tsumoto
Kouhei
K
D018528:ATP-Binding Cassette Transporters / Q000302:isolation & purification; D000596:Amino Acids / Q000737:chemistry; D001426:Bacterial Proteins / Q000302:isolation & purification; D003902:Detergents / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000302:isolation & purification; D025602:Halorhodopsins / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000302:isolation & purification*; D026901:Membrane Transport Proteins / Q000302:isolation & purification; D015394:Molecular Structure; D055550:Protein Stability; D012995:Solubility; D013053:Spectrophotometry; D013501:Surface-Active Agents / Q000737:chemistry; D049490:Systems Biology; D013816:Thermodynamics
D018528:ATP-Binding Cassette Transporters; D000596:Amino Acids; D001426:Bacterial Proteins; D003902:Detergents; D029968:Escherichia coli Proteins; D025602:Halorhodopsins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C079726:MsbA protein, Bacteria; D013501:Surface-Active Agents; C412443:YIDC protein, E coli