Layer by layer chitosan/alginate coatings on poly(lactide-co-glycolide) nanoparticles for antifouling protection and Folic acid binding to achieve selective cell targeting.
Polyelectrolyte multilayers (PEMs) composed of two natural polysaccharides-chitosan (Chi) and alginate (Alg) were deposited by Layer by layer (LbL) assembly on top of biocompatible poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs). Folic acid (FA) or FA grafted poly(ethylene glycol) (PEG-FA) were covalently bounded to the PEMs via carbodiimide chemistry. The assembly of biocompatible PEMs was monitored on planar surfaces by means of the quartz crystal microbalance with dissipation (QCM-D) technique and on top of PLGA NPs by means of ζ-potential measurements. BSA was used as model protein to characterize protein adsorption on PEMs. QCM-D showed protein deposition could not be observed on the Chi/Alg multilayer, for both Chitosan and Alginate as top layers. Finally, cellular uptake experiments were carried out by co-culture of HepG2 cells in presence of NPs. Flow Cytometry and confocal laser scanning microscopy (CLSM) were used to investigate the influence of the surface chemistry of the NPs on uptake. For the HepG2 cell line significantly less uptake of PLGA NPs coated with Chi/Alg than the bare NPs was observed but the uptake increased after attachment of FA molecules.
Zhou
Jie
J
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Romero
Gabriela
G
Rojas
Elena
E
Ma
Lie
L
Moya
Sergio
S
Gao
Changyou
C
D000464:Alginates / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D016503:Drug Delivery Systems; D005492:Folic Acid / Q000737:chemistry* / Q000494:pharmacology; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D056945:Hep G2 Cells; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011098:Polyglactin 910 / Q000737:chemistry* / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000494:pharmacology; D014803:Vitamin B Complex / Q000737:chemistry / Q000494:pharmacology
D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D014803:Vitamin B Complex; D012710:Serum Albumin, Bovine; D011098:Polyglactin 910; D020723:Glucuronic Acid; D048271:Chitosan; D005492:Folic Acid