Evaluation of photo-induced cellular damage using photoautotrophic cultures of puk-bung hairy roots as a sensing tool.
In photoautotrophic cultures of pak-bung hairy roots, strong light irradiation (22 W/m(2)) increased the content of reactive oxygen species (ROS) in the cells, resulting in chlorophyll (Chl) degradation and DNA injury. The Chl degradation rate, R(D), can be used as a parameter to measure the cellular damage caused by photo-induced stress. The presence of the antioxidants ascorbic acid, chlorogenic acid or catechin reduced the R(D), while lowering the content of ROS and moderating the DNA injury.
Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Motomu
M
Nishioka
Yoshihiro
Y
Ojima
Yuki
Y
Tsushima
Masahiro
M
Matsumoto
Masahito
M
Taya
D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D001709:Biotechnology; D002392:Catechin / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000494:pharmacology; D002734:Chlorophyll / Q000378:metabolism; D004249:DNA Damage; D018744:DNA, Plant / Q000378:metabolism / Q000528:radiation effects; D027741:Ipomoea / Q000378:metabolism* / Q000528:radiation effects*; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D018462:Photobiology; D018517:Plant Roots / Q000378:metabolism / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D046509:Tissue Culture Techniques
D000975:Antioxidants; D018744:DNA, Plant; D017382:Reactive Oxygen Species; D002734:Chlorophyll; D002726:Chlorogenic Acid; D002392:Catechin; D001205:Ascorbic Acid