Bryothamnion seaforthii lectin relaxes vascular smooth muscle: involvement of endothelium and NO synthase.
Bryothamnion seaforthii lectin (BSL) induced reversible concentration-related relaxation of endothelized aorta, maximal at 30 microg/ml (IC(50)= 4.8 +/- 0.6 microg/ml). This effect was inhibited by L-NAME and reversed by mucin, probably via interaction with a specific lectin-binding site on the endothelium activating nitric oxide synthase.
Instituto Superior de Ciências Biomédicas, CCS, Universidade Estadual do Ceará, Av. Paranjana 1700, 60740-000, Fortaleza-CE, Brasil.
Ricardo F
RF
Lima
David N
DN
Criddle
Pedro Marcos G
PM
Soares
Samile Pereira
SP
Ribeiro
Benildo S
BS
Cavada
Kyria S
KS
Nascimento
Alexandre H
AH
Sampaio
Ana Maria S
AM
Assreuy
D020418:Algal Proteins / Q000737:chemistry*; D000704:Analysis of Variance; D000818:Animals; D001011:Aorta / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000201:enzymology; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D009200:Myocardial Contraction / Q000187:drug effects; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000461:Rhodophyta / Q000737:chemistry*
D020418:Algal Proteins; D037102:Lectins; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester