Effects of streptozotocin-induced long-term diabetes on parietal cell function and morphology in rats.
Gastric pathology is a common complication in diabetes mellitus. The aim of the study was to evaluate the functions and morphological changes of the parietal cells of the rat stomach after streptozotocin-induced diabetes. Diabetes mellitus was induced in Wistar rats by a single intraperitoneal injection of streptozotocin (60 mg/kg body weight). The rats were weighed weekly and sacrificed after 6 months. The glandular portion of the stomach was removed and processed for H(+)-K(+)-ATPase immunohistochemistry and light and electron microscopy studies. Acid secretion was measured in vivo. After 6 months of diabetes, the mean weight of the rats was significantly lower (P < 0.001) compared to control. The mean weight of the stomach to body weight percentage increased significantly (P < 0.001) compared to control. The blood glucose level in diabetic rats was significantly higher (P < 0.001) than in normal control. Diabetic rats showed significant (P < 0.001) decrease in basal and stimulated acid secretion when compared to control. Electron micrographs of the parietal cells of glandular stomach of diabetic rats revealed significant (P < 0.0002) reduction in the number of mitochondria and a small though not significant increase in the number of canaliculi in the parietal cells compared with normal. Immunohistochemistry showed reduced H(+)-K(+)-ATPase (P < 0.00001) compared to control. Long-term diabetes induces morphological as well as functional changes in gastric parietal cells. The decrease in the number of mitochondria accompanied by reduced in H(+)-K(+)-ATPase in parietal cells may explain the reduced acid secretion observed in diabetics.
Department of Pharmacology, Faculty of Medicine and Health Sciences, UAE University, PO Box 17666 Al Ain, UAE. sbastaki@uaeu.ac.ae
Salim M A
SM
Bastaki
Ernest
E
Adeghate
Irwin S
IS
Chandranath
Naheed
N
Amir
Saeed
S
Tariq
Rashed S
RS
Hameed
Abdu
A
Adem
D000818:Animals; D001835:Body Weight; D048909:Diabetes Complications / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D005744:Gastric Acid / Q000378:metabolism; D017506:H(+)-K(+)-Exchanging ATPase / Q000032:analysis; D008928:Mitochondria; D009929:Organ Size; D010295:Parietal Cells, Gastric / Q000473:pathology* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach; D013311:Streptozocin; D013997:Time Factors
D013311:Streptozocin; D017506:H(+)-K(+)-Exchanging ATPase