Characterization of SVEP1, KIAA, and SRPX2 in an in vitro cell culture model of endotoxemia.
To assess the influence of unknown factors in endotoxemia, a conditioned medium, achieved by the stimulation of THP1 monocytes with lipopolysaccharide (LPS) [4h], was used for the stimulation of human umbilical vein endothelial cells (HUVECs) [16h]. SVEP1, KIAA0247, and SRPX2 were selected after microarray analysis. To study their possible functions, siRNAs of SVEP1, KIAA0247, or SRPX2 were used for the transfection of HUVECs and cells were stimulated with conditioned medium [16h]. Inhibition of SVEP1 expression resulted in an increase of soluble intercellular adhesion molecule (sICAM) 1 (10%) and soluble E-selectin (sE-selectin) (19%). Inhibition of SRPX2 led to an increase of sICAM (11%) and sE-selectin (14%). KIAA0247 negative HUVECs showed a decrease in monocyte chemoattractant protein (MCP) 1 of 16%. SVEP1 and SRPX2 seemed to act as regulators of ICAM1 and E-selectin shedding and influence the expression of membrane bound adhesion molecules.
Center for Biomedical Technology, Department for Clinical Medicine and Biotechnology, Danube University Krems, Austria. schwanzer-pfeiffer@donau-uni.ac.at
Dagmar
D
Schwanzer-Pfeiffer
Eva
E
Rossmanith
Anita
A
Schildberger
Dieter
D
Falkenhagen
D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D003165:Complement System Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D019446:Endotoxemia / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D006801:Humans; D007249:Inflammation; D019041:L-Selectin / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins; D046228:Microarray Analysis; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics
D015815:Cell Adhesion Molecules; D017077:Culture Media, Conditioned; D016207:Cytokines; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; C116881:SRPX2 protein, human; C551654:SUSD6 protein, human; C508516:SVEP1 protein, human; D019041:L-Selectin; D003165:Complement System Proteins