Production of human growth hormone by Lactococcus lactis.
A synthetic gene coding for human growth hormone was expressed in Lactococcus lactis. The presence of the recombinant protein was assayed and quantified using ELISA tests. Human growth hormone was detected at high concentrations and displayed a biological activity similar to the one shown by commercial human growth hormone.
Margolles
Abelardo
A
Departamento de Microbiología y Bioquímica de Productos Lácteos, Instituto de Productos Lácteos de Asturias (CSIC), Carretera de Infiesto s/n 3300 Villaviciosa, Asturias, Spain. amargolles@ipla.csic.es
Moreno
José Antonio
JA
Ruiz
Lorena
L
Marelli
Belkis
B
Magni
Christian
C
de Los Reyes-Gavilán
Clara G
CG
Ruas-Madiedo
Patricia
P
D001483:Base Sequence; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D017931:DNA Primers / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D019382:Human Growth Hormone / Q000096:biosynthesis* / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology
D017931:DNA Primers; D011994:Recombinant Proteins; D019382:Human Growth Hormone