In vivo wide-area cellular imaging by side-view endomicroscopy.
In vivo imaging of small animals offers several possibilities for studying normal and disease biology, but visualizing organs with single-cell resolution is challenging. We describe rotational side-view confocal endomicroscopy, which enables cellular imaging of gastrointestinal and respiratory tracts in mice and may be extensible to imaging organ parenchyma such as cerebral cortex. We monitored cell infiltration, vascular changes and tumor progression during inflammation and tumorigenesis in colon over several months.
Wellman Center for Photomedicine, Department of Dermatology, Harvard Medical School and Massachusetts General Hospital, Boston, MA, USA.
Pilhan
P
Kim
Euiheon
E
Chung
Hiroshi
H
Yamashita
Kenneth E
KE
Hung
Atsushi
A
Mizoguchi
Raju
R
Kucherlapati
Dai
D
Fukumura
Rakesh K
RK
Jain
Seok H
SH
Yun
D000818:Animals; D003092:Colitis / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology; D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods*; D004195:Disease Models, Animal; D016099:Endoscopy, Gastrointestinal / Q000379:methods*; D018928:Immunity, Mucosal; D007413:Intestinal Mucosa / Q000098:blood supply / Q000276:immunology; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*