The DNA sequence-dependence of nucleosome positioning in vivo and in vitro.
The contribution of histone-DNA interactions to nucleosome positioning in vivo is currently a matter of debate. We argue here that certain nucleosome positions, often in promoter regions, in yeast may be, at least in part, specified by the DNA sequence. In contrast other positions may be poorly specified. Positioning thus has both statistical and DNA-determined components. We further argue that the relative affinity of the octamer for different DNA sequences can vary and therefore the interaction of histones with the DNA is a 'tunable' property.
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK. aat@mrc-lmb.cam.ac.uk
Andrew
A
Travers
Edwige
E
Hiriart
Mark
M
Churcher
Micaela
M
Caserta
Ernesto
E
Di Mauro
D000818:Animals; D001483:Base Sequence; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D006657:Histones / Q000378:metabolism; D006801:Humans; D009707:Nucleosomes / Q000235:genetics* / Q000378:metabolism*
D006657:Histones; D009707:Nucleosomes