Mycobacterium tuberculosis complex differentiation by genomic deletion patterns with multiplex polymerase chain reaction and melting analysis.
Differentiation of Mycobacterium tuberculosis complex (MTC) species is important for patient management. We developed a genomic deletion assay based on multiplex polymerase chain reaction with melting temperature analysis that correctly identified 124 (96%) of 129 MTC isolates. This assay is a fast single-tube method to differentiate members of MTC.
Institute for Clinical and Experimental Pathology, Associated Regional and University Pathologists, Inc., Salt Lake City, UT 84108, USA. afjip@uaa.alaska.edu
June I
JI
Pounder
Clint M
CM
Anderson
Karl V
KV
Voelkerding
Max
M
Salfinger
Jillian
J
Dormandy
Akos
A
Somoskovi
Leonid
L
Heifets
Jenny Jo
JJ
Graham
Douglas R
DR
Storts
Cathy A
CA
Petti
D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017384:Sequence Deletion; D044366:Transition Temperature; D014376:Tuberculosis / Q000382:microbiology*
D004269:DNA, Bacterial