C-type natriuretic peptide: Structural studies, fragment synthesis, and preliminary biological evaluation in human osteosarcoma cell lines.
Natriuretic peptides (NP) are a family of structurally related but genetically distinct hormones/paracrine factors that regulate blood volume, blood pressure, ventricular hypertrophy, pulmonary hypertension, fat metabolism, and long bone growth. In this work we present computational structural analysis of the three human NP in solution, the synthesis and preliminary biological assays of a short fragment of CNP, I(14)GSM(17), together with one small mimetic, GGSM. Synthetic peptides IGSM, GGSM, and full length CNP were preliminary tested for their ability to influence cell growth of three human osteosarcoma cell lines. Synthetic peptides were shown to successfully mimic the biological activity of the full length natural peptide: their effects, although different upon the cell types used, are in accordance with the current literature, designating a possible role for CNP, and its derivatives, in skeletogenesis.
Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.za della Scienza 2 20126 Milano, Italy.
Nasrin
N
Shaikh
Laura
L
Russo
Elena
E
Papaleo
Paolo
P
Giannoni
Luca
L
De Gioia
Francesco
F
Nicotra
Rodolfo
R
Quarto
Laura
L
Cipolla
D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D003198:Computer Simulation; D006801:Humans; D008969:Molecular Sequence Data; D020098:Natriuretic Peptide, C-Type / Q000138:chemical synthesis* / Q000737:chemistry; D012516:Osteosarcoma / Q000473:pathology; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry; D016415:Sequence Alignment
D010446:Peptide Fragments; D020098:Natriuretic Peptide, C-Type