Characterization of a new defensin from cowpea (Vigna unguiculata (L.) Walp.).
Using Phaseoleae defensins available in databases, a putative defensin gene was isolated in cowpea (Vigna unguiculata (L.) Walp.) and cloned from genomic cowpea DNA. The putative mature defensin sequence displays the characteristic defensins residues arrangement, secondary and tertiary structures were predicted and splicing analysis was performed. Using RT-PCR, defensin expression and differences in response to biotic stimuli between infected and non infected plants were tested.
Padovan
Lara
L
Department of Life Sciences, University of Trieste - Via Giorgieri, 1 - 34127 Trieste, Italy. padovan@burlo.trieste.it
Segat
Ludovica
L
Tossi
Alessandro
A
Calsa
Tercilio
T
Ederson
Akio Kido
AK
Brandao
Lucas
L
Guimarães
Rafael L
RL
Pandolfi
Valesca
V
Pestana-Calsa
Maria Clara
MC
Belarmino
Luís Carlos
LC
Benko-Iseppon
Ana Maria
AM
Crovella
Sergio
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D017798:Comovirus; D023082:Defensins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein / Q000379:methods*
D023082:Defensins; D010940:Plant Proteins