Chromosome wide analysis of CUGBP1 binding sites identifies the tetraspanin CD9 mRNA as a target for CUGBP1-mediated down-regulation.
CUGBP1 is an RNA-binding protein controlling alternative splicing, mRNA translation and stability. In this work we used a motif scoring approach to identify putative CUGBP1 binding sites for genes located on the human chromosome 12. This allowed us to identify the gene CD9 as a presumptive target for CUGBP1-mediated regulation. In a number of cancers, the tetraspanin CD9 is down-regulated, an event correlated with a bad prognostic. Using a combination of biochemical approaches and CUGBP1 knockdown, we showed that CUGBP1 directly controls CD9 expression.
CNRS, UMR 6061 Institut de Génétique et Développement de Rennes, Expression Génétique et Développement, Université de Rennes I, IFR 140 GFAS, 2 avenue du Professeur Léon Bernard, CS 34317, 35043 Rennes Cedex, France.
Olivier
O
Le Tonquèze
Bernhard
B
Gschloessl
Allen
A
Namanda-Vanderbeken
Vincent
V
Legagneux
Luc
L
Paillard
Yann
Y
Audic
D020413:3' Untranslated Regions; D015703:Antigens, CD / Q000235:genetics*; D001665:Binding Sites; D000067879:CELF1 Protein; D002478:Cells, Cultured; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D015536:Down-Regulation; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA / Q000379:methods; D060245:Tetraspanin 29
D020413:3' Untranslated Regions; D015703:Antigens, CD; C554869:CD9 protein, human; D000067879:CELF1 Protein; C102843:CELF1 protein, human; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D060245:Tetraspanin 29