Strategies for enhanced annotation of a microarray probe set.
We aim to determine the biological relevance of genes identified through microarray-mediated transcriptional profiling of Xenopus sensory organs and brain. Difficulties with genetic data analysis arise because of limitations in probe set annotation and the lack of a universal gene nomenclature. To overcome these impediments, we used sequence based and semantic linking methods in combination with computational approaches to augment probe set annotation on a commercially available microarray. Our curation efforts enabled linkage of probe sets and expression data to public databases, increased the biological significance of our microarray data, and assisted with the tentative identification of unidentified probe sets.
Powers
Tushun R
TR
Department of Biology, New Mexico State University, Las Cruces, NM 88003, USA. tupowers@nmsu.edu
Virk
Selene M
SM
Serrano
Elba E
EE
D019295:Computational Biology / Q000379:methods*; D016208:Databases, Factual; D030541:Databases, Genetic; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020543:Proteome / Q000378:metabolism
D020543:Proteome