Proteomic analysis of Trichoderma atroviride mycelia stressed by organophosphate pesticide dichlorvos.
The proteomic approach is a powerful tool to study microbial response to environmental stress. To evaluate the responses of Trichoderma spp. to the organophosphate pesticide dichlorvos, mycelia of Trichoderma atroviride T23 were exposed to dichlorvos at concentrations of 0, 100, 300, 500, and 1000 microg/mL, respectively. Changes in protein expression were investigated using two-dimensional sodium dodecyl sulfate - polyacrylamide gel electrophoresis. Sixteen protein spots were differentially expressed. They were identified by MALDI-TOF/TOF MS and were found to be linked to energy metabolism, transport, signal transduction, and stress tolerance. Among stress-related proteins, glutathione peroxidase-like protein (GPX), 1,4-benzoquinone reductase, and HEX1 were upregulated by and cyclophilin A induced by 1000 microg/mL dichlorvos when compared with the control. These proteins were considered to be associated with fungal adaptation to adverse conditions. The results will help us to understand molecular mechanisms through which Trichoderma responds to organophosphate pesticides.
Tang
Jun
J
Department of Resources and Environmental Sciences, Shanghai Jiaotong University, P.R. China.
Liu
Lixing
L
Huang
Xiuli
X
Li
Yingying
Y
Chen
Yunpeng
Y
Chen
Jie
J
D004006:Dichlorvos / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000096:biosynthesis*; D025282:Mycelium / Q000187:drug effects* / Q000235:genetics; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological; D014242:Trichoderma / Q000187:drug effects* / Q000235:genetics
D005656:Fungal Proteins; D020543:Proteome; D004006:Dichlorvos