Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione.
Adding a potent antioxidant, glutathione (GSH), to a cryoprotective solution consisting of dimethyl sulfoxide and ethylene glycol and/or postthaw culture medium significantly improved the postthaw viability of mouse embryonic stem cells. This effect, which was caused by a decrease in reactive oxygen species, was only induced by exposure of embryonic stem cells during cryopreservation.
Kim
Gil Ah
GA
Stem Cell and Bioevalution, Major in Biomodulation, WCU, and Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
Lee
Seung Tae
ST
Ahn
Ji Yeon
JY
Park
Jong Heum
JH
Lim
Jeong Mook
JM
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D005615:Freezing; D005978:Glutathione / Q000494:pharmacology*; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism
D003451:Cryoprotective Agents; D017382:Reactive Oxygen Species; D005978:Glutathione