Tobacco mutants with reduced microtubule dynamics are less susceptible to TMV.
A panel of seven SR1 tobacco mutants (ATER1 to ATER7) derived via T-DNA activation tagging and screening for resistance to a microtubule assembly inhibitor, ethyl phenyl carbamate, were used to study the role of microtubules during infection and spread of tobacco mosaic virus (TMV). In one of these lines, ATER2, alpha-tubulin is shifted from the tyrosinylated into the detyrosinated form, and the microtubule plus-end marker GFP-EB1 moves significantly slower when expressed in the background of the ATER2 mutant as compared with the SR1 wild type. The efficiency of cell-to-cell movement of TMV encoding GFP-tagged movement protein (MP-GFP) is reduced in ATER2 accompanied by a reduced association of MP-GFP with plasmodesmata. This mutant is also more tolerant to viral infection as compared with the SR1 wild type, implying that reduced microtubule dynamics confer a comparative advantage in face of TMV infection.
Botanical Institute 1, University of Karlsruhe, Kaiserstrasse 2, D-76128 Karlsruhe, Germany.
Maurice O
MO
Ouko
Adrian
A
Sambade
Katrin
K
Brandner
Annette
A
Niehl
Eduardo
E
Peña
Abdul
A
Ahad
Manfred
M
Heinlein
Peter
P
Nick
D004269:DNA, Bacterial / Q000235:genetics; D008870:Microtubules / Q000378:metabolism*; D009154:Mutation; D048448:Phenylcarbamates / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics / Q000821:virology; D053830:Plant Viral Movement Proteins / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000502:physiology*; D014404:Tubulin / Q000378:metabolism*; D014520:Urethane / Q000494:pharmacology; D014779:Virus Replication
D004269:DNA, Bacterial; D048448:Phenylcarbamates; D053830:Plant Viral Movement Proteins; C036483:T-DNA; D014404:Tubulin; D014520:Urethane; C008866:phenylurethane