GnRH receptor and peritoneal plasmin activity.
Most surgical procedures performed by obstetrician-gynecologists are associated with pelvic adhesions that cause subsequent serious sequelae, including small bowel obstruction, infertility, chronic pelvic pain, and difficulty in postoperative treatment, including complexity during subsequent surgical procedures. This study was conducted to determine if gonadotropin-releasing hormone analogues (GnRHa) affect the expressing tissue-type plasminogen activator (t-PA) and its inhibitor-1 (PAI-1) in peritoneal cells in culture. Human peritoneal Met5A cells were used to examine the effects of GnRHa leuprolide, buserelin and goserelin on the levels of t-PA and PA-1. Antigen concentrations were measured in conditioned media and cell lysates by real-time PCR and ELISA. GnRH receptor (GnRHR) mRNA was determined by RT-PCR. GnRHR mRNA was detected in Met5A cells. Exposure of Met5A cells to GnRHa induced a rapid decrease of PAI-1 level in cultured medium but not in cell lysate (protein and mRNA). These effects of GnRHa on PAI-1 were not associated with any changes in t-PA level. These results suggest that GnRHa may be an effective stimulator of local peritoneal fibrinolytic activity, as it decreases PAI-1 secretion in peritoneal Met5A cells by a mechanism linked to GnRHR.
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Gifu, Japan.
Noriko
N
Suzuki
Akio
A
Yamamoto
Tatsuro
T
Furui
Atsushi
A
Imai
D002064:Buserelin / Q000494:pharmacology; D002478:Cells, Cultured; D004789:Enzyme Activation; D005341:Fibrinolysin / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000494:pharmacology; D017273:Goserelin / Q000494:pharmacology; D006801:Humans; D016729:Leuprolide / Q000494:pharmacology; D010537:Peritoneum / Q000201:enzymology / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics / Q000378:metabolism; D011966:Receptors, LHRH / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010959:Tissue Plasminogen Activator / Q000235:genetics / Q000378:metabolism
D017395:Plasminogen Activator Inhibitor 1; D011966:Receptors, LHRH; C051321:SERPINE1 protein, human; D017273:Goserelin; D007987:Gonadotropin-Releasing Hormone; C083781:LHRH, Ala(6)-Gly(10)-ethylamide-; D010959:Tissue Plasminogen Activator; D005341:Fibrinolysin; D016729:Leuprolide; D002064:Buserelin