[The distribution and expression pro-files of Aspartyl/Asparaginyl beta-hydroxylase (ASPH) in some tumorous cell lines and tissues].
OBJECTIVE
To investigate the distribution and expression profiles of Aspartyl/Asparaginyl beta-hydroxylase (ASPH) in some tumorous cell lines and some tissues. To evaluate the distribution and expression profile of Aspartyl/Asparaginyl beta-hydroxylase, ASPH in some tumor cell lines and tumor tissues.


METHODS
The expression of ASPH in tumor cell lines on transcriptional level transcriptical level was tested by using RT-PCR, and on translational level was tested by cellular immunofluorescence and Western blot. The histologic sections from tumorous and normal tissue were tested immunohistochemistry.


RESULTS
The expression of ASPH could be detected at both the transcriptional level and the translational level in the tumorous cell lines and it distributed in the cell membrane and the cytoplasmic domain.


CONCLUSIONS
ASPH can be wildly used as a potential prognostic marker in neoplasms detection.
Faculty of Life Sciences, Northwestern Polytech-nical University, Xi'an, China. songkaifrank@yahoo.cn
Kai
K
Song
Xiao-ping
XP
Xue
Wei
W
Wang
Ting
T
Huyan
Hua
H
Wang
Hui
H
Yang
Qiong
Q
Xie
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000235:genetics; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000032:analysis* / Q000235:genetics; D009124:Muscle Proteins / Q000032:analysis* / Q000235:genetics; D009369:Neoplasms / Q000737:chemistry / Q000175:diagnosis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D006899:Mixed Function Oxygenases; C419389:ASPH protein, human