Characterization of antibiotic resistance in Vibrio spp. isolated from farmed marine shrimps (Penaeus monodon).
A total of 83 Vibrio isolates from farmed marine shrimps (Penaeus monodon) were tested for the presence of class 1, 2 and 3 integrons, SXT constin and tetracycline resistance-encoding genes. Mutations in the quinolone resistance-determining regions (QRDRs) of the gyrA and parC genes were determined in fluoroquinolone-resistant Vibrio strains (n=17). Five isolates were found to carry class 1 integrons, of which only one contained the partial rumA gene in the variable region. All the Vibrio strains were devoid of class 2 and 3 integrons. Seven isolates harbored SXT constin. None of the Vibrio isolates were positive to the tet(K), tet(L), tet(M), tet(O) and tet(S) genes. Ten fluoroquinolone-resistant Vibrio strains carried a point mutation G-248-T in the gyrA QRDR, leading to a Ser-83-Ile substitution in GyrA, but none of these strains had mutations in the QRDR of the parC gene.
Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Sirikorn
S
Kitiyodom
Sirintip
S
Khemtong
Janenuj
J
Wongtavatchai
Rungtip
R
Chuanchuen
D000818:Animals; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D032023:Integrons; D008826:Microbial Sensitivity Tests; D009154:Mutation; D033561:Penaeidae / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000187:drug effects* / Q000235:genetics*
D004269:DNA, Bacterial; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase