Interactions between sub-10-nm iron and cerium oxide nanoparticles and 3T3 fibroblasts: the role of the coating and aggregation state.
Recent nanotoxicity studies revealed that the physico-chemical characteristics of engineered nanomaterials play an important role in the interactions with living cells. Here, we report on the toxicity and uptake of cerium and iron oxide sub-10-nm nanoparticles by NIH/3T3 mouse fibroblasts. Coating strategies include low-molecular weight ligands (citric acid) and polymers (poly(acrylic acid), M(W) = 2000 g mol(-1)). Electrostatically adsorbed on the surfaces, the organic moieties provide a negatively charged coating in physiological conditions. We find that most particles were biocompatible, as exposed cells remained 100% viable relative to controls. Only the bare and the citrate-coated nanoceria exhibit a slight decrease in mitochondrial activity at very high cerium concentrations (>1 g l(-1)). We also observe that the citrate-coated particles are internalized/adsorbed by the cells in large amounts, typically 250 pg/cell after 24 h incubation for iron oxide. In contrast, the polymer-coated particles are taken up at much lower rates (<30 pg/cell). The strong uptake shown by the citrated particles is related to the destabilization of the dispersions in the cell culture medium and their sedimentation down to the cell membranes. In conclusion, we show that the uptake of nanomaterials by living cells depends on the coating of the particles and on its ability to preserve the colloidal nature of the dispersions.
Matière et Systèmes Complexes, UMR 7057 CNRS, Université Denis Diderot Paris VII, Bâtiment Condorcet, 10 rue Alice Domon et Léonie Duquet, Paris, France.
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M
Safi
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H
Sarrouj
O
O
Sandre
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N
Mignet
J-F
JF
Berret
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002498:Centrifugation; D002563:Cerium / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D008970:Molecular Weight; D041681:NIH 3T3 Cells; D010316:Particle Size; D012542:Scattering, Radiation; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D020099:Coated Materials, Biocompatible; D003470:Culture Media; D005290:Ferric Compounds; C000499:ferric oxide; D002563:Cerium; C030583:ceric oxide