SnO2@Poly(HEMA-co-St-co-VPBA) core-shell nanoparticles designed for selectively enriching glycopeptides followed by MALDI-MS analysis.
The core-shell boronic-acid functionalized nanoparticles SnO(2)@Poly(HEMA-co-St-co-VPBA) are designed for selectively enriching glycopeptides, followed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. Such 60 nm sized core-shell nanoparticles are prepared by means of copolymerization between 2-hydroxyethyl methacrylate (HEMA) grafted on SnO(2) nanoparticles, styrene, and 4-vinylphenylboronic acid (VPBA). All of the synthesis procedures are completed within 3 h. Cyclic boronate esters form between boronic-acid groups on the polymer chains and cis-diol groups on glycopeptides, and thus almost all intact glycopeptides from low-abundant horseradish peroxidase (HRP) and bovine asialofetuin (ASF) are enriched with high selectivity and efficiency. After enrichment, both intact N- and O-glycopeptides are characterized by multistage MS. Furthermore, we successfully apply this method to the human serum sample for characterizing the target glycoproteins haptoglobin and alpha-1-acid-glycoprotein. The present selective enriching method followed by multistage-MS analysis is proven to be a good choice for routine glycopeptide characterization.
Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433 China.
Wen-Wen
WW
Shen
Cheng-Ning
CN
Ma
Su-Feng
SF
Wang
Huan-Ming
HM
Xiong
Hao-Jie
HJ
Lu
Peng-Yuan
PY
Yang
D000595:Amino Acid Sequence; D001212:Asialoglycoproteins / Q000737:chemistry; D001896:Boron Compounds / Q000737:chemistry*; D001897:Boronic Acids / Q000737:chemistry; D060748:Fetuins; D006020:Glycopeptides / Q000032:analysis / Q000737:chemistry*; D006031:Glycosylation; D006242:Haptoglobins / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D011073:Polyamines / Q000737:chemistry; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives* / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017971:Tin Compounds / Q000737:chemistry; D014753:Vinyl Compounds / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000737:chemistry
C427523:4-vinylphenyl boronic acid; D001212:Asialoglycoproteins; D001896:Boron Compounds; D001897:Boronic Acids; D060748:Fetuins; D006020:Glycopeptides; D006242:Haptoglobins; D011073:Polyamines; D011145:Polyvinyls; D017971:Tin Compounds; D014753:Vinyl Compounds; D000509:alpha-Fetoproteins; C017029:asialofetuin; C056971:poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer; D011102:Polyhydroxyethyl Methacrylate; C045358:stannic oxide