Production of human growth hormone by Lactococcus lactis.
A synthetic gene coding for human growth hormone was expressed in Lactococcus lactis. The presence of the recombinant protein was assayed and quantified using ELISA tests. Human growth hormone was detected at high concentrations and displayed a biological activity similar to the one shown by commercial human growth hormone.
Departamento de Microbiología y Bioquímica de Productos Lácteos, Instituto de Productos Lácteos de Asturias (CSIC), Carretera de Infiesto s/n 3300 Villaviciosa, Asturias, Spain. amargolles@ipla.csic.es
Abelardo
A
Margolles
José Antonio
JA
Moreno
Lorena
L
Ruiz
Belkis
B
Marelli
Christian
C
Magni
Clara G
CG
de Los Reyes-Gavilán
Patricia
P
Ruas-Madiedo
D001483:Base Sequence; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D017931:DNA Primers / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D019382:Human Growth Hormone / Q000096:biosynthesis* / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology
D017931:DNA Primers; D011994:Recombinant Proteins; D019382:Human Growth Hormone