A new reliable fluorescence in situ hybridization method for identifying multiple specific cytogenetic abnormalities in acute myeloid leukemia.
The usefulness of the new Chromoprobe Multiprobe AML Panel was evaluated in 80 patients with acute myeloid leukemia (AML) in parallel with conventional cytogenetics. We observed a high concordance using both methods, but the panel was very useful in the detection of an inv(16)(p13q22), a cryptic t(15;17)(q22;q21), and a cryptic deletion of the CBFbeta allele not detected with cytogenetics. Moreover, in six of nine patients (67%) without metaphases or with non-evaluable chromosomes, the panel identified three MLL rearrangements, two monosomy 7, one of them also with del(5q), and one inv(16)(p13q22). Our results indicate that the multiprobe panel can be used as a complementary technique for detection of the most important chromosomal abnormalities in AML using small quantities of sample in only one hybridization experiment. It is also capable of reallocating cases without metaphases or with non-evaluable chromosomes in the appropriate cytogenetic risk group.
Department of Hematology, Hospital Universitario La Fe, 46009 Valencia, Spain.
Ana
A
Valencia
Jose
J
Cervera
Esperanza
E
Such
Mariam
M
Ibañez
Eva
E
Barragán
Oscar
O
Fuster
Pascual
P
Bolufer
Federico
F
Moscardó
Miguel A
MA
Sanz
D002869:Chromosome Aberrations; D007446:Chromosome Inversion; D002884:Chromosomes, Human, Pair 15; D002885:Chromosomes, Human, Pair 16; D002897:Chromosomes, Human, Pair 7; D004252:DNA Mutational Analysis / Q000379:methods; D005260:Female; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008954:Models, Biological; D009006:Monosomy; D051788:Myeloid-Lymphoid Leukemia Protein / Q000235:genetics; D015514:Oncogene Proteins, Fusion / Q000032:analysis / Q000235:genetics; D015203:Reproducibility of Results; D013379:Substrate Specificity / Q000235:genetics
C497422:KMT2A protein, human; D015514:Oncogene Proteins, Fusion; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase