A novel, selective, and rapid fluorimetric method for the simultaneous analysis of coproporphyrin and uroporphyrin in urine.
A simple and rapid spectrofluorimetric method is described for the determination of the closely overlapping mixture of coproporphyrin (CP) and uroporphyrin (UP) in urine samples. Matrix Isopotential Synchronous Fluorescence Spectrometry (MISFS) was applied to improve the spectral resolution for the severely overlapped spectra of the urinary porphyrins. First-order derivative technique eliminates the background interference of each component on the other. Using these two techniques together, selectivity was improved, while maintaining a high sensitivity, and time-consuming separation processes and multiple scanning processes were avoided. The limits of detection were 0.15 nmol L(-1) and 0.1 nmol L(-1) for CP and UP, respectively. The concentrations of CP and UP were determined from the peak amplitudes of the Derivative Matrix Isopotential Synchronous Fluorescence (DMISF) spectra, at their detection points where the interference was suppressed. Porphyrins excretion in urine samples, collected from normal subjects, was studied. A comparison between the new method and the anion-exchange chromatographic method of Martinez and Mills was established using Bland-Altman method and the results indicate that these two methods are in a good agreement with each other.
Department of Chemistry and Key Laboratory of Analytical Sciences of the Xiamen University, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Ali Abbas Falih
AA
Shindi
Pei-Chen
PC
Zhou
Zhe-Xiang
ZX
Zou
Yao-Qun
YQ
Li
D002852:Chromatography, Ion Exchange; D003306:Coproporphyrins / Q000652:urine*; D006801:Humans; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014578:Uroporphyrins / Q000652:urine*
D003306:Coproporphyrins; D014578:Uroporphyrins