Synthesis of phosphatidylinositols having various inositol stereoisomers by engineered phospholipase D.
Phospholipase D-mediated synthesis of phosphatidylinositols having various inositol stereoisomers was studied. Seven inositol stereoisomers were tested, all of which were found to be substrates of the enzyme, generating the corresponding phosphatidylinositols. Based on the substrate specificity, models for the recognition of inositol by the enzyme were proposed.
Laboratory of Molecular Biotechnology, Department of Bioengineering Sciences, Graduate School of Bioagricultural Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Akari
A
Ozaki
Atsushi
A
Masayama
Hideo
H
Nakano
Yugo
Y
Iwasaki
D019943:Amino Acid Substitution; D020134:Catalytic Domain / Q000235:genetics; D008958:Models, Molecular; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D010716:Phosphatidylinositols / Q000096:biosynthesis* / Q000737:chemistry*; D010739:Phospholipase D / Q000235:genetics* / Q000378:metabolism*; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013237:Stereoisomerism; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics; D013379:Substrate Specificity
D010716:Phosphatidylinositols; D011994:Recombinant Proteins; D010739:Phospholipase D