Effect of a calcium glycerophosphate fluoride dentifrice formulation on enamel demineralization in situ.
OBJECTIVE
To evaluate in situ the effect and mechanisms involved in the anticariogenic effect of a calcium glycerophosphate fluoride dentifrice.


METHODS
In a double-blind, crossover design, a non-F dentifrice (negative control), a F dentifrice and a F dentifrice containing 0.13% CaGP were compared regarding the inhibition of enamel demineralization. Both F dentifrices contained 1500 microg F/g (w/w) as sodium monofluorophosphate (MFP). Bovine enamel blocks were mounted in contact with a S. mutans test plaque, in palatal appliances worn by 10 volunteers. 30 minutes after treatment with the dentifrices, a sucrose rinse was performed and enamel demineralization was assessed after an additional 45 minutes.


RESULTS
No significant difference was observed among groups in the calcium and inorganic phosphate concentrations in the fluid phase of the test plaque 30 minutes after the dentifrice use (P > 0.05), but F concentration was significantly higher for both F dentifrices (P < 0.05). Also, the dentifrices did not differ regarding the pH before or 5 minutes after the sugar challenge (P > 0.05). A higher mineral loss was observed for the non-F dentifrice group (P < 0.05), but no significant difference was observed between the F dentifrices containing CaGP or not (P > 0.05). Using this in situ model, the findings suggested that CaGP at the concentration tested did not enhance the inhibition of enamel demineralization promoted by F dentifrice.
Piracicaba Dental School, University of Campinas - UNICAMP, P.O. Box 52, 13414-903 Piracicaba, SP, Brazil. litenuta@fop.unicamp.br
Livia Maria Andaló
LM
Tenuta
Maximiliano Sergio
MS
Cenci
Altair A Del Bel
AA
Cury
Tatiana
T
Pereira-Cenci
Cínthia Pereira Machado
CP
Tabchoury
Gisele Pedroso
GP
Moi
Jaime Aparecido
JA
Cury
D000818:Animals; D002118:Calcium / Q000032:analysis; D002326:Cariogenic Agents / Q000494:pharmacology; D002327:Cariostatic Agents / Q000627:therapeutic use*; D002417:Cattle; D018592:Cross-Over Studies; D003743:Dental Enamel / Q000187:drug effects* / Q000473:pathology; D003773:Dental Plaque / Q000737:chemistry / Q000382:microbiology; D004311:Double-Blind Method; D005260:Female; D005459:Fluorides / Q000032:analysis / Q000627:therapeutic use*; D005994:Glycerophosphates / Q000627:therapeutic use*; D006244:Hardness; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D010710:Phosphates / Q000627:therapeutic use; D010758:Phosphorus / Q000032:analysis; D013295:Streptococcus mutans / Q000187:drug effects; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors; D017001:Tooth Demineralization / Q000378:metabolism / Q000517:prevention & control*
D002326:Cariogenic Agents; D002327:Cariostatic Agents; D005994:Glycerophosphates; D010710:Phosphates; C012980:fluorophosphate; D010758:Phosphorus; D013395:Sucrose; D005459:Fluorides; D002118:Calcium