Detection of Caprine herpesvirus 1-specific antibodies in goat sera using an enzyme-linked immunosorbent assay and serum neutralization test.
An enzyme-linked immunosorbent assay (ELISA) was developed to detect immunoglobulin G (IgG) antibodies directed to whole Caprine herpesvirus 1 (CpHV-1). Sera from 248 goats were obtained from CpHV-1-free and CpHV-1-infected flocks and were subjected to both IgG ELISA and serum neutralization (SN) assays, with the latter considered the gold standard for the diagnosis of CpHV-1 infection. In flocks where CpHV-1 infection was detected, 57 sera were negative by the SN and the ELISA tests and 97 sera were positive with both tests. Thus, although based on biologically different principles, the ELISA was as sensitive as the SN assay in detecting seropositive animals and could be efficiently used as a faster and less expensive alternative to the SN test for the screening of many samples.
Marinaro
Mariarosaria
M
Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena, 299 00161 Rome, Italy. mary_marinaro@libero.it.
Bellacicco
Anna Lucia
AL
Tarsitano
Elvira
E
Camero
Michele
M
Colao
Valeriana
V
Tempesta
Maria
M
Buonavoglia
Canio
C
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005189:False Positive Reactions; D015511:Goat Diseases / Q000097:blood* / Q000276:immunology; D006041:Goats; D006566:Herpesviridae Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D009500:Neutralization Tests / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D018140:Varicellovirus / Q000276:immunology*