Adenanthera pavonina trypsin inhibitor retard growth of Anagasta kuehniella (Lepidoptera: Pyralidae).
Anagasta kuehniella is a polyphagous pest that feeds on a wide variety of stored products. The possible roles suggested for seed proteinase inhibitors include the function as a part of the plant defensive system against pest via inhibition of their proteolytic enzymes. In this study, a trypsin inhibitor (ApTI) was purified from Adenanthera pavonina seed and was tested for insect growth regulatory effect. The chronic ingestion of ApTI did result in a significant reduction in larval survival and weight. Larval and pupal developmental time of larvae fed on ApTI diet at 1% was significantly longer; the larval period was extended by 5 days and pupal period was 10 days longer, therefore delaying by up to 20 days and resulting in a prolonged period of development from larva to adult. As a result, the ApTI diet emergence rate was only 28% while the emergence rate of control larvae was 80%. The percentage of surviving adults (%S) decreased to 62%. The fourth instar larvae reared on a diet containing 1% ApTI showed a decrease in tryptic activity of gut and that no novel proteolytic form resistant to ApTI was induced. In addition, the tryptic activity in ApTI -fed larvae was sensitive to ApTI. These results suggest that ApTI have a potential antimetabolic effect when ingested by A. kuehniella.
Laboratório de Purificação de Proteínas e suas Funções Biológicas, Depto. De Tecnologia de Alimentos e Saúde Pública, CCBS, Universidade Federal de Mato Grosso do Sul, CP 549, CEP 79070-900, Brazil. bioplant@terra.com.br
Maria Lígia Rodrigues
ML
Macedo
Roberta Aparecida
RA
Durigan
Desiree Soares
DS
da Silva
Sérgio
S
Marangoni
Maria das Graças Machado
Md
Freire
José Roberto Postali
JR
Parra
D000818:Animals; D004435:Eating / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D007915:Lepidoptera / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D010572:Pest Control, Biological / Q000379:methods; D012639:Seeds / Q000737:chemistry; D014361:Trypsin Inhibitors / Q000494:pharmacology*
D014361:Trypsin Inhibitors