Wound healing effects of gingival fibroblasts cultured in animal-free medium.
OBJECTIVE
The purpose of this study was to develop a graft material made of gingival fibroblasts cultured in animal-free medium (HFDM1).


METHODS
We examined the effects of human serum (HS) on cell growth and wound healing capability, demonstrated by cytokine production, of gingival fibroblasts cultured in HFDM1. Subsequently, the capability of fibroblasts cultured in HFDM1 with 2% HS to promote the healing of skin defects was evaluated using nude mice.


RESULTS
The proliferation of human gingival fibroblasts was increased when HS at a concentration of 0.5-2% was added to HFDM1. Wound healing cytokines, including transforming growth factor-beta, keratinocyte growth factor, hepatocyte growth factor, vascular endothelial growth factor, and IL-6 produced by gingival fibroblasts were increased by adding 2% HS to HFDM1. In addition, gingival fibroblasts cultured in HFDM1 with 2% HS improved wound healing of mouse skin defects as well as those cultured in Dulbecco's modified Eagle's medium with 10% fetal calf serum.


CONCLUSIONS
Gingival fibroblasts cultured in HFDM1 with 2% HS may be useful as a graft material for reconstruction.
Department of Oral and Maxillofacial Surgery, Division of Cervico-Gnathostmatology, Programs for Applied Biomedicine, Graduate School of Biomedical Sciences, Hiroshima University, Minami-Ku, Hiroshima, Japan. hiyoko@hiroshima-u.ac.jp
H
H
Nishi
K
K
Ohta
M
M
Takechi
S
S
Yoneda
M
M
Hiraoka
N
N
Kamata
D000818:Animals; D001769:Blood; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D003470:Culture Media; D016207:Cytokines / Q000032:analysis; D051523:Fibroblast Growth Factor 7 / Q000032:analysis; D005347:Fibroblasts / Q000502:physiology* / Q000637:transplantation; D005881:Gingiva / Q000166:cytology / Q000502:physiology* / Q000637:transplantation; D017228:Hepatocyte Growth Factor / Q000032:analysis; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012871:Skin Diseases / Q000601:surgery; D023822:Tissue Engineering / Q000379:methods; D016212:Transforming Growth Factor beta / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis; D014945:Wound Healing / Q000502:physiology
D003470:Culture Media; D016207:Cytokines; D015850:Interleukin-6; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D051523:Fibroblast Growth Factor 7; D017228:Hepatocyte Growth Factor