Isolation and identification of poisonous triterpenoids from Elaeodendron croceum.
A phytochemical investigation of the poisonous Elaeodendron croceum leaves guided by cytotoxicity against Vero cells led to the isolation of five known compounds: 20-hydroxy-20-epi-tingenone (1), tingenone (2), tingenine B (3), 11alpha-hydroxy-beta-amyrin (4) and naringenin (5). Compounds 1 and 2 showed the highest toxicity against Vero cells (IC(50) values 2.65 nM and 8.23 microM, respectively). Cytotoxicity of the isolated compounds against three human cancer cell lines, HeLa, MCF-7 and SNO was also determined. Compounds 1 and 2 again showed the highest cytotoxicity, with IC(50) values ranging between 2.47 and 0.43 microM. This is the first report on the isolation of poisonous compounds from E. croceum, a species well known for its toxicity.
Department of Plant Science, University of Pretoria, Pretoria 0002, South Africa.
Thembela
T
Yelani
Ahmed A
AA
Hussein
J J Marion
JJ
Meyer
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D029753:Celastraceae / Q000737:chemistry*; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D006801:Humans; D015394:Molecular Structure; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D014709:Vero Cells
D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes