Reliability of automated volumetric measurement of multiple growing follicles in controlled ovarian hyperstimulation.
OBJECTIVE
To evaluate the reliability of a computer-assisted approach for automatically measuring ovarian follicles during controlled ovarian hyperstimulation (COH).


METHODS
Prospective, comparative study.


METHODS
Hospital Béclère, Clamart, France.


METHODS
Twenty-seven infertile IVF-ET candidates undergoing COH.


METHODS
Just before the oocyte retrieval, growing follicles (n=72) had their mean diameters measured and their volumes determined semimanually by virtual organ computer-aided analysis (VOCAL) and automatically by SonoAVC. Follicles were sorted in small (12-16 mm; n=35) and large (>16 mm; n=37) growing follicles. Measures were compared with the follicular fluid volume.


METHODS
Concordance of results using intraclass correlation coefficient and limits of agreement methods, respectively.


RESULTS
Overall, VOCAL (median: 3.42 mL; range: 0.98-9.68 mL) and SonoAVC (3.25 mL; 0.98-8.63 mL) measurements were equivalent to the corresponding actual follicle volume (3.20 mL; 0.80-10.20 mL). The intraclass correlation coefficient values between follicular fluid volume and mean diameter, VOCAL, and SonoAVC were 0.51, 0.95, and 0.98, respectively, for small follicles, and 0.80, 0.93, and 0.92, respectively, for large follicles. 95% limits of agreement between actual volume and VOCAL (-1.09 to +1.07 mL) and SonoAVC (-1.08 to +0.84 mL) measurements were comparable in both groups.


CONCLUSIONS
Automated measurement of multiple follicular volumes using SonoAVC is a simple technique, which reliability is superior to usual diameter measurements and comparable to VOCAL. This technologic refinement invites us to switch toward volumetric monitoring of follicle growth during COH.
AP-HP, Service de Gynécologie-Obstétrique et Médecine de la Reproduction, Hôpital Antoine Béclère, University Paris-Sud, Clamart and INSERM, Clamart, France.
Frederic
F
Lamazou
Elisangela
E
Arbo
Samuel
S
Salama
Michaël
M
Grynberg
René
R
Frydman
Renato
R
Fanchin
D000328:Adult; D005260:Female; D015571:Follicular Fluid / Q000166:cytology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007247:Infertility, Female / Q000000981:diagnostic imaging; D054315:Oocyte Retrieval / Q000295:instrumentation / Q000379:methods; D009929:Organ Size; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000187:drug effects / Q000254:growth & development*; D010062:Ovulation Induction / Q000379:methods; D010363:Pattern Recognition, Automated; D015203:Reproducibility of Results; D014463:Ultrasonography