Protoplast transformation of filamentous fungi.
The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.
Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY, USA.
B Gillian
BG
Turgeon
Bradford
B
Condon
Jinyuan
J
Liu
Ning
N
Zhang
D004262:DNA Restriction Enzymes / Q000494:pharmacology*; D018274:Electroporation / Q000379:methods*; D005658:Fungi / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D016681:Genome, Fungal; D016133:Polymerase Chain Reaction / Q000379:methods*; D011523:Protoplasts / Q000378:metabolism*; D014170:Transformation, Genetic
D004262:DNA Restriction Enzymes