Macrophage chemotactic protein-1 mRNA levels in non-Hodgkin lymphoma.
Non-Hodgkin lymphoma (NHL) is one of the most common malignancies whose incidence increases, and the treatment results are not satisfactory. The aim of this study was to determine the capacity of NHL to produce MCP-1, chemokine that induces chemotaxis of macrophages and lymphoid cells. The mRNA expression and protein MCP-1 expression were determined in the samples of 20 patients with NHL and 8 reactive tonsils. MCP-1 mRNA was detected in 8/8 tonsils and in 19/20 patients with NHL by real-time PCR analysis. In addition, the amount of detected MCP-1 cDNA was significantly higher in patients with limited stage, good IPI, normal level of fibrinogen and LDH. Finally, in patients with aggressive NHL, the level of MCP-1 cDNA was higher than in indolent tumours. Immunohistochemical analysis revealed that majority of stromal elements such as macrophages, endothelial and smooth muscle cells in reactive as well as in neoplastic lymphoid tissue showed strong cytoplasmic MCP-1 expression. Moderate cytoplasmic MCP-1 expression was also observed in reactive lymphocytes, while tumour cells of indolent NHL were mostly pale in comparison with aggressive lymphomas which predominantly demonstrated intense MCP-1 staining. These intriguing preliminary results emphasize the need for further investigations that must be conducted on the representative sample with concordant measurement of serum MCP-1 level.
Department of Haematology, Clinical Hospital Centre Rijeka, Rijeka, Croatia.
Toni
T
Valković
Antica
A
Duletić-Načinović
Sanja
S
Stifter
Milena
M
Hasan
Ita
I
Hadžisejdić
David
D
Zombori
Blaženka
B
Grahovac
Nives
N
Jonjić
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D003593:Cytoplasm / Q000737:chemistry; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000737:chemistry; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology*; D008264:Macrophages / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D014066:Palatine Tonsil / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D012333:RNA, Messenger