Effect of an injectable 3D scaffold for osteoblast differentiation depends on bead size.
The objective of this study was to evaluate the effect of beta-tricalcium phosphate (beta-TCP) bead size on the behavior of KUSA/A1 mouse osteoblasts when the beta-TCP beads are used as the solid phase of a scaffold in which alginate was used as the gel phase. KUSA/A1 cells were loaded onto a three-dimensional (3D) scaffold fabricated from beta-TCP beads with diameters ranging from 300 to 500 microm (small beads), 500-700 microm (medium beads) and 700-850 microm (large beads); cells were cultured for 3, 7 and 14 days. Scanning electron microscope observations showed that each bead was connected in a network consisting of the alginate gel and KUSA/A1 cellular matrix that was tightly bonded to form a 3D structure. After 3 days, cells in the 3D scaffold with medium beads had a significantly higher alkaline phosphatase activity (ALP) than cells in the other scaffolds. However, by 7 and 14 days in culture there was no significant difference in DNA levels, ALP activity or osteocalcin expression. At 8 weeks, only the composite containing small beads and KUSA/A1 cells had turned completely into bone in vivo. Thus, bead size may influence the success of bone formation in this context.
Department of Biomaterials, Osaka Dental University, Osaka, Hirakata, Osaka, Japan. yoshiya@cc.osaka-dent.ac.jp
Yoshiya
Y
Hashimoto
Seita
S
Adachi
Tomonori
T
Matsuno
Kazuhiko
K
Omata
Yamauchi
Y
Yoshitaka
Yasuyuki
Y
Ozeki
Yoshikazu
Y
Umezu
Tazuko
T
Satoh
Masaaki
M
Nakamura
D000818:Animals; D018786:Bone Substitutes / Q000008:administration & dosage / Q000737:chemistry*; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000737:chemistry*; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D048091:Guided Tissue Regeneration / Q000295:instrumentation* / Q000379:methods; D007267:Injections; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008819:Mice, Nude; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D054457:Tissue Scaffolds
D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate