Light-activated gene expression from site-specific caged DNA with a biotinylated photolabile protection group.
A new method for site-specific caging of plasmid DNA was developed to enable light-activation of gene expression in living cells by exposure to a low dose of light.
Yamaguchi
Satoshi
S
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, 113-8656, Tokyo, Japan.
Chen
Yanjie
Y
Nakajima
Soh
S
Furuta
Toshiaki
T
Nagamune
Teruyuki
T
D001710:Biotin / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D055668:Photochemical Processes; D010957:Plasmids; D014466:Ultraviolet Rays
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D001710:Biotin; D004247:DNA