[Recovery of movement after spinal cord injury in DO11.10 transgenic mouse].
OBJECTIVE
To analysis the role of T lymphocytes in spinal cord regeneration by comparing the recovery of movement and the morphological changes of injury area between BALB/c and DO11.10 transgenic mice.


METHODS
Producing a crush injury model of spinal cord with special forceps. Analyze the changes of spinal cord injury area with H&E and GFAP, CD11b and lymphocytic immunohistochemical staining. Evaluate the recovery of movement function with Basso-Beattie-Bresnahan (BBB) locomotion testing system at 0, 7, 14 and 21 day post-injury (dpi).


RESULTS
There were thicker and fastened glial scar at 21 dpi in the BALB/c mice but not in DO11.10 mice. The number of macrophages/microglia infiltrated in spinal cord injury area were more in DO11.10 than that in BALB/c mice at 14 dpi. The numbers of T lymphocytes infiltrated in spinal cord injury area were less in DO11.10 than that in BALB/c mice at 21 dpi. In addition, compare to BALB/c mice, the locomotion movement recovery of DO11.10 mice were much more significant within 3 weeks after spinal cord injury by BBB scoring system.


CONCLUSIONS
The infiltrated autoimmune activation T lymphocytes which specifically react to neural antigens are not beneficial to recovery of movement after spinal cord injury in mice.
Wang
Hui
H
Department of Neurobiology, School of Basic Medicine, Xi'an, China.
Guo
Jun
J
Zhao
Yu
Y
Bian
Gan-lan
GL
Liu
Fang-fang
FF
Yu
Cai-yong
CY
Feng
Rui
R
Ju
Gong
G
Wang
Jian
J
D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D017254:Leukemic Infiltration; D008124:Locomotion; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D013119:Spinal Cord Injuries / Q000276:immunology / Q000503:physiopathology*; D013601:T-Lymphocytes / Q000276:immunology