Identification of pathogenic rare yeast species in clinical samples: comparison between phenotypical and molecular methods.
Species identification using both phenotypic and molecular methods and antifungal susceptibility tests was carried out with 60 uncommon clinical yeasts. Our data show that phenotypic methods were insufficient for correct identification (only 25%) and that most of the wrongly identified strains showed a resistant antifungal profile.
Ctra Majadahonda-Pozuelo Km 2, Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain.
Emilio
E
Cendejas-Bueno
Alicia
A
Gomez-Lopez
Emilia
E
Mellado
Juan L
JL
Rodriguez-Tudela
Manuel
M
Cuenca-Estrella
D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods; D009172:Mycology / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology*; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*