Involvement of the IL-6/STAT3/Sca-1 system in proliferation of duct cells following duct ligation in the submandibular gland of mice.
Ligation of the main excretory duct (MED) of the mouse submandibular gland (SMG) induced the expression of Sca-1, a stem cell marker. Sca-1 expression increased prominently in almost all of cells in the duct system, except the acinar cells. Sca-1 induction was accompanied with phosphorylated-STAT3 (Y705) elevation, which was localized in the nuclei of all duct cells. Electrophoretic mobility shift assay (EMSA) confirmed the specific binding of STAT3 to the GAS sequence, a biding site of gamma interferon activating site. Present study suggested one of the initial steps of the tissue regeneration after injury includes STAT3 pathway.
Department of Molecular Oral Physiology, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.
Nunuk
N
Purwanti
Ahmad
A
Azlina
Mileva R
MR
Karabasil
Takahiro
T
Hasegawa
Chenjuan
C
Yao
Tetsuya
T
Akamatsu
Kazuo
K
Hosoi
D000818:Animals; D000950:Antigens, Ly / Q000502:physiology*; D049109:Cell Proliferation; D007249:Inflammation / Q000209:etiology / Q000503:physiopathology; D015850:Interleukin-6 / Q000502:physiology*; D008026:Ligation / Q000009:adverse effects; D008297:Male; D008565:Membrane Proteins / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012038:Regeneration / Q000502:physiology; D050796:STAT3 Transcription Factor / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D013363:Submandibular Gland / Q000166:cytology / Q000503:physiopathology*
D000950:Antigens, Ly; D015850:Interleukin-6; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse