Molecular and clinical features of chronic lymphocytic leukemia with stereotyped B-cell receptors in a Ukrainian cohort.
A fraction of chronic lymphocytic leukemia (CLL) carries highly homologous B-cell receptors, characterized by non-random combinations of immunoglobulin heavy-chain variable (IGHV) genes and heavy-chain complementarity-determining region-3 (HCDR3), often associated with a restricted selection of IG(K/L)V light chains. We analyzed the features of CLL expressing homologous HCDR3 in a cohort of 264 Ukrainian patients by merging them with a recently published reference series of 1426 cases. This approach allowed us to identify 96/264 (36%) cases as expressing homologous HCDR3, subdivided into 47 subsets. Among these, 27 apparently novel subsets were identified, although most of them were composed of two sequences per subset ('potential subsets'). CLL cases belonging to several stereotyped subsets showed HCDR3 homologies with various autoreactive clones. Our analysis identified molecular and clinical features of a Ukrainian cohort of patients with CLL.
Research Center for Radiation Medicine, Academy of Medical Sciences, Kiev, 04053, Ukraine. nbilous@yahoo.com
Nadiia
N
Bilous
Riccardo
R
Bomben
Michele
M
Dal Bo
Daniela
D
Capello
Francesco
F
Forconi
Luca
L
Laurenti
Francesco
F
Bertoni
Dimitar G
DG
Efremov
Roberto
R
Marasca
Giovanni
G
Del Poeta
Zoya
Z
Martina
Iryna
I
Kryachouk
Iryna
I
Dyagil
Gianluca
G
Gaidano
Anatoliy
A
Chumak
Valter
V
Gattei
Iryna
I
Abramenko
D015331:Cohort Studies; D022801:Complementarity Determining Regions / Q000235:genetics; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics; D050438:Genes, Immunoglobulin Heavy Chain / Q000235:genetics; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology*; D011947:Receptors, Antigen, B-Cell / Q000235:genetics*; D027041:Somatic Hypermutation, Immunoglobulin / Q000235:genetics; D015996:Survival Rate; D016896:Treatment Outcome
D022801:Complementarity Determining Regions; D007135:Immunoglobulin Variable Region; D011947:Receptors, Antigen, B-Cell