Conformational change of flagellin for polymorphic supercoiling of the flagellar filament.
The bacterial flagellar filament is a helical propeller rotated by the flagellar motor for bacterial locomotion. The filament is a supercoiled assembly of a single protein, flagellin, and is formed by 11 protofilaments. For bacterial taxis, the reversal of motor rotation switches the supercoil between left- and right-handed, both of which arise from combinations of two distinct conformations and packing interactions of the L-type and R-type protofilaments. Here we report an atomic model of the L-type straight filament by electron cryomicroscopy and helical image analysis. Comparison with the R-type structure shows interesting features: an orientation change of the outer core domains (D1) against the inner core domains (D0) showing almost invariant orientation and packing, a conformational switching within domain D1, and the conformational flexibility of domains D0 and D1 with their spoke-like connection for tight molecular packing.
Maki-Yonekura
Saori
S
Dynamic NanoMachine Project, International Cooperative Research Project, Japan Science and Technology Agency, Suita, Osaka, Japan.
Yonekura
Koji
K
Namba
Keiichi
K
D018407:Bacterial Physiological Phenomena; D005407:Flagella / Q000737:chemistry*; D005408:Flagellin / Q000737:chemistry*; D011487:Protein Conformation
D005408:Flagellin