A rapid assay for detecting antibody against Bluetongue virus with a latex agglutination test using recombinant VP7 antigen.
A latex agglutination test (LAT) for detecting antibody against Bluetongue virus (BTV) in ruminants was developed using latex beads coupled with recombinant VP7 protein. Compared with competitive enzyme-linked immunosorbent assay (ELISA), the specificity and sensitivity of the LAT were 99.0% and 93.0%, respectively. There was excellent agreement between the results obtained by competitive ELISA and the LAT (kappa = 0.930). Because it is rapid and easy to use, the LAT could be used for BTV antibody detection, especially for screening many serum samples.
No. 2049, Heping Road, Shenzhen, Guangdong, P. R. China 518001. ciqhua@yahoo.com.cn
Junxing
J
Yang
Qunyi
Q
Hua
Huanchun
H
Chen
Jianqiang
J
Lv
Bing
B
Chen
Zhouxi
Z
Ruan
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001819:Bluetongue / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D001820:Bluetongue virus / Q000276:immunology*; D007841:Latex Fixation Tests / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012756:Sheep; D014758:Viral Core Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D014758:Viral Core Proteins; C066862:VP7 protein, orbivirus