Regeneration, in vitro glycoalkaloids production and evaluation of bioactivity of callus methanolic extract of Solanum tuberosum L.
Callus and differentiated shoots initiated from Solanum tuberosum L. on MS media containing BA, IAA, and Kin. Glycoalkaloids are produced in callus and shoots in concentrations higher than original tubers using HPLC. Callus methanolic extract had promising anticancer activity with low IC(50) values against human carcinoma cell lines of breast, lymphoplastic leukemia, larynx, liver, cervix, colon, and brain, IC(50) (microg/mL) were 2.7, 3.7, 6, 6.7, 10, 13.6, and 22.3 respectively. Antioxidant capacity of the extract (76.4%) performed using ESR. Preliminary screening showed that the extract exhibited in vitro virucidal activity against Herpes simplex. The extract possessed in vitro schistomicidal and fasciolicidal activity.
Drugs and Pharmaceutical Industries Division, National Research Center, Cairo, Dokki, Egypt. hanan_alashaal@yahoo.com
Hanan Abd Al-Hay
HA
Al-Ashaal
D000470:Alkaloids / Q000096:biosynthesis*; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis; D000975:Antioxidants / Q000032:analysis; D000977:Antiparasitic Agents / Q000032:analysis; D000998:Antiviral Agents / Q000032:analysis; D046508:Culture Techniques; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry*; D018520:Plant Shoots / Q000378:metabolism; D012038:Regeneration; D011198:Solanum tuberosum / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D000977:Antiparasitic Agents; D000998:Antiviral Agents; D010936:Plant Extracts