Molecular replacement studies on crystal structure of DesB1-B2 Despentapeptide (B26-B30) insulin.
Using the crystal structure of Despentapeptide (B26-B30) insulin (DPI as the search model, the crystal structure of DesB1-B2 Despentapeptide (B26-B30) insulin (DesB1-2 DPI) has been studied by the molecular replacement method. There is one DesB1-2 DPI molecule in each crystallographic asymmetric unit. The cross rotation function search and the translation function search show apparent peaks and thus determine the orientation and position of DesB1-2 DPI molecule in the cell respectively. The subsequent three-dimensional structural rebuilding and refinement of DesB1-2 DPI molecule confirm the results by molecular replacement method.
Diao
J
J
State Key Laboratory of Biornacrornolecules, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China.
Wan
Z
Z
Chang
W
W
Liang
D
D