Characterization and applications of serum-free induced adhesion in Jurkat suspension cells.
In this study, we demonstrate that the presence of serum in different media plays an important role in inducing transient and reversible adhesion in Jurkat suspension cells. Attachment of Jurkat cells in two distinct media formulations (serum-fortified and serum-free) to untreated polystyrene (PS), plasma-treated PS, and fibronectin-coated PS was compared. Additional analysis characterized the occurrence of this transient cell adhesion, including attachment rate, reversibility of attachment, and viability and preservation of phenotype in cells during and after attachment. As a demonstration of the utility of this technique, a few applications of transiently adhering Jurkat cells are shown which would be otherwise difficult with freely suspended cells, such as increased gene delivery, confocal-based apoptosis detection, and real-time electric-field effect monitoring in Jurkat cells.
Department of Biological and Agricultural Engineering, Louisiana State University and LSU Agricultural Center, Baton Rouge, Louisiana 70803, USA.
Julianne F
JF
Audiffred
Sarah E
SE
De Leo
Paige K
PK
Brown
Hollie
H
Hale-Donze
W Todd
WT
Monroe
D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002470:Cell Survival; D003470:Culture Media / Q000737:chemistry; D016895:Culture Media, Serum-Free; D006801:Humans; D019169:Jurkat Cells; D011137:Polystyrenes
D003470:Culture Media; D016895:Culture Media, Serum-Free; D011137:Polystyrenes