Prevalence of different Malassezia species in pityriasis versicolor in central India.
BACKGROUND
In the last 10 years, different studies have shown interesting geographical variations in the prevalence of different Malassezia species in pityriasis versicolor.


OBJECTIVE
Identification of Malassezia species isolated from patients with pityriasis versicolor.


METHODS
In 100 patients with pityriasis versicolor, Malassezia species were identified by culture in Sabouraud's dextrose agar containing cycloheximide with olive oil overlay and modified Dixon agar and by doing biochemical tests (catalase reaction, assimilation of glycine, and Tween utilisation tests).


RESULTS
In 10 patients, 10% KOH smear was negative, while in 90 patients the smear showed characteristic "spaghetti and meatball" appearance. Of these 90 cases, growth was obtained on modified Dixon's agar in 87 cases. Fifty of the isolates (57.5%) were M. globosa, 15 (17.2%) were M. sympodialis, seven (8.0%) were suspected M. sympodialis, 6 (6.9%) each of the isolates were M. furfur and M. obtusa, and three (3.4%) isolates were M. restricta.


CONCLUSIONS
M. globosa was the most common species, followed by M. sympodialis, M. furfur, M. obtusa, and M. restricta.
Chaudhary
Rahul
R
Department of Dermatology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221 005, India.
Singh
Sanjay
S
Banerjee
Tuhina
T
Tilak
Ragini
R
D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D008290:Malassezia / Q000302:isolation & purification; D008297:Male; D015995:Prevalence; D013045:Species Specificity; D014010:Tinea Versicolor / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D055815:Young Adult