Mitochondrial evidence for multiple radiations in the evolutionary history of small apes.
BACKGROUND
Gibbons or small apes inhabit tropical and subtropical rain forests in Southeast Asia and adjacent regions, and are, next to great apes, our closest living relatives. With up to 16 species, gibbons form the most diverse group of living hominoids, but the number of taxa, their phylogenetic relationships and their phylogeography is controversial. To further the discussion of these issues we analyzed the complete mitochondrial cytochrome b gene from 85 individuals representing all gibbon species, including most subspecies.


RESULTS
Based on phylogenetic tree reconstructions, several monophyletic clades were detected, corresponding to genera, species and subspecies. A significantly supported branching pattern was obtained for members of the genus Nomascus but not for the genus Hylobates. The phylogenetic relationships among the four genera were also not well resolved. Nevertheless, the new data permitted the estimation of divergence ages for all taxa for the first time and showed that most lineages emerged during four short time periods. In the first, between approximately 6.7 and approximately 8.3 mya, the four gibbon genera diverged from each other. In the second (approximately 3.0 - approximately 3.9 mya) and in the third period (approximately 1.3 - approximately 1.8 mya), Hylobates and Hoolock differentiated. Finally, between approximately 0.5 and approximately 1.1 mya, Hylobates lar diverged into subspecies. In contrast, differentiation of Nomascus into species and subspecies was a continuous and prolonged process lasting from approximately 4.2 until approximately 0.4 mya.


CONCLUSIONS
Although relationships among gibbon taxa on various levels remain unresolved, the present study provides a more complete view of the evolutionary and biogeographic history of the hylobatid family, and a more solid genetic basis for the taxonomic classification of the surviving taxa. We also show that mtDNA constitutes a useful marker for the accurate identification of individual gibbons, a tool which is urgently required to locate hunting hotspots and select individuals for captive breeding programs. Further studies including nuclear sequence data are necessary to completely understand the phylogeny and phylogeography of gibbons.
Thinh
Van Ngoc
VN
Primate Genetics Laboratory, German Primate Center, Kellnerweg 4, 37077 Göttingen, Germany. vanthinhngoc@yahoo.com
Mootnick
Alan R
AR
Geissmann
Thomas
T
Li
Ming
M
Ziegler
Thomas
T
Agil
Muhammad
M
Moisson
Pierre
P
Nadler
Tilo
T
Walter
Lutz
L
Roos
Christian
C
D000818:Animals; D001210:Asia, Southeastern; D005075:Biological Evolution; D004272:DNA, Mitochondrial / Q000235:genetics*; D056727:Endangered Species; D006922:Hylobates / Q000145:classification / Q000235:genetics*; D010802:Phylogeny
D004272:DNA, Mitochondrial