NaCl effect on the distribution of wall ingrowth polymers and arabinogalactan proteins in type A transfer cells of Medicago sativa Gabès leaves.
We studied the distribution of wall ingrowth (WI) polymers by probing thin sections of companion cells specialized as transfer cells in minor veins of Medicago sativa cv Gabès blade with affinity probes and antibodies specific to polysaccharides and glycoproteins. The wall polymers in the controls were similar in WIs and in the primary wall but differently distributed. The extent of labeling in these papillate WIs differed for JIM5 and JIM7 homogalacturonans but was in the same range for LM5 and LM6 rhamnogalacturonans and xyloglucans. These data show that WI enhancement probably requires arabinogalactan proteins (JIM8) mainly localized on the outer part of the primary wall and WIs. By comparison, NaCl-treated plants exhibited cell wall polysaccharide modifications indicating (1) an increase in unesterified homogalacturonans (JIM5), probably implicated in Na(+) binding and/or polysaccharide network interaction for limiting turgor variations in mesophyll cells; (2) enhancement of the xyloglucan network with an accumulation of fucosylated xyloglucans (CCRC-M1) known to increase the capacity of cellulose binding; and (3) specific recognition of JIM8 arabinogalactan proteins that could participate in both wall enlargement and cohesion by increasing the number of molecular interactions with the other polymers. In conclusion, the cell wall polysaccharide distribution in enlarged WIs might (1) participate in wall resistance to sequestration of Na(+), allowing a better control of hydric homeostasis in mesophyll cells to maintain metabolic activity in source leaves, and (2) maintain tolerance of M. sativa to NaCl.
Boughanmi
Néziha
N
Département des Sciences de la Vie, Faculté des Sciences de Bizerte, 7021, Zarzouna, Bizerte, Tunisia. nezighanem@yahoo.fr
Thibault
Florence
F
Decou
Raphael
R
Fleurat-Lessard
Pierrette
P
Béré
Emile
E
Costa
Guy
G
Lhernould
Sabine
S
D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D000939:Epitopes / Q000648:ultrastructure; D005936:Glucans / Q000648:ultrastructure; D007150:Immunohistochemistry; D000455:Medicago sativa / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D009088:Mucoproteins / Q000378:metabolism*; D010368:Pectins / Q000648:ultrastructure; D018515:Plant Leaves / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010940:Plant Proteins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D014990:Xylans / Q000648:ultrastructure
D000939:Epitopes; D005936:Glucans; D009088:Mucoproteins; D010940:Plant Proteins; D011134:Polysaccharides; D014990:Xylans; C006619:arabinogalactan proteins; C042491:rhamnogalacturonan I; C029353:xyloglucan; D012965:Sodium Chloride; D010368:Pectins