SiRNA against Fabp5 induces 3T3-L1 cells apoptosis during adipocytic induction.
Fatty acid-binding protein 5 (Fabp5), exhibits an important role in binding free fatty acids, as well as regulating lipid metabolism and transport. The purpose of this study was to evaluate the role of Fabp5 during adipogenesis. 3T3-L1 preadipocytes were selected as cell differentiation model and short interfering RNAs (siRNA) against Fabp5 (siFabp5) were prepared. Our results showed that two potent siFabp5 specifically inhibited endogenous expression of Fabp5 at both mRNA and protein level. SiFabp5 had little effect on undifferentiated 3T3-L1 fibroblasts. However, during adipocytic induction, 3T3-L1 preadipocytes transfected with siFabp5 significantly reduced cell viability, as well as increased both caspase-3 activity and procaspase-3 cleavage. Furthermore, we illustrated that knockdown Fabp5 inhibited the expression of PPARγ and C/EBPα during adipocytic induction. In conclusion, our data suggests that Fabp5 is crucial in maintaining the viability of preadipocytes during adipogenesis via the activation of Akt cascade, and decreased Fabp5 expression induce differentiating preadipocytes apoptosis via caspase-3 activation.
State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, China. maxi@cau.edu.cn
Xi
X
Ma
Xia
X
Ren
Pengfei
P
Han
Shengdi
S
Hu
Junjun
J
Wang
Jingdong
J
Yin
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002470:Cell Survival; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D014162:Transfection
D022763:CCAAT-Enhancer-Binding Protein-alpha; C082667:Fabp5 protein, mouse; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D047495:PPAR gamma; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D053148:Caspase 3