Cytological dissection and molecular characterization of chromosome 1R derived from 'Burgas 2' common wheat.
Rye chromosome 1R harbors many agronomically important genes such as resistance genes for rusts. Using the gametocidal system, we dissected the 1R chromosome substituted for wheat chromosome 1B in a common wheat cultivar 'Burgas 2'. The gametocidal system induces chromosomal breakage in the 1R chromosome, as well as in wheat chromosomes. We cytologically examined a pool of prescreened common wheat plants that had been shown to have single or multiple rearranged 1R chromosomes and established 95 common wheat lines carrying single 1R segments. We conducted PCR analysis of these lines, termed '1R dissection lines', using 10 PCR-based 1R-specific markers. We mapped the 10 PCR-based markers along the 1R chromosome with the breakpoints of the 1R dissection lines. Based on the PCR result and the positions of the primary and secondary constrictions, we could separate the breakpoints of the rearranged 1R chromosomes into 12 regions along the 1R chromosome. On the other hand, using the breakpoints, we could separate the PCR-based markers from each other except for two markers. These dissection lines are useful in mapping DNA markers and may facilitate the construction of contig maps.
Gyawali
Yadav Prasad
YP
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Nasuda
Shuhei
S
Endo
Takashi R
TR
D019457:Chromosome Breakage; D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D013045:Species Specificity; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*
D005819:Genetic Markers