Preparative isolation and purification of anti-tumor agent ansamitocin P-3 from fermentation broth of Actinosynnema pretiosum using high-performance counter-current chromatography.
Ansamitocin P-3 is a potent anti-tumor maytansinoid found in Actinosynnema pretiosum. However, due to the complexity of the fermentation broth of Actinomycete, how to effectively separate ansamitocin P-3 is still a challenge. In this study, both analytical and preparative high-performance counter-current chromatography were successfully used to separate and purify ansamitocin P-3 from fermentation broth. A total of 28.8 mg ansamitocin P-3 with purity of 98.4% was separated from 160 mg crude sample of fermentation broth in less than 80 min with the two-phase solvent system of hexane-ethyl acetate-methanol-water (0.6:1:0.6:1, v/v/v/v). The purity and structural identification were determined by HPLC, (1)H NMR, (13)C NMR and mass spectroscopy.
Yao
Yuqin
Y
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province, PR China.
Cheng
Zhihui
Z
Ye
Haoyu
H
Xie
Yongmei
Y
He
Jing
J
Tang
Minghai
M
Shen
Tao
T
Wang
Jiangman
J
Zhou
Yan
Y
Lu
Zejun
Z
Luo
Feng
F
Chen
Lijuan
L
Yu
Luoting
L
Yang
Jin-Liang
JL
Peng
Aihua
A
Wei
Yuquan
Y
D000970:Antineoplastic Agents / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D003470:Culture Media; D005285:Fermentation; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008453:Maytansine / Q000031:analogs & derivatives* / Q000302:isolation & purification
D000970:Antineoplastic Agents; D003470:Culture Media; D008453:Maytansine; C015682:ansamitocins