Pentadecapeptide BPC 157 (PL 14736) improves ligament healing in the rat.
We improved medial collateral ligament (MCL) healing throughout 90 days after surgical transection. We introduced intraperitoneal, per-oral (in drinking water) and topical (thin cream layer) peptide therapy always given alone, without a carrier. Previously, as an effective peptide therapy, stable gastric pentadecapeptide BPC 157 (GEPPPGKPADDAGLV, an anti-ulcer peptide effective in inflammatory bowel disease therapy (PL 14736)) particularly improved healing of transected tendon and muscle and wound healing effect including the expression of the early growth response 1 (egr-1) gene. After MCL transection BPC 157 was effective in rats when given once daily intraperitoneally (10 microg or 10 ng/kg) or locally as a thin layer (1.0 microg dissolved in distilled water/g commercial neutral cream) at the site of injury, first application 30 min after surgery and the final application 24 h before sacrifice. Likewise, BPC 157 was effective given per-orally (0.16 microg/ml in the drinking water (12 ml/day/rat)) until sacrifice. Commonly, BPC 157 microg-ng-rats exhibited consistent functional, biomechanical, macroscopic and histological healing improvements. Thus, we suggest BPC 157 improved healing of acute ligament injuries in further ligament therapy.
University Hospital of Traumatology, Zagreb, Croatia.
Tomislav
T
Cerovecki
Ivan
I
Bojanic
Luka
L
Brcic
Bozo
B
Radic
Ivan
I
Vukoja
Sven
S
Seiwerth
Predrag
P
Sikiric
D000284:Administration, Oral; D000818:Animals; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D003286:Contracture / Q000517:prevention & control; D004195:Disease Models, Animal; D008297:Male; D017888:Medial Collateral Ligament, Knee / Q000187:drug effects* / Q000293:injuries* / Q000502:physiology; D009043:Motor Activity / Q000187:drug effects; D009504:Neutrophils / Q000502:physiology; D010446:Peptide Fragments / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
D024042:Collagen Type I; D024061:Collagen Type III; D010446:Peptide Fragments; D011506:Proteins; C083833:BPC 157