Lentiviral vector transduction of dendritic cells for novel vaccine strategies.
Dendritic cells (DCs) are key antigen-presenting cells that induce primary and memory immune response. Patients with chronic infections or cancer often display DC dysfunctions. Modification of DCs or DC progenitors in vitro may overcome the problems with defective DCs in vivo. Lentiviral vector is highly efficient in transducing hematopoietic cells including DCs. Examples of lentiviral modification of DCs with immune modulatory genes and analysis of antigen-specific T cells to demonstrate enhanced immune effector functions of DCs will be introduced.
Chang
Lung-Ji
LJ
Department of Molecular Genetics and Microbiology, Powell Gene Therapy Center and McKnight Brain Institute, College of Medicine, University of Florida, Gainesville, FL, USA. lchang@college.med.ufl.edu
D000818:Animals; D003713:Dendritic Cells / Q000276:immunology*; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D014161:Transduction, Genetic; D014612:Vaccines / Q000276:immunology*
D014612:Vaccines