Plasmid-associated bacteriocin production by Lactobacillus LMG21688 suppresses Listeria monocytogenes growth rebound in a food system.
Bacteriocin produced by Lactobacillus curvatus CWBI-B28wt is not completely effective against Listeria monocytogenes in food models. There is evidence suggesting that bacteriocin-degrading proteolytic enzymes produced by the CWBI-B28wt strain and/or present in the food matrix contribute to this rebound of Listeria growth. To limit this problem, we have partially characterized an approximately 10-kb plasmid responsible for bacteriocin production in L. curvatus CWBI-B28wt. This plasmid was transferred by high-voltage electroporation into a less proteolytic, but technologically competent Lactobacillus strain. When the transformed strain was used as a starter culture in a model food system, a high bacteriocin level was maintained for a longer time than with CWBI-B28wt, and Listeria growth rebound was delayed by 2 weeks (occurring after 3 weeks of apparently total inhibition, instead of one).
Kouakou
Privat
P
Faculté Universitaire des Sciences Agronomiques de Gembloux, Gembloux, Belgium. bioindus@fsagx.ac.be
Dortu
Carine
C
Dubois-Dauphin
Robin
R
Vandenbol
Micheline
M
Thonart
Philippe
P
D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics*; D015169:Colony Count, Microbial; D018274:Electroporation; D005516:Food Microbiology; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development*; D010447:Peptide Hydrolases / Q000378:metabolism; D010957:Plasmids; D013997:Time Factors; D014169:Transformation, Bacterial
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D010447:Peptide Hydrolases