Effect of dual-cured adhesive resin cements on cell proliferation of pulp and human fibroblasts.
OBJECTIVE
To comparatively evaluate the effects of three dual activated adhesive resin cements on cell proliferation of rat pulp cells (RPC-C2A) and human lung fibroblasts (MRC5).


METHODS
The cements tested were RelyX ARC, RelyX Unicem and Panavia F. The cements were prepared according to manufacturers' instructions and placed in contact with the cells. Cell survival was estimated by the sulphorhodamine-B staining assay after 24 and 72 hours and cellular changes in morphology were examined under microscope.


RESULTS
All resin cements decreased cell proliferation. The decrease observed was material- and time-dependent. Panavia F was found more potent in decreasing cell proliferation. Differences were found in the effect on cell proliferation among the materials tested, that might be associated to their clinical behavior.
Koulaouzidou
Elisabeth A
EA
Department of Operative Dentistry, School of Dentistry, Aristotle University of Thessaloniki, Greece. koulaouz@dent.auth.gr
Helvatjoglou-Antoniades
Maria
M
Palaghias
Georgios
G
Antoniades
Dimitrios
D
D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity; D017438:Bisphenol A-Glycidyl Methacrylate / Q000633:toxicity; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects*; D005456:Fluorescent Dyes; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects; D011092:Polyethylene Glycols / Q000633:toxicity; D011109:Polymethacrylic Acids / Q000633:toxicity; D051381:Rats; D019279:Resin Cements / Q000633:toxicity*; D012235:Rhodamines; D013997:Time Factors; D014343:Trypan Blue
D001672:Biocompatible Materials; D004396:Coloring Agents; D005456:Fluorescent Dyes; C438843:Panavia-Fluoro; D011109:Polymethacrylic Acids; C483701:Rely X Unicem; C462240:RelyX ARC; D019279:Resin Cements; D012235:Rhodamines; C022027:lissamine rhodamine B; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate; D014343:Trypan Blue