Single laboratory validation of a surface plasmon resonance biosensor screening method for paralytic shellfish poisoning toxins.
A research element of the European Union (EU) sixth Framework project BioCop focused on the development of a surface plasmon resonance (SPR) biosensor assay for the detection of paralytic shellfish poisoning (PSP) toxins in shellfish as an alternative to the increasingly ethically unacceptable mouse bioassay. A biosensor assay was developed using both a saxitoxin binding protein and chip surface in tandem with a highly efficient simple extraction procedure. The present report describes the single laboratory validation of this immunological screening method, for this complex group of toxins with differing toxicities, according to the European Decision 2002/657/EC in conjunction with IUPAC and AOAC single laboratory validation guidelines. The different performance characteristics (detection capability CCbeta, specificity/selectivity, repeatability, reproducibility, stability, and applicability) were determined in relation to the EU regulatory limit of 800 microg of saxitoxin equivalents (STX eq) per kg of shellfish meat. The detection capability CCbeta was calculated to be 120 microg/kg. Intra-assay repeatability was found to be between 2.5 and 12.3% and interassay reproducibility was between 6.1 and 15.2% for different shellfish matrices. Natural samples were also evaluated and the resultant data displayed overall agreements of 96 and 92% with that of the existing AOAC approved methods of mouse bioassay (MBA) and high performance liquid chromatography (HPLC), respectively.
Campbell
Katrina
K
Institute of Agri-Food and Land Use (IAFLU), School of Biological Sciences, Queen's University Belfast, David Keir Building, Stranmillis Road, Belfast, Northern Ireland, BT9 5AG. katrina.campbell@qub.ac.uk
Haughey
Simon A
SA
van den Top
Hester
H
van Egmond
Hans
H
Vilariño
Natalia
N
Botana
Luis M
LM
Elliott
Christopher T
CT
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D049872:Bivalvia / Q000737:chemistry; D049873:Cardiidae / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry; D008387:Marine Toxins / Q000032:analysis / Q000737:chemistry*; D012530:Saxitoxin / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D057096:Shellfish Poisoning; D020349:Surface Plasmon Resonance / Q000379:methods*
D000906:Antibodies; D002352:Carrier Proteins; D008387:Marine Toxins; D012530:Saxitoxin