Gangjinia marincola gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae.
A novel strictly aerobic, orange-pigmented, Gram-stain-negative bacterium, designated strain GJ16(T), was isolated from coastal seawater of Gangjin Bay, the southernmost part of the Korean peninsula, and subjected to a polyphasic taxonomic study. It grew optimally at 25-30 °C, at pH 7.0-8.0 and in the presence of 3 % NaCl. Comparative 16S rRNA gene sequence analysis revealed that strain GJ16(T) formed a distinct lineage within the family Flavobacteriaceae and shared less than 91.2 % 16S rRNA gene sequence similarity with members of the genera Leptobacterium, Zhouia, Winogradskyella, Dokdonia and Krokinobacter. The predominant cellular fatty acids were iso-C(15 : 0) (40.2 %), iso-C(15 : 1) G (12.8 %), iso-C(17 : 0) 3-OH (11.2 %) and C(15 : 0) (6.6 %). The G+C content of the genomic DNA was 39.4 mol% and the major respiratory isoprenoid quinone was MK-6. On the basis of phenotypic and genotypic data, strain GJ16(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Gangjinia marincola gen. nov., sp. nov. is proposed; the type strain is GJ16(T) (=KCTC 22649(T) =JCM 16082(T)).
Lee
Dong-Heon
DH
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
Moon
Sung-Ran
SR
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
Park
Young-Hyun
YH
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
Lee
Young Sun
YS
Department of Biology, Sunchon National University, Sunchon 540-742, Republic of Korea.
Jung
Jae Sung
JS
Department of Biology, Sunchon National University, Sunchon 540-742, Republic of Korea.
Kim
Jung Ho
JH
Department of Biological Environment, Sunchon National University, Sunchon 540-742, Republic of Korea.
Kim
Hoon
H
Department of Biological Environment, Sunchon National University, Sunchon 540-742, Republic of Korea.
Kahng
Hyung-Yeel
HY
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6