Deferasirox decreases age-associated iron accumulation in the aging F344XBN rat heart and liver.
It is thought that aging in rats and humans is associated with increases in iron accumulation and cell apoptosis. Here, we examine the relationship between cardiac iron levels and apoptosis in aged F344XBN rats that had been treated with an oral iron chelator (Deferasirox; 100 mg/kg body weight) on alternate days for 6 months. Compared to adult animals (6 month), cardiac iron (+72%), liver iron (+87%), ferritin light chain (+59%), divalent metal transporter-1 (+56%) and the number of TdT-mediated dUTP nick end labeling (TUNEL) positive cells (4.3 fold increase) were higher in 33-month-old animals (P < 0.05). Deferasirox treatment decreased cardiac iron levels by 37% (P < 0.05), and this was associated with decreases in the number of TUNEL-positive cells. Age-associated increases in cell death were coupled with increases in Bax to Bcl-2 ratio, and the amount of Bad, full-length caspase-3, and cleaved caspase-3. Deferasirox treatment decreased the Bax to Bcl-2 ratio by 17% (P < 0.05) and the amount of Bad, full-length caspase-3, cleaved caspase-3 (19 kDa), and cleaved caspase-3 (17 kDa) by 41, 16, 22, and 37%, respectively (P < 0.05). Taken together, these data suggest that deferasirox may be effective in diminishing age-associated iron accumulation and cardiac apoptosis in the F344XBN rat model.
Arvapalli
Ravi Kumar
RK
Department of Biological Sciences, Marshall University, Huntington, WV 25755, USA.
Paturi
Satyanarayana
S
Laurino
Joseph P
JP
Katta
Anjaiah
A
Kakarla
Sunil K
SK
Gadde
Murali K
MK
Wu
Miaozong
M
Rice
Kevin M
KM
Walker
Ernest M
EM
Wehner
Paulette
P
Blough
Eric R
ER
D000375:Aging / Q000187:drug effects*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001565:Benzoates / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D000077588:Deferasirox; D006321:Heart / Q000187:drug effects*; D020287:In Situ Nick-End Labeling; D007501:Iron / Q000032:analysis / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000494:pharmacology*; D019190:Iron Overload / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D014131:Trace Elements / Q000032:analysis; D014230:Triazoles / Q000494:pharmacology*
D001565:Benzoates; D007502:Iron Chelating Agents; D014131:Trace Elements; D014230:Triazoles; D007501:Iron; D000077588:Deferasirox