[Expression of c-jun during the incised wound healing in mice skin].
OBJECTIVE To investigate the time-dependent expression of c-jun during the healing of incised wound in mice skin.


METHODS
The expression of c-jun in different stages after the incised wound were detected by immunohistochemistry and Western blot.


RESULTS
There was a low level expression of c-jun in normal mice skin. Expression of c-jun was mainly detected in neutrophils from 3 h to 12h after injury. The c-jun positive cells were almost mononuclear cells (MNCs) and fibroblasts between 1 d and 5 d after injury. The c-jun positive cells were mostly fibroblasts between 7 d and 14 d after injury. The ratio of the c-jun positive cells increased in the wound specimens from 3 h to 12 h, peaked at 12 h, declined partially from 1 d to 5 d, and reached the peak secondly at 7 d, then decreased from 10 d to 14 d. The expression of c-jun was observed throughout the wound healing stages by Western blot with two peaks occurring at 12 h and 7 d after injury.


CONCLUSIONS
The c-jun may play a potential role in inducing apoptosis of neutrophils, MNCs and fibroblasts during skin wound healing, and it may be used as the marker for wound age determination.
Yang
Me
M
School of Forensic Medicine, China Medical University, Shenyang 110001, China. ymdoctor@163.com
Guan
Da-Wei
DW
Xiong
Chang-Yan
CY
Cheng
Zi-Hui
ZH
Yu
Tian-Shui
TS
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D004195:Disease Models, Animal; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D011897:Random Allocation; D012867:Skin / Q000293:injuries* / Q000378:metabolism*; D013997:Time Factors; D014945:Wound Healing; D014947:Wounds and Injuries / Q000378:metabolism
D016755:Proto-Oncogene Proteins c-jun