Molecular characterization of Hepatitis A virus causing an outbreak among Thai navy recruits.
Hepatitis A virus (HAV) infection is a communicable disease, typically transmitted by faecal-oral contamination. HAV outbreaks usually occur in endemic areas. We report an outbreak of HAV from June to July, 2008 among Thai navy recruits who had received training at the Sattahip Navy Base, Chonburi province, Thailand. Upon conclusion of the training, the recruits were deployed to serve at several navy bases across the country. Secondary cases of HAV infection were reported among military personnel from these navy bases. To elucidate origin and distribution of these outbreaks, we characterized the genome and genotype of HAV isolated from the different navy bases. Sera and stool from the subjects were tested for antiHAV IgM, antiHAV IgG and HAV RNA. Subsequently, molecular characterization of HAV was performed by nucleotide sequencing of the VP1-P2A region, BLAST/FASTA and phylogenetic analysis. HAV RNA was detected in specimens obtained from different areas. All isolated strains clustered in the same lineage and belonged to genotype 1A. They shared nearly 100% genome homology indicating a single point source of this outbreak. This study provides essential baseline data as a reference for genetic analysis of HAV strains causing future outbreaks. Early detection of HAV infection and identification of the source by using molecular characterization and prompt preventive measures will hopefully prevent further outbreaks.
Center of Excellence in Viral Hepatitis, Paediatric Department, Faculty of Medicine, Chulalongkorn University and Hospital, Bangkok, Thailand.
A
A
Theamboonlers
P
P
Rianthavorn
S
S
Jiamsiri
S
S
Kumthong
P
P
Silaporn
C
C
Thongmee
Y
Y
Poovorawan
D000328:Adult; D001483:Base Sequence; D004196:Disease Outbreaks; D005243:Feces; D005838:Genotype; D006506:Hepatitis A / Q000175:diagnosis / Q000453:epidemiology* / Q000635:transmission / Q000821:virology*; D035922:Hepatitis A Antibodies / Q000097:blood; D017957:Hepatitis A Virus, Human / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D008889:Military Personnel; D025202:Molecular Diagnostic Techniques; D017720:Molecular Epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013785:Thailand / Q000453:epidemiology; D015678:Viral Structural Proteins / Q000235:genetics
D035922:Hepatitis A Antibodies; D012367:RNA, Viral; D015678:Viral Structural Proteins