Time-series gene expression profiles in AGS cells stimulated with Helicobacter pylori.
OBJECTIVE
To extend the knowledge of the dynamic interaction between Helicobacter pylori (H. pylori) and host mucosa.


METHODS
A time-series cDNA microarray was performed in order to detect the temporal gene expression profiles of human gastric epithelial adenocarcinoma cells infected with H. pylori. Six time points were selected to observe the changes in the model. A differential expression profile at each time point was obtained by comparing the microarray signal value with that of 0 h. Real-time polymerase chain reaction was subsequently performed to evaluate the data quality.


RESULTS
We found a diversity of gene expression patterns at different time points and identified a group of genes whose expression levels were significantly correlated with several important immune response and tumor related pathways.


CONCLUSIONS
Early infection may trigger some important pathways and may impact the outcome of the infection.
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, PO Box 5, Changping District, Beijing 102206, China.
Yuan-Hai
YH
You
Yan-Yan
YY
Song
Fan-Liang
FL
Meng
Li-Hua
LH
He
Mao-Jun
MJ
Zhang
Xiao-Mei
XM
Yan
Jian-Zhong
JZ
Zhang
D045744:Cell Line, Tumor; D016000:Cluster Analysis; D005753:Gastric Mucosa / Q000382:microbiology / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D016480:Helicobacter pylori / Q000472:pathogenicity*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D013274:Stomach Neoplasms / Q000235:genetics / Q000382:microbiology; D013997:Time Factors