A rapid assay for detecting antibody against Bluetongue virus with a latex agglutination test using recombinant VP7 antigen.
A latex agglutination test (LAT) for detecting antibody against Bluetongue virus (BTV) in ruminants was developed using latex beads coupled with recombinant VP7 protein. Compared with competitive enzyme-linked immunosorbent assay (ELISA), the specificity and sensitivity of the LAT were 99.0% and 93.0%, respectively. There was excellent agreement between the results obtained by competitive ELISA and the LAT (kappa = 0.930). Because it is rapid and easy to use, the LAT could be used for BTV antibody detection, especially for screening many serum samples.
Yang
Junxing
J
No. 2049, Heping Road, Shenzhen, Guangdong, P. R. China 518001. ciqhua@yahoo.com.cn
Hua
Qunyi
Q
Chen
Huanchun
H
Lv
Jianqiang
J
Chen
Bing
B
Ruan
Zhouxi
Z
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001819:Bluetongue / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D001820:Bluetongue virus / Q000276:immunology*; D007841:Latex Fixation Tests / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012756:Sheep; D014758:Viral Core Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D014758:Viral Core Proteins; C066862:VP7 protein, orbivirus