New method to produce hemocomponents for regenerative use from peripheral blood: integration among platelet growth factors monocytes and stem cells.
Recent studies have shown the importance of monocytes/macrophageses and of CD34+ progenitors in tissue regeneration processes. These cells, obtained generally from bone marrow, are seen in damaged tissue. We have studied a method to collect from the peripheral blood, using a cell separator and without stimulation of the patient/donor, a leukocyte platelet concentrated hemocomponent (CLP) for regenerative use which contains platelets, monocytes/macrophages, fibrinogen and CD34+ cells. We appraised the composition and cell functionality of the final hemocomponent during production and cryoconservation. The results show a positive increase in concentration values, in comparison with the pre-collection, of the cells that were involved in regeneration; i.e. the platelets, monocytes and CD34+ cells. These concentrations were also maintained at an effective level during cryoconservation of the hemocomponent. The CLP also demonstrated positive clonogenic potential in culture, showing that the CD34+ progenitors involved in CFU formation are functional in the fresh and thawed product. In brief we have shown that it is possible to produce, in a simple way, a hemocomponent for regenerative use that is standardized, reliable, and is economically feasible.
Dipartimento di Immunoematologia e Medicina Trasfusionale, Ospedale San Martino, viale Europa 22, 32100 Belluno, Italy. gaetano.caloprisco@ulss.belluno.it
Gaetano
G
Caloprisco
Alessio
A
Borean
Sergio
S
De Angeli
Giovanni Battista
GB
Gaio
Katia
K
Boito
Laura
L
Del Pup
Elisabetta
E
Pavan
Valentina
V
Casale
Ivone
I
Varinelli
D018952:Antigens, CD34 / Q000032:analysis; D001781:Blood Component Removal / Q000191:economics / Q000379:methods* / Q000592:standards; D002469:Cell Separation / Q000191:economics / Q000379:methods* / Q000592:standards; D006801:Humans; D008297:Male; D009000:Monocytes / Q000166:cytology*; D010982:Platelet-Derived Growth Factor / Q000032:analysis*; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology*
D018952:Antigens, CD34; D010982:Platelet-Derived Growth Factor