[Neurotoxicity effect of MPP+ on primary cultured mesencephalon neurons of SAMP8 mouse].
OBJECTIVE
To investigate the effect of MPP+ on the midbrain neurons of SAMP8 mouse in vitro, which provide the cell model for PD study.


METHODS
SAMP8 mouse primary midbrain cell cultures were obtained from mice within 1 day after birth. On the sixth day after culturing, the cultures were treated with 100 micromol/L of MPP+ for 6 hours, 9 hours, 12 hours and 24 hours, then the cells were fixed and followed by immunofluorescence or Western blot analysis for the expressed location and the level of TH respectively.


RESULTS
MPP+ led to the morphological changes of primary cultured midbrain neurons of SAMP8 mouse. The neurons had intact morphous with strong immunoreactivity, many and long dendrites in control group. Compared with the control group, the neurons had fewer and thinness dendrites with weak immunoreactivity in MPP+ group. MPP+ significantly decreased midbrain neurons numbers with marked decrease of TH protein levels after its treatment for 9 h.


CONCLUSIONS
MPP+ had neurotoxicity effect on primary cultured midbrain neurons of SAMP8 mouse. Since MPP+ led to marked decrease of neuronal numbers and TH protein levels, it is suggested that MPP+ can cause the DA neuronal degeneration in primary midbrain cell cultures.
Department of Neurology, Tangshan Worker Hospital, Tangshan, China. ljljqdoc1999@yahoo.com.cn
Jing
J
Liu
Jian-qiang
JQ
Liu
Li
L
Liu
Lin
L
Ma
Yan-yong
YY
Wang
Qin-ying
QY
Ma
Ming-wei
MW
Wang
D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000375:Aging / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D008636:Mesencephalon / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D008806:Mice, Inbred AKR; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D014446:Tyrosine 3-Monooxygenase; D015655:1-Methyl-4-phenylpyridinium