Cloning and expression of fat body hexamerin receptor and its identification in other hexamerin sequestering tissue of rice moth, Corcyra cephalonica.
Selective receptor mediated uptake is a widely prevalent mechanism in insects by which important macromolecules are acquired. Among the various proteins sequestered by the insect fat body, the larval hexamerins form the major group. In the present work full length cDNA (2.6kb) of hexamerin receptor with an ORF of 2.4kb was cloned from the larval fat body of rice moth, Corcyra cephalonica. This was followed by the recombinant expression of truncated N-terminal sequence of putative hexamerin receptor and the confirmation of the expressed recombinant protein as the truncated hexamerin receptor by ligand blot analysis. Apart from this we also analyzed other hexamerin sequestering tissues like salivary gland, male accessory reproductive gland and ovary for the presence of hexamerin receptor. We found that the receptor in these tissues was similar in size and mode of activation to that of fat body hexamerin receptor, thus cementing the fact that identical hexamerin receptors are present in all the hexamerin sequestering tissues in the rice moth.
Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, Andhra Pradesh, India.
Manohar
M
Damara
Damodar
D
Gullipalli
Aparna
A
Dutta-Gupta
D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004441:Ecdysterone / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005216:Fat Body / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005835:Genitalia / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009036:Moths / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism
D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D019476:Insect Proteins; D011994:Recombinant Proteins; C092382:arylphorin-binding proteins, insect; D004441:Ecdysterone