CYP4F2 gene V433M polymorphism is associated with ischemic stroke in the male Northern Chinese Han population.
BACKGROUND
CYP4F2 is a member of the cytochrome P450 enzymes and is responsible for metabolizing arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE); 20-HETE plays a role in the regulation of vascular tone in the cerebral, coronary, and renal circulation. The present study aimed to evaluate whether or not the CYP4F2 gene polymorphism V433M (rs2108622) is involved in ischemic stroke in the Northern Chinese Han population.


METHODS
In a case-control study, the participants included 302 (193 males and 109 females) patients with ischemic stroke and 350 (212 males and 138 females) healthy subjects. The V433M polymorphism of the CYP4F2 gene was analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) with the restriction enzyme PvuII.


RESULTS
The frequencies of the GG genotype and the G allele were higher in participants with ischemic stroke than in the control group (P=0.018). Multiple logistic regression analysis revealed the significance of the rs2108622 in males after adjustment for confounding factors. No difference was found in all participants and females.


CONCLUSIONS
The GG genotype and G allele were associated with ischemic stroke in the male Northern Chinese Han population.
Department of Neurology, The First Affiliated Hospital of China Medical University, Shenyang 110001, China.
Shumin
S
Deng
Gang
G
Zhu
Fang
F
Liu
Hui
H
Zhang
Xue
X
Qin
Lei
L
Li
He
H
Zhiyi
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002545:Brain Ischemia / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002681:China; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D000072470:Cytochrome P450 Family 4; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012044:Regression Analysis; D012737:Sex Factors; D020521:Stroke / Q000235:genetics*
D003577:Cytochrome P-450 Enzyme System; D000072470:Cytochrome P450 Family 4; C117480:CYP4F2 protein, human