Functional analysis of pcpme6 from oomycete plant pathogen Phytophthora capsici.
The Phytophthora capsici inflicts damage on numerous crop plants by secreting a series of pectinase including pectin methylesterase (PME). We identified a PME gene (pcpme6) from a genomic library of a highly virulent P. capsici strain SD33 which had an encoded a polypeptide of 348 amino acid residues with a predicted molecular mass of 38.18 kDa. We also confirmed that pcpme6 was increasingly expressed during symptom development following P. capsici infection of pepper leaves. The wild-type protein (PCPME6) ca. 50 kDa was obtained from pcpme6 expression, and PME activity trend in PCPME6-treated pepper leaves increased with symptom development. PCPME6 degraded leaf cell walls, resulting in the production of necrotic lesions. Mutation of Asp residues in active sites within pcpme6 affected PCPME6 activity and its virulence on pepper leaves. Results show that pcpme6 is a gene within the pme gene family that is important for pathogenesis of P. capsici on pepper.
Department of Plant Pathology, Shandong Agricultural University, No. 61 Daizong street, Tai'an 271018, PR China.
Baozhen
B
Feng
Peiqian
P
Li
Hemei
H
Wang
Xiuguo
X
Zhang
D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002212:Capsicum / Q000382:microbiology; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D010838:Phytophthora / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D014774:Virulence
D020418:Algal Proteins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase