Pluripotential differentiation capability of human adipose-derived stem cells in a novel fibrin-agarose scaffold.
The potentiality of adipose-derived stem cells (ASCs) cultured on 2D systems has been previously established. Nevertheless, very little is known so far about the differentiation potentiality of ASCs in 3D culture systems using biomaterials. In this work, we have evaluated the transdifferentiation capabilities of ASCs cultured within a novel fibrin-agarose biomaterial by histological analysis, histochemistry and immunofluorescence. Our results showed that 3D fibrin-agarose biomaterial is highly biocompatible and supports the transdifferentiation capabilities of ASCs to the osteogenic, chondrogenic, adipogenic, and neurogenic lineages.
Faculty of Medicine, Department of Histology (Tissue Engineering Group) University of Granada, Avenida de Madrid 11, Granada, Spain.
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Nieto-Aguilar
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Serrato
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Garzón
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Campos
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Alaminos
D000273:Adipose Tissue / Q000166:cytology*; D002454:Cell Differentiation; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D020219:Chondrogenesis; D005337:Fibrin / Q000737:chemistry / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D055495:Neurogenesis; D010012:Osteogenesis; D012685:Sepharose / Q000737:chemistry / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*
D005337:Fibrin; D012685:Sepharose