Isolation and characterization of nicotiflorin obtained by enzymatic hydrolysis of two precursors in tea seed extract.
Two flavonol triglycosides, camelliaside A (CamA) and camelliaside B (CamB), of tea seed extract (TSE) were subjected to enzymatic hydrolysis. Among five kinds of glycosidases investigated, beta-galactosidase (Gal) induced selective hydrolysis of CamA. On the other hand, pectinase (Pec) and cellulase (Cel) induced hydrolysis of CamB. For Gal and Pec, only kaempferol diglycoside (nicotiflorin, NF) was produced; on the other hand, significant amounts of kaempferol monoglycoside (astragalin, AS) and kaempferol (KR) were also detected for Cel. The combination of the use of Gal and Pec in the enzymatic hydrolysis of TSE afforded NF with high specificity. Crude NF with 22% purity was recovered from the enzymatic reaction mixture by extraction with organic solvent, and pure NF with >95% purity was obtained by crystallized in water. The chemical structure of NF was confirmed by (1)H and (13)C NMR analyses.
National Research Laboratory of Bioactive Materials Laboratory, Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
Hyang-Bok
HB
Lee
Eun-Ki
EK
Kim
Sang-Jae
SJ
Park
Sang-Gu
SG
Bang
Tae Gil
TG
Kim
Dae-Won
DW
Chung
D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D006868:Hydrolysis; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D013662:Tea / Q000737:chemistry*
D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D013662:Tea; C513882:nicotiflorin