Hydrodynamic conditions induce changes in secretion level and glycosylation patterns of Von Willebrand factor (vWF) in endothelial cells.
The effect of low-speed agitation on the level of secretion and glycosylation patterns of the Von Willebrand factor in endothelial cells cultured in 6-cm Petri dishes located in a rotating platform was studied. In this simple system, complex and non-intuitive velocity fields arise. As revealed by simple calculations of tangential velocities and CFD analysis, flow behavior strongly varies as a function of radial distance and rotational velocity. We found significant differences in both secretion level and glycosylation extent and completeness, as a result of relatively small changes in rotational speed. Results obtained support the hypothesis that differences in hydrodynamic conditions may induce important changes in the secretion and assembly of glycoproteins in mammalian cell cultures.
Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Ave. Eugenio Garza Sada 2501 sur. Monterrey, N.L. C.P. 64849, México.
Maritza
M
Morga-Ramírez
María Teresa
MT
Collados-Larumbe
Keith E
KE
Johnson
María José
MJ
Rivas-Arreola
Leydi Maribel
LM
Carrillo-Cocom
Mario Moisés
MM
Alvarez
D057005:Bioengineering; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D006031:Glycosylation; D018056:Hemorheology; D006801:Humans; D008954:Models, Biological; D014841:von Willebrand Factor / Q000737:chemistry / Q000378:metabolism*
D014841:von Willebrand Factor