Effects of streptozotocin-induced long-term diabetes on parietal cell function and morphology in rats.
Gastric pathology is a common complication in diabetes mellitus. The aim of the study was to evaluate the functions and morphological changes of the parietal cells of the rat stomach after streptozotocin-induced diabetes. Diabetes mellitus was induced in Wistar rats by a single intraperitoneal injection of streptozotocin (60 mg/kg body weight). The rats were weighed weekly and sacrificed after 6 months. The glandular portion of the stomach was removed and processed for H(+)-K(+)-ATPase immunohistochemistry and light and electron microscopy studies. Acid secretion was measured in vivo. After 6 months of diabetes, the mean weight of the rats was significantly lower (P < 0.001) compared to control. The mean weight of the stomach to body weight percentage increased significantly (P < 0.001) compared to control. The blood glucose level in diabetic rats was significantly higher (P < 0.001) than in normal control. Diabetic rats showed significant (P < 0.001) decrease in basal and stimulated acid secretion when compared to control. Electron micrographs of the parietal cells of glandular stomach of diabetic rats revealed significant (P < 0.0002) reduction in the number of mitochondria and a small though not significant increase in the number of canaliculi in the parietal cells compared with normal. Immunohistochemistry showed reduced H(+)-K(+)-ATPase (P < 0.00001) compared to control. Long-term diabetes induces morphological as well as functional changes in gastric parietal cells. The decrease in the number of mitochondria accompanied by reduced in H(+)-K(+)-ATPase in parietal cells may explain the reduced acid secretion observed in diabetics.
Bastaki
Salim M A
SM
Department of Pharmacology, Faculty of Medicine and Health Sciences, UAE University, PO Box 17666 Al Ain, UAE. sbastaki@uaeu.ac.ae
Adeghate
Ernest
E
Chandranath
Irwin S
IS
Amir
Naheed
N
Tariq
Saeed
S
Hameed
Rashed S
RS
Adem
Abdu
A
D000818:Animals; D001835:Body Weight; D048909:Diabetes Complications / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D005744:Gastric Acid / Q000378:metabolism; D017506:H(+)-K(+)-Exchanging ATPase / Q000032:analysis; D008928:Mitochondria; D009929:Organ Size; D010295:Parietal Cells, Gastric / Q000473:pathology* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach; D013311:Streptozocin; D013997:Time Factors
D013311:Streptozocin; D017506:H(+)-K(+)-Exchanging ATPase