An introduction to mitochondrial informatics.
In this chapter, we review the public resources available for human mitochondrial DNA and protein related bioinformatics, with a special focus on mitochondrial single nucleotide polymorphisms (mtSNPs). We also review our own freeware tool V-MitoSNP, giving an overview of its implementation and program workflow. Apart from these, we review several protocols for the graphic input of genes, keywords, gene searching by sequence, mtSNP searching by sequence, restriction enzyme mining, primer design, and virtual electrophoresis for PCR-RFLP genotyping. Some databases with similar function are integrated and compared.
Department of Biomedical Science and Environmental Biology, Center of Excellence for Environmental Medicine, Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan.
Hsueh-Wei
HW
Chang
Li-Yeh
LY
Chuang
Yu-Huei
YH
Cheng
De-Leung
DL
Gu
Hurng-Wern
HW
Huang
Cheng-Hong
CH
Yang
D019295:Computational Biology / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics; D054629:Genome, Mitochondrial; D006801:Humans; D008928:Mitochondria / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
D004272:DNA, Mitochondrial