[Expression and significance of PCNA and Caspase-3 in the tissue of lung cancer].
OBJECTIVE
To investigate the significance of PCNA and Caspase-3 in lung cancer.


METHODS
PCNA and Caspase-3 in 80 patients diagnosed with lung cancer were semi-quantitatively analyzed by using SP immunohistochemical staining.


RESULTS
The positive rate of Caspase-3 and PCNA were 32.5% and 65%, respectively. Both were much lower or higher than those normal lung tissues (P<0.05). Caspase-3 protein was correlated to lymph node metastasis, pathological type and differentiation grade (P<0.05). Meanwhile, PCNA was correlated to lymph node metastasis, differentiation grade and pathological staging of lung cancer (P<0.05), but not to pathological type. PCNA was negatively correlated to Caspase-3. PCNA (+)/Caspase-3(-) indicated the potential lower differentiation grade.


CONCLUSIONS
Up-regulation of PCNA and down-regulation of Caspase-3 are correlated to the pathological type, lymph node metastasis, staging and differentiation grade in lung cancer, especially which is indicating its clinical treatment.
Department of Thoracic Oncosurgery, the First Affiliated Hospital to Xi'an Jiaotong University, Xi'an, China. szhaoy@126.com
Yang
Y
Zhao
Xiao-jun
XJ
Li
Xin
X
Sui
Xiao-jiang
XJ
Tang
Hong
H
Qin
Hong
H
Ren
D053148:Caspase 3 / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000737:chemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology; D008207:Lymphatic Metastasis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*
D018809:Proliferating Cell Nuclear Antigen; D053148:Caspase 3