Spectral domain optical coherence tomography and adaptive optics: imaging photoreceptor layer morphology to interpret preclinical phenotypes.
Recent years have seen the emergence of advances in imaging technology that enable in vivo evaluation of the living retina. Two of the more promising techniques, spectral domain optical coherence tomography (SD-OCT) and adaptive optics (AO) fundus imaging provide complementary views of the retinal tissue. SD-OCT devices have high axial resolution, allowing assessment of retinal lamination, while the high lateral resolution of AO allows visualization of individual cells. The potential exists to use one modality to interpret results from the other. As a proof of concept, we examined the retina of a 32 year-old male, previously diagnosed with a red-green color vision defect. Previous AO imaging revealed numerous gaps throughout his cone mosaic, indicating that the structure of a subset of cones had been compromised. Whether the affected cells had completely degenerated or were simply morphologically deviant was not clear. Here an AO fundus camera was used to re-examine the retina (~6 years after initial exam) and SD-OCT to examine retinal lamination. The static nature of the cone mosaic disruption combined with the normal lamination on SD-OCT suggests that the affected cones are likely still present.
Rha
Jungtae
J
Department of Ophthalmology, Medical College of Wisconsin, The Eye Institute, Milwaukee, WI, USA.
Dubis
Adam M
AM
Wagner-Schuman
Melissa
M
Tait
Diane M
DM
Godara
Pooja
P
Schroeder
Brett
B
Stepien
Kimberly
K
Carroll
Joseph
J
D000328:Adult; D000595:Amino Acid Sequence; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D055355:Opsins / Q000737:chemistry; D055095:Optics and Photonics; D010641:Phenotype; D020419:Photoreceptor Cells, Vertebrate / Q000473:pathology*; D017949:Retinal Cone Photoreceptor Cells / Q000473:pathology; D041623:Tomography, Optical Coherence / Q000379:methods*; D055815:Young Adult
D055355:Opsins