Antioxidant and NF-κB inhibitory constituents isolated from Morchella esculenta.
Morchella esculenta Pers. (Morchellaceae) was investigated for its antioxidant activity by measuring the intracellular reactive oxygen species in HT-29 colon cancer cells. The methylene chloride extract, which showed the highest antioxidant activity, led to the isolation of four fungal sterols, 1-4, and trilinolein (5), in addition to methyl myristate (6), 1-linoleoylglycerol (7), and ceramide (8). The isolated compounds were identified through the analysis of various spectroscopic methods. In the hydroxyl radical assay, 5-dihydroergosterol exhibited significant antioxidant activity. All compounds isolated were also tested using an enzyme-based Elisa NF-κB assay. Fungal sterols and trilinolein showed significant inhibition of NF-κB activation in the NF-κB assay.
Division of Pharmacy Practice and Administration, College of Pharmacy, The Ohio State University, Columbus, OH, USA.
Jeong-Ah
JA
Kim
Edward
E
Lau
David
D
Tay
Esperanza J Carcache
EJ
De Blanco
D000975:Antioxidants / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D019073:HT29 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D000975:Antioxidants; D016328:NF-kappa B; D017382:Reactive Oxygen Species