[Inhibitor of tyrosine phosphorylation AG490 inhibits the proliferation of Jurkat T cells].
OBJECTIVE
To study effect of AG490 on the activation, proliferation, cell cycle, apoptosis and the expression of protein ICBP90 of Jurkat T cells. To explore the possibility of inhibiting and the initial growth mechanism of Jurkat T cells by blocking JAK signaling pathway.


METHODS
The expression level of CD69 and CD25 on the surface of Jurkat T cells was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique. The proliferation of Jurkat T cells was analyzed by MTT method. The cycle of Jurkat T cell was analyzed by PI staining and Flow Cytometry. The apoptosis of Jurkat T cells was analyzed by Annexin V-FITC and PI staining. The expression of ICBP90 that correlated with the cell proliferation was checked by Western blot.


RESULTS
The results came out that the proliferation capacity of Jurkat T cells was decreased with the increasing of AG490 ranging from 1 mmol/L to 30 mmol/L in a dose-dependent manner with the highest inhibitory rate of 27.37%. Cell-cycle analysis revealed that AG490 arrest it in G0/G1 phase and inhibited its entrance into S and G2/M phase, and reduced the expression of protein ICBP90, especially in the 24 h treatment. But the apoptosis of Jurkat T cells was not promoted by AG490.


CONCLUSIONS
AG490 significantly inhibited the growth of Jurkat T cells, and its inhibition may reduce the expression of protein ICBP90, which was related to the cell cycle arrest rather than apoptosis.
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou, China. mcq2266580@163.com
Cheng-quan
CQ
Mao
Fei-yue
FY
Xing
Zhong-feng
ZF
Guo
Zhi-yuan
ZY
Fang
D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells; D010766:Phosphorylation / Q000187:drug effects*; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D014443:Tyrosine / Q000378:metabolism; D020032:Tyrphostins / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; D014443:Tyrosine