Bioavailability of chlorogenic acids following acute ingestion of coffee by humans with an ileostomy.
The intestinal absorption and metabolism of 385 micromol chlorogenic acids following a single intake of 200 mL of instant coffee by human volunteers with an ileostomy was investigated. HPLC-MS(3) analysis of 0-24h post-ingestion ileal effluent revealed the presence of 274+/-28 micromol of chlorogenic acids and their metabolites accounting for 71+/-7% of intake. Of the compounds recovered, 78% comprised parent compounds initially present in the coffee, and 22% were metabolites including free and sulfated caffeic and ferulic acids. Over a 24h period after ingestion of the coffee, excretion of chlorogenic acid metabolites in urine accounted for 8+/-1% of intake, the main compounds being ferulic acid-4-O-sulfate, caffeic acid-3-O-sulfate, isoferulic acid-3-O-glucuronide and dihydrocaffeic acid-3-O-sulfate. In contrast, after drinking a similar coffee, urinary excretion by humans with an intact colon corresponded to 29+/-4% of chlorogenic acid intake. This difference was due to the excretion of higher levels of dihydroferulic acid and feruloylglycine together with sulfate and glucuronide conjugates of dihydrocaffeic and dihydroferulic acids. This highlights the importance of colonic metabolism. Comparison of the data obtained in the current study with that of Stalmach et al. facilitated elucidation of the pathways involved in post-ingestion metabolism of chlorogenic acids and also helped distinguish between compounds absorbed in the small and the large intestine.
Plant Products and Human Nutrition Group, Graham Kerr Building, Faculty of Medicine, University of Glasgow, Glasgow G128QQ, UK.
Angélique
A
Stalmach
Heike
H
Steiling
Gary
G
Williamson
Alan
A
Crozier
D000328:Adult; D001682:Biological Availability; D002109:Caffeic Acids / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine; D002726:Chlorogenic Acid / Q000737:chemistry / Q000493:pharmacokinetics* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003069:Coffee / Q000737:chemistry*; D003373:Coumaric Acids / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine; D005260:Female; D006801:Humans; D007081:Ileostomy; D007082:Ileum / Q000378:metabolism; D007408:Intestinal Absorption; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization
D002109:Caffeic Acids; D003069:Coffee; D003373:Coumaric Acids; D002726:Chlorogenic Acid; C004999:ferulic acid