Molecular characterization of a phospholipase C beta potentially involved in moth olfactory transduction.
To clarify the role of phospholipase C (PLC) in insect olfactory transduction, we have undertaken its molecular identification in the moth Spodoptera littoralis. From the analysis of a male antennal expressed sequence tag library, we succeeded in cloning a full-length cDNA encoding a PLC that belongs to the cluster of PLC-beta subtypes. In adult males, the PLC-beta transcript was located predominantly in brain and antennae where its presence was detected in the olfactory sensilla trichodea. Moreover, PLC-beta was expressed in antennae at the beginning of the pupal stage, then reached a maximum at the end of this stage and was maintained at this level during the adult period. Taken together, these results provided molecular evidence for the putative participation of a PLC-beta in signaling pathways responsible for the establishment and the functioning of insect olfactory system.
Unité mixte de Recherche 1272, Université Pierre et Marie Curie-Institut Nationnal de la Recherche Agronomique, Physiologie de l'Insecte, Signalisation et Communication, Université Paris VI, Bâtiment A, 7 quai Saint Bernard, 75005 Paris, France.
Bastien
B
Chouquet
Philippe
P
Lucas
Françoise
F
Bozzolan
Marthe
M
Solvar
Martine
M
Maïbèche-Coisné
Nicolas
N
Durand
Stéphane
S
Debernard
D000818:Animals; D001921:Brain / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D008297:Male; D009036:Moths; D009833:Olfactory Pathways / Q000378:metabolism*; D018034:Olfactory Receptor Neurons / Q000502:physiology*; D054799:Phospholipase C beta / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D018411:Spodoptera / Q000201:enzymology* / Q000235:genetics
D018076:DNA, Complementary; D011994:Recombinant Proteins; D054799:Phospholipase C beta