[Effects of UO-126 on proliferation and fbw7 expression of HeLa cells].
OBJECTIVE
To observe the effects of UO-126 on the expression of F-box and WD repeat domain-containing protein 7(FBW7)and on the proliferation of human cervical cancer cell lines (HeLa cells).


METHODS
HeLa cells were treated with different concentrations of UO-126, MTT assay was used to observe the proliferation of HeLa cells. Immunofluorescence showed the location and expression of FBW7 in HeLa cells. The mRNA and protein expression of FBW7 were detected by RT-PCR and Western blot before and after mitogen-activated protein kinases (MAPK)signal was blocked by UO-126 a MAPK inhibitor.


RESULTS
MTT results showed that the concentration range of MAPK signaling pathway inhibitor UO-126 inhibited the proliferation of HeLa cells in a concentration-and time-dependent manner(P<0.05). Immunofluorescence showed that the expression of positive FBW7 had increased after HeLa cells were treated with UO-126. RT-PCR and Western blot exhibited that the FBW7 mRNA and protein expression had significantly increased before and after HeLa cells were treated with UO-126(P<0.05).


CONCLUSIONS
UO-126 could inhibit HeLa cells proliferation, FBW7 lied downstream of MAPK signaling pathway.
Sun
Di
D
Department of Pathophysiology, Institute of Stem Cell and Tissue Engineering, College of Basic Medicine, Chongqing Medical University, Chongqing, China. sundi_20@163.com
Shen
Yi
Y
Wang
Shao-hua
SH
Xiang
Zi-wu
ZW
Xie
Ying-shan
YS
Jiang
Xin
X
D015153:Blotting, Western; D002070:Butadienes / Q000494:pharmacology*; D018797:Cell Cycle Proteins / Q000032:analysis / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D044783:F-Box Proteins / Q000032:analysis / Q000235:genetics*; D000073979:F-Box-WD Repeat-Containing Protein 7; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D009570:Nitriles / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044767:Ubiquitin-Protein Ligases / Q000032:analysis / Q000235:genetics*
D002070:Butadienes; D018797:Cell Cycle Proteins; D044783:F-Box Proteins; D000073979:F-Box-WD Repeat-Containing Protein 7; C513273:FBXW7 protein, human; D009570:Nitriles; D047428:Protein Kinase Inhibitors; C113580:U 0126; D044767:Ubiquitin-Protein Ligases