High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: link with an activated microenvironment in CLL disease.
Interaction of chronic lymphocytic leukemia (CLL) B cells with tissue microenvironment has been suggested to favor disease progression by promoting malignant B-cell growth. Previous work has shown expression in peripheral blood (PB) of CLL B cells of activation-induced cytidine deaminase (AID) among CLL patients with an unmutated (UM) profile of immunoglobulin genes and with ongoing class switch recombination (CSR) process. Because AID expression results from interaction with activated tissue microenvironment, we speculated whether the small subset with ongoing CSR is responsible for high levels of AID expression and could be derived from this particular microenvironment. In this work, we quantified AID expression and ongoing CSR in PB of 50 CLL patients and characterized the expression of different molecules related to microenvironment interaction. Our results show that among UM patients (1) high AID expression is restricted to the subpopulation of tumoral cells ongoing CSR; (2) this small subset expresses high levels of proliferation, antiapoptotic and progression markers (Ki-67, c-myc, Bcl-2, CD49d, and CCL3/4 chemokines). Overall, this work outlines the importance of a cellular subset in PB of UM CLL patients with a poor clinical outcome, high AID levels, and ongoing CSR, whose presence might be a hallmark of a recent contact with the microenvironment.
Palacios
Florencia
F
Unit of Recombinant Protein, Institut Pasteur de Montevideo, Montevideo, Uruguay.
Moreno
Pilar
P
Morande
Pablo
P
Abreu
Cecilia
C
Correa
Agustín
A
Porro
Valentina
V
Landoni
Ana Ines
AI
Gabus
Raul
R
Giordano
Mirta
M
Dighiero
Guillermo
G
Pritsch
Otto
O
Oppezzo
Pablo
P
D016175:B-Lymphocyte Subsets / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics; D049109:Cell Proliferation; D003564:Cytidine Deaminase / Q000097:blood* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D017578:Immunoglobulin Class Switching; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000201:enzymology* / Q000235:genetics* / Q000276:immunology; D009154:Mutation; D011379:Prognosis; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D012334:RNA, Neoplasm / Q000097:blood / Q000235:genetics; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D014408:Biomarkers, Tumor; D017931:DNA Primers; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase