[Morphology changes of ubiquitin-proteasome system in traumatic epilepsy].
OBJECTIVE
To investigate the value of ubiquitin(Ub) and ubiquitin-activating enzymel(UbE1) for the appraisement of post traumatic epilepsy (PTE).


METHODS
Fifteen specimens from human epileptic temporal cortex originating from PTE were collected as the PTE group. Fifteen specimens from non-PTE were collected as the non-PTE group. Meanwhile, 15 normal cerebral cortex specimens from people dead from acute traffic accident were collected as the control groups. Observe morphology changes of each group with HE, then with immunohistochemistry of Ub and UbE1.


RESULTS
Compared to the control group, morphology changes of neuron quantity reduction, neuron denaturation and so on were observed both in the PTE group and the non-PTE group under HE, especially in the PTE group. Ub and UbE1 mainly expressed in the nucleus and cytoplasm of the neurons in epilepsy spot without extracellular expression. The expression of Ub and UbE1 is PTE group > non-PTE group > control group (P < 0.05).


CONCLUSIONS
The neuron denaturation are one of the main pathology changes of epilepsy, and it is more obvious in the PTE group. Immunohistochemistry of Ub and UbE1 may be more helpful to distinguish PTE and non-PTE than HE staining.
Department of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China. seahe7@sina.com.cn
Peng-xiang
PX
He
Yun-lin
YL
Tang
Wei-lin
WL
Cai
Yong-hua
YH
Huang
Chao
C
Fang
Hua-lan
HL
Jing
D016022:Case-Control Studies; D002452:Cell Count; D002467:Cell Nucleus / Q000378:metabolism; D006259:Craniocerebral Trauma / Q000150:complications*; D004827:Epilepsy / Q000209:etiology / Q000473:pathology; D004834:Epilepsy, Post-Traumatic / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D049429:Forensic Pathology; D006801:Humans; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism* / Q000473:pathology*; D013194:Staining and Labeling; D025801:Ubiquitin / Q000378:metabolism*; D044764:Ubiquitin-Activating Enzymes / Q000378:metabolism*
C471538:UBA1 protein, human; D025801:Ubiquitin; D044764:Ubiquitin-Activating Enzymes