A combination method for preparing casting and transparent liver specimen.
With the development of hepatic surgery and radiology, a more accurate understanding of intrahepatic vessels and hepatic segments is necessary. Previously, research in these fields was primarily by means of dissecting livers, preparing corrosion cast specimens, reconstructing three-dimensional MSCT images of intrahepatic vessels, and so on. The aim of this study was to search for a new specimen preparing method, which could demonstrate intrahepatic vessels and the relationships between internal vessels and external structures of liver simultaneously. Rabbit livers were prepared with three different techniques in this study: (a) corrosion-cast technique; (b) transparency technique; and (c) combination technique of cast and transparency. The results showed that the combination of casting and transparent liver specimens in group C could demonstrate both the intrahepatic vessels and the external structures clearly. The relationships between the internal vessels and external structures could also be observed clearly. In conclusion, the combination method for preparing casting and transparent liver specimen created a new approach for the research of intrahepatic vessels, especially for applied basic research of hepatic segments.
Zheng
Nan
N
Department of Anatomy, Dalian Medical University, Liaoning, China.
Gong
Jin
J
Yu
Sheng-Bo
SB
Zhang
Jian-Fei
JF
Tang
Wei
W
Zhang
Cheng-Hong
CH
Bai
Jian
J
Liu
Jie
J
Sui
Hong-Jin
HJ
Shi
Bing-Yi
BY
D000818:Animals; D016319:Corrosion Casting / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008099:Liver / Q000033:anatomy & histology*; D011817:Rabbits; D013048:Specimen Handling