Expression of pigment epithelium-derived factor in human melanocytes and malignant melanoma cells and tissues: Is loss of pigment epithelium-derived factor associated with melanoma?
BACKGROUND
Pigment epithelium-derived factor (PEDF) was first isolated from the medium conditioned by human fetal retinal pigment epithelial cells and has been detected in a broad range of human fetal and adult tissues. Recent studies have indicated that PEDF activity is inhibitory to angiogenesis.


OBJECTIVE
To study the expression and distribution of pigment epithelium-derived factor (PEDF) in human melanocytes, malignant melanoma cells and tissues.


RESULTS
PEDF was expressed in human melanocytes. The expression of PEDF protein diminished in the following orders healthy skin, pigmented nevus and human malignant melanoma (p < 0.001). Both the expression of PEDF mRNA and protein was much lower or almost absent in the malignant melanoma cell line A375 than that in human melanocytes (p < 0.001).


METHODS
The expression and distribution of PEDF in human healthy skin, pigmented nevus and malignant melanoma were studied. The expression of PEDF mRNA in human melanocytes and malignant melanoma cell line A375 was measured by reverse transcription polymerase chain reaction (RT-PCR) and PEDF protein was detected by immunohistochemical method and Western blotting analysis.


CONCLUSIONS
The lack of PEDF expression may contribute to the pathogenesis of malignant melanoma.
Cheng-Feng
CF
Zhang
Lei-Hong
LH
Xiang
Jie
J
Shen
Yong
Y
Zhang
Jian
J
Li
Zhi-Zhong
ZZ
Zheng
angiogenesis; malignant melanoma; melanocytes; pigment epithelium derived factor; pigmented nevus