Increased iron stores correlate with worse disease outcomes in a mouse model of schistosomiasis infection.
Schistosomiasis is a significant parasitic infection creating disease burden throughout many of the world's developing nations. Iron deficiency anemia is also a significant health burden resulting from both nutritional deficit as well as parasitic infection in these countries. In this study we investigated the relationships between the disease outcomes of Schistosoma japonicum infection and iron homeostasis. We aimed to determine if host iron status has an effect on schistosome maturation or egg production, and to investigate the response of iron regulatory genes to chronic schistosomiasis infection. Wild-type C57BL/6 and Transferrin Receptor 2 null mice were infected with S. japonicum, and sacrificed at the onset of chronic disease. Transferrin Receptor 2 null mice are a model of type 3 hereditary hemochromatosis and develop significant iron overload providing increased iron stores at the onset of infection. The infectivity of schistosomes and egg production was assessed along with the subsequent development of granulomas and fibrosis. The response of the iron regulatory gene Hepcidin to infection and the changes in iron status were assessed by real-time PCR and Western blotting. Our results show that Hepcidin levels responded to the changing iron status of the animals, but were not significantly influenced by the inflammatory response. We also show that with increased iron availability at the time of infection there was greater development of fibrosis around granulomas. In conclusion, our studies indicate that chronic inflammation may not be the primary cause of the anemia seen in schistosomiasis, and suggest that increased availability of iron, such as through iron supplementation, may actually lead to increased disease severity.
Membrane Transport Laboratory, Division of Cancer and Cell Biology, Queensland Institute of Medical Research, Brisbane, Queensland, Australia.
Cameron J
CJ
McDonald
Malcolm K
MK
Jones
Daniel F
DF
Wallace
Lesa
L
Summerville
Sujeevi
S
Nawaratna
V Nathan
VN
Subramaniam
D000818:Animals; D015153:Blotting, Western; D005260:Female; D005786:Gene Expression Regulation; D006706:Homeostasis; D007249:Inflammation; D007501:Iron / Q000378:metabolism*; D008099:Liver / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D011990:Receptors, Transferrin / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012553:Schistosomiasis haematobia / Q000175:diagnosis* / Q000628:therapy*; D012554:Schistosomiasis japonica / Q000378:metabolism*; D016896:Treatment Outcome
D011990:Receptors, Transferrin; C503641:TFR2 protein, mouse; D007501:Iron