Injectable CMC/PEI gel as an in vivo scaffold for demineralized bone matrix.
A number of materials have been considered as sources of grafts to repair bone defects. Here, we examined the possibility of creating in situ-forming gels from sodium carboxymethylcellulose (CMC) and poly(ethyleneimine) (PEI) for use as an in vivo carrier of demineralized bone matrix (DBM). The interaction between anionic CMC and cationic PEI was examined by evaluating phase transition behavior and viscosity of CMC solutions containing 0-30 wt% PEI. CMC solutions containing 10 wt% PEI exhibited a sol-to-gel phase transition at temperatures greater than 35 degrees C. The phase transition is caused by electrostatic crosslinking of the CMC/PEI solution to form a gel with a three-dimensional network structure. In situ-formed gel implants were successfully fabricated in vivo by simple subcutaneous injection of the CMC/PEI (90/10) solution (with and without DBM) into Fisher rats. The resulting in situ-formed implant maintained its shape for 28 days in vitro and in vivo. Our results show that in situ-forming CMC/PEI gels can serve as a DBM carrier that can be delivered with a minimally invasive procedure.
Kim
Kyung Sook
KS
Fusion Biotechnology Research Center, Korea Research Institute of Chemical Technology, Yuseong, Daejeon, Korea.
Kang
Yun Mi
YM
Lee
Ju Young
JY
Kim
E Sle
ES
Kim
Chun Ho
CH
Min
Byoung Hyun
BH
Lee
Hai Bang
HB
Kim
Jae Ho
JH
Kim
Moon Suk
MS
D000818:Animals; D018775:Bone Demineralization Technique / Q000379:methods*; D001857:Bone Matrix / Q000637:transplantation*; D018786:Bone Substitutes / Q000008:administration & dosage / Q000737:chemistry*; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D007267:Injections; D008422:Materials Testing; D011094:Polyethyleneimine / Q000737:chemistry*; D051381:Rats; D011916:Rats, Inbred F344; D054457:Tissue Scaffolds
D018786:Bone Substitutes; D011094:Polyethyleneimine; D002266:Carboxymethylcellulose Sodium