Near-infrared light protect the photoreceptor from light-induced damage in rats.
BACKGROUND
A project originally developed for NASA plant growth experiments in space demonstrating the Light-Emitting Diode (LED) could promote the wound healing. Further study showed that the LED's could protect cells by stimulating the basic energy processes in the mitochondria of each cell.


OBJECTIVE
The purpose of this study was to assess the effects of 670 nm LED to protect the photoreceptor from the light-induced damage in a rodent model.


METHODS
SD rats were randomly assigned to one of eight groups: untreated control group, the LED-treated control group, three light-induced damage groups, and three LED-protected groups. The rats were exposed to constant light for 3 h of different illuminations of 900, 1,800 and 2,700 lux, respectively. The LED treatment (50 mW) were done for 30 min, 3 h before the light damage and 0, 24 and 48 h after the light damage. Using the electroretinogram as a sensitive indicator of retinal function, and the histopathologic change was showed as a proof of the protective effect of LED treatment.


RESULTS
The 900 lux illumination for 3 h did not cause damage to the retina of rats, however, the 1,800 lux illumination for 3 h caused significant damage to ONL of an approximate half retina, which caused the swing of ERG b wave to be 431 muV. With the LED protection: the damage of ONL was near 1/6 of retina, which was significantly reduced than the ones without LED protection (P < 0.01); and the swing of ERG b wave was recorded to be 1,011 muV, which was increased significantly than the ones without LED protection (P < 0.01). The illumination of 2,700 lux for 3 h caused severe damage to the rats' retinas and the LED could not protect them significantly in both of morphology and function (P > 0.05, P > 0.05).


CONCLUSIONS
670 nm LED treatment has an evident protective effect on retinal cells against light-induced damage, which may be an innovative and non-invasive therapeutic approach to prevent or to delay age-related macular degeneration.
Qu
Chao
C
Department of Ophthalmology, Sichuan Academy of Medical Sciences, Sichuan, China.
Cao
Wei
W
Fan
Yingchuan
Y
Lin
Ying
Y
D000818:Animals; D048430:Cell Shape / Q000528:radiation effects; D019610:Cytoprotection / Q000528:radiation effects*; D004581:Electronics; D004596:Electroretinography; D007259:Infrared Rays; D020419:Photoreceptor Cells, Vertebrate / Q000473:pathology* / Q000528:radiation effects*; D051381:Rats; D017207:Rats, Sprague-Dawley