Decontamination of tetramethylammonium hydroxide (TMAH) splashes: promising results with Diphoterine in vitro.
Tetramethylammonium hydroxide (TMAH), used in microelectronic industries and research and development, has both corrosive properties and systemic toxicity. Two fatal TMAH occupational exposure cases have been published. Studies comparing initial TMAH decontamination with Diphoterine versus tap water were performed: an in vitro pH titration study and an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) in vitro cytotoxicity cell viability assay. For pH normalization, 17 times more tap water than Diphoterine was required. In the cytotoxicity test, two-thirds of the cells remained viable after Diphoterine washing, compared with only one-third after tap water washing (p < .001). Diphoterine washing is a promising TMAH decontamination method.
Fosse
Céline
C
Laboratoire Prevor, Valmondois, France.
Mathieu
Laurence
L
Hall
Alan H
AH
Bocchietto
Elena
E
Burgher
François
F
Fischbach
Michel
M
Maibach
Howard I
HI
D002057:Burns, Chemical / Q000188:drug therapy* / Q000473:pathology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D004817:Epidermis / Q000473:pathology; D006801:Humans; D016273:Occupational Exposure / Q000009:adverse effects*; D009883:Ophthalmic Solutions / Q000627:therapeutic use*; D009930:Organic Chemicals / Q000627:therapeutic use; D000644:Quaternary Ammonium Compounds / Q000633:toxicity*; D012867:Skin / Q000473:pathology; D012871:Skin Diseases / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D013778:Tetrazolium Salts; D007507:Therapeutic Irrigation; D013844:Thiazoles; D014867:Water
D004396:Coloring Agents; D009883:Ophthalmic Solutions; D009930:Organic Chemicals; D000644:Quaternary Ammonium Compounds; D013778:Tetrazolium Salts; D013844:Thiazoles; C472007:diphoterine; D014867:Water; C022616:thiazolyl blue; C027917:tetramethylammonium