Alternative splicing of interleukin-7 (IL-7) and interleukin-7 receptor alpha (IL-7Ralpha) in peripheral blood from patients with multiple sclerosis (MS).
IL-7 and IL-7Ralpha (IL-7R) form a non-redundant ligand receptor system which plays a crucial role in human T cell immunity. Both IL-7 and IL-7R are multi-exonal genes and exhibit alternative splicing. We measured the relative distribution of IL-7 and IL-7R spliced mRNA from patients with MS and healthy individuals and observed extensive alternative splicing of both genes with marked differences in proportional transcript expression levels. We report here for the first time that the IL-7 transcript, lacking exon 4, and not the full length IL-7 represents the dominant IL-7 RNA transcript in human PBMCs and a novel IL-7R splice variant lacking exons 5, 6 and 7.
Department of Microbiology, Tumor and Cell Biology (MTC), Nobels Väg 18, Karolinska Institutet, SE-17182 Stockholm, Sweden.
Lalit
L
Rane
Nalini
N
Vudattu
Kasia
K
Bourcier
Eva
E
Graniar
Jan
J
Hillert
Vicki
V
Seyfert
M J
MJ
Maeurer
D017398:Alternative Splicing / Q000235:genetics*; D001483:Base Sequence / Q000235:genetics; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D004252:DNA Mutational Analysis; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D005820:Genetic Testing; D006801:Humans; D015851:Interleukin-7 / Q000235:genetics*; D009103:Multiple Sclerosis / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics*; D020395:Receptors, Interleukin-7 / Q000235:genetics*; D012680:Sensitivity and Specificity
D015415:Biomarkers; D005819:Genetic Markers; D015851:Interleukin-7; D012333:RNA, Messenger; D020395:Receptors, Interleukin-7; C415350:interleukin-7 receptor, alpha chain