Regeneration of intervertebral disc by the intradiscal application of cross-linked hyaluronate hydrogel and cross-linked chondroitin sulfate hydrogel in a rabbit model of intervertebral disc injury.
The degeneration of intervertebral disc (IVD) is a major cause of low back pain. However, there is no satisfactory preventive treatment for degenerative disc disease (DDD). In this study, we examined the effects of a novel cross-linked hyaluronate hydrogel and cross-linked chondroitin sulfate (CS) hydrogel on a rabbit model of IVD injury. We injected 300 microl of phosphate buffer saline, 1% sodium hyaluronate, cross-linked hyaluronate hydrogel, or cross-linked CS hydrogel into the injured IVDs. One, three or six months after treatment, the whole spinal columns were dissected and magnetic resonance (MR) images of the IVDs were examined. It was noted that the IVD, which was injected with cross-linked hyaluronate hydrogel or cross-linked CS hydrogel mostly retained the normal signal intensity of the MR images. These IVDs exhibited a higher degree of staining with safranin-O than the control discs or 1% sodium hyaluronate-injected discs, suggesting that the intradiscal application of cross-linked hyaluronate hydrogel or cross-linked CS hydrogel probably inhibits the degenerative cascade of the DDD. The intradiscal administration of these drugs is safe, easy and costs less. In the near future, these intradiscal injections may become the standard therapy for the treatment of DDD instead of the spine surgeries.
Department of Orthopaedic Surgery, Nagoya University School of Medicine, Showa-ku, Nagoya, Japan. nakashima@hello.email.ne.jp
Shojiro
S
Nakashima
Yukihiro
Y
Matsuyama
Katsuya
K
Takahashi
Tomoya
T
Satoh
Hiroshi
H
Koie
Kiichi
K
Kanayama
Taichi
T
Tsuji
Koji
K
Maruyama
Shiro
S
Imagama
Yoshihito
Y
Sakai
Naoki
N
Ishiguro
D000464:Alginates / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000627:therapeutic use*; D003432:Cross-Linking Reagents; D004195:Disease Models, Animal; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000627:therapeutic use*; D007403:Intervertebral Disc / Q000187:drug effects / Q000254:growth & development* / Q000473:pathology; D007405:Intervertebral Disc Displacement / Q000188:drug therapy* / Q000473:pathology*; D011817:Rabbits; D016896:Treatment Outcome
D000464:Alginates; D003432:Cross-Linking Reagents; C118495:hyaluronate alginate; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates