BIOMED-2 protocols to detect clonal immunoglobulin and T-cell receptor gene rearrangements in B- and T-cell lymphomas in southern Taiwan.
Monoclonal expansions of immunoglobulin (Ig) and T-cell receptor (TCR) genes are, respectively, important markers for B- and T-cell malignancies. We used BIOMED-2 protocols to detect clonality of these genes in B- (BCL) and T-cell lymphoma (TCL) in southern Taiwan. Heteroduplex analysis was used after PCR reactions. Ig/TCR clonality rates were 95% (22 in 23 cases) for BCL and 76% (19 in 25 cases) for TCL. These results reveal overall satisfactory detection rates for both BCL and TCL. None of the four natural killer (NK)/T-cell lymphomas detected showed TCR gene clonality, which suggested that BIOMED-2 protocols distinguished the NK/T-cell lymphoma from TCL. In addition, three of the five tested precursor T-cell lymphoblastic lymphomas showed no TCR gene clonality, probably because the immature T-cells in this type of TCL had not undergone TCR gene rearrangements. We conclude that BIOMED-2 protocols are suitable for detecting Ig and TCR clonalities in B- and T-cell malignancies in southern Taiwan.
Laboratory of Molecular Diagnostics, Department of Pathology, National Cheng Kung University Hospital, Tainan 70101, Taiwan.
Yi-Lin
YL
Chen
Ih-Jen
IJ
Su
Hsieh-Yin
HY
Cheng
Kung-Chao
KC
Chang
Cheng-Chan
CC
Lu
Nan-Haw
NH
Chow
Chung-Liang
CL
Ho
Wenya
W
Huang
D045744:Cell Line, Tumor; D002985:Clinical Protocols; D002999:Clone Cells / Q000276:immunology* / Q000378:metabolism; D004252:DNA Mutational Analysis / Q000379:methods; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics; D019672:Genes, T-Cell Receptor / Q000235:genetics; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis / Q000235:genetics; D016393:Lymphoma, B-Cell / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D016399:Lymphoma, T-Cell / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D013624:Taiwan
D007136:Immunoglobulins