Non-crystallographic symmetry of crystal of neutral phospholipase A(2) fromAgkistrodon halys Pallas.
Agkistrodotoxin, a neutral phospholipase A(2) with high presynaptic neurotoxicity from the venom ofAgkistrodon halys Pallas, has been crystallized by hanging drop vapor diffusion method. The crystal belongs to P2(1) space group with the cell dimensionsa = 10.836 nm,b = 8.486nm,c = 7.082nm, beta = 109.87 degrees , showing C2 pseudo-symmetry. Diffraction data to 0.26 nm resolution have been collected on a Siemens X-200B area detector. C2 pseudo symmetry suggests that there exists a non-crystallographic two-fold axis parallel to crystallographicb axis. Selfrotation function calculation with different integrated radius and resolution ranges using the program POLARRFN yields four stable high peaks corresponding to three more non-crystallographic two-fold axis and one special non-crystallographic symmetry. The molecules in the asymmetric unit are suggested to be arranged in a manner ofdimer of dimers" by inference.
Chinese Academy of Sciences, State Key Laboratory of Biomacromolecules. Institute of Biophysics, 100101, Beijing, China.
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