Proteome of platelets in patients with coronary artery disease.
OBJECTIVE
This study aimed at investigating the protein patterns of platelets from patients with stable or acute coronary atherosclerosis (CAD), in which platelets play a key role.


METHODS
A proteomic approach was adopted to investigate specific protein patterns in platelets of patients with non-ST elevation acute coronary syndrome, stable angina, or of subjects with no history of CAD.


RESULTS
Six differentially expressed proteins were identified: two involved in energy metabolism (2-oxoglutarate dehydrogenase [OGDH], and lactate dehydrogenase [LDH]); three were associated with cytoskeleton-based processes (gamma-actin, coronin 1B, and pleckstrin); and one involved in protein degradation (proteasome subunit type 8). Expression levels of OGDH and a cleaved form of gamma-actin were significantly higher in the platelets of patients than in controls, whereas that of LDH was higher only in the platelets of patients with acute coronary disease. The increases in protein expression of OGDH and LDH are paralleled by changes in their functional activities. Coronin and proteasome subunit type 8 were less expressed in the platelets of patients, as were the basic isoforms of pleckstrin.


CONCLUSIONS
The platelet proteome is altered in CAD patients with stable or acute coronary syndrome possibly because of the ongoing atherosclerotic process. The identified protein changes not previously connected with CAD were an increase in the energy metabolism enzymes and alterations in the proteins associated with cytoskeleton-based processes, both of which indicate platelet activation.
Centro Cardiologico Monzino, I.R.C.C.S, Milan, Italy. cristina.banfi@unimi.it
Cristina
C
Banfi
Maura
M
Brioschi
Giancarlo
G
Marenzi
Monica
M
De Metrio
Marina
M
Camera
Luciana
L
Mussoni
Elena
E
Tremoli
D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D054058:Acute Coronary Syndrome / Q000097:blood* / Q000235:genetics; D000368:Aged; D000595:Amino Acid Sequence; D000787:Angina Pectoris / Q000097:blood* / Q000235:genetics; D001792:Blood Platelets / Q000378:metabolism*; D001798:Blood Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D007655:Ketoglutarate Dehydrogenase Complex / Q000096:biosynthesis / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008840:Microfilament Proteins / Q000096:biosynthesis / Q000235:genetics; D008875:Middle Aged; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D046988:Proteasome Endopeptidase Complex / Q000096:biosynthesis / Q000235:genetics; D011493:Protein Kinase C / Q000378:metabolism; D020543:Proteome
D000199:Actins; D001798:Blood Proteins; D008840:Microfilament Proteins; C501479:PSMD8 protein, human; D010750:Phosphoproteins; D020543:Proteome; C039077:platelet protein P47; C072061:coronin proteins; D007770:L-Lactate Dehydrogenase; D007655:Ketoglutarate Dehydrogenase Complex; D011493:Protein Kinase C; D046988:Proteasome Endopeptidase Complex