Proteome analysis for rat saliva.
Proteome analysis is a popular method to discover biomarkers for the prevention and diagnosis of diseases. Since saliva is a non-invasively available body fluid, gathering of saliva causes minimal harm to patients. Therefore, detection of proteins for the prevention and diagnosis from the saliva sample may be the preferred method, especially for children and elderly people. However, the abundance of salivary proteins and contaminant proteins from food and mouth bacteria obscure identification of proteins present in the saliva at low concentrations. To address this problem, we developed a shotgun proteomic method using two-dimensional nano-flow LC tandem mass spectrometry. We report here that our method is able to detect proteins quantitatively even in small sample volumes of saliva.
Division of Physiology, Department of Biosciences, Kyushu Dental College, Kitakyushu, Japan.
Kiyotoshi
K
Inenaga
Naoyuki
N
Yamada
Reiko
R
Yuji
Misako
M
Kawai
Hisayuki
H
Uneyama
Kentaro
K
Ono
Ei-ichiro
E
Suzuki
Kunio
K
Torii
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods
D012471:Salivary Proteins and Peptides