Analysis of chromosomal structural polymorphisms in the St, P, and Y genomes of Triticeae (Poaceae).
The St, P, and Y genomes are three important genomes in the tribe Triticeae, which includes many perennial species. To study polymorphisms within the chromosomes of the St, P, and Y genomes, a GISH-FISH method was developed that allowed them to be clearly distinguished. The karyotypes of five individuals from population Z1925 of Kengyilia grandiglumis (Keng) J.L. Yang et al. (2n = 6x = 42, StStPPYY) were analyzed. The results showed that there were structural polymorphisms in all of the chromosomes from the three individual genomes. The polymorphisms were found mainly in the terminal regions of chromosomes and infrequently near the centromeric region. Of all the chromosomes, 1P, 1St, 1Y, 2Y, 3St, and 3Y showed the most polymorphisms. The polymorphisms within the individual chromosomes suggested that more extensive and scientific conclusions regarding the origin and evolution of genomes in wild species of Triticeae would be achieved by studying a population as a sampling and analysis unit.
Wang
Qiuxia
Q
The National Key Facilities for Crop Gene Resources and Genetic Improvement, NFCRI, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.
Xiang
Jishan
J
Gao
Ainong
A
Yang
Xinming
X
Liu
Weihua
W
Li
Xiuquan
X
Li
Lihui
L
D032461:Chromosomes, Plant / Q000235:genetics*; D014644:Genetic Variation; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D006109:Poaceae / Q000235:genetics*; D011110:Polymorphism, Genetic; D013045:Species Specificity