An introduction to mitochondrial informatics.
In this chapter, we review the public resources available for human mitochondrial DNA and protein related bioinformatics, with a special focus on mitochondrial single nucleotide polymorphisms (mtSNPs). We also review our own freeware tool V-MitoSNP, giving an overview of its implementation and program workflow. Apart from these, we review several protocols for the graphic input of genes, keywords, gene searching by sequence, mtSNP searching by sequence, restriction enzyme mining, primer design, and virtual electrophoresis for PCR-RFLP genotyping. Some databases with similar function are integrated and compared.
Chang
Hsueh-Wei
HW
Department of Biomedical Science and Environmental Biology, Center of Excellence for Environmental Medicine, Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan.
Chuang
Li-Yeh
LY
Cheng
Yu-Huei
YH
Gu
De-Leung
DL
Huang
Hurng-Wern
HW
Yang
Cheng-Hong
CH
D019295:Computational Biology / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics; D054629:Genome, Mitochondrial; D006801:Humans; D008928:Mitochondria / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
D004272:DNA, Mitochondrial