NGF-potentiating vibsane-type diterpenoids from Viburnum sieboldii.
Six new vibsane-type diterpenoids, named neovibsanin O (1), neovibsanin M (2), neovibsanin L (3), (8Z)-neovibsanin M (4), 15-O-methylvibsanin H (5), and 5-epi-15-O-methylvibsanin H (6), were isolated from the leaves of Viburnum sieboldii by bioassay-guided fractionation using NGF-differentiated PC12 cells. The structures of 1-6 were established by analyzing their spectroscopic data and comparing their NMR data with those of previously reported vibsane-type diterpenoids. Compounds 3 and 4, and the known vibsane-type diterpenoids neovibsanins A (7) and B (8) significantly enhanced the neurite outgrowth of NGF-mediated PC12 cells at concentrations ranging from 20 to 40 microM.
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8514, Japan.
Miwa
M
Kubo
Yoshiko
Y
Kishimoto
Kenichi
K
Harada
Hideaki
H
Hioki
Yoshiyasu
Y
Fukuyama
D000818:Animals; D001681:Biological Assay; D002454:Cell Differentiation / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D004357:Drug Synergism; D009682:Magnetic Resonance Spectroscopy; D020932:Nerve Growth Factor / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D016339:Spectrometry, Mass, Fast Atom Bombardment; D027925:Viburnum / Q000737:chemistry*
D004224:Diterpenes; D020932:Nerve Growth Factor