Mitochondrial fatty acid synthesis and respiration.
Recent studies have revealed that mitochondria are able to synthesize fatty acids in a malonyl-CoA/acyl carrier protein (ACP)-dependent manner. This pathway resembles bacterial fatty acid synthesis (FAS) type II, which uses discrete, nuclearly encoded proteins. Experimental evidence, obtained mainly through using yeast as a model system, indicates that this pathway is essential for mitochondrial respiratory function. Curiously, the deficiency in mitochondrial FAS cannot be complemented by inclusion of fatty acids in the culture medium or by products of the cytosolic FAS complex. Defects in mitochondrial FAS in yeast result in the inability to grow on nonfermentable carbon sources, the loss of mitochondrial cytochromes a/a3 and b, mitochondrial RNA processing defects, and loss of cellular lipoic acid. Eukaryotic FAS II generates octanoyl-ACP, a substrate for mitochondrial lipoic acid synthase. Endogenous lipoic acid synthesis challenges the hypothesis that lipoic acid can be provided as an exogenously supplied vitamin. Purified eukaryotic FAS II enzymes are catalytically active in vitro using substrates with an acyl chain length of up to 16 carbon atoms. However, with the exception of 3-hydroxymyristoyl-ACP, a component of respiratory complex I in higher eukaryotes, the fate of long-chain fatty acids synthesized by the mitochondrial FAS pathway remains an enigma. The linkage of FAS II genes to published animal models for human disease supports the hypothesis that mitochondrial FAS dysfunction leads to the development of disorders in mammals.
Hiltunen
J Kalervo
JK
Department of Biochemistry and Biocenter Oulu, University of Oulu, PO Box 3000, FI-90014 Oulu, Finland. kalervo.hiltunen@oulu.fi
Autio
Kaija J
KJ
Schonauer
Melissa S
MS
Kursu
V A Samuli
VA
Dieckmann
Carol L
CL
Kastaniotis
Alexander J
AJ
D000818:Animals; D019069:Cell Respiration; D054889:Fatty Acid Synthase, Type II / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D006801:Humans; D054878:Lipoylation; D008928:Mitochondria / Q000378:metabolism*; D008954:Models, Biological; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D000077278:RNA, Mitochondrial; D008063:Thioctic Acid / Q000096:biosynthesis
D005227:Fatty Acids; D000077278:RNA, Mitochondrial; D012313:RNA; D008063:Thioctic Acid; D054889:Fatty Acid Synthase, Type II