[Relationship between the inhibition of NF-kappaB and insulin resistance in uterrus of rats with gestational diabetes mellitus].
OBJECTIVE
To observe the expression of nuclear factor-kappaB (NF-kappaB) and glucose transporter-4 (GLUT-4) in uteru of rats with gestational diabetes mellitus (GDM) and to explore the relationship between inhibition of NF-kappaB and insulin resistance.


METHODS
Thirty Sprague-Dawley rats were randomly divided into 3 groups: normal pregnant control (NC) group, GDM group and PDTC group, 10 rats each group. GDM rat model was established by intraperitoneal (ip) injection of streptozotocin (STZ). PDTC (40 mg/kg) was ip injected in PDTC group.The rats were sacrificed on the 20-day of pregnancy before delivery. The uterus were taken to observe the pathological changes. The expressions of GLUT-4 and NF-kappaB in uteru tissue were detected by immunohistochemical staining and Western blot.


RESULTS
The renal NF-kappaB activity in GDM group was higher significantly than that in NC group (P<0.01); and NF-kappaB activity in PDTC group was lower than that in GDM group (P<0.01). The expression of GLUT-4 in GDM group was significantly lower than that in NC group (P<0.01). However, compared with GDM group, the expression of GLUT-4 in PDTC group was increased (P<0.01).


CONCLUSIONS
The inhibition of NF-kappaB activity can increase the expression of GLUT-4 in uterus of GDM rats, indicating that the occurrence of GDM in rats with uteru insulin resistance may be related with the intervention of NF-kappaB activity on the down regulation of the expression of GLUT-4.
Department of Gynecology and Obstetrics, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Bo
B
Wei
Na
N
Ta
Jia
J
Li
Jian-fang
JF
Zhang
Bi-liang
BL
Chen
D000818:Animals; D016640:Diabetes, Gestational / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007333:Insulin Resistance; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000378:metabolism*
D051275:Glucose Transporter Type 4; D016328:NF-kappa B; C495693:Slc2a4 protein, mouse