Differential effects of ethanol and other inducers of drug metabolism on the two forms of hamster liver microsomal aniline hydroxylase.
The aniline hydroxylase activity of microsomes isolated from hamster liver can be differentiated kinetically into high affinity (low K(m), form I) and low affinity (high K(m), form II) forms. Microsomes isolated from uninduced animals contain slightly more form I activity. The activity of the low affinity form (form II) is preferentially enhanced by Aroclor or 3-methylcholanthrene treatment, while phenobarbital treatment increases the activity of both forms. Chronic ethanol consumption results in enhancement of only the high affinity form (form I).
Division of Molecular Biology and Pharmacology, Naylor Dana Institute for Disease Prevention, American Health Foundation, Valhalla, NY 10595, USA.
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D000815:Aniline Hydroxylase / Q000096:biosynthesis*; D000818:Animals; D020111:Chlorodiphenyl (54% Chlorine) / Q000494:pharmacology*; D006224:Cricetinae; D004790:Enzyme Induction / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D008748:Methylcholanthrene / Q000494:pharmacology*; D008861:Microsomes / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology*; D011897:Random Allocation
D007527:Isoenzymes; D020111:Chlorodiphenyl (54% Chlorine); D000431:Ethanol; D008748:Methylcholanthrene; D000815:Aniline Hydroxylase; D010634:Phenobarbital