Cytometric catheter for neurosurgical applications.
Implantation of neural progenitor cells into the central nervous system has attracted strong interest for treatment of a variety of pathologies. The replacement of dopamine-producing neural cells in the brain appears promising for the treatment of patients affected by Parkinson's disease. Previous studies of cell replacement strategies have shown that less than 10% of implanted cells were viable 24-48 hours following implantation. We present the design of an instrumented cell-delivery catheter that has been developed to facilitate the quantification of the cells delivered and determination of viability. The catheter uses a fibre optic probe to perform fluorescence-based cytometric measurements on cells exiting the port at the catheter tip. Results of fluorescence testing data are presented and show that the device can characterize the quantity of cell densities ranging from 60 000 to 600 000 cells ml(-1) with a coefficient of determination of 0.93 (p < 0.05, n = 6).
Evans
B M
BM
Biomedical Sciences and Engineering Center, Oak Ridge National Laboratory, PO Box 2008, Oak Ridge, TN 37831-6006, USA. evansbmiii@ornl.gov
Allison
S W
SW
Fillmore
H L
HL
Broaddus
W C
WC
Dyer
R L
RL
Gillies
G T
GT
D000818:Animals; D002404:Catheterization / Q000379:methods*; D064987:Cell- and Tissue-Based Therapy / Q000295:instrumentation; D004867:Equipment Design; D006801:Humans; D019635:Neurosurgical Procedures / Q000295:instrumentation*