[Expression of c-jun during the incised wound healing in mice skin].
OBJECTIVE To investigate the time-dependent expression of c-jun during the healing of incised wound in mice skin.


METHODS
The expression of c-jun in different stages after the incised wound were detected by immunohistochemistry and Western blot.


RESULTS
There was a low level expression of c-jun in normal mice skin. Expression of c-jun was mainly detected in neutrophils from 3 h to 12h after injury. The c-jun positive cells were almost mononuclear cells (MNCs) and fibroblasts between 1 d and 5 d after injury. The c-jun positive cells were mostly fibroblasts between 7 d and 14 d after injury. The ratio of the c-jun positive cells increased in the wound specimens from 3 h to 12 h, peaked at 12 h, declined partially from 1 d to 5 d, and reached the peak secondly at 7 d, then decreased from 10 d to 14 d. The expression of c-jun was observed throughout the wound healing stages by Western blot with two peaks occurring at 12 h and 7 d after injury.


CONCLUSIONS
The c-jun may play a potential role in inducing apoptosis of neutrophils, MNCs and fibroblasts during skin wound healing, and it may be used as the marker for wound age determination.
School of Forensic Medicine, China Medical University, Shenyang 110001, China. ymdoctor@163.com
Me
M
Yang
Da-Wei
DW
Guan
Chang-Yan
CY
Xiong
Zi-Hui
ZH
Cheng
Tian-Shui
TS
Yu
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D004195:Disease Models, Animal; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D011897:Random Allocation; D012867:Skin / Q000293:injuries* / Q000378:metabolism*; D013997:Time Factors; D014945:Wound Healing; D014947:Wounds and Injuries / Q000378:metabolism
D016755:Proto-Oncogene Proteins c-jun