Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples.
We report a sensitive method for selective detection of target metabolites from the central metabolic pathway by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), in which the MS signal is enhanced by up to three orders of magnitude in the course of in situ enzymatic amplification.
Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland.
P L
PL
Urban
A
A
Amantonico
S R
SR
Fagerer
P
P
Gehrig
R
R
Zenobi
D000105:Acetyl Coenzyme A / Q000737:chemistry; D000244:Adenosine Diphosphate / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D003065:Coenzyme A / Q000737:chemistry; D004798:Enzymes / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004798:Enzymes; D000244:Adenosine Diphosphate; D000105:Acetyl Coenzyme A; D000255:Adenosine Triphosphate; D003065:Coenzyme A