Characterization of antibiotic resistance in Vibrio spp. isolated from farmed marine shrimps (Penaeus monodon).
A total of 83 Vibrio isolates from farmed marine shrimps (Penaeus monodon) were tested for the presence of class 1, 2 and 3 integrons, SXT constin and tetracycline resistance-encoding genes. Mutations in the quinolone resistance-determining regions (QRDRs) of the gyrA and parC genes were determined in fluoroquinolone-resistant Vibrio strains (n=17). Five isolates were found to carry class 1 integrons, of which only one contained the partial rumA gene in the variable region. All the Vibrio strains were devoid of class 2 and 3 integrons. Seven isolates harbored SXT constin. None of the Vibrio isolates were positive to the tet(K), tet(L), tet(M), tet(O) and tet(S) genes. Ten fluoroquinolone-resistant Vibrio strains carried a point mutation G-248-T in the gyrA QRDR, leading to a Ser-83-Ile substitution in GyrA, but none of these strains had mutations in the QRDR of the parC gene.
Kitiyodom
Sirikorn
S
Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Khemtong
Sirintip
S
Wongtavatchai
Janenuj
J
Chuanchuen
Rungtip
R
D000818:Animals; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D032023:Integrons; D008826:Microbial Sensitivity Tests; D009154:Mutation; D033561:Penaeidae / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000187:drug effects* / Q000235:genetics*
D004269:DNA, Bacterial; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase