A novel 18-bp deletion mutation of the AMPD1 gene affects carcass traits in Qinchuan cattle.
The objectives of the present study were focused on detecting deletion mutation in bovine AMPD1 gene, and analyzing its effect on body measurement and carcass traits in Qinchuan cattle by using DNA sequencing and agarose electrophoresis methods. The 198-bp PCR products of AMPD1 gene exhibited three genotypes and two alleles were revealed: A and B. The frequencies of genotype AA/AB/BB in Qinchuan populations was 0.7163, 0.2233 and 0.0605. The χ(2)-test analysis demonstrated that the breed was not in agreement with Hardy-Weinberg equilibrium (P < 0.05). The association of the 18-bp deletion mutation of AMPD1 gene with body measurement and carcass traits of Qinchuan cattle were analyzed. The cattle with AA genotype had slaughter weight and carcass weight than those with genotype AB (P < 0.01 or P < 0.05). These results suggest that the 18-bp deletion mutation may influence the carcass traits in Qinchuan cattle.
He
Hua
H
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, People's Republic of China. hehua686@yahoo.com.cn
Liu
Xiaolin
X
Gu
Yulan
Y
Liu
Yu
Y
D000659:AMP Deaminase / Q000235:genetics*; D000818:Animals; D020029:Base Pairing / Q000235:genetics*; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D002681:China; D056426:Genetic Loci / Q000235:genetics; D005838:Genotype; D007438:Introns / Q000235:genetics; D008460:Meat; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D019655:Quantitative Trait, Heritable; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000235:genetics*
D000659:AMP Deaminase