Characterization of a new defensin from cowpea (Vigna unguiculata (L.) Walp.).
Using Phaseoleae defensins available in databases, a putative defensin gene was isolated in cowpea (Vigna unguiculata (L.) Walp.) and cloned from genomic cowpea DNA. The putative mature defensin sequence displays the characteristic defensins residues arrangement, secondary and tertiary structures were predicted and splicing analysis was performed. Using RT-PCR, defensin expression and differences in response to biotic stimuli between infected and non infected plants were tested.
Department of Life Sciences, University of Trieste - Via Giorgieri, 1 - 34127 Trieste, Italy. padovan@burlo.trieste.it
Lara
L
Padovan
Ludovica
L
Segat
Alessandro
A
Tossi
Tercilio
T
Calsa
Akio Kido
AK
Ederson
Lucas
L
Brandao
Rafael L
RL
Guimarães
Valesca
V
Pandolfi
Maria Clara
MC
Pestana-Calsa
Luís Carlos
LC
Belarmino
Ana Maria
AM
Benko-Iseppon
Sergio
S
Crovella
D000595:Amino Acid Sequence; D001483:Base Sequence; D017798:Comovirus; D023082:Defensins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein / Q000379:methods*
D023082:Defensins; D010940:Plant Proteins