Identification and characterization of two phospholipid hydroperoxide glutathione peroxidase genes from Apis cerana cerana.
Phospholipid hydroperoxide glutathione peroxidase (PHGPX) plays a crucial role in maintaining the integrity of membrane by reducing hydroperoxides of phospholipids. Here, we report the identification and characterization of two genes, designated AccGtpx-1 and AccGtpx-2, encoding PHGPX proteins from the Chinese honeybees, Apis cerana cerana. Alignment analysis showed that AccGtpx-1 and AccGtpx-2 shared high similarity with other known PHGPXs, which show similar structure to thioredoxin. These single copy genes showed complex exon-intron structures. The mRNA of AccGtpx-1 was detected in larvae, pupae and adults and that AccGtpx-2 was only found in adult worker bees. Furthermore, the expression of AccGtpx-1 could be induced by H(2)O(2), ultraviolet (UV) light, heat shock (37 degrees C), HgCl(2), imidacloprid, cyhalothrin, pyriproxyfen and methomyl. In contrast, AccGtpx-2 expression could only be induced by UV. These results indicated for the first time that the AccGtpx-1 and AccGtpx-2 genes encoding A. cerana cerana PHGPXs are regulated differently in response to environmental stressors.
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, PR China.
Mian
M
Wang
Mingjiang
M
Kang
Xingqi
X
Guo
Baohua
B
Xu
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000201:enzymology* / Q000235:genetics*; D005979:Glutathione Peroxidase / Q000235:genetics*; D008969:Molecular Sequence Data; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods
D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase