Adipose tissue-derived stem cells rescue Purkinje neurons and alleviate inflammatory responses in Niemann-Pick disease type C mice.
Adult stem cells offer special therapeutic prospects because they can be isolated for autologous transplantation, expanded ex vivo, and differentiated into various cell types. We previously reported that bone marrow-derived mesenchymal stem cells improve neurological deficits in neurodegenerative disease animal models. However, the efficacy of adipose tissue-derived stem cells (ADSCs) transplantation in similar models remains unknown. Herein, we demonstrate that ADSCs, when transplanted into Niemann-Pick disease type C (NP-C) mouse cerebellum, elicit rescue of Purkinje neurons and restoration of motor coordination together with alleviation of inflammatory responses as verified by immunohistochemistry and real-time PCR using glial fibrillary acidic protein (GFAP), F4/80, IL-1 beta, IL-6, and TNF-alpha. Most importantly, ADSCs enhance electrically active Purkinje neurons with functional synaptic formation after transplantation in NP-C disease model mice. This report demonstrates for the first time that ADSCs can rescue imperiled Purkinje neurons and alleviate the inflammatory response in NP-C disease model mice, thereby signifying the therapeutic potential of ADSCs for neurodegenerative diseases.
Department of Physiology, Cell and Matrix Research Institute, BSEI, World Class University Program, School of Medicine, Kyungpook National University, Daegu, Korea. jsbae@knu.ac.kr
Jae-sung
JS
Bae
Janet E
JE
Carter
Hee Kyung
HK
Jin
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002470:Cell Survival; D002531:Cerebellum / Q000473:pathology / Q000503:physiopathology; D002784:Cholesterol / Q000378:metabolism; D004195:Disease Models, Animal; D055724:Electrophysiological Phenomena; D005434:Flow Cytometry; D007249:Inflammation / Q000473:pathology / Q000517:prevention & control*; D008279:Magnetic Resonance Imaging; D051379:Mice; D008807:Mice, Inbred BALB C; D009457:Neuroglia / Q000378:metabolism / Q000473:pathology; D052556:Niemann-Pick Disease, Type C / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D011689:Purkinje Cells / Q000378:metabolism / Q000473:pathology*; D013109:Sphingomyelins / Q000378:metabolism; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*
D013109:Sphingomyelins; D002784:Cholesterol