RpoE2 of Sinorhizobium meliloti is necessary for trehalose synthesis and growth in hyperosmotic media.
Adaptation to osmotic stress can be achieved by the accumulation of compatible solutes that aid in turgor maintenance and macromolecule stabilization. The genetic regulation of solute accumulation is poorly understood, and has been described well at the molecular level only in enterobacteria. In this study, we show the importance of the alternative sigma factor RpoE2 in Sinorhizobium meliloti osmoadaptation. Construction and characterization of an S. meliloti rpoE2 mutant revealed compromised growth in hyperosmotic media. This defect was due to the lack of trehalose, a minor carbohydrate osmolyte normally produced in the initial stages of growth and in stationary phase. We demonstrate here that all three trehalose synthesis pathways are RpoE2 dependent, but only the OtsA pathway is important for osmoinducible trehalose synthesis. Furthermore, we confirm that the absence of RpoE2-dependent induction of otsA is the cause of the osmotic phenotype of the rpoE2 mutant. In conclusion, we have highlighted that, despite its low level, trehalose is a crucial compatible solute in S. meliloti, and the OtsA pathway induced by RpoE2 is needed for its accumulation under hyperosmotic conditions.
Flechard
Maud
M
UMR CNRS 6026, DUALS, Université de Rennes I, Campus de Beaulieu, Av. du Général Leclerc, 35042 Rennes, France.
Fontenelle
Catherine
C
UMR CNRS 6026, DUALS, Université de Rennes I, Campus de Beaulieu, Av. du Général Leclerc, 35042 Rennes, France.
Blanco
Carlos
C
UMR CNRS 6026, DUALS, Université de Rennes I, Campus de Beaulieu, Av. du Général Leclerc, 35042 Rennes, France.
Goude
Renan
R
UMR CNRS 6026, DUALS, Université de Rennes I, Campus de Beaulieu, Av. du Général Leclerc, 35042 Rennes, France.
Ermel
Gwennola
G
UMR CNRS 6026, DUALS, Université de Rennes I, Campus de Beaulieu, Av. du Général Leclerc, 35042 Rennes, France.
Trautwetter
Annie
A
UMR CNRS 6026, DUALS, Université de Rennes I, Campus de Beaulieu, Av. du Général Leclerc, 35042 Rennes, France.
D000222:Adaptation, Physiological; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D009154:Mutation; D009995:Osmosis; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D014199:Trehalose / Q000096:biosynthesis*
D012808:Sigma Factor; D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase