Antimicrobial and immunomodulatory effect of clarithromycin on macrolide-resistant Mycoplasma pneumoniae.
Macrolide antibiotics are frequently administered to treat mycoplasmal pneumonia. However, macrolide-resistant Mycoplasma pneumoniae has recently been isolated from clinical specimens in Japan. Clarithromycin (CAM) is a 14-membered-ring macrolide that has host immunomodulatory activity. Here, we established a gnotobiotic mouse model that was monoassociated with macrolide-resistant M. pneumoniae, and pathologically and microbiologically analysed the effects of antibiotics against mycoplasmal pneumonia. We also examined the immunomodulatory activities of macrolide antibiotics in human lung carcinoma A549 cells in vitro and in a specific-pathogen-free (SPF) mouse model of pneumonia induced by M. pneumoniae antigen in vivo. CAM anti-mycoplasma antibiotics decreased the number of macrolide-sensitive and -resistant M. pneumoniae in the lungs of gnotobiotic mice. Thus, in SPF mice, CAM modulated pulmonary inflammation induced by M. pneumoniae antigens.
Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan.
Satoshi
S
Kurata
Department of Immunology, Faculty of Health Sciences, Kyorin University, Tokyo, Japan.
Haruhiko
H
Taguchi
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan.
Tsuguo
T
Sasaki
Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
Yasunori
Y
Fujioka
Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan.
Shigeru
S
Kamiya
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D017291:Clarithromycin / Q000494:pharmacology*; D004195:Disease Models, Animal; D024881:Drug Resistance, Bacterial; D004847:Epithelial Cells / Q000187:drug effects; D005260:Female; D005856:Germ-Free Life; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D007564:Japan; D018942:Macrolides / Q000494:pharmacology*; D051379:Mice; D009177:Mycoplasma pneumoniae / Q000187:drug effects*; D011019:Pneumonia, Mycoplasma / Q000188:drug therapy / Q000382:microbiology
D000900:Anti-Bacterial Agents; D007155:Immunologic Factors; D018942:Macrolides; D017291:Clarithromycin