Characterization and expression of a novel holotype insecticidal crystal protein gene from native Bacillus thuringiensis BM59-2.
We characterized a novel holotype cry gene (cry52Ba1) harbored in a Bacillus thuringiensis isolate BM59-2 native to China that expresses a spherical crystal protein of about 80 kDa. In this study, the full length of the cry52Ba1 gene was cloned from this strain. Sequence analysis of the gene was also performed. Furthermore, cry52Ba1 was expressed in Escherichia coli BL21(DE)pLysS, and the resulting insecticidal activity showed that the Cry52Ba1 protein exhibited high larvicidal activity against Aedes aegypti (Diptera), with a lethal concentration 50 of 1.526 microg/mL (95% confidence: 0.740-3.499 microg/mL).
Rice Research Institute, Sichuan Agricultural University, Wenjiang, P.R. China.
Aiping
A
Zheng
Jun
J
Zhu
Lingxia
L
Wang
Shuangcheng
S
Li
Qiming
Q
Deng
Shiquan
S
Wang
Furong
F
Tan
Xiumei
X
Yu
Peng
P
Guan
Hongxia
H
Liang
Ping
P
Li
D000330:Aedes / Q000187:drug effects; D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000302:isolation & purification / Q000472:pathogenicity*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity*; D002681:China; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity*; D006801:Humans; D007814:Larva / Q000187:drug effects; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis