RDS in cones does not interact with the beta subunit of the cyclic nucleotide gated channel.
Retinal degeneration slow (RDS) is a photoreceptor specific tetraspanin membrane protein. It is expressed in the rim region of rod outer segment (OS) discs and cone OS lamellae. Mutations in RDS cause both rod and cone-dominant retinal degenerations. We have recently shown that RDS functions differently in rods vs. cones, and have used the cone-dominant nrl ( -/- ) and rod-dominant wild-type (WT) murine retinas to study these differences and help understand the mechanism of rod and cone OS biogenesis. We hypothesize that the differential role of RDS in rods vs. cones is in part related to differences in RDS binding partners. RDS has been shown to bind to the GARP portion of the beta subunit of the rod-cyclic nucleotide gated (CNG) channel. This interaction has been hypothesized to play a role in anchoring the disc rim to the rod plasma membrane. In this study we show that RDS does not interact with the cone CNG. Given that cone lamellae are not entirely encased in plasma membrane and therefore may have different anchoring requirements compared with rods, this observation may help explain some of the differential behavior of RDS in rods vs. cones.
Conley
Shannon M
SM
Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma, OK 73104, USA.
Ding
Xi-Qin
XQ
Naash
Muna I
MI
D000818:Animals; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064531:Peripherins; D011485:Protein Binding; D055503:Protein Multimerization; D021122:Protein Subunits / Q000378:metabolism*; D017949:Retinal Cone Photoreceptor Cells / Q000166:cytology / Q000378:metabolism*; D017948:Retinal Rod Photoreceptor Cells / Q000166:cytology / Q000378:metabolism
C532622:CNGB3 protein, mouse; C518237:Cnga3 protein, mouse; D054815:Cyclic Nucleotide-Gated Cation Channels; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D064531:Peripherins; D021122:Protein Subunits; C578996:Prph2 protein, mouse