Musculoskeletal deformities secondary to neurotomy of the superior trunk of the brachial plexus in neonatal mice.
The developmental course of musculoskeletal deformities in neonatal brachial plexus palsy (NBPP) has not been studied extensively. The goals of this study were to: (1) evaluate a new animal model of NBPP, (2) characterize the development of musculoskeletal abnormalities in paralyzed shoulders, and (3) investigate the expression of myogenic and adipogenic genes in paralyzed rotator cuff muscles. Neonatal mice were divided into neurotomy and sham groups. The neurotomy group underwent surgical transection of the superior trunk of the brachial plexus within 24 h of birth. The sham group underwent the same surgical exposure, but the brachial plexus was left intact. Musculoskeletal deformities were evaluated with radiological and histological assays at 2, 4, 8, 12, and 30 weeks after birth. The supraspinatus muscles of a separate group of mice were used to examine expression of myogenic and adipogenic genes at 8 weeks. The neurotomized forelimbs developed deformities similar to those seen in human NBPP. The deformities progressed with age. The denervated supraspinatus muscles showed intramuscular fat accumulation and upregulation of both myogenic and adipogenic genes compared to the normal. The current study presents a useful animal model for future research examining musculoskeletal changes secondary to neonatal nerve injury.
Department of Orthopaedic Surgery, Washington University, 660 South Euclid, Campus Box 8233, St. Louis, Missouri 63110, USA.
H Mike
HM
Kim
Leesa M
LM
Galatz
Rosalina
R
Das
Nikunj
N
Patel
Stavros
S
Thomopoulos
D000818:Animals; D000831:Animals, Newborn; D001917:Brachial Plexus / Q000601:surgery*; D020516:Brachial Plexus Neuropathies / Q000150:complications* / Q000209:etiology; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000378:metabolism / Q000503:physiopathology; D009139:Musculoskeletal Abnormalities / Q000209:etiology*; D018006:Myogenic Regulatory Factors / Q000378:metabolism; D016059:Range of Motion, Articular / Q000502:physiology; D017006:Rotator Cuff / Q000503:physiopathology
D018006:Myogenic Regulatory Factors