Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization.
BACKGROUND
Lactococcus garvieae is a bacterial pathogen that affects different animal species in addition to humans. Despite the widespread distribution and emerging clinical significance of L. garvieae in both veterinary and human medicine, there is almost a complete lack of knowledge about the genetic content of this microorganism. In the present study, the genomic content of L. garvieae CECT 4531 was analysed using bioinformatics tools and microarray-based comparative genomic hybridization (CGH) experiments. Lactococcus lactis subsp. lactis IL1403 and Streptococcus pneumoniae TIGR4 were used as reference microorganisms.


RESULTS
The combination and integration of in silico analyses and in vitro CGH experiments, performed in comparison with the reference microorganisms, allowed establishment of an inter-species hybridization framework with a detection threshold based on a sequence similarity of >or= 70%. With this threshold value, 267 genes were identified as having an analogue in L. garvieae, most of which (n = 258) have been documented for the first time in this pathogen. Most of the genes are related to ribosomal, sugar metabolism or energy conversion systems. Some of the identified genes, such as als and mycA, could be involved in the pathogenesis of L. garvieae infections.


CONCLUSIONS
In this study, we identified 267 genes that were potentially present in L. garvieae CECT 4531. Some of the identified genes could be involved in the pathogenesis of L. garvieae infections. These results provide the first insight into the genome content of L. garvieae.
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Mónica
M
Aguado-Urda
Guillermo H
GH
López-Campos
José F
JF
Fernández-Garayzábal
Fernando
F
Martín-Sánchez
Alicia
A
Gibello
Lucas
L
Domínguez
María M
MM
Blanco
D055028:Comparative Genomic Hybridization / Q000379:methods; D019295:Computational Biology / Q000379:methods; D003198:Computer Simulation; D004269:DNA, Bacterial / Q000737:chemistry; D030541:Databases, Genetic; D016680:Genome, Bacterial; D017045:Lactococcus / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D013045:Species Specificity; D013296:Streptococcus pneumoniae / Q000235:genetics
D004269:DNA, Bacterial