Antioxidant effect of functionalized alkyl-organotellurides: a study in vitro.
We evaluated the in vitro antioxidant effect of alkyl-organotellurides A-D on lipid peroxidation and protein carbonylation in rat liver homogenates. The thiol oxidase and thiol peroxidase-like activities of compounds were investigated. delta-Aminolevulinic acid dehydratase (delta-ALA-D) activity was determined in rat liver homogenates. Compounds A-D protected against lipid peroxidation induced by Fe(2+)/EDTA and sodium nitroprusside (SNP). According to the confidence limits of the IC(50) values of compounds A-D, the IC(50) values for organotellurides followed the order: C (0.30 microM) < or = B (0.40 microM) < D (0.68 microM) < A (2.90 microM), for Fe(2+)/EDTA, and B (0.21 microM) < or = C (0.33 microM) < or = D (0.43 microM) < A (1.21 microM) for SNP-induced lipid peroxidation. Compounds A-D reduced protein carbonyl content to control levels. The results demonstrated an inverse correlation between thiol oxidase and delta-ALA-D activities. This study supports an antioxidant effect of organotellurides A-D on rat liver.
Gay
Bibiana M
BM
Department of Chemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Luchese
Cristiane
C
Nogueira
Cristina W
CW
Wendler
P
P
Macedo
Alexandra
A
Dos Santos
Alcindo A
AA
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D009942:Organometallic Compounds / Q000737:chemistry*; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000378:metabolism; D000623:Porphobilinogen Synthase / Q000378:metabolism; D051381:Rats; D013329:Structure-Activity Relationship; D013691:Tellurium
D000975:Antioxidants; D009942:Organometallic Compounds; D050862:Oxidoreductases Acting on Sulfur Group Donors; C112161:thiol oxidase, 47 kDa; D000623:Porphobilinogen Synthase; D013691:Tellurium