Rapid polymerase chain reaction diagnosis of white-nose syndrome in bats.
A newly developed polymerase chain reaction (PCR)-based method to rapidly and specifically detect Geomyces destructans on the wings of infected bats from small quantities (1-2 mg) of tissue is described in the current study (methods for culturing and isolating G. destructans from bat skin are also described). The lower limits of detection for PCR were 5 fg of purified fungal DNA or 100 conidia per 2 mg of wing tissue. By using histology as the standard, the PCR had a diagnostic specificity of 100% and a diagnostic sensitivity of 96%, whereas the diagnostic sensitivity of culture techniques was only 54%. The accuracy and fast turnaround time of PCR provides field biologists with valuable information on infection status more rapidly than traditional methods, and the small amount of tissue required for the test would allow diagnosis of white-nose syndrome in live animals.
Lorch
Jeffrey M
JM
U.S. Geological Survey-National Wildlife Health Center, 6006 Schroeder Road, Madison, WI 53711, USA.
Gargas
Andrea
A
Meteyer
Carol Uphoff
CU
Berlowski-Zier
Brenda M
BM
Green
D Earl
DE
Shearn-Bochsler
Valerie
V
Thomas
Nancy J
NJ
Blehert
David S
DS
D000818:Animals; D001203:Ascomycota / Q000302:isolation & purification*; D002685:Chiroptera; D003881:Dermatomycoses / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D013997:Time Factors