Enzymatic degradation of hybrid iota-/nu-carrageenan by Alteromonas fortis iota-carrageenase.
Hybrid iota-/nu-carrageenan was water-extracted from Eucheuma denticulatum and incubated with Alteromonas fortis iota-carrageenase. The degradation products were then separated by anion-exchange chromatography. The three most abundant fractions of hybrid iota-/nu-carrageenan oligosaccharides were purified and their structures were analyzed by NMR. The smallest hybrid was an octasaccharide with a iota-iota-nu-iota structure. The second fraction was composed of two decasaccharides with iota-iota-iota-nu-iota and iota-[iota/nu]-iota-iota structures. The third fraction was a mixture of dodecasaccharides which contained at least a iota-iota-iota-iota-nu-iota oligosaccharide. The carbon and proton NMR spectra of the octasaccharides were completely assigned, thereby completely attributing the nu-carrabiose moiety for the first time.
Université Pierre et Marie Curie, Paris VI, CNRS, Marine Plants and Biomolecules, UMR 7139, Station Biologique, BP 74, F-29680 Roscoff Cedex, France.
Diane
D
Jouanneau
Patrick
P
Boulenguer
Jacques
J
Mazoyer
William
W
Helbert
D020591:Alteromonas / Q000201:enzymology*; D002351:Carrageenan / Q000737:chemistry* / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D000461:Rhodophyta / Q000737:chemistry
D002351:Carrageenan; D006026:Glycoside Hydrolases