Gold nanoparticle-based colorimetric assay of single-nucleotide polymorphism of triplex DNA.
Triplex DNA technology has been considered as an attractive antigen strategy for the treatment of genetic-based diseases. Assay of the formation of triplex is an important part in the development of triplex technology. In this paper, we present a novel method to assay triplex DNA. The strategy is based on the unspecific interaction between single-stranded triplex-forming oligonucleotide (TFO) and negatively charged gold nanoparticles (AuNPs). While triplex is formed, gold nanoparticles will aggregate without the protection of triplex-forming oligonucleotide under a certain concentration of salt. Consequently, the color of the gold nanoparticles will change from red to blue. The formation of triplex DNA and the discrimination of triplex-forming oligonucleotide candidates are thereby easily monitored by the color changes of gold nanoparticles. Also by precisely controlling the working salt concentration, we are allowed to assay single-nucleotide polymorphism of triplex-forming oligonucleotides. Mismatched variants and length variants of triplex-forming oligonucleotides with single-nucleotide or double-nucleotides differences can be well discriminated. This method presented here is simple, fast, and with considerable selectivity, so we expect it will be a promising candidate for the assay of triplex DNA and the screening of appropriate triplex-forming oligonucleotide.
Zhu
Xiaoli
X
Laboratory of Biosensing Technology, School of Life Science, Shanghai University, Shanghai 200444, PR China.
Liu
Yuexing
Y
Yang
Jinghua
J
Liang
Zhiqiang
Z
Li
Genxi
G
D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D006046:Gold; D053768:Metal Nanoparticles; D009690:Nucleic Acid Conformation; D020641:Polymorphism, Single Nucleotide; D012965:Sodium Chloride
D012965:Sodium Chloride; D006046:Gold; D004247:DNA