Establishment and maintenance of three Chinese human embryonic stem cell lines.
To establish a potential resource for cell therapy and a developmental model for human diseases, we had isolated three Chinese human embryonic stem cell lines from the inner cell mass of human blastocysts in 2002. All the three cell lines were grown on mouse embryonic fibroblasts as feeder cells; one of these cell lines, chHES-3, has maintained its normal karyotype even after being cultured in vitro for more than 100 passages, after the standardization of mouse feeder preparation. Each hES cell line has been completely characterized. All the three cell lines expressed hES-specific markers and pluripotency-related genes. These cells maintained their normal karyotype during long-term culture and displayed a high telomerase activity. When differentiated in vivo and in vitro, the derivatives representing the three germ layers could be observed. Human leukocyte antigen, ABO blood type, and DNA fingerprinting were also performed to provide a unique identity to each cell line. By establishing these hES cell lines, we provide an appropriate in vitro model to study human development and regeneration. All the three cell lines can be obtained for research purposes by placing a request at our website at www.hescbank.cn.
Zhou
Di
D
Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, Changsha, Hunan 410078, China.
Lin
Ge
G
Xie
Chang-Qing
CQ
Xiong
Bo
B
Zhou
Xiao-Ying
XY
Qian
Xiao-Bing
XB
Liao
Yi
Y
Lu
Guang-Xiu
GX
D000017:ABO Blood-Group System; D000818:Animals; D044466:Asian People; D001755:Blastocyst / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D002469:Cell Separation; D016172:DNA Fingerprinting; D053595:Embryonic Stem Cells / Q000166:cytology*; D005838:Genotype; D006650:Histocompatibility Testing; D006801:Humans; D051379:Mice
D000017:ABO Blood-Group System