Effects of fermentation conditions and soybean peptide supplementation on hyaluronic acid production by Streptococcus thermophilus strain YIT 2084 in milk.
To increase the hyaluronic acid (HA) yield from Streptococcus thermophilus YIT 2084, fermentation conditions (pH, temperature, agitation, aeration) were optimized in milk-based medium, and the effects of supplemental soybean peptides, which have different molecular weight distributions, were determined. HA production was enhanced to approximately 100 mg/l at pH 6.8 and 33-40 degrees C. Agitation speed and aeration rate slightly affected HA production. Soybean peptides including those of high molecular weight (approximately 27 to 130 kDa) further increased HA production to 208 mg/l under the optimal condition (pH 6.8, 35 degrees C, 100 rpm), which was 20-fold greater than non-optimal condition. HA production was no longer related to the specific growth rate. The HA produced under the optimal condition included a large amount of high-molecular-weight fraction of 100 to 2000 kDa, compared with under the basal condition without optimization.
Izawa
Naoki
N
Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi-shi, Tokyo, 186-8650, Japan. naoki-izawa@yakult.co.jp
Hanamizu
Tomoko
T
Sone
Toshiro
T
Chiba
Katsuyoshi
K
D000818:Animals; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods; D007700:Kinetics; D008892:Milk / Q000737:chemistry; D008970:Molecular Weight; D030262:Soybean Proteins / Q000737:chemistry; D048249:Streptococcus thermophilus / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
D003470:Culture Media; D030262:Soybean Proteins; D006820:Hyaluronic Acid