[Preparation of polyclonal antibody against human Fkbp19].
OBJECTIVE
To prepare the polyclonal antibody against human Fkbp19 for future functional studies of Fkbp19.


METHODS
Prokaryotic expression vector pET21a-Fkbp19 was transformed into E.coli BL21-DE3, and then induced by IPTG. The recombinant protein was purified by Ni-NTA resin and used to immunize rabbits. Antiserums were used for Western blot and immunofluorescence to detect Fkbp19 in breast tumor cell.


RESULTS
His-Fkbp19 fusion protein was successfully expressed in E.coli. High titer polyclonal antiserum was obtained by immunizing rabbits with purified His-Fkbp19 fusion protein. The antiserum could detect endogenous Fkbp19 protein in breast tumor cell very well by Western blot.


CONCLUSIONS
The polyclonal antiserum against Fkbp19 was successfully generated.
School of Life Science, Lanzhou University, Lanzhou, China.
Kai-ji
KJ
Fan
Yan
Y
Zhang
Nai-fa
NF
Liu
Qiang
Q
Sun
Xiao
X
Yang
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008297:Male; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D022021:Tacrolimus Binding Proteins / Q000235:genetics / Q000276:immunology*
D000906:Antibodies; C511900:FKBP11 protein, human; D011994:Recombinant Proteins; D022021:Tacrolimus Binding Proteins