Analysis of eotaxin 1/CCL11, eotaxin 2/CCL24 and eotaxin 3/CCL26 expression in lesional and non-lesional skin of patients with atopic dermatitis.
Eotaxins are the chemokines which are highly selective chemotactic agents for eosinophils. The aim of our study was the evaluation of the gene expression level for eotaxin 1/CCL11, eotaxin 2/CCL24, and eotaxin 3/CCL26, both in skin changes and in uninvolved skin of atopic dermatitis (AD) patients. The study comprised 19 patients with AD and 10 healthy controls. The gene expression level for eotaxins in the skin biopsies was evaluated by the real-time quantitative PCR. The change of the gene expression level, calculated as log10 skin lesions/non-lesional skin, was 0.635 for CCL11, 0.172 for CCL24 and 0.291 for CCL26. The change of the gene expression level, calculated as log10 non-lesional skin of AD patients/healthy control, was 0.394 for CCL11, -0.216 for CCL24, and 0.229 for CCL26, while skin lesions of AD patients/healthy control, was: 0.788, -0.046, and 0.483, respectively.


CONCLUSIONS
The mean gene expression level for CCL11, CCL24, CCL26 was higher in skin changes of AD patients than in uninvolved skin. The higher level of CCL26 in skin changes, indicates its role in their aetiology in AD. The gene expression level for CCL24 in AD patients was lower, both in involved and uninvolved skin vs. the healthy control.
Department of Dermatology, Military Institute of the Health Services, Warsaw, Poland. witold.owczarek@dermedicus.pl
Witold
W
Owczarek
Magdalena
M
Paplińska
Tomasz
T
Targowski
Karina
K
Jahnz-Rózyk
Elwira
E
Paluchowska
Aleksandra
A
Kucharczyk
Beata
B
Kasztalewicz
D000328:Adult; D016022:Case-Control Studies; D054413:Chemokine CCL11 / Q000235:genetics* / Q000378:metabolism; D054423:Chemokine CCL24 / Q000235:genetics* / Q000378:metabolism; D000075906:Chemokine CCL26; D019742:Chemokines, CC / Q000235:genetics* / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000235:genetics* / Q000473:pathology*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D055815:Young Adult
C515456:CCL11 protein, human; C515484:CCL24 protein, human; C120429:CCL26 protein, human; D054413:Chemokine CCL11; D054423:Chemokine CCL24; D000075906:Chemokine CCL26; D019742:Chemokines, CC