Quality standards in Biobanking: authentication by genetic profiling of blood spots from donor's original sample.
The field of Biobanking requires extensive work to maintain traceability of samples. However, sometimes the necessity to authenticate a sample may arise. To address these circumstances, we herein present a method for authenticating derivatives by using a blood spot from each donor, attached to a sample authentication form, by means of genetic profiling. Blood spots are collected at the time a blood sample is donated at a health centre and before processing the blood sample at the biobank. To test the validity of our approach over time, we analyzed 26 blood spots stored at room temperature in our facilities for more than 15 years. DNA was successfully extracted from the three storage materials tested in this study and 15 STR markers plus amelogenin were subsequently analyzed. The storage of a small blood spot attached to a sample authentication form proved to be efficient for genetic profiling and, therefore, may constitute a long-lasting (at least 15 years), cost-effective and effortless approach for genetic authentication of samples in biobanks.
BIOMICs Research Group, Centro de Investigación y Estudios Avanzados 'Lucio Lascaray', University of the Basque Country UPV/EHU, Vitoria-Gasteiz, Spain.
Sergio
S
Cardoso
Laura
L
Valverde
Adrian
A
Odriozola
Xabier
X
Elcoroaristizabal
Marian M
MM
de Pancorbo
D001771:Blood Banks / Q000592:standards*; D001800:Blood Specimen Collection / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D005819:Genetic Markers; D006801:Humans; D016133:Polymerase Chain Reaction; D011786:Quality Control; D011933:Reagent Kits, Diagnostic; D014019:Tissue Donors
D005819:Genetic Markers; D011933:Reagent Kits, Diagnostic