Use of in vivo imaging system-bioluminescence imaging to inspect tumor dynamic morphology.
OBJECTIVE
The aim of this study was to evaluate the veracity and sensitivity of in-vivo imaging system (IVIS) for inspection of tumor dynamic morphology.


METHODS
Mouse melanoma cells (B16-F10-luc-G5) in 100 mL media were seeded into a 96-well plate by 1:2 serial dilution from 10000 cells (well #1) to 78 cells (well #8). The plate was imaged using IVIS system to evaluate its sensitivity for luminescence. Ten Bablc mice with tumor cells were injected subcutaneously (1 x 10(5) in 100 mL) and tumor luminescence was detected by IVIS at Day 0, Day 3, Day 5, Day 7 and Day 9.


RESULTS
As few as 78 tumor cells were detectable by IVIS. A strong correlation between number of tumor cells and bioluminescence (R2=0.99) was also demonstrated. Tumor luminescence were observed in all mice by IVIS at all days, and there was significant difference (P<0.01) between each two days from Day 0 to Day 9. Moreover, tumor dynamic morphology could be monitored by IVIS when it is invisible.


CONCLUSIONS
Compared with conventional methods, with high veracity and sensitivity, IVIS system should be recommended as an effective method for inspection of tumor dynamic morphology.
Department of Radiology, the Fourth Affiliated Hospital, Harbin Medical University, No. 37 Yi Yuan Street, Harbin City, China.
Z R
ZR
Luo
T
T
Huang
W
W
Li
B Z
BZ
Shen
D000818:Animals; D003952:Diagnostic Imaging; D049449:Luminescence; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D056004:Molecular Dynamics Simulation