Prevalence and antigenic differences observed between Bovine viral diarrhea virus subgenotypes isolated from cattle in Australia and feedlots in the southwestern United States.
Bovine viral diarrhea virus (BVDV) is divided into 2 different species within the Pestivirus genus, BVDV type 1 (BVDV-1) and BVDV type 2 (BVDV-2). Further phylogenetic analysis has revealed subgenotype groupings within the 2 types. Thus far, 12 BVDV-1 subgenotypes (a-l) and 2 BVDV-2 subgenotypes (a and b) have been identified. The purpose of the current study was to determine the prevalence of BVDV subgenotypes in the United States and Australia and to determine if there are detectable antigenic differences between the prevalent subgenotypes. To determine prevalence, phylogenetic analysis was performed on 2 blinded panels of isolates consisting of 351 viral isolates provided by the Elizabeth Macarthur Laboratory, New South Wales, and 514 viral isolates provided by Oklahoma State University. Differences were observed in the prevalence of BVDV subgenotypes between the United States (BVDV-1b most prevalent subgenotype) and Australia (BVDV-1c most prevalent subgenotype). To examine antigenic differences between the subgenotypes identified in samples from the United States and Australia, polyclonal antisera was produced in goats by exposing them at 3-week intervals to 2 noncytopathic and 1 cytopathic strain of either BVDV-1a, BVDV-1b, BVDV-1c, BVDV-2a, or Border disease virus (BDV). Virus neutralization (VN) assays were then performed against 3 viruses from each of the 5 subgenotypes. Comparison of VN results suggests that there are antigenic differences between BVDV strains belonging to different subgenotypes. The present study establishes a foundation for further studies examining whether vaccine protection can be improved by basing vaccines on the BVDV subgenotypes prevalent in the region in which the vaccine is to be used.
Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 1920 Dayton Avenue, P.O. Box 70, Ames, IA 50010, USA. julia.ridpath@ars.usda.gov
Julia F
JF
Ridpath
Robert W
RW
Fulton
Peter D
PD
Kirkland
John D
JD
Neill
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000235:genetics*; D001315:Australia / Q000453:epidemiology; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology / Q000821:virology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D005838:Genotype; D009500:Neutralization Tests; D015995:Prevalence; D015206:Southwestern United States / Q000453:epidemiology
D000914:Antibodies, Viral; D000956:Antigens, Viral