[Analysis of multiple cephalosporins in blood and urine by HPLC].
OBJECTIVE
To establish a new high performance liquid chromatography (HPLC) method for determining the concentration of cefazolin, cefradine, cefoperazone and cefotaxime in blood and urine, as well as to investigate its applicability.


METHODS
Protein in blood and urine was precipitated directly by acetonitrile with acetanilide was used as the internal standard using Agilent Zorbax SB-Aq column (250 mm x 4.6 mm, 5 microm). The mixed solvents of water (triethylamine 0.12%, acetic acid 0.12%) and acetonitrile were used as the mobile phase to separate cephalosporins using gradient elution method at 1 mL/min (flow rate) and 254 nm (detection wavelength).


RESULTS
The working curve of four cephalosporins showed a good correlation (r = 0.9993), with the detection limit up to 0.01 microg/mL. The recovery rate was more than 81.2%.


CONCLUSIONS
This method is fast, easy and accurate. It is suitable for biological analysis of the 4 cephalosporins of the blood and urine in practical cases.
West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. moonlee125@163.com
Mu
M
Li
Lin-Chuan
LC
Liao
You-Yi
YY
Yan
Lin
L
Yang
Xiao-Min
XM
Liu
Jin
J
Dai
Xiao-Na
XN
Ma
Xin
X
Sheng
Hong
H
Huang
Gui
G
Su
D000328:Adult; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000652:urine*; D002437:Cefazolin / Q000097:blood / Q000652:urine; D002438:Cefoperazone / Q000097:blood / Q000652:urine; D002439:Cefotaxime / Q000097:blood / Q000652:urine; D002511:Cephalosporins / Q000097:blood* / Q000652:urine*; D002515:Cephradine / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D053593:Forensic Toxicology; D006801:Humans; D008297:Male; D012680:Sensitivity and Specificity; D013048:Specimen Handling
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D002438:Cefoperazone; D002515:Cephradine; D002437:Cefazolin; D002439:Cefotaxime