Glucose transport in interlobular ducts isolated from rat pancreas.
Pancreatic duct cells express Na(+)-dependent glucose transporter, SGLT1 and Na(+)-independent glucose transporters, GLUT1, GLUT2, and GLUT8. We examined transepithelial glucose transport by pancreatic duct. Interlobular ducts were isolated from rat pancreas. During overnight culture both ends of the duct segments sealed spontaneously. The lumen of the sealed duct was micropunctured and the luminal fluid was replaced by HEPES-buffered solution containing 10.0 mM or 44.4 mM glucose. The bath was perfused with HEPES-buffered solution at 37 degrees C containing 10.0 or 44.4 mM glucose. Transepithelial differences in osmolality were balanced with mannitol. Glucose transport across ductal epithelium was measured by monitoring changes in luminal volume. When the lumen was filled with 44.4 mM glucose, with either 10.0 or 44.4 mM glucose in the bath, the luminal volume decreased to 65 approximately 70% of the initial volume in 15 min. Luminally-injected phlorizin, an inhibitor of SGLT1, abolished the decrease in luminal volume. With 10.0 mM glucose in the lumen and 44.4 mM glucose in the bath, the luminal volume did not change significantly. Luminal application of phlorizin under identical condition led to an increase in luminal volume. The data suggest that both active and passive transport mechanisms of glucose are present in pancreatic ductal epithelium.
Futakuchi
Sachiko
S
Department of Gastroenterology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Ishiguro
Hiroshi
H
Naruse
Satoru
S
Ko
Shigeru B H
SB
Fujiki
Kotoyo
K
Yamamoto
Akiko
A
Nakakuki
Miyuki
M
Song
Ying
Y
Steward
Martin C
MC
Kondo
Takaharu
T
Goto
Hidemi
H
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism* / Q000493:pharmacokinetics; D051246:Glucose Transport Proteins, Facilitative / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000378:metabolism; D051273:Glucose Transporter Type 2 / Q000378:metabolism; D023421:Models, Animal; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism*; D051381:Rats; D051269:Sodium-Glucose Transporter 1 / Q000378:metabolism; D013311:Streptozocin
D051246:Glucose Transport Proteins, Facilitative; D051272:Glucose Transporter Type 1; D051273:Glucose Transporter Type 2; C495679:Slc2a1 protein, rat; C495683:Slc2a2 protein, rat; C498629:Slc2a8 protein, rat; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D013311:Streptozocin; D005947:Glucose