Isolation, identification and characterization of a new lipolytic pseudomonas sp., strain AHD-1, from Tunisian soil.
A novel, lipid-degrading bacterium (strain AHD-1) was isolated from soil regularly contaminated with washing-machine wastewater in Sfax, Tunisia. When this strain was grown in a medium containing 2% triacylglycerol, the hydrolysis products were found to be diacylglycerols, monoacylglycerols and free fatty acids. This strain was an aerobic, mesophilic, Gram-negative, motile, non-sporulating bacterium, capable of growing optimally at pH 7 and 27 degrees C. The predominant fatty acids were found to be C16:1omega7c (31%), C16:0 (28.1%), C18:1 omega7c (16.3%) and C17:0 (5.8%). Phylogenetic analysis of the 16S rRNA gene showed that this isolate is a new strain belonging to the genus Pseudomonas. Strain AHD-1 was found to be closely related to Pseudomonas azotoformans IAM 1603T, Pseudomonas gessardii CIP 105469T and Pseudomonas libanensis CIP 105460T with 99.7%, 99.56% and 99.54% of similarity, respectively.
Laboratoire des Bioprocédés, Pôle d'Excellence Régionale A UF, (PER-LBP), Centre de Biotechnologie de Sfax, Sfax, Tunisia.
Imen
I
Fendri
Ali
A
Chaari
Abdelhafidh
A
Dhouib
Brahim
B
Jlassi
Abdelkarim
A
Abousalham
Frédéric
F
Carrière
Sami
S
Sayadi
Slim
S
Abdelkafi
D050356:Lipid Metabolism / Q000502:physiology*; D008066:Lipolysis / Q000502:physiology*; D011549:Pseudomonas / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology*; D012988:Soil Microbiology; D013045:Species Specificity; D014416:Tunisia