Quantitative proteomics analysis of Streptomyces coelicolor development demonstrates that onset of secondary metabolism coincides with hypha differentiation.
Streptomyces species produce many clinically important secondary metabolites, including antibiotics and antitumorals. They have a complex developmental cycle, including programmed cell death phenomena, that makes this bacterium a multicellular prokaryotic model. There are two differentiated mycelial stages: an early compartmentalized vegetative mycelium (first mycelium) and a multinucleated reproductive mycelium (second mycelium) arising after programmed cell death processes. In the present study, we made a detailed proteomics analysis of the distinct developmental stages of solid confluent Streptomyces coelicolor cultures using iTRAQ (isobaric tags for relative and absolute quantitation) labeling and LC-MS/MS. A new experimental approach was developed to obtain homogeneous samples at each developmental stage (temporal protein analysis) and also to obtain membrane and cytosolic protein fractions (spatial protein analysis). A total of 345 proteins were quantified in two biological replicates. Comparative bioinformatics analyses revealed the switch from primary to secondary metabolism between the initial compartmentalized mycelium and the multinucleated hyphae.
double daggerProtein Research Group, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. mantecaangel@uniovi.es
Angel
A
Manteca
Jesus
J
Sanchez
Hye R
HR
Jung
Veit
V
Schwämmle
Ole N
ON
Jensen
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016000:Cluster Analysis; D025301:Hyphae / Q000254:growth & development*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D048372:Streptomyces coelicolor / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D001426:Bacterial Proteins; D020543:Proteome