Human anti-60 kD heat shock protein autoantibodies are characterized by basic features of natural autoantibodies.
Anti-human Hsp60 autoantibodies--known risk factor of atherosclerosis--were investigated in a mouse model and in samples of healthy subjects: polyreactivity, presence in cord blood samples of healthy newborns and life-long stability were tested. In IgM hybridoma panel from mouse spleens, polyreactivity of anti-Hsp60 autoantibodies was studied. In healthy pregnant women, umbilical vein and maternal blood samples were collected after childbirth, anti-Hsp-60 and -65 IgM and IgG levels were measured. Life-long stability of anti-Hsp-60 levels was studied on healthy patients during 5 years. ELISA was used in all studies. Polyreactivity of IgM clones of newborn mice and lifelong stability of these autoantibodies in healthy adults were established. IgM anti-Hsp60 autoantibodies in cord blood of healthy human infants were present, however, there was no correlation between maternal and cord blood IgM anti-Hsp60 concentrations. It is proposed that presence of anti-Hsp60 autoantibodies--as part of the natural autoantibody repertoire--may be an inherited trait. Level of anti-Hsp60 autoantibodies may be an independent, innate risk factor of atherosclerosis for the adulthood.
Varbiro
Szabolcs
S
2nd Department of Obstetrics and Gynecology, Faculty of Medicine, Semmelweis University, Budapest, Hungary. varbiroszabolcs@gmail.com
Biro
A
A
Cervenak
J
J
Cervenak
L
L
Singh
M
M
Banhidy
F
F
Sebestyen
A
A
Füst
G
G
Prohászka
Z
Z
D000328:Adult; D000818:Animals; D000831:Animals, Newborn; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000276:immunology*; D018834:Chaperonin 60 / Q000276:immunology*; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D005500:Follow-Up Studies; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D011247:Pregnancy; D055815:Young Adult
D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D018834:Chaperonin 60; C044203:anti-IgM