Prokaryotic ubiquitin-like protein provides a two-part degron to Mycobacterium proteasome substrates.
Prokaryotic ubiquitin-like protein (Pup) is a posttranslational modifier that targets proteins for degradation by the mycobacterial proteasome. We show that the disordered amino terminus of Pup is required for degradation, while the helical carboxyl terminus mediates its attachment to proteins. Thus, Pup has distinct regions that either interact with pupylation enzymes or initiate proteasomal degradation.
New York University School of Medicine, Department of Microbiology, 550 First Avenue, MSB 236, New York, NY 10016, USA.
Kristin E
KE
Burns
Michael J
MJ
Pearce
K Heran
KH
Darwin
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D009161:Mycobacterium / Q000235:genetics / Q000378:metabolism*; D020102:Mycobacterium smegmatis / Q000235:genetics / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; C533288:Pup protein, Mycobacterium tuberculosis; D014452:Ubiquitins; D046988:Proteasome Endopeptidase Complex