[X-sNP genotyping using the TaqMan probe technology].
OBJECTIVE
To develop a rapid, accurate and economical real time fluorescence PCR method with TaqMan probe technology to detect the X chromosome single nucleotide polymorphism (X-SNP).


METHODS
TaqMan probes and polymerase chain reaction primers were respectively designed according to the 13 X-SNP. Then, the X-SNP were genotyped after the amplification by real time fluorescence PCR.


RESULTS
All the loci follow the Hardy-Weinberg equilibrium. The polymorphic information content for 13 distinct loci varied between 0.3497 and 0.3750 while the heterozygosity ranged from 0.4537 to 0.5021. A real time fluorescent PCR method based on TaqMan probe was successfully developed and the results were accordant with those analyzed by DNA sequencing of the 13 X-SNP.


CONCLUSIONS
The allele specific real time fluorescence PCR based on TaqMan probe is a sensitive, simple technology and suitable for rapid analysis of XSNP. All the loci show highly polymorphic and may be potential in forensic genetics.
Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China. zsh-daisy@163.com
Su-hua
SH
Zhang
Li
L
Li
Cheng-tao
CT
Li
Shu-min
SM
Zhao
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D041321:Chromosomes, Human, X / Q000235:genetics*; D004247:DNA / Q000097:blood / Q000235:genetics; D015342:DNA Probes; D005260:Female; D005456:Fluorescent Dyes; D053803:Forensic Genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase
D015342:DNA Probes; D005456:Fluorescent Dyes; D004247:DNA; D019914:Taq Polymerase