Metabolic analysis of elicited cell suspension cultures of Cannabis sativa L. by (1)H-NMR spectroscopy.
Cannabis sativa L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with jasmonic acid (JA) and pectin as elicitors to evaluate their effect on metabolism from two cell lines using NMR spectroscopy and multivariate data analysis. According to principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA), the chloroform extract of the pectin-treated cultures were more different than control and JA-treated cultures; but in the methanol/water extract the metabolome of the JA-treated cells showed clear differences with control and pectin-treated cultures. Tyrosol, an antioxidant metabolite, was detected in cannabis cell cultures. The tyrosol content increased after eliciting with JA.
Faculty of Pharmacy, Pharmacognosy Department, Charles University in Prague, Heyrovského 1203, 500 05, Hradec Králové, Czech Republic.
Jaroslav
J
Pec
Isvett Josefina
IJ
Flores-Sanchez
Young Hae
YH
Choi
Robert
R
Verpoorte
D002188:Cannabis / Q000737:chemistry* / Q000378:metabolism*; D002457:Cell Extracts / Q000737:chemistry* / Q000302:isolation & purification; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome; D054883:Oxylipins / Q000378:metabolism; D010368:Pectins / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000032:analysis
D002457:Cell Extracts; D003517:Cyclopentanes; D054883:Oxylipins; C011867:4-hydroxyphenylethanol; C011006:jasmonic acid; D010368:Pectins; D010626:Phenylethyl Alcohol