Pyrrolomycins as potential anti-staphylococcal biofilms agents.
With the goal of discovering new anti-infective agents active against microbial biofilms, this investigation focused on some natural pyrrolomycins, a family of halogenated pyrrole antibiotics. In this study the anti-staphylococcal biofilm activity of pyrrolomycins C, D, F1, F2a, F2b, F3 and of the synthesized related compounds I, II, III were investigated. The susceptibility of six staphylococcal biofilms was determined by methyltiazotetrazolium staining. Most of the compounds were active at concentrations of 1.5 microg ml(-1) with significant inhibition percentages. A few of the compounds were active at the lowest screening concentration of 0.045 microg ml(-1). The population log reduction of activity against the two best biofilm forming Staphylococcus aureus strains as determined by viable plate counts is also reported. In order to adequately assess the utility of these compounds, their toxicity against human cells was evaluated. It is concluded that pyrrolomycins and synthetic derivatives are promising compounds for developing novel effective chemical countermeasures against staphylococcal biofilms.
Schillaci
Domenico
D
Department of Medicinal Chemistry and Technology, Universita degli Studi di Palermo, Palermo, Italy.
Petruso
Salvatore
S
Raimondi
Maria Valeria
MV
Cusimano
Maria Grazia
MG
Cascioferro
Stella
S
Scalisi
Marianna
M
La Giglia
Maria Anna
MA
Vitale
Maria
M
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects / Q000235:genetics; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D016133:Polymerase Chain Reaction; D011758:Pyrroles / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology
D000900:Anti-Bacterial Agents; D011758:Pyrroles