Phylogenetic relationships and diversity of β-rhizobia associated with Mimosa species grown in Sishuangbanna, China.
In order to investigate the genetic diversity of rhizobia associated with various exotic and invasive species in tropical mainland China, 116 bacterial isolates were obtained from Mimosa root nodules collected from Sishuangbanna and Yuanjiang districts of Yunnan province. Isolated rhizobia were characterized by RFLP analysis of 16S rRNA genes, SDS-PAGE of whole-cell proteins and BOX-PCR. Most of the isolated strains were identified as β-rhizobia belonging to diverse populations of Burkholderia and Cupriavidus, and the phylogenetic relationships of their 16S rRNA gene sequences showed that they were closely related to one of four β-rhizobia species: Burkholderia phymatum, B. mimosarum, B. caribensis or Cupriavidus taiwanensis. Additionally, among the 116 isolates, 53 different whole-cell SDS-PAGE profiles and 30 distinct BOX-PCR genotypic patterns were detected, which demonstrated the genetic and phenotypic diversity found within these Burkholderia and Cupriavidus strains. To the best of our knowledge, this is the first report that β-rhizobia are extant and possibly widespread on the Chinese mainland and nodulate easily with Mimosa plants. We also find it especially interesting that this appears to be the first report from mainland China of Cupriavidus symbionts of Mimosa. These records enrich our knowledge and understanding of the geographical distribution and diversity of these bacteria.
Liu
Xiao Yun
XY
Key Laboratory of Biodiversity Conservation in Southwest China in the State Forestry Administration, Southwest Forestry College, Kunming 650224, PR China.|Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 100072, PR China.
Wu
Wei
W
Key Laboratory of Biodiversity Conservation in Southwest China in the State Forestry Administration, Southwest Forestry College, Kunming 650224, PR China.
Wang
En Tao
ET
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México DF 13410, Mexico.|College of Biological Sciences, China Agricultural University, Beijing 100094, PR China.
Zhang
Bin
B
Key Laboratory of Biodiversity Conservation in Southwest China in the State Forestry Administration, Southwest Forestry College, Kunming 650224, PR China.
Macdermott
Jomo
J
Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 100072, PR China.
Chen
Wen Xin
WX
College of Biological Sciences, China Agricultural University, Beijing 100094, PR China.
D019117:Burkholderia / Q000145:classification / Q000235:genetics*; D002681:China; D016000:Cluster Analysis; D049921:Cupriavidus / Q000145:classification / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D005838:Genotype; D058865:Introduced Species; D031298:Mimosa / Q000382:microbiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013559:Symbiosis
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S