Beneficial effect of Berberis buxifolia Lam, Ziziphus mistol Griseb and Prosopis alba extracts on oxidative stress induced by chloramphenicol.
The chemiluminescence of luminol, a measure of oxidative stress, increased immediately as a consequence of reactive oxygen species (ROS) stimulated by this antibiotic. The effect of Ch was dose dependent with maximum stimulus at 8 mg/ml (Vmax); above this concentration the cells began to reduce the production of ROS. The oxidative injury of Ch was counteracted by water extracts of Berberis buxifolia lam, Zizyphus mistol Griseb and Prosopis alba, indigenous fruits from Argentina. The relatively light units (RLU) emitted decreased immediately as a consequence of a protective effect exerted by the extracts of these fruit extracts on blood cells. The three indigenous fruit extracts reduced to a different extent the oxidative injury caused by Ch. B.buxifolia lam exhibited the highest antioxidant capacity followed by Z.mistol Griseb. Water extracts of both fruit extracts were the most effective against the oxidative stress, while P.alba presented better antioxidant capacity in the ethanolic fraction obtained. Hexane extracts showed low protective action on blood cells, with little reduction of area under curve (AUC) of RLU plotted versus time. Leukocytes remained viable in blood samples incubated for 3h with Ch and water extracts of B. buxifolia lam or Z. mistol Griseb (97.1% and 92.5% viability by Trypan blue exclusion, respectively); whereas with Ch only the cells were stressed and viability decreased to 30%. The three fruit extracts protected the viability of leukocytes in parallel with the decrease of ROS. Erythrocytes were not lysed in the presence of Ch.
Albrecht
Claudia
C
Escuela de Nutrición, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba.
Pellarin
Gabriela
G
Rojas
María José
MJ
Albesa
Inés
I
Eraso
Alberto F
AF
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D028142:Berberis / Q000737:chemistry; D002701:Chloramphenicol / Q000494:pharmacology*; D019610:Cytoprotection / Q000187:drug effects; D006801:Humans; D007962:Leukocytes / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D029862:Prosopis / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000097:blood; D031957:Ziziphus / Q000737:chemistry
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D002701:Chloramphenicol