Antibacterial activity of dental composites containing zinc oxide nanoparticles.
The resin-based dental composites commonly used in restorations result in more plaque accumulation than other materials. Bacterial biofilm growth contributes to secondary caries and failure of resin-based dental composites. Methods to inhibit biofilm growth on dental composites have been sought for several decades. It is demonstrated here that zinc oxide nanoparticles (ZnO-NPs) blended at 10% (w/w) fraction into dental composites display antimicrobial activity and reduce growth of bacterial biofilms by roughly 80% for a single-species model dental biofilm. Antibacterial effectiveness of ZnO-NPs was assessed against Streptococcus sobrinus ATCC 27352 grown both planktonically and as biofilms on composites. Direct contact inhibition was observed by scanning electron microscopy and confocal laser scanning microscopy while biofilm formation was quantified by viable counts. An 80% reduction in bacterial counts was observed with 10% ZnO-NP-containing composites compared with their unmodified counterpart, indicating a statistically significant suppression of biofilm growth. Although, 20% of the bacterial population survived and could form a biofilm layer again, 10% ZnO-NP-containing composites maintained at least some inhibitory activity even after the third generation of biofilm growth. Microscopy demonstrated continuous biofilm formation for unmodified composites after 1-day growth, but only sparsely distributed biofilms formed on 10% ZnO-NP-containing composites. The minimum inhibitory concentration of ZnO-NPs suspended in S. sobrinus planktonic culture was 50 microg mL(-1). ZnO-NP-containing composites (10%) qualitatively showed less biofilm after 1-day-anaerobic growth of a three-species initial colonizer biofilm after being compared with unmodified composites, but did not significantly reduce growth after 3 days.
Aydin Sevinç
Berdan
B
Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607-7061, USA.
Hanley
Luke
L
D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D015169:Colony Count, Microbial; D003188:Composite Resins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D053758:Nanoparticles / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry / Q000494:pharmacology*; D015034:Zinc Oxide / Q000737:chemistry*
D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; C029012:Composite Dental Resin; D003188:Composite Resins; D011140:Polyurethanes; D015034:Zinc Oxide