Measurement of urinary F2-isoprostanes as markers of in vivo lipid peroxidation: a comparison of enzyme immunoassays with gas chromatography-mass spectrometry in domestic animal species.
F(2)-isoprostanes are useful markers for assessing oxidant injury; however, the validity of measuring urinary 15-F(2t)-isoprostane concentration by enzyme-linked immunosorbent assay (ELISA) has not been evaluated in veterinary species. The current study assesses the agreement between 2 commercially available urinary isoprostane kits and gas chromatography and negative ion chemical ionization-mass spectrometry (GC/NICI-MS). The results indicate that only feline urinary isoprostane measurement by glucuronidase (GL)-ELISA has acceptable agreement with GC/NICI-MS. Urinary isoprostane concentration was highly variable in critically ill animals, but there were too many variations between healthy and critically ill animals to draw meaningful conclusions. Currently, GC/NICI-MS is the only method that can be recommended for the assessment of urinary isoprostanes in dogs, cattle, and horses. Feline urinary isoprostanes can be assessed by GL-ELISA, but caution is still warranted when comparing data from manuscripts using different methods given the relatively low Spearman rank correlation coefficient. Future studies may require large sample sizes or focused inclusion criteria to account for variability in isoprostane concentration.
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Carl
C
Soffler
Vicki L
VL
Campbell
Diana M
DM
Hassel
D000820:Animal Diseases / Q000652:urine; D000818:Animals; D002415:Cats; D002417:Cattle; D016638:Critical Illness; D004285:Dogs; D028441:F2-Isoprostanes / Q000652:urine*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000662:veterinary*; D006736:Horses / Q000652:urine; D007124:Immunoenzyme Techniques / Q000662:veterinary*; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male
D028441:F2-Isoprostanes