[Puerarin antagonizes activation effect of LPS on N9 microglia cells].
OBJECTIVE
To investigate the effect of puerarin on lipopolysaccharide (LPS)-activated N9 microglia cells.


METHODS
MTT was used to detect the cytotoxic effect of puerarin and LPS on N9 microglia cells. Flow cytometry was used to determine the effect of puerarin on cellular production of nitric oxide (NO) and reactive oxygen species (ROS), apoptosis and cell cycle in LPS-activated N9 microglia cells. The amount of NO released into the supernatant was detected by Griess assay. The HE staining was used to show the morphological response of LPS-activated N9 microglia cells to puerarin.


RESULTS
Morphologically, amoeba-like LPS-activated N9 cells turned to be round in quiescence after treated with puerarin (100-200 micromol/L). Puerarin (50-200 micromol/L) caused less amount of NO released into the supernatant, with 12.43+/-0.11 micromol/L of NO at a dose of 200 micromol/L puerarin compared with 23.45+/-0.19 micromol/L in untreated control (P<0.01). Likewise, puerarin (200 micromol/L) completely abolished the ROS-producing ability of LPS-activated N9 cells, with a basal ROS level comparable to the non-LPS-treated control. Puerarin also exerted an anti-apoptotic effect on the LPS-activated N9 cells and rescued the cells from G(0)/G(1) arrest.


CONCLUSIONS
Puerarin antagonizes activation effect of LPS on N9 microglia cells and might be a useful drug for the treatment of neurodegenerative disorders.
Bai
Qun-hua
QH
Institute of Life Sciences, Chongqing Medical University, Chongqing, China. baiqunh_744111@sina.com
Li
Wen-ming
WM
Liu
Hong-tao
HT
Chen
Wen-juan
WJ
Wang
Yang
Y
Yu
Chao
C
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D007529:Isoflavones / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
D007529:Isoflavones; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; C033607:puerarin