Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos.
Chimera formation is a powerful tool for analyzing pluripotency in vivo. It has been widely accepted that host cell lineages are generally accessible to embryonic stem (ES) cells with the actual contribution depending solely on the intrinsic pluripotency of transplanted donor cells. Here, we show in the fish medaka (Oryzias latipes) that the host accessibility to ES cell contribution exhibits dramatic differences. Specifically, of three albino host strains tested (i (1) , i (3) and af), only strain i (1) generated pigmented chimeras. Strikingly, this accessibility is completely lost in i (1) but acquired in i (3) after host gamma-irradiation. Host irradiation also differentially affected ES cell contribution to somatic organs and gonad. Therefore, the accessibility of various host cell lineages can vary considerably depending on host strains and cell lineages as well as on irradiation. Our findings underscore the importance of host genotypes for interpreting donor cell pluripotency and for improving ES-derived chimera production.
Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260, Singapore.
Ni
N
Hong
Mingyou
M
Li
Zhiqiang
Z
Zeng
Meisheng
M
Yi
Jiaorong
J
Deng
Jianfang
J
Gui
Christoph
C
Winkler
Manfred
M
Schartl
Yunhan
Y
Hong
D000818:Animals; D019070:Cell Lineage / Q000235:genetics; D002478:Cells, Cultured; D002678:Chimera; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000637:transplantation*; D005720:Gamma Rays; D005838:Genotype; D008164:Luminescent Proteins / Q000378:metabolism; D009990:Oryzias / Q000196:embryology*; D010858:Pigmentation; D000097573:Red Fluorescent Protein
D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein