Costal strip production and lorica assembly in the large tectiform choanoflagellate Diaphanoeca grandis Ellis.
Diaphanoeca grandis posseses a voluminous flask-shaped lorica comprising an outer layer of 12 longitudinal costae and an inner layer of four transverse costae. The cell is suspended just above the centre of the lorica chamber by tentacles that are attached to the anterior transverse ring. The component costal strips are superficially similar although four different strip categories can be distinguished on the basis of length and morphology. Costal strips are produced 'upside-down' within the parent cell and accumulated in a close-packed horizontal ring at the top of the inner surface of the collar. The order in which costal strips are produced is consistent, starting with those for the transverse rings, basal to anterior, and then the longitudinal costae, again with the posterior first and the anterior later. Cell division is of the classical tectiform variety with the juvenile cell being inverted and pushed backwards out of the parent lorica. Lorica assembly entails firstly the rotation of the anterior vertical strips so they become horizontal and then their movement backwards under the posterior layer of longitudinal strips. From this time onwards, lorica assembly proceeds in a standard manner with the lorica-assembling tentacles providing a forward and left-handed rotational movement.
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. B.S.C.Leadbeater@bham.ac.uk
Barry S C
BS
Leadbeater
Rong
R
Cheng
D000818:Animals; D002455:Cell Division; D056897:Choanoflagellata / Q000502:physiology* / Q000648:ultrastructure; D003599:Cytoskeleton / Q000378:metabolism*; D008853:Microscopy; D046529:Microscopy, Electron, Transmission; D015388:Organelles / Q000648:ultrastructure*