A functional extracellular matrix biomaterial derived from ovine forestomach.
Extracellular matrix (ECM) based biomaterials have an established place as medical devices for wound healing and tissue regeneration. In the search for biomaterials we have identified ovine forestomach matrix (OFM), a thick, large format ECM which is biochemically diverse and biologically functional. OFM was purified using an osmotic process that was shown to reduce the cellularity of the ECM and aid tissue delamination. OFM produced using this technique was shown to retain residual basement membrane components, as evidence by the presence of laminin and collagen IV. The collagenous microarchitecture of OFM retained many components of native ECM including fibronectin, glycosaminoglycans, elastin and fibroblast growth factor basic. OFM was non-toxic to mammalian cells and supported fibroblast and keratinocyte migration, differentiation and infiltration. OFM is a culturally acceptable alternative to current collagen-based biomaterials and has immediate clinical applications in wound healing and tissue regeneration.
Lun
Stan
S
Mesynthes Limited, Lower Hutt 5040, New Zealand.
Irvine
Sharleen M
SM
Johnson
Keryn D
KD
Fisher
Neil J
NJ
Floden
Evan W
EW
Negron
Leonardo
L
Dempsey
Sandi G
SG
McLaughlin
Rene J
RJ
Vasudevamurthy
Madhusudan
M
Ward
Brian R
BR
May
Barnaby C H
BC
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000378:metabolism; D016716:PC12 Cells; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D012038:Regeneration / Q000502:physiology; D012756:Sheep; D013270:Stomach / Q000033:anatomy & histology / Q000737:chemistry*
D001672:Biocompatible Materials; D007797:Laminin; D020033:Protein Isoforms; D016222:Fibroblast Growth Factor 2; D003094:Collagen