Crevicular fluid matrix metalloproteinase-8, -13, and TIMP-1 levels in type 2 diabetics.
OBJECTIVE
To evaluate whether type 2 diabetes mellitus (DM) enlarged and if so the quantum of such increase in the gingival crevicular fluid (GCF) levels of matrix metalloproteinase-8 (MMP-8), MMP-13 and tissue inhibitor of metalloproteinases-1 (TIMP-1).


METHODS
Subjects (n = 73) were divided into five groups as follows: 12 DM patients with gingivitis (DM-G), 12 DM patients with periodontitis (DM-P), 12 systemically healthy patients with gingivitis (H-G), 13 systemically healthy patients with periodontitis (H-P) and 24 periodontally, systemically healthy volunteer subjects (H-C). Full-mouth clinical periodontal measurements were performed at six sites per tooth. Gingival crevicular fluid samples were obtained from two sites representing the clinical periodontal diagnosis in single-rooted teeth. Gingival crevicular fluid levels of MMP-8, MMP-13 and TIMP-1 were analysed by immunofluorometric MMP assay (IFMA), enzyme-linked immunosorbent assay (ELISA). Data were tested statistically by parametric tests.


RESULTS
All clinical periodontal measurements were similar in both diabetic and systemically healthy patients with periodontal disease (all P > 0.05). Total amounts of MMP-8 in GCF samples were significantly lower in H-C group than DM-G, DM-P, H-P groups (all P < 0.05). Matrix metalloproteinase-13, TIMP-1 total amounts were similar in study groups (P > 0.05). Diabetes mellitus patients exhibited similar levels of MMP-8, MMP-13, TIMP-1 with systemically healthy gingivitis/periodontitis patients (P > 0.05).


CONCLUSIONS
Within the limits of this study, DM does not seem to significantly affect GCF levels of MMP-8, MMP-13, TIMP-1 or clinical periodontal status.
Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey.
L
L
Kardeşler
B
B
Biyikoğlu
S
S
Cetinkalp
M
M
Pitkala
T
T
Sorsa
N
N
Buduneli
D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D016022:Case-Control Studies; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D055113:Chronic Periodontitis / Q000150:complications / Q000201:enzymology; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000201:enzymology*; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D005884:Gingival Hemorrhage / Q000150:complications / Q000201:enzymology; D005891:Gingivitis / Q000150:complications / Q000201:enzymology; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000032:analysis*; D020784:Matrix Metalloproteinase 8 / Q000032:analysis*; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000150:complications / Q000201:enzymology; D010514:Periodontal Pocket / Q000150:complications / Q000201:enzymology; D012907:Smoking; D013997:Time Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis*; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D002784:Cholesterol; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D006442:Glycated Hemoglobin; D053509:Matrix Metalloproteinase 13; D020784:Matrix Metalloproteinase 8; D019715:Tissue Inhibitor of Metalloproteinase-1; D014280:Triglycerides; C507079:MMP13 protein, human; C488762:MMP8 protein, human