Replenishment of type VII collagen and re-epithelialization of chronically ulcerated skin after intradermal administration of allogeneic mesenchymal stromal cells in two patients with recessive dystrophic epidermolysis bullosa.
In animal models it has been shown that mesenchymal stromal cells (MSC) contribute to skin regeneration and accelerate wound healing. We evaluated whether allogeneic MSC administration resulted in an improvement in the skin of two patients with recessive dystrophic epidermolysis bullosa (RDEB; OMIM 226600). Patients had absent type VII collagen immunohistofluorescence and since birth had suffered severe blistering and wounds that heal with scarring. Vehicle or 0.5 x 10(6) MSC were infused intradermally in intact and chronic ulcerated sites. One week after intervention, in MSC-treated skin type VII collagen was detected along the basement membrane zone and the dermal-epidermal junction was continuous. Re-epithelialization of chronic ulcerated skin was observed only near MSC administration sites. In both patients the observed clinical benefit lasted for 4 months. Thus intradermal administration of allogeneic MSC associates with type VII collagen replenishment at the dermal-epidermal junction, prevents blistering and improves wound healing in unconditioned patients with RDEB.
Facultad de Medicina, Clinica Alemana Universidad del Desarrollo, Santiago, Chile. pconget@udd.cl
Paulette
P
Conget
Fernando
F
Rodriguez
Susanne
S
Kramer
Carolina
C
Allers
Valeska
V
Simon
Francis
F
Palisson
Sergio
S
Gonzalez
Maria J
MJ
Yubero
D000293:Adolescent; D000328:Adult; D000818:Animals; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D024143:Collagen Type VII / Q000235:genetics / Q000378:metabolism*; D016108:Epidermolysis Bullosa Dystrophica / Q000235:genetics / Q000473:pathology / Q000628:therapy; D005260:Female; D006801:Humans; D007271:Injections, Intradermal; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D012867:Skin / Q000473:pathology*; D014184:Transplantation, Homologous
D024143:Collagen Type VII