E-selectin targeting to visualize tumors in vivo.
Generally angiogenic factors induce the expression of E-selectin in vascular endothelial cells in the tumors. In this study, we employed an anti-E-selectin monoclonal antibody to target tumors in vivo and evaluated an optical imaging reagent to visualize tumor regions. The anti-E-selectin antibody was conjugated on the surface of liposomes, which encapsulated the near-infrared fluorescent substances Cy3 or Cy5.5. The liposomes efficiently recognized human umbilical vein endothelial cells only when E-selectin was induced by angiogenic factors such as TNF-alpha in vitro. Cy5.5 encapsulated into liposomes that were conjugated with an anti-E-selectin antibody successfully visualized Ehrlich ascites tumor cells when transplanted into mice. Thus, E-selectin targeting with liposomes containing a near-infrared fluorescent dye was found effective in visualizing tumors in vivo. This strategy should be extremely useful as a method to identify sentinel lymphatic nodes and angiogenic tumors as well as use for drug delivery to tumor cells.
Hirai
Masahiko
M
Katayama Chemical Industries Co. Ltd, Minoh, Osaka, Japan.
Hiramatsu
Yoshie
Y
Iwashita
Shinki
S
Otani
Takayuki
T
Chen
Ling
L
Li
Yue-guang
YG
Okada
Masashi
M
Oie
Kazunori
K
Igarashi
Koich
K
Wakita
Hideaki
H
Seno
Masaharu
M
D000818:Animals; D000911:Antibodies, Monoclonal; D002286:Carcinoma, Ehrlich Tumor / Q000175:diagnosis; D019040:E-Selectin / Q000276:immunology*; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular; D006801:Humans; D018796:Immunoconjugates; D008081:Liposomes; D051379:Mice; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000175:diagnosis; D014471:Umbilical Veins
D000911:Antibodies, Monoclonal; D019040:E-Selectin; D018796:Immunoconjugates; D008081:Liposomes