Molecular replacement studies on crystal structure of DesB1-B2 Despentapeptide (B26-B30) insulin.
Using the crystal structure of Despentapeptide (B26-B30) insulin (DPI as the search model, the crystal structure of DesB1-B2 Despentapeptide (B26-B30) insulin (DesB1-2 DPI) has been studied by the molecular replacement method. There is one DesB1-2 DPI molecule in each crystallographic asymmetric unit. The cross rotation function search and the translation function search show apparent peaks and thus determine the orientation and position of DesB1-2 DPI molecule in the cell respectively. The subsequent three-dimensional structural rebuilding and refinement of DesB1-2 DPI molecule confirm the results by molecular replacement method.
State Key Laboratory of Biornacrornolecules, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China.
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Diao
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Wan
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Chang
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Liang