Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line.
A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression.
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, Kita-Ku, Okayama 700-8530, Japan.
Masamichi
M
Kajita
Masaki
M
Magari
Kagefumi
K
Todo
Naoki
N
Kanayama
Hitoshi
H
Ohmori
D000818:Animals; D030161:Avian Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D005785:Gene Conversion; D015870:Gene Expression; D018390:Gene Targeting; D005803:Genes, Immunoglobulin; D005818:Genetic Engineering; D007136:Immunoglobulins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D017354:Point Mutation
D030161:Avian Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D007136:Immunoglobulins; C110901:X-ray repair cross complementing protein 3