Derivation, culture, and characterization of VUB hESC lines.
In this report, we present the derivation and characterization of 15 hESC lines established at the Vrije Universiteit Brussel, Belgium in collaboration with the Universitair Ziekenhuis Brussel, Belgium, using surplus in vitro fertilization embryos and embryos carrying monogenic disorders donated for research. Four lines were derived from blastocyst-stage embryos presumed to be genetically normal, and 11 hESC lines were obtained from embryos shown to carry genetic mutations by preimplantation genetic diagnosis. All the lines express markers of pluripotency as determined by immunocytochemistry and RT-PCR, and formed teratomas when injected into SCID mice. All VUB hESC lines, except for VUB17, are reported in the European hESC registry and are available upon request after signing a Material Transfer Agreement from the VUB (contact person: Prof. Dr. Karen Sermon; Karen.Sermon@uzbrussel.be).
Mateizel
Ileana
I
Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium. ileana.mateizel@uzbrussel.be
Spits
Claudia
C
De Rycke
Martine
M
Liebaers
Inge
I
Sermon
Karen
K
D000818:Animals; D001530:Belgium; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line / Q000166:cytology*; D004194:Disease; D033041:Embryo Research; D004622:Embryo, Mammalian / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D005820:Genetic Testing; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D014495:Universities