Chromosome wide analysis of CUGBP1 binding sites identifies the tetraspanin CD9 mRNA as a target for CUGBP1-mediated down-regulation.
CUGBP1 is an RNA-binding protein controlling alternative splicing, mRNA translation and stability. In this work we used a motif scoring approach to identify putative CUGBP1 binding sites for genes located on the human chromosome 12. This allowed us to identify the gene CD9 as a presumptive target for CUGBP1-mediated regulation. In a number of cancers, the tetraspanin CD9 is down-regulated, an event correlated with a bad prognostic. Using a combination of biochemical approaches and CUGBP1 knockdown, we showed that CUGBP1 directly controls CD9 expression.
Le Tonquèze
Olivier
O
CNRS, UMR 6061 Institut de Génétique et Développement de Rennes, Expression Génétique et Développement, Université de Rennes I, IFR 140 GFAS, 2 avenue du Professeur Léon Bernard, CS 34317, 35043 Rennes Cedex, France.
Gschloessl
Bernhard
B
Namanda-Vanderbeken
Allen
A
Legagneux
Vincent
V
Paillard
Luc
L
Audic
Yann
Y
D020413:3' Untranslated Regions; D015703:Antigens, CD / Q000235:genetics*; D001665:Binding Sites; D000067879:CELF1 Protein; D002478:Cells, Cultured; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D015536:Down-Regulation; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA / Q000379:methods; D060245:Tetraspanin 29
D020413:3' Untranslated Regions; D015703:Antigens, CD; C554869:CD9 protein, human; D000067879:CELF1 Protein; C102843:CELF1 protein, human; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D060245:Tetraspanin 29