Extension of the core map of common bean with EST-SSR, RGA, AFLP, and putative functional markers.
Microsatellites and gene-derived markers are still underrepresented in the core molecular linkage map of common bean compared to other types of markers. In order to increase the density of the core map, a set of new markers were developed and mapped onto the RIL population derived from the 'BAT93' x 'Jalo EEP558' cross. The EST-SSR markers were first characterized using a set of 24 bean inbred lines. On average, the polymorphism information content was 0.40 and the mean number of alleles per locus was 2.7. In addition, AFLP and RGA markers based on the NBS-profiling method were developed and a subset of the mapped RGA was sequenced. With the integration of 282 new markers into the common bean core map, we were able to place markers with putative known function in some existing gaps including regions with QTL for resistance to anthracnose and rust. The distribution of the markers over 11 linkage groups is discussed and a newer version of the common bean core linkage map is proposed.
Luiz Ricardo
LR
Hanai
Luciane
L
Santini
Luis Eduardo Aranha
LE
Camargo
Maria Helena Pelegrinelli
MH
Fungaro
Paul
P
Gepts
Siu Mui
SM
Tsai
Maria Lucia Carneiro
ML
Vieira