[Determination of ethyl glucuronide in blood by GC-MS/MS].
OBJECTIVE
To develop a method for determining ethyl glucuronide(EtG) in human blood with gas chromatograph-tandem mass spectrometry (GC-MS/MS).


METHODS
Human blood protein was precipitated with acetonitrile. The supernatant was transferred and air flow dried after centrifugated. The residue was derived with N, O-bis (trimethylsilyl)trifluoroacetamide (BSTFA), and analyzed with GC-MS/MS.


RESULTS
The detection limit of EtG in blood was 0.05 microg/mL. Calibration curve covered a span from 0.1-10 microg/mL with a good linear relationship (r = 0.999 9). The method showed a excellent performance when was used to authentic blood sample analysis for EtG.


CONCLUSIONS
The method is suitable for blood EtG analysis.
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China.
Bao-Hua
BH
Shen
Hui
H
Yan
Hui
H
Zhao
Xian-Yi
XY
Zhuo
D000428:Alcohol Drinking / Q000097:blood; D000437:Alcoholism / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D053593:Forensic Toxicology / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005965:Glucuronates / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015415:Biomarkers; D005965:Glucuronates; C093924:ethyl glucuronide