Altered circadian rhythm of the clock genes in fibrotic livers induced by carbon tetrachloride.
Disruption in circadian rhythms either by mutation in mice or by shiftwork in people, is associated with an increased risk for the development of multiple organ diseases. In turn, organ disease may influence the function of clock genes and peripheral circadian systems. Here we showed that hepatic fibrosis induced by carbon tetrachloride in mice leads to alterations in the circadian rhythms of hepatic clock genes. Especially, we found an impaired daily Cry2 rhythm in the fibrotic livers, with markedly decreased levels during the day time while compared with control livers. Associatively, the expressions of two important clock-regulated genes peroxisome proliferator-activated receptor alpha and cytochrome P450 oxidoreductase lost circadian rhythm with significantly decreased levels during the light-dark (12/12h) cycle in fibrotic livers.
Center for Molecular Metabolism, Nanjing University of Science and Technology, Nanjing, China.
Peng
P
Chen
Xiamusiya
X
Kakan
Jianfa
J
Zhang
D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology*; D002940:Circadian Rhythm / Q000187:drug effects* / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D055166:Hepatic Stellate Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced* / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009251:NADPH-Ferrihemoprotein Reductase / Q000235:genetics; D047493:PPAR alpha / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D047493:PPAR alpha; D012333:RNA, Messenger; D002251:Carbon Tetrachloride; D009251:NADPH-Ferrihemoprotein Reductase