Qi-Shao-Shuang-Gan, a combination of Astragalus membranaceus saponins with Paeonia lactiflora glycosides, ameliorates polymicrobial sepsis induced by cecal ligation and puncture in mice.
The present study was performed to investigate the anti-septic effects of Qi-Shao-Shuang-Gan (QSSG), a combination of Astragalus membranaceus saponins (SAM) and Paeonia lactiflora glycosides (GPL), in septic mice induced by cecal ligation and puncture. QSSG was shown to elevate the survival rate of mice, decrease infiltration of polymorphonuclear leukocytes into livers and lungs, lower serum levels of myeloperoxidase, nitric oxide, and lactate dehydrogenase, and decrease mRNA expressions of inducible nitric oxide synthase and interleukin-1β in livers. It also restored the impaired expressions of protein C (PC) mRNA in mouse livers and expressions of thrombomodulin and endothelial PC receptor mRNA in endothelial cells. Neither SAM nor GPL alone could significantly increase the survival rate of septic mice. The findings indicate that QSSG exerts protective action against polymicrobial sepsis by inhibiting systemic inflammatory response and upregulating PC pathway, and there are synergistic effects between SAM and GPL.
Gao
Xing-hua
XH
Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang Road, Nanjing 210009, China.
Xu
Xian-xiang
XX
Pan
Rong
R
Wang
Chong
C
Sheng
Rong
R
Xia
Yu-feng
YF
Dai
Yue
Y
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D027885:Astragalus propinquus / Q000737:chemistry; D001779:Blood Coagulation Factors / Q000235:genetics / Q000378:metabolism; D002432:Cecum / Q000601:surgery; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006027:Glycosides / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D020556:Neutrophil Infiltration / Q000187:drug effects; D009569:Nitric Oxide / Q000097:blood; D019001:Nitric Oxide Synthase / Q000235:genetics; D029596:Paeonia / Q000737:chemistry; D009195:Peroxidase / Q000097:blood; D011486:Protein C / Q000235:genetics / Q000378:metabolism; D011654:Pulmonary Edema / Q000517:prevention & control; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D012503:Saponins / Q000627:therapeutic use*; D018805:Sepsis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D018180:Thrombomodulin / Q000235:genetics / Q000378:metabolism
D001779:Blood Coagulation Factors; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D053583:Interleukin-1beta; D011486:Protein C; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D012503:Saponins; D018180:Thrombomodulin; C091963:activated protein C receptor; D009569:Nitric Oxide; D007770:L-Lactate Dehydrogenase; D009195:Peroxidase; D019001:Nitric Oxide Synthase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase