Ultrasensitive detection of non-amplified genomic DNA by nanoparticle-enhanced surface plasmon resonance imaging.
Technologies today available for the DNA detection rely on a combination of labeled probes hybridized to target sequences which are amplified by polymerase chain reaction (PCR). Direct detection methods that eliminate the requirement for both PCR and labeling steps could afford faster, cheaper and simpler devices for the analysis of small amounts of unamplified DNA. In this work we describe the results obtained in the ultrasensitive detection of non-amplified genomic DNA. We analyzed certified reference materials containing different amounts of genetically modified DNA by using a detection method which combines the nanoparticle-enhanced surface plasmon resonance imaging (SPRI) biosensing to the peptide nucleic acids (PNAs) improved selectivity and sensitivity in targeting complementary DNA sequences. The method allowed us to obtain a 41 zM sensitivity in targeting genomic DNA even in the presence of a large excess of non-complementary DNA.
Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, I-95125 Catania, Italy.
Roberta
R
D'Agata
Roberto
R
Corradini
Cristina
C
Ferretti
Laura
L
Zanoli
Marcello
M
Gatti
Rosangela
R
Marchelli
Giuseppe
G
Spoto
D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D004274:DNA, Recombinant / Q000032:analysis / Q000235:genetics; D006046:Gold; D053768:Metal Nanoparticles; D020135:Peptide Nucleic Acids; D030821:Plants, Genetically Modified / Q000235:genetics; D013025:Glycine max / Q000235:genetics; D020349:Surface Plasmon Resonance / Q000379:methods* / Q000706:statistics & numerical data
D018744:DNA, Plant; D004274:DNA, Recombinant; D020135:Peptide Nucleic Acids; D006046:Gold; D004247:DNA