Phylogenetic and biological characterization of Newcastle disease virus isolates from Pakistan.
Eight Newcastle disease virus isolates from Pakistan were sequenced and characterized. A PCR matrix gene assay, designed to detect all avian paramyxovirus 1, did not detect four of the isolates. A new matrix gene test that detected all isolates was developed. Phylogenetic analysis and pathotyping confirmed that virulent viruses of different genotypes are circulating in Pakistan.
USDA-ARS, Southeast Poultry Research Laboratory, 934 College Station Rd., Athens, GA 30605, USA.
Taseer Ahmed
TA
Khan
Cary A
CA
Rue
Shafqat Fatima
SF
Rehmani
Ayaz
A
Ahmed
Jamie L
JL
Wasilenko
Patti J
PJ
Miller
Claudio L
CL
Afonso
D000818:Animals; D002645:Chickens; D016000:Cluster Analysis; D005838:Genotype; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000453:epidemiology* / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D010154:Pakistan / Q000453:epidemiology; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology* / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D012367:RNA, Viral