A visible dye-based staining method for DNA in polyacrylamide gels by ethyl violet.
We describe a visible dye-based staining method for DNA in polyacrylamide gels using ethyl violet (EV). The novel method is a background-free, sensitive, economical, and simple procedure involving only staining and washing steps that can be completed within 30 min. As little as 0.8-1.6 ng of phiX174 DNA/HaeIII can be detected by EV, which is about eightfold more sensitive than Nile blue (NB) stain and twofold less sensitive than ethidium bromide (EB) stain.
Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou, Zhejiang, China.
Wei-Tao
WT
Cong
Zhong-Xin
ZX
Zhu
Hong-Zhang
HZ
He
Yan
Y
Jin
Cheng-Xi
CX
Jiang
Jung-Kap
JK
Choi
Li-Tai
LT
Jin
Xiao-Kun
XK
Li
D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D012394:Rosaniline Dyes; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000191:economics / Q000379:methods*; D014764:Viral Proteins / Q000235:genetics
C029555:E protein, bacteriophage X174; D012394:Rosaniline Dyes; D014764:Viral Proteins; D004247:DNA; C034887:ethyl violet