Hybrid Root SEAL (MetaSEAL) creates hybrid layers in radicular dentin only when EDTA is used as the final rinse.
OBJECTIVE
To test if the hybrid layer formation by Hybrid Root SEAL (Sun Medical Co.), a 4-META-containing auto-adhesive self-etching root canal sealer, is affected by the sequence of irrigants employed for removing canal wall smear layers during root canal treatment.


METHODS
Single-rooted teeth were shaped and irrigated with EDTA as initial rinse/NaOCl as active final rinse (Group 1), or NaOCl as initial rinse/EDTA as active final rinse (Group 2). All canals were obturated with Hybrid Root SEAL using a single-cone technique. Root slices derived from the coronal, middle and apical thirds of the roots were processed for transmission electron microscopy after removing the gutta-percha, leaving the sealer intact. Additional filled canals from the two groups were evaluated for fluid leakage.


RESULTS
Hybrid layer was absent in Group 1 and was present only when a collagen matrix was produced by EDTA demineralization (Group 2). Significantly more leakage (4.03 +/- 1.94 microL min(-1) vs. 1.50 +/- 0.42 microL min(-1); P < 0.05) was observed in the absence of dentin hybridization.
Pinna
Luigi
L
Department of Conservative Dentistry, University of Cagliari, Sardinia, Italy.
Loushine
Robert J
RJ
Bishop
Frank D
FD
Cotti
Elisabetta
E
Weller
R Norman
RN
Pashley
David H
DH
Tay
Franklin R
FR
D002614:Chelating Agents / Q000627:therapeutic use*; D003094:Collagen / Q000648:ultrastructure; D003763:Dental Leakage / Q000145:classification; D003786:Dental Pulp Cavity / Q000648:ultrastructure*; D003804:Dentin / Q000648:ultrastructure*; D004492:Edetic Acid / Q000627:therapeutic use*; D006171:Gutta-Percha / Q000627:therapeutic use; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D019279:Resin Cements / Q000737:chemistry; D012387:Root Canal Filling Materials / Q000737:chemistry*; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D018915:Root Canal Preparation / Q000379:methods; D016369:Smear Layer; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D013499:Surface Properties
D002614:Chelating Agents; D008689:Methacrylates; D019279:Resin Cements; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; C534239:metaseal; C034139:4-methacryloxyethyltrimellitic acid anhydride; D006171:Gutta-Percha; D003094:Collagen; D004492:Edetic Acid; D012973:Sodium Hypochlorite