Quantification of growth hormone in serum by isotope dilution mass spectrometry.
Interassay variation of antibody-based routine tests hampers comparability of measurement results for growth hormone (GH) between different laboratories and decision making in clinical practice. Here it is demonstrated that quantification of GH by isotope dilution mass spectrometry (IDMS) constitutes a way to obtain precise and reliable results that can be referred to in evaluation of performance of commercial test kits. With the IDMS method developed, tryptic cleavage products YSFLQNPQTSLCFSESIPTPSNR (T6) and LEDGSPR (T12) of GH are quantified by liquid chromatography tandem mass spectrometry (LC-MS/MS) using isotopically labeled forms of the peptides as internal standards. The GH cleavage fragments are obtained by whole serum tryptic proteolysis and then extracted from the resulting mixture by semipreparative reversed-phase LC followed by strong cation exchange chromatography. Analysis of blank serum spiked with recombinant 22-kDa GH at different concentration levels would result in a mean recovery of 101.6%, a standard deviation (SD) of 2.5%, a combined uncertainty (u(c)) of 3.0%, and a limit of quantification (LOQ) of 1.7 microg/L when quantifying T6 as a GH-derived fragment, whereas recovery=100.7%, SD=2.4%, u(c)=2.5%, and LOQ=2.7 microg/L were found with T12. The potential to acquisition of reference values is exemplified by application to serum materials used in a recent quality assessment exercise for routine laboratories.
Physikalisch-Technische Bundesanstalt (PTB), 38116 Braunschweig, Germany. christian.arsene@ptb.de
Cristian G
CG
Arsene
André
A
Henrion
Nina
N
Diekmann
Jenny
J
Manolopoulou
Martin
M
Bidlingmaier
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D013006:Growth Hormone / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007201:Indicator Dilution Techniques; D007554:Isotopes / Q000032:analysis; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D002352:Carrier Proteins; D007554:Isotopes; D011994:Recombinant Proteins; D013006:Growth Hormone; C051815:somatotropin-binding protein