Genetic variation among lambs in peripheral IgE activity against the larval stages of Teladorsagia circumcincta.
IgA and IgE activity against Teladorsagia circumcincta was investigated in a flock of Texel lambs following natural, mixed nematode infection among lambs. The distribution of IgA activity was similar to a gamma distribution whereas IgE activity was different. Box-Cox analysis demonstrated that X0.25 was a suitable transformation to normalise IgE responses. The transformed IgE activity was under moderate to strong genetic control. Nine different allergens were identified by proteomic analysis. Tropomyosin was selected for further analysis. IgE activity against tropomyosin was moderately heritable and associated with decreased egg counts and with reduced body weight at the time of sampling.
Glasgow University Veterinary School, Bearsden Road, Glasgow G61 1QH, UK.
L
L
Murphy
P D
PD
Eckersall
S C
SC
Bishop
J J
JJ
Pettit
J F
JF
Huntley
R
R
Burchmore
M J
MJ
Stear
D000485:Allergens / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001835:Body Weight; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005260:Female; D014644:Genetic Variation; D007073:Immunoglobulin E / Q000097:blood*; D007814:Larva / Q000254:growth & development / Q000276:immunology; D008297:Male; D010270:Parasite Egg Count; D040901:Proteomics; D012756:Sheep / Q000469:parasitology; D012757:Sheep Diseases / Q000235:genetics / Q000276:immunology / Q000469:parasitology*; D014251:Trichostrongyloidea / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D014252:Trichostrongyloidiasis / Q000235:genetics / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D014335:Tropomyosin / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D000485:Allergens; D000947:Antigens, Helminth; D014335:Tropomyosin; D007073:Immunoglobulin E