[Inhibitive effect of human foamy virus induced IL-24 on cancer cells].
OBJECTIVE
To construct replication-defective HFV-IL24 virus vector and to investigate its inhibitive effect on cancer cells after infected or transfected by this vector.


METHODS
pDeltaphi-IL24 was constructed and was co-transfected with helper-plasmids into HEK 293T cells. Recombinant HFV-IL24 vector was extracted to infect HeLa cells and the inhibitive effect of IL-24 on cancer cells was examined by RT-PCR, MTT, cell counting and FCM methods.


RESULTS
pDeltaphi-IL24 plasmid and HFV-IL24 particles were successfully constructed. HeLa cells exhibited obvious growth inhibition, cell cycle variation and visible apoptosis (mortality) after infected or transfected by HFV-IL24 or pDeltaphi-IL24.


CONCLUSIONS
The results of the study provide some evidence for human foamy virus used as an effective gene transfer tool and for cancer gene therapy with HFV-IL24.
College of Life Science, Medical College, Wuhan University, Wuhan, China. mcs188@163.com
Si-chong
SC
Chen
Chen
C
Yao
Fang
F
Chen
Wan-hong
WH
Liu
Wei-ping
WP
Tao
Wen-xin
WX
Li
D002453:Cell Cycle; D049109:Cell Proliferation; D002470:Cell Survival; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D009369:Neoplasms / Q000473:pathology / Q000628:therapy*; D010957:Plasmids; D053150:Simian foamy virus / Q000235:genetics*; D000099180:Interleukin-24
D007378:Interleukins; D000099180:Interleukin-24