[Development and application of X-chromosomal STR multiplex amplification system].
OBJECTIVE
To explore the application of X-chromosomal STR(X-STR) for forensic identification and paternity testing.


METHODS
Six X-STR loci DXS6801, DXS9902, DXS6809, DXS6803, DXS6804 and DXS6799 were amplified in a single PCR reaction. PCR products were analyzed using capillary electrophoresis and 3100 Genetic Analyzer and GeneMapper ID v3.1 Analysis Software.


RESULTS
The alleles of all six X-STR loci were successfully obtained with unambiguous genotyping, high sensitivity and reproducibility.


CONCLUSIONS
The multiplex PCR of six X-STR loci is useful in forensic identification, particularly for sisters cases.
Department of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China. liuql@mail.sysu.edu.cn
Qiu-Ling
QL
Liu
De-Jian
DJ
Lü
Hu
H
Zhao
D000483:Alleles; D041321:Chromosomes, Human, X / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D005453:Fluorescence; D053803:Forensic Genetics / Q000379:methods; D005784:Gene Amplification; D005787:Gene Frequency; D056426:Genetic Loci / Q000235:genetics; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D017931:DNA Primers