[X-sNP genotyping using the TaqMan probe technology].
OBJECTIVE
To develop a rapid, accurate and economical real time fluorescence PCR method with TaqMan probe technology to detect the X chromosome single nucleotide polymorphism (X-SNP).


METHODS
TaqMan probes and polymerase chain reaction primers were respectively designed according to the 13 X-SNP. Then, the X-SNP were genotyped after the amplification by real time fluorescence PCR.


RESULTS
All the loci follow the Hardy-Weinberg equilibrium. The polymorphic information content for 13 distinct loci varied between 0.3497 and 0.3750 while the heterozygosity ranged from 0.4537 to 0.5021. A real time fluorescent PCR method based on TaqMan probe was successfully developed and the results were accordant with those analyzed by DNA sequencing of the 13 X-SNP.


CONCLUSIONS
The allele specific real time fluorescence PCR based on TaqMan probe is a sensitive, simple technology and suitable for rapid analysis of XSNP. All the loci show highly polymorphic and may be potential in forensic genetics.
Zhang
Su-hua
SH
Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China. zsh-daisy@163.com
Li
Li
L
Li
Cheng-tao
CT
Zhao
Shu-min
SM
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D041321:Chromosomes, Human, X / Q000235:genetics*; D004247:DNA / Q000097:blood / Q000235:genetics; D015342:DNA Probes; D005260:Female; D005456:Fluorescent Dyes; D053803:Forensic Genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase
D015342:DNA Probes; D005456:Fluorescent Dyes; D004247:DNA; D019914:Taq Polymerase