A visible dye-based staining method for DNA in polyacrylamide gels by ethyl violet.
We describe a visible dye-based staining method for DNA in polyacrylamide gels using ethyl violet (EV). The novel method is a background-free, sensitive, economical, and simple procedure involving only staining and washing steps that can be completed within 30 min. As little as 0.8-1.6 ng of phiX174 DNA/HaeIII can be detected by EV, which is about eightfold more sensitive than Nile blue (NB) stain and twofold less sensitive than ethidium bromide (EB) stain.
Cong
Wei-Tao
WT
Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou, Zhejiang, China.
Zhu
Zhong-Xin
ZX
He
Hong-Zhang
HZ
Jin
Yan
Y
Jiang
Cheng-Xi
CX
Choi
Jung-Kap
JK
Jin
Li-Tai
LT
Li
Xiao-Kun
XK
D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D012394:Rosaniline Dyes; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000191:economics / Q000379:methods*; D014764:Viral Proteins / Q000235:genetics
C029555:E protein, bacteriophage X174; D012394:Rosaniline Dyes; D014764:Viral Proteins; D004247:DNA; C034887:ethyl violet