The relationship between plasma and placental tissue factor, and tissue factor pathway inhibitors in severe pre-eclampsia patients.
OBJECTIVE
To investigate the mechanism underlying the hypercoagulable state in severe pre-eclampsia.


METHODS
Plasma tissue factor (TF) and tissue factor pathway inhibitor (TFPI) expression from pre-eclampsia patients and healthy pregnant controls were determined by ELISA. Placental TF and TFPI gene and protein expression were detected by quantitative RT-PCR, immunohistochemistry, and Western analysis.


RESULTS
The plasma TF level in the pre-eclampsia group was significantly higher than the control group (p<0.01), and surprisingly, the plasma TFPI-1 and TFPI-2 in the pre-eclampsia group were significantly lower (p<0.01). Placental TF gene and protein expression levels in the pre-eclampsia group was significantly higher than the control group, while TFPI-1 and TFPI-2 levels were significantly lower (p<0.05). Lastly, a significant correlation was found between plasma and placental TF protein levels in the pre-eclampsia group (p<0.01).


CONCLUSIONS
Higher expression and/or release of TF from the placenta may contribute towards a pathological hypercoagulable state in pre-eclampsia patients.
Department of Obstetrics and Gynecology, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, 200233 China. teng1008@sohu.com
Yincheng
Y
Teng
Rongzhen
R
Jiang
Qide
Q
Lin
Chuanwei
C
Ding
Zuo
Z
Ye
D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006023:Glycoproteins; D006801:Humans; D007150:Immunohistochemistry; D008074:Lipoproteins / Q000032:analysis* / Q000097:blood*; D010920:Placenta / Q000737:chemistry / Q000378:metabolism; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013925:Thromboplastin / Q000032:analysis* / Q000378:metabolism*; D016896:Treatment Outcome
D006023:Glycoproteins; D008074:Lipoproteins; C051928:lipoprotein-associated coagulation inhibitor; C021240:tissue-factor-pathway inhibitor 2; D013925:Thromboplastin