[Preparation of polyclonal antibody against human Fkbp19].
OBJECTIVE
To prepare the polyclonal antibody against human Fkbp19 for future functional studies of Fkbp19.


METHODS
Prokaryotic expression vector pET21a-Fkbp19 was transformed into E.coli BL21-DE3, and then induced by IPTG. The recombinant protein was purified by Ni-NTA resin and used to immunize rabbits. Antiserums were used for Western blot and immunofluorescence to detect Fkbp19 in breast tumor cell.


RESULTS
His-Fkbp19 fusion protein was successfully expressed in E.coli. High titer polyclonal antiserum was obtained by immunizing rabbits with purified His-Fkbp19 fusion protein. The antiserum could detect endogenous Fkbp19 protein in breast tumor cell very well by Western blot.


CONCLUSIONS
The polyclonal antiserum against Fkbp19 was successfully generated.
Fan
Kai-ji
KJ
School of Life Science, Lanzhou University, Lanzhou, China.
Zhang
Yan
Y
Liu
Nai-fa
NF
Sun
Qiang
Q
Yang
Xiao
X
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008297:Male; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D022021:Tacrolimus Binding Proteins / Q000235:genetics / Q000276:immunology*
D000906:Antibodies; C511900:FKBP11 protein, human; D011994:Recombinant Proteins; D022021:Tacrolimus Binding Proteins