A combination method for preparing casting and transparent liver specimen.
With the development of hepatic surgery and radiology, a more accurate understanding of intrahepatic vessels and hepatic segments is necessary. Previously, research in these fields was primarily by means of dissecting livers, preparing corrosion cast specimens, reconstructing three-dimensional MSCT images of intrahepatic vessels, and so on. The aim of this study was to search for a new specimen preparing method, which could demonstrate intrahepatic vessels and the relationships between internal vessels and external structures of liver simultaneously. Rabbit livers were prepared with three different techniques in this study: (a) corrosion-cast technique; (b) transparency technique; and (c) combination technique of cast and transparency. The results showed that the combination of casting and transparent liver specimens in group C could demonstrate both the intrahepatic vessels and the external structures clearly. The relationships between the internal vessels and external structures could also be observed clearly. In conclusion, the combination method for preparing casting and transparent liver specimen created a new approach for the research of intrahepatic vessels, especially for applied basic research of hepatic segments.
Department of Anatomy, Dalian Medical University, Liaoning, China.
Nan
N
Zheng
Jin
J
Gong
Sheng-Bo
SB
Yu
Jian-Fei
JF
Zhang
Wei
W
Tang
Cheng-Hong
CH
Zhang
Jian
J
Bai
Jie
J
Liu
Hong-Jin
HJ
Sui
Bing-Yi
BY
Shi
D000818:Animals; D016319:Corrosion Casting / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008099:Liver / Q000033:anatomy & histology*; D011817:Rabbits; D013048:Specimen Handling