The effect of long-term DHEA treatment on glucose metabolism, hydrogen peroxide and thioredoxin levels in the skeletal muscle of diabetic rats.
Dehydroepiandrosterone (DHEA) is an endogenous steroid hormone involved in a number of biological actions. This study shows the effects of DHEA on glucose metabolism, hydrogen peroxide and thioredoxin levels in the skeletal muscle of control and diabetic rats. Control and diabetic rats were chronically treated with DHEA (10mg/kg) diluted in oil. Plasma concentration of DHEA and glucose, glucose uptake and oxidation, hydrogen peroxide, GLUT4, Akt and thioredoxin (Trx) was measured in the muscle. Results showed that there was a decrease in blood glucose in diabetic rats, probably linked to an increase in the glucose oxidation by the muscle or glucose uptake by some tissues. Despite the increase in the expression of GLUT4 in DHEA-treated rats, the glucose uptake was only higher in the control rats, showing that the glucose transporter may be present but not functional in the diabetic rats. The low expression of Trx due to diabetes became even lower with DHEA treatment. Although the reduction in blood glucose may be favorable, the decrease in Akt and Trx displays an environment conducive to redox imbalance. Thus, further studies are needed to ascertain the effects of DHEA treatment in diabetic rats.
Jahn
Matheus Parmegiani
MP
Laboratório de Metabolismo e Endocrinologia Comparada, Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Av. Sarmento Leite, 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil. matheusjahn@hotmail.com <matheusjahn@hotmail.com>
Jacob
Maria Helena Vianna Metello
MH
Gomes
Luana Ferreira
LF
Duarte
Roxane
R
Araújo
Alex Sander da Rosa
AS
Belló-Klein
Adriane
A
Ribeiro
Maria Flávia Marques
MF
Kucharski
Luiz Carlos
LC
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects*; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013879:Thioredoxins / Q000378:metabolism*; D013997:Time Factors
D001786:Blood Glucose; D003687:Dehydroepiandrosterone; D013879:Thioredoxins; D006861:Hydrogen Peroxide; D005947:Glucose