Nocardiopsis terrae sp. nov., a halophilic actinomycete isolated from saline soil.
A Gram-positive, moderately halophilic, facultatively alkaliphilic, catalase- and oxidase-positive, obligately aerobic, filamentous actinomycete strain, designated YIM 90022(T), was isolated from saline soil collected from the Qaidam Basin, north-west China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate was a member of the genus Nocardiopsis and the sequence similarities between the isolate and the type strains of members of the genus Nocardiopsis were in the range of 95.1-98.7%. Phenotypic and chemotaxonomic properties of this organism also indicated that strain YIM 90022(T) was a member of the genus Nocardiopsis. The strain grew well on most of the media tested, producing yellow-white to deep brown substrate mycelium and white aerial mycelium. Light gray to deep brown diffusible pigments were produced. The substrate mycelium was well developed and fragmented with age; the aerial mycelium produced long, straight to flexuous spore chains with non-motile, smooth-surfaced, rod-shaped spores on them. The strain grew in the presence of 1-15% (w/v) total salts (optimum, 3-5%) and at pH 6.0-10.5 (optimum, pH 8.5) and 10-45 degrees C (optimum, 30 degrees C). Whole-cell hydrolysates of strain YIM 90022(T) contained meso-diaminopimelic acid and no diagnostic sugars. The predominant menaquinones were MK-10(H(4)), MK-9(H(8)), MK-10(H(6)) and MK-10(H(8)). Polar lipids comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylmethylethanolamine. The major cellular fatty acids were iso-C(16:0), anteiso-C(17:0), 10-methyl-C(18:0) and 10-methyl-C(17:0). The DNA G + C content of strain YIM 90022(T) was 71.5 mol%. The combination of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data supported the suggestion that strain YIM 90022(T) represents a new species of the genus Nocardiopsis, for which the name Nocardiopsis terrae sp. nov. is proposed. The type strain is YIM 90022(T) (=CCTCC AA 208011(T) =KCTC 19431(T)).
Chen
Yi-Guang
YG
The Key Laboratory for Microbial Resources of the Ministry of Education, Kunming, People's Republic of China. mchenjsu@yahoo.com.cn
Zhang
Yu-Qin
YQ
Tang
Shu-Kun
SK
Liu
Zhu-Xiang
ZX
Xu
Li-Hua
LH
Zhang
Li-Xin
LX
Li
Wen-Jun
WJ
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S