Effect of 103Pd radioactive stent on caspase-9, cholangiocarcinoma cell growth and its radiosensitivity.
BACKGROUND
To investigate the effect of (103)Pd radioactive stent on Caspase-9, cholangiocarcinoma cell growth and its radiosensitivity.


METHODS
Cholangiocarcinoma was treated with (103)Pd radioactive stent at different period. Radiosensitivity of the cells was detected by methyl thiazolyl tetrazolium (MTT) method. Apoptosis of cholangiocarcinoma cells was detected by immunohistochemistry and electron microscope. The activity of Caspase-9 was detected by non-radioimmunoprecipitation, while its protein expression was detected by Western blot.


RESULTS
(103)Pd radioactive stent had significant inhibitive effect on cholangiocarcinoma cells and it could induce apoptosis. After treatment by (103)Pd radioactive stent for 10 days, the activity of Caspase-9 was gradually enhanced, which was markedly decreased in common stent group. Cholangiocarcinoma cells had relatively high sensitivity to (103)Pd radiation.


CONCLUSIONS
(103)Pd radioactive stent can activate caspase-9 gene to induce apoptosis of cholangiocarcinoma cell, inhibit its growth and enhance its radiosensitivity.
Department of General Surgery, Sheng jing hospital, China Medical University, Shenyang 110004, China.
He
H
Guijin
Guo
G
QiYong
Zhao
Z
XiaoDan
Ji
J
DaWei
Gu
G
Xi
Pan
P
ChunLai
Wang
W
Liu
Dai
D
XianWei
D017209:Apoptosis / Q000528:radiation effects; D001650:Bile Duct Neoplasms / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D053453:Caspase 9 / Q000378:metabolism*; D049109:Cell Proliferation / Q000528:radiation effects*; D018281:Cholangiocarcinoma / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D006801:Humans; D010165:Palladium / Q000008:administration & dosage*; D015607:Stents
D010165:Palladium; D053453:Caspase 9