The prevalence of plasmid-mediated quinolone resistance determinants among clinical isolates of ESBL or AmpC-producing Escherichia coli from Chinese pediatric patients.
Three kinds of PMQR determinants (qnr genes, aac(6')-Ib-cr, and qepA) have been discovered and shown to be widely distributed among clinical isolates. To characterize the prevalence of PMQR determinants in ESBL or AmpC-producing E. coli clinical isolates in Chinese children, a total of 292 ESBL or AmpC-producing E. coli clinical isolates collected from five children's hospitals in China from 2005 to 2006 were screened for PMQR determinants by PCR. Twenty (6.8%) of the 292 isolates were positive for PMQR determinants. A total of 12 (4.1%) isolates were positive for qnr genes, comprising three positive for qnrA (1.0%), three for qnrB (1.0%), and six for qnrS (2.1%). Twenty-four (8.2%) isolates were positive for aac(6')-Ib, of which 10 (3.4% of 292) had the -cr variant. There was no qepA gene detected in the isolates. Conjugation revealed that qnr, aac(6')-Ib-cr, and ESBL-encoding genes were transferred together.
Beijing Children's Hospital, Affiliated to Capital Medical University, Beijing, 100045, China.
Chenxi
C
Han
Yonghong
Y
Yang
Minggui
M
Wang
Aihua
A
Wang
Quan
Q
Lu
Xiwei
X
Xu
Chuanqing
C
Wang
Lan
L
Liu
Qiulian
Q
Deng
Xuzhuang
X
Shen
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003227:Conjugation, Genetic; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010957:Plasmids / Q000235:genetics; D015363:Quinolones / Q000494:pharmacology*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D015363:Quinolones; C048795:AmpC beta-lactamases; D001618:beta-Lactamases