[Development of SNP multiplexes utilizing universal reporter primers for forensic purposes].
OBJECTIVE
To establish a simple, fast and economical technique for multiplex-typing SNPs and to explore its potential forensic application.


METHODS
Five Y-SNP loci (IMS-JST164520, IMS-JST021354, IMS-JST003305, M119 and M134) were selected and the allele specific primers of each locus were designed with the universal reporter primers tailed at their 5' end. Alleles of these loci were amplified first by allele specific primers, then amplified by universal reporter primers tagged by fluorescent dye.


RESULTS
A fluorescent-multiplex PCR system of the five Y-SNP loci was established. The typing results showed that two different colors of product peaks denoted two different alleles of a SNP locus, and the fragment sizes of alleles among different SNP loci were different. The haplotype diversity of these five loci was estimated to be 0.8655 in Wuhan Han population.


CONCLUSIONS
The multiplex-typing SNPs based on the universal reporter primers is a simple, fast, and economical technique, and may have good application value in forensic medicine.
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. healytomato@163.com
Bai-fang
BF
He
Yin-bao
YB
Wu
Jin
J
Li
Hui
H
Chen
Qing-en
QE
Yang
Dai-xin
DX
Huang
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D041322:Chromosomes, Human, Y / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D053803:Forensic Genetics; D005787:Gene Frequency; D005819:Genetic Markers; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers