Association of GSTT1, GSTM1 and CYP1A1 polymorphisms with susceptibility to systemic lupus erythematosus in the Chinese population.
BACKGROUND
GSTT1, GSTM1, CYP1A1 are enzymes responsible for the detoxification of the toxicant which may be involved in the development of systemic lupus erythematosus (SLE). We examined the relationship between the risk of SLE and the polymorphisms of these genes in the Chinese population.


METHODS
Samples from 298 SLE patients and 284 healthy controls were collected. Polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotypes of CYP1A1 m2 and m4, while multiplex PCR was used to analyze the genotypes of GSTT1 and GSTM1.


RESULTS
Statistically significant difference was observed in genotypes for GSTM1 (p=0.003, OR 1.66 [95% CI 1.19-2.32]), but not for GSTT1 (p=0.119, OR 0.77 [95% CI 0.56-1.07]), in the SLE patients as compared with the controls. Combinational analysis for double-null deletion of both GSTT1 and GSTM1 showed no significant difference (p=0.863, OR 1.03 [95% CI 0.70-1.52]). Significant difference was observed in the genotype frequencies (p=0.013), but not in the allele frequencies (p=0.444, OR 0.90 [95% CI 0.70-1.17]), of CYP1A1 m2. All candidates have a wild-type genotype for CYP1A1 m4.


CONCLUSIONS
Polymorphisms of GSTM1 are associated with SLE in the Chinese population.
Zhang
Jufeng
J
Biomedical Research Institute, Shenzhen-PKU-HKUST Medical Center, Shenzhen, 518036, China.
Deng
Jingui
J
Zhang
Chao
C
Lu
Yanxin
Y
Liu
Li
L
Wu
Qi
Q
Shao
Yong
Y
Zhang
Jie
J
Yang
Hong
H
Yu
Bo
B
Wan
Jun
J
D044466:Asian People / Q000235:genetics*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D055106:Genome-Wide Association Study / Q000379:methods; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000201:enzymology / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics
D019363:Cytochrome P-450 CYP1A1; D005819:Genetic Markers; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1