Fluorescence quenched quinone methide based activity probes--a cautionary tale.
A carbamate linked quenching group coupled with a pro-quinone methide reactive core provides an effective tool for studying enzyme function without problems associated with background fluorescence from unreacted probe. However, the relatively slow fragmentation of the carbamate linkage in such a strategy may cause problems of loss of signal or a decoupling of enzyme activity and labelling.
Sellars
Jonathan D
JD
Centre for Bioactive Chemistry, Department of Chemistry, University of Durham, Sciences Laboratories, South Road, Durham, UK DH1 3LE.
Landrum
Marie
M
Congreve
Aileen
A
Dixon
David P
DP
Mosely
Jackie A
JA
Beeby
Andrew
A
Edwards
Robert
R
Steel
Patrick G
PG
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D002219:Carbamates / Q000737:chemistry*; D043182:Carboxylesterase / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D045563:Indolequinones / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D002219:Carbamates; D005456:Fluorescent Dyes; D045563:Indolequinones; C068040:quinone methide; D043182:Carboxylesterase