Development and characterization of a congenic strain carrying Pbwg12, a growth QTL on mouse chromosome 12.
In previous genome-wide QTL studies, Pbwg12 on mouse chromosome 12 was discovered in a gene pool of wild Mus musculus castaneus mice. Pbwg12 does not have a main effect but has an epistatic interaction effect on body weight after birth. In this study, we developed a congenic strain, named B6.Cg-Pbwg12, with an approximately 59-Mb wild-derived genomic region harboring Pbwg12, by recurrent backcrossing to C57BL/6J. A phenotypic comparison between B6.Cg-Pbwg12 and C57BL/6J revealed that Pbwg12 does not have any main effects on body weight at 1-10 weeks of age but has a main effect on body weight gain at 6-10 weeks. A new QTL with a male-specific effect on kidney weight was discovered within the introgressed region.
Laboratory of Animal Genetics, Division of Applied Genetics and Physiology, Graduate School of Bioagricultural Sciences, Nagoya University, Aichi, Japan.
Akira
A
Ishikawa
Chun
C
Li
D000818:Animals; D001835:Body Weight; D007178:Inbreeding; D007668:Kidney / Q000033:anatomy & histology; D008297:Male; D051379:Mice; D020297:Mice, Congenic / Q000235:genetics*; D008810:Mice, Inbred C57BL; D009929:Organ Size