Rapid visualization and large-scale profiling of bacterial lipoproteins with chemical reporters.
Lipoproteins are a largely uncharacterized class of proteins in bacteria. In this study, metabolic labeling of bacteria with fatty acid chemical reporters allowed rapid profiling of lipid-modified proteins. We identified many candidate lipoproteins in Escherichia coli and detected a novel modification on YjgF. This chemical approach should facilitate future characterization of lipoproteins.
Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065, USA.
Kavita J
KJ
Rangan
Yu-Ying
YY
Yang
Guillaume
G
Charron
Howard C
HC
Hang
D000480:Alkynes / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry*; D008074:Lipoproteins / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure
D000480:Alkynes; D029968:Escherichia coli Proteins; D005227:Fatty Acids; D008074:Lipoproteins