Interleukin 10 receptor alpha subunit (IL-10RA) gene polymorphism and IL-10 serum levels in Egyptian atopic patients.
OBJECTIVE
To assess the value of serum interleukin (IL) 10 levels as an immunological marker in atopy and to determine the role of an IL-10RA gene single nucleotide polymorphism (SNP) (serine 138-to-glycine exchange [S138G]) in the pathogenesis of atopic diseases.


METHODS
Seventy-five patients with atopic disorders were compared with 25 age-matched healthy volunteers. Serum total immunoglobulin (Ig) E and IL-10 levels were measured by enzyme-linked immunosorbent assay and the IL-10RA gene S138G variant was screened by multiplex allele-specific polymerase chain reaction.


RESULTS
There was a significant association between G allele frequencies of the S138G variant (62%, 60% and 68% for atopic asthma, atopic dermatitis, and allergic rhinitis, respectively) in atopic patients compared to in controls. There were significant differences in mean IgE levels but not mean serum IL-10 levels between the allelic variants in atopy groups.


CONCLUSIONS
The IL-10RA gene SNP S138G may contribute to susceptibility to atopic diseases but serum IL-10 level is not a sensitive indicator in atopy.
Hussein
P Y M
PY
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. yousrihussein@hotmail.com
Zahran
F
F
Ashour Wahba
A
A
Ahmad
A S
AS
Ibrahiem
M M
MM
Shalaby
S M
SM
El Tarhouny
S A
SA
El Sherbiny
H M
HM
Bakr
N
N
D004534:Egypt; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics* / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D016753:Interleukin-10 / Q000097:blood*; D020641:Polymorphism, Single Nucleotide; D021122:Protein Subunits; D053701:Receptors, Interleukin-10 / Q000235:genetics*
D021122:Protein Subunits; D053701:Receptors, Interleukin-10; D016753:Interleukin-10; D007073:Immunoglobulin E