Ruegeria marina sp. nov., isolated from marine sediment.
A Gram-negative, neutrophilic and rod-shaped bacterium, strain ZH17(T), was isolated from a marine sediment of the East China Sea and subjected to a polyphasic taxonomic characterization. The isolate grew in the presence of 0-7.5 % (w/v) NaCl and at pH 6.5-9.0; optimum growth was observed with 0.5-3.0 % (w/v) NaCl and at pH 7.5. Chemotaxonomic analysis showed ubiquinone-10 as predominant respiratory quinone and C(18 : 1)ω7c, 11-methyl C(18 : 1)ω7c, C(16 : 0), C(12 : 0) 3-OH and C(16 : 0) 2-OH as major fatty acids. The genomic DNA G+C content was 63.5 mol%. Comparative 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Ruegeria. Strain ZH17(T) exhibited the closest phylogenetic affinity to the type strain of Ruegeria pomeroyi, with 97.2 % sequence similarity, and less than 97 % sequence similarity with respect to other described species of the genus Ruegeria. The DNA-DNA reassociation value between strain ZH17(T) and R. pomeroyi DSM 15171(T) was 50.7 %. On the basis of phenotypic and genotypic data, strain ZH17(T) represents a novel species of the genus Ruegeria, for which the name Ruegeria marina sp. nov. (type strain ZH17(T) =CGMCC 1.9108(T) =JCM 16262(T)) is proposed.
Huo
Ying-Yi
YY
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Xu
Xue-Wei
XW
Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.|Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou 310012, PR China.
Li
Xue
X
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Liu
Chen
C
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Cui
Heng-Lin
HL
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Wang
Chun-Sheng
CS
Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.|Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou 310012, PR China.
Wu
Min
M
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2