Association of the MCP-1 -2518 A/G polymorphism and no association of its receptor CCR2 -64 V/I polymorphism with lupus nephritis.
OBJECTIVE
To evaluate whether the A/G polymorphism at position -2518 in the regulatory region of the monocyte chemoattractant protein-1 (MCP-1) or the V/I polymorphism at position -64 of the receptor, CCR2, are associated with lupus nephritis (LN) or any clinical characteristics of the disease or with renal survival in a patient population.


METHODS
We selected 197 patients with lupus nephritis and 220 matched healthy controls for study. MCP-1 and CCR2 genotyping was performed by polymerase chain reaction. Clinical and laboratory data were compiled from patients' charts over followup that ranged from 6 months to 10 years.


RESULTS
The G/G genotype of MCP-1 was more common in LN patients (p = 0.019), while the A allele was associated with healthy controls (p = 0.007) as was the V allele of CCR2 (p = 0.046) compared to LN patients. Clinical index measures [SLE Disease Activity Index (SLEDAI)], immunological markers, renal histology, renal function at enrollment, and renal survival were not influenced by these polymorphisms. A less aggressive renal disease, measured by renal SLEDAI index, was associated with the V allele of the CCR2 gene polymorphism.


CONCLUSIONS
These findings support that MCP-1 -2518 G/G is associated with LN but there was no association of this genotype with renal function or renal survival. When studying CCR2 -64 V/I polymorphism we showed a positive association of the V allele with healthy controls but no association of the genotype with LN patients.
Nephrology Division, Department of Internal Medicine, São Paulo, SP, Brazil. patry@terra.com.br
Patricia
P
Malafronte
Jose Mauro
JM
Vieira
Alexandre Carlos
AC
Pereira
Jose Eduardo
JE
Krieger
Rui Toledo
RT
Barros
Viktoria
V
Woronik
D000328:Adult; D000483:Alleles; D000704:Analysis of Variance; D018932:Chemokine CCL2 / Q000235:genetics*; D003404:Creatinine / Q000097:blood; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007668:Kidney / Q000503:physiopathology; D007677:Kidney Function Tests; D008181:Lupus Nephritis / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D018579:Patient Selection; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D054390:Receptors, CCR2 / Q000235:genetics*; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric
D018932:Chemokine CCL2; D054390:Receptors, CCR2; D003404:Creatinine