Sensitive determination of tilmicosin, erythromycin ethylsuccinate and clindamycin by CE with electrochemiluminescence detection using azithromycin as internal standard and its applications.
A sensitive approach for the simultaneous determination of tilmicosin, erythromycin ethylsuccinate and clindamycin was developed by CE coupled with electrochemiluminescence detection with ionic liquid. The parameters for CE, electrochemiluminescence detection and the effect of ionic liquid were investigated systematically. The three analytes were well separated and detected within 8 min. The limits of detection (S/N=3) of tilmicosin, erythromycin ethylsuccinate and clindamycin are 3.4x10(-9), 2.3x10(-8) and 1.3x10(-8) mol/L, respectively. The precisions (RSD%) of the peak area and the migration time are from 0.8 to 1.5% and from 0.2 to 0.5% within a day and from 1.8 to 2.7% and from 0.6 to 0.8% in 3 days, respectively. The limits of quantitation (S/N=10) of tilmicosin, erythromycin ethylsuccinate and clindamycin are 3.2x10(-8), 2.9x10(-7) and 9.1x10(-8) mol/L in human urines and 5.5x10(-8), 3.2x10(-7) and 2.1x10(-7) mol/L in milk samples, respectively. The recoveries of three analytes at different concentration levels in urine, milk and drugs are between 90.0 and 104.7%. The proposed method was successfully applied to the determination of three analytes in human urine, milk and drugs.
Liu
Yan-Ming
YM
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, PR China. liuym9518@sina.com
Shi
Yan-Mei
YM
Liu
Zhuan-Li
ZL
Peng
Long-Fei
LF
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000652:urine; D017963:Azithromycin / Q000032:analysis*; D002021:Buffers; D002981:Clindamycin / Q000032:analysis* / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods*; D015643:Erythromycin Ethylsuccinate / Q000032:analysis* / Q000652:urine; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D049449:Luminescence; D008892:Milk / Q000737:chemistry; D012015:Reference Standards; D015645:Tylosin / Q000031:analogs & derivatives* / Q000032:analysis / Q000652:urine
D000900:Anti-Bacterial Agents; D002021:Buffers; D015643:Erythromycin Ethylsuccinate; D002981:Clindamycin; D017963:Azithromycin; C052319:tilmicosin; D015645:Tylosin