[Cloning of human scFv against soman transition state analogues (P6) from a large phage antibody library].
OBJECTIVE
To clone human anti-soman transition state analogues antibodies from a large single-chain phage antibody library.


METHODS
An organophosphorus hapten P6 [O1-methyl-O2-(1, 2, 2-trimethylpropyl)-2-hydroxy-5-nitrophenyl methylphosphonic acid] was synthesized. Its chemical conjugate with bovine serum albumin (BSA) was used as antigen (P6-BSA) to screen antibodies against soman. Panning of a large single-chain phage antibody library was conducted to select specific antibodies against soman. The antigen binding characteristics were analyzed by ELISA.


RESULTS
After 4 rounds of panning, 14 clones had specific binding ability to P6. DNA fingerprinting showed that diverse specific human scFvs against P6 was obtained from the library by biopanning.


CONCLUSIONS
Human anti-soman transition state analogues scFvs have been cloned from large phage antibody library.
Central Laboratory Navy General Hospital, Beijing, China.
Yu-xiao
YX
Wang
Pei-yuan
PY
Jia
Yu-xia
YX
Wang
Yuan-yuan
YY
Qiao
Ri-fang
RF
Yang
Li-jun
LJ
Zhou
D003001:Cloning, Molecular; D006801:Humans; D019151:Peptide Library; D057127:Single-Chain Antibodies / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D012999:Soman / Q000031:analogs & derivatives / Q000276:immunology*
D019151:Peptide Library; D057127:Single-Chain Antibodies; D012999:Soman