[Extract human DNA from maggot crop contents by phenol-chloroform method coupled with paramagnetic particle method].
OBJECTIVE
To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping.


METHODS
Human DNA was extracted from the maggot crop contents using phenol-chloroform method and purified by paramagnetic particle method. DNA was quantified by PCR with Quantifiler Human DNA Quantification Kit using 7500 real-time fluorescence quantitative PCR instrument. PCR products were genotyped by AmpFlSTR Identifiler PCR Amplification Kit using 3130XL-Avant genetic analyzer.


RESULTS
The template DNA yield by the method described were increased at least 2 times than the phenol-chloroform extraction method alone. All of the full 16 STR profiles could be obtained with the samples extracted by this method when the DNA yield reached (0.218 +/- 0.041) ng/microL.


CONCLUSIONS
Phenol-chloroform method coupled with paramagnetic particle method can effectively increase the sensitivity of STR analysis of human DNA recovered from maggot crop contents and is a valuable tool for forensic entomology.
Wang
Xiang
X
Department of Forensic Medicine, College of Preclinical Medicine, Central South University, Changsha 410013, China. wangx6281@163.com
Cai
Ji-Feng
JF
Zhong
Ming
M
Li
Jian-Bo
JB
Zhang
Jin-Guo
JG
Lan
Ling-Mei
LM
D000818:Animals; D002102:Cadaver; D002725:Chloroform / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D004175:Diptera / Q000235:genetics*; D004772:Entomology / Q000379:methods; D044707:Forensic Sciences / Q000379:methods; D005766:Gastrointestinal Contents; D006801:Humans; D007814:Larva / Q000235:genetics; D019800:Phenol / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences
D019800:Phenol; D002725:Chloroform; D004247:DNA