[Cloning of human scFv against soman transition state analogues (P6) from a large phage antibody library].
OBJECTIVE
To clone human anti-soman transition state analogues antibodies from a large single-chain phage antibody library.


METHODS
An organophosphorus hapten P6 [O1-methyl-O2-(1, 2, 2-trimethylpropyl)-2-hydroxy-5-nitrophenyl methylphosphonic acid] was synthesized. Its chemical conjugate with bovine serum albumin (BSA) was used as antigen (P6-BSA) to screen antibodies against soman. Panning of a large single-chain phage antibody library was conducted to select specific antibodies against soman. The antigen binding characteristics were analyzed by ELISA.


RESULTS
After 4 rounds of panning, 14 clones had specific binding ability to P6. DNA fingerprinting showed that diverse specific human scFvs against P6 was obtained from the library by biopanning.


CONCLUSIONS
Human anti-soman transition state analogues scFvs have been cloned from large phage antibody library.
Wang
Yu-xiao
YX
Central Laboratory Navy General Hospital, Beijing, China.
Jia
Pei-yuan
PY
Wang
Yu-xia
YX
Qiao
Yuan-yuan
YY
Yang
Ri-fang
RF
Zhou
Li-jun
LJ
D003001:Cloning, Molecular; D006801:Humans; D019151:Peptide Library; D057127:Single-Chain Antibodies / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D012999:Soman / Q000031:analogs & derivatives / Q000276:immunology*
D019151:Peptide Library; D057127:Single-Chain Antibodies; D012999:Soman