[Genomic analysis of DNA-protein interaction by chromatin immunoprecipitation].
Chromatin immunoprecipitation (ChIP) is an effective technique to analyze the interactions of DNA binding proteins with the genome in vivo. ChIP coupled with high density microarray (ChIP-chip) or high-throughput sequencing (ChIP-Seq) has generated large amount of data and expected to allow the development of a network describing the cellular transcriptional regulation. Here, we reviewed the ChIP, ChIP-chip, and ChIP-Seq techniques as well as their perspectives. Focus is given to data analysis of ChIP-Seq and the applications of ChIP-chip and ChIP-Seq.
Li
Min-Li
ML
The State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China. lml@seu.edu.cn
Wang
Wei
W
Lu
Zu-Hong
ZH
D000818:Animals; D047369:Chromatin Immunoprecipitation; D004247:DNA / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*
D011506:Proteins; D004247:DNA