Protection of red sea bream Pagrus major against red sea bream iridovirus infection by vaccination with a recombinant viral protein.
Megalocytivirus infections cause serious mass mortality in marine fish in East and Southeast Asian countries. In this study the immunogenicity of crude subunit vaccines against infection by the Megalocytivirus RSIV was investigated. Three capsid proteins, 18R, 351R and a major capsid protein, were selected for use as crude subunit vaccines. High homology among Megalocytivirus types was found in the initial sequence examined, the 351R region. Red sea bream (Pagrus major) juveniles were vaccinated by intraperitoneal injection of recombinant formalin-killed Escherichia coli cells expressing these three capsid proteins. After challenge infection with RSIV, fish vaccinated with the 351R-recombinant bacteria showed significantly greater survival than those vaccinated with control bacteria. The 351R protein was co-expressed with GAPDH from the bacterium Edwardsiella tarda in E. coli; this also protected against viral challenge. A remarkable accumulation of RSIV was observed in the blood of vaccinated fish, with less accumulation in the gills and spleen tissues. Thus, the 351R-GAPDH fusion protein is a potential vaccine against Megalocytivirus infection in red sea bream.
Laboratory of Cell Structure and Function, Division of Marine Bioresource Science, Graduate School of Kuroshio Science, Kochi University, Nankoku, Kochi, Japan.
Hajime
H
Shimmoto
Kenji
K
Kawai
Takuya
T
Ikawa
Syun-ichirou
S
Oshima
D000818:Animals; D036022:Capsid Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D004266:DNA Virus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D017994:Iridovirus / Q000276:immunology / Q000502:physiology*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D021541:Sea Bream / Q000276:immunology / Q000821:virology*; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
D036022:Capsid Proteins; D011994:Recombinant Proteins; D014765:Viral Vaccines