Local metabolism of glucocorticoids and its role in rat adjuvant arthritis.
11beta-Hydroxysteroid dehydrogenase 1 (11HSD1) regulates local glucocorticoid activity and plays an important role in various diseases. Here, we studied whether arthritis modulates 11HSD1, what is the role of pro-inflammatory cytokines in this process and whether altered local metabolism of glucocorticoids may contribute to the feedback regulation of inflammation. Adjuvant arthritis increased synovial 11HSD1 mRNA and 11-reductase activity but treatments with tumor necrosis factor alpha (TNF-alpha) and interleukin 1beta (IL-1beta) antagonists etanercept and anakinra reduced 11HSD1 upregulation. Treatment with carbenoxolone, an 11HSD inhibitor, increased expression of TNF-alpha, cyclooxygenase 2, and osteopontin mRNA without any changes in the plasma levels of corticosterone. Similar changes were observed when arthritic rats were treated with RU486, an antagonist of GR. This study suggests that arthritis upregulates synovial 11HSD1, this upregulation is controlled by TNF-alpha and IL-1beta and that the increased supply of local corticosterone might contribute to feedback regulation of inflammation.
Ergang
Peter
P
Institute of Physiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 14220 Prague 4-Krc, Czech Republic.
Leden
Pavel
P
Vagnerová
Karla
K
Klusonová
Petra
P
Miksík
Ivan
I
Jurcovicová
Jana
J
Kment
Milan
M
Pácha
Jirí
J
D042842:11-beta-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D001169:Arthritis, Experimental / Q000235:genetics / Q000378:metabolism*; D002229:Carbenoxolone / Q000494:pharmacology; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005938:Glucocorticoids / Q000378:metabolism*; D006727:Hormone Antagonists / Q000494:pharmacology; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008297:Male; D015735:Mifepristone / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D013583:Synovial Membrane / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D000897:Anti-Ulcer Agents; D016207:Cytokines; D005938:Glucocorticoids; D006727:Hormone Antagonists; D007527:Isoenzymes; D012333:RNA, Messenger; D015735:Mifepristone; D042842:11-beta-Hydroxysteroid Dehydrogenases; D002229:Carbenoxolone