Histologic and ultrastructural features of cryopreserved ovine ovarian tissue: deleterious effect of 1,2-propanediol applying different thawing protocols.
Ovarian cortical fragments were frozen in presence of 1.5 M PROH, thawed at different temperatures (37 degrees, 30 degrees, or 4 degrees C) and submitted to histologic and ultrastructural analysis. Thawing at 30 degrees C minimized stromal and follicular damage when compared with 4 degrees and 37 degrees C.
Oskam
Irma C
IC
Department of Obstetrics and Gynecology, Oslo University Hospital, Oslo, Norway. icaoska@smartcall.no
Asadi
Babak A
BA
Santos
Regiane R
RR
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005260:Female; D010053:Ovary / Q000187:drug effects* / Q000648:ultrastructure; D019946:Propylene Glycol / Q000494:pharmacology; D012756:Sheep; D013696:Temperature
D003451:Cryoprotective Agents; D019946:Propylene Glycol