In situ azide formation and "click" reaction of nile red with DNA as an alternative postsynthetic route.
Oligonucleotides that contain a 5-iodo-2'-deoxyuridine in the sequence can be applied for a postsynthetic "click"-type modification with ethynyl-modified nile red as a fluorescent label by in situ formation of the intermediate azide.
Institute for Organic Chemistry, University of Regensburg, 93040 Regensburg, Germany.
Christoph
C
Beyer
Hans-Achim
HA
Wagenknecht
D001386:Azides / Q000737:chemistry*; D001483:Base Sequence; D005456:Fluorescent Dyes / Q000737:chemistry*; D007065:Idoxuridine / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D010078:Oxazines / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D001386:Azides; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D010078:Oxazines; D007065:Idoxuridine; C044808:nile red