Ecophysiological responses of Abies fabri seedlings to drought stress and nitrogen supply.
Abies fabri (Mast.) Craib. (A. fabri) is an endemic and dominant species in typical subalpine dark coniferous forests distributed in mountainous regions of Western Sichuan, China. We investigated the ecophysiological responses of A. fabri seedlings to short-term experimental drought, nitrogen supply and their interaction. Drought stress was created by excluding natural precipitation with automatically controlled plastic roof that covered the seedlings. Nitrogen fertilization was applied weekly by spraying over seedlings ammonium nitrate solution (50 kg N ha(-1) year(-1)) during the growing season of 2009. The results showed that drought stress decreased leaf relative water content (RWC), whereas it caused marked increases in root mass ratio (RMR) and root/shoot mass ratio by 6.19 and 10.39%, respectively, as compared with the control. Drought stress increased malondialdehyde (MDA) content, electrolyte leakage, proline content, soluble sugars content and the activities of antioxidant enzymes, whereas nitrogen supply decreased MDA content, but enhanced activities of some antioxidant enzymes [especially peroxidase (POD)]. In the drought stressed plots, nitrogen supply increased RWC and decreased the content of MDA. The combination of drought stress and nitrogen supply also decreased the activities of antioxidant enzymes. These results indicated that the negative effects of drought stress on A. fabri seedlings might be alleviated by nitrogen supply.
Key Laboratory of Mountain Environmental Change and Regulation, Chengdu Institute of Mountain Hazards and Environment, The Alpine Ecosystem Observation and Experiment Station of the Gongga Mountain, Chinese Academy of Sciences & Ministry of Water Conservancy, Chengdu 610041, P.R. China.
Jianying
J
Guo
Yan
Y
Yang
Genxu
G
Wang
Liudong
L
Yang
Xiangyang
X
Sun
D028202:Abies / Q000201:enzymology / Q000502:physiology*; D000975:Antioxidants / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis; D002463:Cell Membrane Permeability; D055864:Droughts; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000032:analysis; D009584:Nitrogen / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D018515:Plant Leaves / Q000502:physiology; D018517:Plant Roots / Q000254:growth & development; D011392:Proline / Q000032:analysis; D036226:Seedlings / Q000201:enzymology / Q000502:physiology*; D026082:Singlet Oxygen / Q000378:metabolism; D014867:Water / Q000502:physiology
D000975:Antioxidants; D002241:Carbohydrates; D014867:Water; D026082:Singlet Oxygen; D008315:Malondialdehyde; D011392:Proline; D006861:Hydrogen Peroxide; D010544:Peroxidases; D009584:Nitrogen