Gel characterisation and in vivo evaluation of minocycline-loaded wound dressing with enhanced wound healing using polyvinyl alcohol and chitosan.
The purpose of this study was to develop a minocycline-loaded wound dressing with an enhanced healing effect. The cross-linked hydrogel films were prepared with polyvinyl alcohol (PVA) and chitosan using the freeze-thawing method. Their gel properties, in vitro protein adsorption, release, in vivo wound healing effect and histopathology were then evaluated. Chitosan decreased the gel fraction, maximum strength and thermal stability of PVA hydrogel, while it increased the swelling ability, water vapour transmission rate, elasticity and porosity of PVA hydrogel. Incorporation of minocycline (0.25%) did not affect the gel properties, and chitosan hardly affected drug release and protein adsorption. Furthermore, the minocycline-loaded wound dressing composed of 5% PVA, 0.75% chitosan and 0.25% drug was more swellable, flexible and elastic than PVA alone because of relatively weak cross-linking interaction of chitosan with PVA. In wound healing test, this minocycline-loaded PVA-chitosan hydrogel showed faster healing of the wound made in rat dorsum than the conventional product or the control (sterile gauze) due to antifungal activity of chitosan. In particular, from the histological examination, the healing effect of minocycline-loaded hydrogel was greater than that of the drug-loaded hydrogel, indicating the potential healing effect of minocycline. Thus, the minocycline-loaded wound dressing composed of 5% PVA, 0.75% chitosan and 0.25% drug is a potential wound dressing with excellent forming and enhanced wound healing.
Sung
Jung Hoon
JH
College of Pharmacy, Yeungnam University, Gyongsan, South Korea.
Hwang
Ma-Ro
MR
Kim
Jong Oh
JO
Lee
Jeong Hoon
JH
Kim
Yong Il
YI
Kim
Jeong Hoon
JH
Chang
Sun Woo
SW
Jin
Sung Giu
SG
Kim
Jung Ae
JA
Lyoo
Won Seok
WS
Han
Sung Soo
SS
Ku
Sae Kwang
SK
Yong
Chul Soon
CS
Choi
Han-Gon
HG
D000818:Animals; D047070:Bandages, Hydrocolloid; D048271:Chitosan / Q000737:chemistry*; D020100:Hydrogels; D008297:Male; D008855:Microscopy, Electron, Scanning; D008911:Minocycline / Q000008:administration & dosage* / Q000627:therapeutic use; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects / Q000293:injuries / Q000473:pathology; D012995:Solubility; D013499:Surface Properties; D013718:Tensile Strength; D014945:Wound Healing; D014947:Wounds and Injuries / Q000473:pathology / Q000628:therapy*
D020100:Hydrogels; D011142:Polyvinyl Alcohol; D048271:Chitosan; D008911:Minocycline