Mycobacterium tuberculosis complex differentiation by genomic deletion patterns with multiplex polymerase chain reaction and melting analysis.
Differentiation of Mycobacterium tuberculosis complex (MTC) species is important for patient management. We developed a genomic deletion assay based on multiplex polymerase chain reaction with melting temperature analysis that correctly identified 124 (96%) of 129 MTC isolates. This assay is a fast single-tube method to differentiate members of MTC.
Pounder
June I
JI
Institute for Clinical and Experimental Pathology, Associated Regional and University Pathologists, Inc., Salt Lake City, UT 84108, USA. afjip@uaa.alaska.edu
Anderson
Clint M
CM
Voelkerding
Karl V
KV
Salfinger
Max
M
Dormandy
Jillian
J
Somoskovi
Akos
A
Heifets
Leonid
L
Graham
Jenny Jo
JJ
Storts
Douglas R
DR
Petti
Cathy A
CA
D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017384:Sequence Deletion; D044366:Transition Temperature; D014376:Tuberculosis / Q000382:microbiology*
D004269:DNA, Bacterial