Differential recruitment of CD63 and Rab7-interacting-lysosomal-protein to phagosomes containing Mycobacterium tuberculosis in macrophages.
M.tb is an intracellular pathogen which survives within the phagosomes of host macrophages by inhibiting their fusion with lysosomes. Here, it has been demonstrated that a lysosomal glycoprotein, CD63, is recruited to the majority of M.tb phagosomes, while RILP shows limited localization. This is consistent with the author's findings that CD63, but not RILP, is recruited to the phagosomes in macrophages expressing the dominant negative form of Rab7. These results suggest that M.tb phagosomes selectively fuse with endosomes and lysosomes to escape killing activity while acquiring nutrients.
Seto
Shintaro
S
Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, 431-3192, Japan.
Matsumoto
Sohkichi
S
Tsujimura
Kunio
K
Koide
Yukio
Y
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D008247:Lysosomes / Q000378:metabolism / Q000382:microbiology; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000502:physiology*; D010588:Phagosomes / Q000378:metabolism* / Q000382:microbiology; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D060149:Tetraspanin 30; D014376:Tuberculosis / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000090802:rab7 GTP-Binding Proteins
D048868:Adaptor Proteins, Signal Transducing; D015703:Antigens, CD; D002352:Carrier Proteins; C554587:Cd63 protein, mouse; D010980:Platelet Membrane Glycoproteins; C498920:Rilp protein, mouse; D060149:Tetraspanin 30; D000090802:rab7 GTP-Binding Proteins; C000716465:rab7 GTP-binding proteins, mouse; D020691:rab GTP-Binding Proteins