Olfactory bulb proteins linked to olfactory memory in C57BL/6J mice.
Information on systematic analysis of olfactory memory-related proteins is poor. In this study, the odor discrimination task to investigate olfactory recognition memory of adult male C57BL/6J mice was used. Subsequently, olfactory bulbs (OBs) were taken, proteins extracted, and run on two-dimensional gel electrophoresis with in-gel-protein digestion, followed by mass spectrometry and quantification of differentially expressed proteins. Dual specificity mitogen-activated protein kinase kinase 1 (MEK1), dihydropyrimidinase-related protein 1 (DRP1), and fascin are related with Lemon odor memory. Microtubule-associated protein RP/EB family member 3 is related to Rose odor memory. Hypoxanthine-guanine phosphoribosyltransferase is related with both Lemon and Rose odors memory. MEK1 and DRP1 levels were increased, while microtubule-associated protein RP/EB family member 3, fascin and hypoxanthine-guanine phosphoribosyltransferase levels were decreased during olfactory memory. In summary, neurogenesis, signal transduction, cytoskeleton, and nucleotide metabolism are involved in olfactory memory formation and storage of C57BL/6J mice.
Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.
Lin
L
Li
Veronika
V
Mauric
Jun-Fang
JF
Zheng
Sung Ung
SU
Kang
Sudarshan
S
Patil
Harald
H
Höger
Gert
G
Lubec
D000818:Animals; D008297:Male; D051379:Mice / Q000502:physiology*; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009812:Odorants / Q000032:analysis; D009830:Olfactory Bulb / Q000737:chemistry / Q000378:metabolism*; D055696:Olfactory Perception; D011506:Proteins / Q000737:chemistry / Q000378:metabolism
D011506:Proteins