In vitro and in vivo evaluation of docetaxel loaded biodegradable polymersomes.
Formulation of docetaxel (DOC), a hydrophobic anticancer drug, was successfully achieved in poly(gamma-benzyl L-glutamate)-block-hyaluronan polymersomes using a simple and reproducible nanoprecipitation method. The prepared DOC loaded polymersomes (PolyDOC) was stable either in solution or in a lyophilized form, and showed controlled release behaviour over several days. PolyDOC showed high in vitro toxicity after 24 h in MCF-7 and U87 cells compared to free DOC. Biodistribution data demonstrated that (99m)Tc labelled PolyDOC t(1/2) and MRT significantly increased compared to a DOC solution (DS). In addition, PolyDOC uptake in Ehrlich Ascites Tumor (EAT) tumor bearing mice was larger at each time point compared to DS, making such a polymer vesicle formulation an efficient drug nanocarrier for improved DOC cancer therapy.
Université de Bordeaux, UMR5629, ENSCPB, 16 Avenue Pey Berland, 33607 Pessac Cedex, France.
Kamal K
KK
Upadhyay
Anant N
AN
Bhatt
Emilio
E
Castro
Anil K
AK
Mishra
Krishna
K
Chuttani
Bilikere S
BS
Dwarakanath
Christophe
C
Schatz
Jean-François
JF
Le Meins
Ambikanandan
A
Misra
Sébastien
S
Lecommandoux
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D002214:Capsules; D045744:Cell Line, Tumor; D003692:Delayed-Action Preparations / Q000737:chemistry*; D000077143:Docetaxel; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D011817:Rabbits; D043823:Taxoids / Q000008:administration & dosage* / Q000493:pharmacokinetics
D000970:Antineoplastic Agents; D002214:Capsules; D003692:Delayed-Action Preparations; D043823:Taxoids; C549034:poly(gamma-benzyl glutamate)-b-hyaluronan; D000077143:Docetaxel; D011099:Polyglutamic Acid; D006820:Hyaluronic Acid