Pentadecapeptide BPC 157 (PL 14736) improves ligament healing in the rat.
We improved medial collateral ligament (MCL) healing throughout 90 days after surgical transection. We introduced intraperitoneal, per-oral (in drinking water) and topical (thin cream layer) peptide therapy always given alone, without a carrier. Previously, as an effective peptide therapy, stable gastric pentadecapeptide BPC 157 (GEPPPGKPADDAGLV, an anti-ulcer peptide effective in inflammatory bowel disease therapy (PL 14736)) particularly improved healing of transected tendon and muscle and wound healing effect including the expression of the early growth response 1 (egr-1) gene. After MCL transection BPC 157 was effective in rats when given once daily intraperitoneally (10 microg or 10 ng/kg) or locally as a thin layer (1.0 microg dissolved in distilled water/g commercial neutral cream) at the site of injury, first application 30 min after surgery and the final application 24 h before sacrifice. Likewise, BPC 157 was effective given per-orally (0.16 microg/ml in the drinking water (12 ml/day/rat)) until sacrifice. Commonly, BPC 157 microg-ng-rats exhibited consistent functional, biomechanical, macroscopic and histological healing improvements. Thus, we suggest BPC 157 improved healing of acute ligament injuries in further ligament therapy.
Cerovecki
Tomislav
T
University Hospital of Traumatology, Zagreb, Croatia.
Bojanic
Ivan
I
Brcic
Luka
L
Radic
Bozo
B
Vukoja
Ivan
I
Seiwerth
Sven
S
Sikiric
Predrag
P
D000284:Administration, Oral; D000818:Animals; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D003286:Contracture / Q000517:prevention & control; D004195:Disease Models, Animal; D008297:Male; D017888:Medial Collateral Ligament, Knee / Q000187:drug effects* / Q000293:injuries* / Q000502:physiology; D009043:Motor Activity / Q000187:drug effects; D009504:Neutrophils / Q000502:physiology; D010446:Peptide Fragments / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
D024042:Collagen Type I; D024061:Collagen Type III; D010446:Peptide Fragments; D011506:Proteins; C083833:BPC 157