Evaluation of photo-induced cellular damage using photoautotrophic cultures of puk-bung hairy roots as a sensing tool.
In photoautotrophic cultures of pak-bung hairy roots, strong light irradiation (22 W/m(2)) increased the content of reactive oxygen species (ROS) in the cells, resulting in chlorophyll (Chl) degradation and DNA injury. The Chl degradation rate, R(D), can be used as a parameter to measure the cellular damage caused by photo-induced stress. The presence of the antioxidants ascorbic acid, chlorogenic acid or catechin reduced the R(D), while lowering the content of ROS and moderating the DNA injury.
Nishioka
Motomu
M
Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Ojima
Yoshihiro
Y
Tsushima
Yuki
Y
Matsumoto
Masahiro
M
Taya
Masahito
M
D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D001709:Biotechnology; D002392:Catechin / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000494:pharmacology; D002734:Chlorophyll / Q000378:metabolism; D004249:DNA Damage; D018744:DNA, Plant / Q000378:metabolism / Q000528:radiation effects; D027741:Ipomoea / Q000378:metabolism* / Q000528:radiation effects*; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D018462:Photobiology; D018517:Plant Roots / Q000378:metabolism / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D046509:Tissue Culture Techniques
D000975:Antioxidants; D018744:DNA, Plant; D017382:Reactive Oxygen Species; D002734:Chlorophyll; D002726:Chlorogenic Acid; D002392:Catechin; D001205:Ascorbic Acid