Microtubule depolymerization at high pressure.
We performed in vitro assays to visualize the effects of pressure on the filamentous structure of microtubules. Taxol-stabilized microtubules were tethered to kinesin motors on the observation window of a high-pressure chamber. When pressure was applied to the sample solution, all of the microtubules started to shorten from both ends. The length changes were constant over time, irrespective of the microtubule polarity. The shortening rate of microtubules increased exponentially with pressure, and the activation volume was -100 mL/mol, consistent with in vivo studies. These results show that application of pressure works directly to weaken the intermolecular interactions between tubulin molecules.
Nishiyama
Masayoshi
M
Department of Chemistry, Kyoto University, Japan. m-nishi@kuchem.kyoto-u.ac.jp
Shimoda
Yoshiki
Y
Hasumi
Manabu
M
Kimura
Yoshifumi
Y
Terazima
Masahide
M
D001709:Biotechnology / Q000295:instrumentation; D003599:Cytoskeleton / Q000737:chemistry; D006874:Hydrostatic Pressure; D066298:In Vitro Techniques; D016547:Kinesins / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D008870:Microtubules / Q000737:chemistry* / Q000648:ultrastructure*; D020409:Molecular Motor Proteins / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D014404:Tubulin / Q000737:chemistry; D014867:Water / Q000737:chemistry
D020409:Molecular Motor Proteins; D011108:Polymers; D014404:Tubulin; D014867:Water; D016547:Kinesins