In-depth exploration of Hevea brasiliensis latex proteome and "hidden allergens" via combinatorial peptide ligand libraries.
The proteome of Hevea brasiliensis latex has been explored in depth via combinatorial peptide ligand libraries. A total of 300 unique gene products have been identified in this latex, whose proteome has been largely unknown up to the present. In search for unknown allergens, control latex and eluates from the ligand libraries have been fractionated by two-dimensional mapping, blotted and confronted with sera of 18 patients. In addition to the already known and named Hevea major allergens, we have unambiguously detected several others like, for instance: heat shock protein (81 kDa), proteasome subunit (30 kDa), protease inhibitor (8 kDa), hevamine A (43 kDa) and glyceraldehyde-3-phosphate dehydrogenase (37 kDa). Gene Ontology analysis of analyzed fractions has shown that major functions are substantially unchanged after sample treatment, while novel biological functions appeared that were undetectable in the crude sample.
D'Amato
Alfonsina
A
Division of Genetics and Cell Biology, San Raffaele Scientific Institute, 20132 Milano, Italy.
Bachi
Angela
A
Fasoli
Elisa
E
Boschetti
Egisto
E
Peltre
Gabriel
G
Sénéchal
Hélène
H
Sutra
Jean Pierre
JP
Citterio
Attilio
A
Righetti
Pier Giorgio
PG
D052179:Antigens, Plant / Q000737:chemistry* / Q000276:immunology*; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D028482:Hevea / Q000276:immunology*; D006801:Humans; D007840:Latex / Q000276:immunology*; D020315:Latex Hypersensitivity / Q000276:immunology; D019151:Peptide Library; D020543:Proteome / Q000737:chemistry* / Q000276:immunology*
D052179:Antigens, Plant; D007840:Latex; D019151:Peptide Library; D020543:Proteome