Secreted surfactant protein A from fetal membranes induces stress fibers in cultured human myometrial cells.
In the present study, we investigated the ability of human fetal membranes (amnion and choriodecidua) to regulate human maternal uterine cell functions through the secretion of surfactant protein (SP)-A and SP-D at the end of pregnancy. We detected the expression of both SP-A (SP-A1 and SP-A2) and SP-D by quantitative reverse transcription polymerase chain reaction. Immunohistochemistry revealed that human fetal membranes expressed both SP-A and SP-D. By Western blot analysis, we demonstrated that SP-A protein expression was predominant in choriodecidua, whereas the amnion predominantly expressed SP-D. Only the secretion of SP-A was evidenced in the culture supernatants of amnion and choriodecidua explants by immunodot blot and confirmed by Western blot. Exogenous human purified SP-A induced stress fiber formation in cultured human myometrial cells via a pathway involving Rho-kinase. Conditioned medium from choriodecidua and amnion explants mimicked the SP-A effect. Treatment of myometrial cells with SP-A-depleted conditioned medium from choriodecidua or amnion explants failed to change the actin dynamic. These data indicate that SP-A released by human fetal membranes is able to exert a paracrine regulation of F-actin filament organization in myometrial cells.
Breuiller-Fouché
Michelle
M
Institut National de la Santé et de la Recherche Médicale (INSERM), U767, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Paris V, Paris, France. michelle.breuiller-fouche@inserm.fr
Dubois
Olivier
O
Sediki
Mourad
M
Garcia-Verdugo
Ignacio
I
Palaniyar
Nades
N
Tanfin
Zahra
Z
Chissey
Audrey
A
Cabrol
Dominique
D
Charpigny
Gilles
G
Mehats
Céline
C
D000199:Actins / Q000378:metabolism; D015153:Blotting, Western; D002478:Cells, Cultured; D005321:Extraembryonic Membranes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence; D009215:Myometrium / Q000187:drug effects* / Q000502:physiology; D011247:Pregnancy; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D037663:Pulmonary Surfactant-Associated Protein D / Q000235:genetics / Q000494:pharmacology*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D022502:Stress Fibers / Q000187:drug effects* / Q000502:physiology
D000199:Actins; D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D012313:RNA