Lentiviral vector-mediated transgenesis in human embryonic stem cells.
Human Embryonic stem cells (hESCs) offer an invaluable tool for revealing human biology and a potential source of functional cells/tissues for regenerative medicine. The utility of hESCs will likely be significantly enhanced and broadened by our ability to build versatile genetically modified hESC lines. Here, we describe an efficient lentiviral vector mediated method to establish stable transgenic hESCs.
Du
Zhong-Wei
ZW
Departments of Anatomy and Neurology, School of Medicine and Public Health, WiCell Institute, University of Wisconsin, Madison, WI, USA.
Zhang
Su-Chun
SC
D002460:Cell Line; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D011401:Promoter Regions, Genetic; D044968:Regenerative Medicine; D014162:Transfection; D019076:Transgenes