Effect of PBNA on the NO content and NOS activity in ischemia/reperfusion injury in the rat retina.
OBJECTIVE
To investigated the effect of Polygonum Bistorta L. n-butyl Alcohol (PBNA) extract on the NO content and NOS activity in ischemia/reperfusion (I/R) injury in the rat retina.


METHODS
The model of retinal I/R injury in SD rats was made by reperfusion for 1 h after occlusion of common carotid artery (CCA) for 1 h. The rats were randomly divided into four groups: control group, retinal I/R injury group, low-dosage PBNA treated group and high-dosage treated PBNA group. The control group was injected with 1 ml/kg NS through sublingual vein after CCA was dissociated. Other groups were treated with normal saline or PBNA before occlusion of CCA. After occlusion of CCA for 1 h following reperfusion for 1 h, blood was collected and serum was separated to determine the contents of NO, the activity of T-NOS, iNOS and eNOS.


RESULTS
(1) The contents of NO in I/R group showed lower values than in control group (P<0.001) and low-dosage PBNA treated group (P<0.05). (2) The activities of T-NOS in both low-dosage PBNA group and high-dosage group increased, compared with I/R group (P<0.01). (3) The activity of serum iNOS in I/R group increased compared with control group (P<0.05) and low-dosage PBNA treated group evidently (P<0.05). (4) The activity of serum eNOS in I/R group decreased compared with control group (P<0.05), both low-dosage (P<0.05) and high-dosage PBNA (P<0.01) treated group markedly.


CONCLUSIONS
The date suggest that PBNA have a therapeutic effect on retinal ischemia/reperfusion injury by increasing the activities of T-NOS and eNOS, decreasing the activity of iNOS, elevating the content of NO, enhancing the Anti-oxidation and expanding the blood vessel.
Preclinical Medical College, Gannan Medical University, Ganzhou, Jiangxi Province, China.
Liangdong
L
Li
Zhihua
Z
Huang
Hai
H
Xiao
Xigui
X
Chen
Jing
J
Zeng
D000818:Animals; D008297:Male; D009569:Nitric Oxide / Q000097:blood*; D019001:Nitric Oxide Synthase / Q000097:blood*; D052247:Nitric Oxide Synthase Type II / Q000097:blood; D052250:Nitric Oxide Synthase Type III / Q000097:blood; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D028502:Polygonum / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood* / Q000188:drug therapy* / Q000201:enzymology; D012160:Retina / Q000187:drug effects / Q000201:enzymology / Q000473:pathology
D010936:Plant Extracts; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III