Single round of antigen receptor signaling programs naive B cells to receive T cell help.
To simulate transient B cell activation that is the likely initiator of T-dependent responses, we examined the molecular and functional consequences of a single round of immunoglobulin M (IgM) signaling. This form of activation triggered early cytosolic signaling and the transcription factor NF-kappaB activation indistinguishably from conventional continuous IgM crosslinking but did not induce G1 progression. However, single round IgM signaling changed the expression of chemokine and chemokine receptor genes implicated in initiating T-dependent responses, as well as accentuated responsiveness to CD40 signaling. Several features of single-round IgM signaling in vitro were recapitulated in B cells after short-term exposure to antigen in vivo. We propose that transient BCR signals prime B cells to receive T cell help by increasing the probability of B-T encounter and creating a cellular environment that is hyper-responsive to CD40 signaling.
Gene Regulation Section, Laboratory of Cellular and Molecular Biology, National Institute on Aging, Baltimore, MD 21224, USA.
Bazarragchaa
B
Damdinsuren
Yongqing
Y
Zhang
Ashraf
A
Khalil
William H
WH
Wood
Kevin G
KG
Becker
Mark J
MJ
Shlomchik
Ranjan
R
Sen
D056704:Adaptive Immunity; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D019013:CD40 Antigens / Q000276:immunology / Q000378:metabolism; D016193:G1 Phase; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D011947:Receptors, Antigen, B-Cell / Q000276:immunology* / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000276:immunology* / Q000378:metabolism; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D019013:CD40 Antigens; D011947:Receptors, Antigen, B-Cell; D011948:Receptors, Antigen, T-Cell