Arginine methylation of the B cell antigen receptor promotes differentiation.
Signals processed through the B cell antigen receptor (BCR) control both the proliferation and differentiation of B lymphocytes. How these different signaling modes are established at the BCR is poorly understood. We show that a conserved arginine in the tail sequence of the Igalpha subunit of the BCR is methylated by the protein arginine methyltransferase 1. This modification negatively regulates the calcium and PI-3 kinase pathways of the BCR while promoting signals leading to B cell differentiation. Thus, Igalpha arginine methylation can play an important role in specifying the outcome of BCR signaling.
Center for Biological Signaling Studies, Faculty of Biology, Albert-Ludwigs-University of Freiburg, 79104 Freiburg, Germany.
Simona
S
Infantino
Beate
B
Benz
Tanja
T
Waldmann
Manfred
M
Jung
Robert
R
Schneider
Michael
M
Reth
D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000235:genetics / Q000378:metabolism*; D016175:B-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism / Q000637:transplantation; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism* / Q000637:transplantation; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D051925:CD79 Antigens / Q000235:genetics / Q000378:metabolism; D020013:Calcium Signaling / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D006801:Humans; D015851:Interleukin-7 / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008213:Lymphocyte Activation / Q000502:physiology; D008745:Methylation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009592:Nitrohydroxyiodophenylacetate / Q000276:immunology; D010766:Phosphorylation / Q000276:immunology; D054448:Precursor Cells, B-Lymphoid / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011485:Protein Binding / Q000276:immunology; D011484:Protein-Arginine N-Methyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D011947:Receptors, Antigen, B-Cell / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000166:cytology / Q000276:immunology; D000072377:Syk Kinase; D014162:Transfection
D051925:CD79 Antigens; D015851:Interleukin-7; D047908:Intracellular Signaling Peptides and Proteins; D034741:RNA, Small Interfering; D011947:Receptors, Antigen, B-Cell; D009592:Nitrohydroxyiodophenylacetate; D001120:Arginine; C546338:Prmt1 protein, mouse; D011484:Protein-Arginine N-Methyltransferases; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase; C000606897:Syk protein, mouse; D051057:Proto-Oncogene Proteins c-akt