Identification and characterization of two phospholipid hydroperoxide glutathione peroxidase genes from Apis cerana cerana.
Phospholipid hydroperoxide glutathione peroxidase (PHGPX) plays a crucial role in maintaining the integrity of membrane by reducing hydroperoxides of phospholipids. Here, we report the identification and characterization of two genes, designated AccGtpx-1 and AccGtpx-2, encoding PHGPX proteins from the Chinese honeybees, Apis cerana cerana. Alignment analysis showed that AccGtpx-1 and AccGtpx-2 shared high similarity with other known PHGPXs, which show similar structure to thioredoxin. These single copy genes showed complex exon-intron structures. The mRNA of AccGtpx-1 was detected in larvae, pupae and adults and that AccGtpx-2 was only found in adult worker bees. Furthermore, the expression of AccGtpx-1 could be induced by H(2)O(2), ultraviolet (UV) light, heat shock (37 degrees C), HgCl(2), imidacloprid, cyhalothrin, pyriproxyfen and methomyl. In contrast, AccGtpx-2 expression could only be induced by UV. These results indicated for the first time that the AccGtpx-1 and AccGtpx-2 genes encoding A. cerana cerana PHGPXs are regulated differently in response to environmental stressors.
Wang
Mian
M
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, PR China.
Kang
Mingjiang
M
Guo
Xingqi
X
Xu
Baohua
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000201:enzymology* / Q000235:genetics*; D005979:Glutathione Peroxidase / Q000235:genetics*; D008969:Molecular Sequence Data; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods
D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase