[Expression of FAP and alpha-SMA during the incised wound healing in mice skin].
OBJECTIVE To investigate the time-dependent expression of fibroblast activation protein (FAP) and alpha-smooth muscle actin(alpha-SMA) during the incised wound healing of the skin in mice.


METHODS
The expression of FAP and alpha-SMA in incised wound of mice skin was detected by immunohistochemistry and Western blot.


RESULTS
By immunohistochemistry, the expression of FAP and alpha-SMA in the normal skin and the skin 1 h after injury maintained at a very low level, but the positive cells expressing FAP and alpha-SMA started to elevate 6 h after injury and reached its peak on 5 d for FAP and on 3 d for alpha-SMA, then gradually decreased to the normal level on 14 d. The expression of FAP and alpha-SMA was observed throughout the wound healing stages 1 d after injuries by Western blot as well with a peak expression occurring on 5 d for FAP and on 3 d for alpha-SMA after injury.


CONCLUSIONS
FAP may be a potentially useful marker for wound age determination and alpha-SMA may be used as an effective indicator for the mid- and late stage incised wound of mice skin. The combination use of FAP and alpha-SMA may be potentially effective indicators for wound age determination.
Department of Forensic Pathology, Harbin Medical University, Harbin 150081, China. gaoyangmail@126.com
Yang
Y
Gao
Xue
X
Peng
Zhan-Fen
ZF
Jin
Zhi-Jun
ZJ
Fu
D000199:Actins / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D010450:Endopeptidases; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D049429:Forensic Pathology; D018093:Gelatinases / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D011897:Random Allocation; D012697:Serine Endopeptidases / Q000378:metabolism*; D012867:Skin / Q000293:injuries* / Q000378:metabolism*; D013997:Time Factors; D014945:Wound Healing; D014947:Wounds and Injuries / Q000378:metabolism / Q000503:physiopathology; D000099167:Fibroblast Activation Protein Alpha
D000199:Actins; D010450:Endopeptidases; D018093:Gelatinases; D008565:Membrane Proteins; D012697:Serine Endopeptidases; D000099167:Fibroblast Activation Protein Alpha; C558396:Acta2 protein, mouse