Dendritic chain reaction: responsive release of hydrogen peroxide upon generation and enzymatic oxidation of methanol.
Signal amplification dramatically increases the sensitivity of diagnostic methods. Recently, we introduced a new technique for signal amplification that uses a distinctive dendritic chain reaction (DCR) to generate exponential evolution of a diagnostic signal. In this report, we demonstrate how the modular design of our DCR probe can be used to improve the detection sensitivity. We synthesized a new probe based on a methyl carbonate linkage, which has superior stability in aqueous media. Triggered release of methanol, which was oxidized by alcohol oxidase present in the solution, produced hydrogen peroxide that used as a reagent in the DCR amplification technique. The new probe exhibited higher sensitivity in detection of hydrogen peroxide than our previously reported probe.
Avital-Shmilovici
Michal
M
Department of Organic Chemistry, Tel-Aviv University, Tel Aviv 69978, Israel.
Shabat
Doron
D
D000429:Alcohol Oxidoreductases / Q000032:analysis / Q000378:metabolism*; D019937:Diagnostic Techniques and Procedures; D006861:Hydrogen Peroxide / Q000032:analysis*; D000432:Methanol / Q000378:metabolism; D015335:Molecular Probes; D010084:Oxidation-Reduction
D015335:Molecular Probes; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D000432:Methanol