[The effect of gastrodine on hepatitic cell line L02 injury induced by ethanol].
OBJECTIVE
To explore the the effect of gastrodine on hepatitic cell line L02 injury induced by ethanol.


METHODS
The growing L02 cells were treated with different concentrations of alcohol. For screening the proper concentration of alcohol, the proliferation of the cells were measured by MTT colorimetry. The level of reactive oxygen species (ROS) and change of mitochondrial membrane potential in cells were tested by flow cytometry. Reversed-phase high-performance liquid chromatography (RP-HPLC) was applied to assay ATP content in cells.


RESULTS
The cells were injured when treated with 25 mL/L alcohol for 36 h. Compared with the control group , the ROS level in cells markedly increased while mitochondrial membrane potential and ATP content in cells significantly decreased (P<0.01). Gastrodine obviously inhibited cell damage, reduced ROS level, and increased mitochondrial membrane potential and ATP content in cells.


CONCLUSIONS
Above results suggest that gastrodine may suppress cellular injury and lipid peroxidation induced by ethanol, improve mitochondrial function and raise ATP level, which play an important role in protecting hepatocytes.
Department of Physiology, Medical College of Jinan University, Guangzhou, China. tbzq@jnu.edu.cn
Zhi-quan
ZQ
Bai
Chao-feng
CF
Hu
Li-ping
LP
Sun
D001592:Benzyl Alcohols / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
D001592:Benzyl Alcohols; D005960:Glucosides; D017382:Reactive Oxygen Species; D000431:Ethanol; C045345:gastrodin