Molecular and clinical features of chronic lymphocytic leukemia with stereotyped B-cell receptors in a Ukrainian cohort.
A fraction of chronic lymphocytic leukemia (CLL) carries highly homologous B-cell receptors, characterized by non-random combinations of immunoglobulin heavy-chain variable (IGHV) genes and heavy-chain complementarity-determining region-3 (HCDR3), often associated with a restricted selection of IG(K/L)V light chains. We analyzed the features of CLL expressing homologous HCDR3 in a cohort of 264 Ukrainian patients by merging them with a recently published reference series of 1426 cases. This approach allowed us to identify 96/264 (36%) cases as expressing homologous HCDR3, subdivided into 47 subsets. Among these, 27 apparently novel subsets were identified, although most of them were composed of two sequences per subset ('potential subsets'). CLL cases belonging to several stereotyped subsets showed HCDR3 homologies with various autoreactive clones. Our analysis identified molecular and clinical features of a Ukrainian cohort of patients with CLL.
Bilous
Nadiia
N
Research Center for Radiation Medicine, Academy of Medical Sciences, Kiev, 04053, Ukraine. nbilous@yahoo.com
Bomben
Riccardo
R
Dal Bo
Michele
M
Capello
Daniela
D
Forconi
Francesco
F
Laurenti
Luca
L
Bertoni
Francesco
F
Efremov
Dimitar G
DG
Marasca
Roberto
R
Del Poeta
Giovanni
G
Martina
Zoya
Z
Kryachouk
Iryna
I
Dyagil
Iryna
I
Gaidano
Gianluca
G
Chumak
Anatoliy
A
Gattei
Valter
V
Abramenko
Iryna
I
D015331:Cohort Studies; D022801:Complementarity Determining Regions / Q000235:genetics; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics; D050438:Genes, Immunoglobulin Heavy Chain / Q000235:genetics; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology*; D011947:Receptors, Antigen, B-Cell / Q000235:genetics*; D027041:Somatic Hypermutation, Immunoglobulin / Q000235:genetics; D015996:Survival Rate; D016896:Treatment Outcome
D022801:Complementarity Determining Regions; D007135:Immunoglobulin Variable Region; D011947:Receptors, Antigen, B-Cell