Recovery of tobacco BY-2 cells after high hydrostatic pressure treatment.
The recovery of Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) cells in Linsmaire and Skoog medium after treatment at high hydrostatic pressure was investigated using an Evans Blue staining method to discriminate live from dead cells. The survival of BY-2 cells just after the high-pressure treatment at 5 degrees C and 25 degrees C decreased abruptly at pressures higher than 50 MPa and 100 MPa, respectively. Furthermore, almost all of the BY-2 cells treated at 5 degrees C and 25 degrees C recovered pressures below 25 MPa and 75 MPa, respectively. However, no BY-2 cells recovered at pressures above 100 MPa at either temperature.
Department of Material Science, Wakayama National College of Technology, Noshima, Gobo, Japan.
Masataka
M
Kusube
Takumi
T
Nishino
Yuki
Y
Nishikawa
Masaki
M
Goto
Hitoshi
H
Matsuki
Hitoshi
H
Iwahashi
D001709:Biotechnology; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D006874:Hydrostatic Pressure; D011309:Preservation, Biological / Q000379:methods*; D013696:Temperature; D014026:Nicotiana / Q000166:cytology*