Enhancement of biofilm formation by subinhibitory concentrations of macrolides in icaADBC-positive and -negative clinical isolates of Staphylococcus epidermidis.
Biofilm formation in Staphylococcus epidermidis is mediated by icaADBC-dependent and -independent pathways. Subinhibitory concentrations of erythromycin, azithromycin, and clarithromycin enhanced, in a dose-dependent manner, the level of biofilm formation by 20% (21/105 isolates) by macrolide-resistant ica-positive and -negative isolates tested in vitro. The presence of ica, however, apparently produced an enhanced effect on biofilm formation. The levels of expression of the biofilm-related genes icaA, atlE, fruA, pyrR, sarA, and sigB were increased in response to erythromycin. The results likely underscore the potential clinical relevance of macrolide-induced biofilm growth.
Wang
Qian
Q
Department of Pharmacy, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
Sun
Feng-Jun
FJ
Liu
Yao
Y
Xiong
Li-Rong
LR
Xie
Lin-Li
LL
Xia
Pei-Yuan
PY
D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001483:Base Sequence; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D017931:DNA Primers / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004917:Erythromycin / Q000008:administration & dosage; D015870:Gene Expression / Q000187:drug effects; D005798:Genes, Bacterial; D006801:Humans; D066298:In Vitro Techniques; D018942:Macrolides / Q000008:administration & dosage*; D008855:Microscopy, Electron, Scanning; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology*; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D018942:Macrolides; D004917:Erythromycin