Folding anomalies of neuroligin3 caused by a mutation in the alpha/beta-hydrolase fold domain.
Proteins of the alpha/beta-hydrolase fold family share a common structural fold, but perform a diverse set of functions. We have been studying natural mutations occurring in association with congenital disorders in the alpha/beta-hydrolase fold domain of neuroligin (NLGN), butyrylcholinesterase (BChE), acetylcholinesterase (AChE). Starting from the autism-related R451C mutation in the alpha/beta-hydrolase fold domain of NLGN3, we had previously shown that the Arg to Cys substitution is responsible for endoplasmic reticulum (ER) retention of the mutant protein and that a similar trafficking defect is observed when the mutation is inserted at the homologous positions in AChE and BChE. Herein we show further characterization of the R451C mutation in NLGN3 when expressed in HEK-293, and by protease digestion sensitivity, we reveal that the phenotype results from protein misfolding. However, the presence of an extra Cys does not interfere with the formation of disulfide bonds as shown by reaction with PEG-maleimide and estimation of the molecular mass changes. These findings highlight the role of proper protein folding in protein processing and localization.
Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093, United States. antonella.dejaco@uniroma1.it
Antonella
A
De Jaco
Noga
N
Dubi
Davide
D
Comoletti
Palmer
P
Taylor
D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D004220:Disulfides / Q000737:chemistry; D006801:Humans; D006867:Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D017510:Protein Folding; D017434:Protein Structure, Tertiary
D015816:Cell Adhesion Molecules, Neuronal; D004220:Disulfides; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C098004:neuroligin 3; D006867:Hydrolases