Attenuation of Vibrio fischeri quorum sensing using rationally designed polymers.
A first attempt to attenuate the quorum sensing (QS) of a marine heterotroph microorganism, Vibrio fischeri , using signal molecule-sequestering polymers (SSPs) is presented. A set of rationally designed polymers with affinity toward a signal molecule of V. fischeri , N-(beta-ketocaproyl)-l-homoserine lactone (3-oxo-C6-AHL) was produced. It is reported that computationally designed polymers could sequester a signal molecule of V. fischeri and prevent QS-controlled phenotypes (in this case, bioluminescence) from being up-regulated. It was proven that the attenuation of bioluminescence of V. fischeri was due to sequestration of the signal molecule by specific polymers and not due to the toxicity of polymer or nonspecific depletion of nutrients. The ability to disrupt the bacterial communication using easy to synthesize and chemically inert polymers could provide a new concept for the development of pharmaceuticals and susceptible device coatings such as catheters.
Piletska
Elena V
EV
Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, United Kingdom. e.piletska@cranfield.ac.uk
Stavroulakis
Georgios
G
Karim
Kal
K
Whitcombe
Michael J
MJ
Chianella
Iva
I
Sharma
Anant
A
Eboigbodin
Kevin E
KE
Robinson
Gary K
GK
Piletsky
Sergey A
SA
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D048908:Aliivibrio fischeri / Q000502:physiology*; D019295:Computational Biology; D015195:Drug Design; D008163:Luminescent Measurements; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D053038:Quorum Sensing / Q000187:drug effects*; D014735:Vibrio Infections / Q000188:drug therapy* / Q000378:metabolism
D011108:Polymers; C030654:N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone; D015107:4-Butyrolactone