Biochemical methods used to study the gene expression and protein complexes in the filamentous fungus Neurospora crassa.
Biochemical approaches are powerful tools for investigating mechanisms of biological processes. Here, we describe several biochemical approaches that have been successfully in our laboratory to study the filamentous fungus Neurospora crassa. These approaches include protein extraction and western blot analysis, protein purification using epitope-tagged fusion protein, protein immunoprecipitation (IP) and Chromatin Immunoprecipitation (ChIP) assays. These methods can also be modified for use in other filamentous fungi.
Department of Physiology, The University of Texas Southwestern Medical Center, Dallas, TX, USA.
Jinhu
J
Guo
Guocun
G
Huang
Joonseok
J
Cha
Yi
Y
Liu
D015153:Blotting, Western / Q000379:methods*; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D005656:Fungal Proteins / Q000378:metabolism*; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D047468:Immunoprecipitation / Q000379:methods*; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins