The role of the cysteine residue in the chaperone and anti-apoptotic functions of human Hsp27.
The small heat shock protein Hsp27 is a molecular chaperone and an anti-apoptotic protein. Human Hsp27 has one cysteine residue at position 137. We investigated the role of this cysteine residue in the chaperone and anti-apoptotic functions of Hsp27 by mutating the cysteine residue to an alanine (Hsp27(C137A)) and comparing it to wild-type protein (Hsp27(WT)). Both proteins were multi-subunit oligomers, but subunits of Hsp27(WT) were disulfide-linked unlike those of Hsp27(C137A), which were monomeric. Hsp27(C137A) was indistinguishable from Hsp27(WT) with regard to its secondary structure, surface hydrophobicity, oligomeric size and chaperone function. S-thiolation and reductive methylation of the cysteine residue had no apparent effect on the chaperone function of Hsp27(WT). The anti-apoptotic function of Hsp27(C137A) and Hsp27(WT) was studied by overexpressing them in CHO cells. No difference in the caspase-3 or -9 activity was observed in staurosporine-treated cells. The rate of apoptosis between Hsp27(C137A) and Hsp27(WT) overexpressing cells was similar whether the cells were treated with staurosporine or etoposide. However, the mutant protein was less protective relative to the wild-type protein in preventing caspase-3 and caspase-9 activation and apoptosis induced by 1 mM H(2)O(2) in CHO and HeLa cells. These data demonstrate that in human Hsp27, disulfide formation by the lone cysteine does not affect its chaperone function and anti-apoptotic function against chemical toxicants. However, oxidation of the lone cysteine in Hsp27 might at least partially affect the anti-apoptotic function against oxidative stress.
Department of Ophthalmology & Visual sciences, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Nagarekha
N
Pasupuleti
Mahesha
M
Gangadhariah
Smitha
S
Padmanabha
Puttur
P
Santhoshkumar
Ram H
RH
Nagaraj
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D016466:CHO Cells; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002942:Circular Dichroism; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D003545:Cysteine / Q000502:physiology*; D017931:DNA Primers; D055551:HSP27 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D017931:DNA Primers; D055551:HSP27 Heat-Shock Proteins; D053148:Caspase 3; D053453:Caspase 9; D003545:Cysteine