Site-specific labeling of nucleotides for making RNA for high resolution NMR studies using an E. coli strain disabled in the oxidative pentose phosphate pathway.
Dayie
T Kwaku
TK
Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, 1115 Biomolecular Sciences Bldg (#296), College Park, MD 20742-3360, USA. dayie@umd.edu
Thakur
Chandar S
CS
D000645:Ammonium Sulfate / Q000737:chemistry / Q000378:metabolism; D002247:Carbon Isotopes / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005561:Formates / Q000737:chemistry / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D008968:Molecular Conformation; D009587:Nitrogen Isotopes / Q000737:chemistry / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009711:Nucleotides / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D010427:Pentose Phosphate Pathway; D012313:RNA / Q000096:biosynthesis* / Q000737:chemistry
D002247:Carbon Isotopes; D005561:Formates; D009587:Nitrogen Isotopes; D009711:Nucleotides; C030544:formic acid; D012313:RNA; D005954:Glucosephosphate Dehydrogenase; D000645:Ammonium Sulfate