A novel fluorescent-based assay reveals that thrombopoietin signaling and Bcl-X(L) influence, respectively, platelet and erythrocyte lifespans.
Coupland
Lucy A
LA
The John Curtin School of Medical Research, The Australian National University, Canberra, ACT 2601, Australia.
Cromer
Deborah
D
Davenport
Miles P
MP
Parish
Christopher R
CR
D000818:Animals; D001792:Blood Platelets / Q000166:cytology*; D016922:Cellular Senescence; D004912:Erythrocytes / Q000166:cytology*; D005434:Flow Cytometry; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D015398:Signal Transduction; D013388:Succinimides / Q000737:chemistry*; D013926:Thrombopoietin / Q000378:metabolism*; D051020:bcl-X Protein / Q000378:metabolism*
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013388:Succinimides; D051020:bcl-X Protein; D013926:Thrombopoietin