Calorimetric and diffractometric evidence for the sequential crystallization of buffer components and the consequential pH swing in frozen solutions.
Sundaramurthi
Prakash
P
Department of Pharmaceutics, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Shalaev
Evgenyi
E
Suryanarayanan
Raj
R
D002021:Buffers; D002152:Calorimetry, Differential Scanning; D003080:Cold Temperature; D003460:Crystallization; D006863:Hydrogen-Ion Concentration; D012996:Solutions; D019802:Succinic Acid / Q000737:chemistry*; D017356:Synchrotrons; D014961:X-Ray Diffraction
D002021:Buffers; D012996:Solutions; D019802:Succinic Acid