title: Comparative in vitro stimulation with lipopolysaccharide to study TNFalpha gene expression in fresh whole blood, fresh and frozen peripheral blood mononuclear cells.
authors.affiliation: Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA. jiwang@northwestern.edu <jiwang@northwestern.edu>
authors.forename: Jiwang
authors.initials: J
authors.lastname: Chen
authors.forename: Anke H
authors.initials: AH
authors.lastname: Bruns
authors.forename: Helen K
authors.initials: HK
authors.lastname: Donnelly
authors.forename: Richard G
authors.initials: RG
authors.lastname: Wunderink
mesh_terms: D000328:Adult; D002470:Cell Survival; D015925:Cryopreservation; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha