Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
Vandenbussche
Frank
F
Department of Virology, Veterinary Agrochemical Research Center, Ukkel, Belgium, frvan@var.fgov.be.
Vandemeulebroucke
Elise
E
De Clercq
Kris
K
D000818:Animals; D001820:Bluetongue virus / Q000235:genetics* / Q000302:isolation & purification*; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis*; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity
D012367:RNA, Viral; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)