High-yield expression of human vascular endothelial growth factor VEGF(165) in Escherichia coli and purification for therapeutic applications.
Pizarro
Shelly A
SA
Genentech, Inc., Process Research and Development, 1 DNA Way, South San Francisco, CA 94080, USA. spizarro@gene.com
Gunson
Jane
J
Field
Matthew J
MJ
Dinges
Rachel
R
Khoo
Stefanie
S
Dalal
Milind
M
Lee
Michael
M
Kaleas
Kimberly A
KA
Moiseff
Kathryn
K
Garnick
Susan
S
Reilly
Dorothea E
DE
Laird
Michael W
MW
Schmelzer
Charles H
CH
D001120:Arginine / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D002479:Inclusion Bodies / Q000737:chemistry; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012685:Sepharose / Q000031:analogs & derivatives / Q000737:chemistry; D014508:Urea / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification
D011994:Recombinant Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C020927:phenyl-sepharose; D014508:Urea; D012685:Sepharose; D001120:Arginine