title: High-yield expression of human vascular endothelial growth factor VEGF(165) in Escherichia coli and purification for therapeutic applications.
authors.affiliation: Genentech, Inc., Process Research and Development, 1 DNA Way, South San Francisco, CA 94080, USA. spizarro@gene.com
authors.forename: Shelly A
authors.initials: SA
authors.lastname: Pizarro
authors.forename: Jane
authors.initials: J
authors.lastname: Gunson
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Field
authors.forename: Rachel
authors.initials: R
authors.lastname: Dinges
authors.forename: Stefanie
authors.initials: S
authors.lastname: Khoo
authors.forename: Milind
authors.initials: M
authors.lastname: Dalal
authors.forename: Michael
authors.initials: M
authors.lastname: Lee
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Kaleas
authors.forename: Kathryn
authors.initials: K
authors.lastname: Moiseff
authors.forename: Susan
authors.initials: S
authors.lastname: Garnick
authors.forename: Dorothea E
authors.initials: DE
authors.lastname: Reilly
authors.forename: Michael W
authors.initials: MW
authors.lastname: Laird
authors.forename: Charles H
authors.initials: CH
authors.lastname: Schmelzer
mesh_terms: D001120:Arginine / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D002479:Inclusion Bodies / Q000737:chemistry; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012685:Sepharose / Q000031:analogs & derivatives / Q000737:chemistry; D014508:Urea / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C020927:phenyl-sepharose; D014508:Urea; D012685:Sepharose; D001120:Arginine