Detection and quantification of Panton-Valentine leukocidin in Staphylococcus aureus cultures by ELISA and Western blotting: diethylpyrocarbonate inhibits binding of protein A to IgG.
Nguyen
Hien M
HM
Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California, United States.
Rocha
Miguel A
MA
Chintalacharuvu
Koteswara R
KR
Beenhouwer
David O
DO
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western / Q000379:methods*; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005098:Exotoxins / Q000032:analysis* / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology* / Q000378:metabolism; D007956:Leukocidins / Q000032:analysis* / Q000276:immunology; D051379:Mice; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000276:immunology
D001427:Bacterial Toxins; D005098:Exotoxins; D007074:Immunoglobulin G; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D013205:Staphylococcal Protein A; D004047:Diethyl Pyrocarbonate