Cytotoxic effects of G(M1) ganglioside and amyloid β-peptide on mouse embryonic neural stem cells.
Yanagisawa
Makoto
M
Institute of Molecular Medicine and Genetics and Institute of Neuroscience, Medical College of Georgia, Augusta, GA 30912, USA.
Ariga
Toshio
T
Yu
Robert K
RK
D000544:Alzheimer Disease / Q000473:pathology / Q000503:physiopathology; D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019070:Cell Lineage; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010446:Peptide Fragments / Q000494:pharmacology*
D016229:Amyloid beta-Peptides; D015415:Biomarkers; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); D005677:G(M1) Ganglioside
AD, Alzheimer’s disease; Alzheimer’s disease (AD); Aβ, amyloid β-peptide; CCD, charge-coupled device; DMEM, Dulbecco’s modified Eagle’s medium; ERK, extracellular-signal-regulated kinase; FITC-Aβ-(1–40), FITC-conjugated Aβ-(1–40); GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GM1 ganglioside; IACUC, Institutional Animal Care and Use Committee; IL, interleukin; MAP2, microtubule-associated protein 2; MAPK, mitogen-activated protein kinase; N2-DMEM/F12, N2-supplemented DMEM/Ham’s Nutrient Mixture F12; NEC, neuroepithelial cell; NSC, neural stem cell; RT–PCR, reverse transcription–PCR; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling; amyloid β-peptide (Aβ); apoptosis; bFGF, basic fibroblast growth factor; biotin-Ctxb, biotin-conjugated cholera toxin B subunit; glycosphingolipid; neural stem cell