title: Expression and purification of ataxin-1 protein.
authors.affiliation: Van Cleef Roet Centre for Nervous Diseases, Monash University (AMREP), The Alfred Hospital, Commercial Road, Melbourne, VIC 3004, Australia. eponnampalam@dairyinnovation.com.au
authors.forename: Rhonda
authors.initials: R
authors.lastname: Husain-Ponnampalam
authors.forename: Victor
authors.initials: V
authors.lastname: Turnbull
authors.forename: Volga
authors.initials: V
authors.lastname: Tarlac
authors.forename: Elsdon
authors.initials: E
authors.lastname: Storey
mesh_terms: D000345:Affinity Labels / Q000737:chemistry*; D000595:Amino Acid Sequence / Q000502:physiology; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D000067696:Ataxin-1; D000067528:Ataxins; D001671:Biochemistry / Q000379:methods; D001681:Biological Assay / Q000379:methods*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009419:Nerve Tissue Proteins / Q000302:isolation & purification* / Q000378:metabolism; D009687:Nuclear Proteins / Q000302:isolation & purification* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis*
chemical_list: C000593749:ATXN1 protein, human; D000345:Affinity Labels; D000911:Antibodies, Monoclonal; D000067696:Ataxin-1; D000067528:Ataxins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D010450:Endopeptidases; C099488:TEV protease