title: Development and optimisation of a duplex real-time reverse transcription quantitative PCR assay targeting the VP7 and NS2 genes of African horse sickness virus.
authors.affiliation: Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa. melvyn.quan@up.ac.za
authors.forename: M
authors.initials: M
authors.lastname: Quan
authors.forename: C W
authors.initials: CW
authors.lastname: Lourens
authors.forename: N J
authors.initials: NJ
authors.lastname: MacLachlan
authors.forename: I A
authors.initials: IA
authors.lastname: Gardner
authors.forename: A J
authors.initials: AJ
authors.lastname: Guthrie
mesh_terms: D000355:African Horse Sickness / Q000175:diagnosis* / Q000821:virology; D000356:African Horse Sickness Virus / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D001769:Blood / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D006736:Horses; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014758:Viral Core Proteins / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; D017931:DNA Primers; D012367:RNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; C069264:VP7 major core antigen, African horsesickness virus