Co-expression of ferrochelatase allows for complete heme incorporation into recombinant proteins produced in E. coli.
Sudhamsu
Jawahar
J
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.
Kabir
Mariam
M
Airola
Michael V
MV
Patel
Bhumit A
BA
Yeh
Syun-Ru
SR
Rousseau
Denis L
DL
Crane
Brian R
BR
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005294:Ferrochelatase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006418:Heme / Q000378:metabolism*; D006420:Hemeproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013057:Spectrum Analysis; D013059:Spectrum Analysis, Raman
D001426:Bacterial Proteins; D002352:Carrier Proteins; D006420:Hemeproteins; D011993:Recombinant Fusion Proteins; D006418:Heme; D006639:Histidine; D003577:Cytochrome P-450 Enzyme System; D019001:Nitric Oxide Synthase; D005294:Ferrochelatase