title: Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
authors.affiliation: Department of Virology, Veterinary Agrochemical Research Center, Ukkel, Belgium, frvan@var.fgov.be.
authors.forename: Frank
authors.initials: F
authors.lastname: Vandenbussche
authors.forename: Elise
authors.initials: E
authors.lastname: Vandemeulebroucke
authors.forename: Kris
authors.initials: K
authors.lastname: De Clercq
mesh_terms: D000818:Animals; D001820:Bluetongue virus / Q000235:genetics* / Q000302:isolation & purification*; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis*; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)