Synthesis of human renalase1 in Escherichia coli and its purification as a FAD-containing holoprotein.
Pandini
Vittorio
V
Dipartimento di Scienze Biomolecolari e Biotecnologie, Università degli Studi di Milano, via Celoria 26, 20133 Milano, Italy.
Ciriello
Francesco
F
Tedeschi
Gabriella
G
Rossoni
Giuseppe
G
Zanetti
Giuliana
G
Aliverti
Alessandro
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002846:Chromatography, Affinity; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry / Q000378:metabolism*; D006439:Hemodynamics / Q000187:drug effects; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D008995:Monoamine Oxidase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism; D012694:Serine / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011994:Recombinant Proteins; D025842:SUMO-1 Protein; D005182:Flavin-Adenine Dinucleotide; C033223:polyhistidine; D012694:Serine; D006639:Histidine; D008995:Monoamine Oxidase; C521557:renalase