Normalizing RT-qPCR data: are we getting the right answers? An appraisal of normalization approaches and internal reference genes from a case study in the finfish Lates calcarifer.
De Santis
Christian
C
Aquaculture Genetics Research Program, School of Marine and Tropical Biology, James Cook University, Townsville, 4811, QLD, Australia. Christian.desantis@jcu.edu.au
Smith-Keune
Carolyn
C
Jerry
Dean R
DR
D000818:Animals; D005215:Fasting; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism / Q000592:standards; D005508:Food Deprivation; D015870:Gene Expression / Q000502:physiology; D010473:Perciformes / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards*
D029941:Fish Proteins; D012333:RNA, Messenger