Integration of PEGylation and refolding for renaturation of recombinant proteins from insoluble aggregates produced in bacteria--application to a single-chain Fv fragment.
Kumagai
Izumi
I
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba 6-6-11-607, Aramaki, Aoba-ku, Sendai 980-8579, Japan. kmiz@kuma.che.tohoku.ac.jp
Asano
Ryutaro
R
Nakanishi
Takeshi
T
Hashikami
Kentaro
K
Tanaka
Sho
S
Badran
Adel
A
Sanada
Hideaki
H
Umetsu
Mitsuo
M