title: Oxidized regenerated cellulose does not prevent the formation of experimental postoperative perineural fibrosis assessed by digital analysis.
abstract: It is difficult to prevent and treat intra- and peri-neural fibrosis after peripheral nerve surgery. Many authors have attempted to develop and verify the effectiveness of substances to decrease the formation of adherences in different tissues.
  
  
  
  this study aimed to assess the effectiveness of a barrier of oxidized regenerated cellulose (ORC) to reduce adherence and perineural fibrosis in a model of surgical perineural induced fibrosis in rat sciatic nerve in 40 rats. After tissue aggression, the nerve of the right rear limb was wrapped in ORC and the left limb served as control. Animals were killed at 3 and 6 weeks, and nerves and muscle mass were extracted en bloc. Connective tissue was quantified by conventional histopathological techniques and Fibrosis HR(R) automatic image analysis.
  
  
  
  No significant differences were found in intra- or peri-neural induced fibrosis between control nerves (6.88% and 8.90%, respectively) and treated nerves (6.57% and 9.90%) at 3 or 6 weeks (10.41% and 12.51% in controls; 11.85% and 15.72% in treated nerves). Inflammatory phenomena and granulomatous reactions were more frequent in treated animals.
  
  
  
  ORC conferred no advantage in prevention of nerve fibrosis and might have interfered with healing.
authors.affiliation: Department of Traumatology and Orthopaedic Surgery, IBIMER, "San Cecilio" Clinical Hospital and University of Granada, Spain. hdezcp@hotmail.com
authors.forename: Pedro
authors.initials: P
authors.lastname: Hernández-Cortés
authors.forename: Magdalena
authors.initials: M
authors.lastname: Peregrina
authors.forename: Jose
authors.initials: J
authors.lastname: Aneiros-Fernández
authors.forename: Mohamed
authors.initials: M
authors.lastname: Tassi
authors.forename: Miguel
authors.initials: M
authors.lastname: Pajares-López
authors.forename: Miguel
authors.initials: M
authors.lastname: Toledo
authors.forename: Francisco
authors.initials: F
authors.lastname: O'Valle
mesh_terms: D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives*; D002483:Cellulose, Oxidized / Q000494:pharmacology*; D004195:Disease Models, Animal; D005355:Fibrosis / Q000473:pathology / Q000517:prevention & control*; D015745:Granuloma, Foreign-Body / Q000139:chemically induced / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects* / Q000293:injuries / Q000473:pathology; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control*; D014945:Wound Healing
chemical_list: D002483:Cellulose, Oxidized; D002482:Cellulose