title: Nanoscale sensor design via in situ labeling of gold nanoparticles onto protein scaffolds.
abstract: A one-step method for synthesis of bioconjugated gold nanoparticles is reported. A non-toxic and biocompatible phosphorus based reducing agent was used for reduction of gold (III) and formation of nanoparticles. Physicochemical properties of protein-A stabilized gold nanoparticls were investigated. Result of immunoassay experiments confirmed the potential of the synthesized anti-protein-A conjugated gold nanoparticles for use as a simple and inexpensive test for quantitative screening of protein-A samples.
authors.affiliation: Department of Life and Physical Sciences, Lincoln University, Jefferson City, MO 65101, USA.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Afrasiabi
authors.forename: Ravi
authors.initials: R
authors.lastname: Shukla
authors.forename: Nripen
authors.initials: N
authors.lastname: Chanda
authors.forename: Sharanya
authors.initials: S
authors.lastname: Bhaskaran
authors.forename: Anandhi
authors.initials: A
authors.lastname: Upendran
authors.forename: Ajit
authors.initials: A
authors.lastname: Zambre
authors.forename: Kattesh V
authors.initials: KV
authors.lastname: Katti
authors.forename: Raghuraman
authors.initials: R
authors.lastname: Kannan
mesh_terms: D006046:Gold; D053768:Metal Nanoparticles; D036103:Nanotechnology; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D006046:Gold