title: [Evaluation of the GenoType MTBDRplus assay for rifampin and isoniazid susceptibility testing of Mycobacterium tuberculosis].
abstract: OBJECTIVE
  to evaluate the ability of GenoType MTBDRplus Assay for detection of rifampin (RFP) and isoniazid (INH) resistance in Mycobacterium tuberculosis.
  
  
  METHODS
  seventy-five clinical isolates were tested for traditional drug susceptibility testing and GenoType MTBDRplus assay. The results were compared to assess the sensitivity and specificity of GenoType MTBDRplus assay.
  
  
  RESULTS
  the sensitivity of GenoType MTBDRplus assays for detection of RFP and INH resistance were 98.0% and 86.5%, respectively. In pan-susceptible isolates of Mycobacterium tuberculosis, the specificity was 100% for both RFP resistance and INH resistance.
  
  
  CONCLUSIONS
  with its high sensitivity and specificity, GenoType MTBDRplus assay is a promising rapid method to detect INH and RFP resistant Mycobacterium tuberculosis strains in Shanghai.
authors.affiliation: Department of Tuberculosis, Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Gui
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Gao
authors.forename: Jian
authors.initials: J
authors.lastname: Mei
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D012293:Rifampin / Q000494:pharmacology; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D011933:Reagent Kits, Diagnostic; D007538:Isoniazid; D012293:Rifampin