title: Effect of inoculated corn silage enriched with sunflower oil on rumen fermentation and lipid metabolism in an artificial rumen (RUSITEC).
abstract: BACKGROUND
  Some rumen isolates are able to produce conjugated linoleic acid (CLA) from linoleic acid (LA) in vitro. Effects of providing diets containing corn silage (CS) and lucerne hay to an artificial rumen (RUSITEC) in which the corn was not inoculated (CS), or inoculated with Lactobacillus plantarum CCM 4000 (CS + LP), Lactobacillus fermentum LF2 (CS + LF) or Enterococcus faecium CCM 4231 (CS + EF) and supplied with sunflower oil (SO; 30 g kg(-1); w/w) on rumen metabolism were examined.
  
  
  RESULTS
  The SO affected the outputs of all fatty acids. TVA output of uninoculated CS with SO was lower as compared to inoculated CS. The interaction of the CS x SO in the daily output of TVA was detected (P < 0.001). The biohydrogenation of oleic, linoleic, alpha-linolenic and total fatty acids was influenced by SO (P < 0.05 and P < 0.001).
  
  
  CONCLUSIONS
  Inoculated silage induces changes in the rumen metabolism which might be related to differences observed in the extent of rumen BH of PUFA in RUSITEC. SO supplementation might positively enhances the production of some rumen intermediates; however, relationships between inoculated silages and oil supplementation can be presumed in the daily production of trans-vaccenic acid.
authors.affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, Soltésovej 4-6, 040 01 Kosice, Slovak Republic. jalcd@saske.sk
authors.forename: Dusan
authors.initials: D
authors.lastname: Jalc
authors.forename: Zora
authors.initials: Z
authors.lastname: Váradyová
authors.forename: Andrea
authors.initials: A
authors.lastname: Lauková
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism / Q000494:pharmacology; D016984:Enterococcus faecium / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism / Q000494:pharmacology; D005285:Fermentation; D006865:Hydrogenation; D048191:Lactiplantibacillus plantarum / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D000455:Medicago sativa; D023421:Models, Animal; D009829:Oleic Acids / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism / Q000494:pharmacology; D012417:Rumen / Q000378:metabolism* / Q000382:microbiology; D012820:Silage / Q000382:microbiology*; D000074242:Sunflower Oil; D003313:Zea mays / Q000382:microbiology*
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D009829:Oleic Acids; D010938:Plant Oils; D000074242:Sunflower Oil; C050413:11-octadecenoic acid