title: Methyl cyanide induces alpha to beta transition and aggregation at high concentrations in E-state of human serum albumin.
abstract: We have studied the effect of 2,2,2-trifluoroethanol (TFE), an alpha-helix inducer, versus methyl cyanide (MeCN), a beta-sheet inducer, on acid-denatured human serum albumin (HSA) using far-UV circular dichroism, intrinsic fluorescence, 1-anilino-8-naphthalene sulfonate binding, and acrylamide quenching studies. Interestingly, at pH 2.0, where the recovery and resolution of the protein in reverse phase chromatography is high, its secondary structure remains unchanged even in the presence of very high concentration (76% v/v) of MeCN. Gain of 23 and 34% alpha-helicity was observed in the presence of 20 and 50% TFE, respectively. At pH 7.3, HSA aggregates in the presence of 40% MeCN, but it remains soluble up to 75% MeCN at pH 2.0. The results seem to be important for HSA isolation and purification.
authors.affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
authors.forename: P
authors.initials: P
authors.lastname: Sen
authors.forename: M A
authors.initials: MA
authors.lastname: Iqbal
authors.forename: S
authors.initials: S
authors.lastname: Fatima
authors.forename: R H
authors.initials: RH
authors.lastname: Khan
mesh_terms: D000097:Acetonitriles / Q000494:pharmacology*; D002942:Circular Dichroism; D006801:Humans; D017433:Protein Structure, Secondary; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014270:Trifluoroethanol / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D012709:Serum Albumin; D014270:Trifluoroethanol