Ketamine anesthesia helps preserve neuronal viability.
The dissociative anesthetic ketamine that acts as an N-methyl-D-aspartate (NMDA) antagonist has been reported to improve neurological damage after experimental ischemic challenges. Here we show that deep anesthesia with ketamine before euthanasia by decapitation improves the quality of neonatal mouse neuronal brain slice preparations. Specifically we found that neurons of the locus coeruleus (LC) and hypoglossal motor neurons had significantly higher input resistances, and LC neurons that generally are difficult to voltage control, could be more reliably voltage clamped compared to control neurons.
de Oliveira
Ramatis B
RB
School of Biomedical Sciences and Pharmacy, University of Newcastle, University Drive, Newcastle, NSW 2308, Australia.
Graham
Brett
B
Howlett
Marcus C H
MC
Gravina
Fernanda S
FS
Oliveira
Max W S
MW
Imtiaz
Mohammad S
MS
Callister
Robert J
RJ
Lim
Rebecca
R
Brichta
Alan M
AM
van Helden
Dirk F
DF
D000758:Anesthesia; D000778:Anesthetics, Dissociative / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival; D025802:Electric Capacitance; D017097:Electric Impedance; D007002:Hypoglossal Nerve / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D007649:Ketamine / Q000008:administration & dosage / Q000494:pharmacology*; D008125:Locus Coeruleus / Q000187:drug effects / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D009926:Organ Preservation / Q000379:methods; D018408:Patch-Clamp Techniques; D020858:Tissue and Organ Harvesting / Q000379:methods*
D000778:Anesthetics, Dissociative; D007649:Ketamine