title: The effect of topology of chitosan biomaterials on the differentiation and proliferation of neural stem cells.
abstract: Neural stem cells (NSCs) are capable of self-renewal and differentiation into three principle central nervous system cell types under specific local microenvironments. Chitosan films (Chi-F), chitosan porous scaffolds (Chi-PS) and chitosan multimicrotubule conduits (Chi-MC) were used to investigate their effects on the differentiation and proliferation of NSCs isolated from the cortices of fetal rats. In the presence of 10% fetal bovine serum most NSCs cultured on Chi-F differentiated into astrocytes, NSCs cultured on Chi-MC showed a significant increase in neuronal differentiation, while Chi-PS somewhat promoted NSCs to differentiate into neurons. However, in serum-free medium with 20 ng ml(-1) basic fibroblast growth factor NSCs cultured on Chi-F showed the greatest proliferation, NSCs cultured on Chi-MC showed moderate cell proliferation, but NSCs cultured on Chi-PS exhibited the least cell proliferation. These observations indicate that chitosan topology can play an important role in regulating differentiation and proliferation of NSCs and raise the possibility of the utilization of chitosan in various structural biomaterials in neural tissue engineering.
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Life Sciences, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Gan
authors.initials: G
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ao
authors.forename: Kai
authors.initials: K
authors.lastname: Gong
authors.forename: Aijun
authors.initials: A
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Zheng
authors.forename: Yandao
authors.initials: Y
authors.lastname: Gong
authors.forename: Xiufang
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D019070:Cell Lineage / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D016895:Culture Media, Serum-Free; D014404:Tubulin; D016222:Fibroblast Growth Factor 2; D048271:Chitosan