[Protective effect of recombinant HMGB1 A box protein in mouse with acute hepatic failure].
OBJECTIVE
To evaluate the effect of recombinant HMGB1 A box protein in mouse with acute hepatic failure.


METHODS
Acute hepatic failure was induced by D-galactosamine and lipopolysaccharide in mice. After injection with saline (control) or recombinant HMGB1 A box proteins, the expression of HMGB1 in liver tissues was detected by immunohistochemistry and RT-PCR, and the serum TNFalpha and IL-1beta were quantified.


RESULTS
rHMGB1-Abox protein alleviated the acute liver damage. rHMGB1-Abox protein treatment decreased the expression of HMGB1 in liver tissues and reduced the serum levels of TNFalpha and IL-1beta.


CONCLUSIONS
rHMGB1-Abox protein can alleviate the acute liver damage and inhibit the expression of HMGB1.
Liu
Ting
T
Department of Infectious Disease, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
He
Yong-Wen
YW
D000818:Animals; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D017114:Liver Failure, Acute / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D011994:Recombinant Proteins / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D024243:HMGB1 Protein; D053583:Interleukin-1beta; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha