[Establishment of a method for rapid detection of the nucleic acid of the novel A (H1N1) influenza virus].
A new flu caused by a novel influenza A(H1N1) virus has spread over the United States, Mexico and more than 40 other countries. And because of the immediate global concern, WHO has announced that the current level of influenza pandemic alert is raised to phase 5, indicating approaching of an influenza pandemic. As patients suffering from the influenza A (H1N1) have the similar symptoms as patients with seasonal influenza, differential detection and identification of the influenza virus have to depend on specific laboratory tests. We have successfully developed a RT-PCR based method for detection of the influenza A (H1N1) virus, and had applied the method to detection of clinical samples.
Wang
Da-Yan
DY
China CDC, Institute for Viral Disease Control and Prevention, Key laboratory for Virus Gene Engineer, Beijing 100052, China. dayanwang@yahoo.com.cn
Gao
Rong-Bao
RB
Li
Xiao-Dan
XD
Wang
Wei
W
Wen
Le-Ying
LY
Zou
Shu-Mei
SM
Zhao
Xiang
X
Li
Xi-Yan
XY
Lan
Yu
Y
Yang
Lei
L
Guo
Jun-Feng
JF
Li
Zi
Z
Cheng
Yan-Hui
YH
Tan
Min-Jju
MJ
Li
Xin-Wan
XW
Zeng
Yu-Hong
YH
Guo
Yuan-Ji
YJ
Li
De-Xin
DX
Shu
Yue-Long
YL
D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D012367:RNA, Viral