title: Experimental xenoimplantation of antlerogenic cells into mandibular bone lesions in rabbits: two-year follow-up.
abstract: Different types of cells require activation, and take part in annual, dynamic growth of deer antlers. Stem cells play the most important role in this process. This report shows the results of a two-year long observation of xenogenic implant of antlerogenic stem cells (cell line MIC-1). The cells were derived from growing antler of a deer (Cervus elaphus), seeded onto Spongostan and placed in postoperative lesions of mandibular bones of 15 experimental rabbits. The healing process observed in the implantation sites in all rabbits was normal, and no local inflammatory response was ever observed. Histological and immunohistochemical evaluations were performed after 1, 2, 6, 12 and 24 months, and confirmed the participation of xenogenic cells in the regeneration processes, as well as a lack of rejection of the implants. The deficiencies in the bones were replaced by newly formed, thick fibrous bone tissue that underwent mineralization and was later remodelled into lamellar bone. The results of the experiment with rabbits allow us to believe that antlerogenic cells could be used in reconstruction of bone tissues in other species as well.
authors.affiliation: Department of Histology and Embryology, Medical University of Wroclaw, 50-368 Wroclaw, Poland. marcel@hist.am.wroc.pl
authors.forename: Marek
authors.initials: M
authors.lastname: Cegielski
authors.forename: Wojciech
authors.initials: W
authors.lastname: Dziewiszek
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziegiel
authors.forename: Jan
authors.initials: J
authors.lastname: Kuryszko
authors.forename: Ilona
authors.initials: I
authors.lastname: Izykowska
authors.forename: Maciej
authors.initials: M
authors.lastname: Zatoński
authors.forename: Marek
authors.initials: M
authors.lastname: Bochnia
mesh_terms: D000818:Animals; D000999:Antlers / Q000166:cytology*; D016723:Bone Remodeling / Q000502:physiology*; D003670:Deer; D005260:Female; D005339:Fibrin Foam / Q000494:pharmacology; D005500:Follow-Up Studies; D017102:Fracture Healing; D006084:Graft Rejection / Q000473:pathology; D006085:Graft Survival; D007150:Immunohistochemistry; D008334:Mandible / Q000000981:diagnostic imaging / Q000473:pathology*; D008336:Mandibular Diseases / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D008854:Microscopy, Electron; D011817:Rabbits; D011859:Radiography; D012038:Regeneration / Q000502:physiology; D033581:Stem Cell Transplantation / Q000379:methods*; D014183:Transplantation, Heterologous / Q000379:methods*; D014463:Ultrasonography
chemical_list: D005339:Fibrin Foam