High-resolution separation of tubulin monomers on polyacrylamide minigels.
High-resolution separation of alpha- and beta-tubulin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on minigels can be performed rapidly using simple modifications of the standard Laemmli procedure. Separation of the subunits can be observed even in high-protein loads (up to 40microg of protein).
Banerjee
Abhijit
A
Department of Chemistry, Binghamton University, NY 13902, USA.
Bovenzi
Frank A
FA
Bane
Susan L
SL
D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000191:economics / Q000379:methods*; D021122:Protein Subunits / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D014404:Tubulin / Q000302:isolation & purification*
D021122:Protein Subunits; D014404:Tubulin; D012967:Sodium Dodecyl Sulfate