Phase microscopy of technical and biological samples through random phase modulation with a diffuser.
A technique for phase microscopy using a phase diffuser and a reconstruction algorithm is proposed. A magnified specimen wavefront is projected on the diffuser plane that modulates the wavefront into a speckle field. The speckle patterns at axially displaced planes are sampled and used in an iterative phase retrieval algorithm based on a wave-propagation equation. The technique offers a whole-field and high-resolution wavefront reconstruction of unstained microstructures. Phase maps of photoresist targets and human cheek cells are obtained to demonstrate the effectiveness of our method.
Almoro
Percival F
PF
DTU Fotonik, Department of Photonics Engineering, Danmarks Tekniske Universitet, Roskilde 4000, Denmark. pfalmoro@gmail.com
Pedrini
Giancarlo
G
Gundu
Phanindra N
PN
Osten
Wolfgang
W
Hanson
Steen G
SG
D000465:Algorithms; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007909:Lenses; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation* / Q000379:methods*; D012031:Refractometry / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity