Reduction of the immunogenicity of beta-lactoglobulin from cow's milk by conjugation with a dextran derivative.
Beta-lactoglobulin (BLG) was conjugated with the N-hydroxysuccinimide ester of the dextran-glycylglycine adduct (DG-ONSu) to reduce the immunogenicity of BLG, a major allergen of cow's milk, and some immunological properties of the conjugate (DG-BLG) were studied. The conjugate was prepared by modifying BLG with DG-ONSu and purified in a Sephadex G-100 column. The analytical data for DG-BLG indicated that 5.2 moles of DG-ONSu with a mean molecular weight of 9,300 were covalently attached to the amino groups of the BLG molecule. Conjugation with DG-ONSu greatly decreased the reactivity of BLG with anti-BLG antibodies and suppressed their production in vivo due to its shielding action for epitope(s) on the protein's molecular surface. It was also found that DG-BLG was resistant to proteolytic enzymes. These findings allow us to suggest that DG-ONSu could be advantageously used to suppress the hypersensitivity mediated by IgG antibodies in milk allergy.
Nodake
Yuichi
Y
Department of Pharmacy, Faculty of Pharmaceutical Sciences, Nagasaki International University, Japan.
Fukumoto
Shoichi
S
Fukasawa
Masashi
M
Sakakibara
Ryuzo
R
Yamasaki
Nobuyuki
N
D000485:Allergens / Q000276:immunology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D002417:Cattle; D003911:Dextrans / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006967:Hypersensitivity / Q000276:immunology*; D007109:Immunity / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007782:Lactoglobulins / Q000276:immunology* / Q000378:metabolism; D008892:Milk / Q000276:immunology / Q000378:metabolism; D016269:Milk Hypersensitivity / Q000276:immunology*; D013388:Succinimides
D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D003911:Dextrans; D000939:Epitopes; D007074:Immunoglobulin G; D007782:Lactoglobulins; D013388:Succinimides; D007073:Immunoglobulin E; C025614:sephadex; C001426:N-hydroxysuccinimide