Impact of nanoscale zero valent iron on geochemistry and microbial populations in trichloroethylene contaminated aquifer materials.
Nanoscale zerovalent iron (NZVI) particles are a promising technology for reducing trichloroethylene (TCE) contamination in the subsurface. Prior to injecting large quantities of nanoparticles into the groundwater it is important to understand what impact the particles will have on the geochemistry and indigenous microbial communities. Microbial populations are important not only for nutrient cycling, but also for contaminant remediation and heavy metal immobilization. Microcosms were used to determine the effects of NZVI addition on three different aquifer materials from TCE contaminated sites in Alameda Point, CA, Mancelona, MI, and Parris Island, SC. The oxidation and reduction potential of the microcosms consistently decreased by more than 400 mV when NZVI was added at 1.5 g/L concentrations. Sulfate concentrations decreased in the two coastal aquifer materials, and methane was observed in the presence of NZVI in Alameda Point microcosms, but not in the other two materials. Denaturing gradient gel electrophoresis (DGGE) showed significant shifts in Eubacterial diversity just after the Fe(0) was exhausted, and quantitative polymerase chain reaction (qPCR) analyses showed increases of the dissimilatory sulfite reductase gene (dsrA) and Archaeal 16s rRNA genes, indicating that reducing conditions and hydrogen created by NZVI stimulate both sulfate reducer and methanogen populations. Adding NZVI had no deleterious effect on total bacterial abundance in the microcosms. NZVI with a biodegradable polyaspartate coating increased bacterial populations by an order of magnitude relative to controls. The lack of broad bactericidal effect, combined with the stimulatory effect of polyaspartate coatings, has positive implications for NZVI field applications.
Kirschling
Teresa L
TL
Department of Chemical Engineering, Center for the Environmental Implications of NanoTechnology (CEINT), Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.
Gregory
Kelvin B
KB
Minkley
Edwin G
EG
Lowry
Gregory V
GV
Tilton
Robert D
RD
D002621:Chemistry / Q000379:methods; D004566:Electrodes; D004586:Electrophoresis / Q000379:methods; D005844:Geology / Q000379:methods; D006859:Hydrogen / Q000737:chemistry; D007501:Iron / Q000737:chemistry*; D019216:Metals, Heavy / Q000737:chemistry; D008956:Models, Chemical; D036103:Nanotechnology / Q000379:methods; D010100:Oxygen / Q000737:chemistry; D016133:Polymerase Chain Reaction; D013431:Sulfates / Q000737:chemistry; D014241:Trichloroethylene / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D018508:Water Purification / Q000379:methods
D019216:Metals, Heavy; D013431:Sulfates; D014874:Water Pollutants, Chemical; D014241:Trichloroethylene; D006859:Hydrogen; D007501:Iron; D010100:Oxygen