Super-SILAC mix for quantitative proteomics of human tumor tissue.
We describe a method to accurately quantify human tumor proteomes by combining a mixture of five stable-isotope labeling by amino acids in cell culture (SILAC)-labeled cell lines with human carcinoma tissue. This generated hundreds of thousands of isotopically labeled peptides in appropriate amounts to serve as internal standards for mass spectrometry-based analysis. By decoupling the labeling from the measurement, this super-SILAC method broadens the scope of SILAC-based proteomics.
Geiger
Tamar
T
Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany.
Cox
Juergen
J
Ostasiewicz
Pawel
P
Wisniewski
Jacek R
JR
Mann
Matthias
M
D001254:Astrocytoma / Q000737:chemistry; D001943:Breast Neoplasms / Q000737:chemistry*; D002247:Carbon Isotopes; D045744:Cell Line, Tumor; D005260:Female; D005909:Glioblastoma / Q000737:chemistry; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D009363:Neoplasm Proteins / Q000032:analysis*; D009587:Nitrogen Isotopes; D040901:Proteomics / Q000379:methods*
D002247:Carbon Isotopes; D009363:Neoplasm Proteins; D009587:Nitrogen Isotopes