PEG10 is a probable target for the amplification at 7q21 detected in hepatocellular carcinoma.
DNA copy number aberrations in human hepatocellular carcinoma (HCC) cell lines were investigated using a high-density oligonucleotide microarray, and a novel amplification at the chromosomal region 7q21 was detected. Molecular definition of the amplicon indicated that PEG10 (paternally expressed gene 10), a paternally expressed imprinted gene, was amplified together with CDK14 (cyclin-dependent kinase 14; previously PFTAIRE protein kinase 1, PFTK1) and CDK6 (cyclin-dependent kinase 6). An increase in PEG10 copy number was detected in 14 of 34 primary HCC tumors (41%). PEG10, but not CDK14 or CDK6, was significantly overexpressed in 30 of 41 tumors (73%) from HCC patients, compared with their nontumorous counterparts. These results suggest that PEG10 is a probable target, acting as a driving force for amplification of the 7q21 region, and may therefore be involved in the development or progression of HCCs.
Tsuji
Kazuhiro
K
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan.
Yasui
Kohichiroh
K
Gen
Yasuyuki
Y
Endo
Mio
M
Dohi
Osamu
O
Zen
Keika
K
Mitsuyoshi
Hironori
H
Minami
Masahito
M
Itoh
Yoshito
Y
Taniwaki
Masafumi
M
Tanaka
Shinji
S
Arii
Shigeki
S
Okanoue
Takeshi
T
Yoshikawa
Toshikazu
T
D000328:Adult; D000368:Aged; D051017:Apoptosis Regulatory Proteins; D016203:CDC2 Protein Kinase / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D051361:Cyclin-Dependent Kinase 6 / Q000235:genetics; D018844:Cyclin-Dependent Kinases / Q000235:genetics; D004268:DNA-Binding Proteins; D018450:Disease Progression; D005260:Female; D005784:Gene Amplification / Q000502:physiology; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000235:genetics*; D016601:RNA-Binding Proteins; D015854:Up-Regulation
D051017:Apoptosis Regulatory Proteins; D004268:DNA-Binding Proteins; C432965:PEG10 protein, human; D011506:Proteins; D016601:RNA-Binding Proteins; D016203:CDC2 Protein Kinase; C515076:CDK14 protein, human; C495911:CDK6 protein, human; D051361:Cyclin-Dependent Kinase 6; D018844:Cyclin-Dependent Kinases