New capillary coatings in open tubular CEC as models for biological membranes.
Novel stationary phases in open tubular CEC were investigated. The coating procedure was fast and simple. The coating material contained membrane suspension of different neuronal cell lines. The performance and stability of three cell lines: human neuroblastoma SH-SY5Y, murine microglia Bv-2 and human glioma U87-MG cells were studied. The coating solution was expected to contain both membrane proteins and membrane lipids. The presence of membrane proteins was tested by Western blotting and the presence of phospholipids by the analysis of phosphorus content. The stability of the coating was estimated by monitoring the mobility of EOF over successive runs. The effects of pH, storage time and temperature on the coating stability were also studied. The results showed that the cell membrane-based coating was stable over pH range of 6.5-8.5. Coatings derived from different cells yielded similar stability and EOF mobility. Capillary coated with a membrane solution was stable over 3-day period. The same coating solution could be used for 3 weeks.
Martma
Kert
K
Department of Natural Sciences, Institute of Mathematics and Natural Sciences, Tallinn University, Tallinn, Estonia.
Fernaeus
Sandra Zetterström
SZ
Land
Tiit
T
Shimmo
Ruth
R
D000818:Animals; D015153:Blotting, Western; D053801:Capillary Electrochromatography / Q000379:methods*; D002460:Cell Line; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry; D053841:Electroosmosis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008563:Membrane Lipids / Q000737:chemistry / Q000302:isolation & purification; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification; D008567:Membranes, Artificial; D051379:Mice; D008954:Models, Biological; D009474:Neurons / Q000737:chemistry / Q000166:cytology*; D055550:Protein Stability; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors
D008563:Membrane Lipids; D008565:Membrane Proteins; D008567:Membranes, Artificial