Kinetics of D-lactic acid production by Sporolactobacillus sp. strain CASD using repeated batch fermentation.
D-lactic acid was produced by Sporolactobacillus sp. strain CASD in repeated batch fermentation with one- and two-reactor systems. The strain showed relatively high energy consumption in its growth-related metabolism in comparison with other lactic acid producers. When the fermentation was repeated with 10% (v/v) of previous culture to start a new batch, D-lactic acid production shifted from being cell-maintenance-dependent to cell-growth-dependent. In comparison with the one-reactor system, D-lactic acid production increased approximately 9% in the fourth batch of the two-reactor system. Strain CASD is an efficient D-lactic acid producer with increased growth rate at the early stage of repeated cycles, which explains the strain's physiological adaptation to repeated batch culture and improved performance in the two-reactor fermentation system. From a kinetic point of view, two-reactor fermentation system was shown to be an alternative for conventional one-reactor repeated batch operation.
Zhao
Bo
B
Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China.
Wang
Limin
L
Li
Fengsong
F
Hua
Dongliang
D
Ma
Cuiqing
C
Ma
Yanhe
Y
Xu
Ping
P
D018533:Biomass; D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005285:Fermentation; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism*; D007778:Lactobacillus / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D019344:Lactic Acid