GC-MS, FT-IR analysis of black fungus polysaccharides and its inhibition against skin aging in mice.
The monosaccharide compositions, molecular weights, structure were investigated for the black fungus polysaccharides. Analysis of sugar components by gas chromatograph-mass spectrometer (GC-MS) showed that component ratios of neutral sugars in the polysaccharides were Glc:Man=82.97:12.93. Fourier transform infrared (FT-IR) analysis showed that the band at about 1649cm(-1) can indicate the presence of CO groups or CC groups vibration in structures. Pharmacological experiment showed that black fungus polysaccharides administration could significantly reduce malondialchehyche (MDA), lipofuscin (LF) contents and enhance superoxide dismutase (SOD) activities in skin. This indicated that black fungus polysaccharides may slow down skin aging.
Peng
Xue-Biao
XB
Department of Dermatology, NanFang Hospital, Southern Medical University, Guangzhou 510515, PR China. pengxb@fimmu.com
Li
Qian
Q
Ou
Li-Na
LN
Jiang
Li-Fen
LF
Zeng
Kang
K
D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006909:Hydroxyproline / Q000378:metabolism; D008062:Lipofuscin / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D012867:Skin / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects*; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000378:metabolism
D008062:Lipofuscin; D011134:Polysaccharides; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D006909:Hydroxyproline