title: Debridement of cartilage lesions before autologous chondrocyte implantation by open or transarthroscopic techniques: a comparative study using post-mortem materials.
abstract: We compared the quality of debridement of chondral lesions performed by four arthroscopic (SH, shaver; CU, curette; SHCU, shaver and curette; BP, bipolar electrodes) and one open technique (OPEN, scalpel and curette) which are used prior to autologous chondrocyte implantation (ACI). The ex vivo simulation of all five techniques was carried out on six juvenile equine stifle joints. The OPEN, SH and SHCU techniques were tested on knees harvested from six adult human cadavers. The most vertical walls with the least adjacent damage to cartilage were obtained with the OPEN technique. The CU and SHCU methods gave inferior, but still acceptable results whereas the SH technique alone resulted in a crater-like defect and the BP method undermined the cartilage wall. The subchondral bone was severely violated in all the equine samples which might have been peculiar to this model. The predominant depth of the debridement in the adult human samples was at the level of the calcified cartilage. Some minor penetrations of the subchondral end-plate were induced regardless of the instrumentation used. Our study suggests that not all routine arthroscopic instruments are suitable for the preparation of a defect for ACI. We have shown that the preferred debridement technique is either open or arthroscopically-assisted manual curettage. The use of juvenile equine stifles was not appropriate for the study of the cartilage-subchondral bone interface.
authors.affiliation: Department of Orthopaedic Surgery, University Medical Centre, Zaloska ulica 9, SI-1000 Ljubljana, Slovenia. matej.drobnic@mf.uni-lj.si
authors.forename: M
authors.initials: M
authors.lastname: Drobnic
authors.forename: D
authors.initials: D
authors.lastname: Radosavljevic
authors.forename: A
authors.initials: A
authors.lastname: Cör
authors.forename: M
authors.initials: M
authors.lastname: Brittberg
authors.forename: K
authors.initials: K
authors.lastname: Strazar
mesh_terms: D000328:Adult; D000818:Animals; D001182:Arthroscopy / Q000379:methods; D002358:Cartilage, Articular / Q000293:injuries* / Q000473:pathology / Q000601:surgery*; D019902:Chondrocytes / Q000637:transplantation*; D003475:Curettage / Q000379:methods; D003646:Debridement / Q000295:instrumentation / Q000379:methods*; D004195:Disease Models, Animal; D006736:Horses; D006801:Humans; D008297:Male; D008875:Middle Aged; D013045:Species Specificity; D013264:Stifle / Q000601:surgery