The production and immunostimulatory activity of double-stranded CpG-DNA.
CpG-DNA, which contains unmethylated CpG dinucleotides in the context of specific sequences, has remarkable and diverse immunological effects, including induction of proinflammatory cytokine expression and regulation of the Th1/Th2 immune response. Here, we examined the immunostimulatory activities of double-stranded (ds) CpG-DNA in the human B cell line RPMI8226. To investigate whether dsCpG-DNA stimulates immune cells, we constructed a plasmid containing repeated dsCpG-DNA and produced dsCpG-DNA by PCR amplification and EcoR I digestion. PCR-amplified dsCpG-DNA alone did not have immmunostimulatory activity. However, dsCpGDNA encapsulated with lipofectin induced IL-8 promoter activation, HLA-DRA expression, and IL-8 expression in a CG sequence- independent manner. The effects of encapsulated dsCpGDNA were independent of minor endotoxin contamination. These findings suggest the potential use of dsCpG-DNA as a therapy for immune response regulation.
Park
Byoung Kwon
BK
Department of Microbiology, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
Kim
Dongbum
D
Rhee
Jae Won
JW
Kim
Min Soo
MS
Seok
Hyun Jeong
HJ
Choi
Soo Young
SY
Park
Jinseu
J
Lee
Younghee
Y
Kwon
Hyung Joo
HJ
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D018899:CpG Islands; D004247:DNA / Q000737:chemistry; D006684:HLA-DR Antigens / Q000235:genetics / Q000378:metabolism; D059805:HLA-DR alpha-Chains; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000378:metabolism
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D006684:HLA-DR Antigens; D059805:HLA-DR alpha-Chains; D016209:Interleukin-8; D009838:Oligodeoxyribonucleotides; D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine; D004247:DNA