title: Enhancement of TREK1 channel surface expression by protein-protein interaction with beta-COP.
abstract: TREK1 belongs to a family of two-pore-domain K(+) (K(2P)) channels and produce background currents that regulate cell excitability. In the present study, we identified a vesicle transport protein, beta-COP, as an interacting partner by yeast two-hybrid screening of a human brain cDNA library with N-terminal region of TREK1 (TREK1-N) as bait. Several in vitro and in vivo binding assays confirmed the protein-protein interaction between TREK1 and beta-COP. We also found that beta-COP was associated with TREK1 in native condition at the PC3 cells. When RFP-beta-COP was co-transfected with GFP-TREK1 into COS-7 cells, both proteins were found localized to the plasma membrane. In addition, the channel activity and surface expression of GFP-TREK1 increased dramatically by co-transfection with RFP-beta-COP. Surface expression of the TREK1 channel was also clearly reduced with the addition of beta-COP-specific shRNA. Collectively, these data suggest that beta-COP plays a critical role in the forward transport of TREK1 channel to the plasma membrane.
authors.affiliation: Department of Physiology, Gyeongsang National University School of Medicine, Jinju 660-751, South Korea.
authors.forename: Eunju
authors.initials: E
authors.lastname: Kim
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Hwang
authors.forename: Oleg
authors.initials: O
authors.lastname: Yarishkin
authors.forename: Jae Cheal
authors.initials: JC
authors.lastname: Yoo
authors.forename: Donggyu
authors.initials: D
authors.lastname: Kim
authors.forename: Nammi
authors.initials: N
authors.lastname: Park
authors.forename: Minhee
authors.initials: M
authors.lastname: Cho
authors.forename: Young Sun
authors.initials: YS
authors.lastname: Lee
authors.forename: Choong-Hyun
authors.initials: CH
authors.lastname: Sun
authors.forename: Gwan-Su
authors.initials: GS
authors.lastname: Yi
authors.forename: Jiyun
authors.initials: J
authors.lastname: Yoo
authors.forename: Dawon
authors.initials: D
authors.lastname: Kang
authors.forename: Jaehee
authors.initials: J
authors.lastname: Han
authors.forename: Seong-Geun
authors.initials: SG
authors.lastname: Hong
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Park
mesh_terms: D000818:Animals; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism*; D002522:Chlorocebus aethiops; D020756:Coatomer Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D024683:Potassium Channels, Tandem Pore Domain / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D020798:Two-Hybrid System Techniques
chemical_list: D020756:Coatomer Protein; D024683:Potassium Channels, Tandem Pore Domain; C103742:potassium channel protein TREK-1