title: Inhibition of the osteoclast activity with the application of recombinant murine RANK protein.
abstract: OBJECTIVE
  To investigate the effects of recombinant Murine RANK on the osteoclast activity.
  
  
  METHODS
  Osteoclast was observed with soluble RANK. Female Wistar rats were bilaterally ovariectomized, and intra-abdominally injected with 5 mg/Kg soluble RANK. The bone metabolism, bone density, and bone histomorphology were observed.
  
  
  RESULTS
  Compared with the control group, the quantity of TRAP-positive osteoclasts and bone resorption pit counting in the rest groups were significantly reduced. The bone density of the dosed groups was significantly increased and TRAP-stained osteoclasts in bone tissue sections were almost inhibited.
  
  
  CONCLUSIONS
  rh-Murine RANK was able to inhibit osteoclast differentiation and prevent ovariectomy-induced bone loss.
authors.affiliation: Chinese PLA General Hospital, Department of Orthopedics, Beijing, PR China.
authors.forename: Peifu
authors.initials: P
authors.lastname: Tang
authors.forename: Licheng
authors.initials: L
authors.lastname: Zhang
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.forename: Lihai
authors.initials: L
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D001851:Bone Diseases, Metabolic / Q000188:drug therapy / Q000517:prevention & control; D001862:Bone Resorption / Q000097:blood / Q000188:drug therapy / Q000209:etiology / Q000503:physiopathology; D002118:Calcium / Q000097:blood; D002454:Cell Differentiation / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D051379:Mice; D015675:Osteocalcin / Q000097:blood; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010052:Ovariectomy / Q000009:adverse effects; D010758:Phosphorus / Q000097:blood; D051381:Rats; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D012995:Solubility
chemical_list: D053246:Receptor Activator of Nuclear Factor-kappa B; D011994:Recombinant Proteins; D015675:Osteocalcin; D010758:Phosphorus; D000469:Alkaline Phosphatase; D002118:Calcium