Predominance of an ST11 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae clone causing bacteraemia and urinary tract infections in Korea.
To investigate the antimicrobial resistance, extended-spectrum beta-lactamases (ESBLs) and clones of Klebsiella pneumoniae isolates causing bacteraemia or urinary tract infection (UTI) in Korea, a total of 406 K. pneumoniae isolates from patients with bacteraemia (221 isolates) and UTI (185 isolates) were collected from 10 tertiary-care Korean hospitals from July 2006 to October 2007. In vitro antimicrobial susceptibility testing was performed for all isolates and ESBL production was tested. Multilocus sequence typing (MLST) analyses were performed to characterize genotypes of ESBL-producing K. pneumoniae isolates. PFGE was performed for sequence type 11 (ST11) isolates. Forty-seven UTI isolates (25.4 %) produced ESBLs, while 30 bacteraemia isolates (13.6 %) produced ESBLs (P=0.002). Among 77 ESBL-producing isolates, thirty-two (41.6 %) produced SHV-type ESBLs. bla(CTX-M) genes such as bla(CTX-M-14) and bla(CTX-M-15) were detected in 36.4 %. MLST and PFGE analyses showed that ST11 was dominant in ESBL-producing K. pneumoniae isolates causing UTI (57.4 %) and in those causing bacteraemia (70.0 %) and has been prevalent in Korean hospitals. ST11 isolates harbour a combination of different ESBL genes. The ST11 clone of ESBL-producing K. pneumoniae isolates prevails in Korea, but most isolates might acquire ESBL genes independently or several different clones might be distributed in Korea.
Ko
Kwan Soo
KS
Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul 135-710, Republic of Korea.|Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
Lee
Ji-Young
JY
Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul 135-710, Republic of Korea.
Baek
Jin Yang
JY
Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul 135-710, Republic of Korea.
Suh
Ji-Yoeun
JY
Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul 135-710, Republic of Korea.
Lee
Mi Young
MY
Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul 135-710, Republic of Korea.
Choi
Ji Young
JY
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
Yeom
Joon-Sup
JS
Department of Internal Medicine, Kangbuk Samung Hospital, Sungkyunkwan University School of Medicine, Seoul 110-746, Republic of Korea.
Kim
Yeon-Sook
YS
Chungnam National University Hospital, Daejeon 301-721, Republic of Korea.
Jung
Sook-In
SI
Division of Infectious Diseases, Chonnam National University Medical School, Gwangju 501-757, Republic of Korea.
Shin
Sang Yop
SY
Jeju National University Hospital, Jeju National University School of Medicine, Jeju 690-716, Republic of Korea.
Heo
Sang Taek
ST
Department of Internal Medicine, Gyeongsang National University School of Medicine and Gyeongsang Institute of Health Sciences, Jinju 660-702, Republic of Korea.
Kwon
Ki Tae
KT
Daegu Fatima Hospital, Daegu 701-600, Republic of Korea.
Son
Jun Seong
JS
East-West Neo Medical Center, Kyunghee University, Seoul 134-727, Republic of Korea.
Kim
Shin-Woo
SW
Kyungpook National University Hospital, Daegu 700-721, Republic of Korea.
Chang
Hyun-Ha
HH
Kyungpook National University Hospital, Daegu 700-721, Republic of Korea.
Ki
Hyun Kyun
HK
Konkuk University Hospital, Seoul 143-729, Republic of Korea.
Chung
Doo Ryeon
DR
Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Republic of Korea.
Peck
Kyong Ran
KR
Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Republic of Korea.
Song
Jae-Hoon
JH
Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Republic of Korea.|Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul 135-710, Republic of Korea.
D016470:Bacteremia / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D007723:Korea / Q000453:epidemiology; D014552:Urinary Tract Infections / Q000453:epidemiology / Q000382:microbiology; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000378:metabolism*
D001618:beta-Lactamases