Accumulation of 1-deoxynojirimycin in silkworm, Bombyx mori L.
1-deoxynojirimycin (1-DNJ) contents in the silkworm, Bombyx mori, at different developmental stages and tissues were investigated by using reverse-phase high-performance liquid chromatography. The 1-DNJ contents of silkworm larvae change significantly with their developmental stages. The male larvae showed higher accumulation efficiency of 1-DNJ than the females and also a significant variation was observed among the silkworm strains. The present results show that tissue distribution of 1-DNJ was significantly higher in blood, digestive juice, and alimentary canal, but no 1-DNJ was observed in the silkgland. Moreover, 1-DNJ was not found in silkworms fed with artificial diet that does not contain mulberry leaf powder. This proves that silkworms obtain 1-DNJ from mulberry leaves; they could not synthesize 1-DNJ by themselves. The accumulation and excretion of 1-DNJ change periodically during the larval stage. There was no 1-DNJ in the newly-hatched larvae and 1-DNJ was mainly accumulated during the early and middle stages of every instar, while excreted at later stages of larval development. Further, it is possible to extract 1-DNJ from the larval feces and it is optimal to develop the 1-DNJ related products for diabetic auxiliary therapy.
Yin
Hao
H
Key Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University, Taian 271018, China.
Shi
Xin-qin
XQ
Sun
Bo
B
Ye
Jing-jing
JJ
Duan
Zu-an
ZA
Zhou
Xiao-ling
XL
Cui
Wei-zheng
WZ
Wu
Xiao-feng
XF
D017485:1-Deoxynojirimycin / Q000737:chemistry / Q000493:pharmacokinetics*; D000821:Animal Feed; D000818:Animals; D012831:Bombyx / Q000196:embryology / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D007814:Larva / Q000378:metabolism; D008297:Male; D031625:Morus; D018515:Plant Leaves / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution
D017485:1-Deoxynojirimycin