title: Interaction of annexin A6 with cholesterol rich membranes is pH-dependent and mediated by the sterol OH.
abstract: Annexin A6 (AnxA6), a calcium- and membrane-binding protein, is well-known to play a role in calcium homeostasis, membrane traffic and membrane organization. It had been suggested that, despite calcium-dependent interaction with anionic phospholipids, AnxA6 displays calcium-independent cholesterol binding properties. In this study, the following questions were addressed: does AnxA6 bind preferentially to cholesterol-containing biomimetic membranes? If so, what is the molecular mechanism of the binding? To answer these questions, human recombinant AnxA6-1 isoform was prepared and used with Langmuir monolayers containing various lipids. The interactions between AnxA6 and the lipid monolayers were examined by kinetic measurements of the interfacial adsorption and Brewster angle microscopy. We focused on the pH effect on the AnxA6 binding to monolayers containing cholesterol. At acidic pH, AnxA6-1 exhibits the highest affinity to monolayers containing the highest amount of cholesterol. Replacing cholesterol by cholesteryl acetate provided evidence that the hydroxyl group of cholesterol plays a role in AnxA6-lipid interactions. In addition, the affinity of recombinant AnxA6-1 tryptophan mutant (W343F) to the air/water interface and to lipid monolayers was tested. Substitution of Trp343 modified the interfacial properties of the protein and its interactions with sterol monolayers. Our results suggest that the linker region containing Trp343 is important for the interactions between AnxA6-1 and cholesterol.
authors.affiliation: Université de Lyon, Villeurbanne, F-69622, France.
authors.forename: Magdalena M
authors.initials: MM
authors.lastname: Domon
authors.forename: Gladys
authors.initials: G
authors.lastname: Matar
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Strzelecka-Kiliszek
authors.forename: Joanna
authors.initials: J
authors.lastname: Bandorowicz-Pikula
authors.forename: Slawomir
authors.initials: S
authors.lastname: Pikula
authors.forename: Françoise
authors.initials: F
authors.lastname: Besson
mesh_terms: D017317:Annexin A6 / Q000235:genetics / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D002788:Cholesterol Esters / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008563:Membrane Lipids / Q000737:chemistry*; D009154:Mutation; D010743:Phospholipids / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013261:Sterols / Q000378:metabolism
chemical_list: D017317:Annexin A6; D002788:Cholesterol Esters; D008563:Membrane Lipids; D010743:Phospholipids; D020033:Protein Isoforms; D011994:Recombinant Proteins; D013261:Sterols; D002784:Cholesterol; C060123:cholesterol acetate