title: Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.
abstract: Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the approximately 21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes. Phenotypes were scored quantitatively by computational image processing, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival. As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community.
authors.affiliation: MitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
authors.forename: Beate
authors.initials: B
authors.lastname: Neumann
authors.forename: Thomas
authors.initials: T
authors.lastname: Walter
authors.forename: Jean-Karim
authors.initials: JK
authors.lastname: Hériché
authors.forename: Jutta
authors.initials: J
authors.lastname: Bulkescher
authors.forename: Holger
authors.initials: H
authors.lastname: Erfle
authors.forename: Christian
authors.initials: C
authors.lastname: Conrad
authors.forename: Phill
authors.initials: P
authors.lastname: Rogers
authors.forename: Ina
authors.initials: I
authors.lastname: Poser
authors.forename: Michael
authors.initials: M
authors.lastname: Held
authors.forename: Urban
authors.initials: U
authors.lastname: Liebel
authors.forename: Cihan
authors.initials: C
authors.lastname: Cetin
authors.forename: Frank
authors.initials: F
authors.lastname: Sieckmann
authors.forename: Gregoire
authors.initials: G
authors.lastname: Pau
authors.forename: Rolf
authors.initials: R
authors.lastname: Kabbe
authors.forename: Annelie
authors.initials: A
authors.lastname: Wünsche
authors.forename: Venkata
authors.initials: V
authors.lastname: Satagopam
authors.forename: Michael H A
authors.initials: MH
authors.lastname: Schmitz
authors.forename: Catherine
authors.initials: C
authors.lastname: Chapuis
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Gerlich
authors.forename: Reinhard
authors.initials: R
authors.lastname: Schneider
authors.forename: Roland
authors.initials: R
authors.lastname: Eils
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Huber
authors.forename: Jan-Michael
authors.initials: JM
authors.lastname: Peters
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Hyman
authors.forename: Richard
authors.initials: R
authors.lastname: Durbin
authors.forename: Rainer
authors.initials: R
authors.lastname: Pepperkok
authors.forename: Jan
authors.initials: J
authors.lastname: Ellenberg
mesh_terms: D000818:Animals; D002455:Cell Division / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D003116:Color; D055785:Gene Knockdown Techniques; D005796:Genes / Q000235:genetics; D015894:Genome, Human / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D008938:Mitosis / Q000235:genetics; D010641:Phenotype; D034622:RNA Interference; D015203:Reproducibility of Results; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism; D013997:Time Factors