title: Synthesis and characterization of a fluorescent water-soluble paclitaxel prodrug.
abstract: A fluorescence susceptible water-soluble paclitaxel was synthesized by a condensation reaction between PEGylated paclitaxel (namely, PP7) and 1-pyrene butyric acid (PBA) in order to obtain a better understanding of the mechanism of action of paclitaxel as well as of the environment of the paclitaxel-binding site. The reaction was performed successfully and the resulting paclitaxel was characterized by FT-NMR, analytical-HPLC, UV spectro photometry, and fluorescence spectrometry. The synthesized paclitaxel analogue showed a high susceptibility to fluorescence in both excitation and emission spectra. And we have investigated the time-resolved fluorescence behavior of them in different solvents and at different excitation wavelengths.
authors.affiliation: Department of Polymer Science and Engineering, Chosun University, Gwangju 501-759, Korea.
authors.forename: Jeong-Sun
authors.initials: JS
authors.lastname: Sohn
authors.forename: Eun-Sun
authors.initials: ES
authors.lastname: Choi
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Jo
authors.forename: Michael
authors.initials: M
authors.lastname: Hess
authors.forename: Song-Hee
authors.initials: SH
authors.lastname: Han
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D017239:Paclitaxel / Q000737:chemistry*; D011355:Prodrugs / Q000737:chemistry*; D012995:Solubility; D013194:Staining and Labeling / Q000379:methods; D014867:Water / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005456:Fluorescent Dyes; D011355:Prodrugs; D014867:Water; D017239:Paclitaxel