Inhibition of antigen-induced degranulation by aryl compounds isolated from the bark of Betula platyphylla in RBL-2H3 cells.
The methanolic extract of the bark of Betula platyphylla was found to suppress antigen mediated degranulation of RBL-2H3 cells. Four arylbutanoids (1-4) and eight diarylhepatonoids (5-12) were isolated from the methanolic extract using bioassay-guided fractionation. Among them, compounds 4 and 12 were isolated and assigned for the first time. Compounds 1, 2, 3, 5, 10, and 12 showed remarkable inhibitory activity against the degranulation of RBL-2H3 by antigen stimulation in a dose dependent manner at the concentrations ranging from 10 microM to 100 microM.
Kim
Seung Hyun
SH
Institute for Life Science, Elcomscience Co. Ltd., San 56-1, Sillim-Dong, Gwanak-Gu, Seoul 151-742, Republic of Korea.
Park
Jung Hyun
JH
Kim
Tae Bum
TB
Lee
Hyang Hwa
HH
Lee
Ki Yong
KY
Kim
Young Choong
YC
Sung
Sang Hyun
SH
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000941:Antigens / Q000276:immunology; D029662:Betula / Q000737:chemistry*; D015550:Cell Degranulation / Q000187:drug effects*; D045744:Cell Line, Tumor; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D015093:Meglutol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D024301:Plant Bark / Q000737:chemistry; D051381:Rats; D014312:Trisaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D000941:Antigens; D005960:Glucosides; D010636:Phenols; D014312:Trisaccharides; D015093:Meglutol; D001619:beta-N-Acetylhexosaminidases