Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects.
The medical significance of circulating endothelial or hematopoietic progenitors is becoming increasing recognized. While therapeutic augmentation of circulating progenitor cells using G-CSF has resulted in promising preclinical and early clinical data for several degenerative conditions, this approach is limited by cost and inability to perform chronic administration. Stem-Kine is a food supplement that was previously reported to augment circulating EPC in a pilot study. Here we report a trial in 18 healthy volunteers administered Stem-Kine twice daily for a 2 week period. Significant increases in circulating CD133 and CD34 cells were observed at days 1, 2, 7, and 14 subsequent to initiation of administration, which correlated with increased hematopoietic progenitors as detected by the HALO assay. Augmentation of EPC numbers in circulation was detected by KDR-1/CD34 staining and colony forming assays. These data suggest Stem-Kine supplementation may be useful as a stimulator of reparative processes associated with mobilization of hematopoietic and endothelial progenitors.
Mikirova
Nina A
NA
Medistem Inc, San Diego, California, USA.
Jackson
James A
JA
Hunninghake
Ron
R
Kenyon
Julian
J
Chan
Kyle W H
KW
Swindlehurst
Cathy A
CA
Minev
Boris
B
Patel
Amit N
AN
Murphy
Michael P
MP
Smith
Leonard
L
Ramos
Famela
F
Ichim
Thomas E
TE
Riordan
Neil H
NH
D000071916:AC133 Antigen; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D001681:Biological Assay; D002452:Cell Count; D002465:Cell Movement; D003114:Colony-Forming Units Assay; D019587:Dietary Supplements; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism; D010641:Phenotype; D055815:Young Adult
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides