title: Evolutionary relationships in the Drosophila ananassae species cluster based on introns of multiple nuclear loci.
abstract: The Drosophila ananassae species cluster includes D. ananassae, D. pallidosa, D. parapallidosa, and the cryptic species "pallidosa-like", "pallidosa-like Wau" and "papuensis-like" Some of the taxa are sympatric in the South Pacific, Papua New Guinea, and Southeast Asia, and gene flow between different taxa has been suspected for a handful of genes. In the present analysis, we examined DNA sequences of introns in four loci: alpha actinin (Actn) on XL, white (w) on XR, CG7785 on 2L, and zinc ion transmembrane transporter 63C (ZnT63C) on 2R. Phylogenetic trees (neighbor-joining and haplotype network) were inconsistent among these loci. Some haplotypes shared between taxa were found for w, CG7785, and ZnT63C, suggesting recent gene flow. However, no haplotypes were shared, for example, between D. ananassae and D. pallidosa for CG7785, which is close to the proximal breakpoint of In(2L)D. This suggests that taxon-specific inversions prevent gene flow, as predicted by the chromosomal speciation hypothesis.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan. sawamura@biol.tsukuba.ac.jp
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Sawamura
authors.forename: Koichi
authors.initials: K
authors.lastname: Kamiya
authors.forename: Hajime
authors.initials: H
authors.lastname: Sato
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Tomimura
authors.forename: Muneo
authors.initials: M
authors.lastname: Matsuda
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Oguma
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004330:Drosophila / Q000235:genetics*; D049810:Genetic Speciation; D007438:Introns / Q000235:genetics*; D010802:Phylogeny; D057345:Sequence Inversion
chemical_list: D004247:DNA