title: Comparative metabolomics charts the impact of genotype-dependent methionine accumulation in Arabidopsis thaliana.
abstract: Methionine (Met) is an essential amino acid for all organisms. In plants, Met also functions as a precursor of plant hormones, polyamines, and defense metabolites. The regulatory mechanism of Met biosynthesis is highly complex and, despite its great importance, remains unclear. To investigate how accumulation of Met influences metabolism as a whole in Arabidopsis, three methionine over-accumulation (mto) mutants were examined using a gas chromatography-mass spectrometry-based metabolomics approach. Multivariate statistical analyses of the three mto mutants (mto1, mto2, and mto3) revealed distinct metabolomic phenotypes. Orthogonal projection to latent structures-discriminant analysis highlighted discriminative metabolites contributing to the separation of each mutant and the corresponding control samples. Though Met accumulation in mto1 had no dramatic effect on other metabolic pathways except for the aspartate family, metabolite profiles of mto2 and mto3 indicated that several extensive pathways were affected in addition to over-accumulation of Met. The pronounced changes in metabolic pathways in both mto2 and mto3 were associated with polyamines. The findings suggest that our metabolomics approach not only can reveal the impact of Met over-accumulation on metabolism, but also may provide clues to identify crucial pathways for regulation of metabolism in plants.
authors.affiliation: RIKEN Plant Science Center, Yokohama, Kanagawa, Japan.
authors.forename: Miyako
authors.initials: M
authors.lastname: Kusano
authors.forename: Atsushi
authors.initials: A
authors.lastname: Fukushima
authors.forename: Henning
authors.initials: H
authors.lastname: Redestig
authors.forename: Makoto
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Hitomi
authors.initials: H
authors.lastname: Otsuki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Onouchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Naito
authors.forename: Masami Yokota
authors.initials: MY
authors.lastname: Hirai
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D053858:Metabolic Networks and Pathways; D055432:Metabolomics; D008715:Methionine / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D030821:Plants, Genetically Modified
chemical_list: D000596:Amino Acids; D029681:Arabidopsis Proteins; D018744:DNA, Plant; D008715:Methionine