[Effect and mechanism of ropivacaine on the isolated human umbilical artery].
OBJECTIVE
To investigation the effect of ropivacaine on the contraction of the isolated human umbilical artery and the mechanisms involved.


METHODS
Endothelium-denuded human umbilical artery rings obtained from healthy full-term parturients were prepared. Using isometric force transducers and a fluorometer, the effect of ropivacaine in cumulative concentration on the contraction response induced by KCl in the presence or absence of verapamil, or verapamil plus ruthenium red or verapamil plus heparin was observed. Furthermore, the effect of ropivacaine on the contraction response of the artery rings incubated in different concentrations of extracellular Ca(2+) was also observed.


RESULTS
Ropivacaine induced a dose-dependent biphasic contractile response of human umbilical artery rings: increasing at concentrations of 1.0 x 10(-5) to 1.0 x 10(-4) mol/L and decreasing from 3.0 x 10(-4) to 3.0 x 10(-3) mol/L, which was inhibited by verapamil, or verapamil plus ruthenium red, or verapamil plus heparin. No difference was found between pre-treatment of verapamil, verapamil plus ruthenium red and verapamil plus heparin. Ropivacaine induced no contractile response in Ca(2+)-free solution and a extracellular Ca(2+) dose-dependent increasing contractile response (1.0 x 10(-4) to 3.0 x 10(-2) mol/L).


CONCLUSIONS
Ropivacaine induced a dose-dependent biphasic contractile response of human umbilical artery rings. The increase in intracellular Ca(2+) concentrations by the extracellular Ca(2+) influx, not by the release from the sarcoplasmic reticulum, is involved in ropivacaine-induced vasoconstriction of human umbilical artery smooth muscle.
Feng
Ying
Y
Department of Anesthesia, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
Fei
Jia-qian
JQ
Qian
Xiao-wei
XW
Zhou
Hong-bin
HB
Xu
Gu-feng
GF
Wang
Lin-lin
LL
Lu
Hui-shun
HS
Chen
Xin-zhong
XZ
D000577:Amides / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D000077212:Ropivacaine; D014469:Umbilical Arteries / Q000187:drug effects* / Q000378:metabolism; D014661:Vasoconstriction / Q000187:drug effects*
D000577:Amides; D000077212:Ropivacaine; D002118:Calcium