Detection of Hb Constant Spring by a capillary electrophoresis method.
Hb Constant Spring [Hb CS; alpha142, Term-->Gln (TAA>CAA in alpha2)] is the most prevalent nondeletional alpha-thalassemia (alpha-thal) anomaly in southern China. In conjunction with alpha(0)-thal, it can cause severe Hb H (beta(4)) disease. The present study was done to evaluate the efficiency of two diagnostic methods in detecting Hb CS. Automated high performance liquid chromatography (HPLC) and Sebia Capillarys 2, a capillary electrophoresis method, were applied to blood samples from 21 individuals with Hb CS trait. Of the 21 cases, all (100%) were detected by capillary electrophoresis, whereas only 16 (76.2%) were detected by HPLC. We concluded that the Sebia Capillarys 2 is the preferred method for Hb CS screening.
Liao
Can
C
Prenatal Diagnostic Center, Guangzhou Maternal & Neonatal Hospital, Guangzhou Women & Children Medical Center Guangzhou Medical College, Guangzhou, Guangdong, People's Republic of China.
Zhou
Jian-Ying
JY
Xie
Xing-Mei
XM
Li
Jian
J
Li
Ru
R
Li
Dong-Zhi
DZ
D000328:Adult; D001797:Blood Protein Electrophoresis / Q000379:methods*; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D008297:Male; D008403:Mass Screening / Q000379:methods; D017085:alpha-Thalassemia / Q000097:blood* / Q000175:diagnosis
D006455:Hemoglobins, Abnormal; C035541:Hemoglobin Constant Spring