title: 2-DE proteomic approach to the Botrytis cinerea secretome induced with different carbon sources and plant-based elicitors.
abstract: Botrytis cinerea is a phytopathogenic fungus infecting a number of crops (tomatoes, grapes and strawberries), which has been adopted as a model system in molecular phytopathology. B. cinerea uses a wide variety of infection strategies, which are mediated by a set of genes/proteins called pathogenicity/virulence factors. Many of these factors have been described as secreted proteins, and thus the study of this sub-proteome, the secretome, under changing circumstances can help us to understand the roles of these factors, possibly revealing new loci for the fight against the pathogen. A 2-DE, MALDI TOF/TOF-based approach has been developed to establish the proteins secreted to culture media supplemented with different carbon sources and plant-based elicitors (in this study: glucose, cellulose, starch, pectin and tomato cell walls). Secreted proteins were obtained from the culture media by deoxycholate-trichloroacetic acid/phenol extraction, and 76 spots were identified, yielding 95 positive hits that correspond to 56 unique proteins, including several known virulence factors (i.e. pectin methyl esterases, xylanases and proteases). The observed increases in secretion of proteins with established virulence-related functions indicate that this in vitro-induction/proteome-mining approach is a promising strategy for discovering new pathogenicity factors and dissecting infection mechanisms in a discrete fashion.
authors.affiliation: Marine and Environmental Sciences Faculty, University of Cádiz, Puerto Real, Cádiz, Spain. franciscojavier.fernandez@uca.es
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Fernández-Acero
authors.forename: Thomas
authors.initials: T
authors.lastname: Colby
authors.forename: Anne
authors.initials: A
authors.lastname: Harzen
authors.forename: Maria
authors.initials: M
authors.lastname: Carbú
authors.forename: Ursula
authors.initials: U
authors.lastname: Wieneke
authors.forename: Jesús Manuel
authors.initials: JM
authors.lastname: Cantoral
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schmidt
mesh_terms: D020171:Botrytis / Q000187:drug effects* / Q000378:metabolism*; D002244:Carbon / Q000494:pharmacology*; D002482:Cellulose / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D010368:Pectins / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D010944:Plants / Q000737:chemistry*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013213:Starch / Q000494:pharmacology
chemical_list: D010936:Plant Extracts; D002244:Carbon; D010368:Pectins; D002482:Cellulose; D013213:Starch; D005947:Glucose