title: Improvement of coenzyme Q(10) production by increasing the NADH/NAD(+) ratio in Agrobacterium tumefaciens.
abstract: Previously screened CoQ(10)-overproducing Agrobacterium tumefaciens A603-35 showed a relatively high NADH/NAD(+) ratio (1.1), as compared to parental strain C58 (0.2) when we increased the expression levels of NADH-generating enzymes. Also, the intracellular NADH/NAD(+) ratio showed a positive correlation with the CoQ(10) content in A603-35. Overexpression of glyceraldehyde 3-phosphate dehydrogenase in A603-35 shifted the NADH/NAD(+) ratio at 48 h from 0.8 to 1.2, and thus the CoQ(10) content in flask culture increased from 2.16 to 3.63 mg/g DCW. Due to the addition of hydroxybutyrate to the culture media, the intracellular NADH/NAD(+) ratio in A603-35-gapA shifted from 1.2 to 1.4, which led to an increase CoQ(10) content (5.27 mg/g DCW).
authors.affiliation: BioNgene Co., Ltd., Seoul, Republic of Korea.
authors.forename: Bong-Seong
authors.initials: BS
authors.lastname: Koo
authors.forename: Yun-Jung
authors.initials: YJ
authors.lastname: Gong
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Kim
authors.forename: Chan-Wha
authors.initials: CW
authors.lastname: Kim
authors.forename: Hyeon-Cheol
authors.initials: HC
authors.lastname: Lee
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005986:Glyceraldehyde 3-Phosphate / Q000235:genetics / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D009243:NAD / Q000235:genetics / Q000378:metabolism*; D014451:Ubiquinone / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D003470:Culture Media; D009243:NAD; D014451:Ubiquinone; D005986:Glyceraldehyde 3-Phosphate; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases