title: Auto-transplanted mesenchymal stromal cell fate in periodontal tissue of beagle dogs.
abstract: OBJECTIVE
  Mesenchymal stromal cells (MSC) possess multilineage differentiation potential and characteristics of self-renewal. It has been reported that MSC can acquire characteristics of cells in the periodontal ligament (PDL) in vitro. Moreover, the transplantation of MSC has been shown to be a promising strategy for treating periodontal defects. However, little is known about the fate of MSC in periodontal tissue in vivo. The aim of this study was to trace the paths of MSC after transplantation into periodontal tissues in vivo.
  
  
  METHODS
  MSC labeled with bromodeoxyuridine (BrdU) were transplanted into periodontal defects of beagle dogs. Six weeks after surgery, the animals were killed and decalcified specimens were prepared. Migration and differentiation of MSC were detected by single/double immunohistochemistry and a combination of immunohistochemistry and in situ hybridization.
  
  
  RESULTS
  BrdU-labeled MSC were observed distributing into periodontal tissue that included alveolar bone, PDL, cementum and blood vessels and expressing surface markers typical of osteoblasts and fibroblasts.
  
  
  CONCLUSIONS
  Cumulatively, our data suggest that MSC migrate throughout periodontal tissue and differentiate into osteoblasts and fibroblasts after transplantation into periodontal defects at 6 weeks in vivo, and have the potential to regenerate periodontal tissue.
authors.affiliation: State Key Laboratory of Oral Disease, Sichuan University, Chengdu, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Wei
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.forename: Dapeng
authors.initials: D
authors.lastname: Liao
authors.forename: Xingmei
authors.initials: X
authors.lastname: Yang
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.forename: Yurong
authors.initials: Y
authors.lastname: Liu
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
authors.forename: Zhen
authors.initials: Z
authors.lastname: Tan
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D004285:Dogs; D017823:Furcation Defects / Q000473:pathology / Q000628:therapy*; D016557:Guided Tissue Regeneration, Periodontal; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D023421:Models, Animal; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010513:Periodontal Ligament / Q000166:cytology / Q000502:physiology*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D014182:Transplantation, Autologous
chemical_list: D015415:Biomarkers; D001973:Bromodeoxyuridine