Colloidal gold probe-based immunochromatographic assay for the rapid detection of lead ions in water samples.
One-step immunochromatographic assay (ICA) has been developed using colloidal gold-labeled monoclonal antibody probe for the rapid detection of lead ions in water samples. The ICA was based on the theory of competitive reactivity, and the results can be easily judged based on the presence or absence of a red colored test line with visual detection. Under optimal conditions, this method shows high detecting sensitivity with a LOD (limit of detection) of 50 ng/ml. Stability test indicates that the immunochromatographic strips are stable for 8 weeks at room temperature. During practical application, nanometer TiO2 is used to enrich the lead ions in water samples. The ICA is successfully applied in the measurement of lead ion concentrations in local water samples, and the results are highly consistent with that of ICP-MS. Detecting lead ions with ICA can be done within 4 min and is very useful for the rapid onsite testing.
Tang
Yong
Y
Center of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China.
Zhai
Yi-Fan
YF
Xiang
Jun-Jian
JJ
Wang
Hong
H
Liu
Bin
B
Guo
Chang-Wei
CW
D000911:Antibodies, Monoclonal / Q000737:chemistry; D000941:Antigens / Q000737:chemistry; D002853:Chromatography, Liquid; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D017979:Gold Colloid / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007477:Ions; D007854:Lead / Q000032:analysis*; D013058:Mass Spectrometry; D046529:Microscopy, Electron, Transmission; D010047:Ovalbumin / Q000737:chemistry; D004369:Pentetic Acid / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000941:Antigens; D017979:Gold Colloid; D007477:Ions; D014874:Water Pollutants, Chemical; D007854:Lead; D004369:Pentetic Acid; D010047:Ovalbumin