Increasing anthraquinone production by overexpression of 1-deoxy-D: -xylulose-5-phosphate synthase in transgenic cell suspension cultures of Morinda citrifolia.
A Morinda citrifolia cell line was obtained by overexpresion of 1-deoxy-D: -xylulose 5-phosphate synthase (DXS) from Catharanthus roseus, a key enzyme of the metabolic pathway of anthraquinones (AQs). This cell line increased AQs production by about 24% compared to the control cell line. This transgenic cell line which carries dxs cDNA isolated from Catharanthus roseus, was achieved by direct transformation of cell suspension cultures of M. citrifolia using a hypervirulent Agrobacterium tumefaciens strain. The effects of the overexpression of the dxs gene also resulted in increased levels of dxs mRNA transcripts and DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonia-lyase activity were evaluated and were significantly higher in the transgenic line than in controls.
Quevedo
Carla
C
Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113, Cuidad Autónoma de Buenos Aires, Argentina.
Perassolo
María
M
Alechine
Eugenia
E
Corach
Daniel
D
Giulietti
Ana María
AM
Talou
Julián Rodriguez
JR
D016960:Agrobacterium tumefaciens / Q000235:genetics; D000880:Anthraquinones / Q000378:metabolism*; D030008:Catharanthus / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D005822:Genetic Vectors; D032066:Morinda / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010636:Phenols / Q000032:analysis; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D014166:Transferases / Q000235:genetics / Q000378:metabolism*
D000880:Anthraquinones; D010636:Phenols; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D014166:Transferases; C109461:deoxyxylulose-5-phosphate synthase; D010650:Phenylalanine Ammonia-Lyase