title: The profile of mitochondrial proteins and their phosphorylation signaling network in INS-1 beta cells.
abstract: Mitochondria have important roles in cellular physiological functions and various diseases. In pancreatic beta cells, mitochondria play a central role in glucose-stimulated insulin secretion (GSIS). To reveal the potential functions of mitochondria in the GSIS process in beta cells, shotgun proteomics was applied to profiling mitochondrial proteins and their potential phosphorylation sites in rat INS-1 cells. More than 800 proteins were assigned to mitochondria. In addition, 84 different mitochondrial phosphoproteins were identified, and 52 upstream kinases of mitochondrial phosphoproteins were predicted using bioinformatics tools. Regulation networks of mitochondrial phosphoproteins were constructed by integrating mitochondrial protein interaction networks and mitochondrial phosphorylation signaling, providing a preliminary survey of how phosphorylation signaling regulates mitochondrial function in beta cells. We present integrated resources including the protein composition and signaling pathways of mitochondria which can be used to understand the role of mitochondria in GSIS.
authors.affiliation: Laborotary of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Ziyou
authors.initials: Z
authors.lastname: Cui
authors.forename: Junjie
authors.initials: J
authors.lastname: Hou
authors.forename: Xiulan
authors.initials: X
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Xie
authors.forename: Peng
authors.initials: P
authors.lastname: Xue
authors.forename: Tanxi
authors.initials: T
authors.lastname: Cai
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Fuquan
authors.initials: F
authors.lastname: Yang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D019295:Computational Biology / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000737:chemistry / Q000378:metabolism*; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000378:metabolism*; D010748:Phosphopeptides / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D025941:Protein Interaction Mapping; D011494:Protein Kinases / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D015203:Reproducibility of Results; D015398:Signal Transduction; D012984:Software; D053719:Tandem Mass Spectrometry
chemical_list: D005227:Fatty Acids; D024101:Mitochondrial Proteins; D010748:Phosphopeptides; D010750:Phosphoproteins; D020543:Proteome; D011494:Protein Kinases