title: Serum amyloid P inhibits fibrosis through Fc gamma R-dependent monocyte-macrophage regulation in vivo.
abstract: New therapies that target chronic inflammation with fibrosis are urgently required. Increasing evidence points to innate activation of inflammatory cells in driving chronic organ fibrosis. Serum amyloid P is a naturally circulating soluble pattern recognition receptor, a member of the family of pentraxin proteins. It links danger-associated molecular pattern recognition to Fc gamma receptor-mediated phagocytosis. Here we show that fibrosis progression in the mouse kidney is significantly inhibited by therapeutic administration of human serum amyloid P, regulated by activating Fc gamma receptors, and dependent on inflammatory monocytes and macrophages, but not fibrocytes. Human serum amyloid P-mediated inhibition of mouse kidney fibrosis correlated with specific binding of human serum amyloid P to cell debris and with subsequent suppression of inflammatory monocytes and kidney macrophages in vitro and in vivo, and was dependent on regulated binding to activating Fc gamma receptors and interleukin-10 expression. These studies uncover previously unidentified roles for Fc gamma receptors in sterile inflammation and highlight serum amyloid P as a potential antifibrotic therapy through local generation of interleukin-10.
authors.affiliation: Laboratory of Inflammation Research, Harvard Institutes of Medicine, 4 Blackfan Circle, Boston, MA 02115, USA.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Castaño
authors.forename: Shuei-Liong
authors.initials: SL
authors.lastname: Lin
authors.forename: Teresa
authors.initials: T
authors.lastname: Surowy
authors.forename: Brian T
authors.initials: BT
authors.lastname: Nowlin
authors.forename: Swathi A
authors.initials: SA
authors.lastname: Turlapati
authors.forename: Tejas
authors.initials: T
authors.lastname: Patel
authors.forename: Ajay
authors.initials: A
authors.lastname: Singh
authors.forename: Shawn
authors.initials: S
authors.lastname: Li
authors.forename: Mark L
authors.initials: ML
authors.lastname: Lupher
authors.forename: Jeremy S
authors.initials: JS
authors.lastname: Duffield
mesh_terms: D000818:Animals; D005355:Fibrosis / Q000517:prevention & control*; D006801:Humans; D008264:Macrophages / Q000737:chemistry* / Q000166:cytology*; D051379:Mice; D009000:Monocytes / Q000166:cytology*; D017452:Receptors, IgG / Q000502:physiology*; D000683:Serum Amyloid P-Component / Q000502:physiology*
chemical_list: D017452:Receptors, IgG; D000683:Serum Amyloid P-Component