Pilot-scale production of microbial lipid using starch wastewater as raw material.
The aim of this research was to develop a cost-effective pilot-scale fermentation process for lipid production by Rhodotorula glutinis using starch wastewater. A temperature range of 30-37 degrees C, and an initial chemical oxygen demand (COD) higher than 30,000 mg/L, were optimal for growth and lipid synthesis in flask culture. Cultivation in a 5-L fermenter yielded more than 60 g/L biomass with a 30% (w/w) lipid content after 60 h. Pilot-scale production of microbial lipid in a 300-L fermenter using starch wastewater without sterilization and pH adjustment yielded 40 g/L biomass and 35% lipid content with 80% COD degradation after only 30-40 h of cultivation. Transesterification experiments demonstrated that lipids can be used for biodiesel production.
Xue
Feiyan
F
Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Gao
Bin
B
Zhu
Yongqiang
Y
Zhang
Xu
X
Feng
Wei
W
Tan
Tianwei
T
D019149:Bioreactors / Q000382:microbiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D050356:Lipid Metabolism / Q000502:physiology*; D010865:Pilot Projects; D012248:Rhodotorula / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
D014874:Water Pollutants, Chemical; D013213:Starch