title: [Complete genomic sequence of rapidly replicating strain MB-7 of hepatitis a virus and its characterization in comparison with nucleotide sequences of other hepatitis A virus strains].
abstract: Complete nucleotide sequence of genomic RNA of hepatitis A virus (HAV) rapidly replicating strain MB-7 was determined. Comparison of nucleotide and deduced amino-acid sequences demonstrated the highest level of identity of MB-7 with strain HAS-15 (above 99%) and high homology with other HAV strains (HM 175/7, CR326, and GBM/HFS) used in production of anti-hepatitis A vaccines. MB-7 was classified as subgenotype IA. Phylogenetic analysis showed that MB-7 is most closely related to the strain HAS-15 and the HAV variants circulating in Russia. Comparative analysis of genomic differences between MB-7 and HAS-15 with other HAV strains revealed among changes characteristic of MB-7 those typical of the described earlier rapidly replicating HAV strains (nt. 149-162 in 5'-untranslated region and changes in the VP3 and 2C genes). These results suggest the functional importance of changes in above-mentioned regions of HAV genome for the increased replication level of MB-7 in vitro.
authors.forename: T Iu
authors.initials: TIu
authors.lastname: Bondarenko
authors.forename: V A
authors.initials: VA
authors.lastname: Ternovoĭ
authors.forename: V A
authors.initials: VA
authors.lastname: Sviatchenko
authors.forename: N N
authors.initials: NN
authors.lastname: Kiselev
authors.forename: E V
authors.initials: EV
authors.lastname: Chausov
authors.forename: M A
authors.initials: MA
authors.lastname: Muntianova
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Nemtsov
authors.forename: V V
authors.initials: VV
authors.lastname: Iashin
authors.forename: N I
authors.initials: NI
authors.lastname: Kriuk
authors.forename: A G
authors.initials: AG
authors.lastname: Kusliĭ
authors.forename: L G
authors.initials: LG
authors.lastname: Nikulin
authors.forename: S V
authors.initials: SV
authors.lastname: Netesov
mesh_terms: D001483:Base Sequence; D006506:Hepatitis A / Q000821:virology*; D017957:Hepatitis A Virus, Human / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017423:Sequence Analysis, RNA; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins