Conjugation and radiolabeling of monoclonal antibodies with zirconium-89 for PET imaging using the bifunctional chelate p-isothiocyanatobenzyl-desferrioxamine.
The positron emitter zirconium-89 ((89)Zr) has very attractive properties for positron emission tomography (PET) imaging of intact monoclonal antibodies (mAbs) using immuno-PET. This protocol describes the step-by-step procedure for the facile radiolabeling of mAbs or other proteins with (89)Zr using p-isothiocyanatobenzyl-desferrioxamine (Df-Bz-NCS). First, Df-Bz-NCS is coupled to the lysine-NH(2) groups of a mAb at pH 9.0 (pre-modification), followed by purification using gel filtration. Next, the pre-modified mAb is labeled at room temperature by the addition of [(89)Zr]Zr-oxalic acid solution followed by purification using gel filtration. The entire process of pre-modification, radiolabeling and purification steps will take about 2.5 h.
Vosjan
Maria J W D
MJ
Department of Otolaryngology, VU University Medical Center, Amsterdam, The Netherlands.
Perk
Lars R
LR
Visser
Gerard W M
GW
Budde
Marianne
M
Jurek
Paul
P
Kiefer
Garry E
GE
van Dongen
Guus A M S
GA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002614:Chelating Agents; D003676:Deferoxamine / Q000031:analogs & derivatives; D006258:Head and Neck Neoplasms / Q000000981:diagnostic imaging; D006801:Humans; D017879:Isothiocyanates; D051379:Mice; D008819:Mice, Nude; D049268:Positron-Emission Tomography / Q000379:methods*; D011868:Radioisotopes; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000737:chemistry; D014183:Transplantation, Heterologous; D015040:Zirconium
C549125:4-isothiocyanatobenzyl-desferrioxamine; D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D017879:Isothiocyanates; D011868:Radioisotopes; D019275:Radiopharmaceuticals; D015040:Zirconium; D003676:Deferoxamine