Involvement of the mannose phosphotransferase system of Lactobacillus plantarum WCFS1 in peroxide stress tolerance.
A Lactobacillus plantarum strain with a deletion in the gene rpoN, encoding the alternative sigma factor 54 (sigma(54)), displayed a 100-fold-higher sensitivity to peroxide than its parental strain. This feature could be due to sigma(54)-dependent regulation of genes involved in the peroxide stress response. However, transcriptome analyses of the wild type and the mutant strain during peroxide exposure did not support such a role for sigma(54). Subsequent experiments revealed that the impaired expression of the mannose phosphotransferase system (PTS) operon in the rpoN mutant caused the observed increased peroxide sensitivity.
Stevens
Marc J A
MJ
TI Food and Nutrition, Wageningen, Netherlands.
Molenaar
Douwe
D
de Jong
Anne
A
de Vos
Willem M
WM
Kleerebezem
Michiel
M
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000201:enzymology*; D008969:Molecular Sequence Data; D009876:Operon; D018384:Oxidative Stress; D010545:Peroxides / Q000633:toxicity*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism; D051877:RNA Polymerase Sigma 54 / Q000172:deficiency; D013312:Stress, Physiological
D001426:Bacterial Proteins; D010545:Peroxides; D017853:Phosphotransferases (Alcohol Group Acceptor); C032922:mannokinase; D051877:RNA Polymerase Sigma 54