title: The role of T-lymphocyte subsets and interleukin-5 blood levels among Indian subjects with autoimmune thyroid disease.
abstract: OBJECTIVE
  Autoimmune Thyroid Disease (AITD) results from an interaction of exogenous and endogenous factors in a genetically predisposed individual. AITD is being increasingly reported among the Indian population. Lymphocyte subsets and levels of interleukin-5 (IL- 5) were studied in the peripheral blood of patients with AITD.
  
  
  METHODS
  Subjects diagnosed with either hyperthyroidism due to Graves' Disease (GD) or with primary hypothyroidism due to Hashimoto's Thyroiditis (HT) were consecutively recruited. Euthyroid controls were also recruited for comparison. Lymphocyte subsets (CD4 and CD8 counts, CD4/CD8 ratio) were evaluated by flow cytometry and IL-5 levels were determined by the sandwich ELISA method.
  
  
  RESULTS
  Nineteen subjects with GD, 16 subjects with HT and 10 controls were studied. CD4/CD8 ratios were found to be significantly lower only in subjects with HT compared to controls. Serum IL-5 values were significantly higher in both GD and HT in comparison to controls.
  
  
  CONCLUSIONS
  The study found increased levels of IL-5 and reduction in ratios of CD4/CD8 lymphocytes in the peripheral blood of patients with HT, but only IL-5 was increased in GD. High levels of IL-5 could have resulted in the high titres of antithyroid antibodies and may therefore be considered to play a more significant role than peripheral lymphocytes in the pathogenesis of AITD in the Indian population.
authors.affiliation: Defence Bio-engineering and Electromedical Laboratory, Bangalore, India. gkaustubh@gmail.com
authors.forename: Sripathy
authors.initials: S
authors.lastname: Gopalakrishnan
authors.forename: Sourav
authors.initials: S
authors.lastname: Sen
authors.forename: Jawahar Singh
authors.initials: JS
authors.lastname: Adhikari
authors.forename: Pradeep Kumar
authors.initials: PK
authors.lastname: Chugh
authors.forename: Tarun
authors.initials: T
authors.lastname: Sekhri
authors.forename: Sankar
authors.initials: S
authors.lastname: Rajan
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D019587:Dietary Supplements / Q000009:adverse effects; D005260:Female; D006111:Graves Disease / Q000276:immunology; D050031:Hashimoto Disease / Q000276:immunology; D006801:Humans; D007194:India; D015848:Interleukin-5 / Q000097:blood*; D007455:Iodine / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013961:Thyroid Gland / Q000276:immunology; D013967:Thyroiditis, Autoimmune / Q000209:etiology / Q000276:immunology*; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D015848:Interleukin-5; D007455:Iodine