Static magnetic fields inhibit proliferation and disperse subcellular localization of gamma complex protein3 in cultured C2C12 myoblast cells.
Magnetic fields may delay the rate of cell cycle progression, and there are reports that magnetic fields induce neurite outgrowth in cultured neuronal cells. To demonstrate whether magnetic field also effects on myoblast cells in cell growth, C2C12 cell lines were cultured and 2000G static magnetic field was applied. After 48 h of incubation, both the WST-1 assay (0.01 < P < 0.025, t-test) and direct cell counting (P < 0.0005, t-test) showed that static magnetic fields inhibit the proliferation of cultured C2C12 cells. Immunocytochemistry for alpha and tubulin gamma complex protein (TUBA and GCP3) was made and applying a static magnetic field-dispersed tubulin GCP3 formation, a intracellular apparatus for tubulin structuring in cell division. This protein expression was not altered by western blot. This study indicates that applying a static magnetic field alters the subcellular localizing of GCP3, and may delay the cell growth in cultured C2C12 myoblast cells.
Kim
SeungChan
S
Department of Biotechnology, Yonsei University, Seoul, Korea. synergy@yonsei.ac.kr
Im
Wooseok
W
D000818:Animals; D002452:Cell Count; D002460:Cell Line; D049109:Cell Proliferation; D042541:Intracellular Space / Q000378:metabolism*; D008280:Magnetics; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D032446:Myoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D021381:Protein Transport; D013778:Tetrazolium Salts / Q000494:pharmacology
C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; C550730:GCP3 protein, mouse; D008869:Microtubule-Associated Proteins; D013778:Tetrazolium Salts