title: Establishment of cell suspension cultures of Withania somnifera for the production of withanolide A.
abstract: Cell suspension cultures of Withania somnifera were established in shake flasks and the effect of different growth regulators (auxins, combination of auxin and cytokinin), inoculum density (2.5-20 g L(-1)), different media (MS, B5, NN and N6), the strength of the MS medium (0.25-2.0 x), carbon source (sucrose, glucose, fructose, maltose), concentration of the sucrose (1-8% (w/v)) and the initial pH (4.0-6.5) of the medium were determined for the production of withanolide A. The optimized conditions for biomass accumulation and withanolide A production were found to be 10 g L(-1) of the inoculum on fresh weight basis, the full strength MS medium, 3% (w/v) sucrose, four weeks culture period and the initial medium pH of 5.8. The results of present study are useful for scale-up process.
authors.affiliation: Department of Botany, Karnatak University, Dharwad 580 003, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Nagella
authors.forename: Hosakatte Niranjana
authors.initials: HN
authors.lastname: Murthy
mesh_terms: D018533:Biomass; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D010937:Plant Growth Regulators / Q000494:pharmacology; D032341:Withania / Q000166:cytology / Q000378:metabolism*; D054358:Withanolides
chemical_list: C433551:3-rhamnopyranosyl(1-4)-glucopyranosyl-12-diacetoxy-20-hydroxywitha-5,24-dienolide; D003470:Culture Media; D010937:Plant Growth Regulators; D054358:Withanolides; D004875:Ergosterol