title: Topology and organization of the Salmonella typhimurium type III secretion needle complex components.
abstract: The correct organization of single subunits of multi-protein machines in a three dimensional context is critical for their functionality. Type III secretion systems (T3SS) are molecular machines with the capacity to deliver bacterial effector proteins into host cells and are fundamental for the biology of many pathogenic or symbiotic bacteria. A central component of T3SSs is the needle complex, a multiprotein structure that mediates the passage of effector proteins through the bacterial envelope. We have used cryo electron microscopy combined with bacterial genetics, site-specific labeling, mutational analysis, chemical derivatization and high-resolution mass spectrometry to generate an experimentally validated topographic map of a Salmonella typhimurium T3SS needle complex. This study provides insights into the organization of this evolutionary highly conserved nanomachinery and is the basis for further functional analysis.
authors.affiliation: Research Institute of Molecular Pathology, Vienna, Austria.
authors.forename: Oliver
authors.initials: O
authors.lastname: Schraidt
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Lefebre
authors.forename: Matthias J
authors.initials: MJ
authors.lastname: Brunner
authors.forename: Wolfgang H
authors.initials: WH
authors.lastname: Schmied
authors.forename: Andreas
authors.initials: A
authors.lastname: Schmidt
authors.forename: Julia
authors.initials: J
authors.lastname: Radics
authors.forename: Karl
authors.initials: K
authors.lastname: Mechtler
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Galán
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Marlovits
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D020285:Cryoelectron Microscopy; D046911:Macromolecular Substances; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D012486:Salmonella typhimurium / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D001426:Bacterial Proteins; D046911:Macromolecular Substances; D026901:Membrane Transport Proteins; C079790:PrgH protein, Salmonella typhimurium; C090991:invG protein, Salmonella typhimurium