title: [Effects of mixed carbon sources in cultivation of recombinant Pichia pastoris for polygalacturonate lyase production].
abstract: In order to increase the production and productivity of alkaline polygalacturonate lyase (PGL), we studied the mixed carbon sources feeding strategies during the induction phase by recombinant Pichia pastoris GS 115. Glycerol, sorbitol or lactic acid co-feeding with methanol all enhanced the PGL production. Among all the feeding strategies, the sorbitol co-feeding strategy was most significant. By using this strategy, the PGL activity and productivity reached 1593 U/mL and 16.7 U/(mL-h). Compared to the control, the enhancements of PGL activity and productivity were 84.6% and 45.2% respectively, when we set the sorbitol feeding rate at 3.6 g/(h x L).
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Wang
authors.forename: Dongxu
authors.initials: D
authors.lastname: Zhang
authors.forename: Jianghua
authors.initials: J
authors.lastname: Li
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D002244:Carbon / Q000378:metabolism*; D046508:Culture Techniques; D000432:Methanol / Q000378:metabolism; D010843:Pichia / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D011133:Polysaccharide-Lyases / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D013012:Sorbitol / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D013012:Sorbitol; D002244:Carbon; D011133:Polysaccharide-Lyases; C023911:pectate lyase; D000432:Methanol