title: Proteome analysis of soybean leaves, hypocotyls and roots under salt stress.
abstract: BACKGROUND
  Salinity is one of the most widespread agricultural problems in arid and semi-arid regions that makes fields unproductive, and soil salinization is a serious problem in the entire world. To determine the effects of salt stress on soybean seedlings, a proteomic technique was used.
  
  
  RESULTS
  Soybean plants were exposed to 0, 20, 40, or 80 mM NaCl for one week. The effect of treatment at 20 mM NaCl on plant growth was not severe, at 80 mM NaCl was lethal, and at 40 mM NaCl was significant but not lethal. Based on these results, proteins were extracted from the leaves, hypocotyls and roots of soybean treated with 40 mM NaCl. Nineteen, 22 and 14 proteins out of 340, 330 and 235 proteins in the leaves, hypocotyls and roots, respectively, were up- and down-regulated by NaCl treatment. In leaves, hypocotyls and roots, metabolism related proteins were mainly down-regulated with NaCl treatment. Glyceraldehyde-3-phosphate dehydrogenase was down-regulated in the leaf/hypocotyls, and fructokinase 2 was down-regulated in the hypocotyls/root with NaCl treatment. Stem 31 kDa glycoprotein precursor was up-regulated in all three organs with NaCl treatment. Glyceraldehyde-3-phosphate dehydrogenase was specifically down-regulated at the RNA and protein levels by salt stress.
  
  
  CONCLUSIONS
  These results suggest that metabolism related proteins play a role in each organ in the adaptation to saline conditions.
authors.affiliation: National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp.
authors.forename: Hamid
authors.initials: H
authors.lastname: Sobhanian
authors.forename: Roya
authors.initials: R
authors.lastname: Razavizadeh
authors.forename: Yohei
authors.initials: Y
authors.lastname: Nanjo
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Ehsanpour
authors.forename: Ferdous Rastgar
authors.initials: FR
authors.lastname: Jazii
authors.forename: Nasrin
authors.initials: N
authors.lastname: Motamed
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu