In vitro culture of Keratinocytes from human umbilical cord blood mesenchymal stem cells: the Saigonese culture.
There have been many attempts to acquire and culture human keratinocytes for clinical purposes including from keratotome slices in media with fetal calf serum (FCS) or pituitary extract (PE), from skin specimens in media with feeder layers, from suction blister epidermal roofs' in serum-free culture and from human umbilical cord blood (hUCB) mesenchymal stem cells (MSCs) in media with skin feeder layers. Conversely this study was designed to investigate whether keratinocytes could be obtained directly from hUCB MSCs in vitro. It is widely established that mesenchymal stem cells from human umbilical cord blood have multipotent capacity and the ability to differentiate into disparate cell lineages hUCB MSCs were directly induced to differentiate into keratinocytes by using a specific medium composed of primary culture medium (PCM) and serum free medium (SFM) in a ratio 1:9 for a period of 7 days and tested by immunostain p63 and K1-K10. Cells thus cultured were positive in both tests, confirming the possibility to directly obtain keratinocytes from MSCs hUCB in vitro.
Tran
Cong Toai
CT
Department of Histo-pathology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc Thach Medical University, Ho Chi Minh City, Vietnam. toaiphd@yahoo.com
Huynh
Duy Thao
DT
Gargiulo
Ciro
C
Nguyen
Phuong Thao
PT
Tran
Thi Thanh Thuy
TT
Huynh
Minh Tuan
MT
Nguyen
Thanh Tung
TT
Filgueira
Luis
L
Strong
D Micheal
DM
D018929:Cell Culture Techniques / Q000379:methods*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011247:Pregnancy