2-C-methyluridine modified hammerhead ribozyme against the estrogen receptor.
A new synthesis of 2'-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2'-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5'-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor alpha protein (ER-alpha), and transfection experiments in MCF-7 cell line are also presented.
Pontiggia
Rodrigo
R
INGEBI (CONICET),Vuelta de Obligado 2490-(1428), Buenos Aires, Argentina.
Pontiggia
Osvaldo
O
Simian
Marina
M
Montserrat
Javier M
JM
Engels
Joachim W
JW
Iribarren
Adolfo M
AM
D045744:Cell Line, Tumor; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism*; D014529:Uridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
C516788:2'-C-methyluridine; D009841:Oligonucleotides; D016337:RNA, Catalytic; D012333:RNA, Messenger; D011960:Receptors, Estrogen; C427204:hammerhead ribozyme; C477371:locked nucleic acid; D014529:Uridine