The grape microvine - a model system for rapid forward and reverse genetics of grapevines.
A grapevine model system is described that is suitable for rapid forward and reverse genetic studies in small controlled environments. It is based on the Vvgai1 mutant allele that confers a dwarf stature, short generation cycles and continuous flowering ('microvine'). Black and white berry microvine genotypes were developed that can be transformed by Agrobacterium tumefaciens. Near-homozygous lines were created for efficient bi-allelic single nucleotide polymorphism (SNP) marker mapping and mutagenesis studies. A genetic mapping strategy based on picovine-derived microvine progeny populations was used to rapidly phenotype and map the flower sex and fleshless berry loci and identify a new lethal recessive locus, Vvlrl1. The microvine provides a unique model system for rapid genetic studies of grapevine by changing the perennial long life cycle of the plant to one with features and advantages similar to an annual plant.
Chaïb
Jamila
J
CSIRO Plant Industry, Adelaide Laboratory, Glen Osmond, SA 5064, Australia.
Torregrosa
Laurent
L
Mackenzie
Don
D
Corena
Pat
P
Bouquet
Alain
A
Thomas
Mark R
MR
D000483:Alleles; D002874:Chromosome Mapping / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics; D005638:Fruit / Q000235:genetics; D023281:Genomics / Q000379:methods*; D005838:Genotype; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D014170:Transformation, Genetic; D027843:Vitis / Q000235:genetics*
D018744:DNA, Plant