Influence of chronic renal failure on acid secretion in isolated gastric mucosal cells from human biopsies.
It has been shown previously that the antisecretory response of famotidine (histamine H2-receptor antagonist) is altered in patients with renal failure. To evaluate the underlying mechanism(s) of this clinical observation we obtained biopsy specimens of fundic mucosa from two groups of patients with variable renal function (group 1 normal renal function (n = 8); group 2 renal insufficiency (n = 8), CL(CR) < 20 mL/min) [matched for age and sex]. Furthermore, we investigated the effect of intact parathyroid hormone (PTH (1-84)), urea and calcium on 14C-aminopyrine uptake as an indicator of acid secretion. Gastric mucosal cells from human biopsies were isolated by pronase and collagenase digestion. Cyclic AMP content of parietal cells was determined by radioimmunoassay. Histamine and calcium stimulated 14C-aminopyrine uptake. PTH (1-84) suppressed basal 14C-aminopyrine accumulation, whereas addition of urea had no influence either in presence or in absence of histamine. In contrast to histamine, PTH (1-84) did not induce a significant increase in cellular cyclic AMP. In conclusion, there is no difference in the activation of 14C-aminopyrine uptake between patients with normal renal function and renal insufficiency except lower basal values in patients with renal failure. This could be caused by PTH (1-84) and urea which inhibit gastric acid secretion. Only calcium is the important agent causing an increase in the sensitivity of parietal cells in hyperparathyroidism.
Gladziwa
Ulrich
U
Department of Internal Medicine, University of Witten/Herdecke, Germany. UlrichGladziwa@gmx.de
Gallitzendoerfer
Rainer
R
D000262:Adenylyl Cyclases / Q000378:metabolism; D000632:Aminopyrine / Q000378:metabolism; D001706:Biopsy; D002118:Calcium / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004724:Endoscopy; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000494:pharmacology
D010281:Parathyroid Hormone; D000632:Aminopyrine; D006632:Histamine; D004492:Edetic Acid; D000262:Adenylyl Cyclases; D002118:Calcium