title: APPLICATION OF PREPARATIVE HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY FOR SEPARATION OF ELATINE FROM DELPHINIUM SHAWURENSE.
abstract: Preparative separation of elatine in Delphinium shawurense was achieved for the first time using high-speed countercurrent chromatography (HSCCC). The separation was performed with a solvent system composed of ethyl acetate-chloroform-methanol-water (3:0.1:2:3, v/v) using the lower organic phase as a mobile phase under a revolution speed of 800 rpm. This yielded 72 mg of elatine at over 97% purity with an approximately 95% recovery. The chemical structure was identified by MS and NMR.
authors.affiliation: Xinjiang Key Laboratory of Plant Resources and Natural Products Chemistry, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Dongyu
authors.initials: D
authors.lastname: Gu
authors.forename: Hankui
authors.initials: H
authors.lastname: Wu
authors.forename: Haji Akber
authors.initials: HA
authors.lastname: Aisa
authors.forename: Tianyou
authors.initials: T
authors.lastname: Zhang
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito