title: Collision/reaction cell ICP-MS with shielded torch and sector field ICP-MS for the simultaneous determination of selenium isotopes in biological matrices.
abstract: The determinations of selenium isotopes in biological samples were performed using both inductively coupled plasma collision/reaction cell quadruple mass spectrometer (CRC-ICP-QMS) and inductively coupled plasma sector field mass spectrometers (SF-ICP-MS). To significantly decrease the argon-based interferences at m/z 74 ((36)Ar(38)Ar), 76 ((38)Ar(38)Ar, (40)Ar(36)Ar), 78 ((38)Ar(40)Ar), and 80 ((40)Ar(40)Ar), the gas-flow rates of a helium and hydrogen mixture used in the collision cell were optimized to 1.0 mL/min H(2) and 3.5 mL/min He. Under the optimized condition, the precisions for natural selenium isotope ratio measurements of both instruments were evaluated and compared using 100 ppb Se standard solution. A modified external calibration quantification method was applied for the simultaneous determination of clinically used enriched selinocompounds ((77)Se-selenate, (82)Se-selenite, (76)Se-methylseleninic acid(IV), (78)Se-methylselenonic acid(VI)) and to examine their fate in rat organs (liver, kidney, and lung).
authors.affiliation: Central Laboratories Unit, Qatar University, Doha, Qatar.
authors.forename: Khalid A
authors.initials: KA
authors.lastname: Al-Saad
authors.forename: Mohammed A
authors.initials: MA
authors.lastname: Amr
authors.forename: Abdulfattah I
authors.initials: AI
authors.lastname: Helal
mesh_terms: D000818:Animals; D005740:Gases / Q000737:chemistry; D006371:Helium / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry; D007554:Isotopes / Q000008:administration & dosage / Q000032:analysis*; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage / Q000032:analysis*; D014018:Tissue Distribution
chemical_list: D005740:Gases; D007554:Isotopes; D006371:Helium; D006859:Hydrogen; D012643:Selenium