Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.
Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the approximately 21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes. Phenotypes were scored quantitatively by computational image processing, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival. As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community.
Neumann
Beate
B
MitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Walter
Thomas
T
Hériché
Jean-Karim
JK
Bulkescher
Jutta
J
Erfle
Holger
H
Conrad
Christian
C
Rogers
Phill
P
Poser
Ina
I
Held
Michael
M
Liebel
Urban
U
Cetin
Cihan
C
Sieckmann
Frank
F
Pau
Gregoire
G
Kabbe
Rolf
R
Wünsche
Annelie
A
Satagopam
Venkata
V
Schmitz
Michael H A
MH
Chapuis
Catherine
C
Gerlich
Daniel W
DW
Schneider
Reinhard
R
Eils
Roland
R
Huber
Wolfgang
W
Peters
Jan-Michael
JM
Hyman
Anthony A
AA
Durbin
Richard
R
Pepperkok
Rainer
R
Ellenberg
Jan
J
D000818:Animals; D002455:Cell Division / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D003116:Color; D055785:Gene Knockdown Techniques; D005796:Genes / Q000235:genetics; D015894:Genome, Human / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D008938:Mitosis / Q000235:genetics; D010641:Phenotype; D034622:RNA Interference; D015203:Reproducibility of Results; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism; D013997:Time Factors