title: Redox control in mammalian embryo development.
abstract: The development of an embryo constitutes a complex choreography of regulatory events that underlies precise temporal and spatial control. Throughout this process the embryo encounters ever changing environments, which challenge its metabolism. Oxygen is required for embryogenesis but it also poses a potential hazard via formation of reactive oxygen and reactive nitrogen species (ROS/RNS). These metabolites are capable of modifying macromolecules (lipids, proteins, nucleic acids) and altering their biological functions. On one hand, such modifications may have deleterious consequences and must be counteracted by antioxidant defense systems. On the other hand, ROS/RNS function as essential signal transducers regulating the cellular phenotype. In this context the combined maternal/embryonic redox homeostasis is of major importance and dysregulations in the equilibrium of pro- and antioxidative processes retard embryo development, leading to organ malformation and embryo lethality. Silencing the in vivo expression of pro- and antioxidative enzymes provided deeper insights into the role of the embryonic redox equilibrium. Moreover, novel mechanisms linking the cellular redox homeostasis to gene expression regulation have recently been discovered (oxygen sensing DNA demethylases and protein phosphatases, redox-sensitive microRNAs and transcription factors, moonlighting enzymes of the cellular redox homeostasis) and their contribution to embryo development is critically reviewed.
authors.affiliation: Institute of Biochemistry, University Medicine Berlin-Charité, Berlin, FR Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Ufer
authors.forename: Chi Chiu
authors.initials: CC
authors.lastname: Wang
authors.forename: Astrid
authors.initials: A
authors.lastname: Borchert
authors.forename: Dagmar
authors.initials: D
authors.lastname: Heydeck
authors.forename: Hartmut
authors.initials: H
authors.lastname: Kuhn
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism*; D044127:Epigenesis, Genetic; D018507:Gene Expression Regulation, Developmental; D006706:Homeostasis; D006801:Humans; D010084:Oxidation-Reduction; D014158:Transcription, Genetic