Expression profiling of calcium induced genes in cultured human keratinocytes.
Terminal differentiation of skin keratinocytes is a vertically directed multi-step process that is tightly controlled by the sequential expression of a variety of genes. To examine the gene expression profile in calcium-induced keratinocyte differentiation, we constructed a normalized cDNA library using mRNA isolated from these calcium-treated keratinocytes. After sequencing about 10,000 clones, we were able to obtain 4,104 independent genes. They consisted of 3,699 annotated genes and 405 expressed sequence tags (ESTs). Some were the genes involved in constituting epidermal structures and others were unknown genes that are probably associated with keratinocytes. In particular, we were able to identify genes located at the chromosome 1q21, the locus for the epidermal differentiation complex, and 19q13.1, another probable locus for epidermal differentiation-related gene clusters. One EST located at the chromosome 19q13.1 showed increased expression by calcium treatment, suggesting a novel candidate gene relevant to keratinocyte differentiation. These results demonstrate the complexity of the transcriptional profile of keratinocytes, providing important clues on which to base further investigations of the molecular events underlying keratinocyte differentiation.
Lee
Jung-Suk
JS
Department of Dermatology, School of Medicine, Chungnam National University, Jung-gu, Daejeon, Korea.
Kim
Mi-Rang
MR
Kim
Nam-Soon
NS
Kim
Yong Sung
YS
Yang
Jun-Mo
JM
Cho
Ah Young
AY
Lee
Young
Y
Kim
Chang Deok
CD
Lee
Jeung-Hoon
JH
D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis
D002118:Calcium