Wide field-of-view lens-free fluorescent imaging on a chip.
We demonstrate an on-chip fluorescent detection platform that can simultaneously image fluorescent micro-objects or labeled cells over an ultra-large field-of-view of 2.5 cm x 3.5 cm without the use of any lenses, thin-film filters and mechanical scanners. Such a wide field-of-view lensless fluorescent imaging modality, despite its limited resolution, might be very important for high-throughput screening applications as well as for detection and counting of rare cells within large-area microfluidic devices.
Coskun
Ahmet F
AF
Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.
Su
Ting-Wei
TW
Ozcan
Aydogan
A
D000818:Animals; D002477:Cells; D005456:Fluorescent Dyes; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000473:pathology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods
D005456:Fluorescent Dyes