[Expression and clinical significance of Ang-2 and MMP-7 in laryngeal carcinoma].
OBJECTIVE
To investigate the expression and clinical significance of Ang-2 and MMP-7 protein in laryngeal carcinoma tissue.


METHODS
Immunohistochemical technique was used to detect the expression of Ang-2 and MMP-7 protein in 65 tissues of laryngeal carcinoma and 34 biopsies of adjacent non- cancerous tissue. The relationship between the expression of Ang-2 and MMP-7 and invasion, metastasis or prognosis in laryngeal carcinoma tissue was analyzed.


RESULTS
The positive rates of Ang-2 and MMP-7 were significantly higher in laryngeal carcinoma tissue than those in adjacent non-cancerous tissue (P < 0.05). The level of Ang-2 and MMP-7 expression had no significant correlations with the age and course as well as the smoking, drinking, histological differentiation of carcinoma and clinical classification (P > 0.05). While the expression of Ang-2 significantly differed between patients with different T stage and clinical stage (P < 0.05), and the expression of MMP-7 was notably correlated with the T stage, clinical stage and lymph node metastases (P < 0.05). There was a correlation between the expression of Ang-2 and MMP-7 (P < 0.05). The Kaplan-Meier survival analysis showed that patients with positive expression of Ang-2 had worse overall survival (P < 0.05). However, MMP-7 expression was not related to overall survival or disease-free survival (all P > 0.05). Cox regression analysis indicated that Ang-2 and MMP-7 expression were independent prognostic factors of laryngeal carcinoma.


CONCLUSIONS
Overexpression of Ang-2 and MMP-7 was observed in laryngeal carcinoma and they might be served as an objective indicator for biological behaviour and prognosis.
Qin
Gang
G
Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China. qin-lzm@163.com
Liu
Wenjun
W
Yu
Ling
L
Liang
Zhuoping
Z
Chen
Zuyao
Z
Li
Wanrong
W
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D042702:Angiopoietin-2 / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis
D042702:Angiopoietin-2; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7