title: Common aberrations from the normal human plasma N-glycan profile.
abstract: After performing hydrophilic interaction and weak anion exchange high-performance liquid chromatography to analyze N-glycans in the plasma of 1991 people, we identified several individuals that differed significantly from the "normal" profile of N-glycans. By performing consensus scoring of pairwise distances between vectors containing measured glycan values, we formed six groups of individuals with specific glyco-phenotypes. Some aberrations from the normal plasma protein patterns were found to be associated with clinical conditions (such as renal problems in people with increased monosialylated biantennary glycans, A2G2S1), while other substantial changes in N-glycan structure, such as the near complete absence of neutral glycans or antennary fucosylated tri- and tetraantennary glycans, were not associated with any observed adverse health outcomes. These results demonstrate the existence of specific altered glyco-phenotypes in some individuals and indicate that in some cases they might represent risk factors for the development of specific diseases.
authors.affiliation: Genos Ltd., Glycobiology Division, Planinska 1, 10000 Zagreb, Croatia.
authors.forename: Maja
authors.initials: M
authors.lastname: Pucic
authors.forename: Sofia
authors.initials: S
authors.lastname: Pinto
authors.forename: Mislav
authors.initials: M
authors.lastname: Novokmet
authors.forename: Ana
authors.initials: A
authors.lastname: Knezevic
authors.forename: Olga
authors.initials: O
authors.lastname: Gornik
authors.forename: Ozren
authors.initials: O
authors.lastname: Polasek
authors.forename: Kristian
authors.initials: K
authors.lastname: Vlahovicek
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
authors.forename: Alan F
authors.initials: AF
authors.lastname: Wright
authors.forename: Harry
authors.initials: H
authors.lastname: Campbell
authors.forename: Igor
authors.initials: I
authors.lastname: Rudan
authors.forename: Gordan
authors.initials: G
authors.lastname: Lauc
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011134:Polysaccharides / Q000097:blood*; D012016:Reference Values
chemical_list: D011134:Polysaccharides