title: Association between TNF-alpha, TGF-beta1, IL-10, IL-6 and IFN-gamma gene polymorphisms and generalized aggressive periodontitis.
abstract: OBJECTIVE
  The aim of this study was to investigate links among cytokine genetic variants and generalized aggressive periodontitis (GAgP).
  
  
  METHODS
  Thirty-five patients with generalized aggressive periodontitis and 85 healthy controls without periodontitis were included in the study. Probing depth (PD), clinical attachment loss (CAL), plaque index (PI), and gingival index (GI) were recorded as clinical parameters. Polymorphisms of IL-6, IL-10, IFN-gamma, TGF-ss1 and TNF-alpha gene were analysed using the polymerase chain reaction sequence-specific primer method (PCR-SSP).
  
  
  RESULTS
  No significant differences were observed for IL-6, IL-10, IFN-gamma, and TGF-ss1 cytokine polymorphisms, from the genotype distribution and allele frequency, between GAgP and healthy control groups. In contrast, significant differences were observed in the TNF-alpha gene polymorphism between GAgP and healthy control groups (P = 0.002).
  
  
  CONCLUSIONS
  Our data suggest that TNF-alpha (-308) may be associated with the development of generalized aggressive periodontitis. These results should be replicated in a larger and more diverse population of patients diagnosed with generalized aggressive periodontitis to determine of these findings are generalizable.
authors.affiliation: Department of Periodontology, Gaziantep University Faculty of Dentistry, Gaziantep, 27310, Turkey. erciyas@gantep.edu.tr
authors.forename: Kamile
authors.initials: K
authors.lastname: Erciyas
authors.forename: Sacide
authors.initials: S
authors.lastname: Pehlivan
authors.forename: Tugce
authors.initials: T
authors.lastname: Sever
authors.forename: Mehri
authors.initials: M
authors.lastname: Igci
authors.forename: Ahmet
authors.initials: A
authors.lastname: Arslan
authors.forename: Recep
authors.initials: R
authors.lastname: Orbak
mesh_terms: D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D053773:Transforming Growth Factor beta1 / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014421:Turkey; D055815:Young Adult
chemical_list: D015850:Interleukin-6; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma