title: Fluorescent nano-optodes for glucose detection.
abstract: We have designed fluorescent nanosensors based on ion-selective optodes capable of detecting small molecules. By localizing the sensor components in a hydrophobic core, these nanosensors are able to monitor dynamic changes in concentration of the model analyte, glucose. The nanosensors demonstrated this response in vitro and also when injected subcutaneously into mice. The response of the nanosensors tracked changes in blood glucose levels in vivo that were comparable to measurements taken using a glucometer. The development of these nanosensors offers an alternative, minimally invasive tool for monitoring glucose levels in such fields as diabetes research. Furthermore, the extension of the ion-selective optode sensor platform to small molecule detection will allow for enhanced monitoring of physiological processes.
authors.affiliation: Biomedical Engineering Group, The Charles Stark Draper Laboratory, 555 Technology Square, Cambridge, Massachusetts 02139, USA.
authors.forename: Kelvin
authors.initials: K
authors.lastname: Billingsley
authors.forename: Mary K
authors.initials: MK
authors.lastname: Balaconis
authors.forename: J Matthew
authors.initials: JM
authors.lastname: Dubach
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Ed
authors.initials: E
authors.lastname: Lim
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Francis
authors.forename: Heather A
authors.initials: HA
authors.lastname: Clark
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis* / Q000737:chemistry; D001897:Boronic Acids / Q000737:chemistry; D005456:Fluorescent Dyes; D005947:Glucose / Q000032:analysis*; D051379:Mice; D015394:Molecular Structure; D036103:Nanotechnology
chemical_list: D001786:Blood Glucose; D001897:Boronic Acids; D005456:Fluorescent Dyes; D005947:Glucose