Rad52 SUMOylation affects the efficiency of the DNA repair.
Homologous recombination (HR) plays a vital role in DNA metabolic processes including meiosis, DNA repair, DNA replication and rDNA homeostasis. HR defects can lead to pathological outcomes, including genetic diseases and cancer. Recent studies suggest that the post-translational modification by the small ubiquitin-like modifier (SUMO) protein plays an important role in mitotic and meiotic recombination. However, the precise role of SUMOylation during recombination is still unclear. Here, we characterize the effect of SUMOylation on the biochemical properties of the Saccharomyces cerevisiae recombination mediator protein Rad52. Interestingly, Rad52 SUMOylation is enhanced by single-stranded DNA, and we show that SUMOylation of Rad52 also inhibits its DNA binding and annealing activities. The biochemical effects of SUMO modification in vitro are accompanied by a shorter duration of spontaneous Rad52 foci in vivo and a shift in spontaneous mitotic recombination from single-strand annealing to gene conversion events in the SUMO-deficient Rad52 mutants. Taken together, our results highlight the importance of Rad52 SUMOylation as part of a 'quality control' mechanism regulating the efficiency of recombination and DNA repair.
Altmannova
Veronika
V
Department of Biology, Masaryk University, Brno, Czech Republic.
Eckert-Boulet
Nadine
N
Arneric
Milica
M
Kolesar
Peter
P
Chaloupkova
Radka
R
Damborsky
Jiri
J
Sung
Patrick
P
Zhao
Xiaolan
X
Lisby
Michael
M
Krejci
Lumir
L
D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051135:Rad51 Recombinase / Q000378:metabolism; D051721:Rad52 DNA Repair and Recombination Protein / Q000737:chemistry / Q000378:metabolism*; D011995:Recombination, Genetic; D051716:Replication Protein A / Q000378:metabolism; D025842:SUMO-1 Protein / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
D004277:DNA, Single-Stranded; C497197:RAD52 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D051716:Replication Protein A; D025842:SUMO-1 Protein; D029701:Saccharomyces cerevisiae Proteins; C495151:RAD51 protein, S cerevisiae; D051135:Rad51 Recombinase; D008239:Lysine