[Ion chromatographic analysis and mass spectrometric identification of complexes of metal and ethylenediamine disuccinic acid in plants and soils].
The complexes of metal (Fe (III), Cu, Pb, Zn and Mn) and [S,S']-ethylenediaminedisuccinic acid (EDDS)in plants and soils were determined by using ion chromatography and electrospray ionization mass spectrometry. The separation of metal-EDDS complexes was carried out using the Dionex IonPac AS11-HC column (250 mm x 4.2 mm, 5 microm) with guard column. The mobile phase was 60 mmol/L ammonium nitrate solution at pH 6.5, the flow rate was 1.0 mL/min, and the detection wavelength was 260 nm. The mass spectrometry identification of metal-EDDS complexes was performed by using selected ion monitoring (SIM) mode. The results showed that method can be used for Fe(III)-, Pb-, Cu-EDDS complex determination and the detection limits of Fe(III)-, Pb- and Cu-EDDS complexes were 0.38, 0.54 and 0.18 micromol/L. and the recoveries were 99.6%, 100.6% and 97.5%, respectively. The method is simple, sensitive and suitable for either single or simultaneous analysis of Fe (III)-, Cu- and Pb-EDDS complexes in plants and soils.
Niu
Liyuan
L
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. nly@hist.edu.cn
Shen
Zhenguo
Z
D002845:Chromatography / Q000379:methods*; D009942:Organometallic Compounds / Q000032:analysis*; D010944:Plants / Q000737:chemistry; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013386:Succinates / Q000032:analysis*
D009942:Organometallic Compounds; D012987:Soil; D012989:Soil Pollutants; D013386:Succinates