A synthesized GFP analogue emits via a hydrogen-bonding system.
Two GFP analogues o-HBDI and o-HBMO have been designed and synthesized. The former shows no fluorescence while the latter shows strong fluorescence due to the formation of an intramolecular hydrogen-bond. At low temperatures, both molecules have strong fluorescence.
Kang
Juntao
J
The State Key laboratory of Electroanalytic Chemistry, Changchun Institute of Applied Chemistry, Changchun, 130022, Jilin, China.
Zhao
Guiyan
G
Xu
Jingwei
J
Yang
Wei
W
D001597:Benzylidene Compounds / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D006860:Hydrogen Bonding; D007093:Imidazoles / Q000138:chemical synthesis / Q000737:chemistry*; D010080:Oxazoles / Q000138:chemical synthesis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature
C550727:4-(2-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one; C550728:4-(2-hydroxybenzylidene)-2-methyloxazol-5(4H)-one; D001597:Benzylidene Compounds; D007093:Imidazoles; D010080:Oxazoles; D049452:Green Fluorescent Proteins