Determination of piracetam in rat plasma by LC-MS/MS and its application to pharmacokinetics.
A sensitive and selective liquid chromatography-tandem mass spectrometry method for the determination of piracetam in rat plasma was developed and validated over the concentration range of 0.1-20 µg/mL. After addition of oxiracetam as internal standard, a simplified protein precipitation with trichloroacetic acid (5%) was employed for the sample preparation. Chromatographic separation was performed by a Zorbax SB-Aq column (150 × 2.1 mm, 3.5 µm). The mobile phase was acetonitrile-1% formic acid in water (10:90 v/v) delivered at a flow rate of 0.3 mL/min. The MS data acquisition was accomplished in multiple reaction monitoring mode with a positive electrospray ionization interface. The lower limit of quantification was 0.1 µg/mL. For inter-day and intra-day tests, the precision (RSD) for the entire validation was less than 9%, and the accuracy was within the 94.6-103.2% range. The developed method was successfully applied to pharmacokinetic studies of piracetam in rats following single oral administration dose of 50 mg/kg.
Wang
Xianqin
X
Analytical and Testing Center, Wenzhou Medical College, Wenzhou 325035, China.
Zhu
Jiayin
J
Xu
Renai
R
Yang
Xuezhi
X
Wu
Haiya
H
Lin
Dan
D
Ye
Faqing
F
Hu
Lufeng
L
D000097:Acetonitriles; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D016014:Linear Models; D008297:Male; D010889:Piracetam / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D011759:Pyrrolidines / Q000032:analysis / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000097:Acetonitriles; D011759:Pyrrolidines; C040619:oxiracetam; C032159:acetonitrile; D010889:Piracetam