Association of 22 cytokine gene polymorphisms with tuberculosis in Macedonians.
OBJECTIVE
To examine the possible role of 22 cytokine gene polymorphisms in host susceptibility to or protection against tuberculosis (TB) in Macedonians.


METHODS
301 healthy unrelated individuals and 75 patients with pulmonary TB were studied. Cytokine genotyping was performed by PCR with sequence-specific priming (PCR-SSP) (Heidelberg kit).


RESULTS
TNF-alpha-238/G, IL-1R psti1970/C, IL-1beta + 3962/T:T, IL-4-1098/T:T, IFNgamma utr5644/A:A, IL-10-1082/G:G, IL-4-590/C:C, IL-10/ATC, IL-4/TCT, IL-4/TCC, IL-10/ATC:GCC, IL-4/TCT:TTT, IL-4/TCC:TTC, IL-10/GCC:GCC and IL-4/TCC:TCC were positively associated with TB, while protective association was identified for IL-4-098/G, IL-1beta + 3962/C, IFNgamma utr5644/T, IL-1beta + 3962/C:T, IL-4-1098/G:T, IL-4-590/C:T, IFNgamma utr5644/A:T, IL-4/GCC, IL-4/TTC and IL-4/GCC:TTC.


CONCLUSIONS
These results suggest that some cytokine polymorphisms are significantly associated and affect host susceptibility/resistance to TB in Macedonians.
Trajkov
Dejan
D
Institute of Immunobiology and Human Genetics, Faculty of Medicine, University St. Kiril and Metodij, Skopje, Republic of Macedonia.
Trajchevska
Mirjana
M
Arsov
Todor
T
Petlichkovski
Aleksandar
A
Strezova
Ana
A
Efinska-Mladenovska
Olivija
O
Sandevski
Aleksandar
A
Spiroski
Mirko
M
D000328:Adult; D016207:Cytokines / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006115:Greece; D006239:Haplotypes; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics; D007378:Interleukins / Q000235:genetics; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014376:Tuberculosis / Q000453:epidemiology*
D016207:Cytokines; D007376:Interleukin-2; D007378:Interleukins