title: Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures.
abstract: A shortened protocol for two peptide nucleic acid fluorescence in situ hybridization (PNA FISH) assays for the detection of Gram-negative bacilli from positive blood cultures was evaluated in a multicenter trial. There was 100% concordance between the two protocols for each assay (368 of 368 and 370 of 370 results) and 99.7% (367 of 368 and 369 of 370 results) agreement with routine laboratory techniques.
authors.affiliation: Cedars Sinai Medical Center, Los Angeles, California, USA.
authors.forename: Margie
authors.initials: M
authors.lastname: Morgan
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Marlowe
authors.forename: Phyllis
authors.initials: P
authors.lastname: Della-Latta
authors.forename: Hossein
authors.initials: H
authors.lastname: Salimnia
authors.forename: Susan
authors.initials: S
authors.lastname: Novak-Weekley
authors.forename: Fann
authors.initials: F
authors.lastname: Wu
authors.forename: Benjamin S
authors.initials: BS
authors.lastname: Crystal
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D020135:Peptide Nucleic Acids; D012680:Sensitivity and Specificity
chemical_list: D020135:Peptide Nucleic Acids