title: Pseudonocardia rhizophila sp. nov., a novel actinomycete isolated from a rhizosphere soil.
abstract: During the course of our research on new actinobacterial sources, a novel actinomycete strain YIM 67013(T) was isolated from rhizosphere soil of Tripterygium wilfordii Hook. f. collected from Yunnan province, south-west China. The strain formed well-differentiated aerial and substrate mycelia, grew in the presence of up to 15% NaCl (optimum, 0-3% NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 67013(T) belongs to the genus Pseudonocardia, with highest similarity to Pseudonocardia thermophila DSM 43832(T) (97.6%). Sequence similarities between strain YIM 67013(T) and the other Pseudonocardia species ranged from 96.6 to 93.2%. Key morphological and physiological characteristics as well as chemotaxonomic features of strain YIM 67013(T) were congruent with the description of the genus Pseudonocardia. The G+C content of the genomic DNA was 69.7 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including low DNA-DNA hybridization results, it is proposed that strain YIM 67013(T) represents a novel species of the genus Pseudonocardia, named Pseudonocardia rhizophila sp. nov. The type strain is YIM 67013(T) (= CCTCC AA 209043(T) = DSM 45381(T)).
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, Yunnan, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Guo-Zhen
authors.initials: GZ
authors.lastname: Zhao
authors.forename: Hai-Yu
authors.initials: HY
authors.lastname: Huang
authors.forename: Wen-Yong
authors.initials: WY
authors.lastname: Zhu
authors.forename: Jae-Chan
authors.initials: JC
authors.lastname: Lee
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Zhang
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S