[Expression of nuclear autoantigenic sperm protein in platinum resistance ovarian cancer cell clones].
OBJECTIVE
To study the expression of NASP in platinum resistance ovarian cancer cell clones (C0C1/DDP).


METHODS
The proteins of C0C1 and C0C1/DDP were separated by highly sensitive two-dimensional differential gel electrophoresis (2-D DIGE) coupled with matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF-MS) to identify the proteins differentially expressed in cisplatin sensitive and cisplatin resistance cell lines.


RESULTS
The investigators found a group of the distinct expressed proteins between C0C1 and C0C1/DDP. Among them, NASP was one of the down-regulated proteins by 21% (Student's t-test; P = 0.01).


CONCLUSIONS
The identification of NASP in C0C1/DDP has laid a foundation for future in-depth researches into the mechanisms of ovarian cancer platinum resistance.
Wang
Hui-xiang
HX
Department of Obstetrics and Gynecology, Affiliated Yuquan Hospital of Tsinghua University, Beijing 100049, China.
Tan
Yan
Y
D001324:Autoantigens / Q000378:metabolism*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology* / Q000627:therapeutic use; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000378:metabolism*
D001324:Autoantigens; C489390:NASP protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D002945:Cisplatin