Multi wall carbon nanotubes induce oxidative stress and cytotoxicity in human embryonic kidney (HEK293) cells.
The present study was aimed at evaluating the potential toxicity and the general mechanism involved in multi wall carbon nanotubes (MWCNT)-induced cytotoxicity using human embryonic kidney cell line (HEK293) cells. Two multi wall carbon nanotubes (coded as MWCNT1, size: 90-150nm and MWCNT2, size: 60-80nm) used in this study are MWCNT1 (produced by the electric arc method and size of the nanotubes was 90-150nm) and MWCNT2 (produced by the chemical vapor deposition method with size of 60-80nm). To elucidate the possible mechanisms of MWCNT induced cytotoxicity, cell viability, mitochondrial function (MTT assay), cell membrane damage (LDH assay), reduced glutathione (GSH), interleukin-8 (IL-8) and lipid peroxidation levels were quantitatively assessed under carbon nanotubes exposed (48h) conditions. Exposure of different sizes of two carbon nanotubes at dosage levels between 3 and 300mug/ml decreased cell viability in a concentration dependent manner. The IC(50) values (concentration of nanoparticles to induce 50% cell mortality) of two (MWCNT1, MWCNT2) nanoparticles were found as 42.10 and 36.95mug/ml. Exposure of MWCNT (10-100mug/ml) to HEK cells resulted in concentration dependent cell membrane damage (as indicated by the increased levels of LDH), increased production of IL-8, increased TBARS and decreased intracellular glutathione levels. The cytotoxicity and oxidative stress was significantly more in MWCNT2 exposed cells than MWCNT1. In summary, exposure of carbon nanotubes resulted in a concentration dependent cytotoxicity in cultured HEK293 cells that was associated with increased oxidative stress.
Reddy
Anreddy Rama Narsimha
AR
Department of Pharmacology and Toxicology, University College of Pharmaceutical Sciences, Kakatiya University, Warangal 506 009, India.
Reddy
Yellu Narsimha
YN
Krishna
Devarakonda Rama
DR
Himabindu
Vurimindi
V
D002244:Carbon / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003593:Cytoplasm / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism; D053758:Nanoparticles / Q000633:toxicity*; D037742:Nanotubes, Carbon / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010087:Oxides / Q000378:metabolism
D016209:Interleukin-8; D037742:Nanotubes, Carbon; D010087:Oxides; D002244:Carbon; D007770:L-Lactate Dehydrogenase; D005978:Glutathione