Continuous sorting of heterogeneous-sized embryoid bodies.
This paper presents a microfluidic device for sorting embryoid bodies (EBs) with large dynamic size ranges up to 300 microm. The proposed separation scheme utilizes appropriately spaced pillars within a microchannel to alter the fluid flow pathway, thus allowing particles of defined sizes to be diverted towards specific flow paths. We test the device functionality by separating polystyrene beads 90, 175 and 275 microm in diameter, demonstrating separation efficiencies approaching 100%. We then demonstrate for the first time on-chip separation of mouse EBs, which were separated into three size groups. The ability to extract specific size ranges of EBs will greatly facilitate their subsequent differentiation studies.
Lillehoj
Peter B
PB
Mechanical and Aerospace Engineering Department, University of California, Los Angeles, CA, USA.
Tsutsui
Hideaki
H
Valamehr
Bahram
B
Wu
Hong
H
Ho
Chih-Ming
CM
D000818:Animals; D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*