LINE-1 methylation difference between ameloblastoma and keratocystic odontogenic tumor.
OBJECTIVE
Global hypomethylation is a common epigenetic event in cancer. Keratocystic odontogenic tumor (KCOT) and ameloblastoma are different tumors but posses the same tissue in origin. Here, we investigated long interspersed nuclear element-1 (LINE-1 or L1) methylation status between ameloblastoma and KCOT.


METHODS
We studied the methylation levels of the long interspersed nucleotide element-1 (LINE-1) in ameloblastoma and KCOT. After collecting ameloblastoma cells and epithelium lining cells of KCOT by laser capture microdissection from paraffin embedded tissue, combined bisulfite restriction analysis of LINE-1 (COBRALINE-1) was performed to measure LINE-1 methylation levels.


RESULTS
The LINE-1 methylation level in KCOT (53.16 +/- 12.03%) was higher than that in ameloblastoma (36.90 +/- 16.52%), with a statistical significance of P = 0.001. The ranges of LINE-1 methylation of both lesions were not associated with either age or sex.


CONCLUSIONS
We found LINE-1 hypomethylation levels between ameloblastoma and KCOT are different. Therefore, global methylations between these tumors are processed differently.
Kitkumthorn
N
N
Department of Oral Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand. nakarinkit@hotmail.com
Mutirangura
A
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000564:Ameloblastoma / Q000737:chemistry / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002648:Child; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D007573:Jaw Neoplasms / Q000737:chemistry / Q000235:genetics*; D007633:Keratins; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009808:Odontogenic Tumors / Q000737:chemistry / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping / Q000379:methods; D055815:Young Adult
D004273:DNA, Neoplasm; D007633:Keratins