title: Comparative gene expression analysis of susceptible and resistant near-isogenic lines in common wheat infected by Puccinia triticina.
abstract: Gene expression after leaf rust infection was compared in near-isogenic wheat lines differing in the Lr10 leaf rust resistance gene. RNA from susceptible and resistant plants was used for cDNA library construction. In total, 55 008 ESTs were sequenced from the two libraries, then combined and assembled into 14 268 unigenes for further analysis. Of these ESTs, 89% encoded proteins similar to (E value of < or =10(-5)) characterized or annotated proteins from the NCBI non-redundant database representing diverse molecular functions, cellular localization and biological processes based on gene ontology classification. Further, the unigenes were classified into susceptible and resistant classes based on the EST members assembled from the respective libraries. Several genes from the resistant sample (14-3-3 protein, wali5 protein, actin-depolymerization factor and ADP-ribosylation factor) and the susceptible sample (brown plant hopper resistance protein, caffeic acid O-methyltransferase, pathogenesis-related protein and senescence-associated protein) were selected and their differential expression in the resistant and susceptible samples collected at different time points after leaf rust infection was confirmed by RT-PCR analysis. The molecular pathogenicity of leaf rust in wheat was studied and the EST data generated made a foundation for future studies.
authors.affiliation: Kihara Institute for Biological Research, Yokohama City University, Yokohama 244-0813, Japan.
authors.forename: Alagu
authors.initials: A
authors.lastname: Manickavelu
authors.forename: Kanako
authors.initials: K
authors.lastname: Kawaura
authors.forename: Kazuko
authors.initials: K
authors.lastname: Oishi
authors.forename: Tadasu
authors.initials: T
authors.lastname: Shin-I
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kohara
authors.forename: Nabila
authors.initials: N
authors.lastname: Yahiaoui
authors.forename: Beat
authors.initials: B
authors.lastname: Keller
authors.forename: Ayako
authors.initials: A
authors.lastname: Suzuki
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yano
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Ogihara
mesh_terms: D001487:Basidiomycota / Q000235:genetics / Q000472:pathogenicity*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods*; D015966:Gene Expression Regulation, Fungal; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D014908:Triticum / Q000235:genetics* / Q000382:microbiology*