title: Livestock-associated methicillin-resistant Staphylococcus aureus sequence type 398 in humans, Canada.
abstract: Rates of colonization with livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) sequence type 398 have been high for pigs and pig farmers in Canada, but prevalence rates for the general human population are unknown. In this study, 5 LA-MRSA isolates, 4 of which were obtained from skin and soft tissue infections, were identified from 3,687 tested MRSA isolates from persons in Manitoba and Saskatchewan, Canada. Further molecular characterization determined that these isolates all contained staphylococcal cassette chromosome (SCC) mecV, were negative for Panton-Valentine leukocidin, and were closely related by macrorestriction analysis with the restriction enzyme Cfr91. The complete DNA sequence of the SCCmec region from the isolate showed a novel subtype of SCCmecV harboring clustered regularly interspaced short palindromic repeats and associated genes. Although prevalence of livestock-associated MRSA seems to be low for the general population in Canada, recent emergence of infections resulting from this strain is of public health concern.
authors.affiliation: National Microbiology Laboratory, Winnipeg, Manitoba, Canada.
authors.forename: George R
authors.initials: GR
authors.lastname: Golding
authors.forename: Louis
authors.initials: L
authors.lastname: Bryden
authors.forename: Paul N
authors.initials: PN
authors.lastname: Levett
authors.forename: Ryan R
authors.initials: RR
authors.lastname: McDonald
authors.forename: Alice
authors.initials: A
authors.lastname: Wong
authors.forename: John
authors.initials: J
authors.lastname: Wylie
authors.forename: Morag R
authors.initials: MR
authors.lastname: Graham
authors.forename: Shaun
authors.initials: S
authors.lastname: Tyler
authors.forename: Gary
authors.initials: G
authors.lastname: Van Domselaar
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Simor
authors.forename: Denise
authors.initials: D
authors.lastname: Gravel
authors.forename: Michael R
authors.initials: MR
authors.lastname: Mulvey
mesh_terms: D000368:Aged; D000818:Animals; D000829:Animals, Domestic / Q000821:virology; D001427:Bacterial Toxins / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D005098:Exotoxins / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D055029:Inverted Repeat Sequences / Q000235:genetics; D007956:Leukocidins / Q000235:genetics; D008297:Male; D008350:Manitoba / Q000453:epidemiology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D008875:Middle Aged; D016254:Mutagenesis, Insertional / Q000235:genetics; D015995:Prevalence; D012525:Saskatchewan / Q000453:epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology; D013552:Swine / Q000382:microbiology
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific