title: A software tool to accelerate design of protein constructs for recombinant expression.
abstract: Structural and biochemical analysis of proteins requires access to purified protein material. Modern molecular biology technologies facilitate straightforward molecular cloning and expression analysis of multiple protein constructs in parallel, and such approaches have proven very efficient to identify samples suitable for further analysis. A variety of information can be used to support rational design of protein constructs. This includes, e.g. prediction of secondary structure elements, protein domain predictions, and structure prediction methods such as threading. To fully access the available information, collation of data extracted from several different sources is required. This can be cumbersome and sometimes also confusing due to for example different implementation of amino acid residue numbering schemes. The SGC Domain Boundary Analyser tool provides a graphical interface that simplifies and accelerates rational design of protein expression constructs.
authors.affiliation: Structural Genomics Consortium, Karolinska Institutet, Department of Medical Biochemistry and Biophysics, 171 77 Stockholm, Sweden.
authors.forename: Johanna
authors.initials: J
authors.lastname: Sagemark
authors.forename: Per
authors.initials: P
authors.lastname: Kraulis
authors.forename: Johan
authors.initials: J
authors.lastname: Weigelt
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D019295:Computational Biology / Q000379:methods; D008969:Molecular Sequence Data; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods*; D012984:Software; D014584:User-Computer Interface
chemical_list: D011994:Recombinant Proteins