title: Rapid profiling and target analysis of principal components in Fuling Decoctions by UFLC-DAD-ESI-MS.
abstract: The traditional Chinese medicine formula Fuling Decoction (FD) has been clinically used for eczema treatment, but the unclear chemical distribution and the lack of quality control have strongly restricted its application. In this study, an analytical method incorporating ultra-fast liquid chromatography (UFLC) with MS and UV detection was developed for rapid profiling of the chemical constitutes from FD. Fourteen constitutes were identified by UFLC-ESI-MS, while four major components including genipingentiobioside, geniposide, paeoniflorin and liquiritin were quantified simultaneously by UFLC-DAD. The UFLC-based method was fully validated and can be applied to screening and determination of principal components in commercially FD prescriptions.
authors.affiliation: The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Ao-Xue
authors.initials: AX
authors.lastname: Wang
authors.forename: Guang-Bo
authors.initials: GB
authors.lastname: Ge
authors.forename: Xiao-Yi
authors.initials: XY
authors.lastname: Qi
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.forename: Si-Cheng
authors.initials: SC
authors.lastname: Liang
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Tu
mesh_terms: D001565:Benzoates / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D044950:Flavanones / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D039823:Iridoids / Q000032:analysis*; D039821:Monoterpenes; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; D039823:Iridoids; D039821:Monoterpenes; C007835:geniposide; C015423:peoniflorin; D006027:Glycosides; C058967:genipin 1-gentiobioside; C512196:liquiritin