Determination of brominated phenols in water samples by on-line coupled isotachophoresis with capillary zone electrophoresis.
A method for determination of nine brominated phenols as environmental risk compounds was developed by on-line coupled capillary isotachophoresis and capillary zone electrophoresis (ITP-CZE). For ITP step, 1x10(-2) mol L(-1) hydrochloric acid with 3x10(-2) mol L(-1) ammediol pH 9.1 was used as the leading electrolyte, and 3x10(-2) mol L(-1) beta-alanine with 2x10(-2) mol L(-1) sodium hydroxide pH 10.05 was used as the terminating electrolyte. As the background electrolyte for CZE separation, 2.5x10(-2) mol L(-1) beta-alanine with 2.5x10(-2) mol L(-1) lysine pH 9.6 was used. All electrolytes contained 0.05% or 0.1% (m/v) hydroxyethylcellulose to suppress the electroosmotic flow. UV detection at wavelength 220 nm was used. Detection limits in order of tens of nmol L(-1) were achieved. Good repeatability of migration times (less than 0.33% RSD) and good repeatability of peak areas (less than 7.19% RSD) at concentration level 5x10(-8) mol L(-1) were observed. Developed ITP-CZE method was applied to determination of brominated phenols in spiked tap and river water samples.
Knob
Radim
R
Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, Tr. 17. Listopadu 12, Olomouc, CZ-77146, Czech Republic. rknob@seznam.cz
Marák
Jozef
J
Stanová
Andrea
A
Maier
Vítezslav
V
Kaniansky
Dusan
D
Sevcík
Juraj
J
D004586:Electrophoresis / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006842:Hydrocarbons, Brominated / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D014874:Water Pollutants, Chemical / Q000032:analysis*
D006842:Hydrocarbons, Brominated; D010636:Phenols; D014874:Water Pollutants, Chemical