Delayed chromosomal instability in lymphocytes of cancer patients after radiotherapy.
OBJECTIVE
To assess possible delayed chromosomal instability (DCI) expressed as elevated chromatid breakage in cells containing previously formed chromosome type aberrations in cultured blood lymphocytes of cancer patients after radiotherapy (RT).


METHODS
Twenty patients treated for uterine cancer with external Co(60) RT, without chemotherapy, were selected. Blood was taken before, 1-2 days after RT and one year later. Lymphocytes were cultured for 50 and 100 h. Metaphases were stained with fluorescence-plus-Giemsa and analysed for chromosome and chromatid aberrations in 1st (M1) and 3rd plus later (M3+) mitoses.


RESULTS
RT caused a significant increase of radiation-specific chromosome aberrations in patients' lymphocytes together with DCI, which was observed as an excessive yield of cells containing both chromosome and chromatid aberrations (defined as C(acs&act)). This DCI passed successfully through mitoses in vitro, and at the end of RT a mean yield of 'extra' C(acs&act) was 3 x 10(-3) x cell(-1) amongst either M1 or M3+ cells. At the end of RT and one year later DCI in M1 lymphocytes appeared at random amongst patients, but some inter-individual variation was found for DCI presence in M3+ cells at both post-irradiation samplings. As time passed, the mean yield of lymphocytes exhibiting DCI decreased in vivo and one year after RT reached the pre-treatment level of 1 x 10(-3) x cell(-1).


CONCLUSIONS
DCI was demonstrated in descendants of human lymphocytes after therapeutic irradiation. The effect diminished one year later, suggesting that the progeny of patients' irradiated stem cells did not produce new daughter lymphocytes exhibiting DCI during the studied post-irradiation period.
Vinnikov
Volodymyr A
VA
Radiation Cytogenetics Laboratory, Institute for Medical Radiology AMS of Ukraine, Kharkiv, Ukraine. lrcg.imr@mail.ru
Maznyk
Nataliya A
NA
Lloyd
David
D
D000368:Aged; D043171:Chromosomal Instability / Q000528:radiation effects*; D002869:Chromosome Aberrations / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D008875:Middle Aged; D008938:Mitosis / Q000528:radiation effects; D011877:Radionuclide Imaging; D013997:Time Factors; D014594:Uterine Neoplasms / Q000000981:diagnostic imaging / Q000235:genetics* / Q000378:metabolism / Q000532:radiotherapy*