[Determination of malachite green and leukomalachite green residues in fish muscle by high-performance liquid chromatography method].
Malachite green (MG) and leukomalachite green (LMG) are subjected to monitoring fish muscle, with a minimum required performance limit (MRPL) set 2 microg/kg. Samples were extracted with acetonitryle-buffer mixture and cleaned up on SCX solid phase extraction (SPE) column. LC separation MG and LMG was done on column Luna Phenyl-Hexyl Phenomenex. Samples were fortified with MG and LMG between 2 - 25 microg/kg. The coefficients of variation (CV%) were lower than 14% for MG and 16%for LMG. The mean recoveries were in the range 65- 83% for MG and 70- 73% for LMG. This method fulfils the criteria for identification and determination of MG andLMG residues in the samples of fish muscle.
Doroszkiewicz-Fiedoruk
Jelena
J
Pracownia Badań Chemicznych Srodków Spozywczych, Zakład Higieny Weterynaryjnej, Białystok. fiedoruk@wiw.bianet.com.pl
Masłowiecka
Jolanta
J
Rodziewicz
Lech
L
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005399:Fishes; D005659:Fungicides, Industrial / Q000032:analysis; D018482:Muscle, Skeletal / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D012394:Rosaniline Dyes / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
D005659:Fungicides, Industrial; D010573:Pesticide Residues; D012394:Rosaniline Dyes; C005095:malachite green; C015320:leucomalachite green