Antibacterial effect of the bioactive compound beauvericin produced by Fusarium proliferatum on solid medium of wheat.
To obtain the bioactive compound beauvericin (BEA), Fusarium proliferatum CECT 20569 was grown on a solid medium of wheat, utilizing the technique of the solid state fermentation (SSF), being this mycotoxin purified by high performance liquid chromatography (HPLC) with a reverse phase semi-preparative column using as the mobile phase acetonitrile/water in gradient condition. The purity of the BEA was verified by analytical HPLC and liquid chromatography tandem mass spectrometry (LC/MS-MS). The pure fractions of BEA were utilized to determinate the antibiotic effects on several bacterial strains that are considered normally pathogens of the intestinal tract as: Escherichia coli, Enterococcus faecium, Salmonella enterica, Shigella dysenteriae, Listeria monocytogenes, Yersinia enterocolitica, Clostridium perfringens, Pseudomonas aeruginosa and Staphylococcus aureus.
Meca
G
G
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain. giuseppe.meca@uv.es
Sospedra
I
I
Soriano
J M
JM
Ritieni
A
A
Moretti
A
A
Mañes
J
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002853:Chromatography, Liquid; D047630:Depsipeptides / Q000494:pharmacology*; D005670:Fusarium / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000378:metabolism* / Q000382:microbiology
D000900:Anti-Bacterial Agents; D047630:Depsipeptides; C004456:beauvericin