Rapid linkage of indole carboxylic acid to the plant cell wall identified as a component of basal defence in Arabidopsis against hrp mutant bacteria.
Changes occurring to plant cell walls were examined following inoculation of Arabidopsis leaves with pathogenic and non-pathogenic (hrpA mutant) strains of Pseudomonas syringae pv. tomato. We have targeted low molecular weight, cross-linked phenolic and indolic compounds that were released from wall preparations by alkaline hydrolysis at 70 degrees C and in a microwave bomb. Significantly higher concentrations of syringaldehyde, p hydroxybenzaldehyde and indole carboxylic acid were recovered from cell walls isolated from leaves 24h after challenge with the hrpA mutant compared with wild-type DC3000. Time course experiments showed that the accumulation of indole carboxylic acid and the other group of differentiating metabolites had occurred within 12h of inoculation. The callose synthase deficient mutant pmr4-1 was more resistant than wild-type Columbia plants to P. syringae pv. tomato. Restricted bacterial multiplication was associated with increased accumulation of indole carboxylic acid on the plant cell wall. In the absence of callose deposition in the pmr 4-1 mutant, indolic derivatives may serve as a structural scaffold for wall modifications following bacterial challenge.
Forcat
Silvia
S
Division of Biology, Imperial College London, London, UK.
Bennett
Mark
M
Grant
Murray
M
Mansfield
John W
JW
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D001547:Benzaldehydes / Q000032:analysis; D002264:Carboxylic Acids / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000378:metabolism; D044224:Pseudomonas syringae / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D001547:Benzaldehydes; D002264:Carboxylic Acids; D007211:Indoles; C069665:syringaldehyde; C011483:4-hydroxybenzaldehyde