title: Digital gene expression by tag sequencing on the illumina genome analyzer.
abstract: This unit provides a protocol for performing digital gene expression profiling on the Illumina Genome Analyzer sequencing platform. Tag sequencing (Tag-seq) is an implementation of the LongSAGE protocol on the Illumina sequencing platform that increases utility while reducing both the cost and time required to generate gene expression profiles. The ultra-high-throughput sequencing capability of the Illumina platform allows the cost-effective generation of libraries containing an average of 20 million tags, a 200-fold improvement over classical LongSAGE. Tag-seq has less sequence composition bias, leading to a better representation of AT-rich tag sequences, and allows a more accurate profiling of a subset of the transcriptome characterized by AT-rich genes expressed at levels below the threshold of detection of LongSAGE (Morrissy et al., 2009).
authors.affiliation: BCCA Genome Sciences Centre, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Sorana
authors.initials: S
authors.lastname: Morrissy
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Allen
authors.initials: A
authors.lastname: Delaney
authors.forename: Jennifer
authors.initials: J
authors.lastname: Asano
authors.forename: Noreen
authors.initials: N
authors.lastname: Dhalla
authors.forename: Irene
authors.initials: I
authors.lastname: Li
authors.forename: Helen
authors.initials: H
authors.lastname: McDonald
authors.forename: Pawan
authors.initials: P
authors.lastname: Pandoh
authors.forename: Anna-Liisa
authors.initials: AL
authors.lastname: Prabhu
authors.forename: Angela
authors.initials: A
authors.lastname: Tam
authors.forename: Martin
authors.initials: M
authors.lastname: Hirst
authors.forename: Marco
authors.initials: M
authors.lastname: Marra
mesh_terms: D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D023281:Genomics / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D012333:RNA, Messenger