title: Effect of cooking methods on fatty acids, conjugated isomers of linoleic acid and nutritional quality of beef intramuscular fat.
abstract: The effect of boiling, microwaving and grilling on the composition and nutritional quality of beef intramuscular fat from cattle fed with two diets was investigated. Longissimus lumborum muscle from 15 Alentejano young bulls fed on concentrate or pasture was analyzed. Cooking losses and, consequently, total lipids, increased directly with the cooking time and internal temperature reached by meat (microwaving>boiling>grilling). The major changes in fatty acid composition, which implicated 16 out of 34 fatty acids, resulted in higher percentages in cooked beef of SFA and MUFA and lower proportions of PUFA, relative to raw meat, while conjugated linoleic acid (CLA) isomers revealed a great stability to thermal processes. Heating decreased the PUFA/SFA ratio of meat but did not change its n-6/n-3 index. Thermal procedures induced only slight oxidative changes in meat immediately after treatment but hardly affected the true retention values of its individual fatty acids (72-168%), including CLA isomers (81-128%).
authors.affiliation: CIISA, Faculdade de Medicina Veterinária, Avenida da Universidade Técnica, Pólo Universitário do Alto da Ajuda, 1300-477 Lisboa, Portugal.
authors.forename: Cristina M M
authors.initials: CM
authors.lastname: Alfaia
authors.forename: Susana P
authors.initials: SP
authors.lastname: Alves
authors.forename: Anabela F
authors.initials: AF
authors.lastname: Lopes
authors.forename: Maria J E
authors.initials: MJ
authors.lastname: Fernandes
authors.forename: Ana S H
authors.initials: AS
authors.lastname: Costa
authors.forename: Carlos M G A
authors.initials: CM
authors.lastname: Fontes
authors.forename: Matilde L F
authors.initials: ML
authors.lastname: Castro
authors.forename: Rui J B
authors.initials: RJ
authors.lastname: Bessa
authors.forename: José A M
authors.initials: JA
authors.lastname: Prates
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry* / Q000502:physiology; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002417:Cattle; D003296:Cooking; D004032:Diet / Q000662:veterinary; D005227:Fatty Acids / Q000737:chemistry*; D044243:Linoleic Acids, Conjugated / Q000737:chemistry*; D008297:Male; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000502:physiology; D009753:Nutritive Value
chemical_list: D005227:Fatty Acids; D044243:Linoleic Acids, Conjugated