Promoter diversity in multigene transformation.
Multigene transformation (MGT) is becoming routine in plant biotechnology as researchers seek to generate more complex and ambitious phenotypes in transgenic plants. Every nuclear transgene requires its own promoter, so when coordinated expression is required, the introduction of multiple genes leads inevitably to two opposing strategies: different promoters may be used for each transgene, or the same promoter may be used over and over again. In the former case, there may be a shortage of different promoters with matching activities, but repetitious promoter use may in some cases have a negative impact on transgene stability and expression. Using illustrative case studies, we discuss promoter deployment strategies in transgenic plants that increase the likelihood of successful and stable multiple transgene expression.
Peremarti
Ariadna
A
Departament de Producció Vegetal i Ciència Forestal, ETSEA, Universitat de Lleida, Av. Alcalde Rovira Roure 191, 25198 Lleida, Spain.
Twyman
Richard M
RM
Gómez-Galera
Sonia
S
Naqvi
Shaista
S
Farré
Gemma
G
Sabalza
Maite
M
Miralpeix
Bruna
B
Dashevskaya
Svetlana
S
Yuan
Dawei
D
Ramessar
Koreen
K
Christou
Paul
P
Zhu
Changfu
C
Bassie
Ludovic
L
Capell
Teresa
T
D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013997:Time Factors; D014170:Transformation, Genetic / Q000235:genetics*