title: Functional vanilloid receptor-1 in human submandibular glands.
abstract: Vanilloid receptor-1 (VR1) was originally found in the nervous system. Recent evidence indicates that VR1 is also expressed in various cell types. We hypothesized that VR1 exists in the human submandibular gland (SMG) and is involved in regulating salivary secretion. VR1 mRNA and protein were expressed in human SMGs and a human salivary intercalated duct cell line. VR1 was mainly located in serous acinar and ductal cells, but not in mucous acinar cells. Capsaicin, an agonist of VR1, increased intracellular free calcium, enhanced phosphorylation of extracellular signal-regulated kinase, and induced the trafficking of aquaporin 5 (AQP5) from the cytoplasm to the plasma membrane. These effects were abolished by pre-treatment with the VR1 antagonist capsazepine. Furthermore, capsaicin cream applied to the skin covering the submandibular area increased salivary secretion. These findings indicated that a functional VR1 is expressed in the human SMG and is involved in regulating salivary secretion by mediating AQP5 trafficking.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, 22 Zhong Guan Cun South St., Beijing, 100081, PR China.
authors.forename: Q W
authors.initials: QW
authors.lastname: Ding
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: Y N
authors.initials: YN
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: C
authors.initials: C
authors.lastname: Ding
authors.forename: L L
authors.initials: LL
authors.lastname: Wu
authors.forename: G Y
authors.initials: GY
authors.lastname: Yu
mesh_terms: D000328:Adult; D000368:Aged; D051402:Aquaporin 5 / Q000187:drug effects; D002118:Calcium / Q000032:analysis; D002211:Capsaicin / Q000031:analogs & derivatives / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D003593:Cytoplasm / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000166:cytology; D010766:Phosphorylation; D012463:Saliva / Q000187:drug effects / Q000378:metabolism; D018987:Salivary Ducts / Q000166:cytology; D012704:Serous Membrane / Q000166:cytology; D013363:Submandibular Gland / Q000166:cytology*; D050916:TRPV Cation Channels / Q000819:agonists / Q000032:analysis* / Q000037:antagonists & inhibitors; D055815:Young Adult
chemical_list: C496036:AQP5 protein, human; D051402:Aquaporin 5; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; D048049:Extracellular Signal-Regulated MAP Kinases; C071423:capsazepine; D002211:Capsaicin; D002118:Calcium