title: Survey of transcripts expressed by the invasive juvenile stage of the liver fluke Fasciola hepatica.
abstract: BACKGROUND
  The common liver fluke Fasciola hepatica is the agent of a zoonosis with significant economic consequences in livestock production worldwide, and increasing relevance to human health in developing countries. Although flukicidal drugs are available, re-infection and emerging resistance are demanding new efficient and inexpensive control strategies. Understanding the molecular mechanisms underlying the host-parasite interaction provide relevant clues in this search, while enlightening the physiological adaptations to parasitism. Genomics and transcriptomics are still in their infancy in F. hepatica, with very scarce information available from the invasive newly excysted juveniles (NEJ). Here we provide an initial glimpse to the transcriptomics of the NEJ, the first stage to interact with the mammalian host.
  
  
  RESULTS
  We catalogued more than 500 clusters generated from the analysis of F. hepatica juvenile expressed sequence tags (EST), several of them not detected in the adult stage. A set of putative F. hepatica specific transcripts, and a group of sequences conserved exclusively in flatworms were identified. These novel sequences along with a set of parasite transcripts absent in the host genomes are putative new targets for future anti-parasitic drugs or vaccine development. Comparisons of the F. hepatica sequences with other metazoans genomes or EST databases were consistent with the basal positioning of flatworms in the bilaterian phylogeny. Notably, GC content, codon usage and amino acid frequencies are remarkably different in Schistosomes to F. hepatica and other trematodes. Functional annotation of predicted proteins showed a general representation of diverse biological functions. Besides proteases and antioxidant enzymes expected to participate in the early interaction with the host, various proteins involved in gene expression, protein synthesis, cell signaling and mitochondrial enzymes were identified. Differential expression of secreted protease gene family members between juvenile and adult stages may respond to different needs during host colonization.
  
  
  CONCLUSIONS
  The knowledge of the genes expressed by the invasive stage of Fasciola hepatica is a starting point to unravel key aspects of this parasite's biology. The integration of the emerging transcriptomics, and proteomics data and the advent of functional genomics tools in this organism are positioning F. hepatica as an interesting model for trematode biology.
authors.affiliation: Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, UFRGS, Porto Alegre, Brazil.
authors.forename: Martín
authors.initials: M
authors.lastname: Cancela
authors.forename: Natalia
authors.initials: N
authors.lastname: Ruétalo
authors.forename: Nicolás
authors.initials: N
authors.lastname: Dell'Oca
authors.forename: Edileuza
authors.initials: E
authors.lastname: da Silva
authors.forename: Pablo
authors.initials: P
authors.lastname: Smircich
authors.forename: Gabriel
authors.initials: G
authors.lastname: Rinaldi
authors.forename: Leda
authors.initials: L
authors.lastname: Roche
authors.forename: Carlos
authors.initials: C
authors.lastname: Carmona
authors.forename: Fernando
authors.initials: F
authors.lastname: Alvarez-Valín
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Zaha
authors.forename: José F
authors.initials: JF
authors.lastname: Tort
mesh_terms: D000818:Animals; D020224:Expressed Sequence Tags; D005210:Fasciola hepatica / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D006790:Host-Parasite Interactions