title: [De-novo generation of vascularized tissue using different configurations of vascular pedicles in perforated and closed chambers].
abstract: Growing three-dimensional tissue within a chamber requires vigorous angiogenesis initiated by, for example, an arteriovenous fistula or a ligated vascular pedicle. Growth may also be enhanced by contact with the external environment. In this study tissue growth in a rat model, vascularized via an arteriovenous loop (AV Loop) or ligated pedicle, was compared in chambers that were either closed or perforated. Chambers were harvested at 4 weeks and tissue volume and histology compared. In perforated chambers, more tissue were generated using the ligated pedicle (0.75 ml+/-0.04) than the AV Loop (0.59 ml+/-0.01). Perforated chambers generated larger volumes of tissue than closed chambers because they encouraged tissue ingrowth through the perforations. Both vessel configurations supported tissue growth but, interestingly, the ligated pedicle resulted in significantly more tissue in the perforated chambers.
authors.affiliation: Klinik für Plastische-, Hand-, Rekonstruktive- und Verbrennungschirurgie, BG-Unfallklinik, Eberhard-Karls Universität Tübingen, Tübingen, Deutschland. drdolderer@hotmail.com
authors.forename: Jürgen H
authors.initials: JH
authors.lastname: Dolderer
authors.forename: Andreas
authors.initials: A
authors.lastname: Kehrer
authors.forename: Stefan M
authors.initials: SM
authors.lastname: Schiller
authors.forename: Ulrich H
authors.initials: UH
authors.lastname: Schröder
authors.forename: Konrad
authors.initials: K
authors.lastname: Kohler
authors.forename: Hans-Eberhard
authors.initials: HE
authors.lastname: Schaller
authors.forename: Dorothea
authors.initials: D
authors.lastname: Siegel-Axel
mesh_terms: D000818:Animals; D001166:Arteriovenous Shunt, Surgical; D015194:Diffusion Chambers, Culture; D005263:Femoral Artery; D005268:Femoral Vein; D008833:Microcirculation / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013524:Surgical Flaps / Q000098:blood supply*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds