title: Isolation and characterization of Indian isolates of camel pox virus.
abstract: In this study, we isolated and identified three camel pox viruses (CMLV) from two outbreaks of camel pox infection in camels associated with eruptions on cheeks, nostrils, limbs, scrotum, and sheath that occurred at different places of Bikaner district, Rajasthan (India). The scab specimens collected were subjected for virus isolation in Vero cell culture, and the isolated viruses were characterized by employing polymerase chain reaction (PCR) and sequencing. The causative agent was identified as CMLV, based on A-type inclusion, B5R and C18L genes-specific PCRs and partial sequencing of these genes, which clearly confirmed that the outbreaks were caused by CMLV and identity of CMLV isolates. Further, phylogenetic analysis of partial C18L gene sequences have showed that Indian CMLV are clustered together with other reported isolates/strains.
authors.affiliation: Pox Virus Diseases Laboratory, Division of Virology, Indian Veterinary Research Institute, Mukteswar, 263138, Nainital, Uttarakhand, India.
authors.forename: V
authors.initials: V
authors.lastname: Bhanuprakash
authors.forename: V
authors.initials: V
authors.lastname: Balamurugan
authors.forename: M
authors.initials: M
authors.lastname: Hosamani
authors.forename: G
authors.initials: G
authors.lastname: Venkatesan
authors.forename: Bina
authors.initials: B
authors.lastname: Chauhan
authors.forename: V A
authors.initials: VA
authors.lastname: Srinivasan
authors.forename: R S
authors.initials: RS
authors.lastname: Chauhan
authors.forename: K M L
authors.initials: KM
authors.lastname: Pathak
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Singh
mesh_terms: D000818:Animals; D001483:Base Sequence; D002162:Camelus / Q000821:virology*; D002522:Chlorocebus aethiops; D016000:Cluster Analysis; D004196:Disease Outbreaks / Q000662:veterinary*; D007194:India / Q000453:epidemiology; D008957:Models, Genetic; D008969:Molecular Sequence Data; D018155:Orthopoxvirus / Q000235:genetics*; D010802:Phylogeny; D011213:Poxviridae Infections / Q000453:epidemiology* / Q000662:veterinary*; D017422:Sequence Analysis, DNA; D014709:Vero Cells