title: CMTM5-v1, a four-transmembrane protein, presents a secreted form released via a vesicle-mediated secretory pathway.
abstract: The CKLF-like MARVEL transmembrane domain-containing family (CMTM) is a novel family of proteins linking classical chemokines and the transmembrane 4 superfamily (TM4SF). Our earlier studies indicated several CMTM members (such as CKLF1 and CMTM2) have a secreted form. This is the first report of the secreted form of CMTM5-v1, the major RNA splicing form of CMTM5, which is produced as small vesicles (100 nm diameter) and floats at a peak density of 1.19 g/ml on continuous sucrose gradients. CMTM5-v1 has no obvious co-localization with CD63 or Golgi complex. In addition, brefeldin A but not wortmannin can inhibit the secretion of CMTM5-v1. Our results suggest that CMTM5-v1 might be secreted via a different vesicle-mediated secretory pathway, which will be helpful for the studies of vesicle-mediated secretion and MARVEL domain-containing proteins.
authors.affiliation: Center for Human Disease Genomics, Department of Immunology, School of Basic Medical Sciences, Health Science Center, Peking University, 38 Xueyuan Road, Beijing 100191, China.
authors.forename: Henan
authors.initials: H
authors.lastname: Li
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Guo
authors.forename: Luning
authors.initials: L
authors.lastname: Shao
authors.forename: Markus
authors.initials: M
authors.lastname: Plate
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Mo
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Wenling
authors.initials: W
authors.lastname: Han
mesh_terms: D000730:Androstadienes / Q000494:pharmacology; D015703:Antigens, CD / Q000378:metabolism; D020126:Brefeldin A / Q000494:pharmacology; D018925:Chemokines / Q000737:chemistry / Q000378:metabolism*; D006056:Golgi Apparatus; D006801:Humans; D062792:MARVEL Domain-Containing Proteins; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism; D012326:RNA Splicing; D055571:Secretory Pathway; D022142:Secretory Vesicles / Q000378:metabolism*; D060149:Tetraspanin 30; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000378:metabolism*; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D015703:Antigens, CD; C554586:CD63 protein, human; C525020:CMTM5 protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; D025521:Tumor Suppressor Proteins; D020126:Brefeldin A; D000077191:Wortmannin