Production of beta-carotene-enriched rice bran using solid-state fermentation of Rhodotorula glutinis.
This work was aimed at utilizing rice bran as a substrate for beta-carotene production by Rhodotorula glutinis DM 28 under optimized conditions of solid-state fermentation. The biomass and beta-carotene content of Rhodotorula glutinis DM 28 grown on rice bran as a sole substrate under solid-state fermentation were 54 g/kg rice bran and 1.65 mg/kg rice bran, respectively. Its biomass and beta-carotene content, however, could be improved by 60% and 30%, respectively, using the Central Composite Design for the optimization of its cultivation conditions. The optimized conditions obtained were a pH of 5, a moisture content of 70% (w/w), and a carbon-to-nitrogen ratio of 4. Under these conditions, rice bran containing R. glutinis DM 28 had nutritional values of beta-carotene, protein, and fat higher than those of rice bran alone. Yeast-grown rice bran could be suitable, therefore, to use as a beta-carotene-enriched supplement in animal feeds.
Roadjanakamolson
M
M
Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangmod, Thungkru, Bangkok 10140, Thailand.
Suntornsuk
W
W
D000821:Animal Feed / Q000382:microbiology*; D018533:Biomass; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009753:Nutritive Value; D012275:Oryza / Q000378:metabolism / Q000382:microbiology*; D012248:Rhodotorula / Q000254:growth & development / Q000378:metabolism*; D019207:beta Carotene / Q000096:biosynthesis*
D019207:beta Carotene