title: Diatrypasimilis australiensis, a novel xylarialean fungus from mangrove.
abstract: A marine xylarialean fungus, isolated from roots of Rhizophora (mangrove) in Australia, displays morphology of eight ellipsoidal dark brown ascospores in a cylindrical ascus having a refractive apical apparatus. Each ascospore has a longitudinal germ slit. The fungus grew very slowly and produced dark brown water-soluble pigment(s) on various media. It developed unique, column-shaped, indeterminate synnemata on which needle-shaped conidia were produced. The sexual stage of this fungus was not observed under the laboratory conditions tested. Molecular phylogenetic analyses of the combined sequences of nuclear ribosomal RNA genes and their internal transcribed spacers placed it at a basal position in the clade of Diatrypaceae of the Xylariales with comparatively high statistical support. However the morphological features and phylogenetic position of this organism do not closely resemble any known fungal taxa. Therefore this fungus is proposed to be a representative of a novel taxon and described as Diatrypasimilis australiensis gen. et sp. nov.
authors.affiliation: Mycology and Botany Program, American Type Culture Collection, Manassas, Virginia 20110, USA.
authors.forename: David B
authors.initials: DB
authors.lastname: Chalkley
authors.forename: Sung-Oui
authors.initials: SO
authors.lastname: Suh
authors.forename: Brigitte
authors.initials: B
authors.lastname: Volkmann-Kohlmeyer
authors.forename: Jan
authors.initials: J
authors.lastname: Kohlmeyer
authors.forename: Jianlong J
authors.initials: JJ
authors.lastname: Zhou
mesh_terms: D000165:Acridine Orange; D001315:Australia; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D043982:Rhizophoraceae; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013098:Xylariales / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; D000165:Acridine Orange