[Expression of high mobility group protein-B1 in mice with hyperoxia-induced bronchopulmonary dysplasia].
OBJECTIVE
To study the effect of hyperoxia exposure on high mobility group protein-B1 (HMGB1) expression in neonatal mice and the role of HMGB1 in the pathogenesis of bronchopulmonary dysplasia (BPD).


METHODS
C57BL/6 mice were randomly exposed to 60% O2 or air 1 day after birth. BPD was induced by 60% O2 exposure. The pulmonary tissue samples were harvested 3, 7 and 14 days after exposure. The pathologic changes of pulmonary tissues were observed by hematoxylin and eosin staining, Masson staining and radical alveolar count. The expression of HMGB1 protein in lungs was detected by immunofluorescence. The expression of HMGB1 mRNA was detected by real-time fluorescent quantitative PCR.


RESULTS
In the BPD group, the lungs developed decreased alceolar septation, swollen alveolar epithelium, stroma edema, interstitial fibrosis and developmental lag when compared with the control group. These changes became more obvious with more prolonged hyperoxia exposure. The expression of HMGB1 protein and mRNA 7 and 14 days after exposure increased significantly in the BPD group compared with that in the control group.


CONCLUSIONS
Hyperoxia exposure results in an increase in lung HMGB1 expression. The increased HMGB1 expression may be associated with the development of BPD.
Feng
Jie
J
Department of Neonatology, Affiliated Hospital, Chongqing Medical University, Chongqing 400014, China.
Deng
Chun
C
Yu
Jia-Lin
JL
Guo
Chun-Bao
CB
Zhao
Qian-Qian
QQ
D000818:Animals; D001997:Bronchopulmonary Dysplasia / Q000209:etiology*; D024243:HMGB1 Protein / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D006801:Humans; D018496:Hyperoxia / Q000150:complications*; D007231:Infant, Newborn; D008168:Lung / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis
D024243:HMGB1 Protein; D012333:RNA, Messenger