Determination of topiramate in human plasma by capillary electrophoresis with indirect UV detection.
A rapid capillary zone electrophoresis method with indirect UV detection for the determination of topiramate in human plasma was developed and validated. The analyses were carried out with a background electrolyte composed of 10mM sulfamethoxazole as chromophore in phosphate buffer (25 mM, pH 12.0); gabapentin was selected as the internal standard. Application of a voltage of +15 kV led to an analysis time shorter than 5 min; indirect UV detection was operated at 256 nm. Isolation of topiramate from plasma was accomplished by a carefully implemented solid-phase extraction procedure on C18 cartridges. The method provided a linear response over the concentration range of 2-60 microg of topiramate per mL of plasma. The limit of detection (LOD) was 0.8 microg mL(-1) and the limit of quantitation (LOQ) was 2.0 microg mL(-1). Precision, expressed as relative standard deviation, was always lower than 7.3%, extraction yields were always greater than 92%. The results obtained analysing plasma samples from epileptic patients undergoing therapy with topiramate were satisfactory in terms of precision and selectivity.
Mandrioli
Roberto
R
Laboratory of Pharmaco-Toxicological Analysis, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, I-40126 Bologna, Italy.
Musenga
Alessandro
A
Kenndler
Ernst
E
De Donno
Matteo
M
Amore
Mario
M
Raggi
Maria Augusta
MA
D002626:Chemistry, Pharmaceutical / Q000379:methods* / Q000592:standards; D019075:Electrophoresis, Capillary / Q000379:methods; D005632:Fructose / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D057230:Limit of Detection; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D000077236:Topiramate
D000077236:Topiramate; D005632:Fructose