[Development of a colloidal gold immuno-chromatography assay to detect methamphetamine].
OBJECTIVE
A rapid detector method for non-professional staff detecting methamphetamine in scene, no instrument, sensitivity and specificity, would be set up.


METHODS
Bagg albino C mice immunized with methamphetamine-modified bovine serum albumin were utilized for monoclonal antibody generation by the method of limited dilution technology. The detection of antibody typing kit was used for analysis the antibody subtype. Physical method was used for purified antibody labeled with colloidal gold. Colloidal gold test strip was composed and detected sensitivity and specificity.


RESULTS
Seven special monoclonal antibodies were screened by limited dilution technology, among which valence of purified R6 antibody reached 10(8) via ciELISA, and belonged to IgG1 by the detection of antibody typing kit. Monoclonal antibody against methamphetamine labeling with colloidal gold particles was 5 microg/ml. The sensitivity of colloidal gold immuno-chromatography assay (GICA) for methamphetamine was 500 ng/ml. The cross-reactivity of the GICA detection card to eight analogs did not appear.


CONCLUSIONS
GICA with high sensitivity, as a screening tool, may effectively contribute to Public Security Officers to detect narcotics in scene.
Xiong
Yonghua
Y
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Chen
Xuelan
X
Chen
Yuan
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000697:Central Nervous System Stimulants / Q000032:analysis*; D002845:Chromatography / Q000379:methods*; D017979:Gold Colloid; D007118:Immunoassay / Q000379:methods; D008694:Methamphetamine / Q000032:analysis*; D051379:Mice
D000911:Antibodies, Monoclonal; D000697:Central Nervous System Stimulants; D017979:Gold Colloid; D008694:Methamphetamine