Enzyme activity in the aestivating green-striped burrowing frog (Cyclorana alboguttata).
Green-striped burrowing frogs (Cyclorana alboguttata) can depress their resting metabolism by more than 80% during aestivation. Previous studies have shown that this species is able to withstand long periods of immobilisation during aestivation while apparently maintaining whole muscle mass and contractile performance. The aim of this study was to determine the effect of prolonged aestivation on the levels of metabolic enzymes (CCO, LDH and CS) in functionally distinct skeletal muscles (cruralis, gastrocnemius, sartorius, iliofibularis and rectus abdominus) and liver of C. alboguttata. CS activity was significantly reduced in all tissues except for the cruralis, gastrocnemius and the liver. LDH activity was significantly reduced in the sartorius and rectus abdominus, but remained at control (active) levels in the other tissues. CCO activity was significantly reduced in the gastrocnemius and rectus abdominus, and unchanged in the remaining tissues. Muscle protein was significantly reduced in the sartorius and iliofibularis during aestivation, and unchanged in the remaining muscles. The results suggest that the energy pathways involved in the production and consumption of ATP are remodelled during prolonged aestivation but selective. Remodelling and subsequent down-regulation of metabolic activity seem to target the smaller non-jumping muscles, while the jumping muscles retain enzyme activities at control levels during aestivation. These results suggest a mechanism by which aestivating C. alboguttata are able to maintain metabolic depression while ensuring that the functional capacity of critical muscles is not compromised upon emergence from aestivation.
Mantle
Beth L
BL
School of Biological Sciences, The University of Queensland, St Lucia 4072, Australia.
Guderley
Helga
H
Hudson
Nicholas J
NJ
Franklin
Craig E
CE
D029845:Amphibian Proteins / Q000378:metabolism*; D000818:Animals; D001001:Anura / Q000378:metabolism*; D049628:Body Size; D002950:Citrate (si)-Synthase / Q000378:metabolism; D015536:Down-Regulation; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism; D004956:Estivation / Q000502:physiology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000378:metabolism; D009928:Organ Specificity; D011793:Queensland; D011897:Random Allocation; D013997:Time Factors; D014867:Water / Q000032:analysis
D029845:Amphibian Proteins; D014867:Water; D007770:L-Lactate Dehydrogenase; D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase