title: Severely damaged kidneys possess multipotent renoprotective stem cells.
abstract: OBJECTIVE
  Tissue-specific stem cells are a promising target for kidney regeneration, because it has been shown that they play a primary role in kidney repair. Several methods have been developed for the isolation of stem/progenitor cells from healthy kidneys but the existence of these cells in chronically damaged kidneys has not been noticed so far.
  
  
  METHODS
  A mouse model of chronic kidney failure was developed by ligation of the left ureter for 5 months, and then isolation of stem cells from this tissue as well as normal kidneys was attempted.
  
  
  RESULTS
  We found that multipotent stem cells could be isolated from both types of tissue. In addition, the cells isolated from damaged kidneys showed potential for homing to the site of injury and a renoprotective effect in an animal model of cisplatin-induced nephropathy.
  
  
  CONCLUSIONS
  These results show that multipotent renoprotective stem cells exist in severely damaged kidneys, which could be a target for designing new therapies.
authors.affiliation: Department of Stem Cell and Tissue Engineering and Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Yousof
authors.initials: Y
authors.lastname: Gheisari
authors.forename: Seyed Mahdi
authors.initials: SM
authors.lastname: Nassiri
authors.forename: Ehsan
authors.initials: E
authors.lastname: Arefian
authors.forename: Naser
authors.initials: N
authors.lastname: Ahmadbeigi
authors.forename: Kayhan
authors.initials: K
authors.lastname: Azadmanesh
authors.forename: Mansour
authors.initials: M
authors.lastname: Jamali
authors.forename: Issa
authors.initials: I
authors.lastname: Jahanzad
authors.forename: Sirous
authors.initials: S
authors.lastname: Zeinali
authors.forename: Mohammad
authors.initials: M
authors.lastname: Vasei
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins