Modulation of aged murine T lymphocytes in vivo by DPV576-C, a nanodiamond- and nanoplatinum-coated material.
BACKGROUND
Nanotechnology is rapidly emerging in biomedical applications, including cancer therapy. Here, a mixture of ultra dispersed nanodiamond and nanoplatinum was coated onto fabrics in the form of a cloth (DPV576-C). The role of DPV576-C in modulating T lymphocytes of aged mice was examined.


METHODS
C57BL/6 mice were treated with DPV576-C as a lining in a mouse house for 1 month. Splenic cells were analyzed for CD4(+) and CD8(+) T-cells and NK activity using flow cytometry.


RESULTS
DPV576-C-treated aged mice showed an: (1) increase in the percentages of CD4(+) and CD8(+) T-cells and their activation markers, CD25 and CD69, over untreated aged mice; (2) enhancement of NK activity; and (3) absence of adverse side effects as determined histopathologically.


CONCLUSIONS
The enhancement of lymphocytes by DPV576-C may be useful for patients suffering from immune dysfunction.
Ghoneum
Mamdooh
M
Charles Drew University of Medicine and Science, Department of Otolaryngology, Los Angeles, CA 90059, USA. mghoneum@ucla.edu
Ghoneum
Alia
A
Tolentino
Lucilene
L
Gimzewski
James
J
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D016922:Cellular Senescence / Q000276:immunology*; D020099:Coated Materials, Biocompatible; D018130:Diamond; D005260:Female; D005434:Flow Cytometry; D006799:Housing, Animal; D007154:Immune System Diseases / Q000276:immunology / Q000628:therapy*; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D057139:Nanofibers; D010984:Platinum; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D020099:Coated Materials, Biocompatible; D018130:Diamond; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D010984:Platinum; D000099192:CD69 Antigens; C508018:Il2ra protein, mouse