Molecular analysis of a UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) gene from purple potato (Solanum tuberosum).
In anthocyanin biosynthesis, UDP-glucose: anthocyanidin 3-O-glucosyltransferase (UFGT) catalyzes the transfer of the glucosyl moiety from UDP-glucose to the 3-hydroxyl group of anthocyanidins, producing the first stable anthocyanins. The full-length cDNA of UFGT (designated as StUFGT) was isolated and characterized from Solanum tuberosum. The full-length cDNA of StUFGT was 1536 bp containing a 1344 bp open reading frame (ORF) encoding 448 amino acids with a calculated molecular mass of 49.9 kDa and an isoelectric point of 5.62. Comparative and bioinformatic analyses revealed that StUFGT has extensive homology with UFGTs from other plant species. Phylogenetic analysis indicates that StUFGT belongs to the plant UFGT cluster. StUFGT was found to be expressed in roots, stems, leafstalks and leaves. Expression profiling analysis revealed that StUFGT expression was induced correspondingly by exogenous elicitors including gibberellic acid and sucrose, suggesting that UFGT might play a regulatory role in anthocyanin biosynthesis in Solanum tuberosum at the transcriptional level.
Hu
Chaoyang
C
Key Laboratory of Applied Marine Biotechnology (Ningbo University), Minister of Education, Faculty of Life Science and Biotechnology, Ningbo University, 315211, Ningbo, Zhejiang Province, People's Republic of China. spiritsun@21cn.com
Gong
Yifu
Y
Jin
Si
S
Zhu
Qing
Q
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005964:Glucosyltransferases / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000201:enzymology* / Q000235:genetics*
D018076:DNA, Complementary; D005964:Glucosyltransferases; C023249:flavonol 3-O-glucosyltransferase