title: High-resolution separation of tubulin monomers on polyacrylamide minigels.
abstract: High-resolution separation of alpha- and beta-tubulin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on minigels can be performed rapidly using simple modifications of the standard Laemmli procedure. Separation of the subunits can be observed even in high-protein loads (up to 40microg of protein).
authors.affiliation: Department of Chemistry, Binghamton University, NY 13902, USA.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Banerjee
authors.forename: Frank A
authors.initials: FA
authors.lastname: Bovenzi
authors.forename: Susan L
authors.initials: SL
authors.lastname: Bane
mesh_terms: D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000191:economics / Q000379:methods*; D021122:Protein Subunits / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D014404:Tubulin / Q000302:isolation & purification*
chemical_list: D021122:Protein Subunits; D014404:Tubulin; D012967:Sodium Dodecyl Sulfate