Identification of differentially expressed genes associated with osteochondrosis in standardbred horses using RNA arbitrarily primed PCR.
The aim of this study was to investigate genes for differential expression in cartilage of foals predisposed to osteochondrosis (OC). Tissue was sampled from the cranial part of the distal intermediate ridge of the tibia in the tarso-crural joint. Foals were considered predisposed to OC when parents had OC at the distal intermediate ridge of the tibia. RNA was isolated and subjected to arbitrarily primed PCR (RAP-PCR) followed by fingerprinting to screen for differentially expressed genes. By verification of results from the RAP-PCR fingerprint screening using real-time RT-PCR, we identified two genes not previously correlated with OC as differentially expressed. The two genes, which were identical to TLK2 and an equine EST, are good targets for future research on OC.
Austbø
Lars
L
Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, Oslo, Norway. lars.austbo@veths.no
Røed
Knut H
KH
Dolvik
Nils I
NI
Skretting
Grethe
G
D000818:Animals; D002356:Cartilage / Q000378:metabolism; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation / Q000502:physiology*; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006734:Horse Diseases / Q000235:genetics*; D006736:Horses; D055034:Osteochondrosis / Q000235:genetics / Q000662:veterinary*