title: Low-dose radiation-induced hormetic effect on hematopoietic reconstitution.
abstract: OBJECTIVE
  To investigate the effects on hematopoietic reconstitution of recipient mice receiving bone marrow cells (BMC) of donor pre-exposed to low-dose radiation (LDR).
  
  
  METHODS
  BMC were irradiated in vitro at various low-dose X-rays radiation. Tritiated thymidine ((3)H-TdR) incorporation was adopted to measure the proliferation of the BMC in vitro, and then the optimal radiation doses were selected for further study in vivo. Irradiated recipient mice (7.5 Gy) were infused by BMC exposed to 6 and 8 cGy. The counts of recipients' peripheral blood cells (PBC) and bone marrow mononuclear cells (BMMNC) were monitored at the 5th, 10th, 15th, and 30th day after BMC infusion. The colony-forming units in the recipient spleens (CFU-S) were calculated at the 10th day after infusion.
  
  
  RESULTS
  Exposed to 6 and 8 cGy, BMC demonstrated significant proliferative activities in vitro. When the recipient mice receiving BMC pre-exposed to low dose radiation (6 and 8 cGy), the counts of white blood cell (WBC), BMMNC and CFU-S were consistently higher than those in control.
  
  
  CONCLUSIONS
  BMC of donor irradiated by LDR in vitro may facilitate the hematopoietic reconstitution of the recipient mice.
authors.affiliation: The Second Affiliated Hospital of Soochow University, Jiangsu, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhang
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.forename: Yongyou
authors.initials: Y
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Wang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Huo
authors.forename: Yusong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Mingzhi
authors.initials: M
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Ning
authors.forename: Jiagui
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D001773:Blood Cells / Q000166:cytology / Q000528:radiation effects; D001854:Bone Marrow Cells / Q000166:cytology / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D049109:Cell Proliferation / Q000528:radiation effects; D004781:Environmental Exposure; D006410:Hematopoiesis / Q000502:physiology* / Q000528:radiation effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D011829:Radiation Dosage; D013997:Time Factors