Changes in oxidative enzyme activity during interspecific mycelial interactions involving the white-rot fungus Trametes versicolor.
Interspecific fungal antagonism leads to biochemical changes in competing mycelia, including up-regulation of oxidative enzymes. Laccase, manganese peroxidase (MnP), manganese-repressed peroxidase (MRP) and lignin peroxidase (LiP) gene expression and enzyme activity were compared during agar interactions between Trametes versicolor and five other wood decay fungi resulting in a range of interaction outcomes from deadlock to replacement of one fungus by another. Increased laccase and Mn-oxidising activities were detected at all interaction zones, but there were few changes in activity in regions away from the interaction zone in T. versicolor mycelia compared to self-pairings. Whilst no LiP activity was detected in any pairing, low level LiP gene expression was detected. MnP activity was detected but not expression of MnP genes; instead, MRP could explain the observed activity. No relationship was found between extent of enzyme activity increase and interaction outcome. Similarities between patterns of gene expression and enzyme activity are discussed.
Hiscox
Jennifer
J
BIOSI 2, Cardiff University, King Edward VII Avenue, Cardiff, UK. evansja7@cf.ac.uk
Baldrian
Petr
P
Rogers
Hilary J
HJ
Boddy
Lynne
L
D001487:Basidiomycota / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D042845:Laccase / Q000235:genetics / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism; D056265:Microbial Interactions; D025282:Mycelium / Q000502:physiology*; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D055454:Trametes / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation; D014934:Wood / Q000382:microbiology; D013098:Xylariales / Q000201:enzymology / Q000235:genetics / Q000502:physiology*
D008345:Manganese; D042845:Laccase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase