Improvement of coenzyme Q(10) production by increasing the NADH/NAD(+) ratio in Agrobacterium tumefaciens.
Previously screened CoQ(10)-overproducing Agrobacterium tumefaciens A603-35 showed a relatively high NADH/NAD(+) ratio (1.1), as compared to parental strain C58 (0.2) when we increased the expression levels of NADH-generating enzymes. Also, the intracellular NADH/NAD(+) ratio showed a positive correlation with the CoQ(10) content in A603-35. Overexpression of glyceraldehyde 3-phosphate dehydrogenase in A603-35 shifted the NADH/NAD(+) ratio at 48 h from 0.8 to 1.2, and thus the CoQ(10) content in flask culture increased from 2.16 to 3.63 mg/g DCW. Due to the addition of hydroxybutyrate to the culture media, the intracellular NADH/NAD(+) ratio in A603-35-gapA shifted from 1.2 to 1.4, which led to an increase CoQ(10) content (5.27 mg/g DCW).
Koo
Bong-Seong
BS
BioNgene Co., Ltd., Seoul, Republic of Korea.
Gong
Yun-Jung
YJ
Kim
Sang-Yong
SY
Kim
Chan-Wha
CW
Lee
Hyeon-Cheol
HC
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005986:Glyceraldehyde 3-Phosphate / Q000235:genetics / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D009243:NAD / Q000235:genetics / Q000378:metabolism*; D014451:Ubiquinone / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
D003470:Culture Media; D009243:NAD; D014451:Ubiquinone; D005986:Glyceraldehyde 3-Phosphate; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases