title: Assimilation of metal ions bound to porphyrins or porphyrin-peptides by vibrio vulnificus, a human pathogen inhabiting estuarine and marine environments.
abstract: Vibrio vulnificus, a ubiquitous microorganism in aquatic environments, causes serious septicemia to the immunocompromised host. In addition to protoheme, this species can utilize Fe-TCPP [ferric tetrakis (4-carboxyphenyl) porphine] as an iron source. In the present study, heme c bound covalently to the protein in cytochrome c, as well as the Fe-TCPP complex formed with a nanopeptide with a high affinity, was found to be useful iron sources for V. vulnificus. This bacterium was also revealed to use Zn-TCPP as a single zinc source. However, other metalloporphyrins such as Mn-TCPP and Pt-TCPP delayed the bacterial growth in the broth containing Fe-TCPP, suggesting interference in the iron assimilation. These results indicate that V. vulnificus may acquire metal ions from both free and peptide-bound metalloporphyrins.
authors.affiliation: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Tushima-Naka, Okayama 700-8530, Japan. miyoshi@pharm.okayama-u.ac.jp
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Miyoshi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sasaki
authors.forename: Nahoko
authors.initials: N
authors.lastname: Kaku
authors.forename: Takaharu
authors.initials: T
authors.lastname: Inoue
authors.forename: Natsuki
authors.initials: N
authors.lastname: Uozumi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Maehara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakao
mesh_terms: D045304:Cytochromes c / Q000378:metabolism; D019015:Geologic Sediments; D008665:Metalloporphyrins / Q000378:metabolism*; D041261:Vibrio vulnificus / Q000254:growth & development / Q000378:metabolism*
chemical_list: D008665:Metalloporphyrins; C473248:iron 5,10,15,20-tetrakis-4-carboxyphenylporphyrin; C067542:zinc(II) tetrakis(4-carboxyphenyl)porphine; D045304:Cytochromes c