title: Accumulation of 1-deoxynojirimycin in silkworm, Bombyx mori L.
abstract: 1-deoxynojirimycin (1-DNJ) contents in the silkworm, Bombyx mori, at different developmental stages and tissues were investigated by using reverse-phase high-performance liquid chromatography. The 1-DNJ contents of silkworm larvae change significantly with their developmental stages. The male larvae showed higher accumulation efficiency of 1-DNJ than the females and also a significant variation was observed among the silkworm strains. The present results show that tissue distribution of 1-DNJ was significantly higher in blood, digestive juice, and alimentary canal, but no 1-DNJ was observed in the silkgland. Moreover, 1-DNJ was not found in silkworms fed with artificial diet that does not contain mulberry leaf powder. This proves that silkworms obtain 1-DNJ from mulberry leaves; they could not synthesize 1-DNJ by themselves. The accumulation and excretion of 1-DNJ change periodically during the larval stage. There was no 1-DNJ in the newly-hatched larvae and 1-DNJ was mainly accumulated during the early and middle stages of every instar, while excreted at later stages of larval development. Further, it is possible to extract 1-DNJ from the larval feces and it is optimal to develop the 1-DNJ related products for diabetic auxiliary therapy.
authors.affiliation: Key Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University, Taian 271018, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yin
authors.forename: Xin-qin
authors.initials: XQ
authors.lastname: Shi
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Ye
authors.forename: Zu-an
authors.initials: ZA
authors.lastname: Duan
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Zhou
authors.forename: Wei-zheng
authors.initials: WZ
authors.lastname: Cui
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Wu
mesh_terms: D017485:1-Deoxynojirimycin / Q000737:chemistry / Q000493:pharmacokinetics*; D000821:Animal Feed; D000818:Animals; D012831:Bombyx / Q000196:embryology / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D007814:Larva / Q000378:metabolism; D008297:Male; D031625:Morus; D018515:Plant Leaves / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D017485:1-Deoxynojirimycin