title: The effect of nanotubular titanium surfaces on osteoblast differentiation.
abstract: The biological response of fetal rat calvarial cells on a TiO2 nanotubular surface (Ti-NT) was evaluated by cell viability assay, alkaline phosphatase (ALP) activity and reverse transcription polymerase chain reaction (RT-PCR) analysis. The cell viability assay showed no significant difference between the Ti-NT and smooth titanium surfaces (Ti-S). Ti-NT had better cellular responses with regard to the ALP activity and bone-associated markers, such as bone sialoprotein and osteocalcin mRNA than Ti-S. These results suggest that Ti-NT stimulate the differentiation into osteoblasts of fetal rat calvarial cells, potentially contributing to rapid osseointegration.
authors.affiliation: Dental Research Institute, School of Dentistry, 2nd Stage of Brain Korea 21 Project, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Kim
authors.forename: Kwan-Pyo
authors.initials: KP
authors.lastname: Cho
authors.forename: Young-Suk
authors.initials: YS
authors.lastname: Chung
authors.forename: Ok-Su
authors.initials: OS
authors.lastname: Kim
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Chung
authors.forename: Kyung-Ku
authors.initials: KK
authors.lastname: Lee
authors.forename: Doh-Jae
authors.initials: DJ
authors.lastname: Lee
authors.forename: Kwang-Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D010316:Particle Size; D051381:Rats; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D046911:Macromolecular Substances; C009495:titanium dioxide; D014025:Titanium