title: Trans-differentiation of iris pigmented epithelial cells of Euphlyctis cyanophlyctis tadpoles into lens in vitro.
abstract: Meshed pigmented iris epithelium along with neural retina of tadpoles of the frog Euphlyctis cyanophlyctis were found to undergo dedifferentiation and subsequently transdifferentiate into lens in culture medium. During lag period, depigmentation (dedifferentiation) occurred in many cells. When culture became confluent 3-4 weeks after seeding tiny lens like structures differentiated from foci of cultured pigmented iris epithelium cells. The percentage of lens formation was higher in vitamin A treated cases. The culture system appears to be a suitable for investigating the changes occurred during trans-differentiation of pigmented epithelial cells into lens.
authors.affiliation: Developmental Biology Laboratory, Department of Zoology, Dungar College, Bikaner 334 401, India.
authors.forename: Manshi
authors.initials: M
authors.lastname: Sharma
authors.forename: O P
authors.initials: OP
authors.lastname: Jangir
authors.forename: Sunita
authors.initials: S
authors.lastname: Jhajharia
authors.forename: Sumer
authors.initials: S
authors.lastname: Singh
authors.forename: Vijay
authors.initials: V
authors.lastname: Singh
authors.forename: Amit
authors.initials: A
authors.lastname: Nagal
mesh_terms: D000818:Animals; D054338:Cell Transdifferentiation / Q000187:drug effects; D007498:Iris / Q000166:cytology* / Q000187:drug effects; D007814:Larva / Q000166:cytology / Q000254:growth & development; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000187:drug effects; D011898:Ranidae / Q000033:anatomy & histology / Q000254:growth & development*; D046509:Tissue Culture Techniques; D014801:Vitamin A / Q000494:pharmacology
chemical_list: D014801:Vitamin A