title: [The expression of Basigin mRNA in early ischemic myocardium and non-ischemic myocardium].
abstract: OBJECTIVE
  To investigate the expression of Basigin mRNA in early ischemic myocardium (EIM) and non-ischemic myocardium (NIM) in rat.
  
  
  METHODS
  Real-time polymerase chain reaction (PCR) technique was applied for detecting Basigin mRNA and beta-actin expression in EIM and NIM at 0 min, 15 min, 30 min, and 60 min.
  
  
  RESULTS
  No significant differences of the Basigin mRNA expression in EIM and NIM between 0 min and sham operation (SO) or non-operation group (P > 0.05) were observed. Basigin mRNA expression in EIM significantly decreased in 15 min, 30 min, and 60 min group compared with that of 0 min group, respectively (P < 0.001). However, we failed to find any significant difference in NIM and NO from 0 min to 60 min. Basigin mRNA expression in EIM decreased significantly compared with that of in NIM (P < 0.05) after myocardial ischemia for 30 min.
  
  
  CONCLUSIONS
  Basigin was involved in myocardium pathophysiology process after myocardial ischemia for 30 min, indicating that Basigin may be identified as a predictor of early myocardial ischemia in forensic medicine.
authors.affiliation: Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Lin-Bo
authors.initials: LB
authors.lastname: Gao
authors.forename: Wei-Bo
authors.initials: WB
authors.lastname: Liang
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Pan
authors.forename: Yan-Yun
authors.initials: YY
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Xue
authors.forename: Tian-Yi
authors.initials: TY
authors.lastname: Chen
authors.forename: Lu-Shun
authors.initials: LS
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D051926:Basigin / Q000235:genetics / Q000378:metabolism*; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism*; D005554:Forensic Medicine; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D001798:Blood Proteins; C079432:Bsg protein, rat; D012333:RNA, Messenger; D051926:Basigin