Protective effects of silica hydride against carbon tetrachloride-induced hepatotoxicity in mice.
The protective effects of MegaHydrate silica hydride against liver damage were evaluated by its attenuation of carbon tetrachloride (CCl(4))-induced hepatotoxicity in mice. Male ICR mice were orally treated with silica hydride (104, 208 and 520 mg/kg) or silymarin (200 mg/kg) daily, with administration of CCl(4) (1 mL/kg, 20% CCl4 in olive oil) twice a week for eight weeks. The results showed that oral administration of silica hydride significantly reduced the elevated serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), triglyceride (TG), and cholesterol and the level of malondialdehyde (MDA) in the liver that were induced by CCl(4) in mice. Moreover, the silica-hydride treatment was also found to significantly increase the activities of superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH-Px), as well as increase the GSH content, in the liver. Liver histopathology also showed that silica hydride reduced the incidence of liver lesions induced by CCl(4). The results suggest that silica hydride exhibits potent hepatoprotective effects on CCl(4)-induced liver damage in mice, likely due to both the increase of antioxidant-defense system activity and the inhibition of lipid peroxidation.
Hsu
Yu-Wen
YW
Department of Applied Chemistry, Chung Shan Medical University, Taichung City, Taiwan.
Tsai
Chia-Fang
CF
Chuang
Wen-Chen
WC
Chen
Wen-Kang
WK
Ho
Yung-Chyuan
YC
Lu
Fung-Jou
FJ
D000410:Alanine Transaminase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000378:metabolism / Q000517:prevention & control*; D002784:Cholesterol / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D017640:Silicates / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
D017640:Silicates; D014280:Triglycerides; C487012:silica hydride; D008315:Malondialdehyde; D002784:Cholesterol; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase