Effect of beta carotene on lipid peroxidation and antioxidant status following renal ischemia/reperfusion injury in rat.
BACKGROUND
The production of free radicals and reactive oxygen species are important factors contributing to ischemia/reperfusion injury. Thus scavenging of the excess free radicals can be an important therapeutic approach. Beta carotene, a carotenoid pigment has a potent antioxidant property. The present study examined the effect of beta carotene administration on the level of renal content of antioxidants and lipid peroxidation following ischemia/reperfusion injury in the rat kidney.


METHODS
Male adult Wistar rats (250-300 g) were exposed to 45 min of renal ischemia followed by 4 h of reperfusion. Beta carotene (10, 30 and 100 mg kg(-1)) or vehicle was administered for 5 days prior to ischemia. Renal content of antioxidants and the level of lipid peroxidation were measured after the reperfusion period.


RESULTS
Our results showed that ischemia/reperfusion injury increased lipid peroxidation (p < 0.001) and decreased antioxidant (p < 0.001) in renal tissue. Pre-administration of beta carotene could attenuate these alterations (p < 0.05-p < 0.001), although not at all doses. Since beta carotene administration improved renal lipid peroxidation and antioxidants, it seems that beta carotene protects renal tissue against ischemia/reperfusion-induced oxidative damage.
Hosseini
F
F
Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, PO Box: 61335-189, Ahvaz, Iran. hosseinif@yahoo.com
Naseri
M K Gharib
MK
Badavi
M
M
Ghaffari
M A
MA
Shahbazian
H
H
Rashidi
I
I
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000201:enzymology / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014815:Vitamins / Q000627:therapeutic use*; D019207:beta Carotene / Q000627:therapeutic use*
D000975:Antioxidants; D014815:Vitamins; D019207:beta Carotene; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase