title: Separation of peptides and oligonucleotides using a monolithic polymer layer and pressurized planar electrophoresis and electrochromatography.
abstract: The rapid separation of mixtures of six peptides using porous polymer monolithic layers in electrophoresis and pressurized planar electrochromatography modes has been achieved. The separations in the former mode were performed on a generic hydrophobic poly(butyl methacrylate-co-ethylene dimethacrylate) layer with no ionizable functionalities and required 2 min. This layer also enabled the separation of three oligonucleotides. The separation in the pressurized planar electrochromatographic mode was carried out using a negatively charged layer prepared via cografting of 2-acrylamido-2-methyl-1-propanesulfonic acid and 2-hydroxyethyl methacrylate on top of the generic hydrophobic monolith and was completed in 1 min.
authors.affiliation: Department of Chemistry and Chemical Biology, Indiana University, Indianapolis, 402 North Blackford Street, Indianapolis, Indiana 46202, USA.
authors.forename: Scott D
authors.initials: SD
authors.lastname: Woodward
authors.forename: Iva
authors.initials: I
authors.lastname: Urbanova
authors.forename: David
authors.initials: D
authors.lastname: Nurok
authors.forename: Frantisek
authors.initials: F
authors.lastname: Svec
mesh_terms: D000399:Air Pressure; D020374:Chromatography, Micellar Electrokinetic Capillary; D004586:Electrophoresis; D009841:Oligonucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D040901:Proteomics
chemical_list: D009841:Oligonucleotides; D010455:Peptides; D011108:Polymers