title: Methyl caffeate as an alpha-glucosidase inhibitor from Solanum torvum fruits and the activity of related compounds.
abstract: In screening experiments for rat intestinal alpha-glucosidase (sucrase and maltase) inhibitors in 325 plants cultivated in Japan's southern island, of Tanegashima, marked inhibition against both sucrase and maltase was found in the extract of the fruit of Solanum torvum. Enzyme-assay guided fractionation of the extract led to the isolation of methyl caffeate (1) as a rat intestinal sucrase and maltase inhibitor. We examined 13 caffeoyl derivatives for sucrase- and maltase-inhibitory activities. The results showed that methyl caffeate (1) had a most favorable structure for both sucrase and maltase inhibition, except for a higher activity of methyl 3,4,5-trihydroxycinnamate (14) against sucrase. Its moderate inhibitory action against alpha-glucosidase provides a prospect for antidiabetic usage of S. torvum fruit.
authors.affiliation: Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Takahashi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Yoshioka
authors.forename: Eisuke
authors.initials: E
authors.lastname: Kato
authors.forename: Shigeki
authors.initials: S
authors.lastname: Katsuki
authors.forename: Osamu
authors.initials: O
authors.lastname: Iida
authors.forename: Keizo
authors.initials: K
authors.lastname: Hosokawa
authors.forename: Jun
authors.initials: J
authors.lastname: Kawabata
mesh_terms: D000818:Animals; D002109:Caffeic Acids; D057075:Enzyme Assays; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D005638:Fruit / Q000201:enzymology*; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007422:Intestines / Q000187:drug effects; D007564:Japan; D051381:Rats; D032322:Solanum / Q000201:enzymology*; D013393:Sucrase / Q000037:antagonists & inhibitors
chemical_list: D002109:Caffeic Acids; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; C042405:methyl caffeate; D013393:Sucrase