Hydrogen-rich saline protects against spinal cord injury in rats.
In the present study, we examined the mechanisms of hydrogen-rich saline, a reported therapeutic antioxidant, in the treatment of acute spinal cord contusion injury. Male Sprague-Dawley rats were used to produce a standardized model of contuses spinal cord injury (125 kdyn force). Hydrogen-rich saline was injected intraperitoneally (5 ml/kg) immediately, and at 24 and 48 h after injury. All rats were sacrificed at 72 h after spinal cord injury (SCI). Apoptotic cell death, oxidative stress, inflammation, level of Brain derived neurotrophic factor (BDNF) were evaluated. In addition, locomotor behavior was assessed using the Basso, Beattice and Bresnahan (BBB) scale. We observed that administration of hydrogen-rich saline decreased the number of apoptotic cells, suppressed oxidative stress, and improved locomotor functions. Hydrogen-rich saline increased the release of BDNF. In conclusion, hydrogen-rich saline reduced acute spinal cord contusion injury, possibly by reduction of oxidative stress and elevation of BDNF.
Chen
Chengwen
C
Department of Anesthesiology, Changzheng Hospital, Second Military Medical University, 200003, Shanghai, People's Republic of China.
Chen
Qianbo
Q
Mao
Yanfei
Y
Xu
Shengming
S
Xia
Chunyan
C
Shi
Xueyin
X
Zhang
John H
JH
Yuan
Hongbin
H
Sun
Xuejun
X
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D017209:Apoptosis; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D016923:Cell Death; D006614:Hindlimb / Q000503:physiopathology; D006859:Hydrogen / Q000627:therapeutic use*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D050050:Protein Carbonylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000627:therapeutic use*; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*
D000975:Antioxidants; D019208:Brain-Derived Neurotrophic Factor; D012965:Sodium Chloride; D008315:Malondialdehyde; D006859:Hydrogen; D009195:Peroxidase; D020169:Caspases