Effect of neurotrophin-3 genetically modified olfactory ensheathing cells transplantation on spinal cord injury.
Transplantation of olfactory ensheathing cells (OECs) has emerged as a very promising therapy for spinal cord injury (SCI). Also, local delivery of NT-3 can counteract pathological events and induce a regenerative response after SCI. Supplement of exogenetic NT-3 might be a new approach to SCI repair. In this study, we examined the therapeutic effect of rat NT-3 gene-modified OECs transplantation on SCI. Rat NT-3 gene was transfected into OECs using a retroviral system. The engineered NT-3-OECs were tested for their ability to express and secrete biologically active NT-3 in vitro. Then NT-3-OECs were implanted into contused T9 spinal cord of the adult rats. Their ability of survival and NT-3 production was examined. The effect of axon regeneration was evaluated at the morphological level and promotion of locomotor functional recovery were assessed. The result showed that genetically modified OECs were capable of surviving and producing NT-3 in vivo to significantly improve the recovery after SCI.
Ma
Yu-Hai
YH
Department of Orthopaedic, Zhejiang Provincial Corps Hospital, Jiaxing, China.
Zhang
Yong
Y
Cao
Li
L
Su
Jia-Can
JC
Wang
Zhi-Wei
ZW
Xu
A-Bing
AB
Zhang
Shao-Cheng
SC
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D017690:Cell Transplantation; D002478:Cells, Cultured; D005260:Female; D051379:Mice; D009043:Motor Activity / Q000502:physiology; D041681:NIH 3T3 Cells; D009416:Nerve Regeneration / Q000502:physiology; D009457:Neuroglia / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D020933:Neurotrophin 3 / Q000235:genetics / Q000378:metabolism*; D009833:Olfactory Pathways / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D013119:Spinal Cord Injuries / Q000473:pathology / Q000601:surgery*
D020933:Neurotrophin 3