title: Quasispecies of the D225G substitution in the hemagglutinin of pandemic influenza A(H1N1) 2009 virus from patients with severe disease in Hong Kong, China.
abstract: The D225G (aspartic acid to glycine) substitution in the hemagglutinin of H1N1 influenza virus may alter its receptor-binding specificity. Direct analysis of polymorphisms in 126 amino acids spanning the receptor-binding site in the hemagglutinin of pandemic H1N1 2009 virus from 117 clinical specimens in Hong Kong found the D225G substitution for 7 (12.5%) of 57 patients with severe disease and for 0 (0%) of 60 patients with mild disease. D225G quasispecies were identified mainly in endotracheal aspirate samples and were identified less frequently in nasopharyngeal aspirate samples from patients with severe disease. Continuous monitoring of the prevalence and tissue tropism of this variant during its circulation among humans is important.
authors.affiliation: State Key Laboratory for Emerging Infectious Diseases, University of Hong Kong, China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Chen
authors.forename: Xi
authors.initials: X
authors.lastname: Wen
authors.forename: Kelvin K W
authors.initials: KK
authors.lastname: To
authors.forename: Pui
authors.initials: P
authors.lastname: Wang
authors.forename: Herman
authors.initials: H
authors.lastname: Tse
authors.forename: Jasper F W
authors.initials: JF
authors.lastname: Chan
authors.forename: Hoi-Wah
authors.initials: HW
authors.lastname: Tsoi
authors.forename: Kitty S C
authors.initials: KS
authors.lastname: Fung
authors.forename: Cindy W S
authors.initials: CW
authors.lastname: Tse
authors.forename: Rodney A
authors.initials: RA
authors.lastname: Lee
authors.forename: Kwok-Hung
authors.initials: KH
authors.lastname: Chan
authors.forename: Kwok-Yung
authors.initials: KY
authors.lastname: Yuen
mesh_terms: D019943:Amino Acid Substitution; D004196:Disease Outbreaks; D006388:Hemagglutinins / Q000235:genetics*; D006723:Hong Kong / Q000453:epidemiology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics*; D007251:Influenza, Human / Q000453:epidemiology* / Q000821:virology*
chemical_list: D006388:Hemagglutinins