title: [Valproic acid induce the expression of MICA antigen in human lung cancer cells and promote the NK killing effects on the cells].
abstract: OBJECTIVE
  To investigate the effect of valproic acid (VPA) on MICA gene expression in human lung carcinoma cell lines: A549, SP-CA-1, NCIH446.
  
  
  METHODS
  Different concentration of VPA were applied to treat A549, SP-CA-1, NCIH446 cells respectively and the untreated cells as the control. The effect of the VPA on the cells were observed. Reverse Transcriptase PCR and Immune-Fluorescence staining were used to detect the changes of mRNA, protein level of MICA gene in the three cell lines treated with 1.50 mmol/L and 3.00 mmol/L VPA for 4 days under the concentration of VPA from 0.75 to 12.0 mmol/L. The viability of lung cancer cells after NK effect in the VPA(+) and VPA(-) treatment were evaluated with LDH method.
  
  
  RESULTS
  The inhibition effects were observed when the VPA concentration was 6.0 mmol/L above compared to control group, VPA induced the increasing expression of MICA and in turn enhanced the NK killing effect on the cells in a concentration-depended pattern.
  
  
  CONCLUSIONS
  VPA induced MICA expression, NK killing effect in the VPA(+) treatment lung cancer cells was significantly stronger than that of VPA(-) condition, which implied a new mechanism of anticancer method and could be a new approach to cancer therapy.
authors.affiliation: Department of Cardiothoracic Surgery, Chengdu Third People's Hospital, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Rui
authors.forename: Dan
authors.initials: D
authors.lastname: Long
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Chou
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Zhang
authors.forename: Xin-Jian
authors.initials: XJ
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Fang
authors.forename: Wu
authors.initials: W
authors.lastname: Yuan
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014635:Valproic Acid / Q000494:pharmacology*
chemical_list: D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; D012333:RNA, Messenger; D014635:Valproic Acid