Cellulose nanocrystal/gold nanoparticle composite as a matrix for enzyme immobilization.
A novel nanocomposite consisting of cellulose nanocrystals (CNCs) functionalized with gold nanoparticles (AuNPs) serving as an excellent support for enzyme immobilization with phenomenally high loading is presented in this work. As testing models, cyclodextrin glycosyl transferase (CGTase) and alcohol oxidase were conjugated on an activated CNC/AuNP matrix. This catalytic platform exhibits significant biocatalytic activity with excellent enzyme stability and without apparent loss of the original activity. The recovered specific activities were approximately 70% and 95% for CGTase and alcohol oxidase, respectively. This novel and inexpensive material is anticipated to extend to other enzymes, enhancing the enzyme loading and activity as well as the stability in both operation and storage.
Mahmoud
Khaled A
KA
Biotechnology Research Institute, Canada National Research Council, 6100 Royalmount Avenue, Montreal, Canada H4P 2R2.
Male
Keith B
KB
Hrapovic
Sabahudin
S
Luong
John H T
JH
D000429:Alcohol Oxidoreductases / Q000737:chemistry; D001671:Biochemistry / Q000379:methods; D002234:Carbodiimides / Q000737:chemistry; D002384:Catalysis; D002482:Cellulose / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005964:Glucosyltransferases / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010843:Pichia / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D002234:Carbodiimides; D004800:Enzymes, Immobilized; D006046:Gold; D002482:Cellulose; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase