[Valproic acid induce the expression of MICA antigen in human lung cancer cells and promote the NK killing effects on the cells].
OBJECTIVE
To investigate the effect of valproic acid (VPA) on MICA gene expression in human lung carcinoma cell lines: A549, SP-CA-1, NCIH446.


METHODS
Different concentration of VPA were applied to treat A549, SP-CA-1, NCIH446 cells respectively and the untreated cells as the control. The effect of the VPA on the cells were observed. Reverse Transcriptase PCR and Immune-Fluorescence staining were used to detect the changes of mRNA, protein level of MICA gene in the three cell lines treated with 1.50 mmol/L and 3.00 mmol/L VPA for 4 days under the concentration of VPA from 0.75 to 12.0 mmol/L. The viability of lung cancer cells after NK effect in the VPA(+) and VPA(-) treatment were evaluated with LDH method.


RESULTS
The inhibition effects were observed when the VPA concentration was 6.0 mmol/L above compared to control group, VPA induced the increasing expression of MICA and in turn enhanced the NK killing effect on the cells in a concentration-depended pattern.


CONCLUSIONS
VPA induced MICA expression, NK killing effect in the VPA(+) treatment lung cancer cells was significantly stronger than that of VPA(-) condition, which implied a new mechanism of anticancer method and could be a new approach to cancer therapy.
Rui
Jun
J
Department of Cardiothoracic Surgery, Chengdu Third People's Hospital, China.
Long
Dan
D
Wang
Wei
W
Chou
Tao
T
Zhang
Zhi-Guo
ZG
Li
Xin-Jian
XJ
Fang
Bing
B
Yuan
Wu
W
Luo
Yong
Y
Zhou
Jing
J
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014635:Valproic Acid / Q000494:pharmacology*
D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; D012333:RNA, Messenger; D014635:Valproic Acid