title: M1-activated macrophages migration, a marker of aortic atheroma progression: a preclinical MRI study in mice.
abstract: BACKGROUND
  M1-activated Macrophages (M1M) play a major role in atherosclerotic lesions of aortic arch, promoting proinflammatory response. In vivo trafficking of M1M in aortic plaques is therefore critical.
  
  
  METHODS
  M1M from bone marrow cell culture were magnetically labeled, using iron nanoparticles, intravenously injected and followed up with 3 day magnetic resonance imaging (MRI) in mice developing macrophage-laden atheroma (ApoE2 knock-in mice). M1M recruitment in aortic arch lesions was assessed both by MRI and histology.
  
  
  RESULTS
  In all ApoE2 knock-in mice injected with labeled cells, high resolution MRI showed localized signal loss regions in the thickened aortic wall, with a maximal effect at day 2 (-34% +/- 7.3% P < 0.001 compared with baseline). This was confirmed with Prussian blue (iron) staining and corresponded to M1M (Major Histo-compatibility Complex II positive). Clear different intraplaque and adventitial dynamic distribution profiles of labeled cells were observed during the 3 days.
  
  
  CONCLUSIONS
  M1M dynamic MRI is a promising marker to noninvasively assess the macrophage trafficking underlying aortic arch plaque progression.
authors.affiliation: CREATIS-LRMN, UMR CNRS 5220, U630 INSERM, University Lyon 1, Villeurbanne Cedex, France.
authors.forename: Amine
authors.initials: A
authors.lastname: Bessaad
authors.forename: Monica
authors.initials: M
authors.lastname: Sigovan
authors.forename: Hasan
authors.initials: H
authors.lastname: Alsaid
authors.forename: Geneviève
authors.initials: G
authors.lastname: De Souza
authors.forename: Nicolas
authors.initials: N
authors.lastname: Provost
authors.forename: Zouher
authors.initials: Z
authors.lastname: Majd
authors.forename: Christine
authors.initials: C
authors.lastname: Menager
authors.forename: Jèrôme
authors.initials: J
authors.lastname: Honnorat
authors.forename: Florence
authors.initials: F
authors.lastname: Lagarde
authors.forename: Norbert
authors.initials: N
authors.lastname: Nighoghossian
authors.forename: Serge
authors.initials: S
authors.lastname: Nataf
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Canet-Soulas
mesh_terms: D000818:Animals; D001018:Aortic Diseases / Q000175:diagnosis*; D053329:Apolipoprotein E2 / Q000235:genetics; D001161:Arteriosclerosis / Q000175:diagnosis*; D015415:Biomarkers; D055879:Gene Knock-In Techniques; D008262:Macrophage Activation / Q000502:physiology*; D008264:Macrophages / Q000502:physiology; D008279:Magnetic Resonance Imaging; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D053329:Apolipoprotein E2; D015415:Biomarkers