title: CO I barcoding reveals new clades and radiation patterns of Indo-Pacific sponges of the family Irciniidae (Demospongiae: Dictyoceratida).
abstract: BACKGROUND
  DNA barcoding is a promising tool to facilitate a rapid and unambiguous identification of sponge species. Demosponges of the order Dictyoceratida are particularly challenging to identify, but are of ecological as well as biochemical importance.
  
  
  RESULTS
  Here we apply DNA barcoding with the standard CO1-barcoding marker on selected Indo-Pacific specimens of two genera, Ircinia and Psammocinia of the family Irciniidae. We show that the CO1 marker identifies several species new to science, reveals separate radiation patterns of deep-sea Ircinia sponges and indicates dispersal patterns of Psammocinia species. However, some species cannot be unambiguously barcoded by solely this marker due to low evolutionary rates.
  
  
  CONCLUSIONS
  We support previous suggestions for a combination of the standard CO1 fragment with an additional fragment for sponge DNA barcoding.
authors.affiliation: Museum für Naturkunde, Department of Malacozoology, Leibniz Institute for Research on Evolution and Biodiversity at the Humboldt University Berlin, Berlin, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Pöppe
authors.forename: Patricia
authors.initials: P
authors.lastname: Sutcliffe
authors.forename: John N A
authors.initials: JN
authors.lastname: Hooper
authors.forename: Gert
authors.initials: G
authors.lastname: Wörheide
authors.forename: Dirk
authors.initials: D
authors.lastname: Erpenbeck
mesh_terms: D000818:Animals; D005075:Biological Evolution; D004247:DNA / Q000032:analysis*; D003576:Electron Transport Complex IV / Q000235:genetics*; D001330:Electronic Data Processing / Q000379:methods*; D005819:Genetic Markers; D009792:Oceans and Seas; D011161:Porifera / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D004247:DNA; D003576:Electron Transport Complex IV