Nanoscale sensor design via in situ labeling of gold nanoparticles onto protein scaffolds.
A one-step method for synthesis of bioconjugated gold nanoparticles is reported. A non-toxic and biocompatible phosphorus based reducing agent was used for reduction of gold (III) and formation of nanoparticles. Physicochemical properties of protein-A stabilized gold nanoparticls were investigated. Result of immunoassay experiments confirmed the potential of the synthesized anti-protein-A conjugated gold nanoparticles for use as a simple and inexpensive test for quantitative screening of protein-A samples.
Afrasiabi
Zahra
Z
Department of Life and Physical Sciences, Lincoln University, Jefferson City, MO 65101, USA.
Shukla
Ravi
R
Chanda
Nripen
N
Bhaskaran
Sharanya
S
Upendran
Anandhi
A
Zambre
Ajit
A
Katti
Kattesh V
KV
Kannan
Raghuraman
R
D006046:Gold; D053768:Metal Nanoparticles; D036103:Nanotechnology; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D006046:Gold