title: Molecular characterization of echovirus 30-associated outbreak of aseptic meningitis in Korea in 2008.
abstract: Evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. In Korea, an outbreak of aseptic meningitis caused by echovirus type 30 (E30) occurred from May to October in 2008. In order to determine the etiologic agent, CSF and/or stool specimens from 140 children hospitalized for aseptic meningitis at Soonchunhyang University Cheonan Hospital between June and October of 2008 were tested for virus isolation and identification. E30 accounted for 61.7% (37 cases) and echovirus 6 accounted for 21.7% (13 cases) of all the human enteroviruses (HEVs) isolates (60 cases in total). For the molecular characterization of the isolates, the VP1 gene sequence of 18 Korean E30 isolates was compared pairwise using the MegAlign with 34 reference strains from the GenBank database. The pairwise comparison of the nucleotide sequences of the VP1 genes demonstrated that the sequences of the Korean strains differed from those of lineage groups A, B, C, D, E, F and G. Reconstruction of the phylogenetic tree based on the complete VP1 nucleotide sequences resulted in a monophyletic tree, with eight clustered lineage groups. All Korean isolates were segregated from other lineage groups, thus suggesting that the Korean strains were a distinct lineage of E30, and a probable cause of this outbreak. This manuscript is the first report, to the best of our knowledge, of the molecular characteristics of E30 strains associated with an aseptic meningitis outbreak in Korea, and their respective phylogenetic relationships.
authors.affiliation: Department of Clinical Pathology, Soonchunhyang University Cheonan Hospital, Cheonan 330-721, Korea.
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Choi
authors.forename: Kwi Sung
authors.initials: KS
authors.lastname: Park
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Baek
authors.forename: Eun Hye
authors.initials: EH
authors.lastname: Jung
authors.forename: Hae Seon
authors.initials: HS
authors.lastname: Nam
authors.forename: Yong Bae
authors.initials: YB
authors.lastname: Kim
authors.forename: Joon Soo
authors.initials: JS
authors.lastname: Park
mesh_terms: D000293:Adolescent; D000595:Amino Acid Sequence; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D004457:Echovirus Infections / Q000453:epidemiology* / Q000821:virology*; D029822:Enterovirus B, Human / Q000235:genetics / Q000254:growth & development*; D005260:Female; D006801:Humans; D007723:Korea / Q000453:epidemiology; D008297:Male; D008582:Meningitis, Aseptic / Q000453:epidemiology* / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D012367:RNA, Viral