title: Surface modification of magnetic nanoparticles using asparagines-serine polypeptide designed to control interactions with cell surfaces.
abstract: Surface modification is an important part of the fabrication of nanoparticles that have specific properties and functions. Here we describe the development of a functional polypeptide and a simple available technology for surface modification of nanoparticles. A NS polypeptide, which is 100 amino acids composed of repeated units of four asparagine and one serine residue (NS), as a molecule for nanoparticle surface modification was designed. Modification of the surface of a magnetic nanoparticle with the NS polypeptide results in reduction of particle-particle and particle-cell interactions. When NS polypeptide is used in single fusion protein as a linker to display protein G on nanoparticles, the nanoparticle acquires the capacity to specifically bind target cells and to avoid nonspecific adsorption of non-target cells. This technology, incorporating a functional polypeptide, may represent a completely new strategy for surface modification of nanoparticles for use in a variety of cell-associated applications.
authors.affiliation: Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yoshino
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsunaga
mesh_terms: D000818:Animals; D001216:Asparagine / Q000737:chemistry*; D001773:Blood Cells / Q000166:cytology / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002469:Cell Separation; D015870:Gene Expression; D006801:Humans; D008280:Magnetics; D055944:Magnetosomes / Q000378:metabolism* / Q000648:ultrastructure; D042201:Magnetospirillum / Q000235:genetics / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012694:Serine / Q000737:chemistry*; D013499:Surface Properties
chemical_list: C029152:G-substrate; D009419:Nerve Tissue Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; D012694:Serine; D001216:Asparagine