title: Bioreduction of some common carbonylic compounds mediated by yeasts.
abstract: Bioreduction of several prochiral carbonylic compounds such as acetophenone (1), ethyl acetoacetate (2) and ethyl phenylpropionate (3) to the corresponding optically active sec-alcohols 1a-3a was performed using wild-type strains of Pichia pastoris UBB 1500, Rhodotorula sp., and Saccharomyces cerevisiae. The reductions showed moderate to excellent conversion and high enantiomeric excess, in an extremely mild and environmentally benign manner in aqueous medium, using glucose as cofactor regeneration system. The obtained alcohols follow Prelog's rule, but in the reduction of 1 with P. pastoris UBB 1500 the anti-Prelog enantiopreference was observed.
authors.affiliation: Departamento de Organica, Facultad de Ciencias Químicas, Universidad de Concepción, Edmundo Larenas 129, Concepción, Chile.
authors.forename: Javier
authors.initials: J
authors.lastname: Silva
authors.forename: Julio
authors.initials: J
authors.lastname: Alarcón
authors.forename: Sergio A
authors.initials: SA
authors.lastname: Aguila
authors.forename: Joel B
authors.initials: JB
authors.lastname: Alderete
mesh_terms: D000090:Acetoacetates / Q000737:chemistry / Q000378:metabolism*; D000098:Acetophenones / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D010666:Phenylpropionates / Q000737:chemistry / Q000378:metabolism*; D010843:Pichia / Q000254:growth & development / Q000378:metabolism*; D012248:Rhodotorula / Q000254:growth & development / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000090:Acetoacetates; D000098:Acetophenones; D010666:Phenylpropionates; C469627:ethyl phenylpropanoate; C024840:ethyl acetoacetate; C038699:acetophenone