title: A novel profluorescent nitroxide as a sensitive probe for the cellular redox environment.
abstract: Changes to the redox status of biological systems have been implicated in the pathogenesis of a wide variety of disorders. Sensitive quantification of these changes has been developed using a novel fluorescent probe containing a redox-sensitive nitroxide moiety. As well as being able to selectively detect the superoxide radical in vitro, this method can measure overall changes to the cellular redox environment using flow cytometry on the basis of nitroxide reduction. The reversible nature of the probe's detection mechanism offers the unique advantage of being able to monitor redox changes in both oxidizing and reducing directions in real time.
authors.affiliation: ARC Centre of Excellence for Free Radical Chemistry, School of Physical and Chemical Sciences, Queensland University of Technology, Brisbane, QLD 4001, Australia.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Morrow
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Keddie
authors.forename: Nuri
authors.initials: N
authors.lastname: Gueven
authors.forename: Martin F
authors.initials: MF
authors.lastname: Lavin
authors.forename: Steven E
authors.initials: SE
authors.lastname: Bottle
mesh_terms: D015415:Biomarkers; D002461:Cell Line, Transformed; D002469:Cell Separation; D005347:Fibroblasts / Q000378:metabolism*; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D008856:Microscopy, Fluorescence; D009589:Nitrogen Oxides / Q000138:chemical synthesis*; D010084:Oxidation-Reduction
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D009589:Nitrogen Oxides; C039900:nitroxyl