Improvement of radiation-mediated immunosuppression of human NSCLC tumour xenografts in a nude rat model.
Human tumour xenografts in a nude rat model have consistently been used as an essential part of preclinical studies for anticancer drugs activity in human. Commonly, these animals receive whole body irradiation to assure immunosuppression. But whole body dose delivery might be inhomogeneous and the resulting incomplete bone marrow depletion may modify tumour behaviour. To improve irradiation-mediated immunosuppression of human non-small cell lung cancer (NSCLC) xenografts in a nude rat model irradiation (2 + 2 Gy) from opposite sides of animals has been performed using a conventional X-ray tube. The described modification of whole body irradiation improves growth properties of human NSCLC xenografts in a nude rat model. The design of the whole body irradiation mediated immunosuppression described here for NSCLC xenografts may be useful for research applications involving other types of human tumours.
Tokalov
Sergey V
SV
OncoRay-Center for Radiation Research in Oncology, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstrasse 74, P.O. Box 86, 01307 Dresden, Germany. sergey.tokalov@oncoray.de
Enghardt
Wolfgang
W
Abolmaali
Nasreddin
N
D000704:Analysis of Variance; D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D018450:Disease Progression; D006801:Humans; D007165:Immunosuppression Therapy / Q000379:methods*; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D008297:Male; D009010:Monte Carlo Method; D019047:Phantoms, Imaging; D051381:Rats; D011923:Rats, Nude; D014916:Whole-Body Irradiation / Q000379:methods*; D014965:X-Rays; D023041:Xenograft Model Antitumor Assays / Q000379:methods*