Broome virus, a new fusogenic Orthoreovirus species isolated from an Australian fruit bat.
This report describes the discovery and characterization of a new fusogenic orthoreovirus, Broome virus (BroV), isolated from a little red flying-fox (Pteropus scapulatus). The BroV genome consists of 10 dsRNA segments, each having a 3' terminal pentanucleotide sequence conserved amongst all members of the genus Orthoreovirus, and a unique 5' terminal pentanucleotide sequence. The smallest genome segment is bicistronic and encodes two small nonstructural proteins, one of which is a novel fusion associated small transmembrane (FAST) protein responsible for syncytium formation, but no cell attachment protein. The low amino acid sequence identity between BroV proteins and those of other orthoreoviruses (13-50%), combined with phylogenetic analyses of structural and nonstructural proteins provide evidence to support the classification of BroV in a new sixth species group within the genus Orthoreovirus.
Thalmann
Claudia M
CM
CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia.
Cummins
David Michael
DM
Yu
Meng
M
Lunt
Ross
R
Pritchard
Lindsay Ian
LI
Hansson
Eric
E
Crameri
Sandra
S
Hyatt
Alex
A
Wang
Lin-Fa
LF
D000595:Amino Acid Sequence; D000818:Animals; D001315:Australia; D002460:Cell Line; D002685:Chiroptera / Q000821:virology*; D016000:Cluster Analysis; D006224:Cricetinae; D016679:Genome, Viral; D008969:Molecular Sequence Data; D018165:Orthoreovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012088:Reoviridae Infections / Q000662:veterinary*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins