title: Initial biocompatibility and enhanced osteoblast response of Si doping in a porous BCP bone graft substitute.
abstract: Granular shape biphasic calcium phosphate (BCP) bone grafts with and without doping of silicon cations were evaluated in regards to biocompatibility and MG-63 cellular response. To do this we studied Cellular cytotoxicity, cellular adhesion and spreading behavior and cellular differentiation with alizarin red S staining. Gene expression in MG-63 cells on the implanted bone substitutes was also examined at different time points using RT-PCR. In comparison, the Si-doped BCP granule showed more cellular viability than the BCP granule without doping in MTT assay. Moreover, cell proliferation was much higher when Si doping was employed. The cells grown on the silicon-doped BCP substitutes had more active filopodial growth with cytoplasmic webbing that proceeded to the flattening stage, which was indicative of well cellular adhesion. When these cells were exposed to Si-doped BCP granules for 14 days, well differentiated MG-63 cells were observed. Osteonectin and osteopontin genes were highly expressed in the late stage of differentiation (14 days), whereas collagen type I mRNA were found to be highly expressed during the early stage (day 3). These combined results of this study demonstrate that silicon-doped BCP enhanced osteoblast attachment/spreading, proliferation, differentiation and gene expression.
authors.affiliation: Department of Immunology, School of Medicine, Soonchunhyang University, Cheonan, Chungnum 330-090, South Korea.
authors.forename: In-Seon
authors.initials: IS
authors.lastname: Byun
authors.forename: Swapan Kumar
authors.initials: SK
authors.lastname: Sarkar
authors.forename: M
authors.initials: M
authors.lastname: Anirban Jyoti
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Min
authors.forename: Hyung-Seok
authors.initials: HS
authors.lastname: Seo
authors.forename: Byong-Taek
authors.initials: BT
authors.lastname: Lee
authors.forename: Ho-Yeon
authors.initials: HY
authors.lastname: Song
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000378:metabolism; D002130:Calcium Phosphates / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D016062:Porosity; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D024042:Collagen Type I; D015676:Osteonectin; D012333:RNA, Messenger; D053495:Osteopontin; C020243:calcium phosphate