title: Photodynamic effects of Radachlorin on cervical cancer cells.
abstract: OBJECTIVE
  Photodynamic therapy (PDT) is a novel treatment modality, which produces local tissue necrosis with laser light following the prior administration of a photosensitizing agent. Radachlorin has recently been shown to be a promising PDT sensitizer. In order to elucidate the antitumor effects of PDT using Radachlorin on cervical cancer, growth inhibition studies on a HPV-associated tumor cell line, TC-1 cells in vitro and animals with an established TC-1 tumor in vivo were determined.
  
  
  METHODS
  TC-1 tumor cells were exposed to various concentrations of Radachlorin and PDT, with irradiation of 12.5 or 25 J/cm(2) at an irradiance of 20 mW/cm(2) using a Won-PDT D662 laser at 662 nm in vitro. C57BL/6 mice with TC-1 tumor were injected with Radachlorin via different routes and treated with PDT in vivo. A growth suppression study was then used to evaluate the effects at various time points after PDT.
  
  
  RESULTS
  The results showed that irradiation of TC-1 tumor cells in the presence of Radachlorin induced significant cell growth inhibition. Animals with established TC-1 tumors exhibited significantly smaller tumor sizes over time when treated with Radachlorin and irradiation.
  
  
  CONCLUSIONS
  PDT after the application of Radachlorin appears to be effective against TC-1 tumors both in vitro and in vivo.
authors.affiliation: Catholic Research Institutes of Medical Science, The Catholic University of Korea College of Medicine, Seoul, Korea.
authors.forename: Su-Mi
authors.initials: SM
authors.lastname: Bae
authors.forename: Yong-Wook
authors.initials: YW
authors.lastname: Kim
authors.forename: Joon-Mo
authors.initials: JM
authors.lastname: Lee
authors.forename: Sung-Eun
authors.initials: SE
authors.lastname: Namkoong
authors.forename: Sei-Jun
authors.initials: SJ
authors.lastname: Han
authors.forename: Jong-Ki
authors.initials: JK
authors.lastname: Kim
authors.forename: Chang-Hee
authors.initials: CH
authors.lastname: Lee
authors.forename: Heung-Jae
authors.initials: HJ
authors.lastname: Chun
authors.forename: Hyun-Sun
authors.initials: HS
authors.lastname: Jin
authors.forename: Woong-Shick
authors.initials: WS
authors.lastname: Ahn