AtMYB12 gene: a novel visible marker for wheat transformation.
Efficiency of plant transformation is less than optimal for many important species, especially for monocots which are traditionally recalcitrant to transformation, such as wheat. And due to limited number of selectable marker genes, identification or selection of those cells that have integrated DNA into appropriate plant genome and to regenerate fully developed plants from the transformed cells, becomes even more difficult. Some of the widely used marker genes belong to the categories of herbicide or antibiotic resistance genes and flourescent protein genes. As they become an integral part of plant genome along with promoters prokaryotic or eukaryotic origin, there are certain health and environmental concerns about the use of these reporter genes. These marker genes are also inefficient with respect to time and space. In this study we have found a novel visible selection agent AtMYB12, to screen transgenic wheat, with in days after transformation. Transformed coleoptiles as well as cells regenerating from transformed cultured scutella, phenotypically exhibit purple pigmentation, making selection possible in limited and reasonable cost, time and space.
Gao
Xuan
X
China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, The Genetic Engineering International Cooperation Base of Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Luoyu Road 1037, 430074, Wuhan, China.
Zhang
Li
L
Zhou
Shiyi
S
Wang
Changdong
C
Deng
Xiaoming
X
Zhang
Hong
H
Yang
Guangxiao
G
Javeed
Hussain
H
He
Guangyuan
G
D000872:Anthocyanins / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics*; D019470:Biolistics; D015153:Blotting, Western; D018744:DNA, Plant / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism; D017343:Genes, Plant / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D005819:Genetic Markers; D005966:Glucuronidase / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D010858:Pigmentation / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics; D016133:Polymerase Chain Reaction; D012038:Regeneration / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics*; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics* / Q000502:physiology
D000872:Anthocyanins; D029681:Arabidopsis Proteins; D018744:DNA, Plant; D005819:Genetic Markers; C504539:MYB12 protein, Arabidopsis; D010636:Phenols; D010940:Plant Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D005966:Glucuronidase