Asna1/TRC40-mediated membrane insertion of tail-anchored proteins.
Tail-anchored (TA) proteins insert post-translationally into the membrane of the endoplasmic reticulum (ER) and span the membrane by their C-terminal transmembrane domain. We have reconstituted membrane insertion of TA proteins from recombinant Asna1/TA protein complexes and ER-derived membranes. Our data show that Asna1 can mediate membrane insertion of RAMP4 and Sec61beta without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive receptor in the ER membrane. By contrast, membrane insertion of cytochrome b5 can proceed independently of Asna1 and nucleotides.
Favaloro
Vincenzo
V
Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ - ZMBH Allianz, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
Vilardi
Fabio
F
Schlecht
Rainer
R
Mayer
Matthias P
MP
Dobberstein
Bernhard
B
D000818:Animals; D053501:Arsenite Transporting ATPases / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D015786:Cytochromes b5 / Q000378:metabolism; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D008970:Molecular Weight; D009711:Nucleotides / Q000378:metabolism; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D000069816:SEC Translocation Channels; D012995:Solubility
C489667:GET3 protein, human; D008565:Membrane Proteins; D009711:Nucleotides; D011993:Recombinant Fusion Proteins; D000069816:SEC Translocation Channels; D015786:Cytochromes b5; D053501:Arsenite Transporting ATPases