Development and application of a method for analysis of phthalates in ham sausages by solid-phase extraction and gas chromatography-mass spectrometry.
A gas chromatography-mass spectrometry assay was developed and successfully applied for the determination of phthalates in ham sausage migrated from packaging film. The phthalates studied were dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), benzylbutyl phthalate (BBP), bis(2-ethylhexyl) phthalate (DEHP) and di-n-octyl phthalate (DNOP), with dibutyl adipate (DBA) as internal standard. The sample pre-treatments included extraction with n-hexane, solvent evaporation and reconstitution with acetonitrile before and after solid-phase extraction (SPE). The extraction and cleaning up procedure was carried out with cartridges containing dimethyl butylamine groups, which showed extraction efficiencies over 87.3%. The calibration curves obtained were linear with correlation coefficients greater than 0.99. The method proved to be accurate and precise for the six phthalates used. It was successfully applied to a study on the migration of phthalates from packaging PVC film into ham sausage.
Guo
Zhiyong
Z
Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo, Zhejiang 315211, PR China. guozhiyong@nbu.edu.cn
Wang
Sui
S
Wei
Danyi
D
Wang
Meili
M
Zhang
Huina
H
Gai
Panpan
P
Duan
Jing
J
D000818:Animals; D052244:Endocrine Disruptors / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D018857:Food Packaging / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008461:Meat Products / Q000032:analysis*; D010795:Phthalic Acids / Q000032:analysis* / Q000633:toxicity; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods; D013552:Swine
D052244:Endocrine Disruptors; D010795:Phthalic Acids