Expression and ultrastructural localization of Mint2 in the spinal cord of rats.
Mint protein family, as adaptor molecules, contains three members, Mint1, Mint2 and Mint3. Although Mint3 is ubiquitously expressed, Mint1 and Mint2 have been reported to express specifically in neuron. Here we demonstrated Mint1 and Mint2 expression pattern in rat spinal cord. The protein level of Mint2 was found to be higher than that of Mint1 in rat spinal by western blot. In an attempt to know Mint2 distribution in the spinal cord of rat, in situ hybridization was carried out, Mint2 mRNA was showed to be ubiquitously distributed in cervical, thoracic and lumbar sections of rat spinal cord, and high intensive signal was detected in motor neurons. These were further confirmed by fluorescent immunohistochemistry, Mint2 was also found to exist throughout gray matter especially motor neurons where Mint2 was mainly located in perikaryon, however, Mint1 was showed to be relatively lower. By electron microscope, Mint2 was found to be mainly located in vesicles in perikaryon in motor neuron of lumbar section, and at the same time Mint2 was located in axons in myelin and presynaptic terminals. These data suggest that Mint2 may play more important role in spinal cord than the other two family members.
Yao
Ling-Ling
LL
Department of Physiology and Pathophysiology, Fudan University Shanghai Medical College, and Neurology Department of ShangHai Tenth People Hospital, Shanghai, 200032, China.
Liu
Xue-Yuan
XY
Jin
Ji-Yang
JY
Tao
Bei-Bei
BB
Chen
Yu-Juan
YJ
Yu
Yong-Chun
YC
Bian
Wei-Hong
WH
Yu
Jing
J
Huang
Jing
J
Wang
Yong-Gang
YG
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D015820:Cadherins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure*
D048868:Adaptor Proteins, Signal Transducing; C513071:Apba1 protein, rat; C109650:Apba2 protein, rat; D015820:Cadherins; D002352:Carrier Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger