Kinetics and role of antibodies against intimin beta in colostrum and in serum from goat kids and longitudinal study of attaching and effacing Escherichia coli in goat kids.
The presence of antibodies to the intimin beta-binding region (Int280-beta) of attaching and effacing Escherichia coli (AEEC) in serum from 20 goat kids from 2 herds, as well as in goat colostrum, was investigated by enzyme-linked immunosorbent assay. In addition, the onset and subsequent pattern of shedding of AEEC from the same goat kids over a 6-mo period was investigated. All the colostrum and serum samples tested contained antibodies against Int280-beta. The association between the antibody titer and the isolation of AEEC suggests that antibodies to intimin beta do not prevent colonization of the intestine by AEEC in goat kids. The AEEC were generally shed only transiently. Most AEEC isolated from the kids belonged to serogroup O26. Three isolates belonged to serogroup O157. These data show that goat kids may be a reservoir of AEEC that are potentially pathogenic for humans.
Orden
José A
JA
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain. jaorden@vet.ucm.es
De la Fuente
Ricardo
R
Yuste
María
M
Martínez-Pulgarín
Susana
S
Ruiz-Santa-Quiteria
José A
JA
Horcajo
Pilar
P
Contreras
Antonio
A
Sánchez
Antonio
A
Corrales
Juan C
JC
Domínguez-Bernal
Gustavo
G
D018829:Adhesins, Bacterial / Q000276:immunology*; D000375:Aging; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D003126:Colostrum / Q000276:immunology*; D004197:Disease Reservoirs / Q000382:microbiology; D019453:Escherichia coli O157 / Q000276:immunology* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000276:immunology*; D006041:Goats / Q000276:immunology*; D007700:Kinetics; D008137:Longitudinal Studies
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli