title: Human cytomegalovirus (HCMV) genotype populations in immunocompetent individuals during primary HCMV infection.
abstract: BACKGROUND
  Immunocompetent individuals can harbor multiple human cytomegalovirus (HCMV) genotypes. However, little is known about the genotype populations acquired during primary HCMV infection.
  
  
  OBJECTIVE
  The aim of this study was to assess the HCMV genotype populations present in the blood of non-immunocompromised patients experiencing primary HCMV infection.
  
  
  METHODS
  HCMV glycoprotein B (gB), glycoprotein H (gH), and UL10 genotyping was performed on HCMV-positive serum samples of 36 immunocompetent patients during primary infection by sensitive gB- and gH-genotype-specific real-time-PCR assays and by UL10 sequencing.
  
  
  RESULTS
  In all cases only one gB-gH-UL10 genotype was detected. In contrast, mixed-genotype infections were found in 4 of 14 immunocompetent patients experiencing HCMV reactivation/reinfection (P=0.004).
  
  
  CONCLUSIONS
  Thus, the data support the presumption that multiple HCMV genotypes in immunocompetent individuals are often a result of serial reinfection rather than primary coinfection with different strains.
authors.affiliation: Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria.
authors.forename: Irene
authors.initials: I
authors.lastname: Görzer
authors.forename: Heidrun
authors.initials: H
authors.lastname: Kerschner
authors.forename: Monika
authors.initials: M
authors.lastname: Redlberger-Fritz
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Puchhammer-Stöckl
mesh_terms: D001769:Blood / Q000821:virology; D003587:Cytomegalovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C499358:glycoprotein H, Human cytomegalovirus