Efficiency of mitochondrial DNA restriction analysis and RAPD-PCR to characterize yeasts growing on dry-cured Iberian ham at the different geographic areas of ripening.
The efficiency of mitochondrial DNA (mtDNA) restriction analysis and random amplification of polymorphic DNA (RAPD)-PCR to characterize yeasts growing on dry-cured Iberian ham was evaluated. Besides, the distribution of the main species and biotypes of yeasts in the different ripening areas of this product was investigated. MtDNA restriction analysis allowed yeast characterization at species and strain level. RAPD-PCR with the primers (GACA)(4) and (GAC)(5) was inappropriate for characterization at species level. Most of the mtDNA restriction patterns detected in dry-cured Iberian ham were consistent with Debaryomyces hansenii. Several yeasts biotypes were associated to specific geographic areas of dry-cured Iberian ham ripening.
Andrade
María J
MJ
Higiene y Seguridad Alimentaria, Facultad de Veterinaria, Universidad de Extremadura, Avda. de la Universidad, s/n, 10071 Cáceres, Spain.
Rodríguez
Mar
M
Casado
Eva
E
Córdoba
Juan J
JJ
D000818:Animals; D017931:DNA Primers; D004271:DNA, Fungal; D004272:DNA, Mitochondrial / Q000235:genetics*; D005516:Food Microbiology; D005843:Geography; D008460:Meat / Q000382:microbiology*; D018482:Muscle, Skeletal / Q000382:microbiology; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D015183:Restriction Mapping / Q000379:methods*; D004718:Saccharomycetales / Q000145:classification / Q000235:genetics; D013045:Species Specificity; D013552:Swine; D015003:Yeasts / Q000145:classification / Q000235:genetics*
D017931:DNA Primers; D004271:DNA, Fungal; D004272:DNA, Mitochondrial