[Expression of caspase-8, receptor interacting protein and nuclear factor-kappaBp65 in oral lichen planus].
OBJECTIVE
To investigate the expressions of caspase-8, receptor interacting protein (RIP) and nuclear factor (NF)-kappaBp65 in oral lichen planus (OLP) and their relationship with cell apoptosis.


METHODS
Immunohistochemical technique with SP method was used to detect the expressions of caspase-8, RIP and NF-kappaBp65 in 30 OLP cases and 15 normal oral mucosa specimens. Terminal deoxynucleotidyl transferase-mediated nucleotide shift enzyme (TdT) mediated d-UTP end labeling (TUNEL) was used for detecting the cell apoptotic index (AI) in 15 OLP cases and 5 nomal oral mucosa specimens.


RESULTS
Compared with the control group, the AI of epithelial cells (6.76 +/- 2.32) increased and the AI of lymphocytes (1.75 +/- 0.74)decreased in OLP (P < 0.01). The positive rate of caspase-8, RIP and NF-kappaBp65 of epithelial cells were 97% (29/30), 87% (26/30) and 93% (28/30) respectively, significantly higher in OLP than in normal control (P < 0.05). The positive rate of caspase-8, RIP and NF-kappaBp65 of lymphocytes were 100% (30/30, 90% (27/30) and 80% (24/30) respectively, significantly higher in OLP than in normal control (P < 0.01). A positive correlation was also observed between NF-kappaBp65 expression of lymphocytes and AI of epithelial cells.


CONCLUSIONS
Accelerated apoptosis of the keratinocytes and inhibition of lymphocyte apoptosis may coexist and contribute to the formation and progression of OLP. The over expression of caspase-8, RIP and NF-kappaBp65 in OLP may play a role in the pathogenesis of OLP.
Shi
Yong-jian
YJ
Department of Stomatology, Medical College of Jinan University, Guangzhou 510632, China.
Shen
Li-jia
LJ
Yin
Cao
C
D000328:Adult; D000368:Aged; D017209:Apoptosis; D053181:Caspase 8 / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology; D017676:Lichen Planus, Oral / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D053422:Receptor-Interacting Protein Serine-Threonine Kinases / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000378:metabolism*
C498143:RELA protein, human; D051996:Transcription Factor RelA; C506249:RIPK1 protein, human; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; C505530:CASP8 protein, human; D053181:Caspase 8