title: Biochemical characterization of an acid phosphatase from Thermus thermophilus.
abstract: A recombinant putative acid phosphatase from Thermus thermophilus was expressed and purified from Escherichia coli. The recombinant phosphatase displayed activities in a broad range of temperature, from 40 to 90 degrees C, with optimal temperature at 70 degrees C. In addition, the recombinant enzyme had activities in a wide range of pH, from 3.6 to 9.1, with optimal pH at 6 in acetate buffer and with optimal pH at 6.5 in Hepes buffer. Furthermore, it showed significant thermal stability and still possessed 44% residual activity after 70 degrees C treatment for 15 min. Moreover, the recombinant phosphatase showed broad substrates specificities for monophosphate esters, p-nitrophenyl phosphate (pNPP) being the most preferred substrate, and it was able to resist inhibition by sodium tartrate. Additionally, the recombinant protein formed stable oligomer under partially denatured conditions and required calcium ions for enzymic activity.
authors.affiliation: Institute of Biotechnology, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Sy-Jye
authors.initials: SJ
authors.lastname: Tham
authors.forename: Ching-Dong
authors.initials: CD
authors.lastname: Chang
authors.forename: Hao-Jen
authors.initials: HJ
authors.lastname: Huang
authors.forename: Yueh-Feng
authors.initials: YF
authors.lastname: Lee
authors.forename: Tze-Sing
authors.initials: TS
authors.lastname: Huang
authors.forename: Ching-Chun
authors.initials: CC
authors.lastname: Chang
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D009596:Nitrophenols; D009943:Organophosphorus Compounds / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D013644:Tartrates; D013696:Temperature; D016963:Thermus thermophilus / Q000201:enzymology* / Q000378:metabolism
chemical_list: D009596:Nitrophenols; D009943:Organophosphorus Compounds; D011994:Recombinant Proteins; D013644:Tartrates; C008644:nitrophenylphosphate; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; C029768:tartaric acid