Comparative effects of two different artificial body fluids on Candida albicans adhesion to soft lining materials.
This study investigated the C. albicans adhesion to cold- and heat-polymerized soft lining materials that were initially incubated in two different artificial body fluids, namely saliva and nasal secretion, and examined the surface roughness the materials (cold and heat polymerized soft liner) tested in vitro. Cold (Visco Gel) and heat-polymerized (Molloplast B) soft liner specimens (N=32, n=8 per group) (10x10x1.5 mm) were randomly produced to express the relationship between surface roughness and contamination, and influence of body fluids, and incubated in 1.5 ml contaminated solutions for 2 h. After fixation, all of materials were evaluated under optical microscope (x400) and SEM. Surface roughness measurements were examined with profilometre for each material. Data were analyzed using two-way ANOVA, Tukey's HSD and Dunnett T3 tests (alpha=0.05). Material type (p<0.05) and contamination media (p<0.05) showed a significant influence on the C. albicans adherence. The surface roughness of cold polymerized soft liner (Visco Gel) was significantly higher than heat-polymerized soft liner (Molloplast B) (p<0.05).
Vural
Caner
C
Department of Biology, Microbiology Section, Faculty of Science, Ege University, Izmir, Turkey.
Ozdemir
Guven
G
Kurtulmus
Huseyin
H
Kumbuloglu
Ovul
O
Ozcan
Mutlu
M
D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D002712:Chlorides / Q000737:chemistry; D004396:Coloring Agents; D003764:Dental Materials / Q000737:chemistry*; D003822:Denture Liners / Q000382:microbiology*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008751:Methylene Blue; D008768:Methylmethacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D009297:Nasal Mucosa / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D011188:Potassium / Q000737:chemistry; D012464:Saliva, Artificial / Q000737:chemistry; D012826:Silicone Elastomers / Q000737:chemistry*; D012964:Sodium / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
D002712:Chlorides; D004396:Coloring Agents; D003764:Dental Materials; D004129:Dimethylpolysiloxanes; C020964:MDX 4-4210; D008768:Methylmethacrylates; D011108:Polymers; D012464:Saliva, Artificial; D012826:Silicone Elastomers; C009931:poly(ethylmethacrylate); D012964:Sodium; D011188:Potassium; D008751:Methylene Blue