Detection and quantification of some plant growth regulators in a seaweed-based foliar spray employing a mass spectrometric technique sans chromatographic separation.
The sap expelled from the fresh harvest of Kappaphycus alvarezii , a red seaweed growing in tropical waters, has been reported to be a potent foliar spray. Tandem mass spectrometry of various organic extracts of the sap confirmed the presence of the plant growth regulators (PGRs) indole 3-acetic acid, gibberellin GA(3), kinetin, and zeatin. These PGRs were quantified in fresh state and after 1 year of storage by ESI-MS without recourse to chromatographic separation. Quantification was validated against HPLC data. The results may be useful in correlating with the efficacy of the sap. The methodology was extended to two other seaweeds. The method developed is convenient and precise and may find application in other agricultural formulations containing these growth hormones.
Prasad
Kamalesh
K
Central Salt and Marine Chemicals Research Institute (Council of Scientific and Industrial Research), G. B. Marg, Bhavnagar, Gujarat, India. kamlesh@csmcri.org
Das
Arun Kumar
AK
Oza
Mihir Deepak
MD
Brahmbhatt
Harshad
H
Siddhanta
Arup Kumar
AK
Meena
Ramavatar
R
Eswaran
Karuppanan
K
Rajyaguru
Mahesh Rameshchandra
MR
Ghosh
Pushpito Kumar
PK
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010937:Plant Growth Regulators / Q000032:analysis*; D012624:Seaweed / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D010937:Plant Growth Regulators