title: Polymorphisms in the apoptosis-associated genes FAS and FASL and risk of oral cancer and malignant potential of oral premalignant lesions in a Taiwanese population.
abstract: BACKGROUND
  Our aim was to measure the relationship of FAS (-1377G>A and -670A>G), FASL (-844C>T) gene variants and risk of oral cancer.
  
  
  METHODS
  Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used to determine the FAS and FASL polymorphisms in 294 oral squamous cell carcinoma (OSCC), 53 oral submucous fibrosis (OSF), and 84 oral leukoplakia (OL) patients, as well as in 333 healthy controls. A standardized questionnaire was applied to collect demographic data, and potential confounding factors. JMP statistical software was used to analyze the association.
  
  
  RESULTS
  FAS and FASL polymorphisms were not correlated with OSCC development or the malignant potential of OL by simple and multivariate logistic regression. However, a two- to fourfold difference in the risks of betel quid chewing, alcohol consumption, and smoking on OSCC development were observed between participants with different FAS polymorphisms. FAS polymorphisms were significantly correlated with the malignant potential of OSF. Multivariate logistic regression analysis indicated that FAS A(-1377)-G(-670) vs. G(-1377)-A(-670) haplotype (OR = 2.26, 95% CI = 1.16-4.41) was correlated with the malignant potential of OSF.
  
  
  CONCLUSIONS
  We suggest that FAS and FASL polymorphisms are not significantly correlated with OSCC development or malignant potential of OL. The impact of substance usage on OSCC development could be differentiated by FAS polymorphisms. FAS A(-1377)-G(-670) haplotype may play a role in the malignant potential of OSF.
authors.affiliation: Division of Molecular Diagnosis, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
authors.forename: Li-Hsuan
authors.initials: LH
authors.lastname: Wang
authors.forename: Shuo-Chun
authors.initials: SC
authors.lastname: Ting
authors.forename: Chung-Ho
authors.initials: CH
authors.lastname: Chen
authors.forename: Chi-Cheng
authors.initials: CC
authors.lastname: Tsai
authors.forename: Oliver
authors.initials: O
authors.lastname: Lung
authors.forename: Ta-Chih
authors.initials: TC
authors.lastname: Liu
authors.forename: Chia-Wen
authors.initials: CW
authors.lastname: Lee
authors.forename: Yen-Yun
authors.initials: YY
authors.lastname: Wang
authors.forename: Chin-Ling
authors.initials: CL
authors.lastname: Tsai
authors.forename: Ying-Chu
authors.initials: YC
authors.lastname: Lin
mesh_terms: D000241:Adenosine; D000428:Alcohol Drinking; D017209:Apoptosis / Q000235:genetics*; D001114:Areca; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D003596:Cytosine; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D014644:Genetic Variation / Q000235:genetics; D006147:Guanine; D006239:Haplotypes; D006801:Humans; D007972:Leukoplakia, Oral / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D009914:Oral Submucous Fibrosis / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011230:Precancerous Conditions / Q000235:genetics*; D012307:Risk Factors; D012907:Smoking; D013624:Taiwan; D013941:Thymine; D019014:fas Receptor / Q000235:genetics*
chemical_list: C491901:FAS protein, human; D053222:Fas Ligand Protein; D019014:fas Receptor; D006147:Guanine; D003596:Cytosine; D000241:Adenosine; D013941:Thymine