[Evaluation of the GenoType MTBDRplus assay for rifampin and isoniazid susceptibility testing of Mycobacterium tuberculosis].
OBJECTIVE
to evaluate the ability of GenoType MTBDRplus Assay for detection of rifampin (RFP) and isoniazid (INH) resistance in Mycobacterium tuberculosis.


METHODS
seventy-five clinical isolates were tested for traditional drug susceptibility testing and GenoType MTBDRplus assay. The results were compared to assess the sensitivity and specificity of GenoType MTBDRplus assay.


RESULTS
the sensitivity of GenoType MTBDRplus assays for detection of RFP and INH resistance were 98.0% and 86.5%, respectively. In pan-susceptible isolates of Mycobacterium tuberculosis, the specificity was 100% for both RFP resistance and INH resistance.


CONCLUSIONS
with its high sensitivity and specificity, GenoType MTBDRplus assay is a promising rapid method to detect INH and RFP resistant Mycobacterium tuberculosis strains in Shanghai.
Gui
Xiao-Hong
XH
Department of Tuberculosis, Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336, China.
Xu
Peng
P
Zhao
Ming
M
Wang
Li-Li
LL
Wu
Jie
J
Li
Jing
J
Gao
Qian
Q
Mei
Jian
J
D000995:Antitubercular Agents / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D012293:Rifampin / Q000494:pharmacology; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000382:microbiology*
D000995:Antitubercular Agents; D011933:Reagent Kits, Diagnostic; D007538:Isoniazid; D012293:Rifampin