title: Microtubule-mediated transport of the tumor-suppressor protein Merlin and its mutants.
abstract: The neurofibromatosis type 2 (NF2) tumor-suppressor protein Merlin is a member of the ERM family of proteins that links the cytoskeleton to the plasma membrane. In humans, mutations in the NF2 gene cause neurofibromatosis type-2 (NF2), a cancer syndrome characterized by the development of tumors of the nervous system. Previous reports have suggested that the subcellular distribution of Merlin is critical to its function, and that several NF2 mutants that lack tumor-suppressor activity present improper localization. Here we used a Drosophila cell culture model to study the distribution and mechanism of intracellular transport of Merlin and its mutants. We found that Drosophila Merlin formed cytoplasmic particles that move bidirectionally along microtubules. A single NF2-causing amino acid substitution in the FERM domain dramatically inhibited Merlin particle movement. Surprisingly, the presence of this immotile Merlin mutant also inhibited trafficking of the WT protein. Analysis of the movement of WT protein using RNAi and pull-downs showed that Merlin particles are associated with and moved by microtubule motors (kinesin-1 and cytoplasmic dynein), and that binding of motors and movement is regulated by Merlin phosphorylation. Inhibition of Merlin transport by expression of the dominant-negative mutant or depletion of kinesin-1 results in increased nuclear accumulation of the transcriptional coactivator Yorkie. These results demonstrate the requirement of microtubule-dependent transport for Merlin function.
authors.affiliation: Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
authors.forename: Lorena B
authors.initials: LB
authors.lastname: Benseñor
authors.forename: Kari
authors.initials: K
authors.lastname: Barlan
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Rice
authors.forename: Richard G
authors.initials: RG
authors.lastname: Fehon
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Gelfand
mesh_terms: D000818:Animals; D001692:Biological Transport; D003208:Concanavalin A / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism*; D004398:Dyneins / Q000378:metabolism*; D004705:Endocytosis; D049452:Green Fluorescent Proteins / Q000378:metabolism; D016547:Kinesins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D008954:Models, Biological; D025581:Neurofibromin 2 / Q000378:metabolism* / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D015534:Trans-Activators / Q000378:metabolism; D000091102:YAP-Signaling Proteins
chemical_list: D029721:Drosophila Proteins; D025581:Neurofibromin 2; D009687:Nuclear Proteins; D015534:Trans-Activators; D000091102:YAP-Signaling Proteins; C503814:Yki protein, Drosophila; C099885:merlin, Drosophila; D003208:Concanavalin A; D049452:Green Fluorescent Proteins; D004398:Dyneins; D016547:Kinesins