title: Development of a rapid, cost-effective TaqMan Real-Time PCR Assay for identification and differentiation of Coccidioides immitis and Coccidioides posadasii.
abstract: Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.
authors.affiliation: Translational Genomics Research Institute, Pathogen Genomics Division (TGen North), 3051 West Shamrell Boulevard, Flagstaff, Arizona, USA.
authors.forename: Kelly W
authors.initials: KW
authors.lastname: Sheff
authors.forename: Emily R
authors.initials: ER
authors.lastname: York
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Driebe
authors.forename: Bridget M
authors.initials: BM
authors.lastname: Barker
authors.forename: Steven D
authors.initials: SD
authors.lastname: Rounsley
authors.forename: Victor G
authors.initials: VG
authors.lastname: Waddell
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Beckstrom-Sternberg
authors.forename: James S
authors.initials: JS
authors.lastname: Beckstrom-Sternberg
authors.forename: Paul S
authors.initials: PS
authors.lastname: Keim
authors.forename: David M
authors.initials: DM
authors.lastname: Engelthaler
mesh_terms: D003045:Coccidioides / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D003047:Coccidioidomycosis / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D013997:Time Factors