title: The polymorphisms of bovine VEGF gene and their associations with growth traits in Chinese cattle.
abstract: PCR-SSCP and DNA sequencing methods were employed to screen the genetic variation of VEGF gene in 671 individuals belonging to three Chinese indigenous cattle breeds including Nanyang, Jiaxian Red and Qinchuan. Three haplotypes (A, B and C), four observed genotypes (AA, AB, BB and AC) and three new SNPs (6765T>C ss130456744, 6860A>G ss130456745, 6893T>C ss130456746) were detected. The analysis suggested that one SNP (ss130456744) in the bovine VEGF gene had significant effects on birth weight, body weight and heart girth at 6 months old in the Nanyang breed (P < 0.05). The results showed that the SNP (ss130456744) in intron 2 of the VEGF gene is associated with early development and growth of Chinese cattle. These findings raise hope that this polymorphism can be a molecular breeding marker in breeding strategies through marker assisted selection (MAS) in Chinese domestic cattle.
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Pang
authors.forename: Juqiang
authors.initials: J
authors.lastname: Wang
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.forename: Chuzhao
authors.initials: C
authors.lastname: Lei
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Wangping
authors.initials: W
authors.lastname: Yue
authors.forename: Chuanwen
authors.initials: C
authors.lastname: Gu
authors.forename: Danxia
authors.initials: D
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D007438:Introns; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D013045:Species Specificity; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D017931:DNA Primers; D042461:Vascular Endothelial Growth Factor A