title: Evaluation of a new enzyme-linked immunosorbent assay to detect keratan sulfate in equine serum.
abstract: This study aimed to evaluate a system that identifies cartilage turn over and/or degradation through measurement of a new keratan sulfate (KS) epitope concentration in equine sera. Blood samples were collected from 30 horses, 1 (n = 15) and 2 year-olds (n = 15). Serum samples were analyzed for an epitope of keratan sulfate by 1/20/5D4 (KS5D4) and new epitopes of keratan sulfate using high sensitive keratan sulfate (HSKS), measured by two respective enzyme-linked immunosorbant assays (ELISAs). There was no correlation in serum concentration of KS evaluated using 5D4 and HSKS. Age had no significant effect on concentrations of KS measured with KS5D4 while 1 year-old horses showed significantly higher amounts than 2 year-olds with HSKS. Results suggest that HSKS could detect early signs of cartilage metabolic changes.
authors.affiliation: Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Vivien
authors.initials: V
authors.lastname: Lettry
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Sugaya
authors.forename: Kenji
authors.initials: K
authors.lastname: Hosoya
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takagi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okumura
mesh_terms: D000375:Aging; D000818:Animals; D002358:Cartilage, Articular / Q000033:anatomy & histology / Q000254:growth & development; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D000939:Epitopes / Q000097:blood; D006736:Horses; D007632:Keratan Sulfate / Q000097:blood*
chemical_list: D000939:Epitopes; D007632:Keratan Sulfate