Novel SNPs of butyrophilin (BTN1A1) and milk fat globule epidermal growth factor (EGF) 8 (MFG-E8) are associated with milk traits in dairy goat.
Butyrophilin (BTN1A1) and milk fat globule epidermal growth factor (EGF) 8 (MFG-E8) genes are both milk fat globule membrane proteins. BTN1A1 plays a key role in the secretion of milk lipid and production which has effects on performance traits, while the MFG-E8 is vital for the development of the mammary gland and phagocytic clearance of apoptotic cells. Therefore, BTN1A1 and MFG-E8 gene are candidate genes for quantitative traits in mammalian animals with respect to milk performance traits. The objective of this study is to investigate variations in goat BTN1A1 and MFG-E8 gene and analyze their associations with growth trait and milk performance. In this study, the goat BTN1A1 gene showed a novel single-nucleotide polymorphism (SNP): XM_001494179:g.8659C>T, resulting in a missense mutation: CTT (Leu)>TTT (Phe) at position 377 aa of the BTN1A1 (526 aa); the goat MFG-E8 gene showed four novel SNPs: NC_007319: g.843delA, 6417delC, 14892T>C and 14996A>C, only the 14892T>C result in a synonymous mutation. The associations between genotypes and production traits were analyzed. Significant statistical results implied that HinfI locus of BTN1A1 gene is associated with milk fat yield (P=0.004), total solid (P=0.002), solid-non fat (P=0.018) and first milk yield (P=0.030). The DA and EcoRV loci of MFG-E8 gene are associated with milk fat yield (DA locus: P=0.000; EcoRV locus: P=0.033) and total solid (DA locus: P=0.002; EcoRV locus: P=0.015) in the Xinong Saanen dairy goat.
Qu
Yujiao
Y
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China. quyujiao0112@yahoo.cn
Liu
Yanli
Y
Ma
Liang
L
Sweeney
Sandra
S
Lan
Xianyong
X
Chen
Zhongqi
Z
Li
Zhuanjian
Z
Lei
Chuzhao
C
Chen
Hong
H
D000818:Animals; D001947:Breeding; D000071797:Butyrophilins; D003612:Dairying; D004815:Epidermal Growth Factor / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D056426:Genetic Loci / Q000235:genetics; D006017:Glycolipids / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D006041:Goats / Q000235:genetics*; D066292:Lipid Droplets; D008562:Membrane Glycoproteins / Q000235:genetics*; D008892:Milk / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D019655:Quantitative Trait, Heritable
D000071797:Butyrophilins; D006017:Glycolipids; D006023:Glycoproteins; D008562:Membrane Glycoproteins; C110367:milk fat globule; D004815:Epidermal Growth Factor