title: Efficient online transcription factor binding site adjustment by integrating transitive graph projection with MoRAine 2.0.
abstract: We investigated the problem of imprecisely determined prokaryotic transcription factor (TF) binding sites (TFBSs). We found that the identification and reinvestigation of questionable binding motifs may result in improved models of these motifs. Subsequent modelbased predictions of gene regulatory interactions may be performed with increased accuracy when the TFBSs annotation underlying these models has been re-adjusted. We present MoRAine 2.0, a significantly improved version of MoRAine. It can automatically identify cases of unfavorable TFBS strand annotations and imprecisely determined TFBS positions. With release 2.0, we close the gap between reasonable running time and high accuracy. Furthermore, it requires only minimal input from the user: (1) the input TFBS sequences and (2) the length of the flanking sequences.
  
  
  CONCLUSIONS
  MoRAine 2.0 is an easy-to-use, integrated, and publicly available web tool for the re-annotation of questionable TFBSs. It can be used online or downloaded as a stand-alone version from http://moraine.cebitec.uni-bielefeld.de.
authors.affiliation: Genome Informatics, Bielefeld University, Bielefeld, Germany. Tobias.Wittkop@CeBiTec.Uni-Bielefeld.DE
authors.forename: Tobias
authors.initials: T
authors.lastname: Wittkop
authors.forename: Sven
authors.initials: S
authors.lastname: Rahmann
authors.forename: Jan
authors.initials: J
authors.lastname: Baumbach
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D019295:Computational Biology / Q000379:methods*; D020407:Internet; D008969:Molecular Sequence Data; D056510:Position-Specific Scoring Matrices; D012984:Software; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D014157:Transcription Factors