title: Sintering photonic beads for multiplex biosensing.
abstract: Monodispersed silica nanoparticles were assembled into opaline photonic crystal beads and cemented together by sintering in order to enhance the mechanical stability of the beads, which simulates the formation of nature opal. The sintering temperatures and structures of the sintered beads were investigated. The results showed that by sintering treatment at temperatures between 700 degrees C and 1000 degrees C for 2 hours the beads were more reliable for multiplex bioassay applications in mechanical robustness, fluorescent background, sensitivity and encoding ability. It is anticipated that the opal-like beads have wide applications in multiplex protein and nucleic acid analysis.
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Zhao
authors.forename: Yuanjin
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.forename: Ming
authors.initials: M
authors.lastname: Xu
authors.forename: Wenju
authors.initials: W
authors.lastname: Zhao
authors.forename: Zhongze
authors.initials: Z
authors.lastname: Gu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D006358:Hot Temperature; D055595:Mechanical Phenomena; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009841:Oligonucleotides / Q000737:chemistry; D055095:Optics and Photonics / Q000379:methods*; D016062:Porosity; D011506:Proteins / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D011506:Proteins; D012822:Silicon Dioxide