title: Rapid determination of melamine and cyanuric acid in milk powder using direct analysis in real time-time-of-flight mass spectrometry.
abstract: The use of fast semi-automated method employing direct analysis in real time (DART) ion source coupled to time-of-flight mass spectrometry (TOFMS) for analysis of melamine (MEL) and cyanuric acid (CYA) in milk powder and milk based products has been demonstrated in this study. Simple sample extraction procedure employing methanol-5% aqueous formic acid mixture, which enabled disruption of melamine-cyanurate complex, was followed by direct, high-throughput (30s per run) examination of sample extracts spread on a glass rod by mass spectrometry under ambient conditions, without any prior chromatographic separation. After optimization of instrument parameter settings, limits of detection (LODs) 170 and 450microgkg(-1) were achieved for MEL and CYA, respectively. In the final phase of study, the possibility of minimizing spectral interference, thus improving method performance characteristics through the use of ultrahigh resolving power offered by Orbitrap based mass analyzer is demonstrated.
authors.affiliation: Institute of Chemical Technology Prague, Faculty of Food and Biochemical Technology, Department of Food Chemistry and Analysis, Technická 5, 16628 Prague 6, Czech Republic.
authors.forename: Lukas
authors.initials: L
authors.lastname: Vaclavik
authors.forename: Jan
authors.initials: J
authors.lastname: Rosmus
authors.forename: Bert
authors.initials: B
authors.lastname: Popping
authors.forename: Jana
authors.initials: J
authors.lastname: Hajslova
mesh_terms: D000818:Animals; D002417:Cattle; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D011208:Powders / Q000032:analysis; D014227:Triazines / Q000032:analysis*
chemical_list: D011208:Powders; D014227:Triazines; C004632:cyanuric acid; C011907:melamine