Prevention of Chinese sacbrood virus infection in Apis cerana using RNA interference.
Chinese sacbrood virus (CSBV) is the pathogen of Chinese sacbrood disease, which poses a serious threat to honeybee Apis cerana, and tends to cause bee colony and even the whole apiary collapse. Here we report on prevention of CSBV infection by feeding second instar larvae of A. cerana with specific sequences of CSBV double-stranded RNA (dsRNA). Protection of the bee larvae from CSBV by ingestion of CSBV-derived dsRNA was further demonstrated by quantitative real-time PCR (qRT-PCR) and northern blot analysis. The result provides a potential method to protect A. cerana from CSBV infection.
Liu
Xuejiao
X
South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China.
Zhang
Yi
Y
Yan
Xun
X
Han
Richou
R
D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000821:virology*; D015152:Blotting, Northern; D056631:Colony Collapse / Q000821:virology; D015870:Gene Expression; D007814:Larva / Q000821:virology; D010849:Picornaviridae / Q000235:genetics* / Q000472:pathogenicity*; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D013696:Temperature
D012330:RNA, Double-Stranded; D012367:RNA, Viral