Effects of adipose tissue-derived stem cell therapy after myocardial infarction: impact of the route of administration.
BACKGROUND
Cell-based therapies offer a promising approach to reducing the short-term mortality rate associated with heart failure after a myocardial infarction. The aim of the study was to analyze histological and functional effects of adipose tissue-derived stem cells (ADSCs) after myocardial infarction and compare 2 types of administration pathways.


RESULTS
ADSCs from 28 pigs were labeled by transfection. Animals that survived myocardial infarction (n = 19) received: intracoronary culture media (n = 4); intracoronary ADSCs (n = 5); transendocardial culture media (n = 4); or transendocardial ADSCs (n = 6). At 3 weeks' follow-up, intracoronary and transendocardial administration of ADSCs resulted in similar rates of engrafted cells (0.85 [0.19-1.97] versus 2 [1-2] labeled cells/cm(2), respectively; P = NS) and some of those cells expressed smooth muscle cell markers. The intracoronary administration of ADSCs was more effective in increasing the number of small vessels than transendocardial administration (223 +/- 40 versus 168 +/- 35 vessels/mm(2); P < .05). Ejection fraction was not modified by stem cell therapy.


CONCLUSIONS
This is the first study to compare intracoronary and transendocardial administration of autologous ADSCs in a porcine model of myocardial infarction. Both pathways of ADSCs delivery are feasible, producing a similar number of engrafted and differentiated cells, although intracoronary administration was more effective in increasing neovascularization.
Rigol
Montserrat
M
Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain. mrigol@clinic.ub.es
Solanes
Núria
N
Farré
Jordi
J
Roura
Santiago
S
Roqué
Mercè
M
Berruezo
Antonio
A
Bellera
Neus
N
Novensà
Laura
L
Tamborero
David
D
Prat-Vidal
Cristina
C
Huzman
M A Angeles
MA
Batlle
Montserrat
M
Hoefsloot
Margo
M
Sitges
Marta
M
Ramírez
José
J
Dantas
Ana Paula
AP
Merino
Anna
A
Sanz
Ginés
G
Brugada
Josep
J
Bayés-Genís
Antoni
A
Heras
Magda
M
D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D002478:Cells, Cultured; D004699:Endocardium / Q000473:pathology / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D009203:Myocardial Infarction / Q000473:pathology / Q000601:surgery*; D033581:Stem Cell Transplantation / Q000379:methods*; D013552:Swine; D013997:Time Factors