SYPRO Orange fluorescent staining of protein gels.
This method for staining protein gels uses a fluorescent dye, SYPRO Orange, which is very sensitive to protein (4-10 ng protein/band). SYPRO Orange cannot be visualized with the naked eye and thus a fluorescence scanner is required for detection of protein bands (the excitation maxima [Ex] are 300/472 nm and the emission maximum [Em] is 570 nm). SYPRO Red fluorescent protein stain uses the same binding principle as SYPRO Orange; hence, this protocol can also be used for SYPRO Red stain (Ex are 300/550 nm and Em is 630 nm). This staining method is mass-spectrometer-compatible.
Simpson
Richard J
RJ
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D009942:Organometallic Compounds / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D011506:Proteins; C515601:Sypro Ruby