Binding of semenogelin I to intact human spermatozoa studied by flow cytometry and surface plasmon resonance.
Approximately 1 in 10 couples is infertile. No definite cause can be found in about 25% of those cases. Studies have indicated that seminal vesicle secretion functions as an optimizer of fertilization. The Zn(2+) binding protein semenogelin I (SgI) represents a major fraction of the proteins present in seminal vesicle fluid, and it serves as a structural component of the coagulum that is formed after ejaculation. Cleavage of SgI by prostate-specific antigen results in liquefaction of the coagulum. Fragmented SgI has antibacterial effects and inhibits spermatozoa mobility. SgI has also been found complexed to eppin on spermatozoa, and this complex has been suggested to be of importance for fertility. Here, we used flow cytometry and surface plasmon resonance to study SgI regarding its association with spermatozoa and the interaction dependency on Zn(2+). The concentration of Zn(2+) in seminal plasma is approximately 100 times higher than in blood plasma, and the metal ion is known to change the structure of SgI. We found that SgI binds to spermatozoa in a concentration-dependent and saturable manner. In solution, SgI bound to spermatozoa in a non-Zn(2+)-dependent way, whereas immobilized SgI interacts with spermatozoa only in the presence of Zn(2+). It indicates that SgI must exhibit a specific structure or free flexibility to be able to interact with that ligand. Our results indicate that the association of SgI to spermatozoa is conformation dependent and specific. These findings could constitute a basis for the development of a male contraceptive.
Jonsson
Magnus
M
Department of Laboratory Medicine, Section for Clinical Chemistry, Entrance 71, Lund University, Malmö University Hospital, Malmö, Sweden. Magnus.Jonsson@med.lu.se
Frohm
Birgitta
B
Malm
Johan
J
D005434:Flow Cytometry; D006801:Humans; D008297:Male; D011485:Protein Binding; D012661:Semen / Q000737:chemistry / Q000378:metabolism; D029607:Seminal Vesicle Secretory Proteins / Q000737:chemistry / Q000378:metabolism*; D013094:Spermatozoa / Q000737:chemistry / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D015032:Zinc / Q000737:chemistry / Q000378:metabolism*
D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen; D015032:Zinc