A GA-insensitive dwarf mutant of Brassica napus L. correlated with mutation in pyrimidine box in the promoter of GID1.
A dwarf mutant from Brassica napus, namely NDF-1, which was derived from a high doubled haploid (DH) line '3529'(Brassica napus L.) of which seeds were jointly treated with chemical inducers and fast neutron bombardment, was revealed that dwarfism is under the control of a major gene(designated as ndf1) with a mainly additive effect and non-significant dominance effect. The germination and hypocotyls elongation response of dwarf mutants after exogenous GA and uniconazol application showed NDF-1 was a gibberellin insensitive dwarf. We cloned the Brassica napus GID1 gene, named BnGID1, and found it was the ortholog of AtGID1a. The sequence blasting of the BnGID1 genes from NDF-1 and wild type showed there was no mutant in the gene. But the quantitative RT-PCR analysis of GID1 EST pointed out the mutation was caused by the low-level expression of BnGID1 gene. After sequenced the BnGID1 gene's upstream, we found three bases mutated in the pyrimidine box (P-box) of the BnGID1 promoter, which is linkage with the dwarf mutant.
Li
Huapeng
H
Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, Sichuan, People's Republic of China. lhuapeng99@yahoo.cn
Wang
Yun
Y
Li
Xiaocheng
X
Gao
Yong
Y
Wang
Zhijun
Z
Zhao
Yun
Y
Wang
Maolin
M
D029681:Arabidopsis Proteins / Q000737:chemistry; D001483:Base Sequence; D029688:Brassica napus / Q000033:anatomy & histology* / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005875:Gibberellins / Q000494:pharmacology*; D005966:Glucuronidase / Q000378:metabolism; D018546:Hypocotyl / Q000187:drug effects / Q000254:growth & development; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011743:Pyrimidines / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; C512225:GID1a protein, Arabidopsis; D005875:Gibberellins; D010940:Plant Proteins; D011743:Pyrimidines; D011956:Receptors, Cell Surface; D005966:Glucuronidase