Isolation of bacteria from mouse caecal samples and description of Bacteroides sartorii sp. nov.
Caecal samples from wild-type and TNF(deltaARE) mice were cultured on selective media containing bile salts, amino acids or casein macro-peptides. Twenty-two strains were isolated and identified by 16S rRNA gene sequencing. Twenty-one strains showed >98% similarity to known bacteria (Blautia spp., Clostridium innocuum, Enterococcus spp., Escherichia coli, Lactobacillus murinus, Parabacteroides goldsteinii and Shigella dysenteriae). One additional isolate, strain A-C2-0, was a new bacterium. The closest relatives were Bacteroides massiliensis, Bacteroides dorei and Bacteroides vulgatus (< or = 94% similarity). Strain A-C2-0 is a Gram-negative rod that does not form spores and has a G + C content of DNA of 41.5%. Its major cellular fatty acid is C(15:0 ANTEISO), and its major respiratory quinone is MK-9. Cells are aerotolerant but grow only under strict anoxic conditions. They are resistant to cefotaxime and tobramycin. When compared with related Bacteroides spp., the new bacterium was positive for alpha-arabinosidase, negative for glutamyl glutamic acid arylamidase and did not metabolise galactose, glucose, fructose, mannose, raffinose and sucrose. Strain A-C2-0 therefore merits recognition as a member of a novel species within the genus Bacteroides, for which the name Bacteroides sartorii is proposed. The type strain is A-C2-0(T) (= DSM 21941(T) = CCUG 57211(T)).
Clavel
Thomas
T
Biofunctionality, ZIEL, Research Center for Nutrition and Food Science, Technische Universität München (TUM), Gregor-Mendel-Str. 2, 85350, Freising Weihenstephan, Germany. thomas.clavel@wzw.tum.de
Saalfrank
Anja
A
Charrier
Cédric
C
Haller
Dirk
D
D000818:Animals; D001439:Bacteroides / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D002432:Cecum / Q000382:microbiology*; D003470:Culture Media; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D020459:Genes, rRNA; D005838:Genotype; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D003470:Culture Media; D004269:DNA, Bacterial; D005227:Fatty Acids