title: Something worth dyeing for: molecular tools for the dissection of lipid metabolism in Caenorhabditis elegans.
abstract: The nematode Caenorhabditis elegans (C. elegans) has during the last decade emerged as an invaluable eukaryotic model organism to understand the metabolic and neuro-endocrine regulation of lipid accumulation. The fundamental pathways of food intake, digestion, metabolism, and signalling are evolutionary conserved between mammals and worms making C. elegans a genetically and metabolically extremely tractable model to decipher new regulatory mechanisms of lipid storage and to understand how nutritional and genetic perturbations can lead to obesity and other metabolic diseases. Besides providing an overview of the most important regulatory mechanisms of lipid accumulation in C. elegans, we also critically assess the current methodologies to monitor lipid storage and content as various methods differ in their applicability, consistency, and simplicity.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.
authors.forename: Ida Coordt
authors.initials: IC
authors.lastname: Elle
authors.forename: Louise Cathrine Braun
authors.initials: LC
authors.lastname: Olsen
authors.forename: Dennis
authors.initials: D
authors.lastname: Pultz
authors.forename: Steven Vestergaard
authors.initials: SV
authors.lastname: Rødkaer
authors.forename: Nils Joakim
authors.initials: NJ
authors.lastname: Faergeman
mesh_terms: D000818:Animals; D001692:Biological Transport; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D050356:Lipid Metabolism; D015398:Signal Transduction
chemical_list: D005456:Fluorescent Dyes