Rapid coomassie blue staining of protein gels.
Coomassie brilliant blue R250 (CBR-250) and silver staining are the most widely used methods for the routine visualization of proteins separated by SDS-PAGE. CBR-250 is an organic dye that complexes with basic amino acids, such as arginine, lysine, and histidine, as well as tyrosine. Conventional CBR-250 staining is capable of detecting as little as 30-100 ng of protein, but sensitivity can be improved by performing the staining and destaining at elevated temperatures. The method described in this protocol, which is a modified version of the conventional Coomassie protocol, speeds up the destaining process for faster results with increased sensitivity and is compatible with mass-spectrometry-based methods for identifying proteins.
Simpson
Richard J
RJ
D004396:Coloring Agents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012394:Rosaniline Dyes / Q000494:pharmacology*; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
D004396:Coloring Agents; D011506:Proteins; D012394:Rosaniline Dyes; C048139:Coomassie blue