[Study on IgG promoter as probe to evaluate safety of injections in pre-clinic].
OBJECTIVE
To construct an effective system screening and evaluating possible injections and components inducing allergy type II.


METHODS
Transfect IgG promoter-regulated green fluorescent protein expressing plasmid into RPMI-8226 cell. The number of fluorescent cells after drug treatment was calculated and statistically evaluated.


RESULTS
Tween 80 can suppress the expression of IgG effectively, and puerarin has activity of stimulating IgG expression, and the system has no response to KCl-treatment. No effect of Yuxingcao (Herba Houttuyniae) injection on this system is observed.


CONCLUSIONS
IgG promoter-drove green fluorescent protein expressing cell line can be used as system screening injections and components inducing allergy type II based on stimulating IgG promoter activity. Tween 80 can suppress the expression of IgG at the transcriptional level. Four batches of Yuxingcao injection cannot induce allergy type II by activating IgG expression.
Wang
Yugang
Y
Laboratory of Pharmaceutical Science, Department of Biological Sciences and Biotechnology, School of Medicine, Tsignghua University, Beijing 100084, China.
Li
Lele
L
Lei
Fan
F
Liang
Aihua
A
Xing
Dongming
D
Du
Lijun
L
D045744:Cell Line, Tumor; D015870:Gene Expression / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics*; D007267:Injections; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*
D007074:Immunoglobulin G; D010936:Plant Extracts; D049452:Green Fluorescent Proteins