title: Predominance of an ST11 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae clone causing bacteraemia and urinary tract infections in Korea.
abstract: To investigate the antimicrobial resistance, extended-spectrum beta-lactamases (ESBLs) and clones of Klebsiella pneumoniae isolates causing bacteraemia or urinary tract infection (UTI) in Korea, a total of 406 K. pneumoniae isolates from patients with bacteraemia (221 isolates) and UTI (185 isolates) were collected from 10 tertiary-care Korean hospitals from July 2006 to October 2007. In vitro antimicrobial susceptibility testing was performed for all isolates and ESBL production was tested. Multilocus sequence typing (MLST) analyses were performed to characterize genotypes of ESBL-producing K. pneumoniae isolates. PFGE was performed for sequence type 11 (ST11) isolates. Forty-seven UTI isolates (25.4 %) produced ESBLs, while 30 bacteraemia isolates (13.6 %) produced ESBLs (P=0.002). Among 77 ESBL-producing isolates, thirty-two (41.6 %) produced SHV-type ESBLs. bla(CTX-M) genes such as bla(CTX-M-14) and bla(CTX-M-15) were detected in 36.4 %. MLST and PFGE analyses showed that ST11 was dominant in ESBL-producing K. pneumoniae isolates causing UTI (57.4 %) and in those causing bacteraemia (70.0 %) and has been prevalent in Korean hospitals. ST11 isolates harbour a combination of different ESBL genes. The ST11 clone of ESBL-producing K. pneumoniae isolates prevails in Korea, but most isolates might acquire ESBL genes independently or several different clones might be distributed in Korea.
authors.affiliation: Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul 135-710, Republic of Korea.|Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Kwan Soo
authors.initials: KS
authors.lastname: Ko
authors.affiliation: Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul 135-710, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul 135-710, Republic of Korea.
authors.forename: Jin Yang
authors.initials: JY
authors.lastname: Baek
authors.affiliation: Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul 135-710, Republic of Korea.
authors.forename: Ji-Yoeun
authors.initials: JY
authors.lastname: Suh
authors.affiliation: Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul 135-710, Republic of Korea.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Lee
authors.affiliation: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Department of Internal Medicine, Kangbuk Samung Hospital, Sungkyunkwan University School of Medicine, Seoul 110-746, Republic of Korea.
authors.forename: Joon-Sup
authors.initials: JS
authors.lastname: Yeom
authors.affiliation: Chungnam National University Hospital, Daejeon 301-721, Republic of Korea.
authors.forename: Yeon-Sook
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Division of Infectious Diseases, Chonnam National University Medical School, Gwangju 501-757, Republic of Korea.
authors.forename: Sook-In
authors.initials: SI
authors.lastname: Jung
authors.affiliation: Jeju National University Hospital, Jeju National University School of Medicine, Jeju 690-716, Republic of Korea.
authors.forename: Sang Yop
authors.initials: SY
authors.lastname: Shin
authors.affiliation: Department of Internal Medicine, Gyeongsang National University School of Medicine and Gyeongsang Institute of Health Sciences, Jinju 660-702, Republic of Korea.
authors.forename: Sang Taek
authors.initials: ST
authors.lastname: Heo
authors.affiliation: Daegu Fatima Hospital, Daegu 701-600, Republic of Korea.
authors.forename: Ki Tae
authors.initials: KT
authors.lastname: Kwon
authors.affiliation: East-West Neo Medical Center, Kyunghee University, Seoul 134-727, Republic of Korea.
authors.forename: Jun Seong
authors.initials: JS
authors.lastname: Son
authors.affiliation: Kyungpook National University Hospital, Daegu 700-721, Republic of Korea.
authors.forename: Shin-Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Kyungpook National University Hospital, Daegu 700-721, Republic of Korea.
authors.forename: Hyun-Ha
authors.initials: HH
authors.lastname: Chang
authors.affiliation: Konkuk University Hospital, Seoul 143-729, Republic of Korea.
authors.forename: Hyun Kyun
authors.initials: HK
authors.lastname: Ki
authors.affiliation: Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Republic of Korea.
authors.forename: Doo Ryeon
authors.initials: DR
authors.lastname: Chung
authors.affiliation: Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Republic of Korea.
authors.forename: Kyong Ran
authors.initials: KR
authors.lastname: Peck
authors.affiliation: Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Republic of Korea.|Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul 135-710, Republic of Korea.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Song
mesh_terms: D016470:Bacteremia / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D007723:Korea / Q000453:epidemiology; D014552:Urinary Tract Infections / Q000453:epidemiology / Q000382:microbiology; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases