The effect of organic loading on bacterial community composition of membrane biofilms in a submerged polyvinyl chloride membrane bioreactor.
The effect of organic loading on bacterial community composition of membrane biofilms was investigated using a submerged polyvinyl chloride membrane bioreactor. The low and high loadings were set at 0.33 and 0.52 gCOD/(gVSSd), respectively. The results showed that membrane fouling occurred earlier and faster under the high loading conditions. Denaturing gradient gel electrophoresis (DGGE) analysis revealed that the similarity of bacterial community in the membrane biofilms between the two loadings was 0.67, higher than that in the mixed liquors (0.52-0.55), which indicated that some specific bacteria were selected preferentially on the membranes. Clone library analysis of the membrane biofilms indicated that Betaproteobacteria and Bacteroidetes under the high loading were 54.72% and 19.81%, respectively. Microarray results further confirmed that the two bacteria were the dominant microorganisms in the high loading biofilm. The severe membrane fouling may be aroused mainly by the enrichment of the two bacteria under the high loading.
Xia
Siqing
S
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. siqingxia@gmail.com
Li
Jixiang
J
He
Shuying
S
Xie
Kang
K
Wang
Xiaojia
X
Zhang
Yanhao
Y
Duan
Liang
L
Zhang
Zhiqiang
Z
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018441:Biofilms; D019149:Bioreactors; D004591:Electrophoresis, Polyacrylamide Gel; D008567:Membranes, Artificial; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011143:Polyvinyl Chloride; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D008567:Membranes, Artificial; D012336:RNA, Ribosomal, 16S; D011143:Polyvinyl Chloride