title: [Overexpression of adiponectin prevents hepatocyte steatosis].
abstract: OBJECTIVE
  To investigate the effect of adiponectin on hepatocyte steatosis.
  
  
  METHODS
  L02 cells were transfected with pEGFP-N1-AdipoQ, a plasmid encoding pEGFP-adiponectin fusion protein, or pEGFP-N1. Lipid droplets in the hepatocytes were observed by oil red staining at 72 h. The contents of TG, FFA and glycerol in hepatocytes were measured.
  
  
  RESULTS
  Compared to cells transfected with pEGFP-N1-AdipoQ plasmid, much more lipid droplets were observed in cells transfected with pEGFP-N1 plasmid. TG, FFA and glycerol contents in L02 cells and L02/pEGFP-N1 cells were significantly higher than those in L02/pEGFP-N1-AdipoQ cells.
  
  
  CONCLUSIONS
  Overexpression of adiponectin prevent hepatocyte steatosis.
authors.affiliation: Department of Clinincal, Chongqing Medical and Pharmaceutical College, Chongqing 400030, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Wen
authors.initials: W
authors.lastname: Sun
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.forename: Ting-Xiu
authors.initials: TX
authors.lastname: Xiang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiang
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Tao
authors.forename: Ai-Long
authors.initials: AL
authors.lastname: Huang
authors.forename: Pi-Long
authors.initials: PL
authors.lastname: Wang
mesh_terms: D052242:Adiponectin / Q000235:genetics*; D002460:Cell Line; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005234:Fatty Liver / Q000378:metabolism; D005822:Genetic Vectors; D005990:Glycerol / Q000032:analysis; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014162:Transfection; D014280:Triglycerides / Q000032:analysis
chemical_list: D052242:Adiponectin; D005230:Fatty Acids, Nonesterified; D011993:Recombinant Fusion Proteins; D014280:Triglycerides; D005990:Glycerol