Evaluation of subcutaneous and alveolar implantation surgical sites in the study of the biological properties of root-end filling endodontic materials.
OBJECTIVE
The aim of this study was to compare two methodologies used in the evaluation of tissue response to root-end filling materials in rats.


METHODS
Forty rats were divided into 4 groups: in Groups I and II (control groups), empty polyethylene tubes were implanted in the extraction site and in the subcutaneous tissue, respectively; in Groups III and IV, polyethylene tubes filled with ProRoot MTA were implanted in the extraction site and in the subcutaneous tissue, respectively. The animals were killed 7 and 30 days after tube implantation, and the hemi-maxillas and the capsular subcutaneous tissue, both with the tubes, were removed. Specimens were processed and evaluated histomorphologicaly under light microscopy. The scores obtained were analyzed statistically by the Kruskal-Wallis test (p<0.05).


RESULTS
There were no statistically significant differences between the implantation methods (p=0.78033, p=0.72039). It was observed that the 30-day groups presented a more mature healing process due to smaller number of inflammatory cells.


CONCLUSIONS
The present study showed no differences in tissue responses as far as the implantation site and the studied period were concerned. Alveolar socket implantation methodology represents an interesting method in the study of the biological properties of root-end filling endodontic materials due to the opportunity to evaluate bone tissue response.
Cintra
Luciano Tavares Angelo
LT
Department of Restorative Dentistry, Maringá Dental School, UNINGA University, Maringá, PR, Brazil. lucianocintra@hotmail.com.br
Bernabé
Pedro Felício Estrada
PF
de Moraes
Ivaldo Gomes
IG
Gomes-Filho
João Eduardo
JE
Okamoto
Tetuo
T
Consolaro
Alberto
A
Pinheiro
Tiago Novaes
TN
D017607:Aluminum Compounds / Q000494:pharmacology; D000539:Alveolar Process / Q000187:drug effects* / Q000473:pathology; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology; D003238:Connective Tissue / Q000473:pathology; D004338:Drug Combinations; D006097:Granulation Tissue / Q000473:pathology; D006171:Gutta-Percha / Q000494:pharmacology; D007249:Inflammation; D008422:Materials Testing; D018919:Neovascularization, Physiologic / Q000502:physiology; D010087:Oxides / Q000494:pharmacology; D020959:Polyethylene / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012184:Retrograde Obturation; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology; D040521:Subcutaneous Tissue / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors; D020390:Tooth Socket / Q000187:drug effects* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects
D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D006171:Gutta-Percha; D020959:Polyethylene