title: Tetrasomy 8 in a patient with chronic lymphocytic leukemia.
abstract: We report a case of a 47-year-old man diagnosed with chronic lymphocytic leukemia (CLL) with two extra copies of chromosome 8. Classical cytogenetic analysis by the immunostimulatory combination of DSP30 and interleukin 2 showed tetrasomy of chromosome 8 in 60% of the metaphase cells (48,XY,+8,+8[12]/46,XY[8]). Spectral karyotype analysis confirmed the abnormality previously seen by G banding. Additionally, interphase fluorescence in situ hybridization using an LSI CEP 8 probe performed on peripheral blood cells without any stimulant agent showed tetrasomy of chromosome 8 in 54% of analyzed cells (108 of 200). To our knowledge, tetrasomy 8 as the sole chromosomal abnormality in CLL has not been previously described. The prognostic significance of tetrasomy 8 in CLL remains to be elucidated. However, the patient has been followed up in the outpatient hospital since 2004 without any therapeutic intervention and has so far remained stable.
authors.affiliation: Department of Internal Medicine, Division of Hematology, School of Medicine of Ribeirão Preto, University of São Paulo, 14049-900 Ribeirão Preto, SP, Brazil. fabiomorato@usp.br
authors.forename: Fábio Morato
authors.initials: FM
authors.lastname: de Oliveira
authors.forename: Renata Amorim
authors.initials: RA
authors.lastname: Brandão
authors.forename: Sabrina Dias
authors.initials: SD
authors.lastname: Leite-Cueva
authors.forename: Francisco
authors.initials: F
authors.lastname: de Paula Careta
authors.forename: Belinda Pinto
authors.initials: BP
authors.lastname: Simões
authors.forename: Eduardo Magalhães
authors.initials: EM
authors.lastname: Rego
authors.forename: Roberto Passetto
authors.initials: RP
authors.lastname: Falcão
mesh_terms: D002869:Chromosome Aberrations; D002898:Chromosomes, Human, Pair 8; D020732:Cytogenetic Analysis; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011123:Polyploidy