Three-dimensional characterization of active membrane waves on living cells.
We measure the temporal evolution of three-dimensional membrane topography on living fibroblasts and characterize the propagation of membrane waves using a wide-field optical profiling technique. The measured membrane profiles are compared with the numerical results calculated by the active membrane model recently proposed by Shlomovitz and Gov. After the treatments of blebbistatin and latrunculin A separately, the membrane waves disappear and the membrane surfaces are flattened, verifying that the membrane waves are driven by the interactions between myosin II and actin polymerization.
Chen
Chien-Hong
CH
Institute of Biophotonics, National Yang-Ming University, 155 Sec. 2, Linong Street, Taipei 11221, Taiwan.
Tsai
Feng-Ching
FC
Wang
Chun-Chieh
CC
Lee
Chau-Hwang
CH
D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D008853:Microscopy / Q000379:methods*; D053778:Thiazolidines / Q000494:pharmacology; D013997:Time Factors
D019086:Bridged Bicyclo Compounds, Heterocyclic; D006576:Heterocyclic Compounds, 4 or More Rings; D053778:Thiazolidines; C472645:blebbistatin; C037067:latrunculin A