title: Selective breast cancer cell capture, culture, and immunocytochemical analysis using self-assembled magnetic bead patterns in a microfluidic chip.
abstract: Separation and subsequent culturing of MCF-7 breast cancer cells on self-assembled protein-coated magnetic beads in a microfluidic chip is demonstrated. The beads were patterned in situ inside a sealed microfluidic channel using magnetic-field-assisted electrostatic self-assembly. Hereafter, they were grafted by exposure to a solution of 5D10 monoclonal antibodies (mAb) and fibronectin (FN), with the first being used for immunospecific cell capture and the latter being used for cell adhesion and growth. A solution of target MCF-7 cells mixed with Jurkat cells was brought inside the microchannel, leading to specific MCF-7 cell capture; the latter were then cultured and evidenced by cell immuno-luminescence.
authors.affiliation: Laboratory of Microsystems, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland. venkataragavalu.sivagnanam@epfl.ch
authors.forename: Venkataragavalu
authors.initials: V
authors.lastname: Sivagnanam
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Caroline
authors.initials: C
authors.lastname: Vandevyver
authors.forename: Jean-Claude G
authors.initials: JC
authors.lastname: Bünzli
authors.forename: Martin A M
authors.initials: MA
authors.lastname: Gijs
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002469:Cell Separation / Q000295:instrumentation*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D019169:Jurkat Cells; D008163:Luminescent Measurements; D008280:Magnetics; D046210:Microfluidic Analytical Techniques; D008863:Microspheres
chemical_list: D015415:Biomarkers