title: A supramolecular bifunctional artificial enzyme with superoxide dismutase and glutathione peroxidase activities.
abstract: For constructing a bifunctional antioxidative enzyme with both superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, a supramolecular artificial enzyme was successfully constructed by the self-assembly of the Mn(III)meso-tetra[1-(1-adamantyl methyl ketone)-4-pyridyl] porphyrin (MnTPyP-M-Ad) and cyclodextrin-based telluronic acid (2-CD-TeO(3)H) through host-guest interaction in aqueous solution. The self-assembly of the adamantyl moieties of Mn(III) porphyrin and the beta-CD cavities of 2-CD-TeO(3)H was demonstrated by the NMR spectra. In this supramolecular enzyme model, the Mn(III) porphyrin center acted as an efficient active site of SOD and tellurol moiety endowed GPx activity. The SOD-like activity (IC(50)) of the new catalyst was found to be 0.116 microM and equals to 2.56% of the activity of the native SOD. Besides this, supramolecular enzyme model also showed a high GPx activity, and a remarkable rate enhancement of 27-fold compared to the well-known GPx mimic ebselen was observed. More importantly, the supramolecular artificial enzyme showed good thermal stability.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Shuangjiang
authors.initials: S
authors.lastname: Yu
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Lu
authors.initials: L
authors.lastname: Miao
authors.forename: Junyan
authors.initials: J
authors.lastname: Zhu
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Yin
authors.forename: Quan
authors.initials: Q
authors.lastname: Luo
authors.forename: Jiayu
authors.initials: J
authors.lastname: Xu
authors.forename: Jiacong
authors.initials: J
authors.lastname: Shen
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
mesh_terms: D000218:Adamantane / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D020134:Catalytic Domain; D005979:Glutathione Peroxidase / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008345:Manganese / Q000737:chemistry / Q000378:metabolism; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D047392:beta-Cyclodextrins / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011166:Porphyrins; D047392:beta-Cyclodextrins; D008345:Manganese; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C031215:betadex; D000218:Adamantane