[Studies on IFN-gamma-mediated reversion of CIK killing sensitivity to edited human lung cancer A549 cells].
OBJECTIVE
To explore IFN-gamma-mediated reversion of CIK killing sensitivity to immunoedited lung cancer A549 cells.


METHODS
RT-PCR and MTT methods were used to detect the effect on MICA mRNA expression induced by IFN-gamma in the edited A549 cells and the change of CIK killing sensitivity to A549 cells, respectively.


RESULTS
Low expression of cell surface MICA and low killing sensitivity of CIKs were observed in edited A549 cells. IFN-gamma could significantly increase MICA mRNA expression in the edited A549 cells and improve CIK cell cytotoxicity.


CONCLUSIONS
IFN-gamma could reverse CIK killing sensitivity to the edited A549 cells by enhancing the MICA mRNA expression.
Liu
Ji-fang
JF
Laboratory Center of the Affiliated Tumor Hospital of Guangzhou Medical College, Guangzhou 510095, China. jifangliu@hotmail.com
Liu
Feng
F
Qi
Yan-chao
YC
Yang
Bo
B
Lu
Min-ying
MY
Pan
Dong-xiao
DX
Shen
Hong-zhuo
HZ
D045744:Cell Line, Tumor; D055612:Cytokine-Induced Killer Cells / Q000187:drug effects* / Q000276:immunology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000473:pathology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; D012333:RNA, Messenger; D007371:Interferon-gamma