title: Meat quality and carcass traits in relation to HGD-BstXI and HGD-HaeIII PCR-RFLP polymorphism in Chinese red cattle.
abstract: We investigated the effect of homogentisate 1, 2 dioxygenase (HGD) gene on meat quality and carcass traits in 287 Chinese red cattle. The PCR-SSCP method was used to identify polymorphism of the HGD gene in the exon 1 and intron 1. Two polymorphisms were detected in intron 1 and two restriction sites for endonuclease HGD-BstXI and HGD-HaeIII have also been found. The HGD-BstXI genotypes showed significant effects on cooking loss, drip loss, net meat weight, carcass weight, and eye muscle area (P<0.05). The HGD-HaeIII genotypes significant affected cooking loss, muscle fibre diameter, shear force, drip loss, and carcass yield ratio (P<0.05). Moreover, we found significant effects of diplotypes on cooking loss, muscle fibre diameter, shear force, drip loss, net meat weight, carcass weight, and eye muscle area (P<0.05).
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, People's Republic of China; College of Life Science, Liaocheng University, Liaocheng 252059, People's Republic of China.
authors.forename: G L
authors.initials: GL
authors.lastname: Zhou
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: L C
authors.initials: LC
authors.lastname: Zhang
authors.forename: Y S
authors.initials: YS
authors.lastname: Yu
authors.forename: H G
authors.initials: HG
authors.lastname: Jin
mesh_terms: D000818:Animals; D001823:Body Composition / Q000235:genetics / Q000502:physiology*; D002417:Cattle / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D050560:Homogentisate 1,2-Dioxygenase / Q000235:genetics*; D008460:Meat / Q000592:standards*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D050560:Homogentisate 1,2-Dioxygenase; C047564:CCANNNNNNTGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases