[The diagnostic value of fluorescence quantitative PCR in screening of influenza A virus].
OBJECTIVE
To discuss the the diagnostic value of fluorescence quantitative PCR in screening of influenza A virus.


METHODS
Influenza A virus RNA in 150 cases of suspected influenza patients with throat swab was measured by fluorescence quantitative RT-PCR. The white blood cell count was measured by XT-1800 automated hematology analyzer in patients' anticoagulant EDTA whole blood. 45 cases of influenza A virus RNA-positive patients were detected with influenza A virus core protein by colloidal gold.


RESULTS
In 150 patients, 54 cases were detected influenza A virus RNA positive, the positive rate was 36%.The 54 cases of influenza A virus RNA-positive patients were detected with influenza A virus core protein by colloidal gold, the results were all negative.The white blood cell count is (6.81+/-2.12) x 10(9)/L in the influenza A virus RNA-positive patients, and (6.64+/-3.13) x 10(9)/L for the negative cases. White blood cell count in patients with influenza A and non-influenza patients have no significant difference.


CONCLUSIONS
Fluorescence quantitative RT-PCR in the detection of influenza A virus RNA has a better positive rate, its sensitivity and specificity are better than colloidal gold and white blood cell count, and it can be screened quickly and efficiently in patients with influenza to prevent disease outbreaks.
Shi
Yu-ling
YL
Department of Clinical Laboratory, General Hospital of Guangzhou Command, Guangzhou 510010, China. shiyuling6@sina.com
Yang
Yong-quan
YQ
Zeng
Lan-lan
LL
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000097:blood / Q000175:diagnosis*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis; D013050:Spectrometry, Fluorescence; D014758:Viral Core Proteins / Q000032:analysis; D055815:Young Adult
D012367:RNA, Viral; D014758:Viral Core Proteins