Trinity DNA detection platform by ultrasmooth and functionalized PEDOT biointerfaces.
An oligonucleotide-grafted poly(3,4-ethylenedioxythiophene) (PEDOT) thin film is developed for three DNA biosensor detection methods, including fluorescence, quartz crystal microbalance, and electrochemical methods. By electrocopolymerization of hydroxyl-functionalized EDOT and carboxylic-functionalized EDOT in microemulsion solutions, ultrasmooth films with a controlled surface density of carboxylic groups are created. The probe oligonucleotides are immobilized on PEDOT thin films by using a N-hydroxysuccinimide and 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide hydrochloride coupling method. By monitoring the DNA hybridization efficiency on thin films with different oligonucleotide densities, the optimized density for DNA hybridization is obtained. The feasibility and limitation of using this platform for electrochemical detection are also discussed.
Luo
Shyh-Chyang
SC
Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore 138669, Singapore.
Xie
Hong
H
Chen
Naiyan
N
Yu
Hsiao-Hua
HH
D015374:Biosensing Techniques; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004553:Electric Conductivity; D004563:Electrochemistry / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008956:Models, Chemical; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
D019086:Bridged Bicyclo Compounds, Heterocyclic; D009841:Oligonucleotides; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D004247:DNA