title: Chemical and in vitro study of the potential of 3-(aryl)-2-sulfanylpropenoic acids and their Zn(II) complexes as protective agents against cadmium toxicity.
abstract: The free H(2)xspa ligands [xspa = pspa, Clpspa, tspa or fspa where p = 3-(phenyl), Clp = 3-(2-chlorophenyl), t = 3-(2-thienyl), f = 3-(2-furyl) and spa = 2-sulfanylpropenoato], their Zn(II) complexes of formula [HQ](2)[Zn(xspa)(2)] (HQ = diisopropylammonium) and the Cd(II) equivalents were prepared and characterized by elemental analysis and by IR, Raman and NMR ((1)H, (13)C) spectroscopy. X-Ray studies of the crystal structures of [HQ](2)[Zn(pspa)(2)], [HQ](2)[Zn(Clpspa)(2)], [HQ](2)[Zn(tspa)(2)] and [HQ](2)[Zn(fspa)(2)] show that the zinc atom is coordinated to two O atoms and two S atoms of the ligands in a distorted tetrahedral ZnO(2)S(2) environment. In the structures of [HQ](2)[Cd(pspa)(2)] and [HQ](2)[Cd(Clpspa)(2)] the cadmium atom is coordinated to three S atoms and two carboxylato O atoms of the ligands in a distorted trigonal bipyramidal environment. The interchange of ligands between Zn(II) and Cd(II) was studied by (113)Cd NMR spectroscopy. The in vitro protective effect of H(2)xspa and their Zn(II) complexes against Cd toxicity was investigated using the human hepatocarcinoma HepG2 cell line and the pig renal proximal tubule LLC-PK1 cell line. The incorporation of Zn(II) was found to be relevant in the case of H(2)pspa, with an increase observed in the cell viability of the LCC-PK1 cells with respect to the value for the free ligand.
authors.affiliation: Departamento de Química Inorgánica, Facultade de Farmacia, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Galicia, Spain.
authors.forename: J S
authors.initials: JS
authors.lastname: Casas
authors.forename: E E
authors.initials: EE
authors.lastname: Castellano
authors.forename: M D
authors.initials: MD
authors.lastname: Couce
authors.forename: M
authors.initials: M
authors.lastname: García-Vega
authors.forename: A
authors.initials: A
authors.lastname: Sánchez
authors.forename: A
authors.initials: A
authors.lastname: Sánchez-González
authors.forename: J
authors.initials: J
authors.lastname: Sordo
authors.forename: J M
authors.initials: JM
authors.lastname: Varela
authors.forename: E M
authors.initials: EM
authors.lastname: Vázquez López
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D002460:Cell Line; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D018360:Crystallography, X-Ray; D056945:Hep G2 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D020011:Protective Agents / Q000138:chemical synthesis / Q000737:chemistry*; D013552:Swine; D015032:Zinc / Q000737:chemistry*
chemical_list: D000179:Acrylates; D056831:Coordination Complexes; D020011:Protective Agents; D002104:Cadmium; D015032:Zinc; C036658:acrylic acid