Clonality and phenotyping of canine lymphomas before chemotherapy and during remission using polymerase chain reaction (PCR) on lymph node cytologic smears and peripheral blood.
Polymerase chain reaction (PCR) assays for the immunoglobulin and T-cell receptor genes were utilized to determine phenotype and clonality from lymph node cytologic smears and peripheral blood lymphocytes from 10 dogs with lymphoma, before chemotherapy and during remission. Results were compared with those from 13 dogs with a cytologic diagnosis of lymph node hyperplasia. Clonality was identified in 7 of the lymphomas on the basis of either lymph node cytology or peripheral blood lymphocytes before treatment. No lymph node hyperplasia samples were clonal. In 6 of the dogs with lymphoma, clonality was demonstrated during clinical remission. Detection of PCR clonality during clinical remission is an effective means of identifying minimal residual disease in canine lymphoma and thus additional work is warranted to determine if molecular remission is prognostic or predictive for outcome in well-controlled and well-defined lymphoma subtypes.
Thilakaratne
Dilini N
DN
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4.
Mayer
Monique N
MN
MacDonald
Valerie S
VS
Jackson
Marion L
ML
Trask
Brenda R
BR
Kidney
Beverly A
BA
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D002999:Clone Cells / Q000737:chemistry / Q000473:pathology*; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D004283:Dog Diseases / Q000188:drug therapy / Q000473:pathology*; D004285:Dogs; D006965:Hyperplasia / Q000473:pathology / Q000662:veterinary; D008198:Lymph Nodes / Q000737:chemistry / Q000166:cytology* / Q000473:pathology; D008223:Lymphoma / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D018365:Neoplasm, Residual / Q000662:veterinary; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011379:Prognosis; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012074:Remission Induction
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D011948:Receptors, Antigen, T-Cell