Diazepam fluorimetric monitoring upon photo-degradation in an automatic miniaturized flow system.
The present work describes the fully integration in line of a photo-degradation unit, comprising a low pressure UV lamp, in a Multipumping Flow System (MPFS), for the fluorimetric chemical control of commercially available pharmaceutical formulations containing diazepam. The utilization of an organized micellar medium provided enhanced fluorescence emission. The results allowed to obtain a linear working range for diazepam concentrations of up to 40 mg L(-1) (r = 0.9998) and the detection limit was about 0.97 mg L(-1). The results obtained by the miniaturized and automatic flow system were in agreement with those furnished by the reference procedure, with relative deviations comprised between -2.09% and 2.13%.
Ribeiro
David S M
DS
REQUIMTE, Department of Physical-Chemistry, Faculty of Pharmacy, University of Porto, Rua Aníbal Cunha 164, 4050-047, Porto, Portugal.
Prior
João A V
JA
Santos
João L M
JL
Lima
José L F C
JL
D001331:Automation; D002626:Chemistry, Pharmaceutical; D003975:Diazepam / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods*; D008904:Miniaturization / Q000379:methods*; D055668:Photochemical Processes; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
D003975:Diazepam