title: Electrochemical recognition for sugars on the chitosan-poly(diallyldimethylammonium chloride)-nano-Prussian blue/nano-Au/4-mercaptophenylboronic acid modified glassy carbon electrode.
abstract: A new amperometric biosensor for the detection of sugars was prepared. A glassy carbon electrode was modified with Prussian blue (PB) nanoparticles protected by chitosan (CS) and poly(diallyldimethylammonium chloride) (PDDA), and then gold nanoparticles were assembled onto the electrode followed by the assembly of 4-mercaptophenylboronic acid (MPBA) onto the surface of gold nanoparticles through a sulfur-Au bond to fabricate a self-assembled biosensor. The PB nanoparticles protected by CS and PDDA were characterized using transmission electron microscopy and UV-vis absorption spectroscopy. The characterization of the self-assembled electrode was investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The pKa values of the MPBA monolayer before and after combining with sugars were determined. The fabricated electrode exhibited excellent performances for determining D(+)-glucose, D(+)-mannose, and D(-)-fructose on the basis of the change in i(p) of the Fe(CN)6(3-/4-) ion in the presence of sugars.
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, People's Republic of China.
authors.forename: Xiu Zhu
authors.initials: XZ
authors.lastname: Bian
authors.forename: Hong Qun
authors.initials: HQ
authors.lastname: Luo
authors.forename: Nian Bing
authors.initials: NB
authors.lastname: Li
mesh_terms: D000498:Allyl Compounds / Q000737:chemistry*; D001897:Boronic Acids / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis*; D002244:Carbon; D048271:Chitosan / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry*; D036103:Nanotechnology; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: C510906:4-mercaptophenylboronic acid; D000498:Allyl Compounds; D001897:Boronic Acids; D002241:Carbohydrates; D005295:Ferrocyanides; D000644:Quaternary Ammonium Compounds; D013438:Sulfhydryl Compounds; D002244:Carbon; C508190:diallyldimethylammonium chloride; D048271:Chitosan; C000170:ferric ferrocyanide