Biodegradable nano-polymeric system for efficient Akt1 siRNA delivery.
Biodegradable nano-polymeric carrier composed of polycaprolactone (PCL) and polyethylenimine (PEI) (BNPP) was successfully synthesized for the delivery of sh/siRNA in lung cancer cells. BNPP efficiently and safely delivered siRNA in lung cancer cells. BNPP-delivered Akt1 siRNA silenced Akt1 protein, and reduced the cancer cell survival, proliferation, malignancy and metastasis.
Jere
Dhananjay
D
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea.
Arote
Rohidas
R
Jiang
Hu-Lin
HL
Kim
You-Kyoung
YK
Cho
Myung-Haing
MH
Cho
Chong-Su
CS
D020341:Absorbable Implants; D045744:Cell Line, Tumor; D020868:Gene Silencing; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics*; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D014162:Transfection / Q000379:methods
D053769:Nanocapsules; D011108:Polymers; D034741:RNA, Small Interfering; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt