title: Enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture.
abstract: In fed-batch culture of Klebsiella pneumoniae, 1,3-propanediol production was growth associated, while the by-products, including lactic acid and ethanol, increased sharply as the cells grew slowly. When the fed-batch culture was supplied with a mixture of organic acids including citrate, fumarate and succinate, cell growth and 1,3-propanediol production increased significantly, whereas the by-products, especially lactic acid and ethanol, decreased sharply. High concentrations of PDO and acetate inhibited cell growth and PDO production. To improve the PDO production, repeated fed-batch culture with addition of the organic acid mixture was performed in a 5-l reactor. The fed-batch culture was repeated five times, and the 1,3-propanediol yield and concentration reached above 0.61 mol mol(-1) and 66 g l(-1), respectively, in 20 h for each cycle. Furthermore, the PDO productivity reached above 3.30 g l(-1) h(-1) in each cycle, which was much higher than that of the original fed-batch culture.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Xue
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Li
authors.forename: Yuelan
authors.initials: Y
authors.lastname: Xia
authors.forename: Qin
authors.initials: Q
authors.lastname: Ye
mesh_terms: D000144:Acids, Acyclic / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D046508:Culture Techniques; D005285:Fermentation; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000378:metabolism*; D011409:Propylene Glycols / Q000378:metabolism*
chemical_list: D000144:Acids, Acyclic; D011409:Propylene Glycols; C041787:1,3-propanediol