title: Identification of molecularly defined Staphylococcus aureus strains using matrix-assisted laser desorption/ionization time of flight mass spectrometry and the Biotyper 2.0 database.
abstract: Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has recently been introduced for bacterial identification. To our knowledge, this is the first study where the Biotyper 2.0 database (Bruker Daltonics) has been applied for bacterial identification in a local strain collection of molecularly defined Staphylococcus aureus. We showed that the accuracy of the Biotyper 2.0-based identification for 602 molecularly defined strains of S. aureus, irrespective of meticillin resistance, was equivalent to that of the molecularly defined reference even at a score cut-off value of 2. Also, 412 isolates of 20 different species of non-S. aureus staphylococci were all correctly identified to species level compared to the molecularly defined reference. Moreover, the MALDI-TOF MS-based S. aureus identification approach was clearly faster than more time-consuming methods such as a molecular identification approach.
authors.affiliation: Institute for Hygiene and Microbiology, Department of Medical Microbiology, University of Bochum, Bochum, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Szabados
authors.affiliation: Institute for Hygiene and Microbiology, Department of Medical Microbiology, University of Bochum, Bochum, Germany.
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Woloszyn
authors.affiliation: Institute for Hygiene and Microbiology, Department of Medical Microbiology, University of Bochum, Bochum, Germany.
authors.forename: Cindy
authors.initials: C
authors.lastname: Richter
authors.affiliation: Institute for Hygiene and Microbiology, Department of Medical Microbiology, University of Bochum, Bochum, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Kaase
authors.affiliation: Institute for Hygiene and Microbiology, Department of Medical Microbiology, University of Bochum, Bochum, Germany.
authors.forename: Sören
authors.initials: S
authors.lastname: Gatermann
mesh_terms: D016208:Databases, Factual; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics*