title: Single-step affinity purification for fungal proteomics.
abstract: A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches, which should be widely applicable, are included.
authors.affiliation: Department of Molecular Genetics, Ohio State University, 496 W. 12th Ave., Columbus, OH 43210, USA.
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Liu
authors.forename: Aysha H
authors.initials: AH
authors.lastname: Osmani
authors.forename: Leena
authors.initials: L
authors.lastname: Ukil
authors.forename: Sunghun
authors.initials: S
authors.lastname: Son
authors.forename: Sarine
authors.initials: S
authors.lastname: Markossian
authors.forename: Kuo-Fang
authors.initials: KF
authors.lastname: Shen
authors.forename: Meera
authors.initials: M
authors.lastname: Govindaraghavan
authors.forename: Archana
authors.initials: A
authors.lastname: Varadaraj
authors.forename: Shahr B
authors.initials: SB
authors.lastname: Hashmi
authors.forename: Colin P
authors.initials: CP
authors.lastname: De Souza
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Osmani
mesh_terms: D001233:Aspergillus nidulans / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005656:Fungal Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D005656:Fungal Proteins