Human mesenchymal stem cell transplantation protects against cerebral ischemic injury and upregulates interleukin-10 expression in Macacafascicularis.
Mesenchymal stem cell (MSC) transplantation has been reported to improve neurologic function after ischemic injury. However, the detailed mechanisms by which MSCs promote functional recovery are not fully understood. Interleukin-10 (IL-10) is a well-known anti-inflammatory cytokine with neuroprotective effects with respect to brain injury. In this study, a non-human primate ischemia model was used to test the hypothesis that transplanted human bone-marrow-derived MSCs (hBMSCs) exert a neuroprotective effects on cerebral ischemia and upregulate IL-10 expression. We also assessed neuronal apoptosis and astroglial activity in the area around the ischemic lesion and proliferating cells in the subventricular zone (SVZ). Results showed that hBMSC transplantation in ischemic tissues improved the neurological functions and induced an increase in IL-10 expression. In addition, neuronal apoptosis and astroglial activity in the peri-ischemic area decreased, and the number of proliferating cells in the SVZ increased. These results provide a novel therapeutic strategy for improving neurologic function after cerebral ischemia.
Li
Jiamei
J
Institute of Laboratory Animal Science, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100021, PR China.
Zhu
Hua
H
Liu
Ying
Y
Li
Qin
Q
Lu
Shan
S
Feng
Ming
M
Xu
Yanfeng
Y
Huang
Lan
L
Ma
Chunmei
C
An
Yihua
Y
Zhao
Robert Chunhua
RC
Wang
Renzhi
R
Qin
Chuan
C
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D020520:Brain Infarction / Q000209:etiology / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D002545:Brain Ischemia / Q000150:complications; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020287:In Situ Nick-End Labeling / Q000379:methods; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D008252:Macaca fascicularis; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009460:Neurologic Examination / Q000379:methods; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012395:Rose Bengal; D015854:Up-Regulation / Q000502:physiology*
D005904:Glial Fibrillary Acidic Protein; D018809:Proliferating Cell Nuclear Antigen; D016753:Interleukin-10; D012395:Rose Bengal