Biopolymer-modified graphite oxide nanocomposite films based on benzalkonium chloride-heparin intercalated in graphite oxide.
Heparin is a potent anticoagulant agent that interacts strongly with antithrombin III to prevent the formation of fibrin clots. In the present work, poly(dimethylsiloxane)(PDMS)/graphite oxide-benzalkonium chloride-heparin (PDMS/modified graphite oxide) nanocomposite films were obtained by the solution intercalation technique as a possible drug delivery system. The heparin-benzalkonium chloride (BAC-HEP) was intercalated into graphite oxide (GO) layers to form GO-BAC-HEP (modified graphite oxide). Nanocomposite films were characterized by XRD, SEM, TEM, ATR-FTIR and TGA. The modified graphite oxide was observed to be homogeneously dispersed throughout the PDMS matrix. The effect of modified graphite oxide on the mechanical properties of the nanocomposite film was investigated. When the modified graphite oxide content was lower than 0.2 wt%, the nanocomposites showed excellent mechanical properties. Furthermore, nanocomposite films become delivery systems that release heparin slowly to make the nanocomposite films blood compatible. The in vitro studies included hemocompatibility testing for effects on platelet adhesion, platelet activation, plasma recalcification profiles, and hemolysis. Results from these studies showed that the anticoagulation properties of PDMS/GO-BCA-HEP nanocomposite films were greatly superior to those for no treated PDMS. Cell culture assay indicated that PDMS/GO-BCA-HEP nanocomposite films showed enhanced cell adhesion.
Meng
Na
N
Jiangsu Engineering Research Center for Biomedical Function Materials, Nanjing Normal University, Nanjing 210046, People's Republic of China.
Zhang
Shuang-Quan
SQ
Zhou
Ning-Lin
NL
Shen
Jian
J
D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology; D001548:Benzalkonium Compounds / Q000737:chemistry*; D001704:Biopolymers / Q000737:chemistry; D001777:Blood Coagulation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006461:Hemolysis; D006493:Heparin / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D019007:P-Selectin / Q000378:metabolism; D010973:Platelet Adhesiveness / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction
D000925:Anticoagulants; D001548:Benzalkonium Compounds; D001704:Biopolymers; D004129:Dimethylpolysiloxanes; D009757:Nylons; D010087:Oxides; D019007:P-Selectin; C085707:poly(dimethylsiloxane)-polyamide copolymer; D006108:Graphite; D006493:Heparin