Expressions of SE-1, CD31 and CD105 in the vascular endothelial cells and serum of rat with hepatocellular carcinoma.
BACKGROUND
Hepatocellular carcinoma (HCC) is one of the deadliest cancers worldwide. In order to investigate the molecular biologic mechanism of HCC's development, we studied the expressions of SE-1, CD105 and CD31 in tumor endothelial cells (TECs) of HCC and in the serum of rats.


METHODS
We analyzed the expressions of SE-1, CD31 and CD105 in rat HCC tumor tissues using immunohistochemistry (IHC). Twenty HCC bearing rats and eighteen normal rats were examined for the expressions of SE-1, CD31 and CD105 antigens in serum by enzyme-linked immunosorbent assay (ELISA).


RESULTS
SE-1, CD31 and CD105 antigens were detected both in HCC tissue and in normal liver tissue with higher expressions of CD31 and CD105 in HCC while the SE-1 antigen expression was higher in normal liver. Similarly, serum CD31 and CD105 in rats with HCC were significantly increased compared with normal rats (t = 2.8628, P = 0.0086; t = 4.4922, P < 0.0001, respectively). In contrast, SE-1 antigen in HCC rat serum was significantly decreased compared with normal rats (t = 3.4983, P = 0.0011).


CONCLUSIONS
SE-1, CD31 and CD105 are closely related with liver tumor angiogenesis, which is similar to their performances in terms of their expressions in the serum.
Wang
Jing-yu
JY
Department of Infectious Disease, Peking University First Hospital, Beijing 100034, China.
Xu
Xiao-yuan
XY
Jia
Jing-hui
JH
Wu
Chi-hong
CH
Ge
Ruo-wen
RW
D000818:Animals; D015703:Antigens, CD / Q000097:blood*; D006528:Carcinoma, Hepatocellular / Q000098:blood supply / Q000737:chemistry*; D042783:Endothelial Cells / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D008114:Liver Neoplasms, Experimental / Q000098:blood supply / Q000737:chemistry*; D008297:Male; D009389:Neovascularization, Pathologic / Q000097:blood; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000097:blood*; D051381:Rats; D011915:Rats, Inbred BUF
D015703:Antigens, CD; D019408:Platelet Endothelial Cell Adhesion Molecule-1