[Impact of salvianolic acid-B on TGF-beta1-induced HK-2 epithelial-mesenchymal transition].
OBJECTIVE
To investigate the effect of salvianolic-acid B (SA-B) on epithelia-mesenchymal transition in human renal proximal tubular cells (HK2), induced by transforming growth factor beta1 (TGF-beta1).


METHODS
Epithelia-mesenchymal transition (EMT) was induced with TGF-beta1 in HK2 cultured in vitro. Different concentrations (2, 5, 10, 20 microg x L(-1)) and stimulant periods (12, 24, 48 h) were tried to find the perfect condition for EMT. At the same time bone morphogenetic protein-7 (BMP-7, positive control) and the SA-B intervention were given to observe their effect on EMT. Western blot and immunofluorescent microscopy were used to analyze the expression of E-cadherin and alpha-smooth muscle actin (alpha-SMA) in HK2.


RESULTS
BMP-7 significantly inhibited the down-regulation of E-cadherin and the up-regulation of alpha-SMA induced by TGF-beta1 (P < 0.05), and SA-B significantly inhibited the up-regulation of alpha-SMA expression induced by TGF-beta1 (P < 0.05), but not the down-regulation of E-cadherin induced by TGF-beta1.


CONCLUSIONS
SA-B and BMP-7 can inhibit TGF-beta1-induced EMT in HK2. Their common role is to inhibit the up-regulation of alpha-SMA, and the effect of SA-B on the regulation of E-cadherin needs further study to be confirmed.
Zhou
Juan
J
Scientific Experimental Center of Shanghai Traditional Chinese Medicine University, Shanghai 201203, China.
Wang
Fei
F
Lu
Haiying
H
Zhang
Yue
Y
D001572:Benzofurans / Q000494:pharmacology*; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology; D015820:Cadherins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D004847:Epithelial Cells / Q000473:pathology*; D006801:Humans; D008648:Mesoderm / Q000473:pathology*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D053630:Depsides
D001572:Benzofurans; D055419:Bone Morphogenetic Protein 7; D015820:Cadherins; D053773:Transforming Growth Factor beta1; C076944:salvianolic acid B; D053630:Depsides