[Determination of fenbutatin oxide residue in orange products by gas chromatography].
An analytical method for the determination of fenbutatin oxide (FBT) residue in oranges by capillary gas chromatography-flame photometric detection (GC-FPD) was developed. The FBT was extracted with acetone-acetic acid (99:1, v/v) and hexane, filtered and evaporated by nitrogen evaporator in a water bath at 35 degrees C. The residue was dissolved in hexane. The FBT in the solvent was derivatized with ethyl magnesium bromide for 15 min, 1 mol/L hydrochloride was added, the supernatant was collected and the solvent was evaporated to get dry supernatants, then the supernatant was dissolved in hexane and cleaned up with a silica solid phase extraction column, eluted with 5 mL hexane-dichloromethane (4:1, v/v), determined by GC. The standard curve was linear in the range of 0.2-2.0 mg/L. The correlation coefficients (r) were more than 0. 999 5, the average recoveries were 79.6%-109.6% with the relative standard deviations (RSDs) of 3.60%-9.04% at the spiked levels of 0.1-0.4 mg/kg, and the detection limit of fenbutatin oxide was 0.1 mg/kg. This method is suitable for the analysis of fenbutatin oxide residue in orange products.
Liu
Zhixing
Z
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Guo
Ping
P
Wang
Yuanxing
Y
Zhan
Chunrui
C
Zuo
Haigen
H
D002849:Chromatography, Gas; D032084:Citrus sinensis / Q000737:chemistry*; D009947:Organotin Compounds / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*
D009947:Organotin Compounds; D010573:Pesticide Residues; C022071:vendex