title: Methionine and selenium yeast supplementation of the maternal diets affects antioxidant activity of breeding eggs.
abstract: Four hundred fifty 52-wk-old Langshan layer hens (dual-purpose type, an indigenous poultry breed of China) were randomly divided into 9 treatments with 5 replicates in each treatment. Birds were fed corn-soybean diets (0.13 mg of Se/kg) supplemented with 0, 0.30, and 0.60 mg/kg of Se from Se yeast and 3.2, 4.0, and 5.4 g of dl-Met/kg, respectively. Increasing Se yeast supplementation significantly increased Se concentration in the egg yolk (P < 0.01) and the Se concentration of the 3.2 g of Met/kg treatment was higher than those of the 4.0 and 5.4 g of Met/kg treatments. Adding 0.3 mg of Se/kg to the diet significantly increased glutathione peroxidase (GSH-Px) activity in the egg yolk compared with 0 and 0.6 mg of Se/kg (P < 0.01) and increasing Se yeast supplementation significantly increased the GSH-Px activity in the egg albumen (P < 0.01). Increasing Met supplementation significantly decreased the GSH-Px activity in both the yolk and the albumen of the eggs (P < 0.01). Methionine supplemented at 3.2 and 4.0 g/kg significantly increased glutathione concentration in the egg yolk compared with 5.4 g of Met/kg (P < 0.01) and increasing Met supplementation increased the glutathione concentration in the egg albumen. Increasing Met supplementation significantly decreased malondialdehyde concentration in the egg yolk (P < 0.01) and Se supplemented at 0 and 0.6 mg/kg increased the malondialdehyde concentrations in the egg yolk compared with 0.3 mg of Se/kg (P < 0.01). Methionine supplemented at 4.0 and 5.4 g/kg significantly decreased carbonyl concentration compared with 3.2 g of Met/kg. The conclusion was drawn that Se yeast and Met supplementation of the maternal diets could enhance antioxidant activity of breeding eggs.
authors.affiliation: National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, P. R. China.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Wang
authors.forename: X J
authors.initials: XJ
authors.lastname: Pan
authors.forename: W Q
authors.initials: WQ
authors.lastname: Zhang
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Peng
authors.forename: R Q
authors.initials: RQ
authors.lastname: Zhao
authors.forename: G H
authors.initials: GH
authors.lastname: Zhou
mesh_terms: D000821:Animal Feed; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D019587:Dietary Supplements / Q000382:microbiology; D004530:Egg Yolk / Q000737:chemistry; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D008715:Methionine / Q000378:metabolism / Q000494:pharmacology*; D050050:Protein Carbonylation; D012643:Selenium / Q000378:metabolism / Q000494:pharmacology*; D015003:Yeasts
chemical_list: D008715:Methionine; D005979:Glutathione Peroxidase; D005978:Glutathione; D012643:Selenium