Selecting reference genes in the white blood cells of buffalos treated with recombinant growth hormone.
In this study, we assessed the expression stability of eight genes (GADPH, ACTB, 18S, YWHAZ, SDHA, HMBS, SF3A1, and EEF1A) in the white blood cells of lactating buffalos and their possible use as reference genes for studies on growth hormone (GH)-treated animals. All of the genes showed acceptable stability according to the threshold values suggested by some of the software that was used to analyze them, although the differences between the most stable (SF3A1 and ACTB) and the least stable (18S) were considerable. GH treatment did not influence their expression levels.
Castigliego
Lorenzo
L
Department of Animal Pathology, Prophylaxis and Food Hygiene, University of Pisa, Pisa 56124, Italy.
Armani
Andrea
A
Li
Xiaoning
X
Grifoni
Goffredo
G
Gianfaldoni
Daniela
D
Guidi
Alessandra
A
D000818:Animals; D002020:Buffaloes / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D005796:Genes; D013006:Growth Hormone / Q000008:administration & dosage / Q000378:metabolism*; D007774:Lactation; D007962:Leukocytes / Q000378:metabolism*
D013006:Growth Hormone