title: Kinetics and role of antibodies against intimin beta in colostrum and in serum from goat kids and longitudinal study of attaching and effacing Escherichia coli in goat kids.
abstract: The presence of antibodies to the intimin beta-binding region (Int280-beta) of attaching and effacing Escherichia coli (AEEC) in serum from 20 goat kids from 2 herds, as well as in goat colostrum, was investigated by enzyme-linked immunosorbent assay. In addition, the onset and subsequent pattern of shedding of AEEC from the same goat kids over a 6-mo period was investigated. All the colostrum and serum samples tested contained antibodies against Int280-beta. The association between the antibody titer and the isolation of AEEC suggests that antibodies to intimin beta do not prevent colonization of the intestine by AEEC in goat kids. The AEEC were generally shed only transiently. Most AEEC isolated from the kids belonged to serogroup O26. Three isolates belonged to serogroup O157. These data show that goat kids may be a reservoir of AEEC that are potentially pathogenic for humans.
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain. jaorden@vet.ucm.es
authors.forename: José A
authors.initials: JA
authors.lastname: Orden
authors.forename: Ricardo
authors.initials: R
authors.lastname: De la Fuente
authors.forename: María
authors.initials: M
authors.lastname: Yuste
authors.forename: Susana
authors.initials: S
authors.lastname: Martínez-Pulgarín
authors.forename: José A
authors.initials: JA
authors.lastname: Ruiz-Santa-Quiteria
authors.forename: Pilar
authors.initials: P
authors.lastname: Horcajo
authors.forename: Antonio
authors.initials: A
authors.lastname: Contreras
authors.forename: Antonio
authors.initials: A
authors.lastname: Sánchez
authors.forename: Juan C
authors.initials: JC
authors.lastname: Corrales
authors.forename: Gustavo
authors.initials: G
authors.lastname: Domínguez-Bernal
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000375:Aging; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D003126:Colostrum / Q000276:immunology*; D004197:Disease Reservoirs / Q000382:microbiology; D019453:Escherichia coli O157 / Q000276:immunology* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000276:immunology*; D006041:Goats / Q000276:immunology*; D007700:Kinetics; D008137:Longitudinal Studies
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli