Molecular characterization of beta-lactamase genes and their genetic structures in Acinetobacter genospecies 3 isolates in Taiwan.
The genetic structure of beta-lactamases in Acinetobacter genospecies 3 (AG3) isolates in Taiwan was studied to analyze their high rates of resistance to beta-lactams, including carbapenems (57.9%). bla(IMP-1) and bla(IMP-8) were located in a class 1 integron. bla(OXA-58) was bracketed by ISAba3. A novel TnpF-like integrase gene was identified upstream of bla(VEB-3). Adjacent to the 5' sequence of the bla(ADC) gene, folE was identified. Four new Acinetobacter-derived cephalosporinase (ADC) enzymes were found, which clustered phylogenetically with published AG3 ADC proteins.
Huang
Li-Yueh
LY
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
Lu
Po-Liang
PL
Chen
Te-Li
TL
Chang
Fang-Yee
FY
Fung
Chang-Phone
CP
Siu
L K
LK
D000150:Acinetobacter / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D002510:Cephalosporinase / Q000235:genetics; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D006801:Humans; D066298:In Vitro Techniques; D032023:Integrons / Q000235:genetics; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D013624:Taiwan / Q000453:epidemiology; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
D002510:Cephalosporinase; D001618:beta-Lactamases