title: The first report on the prevalence of pestivirus infection in camels in Sudan.
abstract: The role of pestivirus particularly bovine viral diarrhea virus (BVDV) in causing respiratory infections in camels was studied in four different localities in Sudan. The evaluation was carried out using ELISA, and positive specimens were further tested using direct fluorescent antibody technique (FAT) and reverse transcriptase polymerase chain reaction (RT-PCR) for confirmation. The overall detected seroprevalence of BVD in camel sera was 84.6% with the highest prevalence in Western Sudan (92.5%) and with most of positives showing 2+ and 3+ titer. Out of 186 lung specimens examined for BVDV antigen, 13 were found positive (7%) with the highest prevalence in Central Sudan. All ELISA-positive specimens were positive using FAT and RT-PCR. To our knowledge, this is the first report for the detection of BVDV antigen and antibodies in camels in Sudan.
authors.affiliation: Central Veterinary Research Laboratory, P.O. Box 8067, Al Amarat, Khartoum, Sudan. intisarkamil@yahoo.com
authors.forename: Kamil Saeed
authors.initials: KS
authors.lastname: Intisar
authors.forename: Yahia H
authors.initials: YH
authors.lastname: Ali
authors.forename: Abdelmelik I
authors.initials: AI
authors.lastname: Khalafalla
authors.forename: E A Rahman
authors.initials: EA
authors.lastname: Mahasin
authors.forename: Adil S
authors.initials: AS
authors.lastname: Amin
authors.forename: Khalid M
authors.initials: KM
authors.lastname: Taha
mesh_terms: D000818:Animals; D002162:Camelus / Q000821:virology*; D017931:DNA Primers / Q000235:genetics; D003710:Demography; D001908:Diarrhea Viruses, Bovine Viral; D004196:Disease Outbreaks / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D008168:Lung / Q000821:virology; D018182:Pestivirus Infections / Q000453:epidemiology* / Q000662:veterinary*; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000662:veterinary* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016036:Seroepidemiologic Studies; D013397:Sudan / Q000453:epidemiology
chemical_list: D017931:DNA Primers