title: Pouring linear gradient gels with a gradient former.
abstract: Linear gradient gels, which are polyacrylamide gels having a gradient of increasing acrylamide concentration (and linearly decreasing pore size), can have advantages over fixed-concentration SDS-PAGE gels. First, a much greater range of protein M(r) values can be separated on a linear gradient gel than on a fixed-concentration gel. Second, there is a greater likelihood of resolving proteins with very similar M(r) values on gradient gels than on fixed-concentration gels. This protocol describes the pouring of linear gradient gels with a gradient former. The most common gradient gel contains 4%-20% acrylamide; however, the range of acrylamide concentrations should be chosen on the basis of the size of the proteins being separated.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Simpson
mesh_terms: D000180:Acrylic Resins; D019411:Clinical Laboratory Techniques; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*
chemical_list: D000180:Acrylic Resins; C016680:polyacrylamide gels