Probing biomolecular interactions using surface enhanced Raman spectroscopy: label-free protein detection using a G-quadruplex DNA aptamer.
We demonstrate a strategy for label-free protein detection through monitoring the Surface Enhanced Raman Spectrum of an aptamer probe attached to a gold nanoshell. Low limit of detection and minimal non-specific binding show potential for in vitro and in vivo assays.
Ochsenkühn
Michael A
MA
School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh, EH9 3JJ, UK.
Campbell
Colin J
CJ
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D015374:Biosensing Techniques; D054856:G-Quadruplexes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013917:Thrombin / Q000032:analysis / Q000737:chemistry
D052157:Aptamers, Nucleotide; D011506:Proteins; D006046:Gold; D013917:Thrombin