title: Studying phenotypic evolution in domestic animals: a walk in the footsteps of Charles Darwin.
abstract: Charles Darwin used domesticated plants and animals as proof of principle for his theory on phenotypic evolution by means of natural selection. Inspired by Darwin's work, we developed an intercross between the wild boar and domestic pigs to study the genetic basis for phenotypic changes during domestication. The difference in coat color is controlled by two major loci. Dominant white color is due to two consecutive mutations in the KIT gene: a 450-kb duplication and a splice mutation. Black spotting is caused by the combined effect of two mutations in MC1R: a missense mutation for dominant black color and a 2-bp insertion leading to a frameshift. A major discovery made using this pedigree is the identification of a single-nucleotide substitution in intron 3 of the gene for insulin-like growth factor 2 (IGF2) that is underlying a quantitative trait locus affecting muscle growth, size of the heart, and fat deposition. The mutation disrupts the interaction with a repressor and leads to threefold increased IGF2 expression in postnatal muscle. In a recent study, we have identified the IGF2 repressor, and this previously unknown protein, named ZBED6, is specific for placental mammals and derived from a domesticated DNA transposon.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, SE-75124 Uppsala, Sweden. leif.andersson@imbim.uu.se
authors.forename: L
authors.initials: L
authors.lastname: Andersson
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000235:genetics* / Q000254:growth & development; D000835:Animals, Wild / Q000235:genetics / Q000254:growth & development; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D006824:Hybridization, Genetic; D007335:Insulin-Like Growth Factor II / Q000235:genetics; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D040641:Quantitative Trait Loci; D044102:Receptor, Melanocortin, Type 1 / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012880:Skin Pigmentation / Q000235:genetics; D034421:Sus scrofa / Q000235:genetics / Q000254:growth & development; D013552:Swine / Q000235:genetics / Q000254:growth & development
chemical_list: D004251:DNA Transposable Elements; D044102:Receptor, Melanocortin, Type 1; D007335:Insulin-Like Growth Factor II; D004247:DNA; D019009:Proto-Oncogene Proteins c-kit