title: Antibacterial effect of the bioactive compound beauvericin produced by Fusarium proliferatum on solid medium of wheat.
abstract: To obtain the bioactive compound beauvericin (BEA), Fusarium proliferatum CECT 20569 was grown on a solid medium of wheat, utilizing the technique of the solid state fermentation (SSF), being this mycotoxin purified by high performance liquid chromatography (HPLC) with a reverse phase semi-preparative column using as the mobile phase acetonitrile/water in gradient condition. The purity of the BEA was verified by analytical HPLC and liquid chromatography tandem mass spectrometry (LC/MS-MS). The pure fractions of BEA were utilized to determinate the antibiotic effects on several bacterial strains that are considered normally pathogens of the intestinal tract as: Escherichia coli, Enterococcus faecium, Salmonella enterica, Shigella dysenteriae, Listeria monocytogenes, Yersinia enterocolitica, Clostridium perfringens, Pseudomonas aeruginosa and Staphylococcus aureus.
authors.affiliation: Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain. giuseppe.meca@uv.es
authors.forename: G
authors.initials: G
authors.lastname: Meca
authors.forename: I
authors.initials: I
authors.lastname: Sospedra
authors.forename: J M
authors.initials: JM
authors.lastname: Soriano
authors.forename: A
authors.initials: A
authors.lastname: Ritieni
authors.forename: A
authors.initials: A
authors.lastname: Moretti
authors.forename: J
authors.initials: J
authors.lastname: Mañes
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002853:Chromatography, Liquid; D047630:Depsipeptides / Q000494:pharmacology*; D005670:Fusarium / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000378:metabolism* / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D047630:Depsipeptides; C004456:beauvericin