Characterization of susceptibility and carrier status of burbot, Lota lota (L.), to IHNV, IPNV, Flavobacterium psychrophilum, Aeromonas salmonicida and Renibacterium salmoninarum.
In this study, susceptibility and potential carrier status of burbot, Lota lota, were assessed for five important fish pathogens. Burbot demonstrated susceptibility and elevated mortality following challenge with infectious haematopoietic necrosis virus (IHNV) by immersion and to Aeromonas salmonicida by intraperitoneal (i.p.) injection. IHNV persisted in fish for at least 28 days, whereas A. salmonicida was not re-isolated beyond 17 days post-challenge. In contrast, burbot appeared refractory to Flavobacterium psychrophilum following intramuscular (i.m.) injection and to infectious pancreatic necrosis virus (IPNV) by immersion. However, i.p injection of IPNV resulted in re-isolation of virus from fish for the duration of the 28 day challenge. Renibacterium salmoninarum appeared to induce an asymptomatic carrier state in burbot following i.p. injection, but overt manifestation of disease was not apparent. Viable bacteria persisted in fish for at least 41 days, and bacterial DNA isolated by diagnostic polymerase chain reaction was detected from burbot kidney tissue 90 days after initial exposure. This study is the first to investigate susceptibility of burbot to selected fish pathogens, and this information will aid in efforts to culture and manage this species.
Polinski
M P
MP
Department of Fish and Wildlife Resources, College of Natural Resources, University of Idaho, Moscow, ID 83844-1136, USA.
Fehringer
T R
TR
Johnson
K A
KA
Snekvik
K R
KR
Lapatra
S E
SE
Lafrentz
B R
BR
Ireland
S C
SC
Cain
K D
KD
D048409:Aeromonas salmonicida / Q000502:physiology; D000818:Animals; D001424:Bacterial Infections / Q000382:microbiology / Q000401:mortality / Q000662:veterinary*; D018407:Bacterial Physiological Phenomena; D002353:Carrier State / Q000662:veterinary*; D004198:Disease Susceptibility / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D005417:Flavobacterium / Q000502:physiology; D048388:Gadiformes / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D029142:Infectious hematopoietic necrosis virus / Q000502:physiology; D018061:Infectious pancreatic necrosis virus / Q000502:physiology; D008835:Micrococcaceae / Q000502:physiology; D012327:RNA Virus Infections / Q000401:mortality / Q000662:veterinary* / Q000821:virology