Carbohydrate metabolism in mutants of the cyanobacterium Synechococcus elongatus PCC 7942 defective in glycogen synthesis.
ADP-glucose pyrophosphorylase (AGPase) and glycogen synthase (GS) catalyze the first two reactions of glycogen synthesis in cyanobacteria. Mutants defective in each of these enzymes in Synechococcus elongatus PCC 7942 were constructed and characterized. Activities of the corresponding enzymes in the selected mutants were virtually undetectable, and their ability to synthesize glycogen was entirely abolished. The maximal activities of photosynthetic O(2) evolution and the rates of respiration in the dark were significantly decreased in the mutants compared to those in wild-type cells. Addition of 0.2 M NaCl or 3 mM H(2)O(2) to liquid cultures markedly inhibited the growth of the AGPase and GS mutants, while the same treatment had only marginal effects on the wild type. These results suggest a significant role for storage polysaccharides in tolerance to salt or oxidative stress.
Suzuki
Eiji
E
Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University, Akita 010-0195, Japan. esuzuki@akita-pu.ac.jp
Ohkawa
Hajime
H
Moriya
Katsuya
K
Matsubara
Tatsuya
T
Nagaike
Yukari
Y
Iwasaki
Ikuko
I
Fujiwara
Shoko
S
Tsuzuki
Mikio
M
Nakamura
Yasunori
Y
D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D051599:Glucose-1-Phosphate Adenylyltransferase / Q000235:genetics / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis*; D006006:Glycogen Synthase / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009154:Mutation / Q000235:genetics*; D018384:Oxidative Stress / Q000187:drug effects; D010100:Oxygen / Q000032:analysis; D010788:Photosynthesis / Q000187:drug effects; D010798:Phycocyanin / Q000032:analysis; D012965:Sodium Chloride / Q000494:pharmacology; D046940:Synechococcus / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development
D002241:Carbohydrates; D010798:Phycocyanin; D002734:Chlorophyll; D012965:Sodium Chloride; D006003:Glycogen; D006861:Hydrogen Peroxide; D006006:Glycogen Synthase; D051599:Glucose-1-Phosphate Adenylyltransferase; D010100:Oxygen; D000077194:Chlorophyll A