title: [Establishment of a method for rapid detection of the nucleic acid of the novel A (H1N1) influenza virus].
abstract: A new flu caused by a novel influenza A(H1N1) virus has spread over the United States, Mexico and more than 40 other countries. And because of the immediate global concern, WHO has announced that the current level of influenza pandemic alert is raised to phase 5, indicating approaching of an influenza pandemic. As patients suffering from the influenza A (H1N1) have the similar symptoms as patients with seasonal influenza, differential detection and identification of the influenza virus have to depend on specific laboratory tests. We have successfully developed a RT-PCR based method for detection of the influenza A (H1N1) virus, and had applied the method to detection of clinical samples.
authors.affiliation: China CDC, Institute for Viral Disease Control and Prevention, Key laboratory for Virus Gene Engineer, Beijing 100052, China. dayanwang@yahoo.com.cn
authors.forename: Da-Yan
authors.initials: DY
authors.lastname: Wang
authors.forename: Rong-Bao
authors.initials: RB
authors.lastname: Gao
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Le-Ying
authors.initials: LY
authors.lastname: Wen
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: Zou
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
authors.forename: Xi-Yan
authors.initials: XY
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Lan
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Guo
authors.forename: Zi
authors.initials: Z
authors.lastname: Li
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Cheng
authors.forename: Min-Jju
authors.initials: MJ
authors.lastname: Tan
authors.forename: Xin-Wan
authors.initials: XW
authors.lastname: Li
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Zeng
authors.forename: Yuan-Ji
authors.initials: YJ
authors.lastname: Guo
authors.forename: De-Xin
authors.initials: DX
authors.lastname: Li
authors.forename: Yue-Long
authors.initials: YL
authors.lastname: Shu
mesh_terms: D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D012367:RNA, Viral