title: The NH(2)-terminal region of Streptococcus pyogenes M5 protein confers protection against degradation by proteases and enhances mucosal colonization of mice.
abstract: BACKGROUND
  The NH(2)-terminal sequence of the M protein from group A streptococci defines the serotype of the organism and contains epitopes that evoke bactericidal antibodies.
  
  
  METHODS
  To identify additional roles for this region of the M protein, we constructed a mutant of M5 group A streptococci expressing an M protein with a deletion of amino acid residues 3-22 (DeltaNH(2)).
  
  
  RESULTS
  M5 streptococci and the DeltaNH(2) mutant were resistant to phagocytosis and were similarly virulent in mice. However, DeltaNH(2) was significantly less hydrophobic, contained less lipoteichoic acid on its surface, and demonstrated reduced adherence to epithelial cells. These differences were abolished when organisms were grown in the presence of protease inhibitors. Treatment with cysteine proteases or with human saliva resulted in the release of M protein from the DeltaNH(2) mutant at a significantly greater rate than observed with the wild-type M5 strain. Compared with the parent strain, the DeltaNH(2) strain also showed a significant reduction in its ability to colonize the upper respiratory mucosa of mice.
  
  
  CONCLUSIONS
  The NH(2) terminus of M5 protein has an important role in protecting the surface protein from proteolytic cleavage, thus preserving its function as an anchor for lipoteichoic acid, which is a primary mediator of adherence to epithelial cells and colonization.
authors.affiliation: Department of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Penfound
authors.forename: Itzhak
authors.initials: I
authors.lastname: Ofek
authors.forename: Harry S
authors.initials: HS
authors.lastname: Courtney
authors.forename: David L
authors.initials: DL
authors.lastname: Hasty
authors.forename: James B
authors.initials: JB
authors.lastname: Dale
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D005260:Female; D051379:Mice; D020545:Respiratory Mucosa / Q000382:microbiology*; D013297:Streptococcus pyogenes / Q000276:immunology* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins