title: Microarray analysis of the gene expression profile in the midgut of silkworm infected with cytoplasmic polyhedrosis virus.
abstract: In order to obtain an overall view on silkworm response to Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) infection, a microarray system comprising 22,987 oligonucluotide 70-mer probes was employed to compare differentially expressed genes in the midguts of BmCPV-infected and normal silkworm larvae. At 72 h post-inoculation, 258 genes exhibited at least 2.0-fold differences in expression level. Out of these, 135 genes were up-regulated, while 123 genes were down-regulated. According to gene ontology (GO), 140 genes were classified into GO categories. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicates that 35 genes were involved in 10 significant (P<0.05) KEGG pathways. The expressions of genes related to valine, leucine, and isoleucine degradation, retinol metabolism, and vitamin B6 metabolism were all down-regulated. The expressions of genes involved in ribosome and proteasome pathway were all up-regulated. Quantitative real-time polymerase chain reaction was performed to validate the expression patterns of 13 selected genes of interest. The results suggest that BmCPV infection resulted in the disturbance of protein and amino acid metabolism and a series of major physiological and pathological changes in silkworm. Our results provide new insights into the molecular mechanism of BmCPV infection and host cell response.
authors.affiliation: School of Biotechnology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212018, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Xiu
authors.initials: X
authors.lastname: Wang
authors.forename: Guang-xing
authors.initials: GX
authors.lastname: Qin
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Jiang
authors.forename: Mu-Wang
authors.initials: MW
authors.lastname: Li
authors.forename: Xi-Jie
authors.initials: XJ
authors.lastname: Guo
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000821:virology*; D004064:Digestive System / Q000378:metabolism / Q000821:virology*; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D053263:Gene Regulatory Networks / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012087:Reoviridae / Q000502:physiology*; D012088:Reoviridae Infections / Q000235:genetics* / Q000821:virology; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000235:genetics