title: Uptake of functionalized mesoporous silica nanoparticles by human cancer cells.
abstract: Mesoporous silica nanoparticles (MSN) were functionalised by aminofluorescein (AMF) with diethylenetriaminepentaacetic acid spacer molecules which provide free carboxylic groups for binding cell-specific ligands such as folate. AMF allowed the exploration of cellular uptake by HeLa cells using confocal microscopy and flow cytometry. The functionalized nanoparticles (MSN-AMF) penetrated efficiently into HeLa cell cytoplasm through a clathrin dependent endocytosis mechanism. The number of endocytosed MSN-AMF was enhanced when using folate as a targeting molecule. Uptake kinetics revealed that most of MSN-AMF were internalized within 4 h of incubation. Moreover, we found that MSN-AMF were capable of escaping the acidic endolysosomal vesicles of HeLa cells. Cytotoxicity studies suggested that these nanoparticles are non-toxic to HeLa cells up to a dose level of 50 microg/ml.
authors.affiliation: Centre de Recherche sur la Matière Divisée-UMR6619 Université d'Orléans-CNRS, 1B rue de la Férollerie, 45071 Orleáns Cedex 2, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Fisichella
authors.forename: Hinda
authors.initials: H
authors.lastname: Dabboue
authors.forename: Sanjib
authors.initials: S
authors.lastname: Bhattacharyya
authors.forename: Gérald
authors.initials: G
authors.lastname: Lelong
authors.forename: Marie-Louise
authors.initials: ML
authors.lastname: Saboungi
authors.forename: Fabienne
authors.initials: F
authors.lastname: Warmont
authors.forename: Patrick
authors.initials: P
authors.lastname: Midoux
authors.forename: Chantal
authors.initials: C
authors.lastname: Pichon
authors.forename: Martine
authors.initials: M
authors.lastname: Guérin
authors.forename: Tobias
authors.initials: T
authors.lastname: Hevor
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Salvetat
mesh_terms: D003460:Crystallization / Q000379:methods*; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D046911:Macromolecular Substances; D012822:Silicon Dioxide