Simultaneous HPLC-F analysis of three recent antiepileptic drugs in human plasma.
An original high-performance liquid chromatographic method with fluorescence detection is presented for the simultaneous determination of the three antiepileptic drugs gabapentin, vigabatrin and topiramate in human plasma. After pre-column derivatisation with dansyl chloride, the analytes were separated on a Hydro-RP column with a mobile phase composed of phosphate buffer (55%) and acetonitrile (45%) and detected at lambda(em)=500 nm, exciting at 300 nm. An original pre-treatment procedure on biological samples, based on solid-phase extraction with MCX cartridges for gabapentin and vigabatrin, and with Plexa cartridges for topiramate, gave high extraction yields (>91%), satisfactory precision (RSD<6.4%) and good selectivity. Linearity was found in the 0.2-50.0 microg mL(-1) range for gabapentin, in the 1.0-100.0 microg mL(-1) range for vigabatrin and in the 1.0-50.0 microg mL(-1) range for topiramate, with limits of detection (LODs) between 0.1 and 0.3 microg mL(-1). After validation, the method was successfully applied to some plasma samples from patients undergoing therapy with one or more of these drugs. Accuracy results were satisfactory (recovery >91%). Therefore, the method seems to be suitable for the therapeutic drug monitoring (TDM) of patients treated with gabapentin, vigabatrin and topiramate.
Mercolini
Laura
L
Faculty of Pharmacy, Department of Pharmaceutical Sciences, Alma Mater Studiorum-University of Bologna, Via Belmeloro 6, I-40126 Bologna, Italy.
Mandrioli
Roberto
R
Amore
Mario
M
Raggi
Maria Augusta
MA
D000588:Amines / Q000097:blood* / Q000737:chemistry; D000927:Anticonvulsants / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003509:Cyclohexanecarboxylic Acids / Q000097:blood* / Q000737:chemistry; D003619:Dansyl Compounds / Q000737:chemistry; D016903:Drug Monitoring / Q000379:methods; D005632:Fructose / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D000077206:Gabapentin; D006801:Humans; D057230:Limit of Detection; D013050:Spectrometry, Fluorescence / Q000379:methods*; D000077236:Topiramate; D020888:Vigabatrin / Q000097:blood* / Q000737:chemistry; D005680:gamma-Aminobutyric Acid / Q000097:blood* / Q000737:chemistry
D000588:Amines; D000927:Anticonvulsants; D003509:Cyclohexanecarboxylic Acids; D003619:Dansyl Compounds; D000077236:Topiramate; D005632:Fructose; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin; D020888:Vigabatrin; C029038:dansyl chloride