Two closely related subclass II SnRK2 protein kinases cooperatively regulate drought-inducible gene expression.
The subclass III group of SNF1-related protein kinase 2 (SnRK2) members is known to play an important role in ABA and osmotic stress signaling in Arabidopsis; however, the roles of other subclasses remain elusive. Here, we established a double mutant of SRK2C/SnR2.8 and SRK2F/SnRK2.7 to investigate the functions of subclass II SnRK2s. Microarray analysis suggested that subclass II SnRK2s regulate some drought-responsive genes involving ABA-responsive element binding transcription factors (AREB/ABF) and their targets, and quantitative reverse transcription- PCR confirmed that those genes were down-regulated significantly in srk2cf. This study indicates that subclass II SnRK2s also play important roles in drought stress signaling in Arabidopsis.
Mizoguchi
Masahide
M
RIKEN Plant Science Center, 3-1-1, Kouyadai, Tsukuba, Ibaraki, 305-0074 Japan.
Umezawa
Taishi
T
Nakashima
Kazuo
K
Kidokoro
Satoshi
S
Takasaki
Hironori
H
Fujita
Yasunari
Y
Yamaguchi-Shinozaki
Kazuko
K
Shinozaki
Kazuo
K
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological
D029681:Arabidopsis Proteins; D018749:RNA, Plant; C492978:SnRK2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases