[Effect of Arg-Gly-Asp peptide on the secretion of vascular endothelial growth factor from vascular endothelial cells].
OBJECTIVE
To investigate the effect of Arg-Gly-Asp peptides (RGD peptides)on the secretion of vascular endothelial growth factor (VEGF) from vascular endothelial cells (VEC) and the mechanism.


METHODS
Using VEC cultured from the arteries of human greater omentum as a model, and detecting the content of VEGF and the activity of protein kinase C (PKC) under different concentration of RGD.


RESULTS
After incubation with 100-300 mg/ml of RGD peptide for 24h, the VEGF content and the activity of PKC in experimental group was significantly higher than that in control group (P < 0.01).


CONCLUSIONS
The stimulation of RGD peptide to the secretion of VEGF from VEC is in a dose-dependent way, and the stimulation is regulated by activating PKC.
Guan
Qing
Q
School of Nursing, Jilin Medical College, Jilin 132013, China. georgina27@sina.com
Li
Zhongyan
Z
Uumar
Soban
S
Lü
Shijie
S
D001158:Arteries / Q000166:cytology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D009842:Oligopeptides / Q000494:pharmacology*; D009852:Omentum / Q000098:blood supply; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D009842:Oligopeptides; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C047981:arginyl-glycyl-aspartic acid