Structure of Agaricus spp. fucogalactans and their anti-inflammatory and antinociceptive properties.
Fucogalactans from Agaricus brasiliensis (EPF-Ab) and A. bisporus var. hortensis (EPF-Ah) were prepared via by aqueous extraction and a purification procedure. EPF-Ab had M(w) 19.4 x 10(3)g/mol and EPF-Ah M(w) 31.1 x 10(3)g/mol. EPF-Ab had a (1-->6)-linked alpha-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of alpha-L-Fucp. EPF-Ah had a similar main-chain with O-2 substitution, but was partially methylated at HO-3, as well as having 2.5% non-reducing end-units of beta-D-Gal. In mice, EPF-Ab gave 39% antinociceptive inhibition (ID(50)>100mg/kg) and no anti-inflammatory activity. EPF-Ah also gave an inhibition of 39% at ID(50) 0.33 mg/kg and also inhibited by 61% (ID(50) 5.0mg/kg) total cell migration and by 32% peritoneal capillary permeability, which is related to the anti-inflammatory effect. The small differences in chemical structure in these polysaccharides thus modified their biological activities.
Komura
Dirce L
DL
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba 81531-980, Brazil.
Carbonero
Elaine R
ER
Gracher
Ana Helena P
AH
Baggio
Cristiane H
CH
Freitas
Cristina S
CS
Marcon
Rodrigo
R
Santos
Adair R S
AR
Gorin
Philip A J
PA
Iacomini
Marcello
M
D000364:Agaricus / Q000378:metabolism*; D000700:Analgesics / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D005638:Fruit / Q000378:metabolism; D005685:Galactans / Q000008:administration & dosage* / Q000737:chemistry; D007249:Inflammation / Q000175:diagnosis / Q000188:drug therapy*; D008297:Male; D051379:Mice; D010146:Pain / Q000175:diagnosis / Q000188:drug therapy*; D010936:Plant Extracts / Q000494:pharmacology*; D016896:Treatment Outcome
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D005685:Galactans; D010936:Plant Extracts; C031159:fucogalactan