Application of peptide nucleic acid towards development of nanobiosensor arrays.
Peptide nucleic acid (PNA) is the modified DNA or DNA analogue with a neutral peptide backbone instead of a negatively charged sugar phosphate. PNA exhibits chemical stability, resistant to enzymatic degradation inside living cell, recognizing specific sequences of nucleic acid, formation of stable hybrid complexes like PNA/DNA/PNA triplex, strand invasion, extraordinary thermal stability and ionic strength, and unique hybridization relative to nucleic acids. These unique physicobiochemical properties of PNA enable a new mode of detection, which is a faster and more reliable analytical process and finds applications in the molecular diagnostics and pharmaceutical fields. Besides, a variety of unique characteristic features, PNAs replace DNA as a probe for biomolecular tool in the molecular genetic diagnostics, cytogenetics, and various pharmaceutical potentials as well as for the development of sensors/arrays/chips and many more investigation purposes. This review paper discusses the various current aspects related with PNAs, making a new hot device in the commercial applications like nanobiosensor arrays.
Singh
Ravindra P
RP
Interdisciplinary Program of Integrated Biotechnology, Sogang University, 1 Sinsoo-Dong, Mapo-Gu, Seoul 121-742, Republic of Korea. rpsnpl69@gmail.com
Oh
Byung-Keun
BK
Choi
Jeong-Woo
JW
D015374:Biosensing Techniques / Q000379:methods*; D003582:Cytogenetics; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D046228:Microarray Analysis / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D020135:Peptide Nucleic Acids; D004247:DNA