Opposite effects of Trichostatin A on activation of mast cells by different stimulants.
Mast cells (MCs) are activated upon stimulation via TLRs or FcepsilonRI, contributing to immune protection and/or leading to allergic diseases. In the present study, the effects of Trichostatin A (TSA) on the activation of MCs were analyzed with bone marrow-derived (BM) MCs. TSA increased the transcription and protein secretion of IL-6 in case of LPS-stimulation, in contrast to the suppressive effect on IgE-mediated activation of BMMCs. Chromatin immunoprecipitation assay showed IgE-mediated signaling-specific suppression of transcription factors recruitment to the IL-6 promoter. TSA-treatment inhibited nuclear translocation of NF-kappaB following IgE-mediated, but not LPS-induced activation in MCs.
Wang
Qing-hui
QH
Atopy Research Center, Juntendo University School of Medicine, Tokyo, Japan.
Nishiyama
Chiharu
C
Nakano
Nobuhiro
N
Kanada
Shunsuke
S
Hara
Mutsuko
M
Kitamura
Nao
N
Shimokawa
Naomi
N
Lu
Chang-long
CL
Ogawa
Hideoki
H
Okumura
Ko
K
D006877:Hydroxamic Acids / Q000494:pharmacology*; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D006877:Hydroxamic Acids; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014157:Transcription Factors; C012589:trichostatin A