title: [Identification of asthenozoospermia-associated proteins in human seminal plasma by shotgun proteomic strategy].
abstract: OBJECTIVE
  To identify asthenozoospermia-associated proteins in seminal plasma by the shotgun proteomic strategy.
  
  
  METHODS
  Six seminal plasma samples were collected by Percoll respectively from healthy fertile and asthenozoospermia volunteers, balanced, mixed, and then the mixture was separated by SDS-PAGE. The proteins in the gel were enzymolyzed, extracted and identified by the shotgun proteomic strategy. The identified proteins with the unique peptide count > or =2 or the unique peptide count=1 but the total count > or =4 were compared between the two groups.
  
  
  RESULTS
  A total of 172 differential proteins were identified, of which, 89 were exclusively from the asthenozoospermia and 83 exclusively from the healthy fertile men. According to the molecular function, these differential proteins were mainly the types of signal transduction and catalytic activity.
  
  
  CONCLUSIONS
  Functionally, 10 of the proteins are particularly important, which include annexin VI isoform 2, isoform 1 of interleukin-6 receptor subunit beta precursor, Mr 400,000 protein, cytosolic dynein heavy chain, alpha-actinin-4, receptor-type tyrosine-protein phosphatase eta precursor, vitamin D-binding protein precursor, protein S100-A11, protein S100-A9 and ANXA4.
authors.affiliation: Clinical Laboratory, PLA General Hospital, Beijing 100853, China. jiebai69@tom.com
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
authors.forename: Shu-hong
authors.initials: SH
authors.lastname: Fu
authors.forename: Li-li
authors.initials: LL
authors.lastname: Cai
authors.forename: Ling
authors.initials: L
authors.lastname: Sun
authors.forename: Yu-long
authors.initials: YL
authors.lastname: Cong
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000503:physiopathology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D040901:Proteomics; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000302:isolation & purification*; D014809:Vitamin D-Binding Protein / Q000302:isolation & purification
chemical_list: D029589:Seminal Plasma Proteins; D014809:Vitamin D-Binding Protein