Characterization of a rolling-circle replication plasmid pXY3 from Lactobacillus plantarum XY3.
The complete nucleotide sequence of cryptic plasmid pXY3 isolated from Lactobacillus plantarum strain XY3 has been determined. It consisted of a 2968-bp circular molecule with a G+C content of 39%. Sequence analysis of pXY3 revealed three putative open reading frames (ORFs). Based on sequence similarity, the Rep protein shared 89% and 88% identity with Rep proteins of pLF24 and pWCFS102, respectively, which belonged to the rolling-circle replication (RCR) pMV158 family. A ssoT-like single-strand origin (sso) and a typical pMV158 family double-strand origin (dso) located upstream of the rep gene. Southern blot analysis indicated pXY3 replicate via a rolling-circle (RC) mechanism. Furthermore, the relative copy number of pXY3 was estimated to be about 97 copies per chromosome equivalent by real-time PCR.
Zhou
Hui
H
Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, College of Food Science & Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, China.
Hao
Yanling
Y
Xie
Ying
Y
Yin
Sheng
S
Zhai
Zhengyuan
Z
Han
Beizhong
B
D001483:Base Sequence; D004261:DNA Replication / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004270:DNA, Circular / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D018628:Gene Dosage / Q000235:genetics; D048191:Lactiplantibacillus plantarum / Q000235:genetics*; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification; D018741:Replication Origin / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004270:DNA, Circular; D004277:DNA, Single-Stranded