title: Production of bioemulsifier by Acinetobacter species isolated from healthy human skin.
abstract: Six Acinetobacter sp. isolated from healthy human skin were checked for the production of bioemulsifier. Optimization studies indicated that Luria Bertani broth pH 7 supplemented with calcium chloride (1%) was the optimum medium. Temperature at 37 degrees C was optimum and inducer oils in the medium did not enhance bioemulsifier production. Partial purification of bioemulsifier and chemical analysis revealed that it is a proteoglycan with protein (53%), polysaccharide (43%) and lipid (2%). Maximum emulsification activity obtained was 400 EU/ml. Thin layer chromatography revealed the presence of mannose and rhamnose sugar and oleic and palmitic acids as parts of lipids. The yield obtained was 1.9 g / 1. Reconstitution studies revealed that the protein and polysaccharide fractions together display 94.55% of emulsification activity. It was also noted that the bioemulsifier was stable for 72 hr at 37 degrees C and displayed good cleaning property towards different oils. The partially purified bioemulsifier formed stable oil-in-water emulsions with plant oils.
authors.affiliation: Department of Microbiology, University of Pune, Pune 411 007, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Jagtap
authors.forename: Supriya
authors.initials: S
authors.lastname: Yavankar
authors.forename: Karishma
authors.initials: K
authors.lastname: Pardesi
authors.forename: Balu
authors.initials: B
authors.lastname: Chopade
mesh_terms: D000150:Acinetobacter / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism*; D045703:Emulsifying Agents / Q000302:isolation & purification / Q000378:metabolism*; D004655:Emulsions; D006801:Humans; D010938:Plant Oils / Q000494:pharmacology; D012867:Skin / Q000382:microbiology*
chemical_list: D045703:Emulsifying Agents; D004655:Emulsions; D010938:Plant Oils