title: Nondestructive imaging of individual biomolecules.
abstract: Radiation damage is considered to be the major problem that still prevents imaging an individual biological molecule for structural analysis. So far, all known mapping techniques using sufficient short wavelength radiation, be it x rays or high energy electrons, circumvent this problem by averaging over many molecules. Averaging, however, leaves conformational details uncovered. Even the anticipated use of ultrashort but extremely bright x-ray bursts of a free electron laser shall afford averaging over 10{6} molecules to arrive at atomic resolution. Here, we present direct experimental evidence for nondestructive imaging of individual DNA molecules. In fact, we show that DNA withstands coherent low energy electron radiation with deBroglie wavelength in the Angstrom regime despite a vast dose of 10{8} electrons/nm{2} accumulated over more than one hour.
authors.affiliation: Institute of Physics, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Germann
authors.forename: Tatiana
authors.initials: T
authors.lastname: Latychevskaia
authors.forename: Conrad
authors.initials: C
authors.lastname: Escher
authors.forename: Hans-Werner
authors.initials: HW
authors.lastname: Fink