title: Membrane proteome of the green sulfur bacterium Chlorobium tepidum (syn. Chlorobaculum tepidum) analyzed by gel-based and gel-free methods.
abstract: Chlorobium tepidum is a Gram-negative bacterium of the green sulfur phylum (Chlorobia). Chlorobia are obligate anaerobic photolithoautotrophs that are widely distributed in aquatic environments where anoxic layers containing reduced sulfur compounds are exposed to light. The envelope of C. tepidum is a complex organelle composed of the outer membrane, the periplasm-peptidoglycan layer, and the cytoplasmic membrane. In addition to the outer and plasma membranes, C. tepidum contains chlorosomes attached to the cytoplasmic side of the plasma membrane. Each cellular compartment has a unique set of proteins, called sub-proteome. An important aim of proteome analysis is to study the level of the expressed genes and their response to environmental changes. Membrane protein studies are of primary importance to understand how nutrients are transported inside the cell, how toxic molecules are exported, and the mechanisms of photosynthesis and energy metabolism.
authors.affiliation: Division of Biochemistry, Department of Chemistry, University of Crete, Voutes, Heraklion, Greece.
authors.forename: Kalliopi
authors.initials: K
authors.lastname: Kouyianou
authors.forename: Michalis
authors.initials: M
authors.lastname: Aivaliotis
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
authors.forename: Michael
authors.initials: M
authors.lastname: Karas
authors.forename: Georgios
authors.initials: G
authors.lastname: Tsiotis
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000378:metabolism*; D041883:Chlorobium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008565:Membrane Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012995:Solubility / Q000187:drug effects
chemical_list: D001426:Bacterial Proteins; D003902:Detergents; D008565:Membrane Proteins; D020543:Proteome