title: Application of "homogeneous assay for fluorescence concentrated on membrane" to the analysis of the substrate specificity of protease.
abstract: The utility of the homogeneous assay for fluorescence concentrated on membrane (HAFCOM) in the analysis of the substrate specificity of protease was investigated using tobacco etch virus (TEV) protease. The V(max) of TEV protease against variants of a substrate was obtained by a simple procedure. It was considered that HAFCOM was more accurate than other endpoint measurements of protease assay.
authors.affiliation: Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Japan. tsuchida@hoku-iryo-u.ac.jp
authors.forename: Shirou
authors.initials: S
authors.lastname: Tsuchida
authors.forename: Takashi
authors.initials: T
authors.lastname: Shigematsu
authors.forename: Taemi
authors.initials: T
authors.lastname: Yahara
authors.forename: Kana
authors.initials: K
authors.lastname: Nunome
authors.forename: Naoya
authors.initials: N
authors.lastname: Hamaue
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005453:Fluorescence; D013379:Substrate Specificity / Q000235:genetics
chemical_list: D010450:Endopeptidases; C099488:TEV protease