Synchrotron FTIR micro-spectroscopy study of the rat hippocampal formation after pilocarpine-evoked seizures.
In the present work, synchrotron radiation Fourier transform infrared (SRFTIR) micro-spectroscopy and imaging were used for topographic and semi-quantitative biochemical analysis of rat brain tissue in cases of pilocarpine-induced epilepsy. The tissue samples were analyzed with a beam defined by small apertures and spatial resolution steps of 10 microm which allowed us to probe the selected cellular layers of hippocampal formation. Raster scanning of the samples has generated 2D chemical cartographies revealing the distribution of proteins, lipids and nucleic acids. Spectral analysis has shown changes in the saturation level of phospholipids and relative secondary structure of proteins. Special interest was put in the analysis of two areas of the hippocampal formation (sector 3 of the Ammon's horn, CA3 and dentate gyrus, DG) in which elemental abnormalities were observed during our previous studies. Statistically significant increase in the saturation level of phospholipids (increased ratio of the absorption intensities at around 2921 and 2958 cm(-1)) as well as conformational changes of proteins (beta-type structure discrepancies as shown by the increased ratio of the absorbance intensities at around 1631 and 1657 cm(-1) as well as the ratio of the absorbance at 1548 and 1657 cm(-1)) were detected in pyramidal cells of CA3 area as well as in the multiform and molecular layers of DG. The findings presented here suggest that abnormalities in the protein secondary structure and increases in the level of phospholipid saturation could be involved in mechanisms of neurodegenerative changes following the oxidative stress evoked in brain areas affected by pilocarpine-induced seizures.
Chwiej
J
J
Faculty of Physics and Applied Computer Science, AGH-University of Science and Technology, Krakow, Poland. jchwiej@novell.ftj.agh.edu.pl
Dulinska
J
J
Janeczko
K
K
Dumas
P
P
Eichert
D
D
Dudala
J
J
Setkowicz
Z
Z
D000818:Animals; D006624:Hippocampus / Q000378:metabolism*; D015227:Lipid Peroxidation; D010862:Pilocarpine; D017966:Pyramidal Cells / Q000378:metabolism*; D051381:Rats; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D018709:Statistics, Nonparametric
D010862:Pilocarpine