title: Expression and localization of Bombyx mori V-ATPase 16 kDa subunit c.
abstract: V-ATPase plays a central role in lepidopteran midgut ion transport physiology, and lepidopteran midgut turned out to be a model tissue for the study of V-ATPase. In the present study, the 5'-RACE method is used to obtain the 5'-UTR of V-ATPase c subunit gene from Bombyx mori. Sequence analysis of the promoter region and 3'-UTR of V-ATPase c subunit gene revealed that the transcription of the V-ATPase c subunit gene may be regulated by multi-ways. RT-PCR analysis showed that B. mori V-ATPase c subunit mRNA expresses in the whole developmental stages of B. mori. We also constructed a transient vector to determine the subcellular localization of the B. mori V-ATPase c subunit, and the result demonstrated that it is located in the membrane and some specific regions of BmN cells. Real-time PCR analysis further indicated that the c subunit mRNA expression was upregulated significantly at 24 and 72 h in the midguts of resistant B. mori larvae after being inoculated with B. mori nucleopolyhedrovirus, suggesting that it may be related to the immune response against virus infection.
authors.affiliation: Institute of Life Sciences, Jiangsu University, No. 301 Xuefu Road, Zhenjiang 212013, P. R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lü
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.forename: Lu
authors.initials: L
authors.lastname: Gao
authors.forename: Ye
authors.initials: Y
authors.lastname: Pan
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: He
authors.forename: Guoping
authors.initials: G
authors.lastname: Huang
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D012831:Bombyx / Q000201:enzymology*; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D005822:Genetic Vectors; D007814:Larva / Q000201:enzymology; D011401:Promoter Regions, Genetic; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D017931:DNA Primers; D021122:Protein Subunits; D012333:RNA, Messenger; D000251:Adenosine Triphosphatases