title: Implication of discoidin domain receptor 1 in T cell migration in three-dimensional collagen.
abstract: T cell migration through extracellular matrix of the tissue is an important process in the development of inflammation. However, the mechanisms regulating this process are complex and still not well defined. In this study, we show that activation of human peripheral blood T cells with anti-CD3 mAb increases the mRNA and protein levels of the discoidin domain receptor 1 (DDR1), which is known to bind to collagens. Furthermore, our findings indicate that DDR1 is involved in the migration of activated T cells in three-dimensional (3D) collagen. Indeed, the use of a DDR1 blocking molecule (DDR1:Fc) reduced the capacity of anti-CD3-activated human T cells to migrate in 3D collagen, whereas a control immunoglobulin had no effect. As a control, the DDR1:Fc molecule did not interfere with the capacity of human T cells to migrate through fibronectin. Together these results suggest that DDR1 can represent an additional receptor regulating T cell movement in the tissues and therefore can contribute to the development of inflammatory diseases.
authors.affiliation: Centre de Recherche en Rhumatologie/Immunologie, Centre Hospitalier Universitaire de Québec, Pavillon CHUL, and Faculté de Médecine, Université Laval 2705 Blvd Laurier, Local T1-49, Ste-Foy, Québec, PQ G1V 4G2 Canada.
authors.forename: Lamia Naouel
authors.initials: LN
authors.lastname: Hachehouche
authors.forename: Nizar
authors.initials: N
authors.lastname: Chetoui
authors.forename: Fawzi
authors.initials: F
authors.lastname: Aoudjit
mesh_terms: D000328:Adult; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology*; D000070818:Discoidin Domain Receptors; D015870:Gene Expression; D006801:Humans; D019169:Jurkat Cells; D016853:Muromonab-CD3 / Q000494:pharmacology; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011975:Receptors, Mitogen / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
chemical_list: D016853:Muromonab-CD3; D011975:Receptors, Mitogen; D003094:Collagen; D000070818:Discoidin Domain Receptors; D020794:Receptor Protein-Tyrosine Kinases