Protective effects of fraction 1a of polysaccharides isolated from Solanum nigrum Linne on thymus in tumor-bearing mice.
OBJECTIVE
To further screen out the anti-tumor active compound of polysaccharides isolated from Solanum nigrum Linne (SNL-P), which had shown to have anti-cervical cancer and modulating properties, and evaluate the thymus protective effects of this active compound.


METHODS
SNL-P was separated and purified by column chromatography, and its anti-cervical cancer activity was evaluated by mice models injected of ascites U14 cells. Furthermore, the protective effect of fraction 1a of SNL-P (SNL-P1a) on the thymus tissue of tumor-bearing mice was evaluated by histological study and TUNEL staining. Finally, the protein expression of Bcl-2 and Bax gene were assayed by immunohistochemistry.


RESULTS
SNL-P1a has shown a marked inhibition effect on U14 cevical cancer, it restore the ratio of CD4(+)/CD8(+) peripheral blood T-lymphocyte subpopulation. Histological study and TUNEL staining results showed that SNL-P1a protect thymus tissue against the onslaught of tumor by inhibiting thymus lymphocyte apoptosis, and immunohistochemistry assay displayed that SNL-P1a treatment could increase Bcl-2/Bax ratio in thymus lymphocytes of tumor-bearer, which might promote more thymus lymphocytes towards proliferation.


CONCLUSIONS
SNL-P1a had significant growth inhibition effect on U14 cervical cancer and protective effect on thymus tissue of tumor-bearing mice.
Li
Jian
J
College of Environmental & Chemical Engineering, Yanshan University, Qinhuangdao, Hebei Province, China.
Li
Qingwang
Q
Peng
Yong
Y
Zhao
Rui
R
Han
Zengsheng
Z
Gao
Dawei
D
D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D016516:CD4-CD8 Ratio; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D020287:In Situ Nick-End Labeling; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D032324:Solanum nigrum / Q000737:chemistry*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; D011134:Polysaccharides