title: Gene expression profile of HIRRV G and N protein gene vaccinated Japanese flounder, Paralichthys olivaceus during HIRRV infection.
abstract: The glycoprotein (G protein) gene, but not the nucleocapsid protein (N protein) gene, of the hirame rhabdovirus (HIRRV) was previously shown to be highly effective in inducing a protective immune response in Japanese flounder (Paralichthys olivaceus) when used as a DNA vaccine. Our previous cDNA microarray analysis demonstrated that interferon-stimulated genes (ISGs) were strongly induced by the HIRRV G protein gene (pHRV-G) but not by the N protein gene (pHRV-N). However, the molecular basis for the difference in protective immunity between pHRV-G- and pHRV-N-vaccinated fish during HIRRV infection is still unclear. In this study, we use a DNA microarray to analyze differences of gene expression in pHRV-G- and pHRV-N-vaccinated fish during HIRRV infection. Microarray analyses showed substantial difference in gene expression patterns during HIRRV infection between fish vaccinated with pHRV-G and pHRV-N. In addition, genes having homology to mammalian T cell activation-related genes were up-regulated in the HIRRV G protein-vaccinated group.
authors.affiliation: Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
authors.forename: Motoshige
authors.initials: M
authors.lastname: Yasuike
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000818:Animals; D002460:Cell Line; D016000:Cluster Analysis; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000276:immunology; D005432:Flounder / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling / Q000662:veterinary*; D005786:Gene Expression Regulation / Q000276:immunology; D029141:Novirhabdovirus / Q000235:genetics / Q000276:immunology; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D018353:Rhabdoviridae Infections / Q000276:immunology / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D029941:Fish Proteins; D019590:Nucleocapsid Proteins; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines