Disinfection kinetics of murine norovirus using chlorine and chlorine dioxide.
We determined the disinfection efficiency of chlorine and chlorine dioxide (ClO(2)) using murine norovirus (MNV) and coliphage MS2 as surrogates for human norovirus. Experiments were performed in oxidant demand-free buffer (pH 7.2) at 5 degrees C and 20 degrees C. The extent of virus inactivation by a disinfectant was quantified using three different analytical methods: plaque, short template real-time TaqMan reverse transcriptase-polymerase chain reaction (RT-PCR), and long template RT-PCR assays. Rapid inactivation of MNV by both chlorine and chlorine dioxide was observed by the plaque assay. According to the efficiency factor Hom model, Ct values of 0.314mg/Lmin and 0.247mg/Lmin were required for a 4-log reduction of MNV at 5 degrees C by chlorine and chlorine dioxide, respectively. Lower Ct values were required at 20 degrees C. Both long template and short template RT-PCR assays significantly underestimated the virus inactivation compared to the plaque assay. Our study demonstrates that adequate treatment of water with either chlorine or ClO(2) is likely to effectively control the waterborne transmission of human norovirus.
Lim
Mi Young
MY
Department of Environmental Health, and Institute of Health and Environment, School of Public Health, Seoul National University, 599 Kwanak-ro Kwanak-gu, Seoul 151-747, Republic of Korea.
Kim
Ju-Mi
JM
Ko
Gwangpyo
G
D000818:Animals; D002460:Cell Line; D002713:Chlorine / Q000494:pharmacology*; D017606:Chlorine Compounds / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017909:Levivirus / Q000187:drug effects / Q000235:genetics; D051379:Mice; D029322:Norovirus / Q000187:drug effects* / Q000235:genetics; D010087:Oxides / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017606:Chlorine Compounds; D010087:Oxides; D002713:Chlorine; C025109:chlorine dioxide