[Effects of mixed carbon sources in cultivation of recombinant Pichia pastoris for polygalacturonate lyase production].
In order to increase the production and productivity of alkaline polygalacturonate lyase (PGL), we studied the mixed carbon sources feeding strategies during the induction phase by recombinant Pichia pastoris GS 115. Glycerol, sorbitol or lactic acid co-feeding with methanol all enhanced the PGL production. Among all the feeding strategies, the sorbitol co-feeding strategy was most significant. By using this strategy, the PGL activity and productivity reached 1593 U/mL and 16.7 U/(mL-h). Compared to the control, the enhancements of PGL activity and productivity were 84.6% and 45.2% respectively, when we set the sorbitol feeding rate at 3.6 g/(h x L).
Wang
Zhihao
Z
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
Zhang
Dongxu
D
Li
Jianghua
J
Du
Guocheng
G
Chen
Jian
J
D002244:Carbon / Q000378:metabolism*; D046508:Culture Techniques; D000432:Methanol / Q000378:metabolism; D010843:Pichia / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D011133:Polysaccharide-Lyases / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D013012:Sorbitol / Q000378:metabolism
D011994:Recombinant Proteins; D013012:Sorbitol; D002244:Carbon; D011133:Polysaccharide-Lyases; C023911:pectate lyase; D000432:Methanol