[Changes of dendritic cells and its subsets in peripheral blood of patients with Mycobacterium tuberculosis].
OBJECTIVE
To analyze the changes of dendritic cells and its subsets in peripheral blood of patients with mycobacterium tuberculosis, and to explore its immune mechanism.


METHODS
32 patients with mycobacterium tuberculosis and 11 healthy controls were selected, who were diagnosed in our hospital from Nov. 2008 to Aug. 2009. The patients included 19 initial-treatment cases and 13 retreatment cases, and 19 lung tuberculosis patients with different sputum tests. Dendritic cells and its subsets in peripheral blood were detected by using flow cytometry.


RESULTS
DC1 subset and Total DCs in experimental group were (0.28+/-0.13)%, (0.42+/-0.19)% respectively, which were significant lower than they were in control group (0.47+/-0.23)%, (0.65+/-0.22)% respectively (P<0.01); DC1 subset and Total DCs in sputum positive group were (0.16+/-0.04)%, (0.24+/-0.06)% respectively, which were significant lower than they were in sputum negative group(0.28+/-0.14)%, (0.43+/-0.12)% (P<0.05); There was no significance in Total DCs and its subsets between initial-treatment group and retreatment group (P>0.05).


CONCLUSIONS
As its reflection of immune response to mycobacterium tuberculosis, DCs may be regarded as the indicator for screening reservoirs of MTB and evaluating anti-tuberculosis therapy.
Huang
Jia-yu
JY
Xiamen First Hospital Affiliated to Fujian Medical University, Department of Clinical Laboratory, Xiamen 361003, China. hjy831120117@hotmail.com
Song
Xiu-yu
XY
Hong
Qiang
Q
Ye
Zhi-zhong
ZZ
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000502:physiology*; D014376:Tuberculosis / Q000097:blood* / Q000276:immunology*; D055815:Young Adult