The bone formation capabilities of the anorganic bone matrix-synthetic cell-binding peptide 15 grafts in an animal periodontal model: a histologic and histomorphometric study in dogs.
BACKGROUND
The aim of this study is to verify the regenerative potential of particulate anorganic bone matrix-synthetic peptide-15 (ABM-P-15) in class III furcation defects associated or not with expanded polytetrafluoroethylene membranes.


METHODS
Class III furcation defects were produced in the mandibular premolars (P2, P3, and P4) of six dogs and filled with impression material. The membranes and the bone grafts were inserted into P3 and P4, which were randomized to form the test and control groups, respectively; P2 was the negative control group. The animals were sacrificed 3 months post-treatment.


RESULTS
Histologically, the complete closure of class III furcation defects was not observed in any of the groups. Partial periodontal regeneration with similar morphologic characteristics among the groups was observed, however, through the formation of new cementum, periodontal ligament, and bone above the notch. Histologic analysis showed granules from the bone graft surrounded by immature bone matrix and encircled by newly formed tissue in the test group. The new bone formation area found in the negative control group was 2.28 + or - 2.49 mm(2) and in the test group it was 6.52 + or - 5.69 mm(2), which showed statistically significant differences for these groups considering this parameter (Friedman test P <0.05). There was no statistically significant difference among the negative control, control, and test groups for the other parameters.


CONCLUSIONS
The regenerative potential of ABM-P-15 was demonstrated through new bone formation circumscribing and above the graft particles. The new bone also was accompanied by the formation of new cementum and periodontal ligament fibers.
Suaid
Flávia A
FA
Department of Oral Surgery and Periodontology, Ribeirão Preto School of Dentistry, University of São Paulo, Sao Paulo, Brazil.
Macedo
Guilherme O
GO
Novaes
Arthur B
AB
Borges
Germana J
GJ
Souza
Sérgio L S
SL
Taba
Mário
M
Palioto
Daniela B
DB
Grisi
Márcio F M
MF
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology*; D025021:Cementogenesis; D003739:Dental Cementum / Q000502:physiology; D004285:Dogs; D017823:Furcation Defects / Q000601:surgery*; D016557:Guided Tissue Regeneration, Periodontal; D008567:Membranes, Artificial; D023421:Models, Animal; D010513:Periodontal Ligament / Q000502:physiology; D011138:Polytetrafluoroethylene; D011897:Random Allocation; D012038:Regeneration / Q000187:drug effects
D018786:Bone Substitutes; D008567:Membranes, Artificial; C403137:PepGen P-15; D011138:Polytetrafluoroethylene