Na(+):aspartate coupling stoichiometry in the glutamate transporter homologue Glt(Ph).
The Na(+) aspartate symporter Glt(Ph) from Pyrococcus horikoshii is the only member of the glutamate transporter family for which crystal structures have been determined. The cation:aspartate coupling stoichiometry is unknown, thus hampering the elucidation of the ion coupling mechanism. Here we measure transport of (22)Na(+) and [(14)C]aspartate in proteoliposomes containing purified Glt(Ph) and demonstrate that three Na(+) ions are symported with aspartate.
Groeneveld
Maarten
M
Department of Biochemistry, University of Groningen, Groningen Biomolecular Science and Biotechnology Institute, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Slotboom
Dirk-Jan
DJ
D027322:Amino Acid Transport System X-AG / Q000737:chemistry*; D001224:Aspartic Acid / Q000737:chemistry* / Q000378:metabolism; D001692:Biological Transport; D011487:Protein Conformation; D011510:Proteolipids / Q000737:chemistry; D043902:Pyrococcus horikoshii / Q000737:chemistry; D012964:Sodium / Q000737:chemistry* / Q000378:metabolism
D027322:Amino Acid Transport System X-AG; D011510:Proteolipids; C020485:proteoliposomes; D001224:Aspartic Acid; D012964:Sodium