title: Evaluation of the protective effect of bovine lactoferrin against lipopolysaccharides in a bovine mammary epithelial cell line.
abstract: Lactoferrin (Lf) is a non-haem iron-binding glycoprotein with a molecular weight of about 80 kDa, synthesized by glandular epithelial cells and stored in the secondary granules of neutrophils. The physiological significance of Lf is related to non-specific immune defence against pathogens, immunomodulatory activity, iron homeostasis, antioxidant properties and regulation of cell growth. Lf is a bioactive component of the mammary secretions and its modulatory and defensive functions do affect the newborn and the mammary gland as well. In this work a bovine mammary epithelial cell line (BME-UV1) was used as an in vitro model of the bovine mammary epithelium to examine the protective role of exogenous bovine Lf (bLf) against the cytotoxic damage induced by bacterial lipopolysaccharides (LPS) and the endogenous bLf mRNA expression after LPS exposure. In the in vitro model used, exogenous bLf exerts a protective effect against endotoxin cytotoxicity, which could be mediated by the LPS-neutralizing capability of bLf. In addition, in BME-UV1 cells the response to LPS exposure does not involve bLf mRNA expression, suggesting that this cell line lack of functional LPS-responsive elements.
authors.affiliation: Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Università degli Studi di Milano, via Celoria 10, Milan, Italy. chiara.pecorini@gmail.com
authors.forename: Chiara
authors.initials: C
authors.lastname: Pecorini
authors.forename: Davide
authors.initials: D
authors.lastname: Sassera
authors.forename: Raffaella
authors.initials: R
authors.lastname: Rebucci
authors.forename: Francesca
authors.initials: F
authors.lastname: Saccone
authors.forename: Claudio
authors.initials: C
authors.lastname: Bandi
authors.forename: Antonella
authors.initials: A
authors.lastname: Baldi
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D020128:Inhibitory Concentration 50; D007781:Lactoferrin / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008321:Mammary Glands, Animal / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007781:Lactoferrin