Volatile compounds profile and sensory evaluation of Beninese condiments produced by inocula of Bacillus subtilis.
BACKGROUND
Three Beninese food condiments (ABS1(24h), IBS2(48h) and SBS3(48h)) were produced by controlled fermentation of African locust beans using inocula of pure cultures of Bacillus subtilis, BS1, BS2 and BS3, respectively. Quantitative and qualitative assessments of the volatile compounds in the condiments produced have been performed using the Likens-Nickerson simultaneous distillation-extraction method and GC-MS analysis, followed by a sensory evaluation in comparison with the spontaneously fermented condiments.


RESULTS
A total of 94 volatile compounds have been found including 53 compounds identified in relatively high concentrations and were subdivided into seven main groups with the predominance of four major groups: pyrazines, aldehydes, ketones and alcohols. Compared to the spontaneously fermented condiments, volatile compounds identified in controlled fermented condiments have been found in high number and in concentrations which varied according to the inoculum of B. subtilis used. The condiments produced with starter cultures scored significantly (P < 0.05) higher for odour than the spontaneously fermented condiments. But the overall acceptability (7/10) of the two types of condiments was similar.


CONCLUSIONS
The investigated B. subtilis, BS1, BS2 and BS3 can be considered as potential starter cultures for the fermentation of African locust beans to produce good quality of Beninese food condiments.
Azokpota
Paulin
P
Département de Nutrition et Sciences Alimentaires, Faculté des Sciences Agronomiques Université d'Abomey-Calavi, 01 BP 526, Cotonou, Bénin. azokpotap@yahoo.fr
Hounhouigan
Joseph D
JD
Annan
Nana T
NT
Odjo
Théophile
T
Nago
Mathurin C
MC
Jakobsen
Mogens
M
D001412:Bacillus subtilis / Q000378:metabolism*; D001541:Benin; D003212:Condiments / Q000032:analysis* / Q000382:microbiology; D057168:Distillation; D007887:Fabaceae / Q000378:metabolism* / Q000382:microbiology; D005285:Fermentation; D005516:Food Microbiology; D009812:Odorants; D010936:Plant Extracts / Q000737:chemistry*; D012639:Seeds / Q000378:metabolism* / Q000382:microbiology; D055549:Volatile Organic Compounds / Q000032:analysis*
D010936:Plant Extracts; D055549:Volatile Organic Compounds