IL-10 and IL-12 production in response to Mycobacterium tuberculosis total lipid antigens in multidrug resistant tuberculosis.
BACKGROUND
Mycobacterium tuberculosis lipid antigens take part in pathogenicity of the bacterium but the response of monocytes/macrophages to these antigens in tuberculosis is not well known.


OBJECTIVE
The aim of current investigation was to study the M. tuberculosis lipid antigens in tuberculosis pathogenesis.


METHODS
In the present study M. tuberculosis lipid antigens were extracted. Monocytes and macrophages from multidrug-resistant tuberculosis (MDR-TB), TB patients, asymptomatic healthy individuals with positive tuberculin skin test positive and healthy individuals with negative tuberculin skin test were collected using magnetic cell sorting. The cells were stimulated by M. tuberculosis total lipid antigens and IL-12 and IL-10 in their supernatants were measured by enzyme-linked immunosorbent assay.


RESULTS
The IL-12 production by monocytes in response to M. tuberculosis total sonicate antigens in the MDR-TB patients did not show a considerable difference with the PPD positive healthy subjects, whereas in the active TB patients, IL-12 levels significantly decreased (p<0.05). IL-10 production by monocytes in TB patients in response to total lipid antigens showed a significant increase in comparison to MDR-TB patients and healthy individuals.


CONCLUSIONS
In the MDR-TB patients, IL-10 and IL-12 production by monocytes in response to M. tuberculosis lipid antigens are similar to the healthy subjects.
Shams Shahemabadi
Ali
A
Department of Immunology, Shaheed Sadughi University of Medical Sciences, Yazd, Iran. alishams@ssu.ac.ir
Zavaran Hosseini
Ahmad
A
Shaghasempour
Shapour
S
Masjedi
Mohammad Reza
MR
Rayani
Majid
M
Shams
Majid
M
Esphandyari
Nasrin
N
Pouramiri
Majid
M
D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology*; D018664:Interleukin-12 / Q000096:biosynthesis / Q000276:immunology*; D008055:Lipids / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D009169:Mycobacterium tuberculosis; D014376:Tuberculosis / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology
D000942:Antigens, Bacterial; C508609:IL10 protein, human; D008055:Lipids; D016753:Interleukin-10; D018664:Interleukin-12