Exploring the concordance of Aspergillus fumigatus pathogenicity in mice and Toll-deficient flies.
The pathogenicity of six mutants of Aspergillus fumigatus that had been previously characterized in mice was assessed in Toll-deficient Drosophila melanogaster flies. Four out of six mutants of A. fumigatus, which displayed attenuated virulence in mice due to defects in siderophore biosynthesis (DeltasidA, DeltasidD), PABA metabolism (H515), and starvation stress response (DeltacpcA), also had attenuated virulence in the fly model. In addition, similarly to previous findings in the mouse model, DeltasidG mutant that is defective in extracellular siderophore biosynthesis retained full virulence in Toll-deficient flies. Overall, our studies reveal a high level of concordance between fly and murine models of invasive aspergillosis.
Chamilos
Georgios
G
The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA.
Bignell
Elaine M
EM
Schrettl
Markus
M
Lewis
Russell E
RE
Leventakos
Konstantinos
K
May
Gregory S
GS
Haas
Hubertus
H
Kontoyiannis
Dimitrios P
DP
D000818:Animals; D001232:Aspergillus fumigatus / Q000235:genetics / Q000472:pathogenicity*; D004195:Disease Models, Animal; D029721:Drosophila Proteins / Q000172:deficiency*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000382:microbiology*; D005656:Fungal Proteins / Q000235:genetics*; D051379:Mice; D051193:Toll-Like Receptors / Q000172:deficiency*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*
D029721:Drosophila Proteins; D005656:Fungal Proteins; C068721:Tl protein, Drosophila; D051193:Toll-Like Receptors; D037521:Virulence Factors