Nanoparticle enhanced surface plasmon resonance biosensing: application of gold nanorods.
In this report, we demonstrate the use of functionalized gold nanorods as amplification labels for ultra-sensitive surface plasmon resonance biosensing. Drastic sensitivity enhancement, owed to the electromagnetic interaction between the nanotag and the sensing film, was maximized using longitudinal plasmonic resonance of gold nanorods. The detection sensitivity of the nanorod-conjugated antibody is estimated to be approximately 40 pg/ml, which is 25 - 100 times more sensitive than the current reported values in the literature. This work paves the way to a new generation of ultra-sensitive nanoparticles-based biosensor platforms with maximized enhancement of sensitivity for ultra-fast screening and real-time detection of "hard-to-identify" biomolecules.
Law
Wing-Cheung
WC
Institute for Lasers, Photonics and Biophotonics, State University of New York at Buffalo, Buffalo, New York 14260-3000, USA.
Yong
Ken-Tye
KT
Baev
Alexander
A
Hu
Rui
R
Prasad
Paras N
PN
D055910:Antibodies, Immobilized / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D020342:Finite Element Analysis; D006046:Gold / Q000737:chemistry*; D007074:Immunoglobulin G / Q000032:analysis; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D055910:Antibodies, Immobilized; D007074:Immunoglobulin G; D006046:Gold