[Expression of survivin, caspase-3 in oral precancerous lesions and oral squamous-cell carcinoma].
OBJECTIVE
To investigate the roles of surviving and caspase-3 in the development of oral cancer.


METHODS
Archival tissue sections of 17 oral squamous cell carcinoma (OSCC), 28 oral leukoplakia with dysplasia, 10 normal oral mucosa were obtained from Capital Medical University School of Stomatology for immunohistochemical staining of markers of survivin and caspase-3. The cell apoptosis was detected with terminal deoxynucleotidyl transferase-mediated nucleotide shift enzyme (TdT) mediated d-UTP end labeling (TUNEL). Positively stained cells were counted and analyzed statistically to determine potential relationship between survivin, caspase-3 and cell apoptosis.


RESULTS
The expression of survivin was faint or negative in normal epithelial cells. The average positive rate of survivin was (1.05 ± 1.21)% in control group and (21.89 ± 10.45)% in OSCC. Caspase-3 was expressed in all the normal mucosa,but it obviously down-regulated in dysplasia and OSCC. The apoptosis index (AI) decreased from (0.89 ± 0.46)% in normal mucosa to (0.21 ± 0.12)% in OSCC.


CONCLUSIONS
Both survivin and caspase-3 are associated with carcinogenesis of the oral mucosa. Survivin may restrain cell apoptosis by inhibiting caspase-3.
Ding
Yan-ping
YP
Department of Oral Medicine, Capital Medical University School of Stomatology, Beijing 100051, China.
Li
Shu-xia
SX
Wu
Hong-ru
HR
Zhang
Xin-yan
XY
Tang
Xiao-fei
XF
Sun
Zheng
Z
D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D007972:Leukoplakia, Oral / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D009061:Mouth Mucosa / Q000201:enzymology / Q000378:metabolism; D009062:Mouth Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D011230:Precancerous Conditions / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D000077022:Survivin
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; C505407:CASP3 protein, human; D053148:Caspase 3