Histone deacetylase inhibitor promotes differentiation of embryonic stem cells into neural cells in adherent monoculture.
BACKGROUND
Embryonic stem (ES) cells poss unlimited self-renewal capacity and the ability to differentiate into cell of all three germ layers in vitro. Induced differentiation of ES cells to neural lineage cells has great potential in basic study of neurogenesis and regeneration therapy of neurodegenerative diseases. Histone deacetylase (HDAC) inhibitors enhance histone acetylation so that globularly activate gene expression and may initiate multilineage differentiation. In this study, we aimed to develop a method to induce the differentiation of ES cells to neural cells combining HDAC inhibition and neural cell selection.


METHODS
In this study, we used HDAC inhibitor sodium butyrate (NaB) to induce the differentiation of mouse embryonic stem cells to neural cells through monolayer culture. After differentiation initiation by histone deacetylase inhibitor sodium butyrate, neural cells were induced and selected with a serum free culture system.


RESULTS
Homogeneous neurons without glial cells demonstrated by molecular marker expression were differentiated with the method. The resultant neurons were excitable.


CONCLUSIONS
The method combined differentiation induction effect of HDAC inhibitors and selective culture system to derive neural cells from ES cells, and implied the involvement of epigenetic regulation in neural differentiation.
Yao
Xing
X
Department of Hepatopancreatobilary Surgery, Huzhou Central Hospital, Huzhou, Zhejiang 313000, China. yaoy@mail.huptt.zj.cn
Zhang
Jia-rong
JR
Huang
Hua-rong
HR
Dai
Li-cheng
LC
Liu
Qing-jun
QJ
Zhang
Ming
M
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D002448:Cell Adhesion; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000502:physiology
D002087:Butyrates; D056572:Histone Deacetylase Inhibitors; D016222:Fibroblast Growth Factor 2