A small interfering ABCE1-targeting RNA inhibits the proliferation and invasiveness of small cell lung cancer.
Small cell lung cancer (SCLC) is a highly aggressive lung neoplasm. To study the pathogenesis of SCLC, we investigated roles of ABCE1, a member of the ATP-binding cassette (ABC) superfamily, in the development of small cell lung cancer. RNA interference was used to knock down ABCE1 expression in human small cell lung cancer cell lines (NCI-H446). Then we examined the effects of ABCE1 knockdown in cancer cells, including proliferation, invasiveness, apoptosis, and gene expression. We found that ABCE1 could be efficiently knocked down by siRNA, and the ABCE1 silence inhibited the proliferation, invasiveness of small cell lung cancer cell lines NCI-H446. In conclusion, our results suggest that ABCE1 play an important role in the pathogenesis of human small cell lung cancer cell. ABCE1 may be used as a potential target of gene therapy for small cell lung cancer in future.
Huang
Bo
B
Department of Thoracic Surgery, the Fourth Affiliated Hospital of China Medical University, Liaoning Shenyan 110032, PR China.
Gao
Ying
Y
Tian
Dali
D
Zheng
Maogen
M
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D008969:Molecular Sequence Data; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D055752:Small Cell Lung Carcinoma / Q000235:genetics / Q000473:pathology
C501917:ABCE1 protein, human; D018528:ATP-Binding Cassette Transporters; D034741:RNA, Small Interfering