title: Silver-coated silica beads applicable as core materials of dual-tagging sensors operating via SERS and MEF.
abstract: We have developed dual-tagging sensors, operating via both surface-enhanced Raman scattering (SERS) and metal-enhanced fluorescence (MEF), composed of silver-coated silica beads onto which were deposited SERS markers and dye-grafted polyelectrolytes, for multiplex immunoassays. Initially, a very simple electroless-plating method was applied to prepare Ag-coated silica beads. The Raman markers were then assembled onto the Ag-coated silica beads, after which they were brought to stabilization by the layer-by-layer deposition of anionic and cationic polyelectrolytes including a dye-grafted polyelectrolyte. In the final stage, the dual-tagging sensors were assembled onto them with specific antibodies (antihuman-IgG or antirabbit-IgG) to detect target antigens (human-IgG or rabbit-IgG). The MEF signal was used as an immediate indicator of molecular recognition, while the SERS signals were subsequently used as the signature of specific molecular interactions. For this reason, these materials should find wide application, especially in the areas of biological sensing and recognition that rely heavily on optical and spectroscopic properties.
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 151-742, Korea. kwankim@snu.ac.kr
authors.forename: Kwan
authors.initials: K
authors.lastname: Kim
authors.forename: Yoon Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Hyang Bong
authors.initials: HB
authors.lastname: Lee
authors.forename: Kuan Soo
authors.initials: KS
authors.lastname: Shin
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000378:metabolism; D008863:Microspheres; D011817:Rabbits; D012822:Silicon Dioxide / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D012834:Silver; D012822:Silicon Dioxide