title: [The polymorphism of cytochrome P(450) 2C19 gene in patients with invasive fungal infections.].
abstract: OBJECTIVE
  To investigate the polymorphism profile of cytochrome P(450) 2C19 (CYP2C19) in Chinese patients with invasive fungal infections.
  
  
  METHODS
  Two major single nucleotide polymorphism loci of the CYP2C19 gene (CYP2C19*2 and CYP2C19*3) were genotyped with PCR and restriction fragment length polymorphism (PCR-RFLP) in 134 patients with invasive fungal infections and 134 healthy volunteers. Allele frequencies and the proportions of metabolizer phenotypes were compared.
  
  
  RESULTS
  In patients with invasive fungal infections, CYP2C19*1, CYP2C19*2 and CYP2C19*3 alleles showed frequencies of 58.2%, 36.6% and 5.2%. In healthy volunteers, the frequencies of CYP2C19*1, CYP2C19*2 and CYP2C19*3 were 63.4%, 34.3% and 2.2%. There was no significant difference in allele frequencies between the two groups. Of the patients with invasive fungal infections, 33.6% were homozygous extensive metabolizers, 50.0% heterozygous extensive metabolizers and 16.4% poor metabolizers. Of the healthy volunteers, 40.3% were homozygous extensive metabolizers, 48.5% heterozygous extensive metabolizers and 11.2% poor metabolizers. The proportions of metabolizer phenotypes were similar between the two groups.
  
  
  CONCLUSIONS
  Significant CYP2C19 polymorphism was detected in both groups. Approximately two thirds of the Chinese patients were either heterozygous extensive metabolizers or poor metabolizers. The genetic polymorphism may have important effect on drug metabolism in these patients.
authors.affiliation: Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China. Email: zhulp@fudan.edu.cn.
authors.forename: Ji-Qin
authors.initials: JQ
authors.lastname: Wu
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zhu
authors.forename: Xue-Ting
authors.initials: XT
authors.lastname: Ou
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Qiang-Qiang
authors.initials: QQ
authors.lastname: Zhang
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Weng
mesh_terms: D000483:Alleles; D001189:Aryl Hydrocarbon Hydroxylases; D065731:Cytochrome P-450 CYP2C19 / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D011110:Polymorphism, Genetic
chemical_list: D001189:Aryl Hydrocarbon Hydroxylases; D065731:Cytochrome P-450 CYP2C19