Fluorescent differential display analysis of Lactobacillus sakei strains under stress conditions.
Lactobacillus (Lb.) sakei is widely used as starter in the production process of Italian fermented sausages and its growth and survival are affected by various factors such as temperature, pH and salt concentration. We studied the behaviour of Lb. sakei strains under various growth conditions relative to acid, osmotic and heat stress treatments by a novel fluorescent differential display (FDD) technique. This study obtained the development and the optimization of a technique that allows the identification of genome expression changes, associated with differential microbial behaviour under different stress conditions with a better stress response definition and a better discrimination of starter cultures. DNA sequence information from the FDD products provided an important tool to assess and observe the response to a variety of environmental stimuli and the adaptation to bacterial stress. Our work provided an innovative FDD method, with a high level of reproducibility and quality for studying and probing the knowledge of the relation between differential genome expression and different stresses tolerance.
Bonomo
Maria Grazia
MG
Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano,10, 85100 Potenza, Italy. mariagrazia.bonomo@unibas.it
Sico
Maria Anna
MA
Grieco
Simona
S
Salzano
Giovanni
G
D017931:DNA Primers / Q000235:genetics; D005456:Fluorescent Dyes / Q000032:analysis; D020869:Gene Expression Profiling / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial; D006358:Hot Temperature; D007778:Lactobacillus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D013312:Stress, Physiological
D017931:DNA Primers; D005456:Fluorescent Dyes