title: [Detecting penehyclidine hydrochloride in rats plasma and tissues with HPLC-MS/MS].
abstract: OBJECTIVE
  To develop a method for detecting penehyclidine hydrochloride (PH) in mouse plasma and tissues using HPLC-MS/MS.
  
  
  METHODS
  The plasma and tissue samples were extracted with petroleum ether, ethyl ether (70:30). The isolation of PH was achieved on an Allure C18, (50 x 2.1 mm, 5 microm) column, with methanol/5 mmol/L ammonium acetate/triethylamine = 90/10/0.05 (adjusted pH 5.8 by formic acid as mobile phase. Benzhydramine was used as the internal standard.
  
  
  RESULTS
  The calibration curves were linear over the range of 0.39-200 ng/mL for plasma and 0.391-100 ng/mL for tissues. The extraction recovered 67.32-70.80% of PH in plasma, 84.99%-89.27% of PH in lung tissues, and 76.86%-81.98% of PH in brain tissues. The HPLC detected 97.66%-99.97% of PH in extracted samples of plasma, 96.55%-101.65% extracted samples of lung and 95.18%-100.21% of extracted samples of brain. The within-day RSD were 1.94%-2.93%, 1.73%-3.70% and 2.35%-2.79% for plasma, lung, and brain, respectively. The between-day RSD were 3.00%-3.85% 2.86%-3.94% and 2.77%-5.00% for plasma, lung, and brain, respectively. Both the long-term and freeze-thaw stabilities were acceptable. The long-term RSD were 1.52%-5.26%, 1.88%-2.93%, and 2.22%-3.76% for plasma, lung, and brain, respectively. The freeze-thaw RSD were 0.38%-3.55%, 2.79%-9.60%, and 1.35%-2.29% for plasma, lung, and brain, respectively.
  
  
  CONCLUSIONS
  The assay is simple and accurate, with good reproducibility, and can satisfy to the needs of pharmacokinetics and relative bioavailability studies on penehyclidine hydrochloride.
authors.affiliation: Laboratory of Clinical Pharmacology, GCP Center, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.forename: Mao-Zhi
authors.initials: MZ
authors.lastname: Liang
authors.forename: Feng
authors.initials: F
authors.lastname: Nan
authors.forename: Jin
authors.initials: J
authors.lastname: Xiang
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Qin
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011812:Quinuclidines / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D011812:Quinuclidines; C486140:penehyclidine