Membrane proteome of the green sulfur bacterium Chlorobium tepidum (syn. Chlorobaculum tepidum) analyzed by gel-based and gel-free methods.
Chlorobium tepidum is a Gram-negative bacterium of the green sulfur phylum (Chlorobia). Chlorobia are obligate anaerobic photolithoautotrophs that are widely distributed in aquatic environments where anoxic layers containing reduced sulfur compounds are exposed to light. The envelope of C. tepidum is a complex organelle composed of the outer membrane, the periplasm-peptidoglycan layer, and the cytoplasmic membrane. In addition to the outer and plasma membranes, C. tepidum contains chlorosomes attached to the cytoplasmic side of the plasma membrane. Each cellular compartment has a unique set of proteins, called sub-proteome. An important aim of proteome analysis is to study the level of the expressed genes and their response to environmental changes. Membrane protein studies are of primary importance to understand how nutrients are transported inside the cell, how toxic molecules are exported, and the mechanisms of photosynthesis and energy metabolism.
Kouyianou
Kalliopi
K
Division of Biochemistry, Department of Chemistry, University of Crete, Voutes, Heraklion, Greece.
Aivaliotis
Michalis
M
Gevaert
Kris
K
Karas
Michael
M
Tsiotis
Georgios
G
D001426:Bacterial Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000378:metabolism*; D041883:Chlorobium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008565:Membrane Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012995:Solubility / Q000187:drug effects
D001426:Bacterial Proteins; D003902:Detergents; D008565:Membrane Proteins; D020543:Proteome