[Study of hepatic energy metabolism on rats by six cold property traditional Chinese medicines].
OBJECTIVE
To investigate the common rule hepatic energy metabolism on rats by six cold property of traditional Chinese medicines (TCM).


METHODS
The activities of Na+ - K+ - ATPase, Ca2+ - ATPase and SDH, the content of hepatic glycogen, and the mRNA expression of hepatic uncoupling protein 2 (UCP2) were measured after the rats and been administrated with water extracts of Radix Sophorae Flavescentis, Fructus Gardeniae, Cortex Phellodendri, Radix Scutellariae, Rhizoma Coptidis, Radix Gentianae respectively at 6.0, 7.0, 8.4, 6.0, 7.0, 4.0 g x kg(-1) doses for 30 days, twice a day.


RESULTS
The activities of Na+ - K+ - ATPase, Ca2+ - ATPase and SDH were depressed significantly by six cold property TCM. The decreased tendency of SDH was observed by six cold property of TCM, while statistical significance was obtained in Cortex Phellodendri, Radix Scutellariae, Rhizoma Coptidis, Radix Gentianae. The increased tendency of hepatic glycogen content was found by six cold property TCM, while statistical significance was not obtained in six groups. The decreased tendency of the mRNA expression of UCP2 was found by six cold property TCM, while statistical significance was obtained in Radix Sophorae Flavescentis, Cortex Phellodendri, and Radix Scutellariae groups.


CONCLUSIONS
The six cold property TCM have the good effects on hepatic energy metabolism by decreasing the activity of mitochondria SDH to reduce the production of ATP by decreasing the activities of Na+ - K+ - ATPase, Ca2+ - ATPase to cut down the consumption of ATP, by decreasing the mRNA expression of hepatic UCP2 to decrease the heat production.
Huang
Liping
L
Beijing University of Chinese Medicine, Beijing 100102, China.
Peng
Shuhong
S
Meng
Xiaofang
X
Hu
Qiang
Q
Zhang
Su
S
Yu
Riyue
R
Liu
Hongning
H
Sun
Jianning
J
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D000089042:Coptis chinensis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects*; D032062:Gardenia / Q000737:chemistry; D029965:Gentianaceae / Q000737:chemistry; D007473:Ion Channels / Q000235:genetics; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D031364:Scutellaria baicalensis / Q000737:chemistry; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D000071246:Uncoupling Protein 2
D004365:Drugs, Chinese Herbal; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2; D000255:Adenosine Triphosphate; C107393:Coptidis rhizoma extract; D013385:Succinate Dehydrogenase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase