A new approach to fluorescence "turn-on" sensing of alpha-amino acids.
For the first time, a conjugated fluorescent polymer was utilized to probe alpha-amino acids, sensitively and selectively, through a new approach. First the strong fluorescence of the prepared polyfluorene (P1) was quenched by trace copper ions, and then the quenched fluorescence was recovered upon the addition of alpha-amino acids, making P1 a new and sensitive biosensor toward alpha-amino acids. The experimental results demonstrated that the alpha-amino acid selective nature of P1 over other analytes was relatively good. Thus, the work reported here might open up a new avenue for developing new biosensors.
Li
Zhong'an
Z
Lou
Xiaoding
X
Li
Zhen
Z
Qin
Jingui
J
D000596:Amino Acids / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000596:Amino Acids; D005449:Fluorenes; D005456:Fluorescent Dyes; D011108:Polymers; D003300:Copper