[On-chip Cellomics technology for drug screening system using cardiomyocyte cells from human stem cell].
Limitation of conventional human Ether-a-go-go Related Gene (hERG) assay and QT prolongation testing for accurate prediction of Torsades de Pointes (TdP) by compounds showed us the necessity of a new approach to evaluate global cardiac safety. As one of the advanced applications of an on-chip cellomics system, on-chip cardiomyocyte cell network-based re-entry model assay has the potential to measure the TdP probability as a pre-clinical test for cardiac safety. This system also can estimate the heart pressure, Na, K, and Ca ion channel conditions using a single cell-based optical/electrical measurement system. In this presentation, we present the system setup and then its possible application for drug discovery and toxicology.
Yasuda
Kenji
K
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan. yasuda.bmi@tmd.ac.jp
D018929:Cell Culture Techniques / Q000379:methods*; D002468:Cell Physiological Phenomena; D003584:Cytological Techniques / Q000379:methods*; D055808:Drug Discovery; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D032383:Myocytes, Cardiac; D020411:Oligonucleotide Array Sequence Analysis; D013234:Stem Cells; D016171:Torsades de Pointes