[Regulating effects of aquaporin 4 expression in LoVo cells by serum containing total anthraquinone in rheum].
OBJECTIVE
To investigate the regulating effects of serum containing total anthraquinone in rheum on the expression of AQP4 in cultured LoVo cells in vitro.


METHODS
Sixteen SD rats were randomly divided into 4 groups respectively treated with 0, 1.4, 2.5, 4.5 g x kg(-1) x d(-1) of total anthraquinone in rheum by intragastric administration. After 7 days of treatment, the rats were anesthetized and celiotomized to prepare the serum containing total anthraquinone in rheum. LoVo cells cultured in vitro were treated with different concentrations of total anthraquinone in rheum for 24 h. The expression levels of protein and mRNA of AQP4 IN LoVo cells were decided by Western blot and semiquantive RT-PCR.


RESULTS
Western blot and semiquantive RT-PCR showed that serum containing total anthraquinone in rheum prepared from rats treated with 4.5 g x kg(-1) x d(-1) of total anthraquinone in rheum could inhibit the expression of mRNA and protein levels of AQP 4 in LoVo cells (P<0.01).


CONCLUSIONS
Serum containing total anthraquinone in rheum can inhibit both the transcription and translation of AQP4 gene, indicating that the regulation mechanism of rhubarb on the expression of AQP4 can be related to the cathartic effect of rhubarb.
Liu
Qing
Q
Fourth Military Medical University PLA Centre of Traditional Chinese Medicine, Xijing Hospital, Xi'an 710032, China.
Li
Feng
F
Li
Junchang
J
Yao
Jufeng
J
Wang
Xin
X
Wang
Changhai
C
Wang
Wen
W
D000818:Animals; D000880:Anthraquinones / Q000097:blood* / Q000494:pharmacology*; D051401:Aquaporin 4 / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012250:Rheum / Q000737:chemistry*
D000880:Anthraquinones; D051401:Aquaporin 4