[Stabilization of peroxidase conjugates used in enzyme immunoassay systems to detect Ebola and Marburg virus antigens].
The time course of changes in the activity of solutions of horseradish peroxidase conjugates with immunoglobulins against Ebola and Marburg fevers was studied in the presence of different components. The series of the conjugates of ELISA kits for the detection of Ebola and Marburg virus antigens, which were prepared on the basis of the designed stabilizing solution, preserved at less than 90% of its baseline activity during 10 months at a storage temperature of 2 to 8 degrees C.
Pirozhkov
A P
AP
Borisevich
I V
IV
Snetkova
O Iu
OIu
Androshchuk
I A
IA
Syromiatnikova
S I
SI
Khmelev
A L
AL
Shatokhina
I V
IV
Kudrin
V Iu
VIu
Timofeev
M A
MA
Pantiukhov
V B
VB
Borisevich
S V
SV
Markov
V I
VI
Bondarev
V P
VP
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification*; D029043:Ebolavirus / Q000276:immunology / Q000302:isolation & purification*; D004795:Enzyme Stability; D004797:Enzyme-Linked Immunosorbent Assay; D019142:Hemorrhagic Fever, Ebola; D006735:Horseradish Peroxidase / Q000737:chemistry*; D007136:Immunoglobulins / Q000737:chemistry / Q000276:immunology; D008379:Marburg Virus Disease / Q000175:diagnosis*; D029024:Marburgvirus / Q000276:immunology / Q000302:isolation & purification*
D000956:Antigens, Viral; D007136:Immunoglobulins; D006735:Horseradish Peroxidase