Aqueous synthesis and fluorescence-imaging application of CdTe/ZnSe core/shell quantum dots with high stability and low cytotoxicity.
CdTe/ZnSe core/shell quantum dots were directly synthesized in an aqueous condition by heating a mixed solution of ZnCI2, NaHSe and CdTe QDs in the presence of mercaptosuccinic acid as a stabilizer. By controlling the size and composition, the CdTe/ZnSe QDs with emission wavelength ranging from 540 to 630 nm, high quantum yield (44%) and narrow full width at half maximum (FWHM) could be obtained. Characterization results with HRTEM, XRD and EDX have shown that the synthesized CdTe/ZnSe QDs have good monodispersity and a nice crystal structure, and exhibited better stability and less cytotoxicity as compared with CdTe QDs. Furthermore, luminescent QD-IgG bioprobes were produced to detect the breast cancer marker Her2 on the surface of fixed MCF-7 cancer cells for their optical imaging.
Fu
Tao
T
Centre for Optical and Electromagnetic Research, State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310058, China.
Qin
Hai-Yan
HY
Hu
Hua-Jun
HJ
Hong
Zhi
Z
He
Sailing
S
D001943:Breast Neoplasms / Q000737:chemistry / Q000473:pathology; D019187:Cadmium Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D005453:Fluorescence; D006801:Humans; D046529:Microscopy, Electron, Transmission; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D013691:Tellurium / Q000737:chemistry*; D014867:Water; D014961:X-Ray Diffraction; D017967:Zinc Compounds / Q000737:chemistry*
D019187:Cadmium Compounds; D018036:Selenium Compounds; D017967:Zinc Compounds; D014867:Water; D013691:Tellurium; C044696:zinc selenide; C028337:cadmium telluride