title: Bioelectrical impedance may predict cell viability during ischemia and reperfusion in rat liver.
abstract: Ischemia and reperfusion (I/R) injury is a major cause of hepatic failure after liver surgery, but no method could monitor or predict it real-time during surgery. We measured bioelectrical impedance (BEI) and cell viability to assess the usefulness of BEI during I/R in rat liver. A 70% partial liver ischemia model was used. BEI was measured at various frequencies. Adenosine triphosphate (ATP) content, and palmitic acid oxidation rate were measured, and histological changes were observed in order to quantify liver cell viability. BEI changed significantly during ischemia at low frequency. In the ischemia group, BEI increased gradually during 60 min of ischemia and had a tendency to plateau thereafter. The ATP content decreased below 20% of the baseline level. In the I/R group, BEI recovered to near baseline level. After 24 hr of reperfusion, the ATP contents decreased to below 50% in 30, 60 and 120 min of ischemia and the palmitic acid metabolic rates decreased to 91%, 78%, and 74%, respectively, compared with normal liver. BEI may be a good tool for monitoring I/R during liver surgery. The liver is relatively tolerant to ischemia, however after reperfusion, liver cells may be damaged depending upon the duration of ischemia.
authors.affiliation: Research Institute of Biomedical Engineering, College of Medicine, Yeungnam University, Daegu, Korea.
authors.forename: Mei Lan
authors.initials: ML
authors.lastname: Cui
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Ahn
authors.forename: Jong Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Hyoun Jin
authors.initials: HJ
authors.lastname: Shin
authors.forename: Dong Shik
authors.initials: DS
authors.lastname: Lee
authors.forename: Hong Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Sung Su
authors.initials: SS
authors.lastname: Yun
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002470:Cell Survival; D017097:Electric Impedance; D004734:Energy Metabolism; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010168:Palmitates / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology
chemical_list: D010168:Palmitates; D000255:Adenosine Triphosphate