A new phosphorimetry for the determination of trace alkaline phosphatase using multi-wall carbon nanotubes and its diagnosis of human diseases.
Multi-wall carbon nanotubes (MWNTs) could be modified as water soluble MWNTs (it was called as MWNTs-A and MWNTs-B) by chemical methods. MWNTs-A and MWNTs-B could emit room temperature phosphorescence (RTP) signal on the surface of nitrocellulose membrane (NCM). A new solid substrate-room temperature phosphorimetry (SS-RTP) for the determination of trace alkaline phosphatase (ALP) was established based on the signal magnification effect of tween-80 and ALP on MWNTs-B's RTP intensity and the linear relationship between the content of ALP and the DeltaI(P) of the system. The linear range of this method was 0.0020-0.80 (fg spot(-1), sample volume: 0.40 microl spot(-1)), the regression equation of working curve was DeltaI(P) = 0.8170 + 96.84m(ALP) (fg spot(-1)), correlation coefficient (r) was 0.9986. This method had high sensitivity (detection limit (LD): 1.4 ag spot(-1)), good selectivity (Er < or = +/- 5 in-care-of, coexistence species were of no interference), high precision (RSDs were 4.4%-1.2%) and accuracy. It was applied to the determination of trace ALP in human serum and the diagnosis of human diseases. The results were tallied with those of enzyme-linked immunosorbent assay (ELISA). The mechanism of new SS-RTP for the determination of trace ALP was discussed, which laid the theory foundation for the analytical application of MWNTs in life science.
Liu
Jia-Ming
JM
Department of Chemistry and Environmental Science, Zhangzhou Normal Colleage, Zhangzhou, 363000, People's Republic of China. zzsyliujiaming@163.com
Rao
Ying
Y
Chen
Li
L
Wang
Xin-Xing
XX
Lin
Li-Ping
LP
Lin
Chang-Qing
CQ
Zhang
Li-Hong
LH
Ou-Yang
Ying
Y
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000097:blood; D003933:Diagnosis; D006801:Humans; D006813:Humidity; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D017942:Nitric Acid / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D013464:Sulfuric Acids / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D007202:Indicators and Reagents; D037742:Nanotubes, Carbon; D013464:Sulfuric Acids; D017942:Nitric Acid; D006861:Hydrogen Peroxide; D000469:Alkaline Phosphatase; C033158:sulfuric acid; D010100:Oxygen