Optimization of fibrinogen isolation for manufacturing autologous fibrin glue for use as scaffold in tissue engineering.
Numerous manufacturing techniques for autogenous fibrin glue used as scaffold material have been described. As there is no consensus regarding the influence of chemical additives on cell biology, it was the aim of this study to establish a method for manufacturing autologous fibrin glue without any additives. The serum part was separated from whole blood. After fibrinogen precipitation, centrifugation was performed to obtain the fibrinogen pellet. Various experimental series were run to examine influences of various temperatures or substituting centrifugation for sedimentation. The method as described here is effective, simple, and performed without any additives, which could potentially influence cell biology.
Froelich
K
K
Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Surgery, University of Würzburg, Germany. froelich_k@klinik.uni-wuerzburg.de
Pueschel
R C
RC
Birner
M
M
Kindermann
J
J
Hackenberg
S
S
Kleinsasser
N H
NH
Hagen
R
R
Staudenmaier
R
R
D007611:Aprotinin / Q000737:chemistry*; D002356:Cartilage / Q000737:chemistry; D002498:Centrifugation; D015718:Fibrin Tissue Adhesive / Q000737:chemistry; D005340:Fibrinogen / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D006801:Humans; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D015718:Fibrin Tissue Adhesive; D005782:Gels; D005340:Fibrinogen; D007611:Aprotinin