Electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells.
It has been reported previously that electric pulses of sufficiently high voltage and short duration can permeabilize the membranes of various organelles inside living cells. In this article, we describe electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells. The cells were exposed to short, high-voltage electric pulses (from 1 to 20 pulses, 60 ns, 50 kV/cm, repetition frequency 1 kHz). We observed that 10 and 20 such pulses induced permeabilization of membranes of endocytotic vesicles, detected by release of lucifer yellow from the vesicles into the cytosol. Simultaneously, we detected uptake of propidium iodide through plasma membrane in the same cells. With higher number of pulses permeabilization of the membranes of endocytotic vesicles by pulses of given parameters is accompanied by permeabilization of plasma membrane. However, with lower number of pulses only permeabilization of the plasma membrane was detected.
Napotnik
Tina Batista
TB
Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, 1000, Ljubljana, Slovenia.
Rebersek
Matej
M
Kotnik
Tadej
T
Lebrasseur
Eric
E
Cabodevila
Gonzalo
G
Miklavcic
Damijan
D
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000502:physiology; D018274:Electroporation / Q000379:methods*; D007546:Isoquinolines / Q000493:pharmacokinetics; D008546:Melanoma, Experimental / Q000378:metabolism*; D051379:Mice; D011419:Propidium / Q000493:pharmacokinetics; D022161:Transport Vesicles / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D007546:Isoquinolines; D011419:Propidium; C017475:lucifer yellow