A novel amperometric biosensor based on banana peel (Musa cavendish) tissue homogenate for determination of phenolic compounds.
In this study the biosensor was constructed by immobilizing tissue homogenate of banana peel onto a glassy carbon electrode surface. Effects of immobilization materials amounts, effects of pH, buffer concentration and temperature on biosensor response were studied. In addition, the detection ranges of 13 phenolic compounds were obtained with the help of the calibration graphs. Storage stability, repeatability of the biosensor, inhibitory effect and sample applications were also investigated. A typical calibration curve for the sensor revealed a linear range of 10-80 microM catechol. In reproducibility studies, variation coefficient and standard deviation were calculated as 2.69%, 1.44 x 10(-3) microM, respectively.
Ozcan
Hakki Mevlut
HM
Trakya University, Faculty of Science, Department of Chemistry, Edirne, Turkey. ozcanhakki@gmail.com
Sagiroglu
Ayten
A
D001628:Beverages / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D002021:Buffers; D002138:Calibration; D004156:Catechol Oxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D004563:Electrochemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D008670:Metals / Q000494:pharmacology; D028521:Musa / Q000201:enzymology; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D019441:Plant Epidermis / Q000201:enzymology*; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D013696:Temperature
D002021:Buffers; D004791:Enzyme Inhibitors; D008670:Metals; D010636:Phenols; D010936:Plant Extracts; D005780:Gelatin; D004156:Catechol Oxidase; D005976:Glutaral