title: Is HPV-DNA testing a useful tool in predicting low-grade squamous intraepithelial lesion outcome? A retrospective longitudinal study.
abstract: HPV-DNA testing has entered in clinical practice. Three important questions remain controversial: 1) which is the best HPV-DNA technology? 2) Which age group should be targeted? 3) Is HPV-DNA testing predictive of disease outcome? The answers to these queries represent the endpoints of this study. The population of this retrospective study consisted of 272 women, each one having: baseline cytological diagnosis of Low-grade Squamous Intraepithelial Lesion (LSIL); baseline HPV-DNA reports by Hybrid Capture 2 (HC2) and MY09/11 consensus primers PCR; follow-up duration over 3-years; cytological report of disease status at follow-up time. Firstly, we assessed the concordance and the performances of both HPV-DNA testing, then we correlated, respectively HPV-DNA results and age of patients to disease outcome. DNA testing methods agreed in 83.4 percent of cases (K=0.66). Baseline HPV-DNA result was not significantly associated to disease outcome (p=0.06). Within HPV-DNA positive group, we found no evidence of correlation between age and LSIL prognosis (p=0.89). Confining the analysis to age-stratified HPV-DNA negative women, the differences were statistically significant (p=0.01). In conclusion, HPVDNA testing gives no information about the real behaviour of cervical abnormalities. These findings suggest the demand for additive markers, reflecting the risk of progression, in prevention strategy and clinical approach.
authors.affiliation: Department of Oncology and Neurosciences, Section of Cytopathology, G. dAnnunzio University of Chieti-Pescara, Italy. zappacosta2@hotmail.com
authors.forename: R
authors.initials: R
authors.lastname: Zappacosta
authors.forename: D
authors.initials: D
authors.lastname: Caraceni
authors.forename: L
authors.initials: L
authors.lastname: Ciccocioppo
authors.forename: M
authors.initials: M
authors.lastname: Ottaviantonio
authors.forename: F
authors.initials: F
authors.lastname: Conti
authors.forename: S
authors.initials: S
authors.lastname: Andreozzi
authors.forename: F
authors.initials: F
authors.lastname: Petrucci
authors.forename: S
authors.initials: S
authors.lastname: Rosini
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D002584:Cervix Uteri / Q000473:pathology* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D004847:Epithelial Cells / Q000473:pathology / Q000821:virology; D005260:Female; D006801:Humans; D008137:Longitudinal Studies; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D014626:Vaginal Smears
chemical_list: D004279:DNA, Viral