Characterization of Bombyx mori nucleopolyhedrovirus ORF109 that encodes a 25-kDa structural protein of the occlusion-derived virion.
Bombyx mori nucleopolyhedrovirus (BmNPV) ORF109 (Bm109) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm109 was characterized. The transcript of Bm109 was detected at 12-72 h postinfection (p.i.). Polyclonal antiserum raised to a His-BM109 fusion protein recognized BM109 in infected cell lysates from 24 to 72 h p.i., suggesting that Bm109 is a late gene. Localization of the BM109 in BV and ODV by western analyses demonstrated that BM109 was proteins of ODV.
Zhou
Yang
Y
Institute of Life Sciences, Jiangsu University, Zhenjiang, People's Republic of China. zhouyangzhy@sohu.com
Yao
Qin
Q
Shen
Hongxing
H
Xia
Hengchuan
H
Lin
Feng
F
Chen
Keping
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005784:Gene Amplification; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D015678:Viral Structural Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*
D004279:DNA, Viral; D015678:Viral Structural Proteins