[Identification of corium stomachium galli by molecular taxonomy].
OBJECTIVE
To establish a convenient and accurate method of DNA molecular marker for the identification of corium stomachium galli.


METHODS
Cytb mtDNA sequences of Gallus gallus domestica and three other species of poultry were downloaded from Genbank. Species-specific PCR primers were designed according to the differential DNA fragments of the cytb genes. PCR tests were performed with the DNAs extracted from G. gallus domestica, three other poultry species and domestic mammal animals.


RESULTS
The specific primers of G. gallus domestica could only amplify the cytb mtDNA of G. gallus domestica.


CONCLUSIONS
The primers are specific to G. gallus domestica mtDNA and can used to discern Corium stomachium from the false medicine.
Qu
Meng
M
Medical Department of Beihua University, Jilin 132013, China. dzhqm@dzh.com.cn
Cui
Jichun
J
Dong
Zhiheng
Z
Zhang
Lihua
L
Wang
Bingmei
B
Zhang
Jilin
J
Li
Cai
C
D000818:Animals; D002645:Chickens / Q000145:classification* / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D008099:Liver / Q000378:metabolism; D016133:Polymerase Chain Reaction
D004272:DNA, Mitochondrial