Fission yeast Rad26ATRIP delays spindle-pole-body separation following interphase microtubule damage.
The conserved fission yeast protein Rad26(ATRIP) preserves genomic stability by occupying central positions within DNA-structure checkpoint pathways. It is also required for proper cellular morphology, chromosome stability and following treatment with microtubule poisons. Here, we report that mutation of a putative nuclear export sequence in Rad26(ATRIP) disrupted its cytoplasmic localization in untreated cells and conferred abnormal cellular morphology, minichromosome instability and sensitivity to microtubule poisons without affecting DNA-structure checkpoint signaling. This mutation also disrupted a delay to spindle-pole-body separation that occurred following microtubule damage in G(2). Together, these results demonstrate that Rad26(ATRIP) participates in two genetically defined checkpoint pathways--one that responds to genomic damage and the other to microtubule damage. This response to microtubule damage delays spindle-pole-body separation and, in doing so, might preserve both cellular morphology and chromosome stability.
Herring
Matthew
M
Department of Biology, University of Colorado at Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USA.
Davenport
Nick
N
Stephan
Kendra
K
Campbell
Shawna
S
White
Rebecca
R
Kark
Jonathan
J
Wolkow
Tom D
TD
D000483:Alleles; D000595:Amino Acid Sequence; D001562:Benzimidazoles / Q000494:pharmacology; D002219:Carbamates / Q000494:pharmacology; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D043171:Chromosomal Instability / Q000187:drug effects; D004249:DNA Damage; D004271:DNA, Fungal / Q000378:metabolism; D016195:G2 Phase / Q000187:drug effects; D007399:Interphase / Q000187:drug effects; D064246:Mad2 Proteins; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis / Q000187:drug effects; D008969:Molecular Sequence Data; D049790:Nuclear Export Signals; D009687:Nuclear Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D008941:Spindle Apparatus / Q000187:drug effects / Q000378:metabolism*
D001562:Benzimidazoles; D002219:Carbamates; D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D064246:Mad2 Proteins; D049790:Nuclear Export Signals; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; C577696:mad2 protein, S pombe; C088086:rad26 protein, S pombe; C006698:carbendazim