title: Efficient whole-cell biotransformation of 5-(hydroxymethyl)furfural into FDCA, 2,5-furandicarboxylic acid.
abstract: 2,5-furandicarboxylic acid (FDCA) is a promising bio-based platform chemical that may serve as a 'green' substitute for terephthalate in polyesters. Recently, a novel HMF/furfural oxidoreductase from Cupriavidus basilensis HMF14 was identified that converts 5-(hydroxymethyl)furfural (HMF) into FDCA. The hmfH gene encoding this oxidoreductase was introduced into Pseudomonas putida S12 and the resulting whole-cell biocatalyst was employed to produce FDCA from HMF. In fed-batch experiments using glycerol as the carbon source, 30.1 g l(-1) of FDCA was produced from HMF at a yield of 97%. FDCA was recovered from the culture broth as a 99.4% pure dry powder, at 76% recovery using acid precipitation and subsequent tetrahydrofuran extraction.
authors.affiliation: Bio-based Sustainable Industrial Chemistry (B-Basic), Delft, The Netherlands. f.w.koopman@tudelft.nl
authors.forename: Frank
authors.initials: F
authors.lastname: Koopman
authors.forename: Nick
authors.initials: N
authors.lastname: Wierckx
authors.forename: Johannes H
authors.initials: JH
authors.lastname: de Winde
authors.forename: Harald J
authors.initials: HJ
authors.lastname: Ruijssenaars
mesh_terms: D055162:Biocatalysis; D001711:Biotransformation; D003998:Dicarboxylic Acids / Q000378:metabolism*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D005663:Furans / Q000378:metabolism*; D016958:Pseudomonas putida / Q000378:metabolism*
chemical_list: D003998:Dicarboxylic Acids; D005663:Furans; C008046:5-hydroxymethylfurfural; C551400:2,5-furandicarboxylic acid; D005662:Furaldehyde