title: Endoglycosidase and glycoamidase release of N-linked glycans.
abstract: Nearly all proteins entering the lumen of the endoplasmic reticulum (ER) become glycosylated en route to a cellular organelle, the plasma membrane, or the extracellular space. Many glycans can be attached to proteins, but the most common are the N-linked glycans (oligosaccharides). These chains are added very soon after a protein enters the ER, but they undergo extensive remodeling (processing), especially in the Golgi. Processing changes the sensitivity of the N-glycan to enzymes that cleave entire sugar chains or individual monosaccharides, which also changes the migration of the protein on SDS gels. These changes can be used to indicate when a protein has passed a particular subcellular location. This unit details some of the methods used to track a protein as it trafficks from the ER to the Golgi toward its final location.
authors.affiliation: Sanford-Burnham Medical Research Institute, La Jolla, California.
authors.forename: Hudson H
authors.initials: HH
authors.lastname: Freeze
authors.affiliation: Sanford-Burnham Medical Research Institute, La Jolla, California.
authors.forename: Christian
authors.initials: C
authors.lastname: Kranz
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry; D000581:Amidohydrolases / Q000737:chemistry*; D002236:Carbohydrate Conformation; D004591:Electrophoresis, Polyacrylamide Gel; D024202:Electrophoretic Mobility Shift Assay; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006031:Glycosylation; D006056:Golgi Apparatus / Q000378:metabolism*; D047468:Immunoprecipitation; D008358:Mannose / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000737:chemistry; D021381:Protein Transport
chemical_list: D006023:Glycoproteins; D006025:Glycosaminoglycans; D009844:Oligosaccharides; D006026:Glycoside Hydrolases; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D008358:Mannose; D000117:Acetylglucosamine