title: Strobilanthes crispus attenuates renal carcinogen, iron nitrilotriacetate (Fe-NTA)-mediated oxidative damage of lipids and DNA.
abstract: This study was aimed to evaluate the effect of Strobilanthes crispus extract for possible protection against lipid peroxidation and DNA damage induced by iron nitrilotriacetate (Fe-NTA) and hydrogen peroxide (H(2)O(2)). Fe-NTA is a potent nephrotoxic agent and induces acute and subacute renal proximal tubular necrosis by catalyzing the decomposition of H(2)O(2)-derived production of hydroxyl radicals, which are known to cause lipid peroxidation and DNA damage. Incubation of postmitochondrial supernatant and/or calf thymus DNA with H(2)O(2) (40 mM) in the presence of Fe-NTA (0.1 mM) induces lipid peroxidation and DNA damage to about 2.3-fold and 2.9-fold, respectively, as compared to control (P < 0.05). In lipid peroxidation protection studies, S. crispus treatment showed a dose-dependent inhibition (45-53% inhibition, P < 0.05) of Fe-NTA and H(2)O(2) induced lipid peroxidation. Similarly, in DNA damage protection studies, S. crispus treatment also showed a dose-dependent inhibition (18-30% inhibition, P < 0.05) of DNA damage. In addition, the protection was closely related to the content of phenolic compounds as evident by S. crispus extract showing the value of 124.48 mg/g total phenolics expressed as gallic acid equivalent (GAE, mg/g of extract). From these studies, it is concluded that S. crispus inhibits peroxidation of membrane lipids and DNA damage induced by Fe-NTA and H(2)O(2) and possesses the potential to be used to treat or prevent degenerative diseases where oxidative stress is implicated.
authors.affiliation: Biotechnology Research Institute, University Malaysia Sabah, Locked Bag No 2073, 88999 Kota Kinabalu, Sabah, Malaysia. miqbal2k2008@hotmail.com
authors.forename: Mohammad
authors.initials: M
authors.lastname: Iqbal
authors.forename: Muhammad Dawood
authors.initials: MD
authors.lastname: Shah
authors.forename: Chau Ann
authors.initials: CA
authors.lastname: Lie
authors.forename: Chin Kim
authors.initials: CK
authors.lastname: San
mesh_terms: D002273:Carcinogens / Q000633:toxicity; D004249:DNA Damage / Q000187:drug effects*; D005290:Ferric Compounds / Q000633:toxicity; D006861:Hydrogen Peroxide; D007668:Kidney / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects*; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D002273:Carcinogens; D005290:Ferric Compounds; D010636:Phenols; D010936:Plant Extracts; D020011:Protective Agents; D006861:Hydrogen Peroxide; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate