Mesenchymal stem cell-encapsulated collagen microspheres for bone tissue engineering.
There is a demonstrated clinical need for alternatives of autologous fresh bone graft with excellent biological performance in osteoconductivity, osteoinductivity, and osteogenicity. We previously developed a collagen microencapsulation technology entrapping bone marrow-derived mesenchymal stem cells (MSCs) in a biomimetic collagen fiber meshwork and produced injectable collagen-MSC microspheres. In this study, we hypothesize that injectable microspheres with osteoconductivity, osteogenicity, and osteoinductivity can be fabricated by differentiating the encapsulated MSCs, from either human or mouse sources, toward osteogenic lineages in these three-dimensional microspheres. The osteogenicity, osteoconductivity, and osteoinductivity of the microspheres were evaluated in vitro. Osteogenic markers of the differentiating MSCs including alkaline phosphatase and calcium deposition showed positive staining. Osteoconductivity of the collagen meshwork in the microsphere was demonstrated by the presence of calcium phosphate deposits among the collagen fibers and by the significantly increased calcium content extracted from the microspheres. Moreover, osteoinductivity of the MSC-encapsulated microspheres was demonstrated by the ability to induce osteogenic differentiation of undifferentiated MSCs in both contact and noncontact coculture. This study contributes toward the future development of injectable alternatives for fresh bone grafts using autologous MSCs.
Chan
Barbara Pui
BP
Medical Engineering Program, Department of Mechanical Engineering, The University of Hong Kong, Hong Kong Special Administrative Region, China. bpchan@hkucc.hku.hk
Hui
Ting Yan
TY
Wong
Mei Yi
MY
Yip
Kevin Hak Kong
KH
Chan
Godfrey Chi Fung
GC
D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D001842:Bone and Bones / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D004339:Drug Compounding; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008863:Microspheres; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*
D003094:Collagen