title: Neuropathy in a rat model of mild diabetes induced by multiple low doses of streptozotocin: effects of the antioxidant stobadine in comparison with a high-dose alpha-lipoic acid treatment.
abstract: Hyperglycaemia-induced oxidative stress makes an important contribution to the aetiology of diabetic neuropathy. The aim of our study was to evaluate the effect of the antioxidant stobadine (STB) in comparison with a treatment by a high-dose of alpha-lipoic acid (ALA), on the neurological consequences of chronic hyperglycaemia in an animal model of diabetes in Wistar rats (16 weeks old), made diabetic by streptozotocin (STZ 3 x 20 mg i.v.). Neuropathy was evaluated electrophysiologically by measuring motor nerve conduction velocity (NCV) in the 4th and 8th week in vivo and motor NCV and resistance to ischaemic conduction failure (RICF) of the sciatic nerve in the 10th week of the experiment in vitro. The therapy with ALA (100 mg/kg i.p., 5 times a week) and STB (25 mg/kg i.p., 5 times a week) had a significant effect on NCV in vivo in the 8th week of the experiment and no effect in the 10th week in vitro. The RICF elevated in diabetic animals was significantly modified by ALA. The effect of the antioxidant STB on NCV was comparable with that of ALA, while RICF was affected only by ALA. We conclude that treatment with appropriate antioxidants might partially prevent nerve dysfunction in diabetic rats.
authors.affiliation: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia. silvia.skalska@yahoo.com
authors.forename: Silvia
authors.initials: S
authors.lastname: Skalská
authors.forename: Pavol
authors.initials: P
authors.lastname: Kucera
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Goldenberg
authors.forename: Milan
authors.initials: M
authors.lastname: Stefek
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kyselová
authors.forename: Pavol
authors.initials: P
authors.lastname: Jariabka
authors.forename: Alena
authors.initials: A
authors.lastname: Gajdosíková
authors.forename: Katarína
authors.initials: K
authors.lastname: Klobucníková
authors.forename: Pavel
authors.initials: P
authors.lastname: Traubner
authors.forename: Svorad
authors.initials: S
authors.lastname: Stolc
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D002243:Carbolines / Q000008:administration & dosage*; D003929:Diabetic Neuropathies / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology*; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D009431:Neural Conduction / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D008063:Thioctic Acid / Q000008:administration & dosage; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D002243:Carbolines; D013311:Streptozocin; D008063:Thioctic Acid; C100106:dicarbine