Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses.
Members of the family of 70-kD heat shock proteins (HSP70 s) play various stress-protective roles in plants. In this study, a wheat HSP70 gene was isolated from a suppression subtractive hybridization (SSH) cDNA library of wheat leaves infected by Puccinia striiformis f. sp. tritici. The gene, that was designated as TaHSC70, was predicted to encode a protein of 690 amino acids, with a molecular mass of 73.54 KDa and a pI of 5.01. Further analysis revealed the presence of a conserved signature that is characteristic for HSP70s and phylogenetic analysis demonstrated that TaHSC70 is a homolog of chloroplast HSP70s. TaHSC70 mRNA was present in leaves of both green and etiolated wheat seedlings and in stems and roots. The transcript level in roots was approximately threefold less than in leaves but light-dark treatment did not charge TaHSC70 expression. Following heat shock of wheat seedlings at 40°C, TaHSC70 expression increased in leaves of etiolated seedlings but remained stable at the same level in green seedlings. In addition, TaHSC70 was differentially expressed during an incompatible and compatible interaction with wheat-stripe rust, and there was a transient increase in expression upon treatment with methyl jasmonate (MeJA) treatment. Salicylic acid (SA), ethylene (ET) and abscisic acid (ABA) treatments had no influence on TaHSC70 expression. These results suggest that TaHSC70 plays a role in stress-related responses, and in defense responses elicited by infection with stripe rust fungus and does so via a JA-dependent signal transduction pathway.
Duan
Ying-Hui
YH
College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, 712100, Shaanxi, People's Republic of China.
Guo
Jun
J
Ding
Ke
K
Wang
Shu-Juan
SJ
Zhang
Hong
H
Dai
Xi-Wei
XW
Chen
Yue-Ying
YY
Govers
Francine
F
Huang
Li-Li
LL
Kang
Zhen-Sheng
ZS
D001487:Basidiomycota / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D017343:Genes, Plant; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D008027:Light; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000382:microbiology / Q000528:radiation effects; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D014908:Triticum / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology / Q000528:radiation effects
D018076:DNA, Complementary; D018840:HSP70 Heat-Shock Proteins; D010937:Plant Growth Regulators