title: Molecular characterization of beta-lactamase genes and their genetic structures in Acinetobacter genospecies 3 isolates in Taiwan.
abstract: The genetic structure of beta-lactamases in Acinetobacter genospecies 3 (AG3) isolates in Taiwan was studied to analyze their high rates of resistance to beta-lactams, including carbapenems (57.9%). bla(IMP-1) and bla(IMP-8) were located in a class 1 integron. bla(OXA-58) was bracketed by ISAba3. A novel TnpF-like integrase gene was identified upstream of bla(VEB-3). Adjacent to the 5' sequence of the bla(ADC) gene, folE was identified. Four new Acinetobacter-derived cephalosporinase (ADC) enzymes were found, which clustered phylogenetically with published AG3 ADC proteins.
authors.affiliation: Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Li-Yueh
authors.initials: LY
authors.lastname: Huang
authors.forename: Po-Liang
authors.initials: PL
authors.lastname: Lu
authors.forename: Te-Li
authors.initials: TL
authors.lastname: Chen
authors.forename: Fang-Yee
authors.initials: FY
authors.lastname: Chang
authors.forename: Chang-Phone
authors.initials: CP
authors.lastname: Fung
authors.forename: L K
authors.initials: LK
authors.lastname: Siu
mesh_terms: D000150:Acinetobacter / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D002510:Cephalosporinase / Q000235:genetics; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D006801:Humans; D066298:In Vitro Techniques; D032023:Integrons / Q000235:genetics; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D013624:Taiwan / Q000453:epidemiology; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D002510:Cephalosporinase; D001618:beta-Lactamases