Establishment of a novel ghrelin-producing cell line.
To establish a tool to study ghrelin production and secretion in vitro, we developed a novel ghrelin-producing cell line, MGN3-1 (mouse ghrelinoma 3-1) cells from a gastric ghrelin-producing cell tumor derived from ghrelin-promoter Simian virus 40-T-antigen transgenic mice. MGN3-1 cells preserve three essential characteristics required for the in vitro tool for ghrelin research. First, MGN3-1 cells produce a substantial amount of ghrelin at levels approximately 5000 times higher than that observed in TT cells. Second, MGN3-1 cell expressed two key enzymes for acyl modification and maturation of ghrelin, namely ghrelin O-acyltransferase for acylation and prohormone convertase 1/3 for maturation and the physiological acyl modification and maturation of ghrelin were confirmed. Third, MGN3-1 cells retain physiological regulation of ghrelin secretion, at least in regard to the suppression by somatostatin and insulin, which is well established in in vivo studies. Thus, MGN3-1 cells are the first cell line derived from a gastric ghrelin-producing cell preserving secretion of substantial amounts of ghrelin under physiological regulation. This cell line will be a useful tool for both studying the production and secretion of ghrelin and screening of ghrelin-modulating drugs.
Iwakura
Hiroshi
H
54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan. hiwaku@kuhp.kyoto-u.ac.jp
Li
Yushu
Y
Ariyasu
Hiroyuki
H
Hosoda
Hiroshi
H
Kanamoto
Naotetsu
N
Bando
Mika
M
Yamada
Go
G
Hosoda
Kiminori
K
Nakao
Kazuwa
K
Kangawa
Kenji
K
Akamizu
Takashi
T
D000818:Animals; D015153:Blotting, Western; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D054439:Ghrelin / Q000097:blood* / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D046529:Microscopy, Electron, Transmission; D020133:Reverse Transcriptase Polymerase Chain Reaction
D054439:Ghrelin