title: Effects of adipose tissue-derived stem cell therapy after myocardial infarction: impact of the route of administration.
abstract: BACKGROUND
  Cell-based therapies offer a promising approach to reducing the short-term mortality rate associated with heart failure after a myocardial infarction. The aim of the study was to analyze histological and functional effects of adipose tissue-derived stem cells (ADSCs) after myocardial infarction and compare 2 types of administration pathways.
  
  
  RESULTS
  ADSCs from 28 pigs were labeled by transfection. Animals that survived myocardial infarction (n = 19) received: intracoronary culture media (n = 4); intracoronary ADSCs (n = 5); transendocardial culture media (n = 4); or transendocardial ADSCs (n = 6). At 3 weeks' follow-up, intracoronary and transendocardial administration of ADSCs resulted in similar rates of engrafted cells (0.85 [0.19-1.97] versus 2 [1-2] labeled cells/cm(2), respectively; P = NS) and some of those cells expressed smooth muscle cell markers. The intracoronary administration of ADSCs was more effective in increasing the number of small vessels than transendocardial administration (223 +/- 40 versus 168 +/- 35 vessels/mm(2); P < .05). Ejection fraction was not modified by stem cell therapy.
  
  
  CONCLUSIONS
  This is the first study to compare intracoronary and transendocardial administration of autologous ADSCs in a porcine model of myocardial infarction. Both pathways of ADSCs delivery are feasible, producing a similar number of engrafted and differentiated cells, although intracoronary administration was more effective in increasing neovascularization.
authors.affiliation: Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain. mrigol@clinic.ub.es
authors.forename: Montserrat
authors.initials: M
authors.lastname: Rigol
authors.forename: Núria
authors.initials: N
authors.lastname: Solanes
authors.forename: Jordi
authors.initials: J
authors.lastname: Farré
authors.forename: Santiago
authors.initials: S
authors.lastname: Roura
authors.forename: Mercè
authors.initials: M
authors.lastname: Roqué
authors.forename: Antonio
authors.initials: A
authors.lastname: Berruezo
authors.forename: Neus
authors.initials: N
authors.lastname: Bellera
authors.forename: Laura
authors.initials: L
authors.lastname: Novensà
authors.forename: David
authors.initials: D
authors.lastname: Tamborero
authors.forename: Cristina
authors.initials: C
authors.lastname: Prat-Vidal
authors.forename: M A Angeles
authors.initials: MA
authors.lastname: Huzman
authors.forename: Montserrat
authors.initials: M
authors.lastname: Batlle
authors.forename: Margo
authors.initials: M
authors.lastname: Hoefsloot
authors.forename: Marta
authors.initials: M
authors.lastname: Sitges
authors.forename: José
authors.initials: J
authors.lastname: Ramírez
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Dantas
authors.forename: Anna
authors.initials: A
authors.lastname: Merino
authors.forename: Ginés
authors.initials: G
authors.lastname: Sanz
authors.forename: Josep
authors.initials: J
authors.lastname: Brugada
authors.forename: Antoni
authors.initials: A
authors.lastname: Bayés-Genís
authors.forename: Magda
authors.initials: M
authors.lastname: Heras
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D002478:Cells, Cultured; D004699:Endocardium / Q000473:pathology / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D009203:Myocardial Infarction / Q000473:pathology / Q000601:surgery*; D033581:Stem Cell Transplantation / Q000379:methods*; D013552:Swine; D013997:Time Factors