Application of a modified enzyme-linked immunosorbent assay for 3-amino-2-oxazolidinone residue in aquatic animals.
Furazolidone has been banned from use in food animals because of its carcinogenicity and mutagenicity, but its continued misuse is widespread in aquacultures. Therefore, there is an urgent need for a simple, reliable, and rapid method for the detection of its marker residue, 3-amino-2-oxazolidinone (AOZ), in aquatic products. In this regard, we modified a simplified indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) to address this need. A good linearity was achieved over a concentration range of 0.05-12.15 microg L(-1), and the IC(50) value was 0.96 microg L(-1). The sample preparation was simple and effective included water bath treatments, acid hydrolysis combined with overnight derivatization of AOZ by benzaldehyde. The limit of detection and the limit of quantification were 0.15 and 0.3 microg kg(-1). The recoveries of AOZ in all tissues were between 78.0-95.3% at the levels of 0.3, 1.0, and 2.0 microg kg(-1). The inter-assay variability was less than 19.1%. The modified ic-ELISA was applied in quantification of AOZ elimination in carp. The results showed that AOZ was quite difficult to eliminate. Good correlations of the results obtained by ELISA and LC-MS/MS were observed in incurred carp muscle (r=0.9923) and carp plasma (r=0.9915) at the levels of 2.5-571.8 microg kg(-1) (microg L(-1)). Better results were obtained by modified ic-ELISA when compared with commercial ELISA kit. Therefore, the present assay is considered a rapid, accurate, reliable, and inexpensive method for the detection of furazolidone-residues in the edible tissues of aquatic animals.
Liu
Yu
Y
National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, PR China.
Peng
Dapeng
D
Huang
Lingli
L
Wang
Yulian
Y
Chang
Chao
C
Ihsan
Awais
A
Tao
Yanfei
Y
Yang
Bo
B
Yuan
Zonghui
Z
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000378:metabolism; D002347:Carps / Q000378:metabolism; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005664:Furazolidone / Q000378:metabolism; D023303:Oxazolidinones / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry
D000891:Anti-Infective Agents, Local; D023303:Oxazolidinones; D005664:Furazolidone; C000209:3-amino-2-oxazolidinone