title: Mass spectrometric investigations on lactate adduction to equine myoglobin.
abstract: Research focused on determining the fundamental mechanisms by which lactate influences color stability has not considered a direct effect of lactate on myoglobin. Thus, the objective of this study was to use Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry to examine lactate adduction to myoglobin. Equine oxymyoglobin and equine carboxymyoglobin (0.15mM) were incubated with sodium lactate (200mM) at 4 degrees C, pH 5.6 in 50mM sodium citrate buffer or at 37 degrees C, pH 7.4 in 50mM sodium phosphate buffer, simulating typical meat storage and physiological conditions, respectively. Controls consisted of myoglobin plus a volume of deionized water equivalent to that used to deliver the lactate treatments. No peaks corresponding to lactate-Mb adducts could be detected in the mass spectra of samples incubated up to 360min at pH 7.4, 37 degrees C or 8days at pH 5.6 and 4 degrees C. Our results suggest that lactate did not form covalent adducts with equine oxy- and carboxy-myoglobin.
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Mancini
authors.forename: S P
authors.initials: SP
authors.lastname: Suman
authors.forename: M K R
authors.initials: MK
authors.lastname: Konda
authors.forename: R
authors.initials: R
authors.lastname: Ramanathan
authors.forename: P
authors.initials: P
authors.lastname: Joseph
authors.forename: C M
authors.initials: CM
authors.lastname: Beach
mesh_terms: D000818:Animals; D003116:Color; D006736:Horses; D007773:Lactates / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry*; D010084:Oxidation-Reduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D007773:Lactates; D009211:Myoglobin; C018013:carboxymyoglobin; C017595:oxymyoglobin