Spectroscopic study of the reaction mechanism of buspirone interaction with iodine and tetracyanoethylene reagents and its applications.
The reactions between the drug buspirone (busp) in its base form and iodine amphoteric reagent (n-donor and/or sigma-acceptor) and with tetracyanoethylene as a pi-acceptor reagent (TCNE) have been studied spectrophotometrically at different reactant concentrations, time intervals, temperatures, and with different solvents and wavelengths, with the aim of selecting the conditions that give the most suitable molar extinction coefficients. This study aims chiefly to throw light on the nature of these reactions and to select the most proper conditions for spectrophotometric application of these reagents to determine this biologically active drug used in treating different diseases. The reaction mechanism involves the formation of busp-I(2) outer and inner sphere complexes. The separated busp-I(2) solid product obtained was investigated using elemental analyses, FT-IR, thermal analyses (TA) and electron ionization mass spectrometry (EI-MS) and was found to be biologically active. The reaction mechanism of busp-TCNE involves the formation of a charge transfer (CT) complex. The analytical parameters of the proposed spectrophotometric procedures were calculated. These procedures were applied in the analysis of busp in its formulations as a drug used to treat psychiatric illnesses. The values of the Sandell sensitivity, standard deviation (SD), relative standard deviation (RSD) and recovery percentage show the high sensitivity of these procedures. This study also presents a promising new busp-I(2) drug derivative that can be used more efficiently for the same purposes as its parent. It gives a clear idea about the possible metabolites and metabolic pathways of busp and its derivative that may occur in vivo.
Zayed
M A
MA
Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt. mazayed429@yahoo.com
El-Habeeb
Abeer A
AA
D002065:Buspirone / Q000032:analysis* / Q000737:chemistry; D005030:Ethylenes / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D007455:Iodine / Q000737:chemistry; D009570:Nitriles / Q000737:chemistry; D017366:Serotonin Receptor Agonists / Q000032:analysis* / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D005030:Ethylenes; D007202:Indicators and Reagents; D009570:Nitriles; D017366:Serotonin Receptor Agonists; D012997:Solvents; D007455:Iodine; C003393:tetracyanoethylene; D002065:Buspirone