Reduced expression of SK3 and IK1 channel proteins in the cavernous tissue of diabetic rats.
The small (SK3) and intermediate (IK1) conductance calcium-activated potassium channels could have key roles in the endothelium-dependent hyperpolarization factor pathway, which is believed to contribute to normal penile erection function. We aimed to investigate the expression of SK3 and IK1 in diabetic rodents. The experimental diabetes model was induced in 8-week-old male Sprague-Dawley rats (250-300 g) by a single administration of streptozotocin. Both the diabetes mellitus group (DM group, n = 20) and the control group (NDM group, n = 10) were injected with a low dose of apomorphine to allow for the measurement and comparison of the corresponding penile erections. The mRNA and protein expression levels of SK3 and IK1 were measured by reverse transcription polymerase chain reaction and western blot, respectively. Erectile function was significantly decreased in the DM group compared with control group (P < 0.05). The mRNA and protein expression levels of SK3 and IK1 were reduced in the cavernous tissue of diabetic rats compared with the control group (P < 0.05). Diabetes inhibits mRNA and protein expression of both SK3 and IK1 in the cavernous tissue of diabetic rats. This could play a key role in the development of erectile dysfunction in diabetic rats.
Zhu
Jin-Hai
JH
Department of Urology, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, China.
Jia
Rui-Peng
RP
Xu
Lu-Wei
LW
Wu
Jian-Ping
JP
Wang
Zi-Zheng
ZZ
Wang
Shu-Kui
SK
Bo
Cheng-Jia
CJ
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000503:physiopathology; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D010410:Penile Erection; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D051640:Small-Conductance Calcium-Activated Potassium Channels / Q000096:biosynthesis* / Q000235:genetics
D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; C496901:Kcnn3 protein, rat; D012333:RNA, Messenger; D051640:Small-Conductance Calcium-Activated Potassium Channels