[Effect of magnesium-free on glucocorticoid receptor expression in primary cultured cortical neurons of fetal rats in vitro].
OBJECTIVE
To study the changes of glucocorticoid receptor (GR) expression in embryonic rat cortical neurons exposed to transient Mg(2+)-free treatment.


METHODS
Six days after rat cortical neuronal cultures, two groups were created based on the medium to which were transiently exposed. The control group was exposed to a physiological solution (PS), and the Mg(2+)-free group was exposed to the same medium as the control group except for the removal of magnesium. The expression of GR mRNA and protein was determined by real-time PCR and immunocytochemistry staining 1, 7 and 12 days after transient Mg(2+)-free treatment.


RESULTS
Compared to the control group, the Mg(2+)-free group displayed the significantly less accumulated optical density (AOD) of GR immunoreactivity 12 days after transient Mg(2+)-free treatment (p<0.05). On the contrary, GR mRNA expression increased significantly 1 and 7 days after transient Mg(2+)-free treatment in the Mg(2+)-free group (p<0.05).


CONCLUSIONS
GR expression is modified following Mg-free-induced injury in cultured developing neurons in rats.
Bo
Tao
T
Department of Pediatrics, Second Xiangya Hospital, Central South University, Changsha 410011, China. boily@yeah.net
Yi
Lu
L
Wang
Tuan-Mei
TM
Li
Jian
J
Li
Xing-Fang
XF
Mao
Ding-An
DA
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D005333:Fetus / Q000378:metabolism*; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology; D008274:Magnesium / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D010913:Pituitary-Adrenal System / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000032:analysis / Q000235:genetics*
D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D008274:Magnesium