title: Phytochemical characteristics and hypoglycaemic activity of fraction from mushroom Inonotus obliquus.
abstract: BACKGROUND
  Inonotus obliquus is a medicinal mushroom that has been used as an effective agent to treat various diseases such as diabetes, tuberculosis and cancer. In order to elucidate the active fraction and its constituents, the effects of ethyl acetate fraction from I. obliquus (EAFI) on hyperglycaemia were investigated and the main constituents of EAFI were isolated and identified.
  
  
  RESULTS
  EAFI treatment led to a significant decrease in blood glucose level (P < 0.05) in alloxan-induced diabetic mice. It significantly decreased the total cholesterol level in serum, increased glutathione peroxidase activity and improved the growth physiological characteristics. In addition, EAFI treatment decreased the levels of triglyceride and malondialdehyde and increased the high-density lipoprotein cholesterol level in serum and the hepatic glycogen level in liver of diabetic mice. Five compounds were isolated from EAFI and identified as lanosterol (1), 3beta-hydroxy-lanosta-8,24-diene-21-al (2), inotodiol (3), ergosterol peroxide (4) and trametenolic acid (5) by spectral methods. Inotodiol and trametenolic acid were found to have an inhibitory effect on alpha-amylase activity and a scavenging effect on 1,1-diphenyl-2-picrylhydrazyl radicals.
  
  
  CONCLUSIONS
  EAFI showed significant antihyperglycaemic and antilipidperoxidative effects in alloxan-induced diabetic mice. Terpenoid and sterol compounds appeared to be the major active constituents of I. obliquus.
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Xueming
authors.initials: X
authors.lastname: Lu
authors.forename: Haixia
authors.initials: H
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Dong
authors.forename: Lingling
authors.initials: L
authors.lastname: Fu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D001786:Blood Glucose / Q000378:metabolism*; D008076:Cholesterol, HDL / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D005979:Glutathione Peroxidase / Q000097:blood; D006003:Glycogen / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008815:Mice, Inbred Strains; D014280:Triglycerides / Q000097:blood; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D000975:Antioxidants; D001688:Biological Products; D001786:Blood Glucose; D008076:Cholesterol, HDL; D007004:Hypoglycemic Agents; D014280:Triglycerides; D008315:Malondialdehyde; D006003:Glycogen; D005979:Glutathione Peroxidase; D000516:alpha-Amylases