Seven direct methods for measuring HDL and LDL cholesterol compared with ultracentrifugation reference measurement procedures.
BACKGROUND
Methods from 7 manufacturers and 1 distributor for directly measuring HDL cholesterol (C) and LDL-C were evaluated for imprecision, trueness, total error, and specificity in nonfrozen serum samples.


METHODS
We performed each direct method according to the manufacturer's instructions, using a Roche/Hitachi 917 analyzer, and compared the results with those obtained with reference measurement procedures for HDL-C and LDL-C. Imprecision was estimated for 35 runs performed with frozen pooled serum specimens and triplicate measurements on each individual sample. Sera from 37 individuals without disease and 138 with disease (primarily dyslipidemic and cardiovascular) were measured by each method. Trueness and total error were evaluated from the difference between the direct methods and reference measurement procedures. Specificity was evaluated from the dispersion in differences observed.


RESULTS
Imprecision data based on 4 frozen serum pools showed total CVs <3.7% for HDL-C and <4.4% for LDL-C. Bias for the nondiseased group ranged from -5.4% to 4.8% for HDL-C and from -6.8% to 1.1% for LDL-C, and for the diseased group from -8.6% to 8.8% for HDL-C and from -11.8% to 4.1% for LDL-C. Total error for the nondiseased group ranged from -13.4% to 13.6% for HDL-C and from -13.3% to 13.5% for LDL-C, and for the diseased group from -19.8% to 36.3% for HDL-C and from -26.6% to 31.9% for LDL-C.


CONCLUSIONS
Six of 8 HDL-C and 5 of 8 LDL-C direct methods met the National Cholesterol Education Program total error goals for nondiseased individuals. All the methods failed to meet these goals for diseased individuals, however, because of lack of specificity toward abnormal lipoproteins.
Miller
W Greg
WG
Virginia Commonwealth University, Richmond, VA, USA. gmiller@vcu.edu
Myers
Gary L
GL
Sakurabayashi
Ikunosuke
I
Bachmann
Lorin M
LM
Caudill
Samuel P
SP
Dziekonski
Andrzej
A
Edwards
Selvin
S
Kimberly
Mary M
MM
Korzun
William J
WJ
Leary
Elizabeth T
ET
Nakajima
Katsuyuki
K
Nakamura
Masakazu
M
Nilsson
Göran
G
Shamburek
Robert D
RD
Vetrovec
George W
GW
Warnick
G Russell
GR
Remaley
Alan T
AT
D001774:Blood Chemical Analysis / Q000379:methods*; D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood*; D006801:Humans; D012015:Reference Standards; D012680:Sensitivity and Specificity; D014461:Ultracentrifugation
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL