Neuroglycan C, a brain-specific chondroitin sulfate proteoglycan, interacts with pleiotrophin, a heparin-binding growth factor.
Neuroglycan C (NGC) is a transmembrane-type chondroitin sulfate proteoglycan that promotes neurite outgrowth. To identify the ligand of NGC, we applied a detergent-solubilized membrane fraction of fetal rat brains to an NGC-immobilized affinity column. Several proteins were eluted from the column including an 18 kDa-band protein recognized by an anti-pleiotrophin antibody. The binding of pleiotrophin (PTN) to NGC was confirmed by a quartz crystal microbalance method and had a Kd of 8.7 nM. PTN bound to the acidic amino acid cluster of the NGC extracellular domain. In addition, PTN bound to both chondroitin sulfate-bearing NGC and chondroitinase-treated NGC prepared from the neonatal rat brain. These results suggest that NGC interacts with PTN.
Nakanishi
Keiko
K
Department of Perinatology, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi, Japan. nakanishi@inst-hsc.jp
Tokita
Yoshihito
Y
Aono
Sachiko
S
Ida
Michiru
M
Matsui
Fumiko
F
Higashi
Yujiro
Y
Oohira
Atsuhiko
A
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D019765:Chondroitin ABC Lyase / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism*; D008024:Ligands; D008565:Membrane Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats
D002352:Carrier Proteins; C485738:Cspg5 protein, rat; D016207:Cytokines; D008024:Ligands; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011509:Proteoglycans; C066845:pleiotrophin; D019765:Chondroitin ABC Lyase