title: Identification and characterization of Th cell epitopes in MrkD adhesin of Klebsiella pneumoniae.
abstract: In this study, we identified the Th epitopes in MrkD of Klebsiella pneumoniae, an excellent vaccine candidate antigen. By using the RANKPEP prediction algorithm, we have identified and characterized three Th epitopes within the MrkD antigen, which can be recognized by CD4+ T cells from BALB/c (H-2(d)) mice. They were M(221-235), M(175-189), and M(264-278). These epitopes have important value for studying the immune response of K. pneumoniae infection and for designing effective vaccine against K. pneumoniae.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Li
authors.forename: Wen-yu
authors.initials: WY
authors.lastname: Han
authors.forename: Lian-cheng
authors.initials: LC
authors.lastname: Lei
authors.forename: Chang-jiang
authors.initials: CJ
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.forename: Chong-tao
authors.initials: CT
authors.lastname: Du
authors.forename: Tao-feng
authors.initials: TF
authors.lastname: Du
authors.forename: Jing-min
authors.initials: JM
authors.lastname: Gu
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D018604:Epitope Mapping; D005260:Female; D036781:Fimbriae Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007710:Klebsiella Infections / Q000276:immunology* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; C058768:Klebsiella pneumoniae type 3 fimbrial adhesin; D036781:Fimbriae Proteins