title: Counter-current chromatographic separation of nucleic acid constituents with a hydrophilic solvent system.
abstract: Nucleic acid constituents such as nucleobases, nucleosides and nucleotides were separated by counter-current chromatography using type J coil planet centrifuge. The separation was performed with a hydrophilic solvent system composed of 1-propanol/800 mM potassium phosphate buffer (pH 7.4) (1:1, v/v) by eluting the lower aqueous phase at a flow-rate of 0.5 ml/min. Eight selected nucleic acid constituents (4.0 mg, 0.5 mg of each), uridine monophosphate (UMP), adenosine monophosphate (AMP), deoxyadenosine monophosphate (dAMP), uridine, urasile, deoxy uridine, adenosine and adenine were well resolved within 160 min.
authors.affiliation: Division of Pharmaceutical and Biomedical Analysis, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. sibusawa@toyaku.ac.jp
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Shibusawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shoji
authors.forename: Chihiro
authors.initials: C
authors.lastname: Suzuka
authors.forename: Akio
authors.initials: A
authors.lastname: Yanagida
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D009696:Nucleic Acids / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry*
chemical_list: D009696:Nucleic Acids; D012997:Solvents