title: Gene expression profile in rat hippocampus with and without memory deficit.
abstract: The cholinergic neuronal system, through its projections to the hippocampus, plays an important role in learning and memory. The aim of the study was to identify genes and networks in rat hippocampus with and without memory deficit. Genome-scale screening was used to analyze gene expression changes in rats submitted or not to intraparenchymal injection of 192 IgG-saporin and trained in spatial/object novelty tasks. Results showed learning processes were associated with significant expression of genes that could be grouped into several clusters of similar expression profiles and that are involved in biological functions, namely lipid metabolism, signal transduction, protein metabolism and modification, and transcription regulation. Memory loss following hippocampal cholinergic deafferentation was associated with significant expression of genes that did not show similar cluster organization. Only one cluster of genes could be identified; it included genes that would be involved in tissue remodeling. More important, most of the genes significantly altered in lesioned rats were down-regulated.
authors.affiliation: Université d'Aix-Marseille I, Laboratoire de Neurobiologie Intégrative et Adaptative, UMR/CNRS 6149, 3 Place Victor Hugo, 13331 Marseille Cedex 03, France. veronique.paban@univ-provence.fr <veronique.paban@univ-provence.fr>
authors.forename: Véronique
authors.initials: V
authors.lastname: Paban
authors.forename: Fernand
authors.initials: F
authors.lastname: Farioli
authors.forename: Béatrice
authors.initials: B
authors.lastname: Romier
authors.forename: Caroline
authors.initials: C
authors.lastname: Chambon
authors.forename: Béatrice
authors.initials: B
authors.lastname: Alescio-Lautier
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000911:Antibodies, Monoclonal; D005106:Exploratory Behavior / Q000502:physiology; D015870:Gene Expression; D020869:Gene Expression Profiling; D006624:Hippocampus / Q000378:metabolism*; D007858:Learning / Q000502:physiology; D008297:Male; D008569:Memory Disorders / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism*; D009483:Neuropsychological Tests; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar; D021641:Recognition, Psychology / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D013028:Space Perception / Q000502:physiology; D013997:Time Factors
chemical_list: C085696:192 IgG-saporin; D000911:Antibodies, Monoclonal; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins