Visualisation of lymphoid organ development.
This chapter provides information on imaging tools that can be employed to visualise and study lymphoid organ development. We focus on the use of genetically modified mouse models that take advantage of fluorescent protein expression in discrete cell populations, thus allowing live cell imaging during lymphoid organogenesis. We discuss approaches that allow characterisation of the cell types involved in the formation of lymphoid organs, including (i) functional assays in explant organ cultures and (ii) high-resolution whole-mount immunostaining methods, which are useful for the characterisation of specific cell populations in the context of the whole developing organ.
Veiga-Fernandes
Henrique
H
Division of Molecular Immunology, MRC National Institute for Medical Research, Mill Hill, London, UK.
Foster
Katie
K
Patel
Amisha
A
Coles
Mark
M
Kioussis
Dimitris
D
D000818:Animals; D019070:Cell Lineage; D002470:Cell Survival; D004210:Dissection; D004622:Embryo, Mammalian / Q000196:embryology; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008164:Luminescent Proteins / Q000378:metabolism; D008208:Lymphatic System / Q000166:cytology / Q000196:embryology*; D051379:Mice; D023421:Models, Animal; D038081:Organogenesis; D013194:Staining and Labeling
D008164:Luminescent Proteins