title: Simultaneous speciation of selenomethionine and 2-hydroxy-4-methylselenobutanoic acid by HPLC-ICP MS in biological samples.
abstract: An analytical method was developed for the simultaneous speciation of selenomethionine (SeMet) and 2-hydroxy-4-methylselenobutanoic acid (NutraSelen), a new SeMet precursor. The compounds could be baseline resolved by ion-pairing reversed-phase HPLC using ICP MS detection. Detection limits of 1 ng mL(-1) (Se content) could be reached. SELM-1 reference material was used to validate the SeMet measurement. Additionally, the quantification of NutraSelen was validated by standard addition together with checking the Se mass balance. The procedure developed was then applied to the monitoring of the conversion of NutraSelen into SeMet by yeast.
authors.affiliation: UT2A, Hélioparc Pau-Pyrénées, 2 avenue Pierre Angot, 64000 Pau, France. veronique.vacchina@univ-pau.fr
authors.forename: Véronique
authors.initials: V
authors.lastname: Vacchina
authors.forename: Marc
authors.initials: M
authors.lastname: Moutet
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Yadan
authors.forename: Frédéric
authors.initials: F
authors.lastname: de Baene
authors.forename: Bernard
authors.initials: B
authors.lastname: Kudla
authors.forename: Ryszard
authors.initials: R
authors.lastname: Lobinski
mesh_terms: D002087:Butyrates / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism; D018036:Selenium Compounds; D012645:Selenomethionine / Q000032:analysis* / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: C551033:2-hydroxy-4-methylselenobutanoic acid; D002087:Butyrates; D018036:Selenium Compounds; D012645:Selenomethionine