Determination of beta-agonists in pig feed, pig urine and pig liver using capillary electrophoresis with electrochemical detection.
The fast separation capability of a capillary electrophoresis with electrochemical detection (CE-ED) system was demonstrated by determining five beta-agonists including clenbuterol, metoprolol, ractopamine, isoxsuprine and salbutamol in several real samples. Factors influencing the separation and detection were examined and optimized. Under the optimum conditions, the five beta-agonists could be well separated within 15min at a separation voltage of 14kV in a 100mmoL H(3)BO(3)-Na(2)B(4)O(7) running buffer (pH 9.0). This method was successfully used in the analysis of clenbuterol, metoprolol, ractopamine, isoxsuprine and salbutamol in pig feed, pig urine and pig liver, providing a useful method for monitoring the illegal use of beta-agonists from growth promotion in food-producing animals.
Wang
Weiyu
W
Department of Chemistry, East China Normal University, North Zhongshan Rd., Shanghai 200062, PR China.
Zhang
Yulian
Y
Wang
Jinyan
J
Shi
Xue
X
Ye
Jiannong
J
D000318:Adrenergic beta-Agonists / Q000737:chemistry* / Q000652:urine*; D000818:Animals; D002021:Buffers; D004350:Drug Residues / Q000032:analysis*; D055664:Electrochemical Techniques / Q000662:veterinary*; D019075:Electrophoresis, Capillary / Q000379:methods / Q000662:veterinary*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine
D000318:Adrenergic beta-Agonists; D002021:Buffers