title: Baculovirus infection influences host protein expression in two established insect cell lines.
abstract: We identified host proteins that changed in response to host cell susceptibility to baculovirus infection. We used three baculovirus-host cell systems utilizing two cell lines derived from pupal ovaries, Hz-AM1 (from Helicoverpa zea) and Hv-AM1 (from Heliothis virescens). Hv-AM1 cells are permissive to Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and semi-permissive to H. zea single nucleopolyhedrovirus (HzSNPV). Hz-AM1 cells are non-permissive to AcMNPV. We challenged each cell line with baculovirus infection and after 24h determined protein identities by MALDI TOF/TOF mass spectrometry. For Hv-AM1 cells, 21 proteins were identified, and for Hz-AM1 cells, 19 proteins were newly identified (with 8 others having been previously identified). In the permissive relationship, 18 of the proteins changed in expression by 70% or more in AcMNPV infected Hv-AM1 cells as compared with non-infected controls; 12 were significantly decreased and 6 cellular proteins were significantly increased. We also identified 3 virus-specific proteins. In the semi-permissive infections, eight proteins decreased by 2-fold or more. Non-permissive interactions did not lead to substantial changes in host cell protein expression. We hypothesize that some of these proteins act in determining host cell specificity for baculoviruses.
authors.affiliation: USDA - Agricultural Research Service, Biological Control of Insects Research Laboratory, Columbia, MO 65203, USA. Holly.Popham@ars.usda.gov
authors.forename: Holly J R
authors.initials: HJ
authors.lastname: Popham
authors.forename: James J
authors.initials: JJ
authors.lastname: Grasela
authors.forename: Cynthia L
authors.initials: CL
authors.lastname: Goodman
authors.forename: Arthur H
authors.initials: AH
authors.lastname: McIntosh
mesh_terms: D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva / Q000821:virology; D009036:Moths / Q000821:virology*; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D019476:Insect Proteins