Single-step affinity purification for fungal proteomics.
A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches, which should be widely applicable, are included.
Liu
Hui-Lin
HL
Department of Molecular Genetics, Ohio State University, 496 W. 12th Ave., Columbus, OH 43210, USA.
Osmani
Aysha H
AH
Ukil
Leena
L
Son
Sunghun
S
Markossian
Sarine
S
Shen
Kuo-Fang
KF
Govindaraghavan
Meera
M
Varadaraj
Archana
A
Hashmi
Shahr B
SB
De Souza
Colin P
CP
Osmani
Stephen A
SA
D001233:Aspergillus nidulans / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005656:Fungal Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D005656:Fungal Proteins