title: Discovery of Phytophthora infestans genes expressed in planta through mining of cDNA libraries.
abstract: BACKGROUND
  Phytophthora infestans (Mont.) de Bary causes late blight of potato and tomato, and has a broad host range within the Solanaceae family. Most studies of the Phytophthora--Solanum pathosystem have focused on gene expression in the host and have not analyzed pathogen gene expression in planta.
  
  
  RESULTS
  We describe in detail an in silico approach to mine ESTs from inoculated host plants deposited in a database in order to identify particular pathogen sequences associated with disease. We identified candidate effector genes through mining of 22,795 ESTs corresponding to P. infestans cDNA libraries in compatible and incompatible interactions with hosts from the Solanaceae family.
  
  
  CONCLUSIONS
  We annotated genes of P. infestans expressed in planta associated with late blight using different approaches and assigned putative functions to 373 out of the 501 sequences found in the P. infestans genome draft, including putative secreted proteins, domains associated with pathogenicity and poorly characterized proteins ideal for further experimental studies. Our study provides a methodology for analyzing cDNA libraries and provides an understanding of the plant--oomycete pathosystems that is independent of the host, condition, or type of sample by identifying genes of the pathogen expressed in planta.
authors.affiliation: Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá Distrito Capital, Colombia.
authors.forename: Roberto
authors.initials: R
authors.lastname: Sierra
authors.forename: Luis M
authors.initials: LM
authors.lastname: Rodríguez-R
authors.forename: Diego
authors.initials: D
authors.lastname: Chaves
authors.forename: Andrés
authors.initials: A
authors.lastname: Pinzón
authors.forename: Alejandro
authors.initials: A
authors.lastname: Grajales
authors.forename: Alejandro
authors.initials: A
authors.lastname: Rojas
authors.forename: Gabriel
authors.initials: G
authors.lastname: Mutis
authors.forename: Martha
authors.initials: M
authors.lastname: Cárdenas
authors.forename: Daniel
authors.initials: D
authors.lastname: Burbano
authors.forename: Pedro
authors.initials: P
authors.lastname: Jiménez
authors.forename: Adriana
authors.initials: A
authors.lastname: Bernal
authors.forename: Silvia
authors.initials: S
authors.lastname: Restrepo
mesh_terms: D000465:Algorithms; D002874:Chromosome Mapping; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D005821:Genetic Techniques; D018745:Genome, Plant; D023281:Genomics; D008390:Markov Chains; D055750:Phytophthora infestans / Q000235:genetics*