Clinical and serological evaluation of Leptospirosis in Puducherry, India.
BACKGROUND
Leptospirosis is a zoonotic disease with a worldwide distribution. There is a paucity of available data about prevalence of this disease in Pondicherry. Our aim was to investigate the seropositivity rate of leptospirosis in suspected cases and also to identify the predominant serogroups present by performing Microscopic Agglutination Test (MAT). The other aim of this study was to compare the results of a commercially available IgM ELISA with that of MAT.


METHODS
A total of 110 blood samples from patients suspected of leptospirosis were sent for diagnosis. These samples were subjected to IgM ELISA and the microscopic agglutination test (MAT). MAT was done using a panel of 12 Leptospira serovars.


RESULTS
MAT analysis of the 110 samples showed 40 (36%) to be positive. Antibodies were predominantly seen against serogroup Leptospira Icterohemorrhagiae (27%), followed by Pomona (17%), and Pyrogenes (12%). IgM ELISA done on these samples showed a positivity of 37% compared to MAT.


CONCLUSIONS
This study reveals that the MAT test can be standardized in a diagnostic laboratory and used in conjunction with an IgM ELISA.
Shekatkar
Smita Benjamin
SB
Department of Microbiology, Jawaharlal Institute of Post graduate Medical Education and Research, Puducherry, India. smithsmile14@rediffmail.com
Harish
Belgode Narasimha
BN
Menezes
Godfred Antony
GA
Parija
Subhash Chandra
SC
D000293:Adolescent; D000328:Adult; D000368:Aged; D000372:Agglutination Tests / Q000379:methods; D000907:Antibodies, Bacterial / Q000097:blood; D019937:Diagnostic Techniques and Procedures; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007194:India / Q000453:epidemiology; D007919:Leptospira / Q000276:immunology; D007922:Leptospirosis / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies
D000907:Antibodies, Bacterial; D007075:Immunoglobulin M