title: Peripheral blood mononuclear cell population changes associated with age and Mycobacterium avium subspecies paratuberculosis infection in red deer (Cervus elaphus).
abstract: In this study we aimed to document the maturation of the immune system in red deer and investigate the hypothesis that an immature immune system may predispose young red deer (Cervus elaphus) to an increased susceptibility to Johne's disease. Peripheral blood mononuclear cell populations were analysed using flow cytometry techniques to monitor changes associated with age and severity of infection with Mycobacterium avium subspecies paratuberculosis in red deer. The percentage of cells expressing cell population markers CD4, CD8, WC1, gammadelta TCR, CD14 and B-B4 as well as the cell activation markers CD25, CD44, ACT1 and ACT31 were analysed in relation to age and M. avium subsp. paratuberculosis infection status and disease severity. Significant changes in both cell surface markers and cell activation markers were observed as animals matured irrespective of their response to infection. The levels of CD4 and CD8 increased with age and the levels of B-B4, WC1, CD14 and gammadelta TCR decreased similar to previous studies in cattle. No differences were observed in cell surface markers or cell activation markers in relation to M. avium subsp. paratuberculosis infection status and disease severity. This research demonstrates that peripheral blood cell populations change with age in red deer and suggests that studies of cell surface markers and activation markers in peripheral blood samples do not provide information on the age-related susceptibility to Johne's disease.
authors.affiliation: Department of Microbiology and Immunology, University of Otago, 720 Cumberland Street, Dunedin, New Zealand. robma167@student.otago.ac.nz
authors.forename: Mark W
authors.initials: MW
authors.lastname: Robinson
authors.forename: Rory
authors.initials: R
authors.lastname: O'Brien
authors.forename: Colin G
authors.initials: CG
authors.lastname: Mackintosh
authors.forename: J Frank T
authors.initials: JF
authors.lastname: Griffin
mesh_terms: D000367:Age Factors; D000818:Animals; D003670:Deer / Q000276:immunology / Q000382:microbiology*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D016130:Immunophenotyping / Q000662:veterinary*; D007410:Intestinal Diseases / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology*; D008213:Lymphocyte Activation / Q000276:immunology*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D010283:Paratuberculosis / Q000276:immunology / Q000382:microbiology*