title: An outbreak of Burkholderia cenocepacia bacteremia in immunocompromised oncology patients.
abstract: BACKGROUND
  Burkholderia cepacia is a common environmental bacterium that is resistant to disinfectants, and therefore is often encountered as a hospital-acquired pathogen. We describe an outbreak of B. cenocepacia bacteremia among hospitalized oncology patients.
  
  
  METHODS
  A matched case-control study and an extensive environmental investigation were conducted. Species were identified by RFLP of the amplified recA gene. DNA was fingerprinted by pulsed-field gel electrophoresis (PFGE).
  
  
  RESULTS
  Between November 2005 and September 2006, B. cenocepacia bacteremia developed in 17 patients with underlying malignancy of whom 14 had tunneled central venous catheters. All patients had fever and chills which subsided following removal of the central catheter and administration of ceftazidime. Extensive epidemiological investigation could not find a common source for the outbreak. Patients were hospitalized in three different buildings with different health care personnel. Medications were prepared in different sites by different personnel. A multivariate analysis demonstrated that the independent risk factors for developing nosocomial B. cenocepacia bacteremia were hospitalization at the center for long-term support (OR 28.8; 95% CI 1.83-453.4) and reduced use of antibiotics during the last month (OR 0.07; 95% CI 0.01-0.40). All isolates had identical antimicrobial susceptibility; PFGE indicated that a complex of closely related strains was involved in the outbreak. All isolates were identified as B. cenocepacia, known to infect cystic fibrosis patients. Strict infection control measures terminated the outbreak.
  
  
  CONCLUSIONS
  B. cenocepacia is an emerging nosocomial pathogen among oncology patients.
authors.affiliation: Infectious Diseases Unit, Sheba Medical Center, 52621, Tel-Hashomer, Israel.
authors.forename: T
authors.initials: T
authors.lastname: Mann
authors.forename: D
authors.initials: D
authors.lastname: Ben-David
authors.forename: A
authors.initials: A
authors.lastname: Zlotkin
authors.forename: D
authors.initials: D
authors.lastname: Shachar
authors.forename: N
authors.initials: N
authors.lastname: Keller
authors.forename: A
authors.initials: A
authors.lastname: Toren
authors.forename: A
authors.initials: A
authors.lastname: Nagler
authors.forename: G
authors.initials: G
authors.lastname: Smollan
authors.forename: A
authors.initials: A
authors.lastname: Barzilai
authors.forename: G
authors.initials: G
authors.lastname: Rahav
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016470:Bacteremia / Q000453:epidemiology / Q000276:immunology* / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics; D019117:Burkholderia / Q000302:isolation & purification; D019121:Burkholderia Infections / Q000453:epidemiology / Q000276:immunology*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006757:Hospital Units; D006801:Humans; D016867:Immunocompromised Host; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009369:Neoplasms / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D011938:Rec A Recombinases / Q000235:genetics; D012307:Risk Factors
chemical_list: D001426:Bacterial Proteins; D011938:Rec A Recombinases