Advances in molecular detection of Aspergillus: an update.
Filamentous cosmopolitan fungi of the genus Aspergillus can be harmful in two ways, directly they can be opportunistic pathogens causing aspergillosis and indirectly due to aflatoxin production on food products which can lead to aflatoxicosis. Therefore, a number of methods have been proposed so far for detection of the fungi with lowest possible concentration at the earliest. Molecular methods such as PCR and/or in combination with certain techniques have been found to be useful for Aspergillus detection. We discuss here various technologies that have emerged in recent years and can possibly be used for the molecular detection of Aspergillus in an efficient way. These methods like RSIC, C-probe, and inversion probe with pyrosequencing or direct ss/dsDNA detection have been used for the identification of fungal or bacterial pathogens and thus formulate a 'gold standard' for Aspergillus detection.
Abdin
M Z
MZ
Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi, 110062, India.
Ahmad
Malik M
MM
Javed
Saleem
S
D000348:Aflatoxins / Q000378:metabolism; D000818:Animals; D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology; D001230:Aspergillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D005516:Food Microbiology; D005821:Genetic Techniques; D016533:Mycological Typing Techniques / Q000379:methods*; D009172:Mycology / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D018626:Retroelements
D000348:Aflatoxins; D004271:DNA, Fungal; D018626:Retroelements