[Isolation of a PHA producing strain with butyric acid as the carbon source and its shaking-flask fermentation character].
A bacterial strain,WD-3,with high polyhydroxyalkanoates (PHA) yield was isolated from activated sludge. Its fermentation characters including the effects of C:N ratio, pH,fermentation time on the PHA production were also primary studied. The results showed that the optimal C/N ratio and pH were 35 and 7.0, respectively. PHA accumulation increased rapidly during the stable growth stage. The maximum PHA yield was 3.01 g x L(-), the strain accumulated PHA up to 45.4% of its cellular dry weight, and the proportion of PHV occupied one-third of the PHA production. Analysis of its 16S rRNA sequence showed that strain WD-3 belongs to Enterobacter.
Chen
Zhi-qiang
ZQ
National Engineering Research Center of Urban Water Resources, Harbin 150090, China. czq0521@tom.com
Li
Yun-bei
YB
Wen
Qin-xue
QX
D020148:Butyric Acid / Q000378:metabolism*; D004754:Enterobacter / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D005285:Fermentation; D007220:Industrial Waste; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D013025:Glycine max
D007220:Industrial Waste; D054813:Polyhydroxyalkanoates; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D020148:Butyric Acid