Human atrial natriuretic peptide ameliorates LPS-induced acute lung injury in rats.
Acute lung injury, a common component of systemic inflammatory disease, is a life-threatening condition without many effective treatments. However, recent studies have demonstrated that the multifunctional human atrial natriuretic peptide (hANP) exerts anti-inflammatory effects. Therefore, we tested the hypothesis that hANP could prevent lipopolysaccharide (LPS)-induced acute lung injury in a rodent model. Rats received an LPS injection and continuous intravenous infusion (CIV) of hANP or saline solution. We evaluated the anti-inflammatory effects of hANP by histological examination and determination of serum cytokine levels and lung myeloperoxidase (MPO) activity. Histological examination revealed marked reductions in interstitial congestion, edema, inflammation, and hemorrhage in lung tissue harvested 12 h after hANP treatment compared with tissue from rats that received saline treatment after LPS. LPS injection induced elevated cytokine (IL-1beta and IL-6) secretion and lung MPO activity, which was also attenuated by hANP treatment. Taken together, these data demonstrate that hANP exerts an anti-inflammatory effect and may have potential as a therapeutic agent to treat systemic inflammatory diseases.
Koga
Hironori
H
Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, Oita University, 1-1 Idaigaoka-Hasamamachi, Yufu City, Oita, 879-5593, Japan. h.koga@med.oita-u.ac.jp
Hagiwara
Satoshi
S
Shingu
Chihiro
C
Matsumoto
Shigekiyo
S
Yokoi
Isao
I
Noguchi
Takayuki
T
D055371:Acute Lung Injury / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D007262:Infusions, Intravenous; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides; D008297:Male; D009195:Peroxidase / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D009320:Atrial Natriuretic Factor; D009195:Peroxidase