CMTM5-v1, a four-transmembrane protein, presents a secreted form released via a vesicle-mediated secretory pathway.
The CKLF-like MARVEL transmembrane domain-containing family (CMTM) is a novel family of proteins linking classical chemokines and the transmembrane 4 superfamily (TM4SF). Our earlier studies indicated several CMTM members (such as CKLF1 and CMTM2) have a secreted form. This is the first report of the secreted form of CMTM5-v1, the major RNA splicing form of CMTM5, which is produced as small vesicles (100 nm diameter) and floats at a peak density of 1.19 g/ml on continuous sucrose gradients. CMTM5-v1 has no obvious co-localization with CD63 or Golgi complex. In addition, brefeldin A but not wortmannin can inhibit the secretion of CMTM5-v1. Our results suggest that CMTM5-v1 might be secreted via a different vesicle-mediated secretory pathway, which will be helpful for the studies of vesicle-mediated secretion and MARVEL domain-containing proteins.
Li
Henan
H
Center for Human Disease Genomics, Department of Immunology, School of Basic Medical Sciences, Health Science Center, Peking University, 38 Xueyuan Road, Beijing 100191, China.
Guo
Xiaohuan
X
Shao
Luning
L
Plate
Markus
M
Mo
Xiaoning
X
Wang
Yu
Y
Han
Wenling
W
D000730:Androstadienes / Q000494:pharmacology; D015703:Antigens, CD / Q000378:metabolism; D020126:Brefeldin A / Q000494:pharmacology; D018925:Chemokines / Q000737:chemistry / Q000378:metabolism*; D006056:Golgi Apparatus; D006801:Humans; D062792:MARVEL Domain-Containing Proteins; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism; D012326:RNA Splicing; D055571:Secretory Pathway; D022142:Secretory Vesicles / Q000378:metabolism*; D060149:Tetraspanin 30; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000378:metabolism*; D000077191:Wortmannin
D000730:Androstadienes; D015703:Antigens, CD; C554586:CD63 protein, human; C525020:CMTM5 protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; D025521:Tumor Suppressor Proteins; D020126:Brefeldin A; D000077191:Wortmannin