title: Comparative analysis of DNA methylation in transgenic mice with unstable CGG repeats from FMR1 gene.
abstract: Methylation of CpG sequences in and around CGG triplet repeats in FMR1 gene has strong correlation with manifestation of the fragile X syndrome in human patients. In contrast, we have observed a lack of correlation between repeat instability and DNA methylation in three different transgenic mouse models harboring unstable CGG repeats. Further we have demonstrated that the endogenous copy of mouse Fmr1 gene remains unmethylated both in males and females. These results imply that methylation and repeat instability are independent events and raise the possibility that methylation could also result in repression of FMR1 transcription in the absence of repeat expansion.
authors.affiliation: University of Delhi.
authors.forename: Mohammad Parwez
authors.initials: MP
authors.lastname: Alam
authors.affiliation: University of Delhi.
authors.forename: Sonal
authors.initials: S
authors.lastname: Datta
authors.affiliation: National Institute of Immunology, Delhi.
authors.forename: Subeer
authors.initials: S
authors.lastname: Majumdar
authors.affiliation: University of Delhi.
authors.forename: Abhishek K
authors.initials: AK
authors.lastname: Mehta
authors.affiliation: University of Delhi.
authors.forename: Sujatha
authors.initials: S
authors.lastname: Baskaran
authors.affiliation: National Institute of Immunology, Delhi.
authors.forename: Neerja
authors.initials: N
authors.lastname: Gulati
authors.affiliation: University of Delhi.
authors.forename: Vani
authors.initials: V
authors.lastname: Brahmachari
mesh_terms: D000818:Animals; D019175:DNA Methylation / Q000235:genetics*; D043243:DNA Sequence, Unstable; D005260:Female; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008822:Mice, Transgenic; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D051860:Fragile X Messenger Ribonucleoprotein 1; C497683:FMR1 protein, human; C497684:Fmr1 protein, mouse