Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry.
Uric acid is a potent antioxidant and scavenger of singlet oxygen and other radicals in humans. Allantoin, the predominant product of free radical-induced oxidation of uric acid, is efficiently excreted in the urine and has potential as a biomarker of oxidative stress. We developed a rapid and specific assay for urinary allantoin using ultra-performance liquid chromatography-tandem mass spectrometry suitable for high-throughput clinical studies. The method required minimal sample preparation and was accurate (mean error=6%), precise (intra- and interday imprecision <8%), and sensitive (limit of detection=0.06pmol). Allantoin levels measured in control samples were comparable to literature values.
Tolun
Adviye A
AA
Department of Pediatrics, Duke University Medical Center, Durham NC 27709, USA.
Zhang
Haoyue
H
Il'yasova
Dora
D
Sztáray
Judit
J
Young
Sarah P
SP
Millington
David S
DS
D000293:Adolescent; D000328:Adult; D000368:Aged; D000481:Allantoin / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*; D055815:Young Adult
D000481:Allantoin