title: RANK/RANKL expression is induced by cardiac surgical operation.
abstract: BACKGROUND
  Cardiac surgery provokes a systemic inflammatory response in any patient. This complex body reaction involves also RANK/RANKL molecules which have been recently identified as principal regulators of bone metabolism.
  
  
  OBJECTIVE
  To follow the changes in the expression of RANK/RANKL molecules on innate immune cells of cardiac surgical patients.
  
  
  METHODS
  Twenty-six patients undergoing cardiac surgical were assigned to undergo coronary artery bypass grafting using either cardiopulmonary bypass ("on-pump") or modified "miniinvasive on-pump". The expression of RANK/RANKL was performed by flow cytometry.
  
  
  RESULTS
  Significantly increased expression of RANK on monocytes of "miniinvasive on-pump" patients was found at the 1st, the 3nd, and 7th postoperative days. The similar pattern was found also for monocyte RANKL expression. In addition, RANKL expression was significantly increased at the 3rd postoperative day in "on-pump" patient. No significant differences between "miniinvasive on-pump" and "on-pump" cardiac surgical patients were found.
  
  
  CONCLUSIONS
  The expression of both RANK and RANKL molecules is significantly enhanced on monocytes of "miniinvasive on-pump" cardiac surgical patients.
authors.affiliation: Department of Clinical Immunology and Allergology, Charles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Czech Republic. krejsek@fnhk.cz
authors.forename: Jan
authors.initials: J
authors.lastname: Krejsek
authors.forename: Martina
authors.initials: M
authors.lastname: Kolácková
authors.forename: Vladimír
authors.initials: V
authors.lastname: Lonský
authors.forename: Manuela Trojácková
authors.initials: MT
authors.lastname: Kudlová
authors.forename: Jirí
authors.initials: J
authors.lastname: Mandák
authors.forename: Pavel
authors.initials: P
authors.lastname: Kunes
authors.forename: Karolina
authors.initials: K
authors.lastname: Jankovicová
authors.forename: Dana
authors.initials: D
authors.lastname: Vlásková
authors.forename: Andrýs
authors.initials: A
authors.lastname: Ctirad
mesh_terms: D000368:Aged; D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass; D005260:Female; D005434:Flow Cytometry; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D008297:Male; D009000:Monocytes / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism*; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000378:metabolism*
chemical_list: D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B