Label-free and naked eye detection of PNA/DNA hybridization using enhancement of gold nanoparticles.
Utilizing a gold enhancement process after inducing electrostatic interaction between positively charged gold nanoparticles and negatively charged target DNA hybridized to neutral PNA capture probes, a new method for label-free detection of DNA was developed and successfully applied to detect H5-type DNA.
Kim
Sang Kyu
SK
BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, Republic of Korea.
Cho
Hyunmin
H
Jeong
Jinyoung
J
Kwon
Ji Na
JN
Jung
Yongwon
Y
Chung
Bong Hyun
BH
D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D017403:In Situ Hybridization; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
D020135:Peptide Nucleic Acids; D006046:Gold; D004247:DNA