title: The transDSL ligase ribozyme can utilize various forms of modules to clamp its substrate and enzyme units.
abstract: TransDSL is an RNA ligase ribozyme whose enzyme unit joins two RNA fragments constituting a substrate. The enzyme unit recognizes the substrate by means of two clamp modules. We constructed active variants by replacing the original clamp module with various types of interactions. Such flexible modularity would be advantageous in the application of this ribozyme in nanobiotechnology.
authors.affiliation: Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: Junya
authors.initials: J
authors.lastname: Ishikawa
authors.forename: Nana
authors.initials: N
authors.lastname: Isomoto
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fujita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Furuta
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Ikawa
mesh_terms: D008025:Ligases / Q000235:genetics; D012313:RNA / Q000235:genetics; D011130:RNA Ligase (ATP) / Q000235:genetics / Q000378:metabolism; D016337:RNA, Catalytic / Q000235:genetics* / Q000378:metabolism*
chemical_list: D016337:RNA, Catalytic; D012313:RNA; D008025:Ligases; D011130:RNA Ligase (ATP)