[Effect of copper intake and copper-zinc ratio on rat lipid peroxidation in copper deficiency].
OBJECTIVE
To study the relations among the copper concentration, the copper-zinc ratio (Cu/Zn) and the biomarkers of lipid peroxidation by controlling the level of copper intake in copper deficiency, to analyze the effect of trace element copper on the rat lipid peroxidation.


METHODS
Determine the content of copper in blood and the activity of superoxide dismutase (SOD), glutathione peroxidase (QSH-Px) and catalase (CAT) and the content of malondialdehyde (MDA), carrying out the relevant research.


RESULTS
The rats were treated with copper gluconate (Cu-G) in different doses in the case of insufficient copper intake, the copper concentration in the blood of each group rats presented irregular fluctuate, while the copper-zinc ratio (Cu/Zn) showed a ascending trend following the increase of copper intake, and the results have statistical significance (P < 0.01). In the status of copper deficiency, the SOD activity is lower than the normal level, and then presented a rise trend with the increased of copper intake that is, the ratio of copper-zinc (Cu/Zn). The content of MDA is higher than the normal level, and the content of MDA decreased with the increased of copper intake and the Cu/Zn ratio, and maintained at a relatively low level. The decreased of Cu/Zn ratio could induce the decrease of the CAT activity in the rat blood, while there were no linear correlation be found between them. And no significant effect on the GSH-Px activity in the rat blood in inadequate of copper intake.


CONCLUSIONS
In copper deficiency, the SOD activity in the rat blood would be lower than the normal level and the content of MDA would increase in rat blood, while with the increase of Cu/Zn ratio, the SOD activity increased gradually, and the content of MDA dropped to a lowed level. While the insufficient intake of copper could induce a increase of the CAT activity in blood and no effect on GSH-Px.
Duan
Lian
L
Institute for Environmental Health and Related Product Safety, China CDC, Beijing, China.
Cheng
Yibing
Y
Jin
Yinlong
Y
D000818:Animals; D002374:Catalase / Q000097:blood; D003300:Copper / Q000008:administration & dosage* / Q000097:blood / Q000172:deficiency*; D005260:Female; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood; D015032:Zinc / Q000097:blood*
D003300:Copper; D002374:Catalase; D013482:Superoxide Dismutase; D015032:Zinc