title: The enhanced transcriptional activity of the V-val subtype of Epstein-Barr virus nuclear antigen 1 in epithelial cell lines.
abstract: The Epstein-Barr virus encoded nuclear antigen 1 (EBNA1) is required for the replication and maintenance of the episomal EBV genome and for the transactivation of viral gene expression. EBNA1 has been classified into five subtypes, among which the V-val subtype was reported to be associated with nasopharyngeal carcinoma (NPC). Here we report a higher transcriptional activity of the V-val subtype of EBNA1 than for the prototype derived from B95.8 cells to transactivate FR-containing luciferase plasmid, which was mainly a consequence of the mutations in the carboxy-terminus of EBNA1. This interpretation was further supported by the finding that the variant form of EBNA1 has a higher binding affinity for the FR sequence than the prototype by electrophoretic mobility shift assays. The functional advantage of the V-val EBNA1 investigated in this study may contribute to the oncogenesis of NPC.
authors.affiliation: Department of Experimental Research, State Key Laboratory of Oncology in Southern China, Cancer Center, Sun Yat-sen University, Guangzhou 510060, P.R. China.
authors.forename: Shi-Juan
authors.initials: SJ
authors.lastname: Mai
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.forename: Yu-Fan
authors.initials: YF
authors.lastname: Huang
authors.forename: Feng-Wei
authors.initials: FW
authors.lastname: Wang
authors.forename: Yi-Ji
authors.initials: YJ
authors.lastname: Liao
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Deng
authors.forename: Wen-Ju
authors.initials: WJ
authors.lastname: Liu
authors.forename: Wen-Feng
authors.initials: WF
authors.lastname: Hua
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Zeng
mesh_terms: D001665:Binding Sites; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D004847:Epithelial Cells / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D009154:Mutation; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000821:virology; D013997:Time Factors; D015533:Transcriptional Activation; D014162:Transfection
chemical_list: D019309:Epstein-Barr Virus Nuclear Antigens; D004247:DNA; D008156:Luciferases; C417029:EBV-encoded nuclear antigen 1