Strobilanthes crispus attenuates renal carcinogen, iron nitrilotriacetate (Fe-NTA)-mediated oxidative damage of lipids and DNA.
This study was aimed to evaluate the effect of Strobilanthes crispus extract for possible protection against lipid peroxidation and DNA damage induced by iron nitrilotriacetate (Fe-NTA) and hydrogen peroxide (H(2)O(2)). Fe-NTA is a potent nephrotoxic agent and induces acute and subacute renal proximal tubular necrosis by catalyzing the decomposition of H(2)O(2)-derived production of hydroxyl radicals, which are known to cause lipid peroxidation and DNA damage. Incubation of postmitochondrial supernatant and/or calf thymus DNA with H(2)O(2) (40 mM) in the presence of Fe-NTA (0.1 mM) induces lipid peroxidation and DNA damage to about 2.3-fold and 2.9-fold, respectively, as compared to control (P < 0.05). In lipid peroxidation protection studies, S. crispus treatment showed a dose-dependent inhibition (45-53% inhibition, P < 0.05) of Fe-NTA and H(2)O(2) induced lipid peroxidation. Similarly, in DNA damage protection studies, S. crispus treatment also showed a dose-dependent inhibition (18-30% inhibition, P < 0.05) of DNA damage. In addition, the protection was closely related to the content of phenolic compounds as evident by S. crispus extract showing the value of 124.48 mg/g total phenolics expressed as gallic acid equivalent (GAE, mg/g of extract). From these studies, it is concluded that S. crispus inhibits peroxidation of membrane lipids and DNA damage induced by Fe-NTA and H(2)O(2) and possesses the potential to be used to treat or prevent degenerative diseases where oxidative stress is implicated.
Iqbal
Mohammad
M
Biotechnology Research Institute, University Malaysia Sabah, Locked Bag No 2073, 88999 Kota Kinabalu, Sabah, Malaysia. miqbal2k2008@hotmail.com
Shah
Muhammad Dawood
MD
Lie
Chau Ann
CA
San
Chin Kim
CK
D002273:Carcinogens / Q000633:toxicity; D004249:DNA Damage / Q000187:drug effects*; D005290:Ferric Compounds / Q000633:toxicity; D006861:Hydrogen Peroxide; D007668:Kidney / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects*; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*
D002273:Carcinogens; D005290:Ferric Compounds; D010636:Phenols; D010936:Plant Extracts; D020011:Protective Agents; D006861:Hydrogen Peroxide; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate