title: Characterization of novel porcine sapoviruses.
abstract: Sapoviruses are common caliciviruses known to cause enteric diseases in humans and animals. SaVs are genetically highly heterogeneous and are presently classified in five genogroups that are further subdivided in a number of genotypes. In recent years, a number of novel animal SaV strains, mostly of swine origin, have been partially characterized and proposed to represent novel genogroups or genotypes. We previously reported the detection and partial characterization of a wide range of variable and novel SaV strains of uncertain taxonomic status in Canadian swine. We now report on further genomic characterization of two novel strains to clarify their taxonomic relationship to other swine and human SaVs. Detailed analysis of different regions of their genomes, including determination of their complete capsid sequence, did not permit clear taxonomic assignment according to current criteria. This situation appears reminiscent of that of a number of SaV strains of swine origin and calls for a classification update for this calicivirus genus. We also report the detection of swine GIII SaVs for the first time in Canada.
authors.affiliation: St-Hyacinthe Laboratory, Canadian Food Inspection Agency, 3400 Blvd. Casavant West, Saint-Hyacinthe, Quebec, Canada. yvan.lhomme@inspection.gc.ca
authors.forename: Yvan
authors.initials: Y
authors.lastname: L'Homme
authors.forename: Julie
authors.initials: J
authors.lastname: Brassard
authors.forename: Mourad
authors.initials: M
authors.lastname: Ouardani
authors.forename: Marie-Josée
authors.initials: MJ
authors.lastname: Gagné
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D001483:Base Sequence; D017250:Caliciviridae Infections / Q000662:veterinary* / Q000821:virology; D002213:Capsid / Q000737:chemistry; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000662:veterinary* / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D029341:Sapovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000821:virology*