Insertional mutagenesis reveals genes involved in Bacillus cereus ATCC 14579 growth at low temperature.
Transposon mutagenesis of Bacillus cereus ATCC 14579 yielded cold-sensitive mutants. Mutants of genes encoding enzymes of the central metabolism were affected by cold, but also by other stresses, such as pH or salt, whereas a mutant with transposon insertion in the promoter region of BC0259 gene, encoding a putative DEAD-box RNA helicase displaying homology with Escherichia coli CsdA and Bacillus subtilis CshA RNA helicases, was only cold-sensitive. Expression of the BC0259 gene at 10 degrees C is reduced in the mutant. Analysis of the 5' untranslated region revealed the transcriptional start and putative cold shock-responsive elements. The role of this RNA helicase in the cold-adaptive response of B. cereus is discussed.
Broussolle
Véronique
V
INRA, UMR Sécurité et Qualité des Produits d'Origine Végétale, Avignon, France. broussol@avignon.inra.fr <broussol@avignon.inra.fr>
Pandiani
Franck
F
Haddad
Nabila
N
Michaud
Caroline
C
Carlin
Frédéric
F
Nguyen-the
Christophe
C
Brillard
Julien
J
D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D001409:Bacillus cereus / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology / Q000528:radiation effects; D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D003080:Cold Temperature; D053487:DEAD-box RNA Helicases / Q000235:genetics; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics; D017353:Gene Deletion; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D020365:RNA Helicases / Q000235:genetics / Q000502:physiology*; D017385:Sequence Homology; D013312:Stress, Physiological; D024363:Transcription Initiation Site
D020121:5' Untranslated Regions; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; C088840:deaD protein, E coli; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases