title: Detection and quantification of some plant growth regulators in a seaweed-based foliar spray employing a mass spectrometric technique sans chromatographic separation.
abstract: The sap expelled from the fresh harvest of Kappaphycus alvarezii , a red seaweed growing in tropical waters, has been reported to be a potent foliar spray. Tandem mass spectrometry of various organic extracts of the sap confirmed the presence of the plant growth regulators (PGRs) indole 3-acetic acid, gibberellin GA(3), kinetin, and zeatin. These PGRs were quantified in fresh state and after 1 year of storage by ESI-MS without recourse to chromatographic separation. Quantification was validated against HPLC data. The results may be useful in correlating with the efficacy of the sap. The methodology was extended to two other seaweeds. The method developed is convenient and precise and may find application in other agricultural formulations containing these growth hormones.
authors.affiliation: Central Salt and Marine Chemicals Research Institute (Council of Scientific and Industrial Research), G. B. Marg, Bhavnagar, Gujarat, India. kamlesh@csmcri.org
authors.forename: Kamalesh
authors.initials: K
authors.lastname: Prasad
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Das
authors.forename: Mihir Deepak
authors.initials: MD
authors.lastname: Oza
authors.forename: Harshad
authors.initials: H
authors.lastname: Brahmbhatt
authors.forename: Arup Kumar
authors.initials: AK
authors.lastname: Siddhanta
authors.forename: Ramavatar
authors.initials: R
authors.lastname: Meena
authors.forename: Karuppanan
authors.initials: K
authors.lastname: Eswaran
authors.forename: Mahesh Rameshchandra
authors.initials: MR
authors.lastname: Rajyaguru
authors.forename: Pushpito Kumar
authors.initials: PK
authors.lastname: Ghosh
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010937:Plant Growth Regulators / Q000032:analysis*; D012624:Seaweed / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010937:Plant Growth Regulators