Expression and significance of FXYD-3 protein in gastric adenocarcinoma.
OBJECTIVE
FXYD-3, also known as Mat-8, is a member of the FXYD protein family. It was reported that this protein can associate with and modify the transport properties of Na, K-ATPase, and may play an important role in a variety of physiological and pathological states. This protein is up-regulated in certain types of cancers (such as breast, prostate and pancreatic cancer), but down-regulated in other types of cancers (such as colon and kidney cancer). No study has been performed in gastric cancer; therefore, the aim of this project was to investigate FXYD-3 expression and its clinicopathological significance in gastric adenocarcinoma.


METHODS
FXYD-3 protein was examined by immunohistochemistry in normal gastric mucous (n= 29) and gastric adenocarcinoma (n=51), obtained from surgical resection of gastric cancer patients.


RESULTS
FXYD-3 protein was present in the cytoplasm of normal gastric epithelial cells or gastric cancer cells. The rate of FXYD-3 strong expression was significantly higher in cancer (51% of 51) than in normal mucosa (10% of 29, X<formula>;{2}</formula>=13.210, p < 0.0001). FXYD-3 expressed strongly in ulcerative/infiltrating types of cancers compared to polypoid/fungating ones (X<formula>;{2}</formula>=5.765, p=0.016). However, FXYD-3 expression was not correlated with patient's gender, age, tumor size, lymph node status and histological grade (p > 0.05). Conclosion: Up-regulated expression of FXYD-3 protein may be involved in tumourgenesis and invasion of gastric adenocarcinoma.
Zhu
Zhen-Long
ZL
Center of Scientific Research, The First Hospital of Hebei Medical University, Shijiazhuang, China Department of Pathology, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Zhao
Zeng-Ren
ZR
Zhang
Yu
Y
Yang
Yan-Hong
YH
Wang
Zheng-Min
ZM
Cui
Dong-Sheng
DS
Wang
Ming-Wei
MW
Kleeff
Jörg
J
Kayed
Hany
H
Yan
Bao-Yong
BY
Sun
Xiao-Feng
XF
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology / Q000556:secondary; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
C091777:FXYD3 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D000254:Sodium-Potassium-Exchanging ATPase