title: Spectrometric studies on the sonodynamic damage of protein in the presence of levofloxacin.
abstract: Taking bovine serum albumin (BSA) as typical molecules, the sonodynamic damage of protein in the presence of Levofloxacin (LVFX) and its mechanism were studied by fluorescence and UV-vis spectra. Various influencing factors such as ultrasonic irradiation time, pH value, ionic strength and solution temperature on the damage of BSA were also discussed. The results showed that ultrasound can enhance the damage of LVFX on BSA. The damage degree of BSA was aggravated with the increase of ultrasonic irradiation time, solution temperature and ionic strength, whereas decreased with the increase of solution pH value. Furthermore, the reactive oxygen species (ROS) in reaction system were studied by oxidation and extraction photometry. Experimental results showed that the amounts of superoxide anion radical (·O(2)(-)) and hydroxyl radical (·OH) were significantly more than that of singlet oxygen ((1)O(2)) in the presence of LVFX under ultrasonic irradiation.
authors.affiliation: Department of Chemistry, Northeastern University, Shenyang, 110004, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Bing-Mi
authors.initials: BM
authors.lastname: Liu
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Kong
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Xu
mesh_terms: D000818:Animals; D002417:Cattle; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical; D064704:Levofloxacin; D015242:Ofloxacin / Q000737:chemistry*; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013057:Spectrum Analysis / Q000379:methods*; D013481:Superoxides; D013696:Temperature; D014465:Ultrasonics
chemical_list: D017382:Reactive Oxygen Species; D013481:Superoxides; D012710:Serum Albumin, Bovine; D017665:Hydroxyl Radical; D064704:Levofloxacin; D015242:Ofloxacin