Uptake of functionalized mesoporous silica nanoparticles by human cancer cells.
Mesoporous silica nanoparticles (MSN) were functionalised by aminofluorescein (AMF) with diethylenetriaminepentaacetic acid spacer molecules which provide free carboxylic groups for binding cell-specific ligands such as folate. AMF allowed the exploration of cellular uptake by HeLa cells using confocal microscopy and flow cytometry. The functionalized nanoparticles (MSN-AMF) penetrated efficiently into HeLa cell cytoplasm through a clathrin dependent endocytosis mechanism. The number of endocytosed MSN-AMF was enhanced when using folate as a targeting molecule. Uptake kinetics revealed that most of MSN-AMF were internalized within 4 h of incubation. Moreover, we found that MSN-AMF were capable of escaping the acidic endolysosomal vesicles of HeLa cells. Cytotoxicity studies suggested that these nanoparticles are non-toxic to HeLa cells up to a dose level of 50 microg/ml.
Fisichella
Matthieu
M
Centre de Recherche sur la Matière Divisée-UMR6619 Université d'Orléans-CNRS, 1B rue de la Férollerie, 45071 Orleáns Cedex 2, France.
Dabboue
Hinda
H
Bhattacharyya
Sanjib
S
Lelong
Gérald
G
Saboungi
Marie-Louise
ML
Warmont
Fabienne
F
Midoux
Patrick
P
Pichon
Chantal
C
Guérin
Martine
M
Hevor
Tobias
T
Salvetat
Jean-Paul
JP
D003460:Crystallization / Q000379:methods*; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
D046911:Macromolecular Substances; D012822:Silicon Dioxide