title: [Pressurized liquid extraction of Tripterygiumw wilfordii polyglycosides and optimization of extraction conditions].
abstract: OBJECTIVE
  To investigate the extraction method of polyglycosides from Tripterygium wilfordii.
  
  
  METHODS
  The extraction method of pressurized liquid extraction was employed and chromogenic colorimetric technique were used for quantitative analysis. Based on the single-factor experiment, according to the center combination design this paper used three factors and three levels of response surface methodology for process optimization.
  
  
  RESULTS
  The optimized conditions were as follows: ratio of solid to liquid was 1:9.5, at the temperature of 115 degrees C for 80 minutes, the actual extract ratio and purity of polyglycosides obtained were 0.21% and 0.52%, respectively.
  
  
  CONCLUSIONS
  The method of pressurized liquid extraction has obvious advantages over conventional reflux extracting.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Tong
authors.initials: T
authors.lastname: Li
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zu
mesh_terms: D006027:Glycosides / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D011312:Pressure; D029757:Tripterygium / Q000737:chemistry*
chemical_list: D006027:Glycosides; D010936:Plant Extracts