Acyl modified chitosan derivatives for oral delivery of insulin and curcumin.
In the present investigation, bioadhesive property of chitosan (CS) was enhanced by the N-acylation with hexanoyl, lauroyl and oleoyl chlorides. The chemical structure of the modified polymer was characterized by FTIR and zeta potential measurements. The swelling ability was evaluated at alkaline pH. Mucin interactions and mucoadhesion experiments were performed under in vitro experimental conditions. Cytotoxicity experiments were employed to confirm the applicability of these particles as drug carriers. Finally in vitro evaluation of hydrophobic and hydrophilic drug release profile at acidic and alkaline pH was also conducted. A strong interaction between CS acyl derivatives and mucin was detected, which was further confirmed by an in situ mucoadhesion experiments with excised intestinal tissue. CS modified with oleoyl chloride showed better mucoadhesion property, as compared to the one modified with lower fatty acid groups. CS derivatives were found non-toxic on L-929 cell lines and provided sustained release of hydrophobic drugs under in vitro experimental conditions. From these studies it seems that hydrophobically modified CS is an interesting system for drug delivery applications.
Shelma
R
R
Division of Biosurface Technology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695012, India.
Sharma
Chandra P
CP
D000215:Acylation; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D006728:Hormones; D007328:Insulin / Q000008:administration & dosage* / Q000737:chemistry; D009077:Mucins / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
D004337:Drug Carriers; D006728:Hormones; D007328:Insulin; D009077:Mucins; D011108:Polymers; D048271:Chitosan; D003474:Curcumin