[Recent advances in enzyme assays using fluoremetry].
Enzymes play such a pivotal role in cellular metabolism that enzyme assays are important for bio-engineering, disease diagnoses and drug discovery. Among the reported methods, fluoremetry has attracted more and more attention due to its high sensitivity and possibility of continuous dynamic monitoring. The recent progresses and applications in enzyme assays using fluorescent probes were reviewed. Different methods were classified into direct fluorescence detection and indirect fluorescence detection according to their labeled substrates and detection mechanisms. Our writing purpose is to provide the readers with a flavor of the kinds of tools and strategies available in enzyme assays with fluorescent probes. Also, the research situation and prospects were disucssed
Xing
Yanlong
Y
Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Science, Qingdao 266101, China.
Mao
Xiangzhao
X
Wang
Shu
S
Wang
Hualei
H
Wei
Dongzhi
D
D000818:Animals; D057075:Enzyme Assays / Q000379:methods* / Q000639:trends*; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence
D005456:Fluorescent Dyes