Use of the hemagglutinating virus of Japan (HVJ) envelope as a versatile delivery system for nucleic acids and proteins to leukocytes in vitro.
The hemagglutinating virus of Japan (HVJ; also called the Sendai virus) envelope has been developed as a safe and efficient non-viral vector. Because replication and transcription of genomic RNA is inactivated by beta-propiolactone treatment or UV-irradiation, the HVJ envelope is extremely safe. This unit describes the method of transfection of siRNA and protein with the HVJ envelope.
Kondo
Yoshitaka
Y
Life Science Research Laboratory, Central Research Institute, Ishihara Sangyo Kaisha, Ltd., Kusatsu, Shiga, Japan.
Miyata
Keizo
K
Life Science Research Laboratory, Central Research Institute, Ishihara Sangyo Kaisha, Ltd., Kusatsu, Shiga, Japan.
Kato
Fuminori
F
Life Science Research Laboratory, Central Research Institute, Ishihara Sangyo Kaisha, Ltd., Kusatsu, Shiga, Japan.
D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism*; D029082:Sendai virus / Q000737:chemistry*; D014162:Transfection / Q000379:methods*; D014759:Viral Envelope Proteins / Q000378:metabolism*
D011506:Proteins; D034741:RNA, Small Interfering; D014759:Viral Envelope Proteins