title: A study of the effects of chromium exposure on the growth of Pseudokirchneriella subcapitata (Korshikov) hindak evaluated by Central Composite Design and Response Surface Methodology.
abstract: The aim of this study was to evaluate the effects of chromium exposure on the growth of P. subcapitata using the Central Composite Design (CCD) and Response Surface Methodology (RSM). The highest values for algal density and biomass were obtained in the longest exposure times and for the lowest chromium concentrations. The CCD used for the analysis of treatment combinations showed that a second order polynomial regression model was in good agreement with experimental results, with R (2) = 81.50 and 89.90; for algal density and biomass (p < 0.05), respectively. Only the exposure time was significant for algal density. For chlorophyll, in contrast, the exposure time, chromium concentration and their interaction significantly affected the growth of P. subcapitata. The findings confirmed the sensitivity of P. subcapitata to chromium (VI), which makes it a suitable bioindicator of environmental contamination for this metal.
authors.affiliation: Ecophysiology Laboratory for Aquatic Organisms, Research Center on Water Resources and Applied Ecology, Engineering School of São Carlos, University of São Paulo, Avenida Trabalhador São Carlense, 400, C.P. 292, São Carlos, SP, CEP 13560-970, Brazil. pgiloni@gmail.com
authors.forename: Patrícia C
authors.initials: PC
authors.lastname: Giloni-Lima
authors.forename: Danieli
authors.initials: D
authors.lastname: Delello
authors.forename: Marcelo L M
authors.initials: ML
authors.lastname: Cremonez
authors.forename: Márcia N
authors.initials: MN
authors.lastname: Eler
authors.forename: Vanderlei A
authors.initials: VA
authors.lastname: Lima
authors.forename: Evaldo L G
authors.initials: EL
authors.lastname: Espíndola
mesh_terms: D018533:Biomass; D002734:Chlorophyll / Q000378:metabolism; D000460:Chlorophyta / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002857:Chromium / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods; D012044:Regression Analysis; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D002857:Chromium; D002734:Chlorophyll