Trans-differentiation of iris pigmented epithelial cells of Euphlyctis cyanophlyctis tadpoles into lens in vitro.
Meshed pigmented iris epithelium along with neural retina of tadpoles of the frog Euphlyctis cyanophlyctis were found to undergo dedifferentiation and subsequently transdifferentiate into lens in culture medium. During lag period, depigmentation (dedifferentiation) occurred in many cells. When culture became confluent 3-4 weeks after seeding tiny lens like structures differentiated from foci of cultured pigmented iris epithelium cells. The percentage of lens formation was higher in vitamin A treated cases. The culture system appears to be a suitable for investigating the changes occurred during trans-differentiation of pigmented epithelial cells into lens.
Sharma
Manshi
M
Developmental Biology Laboratory, Department of Zoology, Dungar College, Bikaner 334 401, India.
Jangir
O P
OP
Jhajharia
Sunita
S
Singh
Sumer
S
Singh
Vijay
V
Nagal
Amit
A
D000818:Animals; D054338:Cell Transdifferentiation / Q000187:drug effects; D007498:Iris / Q000166:cytology* / Q000187:drug effects; D007814:Larva / Q000166:cytology / Q000254:growth & development; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000187:drug effects; D011898:Ranidae / Q000033:anatomy & histology / Q000254:growth & development*; D046509:Tissue Culture Techniques; D014801:Vitamin A / Q000494:pharmacology
D014801:Vitamin A