title: Efficient strategy for enhancing aspergiolide A production by citrate feedings and its effects on sexual development and growth of marine-derived fungus Aspergillus glaucus.
abstract: Aspergiolide A production enhancement by citrate and its effects on growth and sexual development of marine-derived fungus Aspergillus glaucus HB1-19 were investigated. In agar plate culture, 15 mM citric acid decreased colony radial growth and aspergiolide A production by 31.5% and 23.0%, respectively. It also improved sexual cleistothecium formation by 360% but depressed asexual conidiospore generation by 84.8%. In submerged culture, adding 40 mM citric acid finally promoted aspergiolide A production by 80.0%, which accompanied with 16.7% increase of biomass and 10.0% enhancement of sugar utilization. Differently, sodium citrate made no obvious or even opposite effects. Citrate and low pH could significantly improve pyruvate accumulation but inhibit succinate and fumarate production. Moreover, low pH was favorable to citrate utilization. Organic acids changes were closely related to aspergiolide A biosynthesis. Comparing to pH controls, effects of citric acid comprised pH decrease solicitation and citrate utilization enhancement.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Menghao
authors.initials: M
authors.lastname: Cai
authors.forename: Xiangshan
authors.initials: X
authors.lastname: Zhou
authors.forename: Jiushun
authors.initials: J
authors.lastname: Zhou
authors.forename: Chuanpeng
authors.initials: C
authors.lastname: Niu
authors.forename: Li
authors.initials: L
authors.lastname: Kang
authors.forename: Xueqian
authors.initials: X
authors.lastname: Sun
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000880:Anthraquinones / Q000378:metabolism*; D001230:Aspergillus / Q000187:drug effects / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D019343:Citric Acid / Q000008:administration & dosage*; D008386:Marine Biology; D009792:Oceans and Seas; D014871:Water Microbiology
chemical_list: D000880:Anthraquinones; C542894:aspergiolide A; D019343:Citric Acid