title: Porcine small intestinal epithelial cell line (IPEC-J2) of rotavirus infection as a new model for the study of innate immune responses to rotaviruses and probiotics.
abstract: Previous studies of epithelial immune responses to rotavirus infection have been conducted in transformed cell lines. In this study, we evaluated a non-transformed porcine jejunum epithelial cell line (IPEC-J2) as an in-vitro model of rotavirus infection and probiotic treatment. Cell-culture-adapted porcine rotavirus (PRV) OSU strain, or human rotavirus (HRV) Wa strain, along with Lactobacillus acidophilus (LA) or Lactobacillus rhamnosus GG (LGG) were used to inoculate IPEC-J2 cells. LA or LGG treatment was applied pre- or post-rotavirus infection. We demonstrated that IPEC-J2 cells were productively infected by PRV. LA or LGG treatment of the cells did not reduce virus replication. PRV infection increased MUC3 mucin secretion. LGG treatment post-rotavirus infection reduced the mucin secretion response induced by PRV; LGG alone increased the production of membrane-associated MUC3 mucin. LA treatment prior to rotavirus infection significantly increased PRV replication and the IL-6 response to PRV infection, which is consistent with the adjuvant effect of LA. LGG treatment post-rotavirus infection downregulated the IL-6 response, confirming the anti-inflammatory effect of LGG. IPEC-J2 cells expressed toll-like receptor (TLR) 2, TLR3, and TLR9 constitutively. TLR2 expression was upregulated by LGG and peptidoglycan, corresponding to the decreased IL-6 response, indicating that the protective effect of LGG is associated with upregulation of TLR2 expression on intestinal epithelial cells. The IPEC-J2 cell model of PRV infection is a completely homologous system. It is a valuable model for studying the interactions among rotavirus-host-probiotics, and the mechanisms behind the immunomodulating effect of probiotic bacteria on innate immune responses.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, P.R. China.
authors.forename: Fangning
authors.initials: F
authors.lastname: Liu
authors.forename: Guohua
authors.initials: G
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Wen
authors.forename: Tammy
authors.initials: T
authors.lastname: Bui
authors.forename: Dianjun
authors.initials: D
authors.lastname: Cao
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yuan
mesh_terms: D000818:Animals; D002460:Cell Line; D007113:Immunity, Innate; D015850:Interleukin-6 / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000276:immunology* / Q000821:virology; D055263:Mucin-3 / Q000378:metabolism; D019936:Probiotics; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D013552:Swine; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D014779:Virus Replication
chemical_list: D015850:Interleukin-6; D055263:Mucin-3; D051195:Toll-Like Receptor 2