Construction and comparison of Trametes versicolor laccase biosensors capable of detecting xenobiotics.
Amperometric biosensors using laccase from Trametes versicolor as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-D). Laccase enzyme was immobilized by gelatin and glutaraldehyde onto a Clark oxygen probe and screen printed electrodes (SPEs). Amperometric and chronoamperometric measurements were carried out with the biosensors. First, the effect of laccase activity on the biosensor performances was investigated for both biosensors, and then optimum pH and temperature and also thermal stability of the biosensors were tested. In addition, the detection ranges of some phenolic compounds were obtained by the help of calibration graphs of them. In repeatability studies, variation coefficients and standard deviations for both biosensors were also calculated by the studies done for this purposes. Finally, the biosensors were applied to the determination of 2,4-D in a real herbicide sample.
Sezgintürk
Mustafa Kemal
MK
Namik Kemal University, Faculty of Arts and Science, Chemistry Department, Tekirdağ, Turkey. msezginturk@hotmail.com
Odaci
Dilek
D
Pazarlioğlu
Nurdan
N
Pilloton
Roberto
R
Dinçkaya
Erhan
E
Telefoncu
Azmi
A
Timur
Suna
S
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D006540:Herbicides / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000378:metabolism*; D016014:Linear Models; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature; D055454:Trametes / Q000201:enzymology*; D014159:Transducers; D015262:Xenobiotics / Q000032:analysis*
D006540:Herbicides; D010636:Phenols; D015262:Xenobiotics; D015084:2,4-Dichlorophenoxyacetic Acid; D042845:Laccase