Live cell imaging of cytoskeletal dynamics in neurons using fluorescence photoactivation.
Alzheimer's disease and other neurodegenerative disorders share some common features at the cellular level, which are often associated with a change in cytoskeletal dynamics. Live cell imaging has been applied to study various aspects of cell physiology including cytoskeletal dynamics. Recently, fluorescence photoactivation (FPA) has been developed as a novel approach to analyze protein dynamics in living cells with high sensitivity. Here, we describe the application of FPA to determine interactions and mobility of the microtubule-associated protein tau in neurons as an example for a disease-related cytoskeletal protein and discuss further applications of FPA to study cytoskeletal dynamics in neurodegenerative diseases.
Gauthier
Anne
A
Department of Neurobiology, University of Osnabrück, Barbarastrasse 11, D-49076 Osnabrück, Germany.
Brandt
Roland
R
D000544:Alzheimer Disease / Q000378:metabolism; D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism*; D006801:Humans; D018613:Microscopy, Confocal; D019636:Neurodegenerative Diseases / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D016875:tau Proteins / Q000378:metabolism
D016875:tau Proteins