title: In vitro culture of Keratinocytes from human umbilical cord blood mesenchymal stem cells: the Saigonese culture.
abstract: There have been many attempts to acquire and culture human keratinocytes for clinical purposes including from keratotome slices in media with fetal calf serum (FCS) or pituitary extract (PE), from skin specimens in media with feeder layers, from suction blister epidermal roofs' in serum-free culture and from human umbilical cord blood (hUCB) mesenchymal stem cells (MSCs) in media with skin feeder layers. Conversely this study was designed to investigate whether keratinocytes could be obtained directly from hUCB MSCs in vitro. It is widely established that mesenchymal stem cells from human umbilical cord blood have multipotent capacity and the ability to differentiate into disparate cell lineages hUCB MSCs were directly induced to differentiate into keratinocytes by using a specific medium composed of primary culture medium (PCM) and serum free medium (SFM) in a ratio 1:9 for a period of 7 days and tested by immunostain p63 and K1-K10. Cells thus cultured were positive in both tests, confirming the possibility to directly obtain keratinocytes from MSCs hUCB in vitro.
authors.affiliation: Department of Histo-pathology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc Thach Medical University, Ho Chi Minh City, Vietnam. toaiphd@yahoo.com
authors.forename: Cong Toai
authors.initials: CT
authors.lastname: Tran
authors.forename: Duy Thao
authors.initials: DT
authors.lastname: Huynh
authors.forename: Ciro
authors.initials: C
authors.lastname: Gargiulo
authors.forename: Phuong Thao
authors.initials: PT
authors.lastname: Nguyen
authors.forename: Thi Thanh Thuy
authors.initials: TT
authors.lastname: Tran
authors.forename: Minh Tuan
authors.initials: MT
authors.lastname: Huynh
authors.forename: Thanh Tung
authors.initials: TT
authors.lastname: Nguyen
authors.forename: Luis
authors.initials: L
authors.lastname: Filgueira
authors.forename: D Micheal
authors.initials: DM
authors.lastname: Strong
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011247:Pregnancy