title: A haplotype in the CCR5 gene promoter was associated with the susceptibility to HIV-1 infection in a northern Chinese population.
abstract: It has been reported that single nucleotide polymorphisms (SNPs) in the promoter of the CCR5 gene are associated with the risk for HIV-1 infection and AIDS progression. Using resequencing, we performed a systematic survey of 78 HIV-1 seropositive individuals and 70 population-matched healthy control individuals from northern China to investigate SNPs of the CCR5 gene promoter and evaluated their effects on HIV-1 infection and the progression of AIDS. Linkage disequilibrium (LD) plots and haplotypes were generated using Haploview software. The association analyses were statistically compared using the Chi-square test with SPSS13.0 software for Windows. Seven SNPs (58755A>G, 58791C>T, 58934G>T, 59029A>G, 59353C>T, 59402A>G and 59653C>T) in the region of the CCR5 gene promoter were evaluated in this study. Among the seven SNPs, the minor allele frequencies of 58755G and 58791T were less than 2%. The differences in frequencies of the other five SNPs were not significant between case and control cohorts (P>0.05). In the case cohort, the association between these SNPs and clinical features (CD4+ T-lymphocyte counts and clinical categories) was not significant (P>0.05); however, there was a significant association between the haplotype GGTAC and susceptibility to HIV-1 infection (P<0.05), which is not consistent with other reports studied in different populations. The results suggest that the haplotype GGTAC may have a role in the process of HIV-1 infection in the northern Chinese population.
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Baojian Road 157, Nangang District, Harbin, 150081, China. xuld@ems.hrbmu.edu.cn
authors.forename: Lidan
authors.initials: L
authors.lastname: Xu
authors.forename: Yuandong
authors.initials: Y
authors.lastname: Qiao
authors.forename: Xuelong
authors.initials: X
authors.lastname: Zhang
authors.forename: Haiming
authors.initials: H
authors.lastname: Sun
authors.forename: Jingwei
authors.initials: J
authors.lastname: Wang
authors.forename: Donglin
authors.initials: D
authors.lastname: Sun
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Jia
authors.forename: Chao
authors.initials: C
authors.lastname: Shen
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Hong
authors.initials: H
authors.lastname: Ling
authors.forename: Kaili
authors.initials: K
authors.lastname: Wang
authors.forename: Songbin
authors.initials: S
authors.lastname: Fu
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D015331:Cohort Studies; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005828:Genetics, Population; D015658:HIV Infections / Q000235:genetics*; D015497:HIV-1 / Q000502:physiology*; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D019713:Receptors, CCR5 / Q000235:genetics*; D055815:Young Adult
chemical_list: D019713:Receptors, CCR5