title: Expressions of SE-1, CD31 and CD105 in the vascular endothelial cells and serum of rat with hepatocellular carcinoma.
abstract: BACKGROUND
  Hepatocellular carcinoma (HCC) is one of the deadliest cancers worldwide. In order to investigate the molecular biologic mechanism of HCC's development, we studied the expressions of SE-1, CD105 and CD31 in tumor endothelial cells (TECs) of HCC and in the serum of rats.
  
  
  METHODS
  We analyzed the expressions of SE-1, CD31 and CD105 in rat HCC tumor tissues using immunohistochemistry (IHC). Twenty HCC bearing rats and eighteen normal rats were examined for the expressions of SE-1, CD31 and CD105 antigens in serum by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  SE-1, CD31 and CD105 antigens were detected both in HCC tissue and in normal liver tissue with higher expressions of CD31 and CD105 in HCC while the SE-1 antigen expression was higher in normal liver. Similarly, serum CD31 and CD105 in rats with HCC were significantly increased compared with normal rats (t = 2.8628, P = 0.0086; t = 4.4922, P < 0.0001, respectively). In contrast, SE-1 antigen in HCC rat serum was significantly decreased compared with normal rats (t = 3.4983, P = 0.0011).
  
  
  CONCLUSIONS
  SE-1, CD31 and CD105 are closely related with liver tumor angiogenesis, which is similar to their performances in terms of their expressions in the serum.
authors.affiliation: Department of Infectious Disease, Peking University First Hospital, Beijing 100034, China.
authors.forename: Jing-yu
authors.initials: JY
authors.lastname: Wang
authors.forename: Xiao-yuan
authors.initials: XY
authors.lastname: Xu
authors.forename: Jing-hui
authors.initials: JH
authors.lastname: Jia
authors.forename: Chi-hong
authors.initials: CH
authors.lastname: Wu
authors.forename: Ruo-wen
authors.initials: RW
authors.lastname: Ge
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000097:blood*; D006528:Carcinoma, Hepatocellular / Q000098:blood supply / Q000737:chemistry*; D042783:Endothelial Cells / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D008114:Liver Neoplasms, Experimental / Q000098:blood supply / Q000737:chemistry*; D008297:Male; D009389:Neovascularization, Pathologic / Q000097:blood; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000097:blood*; D051381:Rats; D011915:Rats, Inbred BUF
chemical_list: D015703:Antigens, CD; D019408:Platelet Endothelial Cell Adhesion Molecule-1