title: Extraction of polysaccharide from Ganoderma lucidum and its immune enhancement activity.
abstract: In the present study, in order to maximize the yield of polysaccharides from Ganoderma lucidum, response surface methodology was employed to optimize the ultrasonic/microwave-assisted extraction (UMAE) conditions. The results indicated that the optimal extraction conditions were ultrasonic power of 50W, microwave power of 284W, extraction time of 701s and water/solid ratio of 11.6:1, respectively. Using UMAE, the yield of polysaccharides was 115.56% above that of classical hot water extraction (HWE) and increased by 27.7% as against ultrasound-assisted extraction (UAE), which confirmed the great potential application of UMAE technology in the extraction of polysaccharides. The immunological assays results demonstrated that polysaccharides of G. lucidum extracted by ultrasonic/microwave (UMP) could improve the weight of immune organ of immunocompromised mice, restore delayed-type hypersensitivity (DTH) reaction to DFNB, improve hemolysis antibody level and natural killer cell activity at high-dose. However, UMP had no noticeable effects on phagocytosis of monocyte at the tested dosage range.
authors.affiliation: South China University of Technology, Guangdong Province, Guangzhou, China.
authors.forename: Sheng-quan
authors.initials: SQ
authors.lastname: Huang
authors.forename: Zheng-xiang
authors.initials: ZX
authors.lastname: Ning
mesh_terms: D000818:Animals; D002244:Carbon / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D006460:Hemolysin Proteins / Q000097:blood; D006358:Hot Temperature; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007155:Immunologic Factors / Q000276:immunology* / Q000302:isolation & purification*; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D051379:Mice; D008872:Microwaves; D011134:Polysaccharides / Q000276:immunology* / Q000302:isolation & purification*; D020940:Reishi / Q000737:chemistry*; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014465:Ultrasonics; D014867:Water / Q000737:chemistry
chemical_list: D006460:Hemolysin Proteins; D007155:Immunologic Factors; D011134:Polysaccharides; D014867:Water; D002244:Carbon