Sensitive enzyme-linked immunosorbent assay and rapid one-step immunochromatographic strip for fumonisin B1 in grain-based food and feed samples.
BACKGROUND
Maize contaminated with mycotoxin fumonisin B1 poses a global threat to agricultural production. In this study, polyclonal antibodies (pAb) specific to fumonisin B1 were generated from rabbits immunised with fumonisin B1-keyhole limpet haemocyanin (KLH). These antibodies were used to establish a sensitive competitive direct enzyme-linked immunosorbent assay (cdELISA) and gold nanoparticle immunochromatographic strip for detecting fumonisin B1 levels in maize-based foods and feeds.


RESULTS
In cdELISA, fumonisins B1, B2 and B3 at concentrations of 0.42, 0.58 and 81.5 ng mL(-1) respectively caused 50% inhibition (IC(50)) of binding of fumonisin B1-horseradish peroxidase (HRP) to the antibodies. Effective on-site detection of fumonisin B1 was achieved by developing a rapid and sensitive pAb-based gold nanoparticle immunochromatographic strip. This strip had a detection limit of 5 ng mL(-1) for fumonisin B1 in maize-based samples. Additionally, the whole analytical process could be completed within 10 min. Close examination of 15 maize-based samples by cdELISA revealed that 11 were fumonisin-positive, with a mean concentration of 435 +/- 20.1 ng g(-1). These results correlated well with those obtained by immunochromatographic strip.


CONCLUSIONS
Both cdELISA and immunochromatographic strip methods established in this study are sensitive for rapid detection of fumonisins in agricultural commodities.
Shiu
Chang-Min
CM
Department of Immunology, Antai Tian-Sheng Memorial Hospital, Pingtung, Taiwan.
Wang
Jing-Jhih
JJ
Yu
Feng-Yih
FY
D000821:Animal Feed / Q000633:toxicity*; D000818:Animals; D000906:Antibodies / Q000032:analysis; D002845:Chromatography / Q000379:methods; D003429:Cross Reactions; D002523:Edible Grain / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D037341:Fumonisins / Q000032:analysis* / Q000276:immunology; D006046:Gold; D006433:Hemocyanins; D006735:Horseradish Peroxidase / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D053758:Nanoparticles; D011817:Rabbits; D003313:Zea mays / Q000737:chemistry*
D000906:Antibodies; D037341:Fumonisins; C056933:fumonisin B1; D006046:Gold; D006433:Hemocyanins; D006735:Horseradish Peroxidase; C032808:keyhole-limpet hemocyanin