title: Synergic effect of genotype changes in pertussis toxin and pertactin on adaptation to an acellular pertussis vaccine in the murine intranasal challenge model.
abstract: The Bordetella pertussis pertussis toxin and pertactin (Prn) are protective antigens and are contained in acellular pertussis vaccines. Polymorphisms in the A subunit of pertussis toxin (PtxA) and pertactin have been proposed to mediate vaccine resistance and contribute to pertussis reemergence. To test this hypothesis, previous studies compared clinical isolates expressing different alleles for the proteins. However, other virulence factors or virulence factor expression levels also may vary, confounding the analysis. To overcome these limitations, we constructed isogenic mutants of B. pertussis Tohama expressing the alleles ptxA1 or ptxA2 and prn1 or prn2 and compared the efficacies of an acellular pertussis vaccine against the mutants in a mouse model. While the vaccine was effective against all of the B. pertussis strains regardless of the allele expression pattern, the strain expressing ptxA1 and prn2 displayed a survival advantage over the other strains. These results suggest that an allele shift to the ptxA1 prn2 genotype may play a role in the emergence of pertussis in vaccinated populations.
authors.affiliation: Kitasato University, Research Center for Biologicals, 6-111 Arai, Kitamoto, Saitama, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Komatsu
authors.forename: Fuminori
authors.initials: F
authors.lastname: Yamaguchi
authors.forename: Akio
authors.initials: A
authors.lastname: Abe
authors.forename: Alison A
authors.initials: AA
authors.lastname: Weiss
authors.forename: Mineo
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001886:Bordetella pertussis / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D005260:Female; D005838:Genotype; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability; D037342:Pertussis Toxin / Q000235:genetics / Q000276:immunology*; D010567:Pertussis Vaccine / Q000235:genetics / Q000276:immunology*; D022282:Vaccines, Acellular / Q000235:genetics / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000235:genetics / Q000276:immunology*; D014917:Whooping Cough / Q000276:immunology / Q000517:prevention & control*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D010567:Pertussis Vaccine; D022282:Vaccines, Acellular; D010566:Virulence Factors, Bordetella; C068929:pertactin; D037342:Pertussis Toxin