title: Construction of uric acid biosensor based on biomimetic titanate nanotubes.
abstract: A uric acid biosensor has been fabricated through the immobilization of uricase on glassy carbon electrode modified by biomimetic titanate nanotubes of high specific surface area synthesized by hydrothermal decomposition. The so-constructed biosensor presents a high affinity to uric acid with a small apparent Michaelis-Menten constant of only 0.66 mM. The biosensor exhibits fairly good electrochemical properties such as the high sensitivity of 184.3 microAcm(-2)mM(-1), the fast response of less than 2 s, as well as the wide linear range from 1 microM to 5 mM. These performances indicate that titanate nanotubes could provide a favorable microenvironment for uricase immobilization, stabilize its biological activity, and function as an efficient electron conducting tunnel to facilitate the electron transfer. This suggests an important potential of titanate nanotubes in uric acid biosensors.
authors.affiliation: Key Laboratory of Mesoscopic Chemistry of MOE and Jiangsu Provincial Lab for Nanotechnology, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Haisheng
authors.initials: H
authors.lastname: Tao
authors.forename: Xuebin
authors.initials: X
authors.lastname: Wang
authors.forename: Xizhang
authors.initials: X
authors.lastname: Wang
authors.forename: Yemin
authors.initials: Y
authors.lastname: Hu
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Ma
authors.forename: Yinong
authors.initials: Y
authors.lastname: Lu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Hu
mesh_terms: D015374:Biosensing Techniques; D046529:Microscopy, Electron, Transmission; D018716:Molecular Mimicry; D043942:Nanotubes; D014025:Titanium / Q000737:chemistry*; D014527:Uric Acid / Q000032:analysis*
chemical_list: D014527:Uric Acid; D014025:Titanium