siRNA delivery using nanocarriers - an efficient tool for gene silencing.
Small interfering RNAs (siRNA) are one of the most recent additions used to silence gene expression. At present siRNA is the most extensively used gene-silencing technique over other nucleic-acid based approaches to treat disease including cancer, hepatitis, respiratory disease, cardiovascular diseases, neuronal disease and autoimmune disease. However, systemic delivery of siRNA in vivo, remains to be the biggest challenge to be overcome. Various strategies have been developed to deliver siRNA efficiently into target cell such as chemical modification of siRNA, physical strategies, viral and non viral-vectors mediated delivery. Among all the approaches non viral vectors including lipoplexes and polyplexes were found to be most successful which have been reviewed in this article. Further therapeutic applications of RNAi have also been briefly reviewed.
Khurana
Bharat
B
Nanomedicine Research Centre, Department of Pharmaceutics, Indo-Soviet Friendship, College of Pharmacy, Moga, Punjab, India.
Goyal
Amit K
AK
Budhiraja
Abhishek
A
Arora
Daisy
D
Vyas
Suresh P
SP
D000818:Animals; D016503:Drug Delivery Systems; D020868:Gene Silencing; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D053758:Nanoparticles; D004364:Pharmaceutical Preparations; D034741:RNA, Small Interfering / Q000008:administration & dosage*
D004364:Pharmaceutical Preparations; D034741:RNA, Small Interfering