title: Fractionation and evaluation of radical-scavenging peptides from in vitro digests of buckwheat protein.
abstract: Buckwheat protein (BWP) isolate was subjected to a two-stage in vitro digestion (1 h pepsin followed by 2 h pancreatin at 37 °C). The antioxidant potential of the BWP digests was compared by assessing their capacity to scavenge 2,2'-azinobis (3-ethylbenzothiszoline-6-sulphonic acid) (ABTS(+•)) and hydroxyl ((•)OH) radicals. The 2-h pancreatin digest, which demonstrated the strongest activity against both radicals, was subjected to Sephadex G-25 gel filtration. Of the six fractions collected, fractions IV (456 Da) and VI (362 Da) showed the highest ABTS(+•) scavenging activity and were 23-27% superior to mixed BWP digest (P < 0.05). Fraction VI was most effective in neutralizing (•)OH and was 86 and 24% more efficient (P < 0.05) than mixed BWP digest and fraction IV, respectively. LC-MS/MS identified Trp-Pro-Leu, Val-Pro-Trp, and Val-Phe-Pro-Trp (IV), Pro-Trp (V) and tryptophan (VI) to be the prominant peptides/amino acid in these fractions.
authors.affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ma
authors.forename: Youling L
authors.initials: YL
authors.lastname: Xiong
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhai
authors.forename: Haining
authors.initials: H
authors.lastname: Zhu
authors.forename: Thomas
authors.initials: T
authors.lastname: Dziubla