In vivo evaluation of (64)Cu-labeled magnetic nanoparticles as a dual-modality PET/MR imaging agent.
A novel nanoparticle-based dual-modality positron emission tomograph/magnetic resonance imaging (PET/MRI) contrast agent was developed. The probe consisted of a superparamagnetic iron oxide (SPIO) core coated with PEGylated phospholipids. The chelator 1,4,7,10-tetraazacyclo-dodecane-1,4,7,10-tetraacetic acid (DOTA) was conjugated to PEG termini to allow labeling with positron-emitting (64)Cu. Radiolabeling with (64)Cu at high yield and high purity was readily achieved. The (64)Cu-SPIO probes produced strong MR and PET signals and were stable in mouse serum for 24 h at 37 degrees C. Biodistribution and in vivo PET/CT imaging studies of the probes showed a circulation half-life of 143 min and high initial blood retention with moderate liver uptake, making them an attractive contrast agent for disease studies.
Glaus
Charles
C
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
Rossin
Raffaella
R
Welch
Michael J
MJ
Bao
Gang
G
D000818:Animals; D003287:Contrast Media / Q000737:chemistry; D003301:Copper Radioisotopes / Q000737:chemistry*; D005260:Female; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry; D007553:Isotope Labeling; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D049268:Positron-Emission Tomography; D013499:Surface Properties; D014018:Tissue Distribution
D003287:Contrast Media; D003301:Copper Radioisotopes; D006573:Heterocyclic Compounds, 1-Ring; D010743:Phospholipids; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; D011092:Polyethylene Glycols; D052203:Ferrosoferric Oxide