title: Contribution of SELP and PSGL-1 genotypes and haplotypes to the presence of coronary heart disease in Tunisians.
abstract: P-selectin (SELP) and its counter-receptor, P-selectin glycoprotein ligand-1 (PSGL-1), play key role in the transient attachment of leukocytes to endothelial cells predisposing to coronary heart disease (CHD). In the current report, 293 angiographically proven CHD patients and 327 age, gender, and race-matched controls were included. Our aim was to evaluate the contribution to CHD of the following SNPs: C-2123G, G-1969A and T715P in SELP, Met62Ile and the VNTR variants in PSGL-1 gene in a North African population from Tunisia. While there were no significant differences in the distribution of SELP or PSGL-1 alleles or genotypes between patients and controls, a trend for a significant association of the C-2123G genotypes distribution with incident CHD was observed (P=0.06). Assuming an additive model of transmission, the risk was 74% higher among subjects carrying the GG genotypes in comparison to those carrying the CC genotype (OR=1.74 [1.01-2.98], P=0.04) and 80% higher in the recessive model (OR=1.80 [1.08-3.01], P=0.02). Haplotype analysis did not identify any specific SELP or PSGL-1 haplotypes to be associated with CHD. The present study demonstrated no evidence of association between individual SELP or PSGL-1 SNPs or haplotypes with incident CHD. However, this study replicates absence of association of the mostly studied SNP, T715P, previously reported in individuals with African origin.
authors.affiliation: Research Unit of Biology and Genetics of Cancer, Haematological and Autoimmune Diseases, Monastir, 5000, Tunisia. ghazouani2005@yahoo.fr
authors.forename: Lakhdar
authors.initials: L
authors.lastname: Ghazouani
authors.forename: Nesrine
authors.initials: N
authors.lastname: Abboud
authors.forename: Sonia Ben Hadj
authors.initials: SB
authors.lastname: Khalifa
authors.forename: Claire
authors.initials: C
authors.lastname: Perret
authors.forename: Viviane
authors.initials: V
authors.lastname: Nicaud
authors.forename: Wassim Youssef
authors.initials: WY
authors.lastname: Almawi
authors.forename: François
authors.initials: F
authors.lastname: Cambien
authors.forename: Touhami
authors.initials: T
authors.lastname: Mahjoub
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D003327:Coronary Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D016017:Odds Ratio; D019007:P-Selectin / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D014416:Tunisia
chemical_list: D008562:Membrane Glycoproteins; D019007:P-Selectin; C084882:P-selectin ligand protein; C546937:SELP protein, human