title: [Characteristics of CDR3 of TCR beta on CD8+ T cells in chronic hepatitis B patients].
abstract: OBJECTIVE
  To analyze the characteristics of CDR3 of TCRbeta on CD8+ T cells in chronic hepatitis B patients.
  
  
  METHODS
  Eight patients with chronic hepatitis B (ALT more than 2 ULN) were enrolled in this study. CD8+ T cells were isolated from peripheral blood. RT-PCR was proformed to amplify the CDR3 of TCRbeta, and the PCR products were sequenced and analyzed.
  
  
  RESULTS
  The chronic hepatitis B patients showed obvious clonal expansion of T cell, and three perturbation patterns of T cell expansion were showed in the CDR3 of TCRbeta, including monoclonicity, oligoclonicity and skewed peak patterns. The number of perturbation families of CD8+ subpopulation was significantly higher than that of CD8- subpopulation (10.6+/-4.7 vs. 4.1+/-3.1, t = 6.619, P less than 0.01). In 3 out of 8 patients, the number of perturbation families of CD8+ subpopulation was also higher than that of PBMCs without depleting CD8+ subpopulation.
  
  
  CONCLUSIONS
  The characteristics of CDR3 of TCRbeta may help to understand the inflammatory response in CHB patients.
authors.affiliation: Department of Infectious Disease, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yong-Yin
authors.initials: YY
authors.lastname: Li
authors.forename: Shi-Wu
authors.initials: SW
authors.lastname: Ma
authors.forename: Guang-Wen
authors.initials: GW
authors.lastname: Zhang
authors.forename: Xuan
authors.initials: X
authors.lastname: Huang
authors.forename: Xiao-Xiong
authors.initials: XX
authors.lastname: Hu
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.forename: Jin-Lin
authors.initials: JL
authors.lastname: Hou
mesh_terms: D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D022801:Complementarity Determining Regions / Q000235:genetics*; D019674:Genes, T-Cell Receptor beta; D019694:Hepatitis B, Chronic / Q000097:blood* / Q000276:immunology*; D006801:Humans; D008297:Male; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D055815:Young Adult
chemical_list: D022801:Complementarity Determining Regions; D011948:Receptors, Antigen, T-Cell