title: Effects of low-level light therapy on streptozotocin-induced diabetic kidney.
abstract: Hyperglycemia causes oxidative damage in tissues prone to complications in diabetes. Low-level light therapy (LLLT) in the red to near infrared range (630-1000nm) has been shown to accelerate diabetic wound healing. To test the hypothesis that LLLT would attenuate oxidative renal damage in Type I diabetic rats, male Wistar rats were made diabetic with streptozotocin (50mg/kg, ip), and then exposed to 670nm light at a dose of 9J/cm(2) once per day for 14weeks. The activity and expression of catalase and the activity of Na K-ATPase increased in kidneys of light-treated diabetic rats, whereas the activity and expression of glutathione peroxidase and the expression of Na K-ATPase were unchanged. LLLT lowered the values of serum BUN, serum creatinine, and BUN/creatinine ratio. In addition, LLLT augmented the activity and expression of cytochrome c oxidase, a primary photoacceptor molecule in the mitochondrial respiratory chain, and reduced the formation of the DNA adduct 8-hydroxy-2'-deoxyguanosine in kidney. LLLT improved renal function and antioxidant defense capabilities in the kidney of Type I diabetic rats. Thus, 670nm LLLT may be broadly applicable to the amelioration of renal complications induced by diabetes that disrupt antioxidant defense mechanisms.
authors.affiliation: School of Public and Environmental Affairs, Indiana University, 1315 East Tenth Street, Bloomington, IN 47405, United States.
authors.forename: Jinhwan
authors.initials: J
authors.lastname: Lim
authors.forename: Ruth A
authors.initials: RA
authors.lastname: Sanders
authors.forename: Ann C
authors.initials: AC
authors.lastname: Snyder
authors.forename: Janis T
authors.initials: JT
authors.lastname: Eells
authors.forename: Diane S
authors.initials: DS
authors.lastname: Henshel
authors.forename: John B
authors.initials: JB
authors.lastname: Watkins
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D002374:Catalase / Q000378:metabolism; D003401:Creatine / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000201:enzymology / Q000628:therapy*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007259:Infrared Rays; D007668:Kidney / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D008297:Male; D010789:Phototherapy; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D002374:Catalase; D005979:Glutathione Peroxidase; D000254:Sodium-Potassium-Exchanging ATPase; D003401:Creatine