Study on polymerization effect of polyembryony genes by SSCP marker and family trees in Chinese goats.
The aim of experiment was to analyze the polymerization effect of genotypes and genotype combinations of PRLR and LHβ gene loci in Xinong Saanen goat by SSCP marker and family trees. The relationships of genotype combinations and litter size were compared in Xinong Saanen goat. The results indicate that there are genetic polymorphisms at the PRLR and LHβ gene loci, and there are 4 positive genotypes (GG, CC, PP and LL) and 4 negative genotypes (HH, DD, QQ and MM) effects on litter size, respectively in Xinong Saanen goat. Compared with the other genotype combinations, the polymerization effect of GGCCPPLL markedly improved (P < 0.05). The polymerization effect value of CC genotype was higher than that of CD genotype by 14.12%, and MM genotype was higher than that of LM genotype by 3.80%, and PP genotype was higher than that of QQ genotype by 15.67%, and LL genotype was higher than that of LM genotype by 11.48%, and PQ genotype was higher than that of QQ genotype by 11.02%, and CD genotype was higher than that of DD genotype by 10.69%, and PQ genotype was higher than that of PP genotype by 6.09%. There was significantly polymerization effect in the course of reproduction from parental generation (F0) to F1 generation. However, there was significantly gene isolation effect in the course of reproduction from F1 generation to F2 generation. This result can be used to guide the goat breeding in polyembryony trait.
Li
Guang
G
College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, 712100, Yangling, Shaanxi, People's Republic of China.
An
Xiaopeng
X
Hou
Jinxing
J
Li
Ling
L
Han
Dan
D
Yang
Mingming
M
Wang
Yana
Y
Zhu
Guangqin
G
Wang
Jiangang
J
Song
Yuxuan
Y
Cao
Bin Yun
BY
D000818:Animals; D002681:China; D005838:Genotype; D006041:Goats / Q000235:genetics*; D008098:Litter Size; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011981:Receptors, Prolactin / Q000235:genetics; D017422:Sequence Analysis, DNA
D037101:Luteinizing Hormone, beta Subunit; D011981:Receptors, Prolactin