title: Biocompatible graphene oxide-based glucose biosensors.
abstract: This letter demonstrates that a novel, highly efficient enzyme electrode can be directly obtained using covalent attachment between carboxyl acid groups of graphene oxide sheets and amines of glucose oxidase. The resulting biosensor exhibits a broad linear range up to 28 mM x mm(-2) glucose with a sensitivity of 8.045 mA x cm(-2) x M(-1). The glucose oxidase-immobilized graphene oxide electrode also shows a reproducibility and a good storage stability, suggesting potentials for a wide range of practical applications. The biocompatibility of as-synthesized graphene oxide nanosheets with human cells, especially retinal pigment epithelium (RPE) cells, was investigated for the first time in the present work. Microporous graphene oxide exhibits good biocompatibility and has potential advantages with respect to cell attachment and proliferation, leading to opportunities for using graphene-based biosensors for the clinical diagnosis.
authors.affiliation: Biomedical Engineering Academy, School of Ophthalmology & Optometry, Wenzhou Medical College, 270 Xueyuan Road, Wenzhou 325027, China. yongliu1980@hotmail.com
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Dingshan
authors.initials: D
authors.lastname: Yu
authors.forename: Chao
authors.initials: C
authors.lastname: Zeng
authors.forename: Zongcheng
authors.initials: Z
authors.lastname: Miao
authors.forename: Liming
authors.initials: L
authors.lastname: Dai
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence
chemical_list: D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose