[Advances in microbial production of alkaline polygalacturonate lyase and its application in clean production of textile industry].
We reviewed the microbial production of alkaline polygalacturonate lyase (PGL) and its application in the clean production of textile industry. Currently PGL is mainly produced by microbial fermentation and Bacillus sp. is an ideal wild strain for PGL production. Microbial PGL production was affected by many factors including the concentration and feeding mode of substrate, cell concentration, agitation speed, aeration rate, pH and temperature. Constructing the recombinant strain provided an effective alternative for PGL production, and the concentration of PGL produced by the recombinant Pichia pastoris reached 1305 U/mL in 10 m3 fermentor. The recombinant Pichia pastoris had the potential to reach the industrial production of PGL. PGL can be applied in bio-scouring process in the pre-treatment of cotton. Compared with the traditional alkaline cooking process, the application of PGL can protect fiber, improve the bio-scouring efficiency, decrease energy consumption and alleviate the environmental pollution. The future research focus will be the molecular directed evolution of PGL to make PGL more suitable for the application of PGL in bio-scouring process to realize the clean production of textile industry.
Liu
Long
L
School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Wang
Zhihao
Z
Zhang
Dongxu
D
Li
Jianghua
J
Du
Guocheng
G
Chen
Jian
J
D000468:Alkalies; D001412:Bacillus subtilis / Q000378:metabolism; D004787:Environmental Pollution / Q000517:prevention & control; D005285:Fermentation; D007218:Industrial Microbiology; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013783:Textile Industry
D000468:Alkalies; D011994:Recombinant Proteins; D011133:Polysaccharide-Lyases; C023911:pectate lyase