title: Revealing the lytic mechanism of the antimicrobial peptide gomesin by observing giant unilamellar vesicles.
abstract: Gomesin (Gm) is a potent cationic antimicrobial peptide from a Brazilian spider. Here we use optical and fluorescence microscopy to study the interaction of Gm, its low active linear analogue, [Ser(2,6,11,15)]-Gm (GmL), and a fluorescent labeled analogue, Gm-Rh, with giant unilamellar vesicles (GUVs) composed of mixtures of the neutral lipid palmitoyloleoyl phosphatidylcholine (POPC) with the negatively charged lipid palmitoyloleoyl phosphatidylglycerol (POPG) or cholesterol, so as to mimic bacterial and mammalian cell membranes, respectively. We observed the effect of injecting a peptide solution with a micropipet close to GUVs. As a result of peptide-lipid interaction, GUVs burst suddenly. Stable pores, which result in leaky vesicles, were not observed. Fluorescence microscopy of Gm-Rh injected on GUVs confirmed the high peptide/lipid affinity. These facts lead us to suggest that Gm and GmL disrupt the membrane via the carpet model. In order to quantify the lytic activity of both peptides against different membrane composition, a solution of GUVs was diluted in increasing concentration of peptides and the fraction of burst GUVs was measured as a function of time. The lytic activity of both peptides was enhanced by the presence of POPG and decreased upon addition of cholesterol. GmL exhibited lower lytic activity as compared to Gm, but this difference vanished at high POPG molar fraction.
authors.affiliation: Departamento de Biofísica, Universidade Federal de São Paulo, 100 CEP 04044-020, São Paulo, SP, Brazil.
authors.forename: Tatiana M
authors.initials: TM
authors.lastname: Domingues
authors.forename: Karin A
authors.initials: KA
authors.lastname: Riske
authors.forename: Antonio
authors.initials: A
authors.lastname: Miranda
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D008962:Models, Theoretical; D010713:Phosphatidylcholines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D023181:Antimicrobial Cationic Peptides; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D053835:Unilamellar Liposomes; C415987:gomesin; C060037:1-palmitoyl-2-oleoylglycero-3-phosphoglycerol; C028694:1-palmitoyl-2-oleoylphosphatidylcholine