Universal indirect enzyme-linked immunosorbent assay for monitoring of human and animal brucellosis in Kazakhstan.
Combinations of conventional serological methods and new ELISA procedures were evaluated to develop the most efficient and effective diagnostics for the detection of brucellosis in humans and animals. Sera from humans (n=249) and animals (n=99) were collected from brucellosis endemic areas (Zhambyl district and Enbekshi-Kazakh district of Almaty region in Kazakhstan) for serologic analysis. Sera from the humans reacted positively in the RBT (38.5%), SAT (43.3%), iELISA (42.5%) while sera from the animals reacted positively in RBT (79.8%), SAT (89.9%), CF (87.8%), iELISA (100%). Greater seropositivity was detected in animals as compared to human samples. All positive sera were also evaluated on an indirect ELISA (iELISA). Bacterial isolation was attempted on seropositive human sera. Our data indicate that the combination of conventional serological tests (SAT and CF), combined with the iELISA is optimal for the processing of large numbers of samples and the most efficient detection of human and animal brucellosis.
Grushina
T
T
M. Aikimbayev's Kazakh Scientific Center for Quarantine and Zoonotic Diseases, Kazakhstan. grushin@caidc.katelco.com
Atshabar
B
B
Syzdykov
M
M
Daulbaeva
S
S
Tserelson
L
L
Kuznetsov
A
A
Baramova
Sh
Sh
Seidakhmetova
R
R
Sultanov
A
A
Ospanov
Y
Y
Mikhalev
A
A
Amireev
S
S
Ospanov
K
K
Kazakov
S
S
Mizanbayeva
S
S
Myrzabekov
A
A
Rementsova
M
M
Berezovskiy
D
D
Akasheva
R
R
Khasenov
M
M
Nussipova
Zh
Zh
Yu
W
W
Nielsen
K
K
D000372:Agglutination Tests / Q000379:methods; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002006:Brucellosis / Q000175:diagnosis* / Q000276:immunology; D003168:Complement Fixation Tests / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007623:Kazakhstan
D000907:Antibodies, Bacterial