Recognition and modulation of Dectin-1 and TLR-2 receptors by curdlan derivatives and purified natural extracts.
Toll-like receptors (TLRs) and Dectin-1, as members of Pattern Recognition Receptors play an essential role in innate immune response against bacteria and fungi respectively, contributing to pathogens recognition, phagocytosis, etc. Dectin-1 and TLR-2/TLR-6 can interact for intracellular signal transduction. Dectin-1 is expressed at low levels on macrophages and at high levels on dendritic cells. Dectin-1 and TLRs are synergistic in mediating cytokines production, such as IL-12 and tumor necrosis factor alpha (TNF alpha). In the present paper we studied the expression of Dectin-1 (beta-Glucan Receptor C-type lectin receptor class V) and TLR-2 on human normal monocytes cells and also the role of different Curdlan derivatives and highly purified natural extracts, especially their capacity to recognize these receptors and their Dectin-1 agonist/antagonist properties. Our results demonstrated that Curdlan derivatives containing sulfopropyl or palmythoil/carboximethyl/sulfopropyl groups and natural extracts could be potent immunomodulators with many potential applications (possible antagonists of Dectin-1, blockers of Dectin-1 cooperation with TLR-2).
Călugăru
Ana
A
National Institute for Research/Development in Microbiology and Immunology Cantacuzino, Immunomodulation Department, Bucharest, Romania. sni@cantacuzino.ro
Cremer
Lidia
L
Lupu
Andreea Roxana
AR
Bădulescu
Mihaela Maria
MM
Apetrei
Natalia Simona
NS
Moscovici
M
M
Mocanu
Georgeta
G
Mihai
Doina
D
Kerek
F
F
Szegli
G
G
D005434:Flow Cytometry; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D037181:Lectins, C-Type; D008565:Membrane Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000276:immunology*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000276:immunology*; D047071:beta-Glucans / Q000276:immunology / Q000494:pharmacology*; D000099181:Dectin-1
D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D051195:Toll-Like Receptor 2; D047071:beta-Glucans; D000099181:Dectin-1; C495342:TLR2 protein, human; C038459:curdlan