title: Investigation of Panton-Valentine leukocidin expressing Staphylococcus aureus colonization among children in a child care center.
abstract: The presence of Panton-Valentine leukocidin expressing Staphylococcus aureus colonization was investigated with a qualitative nucleic acid hybridization assay among 122 children and 19 staff in a child care center. Genotyping of 5 Panton-Valentine leukocidin-positive isolates by pulsed-field gel electrophoresis revealed that one child and a teacher from the same class were colonized with the clonally related strains. This finding allowed us to suggest that close contact with colonized people is a risk factor for being colonized.
authors.affiliation: Süleyman Demirel University Faculty of Medicine, Medical Microbiology Department, Isparta, Turkey. seslicetin@med.sdu.edu.tr
authors.forename: Emel
authors.initials: E
authors.lastname: Sesli Cetin
authors.forename: Ebru
authors.initials: E
authors.lastname: Us
authors.forename: Hayati
authors.initials: H
authors.lastname: Güneş
authors.forename: Selçuk
authors.initials: S
authors.lastname: Kaya
authors.forename: Alper
authors.initials: A
authors.lastname: Tekeli
authors.forename: Mustafa
authors.initials: M
authors.lastname: Demirci
mesh_terms: D000328:Adult; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques / Q000379:methods; D002648:Child; D002656:Child Day Care Centers; D002675:Child, Preschool; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005098:Exotoxins / Q000096:biosynthesis* / Q000235:genetics; D005838:Genotype; D006801:Humans; D007223:Infant; D007956:Leukocidins / Q000096:biosynthesis* / Q000235:genetics; D017720:Molecular Epidemiology / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D014481:United States; D037521:Virulence Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D037521:Virulence Factors