[Neuroprotective effect of extract of Ginkgo biloba against excitotoxicty compared with ginkgolide B in neuron cell of rat].
OBJECTIVE
To observe the effect of the extract of Ginkgo biloba (EGb761) and the components isolated from the extract named ginkgolide B (GB) against damage of glutamate in pretreatment modes so that determine their application value and approach.


METHODS
Based on glutamate-induced excitotoxicity to primary cultures from neonatal Sprague-Dawley (SD) rat hippocampal neuron, our experiment utilized trypan blue, TUNEL and LDH to study the effect of EGb761 and GB on neuron in different doses pretreatment modes, as well as to compare with the NMDA receptor uncompetitive antagonist-MK-801.


RESULTS
EGb761 and GB can recrease cell viability, reduce apoptosis rate and decrease LDH leakage in different degree and depended on dose in certain range. The maximal protection was achieved at a concentration of 100 mg x L(-1), 100 micromol x L(-1), but inferior to MK-801 (10 micromol x L(-1)). The protective effect of GB is superior to EGb761.


CONCLUSIONS
Treatment with EGb761 and GB could protect the neurons against glutamate-induced injury. The maximal protection of GB was achieved by pretreatment is superior to EGb761, so its precautionanary intervention to high-risk population could have more value.
Xu
Jing
J
Functional laboratory, Dalian Medical University, Dalian 116044, Chiha.
Sun
Changkai
C
Ma
Hui
H
Wang
Lu
L
Zhang
Jian
J
Zhang
Yumei
Y
Wu
Lanxiang
L
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry*; D046934:Ginkgolides / Q000737:chemistry / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007783:Lactones / Q000737:chemistry / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000096983:Ginkgo Extract
D016291:Dizocilpine Maleate; D004365:Drugs, Chinese Herbal; D046934:Ginkgolides; D007770:L-Lactate Dehydrogenase; D007783:Lactones; D018696:Neuroprotective Agents; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract; C045856:ginkgolide B