DistanceScan: a tool for promoter modeling.
CONCLUSIONS
The state of the art in promoter modeling for higher eukaryotes is predicting not single transcription factor binding sites (TFBSs), but their combinations. The new tool utilizes a previously developed method of distance distributions of TFBS pairs. We model the random distribution of distances and compare it with the distribution observed in the query sequences. Comparison of the profiles allows filtering out the 'noise' and retaining the potentially functional combinations. This approach has proved its usefulness as a filtering technique for the selection of TFBS pairs for promoter modeling and is now implemented as a tool in R. As an input, it can use the outputs of three different TFBS- and motif-predictive tools (Gibbs Sampler for motifs, Match and MEME/FIMO for PWM-based search). The output is a list of predicted pairs on overrepresented distances with assigned scores, P-values and plots showing the distribution of pairs in the input sequences.


BACKGROUND
The tool is available at https://www.omnifung.hki-jena.de/Rpad/Distance_Scan/index.htm.
Shelest
Vladimir
V
Research group Systems Biology/Bioinformatics, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Beutenbergstr. 11a, 07745 Jena, Germany.
Albrecht
Daniela
D
Shelest
Ekaterina
E
D001665:Binding Sites; D030541:Databases, Genetic; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods; D012984:Software; D014157:Transcription Factors / Q000378:metabolism
D014157:Transcription Factors