title: Broome virus, a new fusogenic Orthoreovirus species isolated from an Australian fruit bat.
abstract: This report describes the discovery and characterization of a new fusogenic orthoreovirus, Broome virus (BroV), isolated from a little red flying-fox (Pteropus scapulatus). The BroV genome consists of 10 dsRNA segments, each having a 3' terminal pentanucleotide sequence conserved amongst all members of the genus Orthoreovirus, and a unique 5' terminal pentanucleotide sequence. The smallest genome segment is bicistronic and encodes two small nonstructural proteins, one of which is a novel fusion associated small transmembrane (FAST) protein responsible for syncytium formation, but no cell attachment protein. The low amino acid sequence identity between BroV proteins and those of other orthoreoviruses (13-50%), combined with phylogenetic analyses of structural and nonstructural proteins provide evidence to support the classification of BroV in a new sixth species group within the genus Orthoreovirus.
authors.affiliation: CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia.
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Thalmann
authors.forename: David Michael
authors.initials: DM
authors.lastname: Cummins
authors.forename: Meng
authors.initials: M
authors.lastname: Yu
authors.forename: Ross
authors.initials: R
authors.lastname: Lunt
authors.forename: Lindsay Ian
authors.initials: LI
authors.lastname: Pritchard
authors.forename: Eric
authors.initials: E
authors.lastname: Hansson
authors.forename: Sandra
authors.initials: S
authors.lastname: Crameri
authors.forename: Alex
authors.initials: A
authors.lastname: Hyatt
authors.forename: Lin-Fa
authors.initials: LF
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001315:Australia; D002460:Cell Line; D002685:Chiroptera / Q000821:virology*; D016000:Cluster Analysis; D006224:Cricetinae; D016679:Genome, Viral; D008969:Molecular Sequence Data; D018165:Orthoreovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012088:Reoviridae Infections / Q000662:veterinary*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins