title: Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells.
abstract: Phosphatidylinositol 3-phosphate (PtdIns(3)P) is a phospholipid residing on early endosomes, where it is proposed to be involved in endosomal fusion. We synthesized membrane-permeant derivatives of PtdIns(3)P, including a caged version that is to our knowledge the first photoactivatable phosphoinositide derivative developed so far. In living cells, photoactivation of caged PtdIns(3)P induced rapid endosomal fusion in an EEA1-dependent fashion, thus providing in vivo evidence that PtdIns(3)P is a sufficient signal for driving this process.
authors.affiliation: European Molecular Biology Institute, Cell Biology and Biophysics Unit, Heidelberg, Germany.
authors.forename: Devaraj
authors.initials: D
authors.lastname: Subramanian
authors.forename: Vibor
authors.initials: V
authors.lastname: Laketa
authors.forename: Rainer
authors.initials: R
authors.lastname: Müller
authors.forename: Christian
authors.initials: C
authors.lastname: Tischer
authors.forename: Sirus
authors.initials: S
authors.lastname: Zarbakhsh
authors.forename: Rainer
authors.initials: R
authors.lastname: Pepperkok
authors.forename: Carsten
authors.initials: C
authors.lastname: Schultz
mesh_terms: D002459:Cell Fusion; D002463:Cell Membrane Permeability; D011992:Endosomes / Q000648:ultrastructure*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*
chemical_list: D018129:Phosphatidylinositol Phosphates; C055525:phosphatidylinositol 3-phosphate