title: [Analysis of 16S rDNA sequence of Cronobacter spp].
abstract: OBJECTIVE
  To analyse the sequence of 16S rDNA of twenty-nine Cronobacter spp. strains isolated from food as well as two type strains.
  
  
  METHODS
  Using polymerase chain reaction to obtain the sequences of 16S rDNA of all strains. The multiple cluster analysis and phylogenetic analysis of them were studied by BioNumerics software.
  
  
  RESULTS
  All strains, except ATCC51329 and ES 014, were clustered with the Cronobacter spp. type strain ATCC29544. The Cronobacter spp. type strain ATCC51329 and ES 014 belonged to a different branch. From the sequence analysis of 16S rDNA, all these isolates of Cronobacter spp. except ES 014 could be identified accurately by gene analysis.
  
  
  CONCLUSIONS
  All Cronobacter spp. strains isolated from food were identified on genetic level. The phylogenetic relationships among related Cronobacter spp. isolates were studied.
authors.affiliation: Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China. pxycdc@sohu.com
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Pei
authors.forename: Xiumei
authors.initials: X
authors.lastname: Liu
mesh_terms: D044083:Cronobacter sakazakii / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis*; D005516:Food Microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S