title: Effects of scaling and root planing and subantimicrobial dose doxycycline on gingival crevicular fluid levels of matrix metalloproteinase-8, -13 and serum levels of HsCRP in patients with chronic periodontitis.
abstract: BACKGROUND
  This study evaluates the efficacy of subantimicrobial dose doxycycline (SDD) in conjunction with scaling and root planing (SRP) on gingival crevicular fluid (GCF) levels of matrix metalloproteinase (MMP)-8 and -13 and on serum levels of high-sensitivity C-reactive protein (HsCRP) in patients with chronic periodontitis (CP).
  
  
  METHODS
  A total of 41 patients with CP and 17 healthy individuals were included in this randomized controlled trial. CP patients were randomly distributed into two groups. Study groups were established as Group I with SRP+placebo, Group II with SRP+SDD, and Group III as control. All CP patients received two regimens of SRP and Group II patients also received SDD for 6 weeks. At baseline and 6 weeks, GCF and blood were collected and clinical indices were recorded. The HsCRP level was assayed in the plasma on a nephelometer. The GCF levels of MMPs were assayed by an enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Statistically significant differences in plaque index, gingival index (GI), probing depth (PD), GCF volumes, GCF MMP levels, and serum levels of HsCRP between pre-treatment and post-treatment were noted in both groups. Between groups there was a statistically significant decrease in PD, GI, and GCF levels of MMP-8 favoring the group receiving SDD adjunctive to SRP (P <0.05).
  
  
  CONCLUSIONS
  In this study, greater improvement was detected for PD, GI, and GCF levels of MMP-8 when using SRP+SDD compared to SRP+placebo.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Turkey. gulay@gazi.edu.tr
authors.forename: Gülay
authors.initials: G
authors.lastname: Tüter
authors.forename: Muhittin
authors.initials: M
authors.lastname: Serdar
authors.forename: Bülent
authors.initials: B
authors.lastname: Kurtiş
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Walker
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Atak
authors.forename: Utku
authors.initials: U
authors.lastname: Toyman
authors.forename: Selin
authors.initials: S
authors.lastname: Pinar
authors.forename: Tuba
authors.initials: T
authors.lastname: Aykan
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D002097:C-Reactive Protein / Q000032:analysis* / Q000187:drug effects; D055113:Chronic Periodontitis / Q000097:blood / Q000201:enzymology / Q000628:therapy*; D003774:Dental Plaque Index; D012534:Dental Scaling; D004318:Doxycycline / Q000008:administration & dosage*; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000032:analysis* / Q000187:drug effects; D020784:Matrix Metalloproteinase 8 / Q000032:analysis* / Q000187:drug effects; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000628:therapy; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000628:therapy; D010919:Placebos; D016745:Root Planing; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D010919:Placebos; D002097:C-Reactive Protein; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8; D004318:Doxycycline