Something worth dyeing for: molecular tools for the dissection of lipid metabolism in Caenorhabditis elegans.
The nematode Caenorhabditis elegans (C. elegans) has during the last decade emerged as an invaluable eukaryotic model organism to understand the metabolic and neuro-endocrine regulation of lipid accumulation. The fundamental pathways of food intake, digestion, metabolism, and signalling are evolutionary conserved between mammals and worms making C. elegans a genetically and metabolically extremely tractable model to decipher new regulatory mechanisms of lipid storage and to understand how nutritional and genetic perturbations can lead to obesity and other metabolic diseases. Besides providing an overview of the most important regulatory mechanisms of lipid accumulation in C. elegans, we also critically assess the current methodologies to monitor lipid storage and content as various methods differ in their applicability, consistency, and simplicity.
Elle
Ida Coordt
IC
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.
Olsen
Louise Cathrine Braun
LC
Pultz
Dennis
D
Rødkaer
Steven Vestergaard
SV
Faergeman
Nils Joakim
NJ
D000818:Animals; D001692:Biological Transport; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D050356:Lipid Metabolism; D015398:Signal Transduction
D005456:Fluorescent Dyes