Intratumoral administration of anti-KITENIN shRNA-loaded PEI-alt-PEG nanoparticles suppressed colon carcinoma established subcutaneously in mice.
Biodegradable gene carrier, termed as PEI-alt-PEG, has been synthesized based on Michael addition reaction between lower Mw PEI and poly(ethylene glycol) (PEG) diacrylate and tested its potential of anti-metastatic cancer gene therapy by using anti-KITENIN short hairpin RNA. KITENIN is known to promote invasion of mouse colon adenocarcinoma in vivo. Intratumoral administration of anti-KITENIN shRNA-loaded PEI-alt-PEG nanoparticles has shown suppressed proliferlation and enhanced apoptosis signal in tumor compared to commercial available liposome, leading to delayed tumor growth.
Park
In-Kyu
IK
Department of Biomedical Sciences, Chonnam National University Medical School, The Brain Korea 21 Project, Centre for Biomedical Human Resources at Chonnam National University, Gwangju 501-746, South Korea.
Kim
Kyung Keun
KK
Cho
Sang-Hee
SH
Bae
Woo-Kyun
WK
Jere
Dhananjay
D
Cho
Chong-Su
CS
Chung
Ik-Joo
IJ
D000230:Adenocarcinoma / Q000235:genetics* / Q000628:therapy*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D020868:Gene Silencing; D015316:Genetic Therapy / Q000379:methods*; D007279:Injections, Subcutaneous; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008968:Molecular Conformation; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D050997:Nanomedicine / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000031:analogs & derivatives / Q000737:chemistry; D012313:RNA / Q000008:administration & dosage* / Q000235:genetics; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014162:Transfection / Q000379:methods
D002352:Carrier Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D053769:Nanocapsules; C488287:VANGL1 protein, human; C544016:poly(ethylene glycol)-co-poly(ethyleneimine); C009495:titanium dioxide; D011092:Polyethylene Glycols; D012313:RNA; D011094:Polyethyleneimine; D014025:Titanium