[Effect of angelica polysaccharides co-erythropoietin on JAK2/STAT5 signal transduction pathway in hematopoietic stem/progenitor cells].
OBJECTIVE
To investgate the signal transduction and regulation in erythropoiesis by angelica polysaccharides (APS) to clarify the mechanism for APS promoting hematopoiesis.


METHODS
The mononuclear cells were isolated from foetus umbilic cord blood (mononuclear cells, MNCs), after MNCs were incubated in the presence of APS group (APS 200 mg x L(-1)) and control group for 24 h, the cells were stimulated with Epo (5 U x mL(-1)) for 0, 2, 5, 30 min, respectively. STAT5 was measured by ICC and laser confocal scanning microscope. JAK2, STAT5 in nucleus and cytoplasm were measured by western-blotting-ECL.


RESULTS
Angelica polysaccharide cooperated with Epo has significant impact on the expression of STAT5. The expression of STAT5 has significant difference between APS group and the control group at 4 time points. JAK2, STAT5 expressed in cytoplasm and nuclear of APS group significantly increased as compared to those of control group, and they expressed the strongest at 5 min.


CONCLUSIONS
JAK2, STAT5 signal transduction molecule plays an important role in the effect of APS cooperated with Epo on promoting hematopoiesis.
Hua
Zisen
Z
Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China. zisen526@yahoo.cn
Song
Shudan
S
Luo
Chunyan
C
Wang
Jianwei
J
Wang
Yaping
Y
D029969:Angelica / Q000737:chemistry*; D015153:Blotting, Western; D002478:Cells, Cultured; D004921:Erythropoietin / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006410:Hematopoiesis / Q000187:drug effects; D006801:Humans; D053614:Janus Kinase 2 / Q000378:metabolism*; D018613:Microscopy, Confocal; D011134:Polysaccharides / Q000494:pharmacology*; D050799:STAT5 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
D011134:Polysaccharides; D050799:STAT5 Transcription Factor; D004921:Erythropoietin; D053614:Janus Kinase 2