title: Chemical synthesis of homogeneous glycopeptides and glycoproteins.
abstract: Oligosaccharides linked to proteins are known to play important roles in several biological events. However, oligosaccharides are heterogeneous, which has hindered detailed elucidation of oligosaccharide functions. In order to solve this problem, glycoproteins having homogeneous oligosaccharides have long been required. For this purpose, an efficient preparative method of complex-type oligosaccharides has been investigated from a natural source and this method was found to afford over 24 kinds of diverse complex-type oligosaccharides by use of chemical methods and branch-specific sequential glycosidase digestion. The sufficient amount of homogeneous complex type oligosaccharides obtained enabled us to examine the synthesis of homogeneous glycopeptides as well as glycoproteins by use of solid phase glycopeptide synthetic method and native chemical ligation. This review describes recent progress related to the efficient method of oligosaccharide preparation and synthesis of glycoproteins including bioactive erythropoietin.
authors.affiliation: International Graduate School of Arts and Sciences, Yokohama City University, 22-2 Seto; Kanazawaku, Yokohama, 236-0027 Japan. kajihara@chem.sci.osaka-u.ac.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kajihara
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Ryo
authors.initials: R
authors.lastname: Okamoto
authors.forename: Kiriko
authors.initials: K
authors.lastname: Hirano
authors.forename: Takefumi
authors.initials: T
authors.lastname: Murase
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002621:Chemistry / Q000379:methods*; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D006023:Glycoproteins / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D006020:Glycopeptides; D006023:Glycoproteins