Erythrocytes as carrier for prednisolone: in vitro and in vivo evaluation.
Resealed erythrocytes, as drug delivery system has tremendous potential to achieve site specificity and prolonged release of drug thereby enhancing therapeutic index and patient compliance. In the present investigation erythrocytes obtained from healthy volunteers were loaded with prednisolone using preswell dilution and dilution technique with two different cross-linking agents, glutaraldehyde and dimethylsulphoxide. Carrier erythrocytes, having acceptable loading parameters showed increased percentage drug content with the addition of cross-linking agents. In vitro drug release followed zero-order kinetics, haemoglobin content was found to be satisfactory and osmotic fragility study indicated that increased drug entrapment efficiency was found at 0.3%w/v concentration of sodium chloride (hypotonic solution). In vivo tissue distribution studies were carried out for optimized formulation and order of distribution was found to be Liver>Lung>Kidney> Spleen. The developed drug delivery system is endowed with several exclusive advantages and hence holds potential for further research and clinical application.
Shavi
Gopal V
GV
Department of Pharmaceutics, KLES's College of Pharmacy, Belgaum-10, Karnataka State, India.
Doijad
Rajendra C
RC
Deshpande
Praful B
PB
Manvi
F V
FV
Meka
Sreenivasa Reddy
SR
Udupa
Nayanabhirama
N
Omprakash
R
R
Dhirendra
K
K
D003432:Cross-Linking Reagents / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics*; D004339:Drug Compounding / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D004912:Erythrocytes / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009996:Osmotic Fragility; D009997:Osmotic Pressure / Q000187:drug effects; D011239:Prednisolone / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D003432:Cross-Linking Reagents; D004337:Drug Carriers; D006454:Hemoglobins; D011239:Prednisolone; D005976:Glutaral; D004121:Dimethyl Sulfoxide