Identification and characterization of Th cell epitopes in MrkD adhesin of Klebsiella pneumoniae.
In this study, we identified the Th epitopes in MrkD of Klebsiella pneumoniae, an excellent vaccine candidate antigen. By using the RANKPEP prediction algorithm, we have identified and characterized three Th epitopes within the MrkD antigen, which can be recognized by CD4+ T cells from BALB/c (H-2(d)) mice. They were M(221-235), M(175-189), and M(264-278). These epitopes have important value for studying the immune response of K. pneumoniae infection and for designing effective vaccine against K. pneumoniae.
Li
Yang
Y
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Li
Zhi-jie
ZJ
Han
Wen-yu
WY
Lei
Lian-cheng
LC
Sun
Chang-jiang
CJ
Feng
Xin
X
Du
Chong-tao
CT
Du
Tao-feng
TF
Gu
Jing-min
JM
D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D018604:Epitope Mapping; D005260:Female; D036781:Fimbriae Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007710:Klebsiella Infections / Q000276:immunology* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D018829:Adhesins, Bacterial; C058768:Klebsiella pneumoniae type 3 fimbrial adhesin; D036781:Fimbriae Proteins