Counter-current chromatographic separation of nucleic acid constituents with a hydrophilic solvent system.
Nucleic acid constituents such as nucleobases, nucleosides and nucleotides were separated by counter-current chromatography using type J coil planet centrifuge. The separation was performed with a hydrophilic solvent system composed of 1-propanol/800 mM potassium phosphate buffer (pH 7.4) (1:1, v/v) by eluting the lower aqueous phase at a flow-rate of 0.5 ml/min. Eight selected nucleic acid constituents (4.0 mg, 0.5 mg of each), uridine monophosphate (UMP), adenosine monophosphate (AMP), deoxyadenosine monophosphate (dAMP), uridine, urasile, deoxy uridine, adenosine and adenine were well resolved within 160 min.
Shibusawa
Yoichi
Y
Division of Pharmaceutical and Biomedical Analysis, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. sibusawa@toyaku.ac.jp
Shoji
Atsushi
A
Suzuka
Chihiro
C
Yanagida
Akio
A
Ito
Yoichiro
Y
D003377:Countercurrent Distribution / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D009696:Nucleic Acids / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry*
D009696:Nucleic Acids; D012997:Solvents