Rapid profiling and target analysis of principal components in Fuling Decoctions by UFLC-DAD-ESI-MS.
The traditional Chinese medicine formula Fuling Decoction (FD) has been clinically used for eczema treatment, but the unclear chemical distribution and the lack of quality control have strongly restricted its application. In this study, an analytical method incorporating ultra-fast liquid chromatography (UFLC) with MS and UV detection was developed for rapid profiling of the chemical constitutes from FD. Fourteen constitutes were identified by UFLC-ESI-MS, while four major components including genipingentiobioside, geniposide, paeoniflorin and liquiritin were quantified simultaneously by UFLC-DAD. The UFLC-based method was fully validated and can be applied to screening and determination of principal components in commercially FD prescriptions.
Wang
Ao-Xue
AX
The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
Ge
Guang-Bo
GB
Qi
Xiao-Yi
XY
Hu
Ying
Y
Liang
Si-Cheng
SC
Yang
Ling
L
Tu
Cai-Xia
CX
D001565:Benzoates / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D044950:Flavanones / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D039823:Iridoids / Q000032:analysis*; D039821:Monoterpenes; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; D039823:Iridoids; D039821:Monoterpenes; C007835:geniposide; C015423:peoniflorin; D006027:Glycosides; C058967:genipin 1-gentiobioside; C512196:liquiritin