Creation of highly functional CO3Ap-collagen scaffold biomaterials.
Physicochemical properties of apatites are affected dramatically by the substitution of trace elements. Especially, biological apatites constituting bone and teeth contain several wt% of CO(3)(2- )ions, which are related to the crystallinity and solubility. Recently, scaffold biomaterials are being developed with a shape-maintaining property in addition to large pores and high porosity, into which cells can easily invade. To develop a new biodegradable scaffold biomaterial, bone-like carbonate apatites (CO(3)Ap) were synthesized and CO(3)Ap-collagen scaffolds were created. This scaffold biomaterial is useful for regions with bone regeneration ability. When these sponge-frame complexes with rh-BMP2 were implanted beneath the periosteum cranii of rats, sufficient new bone was created at the surface of the periosteum cranii after 4 weeks' implantation. Furthermore, when a CO(3)Ap-collagen sponge containing SVVYGLR peptide was implanted as a graft into a tissue defect created in rat tibia, the migration of numerous vascular endothelial cells, as well as prominent angiogenesis inside the graft, could be detected after 1 week. Thus, the modification of higher functions such as cytokine and angiogenesis factors is effective for low regeneration regions using tissue engineering biomaterials.
Okazaki
Masayuki
M
Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. okazakix@hiroshima-u.ac.jp
D000595:Amino Acid Sequence; D043925:Angiogenesis Inducing Agents / Q000494:pharmacology; D000818:Animals; D001031:Apatites / Q000138:chemical synthesis*; D001861:Bone Regeneration / Q000187:drug effects*; D003094:Collagen; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D020778:Matrix Metalloproteinase 2 / Q000494:pharmacology*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology; D053495:Osteopontin / Q000737:chemistry; D011817:Rabbits; D051381:Rats; D011994:Recombinant Proteins; D054457:Tissue Scaffolds / Q000737:chemistry; D014131:Trace Elements / Q000494:pharmacology
D043925:Angiogenesis Inducing Agents; D001031:Apatites; D009842:Oligopeptides; D011994:Recombinant Proteins; D014131:Trace Elements; D053495:Osteopontin; C030782:carboapatite; D003094:Collagen; D020778:Matrix Metalloproteinase 2; D008274:Magnesium