title: [Phenylhexyl isothiocyanate (PHI) regulates histone methylation and acetylation and induces apoptosis in SMMC-7721 cells].
abstract: OBJECTIVE
  To investigate the effects of PHI on histone acetylation and methylation in hepatocellular carcinoma line SMMC-7721 cells.
  
  
  METHODS
  Apoptosis was measured by TUNNEL assay. Histone methylation and acetylation were detected by Western blot.
  
  
  RESULTS
  PHI inhibited cells growth and induced apoptosis. PHI treatment resulted in increased acetylation of histone H3 and H4 , elevated level of histone H3 lysine 4 methylation, and decreased level of histone H3 lysine 9 methylation.
  
  
  CONCLUSIONS
  PHI can modulate both histone acetylation and methylation, which could remodel chromatin structure. PHI may be a novel anticancer drug.
authors.affiliation: Department of Hematology, Zhangzhou Affiliated Hospital of Fujian Medical University, Fujian Zhangzhou 363000, China.
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Huang
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Ma
authors.forename: Ya-Dong
authors.initials: YD
authors.lastname: Lai
authors.forename: Xiao-Zhong
authors.initials: XZ
authors.lastname: Wang
authors.forename: Jen-Wei
authors.initials: JW
authors.lastname: Chiao
authors.forename: De-Long
authors.initials: DL
authors.lastname: Liu
mesh_terms: D000107:Acetylation; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D008745:Methylation
chemical_list: D006657:Histones; D017879:Isothiocyanates; C073279:6-phenylhexyl isothiocyanate