Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis.
To improve production of ethanol from glycerol, the methylotrophic yeast Hansenula polymorpha was engineered to express the pdc and adhB genes encoding pyruvate decarboxylase and aldehyde dehydrogenase II from Zymomonas mobilis, respectively, under the control of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. The ethanol yield was 3.3-fold higher (2.74 g l(-1)) in the engineered yeast compared with the parent strain (0.83 g l(-1)). Further engineering to stimulate glycerol utilization in the recombinant strain via expression of dhaD and dhaKLM genes from Klebsiella pneumoniae encoding glycerol dehydrogenase and dehydroxyacetone kinase, respectively, resulted in a 3.7-fold increase (3.1 g l(-1)) in ethanol yield.
Hong
Won-Kyung
WK
Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk 580-185, South Korea.
Kim
Chul-Ho
CH
Heo
Sun-Yeon
SY
Luo
Lian Hua
LH
Oh
Baek-Rock
BR
Seo
Jeong-Woo
JW
D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D005990:Glycerol / Q000378:metabolism*; D010843:Pichia / Q000201:enzymology / Q000235:genetics; D010957:Plasmids; D011767:Pyruvate Decarboxylase / Q000235:genetics / Q000378:metabolism*; D017355:Zymomonas / Q000201:enzymology / Q000235:genetics
D000431:Ethanol; D000444:Aldehyde Dehydrogenase; D011767:Pyruvate Decarboxylase; D005990:Glycerol