[Characteristics of CDR3 of TCR beta on CD8+ T cells in chronic hepatitis B patients].
OBJECTIVE
To analyze the characteristics of CDR3 of TCRbeta on CD8+ T cells in chronic hepatitis B patients.


METHODS
Eight patients with chronic hepatitis B (ALT more than 2 ULN) were enrolled in this study. CD8+ T cells were isolated from peripheral blood. RT-PCR was proformed to amplify the CDR3 of TCRbeta, and the PCR products were sequenced and analyzed.


RESULTS
The chronic hepatitis B patients showed obvious clonal expansion of T cell, and three perturbation patterns of T cell expansion were showed in the CDR3 of TCRbeta, including monoclonicity, oligoclonicity and skewed peak patterns. The number of perturbation families of CD8+ subpopulation was significantly higher than that of CD8- subpopulation (10.6+/-4.7 vs. 4.1+/-3.1, t = 6.619, P less than 0.01). In 3 out of 8 patients, the number of perturbation families of CD8+ subpopulation was also higher than that of PBMCs without depleting CD8+ subpopulation.


CONCLUSIONS
The characteristics of CDR3 of TCRbeta may help to understand the inflammatory response in CHB patients.
Li
Yong-Yin
YY
Department of Infectious Disease, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Ma
Shi-Wu
SW
Zhang
Guang-Wen
GW
Huang
Xuan
X
Hu
Xiao-Xiong
XX
Yang
Ling
L
Zhang
Ke
K
Hou
Jin-Lin
JL
D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D022801:Complementarity Determining Regions / Q000235:genetics*; D019674:Genes, T-Cell Receptor beta; D019694:Hepatitis B, Chronic / Q000097:blood* / Q000276:immunology*; D006801:Humans; D008297:Male; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D055815:Young Adult
D022801:Complementarity Determining Regions; D011948:Receptors, Antigen, T-Cell