title: Two closely related subclass II SnRK2 protein kinases cooperatively regulate drought-inducible gene expression.
abstract: The subclass III group of SNF1-related protein kinase 2 (SnRK2) members is known to play an important role in ABA and osmotic stress signaling in Arabidopsis; however, the roles of other subclasses remain elusive. Here, we established a double mutant of SRK2C/SnR2.8 and SRK2F/SnRK2.7 to investigate the functions of subclass II SnRK2s. Microarray analysis suggested that subclass II SnRK2s regulate some drought-responsive genes involving ABA-responsive element binding transcription factors (AREB/ABF) and their targets, and quantitative reverse transcription- PCR confirmed that those genes were down-regulated significantly in srk2cf. This study indicates that subclass II SnRK2s also play important roles in drought stress signaling in Arabidopsis.
authors.affiliation: RIKEN Plant Science Center, 3-1-1, Kouyadai, Tsukuba, Ibaraki, 305-0074 Japan.
authors.forename: Masahide
authors.initials: M
authors.lastname: Mizoguchi
authors.forename: Taishi
authors.initials: T
authors.lastname: Umezawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nakashima
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kidokoro
authors.forename: Hironori
authors.initials: H
authors.lastname: Takasaki
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Fujita
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D018749:RNA, Plant; C492978:SnRK2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases