title: Actin and phosphoinositide recruitment to fully formed Candida albicans phagosomes in mouse macrophages.
abstract: Candida albicans is a dimorphic yeast that enters macrophages (Mphi) via the beta-glucan receptor dectin-1. Phagocytosis of C. albicans is characterized by actin polymerization, Syk kinase activation and rapid acquisition of phagolysosomal markers. In mice, C. albicans are able to resist the harsh environment of the phagosome and form pseudohyphae inside the phagolysosomal compartment, eventually extending from the Mphi. In this study, we investigated these unique C. albicans phagosomes and found that actin localized dynamically around the phagosomes, before disintegrating. Membrane phosphoinositides, PI(4,5)P(2), PI(3,4,5)P(3), PI(3,4)P(2), and PI(3)P also localized to the phagosomes. Localization was not related to actin polymerization, and inhibitor studies showed that polymerization of actin on the C. albicans phagosome was independent of PI3K. The ability of mature C. albicans phagosomes to stimulate actin polymerization could facilitate the escape of the growing yeast from the Mphi.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
authors.forename: Sigrid E M
authors.initials: SE
authors.lastname: Heinsbroek
authors.forename: Lynn A
authors.initials: LA
authors.lastname: Kamen
authors.forename: Philip R
authors.initials: PR
authors.lastname: Taylor
authors.forename: Gordon D
authors.initials: GD
authors.lastname: Brown
authors.forename: Joel
authors.initials: J
authors.lastname: Swanson
authors.forename: Siamon
authors.initials: S
authors.lastname: Gordon
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002176:Candida albicans / Q000276:immunology*; D002460:Cell Line; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure*; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010587:Phagocytosis; D010588:Phagosomes / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D010716:Phosphatidylinositols / Q000378:metabolism*
chemical_list: D000199:Actins; D010716:Phosphatidylinositols