Imaging of surfaces by concurrent surface plasmon resonance and surface plasmon resonance-enhanced fluorescence.
Surface plasmon resonance imaging and surface plasmon induced fluorescent are sensitive tools for surface analysis. However, existing instruments in this area have provided limited capability for concurrent detection, and may be large and expensive. We demonstrate a highly cost-effective system capable of concurrent surface plasmon resonance microscopy (SPRM) and surface plasmon resonance-enhanced fluorescence (SPRF) imaging, allowing for simultaneous monitoring of reflectivity and fluorescence from discrete spatial regions. The instrument allows for high performance imaging and quantitative measurements with surface plasmon resonance, and surface plasmon induced fluorescence, with inexpensive off-the-shelf components.
Thariani
Rahber
R
Department of Bioengineering, University of Washington, Seattle, Washington, United States of America. thariani@u.washington.edu
Yager
Paul
P
D001681:Biological Assay / Q000379:methods; D002138:Calibration; D003362:Cost-Benefit Analysis; D004867:Equipment Design; D008027:Light; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D012542:Scattering, Radiation; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties