[The influence of Bordetella pertussis bvgAS operon on formation and resolution of plasmid-chromosome cointegrates in Escherichia coli K12 mutants with damages in common components of the phosphoenolpyruvate:sugar phosphotransferase system].
The plasmids containing the genetically marked variants of Bordetela pertussi transposon TnBP were synthesized on the base of the plasmid with thermosensitive replication. The integration frequency of these plasmids into the E.coli K12 chromosome at non-permissive temperature (42 degrees C) was determined. It was found that the frequency of forming of RSBP-induced plasmid-chromosome cointegrated in bacteria E.coli K12 deficient in HPr or Enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system was decreased. The bvgAS operon from B.pertussis in trans-position restores the ability of mutant E.coli K12 to form and resolve.
Umiarov
A M
AM
Siniashina
L N
LN
Amelina
I P
IP
Datsenko
K A
KA
Bol'shakova
T N
TN
Dobrynina
O Iu
OIu
Karataev
G I
GI
D001426:Bacterial Proteins / Q000235:genetics*; D001886:Bordetella pertussis / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D048168:Escherichia coli K12 / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009876:Operon / Q000235:genetics*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D017354:Point Mutation; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C065601:BvgA protein, Bacteria; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D009004:Monosaccharide Transport Proteins; D014157:Transcription Factors; C080662:bvgS protein, Bordetella pertussis; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C022358:mannitol PTS permease, E coli; C030182:phosphocarrier protein HPr