Isolation of outer membrane proteins (OMP) from Salmonella cells using zwitterionic detergent and their separation by two-dimensional electrophoresis (2-DE).
We report that using the zwitterionic detergent Zwittergent Z 3-14 to isolate outer membrane proteins (OMPs) from Salmonella 048 is suitable for their separation by two-dimensional electrophoresis (2-DE) in a capillary tube system. Sample preparation is a very crucial step for any bacterial proteomic study. Some modifications were introduced to the 2-DE protocol suggested by O'Farrell and BioRad, which significantly impaired the resolution of proteins. 2-DE analysis of OMPs may be helpful in the interpretation of the variable susceptibility of Salmonella 048 rods to the bactericidal activity of serum.
Futoma-Kołoch
Bozena
B
Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Poland. bozena.futoma-koloch@microb.uni.wroc.pl
Bugla-Płoskońska
Gabriela
G
Doroszkiewicz
Włodzimierz
W
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D012475:Salmonella / Q000166:cytology* / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D003902:Detergents