title: Genetic variation of myeloperoxidase gene contributes to aggressive periodontitis: a preliminary association study in Turkish population.
abstract: Myeloperoxidase (MPO) is a lysosomal enzyme found in the azurophilic granules of polymorphonuclear leukocytes. It is involved in the defense against periodontal bacteria, and is also able to mediate inflammatory tissue destruction in aggressive and chronic periodontitis. The aim of this study was to explore the association between MPO-463G/A gene polymorphism and aggressive periodontitis (AgP) and chronic periodontitis (CP). The study included 147 subjects. Probing depth (PD), clinical attachment loss (CAL), plaque index (PI), and gingival index (GI) were recorded as the clinical parameters. Genomic DNA was obtained from the peripheral blood of 32 subjects with AgP, 25 with CP, and 90 reference controls. We genotyped the MPO-463G/A polymorphism using the PCR-RFLP method. All data were analyzed using SPSS version 13.0 for windows. There were no significant differences between the CP patients and controls regarding MPO-463A/G gene polymorphism either in terms of allele frequency or genotype frequency of MPO-463A/G. However, either in terms of allele frequency or genotype frequency of MPO-463A/G, there were significant differences between the AgP patients and the controls. In conclusion, our data suggest that MPO-463G/A may be associated with increased risk of aggressive periodontitis in Turkish patients.
authors.affiliation: Department of Periodontology,Gaziantep University Faculty of Dentistry, 27310 Gaziantep, Turkey. kmlercys@hotmail.com
authors.forename: Kamile
authors.initials: K
authors.lastname: Erciyas
authors.forename: Sacide
authors.initials: S
authors.lastname: Pehlivan
authors.forename: Tugce
authors.initials: T
authors.lastname: Sever
authors.forename: Recep
authors.initials: R
authors.lastname: Orbak
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000201:enzymology* / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000201:enzymology / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009195:Peroxidase / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D014421:Turkey; D055815:Young Adult
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D009195:Peroxidase