Rapid degradation of N-3-oxo-acylhomoserine lactones by a Bacillus cereus isolate from Malaysian rainforest soil.
A bacterial strain, KM1S, was isolated from a Malaysian rainforest soil sample by using a defined enrichment medium that specifically facilitates selection of quorum quenching bacteria. KM1S was clustered closely to Bacillus cereus by 16S ribosomal DNA sequence analysis. It degraded N-3-oxo-hexanoyl homoserine lactone and N-3-oxo-octanoyl homoserine lactone in vitro rapidly at 4.98 and 6.56 microg AHL h(-1) per 10(9) CFU/ml, respectively, as determined by the Rapid Resolution Liquid Chromatography. The aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of N-acylhomoserine lactones, of KM1S was amplified and cloned. Sequence analysis indicated the presence of the motif (106)HXDH-59 amino acids-H(169)-21 amino acids-D(191) for N-acylhomoserine lactone lactonases.
Chan
Kok-Gan
KG
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. kokgan@um.edu.my
Wong
Cheng-Siang
CS
Yin
Wai-Fong
WF
Sam
Choon-Kook
CK
Koh
Chong-Lek
CL
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D054742:Acyl-Butyrolactones / Q000378:metabolism*; D000595:Amino Acid Sequence; D001409:Bacillus cereus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D002861:Chromobacterium / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria; D008296:Malaysia; D008969:Molecular Sequence Data; D053038:Quorum Sensing; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014197:Trees
D054742:Acyl-Butyrolactones; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C088386:homoserine lactone; D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase; D015107:4-Butyrolactone