title: [Determination of ellagic acid compounds in Euscaphis japonica by using UPLC].
abstract: OBJECTIVE
  To develop an ultra performance liquid chromatography (UPLC) method for simultaneous determination of five ellagic acids in Euscaphis japonica.
  
  
  METHODS
  Analysis was carried out on an Acquity BEH C18 (2.1 mm x 100 mm, 1.7 microm) column at 40 degrees C eluted with acetonitrile-0.1% phosphoric acid aqueous solution as mobile phases in gradient elution. The flow rate was 0.2 mL x min(-1), and the detection wavelength set was monitored at 245 nm.
  
  
  RESULTS
  All calibration curves showed good linear relationship within test ranges (r >0.9997), and the overall recoveries were in the range of 97.2%-102.1%, with RSD less than 3.2% (n = 6). The overall RSD of precision test were less than 2.9%.
  
  
  CONCLUSIONS
  The developed method was simple, rapid, accurate and reproducible, and can be used for the quality control of E. japonica.
authors.affiliation: School of Pharmacy, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Aimin
authors.initials: A
authors.lastname: Wang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.forename: Haijun
authors.initials: H
authors.lastname: Wang
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Li
authors.forename: Shanggao
authors.initials: S
authors.lastname: Liao
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002853:Chromatography, Liquid; D004610:Ellagic Acid / Q000737:chemistry*; D019684:Magnoliopsida / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D004610:Ellagic Acid