title: Ligamentization of tendon grafts treated with an endogenous preparation rich in growth factors: gross morphology and histology.
abstract: OBJECTIVE
  To investigate whether the application of a particular platelet-rich plasma preparation rich in growth factors (PRGF) during anterior cruciate ligament (ACL) surgery gives a potential advantage for better tendon graft ligamentization.
  
  
  METHODS
  This study included 37 volunteers who underwent either conventional (control group, n = 15) or PRGF-assisted (n = 22) ACL reconstruction with an autogenous hamstring and required second-look arthroscopy to remove hardware or loose bodies, treat meniscal tears or plica syndrome, or resect cyclops lesions at 6 to 24 months after ACL surgery. The gross morphologies of the grafts were evaluated on second-look arthroscopy by use of the full arthroscopic score (0 to 4 points) to evaluate graft thickness and apparent tension (0 to 2 points) plus synovial coverage (0 to 2 points). At the same time, biopsy specimens were harvested uniformly from the grafted tendons. In these specimens the histologic transformation of the tendon graft to ACL-like tissue was evaluated by use of the Ligament Tissue Maturity Index, and a score to assess the progression of new connective tissue enveloping the graft was created by use of 3 criteria previously used to characterize changes during ligament healing: cellularity, vascularity, and collagen properties.
  
  
  RESULTS
  The overall arthroscopic evaluation of PRGF-treated grafts showed an excellent rating in 57.1% of the knees (score of 4) and a fair rating in 42.9% (score of 2 or 3). In contrast, evaluation of untreated grafts showed an excellent rating in 33.3% of the knees, a fair rating in 46.7%, and a poor rating in 20% (score of 0 or 1). Overall, arthroscopic evaluations were not statistically different between PRGF and control groups (P = .051). PRGF treatment influenced the histologic characteristics of the tendon graft, resulting in tissue that was more mature than in controls (P = .024). Histologically evident newly formed connective tissue enveloping the graft was present in 77.3% of PRGF-treated grafts and 40% of controls. The appearance of the connective tissue envelope changed with increasing time from surgery. On the basis of the histologic findings, we suggest that the remodeling of PRGF-treated grafts involves the formation of synovial-like tissue enveloping the graft. This tissue is eventually integrated in the remodeled tendon graft, conferring a similar appearance to the normal ACL.
  
  
  CONCLUSIONS
  The use of PRGF influenced the histologic characteristics of tendon grafts, resulting in more remodeling compared with untreated grafts. We have shown temporal histologic changes during the 6- to 24-month postoperative period of graft maturation, with newly formed connective tissue enveloping most grafts treated with PRGF.
  
  
  METHODS
  Level III, case-control study.
authors.affiliation: Unidad de Cirugía Artroscópica Mikel Sánchez, Clínica USP-La Esperanza, Vitoria-Gasteiz, Spain.
authors.forename: Mikel
authors.initials: M
authors.lastname: Sánchez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Anitua
authors.forename: Juan
authors.initials: J
authors.lastname: Azofra
authors.forename: Roberto
authors.initials: R
authors.lastname: Prado
authors.forename: Francisco
authors.initials: F
authors.lastname: Muruzabal
authors.forename: Isabel
authors.initials: I
authors.lastname: Andia
mesh_terms: D000293:Adolescent; D000328:Adult; D016118:Anterior Cruciate Ligament / Q000187:drug effects* / Q000473:pathology* / Q000601:surgery; D001182:Arthroscopy; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D007719:Knee Joint / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D053657:Platelet-Rich Plasma; D013710:Tendons / Q000187:drug effects* / Q000473:pathology* / Q000637:transplantation; D055815:Young Adult
chemical_list: D036341:Intercellular Signaling Peptides and Proteins