[Enhancing ethanol production using thermophilic yeast by response surface methodology].
We optimized the conditions of simultaneous saccharification and fermentation (SSF) from cassava flour into high-concentration ethanol by thermophilic yeast GXASY-10. Based on the single factor experiment, we screened the important parameters by Plackeet-burman design. We used the path of steepest ascent to approach to the biggest region of ethanol production subsequently. Then, we obtained the optimum values of the parameters by Box-Behnken design. The results showed that the important parameters were the liquefaction time, glucosidase dosages and initial concentration of cassava flour (substrate). The optimum technical conditions were as follows: liquefaction time 35 min, glucosidase dosages 1.21 AGU/g substrate and initial substrate concentration 37.62%. Under such optimum conditions, the ethanol yield of 20 L fermentor reached 16.07% (V/W) after 48 h fermentation at 37 degrees C and 100 r/min. The ethanol content increased 33% than that under the original condition.
Shen
Naikun
N
National Engineering Research Center for Non-food Biorefinery, Guangxi Academy ofSciences, Nanning 530007, China.
Wang
Qingyan
Q
Lu
Yan
Y
Qin
Yan
Y
Huang
Ribo
R
D000431:Ethanol / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation; D005959:Glucosidases / Q000494:pharmacology; D006358:Hot Temperature; D002365:Manihot / Q000378:metabolism*; D013379:Substrate Specificity; D015003:Yeasts / Q000502:physiology*
D000431:Ethanol; D005959:Glucosidases