[Uniform designed research on the active ingredients assembling of Chinese medicine prescription for anti-liver fibrosis].
OBJECTIVE
To explore the method and significance for studying active anti-liver fibrosis ingredients consisted Chinese medicine compound prescription based on Chinese medicine theory.


METHODS
Optimized prescription was screened out, adopting uniform block design with 4-factor 8-level table and regression analysis, through applying the four known effective ingredients (cordyceps sinensis polysaccharide, salvianolic acid B, amygdaloside and gypenosides) of Fuzheng Huayu Capsule (FZHYC, a new Chinese medine anti-liver fibrosis drug) to two rat liver fibrosis models established separately by dimethylnitrosamine (DMN) and CCl4, and taking the liver content of hydroxyproline (Hyp) as the screen index. Then a further study for comparing and verifying the efficacy of the obtained optimized prescription was conducted on the two former models respectively by observing the changes of Hyp content in liver, serum ALT activity and fibrosis pathology after medication, controlled by the original FZHYC and the recipe assembled by all the four ingredients.


RESULTS
Two optimized prescriptions (OPA and OPB) were screened out separately in the studies conducted on the two models. Both of them were consisted of cordyceps sinensis polysaccharide, Amygdaloside and Gypenosides, but different in constituent ratio, i.e., the ratio in OPA was 60 : 80: 50, and that in OPB, 20: 160: 50. Verifying study showed both OPA and OPA were significantly effective, with the efficacy equivalent to that of FZHYC (P>0.05). However, when they were used in combining with salvianolic acid B (the cutout ingredient in the screening), the efficacy lowered surely.


CONCLUSIONS
Uniform design is a valuable method in the compatibility research of Chinese Medicine drugs' composition. To assemble a new compound recipe reasonably based on the prescription of traditional compound recipe could make its effect equivalent to that of the original prescription. Ingredients or constituents in a prescription, either presented synergistic or antagonistic effects, are not randomly stacked together, and they should be orderly assembled in intrinsic rules of qualitative and quantitative changing.
Li
Xue-mei
XM
Shuguang Hospital & Institute of Liver Disease, Shanghai University of Traditional Chinese Medicine, Shanghai 201203.
Hu
Yi-yang
YY
Duan
Xiao-hua
XH
D000678:Amygdalin / Q000008:administration & dosage; D000818:Animals; D004338:Drug Combinations; D015195:Drug Design; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000627:therapeutic use; D029774:Gynostemma; D008103:Liver Cirrhosis / Q000188:drug therapy; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C532829:fuzheng huayu; C474918:gypenoside; D000678:Amygdalin