title: Oxidative stress markers in patients with primary open-angle glaucoma.
abstract: OBJECTIVE
  To investigate the levels of antioxidant enzymes catalase (CAT), glutathione peroxidase (GPO), superoxide dismutase (SOD), and malondialdehyde (MDA) in human eyes with primary open-angle glaucoma (POAG) and to correlate their concentrations with severity of glaucoma.
  
  
  METHODS
  A prospective cases control study.
  
  
  METHODS
  Thirty patients with primary open-angle glaucoma and twenty-five patients with senile cataracts of matched age and gender were included in the study prospectively. Aqueous humor samples were obtained by paracentesis at the time of elective surgery for glaucomatous and cataractous patients. Aqueous humor were analyzed for CAT, GPO, SOD, and MDA status.
  
  
  RESULTS
  GPO, SOD, and MDA enzyme levels revealed a high significant increase in aqueous humor of POAG patients with respect to the comparative group of cataract patients (P < 0.001). No significant difference in the activity of CAT enzyme in aqueous humor of POAG and cataract patient (P = 0.201). Significant correlation was found between the MDA enzyme level and severe visual field loss (P < 0.001) in POAG patients.
  
  
  CONCLUSIONS
  Increased levels of aqueous humor GPO, SOD, and MDA may be associated with POAG. In addition, they may be useful antioxidant enzyme levels in aqueous humor of POAG patients as a result of glaucoma disease and not a cause.
authors.affiliation: Department of Ophthalmology, Faculty of Medicine, Mansoura University, Egypt. asaadghanem@hotmail.com
authors.forename: Asaad A
authors.initials: AA
authors.lastname: Ghanem
authors.forename: Lamiaa F
authors.initials: LF
authors.lastname: Arafa
authors.forename: Ayman
authors.initials: A
authors.lastname: El-Baz
mesh_terms: D000328:Adult; D000975:Antioxidants; D001082:Aqueous Humor / Q000201:enzymology*; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D002374:Catalase / Q000378:metabolism; D002386:Cataract / Q000378:metabolism; D005260:Female; D005902:Glaucoma, Open-Angle / Q000201:enzymology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D018384:Oxidative Stress; D011446:Prospective Studies; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase