title: Comparison of a new commercial test, Dermatophyte-PCR kit, with conventional methods for rapid detection and identification of Trichophyton rubrum in nail specimens.
abstract: The performance of a new commercially available duplex PCR, which combines pan-dermatophyte PCR with a Trichophyton rubrum-specific PCR, was evaluated. This Dermatophyte PCR kit, which requires one day for laboratory diagnosis, was compared with the conventional methods of microscopy and culture that necessitate up to 4 weeks for final diagnosis of dermatophytosis. We studied 177 nail samples from patients with suspected onychomycosis by fluorescence microscopy (blankophore), cultures and the Dermatophyte PCR kit. More samples were positive by PCR (78/177, 44%) than by culture (59/177, 34%). T. rubrum was present in 95% of all culture-positive nail specimens, which was confirmed by PCR in 55/56 specimens. The positive predictive value, negative predictive value, specificity and sensitivity of the duplex PCR was 93%, 87%, 94% and 85%, respectively, when confirmed by positive culture, microscopy or both. Due to its sensitivity, specificity and rapidity, we conclude that this PCR is an attractive method for routine investigation of nail dermatophytosis in a clinical setting.
authors.affiliation: Department of Clinical Bacteriology, Sahlgrenska University Hospital, Göteborg, Sweden. nahid.kondori@microbio.gu.se
authors.forename: Nahid
authors.initials: N
authors.lastname: Kondori
authors.forename: Anna-Lena
authors.initials: AL
authors.lastname: Abrahamsson
authors.forename: Naser
authors.initials: N
authors.lastname: Ataollahy
authors.forename: Christine
authors.initials: C
authors.lastname: Wennerås
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D003937:Diagnosis, Differential; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods*; D009262:Nails / Q000382:microbiology*; D014009:Onychomycosis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014005:Tinea / Q000175:diagnosis* / Q000382:microbiology; D014249:Trichophyton / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic; D011933:Reagent Kits, Diagnostic