title: A first semimanual device for clinical intramuscular repetitive cell injections.
abstract: Intramuscular cell transplantation in humans requires so far meticulous repetitive cell injections. Performed percutaneously with syringes operated manually, the procedure is very time consuming and requires a lot of concentration to deliver the cells exactly in the required region. This becomes impractical and inaccurate for large volumes of muscle. In order to accelerate this task, to render it more precise, and to perform injections more reproducible in large volumes of muscle, we developed a specific semimanual device for intramuscular repetitive cell injections. Our prototype delivers very small quantities of cell suspension, homogeneously throughout several needles, from a container in the device. It was designed in order to deliver the cells as best as possible only in a given subcutaneous region (in our case, skeletal muscles accessible from the surface), avoiding wasting in skin and hypodermis. The device was tested in monkeys by performing intramuscular allotransplantations of beta-galactosidase-labeled myoblasts. During transplantations, it was more ergonomic and considerably faster than manually operated syringes, facilitating the cell graft in whole limb muscles. Biopsies of the myoblast-injected muscles 1 month later showed abundant beta-galactosidase-positive myofibers with homogeneous distribution through the biopsy sections. This is the first device specifically designed for the needs of intramuscular cell transplantation in a clinical context.
authors.affiliation: Robotics Laboratory, Department of Mechanical Engineering, Laval University, Quebec, Canada.
authors.forename: Pierre-Luc
authors.initials: PL
authors.lastname: Richard
authors.forename: Clément
authors.initials: C
authors.lastname: Gosselin
authors.forename: Thierry
authors.initials: T
authors.lastname: Laliberté
authors.forename: Martin
authors.initials: M
authors.lastname: Paradis
authors.forename: Marlyne
authors.initials: M
authors.lastname: Goulet
authors.forename: Jacques P
authors.initials: JP
authors.lastname: Tremblay
authors.forename: Daniel
authors.initials: D
authors.lastname: Skuk
mesh_terms: D000818:Animals; D001706:Biopsy; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation / Q000379:methods; D017690:Cell Transplantation / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004389:Durable Medical Equipment / Q000639:trends*; D004867:Equipment Design / Q000379:methods; D017930:Genes, Reporter; D006085:Graft Survival / Q000502:physiology; D006801:Humans; D007273:Injections, Intramuscular / Q000295:instrumentation / Q000379:methods; D007763:Lac Operon; D008252:Macaca fascicularis; D009135:Muscular Diseases / Q000628:therapy; D032446:Myoblasts / Q000166:cytology / Q000502:physiology / Q000637:transplantation; D033581:Stem Cell Transplantation / Q000295:instrumentation / Q000379:methods; D013594:Syringes / Q000639:trends*; D014184:Transplantation, Homologous / Q000295:instrumentation / Q000379:methods