Solanum torvum inhibits Helicobacter pylori growth and mediates apoptosis in human gastric epithelial cells.
Helicobacter pylori infection is associated with an increased risk for development of duodenal ulcers, gastric ulcers, gastric adenocarcinomas and gastric lymphomas. However, resistant strains have developed because of antibiotic treatment. In this study, the water, acetone, chloroform and methanol extracts of two Solancaceae plants, Solanum erianthum and Solanum torvum (ST), were tested for their anti-H. pylori activity. All of ST extracts were able to inhibit the growth of H. pylori and showed better activities against antibiotic strains than the reference strain. Among them, chloroform extract of ST (ST-C) possessed the strongest ability to inhibit H. pylori growth. Association assay was performed by the ST-C showing that ST-C was able to interrupt the association of bacteria to host cells. Furthermore, H. pylori-induced apoptosis could also be efficiently suppressed by the ST-C. It was able to interfere with the interaction between bacteria and host cells and also target H. pylori-induced gastric injury by suppressing apoptosis. Therefore, ST-C may offer a new approach for the treatment of H. pylori. Further studies on the elucidation of the molecular mechanisms of the growth inhibition on H. pylori by ST-C, and to identify active compounds in the plants are in progress.
Hsu
Yuan-Man
YM
Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
Weng
Jing-Ru
JR
Huang
Tsurng-Juhn
TJ
Lai
Chih-Ho
CH
Su
Chiu-Hsiang
CH
Chou
Chang-Hung
CH
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001422:Bacterial Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D052978:Disk Diffusion Antimicrobial Tests; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D032322:Solanum
D000900:Anti-Bacterial Agents; D051017:Apoptosis Regulatory Proteins; D010936:Plant Extracts