title: Proteomic identification of serum proteins associated with stress-induced gastric ulcers in fasted rats.
abstract: Several physical and psychological stresses frequently become triggers for gastrointestinal disorders such as ulcer. In this study, we tried to identify serum proteins as potential biomarkers for the evaluation of stress-induced gastric ulcer. By proteomic analysis using rats with gastric ulcer induced by water immersion and restraint (WIR) stress as an animal model, we found quantitative changes in several serum proteins, including creatine kinase muscle M chain (CK-M) and apolipoprotein A-IV (ApoA4) in the stressed rats. On western blotting and enzyme-linked immunosorbent assay (ELISA), we confirmed that serum CK-M was remarkably increased by WIR stress. However, ApoA4 appeared to be decreased by fasting, but not WIR stress, which is usually applied prior to WIR stress. The findings suggest that these two serum proteins might be useful as biomarkers, CK-M for stress-induced gastric ulcer and ApoA4 for starvation.
authors.affiliation: Graduate School of Nutritional and Environmental Sciences and Global COE Program, University of Shizuoka, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Kato
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Naitou
authors.forename: Makoto
authors.initials: M
authors.lastname: Namioka
authors.forename: Masanobu
authors.initials: M
authors.lastname: Akimoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ishii
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakakibara
authors.forename: Kayoko
authors.initials: K
authors.lastname: Shimoi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakayama
authors.forename: Norio
authors.initials: N
authors.lastname: Ohashi
mesh_terms: D000818:Animals; D001054:Apolipoproteins A; D001798:Blood Proteins / Q000378:metabolism; D004381:Duodenal Ulcer / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007101:Immersion; D008297:Male; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000378:metabolism*; D013315:Stress, Psychological / Q000150:complications*
chemical_list: D001054:Apolipoproteins A; D001798:Blood Proteins; D011506:Proteins; C022205:apolipoprotein A-IV