Effects of topical application of alpha-D-glucosylglycerol on dermal levels of insulin-like growth factor-i in mice and on facial skin elasticity in humans.
Sensory neurons release calcitonin-gene related peptide (CGRP) on stimulation. We have reported that topical application of capsaicin increases facial skin elasticity by increasing the production of dermal insulin-like growth factor-I (IGF-I) through stimulation of sensory neurons in mice and humans. In this study, we examined whether topical application of alpha-D-glucosylglycerol (GG), a compound found in Japanese traditional brewed foods such as sake (Japanese rice wine), increases the dermal production of IGF-I in mice and increases the facial skin elasticity in females. GG increased CGRP release and cAMP levels in dorsal root ganglion (DRG) neurons isolated from wild-type mice. Pretreatment with capsazepine, an inhibitor of vanilloid receptor-1 activation, and with KT5720, an inhibitor of protein kinase A, reversed GG-induced increases in CGRP release from DRG neurons. Topical application of GG increased dermal levels of IGF-I, IGF-I mRNA, and collagen in wild-type mice, but not in CGRP-knockout mice. Topical application of GG increased cheek-skin elasticity in 13 female volunteers. These observations strongly suggest that GG increases the production of IGF-I in the skin through sensory neuron stimulation, thereby increasing skin elasticity.
Harada
Naoaki
N
Department of Translational Medical Science Research, Nagoya City University Graduate School of Medical Sciences, Japan.
Zhao
Juan
J
Kurihara
Hiroki
H
Nakagata
Naomi
N
Okajima
Kenji
K
D000287:Administration, Topical; D000818:Animals; D002211:Capsaicin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D002227:Carbazoles; D020405:Dermis / Q000378:metabolism; D004548:Elasticity / Q000187:drug effects; D005145:Face; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism; D005796:Genes / Q000187:drug effects; D005960:Glucosides; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism; D011758:Pyrroles; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D050916:TRPV Cation Channels
D002227:Carbazoles; D005960:Glucosides; D011758:Pyrroles; D012333:RNA, Messenger; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; C034727:glucosylglycerol; C057416:KT 5720; D007334:Insulin-Like Growth Factor I; C071423:capsazepine; D002211:Capsaicin