[Phenylhexyl isothiocyanate (PHI) regulates histone methylation and acetylation and induces apoptosis in SMMC-7721 cells].
OBJECTIVE
To investigate the effects of PHI on histone acetylation and methylation in hepatocellular carcinoma line SMMC-7721 cells.


METHODS
Apoptosis was measured by TUNNEL assay. Histone methylation and acetylation were detected by Western blot.


RESULTS
PHI inhibited cells growth and induced apoptosis. PHI treatment resulted in increased acetylation of histone H3 and H4 , elevated level of histone H3 lysine 4 methylation, and decreased level of histone H3 lysine 9 methylation.


CONCLUSIONS
PHI can modulate both histone acetylation and methylation, which could remodel chromatin structure. PHI may be a novel anticancer drug.
Huang
Yi-Qun
YQ
Department of Hematology, Zhangzhou Affiliated Hospital of Fujian Medical University, Fujian Zhangzhou 363000, China.
Ma
Xu-Dong
XD
Lai
Ya-Dong
YD
Wang
Xiao-Zhong
XZ
Chiao
Jen-Wei
JW
Liu
De-Long
DL
D000107:Acetylation; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D008745:Methylation
D006657:Histones; D017879:Isothiocyanates; C073279:6-phenylhexyl isothiocyanate