Effect of different glucose concentrations on proteome of Saccharomyces cerevisiae.
We performed a proteomic study to understand how Saccharomyces cerevisiae adapts its metabolism during the exponential growth on three different concentrations of glucose; this information will be necessary to understand yeast carbon metabolism in different environments. We induced a natural diauxic shift by growing yeast cells in glucose restriction thus having a fast and complete glucose exhaustion. We noticed differential expressions of groups of proteins. Cells in high glucose have a decreased growth rate during the initial phase of fermentation; in glucose restriction and in high glucose we found an over-expression of a protein (Peroxiredoxin) involved in protection against oxidative stress insult. The information obtained in our study validates the application of a proteomic approach for the identification of the molecular bases of environmental variations such as fermentation in high glucose and during a naturally induced diauxic shift.
Guidi
Francesca
F
Dipartimento di Scienze Biochimiche, Università degli Studi di Firenze, Viale Morgagni, 50, 50134 Firenze, Italy.
Francesca
Guidi
G
Magherini
Francesca
F
Francesca
Magherini
M
Gamberi
Tania
T
Tania
Gamberi
G
Borro
Marina
M
Marina
Borro
B
Simmaco
Maurizio
M
Maurizio
Simmaco
S
Modesti
Alessandra
A
Alessandra
Modesti
M
D015180:Electrophoresis, Gel, Two-Dimensional; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D005609:Free Radicals; D005656:Fungal Proteins / Q000737:chemistry; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D005609:Free Radicals; D005656:Fungal Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D000431:Ethanol; D054464:Peroxiredoxins; D005947:Glucose