title: Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects.
abstract: The medical significance of circulating endothelial or hematopoietic progenitors is becoming increasing recognized. While therapeutic augmentation of circulating progenitor cells using G-CSF has resulted in promising preclinical and early clinical data for several degenerative conditions, this approach is limited by cost and inability to perform chronic administration. Stem-Kine is a food supplement that was previously reported to augment circulating EPC in a pilot study. Here we report a trial in 18 healthy volunteers administered Stem-Kine twice daily for a 2 week period. Significant increases in circulating CD133 and CD34 cells were observed at days 1, 2, 7, and 14 subsequent to initiation of administration, which correlated with increased hematopoietic progenitors as detected by the HALO assay. Augmentation of EPC numbers in circulation was detected by KDR-1/CD34 staining and colony forming assays. These data suggest Stem-Kine supplementation may be useful as a stimulator of reparative processes associated with mobilization of hematopoietic and endothelial progenitors.
authors.affiliation: Medistem Inc, San Diego, California, USA.
authors.forename: Nina A
authors.initials: NA
authors.lastname: Mikirova
authors.forename: James A
authors.initials: JA
authors.lastname: Jackson
authors.forename: Ron
authors.initials: R
authors.lastname: Hunninghake
authors.forename: Julian
authors.initials: J
authors.lastname: Kenyon
authors.forename: Kyle W H
authors.initials: KW
authors.lastname: Chan
authors.forename: Cathy A
authors.initials: CA
authors.lastname: Swindlehurst
authors.forename: Boris
authors.initials: B
authors.lastname: Minev
authors.forename: Amit N
authors.initials: AN
authors.lastname: Patel
authors.forename: Michael P
authors.initials: MP
authors.lastname: Murphy
authors.forename: Leonard
authors.initials: L
authors.lastname: Smith
authors.forename: Famela
authors.initials: F
authors.lastname: Ramos
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Ichim
authors.forename: Neil H
authors.initials: NH
authors.lastname: Riordan
mesh_terms: D000071916:AC133 Antigen; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D001681:Biological Assay; D002452:Cell Count; D002465:Cell Movement; D003114:Colony-Forming Units Assay; D019587:Dietary Supplements; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism; D010641:Phenotype; D055815:Young Adult
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides