title: A single coxsackievirus B2 capsid residue controls cytolysis and apoptosis in rhabdomyosarcoma cells.
abstract: Coxsackievirus B2 (CVB2), one of six human pathogens of the group B coxsackieviruses within the enterovirus genus of Picornaviridae, causes a wide spectrum of human diseases ranging from mild upper respiratory illnesses to myocarditis and meningitis. The CVB2 prototype strain Ohio-1 (CVB2O) was originally isolated from a patient with summer grippe in the 1950s. Later on, CVB2O was adapted to cytolytic replication in rhabdomyosarcoma (RD) cells. Here, we present analyses of the correlation between the adaptive mutations of this RD variant and the cytolytic infection in RD cells. Using reverse genetics, we identified a single amino acid change within the exposed region of the VP1 protein (glutamine to lysine at position 164) as the determinant for the acquired cytolytic trait. Moreover, this cytolytic virus induced apoptosis, including caspase activation and DNA degradation, in RD cells. These findings contribute to our understanding of the host cell adaptation process of CVB2O and provide a valuable tool for further studies of virus-host interactions.
authors.affiliation: School of Natural Sciences, Linnaeus University, SE-391 82 Kalmar, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Gullberg
authors.forename: Conny
authors.initials: C
authors.lastname: Tolf
authors.forename: Nina
authors.initials: N
authors.lastname: Jonsson
authors.forename: Charlotta
authors.initials: C
authors.lastname: Polacek
authors.forename: Jana
authors.initials: J
authors.lastname: Precechtelova
authors.forename: Miriam
authors.initials: M
authors.lastname: Badurova
authors.forename: Martin
authors.initials: M
authors.lastname: Sojka
authors.forename: Camilla
authors.initials: C
authors.lastname: Mohlin
authors.forename: Stina
authors.initials: S
authors.lastname: Israelsson
authors.forename: Kjell
authors.initials: K
authors.lastname: Johansson
authors.forename: Shubhada
authors.initials: S
authors.lastname: Bopegamage
authors.forename: Susan
authors.initials: S
authors.lastname: Hafenstein
authors.forename: A Michael
authors.initials: AM
authors.lastname: Lindberg
mesh_terms: D000818:Animals; D017209:Apoptosis; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002522:Chlorocebus aethiops; D029822:Enterovirus B, Human / Q000235:genetics / Q000378:metabolism*; D004769:Enterovirus Infections / Q000503:physiopathology* / Q000821:virology; D006801:Humans; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D012208:Rhabdomyosarcoma / Q000503:physiopathology* / Q000821:virology
chemical_list: D036022:Capsid Proteins