Development of real-time and nested RT-PCR to detect astrovirus and one-year survey of astrovirus in Jiangmen City, China.
Human astroviruses (HAstVs) are one of the major viral agents of acute gastroenteritis (AGE) in all age groups, especially in young children. In this study, new one-step real-time RT-PCR and nested RT-PCR assays were developed to detect HAstVs. HAstVs were identified in 46 (8.75%) of 526 stool samples in Jiangmen City over 1 year, including 43 (9.15%) of 470 children and 3 (5.4%) of 56 adults, and HAstV-1 was the most predominant strain. This finding suggests that HAstVs infections are common in Jiangmen City, China. Further detailed molecular epidemiological studies are required for understanding the prevalence of HAstVs infection and gaining knowledge about the circulating genotypes.
Dai
Ying-chun
YC
Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Tonghe Road, Guangzhou, Guang Dong, People's Republic of China.
Xu
Qiao-hua
QH
Wu
Xian-bo
XB
Hu
Gui-fang
GF
Tang
Ya-li
YL
Li
Jian-dong
JD
Chen
Qing
Q
Nie
Jun
J
D000328:Adult; D019350:Astroviridae Infections / Q000453:epidemiology* / Q000821:virology; D002648:Child; D002681:China / Q000453:epidemiology; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D001257:Mamastrovirus / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA