A lens-specific co-injection marker for medaka transgenesis.
Recent advances in generating transgenic fish have improved the efficiency of germline transmission and enabled the generation of large numbers of transgenic animals. A suitable co-injection marker may help facilitate the preselection of transgenic embryos. For this purpose, a lens-specific marker appears to be a suitable candidate since the lens is a well-defined tissue that is easily accessible for examination of reporter gene expression. We constructed reporter vectors including the mouse gamma-F crystallin (mgammaF-Cry) promoter, which drives high levels of lens-specific heterologous expression of the reporter gene and thereby enables easy sorting of transgenic fish.
Vopalensky
Pavel
P
Department of Transcriptional Regulation, Institute of Molecular Genetics Prague, Czech Republic.
Ruzickova
Jana
J
Pavlu
Barbora
B
Kozmik
Zbynek
Z
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics*; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D009990:Oryzias / Q000235:genetics*; D038222:gamma-Crystallins / Q000235:genetics*
D005819:Genetic Markers; D008164:Luminescent Proteins; D038222:gamma-Crystallins