The role of the lactadherin in promoting intestinal DCs development in vivo and vitro.
Lactadherin, as one of the immune components in the breast milk, might play a role in the intestinal immune system of newborn. Therefore, we investigated the effect of lactadherin-feeding in early time on the development of intestinal immune system compared with naturally rearing and artificially rearing (non-lactadherin). In the present study, we observed that the Peyer's Patches (PP) from the pups of artificially reared group with lactadherin added were characterized by an excess of OX62(+)CD4(+)SIRP(+) DC cells and a higher expression of CD3(+)CD4(+)CD25(+)T cells. Additionally, this study also demonstrated that IL-10 production was dramatically increased when lactadherin was present in culture medium compared with lactadherin-absent culture. These results suggested that lactadherin could adjust intestinal DCs activity, induce CD3(+)CD4(+)CD25(+)T cell differentiation, and enhance IL-10 production.
Zhou
Yi-Jun
YJ
Department of Pediatrics, Xinhua Hospital Affiliated to Medical Collage, Shanghai Jiaotong University, Shanghai, China.
Gao
Juan
J
Yang
Hua-Mei
HM
Yuan
Xiang-Liang
XL
Chen
Tong-Xin
TX
He
Zhen-Juan
ZJ
D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology; D000954:Antigens, Surface; D001942:Breast Feeding; D017252:CD3 Complex / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D007413:Intestinal Mucosa / Q000254:growth & development / Q000276:immunology*; D008892:Milk / Q000276:immunology*; D008894:Milk Proteins / Q000276:immunology*; D010581:Peyer's Patches / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000943:Antigens, Differentiation; D000954:Antigens, Surface; D017252:CD3 Complex; D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit; C499941:MFGE8 protein, rat; D008894:Milk Proteins; C459465:OX-62 antigen, rat; D016753:Interleukin-10