Application of bicellar systems on skin: diffusion and molecular organization effects.
The effect of bicelles formed by dipalmitoylphosphatidylcholine (DPPC)/dihexanoylphosphatidylcholine (DHPC) on stratum corneum (SC) lipids was studied by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy at different temperatures. Analysis of the lipid organization in terms of chain conformational order and lateral packing shows that the use of bicelles hampers the fluidification of SC lipids with temperature and leads to a lateral packing corresponding to a stable hexagonal phase. Grazing incidence small- and wide-angle X-ray scattering (GISAXS and GIWAXS) techniques confirm these results and give evidence of higher lamellar order after treatment with these bicelles. Additionally, the effects of DPPC/DHPC and dimyristoylphosphatidylcholine (DMPC)/DHPC bicelles at different SC depths were compared. The combination of ATR-FTIR spectroscopy and the tape-stripping method was very useful for this purpose.
Rodríguez
Gelen
G
Departament de Tecnologia Química i de Tensioactius, Institut de Quimica Avançada de Catalunya, Consejo Superior de Investigaciones Científicas, C/Jordi Girona 18-26, 08034 Barcelona, Spain. gelen.rodriguez@cid.csic.es
Rubio
Laia
L
Cócera
Mercedes
M
Estelrich
Joan
J
Pons
Ramon
R
de la Maza
Alfonso
A
López
Olga
O
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000818:Animals; D004817:Epidermis / Q000737:chemistry / Q000378:metabolism*; D066298:In Vitro Techniques; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010713:Phosphatidylcholines / Q000737:chemistry; D012542:Scattering, Radiation; D012867:Skin / Q000737:chemistry / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine; D013696:Temperature; D014965:X-Rays
D008051:Lipid Bilayers; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D015060:1,2-Dipalmitoylphosphatidylcholine; C032166:1,2-hexanoylphosphatidylcholine