title: Cancellous bone osseointegration is enhanced by in vivo loading.
abstract: Biophysical stimuli may be an effective therapy to counteract age-related changes in bone structure that affect the primary stability of implants used in joint replacement or fracture fixation. The influence of controlled mechanical loading on osseointegration was investigated using an in vivo device implanted in the distal lateral femur of 12 male rabbits. Compressive loads (1 MPa, 1 Hz, 50 cycles/day, 4 weeks) were applied to a porous titanium foam implant and the underlying cancellous bone. The contralateral limbs served as nonloaded controls. Backscattered electron imaging indicated that the amount of bone ingrowth was significantly greater in the loaded limb than in the nonloaded control limb, whereas the amount of underlying cancellous periprosthetic bone was similar. No significant difference in the mineral apposition rate of the bone ingrowth or periprosthetic bone was measured in the loaded compared to the control limb. Histological analysis demonstrated newly formed woven bone in direct apposition to the implant coating, with a lack of fibrous tissue at the implant-periprosthetic bone interface in both loaded and nonloaded implants. The lack of fibrous tissue demonstrates that mechanical stimulation using this model significantly enhanced cancellous bone ingrowth without the detrimental effects of micromotion. These results suggest that biophysical therapy should be further investigated to augment current treatments to enhance long-term fixation of orthopedic devices. Additionally, this novel in vivo loading model can be used to further investigate the influence of biophysical stimulation on other tissue engineering approaches requiring bone ingrowth into both metallic and nonmetallic cell-seeded scaffolds.
authors.affiliation: Hospital for Special Surgery, New York, New York, USA.
authors.forename: Bettina M
authors.initials: BM
authors.lastname: Willie
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.forename: Natalie H
authors.initials: NH
authors.lastname: Kelly
authors.forename: Jane
authors.initials: J
authors.lastname: Han
authors.forename: Turya
authors.initials: T
authors.lastname: Nair
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Wright
authors.forename: Marjolein C H
authors.initials: MC
authors.lastname: van der Meulen
authors.forename: Mathias P G
authors.initials: MP
authors.lastname: Bostrom
mesh_terms: D000818:Animals; D001846:Bone Development / Q000502:physiology; D001861:Bone Regeneration / Q000502:physiology; D001842:Bone and Bones / Q000166:cytology* / Q000502:physiology*; D002113:Calcification, Physiologic / Q000502:physiology; D019245:Compressive Strength / Q000502:physiology; D005269:Femur / Q000502:physiology; D008297:Male; D055595:Mechanical Phenomena; D008954:Models, Biological; D016348:Osseointegration / Q000502:physiology*; D019736:Prostheses and Implants; D011817:Rabbits; D016474:Weight-Bearing / Q000502:physiology