title: Analysis of the biosynthesis genes and chemical components of the capsule of Avibacterium paragallinarum.
abstract: The aim of this study was to investigate biosynthesis genes and chemical components of the capsule of Avibacterium paragallinarum. The sequence of a 10-kb region containing the capsule biosynthetic locus of Av. paragallinarum was determined. Two reference strains, i.e., 221 (serovar A) and H18 (serovar C), together with four Taiwanese field strains (all serovar C) were sequenced. The results showed that there are two genotypes (I and II) of the capsule biosynthetic locus in Av. paragallinarum, and the capsule genotype is independent of the serovar. The capsule biosynthetic loci of genotypes I and II consisted of six and five genes, respectively. The genotype I genes encoded proteins that are most similar to proteins from Pasteurella multocida capsule types A and F while the genotype II genes encoded proteins most similar to proteins from P. multocida capsule type D and Escherichia coli K5. The results suggested that genotype I strains contain hyaluronan or chondroitin in the capsule wall while genotype II contain heparosan. Enzymatic digestion of the capsule materials extracted from Av. paragallinarum showed that genotype I strains contained chondroitin while genotype II strains contained heparosan in the capsule. This is the first report on the existence of different genotypes of capsule biosynthesis genes in Av. paragallinarum and the presence of chondroitin and heparosan as chemical components of the capsule of Av. paragallinarum.
authors.affiliation: Graduate Institute of Microbiology and Public Health, National Chung Hsing University, No. 250, Kuokuang Road, Taichung 402, Taiwan.
authors.forename: Jin-Ru
authors.initials: JR
authors.lastname: Wu
authors.forename: Ping-Yi
authors.initials: PY
authors.lastname: Chen
authors.forename: Jui-Hung
authors.initials: JH
authors.lastname: Shien
authors.forename: Ching-Lin
authors.initials: CL
authors.lastname: Shyu
authors.forename: Happy K
authors.initials: HK
authors.lastname: Shieh
authors.forename: Fanny
authors.initials: F
authors.lastname: Chang
authors.forename: Poa-Chun
authors.initials: PC
authors.lastname: Chang
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D016667:Bacterial Capsules / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens / Q000382:microbiology; D002807:Chondroitin / Q000096:biosynthesis / Q000235:genetics; D002874:Chromosome Mapping / Q000662:veterinary; D005798:Genes, Bacterial / Q000235:genetics*; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary; D044134:Haemophilus paragallinarum / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000382:microbiology; D016415:Sequence Alignment / Q000662:veterinary; D012703:Serotyping / Q000662:veterinary
chemical_list: D000942:Antigens, Bacterial; D002807:Chondroitin