title: X-linked insertion/deletion polymorphisms: forensic applications of a 33-markers panel.
abstract: Insertion/deletion (INDEL) polymorphisms are diallelic markers with potential characteristics for use in forensics and biological anthropology, including: the simplicity of laboratory analysis, the possibility of genotyping many markers in a single PCR multiplex reaction, as well as analyzing markers with special inheritance types, such as those linked to the X chromosome (X-INDEL). In this work we developed a laboratory analysis methodology using a 33-INDEL marker panel for the X chromosome in a single PCR multiplex reaction, followed by a capillary electrophoresis run. We employed the panel to genotype a sample of 351 individuals of a mixed population from the Brazilian Amazon. The results demonstrate that the measurement of biostatistical parameters for forensic use in this population is compatible with prior estimates from other populations using current X-STR panels.
authors.affiliation: Laboratório de Genética Humana e Médica, Instituto de Ciências Biológicas, Universidade Federal do Pará, Cidade Universitária Prof. José da Silva Netto. Av. Augusto Corrêa, 01, Belém, PA, Brazil.
authors.forename: Natalle S C
authors.initials: NS
authors.lastname: Freitas
authors.forename: Rafael L
authors.initials: RL
authors.lastname: Resque
authors.forename: Elzemar M
authors.initials: EM
authors.lastname: Ribeiro-Rodrigues
authors.forename: João F
authors.initials: JF
authors.lastname: Guerreiro
authors.forename: Ney P C
authors.initials: NP
authors.lastname: Santos
authors.forename: Andrea
authors.initials: A
authors.lastname: Ribeiro-dos-Santos
authors.forename: Sidney
authors.initials: S
authors.lastname: Santos
mesh_terms: D001938:Brazil; D002874:Chromosome Mapping / Q000295:instrumentation / Q000379:methods*; D041321:Chromosomes, Human, X / Q000235:genetics*; D005260:Female; D053803:Forensic Genetics / Q000295:instrumentation / Q000379:methods*; D005787:Gene Frequency; D050172:Genes, X-Linked / Q000235:genetics*; D005819:Genetic Markers; D005828:Genetics, Population / Q000379:methods*; D005838:Genotype; D006801:Humans; D054643:INDEL Mutation / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D010334:Paternity; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers