title: A protein chip designed to differentiate visually antibodies in chickens which were infected by four different viruses.
abstract: This study aimed to develop a visual protein chip that can differentiate the antibodies induced by avian influenza virus, Newcastle disease virus, infectious bronchitis virus and infectious bursal disease virus, simultaneously. Proteins from the four viruses were purified and spotted onto an aldehyde group-modified glass slide at 2mg/ml. After that, the protein chip was reacted with the corresponding positive serum against these viruses, hybridized with a colloidal gold-labeled secondary antibody and visualized by silver staining. A diagnostic protein chip was constructed to differentiate antibodies of four poultry diseases This protein chip showed good sensitivity compared with traditional methods, and it was more than 400 times as sensitive as the agar gel precipitin methods used to detect avian influenza and infectious bursal disease. The protein chip was used to test known serum samples of the four poultry diseases and field serum samples. The results showed that this method could hybridize specifically with the corresponding antibodies with strong signals and without cross-hybridization. In conclusion, this protein chip can be used to differentiate the antibodies induced by the four avian viruses.
authors.affiliation: Animal Influenza Laboratory of Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, China. wxr@hvri.ac.cn
authors.forename: Xiurong
authors.initials: X
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Shi
authors.forename: Qimeng
authors.initials: Q
authors.lastname: Tao
authors.forename: Hongmei
authors.initials: H
authors.lastname: Bao
authors.forename: Jianan
authors.initials: J
authors.lastname: Wu
authors.forename: Dongdong
authors.initials: D
authors.lastname: Cai
authors.forename: Fumei
authors.initials: F
authors.lastname: Wang
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Zhao
authors.forename: Guobin
authors.initials: G
authors.lastname: Tian
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Li
authors.forename: Chuanling
authors.initials: C
authors.lastname: Qao
authors.forename: Hualan
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000302:isolation & purification; D002645:Chickens; D001351:Infectious bronchitis virus / Q000276:immunology / Q000302:isolation & purification; D007243:Infectious bursal disease virus / Q000276:immunology / Q000302:isolation & purification; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification; D009522:Newcastle disease virus / Q000276:immunology / Q000302:isolation & purification; D011201:Poultry Diseases / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D040081:Protein Array Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000302:isolation & purification; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins