Development of highly sensitive fluorescent probes for detection of intracellular copper(I) in living systems.
We describe here the new copper-selective fluorescent probes FluTPA1 and FluTPA2, in which the tetradentate ligand tris[(2-pyridyl)methyl]amine (TPA) is connected to a reduced form of a fluorescein platform through a benzyl ether linkage. These probes selectively react with copper ions in the presence of submillimolar glutathione and emit intense green fluorescence from the reaction product, O-methylfluorescein. Confocal images of live cells further show that FluTPA2 is membrane-permeable and allows visualization of Cu(I) present in living cells.
Taki
Masayasu
M
Graduate School of Human and Environmental Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan. taki@chem.mbox.media.kyoto-u.ac.jp
Iyoshi
Shohei
S
Ojida
Akio
A
Hamachi
Itaru
I
Yamamoto
Yukio
Y
D002477:Cells / Q000737:chemistry*; D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008744:Methylamines / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
C550941:FluTPA1 compound; C550940:FluTPA2 compound; D005456:Fluorescent Dyes; D008744:Methylamines; D011725:Pyridines; D003300:Copper