Parkinson's disease-associated DJ-1 modulates innate immunity signaling in Caenorhabditis elegans.
DJ-1 is a neuroprotective gene mutated in recessive Parkinson's disease (PD). In addition to direct protective functions in neurons, DJ-1 regulates neuroinflammatory signaling in primary mouse brain astrocytes. To assess the influence of DJ-1 on innate immunity signaling in vivo, we have generated djr-1 knockout Caenorhabditis elegans. When grown on pathogenic gram-negative bacteria, djr-1 (-/-) worms showed stronger phosphorylation of p38 mitogen-activated protein kinase (PMK-1) and hyper-induction of PMK-1 target genes. Thus, PD-associated DJ-1 contributes to regulation of innate immunity.
Cornejo Castro
Elena M
EM
Laboratory of Functional Neurogenetics, Department of Neurodegeneration, Hertie Institute for Clinical Brain Research, German Center for Neurodegenerative Diseases, University of Tübingen, Otfried-Müller-Strasse 27, 72076 Tübingen, Germany.
Waak
Jens
J
Weber
Stephanie S
SS
Fiesel
Fabienne C
FC
Oberhettinger
Philipp
P
Schütz
Monika
M
Autenrieth
Ingo B
IB
Springer
Wolfdieter
W
Kahle
Philipp J
PJ
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D055786:Gene Knockout Techniques; D007113:Immunity, Innate / Q000235:genetics*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D010300:Parkinson Disease / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation / Q000502:physiology; D015398:Signal Transduction / Q000276:immunology*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; D009419:Nerve Tissue Proteins; D029961:Zebrafish Proteins; C515379:park7 protein, zebrafish; D020928:Mitogen-Activated Protein Kinases; C463460:Pmk-1 protein, C elegans