Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3.
The intranuclear disposition of plasmid DNA is extremely important for transgene expression. Exogenous histones have been used as carriers of plasmid DNA in histone-mediated gene delivery. In this study, the effects of exogenous histone H3 complexed with plasmid DNA on transgene expression efficiency were examined. The plasmid-histone complexes in various ratios were transfected into HeLa cells by osmotic pressure. Histone H3 suppressed transgene expression in the nucleus in a dose-dependent manner. Our results suggest that the histone-mediated gene delivery is unlikely to be useful, from the viewpoint of the intranuclear disposition.
Kamiya
Hiroyuki
H
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo, Japan. hirokam@pharm.hokudai.ac.jp
Goto
Hitomi
H
Kanda
Genki
G
Yamada
Yuma
Y
Harashima
Hideyoshi
H
D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006657:Histones / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008156:Luciferases / Q000235:genetics; D009997:Osmotic Pressure; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D019076:Transgenes
D004337:Drug Carriers; D006657:Histones; D004247:DNA; D008156:Luciferases