Expression and localization of Bombyx mori V-ATPase 16 kDa subunit c.
V-ATPase plays a central role in lepidopteran midgut ion transport physiology, and lepidopteran midgut turned out to be a model tissue for the study of V-ATPase. In the present study, the 5'-RACE method is used to obtain the 5'-UTR of V-ATPase c subunit gene from Bombyx mori. Sequence analysis of the promoter region and 3'-UTR of V-ATPase c subunit gene revealed that the transcription of the V-ATPase c subunit gene may be regulated by multi-ways. RT-PCR analysis showed that B. mori V-ATPase c subunit mRNA expresses in the whole developmental stages of B. mori. We also constructed a transient vector to determine the subcellular localization of the B. mori V-ATPase c subunit, and the result demonstrated that it is located in the membrane and some specific regions of BmN cells. Real-time PCR analysis further indicated that the c subunit mRNA expression was upregulated significantly at 24 and 72 h in the midguts of resistant B. mori larvae after being inoculated with B. mori nucleopolyhedrovirus, suggesting that it may be related to the immune response against virus infection.
Lü
Peng
P
Institute of Life Sciences, Jiangsu University, No. 301 Xuefu Road, Zhenjiang 212013, P. R. China.
Chen
Keping
K
Yao
Qin
Q
Gao
Lu
L
Pan
Ye
Y
He
Yuanqing
Y
Huang
Guoping
G
Wang
Lin
L
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D012831:Bombyx / Q000201:enzymology*; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D005822:Genetic Vectors; D007814:Larva / Q000201:enzymology; D011401:Promoter Regions, Genetic; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
D017931:DNA Primers; D021122:Protein Subunits; D012333:RNA, Messenger; D000251:Adenosine Triphosphatases