title: [Determination of streptomycin in honey determined by hydrophilic interaction chromatography combined with tandem mass spectrometry].
abstract: OBJECTIVE
  To establish a method for the determination of streptomycin in honey by determined hydrophilic interaction chromatography combined with tandem mass spectrometry (HILIC-MS/MS).
  
  
  METHODS
  A hilic column was used, and the mobile phase was consisted of acetonitrile (5 mmol/L ammonium acetate and 0.1% formic acid, 60:40). Samples were extracted by the cation-exchange SPE cartridge. The extraction samples were then identified and quantitated by HILIC-ESI-MS/MS.
  
  
  RESULTS
  Linear calibration curves were obtained at the concentration ranges from 10. 5 microg/kg to 105 microg/kg (the correlation coefficients were above 0.99). The limit of detection was 1.05 microg/kg. The average recoveries for streptomycin spiked in honey ranged from 71.3 to 82.5% and their relative standard were between 3.4% and 8.2%.
  
  
  CONCLUSIONS
  The method for determination of streptomycin in honey could be simple, sensitive and accurate.
authors.affiliation: Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. yun-niu@163.com
authors.forename: Lingyun
authors.initials: L
authors.lastname: Chen
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Zhou
authors.forename: Ande
authors.initials: A
authors.lastname: Ma
mesh_terms: D002845:Chromatography / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D057927:Hydrophobic and Hydrophilic Interactions; D013307:Streptomycin / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D013307:Streptomycin