[The effect of the activation of cannabinoid receptor on the proliferation and apoptosis of hepatoma HepG2 cells].
OBJECTIVE
To study the effect of the activation of cannabinoid receptor on the proliferation and apoptosis of hepatoma HepG2 cells.


METHODS
HepG2 cells were treated with different doses of the activator of cannabinoid receptor-WIN-55, 212-2(WIN), MTT was used to detect the effects of WIN on HepG2 cells; Flow cytometry (FCM) was used to examine the effects of WIN on the apoptosis of HepG2 cells. Caspase 3 and c-myc proteins were determined by Western blot assay, and caspase 3, 8, 9 activity was measured using a colorimetric method.


RESULTS
WIN could inhibit HepG2 cell proliferation and induce cell apoptosis dose-dependently. WIN increased caspase 3 protein levels and caspase 3, 8, 9 activity.


CONCLUSIONS
WIN can induce apoptosis, the effect might be associated with up-regulating expression of caspases protein and down-regulating expression of c-myc protein.
Wang
Ying
Y
Department of Biochemistry, Guangzhou Medical College, Guangzhou 510182, China. vio402let@sohu.com
Zhou
Yu-ting
YT
Zhao
Qing
Q
D017209:Apoptosis / Q000187:drug effects*; D048588:Benzoxazines / Q000494:pharmacology; D063386:Cannabinoid Receptor Agonists; D020169:Caspases / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D009025:Morpholines / Q000494:pharmacology; D009281:Naphthalenes / Q000494:pharmacology; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism; D043885:Receptor, Cannabinoid, CB2 / Q000378:metabolism; D043882:Receptors, Cannabinoid / Q000378:metabolism*
D048588:Benzoxazines; D063386:Cannabinoid Receptor Agonists; D009025:Morpholines; D009281:Naphthalenes; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2; D043882:Receptors, Cannabinoid; C070417:(3R)-((2,3-dihydro-5-methyl-3-((4-morpholinyl)methyl)pyrrolo-(1,2,3-de)-1,4-benzoxazin-6-yl)(1-naphthalenyl))methanone; D020169:Caspases