Osseointegration of anodized titanium implants coated with poly(lactide-co-glycolide)/basic fibroblast growth factor by electrospray.
OBJECTIVE
The purpose of this study was to evaluate the effect of basic fibroblast growth factor (bFGF) in combination with a biodegradable polymer coated onto titanium implants. The implants were inserted in rabbit tibiae, and bone growth was examined by histomorphometric analysis.


METHODS
Forty-eight threaded anodized implants were inserted into the tibiae of 12 rabbits. After 12 weeks of healing, an evaluation was made by light microscopy and computerized image analysis. A total of 56 anodized titanium implants were prepared as follows: group 1 was anodized under 300 V; group 2 was anodized then coated with 0.02 mL poly(lactide-co-glycolide) (PLGA) nanoparticles; group 3 was anodized then coated with 0.02 mL PLGA/bFGF nanoparticles (10 ng bFGF); and group 4 was anodized then coated with 0.2 mL PLGA/bFGF nanoparticles (100 ng bFGF).


RESULTS
Nanosized particles were observed on the coated implant surfaces. The mean diameter of the nanoparticles was 268.6 +/- 93.2 nm. The mean bone-to-implant contact (BIC) percentage in group 4 (44.7%) was significantly greater than that seen in groups 1 (31.4%) and 2 (33.6%) (P < .05).


CONCLUSIONS
The results of this preliminary study show that coating a titanium implant with PLGA incorporated with bFGF by electrospray may stimulate bone formation adjacent to the surface of an implant inserted in bone.
Lee
Su Young
SY
Department of Prosthodontics and Dental Research Institute, College of Dentistry Seoul National University, Seoul, South Africa. young21c@snu.ac.kr
Koak
Jai Young
JY
Heo
Seong Joo
SJ
Kim
Seong Kyun
SK
Lee
Seung Jin
SJ
Nam
Seung Yeon
SY
D020341:Absorbable Implants; D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D015921:Dental Implants; D003764:Dental Materials / Q000737:chemistry*; D017267:Dental Prosthesis Design; D055664:Electrochemical Techniques; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry; D016348:Osseointegration / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D011817:Rabbits; D013499:Surface Properties; D013977:Tibia / Q000601:surgery; D014025:Titanium / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D003764:Dental Materials; D016222:Fibroblast Growth Factor 2; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D014025:Titanium