[Effect of BuYangHuanWu recipe on cerebral microcirculation in gerbils with ischemia-reperfusion].
OBJECTIVE
To explore the protective mechanism of BuYangHuanWu recipe on neurofunction in gerbils with cerebral ischemia- reperfusion.


METHODS
Gerbils (n = 48) were divided randomly into three groups: animal model group, BuYangHuanWu recipe group, and sham control group. The animal model of cerebral ischemia was established using bilateral common carotid artery occlusion followed by unclamp 45 min after occlusion. The microcirculation was observed with a Laser Doppler. The density of microvascular was measured using Tannic acid ferric chloride mordant dyeing. The BBB (blood brain barrier) permeability was assessed using evan's blue (EB) dye. The water content in brain tissues was tested with wet and dry method. The learning and memory function test was performed with a 4-PTT dry path maze.


RESULTS
Compared with the animal model group, BuYangHuanWu recipe increased blood flow in the hippocampal region at 1 and 5 min after occlusion, inhibited hypoperfusion at 15 min after reperfusion, increased blood flow at 30, 60 and 120 min after reperfusion. Meanwhile, BuYangHuanWu recipe inhibited the increase of BBB permeability and water content in brain tissues after reperfusion (P < 0.05). BuYangHuanWu recipe also improved the scores of learning and memory function of the gerbils.


CONCLUSIONS
BuYangHuanWu recipe protects the neurofunction in gerbils with ischemia-reperfusion through modulating cerebral microcirculation damages.
Zhao
Ya-Ning
YN
Department of Neurosurgery, North China Coal Medical College,Tangshan 063000, China. Histology Embryology, Chengde Medical College, Chengde 064000, China.
Wu
Xiao-Guang
XG
Li
Jian-Min
JM
Chen
Chang-Xiang
CX
Rao
Ying-Zhen
YZ
Li
Shu-Xing
SX
D000818:Animals; D001921:Brain / Q000098:blood supply*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005849:Gerbillinae; D020925:Hypoxia-Ischemia, Brain / Q000188:drug therapy* / Q000503:physiopathology; D007858:Learning / Q000187:drug effects; D008297:Male; D008568:Memory / Q000187:drug effects; D008833:Microcirculation / Q000187:drug effects; D008517:Phytotherapy; D011897:Random Allocation; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000503:physiopathology
D004365:Drugs, Chinese Herbal; C460102:buyang huanwu