title: Ultrasonic enhancement of waste activated sludge hydrolysis and volatile fatty acids accumulation at pH 10.0.
abstract: Volatile fatty acids (VFA), the preferred carbon source for biological nutrients removal, can be produced by waste activated sludge (WAS) anaerobic fermentation. However, because the rate of VFA accumulation is limited by that of WAS hydrolysis and VFA is always consumed by methanogens at acidic or neutral pHs, the ultrasonic pretreatment which can accelerate the rate of WAS hydrolysis, and alkaline adjustment which can inhibit the activities of methanogens, were, therefore, used to improve WAS hydrolysis and VFA accumulation in this study. Experiment results showed that the combination of ultrasonic pretreatment and alkaline adjustment caused significant enhancements of WAS hydrolysis and VFA accumulation. The study of ultrasonic energy density effect revealed that energy density influenced not only the total VFA accumulation but also the percentage of individual VFA. The maximal VFA accumulation (3109.8mg COD/L) occurred at ultrasonic energy density of 1.0kW/L and fermentation time of 72h, which was more than two times that without ultrasonic treatment (1275.0mg COD/L). The analysis of VFA composition showed that the percentage of acetic acid ranked the first (more than 40%) and those of iso-valeric and propionic acids located at the second and third places, respectively. Thus, the suitable ultrasonic conditions combined with alkaline adjustment for VFA accumulation from WAS were ultrasonic energy density of 1.0kW/L and fermentation time of 72h. Also, the key enzymes related to VFA formation exhibited the highest activities at ultrasonic energy density of 1.0kW/L, which resulted in the greatest VFA production during WAS fermentation at pH 10.0.
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai, 200092, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Yan
authors.forename: Leiyu
authors.initials: L
authors.lastname: Feng
authors.forename: Chaojie
authors.initials: C
authors.lastname: Zhang
authors.forename: Christelle
authors.initials: C
authors.lastname: Wisniewski
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000143:Acids / Q000032:analysis; D000693:Anaerobiosis; D002244:Carbon / Q000032:analysis; D004798:Enzymes / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000032:analysis* / Q000737:chemistry; D005285:Fermentation; D019220:High-Energy Shock Waves; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010758:Phosphorus / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D012722:Sewage / Q000737:chemistry*; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D000143:Acids; D004798:Enzymes; D005232:Fatty Acids, Volatile; D000644:Quaternary Ammonium Compounds; D012722:Sewage; D014874:Water Pollutants, Chemical; D010758:Phosphorus; D002244:Carbon