title: Diversity of Crenarchaeota in terrestrial hot springs in Tengchong, China.
abstract: Diversity of Crenarchaeota was investigated in eight terrestrial hot springs (pH 2.8-7.7; temperature 44-96 degrees C) located in Tengchong, China, using 16S rRNA gene phylogenetic analysis. A total of 826 crenarchaeotal clones were sequenced and a total of 47 operational taxonomic units (OTUs) were identified. Most (93%) of the identified OTUs were closely related (89-99%) to those retrieved from hot springs and other thermal environments. Our data showed that temperature may predominate over pH in affecting crenarchaeotal diversity in Tengchong hot springs. Crenarchaeotal diversity in moderate-temperature (59-77 degrees C) hot springs was the highest, indicating that the moderately hot-temperature springs may provide optimal conditions for speciation of Crenarchaeota.
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, China.
authors.forename: Zhao-Qi
authors.initials: ZQ
authors.lastname: Song
authors.forename: Jing-Quan
authors.initials: JQ
authors.lastname: Chen
authors.forename: Hong-Chen
authors.initials: HC
authors.lastname: Jiang
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Tang
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Zhi
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Zhang
authors.forename: Chuan-Lun L
authors.initials: CL
authors.lastname: Zhang
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D002681:China; D003001:Cloning, Molecular; D019615:Crenarchaeota / Q000235:genetics*; D019641:DNA, Archaeal / Q000235:genetics*; D015723:Gene Library; D014644:Genetic Variation; D005844:Geology; D045482:Hot Springs; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D015233:Models, Statistical; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S