[The expression of Basigin mRNA in early ischemic myocardium and non-ischemic myocardium].
OBJECTIVE
To investigate the expression of Basigin mRNA in early ischemic myocardium (EIM) and non-ischemic myocardium (NIM) in rat.


METHODS
Real-time polymerase chain reaction (PCR) technique was applied for detecting Basigin mRNA and beta-actin expression in EIM and NIM at 0 min, 15 min, 30 min, and 60 min.


RESULTS
No significant differences of the Basigin mRNA expression in EIM and NIM between 0 min and sham operation (SO) or non-operation group (P > 0.05) were observed. Basigin mRNA expression in EIM significantly decreased in 15 min, 30 min, and 60 min group compared with that of 0 min group, respectively (P < 0.001). However, we failed to find any significant difference in NIM and NO from 0 min to 60 min. Basigin mRNA expression in EIM decreased significantly compared with that of in NIM (P < 0.05) after myocardial ischemia for 30 min.


CONCLUSIONS
Basigin was involved in myocardium pathophysiology process after myocardial ischemia for 30 min, indicating that Basigin may be identified as a predictor of early myocardial ischemia in forensic medicine.
Liu
Yun
Y
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
Gao
Lin-Bo
LB
Liang
Wei-Bo
WB
Pan
Xin-Min
XM
Wang
Yan-Yun
YY
Xue
Hui
H
Chen
Tian-Yi
TY
Zhang
Lu-Shun
LS
Zhu
Yi
Y
Zhang
Lin
L
D000818:Animals; D051926:Basigin / Q000235:genetics / Q000378:metabolism*; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism*; D005554:Forensic Medicine; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D001798:Blood Proteins; C079432:Bsg protein, rat; D012333:RNA, Messenger; D051926:Basigin