title: Thermoresponsive polymer brush surfaces with hydrophobic groups for all-aqueous chromatography.
abstract: For developing thermoresponsive chromatographic matrices with a strong hydrophobicity, poly(N-isopropylacrylamide-co-n-butyl methacrylate) (poly(IPAAm-co-BMA)) brush grafted silica beads were prepared through a surface-initiated atom transfer radical polymerization (ATRP) with a CuCl/CuCl(2)/Me(6)TREN catalytic system in 2-propanol at 25 degrees C for 16 h. The prepared beads were characterized by chromatographic analysis. Chromatograms of the benzoic-acid family and phenol as model analytes were obtained with high-resolution peaks because of their strong hydrophobic interactions to the densely grafted hydrophobized copolymers on the beads. Retention times of the analytes increased with the increase in BMA composition ratio. Dehydration of grafted copolymer with large BMA composition was performed at low temperature. These results indicated that the copolymer-brush-grafted surface prepared by ATRP was an effective tool for separating hydrophilic analytes at low temperature through modulating the strong hydrophobic interaction.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo 162-8666, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Nagase
authors.forename: Mio
authors.initials: M
authors.lastname: Kumazaki
authors.forename: Hideko
authors.initials: H
authors.lastname: Kanazawa
authors.forename: Jun
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kikuchi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kikuci
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Akiyama
authors.forename: Masahiko
authors.initials: M
authors.lastname: Annaka
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D019817:Benzoic Acid / Q000737:chemistry; D002384:Catalysis; D002845:Chromatography / Q000379:methods*; D006358:Hot Temperature; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D008956:Models, Chemical; D019800:Phenol / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D011108:Polymers; D014867:Water; D019800:Phenol; D012822:Silicon Dioxide; D019817:Benzoic Acid