Genetic characterisation of uninucleated cyst-producing Entamoeba spp. from ruminants.
Six ssrRNA gene sequences were obtained by PCR amplification of DNA from uninucleated Entamoeba cysts isolated from fresh faeces of sheep, cows, a roe deer and a reindeer. Phylogenetic analysis using sequences of non-, uni-, quadri- and octonucleate cyst-producing Entamoeba spp. for comparison showed that all six isolates formed a separate clade nested within the clade of quadrinucleate cyst producers. The data indicate that Entamoeba bovis can be isolated from ruminant hosts other than cattle, and we suggest that organisms clustering with the sheep and cattle isolates analysed in the present study be named E. bovis.
Stensvold
C Rune
CR
Laboratory of Parasitology, Statens Serum Institut, Copenhagen, Denmark. run@ssi.dk
Lebbad
Marianne
M
Clark
C Graham
CG
D000818:Animals; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004747:Entamoeba / Q000145:classification* / Q000166:cytology / Q000235:genetics* / Q000302:isolation & purification; D004749:Entamoebiasis / Q000469:parasitology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012418:Ruminants / Q000469:parasitology*; D017422:Sequence Analysis, DNA; D033761:Spores, Protozoan / Q000166:cytology
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S