[Expression of CREB/Bcl-2 in bone marrow mononuclear cells of children with acute leukemia].
OBJECTIVE
To study the expression and role of cyclic-AMP response binding protein (CREB) and Bcl-2 in children with acute leukemia.


METHODS
Ninety-two children with acute leukemia (leukemia group) and 30 children with non-hematologic malignancies (control group) were enrolled. The mRNA and protein expression of CREB and Bcl-2 in bone marrow mononuclear cells were measured by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot.


RESULTS
The mRNA and protein expression of CREB and Bcl-2 in the leukemia group was significantly higher than that in the control group (p<0.01). There were no significant differences in the expression of CREB and Bcl-2 between acute lymphoblastic leukemia and acute myeloid leukemia subgroups. At the initial diagnosis, the mRNA and protein expression of CREB and Bcl-2 in children with extramedullary infiltration was higher than that in children without (p<0.05). In the leukemia group, the mRNA and protein expression of CREB and Bcl-2 in the complete remission subgroup was significantly lower than that in the non-complete remission subgroup (p<0.01). High mRNA expression of CREB and Bcl-2 in the leukemia group was positively correlated with peripheral blood leucocyte counts (r=0.62, 0.71 respectively, p<0.05). There was a positive correlation between mRNA and protein expression of CREB and Bcl-2 (r=0.75, 0.68 respectively; p<0.05).


CONCLUSIONS
The expression of CREB and Bcl-2 may be correlated with the pathogenesis and clinical prognosis of childhood leukemia, however, their expression may not be associated with the classification of acute leukemia.
Wen
Chuan
C
Department of Pediatrics, Second Xiangya Hospital, Central South University, Changsha 410011, China. wc740317@tom.com
Ma
Fu-Tian
FT
Wan
Wu-Qing
WQ
D000208:Acute Disease; D000293:Adolescent; D001854:Bone Marrow Cells / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D017362:Cyclic AMP Response Element-Binding Protein / Q000032:analysis / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D007938:Leukemia / Q000378:metabolism*; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis
C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger