title: Detection of RTP801, a gene that is differentially expressed in irradiated HeLa cells.
abstract: OBJECTIVE
  To quantify the effects of irradiation on the expression levels of a differentially expressed gene, RTP801, in HeLa cells.
  
  
  METHODS
  Total RNA was isolated from irradiated and non-irradiated HeLa cells. A subtraction library was constructed, from which 88 random clones were screened. The expression patterns of one clone, detected by reverse Northern blotting, were quantified by real time RT-PCR, using CYBR green I dye.
  
  
  RESULTS
  RTP801, a hypoxia-inducible factor-I-responsive gene, was identified as a differentially expressed gene in HeLa cells exposed to X-ray. Real time RT-PCR showed that the mRNA levels of RTP801 were greatly diminished by radiation.
  
  
  CONCLUSIONS
  These results suggest that down-regulation of hypoxia-inducible factor-I-responsive genes, such as RTP801, in irradiated HeLa cells may result in reductions in the radiotherapy resistance of tumor cells.
authors.affiliation: Department of Dermatology, College of Medicine, Chungnam National University, Daejeon, Korea.
authors.forename: Young-Sook
authors.initials: YS
authors.lastname: Lee
authors.forename: Moon-June
authors.initials: MJ
authors.lastname: Cho
authors.forename: Jeung Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Woong-Hee
authors.initials: WH
authors.lastname: Lee
authors.forename: Jun-Sang
authors.initials: JS
authors.lastname: Kim