title: Soft X-ray diffraction microscopy of a frozen hydrated yeast cell.
abstract: We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below -170 degrees C, artifacts due to dehydration, ice crystallization, and radiation damage are greatly reduced. In this example, coherent diffraction data using 520 eV x rays were recorded and reconstructed to reveal a budding yeast cell at a resolution better than 25 nm. This demonstration represents an important step towards high resolution imaging of cells in their natural, hydrated state, without limitations imposed by x-ray optics.
authors.affiliation: Department of Physics & Astronomy, Stony Brook University, Stony Brook, New York 11794-3800, USA.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Huang
authors.forename: Johanna
authors.initials: J
authors.lastname: Nelson
authors.forename: Janos
authors.initials: J
authors.lastname: Kirz
authors.forename: Enju
authors.initials: E
authors.lastname: Lima
authors.forename: Stefano
authors.initials: S
authors.lastname: Marchesini
authors.forename: Huijie
authors.initials: H
authors.lastname: Miao
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Neiman
authors.forename: David
authors.initials: D
authors.lastname: Shapiro
authors.forename: Jan
authors.initials: J
authors.lastname: Steinbrener
authors.forename: Andrew
authors.initials: A
authors.lastname: Stewart
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Turner
authors.forename: Chris
authors.initials: C
authors.lastname: Jacobsen
mesh_terms: D005615:Freezing; D008853:Microscopy / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D014867:Water / Q000378:metabolism*; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D014867:Water