Production of recombinant human granulocyte macrophage colony-stimulating factor from silkworm Bombyx mori Bm5 cells.
Using silkworm Bombyx mori Bm5 cells, we established a stable cell line expressing the human granulocyte macrophage colony-stimulating factor (hGM-CSF), which gets its name from the Bm5-hGM-CSF cell in which the glycoprotein of the hGM-CSF is secreted in the cell culture supernatant (CCS). It was demonstrated that secreted hGM-CSF had in vivo biological activity and the white blood cell (WBC) value increased two times that of the control. We expect to produce useful human recombinant glycoproteins from silkworm cultured cells for a low price and a large quantity.
Jin
Cho-Yi
CY
Department of Anatomy, School of Medicine, Chungnam National University, Taejon 301-747, Korea.
Han
Song-Yi
SY
Kwon
Kisang
K
Yun
Eun Young
EY
Kang
Seok Woo
SW
Goo
Tae Won
TW
Kim
Seung-Whan
SW
Yu
Kweon
K
Kwon
O-Yu
OY
D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002460:Cell Line; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor