The first example of MEEKC-ICP-MS coupling and its application for the analysis of anticancer platinum complexes.
MEEKC is a powerful electrodriven separation technique with many applications in different disciplines, including medicinal chemistry; however, up to now the coupling to highly sensitive and selective MS detectors was limited due to the ion suppressive effect of the commonly used surfactant SDS. Herein, the first example of the coupling of MEEKC to ICP-MS is presented and an MEEKC method for the separation of Pt(II) and Pt(IV) anticancer drugs and drug candidates was developed. Different compositions of microemulsions were evaluated and the data were compared with those collected with standard ultraviolet/visible (UV/vis) spectroscopy detection. The MEEKC-ICP-MS system was found to be more sensitive than MEEKC-UV/vis and the analysis of UV/vis silent compounds is now achievable. The migration behavior of the Pt(II) and Pt(IV) compounds under investigation is correlated to their different chemical structures.
Bytzek
Anna K
AK
Institute of Inorganic Chemistry, University of Vienna, Vienna, Austria.
Reithofer
Michael R
MR
Institute of Inorganic Chemistry, University of Vienna, Vienna, Austria.
Galanski
Markus
M
Institute of Inorganic Chemistry, University of Vienna, Vienna, Austria.
Groessl
Michael
M
Institute of Inorganic Chemistry, University of Vienna, Vienna, Austria.
Keppler
Bernhard K
BK
Institute of Inorganic Chemistry, University of Vienna, Vienna, Austria.
Hartinger
Christian G
CG
Institute of Inorganic Chemistry, University of Vienna, Vienna, Austria.
D000970:Antineoplastic Agents / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry / Q000379:methods*; D009944:Organoplatinum Compounds / Q000737:chemistry*; D000077150:Oxaliplatin; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D012967:Sodium Dodecyl Sulfate