[Identification of asthenozoospermia-associated proteins in human seminal plasma by shotgun proteomic strategy].
OBJECTIVE
To identify asthenozoospermia-associated proteins in seminal plasma by the shotgun proteomic strategy.


METHODS
Six seminal plasma samples were collected by Percoll respectively from healthy fertile and asthenozoospermia volunteers, balanced, mixed, and then the mixture was separated by SDS-PAGE. The proteins in the gel were enzymolyzed, extracted and identified by the shotgun proteomic strategy. The identified proteins with the unique peptide count > or =2 or the unique peptide count=1 but the total count > or =4 were compared between the two groups.


RESULTS
A total of 172 differential proteins were identified, of which, 89 were exclusively from the asthenozoospermia and 83 exclusively from the healthy fertile men. According to the molecular function, these differential proteins were mainly the types of signal transduction and catalytic activity.


CONCLUSIONS
Functionally, 10 of the proteins are particularly important, which include annexin VI isoform 2, isoform 1 of interleukin-6 receptor subunit beta precursor, Mr 400,000 protein, cytosolic dynein heavy chain, alpha-actinin-4, receptor-type tyrosine-protein phosphatase eta precursor, vitamin D-binding protein precursor, protein S100-A11, protein S100-A9 and ANXA4.
Bai
Jie
J
Clinical Laboratory, PLA General Hospital, Beijing 100853, China. jiebai69@tom.com
Fu
Shu-hong
SH
Cai
Li-li
LL
Sun
Ling
L
Cong
Yu-long
YL
D000328:Adult; D053627:Asthenozoospermia / Q000503:physiopathology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D040901:Proteomics; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000302:isolation & purification*; D014809:Vitamin D-Binding Protein / Q000302:isolation & purification
D029589:Seminal Plasma Proteins; D014809:Vitamin D-Binding Protein