title: Differential gene expression between neuropeptide Y expressing neurons of the dorsomedial nucleus of the hypothalamus and the arcuate nucleus: microarray analysis study.
abstract: The Dorsomedial Nucleus of the Hypothalamus (DMH) is known to play important roles in ingestive behavior and body weight homeostasis. The DMH contains neurons expressing Neuropeptide Y (NPY) during specific physiological conditions of hyperphagia and obesity, however, the role of DMH-NPY neurons has yet to be characterized. In contrast to the DMH-NPY neurons, NPY expressing neurons have been best characterized in the Arcuate Nucleus of the Hypothalamus (ARH). The purpose of this study is to characterize the chemical phenotype of DMH-NPY neurons by comparing the gene expression profiles of NPY neurons in the DMH and ARH isolated from postnatal NPY-hrGFP mice by microarray analysis. Twenty genes were differentially expressed in the DMH-NPY neurons compared to the ARH. Among them, there were several transcriptional factors that play important roles in the regulation of energy balance. DMH-NPY neurons expressed Glutamic Acid Decarboxylase (GAD) 65 and 67, suggesting that they may be GABAergic, similar to ARH-NPY neurons. While ARH-NPY neurons expressed leptin receptor (ObRb) and displayed the activation of STAT3 in response to leptin administration, DMH-NPY neurons showed neither. These findings strongly suggest that DMH-NPY neurons could play a distinct role in the control of energy homeostasis and are differentially regulated from ARH-NPY neurons through afferent inputs and transcriptional regulators.
authors.affiliation: Division of Neuroscience, Oregon National Primate Research Center, Oregon Health & Science University, 505 NW 185th Avenue, Beaverton, OR 97006-3499, USA.
authors.forename: Shin
authors.initials: S
authors.lastname: Draper
authors.forename: Melissa
authors.initials: M
authors.lastname: Kirigiti
authors.forename: Maria
authors.initials: M
authors.lastname: Glavas
authors.forename: Bernadette
authors.initials: B
authors.lastname: Grayson
authors.forename: C N Angie
authors.initials: CN
authors.lastname: Chong
authors.forename: Betty
authors.initials: B
authors.lastname: Jiang
authors.forename: M Susan
authors.initials: MS
authors.lastname: Smith
authors.forename: Lori M
authors.initials: LM
authors.lastname: Zeltser
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Grove
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism*; D004302:Dorsomedial Hypothalamic Nucleus / Q000378:metabolism*; D015870:Gene Expression; D020869:Gene Expression Profiling; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020738:Leptin / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D042282:Microdissection; D009474:Neurons / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis; D054411:Receptors, Leptin / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020738:Leptin; D009478:Neuropeptide Y; D012333:RNA, Messenger; D054411:Receptors, Leptin; D005968:Glutamate Decarboxylase