[Relationship between proteoglycans and proliferation of human salivary adencid cystic carcinoma].
OBJECTIVE
To investigate the effect of down-regulated proteoglycans on the proliferation of human salivary adenoid cystic carcinoma (SACC).


METHODS
The short hairpin RNA (shRNA) plasmid silencing human xylosyltransferase-I (XT-I) gene was constructed and named shRNA-WJ3. Adenoid cystic carcinoma cells with high metastatic tendency (ACC-M) were transfected by shRNA-WJ3. The plasmid shRNA-HK not targeting any human gene was transfected into ACC-M cells used as negative control. After 48 h of transfection, the positive cells were screened by G418 to isolate the stable transfected cells. Real-time PCR and Western blotting were used to test the gene silence, and the proteoglycans contents of the cells were detected. The stable cell line silenced XT-I was named ACC-M-WJ3. MTT assay was performed to detect the cell proliferation. The cell cycle was analyzed by flow cytometry.


RESULTS
ShRNA-WJ3 showed powerful RNA interference and gene silence of XT-I. The inhibition rate was 83.70% of mRNA expression and 79.60% of protein expression respectively. The content of proteoglycans in ACC-M-WJ3 was down-regulated by 49.71%-54.59%. The results of MTT assay showed that the cell growth was inhibited significantly. S phrase decreased and G₁-G₀ phrase increased in group ACC-M-WJ3 compared with that of group ACC-M-HK (P < 0.05).


CONCLUSIONS
The down-regulated proteoglycans could inhibit the proliferation of human ACC-M cells.
Shi
Hong
H
Department of Oral Pathology, College of Stomatology, Hebei Medical University, Shijiazhuang 050017, China. Email: wangjiephd@163.com.
Wang
Jie
J
Dong
Fu-Sheng
FS
Wang
Xu
X
Li
He-Xiang
HX
D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D020868:Gene Silencing; D006801:Humans; D010430:Pentosyltransferases / Q000235:genetics / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014162:Transfection; D000097608:UDP Xylose-Protein Xylosyltransferase
D010430:Pentosyltransferases; D011509:Proteoglycans; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000097608:UDP Xylose-Protein Xylosyltransferase