title: The choC gene encoding a putative phospholipid methyltransferase is essential for growth and development in Aspergillus nidulans.
abstract: Phosphatidylcholines (PCs) are a class of major cell membrane phospholipids that participate in many physiological processes. Three genes, choA, choB and choC, have been proposed to function in the endogenous biosynthesis of PC in Aspergillus nidulans. In this study, we characterize the choC gene encoding a putative highly conserved phospholipid methyltransferase. The previously reported choC3 mutant allele results from a mutation leading to the E177K amino acid substitution. The transcript of choC accumulates at high levels during vegetative growth and early asexual developmental phases. The deletion of choC causes severe impairment of vegetative growth, swelling of hyphal tips and the lack of both asexual and sexual development, suggesting the requirement of ChoC and PC in growth and development. Noticeably, supplementation of the mutant with the penultimate precursor of PC N, N-dimethylaminoethanol leads to full recovery of vegetative growth, but incomplete progression of asexual and sexual development, implying differential roles of PC and its intermediates in fungal growth and development. Importantly, while the choC deletion mutant shows reduced vegetative growth and precocious cell death until day 4, it regains hyphal proliferation and cell viability from day 5, indicating the presence of an alternative route for cellular membrane function in A. nidulans.
authors.affiliation: China Agricultural University, Beijing, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Tao
authors.forename: Na
authors.initials: N
authors.lastname: Gao
authors.forename: Sanfeng
authors.initials: S
authors.lastname: Chen
authors.forename: Jae-Hyuk
authors.initials: JH
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001483:Base Sequence; D002470:Cell Survival; D005656:Fungal Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D050941:Phosphatidyl-N-Methylethanolamine N-Methyltransferase / Q000235:genetics* / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D050941:Phosphatidyl-N-Methylethanolamine N-Methyltransferase