title: Crystal structure of yeast FAD synthetase (Fad1) in complex with FAD.
abstract: Flavin adenine dinucleotide (FAD) synthetase is an essential enzyme responsible for the synthesis of FAD by adenylation of riboflavin monophosphate (FMN). We have solved the 1.9 A resolution structure of Fad1, the yeast FAD synthetase, in complex with the FAD product in the active site. The structure of Fad1 shows it to be a member of the PP-ATPase superfamily. Important conformational differences in the two motifs involved in binding the phosphate moieties of FAD compared to the Candida glabrata FMNT ortholog suggests that this loop is dynamic and undergoes substantial conformational changes during its catalytic cycle.
authors.affiliation: Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, CNRS-UMR8619, Université de Paris-Sud, Bât 430, 91405 Orsay Cedex, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Leulliot
authors.forename: Karine
authors.initials: K
authors.lastname: Blondeau
authors.forename: Jenny
authors.initials: J
authors.lastname: Keller
authors.forename: Nathalie
authors.initials: N
authors.lastname: Ulryck
authors.forename: Sophie
authors.initials: S
authors.lastname: Quevillon-Cheruel
authors.forename: Herman
authors.initials: H
authors.lastname: van Tilbeurgh
mesh_terms: D041221:Candida glabrata / Q000737:chemistry / Q000201:enzymology; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D009713:Nucleotidyltransferases / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D005182:Flavin-Adenine Dinucleotide; D009713:Nucleotidyltransferases; C020143:FMN adenylyltransferase