PEGylated Chromatography: Efficient Bioseparation on Silica Monoliths Grafted with Smart Biocompatible Polymers.
Novel oligo(ethylene glycol)-based thermoresponsive stationary phases have been studied for the separation of bioanalytes. Well-defined copolymers of (2-methoxyethoxy)ethyl methacrylate and oligo(ethylene glycol) methacrylate were synthesized by atom-transfer radical polymerization in the presence of an N-succinimidyl-functionalized initiator. The reactive chain ends of these copolymers were then reacted with amino-functionalized silica monoliths. The formed composites were studied as chromatography materials. For instance, it was demonstrated that thermoresponsive oligo(ethylene glycol)-based stationary phases allow rapid, efficient separation of steroid and protein mixtures in pure water under isocratic high-performance liquid chromatographic elution.
Tan
Irene
I
Colloid Chemistry Department, Max-Planck Institute of Colloids and Interfaces, Scientific Campus Golm, 14476 Potsdam, Germany.
Zarafshani
Zoya
Z
Lutz
Jean-François
JF
Titirici
Maria-Magdalena
MM
D000180:Acrylic Resins / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D002621:Chemistry / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003348:Cortisone / Q000737:chemistry; D008422:Materials Testing; D008956:Models, Chemical; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
D000180:Acrylic Resins; D001672:Biocompatible Materials; D011108:Polymers; C052970:poly-N-isopropylacrylamide; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D003348:Cortisone