Hydrophobic fractions from Strongyloides venezuelensis for use in the human immunodiagnosis of strongyloidiasis.
The objective of the present research was to evaluate detergent and aqueous phases of total saline (TS) and alkaline extracts of Strongyloides venezuelensis for human strongyloidiasis immunodiagnosis. Total extracts and detergent and aqueous antigenic fractions were separated using Triton X-114 and were examined by enzyme-linked immunosorbent assay (ELISA) and immunoblotting (IB) tests to detect immunoglobulin G (IgG). Serum samples were obtained from 120 individuals: 40 strongyloidiasis patients (group I), 40 patients with other parasitic diseases (group II), and 40 apparently healthy individuals (group III). Each extract provided a different profile of antigenic components as recognized by IgG in IB. The detergent fraction of the TS extract demonstrated the highest sensitivity and specificity for ELISA and IB. The results indicated that the detergent saline fraction, purified from S. venezuelensis, furnished the most valid results for the strongyloidiasis immunodiagnosis and could be employed as an alternative antigen and as a useful source of specific polypeptides.
Feliciano
Nágilla Daliane
ND
Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, 38400-902, Uberlândia, Minas Gerais, Brazil.
Gonzaga
Henrique Tomaz
HT
Gonçalves-Pires
Maria do Rosário Fátima
Mdo R
Gonçalves
Ana Lúcia Ribeiro
AL
Rodrigues
Rosangela Maria
RM
Ueta
Marlene Tiduko
MT
Costa-Cruz
Julia Maria
JM
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D007159:Immunologic Tests / Q000379:methods; D010274:Parasitology / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013321:Strongyloides / Q000737:chemistry* / Q000276:immunology*; D013322:Strongyloidiasis / Q000175:diagnosis*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G