title: Intratumoral administration of anti-KITENIN shRNA-loaded PEI-alt-PEG nanoparticles suppressed colon carcinoma established subcutaneously in mice.
abstract: Biodegradable gene carrier, termed as PEI-alt-PEG, has been synthesized based on Michael addition reaction between lower Mw PEI and poly(ethylene glycol) (PEG) diacrylate and tested its potential of anti-metastatic cancer gene therapy by using anti-KITENIN short hairpin RNA. KITENIN is known to promote invasion of mouse colon adenocarcinoma in vivo. Intratumoral administration of anti-KITENIN shRNA-loaded PEI-alt-PEG nanoparticles has shown suppressed proliferlation and enhanced apoptosis signal in tumor compared to commercial available liposome, leading to delayed tumor growth.
authors.affiliation: Department of Biomedical Sciences, Chonnam National University Medical School, The Brain Korea 21 Project, Centre for Biomedical Human Resources at Chonnam National University, Gwangju 501-746, South Korea.
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Park
authors.forename: Kyung Keun
authors.initials: KK
authors.lastname: Kim
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Cho
authors.forename: Woo-Kyun
authors.initials: WK
authors.lastname: Bae
authors.forename: Dhananjay
authors.initials: D
authors.lastname: Jere
authors.forename: Chong-Su
authors.initials: CS
authors.lastname: Cho
authors.forename: Ik-Joo
authors.initials: IJ
authors.lastname: Chung
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000628:therapy*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D020868:Gene Silencing; D015316:Genetic Therapy / Q000379:methods*; D007279:Injections, Subcutaneous; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008968:Molecular Conformation; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D050997:Nanomedicine / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000031:analogs & derivatives / Q000737:chemistry; D012313:RNA / Q000008:administration & dosage* / Q000235:genetics; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014162:Transfection / Q000379:methods
chemical_list: D002352:Carrier Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D053769:Nanocapsules; C488287:VANGL1 protein, human; C544016:poly(ethylene glycol)-co-poly(ethyleneimine); C009495:titanium dioxide; D011092:Polyethylene Glycols; D012313:RNA; D011094:Polyethyleneimine; D014025:Titanium