title: Magnesium and posthypoxic changes of nitrergic population in rat hippocampus.
abstract: We used NADPH-diaphorase staining to study effects of magnesium pre-treatment during long-lasting hypoxia on the brain structure of rats. NADPH-diaphorase is an enzyme co-localized in neurons with NO-synthase that is responsible for NO synthesis. NO participates in hypoxic-ischaemic injury of the brain. Hypoxia was induced in consecutive days from the 2nd till the 11th day of postnatal life in a hypobaric chamber (for 8 hours per day). Magnesium was administered before each hypoxia exposition. At the age of 12 days, the animals were transcardially perfused with 4% buffered neutral paraformaldehyde under the deep thiopental anaesthesia. Cryostat sections were stained to identify NADPH-diaphorase positive neurons that were then quantified in five hippocampal regions. In comparison to the control animals, intermittent hypoxia brought about higher density of NADPH-diaphorase positive neurons in all studied areas of the hippocampal structure: in CA1 and CA3 areas of the hippocampus and in hilus, in the dorsal and ventral blades of the dentate gyrus. Magnesium pre-treatment during hypoxia reduced number of NADPH-diaphorase positive neurons in all studied areas.
authors.affiliation: Charles University in Prague, Institute of Physiology, Prague, Czech Republic. katerina.jandova@lf1.cuni.cz
authors.forename: K
authors.initials: K
authors.lastname: Jandová
authors.forename: V
authors.initials: V
authors.lastname: Riljak
authors.forename: D
authors.initials: D
authors.lastname: Maresová
authors.forename: M
authors.initials: M
authors.lastname: Langmeier
authors.forename: J
authors.initials: J
authors.lastname: Pokorný
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009569:Nitric Oxide; D009252:NADPH Dehydrogenase; D008274:Magnesium