Meat quality and carcass traits in relation to HGD-BstXI and HGD-HaeIII PCR-RFLP polymorphism in Chinese red cattle.
We investigated the effect of homogentisate 1, 2 dioxygenase (HGD) gene on meat quality and carcass traits in 287 Chinese red cattle. The PCR-SSCP method was used to identify polymorphism of the HGD gene in the exon 1 and intron 1. Two polymorphisms were detected in intron 1 and two restriction sites for endonuclease HGD-BstXI and HGD-HaeIII have also been found. The HGD-BstXI genotypes showed significant effects on cooking loss, drip loss, net meat weight, carcass weight, and eye muscle area (P<0.05). The HGD-HaeIII genotypes significant affected cooking loss, muscle fibre diameter, shear force, drip loss, and carcass yield ratio (P<0.05). Moreover, we found significant effects of diplotypes on cooking loss, muscle fibre diameter, shear force, drip loss, net meat weight, carcass weight, and eye muscle area (P<0.05).
Zhou
G L
GL
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, People's Republic of China; College of Life Science, Liaocheng University, Liaocheng 252059, People's Republic of China.
Cao
Y
Y
Li
M
M
Zhang
L C
LC
Yu
Y S
YS
Jin
H G
HG
D000818:Animals; D001823:Body Composition / Q000235:genetics / Q000502:physiology*; D002417:Cattle / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D050560:Homogentisate 1,2-Dioxygenase / Q000235:genetics*; D008460:Meat / Q000592:standards*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D050560:Homogentisate 1,2-Dioxygenase; C047564:CCANNNNNNTGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases