[Production of ligninase by co-fermentation of Coprinus comatus and Trichoderma reesei].
In order to enhance the utilization efficiency, reduce the environmental pollution of traditional chemical treatment and the agriculture waste incineration; we studied the ligninase production by Coprinus comatus, Aspergillus niger and Trichoderma reesei through the plate screening. The results showed that C. comatus mixed culture with T. reesei have a good compatibility and higher yields of Laccase. On the basis of this pre-experiment, we studied the optimal conditions of mixed culture for enzyme production. Under the optimal conditions: the inoculation proportion of C. comatus and T. reesei (5:2), the interval of time (12 h), the temperature 260C, the shake rotation speed 150 r/min, fermented for 3 days, the Laccase activity reached 3267.1 U/mL, increased by 106% contrasted with single culture of C. commatus.
Ge
Chunmei
C
Department of Biology & Environment Engineering, Hefei University, Hefei 230022, China.
Xu
Juanjuan
J
Sun
Qinying
Q
Zhang
Jie
J
Cai
Jingmin
J
Pan
Renrui
R
D003302:Coprinus / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods; D005285:Fermentation; D010105:Oxygenases / Q000096:biosynthesis*; D018547:Plant Stems / Q000378:metabolism; D014242:Trichoderma / Q000378:metabolism*; D003313:Zea mays / Q000378:metabolism
D010105:Oxygenases; C044391:ligninase