[Genetic diversity of Changium smyrnioides based on SRAP].
OBJECTIVE
To study the genetic diversity of Changium smyrnioides and give a reference for utilization of the germplasm.


METHODS
Ten different populations of Ch. smyrnioides were analyzed by the approach of sequence-related amplified polymorphism (SRAP). Genetic similarity coefficient was calculated, and systematic relationships were constructed based on the UPGMA method.


RESULTS
Seventeen primer pairs were selected from 160. A total of 363 bands were scored, 314 bands of them were polymorphic and the average was 18.47 polymorphic bands per primer pair, which were up to 86.50% polymorphic ratio. The results indicated that there was abundant genetic diversity among the tested materials. Genetic similarity coefficient was ranged from 0.4959 to 0.8182. Cluster analysis showed that ten different populations of Ch. smyrnioides could be distinguished into two groups.


CONCLUSIONS
High level genetic diversity was in different populations of Ch. smyrnioides, and genetic relationship was correlative to geographic position.
Wang
Changlin
C
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Guo
Qiaosheng
Q
Wu
Yumei
Y
D019661:Apiaceae / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010802:Phylogeny; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction