title: Spontaneous skin damage and delayed wound healing in SOD1-deficient mice.
abstract: Superoxide dismutase 1 (SOD1) is an important antioxidative enzyme that protects skin from oxidative stress. SOD1 (-/-) mice with a genetic background of b129Sv mice showed facial skin damage after 15 weeks of age. Eyelid swelling occurred as the initial symptom and caused impairment by triggering self-scratching. The period required for wound healing in the back was markedly delayed in 20-week SOD1 (-/-) mice. Oxidative stress markers, 4-hydroxynonenal and thiobarbituric acid-reactive substances, were unexpectedly lower in SOD1 (-/-) mice at day 1 after wounding. The decay rate of electron paramagnetic resonance signal intensity of intravenously injected nitroxide radical indicated that the half-life of the signal intensity was significantly prolonged in the wounded skin of SOD1 (+/+) mice. However, while the half-life of the signal intensity in control skin was a little longer in SOD1 (-/-) mice, it did not change in wounded skin. Taken together, these data suggest that the skin of SOD1 (-/-) mice is in redox imbalance and prone to damage by wounding.
authors.affiliation: Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Yamagata University, 2-2-2 Iidanishi, Yamagata 990-9585, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Iuchi
authors.forename: Dipa
authors.initials: D
authors.lastname: Roy
authors.forename: Futoshi
authors.initials: F
authors.lastname: Okada
authors.forename: Noriko
authors.initials: N
authors.lastname: Kibe
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsunoda
authors.forename: Saori
authors.initials: S
authors.lastname: Suzuki
authors.forename: Motoko
authors.initials: M
authors.lastname: Takahashi
authors.forename: Hidekatsu
authors.initials: H
authors.lastname: Yokoyama
authors.forename: Jun
authors.initials: J
authors.lastname: Yoshitake
authors.forename: Seiji
authors.initials: S
authors.lastname: Kondo
authors.forename: Junichi
authors.initials: J
authors.lastname: Fujii
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D005143:Eyelids / Q000473:pathology; D005145:Face / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D018384:Oxidative Stress; D012871:Skin Diseases / Q000209:etiology*; D013482:Superoxide Dismutase / Q000172:deficiency*; D000072105:Superoxide Dismutase-1; D013997:Time Factors; D014945:Wound Healing
chemical_list: C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1