title: ELISA subtypization of anti-ENA autoantibodies in clinical management of autoimmune diseases in Bosnia and Herzegovina.
abstract: The basis of autoimmune diseases such as SLE (Systemic Lupus Eritematodes), Sjogren's syndrome, scleroderma, dermatomyositis and polymiositis is the creation of auto-antibodies to the following specific extractable nuclear antigens (ENA):Jo-1, Ssl-70, SS-A, SS-B, Sm and Sm/RNPs. Some of these antigens are in fact enzymes (Jo-1-histidil-tRNA synthetase, Scl-70-topoisomerase) which are inhibited by specific autoantibodies--this leads to disturbance in the metabolism of DNA and protein biosynthesis. During 2009, we analyzed total of 87 serum samples of patients suspected for autoimmune disorder using ANA-IFA and ELISA-ENA-6 methods. After establishing IFA-ANA positivity (83.9%), all serum specimens; ANA positive and negative, were subtypized by ELISA ENA-6 test. Analysis showed the highest incidence of anti-SS-A (56%), and incidence of anti-SS-B (29.8%), anti-Sm/ RNP (11.5%), anti-Jo-1 (2.3%) and anti-Scl-70 (1,1%) auto-antibodies. Also, 78.5% of IFA-ANA negative serum specimens showed high level of positivity (212.50 and 277.0 IU/ml) to SS-A (78.5%) and SS-B (21.4%) antigenes using ELISA-ENA-6 subtypization. Following these results, we conclude that it is necessary to introduce Western blot confirmation testing. After comparing with other clinical findings, we diagnosed the following autoimmune diseases: SLE, Sjogren's syndrome and dermatomiosytis.
authors.affiliation: Institute for Clinical Immunology, Clinical Centre University of Sarajevo, Bosnia and Herzegovina.
authors.forename: Djemo
authors.initials: D
authors.lastname: Subasic
authors.forename: Jasenko
authors.initials: J
authors.lastname: Karamehic
authors.forename: Faris
authors.initials: F
authors.lastname: Gavrankapetanovic
authors.forename: Harun
authors.initials: H
authors.lastname: Hodzic
authors.forename: Mersija
authors.initials: M
authors.lastname: Kasumovic
authors.forename: Marina
authors.initials: M
authors.lastname: Delic-Sarac
authors.forename: Lamija
authors.initials: L
authors.lastname: Prljaca-Zecevic
mesh_terms: D000974:Antibodies, Antinuclear / Q000097:blood*; D034961:Antigens, Nuclear / Q000276:immunology*; D001327:Autoimmune Diseases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans
chemical_list: D000974:Antibodies, Antinuclear; D034961:Antigens, Nuclear