title: [Application of zebrafish microarray on the toxicity mechanism study of bisphenol A].
abstract: The toxicity mechanism of bisphenol A (BPA) to zebrafish (Danio rerio) was studied in the molecular level,by the method of zebrafish microarray and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Zebrafish embryos were exposed to 0.5, 1.5 and 4.5 mg/L BPA for 8 days since fertilization. The results from microarray and validated by qRT-PCR showed that, 50 specific genes were up-or down-regulated, and dose-responses for them were significant (p < 0.05). This study demonstrates the genotoxicity of BPA. Moreover,according to their function and pathway analysis,it could support the mechanisms for the morphological toxicity and metabolize turbulence observed in prophase study.
authors.affiliation: Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China. duanzhenghua@mail.nankai.edu.cn
authors.forename: Zheng-hua
authors.initials: ZH
authors.lastname: Duan
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.forename: Ming-fen
authors.initials: MF
authors.lastname: Feng
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Bu
authors.forename: Siew Hong
authors.initials: SH
authors.lastname: Lam
authors.forename: Zhi-yuan
authors.initials: ZY
authors.lastname: Gong
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000633:toxicity*; D020411:Oligonucleotide Array Sequence Analysis; D010636:Phenols / Q000633:toxicity*; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds