Rapid and sensitive biomolecular screening with encoded macroporous hydrogel photonic beads.
We present a new method to prepare inverse opaline photonic beads with good spherical shape and superior optical performance by simply introducing an interfacial tension system into a template replication method. When the scaffolds of these beads were composed of poly(ethylene glycol) diacrylate hydrogel, they could provide a homogeneous water surrounding, which remedied many shortcomings of biomolecular microcarriers introduced by the presence of the solid surface of them. The suspension array, which used these macroporous hydrogel photonic beads as coding elements, showed obvious advantages in multiplexed capability, rapid biomolecular screening (within 12 min), and highly sensitive detection (with limit of detection of approximately 10(-12) M).
Zhao
Yuanjin
Y
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Zhao
Xiangwei
X
Tang
Baocheng
B
Xu
Wenyu
W
Gu
Zhongze
Z
D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D017785:Photons; D011092:Polyethylene Glycols / Q000737:chemistry; D016062:Porosity; D013997:Time Factors; D014867:Water / Q000737:chemistry
D020100:Hydrogels; D015345:Oligonucleotide Probes; C437167:poly(ethylene glycol)diacrylate; D014867:Water; D011092:Polyethylene Glycols; D004247:DNA