Use of cryopreserved cell aliquots in the high-throughput screening of small interfering RNA libraries.
Screening small interfering RNA (siRNA) libraries holds the potential to elucidate gene function as well as discover new targets for the therapeutic treatment of disease. Since the inception of siRNA as a discovery tool, there have been progressive improvements in siRNA design algorithms, the transfection reagents used to deliver them, and the assay formats used to monitor phenotypic changes. These changes have helped to improve the quality of the data emerging from siRNA screens. One variable that introduces inconsistency into high-throughput screening (HTS) of siRNA libraries is the state of the cells used in the assays. Multiple factors can contribute to differences in transfection efficiency as well as the basic cell biology, which can lead to differences in the genes identified in siRNA screens. The authors have developed a system using frozen cell aliquots to use in siRNA HTS, so that a major source of variability introduced into cell-based screens can be standardized. In addition, by transiently transfecting plasmids into cell lines and then freezing these cells down to use in siRNA transfection experiments, they have used this same technology to create new cell lines. This process of using aliquots of frozen cells is logistically advantageous in an HTS setting, as it reduces the time spent maintaining cell lines, as well as reducing possible downtime in screening due to lack of cells or poor cell health.
Swearingen
Elissa A
EA
Amgen, Inc., South San Francisco, CA 94080, USA.
Fajardo
Flordeliza
F
Wang
Xiangyun
X
Watson
J E Vivienne
JE
Quon
Kim C
KC
Kassner
Paul D
PD
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D015925:Cryopreservation; D015698:Genomic Library; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*
D034741:RNA, Small Interfering