[Effect of compound coarse grain food on insulin resistance and expression of PPARgamma in rat].
OBJECTIVE
To explore the effect of compound coarse grain food on insulin resistance (IR) in rats.


METHODS
Forty Sprague-Dawley rats were randomly divided into normal control group (n=10) and model group (n=30), provided regular diet and high fat diet separately. After 6 weeks, the model group were divided into high fat diet group, rice flour group and coarse grain group, and provided the corresponding feed. After feeding 9 weeks, the fasting blood glucose, insulin and the expression of PPAR-gamma mRNA in rats were measured.


RESULTS
IR was successfully induced by 6-weeks high fat diet. The body weight and insulin resistance index (HOMA-IR) in coarse grain group were significantly lower than high fat diet group and rice flour group. The expression of PPAR-gamma mRNA in coarse grain group was significantly higher than other three groups (P < 0.05).


CONCLUSIONS
The coarse grain food can reduce the concentration of blood glucose and insulin in rat with insulin resistance induced by high-fat dietary, and improve insulin sensitivity. The possible mechanism is the over-expression of PPAR-gamma.
Ding
Zhoubo
Z
School of Public Health, Southeast University, Nanjing 210009, China. dingzhoubo@yahoo.com.cn
Wang
Xiaofei
X
Zhai
Chengkai
C
Jin
Xin
X
Zhang
Qun
Q
Guo
Yanbo
Y
D000818:Animals; D004032:Diet; D002523:Edible Grain; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max; D014908:Triticum; D003313:Zea mays
D047495:PPAR gamma; D012333:RNA, Messenger