Cooperativity and frustration in protein-mediated parallel actin bundles.
We examine the mechanism of bundling of cytoskeletal actin filaments by two representative bundling proteins, fascin and espin. Small-angle x-ray studies show that increased binding from linkers drives a systematic overtwist of actin filaments from their native state, which occurs in a linker-dependent fashion. Fascin bundles actin into a continuous spectrum of intermediate twist states, while espin only allows for untwisted actin filaments and fully overtwisted bundles. Based on a coarse-grained, statistical model of protein binding, we show that the interplay between binding geometry and the intrinsic flexibility of linkers mediates cooperative binding in the bundle. We attribute the respective continuous (discontinuous) bundling mechanisms of fascin (espin) to difference in the stiffness of linker bonds themselves.
Shin
Homin
H
Department of Polymer Science and Engineering, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Purdy Drew
Kirstin R
KR
Bartles
James R
JR
Wong
Gerard C L
GC
Grason
Gregory M
GM
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D006801:Humans; D008840:Microfilament Proteins / Q000378:metabolism*; D051381:Rats; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
D000199:Actins; D033942:Adaptor Proteins, Vesicular Transport; D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D008840:Microfilament Proteins; C114154:epsin; C020324:fascin