Increased prolyl endopeptidase activity in human neoplasia.
Prolyl endopeptidase (EC 3.4.21.26) (PEP) is a serine peptidase that converts several biologically active peptides. This enzyme has been linked to several neurological, digestive, cardiovascular and infectous disorders. However, little is known about its involvement in neoplastic processes. This study analyzes fluorimetrically cytosolic and membrane-bound PEP activity in a large series (n=122) of normal and neoplastic tissues from the kidney, colon, oral cavity, larynx, thyroid gland and testis. Cytosolic PEP activity significantly increased in clear cell renal cell carcinoma, urothelial carcinoma of the renal pelvis and head and neck squamous cell carcinoma. Both cytosolic and membrane-bound PEP activity were also increased in colorectal adenomatous polyps. These data suggest the involvement of PEP in some mechanisms that underlie neoplastic processes.
Larrinaga
Gorka
G
Department of Physiology, School of Medicine and Dentistry, Leioa, Bizkaia, Spain. gorka.larrinaga@ehu.es
Perez
Itxaro
I
Blanco
Lorena
L
López
José I
JI
Andrés
Leire
L
Etxezarraga
Carmen
C
Santaolalla
Francisco
F
Zabala
Aitor
A
Varona
Adolfo
A
Irazusta
Jon
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000378:metabolism; D000085822:Prolyl Oligopeptidases; D012697:Serine Endopeptidases / Q000378:metabolism*; D013736:Testicular Neoplasms / Q000201:enzymology* / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000201:enzymology* / Q000378:metabolism
D012697:Serine Endopeptidases; C000705287:PREPL protein, human; D000085822:Prolyl Oligopeptidases