Evaluation of cytotoxicity of two root canal filling materials by MTT assay.
The aim of this study was to assess the cytotoxicity of two root filling materials GuttaFlow (GF) and gutta-percha (GP) on mouse fibroblasts cell line L-929. In this study there were four groups: GP and GF were considered as study groups and the other two were negative control groups. GP and GF were prepared according to manufacturer's instruction. L-929 fibroblast cells of mouse were passaged with trypsin (Merck, Germany) after elimination of freeze phase. Adequate trypsin was added to cells and they were prepared with 95% of cell vitality. After 24 h, 150,000 cells were put in each well. The cell and dimethyl methacrylate were used as negative and positive controls. Ten specimens from each group were brought into contact with the culture medium and were incubated under sterilised conditions 24 h later. The cytotoxicity of all samples was assessed by dimethylthiazol diphenyltetrazolium bromide test after 1 h, 24 h and 72 h. The results showed that cytotoxicity of GF was less than GP when assessed at 24 h and 72 h, but there was no significant difference at 1 h. In GF, the most and least cytotoxicity were observed at 24 h and 72 h while cytotoxicity of GP increased with time.
Ashraf
Hengameh
H
Department of Endodontics, School of Dentistry, Shaheed Beheshti Medical University, Evin, Tehran, Iran. he_ashraaf@yahoo.com
Taherian
Ali
A
Kerdar
Alireza N
AN
D000818:Animals; D004129:Dimethylpolysiloxanes / Q000633:toxicity*; D004338:Drug Combinations; D005347:Fibroblasts / Q000187:drug effects*; D006171:Gutta-Percha / Q000633:toxicity*; D007739:L Cells; D008422:Materials Testing; D051379:Mice; D012387:Root Canal Filling Materials / Q000633:toxicity*; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D013997:Time Factors
D004129:Dimethylpolysiloxanes; D004338:Drug Combinations; C515718:GuttaFlow; D012387:Root Canal Filling Materials; D013778:Tetrazolium Salts; D013844:Thiazoles; D006171:Gutta-Percha; C022616:thiazolyl blue