title: UCP3 translocates lipid hydroperoxide and mediates lipid hydroperoxide-dependent mitochondrial uncoupling.
abstract: Although the literature contains many studies on the function of UCP3, its role is still being debated. It has been hypothesized that UCP3 may mediate lipid hydroperoxide (LOOH) translocation across the mitochondrial inner membrane (MIM), thus protecting the mitochondrial matrix from this very aggressive molecule. However, no experiments on mitochondria have provided evidence in support of this hypothesis. Here, using mitochondria isolated from UCP3-null mice and their wild-type littermates, we demonstrate the following. (i) In the absence of free fatty acids, proton conductance did not differ between wild-type and UCP3-null mitochondria. Addition of arachidonic acid (AA) to such mitochondria induced an increase in proton conductance, with wild-type mitochondria showing greater enhancement. In wild-type mitochondria, the uncoupling effect of AA was significantly reduced both when the release of O2* in the matrix was inhibited and when the formation of LOOH was inhibited. In UCP3-null mitochondria, however, the uncoupling effect of AA was independent of the above mechanisms. (ii) In the presence of AA, wild-type mitochondria released significantly more LOOH compared with UCP3-null mitochondria. This difference was abolished both when UCP3 was inhibited by GDP and under a condition in which there was reduced LOOH formation on the matrix side of the MIM. These data demonstrate that UCP3 is involved both in mediating the translocation of LOOH across the MIM and in LOOH-dependent mitochondrial uncoupling.
authors.affiliation: Dipartimento delle Scienze Biologiche, Università degli Studi di Napoli Federico II, Via Mezzocannone 8, 80134 Napoli, Italy. assunta.lombardi@unina.it
authors.forename: Assunta
authors.initials: A
authors.lastname: Lombardi
authors.forename: Rosa Anna
authors.initials: RA
authors.lastname: Busiello
authors.forename: Laura
authors.initials: L
authors.lastname: Napolitano
authors.forename: Federica
authors.initials: F
authors.lastname: Cioffi
authors.forename: Maria
authors.initials: M
authors.lastname: Moreno
authors.forename: Pieter
authors.initials: P
authors.lastname: de Lange
authors.forename: Elena
authors.initials: E
authors.lastname: Silvestri
authors.forename: Antonia
authors.initials: A
authors.lastname: Lanni
authors.forename: Fernando
authors.initials: F
authors.lastname: Goglia
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000737:chemistry; D007473:Ion Channels / Q000378:metabolism* / Q000502:physiology*; D007700:Kinetics; D008054:Lipid Peroxides / Q000737:chemistry*; D051379:Mice; D008822:Mice, Transgenic; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism* / Q000502:physiology*; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011522:Protons; D000071256:Uncoupling Protein 1; D000071247:Uncoupling Protein 3
chemical_list: D007473:Ion Channels; D008054:Lipid Peroxides; D024101:Mitochondrial Proteins; D011522:Protons; C000604393:Ucp3 protein, mouse; D000071256:Uncoupling Protein 1; D000071247:Uncoupling Protein 3; D016718:Arachidonic Acid; D010100:Oxygen