title: The inflammasome mediates hyperoxia-induced alveolar cell permeability.
abstract: A hallmark of hyperoxic acute lung injury is the influx of inflammatory cells to lung tissue and the production of proinflammatory cytokines, such as IL-1beta; however, the mechanisms connecting hyperoxia and the inflammatory response to lung damage is not clear. The inflammasome protein complex activates caspase-1 to promote the processing and secretion of proinflammatory cytokines. We hypothesized that hyperoxia-induced K(+) efflux activates the inflammasome via the purinergic P2X7 receptor to cause inflammation and hyperoxic acute lung injury. To test this hypothesis, we characterized the expression and activation of inflammasome components in primary murine alveolar macrophages exposed to hyperoxia (95% oxygen and 5% CO(2)) in vitro, and in alveolar macrophages isolated from mice exposed to hyperoxia (100% oxygen). Our results showed that hyperoxia increased K(+) efflux, inflammasome formation, release of proinflammatory cytokines, and induction of caspase-1 and IL-1beta cleavage both in vitro and in vivo. The P2X7 agonist ATP enhanced hyperoxia-induced inflammasome activation, whereas the P2X7 antagonist, oxidized ATP, inhibited hyperoxia induced inflammasome activation. In addition, when ATP was scavenged with apyrase, hyperoxia-induced inflammasome activation was significantly decreased. Furthermore, short hairpin RNA silencing of inflammasome components abrogated hyperoxia-induced secretion of proinflammatory cytokines in vitro. These results suggest that hyperoxia induces K(+) efflux through the P2X7 receptor, leading to inflammasome activation and secretion of proinflammatory cytokines. These events would affect the permeability of the alveolar epithelium and ultimately lead to epithelial barrier dysfunction and cell death.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Narasaiah
authors.initials: N
authors.lastname: Kolliputi
authors.forename: Rahamthulla S
authors.initials: RS
authors.lastname: Shaik
authors.forename: Aaron B
authors.initials: AB
authors.lastname: Waxman
mesh_terms: D055371:Acute Lung Injury / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D000255:Adenosine Triphosphate / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D002463:Cell Membrane Permeability / Q000276:immunology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D018496:Hyperoxia / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D018836:Inflammation Mediators / Q000502:physiology*; D053583:Interleukin-1beta / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011188:Potassium / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000276:immunology; D021381:Protein Transport / Q000276:immunology; D011650:Pulmonary Alveoli / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D020545:Respiratory Mucosa / Q000276:immunology / Q000378:metabolism / Q000473:pathology
chemical_list: D018836:Inflammation Mediators; D053583:Interleukin-1beta; D000255:Adenosine Triphosphate; D011188:Potassium