Methods for the isolation of sensory and primary cilia--an overview.
Detailed proteomic analyses of mammalian olfactory and rod photoreceptor sensory cilia are now available, providing an inventory of resident ciliary proteins and laying the foundation for future studies of developmental and spatiotemporal changes in the composition of sensory cilia. Cilia purification methods that were elaborated and perfected over several decades were essential for these advances. In contrast, the proteome of primary cilia is yet to be established, because purification procedures for this organelle have been developed only recently. In this chapter, we review current techniques for the purification of olfactory and photoreceptor cilia, and evaluate methods designed for the selective isolation of primary cilia.
Mitchell
Kimberly A P
KA
Department of Biology and Chemistry, Liberty University, Lynchburg, Virginia 24502, USA.
Szabo
Gabor
G
Otero
Angela de S
Ade S
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D002923:Cilia / Q000737:chemistry* / Q000648:ultrastructure; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D018034:Olfactory Receptor Neurons / Q000648:ultrastructure; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D017948:Retinal Rod Photoreceptor Cells / Q000648:ultrastructure; D033081:Shear Strength; D013314:Stress, Mechanical; D013347:Subcellular Fractions / Q000737:chemistry
D011506:Proteins; D002118:Calcium