[Differentiation of geographic biovariants of smallpox virus by PCR].
Comparative analysis of amino acid and nucleotides sequences of ORFs located in extended segments of the terminal variable regions in variola virus genome detected a promising locus for viral genotyping according to the geographic origin. This is ORF O1L of VARV. The primers were calculated for synthesis of this ORF fragment by PCR, which makes it possible to distinguish South America-Western Africa genotype from other VARV strains. Subsequent RFLP analysis reliably differentiated Asian strains from African strains (except Western Africa isolates). This method has been tested using 16 VARV strains from various geographic regions. The developed approach is simple, fast and reliable.
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D000349:Africa; D000595:Amino Acid Sequence; D017089:Ankyrin Repeat / Q000235:genetics; D001208:Asia; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D016679:Genome, Viral / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D012899:Smallpox / Q000821:virology*; D013020:South America; D012901:Variola virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers