Phospholipidosis as a function of basicity, lipophilicity, and volume of distribution of compounds.
Drug-induced phospholipidosis (PLD) is an adaptive histologic alteration that is seen with various marketed drugs and often encountered during drug development. Various in silico and in vitro cell-based methods have been developed to predict the PLD-inducing potential of compounds. These methods rely on the inherent physicochemical properties of the molecule and, as such, tend to overpredict compounds as PLD inducers. Recognizing that the distribution of compounds into tissues or tissue accumulation is likely a key factor in PLD induction, in addition to key physicochemical properties, we developed a model to predict PLD in vivo using the measures of basicity (pK(a)), lipophilicity (ClogP), and volume of distribution (V(d)). Using sets of PLD inducers and noninducers, we demonstrate improved concordance with this method. Furthermore, we propose a screening paradigm that includes a combination of various methods to predict the in vivo PLD-inducing potential of compounds, which may be especially useful in lead identification and optimization processes in drug discovery.
Hanumegowda
Umesh M
UM
Department of Discovery Toxicology, Bristol-Myers Squibb Research and Development, 5 Research Parkway, Wallingford, Connecticut 06492, USA. umesh.hanumegowda@bms.com
Wenke
Gottfried
G
Regueiro-Ren
Alicia
A
Yordanova
Roumyana
R
Corradi
John P
JP
Adams
Stephen P
SP
D000465:Algorithms; D000818:Animals; D064420:Drug-Related Side Effects and Adverse Reactions; D006801:Humans; D008064:Lipidoses / Q000139:chemically induced* / Q000503:physiopathology; D008958:Models, Molecular; D015233:Models, Statistical; D010743:Phospholipids / Q000378:metabolism; D051381:Rats
D010743:Phospholipids