Enrichment of low-abundance proteins from bovine and porcine serum samples for proteomic studies.
One of the main applications of serum proteomics is the identification of new biomarkers for animal disease or animal production. However, potential obstacles to these studies are the poor performance of affinity serum depletion methods based on human antigens when using animal samples, and loss of minor serum components bound to albumin and other proteins. In the present study, we have analyzed the efficiency and reproducibility of the ProteoMiner® beads with bovine and porcine serum samples, and compared to a traditional immunoaffinity-based albumin and IgG depletion system specific for human samples. The ProteoMiner kit is based on the use of a combinatorial peptide binding library and intends to enrich low-abundance proteins.
Marco-Ramell
Anna
A
Departament de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain.
Bassols
Anna
A
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification*; D002417:Cattle / Q000097:blood* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D007074:Immunoglobulin G / Q000235:genetics / Q000302:isolation & purification; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000235:genetics / Q000302:isolation & purification; D013552:Swine / Q000097:blood* / Q000235:genetics
D001798:Blood Proteins; D007074:Immunoglobulin G; D012709:Serum Albumin