title: Novel Escherichia coli hybrids with enhanced butanol tolerance.
abstract: Hybrids between Escherichia coli and Lactobacillus brevis were generated via protoplast fusion. Growth kinetics of five hybrid strains and E. coli were used to evaluate the butanol tolerance of the novel strains under different conditions. The hybrid strains tolerated up to 2% (v/v) butanol compared to the 1% (v/v) maximum for E. coli. The growth inhibitory effects of butanol were also significantly less in several of the hybrids compared to E. coli. These results demonstrate the potential use of protoplast fusion to generate butanol-tolerant strains.
authors.affiliation: Department of Chemical Engineering, Texas A&M University, College Station, TX, 77843, USA.
authors.forename: James
authors.initials: J
authors.lastname: Winkler
authors.forename: Matthew
authors.initials: M
authors.lastname: Rehmann
authors.forename: Katy C
authors.initials: KC
authors.lastname: Kao
mesh_terms: D000440:Butanols / Q000633:toxicity*; D003433:Crosses, Genetic; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development*; D052196:Levilactobacillus brevis / Q000235:genetics*
chemical_list: D000440:Butanols