The synergistic antibacterial activity of 1-acetyl-beta-carboline and beta-lactams against methicillin-resistant Staphylococcus aureus (MRSA).
1-Acetyl-beta-carboline was isolated as an anti-MRSA agent from the fermentation broth of a marine actinomycete isolated from marine sediment. The producing strain was identified to be Streptomyces sp. by phylogenetic analysis of the 16S rRNA gene sequence. The anti-MRSA agent was isolated by bioactivity-guided fractionation of the culture extract by solvent partitioning, ODS open flash chromatography, and purification with a reversed-phase HPLC. Its structure was elucidated by extensive 2D NMR and mass spectral analyses. Combination of 1-acetyl-beta- carboline with ampicillin exhibited synergistic antibacterial activity against MRSA.
Shin
Hee Jae
HJ
Marine Natural Products Laboratory, Korea Ocean Research and Development Institute, Ansan, Seoul 425-600, Korea.
Lee
Hyi-Seung
HS
Lee
Dae-Sung
DS
D000667:Ampicillin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002243:Carbolines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004357:Drug Synergism; D019015:Geologic Sediments; D013058:Mass Spectrometry; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D019906:Nuclear Magnetic Resonance, Biomolecular; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D002243:Carbolines; D000667:Ampicillin