Growth factor production from fibrin-encapsulated human keratinocytes.
Fibrin has been used extensively in cell encapsulation because it has important biological properties. Keratinocyte encapsulation in fibrin is a widely used technique in skin tissue engineering. The production of growth factors (EGF, TGF-beta1 and PDGF-BB) was evaluated when keratinocytes are encapsulated in fibrin. Secretions of TGF-beta1 and PDGF-BB increased more than five times compared to monolayer cultures. Encapsulated cells secreted about 80% active form of TGF-beta1 (monolayer cells only secreted inactive form). An enhanced secretion of TGF-beta1 and PDGF-BB was found in encapsulated cells, showing that fibrin capsules are favourable for the production of these growth factors.
Acevedo
Cristian A
CA
Centro de Biotecnología, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile. cristian.acevedo@usm.cl
Somoza
Rodrigo A
RA
Weinstein-Oppenheimer
Caroline
C
Brown
Donald I
DI
Young
Manuel E
ME
D018929:Cell Culture Techniques; D002478:Cells, Cultured; D005337:Fibrin / Q000378:metabolism*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism*
D036341:Intercellular Signaling Peptides and Proteins; D005337:Fibrin