title: Differential expression of KCNQ1 K+ channel in tubular cells of frog kidney.
abstract: The aim of this study was to evaluate KCNQ1 K+ channel expression in the frog kidney of Rana esculenta. KCNQ1 K+ channel, also known as KvLQT1, is the pore forming a-subunit of the IKs K+ channel, a delayed rectifier voltage-gated K+ channel, which has an important role in water and salt transport in the kidney and gastrointestinal tract. The expression of KCNQ1 K+ channel along tubular epithelium differs from species to species. In the present study the expression of KCNQ1 K+ channel in the frog kidney has been demonstrated by immunohistochemistry. The presence of KCNQ1 K+ channel was demonstrated in the epithelial cells of distal convoluted tubule and collecting duct. However, the pattern of expression of KCNQ1 K+ channel differs between distal convoluted tubules and collecting duct. All epithelial cells of distal convoluted tubules revealed basolateral expression of KCNQ1 K+ channel. On the contrary, only the single cells of collecting duct, probably intercalated cells, showed diffuse cell surface staining with antibodies against KCNQ1 K+ channel. These findings suggest that KCNQ1 K+ channel has cell-specific roles in renal potassium ion transport.
authors.affiliation: University of Belgrade, Serbia.
authors.forename: S
authors.initials: S
authors.lastname: Cirović
authors.forename: J
authors.initials: J
authors.lastname: Marković-Lipkovski
authors.forename: J
authors.initials: J
authors.lastname: Todorović
authors.forename: J
authors.initials: J
authors.lastname: Nesović-Ostojić
authors.forename: M
authors.initials: M
authors.lastname: Jović
authors.forename: S
authors.initials: S
authors.lastname: Ilić
authors.forename: S
authors.initials: S
authors.lastname: Tatić
authors.forename: D
authors.initials: D
authors.lastname: Cemerikić
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D051657:KCNQ1 Potassium Channel / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D008297:Male; D011893:Rana esculenta
chemical_list: D051657:KCNQ1 Potassium Channel; C496909:KCNQ1 protein, human