Peptidomic analysis of the skin secretions of the frog Pachymedusa dacnicolor.
High-resolution mass spectrometry-based peptidomics has been used to characterize several components in electro-stimulated skin secretions of the endemic Mexican frog Pachymedusa dacnicolor. Peptide mass screening performed in an Orbitrap-XL mass spectrometer showed that P. dacnicolor skin secretions possess 194 different components with molecular masses ranging mainly from 500 to 6,000 Da. Dozens of molecules were partially sequenced including two novel protease inhibitors. Additionally, one posttranslationally modified bradykinin and two novel dermaseptin-like antimicrobial peptides were fully sequenced. The novel peptide named here DMS-DA5 was fully characterized and showed potent antibacterial activity against various bacteria such as Escherichia coli, Bacillus subtilis, Salmonella enterica serovar typhimurium, and Pseudomonas aeruginosa with minimal inhibitory concentrations from 3.10 to 25.0 microM.
Meneses
Erika P
EP
Unidad de Proteómica-Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Av. Universidad, 2001 Col. Chamilpa, CP 62210, Cuernavaca, Morelos, Mexico.
Villa-Hernández
Oscar
O
Hernández-Orihuela
Lorena
L
Castro-Franco
Ruben
R
Pando
Victoria
V
Aguilar
Manuel B
MB
Batista
Cesar Vicente Ferreira
CV
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism; D001001:Anura / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D016415:Sequence Alignment; D012867:Skin / Q000737:chemistry* / Q000378:metabolism
D000900:Anti-Bacterial Agents