[The establishment and evaluation of diagnostic accuracy of AllGlo(TM) probe-based techniques for invasive aspergillosis.].
OBJECTIVE
To establish and evaluate a diagnostic technique based on the AllGlo(TM) probe for the invasive aspergillosis.


METHODS
With the self-designed AllGlo(TM) probes and primers and the standards, two standard curves of the real-time PCR based on AllGlo(TM) probes were established respectively for A. flavus and A. fumigatus, then its specificity, sensitivity and reproducibility were evaluated respectively.


RESULTS
The findings indicated that the standard curve of A. flavus was Y = -3.003X + 36.825, and A.fumigatus' was Y = -3.052X + 38.016, and their interassay coefficient of variation respectively were 15.60% and 12.94%, suggesting a good reproducibility. The lowest spore concentration they could be detected was 10 CFU/ml, which equated to 100 - 1000 copies of internal transcribed spacer (ITS)2 genes, suggesting a good sensibility. They didn't have cross-positive reaction with other fungus, human genome and bacteria, which indicated a good specificity.


CONCLUSIONS
The diagnostic technique based on the AllGlo(TM) probe for the invasive aspergillosis possessed a good sensitivity, good specificity and deadly accuracy.
Wu
Dan-Sen
DS
Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, China. Email: Brace@medmail.com.cn.
Shen
Jian-Zhen
JZ
Zhou
Xiao-Qiang
XQ
Shen
Song-Fei
SF
Wu
Xue-Mei
XM
D001228:Aspergillosis; D001232:Aspergillus fumigatus; D001992:Bronchoalveolar Lavage Fluid; D017931:DNA Primers; D006801:Humans; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D017931:DNA Primers