Rapid serodiagnosis with the use of surface plasmon resonance imaging for the detection of antibodies against major surface protein A of Mycoplasma synoviae in chickens.
Mycoplasma synoviae, a major worldwide pathogen in poultry, causes respiratory tract infection and arthritis in chickens and turkeys. Two major surface antigens of M. synoviae are encoded by a single gene, vlhA (variably expressed lipoprotein and hemagglutinin). The gene product is cleaved post-translationally to yield the lipoprotein major surface protein (MSP) B (MSPB) and the hemagglutinin MSPA. The availability of MSPA as an antigen for serodiagnosis was studied by means of a protein chip based on surface plasmon resonance imaging (SPRi). The diagnostic potential of SPRi for measurement of levels of antibody to MSPA was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) kit. The results from SPRi, a process that took only 1 h, were similar to those from ELISA. Therefore, MSPA can be used as an antigen for serologic studies, and SPRi, a label-free and high-throughput method, may be a valuable tool in avian serodiagnostic studies.
Oh
Kiseok
K
College of Veterinary Medicine, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, Republic of Korea.
Lee
Semi
S
Seo
Jayoung
J
Lee
Dongwoo
D
Kim
Taejung
T
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001715:Bird Diseases / Q000097:blood / Q000175:diagnosis*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D006388:Hemagglutinins / Q000276:immunology; D009175:Mycoplasma Infections / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D045805:Mycoplasma synoviae / Q000276:immunology / Q000302:isolation & purification*; D040081:Protein Array Analysis / Q000379:methods; D012698:Serologic Tests / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D006388:Hemagglutinins