[Combination of real-time polymerase chain reaction assay and serum galactomannan in the diagnosis of invasive aspergillosis in patients with hematological malignancies and recipients of hematopoietic stem cell transplantation].
OBJECTIVE
To evaluate the role of real-time polymerase chain reaction (real-time PCR) assay combined with serum galactomannan (GM) detection in the diagnosis of invasive aspergillosis (IA) with hematological malignancies or hematopoietic stem cell transplantation.


METHODS
Eighty patients were enrolled. PCR and GM were performed simultaneously on 205 serum samples. The agreement between these two assays and the diagnostic value of the combination were analyzed.


RESULTS
There were 5 proven IA, 20 probable IA, 34 possible IA and 21 non-IA. Agreement between real-time PCR and GM was 77.5%. When both positive, the positive predictive value could reach 100%; when both negative, the negative predictive value could reach 90.9%.


CONCLUSIONS
PCR agrees well with GM. When these two assays are combined, invasive aspergillosis can be diagnosed more accurately.
Sun
Yu-qian
YQ
Institute of Hematology & People's Hospital, Peking University, Beijing 100044, China.
Ji
Yu
Y
Xu
Lan-ping
LP
Liu
Dai-hong
DH
Liu
Kai-yan
KY
Huang
Xiao-jun
XJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D001228:Aspergillosis / Q000175:diagnosis*; D002648:Child; D005260:Female; D005690:Galactose / Q000031:analogs & derivatives; D019337:Hematologic Neoplasms; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D008351:Mannans / Q000097:blood*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D044967:Serum / Q000737:chemistry; D055815:Young Adult
D008351:Mannans; C012990:galactomannan; D005690:Galactose