title: Genetic identification of new biological species Saccharomyces arboricolus Wang et Bai.
abstract: Direct genetic testing for hybrid sterility unambiguously showed that the newly described yeast Saccharomyces arboricolus Wang et Bai is reproductively isolated from Saccharomyces cerevisiae, Saccharomyces bayanus, Saccharomyces cariocanus, Saccharomyces kudriavzevii, Saccharomyces mikatae and Saccharomyces paradoxus and, therefore, represents a new biological species of the genus Saccharomyces. Combined phylogenetic analysis of the rDNA repeat sequences (18S, 26S, ITS), nuclear ACT1 and mitochondrial ATP9 genes revealed that S. arboricolus, along with S. kudriavzevii and S. bayanus, is distantly related to the other four biological species.
authors.affiliation: State Institute for Genetics and Selection of Industrial Microorganisms, Moscow 117545, Russia. gnaumov@yahoo.com
authors.forename: Gennadi I
authors.initials: GI
authors.lastname: Naumov
authors.forename: Elena S
authors.initials: ES
authors.lastname: Naumova
authors.forename: Isabelle
authors.initials: I
authors.lastname: Masneuf-Pomarède
mesh_terms: D000199:Actins / Q000235:genetics; D016000:Cluster Analysis; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D000199:Actins; D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; C065096:RNA, ribosomal, 26S; D025261:Mitochondrial Proton-Translocating ATPases