Model of gene transcription including the return of a RNA polymerase to the beginning of a transcriptional cycle.
The gene transcription occurs via the RNA polymerase (RNAP) recruitment on the DNA promoter sequence, formation of a locally open DNA chain, promoter escape, steps of the RNA synthesis, and RNA and RNAP release after reading the final DNA base. Just after the end of the RNA synthesis, RNAP surrounds the closed DNA chain and may diffuse along DNA, desorb, or reach the promoter and start the RNA-synthesis cycle again. We present a generic kinetic model taking the latter steps into account and show analytically and by Monte Carlo simulations that it predicts transcriptional bursts even in the absence of explicit regulation of the transcription by master proteins.
Zhdanov
Vladimir P
VP
Division of Biological Physics, Department of Applied Physics, Chalmers University of Technology, S-41296 Göteborg, Sweden. zhdanov@catalysis.ru
D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000235:genetics*; D008956:Models, Chemical; D008957:Models, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015533:Transcriptional Activation / Q000502:physiology*
D004247:DNA; D012321:DNA-Directed RNA Polymerases