Fluorescent nano-optodes for glucose detection.
We have designed fluorescent nanosensors based on ion-selective optodes capable of detecting small molecules. By localizing the sensor components in a hydrophobic core, these nanosensors are able to monitor dynamic changes in concentration of the model analyte, glucose. The nanosensors demonstrated this response in vitro and also when injected subcutaneously into mice. The response of the nanosensors tracked changes in blood glucose levels in vivo that were comparable to measurements taken using a glucometer. The development of these nanosensors offers an alternative, minimally invasive tool for monitoring glucose levels in such fields as diabetes research. Furthermore, the extension of the ion-selective optode sensor platform to small molecule detection will allow for enhanced monitoring of physiological processes.
Billingsley
Kelvin
K
Biomedical Engineering Group, The Charles Stark Draper Laboratory, 555 Technology Square, Cambridge, Massachusetts 02139, USA.
Balaconis
Mary K
MK
Dubach
J Matthew
JM
Zhang
Ning
N
Lim
Ed
E
Francis
Kevin P
KP
Clark
Heather A
HA
D000818:Animals; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis* / Q000737:chemistry; D001897:Boronic Acids / Q000737:chemistry; D005456:Fluorescent Dyes; D005947:Glucose / Q000032:analysis*; D051379:Mice; D015394:Molecular Structure; D036103:Nanotechnology
D001786:Blood Glucose; D001897:Boronic Acids; D005456:Fluorescent Dyes; D005947:Glucose