Cardiovascular phenotyping of the mouse heart using a 4D radial acquisition and liposomal Gd-DTPA-BMA.
MR microscopy has enormous potential for small-animal cardiac imaging because it is capable of producing volumetric images at multiple time points to accurately measure cardiac function. MR has not been used as frequently as ultrasound to measure cardiac function in the small animal because the MR methods required relatively long scan times, limiting throughput. Here, we demonstrate four-dimensional radial acquisition in conjunction with a liposomal blood pool agent to explore functional differences in three populations of mice: six C57BL/6J mice, six DBA/2J mice, and six DBA/2J CSQ+ mice, all with the same gestational age and approximately the same weight. Cardiovascular function was determined by measuring both left ventricular and right ventricular end diastolic volume, end systolic volume, stroke volume, and ejection fraction. Statistical significance was observed in end diastolic volume, end systolic volume, and ejection fraction for left ventricular measurements between all three populations of mice. No statistically significant difference was observed in stroke volume in either the left or right ventricle for any of the three populations of mice. This study shows that MRI is capable of efficient, high-throughput, four-dimensional cardiovascular phenotyping of the mouse.
Bucholz
Elizabeth
E
Center for In Vivo Microscopy, Duke University, Durham, North Carolina, USA.
Ghaghada
Ketan
K
Qi
Yi
Y
Mukundan
Srinivasan
S
Rockman
Howard A
HA
Johnson
G Allan
GA
D000465:Algorithms; D000704:Analysis of Variance; D000818:Animals; D002320:Cardiovascular Physiological Phenomena; D003287:Contrast Media / Q000737:chemistry; D019786:Gadolinium DTPA / Q000737:chemistry; D021621:Imaging, Three-Dimensional; D008081:Liposomes; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010641:Phenotype
D003287:Contrast Media; D008081:Liposomes; C064925:gadodiamide; D019786:Gadolinium DTPA