Improving the anti-tumor effect of genistein with a biocompatible superparamagnetic drug delivery system.
The practical application of genistein as a low toxicity chemotherapeutic drug is hindered by many of its in vivo properties. To overcome these obstacles, a new multifunctional drug delivery system is developed, which is based on covalently attaching genistein onto Fe3O4 nanoparticles coated by cross-linked carboxymethylated chitosan (CMCH). The structure of the Fe3O4-CMCH-genistein nano-conjugate was confirmed by transmission electron micrographs (TEM), X-ray diffraction (XRD) and Fourier-transfer infrared (FT-IR) spectroscopy. The nano-conjugate shows good water solubility and superparamagnetic properties with a saturation magnetization of 55.1 emu/g. The effects of free genistein and FeO4-CMCH-genistein nano-conjugate on the proliferation and apoptosis of gastric cancer cell line SGC-7901 were investigated by MTT assay and flow cytometry (FACS). MTT results indicate that the Fe3O4-CMCH-genistein nano-conjugate exhibits a significantly enhanced inhibition effect to the SGC-7901 cancer cells than the free genistein. FACS data suggests that the inhibition on cell proliferation of the nano-conjugate is related with an induced apoptosis process. This drug delivery system is promising for future multifunctional chemotherapeutic application that combines drug release and magnetic hyperthermia therapy.
Si
Hua-Yan
HY
State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.
Li
Dong-Peng
DP
Wang
Tian-Ming
TM
Zhang
Hao-Li
HL
Ren
Fang-Yuan
FY
Xu
Zhu-Guo
ZG
Zhao
Yu-Yuan
YY
D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000528:radiation effects; D004574:Electromagnetic Fields; D005290:Ferric Compounds / Q000737:chemistry* / Q000528:radiation effects; D019833:Genistein / Q000008:administration & dosage* / Q000737:chemistry*; D006801:Humans; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*
D016588:Anticarcinogenic Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D005290:Ferric Compounds; C000499:ferric oxide; D019833:Genistein