Cloning, expression and physiological analysis of broccoli catalase gene and Chinese cabbage ascorbate peroxidase gene under heat stress.
The objectives of this work were to clone the catalase (CAT) gene from broccoli (Brassica oleracea) and the ascorbate peroxidase (APX) gene from Chinese cabbage and measure the regulation of CAT and APX gene expressions under heat-stress conditions. Different genotypes responded differently to heat stress according to their various antioxidant enzymes and physiological parameters. CAT and APX gene expression profiles were well matched with the data for CAT and APX enzyme activities in the broccoli and Chinese cabbage plants, respectively. Full-length of the CAT and APX cDNA were 1,768 and 1,070 bp, respectively. A phylogenetic analysis of CAT and APX indicated that plant CATs and APXs diverged into two major clusters.
Lin
Kuan-Hung
KH
Graduate Institute of Biotechnology, Chinese Culture University, 55 Hwa-Kong Road, Taipei, 111, Taiwan, ROC. rlin@faculty.pccu.edu.tw
Huang
Ho-Chang
HC
Lin
Ching-Yun
CY
D000595:Amino Acid Sequence; D060387:Ascorbate Peroxidases; D001483:Base Sequence; D001937:Brassica / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D006358:Hot Temperature; D015227:Lipid Peroxidation; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological
D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase