Life cycle analysis of unicellular algae.
Unicellular green alga is a very convenient object for flow cytometric characterization. Flow cytometry has been proposed as a quick and reliable tool for studying life cycle and growth of unicellular algae. Cell size of vegetating algae can be monitored in association with their DNA and endogenous chlorophyll content. Cells of interest (e.g., group of cells of a certain stage of the life cycle) in an asynchronously proliferating cell population can be sorted out for further microscopical or molecular biology studies. This methodological approach can be helpful for researchers who are interested in algal proliferation.
Gerashchenko
Bogdan I
BI
RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, Ukraine.
Takahashi
Toshiyuki
T
Kosaka
Toshikazu
T
Hosoya
Hiroshi
H
D018929:Cell Culture Techniques; D002734:Chlorophyll / Q000737:chemistry; D000460:Chlorophyta / Q000378:metabolism / Q000502:physiology*; D003584:Cytological Techniques; D004247:DNA / Q000378:metabolism; D048028:DNA, Algal / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*
D048028:DNA, Algal; D002734:Chlorophyll; D004247:DNA