[Protective effects of resveratrol on neonatal rat cardiomyocyte lesion induced by hypoxia].
OBJECTIVE
To investigate the effects of resveratrol (Res) on neonatal rat cardiomyocyte lesion induced by hypoxia.


METHODS
The cardiomyocyte of neonatal rats were cultured in vitro and the model of cardiomyocyte hypoxia was established. The cardiomyocyte vitalities were determined by MTT assay, the HIF-1alpha expression levels in myocardial cells was detected by immunohistochemical, the activities of peroxidase (GSH-Px) and lactate dehydrogenase (LDH) were measured as well.


RESULTS
After the administration of hypoxia for 24 hours, the HIF-1alpha expression in myocardial cells was significantly increased. The LDH level in the culture medium was increased from (93.07 +/- 15.84) U x L(-1) to (750.77 +/- 181.51) U x L(-1) (P < 0.01). The intracellular GSH-Px activity was decreased from (46.96 +/- 8.36) U x mL(-1) to (27.13 +/- 4.76) U x mL(-1) (P < 0.05). Res 25, 50 and 75 micromol x L(-1) could dose-dependently inhibit the raising of the HIF-1alpha expression in myocardial cells induced by hypoxia. The LDH activities were decreased dose-dependently to (486.17 +/- 69.97), (189.43 +/- 32.07), (155.34 +/- 29.57) U x L(-1), respectively (P <0.05 or P <0.01). The GSH-Px activities were increased dose-dependently (33.55 +/- 6.34), (37.67 +/- 6.73), (41.44 +/- 7.91) U x mL(-1) (P < 0.05 or P < 0.01).


CONCLUSIONS
Res has a protective effect on neonatal rat cardiomyocyte lesion induced by hypoxia.
Huang
Xiulan
X
Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China. hxlcun@sina.com
Zhou
Qiulan
Q
Gu
Lili
L
Liu
Danni
D
Li
Zhiyong
Z
Liu
Qingshan
Q
Zhu
Dan
D
D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D013267:Stilbenes; D007770:L-Lactate Dehydrogenase; D000077185:Resveratrol