title: Opposite effects of Trichostatin A on activation of mast cells by different stimulants.
abstract: Mast cells (MCs) are activated upon stimulation via TLRs or FcepsilonRI, contributing to immune protection and/or leading to allergic diseases. In the present study, the effects of Trichostatin A (TSA) on the activation of MCs were analyzed with bone marrow-derived (BM) MCs. TSA increased the transcription and protein secretion of IL-6 in case of LPS-stimulation, in contrast to the suppressive effect on IgE-mediated activation of BMMCs. Chromatin immunoprecipitation assay showed IgE-mediated signaling-specific suppression of transcription factors recruitment to the IL-6 promoter. TSA-treatment inhibited nuclear translocation of NF-kappaB following IgE-mediated, but not LPS-induced activation in MCs.
authors.affiliation: Atopy Research Center, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Qing-hui
authors.initials: QH
authors.lastname: Wang
authors.forename: Chiharu
authors.initials: C
authors.lastname: Nishiyama
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nakano
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kanada
authors.forename: Mutsuko
authors.initials: M
authors.lastname: Hara
authors.forename: Nao
authors.initials: N
authors.lastname: Kitamura
authors.forename: Naomi
authors.initials: N
authors.lastname: Shimokawa
authors.forename: Chang-long
authors.initials: CL
authors.lastname: Lu
authors.forename: Hideoki
authors.initials: H
authors.lastname: Ogawa
authors.forename: Ko
authors.initials: K
authors.lastname: Okumura
mesh_terms: D006877:Hydroxamic Acids / Q000494:pharmacology*; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D006877:Hydroxamic Acids; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014157:Transcription Factors; C012589:trichostatin A