An integrated protein localization and interaction map for Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus.
The genome of Potato yellow dwarf virus (PYDV; Nucleorhabdovirus type species) was determined to be 12,875 nucleotides (nt). The antigenome is organized into seven open reading frames (ORFs) ordered 3'-N-X-P-Y-M-G-L-5', which likely encode the nucleocapsid, phospho, movement, matrix, glyco and RNA-dependent RNA polymerase proteins, respectively, except for X, which is of unknown function. The ORFs are flanked by a 3' leader RNA of 149 nt and a 5' trailer RNA of 97 nt, and are separated by conserved intergenic junctions. Phylogenetic analyses indicated that PYDV is closely related to other leafhopper-transmitted rhabdoviruses. Functional protein assays were used to determine the subcellular localization of PYDV proteins. Surprisingly, the M protein was able to induce the intranuclear accumulation of the inner nuclear membrane in the absence of any other viral protein. Finally, bimolecular fluorescence complementation was used to generate the most comprehensive protein interaction map for a plant-adapted rhabdovirus to date.
Bandyopadhyay
Anindya
A
Department of Plant Pathology, University of Kentucky, Lexington, KY, USA.
Kopperud
Kristin
K
Anderson
Gavin
G
Martin
Kathleen
K
Goodin
Michael
M
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D002467:Cell Nucleus / Q000737:chemistry; D016000:Cluster Analysis; D003593:Cytoplasm / Q000737:chemistry; D021901:DNA, Intergenic; D023061:Gene Order; D016679:Genome, Viral; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009685:Nuclear Envelope / Q000737:chemistry; D016366:Open Reading Frames; D010802:Phylogeny; D011485:Protein Binding; D025941:Protein Interaction Mapping; D012367:RNA, Viral / Q000235:genetics; D012209:Rhabdoviridae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D011198:Solanum tuberosum / Q000821:virology*; D014026:Nicotiana / Q000821:virology; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D021901:DNA, Intergenic; D012367:RNA, Viral; D014764:Viral Proteins