Saccharospirillum aestuarii sp. nov., isolated from tidal flat sediment, and an emended description of the genus Saccharospirillum.
A Gram-reaction-negative, chemoheterotrophic, non-motile, facultatively anaerobic, curved rod-shaped bacterial strain, IMCC4453(T), was isolated from tidal flat sediment and subjected to a taxonomic study using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IMCC4453(T) belonged to the genus Saccharospirillum, forming a robust clade with members of the genus, and was most closely related to the type strains of Saccharospirillum salsuginis (97.6  % similarity) and Saccharospirillum impatiens (95.9  %). The DNA-DNA relatedness value between strain IMCC4453(T) and S. salsuginis YIM-Y25(T) was 23-30  %. Differences in several physiological and biochemical characteristics between strain IMCC4453(T) and the two recognized species of the genus Saccharospirillum, together with phylogenetic and genomic distinctiveness, differentiated the novel strain from members of the genus Saccharospirillum. On the basis of the data from the present study, it is concluded that strain IMCC4453(T) represents a novel species of the genus Saccharospirillum, for which the name Saccharospirillum aestuarii sp. nov. is proposed. The type strain is IMCC4453(T) (=KCTC 22684(T)=KCCM 42930(T)=NBRC 105825(T)). An emended description of the genus Saccharospirillum is provided.
Choi
Ahyoung
A
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Oh
Hyun-Myung
HM
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Cho
Jang-Cheon
JC
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S