The immunotherapeutic effect of Fe3O4 nanoparticles as adjuvants on mice H22 live cancer.
The aim of this paper is to prepare Fe3O4 nanoparticles and study its immunotherapeutic effect as adjuvants on mice H22 live cancer. The Fe3O4 nanoparticles were prepared by chemical coprecipitation route. Transmission electron microscopy (TEM), X-ray diffraction (XRD), Energy Dispersive Analysis (EDS) were used to characterize Fe3O4 nanoparticles. The Fe3O4 nanoparticles were compared with the common alum adjuvants for its ability to induce immunity to inhibit tumor growth rate by prophylactic and therapeutic studies. Results indicated that Fe3O4 nanopaticles adsorbed autovaccine took great advantages over the common alum adjuvants after subcutaneous injection, raised the mass inhibitory rate of tumor, boosted the activity of cytotoxicity and enhanced the level of IFN-gamma cytokine. Thus, we concluded that Fe3O4 nanoparticles as adjuvants had great potential for enhancing anti-tumor immune response.
Liu
Hui
H
Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Southeast University, Nanjing, 210009, China.
Zhanl
Dongsheng
D
Du
Yiqun
Y
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D019496:Cancer Vaccines / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005290:Ferric Compounds / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use* / Q000648:ultrastructure; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology / Q000628:therapy*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology; D014961:X-Ray Diffraction
D000276:Adjuvants, Immunologic; D019496:Cancer Vaccines; D005290:Ferric Compounds; C000499:ferric oxide; D015847:Interleukin-4; D007371:Interferon-gamma