Evaluation of the Sysmex UF1000i flow cytometer for ruling out bacterial urinary tract infection.
BACKGROUND
Urine culture is one of the most frequently requested tests in microbiology, and it represents the gold standard for the diagnosis of UTIs. Considering the high prevalence of negative results and the long TAT of the culture test, the use of a rapid and reliable screening method is becoming more and more important, as it reduces the workload, the TAT of negative results, and above all, unnecessary antibiotic prescription.


METHODS
The Sysmex UF1000i is a new urine flow cytometry analyzer capable of quantifying urinary particles, including BACT, WBCs, and YLCs. To evaluate the analytical performance of the UF1000i as a method for ruling out UTIs, we examined 1349 urine samples and compared the UF1000i results with standard urine culture results.


RESULTS
With instrument cut-off values of 170BACTx10(6)/L and 150WBCsx10(6)/L, we obtained a sensitivity of 98.8%, a specificity of 76.5%, a NPV of 99.5%, and four false negative results (1.2%), avoiding the culture of 57.1% of samples.


CONCLUSIONS
The Sysmex UF1000i was capable of improving the efficiency of a routine microbiology laboratory by processing 100samples/h and providing negative results in a few minutes, thus reducing unnecessary testing with an acceptable number of false negative results. In addition, the preliminary evaluation of B_FSC and B_FLH parameters from bacteria histograms seems to be useful for the distinction of bacterial strains detected (Gram-negatives versus Gram-positives). In fact when B_FSC was less than 30 ch, it allowed the distinction of Gram-negative bacteria in 97% of the samples.
De Rosa
Rita
R
Microbiology and Virology Department, Azienda Ospedaliera S. Maria degli Angeli, Via Montereale 24, 33170 Pordenone, Italy. rita.derosa@aopn.fvg.it
Grosso
Shamanta
S
Bruschetta
Graziano
G
Avolio
Manuela
M
Stano
Paola
P
Modolo
Maria Luisa
ML
Camporese
Alessandro
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001424:Bacterial Infections / Q000175:diagnosis* / Q000652:urine; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000379:methods* / Q000592:standards; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012372:ROC Curve; D012015:Reference Standards; D013997:Time Factors; D016482:Urinalysis / Q000379:methods* / Q000592:standards; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology* / Q000652:urine; D055815:Young Adult