Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells.
Phosphatidylinositol 3-phosphate (PtdIns(3)P) is a phospholipid residing on early endosomes, where it is proposed to be involved in endosomal fusion. We synthesized membrane-permeant derivatives of PtdIns(3)P, including a caged version that is to our knowledge the first photoactivatable phosphoinositide derivative developed so far. In living cells, photoactivation of caged PtdIns(3)P induced rapid endosomal fusion in an EEA1-dependent fashion, thus providing in vivo evidence that PtdIns(3)P is a sufficient signal for driving this process.
Subramanian
Devaraj
D
European Molecular Biology Institute, Cell Biology and Biophysics Unit, Heidelberg, Germany.
Laketa
Vibor
V
Müller
Rainer
R
Tischer
Christian
C
Zarbakhsh
Sirus
S
Pepperkok
Rainer
R
Schultz
Carsten
C
D002459:Cell Fusion; D002463:Cell Membrane Permeability; D011992:Endosomes / Q000648:ultrastructure*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*
D018129:Phosphatidylinositol Phosphates; C055525:phosphatidylinositol 3-phosphate