Characterization of the GHR gene genetic variation in Chinese indigenous goat breeds.
The aim of the present work was to investigate single nucleotide polymorphism (SNP) of growth hormone receptor (GHR) gene exon 10, characterize the genetic variation in three Chinese indigenous goat breeds, and search for its potential association with cashmere traits. In this study, a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) protocol has been developed for rapid genotyping of the GHR gene in goats. One hundred seventy-eight goats from Liaoning Cashmere (96), Inner Mongolia White Cashmere (40), and Chengdu Grey (42) breeds in China were genotyped at GHR locus using the protocol developed. In all goat breeds investigated, a SNP in exon 10 of GHR gene has been identified by analyzing genomic DNA. The polymorphism consists of a single nucleotide substitution A → G, resulting in two alleles named, respectively, A and G based on the nucleotide at the position. The allele A was found to be more common in the animals investigated, and seems to be more consistent with cattle and zebu at this polymorphic site found in goats. The Hardy-Weinberg equilibrium of genotype distributions of GHR locus was verified in Liaoning Cashmere, and Inner Mongolia White Cashmere breeds. According to the classification of polymorphism information content (PIC), Chengdu Grey was less polymorphic than Liaoning Cashmere and Inner Mongolia White Cashmere breeds at this locus. The phylogenetic tree of different species based on the nucleotide sequences of GHR gene exon 10 is generally in agreement with the known species relationship. No significant association was found between the polymorphism revealed and the cashmere traits analyzed in present work.
Bai
W L
WL
College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110161, China. baiwenlin55@163.com
Zhou
C Y
CY
Ren
Y
Y
Yin
R H
RH
Jiang
W Q
WQ
Zhao
S J
SJ
Zhang
S C
SC
Zhang
B L
BL
Luo
G B
GB
Zhao
Z H
ZH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002417:Cattle; D002681:China; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006041:Goats / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011986:Receptors, Somatotropin / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D034561:Sheep, Domestic / Q000235:genetics
D011986:Receptors, Somatotropin