title: Discovery of a gene conferring multiple-aminoglycoside resistance in Escherichia coli.
abstract: Bovine-origin Escherichia coli isolates were tested for resistance phenotypes using a disk diffusion assay and for resistance genotypes using a DNA microarray. An isolate with gentamicin and amikacin resistance but with no corresponding genes detected yielded a 1,056-bp DNA sequence with the closest homologues for its inferred protein sequence among a family of 16S rRNA methyltransferase enzymes. These enzymes confer high-level aminoglycoside resistance and have only recently been described in Gram-negative bacteria.
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA. madavis@vetmed.wsu.edu
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Davis
authors.forename: Katherine N K
authors.initials: KN
authors.lastname: Baker
authors.forename: Lisa H
authors.initials: LH
authors.lastname: Orfe
authors.forename: Devendra H
authors.initials: DH
authors.lastname: Shah
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Besser
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Call
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D005798:Genes, Bacterial; D066298:In Vitro Techniques; D008780:Methyltransferases / Q000145:classification / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D012329:RNA, Bacterial / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000378:metabolism
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase