title: Liposomes for entrapping local anesthetics: a liposome electrokinetic chromatographic study.
abstract: Bupivacaine is a lipophilic, long-acting, amide class local anesthetic commonly used in clinical practice to provide local anesthesia during surgical procedures. Several cases of accidental overdose with cardiac arrest and death have been reported since bupivacaine was introduced to human use. Recent case reports have suggested that Intralipid (Fresenius Kabi) is an effective therapy for cardiac toxicity from high systemic concentrations of, e.g. bupivacaine, even though the mechanism behind the interaction is not fully clear yet. Our long-term aim is to develop a sensitive, efficient, and non-harmful lipid-based formulation to specifically trap harmful substances in vivo. In this study, the in vitro interaction of local anesthetics (bupivacaine, prilocaine, and lidocaine) with Intralipid or lipid vesicles containing phosphatidylglycerol, phosphatidylcholine, cardiolipin, cholesterol, and N-palmitoyl-D-erythro-sphingosine (ceramide) was determined by liposome electrokinetic chromatography. The interactions were evaluated by calculating the retention factors and distribution constants. Atomic force microscopy measurements were carried out to confirm that the interaction mechanism was solely due to interactions between the analytes and the moving pseudostationary phase and not by interactions with a stationary lipid phase adsorbed to the fused-silica wall. The heterogeneity of the liposomes was also studied by atomic force microscopy. The liposome electrokinetic chromatography results demonstrate that there is higher interaction between the drugs and negatively charged liposome dispersion than with the commercial Intralipid dispersion.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Finland.
authors.forename: Jana
authors.initials: J
authors.lastname: Lokajová
authors.forename: Jaana
authors.initials: J
authors.lastname: Laine
authors.forename: Esa
authors.initials: E
authors.lastname: Puukilainen
authors.forename: Mikko
authors.initials: M
authors.lastname: Ritala
authors.forename: Juha M
authors.initials: JM
authors.lastname: Holopainen
authors.forename: Susanne K
authors.initials: SK
authors.lastname: Wiedmer
mesh_terms: D000779:Anesthetics, Local / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000813:Anilides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D010087:Oxides; D010316:Particle Size; D017655:Silicon Compounds
chemical_list: D000779:Anesthetics, Local; D000813:Anilides; D008055:Lipids; D008081:Liposomes; D010087:Oxides; D017655:Silicon Compounds; C116473:silicon monoxide