[The influence of immunosuppression on vancomycin concentration in the rat allogenic lung transplant].
OBJECTIVE
To assess the influence of conventional immunosuppression on vancomycin concentration in the rat allogenic lung transplant basing on acute and hyperacute rejection models.


METHODS
Left lung allotransplantations were performed from Brown Norway donors to Fisher F 344 recipients in the acute rejection model (animals were sacrificed 5 days after transplantation), and from Brown Norway donors to Wistar recipients in the hyperacute rejection model (animals were sacrificed 2 days after transplantation). Immunosuppression (cyclosporin A 5 mg/kg b.w., aziathioprine 4 mg/kg b.w., methylprednisolone 4 mg/kg b.w.) was administered daily. Control rats received no immunosuppression and were sacrificed on day 2 or 5, respectively. Rejection grading was done on the basis of arterial pO2 and histology of the lung graft sample obtained at autopsy. A single 30 mg/kg b.w. dose ofvancomycin was injected intraperitoneally on day 2 or 5 depending on the model. Samples of blood and grafted lung were collected 30 min, 1 h, 2 h, 4 h, and 6 h from injection.


RESULTS
Arterial pO2 levels were significantly higher in the group with acute rejection and immunosuppression as compared with the control groups. Histology revealed attenuated rejection in the immunosuppression groups. Vancomycin concentration in the transplanted lung and the lung graft to plasma vancomycin concentration ratio did not differ in the immunosuppression and control groups.


CONCLUSIONS
Immunosuppression has no influence on vancomycin concentration in the transplanted lung.
Pieróg
Jarosław
J
Oddzial Kliniczny Chirurgii Klatki Piersiowej Pomorskiej Akademii Medycznej w Szczecinie ul. Sokolowskiego 11, 70-891 Szczecin.
Kubisa
Bartosz
B
Droździk
Marek
M
Wójcik
Janusz
J
Wojtyś
Małgorzata
M
Bielewicz
Michal
M
Pankowski
Juliusz
J
Witkowska
Dominika
D
Safranow
Krzysztof
K
Grodzki
Tomasz
T
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D001379:Azathioprine / Q000008:administration & dosage; D016572:Cyclosporine / Q000008:administration & dosage; D006084:Graft Rejection / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D008168:Lung / Q000737:chemistry*; D016040:Lung Transplantation / Q000276:immunology* / Q000379:methods / Q000473:pathology*; D008775:Methylprednisolone / Q000008:administration & dosage; D010100:Oxygen / Q000097:blood; D051381:Rats; D011916:Rats, Inbred F344; D014184:Transplantation, Homologous; D014640:Vancomycin / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics
D000900:Anti-Bacterial Agents; D007166:Immunosuppressive Agents; D014640:Vancomycin; D016572:Cyclosporine; D001379:Azathioprine; D010100:Oxygen; D008775:Methylprednisolone