The effects of di-n-butyl phthalate on the germ cells of laboratory mice.
Phthalate are found in the environmental samples due to their wide use in the industry as plasticizers. Di-n-butylphthalate (DBP) is mainly used in nitrocellulose and polyvinyl acetate products as well as in personal-care products. This study was performed to investigate the influence of exposure to DBP on the quantity and quality (motility, morphology) and DNA damage (induction of micronuclei and DNA strand breaks) of male mice gametes. The estimation of DBP residues was also done. Eight weeks exposure to DBP (500 mg/kg bw and 2000 mg/kg bw) did not significantly affect testes and epididymes weights as well as sperm count. DBP clearly diminished sperm motility, enhanced frequency of abnormal sperm heads and not significantly increased DNA strand breaks in germ cells as well as frequency of micronuclei in spermatids. There were no bioacumulation of DBP in mice. Results suggest that DBP may affect the male mice germ cells.
Dobrzyńska
Małgorzata M
MM
Department of Radiation Protection and Radiobiology National Institute of Public Health - National Institute of Hygiene, Warsaw. mdobrzynska@pzh.gov.pl
Tyrkiel
Ewa J
EJ
Hernik
Agnieszka
A
Derezińska
Edyta
E
Góralczyk
Katarzyna
K
Ludwicki
Jan K
JK
D000818:Animals; D004249:DNA Damage; D004785:Environmental Pollutants / Q000633:toxicity*; D004822:Epididymis / Q000187:drug effects; D008297:Male; D051379:Mice; D010795:Phthalic Acids / Q000633:toxicity*; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D013723:Teratogens / Q000633:toxicity*; D013737:Testis / Q000187:drug effects
D004785:Environmental Pollutants; D010795:Phthalic Acids; D013723:Teratogens; C027561:butylbenzyl phthalate