title: Patterned poly(2-hydroxyethyl methacrylate) brushes on silicon surfaces behave as "tentacles" to capture ferritin from aqueous solution.
abstract: We have used a very large scale integration process to generate well-defined patterns of polymerized 2-hydroxyethyl methacrylate (HEMA) on patterned Si(100) surfaces. An atom transfer radical polymerization initiator covalently bonded to the patterned surface was employed for the graft polymerization of HEMA to prepare the poly(2-hydroxyethyl methacrylate) (PHEMA) brushes. After immersing wafers presenting lines of these polymers in water and cyclohexane, we observed brush- and mushroom-like regions, respectively, for the PHEMA brushes, with various pattern resolutions. The PHEMA brushes behaved as "tentacles" that captured ferritin complexes from aqueous solution through entanglement between the brushes and the ferritin proteins, whose ferritins were trapped due to the collapsing of the PHEMA. Using high-resolution scanning electron microscopy, we observed patterned ferritin iron cores on the Si surface after thermal removal of the patterned PHEMA brushes and ferritin protein sheaths.
authors.affiliation: Department of Polymer Engineering, National Taiwan University of Science and Technology, 43, Sec 4, Keelung Rd, Taipei 106, Taiwan. jkchen@mail.ntust.edu.tw
authors.forename: Jem-Kun
authors.initials: JK
authors.lastname: Chen
authors.forename: Zong-Yan
authors.initials: ZY
authors.lastname: Chen
authors.forename: Han-Ching
authors.initials: HC
authors.lastname: Lin
authors.forename: Po-Da
authors.initials: PD
authors.lastname: Hong
authors.forename: Feng-Chih
authors.initials: FC
authors.lastname: Chang
mesh_terms: D000327:Adsorption; D003510:Cyclohexanes / Q000737:chemistry; D005293:Ferritins / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008956:Models, Chemical; D011506:Proteins / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D003510:Cyclohexanes; D008689:Methacrylates; D011506:Proteins; D012997:Solvents; D014867:Water; C506365:Cyclohexane; C005044:hydroxyethyl methacrylate; D005293:Ferritins; D012825:Silicon