Molecular characterization of two Chinese isolates of Beet western yellows virus infecting sugar beet.
Beet western yellows virus (BWYV) has previously been reported as an agent of sugar beet yellowing disease in China. In this article, the complete genomic RNA sequences of two Chinese BWYV isolates infecting beet from Inner Mongolia (BWYV-IM) and Gansu (BWYV-GS) were determined and compared with three beet poleroviruses (BMYV, BChV and BWYV-US) and other non-beet-infecting poleroviruses. The genomes of the two isolates were 5,668 nt in length, and had almost the same genomic organization and characteristics as BWYV-US. The full length of BWYV-IM shared nucleotide sequence identities of 97.4, 86.6, 64.4 and 70.8% with BWYV-GS, BWYV-US, BChV and BMYV, respectively. Further sequence analysis indicated that the Chinese BWYV isolates were more closely related to BWYV-US; however, the identity of any gene product between the Chinese isolates and BWYV-US was <90%. Therefore, on the basis of genome sequence, we propose that these Chinese isolates are a distinct strain of BWYV that infect sugar beet. In addition, recombinant detection analysis revealed that BWYV-IM might be a recombinant virus.
Xiang
Hai-Ying
HY
State Key Laboratory for Agrobiotechnology and Agriculture Ministry Laboratory for Plant Pathology, China Agricultural University, Beijing, 100193, China.
Dong
Shu-Wei
SW
Zhang
Hui-Zhong
HZ
Wang
Wei-Lin
WL
Li
Min-Quan
MQ
Han
Cheng-Gui
CG
Li
Da-Wei
DW
Yu
Jia-Lin
JL
D001483:Base Sequence; D027461:Beta vulgaris / Q000821:virology*; D002681:China; D018745:Genome, Plant; D017793:Luteovirus / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*