A DEAD-box protein, AtRH36, is essential for female gametophyte development and is involved in rRNA biogenesis in Arabidopsis.
DEAD-box RNA helicases are involved in RNA metabolism, including pre-mRNA splicing, ribosome biogenesis, RNA decay and gene expression. In this study, we identified a homolog of the RH36 gene, AtRH36, which encodes a DEAD-box protein in Arabidopsis thaliana. The gene was expressed ubiquitously throughout the plant. The AtRH36 fused to green fluorescent protein was localized in the nucleus. Homozygosity for the Arabidopsis atrh36 mutants, atrh36-1 and atrh36-2, could not be obtained. Progeny of selfed Arabidopsis atrh36 heterozygote plants were obtained at a heterozygote to wild-type ratio of 1 : 1, which suggested that the AtRH36 gene was involved in gametogenesis. Therefore, we performed a reciprocal cross to determine whether AtRH36 was involved in female gametophyte development. Female gametogenesis was delayed in atrh36-1, and asynchronous development of the female gametophytes was found within a single pistil. Knock-down of AtRH36 gave a pleiotropic phenotype and led to the accumulation of unprocessed 18S pre-rRNA. These results suggest that AtRH36 is essential for mitotic division during female gametogenesis and plays an important role in rRNA biogenesis in Arabidopsis.
Huang
Chun-Kai
CK
Department of Life Science, National Central University, Jhongli City, Taoyuan County 320, Taiwan, ROC.
Huang
Li-Fen
LF
Huang
Jin-Ji
JJ
Wu
Shaw-Jye
SJ
Yeh
Ching-Hui
CH
Lu
Chung-An
CA
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003433:Crosses, Genetic; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D056130:Gametogenesis, Plant; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D016254:Mutagenesis, Insertional; D009154:Mutation; D056771:Ovule / Q000254:growth & development*; D011401:Promoter Regions, Genetic; D018749:RNA, Plant / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis*
D029681:Arabidopsis Proteins; D018749:RNA, Plant; D012335:RNA, Ribosomal; D053487:DEAD-box RNA Helicases