A newly designed cell-permeable SNARF derivative as an effective intracellular pH indicator.
We have successfully developed a new cell-permeable SNARF derivative that is activated inside the cell by ester hydrolysis and is notable for having reduced background fluorescence.
Nakata
Eiji
E
Department of Life System, Institute of Technology and Science, Graduate School, The University of Tokushima, Minamijosanjimacho 2, Tokushima 770-8506, Japan. nakata@bio.tokushima-u.ac.jp
Yukimachi
Yoshihiro
Y
Nazumi
Yoshijiro
Y
Uto
Yoshihiro
Y
Maezawa
Hiroshi
H
Hashimoto
Toshihiro
T
Okamoto
Yasuko
Y
Hori
Hitoshi
H
D000818:Animals; D001578:Benzopyrans / Q000138:chemical synthesis / Q000737:chemistry*; D002460:Cell Line; D002463:Cell Membrane Permeability; D006224:Cricetinae; D003412:Cricetulus; D004950:Esterases / Q000737:chemistry / Q000378:metabolism; D004952:Esters / Q000737:chemistry; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007202:Indicators and Reagents / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D009284:Naphthols / Q000138:chemical synthesis / Q000737:chemistry*; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*
D001578:Benzopyrans; D004952:Esters; D007202:Indicators and Reagents; D009284:Naphthols; D012235:Rhodamines; C091666:seminaphthorhodaminefluoride; D004950:Esterases