title: Microscopic mammalian retinal pigment epithelium lesions induce widespread proliferation with differences in magnitude between center and periphery.
abstract: OBJECTIVE
  The vertebrate retina develops from the center to the periphery. In amphibians and fish the retinal margin continues to proliferate throughout life, resulting in retinal expansion. This does not happen in mammals. However, some mammalian peripheral retinal pigment epithelial (RPE) cells continue to divide, perhaps as a vestige of this mechanism. The RPE cells are adjacent to the ciliary margin, a known stem cell source. Here we test the hypothesis that peripheral RPE is fundamentally different from central RPE by challenging different regions with microscopic laser burns and charting differential responses in terms of levels of proliferation and the regions over which this proliferation occurs.
  
  
  METHODS
  Microscopic RPE lesions were undertaken in rats at different eccentricities and the tissue stained for proliferative markers Ki67 and bromodeoxyuridine (BrdU) and the remodeling metalloproteinase marker 2 (MMP2).
  
  
  RESULTS
  All lesions produced local RPE proliferation and tissue remodeling. Significantly more mitosis resulted from peripheral than central lesions. Unexpectedly, single lesions also resulted in RPE cells proliferating across the entire retina. Their number did not increase linearly with lesion number, indicating that they may be a specific population. All lesions repaired and formed apparently normal relations with the neural retina. Repaired RPE was albino.
  
  
  CONCLUSIONS
  These results highlight regional RPE differences, revealing an enhanced peripheral repair capacity. Further, all lesions have a marked impact on both local and distant RPE cells, demonstrating a pan retinal signaling mechanism triggering proliferation across the tissue plane. The RPE cells may represent a distinct population as their number did not increase with multiple lesions. The fact that repairing cells were hypopigmented is of interest because reduced pigment is associated with enhanced proliferative capacities in the developing neural retina.
authors.forename: Peter Lundh
authors.initials: PL
authors.lastname: von Leithner
authors.forename: Coziana
authors.initials: C
authors.lastname: Ciurtin
authors.forename: Glen
authors.initials: G
authors.lastname: Jeffery
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count; D002453:Cell Cycle; D049109:Cell Proliferation; D005453:Fluorescence; D019394:Ki-67 Antigen / Q000378:metabolism; D008028:Light Coagulation; D008322:Mammals / Q000378:metabolism*; D051381:Rats; D055213:Retinal Pigment Epithelium / Q000473:pathology*; D014945:Wound Healing
chemical_list: D019394:Ki-67 Antigen; D001973:Bromodeoxyuridine