Mechanism for alpha-MnO2 nanowire-induced cytotoxicity in Hela cells.
Alpha-Manganese dioxide (alpha-MnO2) nanowires are used as electrode materials to significantly enhance the performance of lithium batteries. In this study, we investigate the nanotoxicity of alpha-MnO2 nanowires toward Hela cells. The alpha-MnO2 nanowires, which were successfully synthesized using the hydrothermal approach, can induce cytotoxicity dose-dependently in Hela cells. The accumulation of reactive oxygen species (ROS) and depletion of glutathione (GSH) are also observed in the nanowire-treated cells. In addition, comet assays and cell nucleus morphology show that both DNA damage and cell apoptosis occur in the nanowires exposure group. Based on these results, a mechanism for alpha-MnO2 nanowire-induced cytotoxicity in Hela cells, which involves the accumulation of ROS, formation of oxidative stress, DNA oxidative damage and cell apoptosis, is proposed. This investigation may provide a fundamental insight to understand the nanotoxicity of wire-shaped nanomaterials.
Li
Yan
Y
College of Life Science, Central China Normal University, Wuhan 430079, China.
Tian
Xike
X
Lu
Zhisong
Z
Yang
Chao
C
Yang
Guangtao
G
Zhou
Xiaochong
X
Yao
Hanchao
H
Zhu
Zhihong
Z
Xi
Zhuge
Z
Yang
Xu
X
D017209:Apoptosis; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D017895:Manganese Compounds / Q000737:chemistry / Q000494:pharmacology*; D053770:Nanowires / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis
D017895:Manganese Compounds; D010087:Oxides; D017382:Reactive Oxygen Species; D005978:Glutathione; C016552:manganese dioxide