title: Superoxide dismutase, protease and lipase expression in clinical isolates of Staphylococcus aureus: a tool for antimicrobial drug discovery.
abstract: The rising incidents of invasive infections due to multidrug resistant Staphylococcus aureus necessitate the exploration of newer targets for development of antibiotics. Pathogenicity of S. aureus is attributed to a wide range of virulence factors. The aim of this study was to screen the production of three virulence factors viz. extracellular protease, extracellular lipase and superoxide dismutase in human pathogenic strains of S. aureus for development of a test panel which could aid in screening of natural products of plant and microbial origin. 27 clinical isolates were compared for their enzyme expression profiles of which eight were finally selected. Sau G5 was the only protease producing organism selected in the test panel, while Sau G3 and Sau G9 were best SOD producers and Sau G16, Sau G18, Sau G22, Sau A5 and Sau A2 exhibited highest expression among different groups of clinical staphylococci.
authors.affiliation: Department of Biotechnology and Environmental Sciences, Thapar University, Patiala, 147004 Punjab, India. sanjaibiotech@yahoo.com
authors.forename: Sanjai
authors.initials: S
authors.lastname: Saxena
authors.forename: Charu
authors.initials: C
authors.lastname: Gomber
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001688:Biological Products / Q000494:pharmacology; D055808:Drug Discovery / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans; D008049:Lipase / Q000096:biosynthesis / Q000235:genetics; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000235:genetics; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000472:pathogenicity; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics; D037521:Virulence Factors / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001688:Biological Products; D037521:Virulence Factors; D013482:Superoxide Dismutase; D008049:Lipase; D010447:Peptide Hydrolases