In situ fluorescence imaging of myelination.
We describe a novel fluorescent dye, 3-(4-aminophenyl)-2H-chromen-2-one (termed case myelin compound or CMC), that can be used for in situ fluorescent imaging of myelin in the vertebrate nervous system. When administered via intravenous injection into the tail vein, CMC selectively stained large bundles of myelinated fibers in both the central nervous system (CNS) and the peripheral nervous system (PNS). In the CNS, CMC readily entered the brain and selectively localized in myelinated regions such as the corpus callosum and cerebellum. CMC also selectively stained myelinated nerves in the PNS. The staining patterns of CMC in a hypermyelinated mouse model were consistent with immunohistochemical staining. Similar to immunohistochemical staining, CMC selectively bound to myelin sheaths present in the white matter tracts. Unlike CMC, conventional antibody staining for myelin basic protein also stained oligodendrocyte cytoplasm in the striatum as well as granule layers in the cerebellum. In vivo application of CMC was also demonstrated by fluorescence imaging of myelinated nerves in the PNS.
Wang
Changning
C
Case Center for Imaging Research, Department of Radiology, Case Western Reserve University, Cleveland, OH 44106, USA.
Popescu
Daniela C
DC
Wu
Chunying
C
Zhu
Junqing
J
Macklin
Wendy
W
Wang
Yanming
Y
D000818:Animals; D002490:Central Nervous System / Q000737:chemistry; D002867:Chromones / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009186:Myelin Sheath / Q000737:chemistry / Q000502:physiology*; D017933:Peripheral Nervous System / Q000737:chemistry
C550732:3-(4-aminophenyl)-2H-chromen-2-one; D002867:Chromones; D003374:Coumarins; D005456:Fluorescent Dyes