title: Preparation and characterization of a magnetic and optical dual-modality molecular probe.
abstract: Multi-modality imaging probes combine the advantages of individual imaging techniques to yield highly detailed anatomic and molecular information in living organisms. Herein, we report the synthesis and characterization of a dual-modality nanoprobe that couples the magnetic properties of ultrasmall superparamagnetic iron oxide nanoparticles (USPIOs) with the near infrared fluorescence of Cy5.5. The fluorophore is encapsulated in a biocompatible shell of silica surrounding the iron oxide core for a final diameter of approximately 17 nm. This silica-coated iron oxide nanoparticle (SCION) has been analyzed by transmission electron microscopy, dynamic light scattering, and superconducting quantum interference device (SQUID). The particle demonstrates a strong negative surface charge and maintains colloidal stability in the physiological pH range. Magnetic hysteresis analysis confirms superparamagnetic properties that could be manipulated for thermotherapy. The viability of primary human monocytes, T cells, and B cells incubated with the particle has been examined in vitro. In vivo analysis of agent leakage into subcutaneous A431 tumors in mice was also conducted. This particle has been designed for diagnostic application with magnetic resonance and fluorescence imaging, and has future potential to serve as a heat-sensitive targeted drug delivery platform.
authors.affiliation: Radioimmune and Inorganic Chemistry Section, Radiation Oncology Branch, NCI, NIH, Bethesda, MD 20892, USA. Bumba@mail.nih.gov
authors.forename: A
authors.initials: A
authors.lastname: Bumb
authors.forename: C A S
authors.initials: CA
authors.lastname: Regino
authors.forename: M R
authors.initials: MR
authors.lastname: Perkins
authors.forename: M
authors.initials: M
authors.lastname: Bernardo
authors.forename: M
authors.initials: M
authors.lastname: Ogawa
authors.forename: L
authors.initials: L
authors.lastname: Fugger
authors.forename: P L
authors.initials: PL
authors.lastname: Choyke
authors.forename: P J
authors.initials: PJ
authors.lastname: Dobson
authors.forename: M W
authors.initials: MW
authors.lastname: Brechbiel
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D003952:Diagnostic Imaging / Q000379:methods*; D016503:Drug Delivery Systems; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D015336:Molecular Probe Techniques; D011437:Propylamines; D012821:Silanes
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005290:Ferric Compounds; D011437:Propylamines; D012821:Silanes; C000499:ferric oxide; C477625:amino-propyl-triethoxysilane