title: [Studies on IFN-gamma-mediated reversion of CIK killing sensitivity to edited human lung cancer A549 cells].
abstract: OBJECTIVE
  To explore IFN-gamma-mediated reversion of CIK killing sensitivity to immunoedited lung cancer A549 cells.
  
  
  METHODS
  RT-PCR and MTT methods were used to detect the effect on MICA mRNA expression induced by IFN-gamma in the edited A549 cells and the change of CIK killing sensitivity to A549 cells, respectively.
  
  
  RESULTS
  Low expression of cell surface MICA and low killing sensitivity of CIKs were observed in edited A549 cells. IFN-gamma could significantly increase MICA mRNA expression in the edited A549 cells and improve CIK cell cytotoxicity.
  
  
  CONCLUSIONS
  IFN-gamma could reverse CIK killing sensitivity to the edited A549 cells by enhancing the MICA mRNA expression.
authors.affiliation: Laboratory Center of the Affiliated Tumor Hospital of Guangzhou Medical College, Guangzhou 510095, China. jifangliu@hotmail.com
authors.forename: Ji-fang
authors.initials: JF
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Yan-chao
authors.initials: YC
authors.lastname: Qi
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Min-ying
authors.initials: MY
authors.lastname: Lu
authors.forename: Dong-xiao
authors.initials: DX
authors.lastname: Pan
authors.forename: Hong-zhuo
authors.initials: HZ
authors.lastname: Shen
mesh_terms: D045744:Cell Line, Tumor; D055612:Cytokine-Induced Killer Cells / Q000187:drug effects* / Q000276:immunology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000473:pathology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; D012333:RNA, Messenger; D007371:Interferon-gamma