title: Resistant starch, fermented resistant starch, and short-chain fatty acids reduce intestinal fat deposition in Caenorhabditis elegans.
abstract: Obesity is a growing global public health dilemma. The objective of this project is to develop and validate a screening mechanism for bioactive compounds that may reduce body fat and promote health. Resistant starch (RS) reduces body fat in rodents. Amylose starch that has a high content of RS, endogenous compounds obtained from the ceca of amylose starch fed mice (fermented RS), and individual short-chain fatty acids (SCFA) were tested. The Caenorhabditis elegans model and Nile red staining were selected to determine the intestinal fat deposition response to bioactive components. The fluorescence intensity of Nile red was reduced to 76.5% (amylose starch), 78.8% (fermented RS), 63.6% (butyrate), or 28-80% (SCFAs) of controls, respectively (P < 0.001). The reduced intestinal fat deposition suggests reduced food intake or increased energy expenditure. C. elegans is a practical animal model to screen for bioactive compounds that may prevent or treat obesity.
authors.affiliation: Veterinary Science Department, LSU AgCenter, Louisiana StateUniversity, Baton Rouge, Louisiana 70803, USA. zzheng@agcenter.lsu.edu
authors.forename: Jolene
authors.initials: J
authors.lastname: Zheng
authors.forename: Fred
authors.initials: F
authors.lastname: Enright
authors.forename: Michael
authors.initials: M
authors.lastname: Keenan
authors.forename: John
authors.initials: J
authors.lastname: Finley
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Jianping
authors.initials: J
authors.lastname: Ye
authors.forename: Frank
authors.initials: F
authors.lastname: Greenway
authors.forename: Reshani N
authors.initials: RN
authors.lastname: Senevirathne
authors.forename: Chris R
authors.initials: CR
authors.lastname: Gissendanner
authors.forename: Rosaly
authors.initials: R
authors.lastname: Manaois
authors.forename: Alfredo
authors.initials: A
authors.lastname: Prudente
authors.forename: Joan M
authors.initials: JM
authors.lastname: King
authors.forename: Roy
authors.initials: R
authors.lastname: Martin
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D005223:Fats / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005285:Fermentation; D007413:Intestinal Mucosa / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*
chemical_list: D005223:Fats; D005227:Fatty Acids; D013213:Starch