title: Production of recombinant human granulocyte macrophage colony-stimulating factor from silkworm Bombyx mori Bm5 cells.
abstract: Using silkworm Bombyx mori Bm5 cells, we established a stable cell line expressing the human granulocyte macrophage colony-stimulating factor (hGM-CSF), which gets its name from the Bm5-hGM-CSF cell in which the glycoprotein of the hGM-CSF is secreted in the cell culture supernatant (CCS). It was demonstrated that secreted hGM-CSF had in vivo biological activity and the white blood cell (WBC) value increased two times that of the control. We expect to produce useful human recombinant glycoproteins from silkworm cultured cells for a low price and a large quantity.
authors.affiliation: Department of Anatomy, School of Medicine, Chungnam National University, Taejon 301-747, Korea.
authors.forename: Cho-Yi
authors.initials: CY
authors.lastname: Jin
authors.forename: Song-Yi
authors.initials: SY
authors.lastname: Han
authors.forename: Kisang
authors.initials: K
authors.lastname: Kwon
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Yun
authors.forename: Seok Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Tae Won
authors.initials: TW
authors.lastname: Goo
authors.forename: Seung-Whan
authors.initials: SW
authors.lastname: Kim
authors.forename: Kweon
authors.initials: K
authors.lastname: Yu
authors.forename: O-Yu
authors.initials: OY
authors.lastname: Kwon
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002460:Cell Line; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor