Effect of inoculated corn silage enriched with sunflower oil on rumen fermentation and lipid metabolism in an artificial rumen (RUSITEC).
BACKGROUND
Some rumen isolates are able to produce conjugated linoleic acid (CLA) from linoleic acid (LA) in vitro. Effects of providing diets containing corn silage (CS) and lucerne hay to an artificial rumen (RUSITEC) in which the corn was not inoculated (CS), or inoculated with Lactobacillus plantarum CCM 4000 (CS + LP), Lactobacillus fermentum LF2 (CS + LF) or Enterococcus faecium CCM 4231 (CS + EF) and supplied with sunflower oil (SO; 30 g kg(-1); w/w) on rumen metabolism were examined.


RESULTS
The SO affected the outputs of all fatty acids. TVA output of uninoculated CS with SO was lower as compared to inoculated CS. The interaction of the CS x SO in the daily output of TVA was detected (P < 0.001). The biohydrogenation of oleic, linoleic, alpha-linolenic and total fatty acids was influenced by SO (P < 0.05 and P < 0.001).


CONCLUSIONS
Inoculated silage induces changes in the rumen metabolism which might be related to differences observed in the extent of rumen BH of PUFA in RUSITEC. SO supplementation might positively enhances the production of some rumen intermediates; however, relationships between inoculated silages and oil supplementation can be presumed in the daily production of trans-vaccenic acid.
Jalc
Dusan
D
Institute of Animal Physiology, Slovak Academy of Sciences, Soltésovej 4-6, 040 01 Kosice, Slovak Republic. jalcd@saske.sk
Váradyová
Zora
Z
Lauková
Andrea
A
D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism / Q000494:pharmacology; D016984:Enterococcus faecium / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism / Q000494:pharmacology; D005285:Fermentation; D006865:Hydrogenation; D048191:Lactiplantibacillus plantarum / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D000455:Medicago sativa; D023421:Models, Animal; D009829:Oleic Acids / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism / Q000494:pharmacology; D012417:Rumen / Q000378:metabolism* / Q000382:microbiology; D012820:Silage / Q000382:microbiology*; D000074242:Sunflower Oil; D003313:Zea mays / Q000382:microbiology*
D004041:Dietary Fats; D005227:Fatty Acids; D009829:Oleic Acids; D010938:Plant Oils; D000074242:Sunflower Oil; C050413:11-octadecenoic acid