title: Effect of gamma-irradiation on the biology and ultrastructure of haemocytes of greater wax moth, Galleria mellonella (L.) (Lepidoptera: Galleridae).
abstract: This study was carried out on fully grown pupae of greater wax moth, Galleria mellonella L., gamma-irradiated to 100, 150, 300 and 400Gy. The four doses given to male parents in the F(1) generation decreased the average number of eggs per mated female, the percentage of egg hatching and the percentage of mating in both the male and female lines; the effects increased with the dose. Dose dependence of the reduction in the fecundity and the percentage of egg hatching among the female line pairings (female descendants of irradiated parental male pupae) was more significant than among the male line pairings (male descendants of irradiated parental male pupae). We also examined morphological changes in the irradiated blood cells using transmission electron microscopy (TEM). Vacuolization of the cytoplasm, disorganization and swelling of mitochondria were found.
authors.affiliation: Biological Applications Department, Nuclear Research Center, Atomic Energy Authority, Cairo, Egypt.
authors.forename: Eman M S
authors.initials: EM
authors.lastname: El-Kholy
authors.forename: Nahla M
authors.initials: NM
authors.lastname: Abd El-Aziz
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006434:Hemocytes / Q000528:radiation effects* / Q000648:ultrastructure*; D008297:Male; D018811:Maternal Exposure; D009036:Moths / Q000528:radiation effects* / Q000648:ultrastructure*; D018812:Paternal Exposure; D011679:Pupa / Q000528:radiation effects / Q000648:ultrastructure