title: Distinct functional roles of peroxiredoxin isozymes and glutathione peroxidase from fission yeast, Schizosaccharomyces pombe.
abstract: To investigate the differences in the functional roles of peroxiredoxins (Prxs) and glutathione peroxidase (GPx) of Schizosaccharomyces pombe, we examined the peroxidase and molecular chaperone properties of the recombinant proteins. TPx (thioredoxin peroxidase) exhibited a capacity for peroxide reduction with the thioredoxin system. GPx also showed thioreoxin-dependent peroxidase activity rather than GPx activity. The peroxidase activity of BCP (bacterioferritin comigratory protein) was similar to that of TPx. However, peroxidase activity was not observed for PMP20 (peroxisomal membrane protein 20). TPx, PMP20, and GPx inhibited thermal aggregation of citrate synthase at 43(o)C, but BCP failed to inhibit the aggregation. The chaperone activities of PMP20 and GPx were weaker than that of TPx. The peroxidase and chaperone properties of TPx, BCP, and GPx of the fission yeast are similar to those of Saccharomyces cerevisiae. The fission yeast PMP20 without thioredoxin-dependent peroxidase activity may act as a molecular chaperone.
authors.affiliation: Department of Food and Nutrition, Chonnam National University, Gwangju, Korea.
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Mi-Ae
authors.initials: MA
authors.lastname: Bang
authors.forename: Songmi
authors.initials: S
authors.lastname: Lee
authors.forename: Ho Zoon
authors.initials: HZ
authors.lastname: Chae
authors.forename: Kanghwa
authors.initials: K
authors.lastname: Kim
mesh_terms: D002950:Citrate (si)-Synthase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D011994:Recombinant Proteins; D029702:Schizosaccharomyces pombe Proteins; D054464:Peroxiredoxins; C540496:SPBC1773.02c protein, S pombe; D005979:Glutathione Peroxidase; D002950:Citrate (si)-Synthase