Effects of scaling and root planing and subantimicrobial dose doxycycline on gingival crevicular fluid levels of matrix metalloproteinase-8, -13 and serum levels of HsCRP in patients with chronic periodontitis.
BACKGROUND
This study evaluates the efficacy of subantimicrobial dose doxycycline (SDD) in conjunction with scaling and root planing (SRP) on gingival crevicular fluid (GCF) levels of matrix metalloproteinase (MMP)-8 and -13 and on serum levels of high-sensitivity C-reactive protein (HsCRP) in patients with chronic periodontitis (CP).


METHODS
A total of 41 patients with CP and 17 healthy individuals were included in this randomized controlled trial. CP patients were randomly distributed into two groups. Study groups were established as Group I with SRP+placebo, Group II with SRP+SDD, and Group III as control. All CP patients received two regimens of SRP and Group II patients also received SDD for 6 weeks. At baseline and 6 weeks, GCF and blood were collected and clinical indices were recorded. The HsCRP level was assayed in the plasma on a nephelometer. The GCF levels of MMPs were assayed by an enzyme-linked immunosorbent assay.


RESULTS
Statistically significant differences in plaque index, gingival index (GI), probing depth (PD), GCF volumes, GCF MMP levels, and serum levels of HsCRP between pre-treatment and post-treatment were noted in both groups. Between groups there was a statistically significant decrease in PD, GI, and GCF levels of MMP-8 favoring the group receiving SDD adjunctive to SRP (P <0.05).


CONCLUSIONS
In this study, greater improvement was detected for PD, GI, and GCF levels of MMP-8 when using SRP+SDD compared to SRP+placebo.
Tüter
Gülay
G
Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Turkey. gulay@gazi.edu.tr
Serdar
Muhittin
M
Kurtiş
Bülent
B
Walker
Stephen G
SG
Atak
Ayşegül
A
Toyman
Utku
U
Pinar
Selin
S
Aykan
Tuba
T
D000328:Adult; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D002097:C-Reactive Protein / Q000032:analysis* / Q000187:drug effects; D055113:Chronic Periodontitis / Q000097:blood / Q000201:enzymology / Q000628:therapy*; D003774:Dental Plaque Index; D012534:Dental Scaling; D004318:Doxycycline / Q000008:administration & dosage*; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000032:analysis* / Q000187:drug effects; D020784:Matrix Metalloproteinase 8 / Q000032:analysis* / Q000187:drug effects; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000628:therapy; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000628:therapy; D010919:Placebos; D016745:Root Planing; D016896:Treatment Outcome
D000900:Anti-Bacterial Agents; D010919:Placebos; D002097:C-Reactive Protein; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8; D004318:Doxycycline