Low temperature photoacoustic spectroscopy of the purple membrane of Halobacterium halobium.
The photoacoustic spectra of light- and dark-adapted bacteriorhodopsin in aqueous and mixed water-glyc-erol (1:2) suspensions are studied in the 90-300 K temperature range. Photochemical transients are observed and interpreted in terms of the known photocycles of trans- and 13-cis-bacteriorhodopsin. The spectra differ from those obtained by low temperature optical absorption spectroscopy of glassy glycerol-water suspensions.
Bechthold
P S
PS
Kohl
K D
KD
Sperling
W
W