title: Glycoconjugate histochemistry in the small and large intestine of normal and Solanum glaucophyllum-intoxicated rabbits.
abstract: Vitamin D participates in mineral homeostasis, immunomodulation, cell growth and differentiation. The leaves of Solanum glaucophyllum contain high levels of 1,25-dihydroxyvitamin D3 as glycoside derivatives and their chronic ingestion generates a hypervitaminosis D-like state. We analyzed changes on carbohydrate expression as a cell differentiation indicator on samples of the small and large intestine of S. glaucophyllum-intoxicated rabbits, using conventional and lectin histochemistry. Male New Zealand white rabbits were intoxicated with S. glaucophyllum during two or four weeks and killed the day after. A group of animals ("possibly recovered group") were intoxicated during 15 days and killed at day 45 of the beginning of the experiment. We found changes in the lectin binding pattern in the small and large intestine of the intoxicated rabbits. Some of these changes were reverted in the possibly recovered group. Vitamin D could be a new regulator factor of the intestinal glycosylation process.
authors.affiliation: Institute of Pathology, Prof. Dr. Bernardo Epstein, School of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina. carozanuzzi@fcv.unlp.edu.ar
authors.forename: C N
authors.initials: CN
authors.lastname: Zanuzzi
authors.forename: C G
authors.initials: CG
authors.lastname: Barbeito
authors.forename: M L
authors.initials: ML
authors.lastname: Ortíz
authors.forename: F A
authors.initials: FA
authors.lastname: Lozza
authors.forename: P A
authors.initials: PA
authors.lastname: Fontana
authors.forename: E L
authors.initials: EL
authors.lastname: Portiansky
authors.forename: E J
authors.initials: EJ
authors.lastname: Gimeno
mesh_terms: D000818:Animals; D002117:Calcitriol / Q000633:toxicity*; D006001:Glycoconjugates / Q000737:chemistry / Q000378:metabolism*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009748:Nutrition Disorders / Q000662:veterinary*; D010939:Plant Poisoning / Q000662:veterinary*; D011817:Rabbits; D036061:Solanum glaucophyllum
chemical_list: D006001:Glycoconjugates; D002117:Calcitriol