title: Expression of the inhibitory CD200 receptor is associated with alternative macrophage activation.
abstract: Classical macrophage activation is inhibited by the CD200 receptor (CD200R). Here, we show that CD200R expression was specifically induced on human in vitro polarized macrophages of the alternatively activated M2a subtype, generated by incubation with IL-4 or IL-13. In mice, peritoneal M2 macrophages, elicited during infection with the parasites Taenia crassiceps or Trypanosoma brucei brucei, expressed increased CD200R levels compared to those derived from uninfected mice. However, in vitrostimulation of mouse peritoneal macrophages and T. crassiceps infection in IL-4-/- and IL-4R-/- mice showed that, in contrast to humans, induction of CD200R in mice was not IL-4 or IL-13 dependent. Our data identify CD200R as a suitable marker for alternatively activated macrophages in humans and corroborate observations of distinct species- and/or site-specific mechanisms regulating macrophage polarization in mouse and man.
authors.affiliation: Department of Neuroimmunology, Netherlands Institute for Neuroscience, Institute of the Royal Netherlands Academy for Arts and Sciences, Amsterdam, The Netherlands.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Koning
authors.forename: Marco
authors.initials: M
authors.lastname: van Eijk
authors.forename: Walter
authors.initials: W
authors.lastname: Pouwels
authors.forename: Michael S M
authors.initials: MS
authors.lastname: Brouwer
authors.forename: David
authors.initials: D
authors.lastname: Voehringer
authors.forename: Inge
authors.initials: I
authors.lastname: Huitinga
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoek
authors.forename: Geert
authors.initials: G
authors.lastname: Raes
authors.forename: Jörg
authors.initials: J
authors.lastname: Hamann
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D006801:Humans; D018793:Interleukin-13 / Q000276:immunology*; D015847:Interleukin-4 / Q000276:immunology*; D008262:Macrophage Activation / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000469:parasitology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013621:Taenia / Q000276:immunology; D013622:Taeniasis / Q000276:immunology / Q000469:parasitology; D014346:Trypanosoma brucei brucei / Q000276:immunology; D014353:Trypanosomiasis, African / Q000276:immunology / Q000469:parasitology
chemical_list: D015703:Antigens, CD; D018793:Interleukin-13; D015847:Interleukin-4; C042739:antigens, CD200