Drosophila macrophage preparation and screening.
Drosophila plasmatocytes, also known as macrophages, are part of the Drosophila innate immune system and also have roles during development. In late-stage embryos, it is possible to image macrophage migration in situ during development and when they converge at sites of wounding. This protocol describes the isolation of macrophages from third instar Drosophila larvae. The macrophages can be cultured for several hours, and fluorescently labeled macrophages can be screened using a fluorescence-imaging system.
Parton
Richard M
RM
Vallés
Ana Maria
AM
Dobbie
Ian M
IM
Davis
Ilan
I
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004330:Drosophila / Q000276:immunology*; D008264:Macrophages; D008403:Mass Screening; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling