title: Electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells.
abstract: It has been reported previously that electric pulses of sufficiently high voltage and short duration can permeabilize the membranes of various organelles inside living cells. In this article, we describe electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells. The cells were exposed to short, high-voltage electric pulses (from 1 to 20 pulses, 60 ns, 50 kV/cm, repetition frequency 1 kHz). We observed that 10 and 20 such pulses induced permeabilization of membranes of endocytotic vesicles, detected by release of lucifer yellow from the vesicles into the cytosol. Simultaneously, we detected uptake of propidium iodide through plasma membrane in the same cells. With higher number of pulses permeabilization of the membranes of endocytotic vesicles by pulses of given parameters is accompanied by permeabilization of plasma membrane. However, with lower number of pulses only permeabilization of the plasma membrane was detected.
authors.affiliation: Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, 1000, Ljubljana, Slovenia.
authors.forename: Tina Batista
authors.initials: TB
authors.lastname: Napotnik
authors.forename: Matej
authors.initials: M
authors.lastname: Rebersek
authors.forename: Tadej
authors.initials: T
authors.lastname: Kotnik
authors.forename: Eric
authors.initials: E
authors.lastname: Lebrasseur
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Cabodevila
authors.forename: Damijan
authors.initials: D
authors.lastname: Miklavcic
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000502:physiology; D018274:Electroporation / Q000379:methods*; D007546:Isoquinolines / Q000493:pharmacokinetics; D008546:Melanoma, Experimental / Q000378:metabolism*; D051379:Mice; D011419:Propidium / Q000493:pharmacokinetics; D022161:Transport Vesicles / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D007546:Isoquinolines; D011419:Propidium; C017475:lucifer yellow