title: Development of a luminescence-based biosensor for detection of methylmercury.
abstract: A recombinant whole-cell bacterial sensor for highly selective and sensitive detection of the bioavailable methylmercury in the environment was constructed. The biosensor carries luciferase gene, luxAB, from Vibrio harveyi as a reporter under the control of the mercury inducible regulatory part of mer-operon from Pseudomonas K-62 plasmid pMR26. In addition, a merB gene encoding organomercurial lyase which cleaves the C-Hg bond of methylmercury to give Hg(2+ )was coexpressed in the sensor. The resulting bacterial sensor responded specifically to methylmercury, and the lowest detectable concentration of methylmercury was 10 pM with 1 ml sample in the optimized assay conditions. This detection limit is enough to detect this compound in many contaminated and some pristine environmental samples.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagata
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Muraoka
authors.forename: Masako
authors.initials: M
authors.lastname: Kiyonoa
authors.forename: Hidemitsu
authors.initials: H
authors.lastname: Pan-Hou
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D008163:Luminescent Measurements; D008767:Methylmercury Compounds / Q000032:analysis*
chemical_list: D008767:Methylmercury Compounds