title: Reelin regulates postnatal neurogenesis and enhances spine hypertrophy and long-term potentiation.
abstract: Reelin, an extracellular protein essential for neural migration and lamination, is also expressed in the adult brain. To unravel the function of this protein in the adult forebrain, we generated transgenic mice that overexpress Reelin under the control of the CaMKIIalpha promoter. Overexpression of Reelin increased adult neurogenesis and impaired the migration and positioning of adult-generated neurons. In the hippocampus, the overexpression of Reelin resulted in an increase in synaptic contacts and hypertrophy of dendritic spines. Induction of long-term potentiation (LTP) in alert-behaving mice showed that Reelin overexpression evokes a dramatic increase in LTP responses. Hippocampal field EPSP during a classical conditioning paradigm was also increased in these mice. Our results indicate that Reelin levels in the adult brain regulate neurogenesis and migration, as well as the structural and functional properties of synapses. These observations suggest that Reelin controls developmental processes that remain active in the adult brain.
authors.affiliation: Developmental Neurobiology and Regeneration Laboratory, Institute for Research in Biomedicine, Barcelona, Department of Cell Biology, University of Barcelona, and Centro de Investigación en Red sobre Enfermedades Neurodegenerativas (CIBERNED-ISCIII), 08028 Barcelona, Spain. lluis.pujadas@irbbarcelona.org
authors.forename: Lluís
authors.initials: L
authors.lastname: Pujadas
authors.forename: Agnès
authors.initials: A
authors.lastname: Gruart
authors.forename: Carles
authors.initials: C
authors.lastname: Bosch
authors.forename: Lídia
authors.initials: L
authors.lastname: Delgado
authors.forename: Cátia M
authors.initials: CM
authors.lastname: Teixeira
authors.forename: Daniela
authors.initials: D
authors.lastname: Rossi
authors.forename: Luis
authors.initials: L
authors.lastname: de Lecea
authors.forename: Albert
authors.initials: A
authors.lastname: Martínez
authors.forename: José M
authors.initials: JM
authors.lastname: Delgado-García
authors.forename: Eduardo
authors.initials: E
authors.lastname: Soriano
mesh_terms: D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000502:physiology*; D002465:Cell Movement; D003214:Conditioning, Classical; D049229:Dendritic Spines / Q000648:ultrastructure*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000502:physiology*; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D017774:Long-Term Potentiation; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D055495:Neurogenesis; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure; D011401:Promoter Regions, Genetic; D016548:Prosencephalon / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000235:genetics / Q000502:physiology*; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases