title: HLA-DRB1*15 confers susceptibility to juvenile SLE but is not associated with disease presentation: an Egyptian Study.
abstract: The etiology of Systemic lupus erythematosus (SLE) seems to be multifactorial including environmental as well as genetic factors. Major histocompatibility complex (MHC) genes especially HLA-DRB1 and HLA-DQB1 are strongly implicated in susceptibility to SLE. Moreover ethnicity has been found to have a significant role in both disease susceptibility and disease expression. This study was carried out to determine HLA-DRB1 allele association with SLE susceptibility and disease presentation in Egyptian children with juvenile onset SLE. HLA-DRB1 allele typing was done using polymerase chain reaction-sequence-specific oligonucleotide probe for 65 juvenile Egyptian SLE patients and 150 healthy controls. p-values were corrected for the number of the alleles tested (Pc). HLA-DRB1*15 g allele was significantly increased in SLE children versus controls (OR = 4.76; 95% CI = 1.83-12.4; p = 0.001 and Pc = 0.012). No HLA-DRB1 allele was found to be statistically significant associated with musculoskeletal, cutaneous, hematologic, cardiac or neuropsychiatric manifestations in SLE patients (p > 0.05). Moreover no statistically significant association was found between HLA-DRB1 alleles and clinical presentation or histologic classes of lupus nephritis. The current work suggests that HLA-DRB1*15g allele may be a susceptibility allele in Egyptian children with SLE but is not related to clinical presentation of SLE.
authors.affiliation: Clinical Immunology Unit, Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura, Egypt. youssefmosaad@yahoo.com
authors.forename: Youssef M
authors.initials: YM
authors.lastname: Mosaad
authors.forename: Ayman
authors.initials: A
authors.lastname: Hammad
authors.forename: Hazem M
authors.initials: HM
authors.lastname: Youssef
authors.forename: Samir
authors.initials: S
authors.lastname: Elhanbly
mesh_terms: D000293:Adolescent; D017668:Age of Onset; D000483:Alleles; D002648:Child; D004252:DNA Mutational Analysis; D004534:Egypt; D005260:Female; D005500:Follow-Up Studies; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D008181:Lupus Nephritis; D008297:Male; D011110:Polymorphism, Genetic
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C507799:HLA-DRB1*15 antigen