Discovery of a gene conferring multiple-aminoglycoside resistance in Escherichia coli.
Bovine-origin Escherichia coli isolates were tested for resistance phenotypes using a disk diffusion assay and for resistance genotypes using a DNA microarray. An isolate with gentamicin and amikacin resistance but with no corresponding genes detected yielded a 1,056-bp DNA sequence with the closest homologues for its inferred protein sequence among a family of 16S rRNA methyltransferase enzymes. These enzymes confer high-level aminoglycoside resistance and have only recently been described in Gram-negative bacteria.
Davis
Margaret A
MA
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA. madavis@vetmed.wsu.edu
Baker
Katherine N K
KN
Orfe
Lisa H
LH
Shah
Devendra H
DH
Besser
Thomas E
TE
Call
Douglas R
DR
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D005798:Genes, Bacterial; D066298:In Vitro Techniques; D008780:Methyltransferases / Q000145:classification / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D012329:RNA, Bacterial / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000378:metabolism
D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase