[The influence of solcoseryl on population and cellular parameters of HeLa and RD cultures].
Changes of population and cellular parameters of HeLa and RD cultures after introducing of solcoseryl in culture medium were studied by methods of scanning cytophotometry and cytomorphometry. Monolayer density, proliferation activity, the number of dead cells in a monolayer, the number of nucleoli in nuclei and distribution of cells in the populations by this parameter, RNA and DNA masses in nuclei and nucleoli, total volumes and surface areas of the nuclei and nucleoli were determined. It has been shown that solcoseryl differently affects the cultures both on population and on cellular levels of their organization. The results of multi-parametric analysis of the influence of solseryl on the cultures allow considering it as a biologically active compound with the features typical for cell and cell population growth regulating factors.
Magakian
Iu A
IuA
Karalian
Z A
ZA
Karalova
E M
EM
Abroian
L O
LO
Akopian
L A
LA
Gasparian
M G
MG
Dzhagatspanian
N G
NG
Semerdzhian
Z B
ZB
Ter-Pogosian
Z R
ZR
D000183:Actihaemyl / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002466:Cell Nucleolus / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D003470:Culture Media; D003593:Cytoplasm / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D013997:Time Factors
D003470:Culture Media; D000183:Actihaemyl