title: Osmoregulated periplasmic glucan polymerization requires constant protein synthesis in Escherichia coli.
abstract: Osmoregulated periplasmic glucans are a family of oligosaccharides found in the periplasm of Gram negative bacteria. Mutants devoid of OPGs show strong reduction or absence of virulence on their hosts and display pleiotropic phenotype. Glucose is the sole constituent sugar and OPG level increases as the osmolarity of the medium decreases. OPG synthesis is regulated both at the transcriptional and at the enzymatic level. Data presented in this article indicate that in addition, OPG synthesis requires constant synthesis of protein indicating rapid turnover of one of the two proteins catalyzing glucose backbone of OPGs.
authors.affiliation: Unité de Glycobiologie Structurale et Fonctionnelle, UMR USTL-CNRS 8576 IFR 147, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France. jean-marie.lacroix@univ-lille1.fr
authors.forename: J-M
authors.initials: JM
authors.lastname: Lacroix
authors.forename: J-P
authors.initials: JP
authors.lastname: Bohin
mesh_terms: D002701:Chloramphenicol / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005936:Glucans / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D009154:Mutation; D009994:Osmolar Concentration; D019897:Periplasm / Q000378:metabolism*; D058105:Polymerization; D014882:Water-Electrolyte Balance
chemical_list: D029968:Escherichia coli Proteins; D005936:Glucans; D008565:Membrane Proteins; C492293:OpgG protein, E coli; D002701:Chloramphenicol; D019204:GTP-Binding Proteins; D005947:Glucose