title: Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3.
abstract: The intranuclear disposition of plasmid DNA is extremely important for transgene expression. Exogenous histones have been used as carriers of plasmid DNA in histone-mediated gene delivery. In this study, the effects of exogenous histone H3 complexed with plasmid DNA on transgene expression efficiency were examined. The plasmid-histone complexes in various ratios were transfected into HeLa cells by osmotic pressure. Histone H3 suppressed transgene expression in the nucleus in a dose-dependent manner. Our results suggest that the histone-mediated gene delivery is unlikely to be useful, from the viewpoint of the intranuclear disposition.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo, Japan. hirokam@pharm.hokudai.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamiya
authors.forename: Hitomi
authors.initials: H
authors.lastname: Goto
authors.forename: Genki
authors.initials: G
authors.lastname: Kanda
authors.forename: Yuma
authors.initials: Y
authors.lastname: Yamada
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
mesh_terms: D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006657:Histones / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008156:Luciferases / Q000235:genetics; D009997:Osmotic Pressure; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D019076:Transgenes
chemical_list: D004337:Drug Carriers; D006657:Histones; D004247:DNA; D008156:Luciferases