[Overexpression of adiponectin prevents hepatocyte steatosis].
OBJECTIVE
To investigate the effect of adiponectin on hepatocyte steatosis.


METHODS
L02 cells were transfected with pEGFP-N1-AdipoQ, a plasmid encoding pEGFP-adiponectin fusion protein, or pEGFP-N1. Lipid droplets in the hepatocytes were observed by oil red staining at 72 h. The contents of TG, FFA and glycerol in hepatocytes were measured.


RESULTS
Compared to cells transfected with pEGFP-N1-AdipoQ plasmid, much more lipid droplets were observed in cells transfected with pEGFP-N1 plasmid. TG, FFA and glycerol contents in L02 cells and L02/pEGFP-N1 cells were significantly higher than those in L02/pEGFP-N1-AdipoQ cells.


CONCLUSIONS
Overexpression of adiponectin prevent hepatocyte steatosis.
Zhou
Jing
J
Department of Clinincal, Chongqing Medical and Pharmaceutical College, Chongqing 400030, China.
Sun
Wen
W
Zhou
Zhou
Z
Wu
Ying
Y
Xiang
Ting-Xiu
TX
Jiang
Zheng
Z
Tao
Xiao-Hong
XH
Huang
Ai-Long
AL
Wang
Pi-Long
PL
D052242:Adiponectin / Q000235:genetics*; D002460:Cell Line; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005234:Fatty Liver / Q000378:metabolism; D005822:Genetic Vectors; D005990:Glycerol / Q000032:analysis; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014162:Transfection; D014280:Triglycerides / Q000032:analysis
D052242:Adiponectin; D005230:Fatty Acids, Nonesterified; D011993:Recombinant Fusion Proteins; D014280:Triglycerides; D005990:Glycerol