Inhibition of the osteoclast activity with the application of recombinant murine RANK protein.
OBJECTIVE
To investigate the effects of recombinant Murine RANK on the osteoclast activity.


METHODS
Osteoclast was observed with soluble RANK. Female Wistar rats were bilaterally ovariectomized, and intra-abdominally injected with 5 mg/Kg soluble RANK. The bone metabolism, bone density, and bone histomorphology were observed.


RESULTS
Compared with the control group, the quantity of TRAP-positive osteoclasts and bone resorption pit counting in the rest groups were significantly reduced. The bone density of the dosed groups was significantly increased and TRAP-stained osteoclasts in bone tissue sections were almost inhibited.


CONCLUSIONS
rh-Murine RANK was able to inhibit osteoclast differentiation and prevent ovariectomy-induced bone loss.
Tang
Peifu
P
Chinese PLA General Hospital, Department of Orthopedics, Beijing, PR China.
Zhang
Licheng
L
Xu
Meng
M
Zhang
Lihai
L
Wang
Yan
Y
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D001851:Bone Diseases, Metabolic / Q000188:drug therapy / Q000517:prevention & control; D001862:Bone Resorption / Q000097:blood / Q000188:drug therapy / Q000209:etiology / Q000503:physiopathology; D002118:Calcium / Q000097:blood; D002454:Cell Differentiation / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D051379:Mice; D015675:Osteocalcin / Q000097:blood; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010052:Ovariectomy / Q000009:adverse effects; D010758:Phosphorus / Q000097:blood; D051381:Rats; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D012995:Solubility
D053246:Receptor Activator of Nuclear Factor-kappa B; D011994:Recombinant Proteins; D015675:Osteocalcin; D010758:Phosphorus; D000469:Alkaline Phosphatase; D002118:Calcium