Difference of physiological characters in dark green islands and yellow leaf tissue of Cucumber mosaic virus (CMV)-infected Nicotiana tabacum leaves.
Dark green islands (DGIs) are a common symptom of plants systemically infected with the mosaic virus. DGIs are clusters of green leaf cells that are free of virus but surrounded by yellow leaf tissue that is full of virus particles. In Cucumber mosaic virus (CMV)-infected Nicotiana tabacum leaves, the respiration and photosynthesis capabilities of DGIs and yellow leaf tissues were measured. The results showed that the cyanide-resistant respiration was enhanced in yellow leaf tissue and the photosynthesis was declined, while in DGIs they were less affected. The activities of the oxygen-scavenging enzymes catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) in infected leaves were significantly higher than those in the healthy leaves, and the enzyme activities in DGIs were always lower than in the yellow leaf tissues. Reactive oxygen species (ROS) staining showed that the hydrogen peroxide content in yellow leaf tissues was apparently higher than that in DGIs, while the superoxide content was on the contrary. Formation of DGIs may be a strategy of the host plants resistance to the CMV infection.
Shang
Jing
J
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Science, Sichuan University, Chengdu 610064, Sichuan, China.
Xi
De-Hui
DH
Yuan
Shu
S
Xu
Fei
F
Xu
Mo-Yun
MY
Qi
Hai-Long
HL
Wang
Shao-Dong
SD
Huang
Qing-Rong
QR
Wen
Lin
L
Lin
Hong-Hui
HH
D002734:Chlorophyll / Q000378:metabolism; D017799:Cucumovirus / Q000472:pathogenicity*; D010788:Photosynthesis; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000502:physiology / Q000821:virology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012119:Respiration; D014026:Nicotiana / Q000378:metabolism / Q000821:virology*
D017382:Reactive Oxygen Species; D002734:Chlorophyll