Reactivation of immobilized acetylcholinesterase-tabun complex by pralidoxime, its isomers, and homologs.
Reactivation efficacy of three homologous and three isomeric series of pralidoxime-type reactivators with aldoxime group in position 2, 3 and 4 of the heterocycle was tested in reactivation of tabun-inhibited AChE. The experiments were performed with immobilized and stabilized porcine brain AChE. The enzyme activity was measured by Ellman method. Reactivation efficacy was determined by measurement of indicator fabric coloration intensity as a measure of AChE activity. Of the studied group of nine reactivators, isomers with the functional group in position 2 were the most effective. The highest value (30 %) for reactivation of inhibited AChE was found for 2PAE after treatment for 15 min at concentration 0.5 mg/cm(3). The efficacy of the isomers decreased in the order ortho > para > meta. No marked effect on the efficacy of the reactivators was observed on prolongation of the reactivation time. The reactivators efficacy decreased with decreasing concentration of their solutions.
Hoskovcová
Monika
M
University of Defence, Institute of NBC Defence, Víta Nejedlého, Vyskov, 682 03, Czech Republic. monika.hoskovcova@unob.cz
Halámek
Emil
E
Kobliha
Zbynek
Z
Tusarová
Ivana
I
D000110:Acetylcholinesterase / Q000187:drug effects*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D002801:Cholinesterase Reactivators / Q000494:pharmacology*; D007536:Isomerism; D010755:Organophosphates / Q000633:toxicity*; D011220:Pralidoxime Compounds / Q000737:chemistry / Q000494:pharmacology*; D013552:Swine
D002800:Cholinesterase Inhibitors; D002801:Cholinesterase Reactivators; D010755:Organophosphates; D011220:Pralidoxime Compounds; D000110:Acetylcholinesterase; C028797:pralidoxime; C009374:tabun