title: Differences in the structure and dynamics of the apo- and palmitate-ligated forms of Aedes aegypti sterol carrier protein 2 (AeSCP-2).
abstract: Sterol carrier protein-2 (SCP-2) is a nonspecific lipid-binding protein expressed ubiquitously in most organisms. Knockdown of SCP-2 expression in mosquitoes has been shown to result in high mortality in developing adults and significantly lowered fertility. Thus, it is of interest to determine the structure of mosquito SCP-2 and to identify its mechanism of lipid binding. We report here high quality three-dimensional solution structures of SCP-2 from Aedes aegypti determined by NMR spectroscopy in its ligand-free state (AeSCP-2) and in complex with palmitate. Both structures have a similar mixed alpha/beta fold consisting of a five-stranded beta-sheet and four alpha-helices arranged on one side of the beta-sheet. Ligand-free AeSCP-2 exhibited regions of structural heterogeneity, as evidenced by multiple two-dimensional (15)N heteronuclear single-quantum coherence peaks for certain amino acids; this heterogeneity disappeared upon complex formation with palmitate. The binding of palmitate to AeSCP-2 was found to decrease the backbone mobility of the protein but not to alter its secondary structure. Complex formation is accompanied by chemical shift differences and a loss of mobility for residues in the loop between helix alphaI and strand betaA. The structural differences between the alphaI and betaA of the mosquito and the vertebrate SCP-2s may explain the differential specificity (insect versus vertebrate) of chemical inhibitors of the mosquito SCP-2.
authors.affiliation: Department of Biochemistry, National Magnetic Resonance Facility at Madison, Wisconsin 53706, USA.
authors.forename: Kiran K
authors.initials: KK
authors.lastname: Singarapu
authors.forename: James T
authors.initials: JT
authors.lastname: Radek
authors.forename: Marco
authors.initials: M
authors.lastname: Tonelli
authors.forename: John L
authors.initials: JL
authors.lastname: Markley
authors.forename: Que
authors.initials: Q
authors.lastname: Lan
mesh_terms: D000330:Aedes / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D001051:Apoenzymes / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry; D005786:Gene Expression Regulation; D005821:Genetic Techniques; D008024:Ligands; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D019308:Palmitic Acid / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary
chemical_list: D001051:Apoenzymes; D002352:Carrier Proteins; D005227:Fatty Acids; D008024:Ligands; D008055:Lipids; C072087:sterol carrier proteins; D019308:Palmitic Acid