Development of a rapid, cost-effective TaqMan Real-Time PCR Assay for identification and differentiation of Coccidioides immitis and Coccidioides posadasii.
Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.
Sheff
Kelly W
KW
Translational Genomics Research Institute, Pathogen Genomics Division (TGen North), 3051 West Shamrell Boulevard, Flagstaff, Arizona, USA.
York
Emily R
ER
Driebe
Elizabeth M
EM
Barker
Bridget M
BM
Rounsley
Steven D
SD
Waddell
Victor G
VG
Beckstrom-Sternberg
Stephen M
SM
Beckstrom-Sternberg
James S
JS
Keim
Paul S
PS
Engelthaler
David M
DM
D003045:Coccidioides / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D003047:Coccidioidomycosis / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D013997:Time Factors