title: [ISSR analysis of genetic diversity of Coptis deltoidea].
abstract: OBJECTIVE
  To study the genetic diversity of Coptis deltoidea.
  
  
  METHODS
  The genetic diversity of 90 individuals from 8 populations was analyzed by ISSR.
  
  
  RESULTS
  Twelve primers were selected to produce highly reproducible ISSR bands. Among 128 amplified bands, 94 showed polymorphism, the percentage of polymorphic bands reached 73.44%. Nei's gene diversity index (H) was 0.1925, Shannon's information index (I) was 0.3028, Gst was 0.7212. The genetic distance coefficient and similarity were 0.0858-0.2314 and 0.8046-0.9425, respectively.
  
  
  CONCLUSIONS
  C. deltoidea held a high genetic diversity and the majority of genetic variation occurs among populations. By cluster analysis, the geographical distribution is very obvious. The ISSR marker can be used for the analysis of genetic diversity and genetic variation of C. deltoidea.
authors.affiliation: School of Life Sciences, Southwest University, Key Laboratory (Ministry of Education) of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and Resources Research for Three Gorges Reservoir Region, Chongqing 400715, China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.forename: Shijun
authors.initials: S
authors.lastname: Hu
authors.forename: Fenggang
authors.initials: F
authors.lastname: Yuan
authors.forename: Ruibo
authors.initials: R
authors.lastname: Wang
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
mesh_terms: D016000:Cluster Analysis; D039323:Coptis / Q000145:classification* / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D005828:Genetics, Population; D010802:Phylogeny; D011110:Polymorphism, Genetic / Q000235:genetics
chemical_list: D018744:DNA, Plant