The effect of nanotubular titanium surfaces on osteoblast differentiation.
The biological response of fetal rat calvarial cells on a TiO2 nanotubular surface (Ti-NT) was evaluated by cell viability assay, alkaline phosphatase (ALP) activity and reverse transcription polymerase chain reaction (RT-PCR) analysis. The cell viability assay showed no significant difference between the Ti-NT and smooth titanium surfaces (Ti-S). Ti-NT had better cellular responses with regard to the ALP activity and bone-associated markers, such as bone sialoprotein and osteocalcin mRNA than Ti-S. These results suggest that Ti-NT stimulate the differentiation into osteoblasts of fetal rat calvarial cells, potentially contributing to rapid osseointegration.
Kim
Jae-Hyung
JH
Dental Research Institute, School of Dentistry, 2nd Stage of Brain Korea 21 Project, Chonnam National University, Gwangju 500-757, Korea.
Cho
Kwan-Pyo
KP
Chung
Young-Suk
YS
Kim
Ok-Su
OS
Chung
Sung-Soo
SS
Lee
Kyung-Ku
KK
Lee
Doh-Jae
DJ
Lee
Kwang-Min
KM
Kim
Young-Joon
YJ
D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D010316:Particle Size; D051381:Rats; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D046911:Macromolecular Substances; C009495:titanium dioxide; D014025:Titanium