title: 3D nanoscale imaging of the yeast, Schizosaccharomyces pombe, by full-field transmission X-ray microscopy at 5.4 keV.
abstract: Three-dimensional (3D) nanoscale structures of the fission yeast, Schizosaccharomyces pombe, can be obtained by full-field transmission hard X-ray microscopy with 30 nm resolution using synchrotron radiation sources. Sample preparation is relatively simple and the samples are portable across various imaging environments, allowing for high-throughput sample screening. The yeast cells were fixed and double-stained with Reynold's lead citrate and uranyl acetate. We performed both absorption contrast and Zernike phase contrast imaging on these cells in order to test this method. The membranes, nucleus, and subcellular organelles of the cells were clearly visualized using absorption contrast mode. The X-ray images of the cells could be used to study the spatial distributions of the organelles in the cells. These results show unique structural information, demonstrating that hard X-ray microscopy is a complementary method for imaging and analyzing biological samples.
authors.affiliation: National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui 230029, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Yunhao
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
authors.forename: Joy C
authors.initials: JC
authors.lastname: Andrews
authors.forename: Piero
authors.initials: P
authors.lastname: Pianetta
authors.forename: Yong
authors.initials: Y
authors.lastname: Guan
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Wu
authors.forename: Yangchao
authors.initials: Y
authors.lastname: Tian
mesh_terms: D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D015388:Organelles / Q000648:ultrastructure; D012568:Schizosaccharomyces / Q000166:cytology*; D013194:Staining and Labeling; D017356:Synchrotrons; D014965:X-Rays