Effects of mating on progesterone receptor isoforms in rat hypothalamus.
In rodents, the display of sexual behavior during proestrus-estrus transition depends on the effect of estradiol and progesterone. Progesterone exerts its effects through intracellular receptor (PR) of which two isoforms (PR-A and PR-B) are found, with different regulation and function. In this study the effects of mating on the expression pattern of PR isoforms in the hypothalamus were investigated during proestrus-estrus transition by using western blot. PR-B isoform content significantly diminished during proestrus-estrus transition both in mated and nonmated female rats. In contrast, PR-A isoform content significantly increases during this period in nonmated rats, whereas it does not change in mated animals. These data show that PR isoforms are differentially expressed throughout proestrus-estrus transition and that mating modifies PR isoforms expression in the hypothalamus of the rat.
Mendoza-Garcés
Luciano
L
Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, México, D.F., Mexico.
Camacho-Arroyo
Ignacio
I
Cerbón
Marco A
MA
D000818:Animals; D015153:Blotting, Western; D004971:Estrus / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D011359:Proestrus / Q000378:metabolism; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism*; D012726:Sexual Behavior, Animal / Q000502:physiology*
D020033:Protein Isoforms; D011980:Receptors, Progesterone