Extraction of polysaccharide from Ganoderma lucidum and its immune enhancement activity.
In the present study, in order to maximize the yield of polysaccharides from Ganoderma lucidum, response surface methodology was employed to optimize the ultrasonic/microwave-assisted extraction (UMAE) conditions. The results indicated that the optimal extraction conditions were ultrasonic power of 50W, microwave power of 284W, extraction time of 701s and water/solid ratio of 11.6:1, respectively. Using UMAE, the yield of polysaccharides was 115.56% above that of classical hot water extraction (HWE) and increased by 27.7% as against ultrasound-assisted extraction (UAE), which confirmed the great potential application of UMAE technology in the extraction of polysaccharides. The immunological assays results demonstrated that polysaccharides of G. lucidum extracted by ultrasonic/microwave (UMP) could improve the weight of immune organ of immunocompromised mice, restore delayed-type hypersensitivity (DTH) reaction to DFNB, improve hemolysis antibody level and natural killer cell activity at high-dose. However, UMP had no noticeable effects on phagocytosis of monocyte at the tested dosage range.
Huang
Sheng-quan
SQ
South China University of Technology, Guangdong Province, Guangzhou, China.
Ning
Zheng-xiang
ZX
D000818:Animals; D002244:Carbon / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D006460:Hemolysin Proteins / Q000097:blood; D006358:Hot Temperature; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007155:Immunologic Factors / Q000276:immunology* / Q000302:isolation & purification*; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D051379:Mice; D008872:Microwaves; D011134:Polysaccharides / Q000276:immunology* / Q000302:isolation & purification*; D020940:Reishi / Q000737:chemistry*; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014465:Ultrasonics; D014867:Water / Q000737:chemistry
D006460:Hemolysin Proteins; D007155:Immunologic Factors; D011134:Polysaccharides; D014867:Water; D002244:Carbon