Surface functionalization of titanium with chitosan/gelatin via electrophoretic deposition: characterization and cell behavior.
The electrophoretic deposition (EPD) is a versatile and cost-effective technique for fabricating advanced coatings. In this study, chitosan/gelatin (CS/G) coatings were prepared on titanium substrates via EPD. The prepared coatings were characterized using fluorescence microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and shear bond strength testing. It was found that CS/G coatings had a similar macroporous structure. The gelatin content in the CS/G coatings gradually increased with the increase of the gelatin in the blend solutions. The shear bond strength of the CS/G coatings also increased with the increasing gelatin content. In vitro biological tests demonstrated that human MG63 osteoblast-like cells achieved better affinity on the coatings with higher gelatin content. Therefore, it was concluded that EPD was an effective and efficient technique to prepare CS/G coatings on the titanium surface and that CS/G coatings with higher gelatin content were promising candidates for further loading of functional agents.
Jiang
Tao
T
Key Laboratory for Oral Biomedical Engineering, Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, People's Republic of China.
Zhang
Zhen
Z
Zhou
Yi
Y
Liu
Yi
Y
Wang
Zhejun
Z
Tong
Hua
H
Shen
Xinyu
X
Wang
Yining
Y
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
D005780:Gelatin; D048271:Chitosan; D014025:Titanium