title: Systematic analysis of human protein complexes identifies chromosome segregation proteins.
abstract: Chromosome segregation and cell division are essential, highly ordered processes that depend on numerous protein complexes. Results from recent RNA interference screens indicate that the identity and composition of these protein complexes is incompletely understood. Using gene tagging on bacterial artificial chromosomes, protein localization, and tandem-affinity purification-mass spectrometry, the MitoCheck consortium has analyzed about 100 human protein complexes, many of which had not or had only incompletely been characterized. This work has led to the discovery of previously unknown, evolutionarily conserved subunits of the anaphase-promoting complex and the gamma-tubulin ring complex--large complexes that are essential for spindle assembly and chromosome segregation. The approaches we describe here are generally applicable to high-throughput follow-up analyses of phenotypic screens in mammalian cells.
authors.affiliation: Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
authors.forename: James R A
authors.initials: JR
authors.lastname: Hutchins
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Toyoda
authors.forename: Björn
authors.initials: B
authors.lastname: Hegemann
authors.forename: Ina
authors.initials: I
authors.lastname: Poser
authors.forename: Jean-Karim
authors.initials: JK
authors.lastname: Hériché
authors.forename: Martina M
authors.initials: MM
authors.lastname: Sykora
authors.forename: Martina
authors.initials: M
authors.lastname: Augsburg
authors.forename: Otto
authors.initials: O
authors.lastname: Hudecz
authors.forename: Bettina A
authors.initials: BA
authors.lastname: Buschhorn
authors.forename: Jutta
authors.initials: J
authors.lastname: Bulkescher
authors.forename: Christian
authors.initials: C
authors.lastname: Conrad
authors.forename: David
authors.initials: D
authors.lastname: Comartin
authors.forename: Alexander
authors.initials: A
authors.lastname: Schleiffer
authors.forename: Mihail
authors.initials: M
authors.lastname: Sarov
authors.forename: Andrei
authors.initials: A
authors.lastname: Pozniakovsky
authors.forename: Mikolaj Michal
authors.initials: MM
authors.lastname: Slabicki
authors.forename: Siegfried
authors.initials: S
authors.lastname: Schloissnig
authors.forename: Ines
authors.initials: I
authors.lastname: Steinmacher
authors.forename: Marit
authors.initials: M
authors.lastname: Leuschner
authors.forename: Andrea
authors.initials: A
authors.lastname: Ssykor
authors.forename: Steffen
authors.initials: S
authors.lastname: Lawo
authors.forename: Laurence
authors.initials: L
authors.lastname: Pelletier
authors.forename: Holger
authors.initials: H
authors.lastname: Stark
authors.forename: Kim
authors.initials: K
authors.lastname: Nasmyth
authors.forename: Jan
authors.initials: J
authors.lastname: Ellenberg
authors.forename: Richard
authors.initials: R
authors.lastname: Durbin
authors.forename: Frank
authors.initials: F
authors.lastname: Buchholz
authors.forename: Karl
authors.initials: K
authors.lastname: Mechtler
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Hyman
authors.forename: Jan-Michael
authors.initials: JM
authors.lastname: Peters
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome; D018385:Centrosome / Q000378:metabolism; D020090:Chromosome Segregation; D022202:Chromosomes, Artificial, Bacterial; D030541:Databases, Genetic; D023281:Genomics; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D046912:Multiprotein Complexes / Q000378:metabolism*; D016366:Open Reading Frames; D011485:Protein Binding; D025941:Protein Interaction Mapping; D021122:Protein Subunits / Q000378:metabolism; D034622:RNA Interference; D008941:Spindle Apparatus / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*; D043743:Ubiquitin-Protein Ligase Complexes / Q000378:metabolism*
chemical_list: D046912:Multiprotein Complexes; D021122:Protein Subunits; D014404:Tubulin; D049452:Green Fluorescent Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome