title: Xenografting restores spermatogenesis to cryptorchid testicular tissue but does not rescue the phenotype of idiopathic testicular degeneration in the horse (Equus caballus).
abstract: Spermatogenesis from many mammalian species occurs in fragments of normal testis tissue xenografted to mice. Here we apply xenografting to the study of testicular pathology. Using the horse model, we investigated whether exposure to a permissive extratesticular environment in the mouse host would rescue spermatogenesis in cryptorchid testicular tissue or in tissue affected by idiopathic testicular degeneration (ITD). In cryptorchid tissue, where the extratesticular environment is abnormal, xenografting induced spermatogenesis up to meiosis in a subpopulation of seminiferous tubules. Thus, spermatogonia survive and partially retain their potential to differentiate in cryptorchid horse testes. In contrast, the primary defect in equine ITD is hypothesised to be tissue autologous. In support of this, xenografting did not restore spermatogenesis to tissue affected by ITD, thus confirming that the testis itself is primarily diseased. This outcome was not affected by supplementation of exogenous gonadotropins to the mouse host or by reconstitution of a normal reproductive regulatory axis supplied by functional porcine testicular xenografts. These studies demonstrate the usefulness of xenografting for the study of testicular pathology.
authors.affiliation: Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Studies, New Bolton Center, University of Pennsylvania, School of Veterinary Medicine, 382 West Street Road, Kennett Square, PA 19348, USA.
authors.forename: Regina M
authors.initials: RM
authors.lastname: Turner
authors.forename: Rahul
authors.initials: R
authors.lastname: Rathi
authors.forename: Ali
authors.initials: A
authors.lastname: Honaramooz
authors.forename: Wenxian
authors.initials: W
authors.lastname: Zeng
authors.forename: Ina
authors.initials: I
authors.lastname: Dobrinski
mesh_terms: D000367:Age Factors; D000818:Animals; D003456:Cryptorchidism / Q000473:pathology / Q000601:surgery / Q000662:veterinary*; D004195:Disease Models, Animal; D006651:Histocytochemistry; D006734:Horse Diseases / Q000473:pathology / Q000628:therapy*; D006736:Horses; D008297:Male; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000637:transplantation*; D014183:Transplantation, Heterologous / Q000379:methods / Q000662:veterinary*