title: Biscuit melanoidins of different molecular masses protect human HepG2 cells against oxidative stress.
abstract: Soluble melanoidins from biscuits were enzymatically solubilized and isolated by sequential ultrafiltration and separated by molecular mass in three different fractions, below 3 kDa, between 3 and 10 kDa, and over 10 kDa; the latter was subsequently digested by simulating gastric plus pancreatic digestive conditions. The four fractions were investigated for their protective effect against an oxidative challenge in HepG2 cells. Pretreatment of cells for 20 h with 0.5-10 microg/mL of any of the four fractions prevented the increased cell damage evoked by the challenge but, except for the intermediate size fraction, did not suppress the increased reactive oxygen species. Antioxidant defenses were rapidly restored after the challenge, and the increase of the oxidative stress biomarker malondialdehyde was prevented by the pretreatment with all but the undigested high molecular mass fraction. The results show that treatment of HepG2 cells with concentrations of biscuit melanoidins within the expected physiological range confers on the cells a significant protection against an oxidative challenge.
authors.affiliation: Consejo Superior de Investigaciones Cientificas, Instituto del Frio - ICTAN, Jose Antonio Novais 10, Madrid, Spain.
authors.forename: María Angeles
authors.initials: MA
authors.lastname: Martín
authors.forename: Sonia
authors.initials: S
authors.lastname: Ramos
authors.forename: Raquel
authors.initials: R
authors.lastname: Mateos
authors.forename: José Angel
authors.initials: JA
authors.lastname: Rufián-Henares
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Morales
authors.forename: Laura
authors.initials: L
authors.lastname: Bravo
authors.forename: Luis
authors.initials: L
authors.lastname: Goya
mesh_terms: D001939:Bread / Q000032:analysis*; D056945:Hep G2 Cells; D006801:Humans; D008970:Molecular Weight; D018384:Oxidative Stress / Q000187:drug effects*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D011108:Polymers; D020011:Protective Agents; C011908:melanoidin polymers