title: Promoter diversity in multigene transformation.
abstract: Multigene transformation (MGT) is becoming routine in plant biotechnology as researchers seek to generate more complex and ambitious phenotypes in transgenic plants. Every nuclear transgene requires its own promoter, so when coordinated expression is required, the introduction of multiple genes leads inevitably to two opposing strategies: different promoters may be used for each transgene, or the same promoter may be used over and over again. In the former case, there may be a shortage of different promoters with matching activities, but repetitious promoter use may in some cases have a negative impact on transgene stability and expression. Using illustrative case studies, we discuss promoter deployment strategies in transgenic plants that increase the likelihood of successful and stable multiple transgene expression.
authors.affiliation: Departament de Producció Vegetal i Ciència Forestal, ETSEA, Universitat de Lleida, Av. Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors.forename: Ariadna
authors.initials: A
authors.lastname: Peremarti
authors.forename: Richard M
authors.initials: RM
authors.lastname: Twyman
authors.forename: Sonia
authors.initials: S
authors.lastname: Gómez-Galera
authors.forename: Shaista
authors.initials: S
authors.lastname: Naqvi
authors.forename: Gemma
authors.initials: G
authors.lastname: Farré
authors.forename: Maite
authors.initials: M
authors.lastname: Sabalza
authors.forename: Bruna
authors.initials: B
authors.lastname: Miralpeix
authors.forename: Svetlana
authors.initials: S
authors.lastname: Dashevskaya
authors.forename: Dawei
authors.initials: D
authors.lastname: Yuan
authors.forename: Koreen
authors.initials: K
authors.lastname: Ramessar
authors.forename: Paul
authors.initials: P
authors.lastname: Christou
authors.forename: Changfu
authors.initials: C
authors.lastname: Zhu
authors.forename: Ludovic
authors.initials: L
authors.lastname: Bassie
authors.forename: Teresa
authors.initials: T
authors.lastname: Capell
mesh_terms: D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013997:Time Factors; D014170:Transformation, Genetic / Q000235:genetics*