title: Clinical and serological evaluation of Leptospirosis in Puducherry, India.
abstract: BACKGROUND
  Leptospirosis is a zoonotic disease with a worldwide distribution. There is a paucity of available data about prevalence of this disease in Pondicherry. Our aim was to investigate the seropositivity rate of leptospirosis in suspected cases and also to identify the predominant serogroups present by performing Microscopic Agglutination Test (MAT). The other aim of this study was to compare the results of a commercially available IgM ELISA with that of MAT.
  
  
  METHODS
  A total of 110 blood samples from patients suspected of leptospirosis were sent for diagnosis. These samples were subjected to IgM ELISA and the microscopic agglutination test (MAT). MAT was done using a panel of 12 Leptospira serovars.
  
  
  RESULTS
  MAT analysis of the 110 samples showed 40 (36%) to be positive. Antibodies were predominantly seen against serogroup Leptospira Icterohemorrhagiae (27%), followed by Pomona (17%), and Pyrogenes (12%). IgM ELISA done on these samples showed a positivity of 37% compared to MAT.
  
  
  CONCLUSIONS
  This study reveals that the MAT test can be standardized in a diagnostic laboratory and used in conjunction with an IgM ELISA.
authors.affiliation: Department of Microbiology, Jawaharlal Institute of Post graduate Medical Education and Research, Puducherry, India. smithsmile14@rediffmail.com
authors.forename: Smita Benjamin
authors.initials: SB
authors.lastname: Shekatkar
authors.forename: Belgode Narasimha
authors.initials: BN
authors.lastname: Harish
authors.forename: Godfred Antony
authors.initials: GA
authors.lastname: Menezes
authors.forename: Subhash Chandra
authors.initials: SC
authors.lastname: Parija
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000372:Agglutination Tests / Q000379:methods; D000907:Antibodies, Bacterial / Q000097:blood; D019937:Diagnostic Techniques and Procedures; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007194:India / Q000453:epidemiology; D007919:Leptospira / Q000276:immunology; D007922:Leptospirosis / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies
chemical_list: D000907:Antibodies, Bacterial; D007075:Immunoglobulin M