Alterations of telomere length in human brain tumors.
Telomeres at the ends of human chromosomes consist of tandem hexametric (TTAGGG)n repeats, which protect them from degradation. At each cycle of cell division, most normal somatic cells lose approximately 50-100 bp of the terminal telomeric repeat DNA. Precise prediction of growth and estimation of the malignant potential of brain tumors require additional markers. DNA extraction was performed from the 51 frozen tissues, and a non-radioactive chemiluminescent assay was used for Southern blotting. One sample t-test shows highly significant difference in telomere length in meningioma and astrocytoma with normal range. According to our results, higher grades of meningioma and astrocytoma tumors show more heterogeneity in telomere length, and also it seems shortening process of telomeres is an early event in brain tumors.
Kheirollahi
Majid
M
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, and Shariati Hospital, Tehran, Iran. mkheirollahi@tums.ac.ir
Mehrazin
Masoud
M
Kamalian
Naser
N
Mehdipour
Parvin
P
D001254:Astrocytoma / Q000378:metabolism / Q000473:pathology; D015139:Blotting, Southern; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D004806:Ependymoma / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008579:Meningioma / Q000378:metabolism / Q000473:pathology; D016615:Telomere / Q000378:metabolism* / Q000473:pathology