title: Hydrophobic fractions from Strongyloides venezuelensis for use in the human immunodiagnosis of strongyloidiasis.
abstract: The objective of the present research was to evaluate detergent and aqueous phases of total saline (TS) and alkaline extracts of Strongyloides venezuelensis for human strongyloidiasis immunodiagnosis. Total extracts and detergent and aqueous antigenic fractions were separated using Triton X-114 and were examined by enzyme-linked immunosorbent assay (ELISA) and immunoblotting (IB) tests to detect immunoglobulin G (IgG). Serum samples were obtained from 120 individuals: 40 strongyloidiasis patients (group I), 40 patients with other parasitic diseases (group II), and 40 apparently healthy individuals (group III). Each extract provided a different profile of antigenic components as recognized by IgG in IB. The detergent fraction of the TS extract demonstrated the highest sensitivity and specificity for ELISA and IB. The results indicated that the detergent saline fraction, purified from S. venezuelensis, furnished the most valid results for the strongyloidiasis immunodiagnosis and could be employed as an alternative antigen and as a useful source of specific polypeptides.
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, 38400-902, Uberlândia, Minas Gerais, Brazil.
authors.forename: Nágilla Daliane
authors.initials: ND
authors.lastname: Feliciano
authors.forename: Henrique Tomaz
authors.initials: HT
authors.lastname: Gonzaga
authors.forename: Maria do Rosário Fátima
authors.initials: Mdo R
authors.lastname: Gonçalves-Pires
authors.forename: Ana Lúcia Ribeiro
authors.initials: AL
authors.lastname: Gonçalves
authors.forename: Rosangela Maria
authors.initials: RM
authors.lastname: Rodrigues
authors.forename: Marlene Tiduko
authors.initials: MT
authors.lastname: Ueta
authors.forename: Julia Maria
authors.initials: JM
authors.lastname: Costa-Cruz
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D007159:Immunologic Tests / Q000379:methods; D010274:Parasitology / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013321:Strongyloides / Q000737:chemistry* / Q000276:immunology*; D013322:Strongyloidiasis / Q000175:diagnosis*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G