Inactivation of MS2 coliphage in Fenton and Fenton-like systems: role of transition metals, hydrogen peroxide and sunlight.
The inactivation of coliphage MS2 by iron- and copper-catalyzed Fenton systems was studied to assess the importance of this process for virus inactivation in natural systems and during water treatment by advanced oxidation processes. The influence of H(2)O(2) (3-50 microM) and metal (1-10 microM) concentrations, HO(*) production, and sunlight on inactivation was investigated. Inactivation was first order with respect to H(2)O(2), but the dependence on the metal concentration was more complex. In the Cu/H(2)O(2) system, the inactivation rate constant k(obs) increased with added Cu up to 2.5 microM, and then leveled off. This was consistent with Cu saturation of the solution, indicating that only soluble Cu contributed to inactivation. In contrast, inactivation in the Fe/H(2)O(2) system was governed by colloidal iron. Irradiation by sunlight only affected the Fe/H(2)O(2) system, leading to a 5.5-fold increase in k(obs) (up to 3.1 min(-1)). HO(*) production, measured by electron spin resonance, could not account for the observed inactivation in the Fe/H(2)O(2) system. Other oxidants, such as ferryl species, must therefore play a role. Experiments using bulk oxidant scavengers revealed that inactivation occurred by a caged mechanism involving oxidant production by metals located in close proximity to the virus. Overall, our results show that the Fenton/photo-Fenton process may serve as an efficient technology for virus disinfection.
Nieto-Juarez
Jessica I
JI
Ecole Polytechnique Federale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
Pierzchła
Katarzyna
K
Sienkiewicz
Andrzej
A
Kohn
Tamar
T
D000327:Adsorption; D002621:Chemistry / Q000379:methods; D003300:Copper / Q000737:chemistry; D004202:Disinfectants / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical; D007501:Iron / Q000737:chemistry; D007700:Kinetics; D017909:Levivirus / Q000378:metabolism*; D008027:Light; D008670:Metals / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D013472:Sunlight; D014873:Water Pollutants
D004202:Disinfectants; D008670:Metals; D014873:Water Pollutants; D017665:Hydroxyl Radical; D003300:Copper; D006861:Hydrogen Peroxide; D007501:Iron; D010100:Oxygen