title: Establishment of a novel ghrelin-producing cell line.
abstract: To establish a tool to study ghrelin production and secretion in vitro, we developed a novel ghrelin-producing cell line, MGN3-1 (mouse ghrelinoma 3-1) cells from a gastric ghrelin-producing cell tumor derived from ghrelin-promoter Simian virus 40-T-antigen transgenic mice. MGN3-1 cells preserve three essential characteristics required for the in vitro tool for ghrelin research. First, MGN3-1 cells produce a substantial amount of ghrelin at levels approximately 5000 times higher than that observed in TT cells. Second, MGN3-1 cell expressed two key enzymes for acyl modification and maturation of ghrelin, namely ghrelin O-acyltransferase for acylation and prohormone convertase 1/3 for maturation and the physiological acyl modification and maturation of ghrelin were confirmed. Third, MGN3-1 cells retain physiological regulation of ghrelin secretion, at least in regard to the suppression by somatostatin and insulin, which is well established in in vivo studies. Thus, MGN3-1 cells are the first cell line derived from a gastric ghrelin-producing cell preserving secretion of substantial amounts of ghrelin under physiological regulation. This cell line will be a useful tool for both studying the production and secretion of ghrelin and screening of ghrelin-modulating drugs.
authors.affiliation: 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan. hiwaku@kuhp.kyoto-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iwakura
authors.forename: Yushu
authors.initials: Y
authors.lastname: Li
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ariyasu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hosoda
authors.forename: Naotetsu
authors.initials: N
authors.lastname: Kanamoto
authors.forename: Mika
authors.initials: M
authors.lastname: Bando
authors.forename: Go
authors.initials: G
authors.lastname: Yamada
authors.forename: Kiminori
authors.initials: K
authors.lastname: Hosoda
authors.forename: Kazuwa
authors.initials: K
authors.lastname: Nakao
authors.forename: Kenji
authors.initials: K
authors.lastname: Kangawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Akamizu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D054439:Ghrelin / Q000097:blood* / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D046529:Microscopy, Electron, Transmission; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D054439:Ghrelin