Maleopimaric acid anhydride-bonded silica monolith as chiral stationary phase for separations of phenylthiocarbamyl amino acids by CEC.
A new type of chiral silica monolith was successfully developed for the enantioseparation of phenylthiocarbamyl amino acids (PTC-(D/L)-AAs) by CEC. The bare monolithic silica column was prepared by a sol-gel process and has been modified chemically with aminopropyltrimethoxysilane, followed by immobilization of a chiral selector maleopimaric acid anhydride. Eight pairs of tested amino acids enantiomers including PTC-(D/L)-arginine, PTC-(D/L)-valine, PTC-(D/L)-leucine, PTC-(D/L)-4-methylphenylalanine, PTC-(D/L)-phenylalanine, PTC-(D/L)-beta-phenylalanine, PTC-(D/L)-tryptophan and PTC-(D/L)-kynurenine were well resolved with high column efficiencies. The influences of organic modifier in mobile phase on apparent retention factor, enantioselectivity factor and resolution were also investigated.
Ye
Fanggui
F
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin, PR China. fangguiye@163.com
Wang
Hengshan
H
Huang
Baojun
B
Zhao
Shulin
S
D000097:Acetonitriles / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D053801:Capillary Electrochromatography / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D044367:Phase Transition; D048448:Phenylcarbamates / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism; D013859:Thiocarbamates / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry*
D000097:Acetonitriles; D000596:Amino Acids; D048448:Phenylcarbamates; D013859:Thiocarbamates; D014315:Triterpenes; C027815:maleopimaric acid; D012822:Silicon Dioxide; C032159:acetonitrile