title: Visceral fat decreased by long-term interdisciplinary lifestyle therapy correlated positively with interleukin-6 and tumor necrosis factor-α and negatively with adiponectin levels in obese adolescents.
abstract: The purpose of this study was to assess the level of cytokine expression in correlation with visceral and subcutaneous fat in obese adolescents admitted to long-term interdisciplinary weight loss therapy. The study was a longitudinal clinical intervention of interdisciplinary therapy. Adolescents (18, aged 15-19 years) with body mass indexes greater than the 95th percentile were admitted and evaluated at baseline and again after 1 year of interdisciplinary therapy. Visceral and subcutaneous fat was analyzed by ultrasonography. Blood samples were collected to analyze tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), and adiponectin concentrations that were measured by enzyme-linked immunosorbent assay. The most important finding in the present investigation is that the long-term interdisciplinary lifestyle therapy decreased visceral fat. Positive correlations between IL-6 levels and visceral fat (r = 0.42, P < .02) and TNF-α levels and visceral fat (r = 0.40, P < .05) were observed. Negative correlations between TNF-α levels and subcutaneous fat (r = -0.46, P < .01) and adiponectin levels and subcutaneous fat (r = -0.43, P < .03) were also observed. In addition, we found a positive correlation between TNF-α levels and the visceral to subcutaneous fat ratio (r = 0.42, P < .02) and a negative correlation between adiponectin level and the visceral to subcutaneous fat ratio (r = -0.69, P < .001). Despite the limitation of sample size, our results indicate that the observed massive weight loss (mainly visceral fat) was highly correlated with a decreased inflammatory state, suggesting that the interdisciplinary therapy was effective in decreasing inflammatory markers.
authors.affiliation: Postgraduate Program of Nutrition, Federal University of São Paulo-UNIFESP, São Paulo/SP 04020-060, Brazil. fabioslira@gmail.com
authors.forename: Fábio Santos
authors.initials: FS
authors.lastname: Lira
authors.forename: Jose Cesar
authors.initials: JC
authors.lastname: Rosa
authors.forename: Ronaldo Vagner
authors.initials: RV
authors.lastname: Dos Santos
authors.forename: Daniel Paulino
authors.initials: DP
authors.lastname: Venancio
authors.forename: June
authors.initials: J
authors.lastname: Carnier
authors.forename: Priscila de Lima
authors.initials: Pde L
authors.lastname: Sanches
authors.forename: Claudia Maria Oller
authors.initials: CM
authors.lastname: do Nascimento
authors.forename: Aline
authors.initials: A
authors.lastname: de Piano
authors.forename: Lian
authors.initials: L
authors.lastname: Tock
authors.forename: Sergio
authors.initials: S
authors.lastname: Tufik
authors.forename: Marco Túlio
authors.initials: MT
authors.lastname: de Mello
authors.forename: Ana R
authors.initials: AR
authors.lastname: Dâmaso
authors.forename: Lila Missae
authors.initials: LM
authors.lastname: Oyama
mesh_terms: D052242:Adiponectin / Q000097:blood; D000293:Adolescent; D001521:Behavior Therapy / Q000379:methods*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D050152:Intra-Abdominal Fat / Q000000981:diagnostic imaging / Q000378:metabolism*; D008137:Longitudinal Studies; D008297:Male; D009765:Obesity / Q000097:blood / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000628:therapy*; D018709:Statistics, Nonparametric; D050151:Subcutaneous Fat / Q000000981:diagnostic imaging / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014463:Ultrasonography; D015431:Weight Loss; D055815:Young Adult
chemical_list: D052242:Adiponectin; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10