[Determination of streptomycin in honey determined by hydrophilic interaction chromatography combined with tandem mass spectrometry].
OBJECTIVE
To establish a method for the determination of streptomycin in honey by determined hydrophilic interaction chromatography combined with tandem mass spectrometry (HILIC-MS/MS).


METHODS
A hilic column was used, and the mobile phase was consisted of acetonitrile (5 mmol/L ammonium acetate and 0.1% formic acid, 60:40). Samples were extracted by the cation-exchange SPE cartridge. The extraction samples were then identified and quantitated by HILIC-ESI-MS/MS.


RESULTS
Linear calibration curves were obtained at the concentration ranges from 10. 5 microg/kg to 105 microg/kg (the correlation coefficients were above 0.99). The limit of detection was 1.05 microg/kg. The average recoveries for streptomycin spiked in honey ranged from 71.3 to 82.5% and their relative standard were between 3.4% and 8.2%.


CONCLUSIONS
The method for determination of streptomycin in honey could be simple, sensitive and accurate.
Chen
Lingyun
L
Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. yun-niu@163.com
Zhou
Zhifeng
Z
Ma
Ande
A
D002845:Chromatography / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D057927:Hydrophobic and Hydrophilic Interactions; D013307:Streptomycin / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D013307:Streptomycin