Cholesterol sulfate imaging in human prostate cancer tissue by desorption electrospray ionization mass spectrometry.
Development of methods for rapid distinction between cancerous and non-neoplastic tissues is an important goal in disease diagnosis. To this end, desorption electrospray ionization mass spectrometry (DESI-MS) imaging was applied to analyze the lipid profiles of thin tissue sections of 68 samples of human prostate cancer and normal tissue. The disease state of the tissue sections was determined by independent histopathological examination. Cholesterol sulfate was identified as a differentiating compound, found almost exclusively in cancerous tissues including tissue containing precancerous lesions. The presence of cholesterol sulfate in prostate tissues might serve as a tool for prostate cancer diagnosis although confirmation through larger and more diverse cohorts and correlations with clinical outcome data is needed.
Eberlin
Livia S
LS
Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University, West Lafayette, Indiana 47907, USA.
Dill
Allison L
AL
Costa
Anthony B
AB
Ifa
Demian R
DR
Cheng
Liang
L
Masterson
Timothy
T
Koch
Michael
M
Ratliff
Timothy L
TL
Cooks
R Graham
RG
D002788:Cholesterol Esters / Q000032:analysis / Q000737:chemistry*; D003952:Diagnostic Imaging / Q000379:methods; D006801:Humans; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D008297:Male; D011467:Prostate / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000175:diagnosis*; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D002788:Cholesterol Esters; D008055:Lipids; C007045:cholesteryl sulfate