title: Control of reduction thermodynamics in [2Fe-2S] ferredoxins Entropy-enthalpy compensation and the influence of surface mutations.
abstract: The reaction thermodynamics for the one-electron reduction of the [2Fe-2S] cluster of both human ferredoxin and various surface point mutants, in which each of the negatively charged residues Asp72, Glu73, Asp76, and Asp79 were converted to Ala, have been determined by variable temperature spectroelectrochemical measurements. The above are conserved residues that have been implicated in interactions between the vertebrate-type ferredoxins and their redox partners. In all cases, and similar to other 2Fe-ferredoxins, the reduction potentials are negative as a result of both an enthalpic and entropic stabilization of the oxidized state. Although all Hs Fd mutants, with the exception of Asp72Ala, show slightly higher E degrees ' values than that of wild type Hs Fd, according to expectations for a purely electrostatic model, they exhibit changes in the H degrees '(rc) values that are electrostatically counter-intuitive. The observation of enthalpy-entropy compensation within the protein series indicates that the mutation-induced changes in H degrees '(rc) and S degrees '(rc) are dominated by reduction-induced solvent reorganization effects. Protein-based entropic effects are likely to be responsible for the low E degrees ' value of D72A.
authors.affiliation: Department of Chemistry, University of Modena and Reggio Emilia, Via Campi, 183, 41100 Modena, Italy.
authors.forename: Marzia
authors.initials: M
authors.lastname: Bellei
authors.forename: Gianantonio
authors.initials: G
authors.lastname: Battistuzzi
authors.forename: Shu-pao
authors.initials: SP
authors.lastname: Wu
authors.forename: Sheref S
authors.initials: SS
authors.lastname: Mansy
authors.forename: James A
authors.initials: JA
authors.lastname: Cowan
authors.forename: Marco
authors.initials: M
authors.lastname: Sola
mesh_terms: D000595:Amino Acid Sequence; D004563:Electrochemistry; D019277:Entropy; D005288:Ferredoxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D013816:Thermodynamics
chemical_list: D005288:Ferredoxins; D007506:Iron-Sulfur Proteins