Clinical relevance of cagPAI intactness in Helicobacter pylori isolates from Vietnam.
The purpose of this paper is to investigate the relationship between clinical outcome and the intactness of cagPAI in Helicobacter pylori strains from Vietnam. The presence or absence of 30 cagPAI genes was investigated by polymerase chain reaction (PCR) and dot-blotting. H. pylori-induced interleukin-8 secretion and hummingbird phenotype, and H. pylori adhesion to gastric epithelial cells were examined. The serum concentration of pepsinogen 1, pepsinogen 2, and gastrin was also measured in all patients. cagPAI was present in all 103 Vietnamese H. pylori isolates, of which 91 had intact cagPAI and 12 contained only a part of cagPAI. Infection with the partial cagPAI strains was less likely to be associated with peptic ulcer and chronic gastric mucosal inflammation than infection with strains possessing intact cagPAI. The partial cagPAI strains lacked almost all ability to induce interleukin-8 secretion and the hummingbird phenotype in gastric cells. Their adhesion to epithelial cells was significantly decreased in comparison with intact cagPAI strains. Moreover, for the first time, we found an association between cagPAI status and the serum concentration of pepsinogens 1 and 2 in infected patients. H. pylori strains with internal deletion within cagPAI are less virulent and, thus, less likely to be associated with severe clinical outcomes.
Nguyen
L T
LT
Department of Molecular Pathology, Faculty of Medicine, Oita University, Yufu City, Oita, 879-5593, Japan.
Uchida
T
T
Tsukamoto
Y
Y
Trinh
T D
TD
Ta
L
L
Mai
H B
HB
Le
H S
HS
Ho
D Q D
DQ
Hoang
H H
HH
Matsuhisa
T
T
Okimoto
T
T
Kodama
M
M
Murakami
K
K
Fujioka
T
T
Yamaoka
Y
Y
Moriyama
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D005755:Gastrins / Q000097:blood; D044404:Genomic Islands; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020116:Pepsinogen A / Q000097:blood; D010437:Peptic Ulcer / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014744:Vietnam; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*; D055815:Young Adult
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005755:Gastrins; D016209:Interleukin-8; D037521:Virulence Factors; D020116:Pepsinogen A