title: Effect of sampling fat location and cooking on fatty acid composition of beef steaks.
abstract: To investigate the impact of sampling fat location and cooking on fatty acid composition of beef steaks, 21 raw steaks from crossbred steers were dissected to obtain outer (OSC) and inner subcutaneous fat (ISC), seam fat, marbling, and lean muscle. Twenty-one cooked steaks were dissected to obtain OSC, ISC, seam fat, surface and inner muscle. Trans-vaccenic acid and c9, t11-CLA percentages were lower (P<0.05) in lean muscle than subcutaneous (s.c.) fat or marbling. Monounsaturated: saturated fatty acid ratios were lower (P<0.05) in seam fat and marbling than s.c. fat or lean muscle. Linoleic and linolenic acid levels were highest in lean muscle and longer chain n-6 and n-3 fatty acids were only detected in lean muscle. Cooking did not change fatty acid composition dramatically except that n-6: n-3 ratio in s.c. and seam fat decreased after cooking (P<0.05).
authors.affiliation: Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USA.
authors.forename: T
authors.initials: T
authors.lastname: Jiang
authors.forename: J R
authors.initials: JR
authors.lastname: Busboom
authors.forename: M L
authors.initials: ML
authors.lastname: Nelson
authors.forename: J
authors.initials: J
authors.lastname: O'Fallon
authors.forename: T P
authors.initials: TP
authors.lastname: Ringkob
authors.forename: D
authors.initials: D
authors.lastname: Joos
authors.forename: K
authors.initials: K
authors.lastname: Piper
mesh_terms: D050153:Abdominal Fat / Q000033:anatomy & histology / Q000737:chemistry; D000818:Animals; D002417:Cattle; D003296:Cooking / Q000379:methods*; D004041:Dietary Fats / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D015525:Fatty Acids, Omega-3 / Q000032:analysis; D043371:Fatty Acids, Omega-6 / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D044243:Linoleic Acids, Conjugated / Q000032:analysis; D008297:Male; D008460:Meat / Q000032:analysis*; D009829:Oleic Acids / Q000032:analysis; D013045:Species Specificity; D050507:Subcutaneous Fat, Abdominal / Q000737:chemistry*
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D044243:Linoleic Acids, Conjugated; D009829:Oleic Acids; C503589:cis-9, trans-11-conjugated linoleic acid; C050413:11-octadecenoic acid