Determination of several potential virulence factors in non-o1 Vibrio cholerae, Pseudomonas aeruginosa, faecal coliforms and streptococci isolated from Marrakesh groundwater.
The dynamic, hemolytic and hemagglutination activities and the antibiotic resistance of non-O1 Vibrio cholerae, Pseudomonas aeruginosa, faecal coliforms (FC) and faecal streptococci (FS), isolated by standard membrane filtration methods from suburban and rural groundwater supplies, were carried out. Detectable non-O1 V. cholerae and P. aeruginosa was present in 81% and 88% of samples. The total occurrence of FC and FS during the period of study was 94%. The annual average densities of non-O1 V. cholerae were 4,903 MPN/100 mL. While, they were 206, 1,891 and 1,246 cfu/100 mL for P. aeruginosa, FC and FS respectively. Non-O1 V. cholerae strains had the highest percentage of hemolytic activities (alpha + beta) (71.29%), whereas 20.71% of FS, 16.88% of FC and 9.13% of P. aeruginosa strains produced hemolysin. Bacterial strains isolated were found to be adhesive, with percentages of 63.09%, 65.09%, 84.06% and 87.98% respectively for non-O1 V. cholerae, FS, FC and P. aeruginosa. As for antibiotic resistance, the overall resistance of non-O1 V. cholerae strains was 79%, whereas it was 100% for the other bacteria. Non-O1 V. cholerae resistance was expressed towards sulfamethoxazole (75%), streptomycin (62%) and cephalothin (60%). Obtained results indicated correlation between bacteriological pollution and their public health implications.
Lamrani Alaoui
Hafsa
H
Environmental Microbiology and Toxicology Unit, Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, P.O. 2390, Marrakesh 40000, Morocco.
Oufdou
Khalid
K
Mezrioui
Nour-Eddine
NE
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000472:pathogenicity*; D024881:Drug Resistance, Bacterial; D009018:Morocco; D037521:Virulence Factors / Q000737:chemistry*; D014871:Water Microbiology / Q000592:standards*; D014881:Water Supply / Q000592:standards*
D000900:Anti-Bacterial Agents; D037521:Virulence Factors