Organ-specific responses of vacuolar H-ATPase in the shoots and roots of C halophyte Suaeda salsa to NaCl.
Suaeda salsa L. is a halophytic species that is well adapted to high salinity. In order to understand its salt tolerance mechanism, we examined the growth and vacuolar H(+)-ATPase (V-ATPase) response to NaCl within the shoots and roots. The growth of shoots, but not roots, was dramatically stimulated by NaCl. Cl(-) and Na(+) were mainly accumulated in shoots. V-ATPase activity was significantly increased by NaCl in roots and especially in shoots. Interestingly, antisera ATP95 and ATP88b detected three V(1) subunits (66, 55 and 36 KDa) of V-ATPase only in shoots, while an 18 kDa V(0) subunit of V-ATPase was detected by both antisera in shoots and roots. It suggested that the tissue-specific characteristics of V-ATPase were related to the different patterns of growth and ion accumulation in shoots and roots of S. salsa.
Yang
Ming-Feng
MF
Key Laboratory of Plant Stress Research, College of Life Sciences, Shandong Normal University, Jinan 250014, China.
Song
Jie
J
Wang
Bao-Shan
BS
D018533:Biomass; D015153:Blotting, Western; D019663:Chenopodiaceae / Q000187:drug effects* / Q000201:enzymology*; D002712:Chlorides / Q000378:metabolism; D003429:Cross Reactions / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D009928:Organ Specificity / Q000187:drug effects; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D018520:Plant Shoots / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D021122:Protein Subunits / Q000276:immunology; D055051:Salt-Tolerant Plants / Q000187:drug effects / Q000201:enzymology*; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D025262:Vacuolar Proton-Translocating ATPases / Q000276:immunology / Q000378:metabolism*; D014617:Vacuoles / Q000187:drug effects / Q000201:enzymology
D002712:Chlorides; D021122:Protein Subunits; D012965:Sodium Chloride; D012964:Sodium; D025262:Vacuolar Proton-Translocating ATPases