Three-dimensional surface patterning by DNA-modifying enzymes.
Self-assembled patterned multilayers may be fabricated using DNA monolayers and the orchestrated reactions of DNA-modifying enzymes. To demonstrate this approach, DNA monolayers were formed on silicon and cleaved quantitatively with a restriction enzyme. Subsequently, fluorescently labeled nucleotides were covalently incorporated to the cleaved DNA. Nucleotide addition was shown to be highly selective according to the sequence at the cleavage site, and no nonspecific adsorption to the surface was observed. The dual action of the DNA-modifying enzymes was quantitative and could be utilized in the fabrication of multilayered structures. Other DNA-modifying enzymes can be exploited in this manner to enrich the repertoire of self-assembly supramolecular structure fabrication.
Kaufmann
R
R
Department of Chemical Physics and Chemical Research Support, Weizmann Institute of Science, Rehovot 76100, Israel.
Peled
D
D
Naaman
R
R
Daube
S S
SS
D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism*; D012825:Silicon; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D009711:Nucleotides; D004247:DNA; D004262:DNA Restriction Enzymes; D012825:Silicon