Chemical synthesis of homogeneous glycopeptides and glycoproteins.
Oligosaccharides linked to proteins are known to play important roles in several biological events. However, oligosaccharides are heterogeneous, which has hindered detailed elucidation of oligosaccharide functions. In order to solve this problem, glycoproteins having homogeneous oligosaccharides have long been required. For this purpose, an efficient preparative method of complex-type oligosaccharides has been investigated from a natural source and this method was found to afford over 24 kinds of diverse complex-type oligosaccharides by use of chemical methods and branch-specific sequential glycosidase digestion. The sufficient amount of homogeneous complex type oligosaccharides obtained enabled us to examine the synthesis of homogeneous glycopeptides as well as glycoproteins by use of solid phase glycopeptide synthetic method and native chemical ligation. This review describes recent progress related to the efficient method of oligosaccharide preparation and synthesis of glycoproteins including bioactive erythropoietin.
Kajihara
Yasuhiro
Y
International Graduate School of Arts and Sciences, Yokohama City University, 22-2 Seto; Kanazawaku, Yokohama, 236-0027 Japan. kajihara@chem.sci.osaka-u.ac.jp
Yamamoto
Naoki
N
Okamoto
Ryo
R
Hirano
Kiriko
K
Murase
Takefumi
T
D000595:Amino Acid Sequence; D000818:Animals; D002621:Chemistry / Q000379:methods*; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D006023:Glycoproteins / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data
D006020:Glycopeptides; D006023:Glycoproteins