The molecular basis for water taste in Drosophila.
The detection of water and the regulation of water intake are essential for animals to maintain proper osmotic homeostasis. Drosophila and other insects have gustatory sensory neurons that mediate the recognition of external water sources, but little is known about the underlying molecular mechanism for water taste detection. Here we identify a member of the degenerin/epithelial sodium channel family, PPK28, as an osmosensitive ion channel that mediates the cellular and behavioural response to water. We use molecular, cellular, calcium imaging and electrophysiological approaches to show that ppk28 is expressed in water-sensing neurons, and that loss of ppk28 abolishes water sensitivity. Moreover, ectopic expression of ppk28 confers water sensitivity to bitter-sensing gustatory neurons in the fly and sensitivity to hypo-osmotic solutions when expressed in heterologous cells. These studies link an osmosensitive ion channel to water taste detection and drinking behaviour, providing the framework for examining the molecular basis for water detection in other animals.
Cameron
Peter
P
Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute, CA, USA.
Hiroi
Makoto
M
Ngai
John
J
Scott
Kristin
K
D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal / Q000502:physiology; D002460:Cell Line; D004326:Drinking / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D053503:Epithelial Sodium Channels / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D009994:Osmolar Concentration; D011984:Sensory Receptor Cells / Q000378:metabolism*; D013649:Taste / Q000502:physiology*; D014867:Water
D029721:Drosophila Proteins; D053503:Epithelial Sodium Channels; C549272:PPK28 protein, Drosophila; D014867:Water