Alternation of gene expression in trigeminal ganglion neurons following complete Freund's adjuvant or capsaicin injection into the rat face.
The hyperexcitability of trigeminal ganglion (TG) neurons following inflammation or C-fiber stimulation is known to be involved in a variety of changes in gene expression in TG neurons, resulting in pain abnormalities in orofacial regions. We analyzed nocifensive behavior following complete Freund's adjuvant (CFA) or capsaicin injection into the maxillary whisker pad, and gene expression in the TG neurons using microarray analysis. The head-withdrawal latency to capsaicin injection or the head-withdrawal threshold to mechanical stimulation of the whisker pad skin in CFA-treated rats was significantly decreased compared to vehicle-treated rats. Many up-regulated and down-regulated genes in the TG neurons of each model were reported. Genes which have not been linked to peripheral inflammation or C-fiber activation were detected. Moreover, microarray chip containing a number of non-coding sequences was also up-regulated by C-fiber activation. These findings suggest that the diverse gene expressions in TG neurons are differentially involved in the inflammatory chronic pain and the acute pain induced by C-fiber activation, and the hyperexcitation of C-fibers are associated with the activation of certain non-coding RNAs.
Okumura
Masayo
M
Institute for Oral Science, Matsumoto Dental University, 1780, Hiro-oka, Gobara, Shiojiri, Nagano 399-0781, Japan. okmrm@po.mdu.ac.jp
Iwata
Koichi
K
Yasuda
Koichi
K
Inoue
Katsuhiro
K
Shinoda
Masamichi
M
Honda
Kuniya
K
Shibuta
Kazuo
K
Yasuda
Masashi
M
Kondo
Eiji
E
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D002211:Capsaicin / Q000494:pharmacology*; D004195:Disease Models, Animal; D005145:Face / Q000294:innervation; D005157:Facial Pain / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D005620:Freund's Adjuvant / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D036421:Nerve Fibers, Unmyelinated / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010147:Pain Measurement / Q000379:methods; D017288:Pain Threshold / Q000187:drug effects / Q000502:physiology; D022661:RNA, Untranslated / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000378:metabolism*; D018689:Sensory System Agents / Q000494:pharmacology; D012668:Trigeminal Ganglion / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D014738:Vibrissae / Q000294:innervation
D000276:Adjuvants, Immunologic; D009419:Nerve Tissue Proteins; D022661:RNA, Untranslated; D018689:Sensory System Agents; D005620:Freund's Adjuvant; D002211:Capsaicin