title: Lineage pattern, trans-species polymorphism, and selection pressure among the major lineages of feline MHC-DRB peptide-binding region.
abstract: The long-term evolution of major histocompatibility complex (MHC) involves the birth-and-death process and independent divergence of loci during episodes punctuated by natural selection. Here, we investigated the molecular signatures of natural selection at exon-2 of MHC class II DRB gene which includes a part of the peptide-binding region (PBR) in seven of eight putative extant Felidae lineages. The DRB alleles in felids can be mainly divided into five lineages. Signatures of trans-species polymorphism among major allelic lineages indicate that balancing selection has maintained the MHC polymorphism for a long evolutionary time. Analysis based on maximum likelihood models of codon substitution revealed overall purifying selection acting on the feline DRB. Sites that have undergone positive selection and those that are under divergent selective pressure among lineages were detected and found to fall within the putative PBR. This study increased our understanding of the nature of selective forces acting on DRB during feline radiation.
authors.affiliation: Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Sichuan, 610064, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wei
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Wang
authors.forename: Wenping
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Xu
authors.forename: Fujun
authors.initials: F
authors.lastname: Shen
authors.forename: Bisong
authors.initials: B
authors.lastname: Yue
mesh_terms: D000818:Animals; D019143:Evolution, Molecular; D045989:Felidae / Q000145:classification / Q000235:genetics*; D005802:Genes, MHC Class II; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012641:Selection, Genetic