Tetrasomy 8 in a patient with chronic lymphocytic leukemia.
We report a case of a 47-year-old man diagnosed with chronic lymphocytic leukemia (CLL) with two extra copies of chromosome 8. Classical cytogenetic analysis by the immunostimulatory combination of DSP30 and interleukin 2 showed tetrasomy of chromosome 8 in 60% of the metaphase cells (48,XY,+8,+8[12]/46,XY[8]). Spectral karyotype analysis confirmed the abnormality previously seen by G banding. Additionally, interphase fluorescence in situ hybridization using an LSI CEP 8 probe performed on peripheral blood cells without any stimulant agent showed tetrasomy of chromosome 8 in 54% of analyzed cells (108 of 200). To our knowledge, tetrasomy 8 as the sole chromosomal abnormality in CLL has not been previously described. The prognostic significance of tetrasomy 8 in CLL remains to be elucidated. However, the patient has been followed up in the outpatient hospital since 2004 without any therapeutic intervention and has so far remained stable.
de Oliveira
Fábio Morato
FM
Department of Internal Medicine, Division of Hematology, School of Medicine of Ribeirão Preto, University of São Paulo, 14049-900 Ribeirão Preto, SP, Brazil. fabiomorato@usp.br
Brandão
Renata Amorim
RA
Leite-Cueva
Sabrina Dias
SD
de Paula Careta
Francisco
F
Simões
Belinda Pinto
BP
Rego
Eduardo Magalhães
EM
Falcão
Roberto Passetto
RP
D002869:Chromosome Aberrations; D002898:Chromosomes, Human, Pair 8; D020732:Cytogenetic Analysis; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011123:Polyploidy