title: Evaluation of subcutaneous and alveolar implantation surgical sites in the study of the biological properties of root-end filling endodontic materials.
abstract: OBJECTIVE
  The aim of this study was to compare two methodologies used in the evaluation of tissue response to root-end filling materials in rats.
  
  
  METHODS
  Forty rats were divided into 4 groups: in Groups I and II (control groups), empty polyethylene tubes were implanted in the extraction site and in the subcutaneous tissue, respectively; in Groups III and IV, polyethylene tubes filled with ProRoot MTA were implanted in the extraction site and in the subcutaneous tissue, respectively. The animals were killed 7 and 30 days after tube implantation, and the hemi-maxillas and the capsular subcutaneous tissue, both with the tubes, were removed. Specimens were processed and evaluated histomorphologicaly under light microscopy. The scores obtained were analyzed statistically by the Kruskal-Wallis test (p<0.05).
  
  
  RESULTS
  There were no statistically significant differences between the implantation methods (p=0.78033, p=0.72039). It was observed that the 30-day groups presented a more mature healing process due to smaller number of inflammatory cells.
  
  
  CONCLUSIONS
  The present study showed no differences in tissue responses as far as the implantation site and the studied period were concerned. Alveolar socket implantation methodology represents an interesting method in the study of the biological properties of root-end filling endodontic materials due to the opportunity to evaluate bone tissue response.
authors.affiliation: Department of Restorative Dentistry, Maringá Dental School, UNINGA University, Maringá, PR, Brazil. lucianocintra@hotmail.com.br
authors.forename: Luciano Tavares Angelo
authors.initials: LT
authors.lastname: Cintra
authors.forename: Pedro Felício Estrada
authors.initials: PF
authors.lastname: Bernabé
authors.forename: Ivaldo Gomes
authors.initials: IG
authors.lastname: de Moraes
authors.forename: João Eduardo
authors.initials: JE
authors.lastname: Gomes-Filho
authors.forename: Tetuo
authors.initials: T
authors.lastname: Okamoto
authors.forename: Alberto
authors.initials: A
authors.lastname: Consolaro
authors.forename: Tiago Novaes
authors.initials: TN
authors.lastname: Pinheiro
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology; D000539:Alveolar Process / Q000187:drug effects* / Q000473:pathology; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology; D003238:Connective Tissue / Q000473:pathology; D004338:Drug Combinations; D006097:Granulation Tissue / Q000473:pathology; D006171:Gutta-Percha / Q000494:pharmacology; D007249:Inflammation; D008422:Materials Testing; D018919:Neovascularization, Physiologic / Q000502:physiology; D010087:Oxides / Q000494:pharmacology; D020959:Polyethylene / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012184:Retrograde Obturation; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology; D040521:Subcutaneous Tissue / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors; D020390:Tooth Socket / Q000187:drug effects* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D006171:Gutta-Percha; D020959:Polyethylene