Chemical modification of hydrophobic block in poly(ethylene oxide) poly(caprolactone) based nanocarriers: effect on the solubilization and hemolytic activity of amphotericin B.
The aim of this study was to develop methoxy poly(ethylene oxide)-b-poly(epsilon-caprolactone) (MePEO-b-PCL) containing stearyl (St) substituents on PCL, and assess the efficacy of nanocarriers formed from this structure in comparison to unmodified MePEO-b-PCL and those with carboxyl substitutes on PCL on the solubilization and delivery of Amphotericin B (AmB). Prepared block copolymers were characterized for their molecular weight by (1)H NMR and gel permeation chromatography. The self-assembly of synthesized MePEO-b-PStCL to spherical particles of nanometer size range was shown by dynamic light scattering as well as electron and atomic force microscopy. Encapsulated AmB showed a reduction in its hemolytic activity against rat red blood cells in comparison to the commercial formulation of AmB, Fungizone.
Falamarzian
Arash
A
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada.
Lavasanifar
Afsaneh
A
D000666:Amphotericin B / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D002850:Chromatography, Gel; D004337:Drug Carriers / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects*; D057927:Hydrophobic and Hydrophilic Interactions; D007783:Lactones; D009682:Magnetic Resonance Spectroscopy; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols; D051381:Rats; D012995:Solubility
D004337:Drug Carriers; D007783:Lactones; C473831:poly(ethylene oxide)-b-poly(caprolactone); D011092:Polyethylene Glycols; D000666:Amphotericin B