title: Detection of circulating tumor cells in peripheral blood from patients with gastric cancer using microRNA as a marker.
abstract: Recently, the detection of occult cancer cells in peripheral blood has received a great deal of attention regarding the prediction of postoperative cancer recurrence and for novel strategies of adjuvant therapy. The aim of this study was to establish a new molecular diagnostic method of detecting circulating tumor cells. Gastric cancer SGC-7901 cells in 2 ml blood from healthy volunteers were serially diluted. Additional peripheral blood samples were collected from 90 patients and 27 healthy volunteers. Real-time reverse transcription-polymerase chain reaction was used to detect the levels of microRNA-106a (miR-106a) and microRNA-17 (miR-17). Receiver operating characteristics (ROC) curves were constructed. In recovery experiments, a significant correlation between the number of cancer cells and the levels of both miR-106a (r = -0.906, p = 0.037) and miR-17 (r = -0.912, p = 0.031) was found. In preoperative and postoperative patient groups, miR-106a and miR-17 levels were significantly higher than those in controls. The areas under the ROC curve for miR-106a, miR-17, and combination were 0.684 (p = 0.0066), 0.743 (p = 0.0001), and 0.741 (p = 0.0002), respectively. Our results indicate that the detection of miRNA in peripheral blood may be a novel tool for monitoring circulating tumor cells in patients with gastric cancers.
authors.affiliation: Ningbo University School of Medicine, Ningbo 315211, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Jun-Ming
authors.initials: JM
authors.lastname: Guo
authors.forename: Yan-Ru
authors.initials: YR
authors.lastname: Lou
authors.forename: Xin-Jun
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Fa-De
authors.initials: FD
authors.lastname: Zhong
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jiang
authors.forename: Jia
authors.initials: J
authors.lastname: Cheng
authors.forename: Bing-Xiu
authors.initials: BX
authors.lastname: Xiao
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D045744:Cell Line, Tumor; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating; D012372:ROC Curve; D013274:Stomach Neoplasms / Q000097:blood / Q000235:genetics / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C542541:MIRN106 microRNA, human; C524941:MIRN17 microRNA, human; D035683:MicroRNAs