Determination of glyphosate and its metabolites in plant material by reversed-polarity CE with indirect absorptiometric detection.
A simple CE method for simultaneous determination of glyphosate and its metabolites (i.e. aminomethylphosphonic acid, glyoxylate, sarcosine and formaldehyde) in plants is reported here. A BGE of pH 7.5, 10% ACN, 7.5 mM phthalate, containing 0.75 mM hexadecyltrimethylammonium bromide as an electro-osmotic flow modifier, an applied voltage of -20 kV and absorptiometric monitoring at 220 nm were the optimal chemical and instrumental parameters. The method, with development time 20 min, shows linear calibrations within the range 5-500 microg/mL (for all target analytes) with correlation coefficients between 0.999 and 0.998. It has been validated by application to samples of Lolium spp. The electroinjection mode hinders most interferents to enter the capillary, thus providing a clean electropherogram and making unnecessary long sample-preparation steps.
Rojano-Delgado
Antonia María
AM
Department of Agricultural Chemistry, Campus of Rabanales, University of Córdoba, Córdoba, Spain.
Ruiz-Jiménez
José
J
de Castro
María Dolores Luque
MD
De Prado
Rafael
R
D000096:Acetone / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D006038:Glyoxylates / Q000032:analysis / Q000378:metabolism; D006540:Herbicides / Q000032:analysis* / Q000378:metabolism; D016014:Linear Models; D008129:Lolium / Q000737:chemistry* / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry* / Q000378:metabolism; D015203:Reproducibility of Results; D012521:Sarcosine / Q000032:analysis / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014867:Water / Q000737:chemistry; D000097797:Glyphosate
D000096:Acetone; D005998:Glycine; D006038:Glyoxylates; D006540:Herbicides; D000432:Methanol; D012521:Sarcosine; D014867:Water; D000097797:Glyphosate; C031150:glyoxylic acid