title: Investigations on the migration behavior of insulin and related synthetic analogues in CZE, MEKC and MEEKC employing different surfactants.
abstract: The retention/migration behavior of insulin and five synthetic insulin analogues in CZE, MEKC and MEEKC employing seven different detergents within the latter two techniques has been investigated. Substantial changes in separation selectivity in MEKC could be observed for several insulins when moving from SDS to cholate-based micellar systems. Customized separations could be achieved by using mixtures of SDS and deoxycholate. A similar effect could be observed in MEEKC although the overall quality of MEEKC separations was inferior to those obtained with MEKC.
authors.affiliation: Institute of Analytical Chemistry, Johannes-Kepler-University, Linz, Austria.
authors.forename: Manuela
authors.initials: M
authors.lastname: Haunschmidt
authors.forename: Karin
authors.initials: K
authors.lastname: Ortner
authors.forename: Katharina
authors.initials: K
authors.lastname: Hainz
authors.forename: Elke
authors.initials: E
authors.lastname: Bradt
authors.forename: Lukas
authors.initials: L
authors.lastname: Sternbauer
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Buchberger
authors.forename: Christian W
authors.initials: CW
authors.lastname: Klampfl
mesh_terms: D000595:Amino Acid Sequence; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003840:Deoxycholic Acid / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004655:Emulsions; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry*; D007328:Insulin / Q000031:analogs & derivatives* / Q000737:chemistry*; D008969:Molecular Sequence Data; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D004655:Emulsions; D007004:Hypoglycemic Agents; D007328:Insulin; D013501:Surface-Active Agents; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate