title: Protection against direct in-contact challenge following foot-and-mouth disease vaccination in sheep and goats: the effect on virus excretion and carrier status.
abstract: The ability of foot-and-mouth disease (FMD) vaccine to protect sheep and goats from a homologous direct in-contact challenge and the effect on virus excretion from the nasal secretions and oropharynx was examined. An experimental oil adjuvant O(1) Manisa FMD vaccine protected sheep and goats from clinical disease from 7 days post vaccination following 24 hours of direct in-contact exposure to four infected donor sheep or goats. Goats required lower antibody titres for protection when compared with sheep. Protection from clinical disease did not prevent localized viral replication in goats and at least two goats had viral RNA detected on day 28 post challenge. Quantitative reverse transcriptase polymerase chain reaction showed that the level of virus replication shortly after direct in-contact challenge in oropharynx and nasal secretions of vaccinated animals was reduced by 100 and 1000 times respectively when compared with unvaccinated controls. The findings also show that after direct in-contact challenge, use of FMD vaccine will prevent or reduce local virus replication, thereby significantly reduce the amount of virus released into the environment in the all-important early post-exposure period. There is low risk of vaccinated animals transmitting disease as live virus could not be readily isolated.
authors.affiliation: Foot-and-Mouth Disease Virus Laboratory, Research and Development Centre, Indian Immunologicals Limited, Gachibowli, Hyderabad, India.
authors.forename: Muthukrishnan
authors.initials: M
authors.lastname: Madhanmohan
authors.forename: Singanallur Balasubramanian
authors.initials: SB
authors.lastname: Nagendrakumar
authors.forename: Villuppanoor Alwar
authors.initials: VA
authors.lastname: Srinivasan
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000821:virology; D005260:Female; D005536:Foot-and-Mouth Disease / Q000517:prevention & control* / Q000635:transmission; D015511:Goat Diseases / Q000517:prevention & control*; D006041:Goats; D008297:Male; D009093:Mucus / Q000821:virology; D012756:Sheep; D012757:Sheep Diseases / Q000517:prevention & control*; D014611:Vaccination; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D014765:Viral Vaccines