title: Voltammetric determination of sulfamethoxazole at a multiwalled carbon nanotube modified glassy carbon sensor and its application studies.
abstract: Sulfamethoxazole (SMX) is an anti-bacterial sulfonamide. It prevents the formation of dihydrofolic acid, a compound that bacteria need in order to survive. The present work details the voltammetric analysis of SMX at a multiwalled carbon nanotube (MWCNT)-Nafion modified glassy carbon electrode (GCE). Sulfamethoxazole gives a well defined oxidation peak at 0.74 V in 0.1 M phosphate buffer solution (PBS) of pH 8.0. The experimental parameters such as the amount of MWCNT-Nafion suspension, the pH of the supporting electrolyte and scan rate were optimized and a direct electrochemical method for the determination of SMX was developed. Under optimum conditions the oxidation peak current is linear to the concentration of SMX in the range 1 x 10(-2) - 5 x 10(-5) M with a detection limit of 1 x 10(-5) M. The MWCNT/GCE showed good stability, selectivity and was successfully used to quantify SMX in pharmaceutical formulations and urine sample.
authors.affiliation: Department of Applied Chemistry, Cochin University of Science and Technology Kochi, India.
authors.forename: Sindhu
authors.initials: S
authors.lastname: Issac
authors.forename: K
authors.initials: K
authors.lastname: Girish Kumar
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006801:Humans; D066298:In Vitro Techniques; D037742:Nanotubes, Carbon; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013420:Sulfamethoxazole / Q000032:analysis* / Q000737:chemistry / Q000652:urine
chemical_list: D000890:Anti-Infective Agents; D037742:Nanotubes, Carbon; D013420:Sulfamethoxazole