[Effects of culture conditions on biomass and active components of adventitious roots culture in Panax ginseng].
OBJECTIVE
To optimize the culture condition of adventitious roots of Panax ginseng.


METHODS
The adventitious roots were obtained through tissue culture by manipulation of inoculum, various sucrose concentrations and salt strength. The contents of ginsenosides Re, Rb1 and Rg1 were determined by HPLC while the contents of polysaccharides were determined by ultraviolet spectrophotometry.


RESULTS
The multiplication of adventitious roots reached the peak when the inoculum was 20 g x L(-1). The effects of sucrose concentration and salt strength on adventitious roots were observed. The contents of polysaccharides were higher when the medium contained more sucrose. 40 g x L(-1) sucrose was favorable for roots growth and biosynthesis of Re, while 30 g x L(-1) was favorable for the biosynthesis of Rb1 and Rg1. 3/4MS medium was benefit for the growth of adventitious roots and the biosynthesis of ginsenosides. The contents of polysaccharides were decreased with the increase of salt strength.


CONCLUSIONS
The results showed that inoculum, various sucrose concentrations and salt strength have significant influences on adventitious roots growth, secondary metabolite and polysaccharide synthesis in P. ginseng.
Huang
Tao
T
College of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Gao
Wenyuan
W
Wang
Juan
J
Cao
Yu
Y
D003470:Culture Media / Q000494:pharmacology*; D005894:Panax / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media