Sinosporangium album gen. nov., sp. nov., a new member of the suborder Streptosporangineae.
A Gram-positive, aerobic, non-motile actinobacterium, designated strain 6014(T), was isolated from a soil sample collected from Qinghai province, north-west China, and subjected to a polyphasic taxonomic study. The isolate formed elementary branching hyphae and abundant aerial mycelia with globose sporangia on ISP 4 and R2A media. Whole-cell hydrolysates of strain 6014(T) contained arabinose, galactose and ribose as diagnostic sugars and meso-diaminopimelic acid as the diagnostic diamino acid. The polar lipids consisted of phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, N-acetylglucosamine-containing phospholipids, two unknown phospholipids and an unknown glycolipid. The menaquinone system contained MK-9(H₂) and MK-9(H₄). The major fatty acids were C₁₄:₀, i-C₁₅:₀, C₁₆:₀ and 10-methyl-C₁₆:₁. The genomic DNA G+C content of the isolate was 69.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 6014(T) fell within the radius of the suborder Streptosporangineae, in which the strain formed a distinct lineage next to genera of the family Streptosporangiaceae. Based on data from this polyphasic study, strain 6014(T) can be readily distinguished from previously described organisms and represents a member of a novel species within a new genus in the suborder Streptosporangineae. The name Sinosporangium album gen. nov., sp. nov. is proposed with 6014(T) (=DSM 45181(T) =KCTC 19655(T)) as the type strain.
Zhang
Yu-Qin
YQ
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
Liu
Hong-Yu
HY
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
Yu
Li-Yan
LY
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
Lee
Jae-Chan
JC
Biological Resource Center, Korea Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
Park
Dong-Jin
DJ
Biological Resource Center, Korea Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
Kim
Chang-Jin
CJ
Biological Resource Center, Korea Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
Xu
Li-Hua
LH
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Jiang
Cheng-Lin
CL
Biological Resource Center, Korea Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
Li
Wen-Jun
WJ
Biological Resource Center, Korea Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000596:Amino Acids / Q000032:analysis; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
D000596:Amino Acids; D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2