[Prophylactic protective effects of paeoniflorin on corticosterone-induced primary cultures of rat cortical neurons damages].
OBJECTIVE
To observe the prophylactic neuroprotective effect of paeoniflorin (PF) on corticosterone-induced primary cultures of rat cortical neurons damages.


METHODS
Primary cultures of neurons from the of 16-days-old embryo rats were used to study. Neurons in the experimental group were treated with cort (200 micromol x L(-1)) 30 min after the pretreatment of PF (0.5, 2, 10 micromol x L(-1)). We used MTT assay to detect the action of paeoniflorin on neuronal activity. DAPI staining to study of the influence of PF on neuronal apoptosis morphology, and Annexin V-FITC/PI apoptosis detection kit to inspect the impact of paeoniflorin on the early neuronal apoptosis.


RESULTS
Compared with model group, every dose of PF (0.5, 2, 10 micromol x L(-1)) can raise the neuronal activity notably (P<0.05), PF of 2 micromol x L(-1) and 10 micromol x L(-1) can reduce the rate of neuronal apoptosis.


CONCLUSIONS
Our data indicated that PF achieved prophylactic neuroprotective effect through against corticosterone-induced neurons damages.
Liu
Yang
Y
Tianjin University of Traditional Chinese Medicine, Tianjin Laboratory of Chinese Medicine Pharmacology, Tianjin 300193, China. liu8042222@163.com
Cui
Guangzhi
G
Zhang
Yanjun
Y
Gao
Xiumei
X
Gao
Yan
Y
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001565:Benzoates / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D003345:Corticosterone / Q000494:pharmacology*; D005260:Female; D005960:Glucosides / Q000494:pharmacology*; D008297:Male; D039821:Monoterpenes; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D003345:Corticosterone; D005960:Glucosides; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides