Pooled analysis of studies on DNA adducts and dietary vitamins.
OBJECTIVE
There is some evidence that dietary components that are rich in antioxidant and vitamins are inversely associated with DNA adduct levels induced by environmental carcinogens such as polycyclic aromatic hydrocarbons, although the epidemiologic data are inconsistent. This study addresses the association between vitamins, DNA adducts and smoking.


METHODS
A combined analysis of individual data on the association between bulky DNA adducts and dietary vitamins was conducted. A Medline search was performed to identify studies on healthy subjects in which smoking and vitamins intake information were available, and bulky DNA adducts were measured in peripheral blood with 32P-postlabelling. Eight published studies met the eligibility criteria, and individual data from 7 data sets including 2758 subjects were obtained. GSTM1 and GSTT1 were also available on all the subjects.


RESULTS
Vitamin E was inversely significantly associated with DNA adducts after adjustment for possible confounding factors. Vitamins A and C were not independent predictors of DNA adducts. A stratified analysis showed that vitamin A had a significant inverse association with DNA adducts in ever smokers only.


CONCLUSIONS
This result is relevant to planning any future chemo-preventive interventions directed to high risk subgroups of the population, for cancer prevention.
Ragin
Camille
C
SUNY Downstate Medical Center, Brooklyn, NY, USA.
Minor
Aerie
A
University of Pittsburgh Cancer Center, Pittsburgh, PA, USA.
Agudo
Antonio
A
Catalan Institute of Oncology (ICO), Barcelona, Spain.
Farmer
Peter
P
Cancer Biomarkers and Prevention Group, Biocentre, University of Leicester, UK.
Garte
Seymour
S
Genetics Research Institute ONLUS, Milan, Italy.
Gonzales
Carlos
C
Catalan Institute of Oncology (ICO), Barcelona, Spain.
Kalina
Ivan
I
Department of Medical Biology, Medical Faculty University P.J. Šafárik, Košice, Slovak Republic.
Matullo
Giuseppe
G
ISI Foundation, Turin, Italy.
Popov
Todor
T
National Center of Public Health Protection, Sofia, Bulgaria.
Palli
Domenico
D
Cancer Risk Factor Branch, ISPO-Cancer Prevention and Research Institute, Florence, Italy.
Peluso
Marco
M
Cancer Risk Factor Branch, ISPO-Cancer Prevention and Research Institute, Florence, Italy.
Ricceri
Fulvio
F
ISI Foundation, Turin, Italy.
Sram
Radim
R
Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine of Academy AS CR and Health Institute of Central Bohemia, Prague, Czech Republic.
Vineis
Paolo
P
Imperial College London, London, UK; University of Turin, Turin, Italy.
Taioli
Emanuela
E
SUNY Downstate Medical Center, Brooklyn, NY, USA. Electronic address: emanuela.taioli@downstate.edu.
D000367:Age Factors; D000975:Antioxidants / Q000032:analysis; D002273:Carcinogens / Q000633:toxicity; D018736:DNA Adducts; D004032:Diet; D004781:Environmental Exposure; D006801:Humans; D012907:Smoking; D014815:Vitamins / Q000097:blood
D000975:Antioxidants; D002273:Carcinogens; D018736:DNA Adducts; D014815:Vitamins
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