Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography.
Six volatile compounds, curdione (1), curcumol (2), germacrone (3), curzerene (4), 1,8-cineole (5) and beta-elemene (6), were successfully isolated from the essential oil of Curcuma wenyujin by high-performance centrifugal partition chromatography using a nonaqueous two-phase solvent system consisting of petroleum ether-acetonitrile-acetone (4:3:1 v/v/v). A total of 8 mg of curdione (1), 4 mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), 9 mg of 1,8-cineole (5) and 17 mg of beta-elemene (6) were isolated from the essential oil (300 mg) in 500 min. Their structures were determined by comparison of their retention times and MS data with those of the authentic samples as well as NMR spectroscopic analysis.
Dang
Yuan-Ye
YY
Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, P. R. China.
Li
Xiao-Cen
XC
Zhang
Qing-Wen
QW
Li
Shao-Ping
SP
Wang
Yi-Tao
YT
D002498:Centrifugation; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D030024:Curcuma / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008956:Models, Chemical; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
D009822:Oils, Volatile; D010936:Plant Extracts; D012997:Solvents