Ixodes ricinus tick saliva modulates tick-borne encephalitis virus infection of dendritic cells.
Tick-borne encephalitis virus is an important human pathogen, naturally delivered into host skin via a tick bite. To examine the effects of the virus on dendritic cell biology, we cultured dendritic cells with two tick-borne encephalitis virus strains of different virulence in the presence of Ixodes ricinus tick saliva. Tick saliva treatment increased proportion of virus-infected cells, led to a decrease in virus-induced TNF-alpha and IL-6 production and to reduced virus-induced apoptosis. Our data indicate that tick saliva modulate virus-mediated alterations in dendritic cells, thus probably being involved in the early infection process in the host.
Fialová
Anna
A
Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branisovská 31, CZ-37005 Ceské Budejovice, Czech Republic. anna.fialova@lfmotol.cuni.cz <anna.fialova@lfmotol.cuni.cz>
Cimburek
Zdenek
Z
Iezzi
Giandomenica
G
Kopecký
Jan
J
D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000821:virology*; D004669:Encephalitis Viruses, Tick-Borne / Q000472:pathogenicity*; D005260:Female; D015850:Interleukin-6 / Q000378:metabolism; D018884:Ixodes / Q000821:virology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012463:Saliva / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha