title: Preoperative prediction of hepatocellular carcinoma tumour grade and micro-vascular invasion by means of artificial neural network: a pilot study.
abstract: OBJECTIVE
  Hepatocellular carcinoma (HCC) prognosis strongly depends upon nuclear grade and the presence of microscopic vascular invasion (MVI). The aim of this study was to develop an artificial neural network (ANN) that is able to predict tumour grade and MVI on the basis of non-invasive variables.
  
  
  METHODS
  Clinical, radiological, and histological data from 250 cirrhotic patients resected (n=200) or transplanted (n=50) for HCC were analyzed. ANN and logistic regression models were built on a training group of 175 randomly chosen patients and tested on the remaining testing group of 75. Receiver operating characteristics curve (ROC) and k-statistics were used to analyze model accuracy in the prediction of the final histological assessment of tumour grade (G1-G2 vs. G3-G4) and MVI (absent vs. present).
  
  
  RESULTS
  Pathologic examination showed G3-G4 in 69.6% of cases and MVI in 74.4%. Preoperative serum alpha-fetoprotein (AFP), tumour number, size, and volume were related to tumour grade and MVI (p<0.05) and were used for ANN building, whereas, tumour number did not enter into the logistic models. In the training group, ANN area under ROC curves (AUC) for tumour grade and MVI prediction were 0.94 and 0.92, both higher (p<0.001) than those of logistic models (0.85 for both). In the testing group, ANN correctly identified 93.3% of tumour grades (k=0.81) and 91% of MVI (k=0.73). Logistic models correctly identified 81% of tumour grades (k=0.55) and 85% of MVI (k=0.57).
  
  
  CONCLUSIONS
  ANN identifies HCC tumour grades and MVI on the basis of preoperative variables more accurately than the conventional linear model and should be used for tailoring clinical management.
authors.affiliation: Liver and Multiorgan Transplant Unit, Policlinico S.Orsola-Malpighi, University of Bologna, Italy. aleqko@libero.it
authors.forename: Alessandro
authors.initials: A
authors.lastname: Cucchetti
authors.forename: Fabio
authors.initials: F
authors.lastname: Piscaglia
authors.forename: Antonia D'Errico
authors.initials: AD
authors.lastname: Grigioni
authors.forename: Matteo
authors.initials: M
authors.lastname: Ravaioli
authors.forename: Matteo
authors.initials: M
authors.lastname: Cescon
authors.forename: Matteo
authors.initials: M
authors.lastname: Zanello
authors.forename: Gian Luca
authors.initials: GL
authors.lastname: Grazi
authors.forename: Rita
authors.initials: R
authors.lastname: Golfieri
authors.forename: Walter Franco
authors.initials: WF
authors.lastname: Grigioni
authors.forename: Antonio Daniele
authors.initials: AD
authors.lastname: Pinna
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006528:Carcinoma, Hepatocellular / Q000473:pathology* / Q000601:surgery; D005260:Female; D006801:Humans; D008099:Liver / Q000098:blood supply / Q000473:pathology; D008113:Liver Neoplasms / Q000473:pathology* / Q000601:surgery; D016031:Liver Transplantation; D016015:Logistic Models; D008297:Male; D055806:Microvessels / Q000473:pathology*; D008875:Middle Aged; D008954:Models, Biological; D009361:Neoplasm Invasiveness; D016571:Neural Networks, Computer; D010865:Pilot Projects; D011237:Predictive Value of Tests; D011300:Preoperative Care / Q000379:methods* / Q000592:standards; D011379:Prognosis; D012372:ROC Curve; D012008:Recurrence; D012720:Severity of Illness Index