title: A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection.
abstract: We present a de novo design of an objective for use in multi-photon (MPM) and second harmonic generation (SHG) microscopy. This objective was designed to have a large field of view (FOV), while maintaining a moderate numerical aperture (NA) and relative straight forward construction. A dichroic beam splitter was incorporated within the objective itself allowing for an increase in the front aperture of the objective and corresponding enhancement of the solid angle of collected emission by an order of magnitude over existing designs.
authors.affiliation: School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA.
authors.forename: Jesse D
authors.initials: JD
authors.lastname: McMullen
authors.forename: Warren R
authors.initials: WR
authors.lastname: Zipfel
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*
references.citation: Nat Biotechnol. 2003 Nov;21(11):1369-77
references.pmid: 14595365
references.citation: Opt Express. 2009 Apr 13;17(8):6421-35
references.pmid: 19365467
references.citation: J Opt Soc Am A Opt Image Sci Vis. 2006 Dec;23(12):3139-49
references.pmid: 17106469
references.citation: J Microsc. 2007 Dec;228(Pt 3):330-7
references.pmid: 18045327
references.citation: Appl Opt. 2002 Sep 1;41(25):5376-82
references.pmid: 12211567
references.citation: Opt Express. 2007 Aug 6;15(16):10154-62
references.pmid: 19547364
references.citation: Opt Lett. 2006 Aug 15;31(16):2447-9
references.pmid: 16880851
references.citation: Opt Express. 2005 Jul 11;13(14):5528-34
references.pmid: 19498549
references.citation: Clin Gastroenterol Hepatol. 2008 Jan;6(1):95-101
references.pmid: 18065276
references.citation: Science. 1990 Apr 6;248(4951):73-6
references.pmid: 2321027
references.citation: Proc SPIE Int Soc Opt Eng. 2009;7161:
references.pmid: 19360140
references.citation: Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7075-80
references.pmid: 12756303
references.citation: Opt Lett. 2004 Nov 1;29(21):2521-3
references.pmid: 15584281