Description of Holtermanniella gen. nov., including Holtermanniella takashimae sp. nov. and four new combinations, and proposal of the order Holtermanniales to accommodate tremellomycetous yeasts of the Holtermannia clade.
The novel genus Holtermanniella is proposed here to accommodate four Cryptococcus species closely related to Holtermannia corniformis that are included in the Holtermannia clade (Basidiomycota, Agaricomycotina). Thus, four novel combinations are proposed: Holtermanniella nyarrowii comb. nov., Holtermanniella festucosa comb. nov., Holtermanniella mycelialis comb. nov. and Holtermanniella wattica comb. nov. In addition, a novel anamorphic yeast species was studied with 15 isolates obtained from different habitats around the world. Analysis of the sequences of the D1/D2 region of their large subunit rDNA showed that the novel species is placed phylogenetically within the Holtermannia clade of the Tremellomycetes (Agaricomycotina, Basidiomycota). PCR fingerprinting and sequencing of ITS1-5.8S-ITS2 showed genetic intraspecific variability among the strains: three groups were formed, which did not correlate with geographical origin or substrate. This novel species, designated the type species of Holtermanniella gen. nov., is described as Holtermanniella takashimae sp. nov.; the type strain is CBS 11174(T) (=HB 982(T) =DBVPG 8012(T)). The order Holtermanniales ord. nov. is proposed here to include Holtermannia (the type genus) and Holtermanniella.
Wuczkowski
Michael
M
University of Natural Resources and Applied Life Sciences, Institute of Applied Microbiology (IAM), Austrian Center of Biological Resources and Applied Mycology (ACBR), Muthgasse 18, 1190 Wien, Austria.
Passoth
Volkmar
V
Swedish University of Agricultural Sciences (SLU), Department for Microbiology, Uppsala, Sweden.
Turchetti
Benedetta
B
Department of Applied Biology & Industrial Yeasts Collection DBVPG, Università di Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy.
Andersson
Ann-Christin
AC
Swedish University of Agricultural Sciences (SLU), Department for Microbiology, Uppsala, Sweden.
Olstorpe
Matilda
M
Swedish University of Agricultural Sciences (SLU), Department for Microbiology, Uppsala, Sweden.
Laitila
Arja
A
VTT Technical Research Centre of Finland, PO Box 1000, Tietotie 2, Espoo, 02044 VTT, Finland.
Theelen
Bart
B
CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
van Broock
María
M
Lab. Microbiología Aplicada y Biotecnología, INIBIOMA, CONICET-UNComahue, Quintral 1250, (8400), Bariloche, Río Negro, Argentina.
Buzzini
Pietro
P
Department of Applied Biology & Industrial Yeasts Collection DBVPG, Università di Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy.
Prillinger
Hansjörg
H
University of Natural Resources and Applied Life Sciences, Institute of Applied Microbiology (IAM), Austrian Center of Biological Resources and Applied Mycology (ACBR), Muthgasse 18, 1190 Wien, Austria.
Sterflinger
Katja
K
University of Natural Resources and Applied Life Sciences, Institute of Applied Microbiology (IAM), Austrian Center of Biological Resources and Applied Mycology (ACBR), Muthgasse 18, 1190 Wien, Austria.
Schnürer
Johan
J
Swedish University of Agricultural Sciences (SLU), Department for Microbiology, Uppsala, Sweden.
Boekhout
Teun
T
CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Libkind
Diego
D
Lab. Microbiología Aplicada y Biotecnología, INIBIOMA, CONICET-UNComahue, Quintral 1250, (8400), Bariloche, Río Negro, Argentina.
D001487:Basidiomycota / Q000145:classification* / Q000235:genetics; D016000:Cluster Analysis; D003454:Cryptococcus / Q000145:classification* / Q000235:genetics; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S; C065096:RNA, ribosomal, 26S