title: The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B.
abstract: This study aimed to identify the inflammation-associated 7/4-antigen, which is highly expressed on neutrophils, inflammatory monocytes, some activated macrophages, as well as on bone marrow myeloid-restricted progenitors. The high expression on inflammatory cells is suggestive of a role in inflammation and makes the 7/4-antigen a potential target for the manipulation of inflammatory cells. Consistent with this, the 7/4-antibody mediates specific depletion of 7/4-expressing neutrophils and monocytes. We have identified the 7/4-antigen as a 25- to 30-kDa GPI-anchored glycoprotein synonymous with the Ly-6B.2 alloantigen. We characterized the expression of Ly-6B during the inflammatory reaction induced by zymosan. During the later stages of an experimental, acute, self-resolving inflammatory response, we found that Ly-6B is differentially expressed on macrophages. Ly-6B-expressing macrophages also express more MHCII, CIITA, CCR2, Ly-6C, and CD62L than the Ly-6B-negative macrophages, which in turn, express more of the resident tissue macrophage marker SIGN-R1 and higher CD11b and F4/80. Ly-6B-expressing macrophages incorporate more BrdU than their Ly-6B-negative contemporaries when fed during the resolution phase of the acute inflammatory response. Thus, Ly-6B expression on mature macrophages defines a subset of recently generated inflammatory macrophages that retain monocytic markers and is hence a surrogate marker of macrophage turnover in inflammatory lesions. The definition of the 7/4:Ly-6B antigen will allow further characterization and specific modulation of Ly-6B-expressing cells in vivo.
authors.affiliation: Infection, Immunity and Biochemistry, Cardiff University School of Medicine, Cardiff, UK.
authors.forename: Marcela
authors.initials: M
authors.lastname: Rosas
authors.forename: Benjamin
authors.initials: B
authors.lastname: Thomas
authors.forename: Martin
authors.initials: M
authors.lastname: Stacey
authors.forename: Siamon
authors.initials: S
authors.lastname: Gordon
authors.forename: Philip R
authors.initials: PR
authors.lastname: Taylor
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D002874:Chromosome Mapping; D008040:Genetic Linkage; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D041681:NIH 3T3 Cells; D010538:Peritonitis / Q000276:immunology; D010641:Phenotype
chemical_list: D000950:Antigens, Ly; C526827:Ly6 protein, mouse
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