title: ELISA measurement for urinary 3-hydroxyproline-containing peptides and its preliminary application to healthy persons and cancer patients.
abstract: OBJECTIVE
  We reported that endogenous urinary 3-hydroxyproline (3-Hyp) is useful for cancer screening because cancer invasion involves the destruction of basement membrane. A simple and sensitive assay is desired.
  
  
  METHODS
  An ELISA method using a specific antibody against a synthetic peptide of 10 amino acids including 3-Hyp corresponding to the amino acid sequences of collagen type IV alpha chain was applied to urine samples from 180 healthy controls and 22 cancer patients.
  
  
  RESULTS
  The values in controls were 2.44+/-1.90 (SD) mg peptide/g creatinine for 52 men and 2.87+/-2.01 for 128 women, while the values in 22 cancer patients were very low at 0.110+/-0.137 (p<0.001).
  
  
  CONCLUSIONS
  The discrepancy in the data between our previous and present studies is based on the difference of targets measured. 3-Hyp-containing peptides in cancer patients might be destroyed by the elevated peptidase levels found in these patients.
  
  
  CONCLUSIONS
  This ELISA assay may be useful for cancer screening.
authors.affiliation: Department of Internal Medicine, Sanno Hospital, International University of Health and Welfare, Minato-Ku, Tokyo 107-0052, Japan. iokazaki@iuhw.ac.jp.
authors.forename: Junichi
authors.initials: J
authors.lastname: Saito
authors.forename: Yasutada
authors.initials: Y
authors.lastname: Imamura
authors.forename: Joji
authors.initials: J
authors.lastname: Itoh
authors.forename: Shigeo
authors.initials: S
authors.lastname: Matsuyama
authors.forename: Akie
authors.initials: A
authors.lastname: Maruta
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Hayashi
authors.forename: Ataru
authors.initials: A
authors.lastname: Sato
authors.forename: Norihito
authors.initials: N
authors.lastname: Wada
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kashiwazaki
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Inagaki
authors.forename: Tetsu
authors.initials: T
authors.lastname: Watanabe
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kitagawa
authors.forename: Isao
authors.initials: I
authors.lastname: Okazaki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000652:urine*; D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000652:urine*; D008297:Male; D008403:Mass Screening; D008875:Middle Aged; D009369:Neoplasms / Q000652:urine*; D010190:Pancreatic Neoplasms / Q000652:urine; D010455:Peptides / Q000652:urine*; D013274:Stomach Neoplasms / Q000652:urine
chemical_list: D000906:Antibodies; D014408:Biomarkers, Tumor; D010455:Peptides; D006909:Hydroxyproline