Variation of physiochemical properties and cell association activity of membrane vesicles with growth phase in Pseudomonas aeruginosa.
Pseudomonas aeruginosa and other Gram-negative bacteria release membrane vesicles (MVs) from their surfaces, and MVs have an ability to interact with bacterial cells. Although it has been known that many bacteria have mechanisms that control their phenotypes with the transition from exponential phase to stationary phase, changes of properties in released MVs have been poorly understood. Here, we demonstrate that MVs released by P. aeruginosa during the exponential and stationary phases possess different physiochemical properties. MVs purified from the stationary phase had higher buoyant densities than did those purified from the exponential phase. Surface charge, characterized by zeta potential, of MVs tended to be more negative as the growth shifted to the stationary phase, although the charges of PAO1 cells were not altered. Pseudomonas quinolone signal (PQS), one of the regulators related to MV production in P. aeruginosa, was lower in MVs purified from the exponential phase than in those from the stationary phase. MVs from the stationary phase more strongly associated with P. aeruginosa cells than did those from the exponential phase. Our findings suggest that properties of MVs are altered to readily interact with bacterial cells along with the growth transition in P. aeruginosa.
Tashiro
Yosuke
Y
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Ichikawa
Sosaku
S
Shimizu
Motoyuki
M
Toyofuku
Masanori
M
Takaya
Naoki
N
Nakajima-Kambe
Toshiaki
T
Uchiyama
Hiroo
H
Nomura
Nobuhiko
N
D002499:Centrifugation, Density Gradient; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D015363:Quinolones / Q000032:analysis; D022142:Secretory Vesicles / Q000737:chemistry* / Q000378:metabolism*; D055672:Static Electricity
C407944:2-heptyl-3-hydroxy-4-quinolone; D015363:Quinolones
BMC Microbiol. 2009 Feb 03;9:26
19192306
Microbes Environ. 2010;25(1):1-7
21576845
Anal Biochem. 1976 May 7;72:248-54
942051
J Electron Microsc (Tokyo). 2003;52(5):465-9
14700078
Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11229-34
10500159
Mol Microbiol. 2008 Jul;69(2):491-502
18630345
J Bacteriol. 2003 Apr;185(7):2080-95
12644477
J Bacteriol. 1998 May;180(9):2306-11
9573179
J Bacteriol. 1996 May;178(10):2767-74
8631663
Mol Microbiol. 2006 Dec;62(6):1689-99
17083468
Genes Cells. 1999 Mar;4(3):135-43
10320479
J Bacteriol. 2009 Jul;191(13):4097-102
19429627
Microbiol Rev. 1979 Mar;43(1):73-102
111024
J Bacteriol. 1998 Oct;180(20):5478-83
9765585
J Antimicrob Chemother. 2006 Apr;57(4):764-6
16446373
J Bacteriol. 2008 Dec;190(24):7947-56
18931133
J Bacteriol. 2006 Aug;188(16):5945-57
16885463
Microbes Infect. 2006 Aug;8(9-10):2400-8
16807039
Mol Microbiol. 2007 Jul;65(1):153-65
17581126
Mol Microbiol. 2003 Oct;50(1):29-43
14507361
Appl Environ Microbiol. 2001 Feb;67(2):491-4
11157207
J Bacteriol. 1995 Dec;177(24):7155-63
8522523
Anal Biochem. 1980 May 1;104(1):10-4
6892980
J Bacteriol. 1995 Jul;177(14):3998-4008
7608073
Microbiology (Reading). 2003 May;149(Pt 5):1311-1322
12724392
Infect Immun. 2008 Oct;76(10):4624-32
18663005
Antimicrob Agents Chemother. 2004 Apr;48(4):1320-8
15047536
Microbiology (Reading). 2003 Oct;149(Pt 10):2789-2795
14523112
FEMS Microbiol Lett. 2010 Mar;304(2):123-30
20146747
Nature. 2005 Sep 15;437(7057):422-5
16163359
Mol Microbiol. 2006 Aug;61(4):839-46
16879642
Proc Natl Acad Sci U S A. 2004 Feb 3;101(5):1339-44
14739337
J Bacteriol. 2007 May;189(10):3776-83
17351035
J Bacteriol. 1996 May;178(9):2479-88
8626312
J Bacteriol. 2009 Dec;191(24):7509-19
19837799
Microbiology (Reading). 2004 Jul;150(Pt 7):2161-2169
15256559
Mol Microbiol. 2006 Sep;61(5):1308-21
16879411
J Microbiol Methods. 2001 Jan;43(3):153-64
11118650
J Bacteriol. 2008 Jun;190(11):3969-78
18390657