title: Oxidative stress is independently associated with non-alcoholic fatty liver disease (NAFLD) in subjects with and without type 2 diabetes.
abstract: OBJECTIVE
  Our work is aimed at exploring the interrelationship of oxidative stress and insulin resistance in NAFLD subjects with and without type 2 diabetes in a population-based study.
  
  
  METHODS
  Subjects [n=200] were recruited from the Chennai Urban Rural Epidemiology Study. 1: Normal glucose tolerance (NGT) subjects without NAFLD; 2: NGT with NAFLD; 3: type 2 diabetic subjects [T2DM] without NAFLD and 4: T2DM with NAFLD. Thiobarbituric acid reactive substances (TBARS), protein carbonyl (PCC) and glutathione levels were measured by standard methods. Ultrasound of the liver was used to diagnose NAFLD.
  
  
  RESULTS
  TBARS and PCC levels were significantly elevated and GSH/GSSG ratio was significantly decreased in diabetic subjects with NAFLD compared to all other groups (p trend <0.001). Oxidative stress markers significantly associated with NAFLD even after adjusting for age, gender, BMI and glycemic status.
  
  
  CONCLUSIONS
  Increased oxidative stress is independently associated with NAFLD in Asian Indians without and with T2DM.
authors.affiliation: Madras Diabetes Research Foundation, Gopalapuram, Chennai, India.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Narasimhan
authors.forename: Kuppan
authors.initials: K
authors.lastname: Gokulakrishnan
authors.forename: Rangasamy
authors.initials: R
authors.lastname: Sampathkumar
authors.forename: Syed
authors.initials: S
authors.lastname: Farooq
authors.forename: Radhakrishnan
authors.initials: R
authors.lastname: Ravikumar
authors.forename: Viswanathan
authors.initials: V
authors.lastname: Mohan
authors.forename: Muthuswamy
authors.initials: M
authors.lastname: Balasubramanyam
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications; D005234:Fatty Liver / Q000097:blood* / Q000150:complications; D005978:Glutathione / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008875:Middle Aged; D018384:Oxidative Stress; D050050:Protein Carbonylation; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D015415:Biomarkers; D007328:Insulin; D017392:Thiobarbituric Acid Reactive Substances; D005978:Glutathione