Immunoprecipitation to examine protein complexes.
Immunoprecipitation approaches are widely used in the field of cell biology. This method is ideally suited to probe interactions between proteins that make up highly complex organelles like eukaryotic cilia and flagella. In this chapter, I discuss general methods for carrying out and analyzing immunoprecipitation experiments and discuss the controls that should be included in all valid immunoprecipitation experiments.
Pazour
Gregory J
GJ
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000737:chemistry / Q000378:metabolism; D001681:Biological Assay / Q000379:methods / Q000592:standards; D047468:Immunoprecipitation / Q000379:methods*; D046912:Multiprotein Complexes / Q000737:chemistry*; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
D000936:Antigen-Antibody Complex; D046912:Multiprotein Complexes; D011506:Proteins