[Immune polymorphism analysis of HLA class II antigens in ulcer diseases associated with Helicobacter pylori in children].
Previous investigations have linked specific HLA class II alleles DRB1 and DQB1 to H. pylori infection (Y. Huang et al., 2005).


OBJECTIVE
to investigate potential contribution of HLA-DRB1 and DQB1 alleles in H. pylori infection susceptibility in a Russian pediatric population.


METHODS
Polymerase chain reaction-sequence specific primer (PCR-SSP) method was used to study the HLA-DRB1, DQB1 allelic frequency distribution in 162 children (93 female) with H. pylori infection was determined by culture, breath test and histology.


RESULTS
The carrier frequency of DQB1*03 was higher among H. pylori--positive patients with chronic gastritis only compared with H. pylori-negative patients. The difference in carrier frequencies for HLA-DRB1*17 was higher in H. pylori-positive ulcer patients compared with an uninfected controls (chi2 = 3.69, p = 0.027). In addition, the frequency of genotypes that possess HLA-DQB1*07 allele in the H. pylori-positive children (with peptic ulcer/ chronic gastritis only) was significantly lower than that in the H. pylori-negative control group.


CONCLUSIONS
HLA-DQB1*07 allele may be associated with protection against H. pylori infection independently of clinical outcome. At the same time, HLA-DRB1*17 allele might be associated with susceptible gene to peptic ulcer formation among H. pylori-positive children.
Nizhevich
A A
AA
Shcherbakov
P L
PL
Akhmadeeva
E N
EN
Sataev
V U
VU
Elicheva
Z M
ZM
Usmanova
I Z
IZ
Tsyglintseva
N P
NP
D000293:Adolescent; D000483:Alleles; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D005260:Female; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics* / Q000276:immunology; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D059811:HLA-DRB1 Chains; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D010437:Peptic Ulcer / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D011110:Polymorphism, Genetic
D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains