Disturbed tooth germ development in the absence of MINT in the cultured mouse mandibular explants.
The Msx2-interacting nuclear target protein (MINT) is a nuclear matrix protein that regulates the development of many tissues. However, little is known regarding the role of MINT in tooth development. In this study, we prepared polyclonal antibodies against MINT, and found that that MINT was expressed in different cells at each stage of tooth germ development by immunohistochemistry. The role of MINT in tooth development was further illustrated by the misshapen and severely hypoplastic tooth organ in the cultured mandibular explants of MINT deficient mice. From the initiation to cap stage, the differences between mutants and wild-type molars were more and more distinguished histologically. In the MINT-deficient mandibular explants, the tooth germ was reduced in the overall size and lacked enamel knot, with abnormal dental lamina and collapsed stellate reticulum. Furthermore, the BrdU incorporation experiment showed that the proliferation activity was significantly reduced in MINT-deficient dental epithelium. Our results suggest that MINT plays an important role in tooth development, in particular, epithelial morphogenesis.
Zhu
Ming-Hui
MH
Department of General and Emergency Dentistry, College of Stomatology, Xian, China.
Dong
Wen-Bo
WB
Dong
Guang-Ying
GY
Zhang
Ping
P
Chen
Yong-Jin
YJ
Wu
Bu-Ling
BL
Han
Hua
H
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D049109:Cell Proliferation; D004268:DNA-Binding Proteins; D004847:Epithelial Cells / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D008334:Mandible / Q000166:cytology*; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D018345:Mice, Knockout; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D009924:Organ Culture Techniques; D016601:RNA-Binding Proteins; D014070:Tooth / Q000196:embryology* / Q000378:metabolism; D014083:Tooth Germ / Q000166:cytology* / Q000196:embryology*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C121253:Spen protein, mouse; D001973:Bromodeoxyuridine
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