Rapid characterization of high/low producer CHO cells using matrix-assisted laser desorption/ionization time-of-flight.
An intact-cell mass spectrometry (ICM) method using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) was evaluated for the screening of stable recombinant Chinese hamster ovary (CHO) cell lines, an important mammalian cell line in bioprocessing. With rapid and simple cell pretreatments, viabilities of cells could be rapidly distinguished on the different fingerprints of mass spectra. Detectable m/z values on cell surfaces and their relative intensities were processed by two biostatistical methods, principle components analysis (PCA) and partial least squares (PLS), with promising results. Discrimination among cell lines with different expressed recombinant proteins or different productivities could be achieved. The ICM method has the advantage of providing multiple parameters simultaneously and possesses the potential to become a powerful method for routine monitoring of bioprocesses.
Feng
Hua-Tao
HT
Bioprocessing Technology Institute, A*STAR (Agency for Science, Technology and Research), 20 Biopolis Way, Centros, Singapore 138668. feng_huatao@bti.a-star.edu.sg
Wong
Niki S C
NS
Sim
Lyn Chiin
LC
Wati
Lydia
L
Ho
Yingswan
Y
Lee
May May
MM
D000818:Animals; D016466:CHO Cells / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D002470:Cell Survival; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D016018:Least-Squares Analysis; D025341:Principal Component Analysis; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D011994:Recombinant Proteins; D007371:Interferon-gamma