Enhancement of bone formation ex vivo and in vivo by a helioxanthin-derivative.
To effectively treat serious bone defects using bone-regenerative medicine, a small chemical compound that potently induces bone formation must be developed. We previously reported on the osteogenic effect of 4-(4-methoxyphenyl)pyrido[40,30:4,5]thieno[2,3-b]pyridine-2-carboxamide (TH), a helioxanthin-derivative, in vitro. Here, we report on TH's osteogenic effects ex vivo and in vivo. TH-induced new bone formation in both calvarial and metatarsal organ cultures. A novel monitoring system of osteoblastic differentiation using MC3T3-E1 cells revealed that TH was released from alpha-TCP bone cement and this release continued for more than one month. Lastly, the implantation of the alpha-TCP carrier containing TH into defects in mouse skull resulted in increased new bone areas within the defects after 4 weeks. A TH-containing scaffold may help establish a more efficient bone regeneration system.
Nakajima
Keiji
K
Division of Sensory and Motor System Medicine, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Komiyama
Yusuke
Y
Hojo
Hironori
H
Ohba
Shinsuke
S
Yano
Fumiko
F
Nishikawa
Naoko
N
Ihara
Sigeo
S
Aburatani
Hiroyuki
H
Takato
Tsuyoshi
T
Chung
Ung-Il
UI
D016475:3T3 Cells; D000818:Animals; D001861:Bone Regeneration; D017705:Lignans / Q000494:pharmacology*; D008684:Metatarsus; D051379:Mice; D009924:Organ Culture Techniques; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D011725:Pyridines / Q000494:pharmacology*; D012886:Skull; D058924:Thienopyridines; D013876:Thiophenes / Q000494:pharmacology*
C550145:4-(4-methoxyphenyl)pyrido(4',3'-4,5)thieno(2,3-b)pyridine-2-carboxamide; D017705:Lignans; D011725:Pyridines; D058924:Thienopyridines; D013876:Thiophenes; C505802:helioxanthin