The effects of estrogen, progesterone and polypropylene mesh on vaginal smooth muscle cell proliferation.
OBJECTIVE
To measure the effects of estrogen, progesterone and polypropylene mesh on vaginal smooth muscle cell proliferation.


METHODS
Primary smooth muscle cell (SMC) cultures were performed from vaginal biopsies which were then incubated with estradiol (0.1 microM, 1 microM, 10 microM), progesterone (5 nM, 50 nM, 0.5 microM), or polypropylene mesh to assess cell proliferation.


RESULTS
In vitro vaginal SMC proliferation was significantly increased by estradiol but not by progesterone or by polypropylene mesh (relative cell number, mean +/- SD, control vs. estrogen 0.24 +/- 0.02 vs. 0.28 +/- 0.02, P=0.01; control vs. progesterone 0.24 +/- 0.02 vs. 0.23 +/- 0.02, P=NS and control vs. mesh, 0.24 +/- 0.02 vs. 0.24 +/- 0.01, P=NS). In addition, estradiol increased cell proliferation in a dose responsive fashion (estradiol dose: 0.1 microM, 1 microM, 10 microM) compared to control (P=0.01).


CONCLUSIONS
Vaginal SMC proliferation is significantly increased by estrogen.
Takacs
Peter
P
Department of Obstetrics and Gynecology, University of Miami Miller School of Medicine, Miami, FL 33010, USA. ptakacs@med.miami.edu
Zhang
Yanping
Y
Jaramillo
Sindy
S
Bardawil
Tarek
T
Candiotti
Keith
K
Medina
Carlos A
CA
D049109:Cell Proliferation; D002478:Cells, Cultured; D004967:Estrogens / Q000502:physiology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D011126:Polypropylenes; D011374:Progesterone / Q000502:physiology*; D013526:Surgical Mesh; D014621:Vagina / Q000166:cytology / Q000502:physiology
D004967:Estrogens; D011126:Polypropylenes; D011374:Progesterone