title: Disruption of the non-canonical WNT pathway in lung squamous cell carcinoma.
abstract: Disruptions of beta-catenin and the canonical Wnt pathway are well documented in cancer. However, little is known of the non-canonical branch of the Wnt pathway. In this study, we investigate the transcript level patterns of genes in the Wnt pathway in squamous cell lung cancer using reverse-transcriptase (RT)-PCR. It was found that over half of the samples examined exhibited dysregulated gene expression of multiple components of the non-canonical branch of the WNT pathway. In the cases where beta catenin (CTNNB1) was not over-expressed, we identified strong relationships of expression between wingless-type MMTV integration site family member 5A (WNT5A)/ frizzled homolog 2 (FZD2), frizzled homolog 3 (FZD3) / dishevelled 2 (DVL2), and low density lipoprotein receptor-related protein 5 (LRP5)/ secreted frizzled-related protein 4 (SFRP4). This is one of the first studies to demonstrate expression of genes in the non-canonical pathway in normal lung tissue and its disruption in lung squamous cell carcinoma. These findings suggest that the non-canonical pathway may have a more prominent role in lung cancer than previously reported.
authors.affiliation: Department of Cancer Genetics and Developmental Biology, BC Cancer Agency Research Centre, Vancouver, BC, Canada.
authors.forename: Eric H L
authors.initials: EH
authors.lastname: Lee
authors.forename: Raj
authors.initials: R
authors.lastname: Chari
authors.forename: Andy
authors.initials: A
authors.lastname: Lam
authors.forename: Raymond T
authors.initials: RT
authors.lastname: Ng
authors.forename: John
authors.initials: J
authors.lastname: Yee
authors.forename: John
authors.initials: J
authors.lastname: English
authors.forename: Kenneth G
authors.initials: KG
authors.lastname: Evans
authors.forename: Calum
authors.initials: C
authors.lastname: Macaulay
authors.forename: Stephen
authors.initials: S
authors.lastname: Lam
authors.forename: Wan L
authors.initials: WL
authors.lastname: Lam
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