The effect of a combination of 0.1% octenidine dihydrochloride and 2% 2-phenoxyethanol (octenisept) on wound healing in pigs in vivo and its in vitro percutaneous permeation through intact and barrier disrupted porcine skin.
A combination of 0.1% octenidine dihydrochloride and 2% 2-phenoxyethanol (octenisept) is a commonly used disinfectant in human medicine. As porcine skin represents an adequate model for human skin, the effect of octenidine dihydrochloride and phenoxyethanol on wound healing is studied in pigs. Furthermore, the in vitro percutaneous permeation of the test substances is studied. The impact of the test formulations on wound healing is examined (A) under non occlusive conditions and (B) in comparison to another disinfectant based on povidone-iodine under occlusive conditions, while wounds are treated daily with the test substances. The percutaneous permeation of octenidine dihydrochloride and phenoxyethanol is studied in Franz-type diffusion cells with intact skin as well as barrier disrupted after tape stripping. Compared with povidone-iodine or vehicle treatment as well as untreated control wounds the treatment of wounds with the test formulation has no influence on the healing rate in pigs and does not induce retardation of wound healing. The in vitro diffusion experiment reveals that octenidine dihydrochloride is only detectable in the acceptor chamber of three-barrier disrupted skin samples. Phenoxyethanol permeates through intact porcine skin in amounts of 11.3% and through barrier disrupted skin in amounts of 43.9%
Stahl
Jessica
J
Department of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany. jessica.stahl@tiho-hannover.de
Braun
Michael
M
Siebert
Joerg
J
Kietzmann
Manfred
M
D000287:Administration, Topical; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004338:Drug Combinations; D005026:Ethylene Glycols / Q000008:administration & dosage* / Q000493:pharmacokinetics; D007097:Imines; D066298:In Vitro Techniques; D008297:Male; D017137:Nonoxynol / Q000008:administration & dosage; D009779:Occlusive Dressings; D010539:Permeability; D011206:Povidone-Iodine / Q000008:administration & dosage; D011725:Pyridines / Q000008:administration & dosage* / Q000493:pharmacokinetics; D034421:Sus scrofa; D014945:Wound Healing / Q000187:drug effects*
D000891:Anti-Infective Agents, Local; D004338:Drug Combinations; D005026:Ethylene Glycols; D007097:Imines; D011725:Pyridines; D017137:Nonoxynol; D011206:Povidone-Iodine; C005398:phenoxyethanol; C034213:octenidine
J Periodontol. 1987 Sep;58(9):628-33
3477629
Food Chem Toxicol. 1997 Oct-Nov;35(10-11):1009-16
9463535
J Antimicrob Chemother. 2007 Jun;59(6):1280-1
17439976
S Afr J Surg. 1992 Jun;30(2):40-1
1519121
Int J Infect Dis. 2008 Nov;12(6):e19-25
18538605
Dermatology. 2009;218(1):67-8
18987463
Scand J Dent Res. 1981 Oct;89(5):384-92
6952538
Antimicrob Agents Chemother. 1985 Dec;28(6):786-90
3909955
J Antimicrob Chemother. 1990 Feb;25(2):237-45
2184158
Infect Control Hosp Epidemiol. 2005 Aug;26(8):703-7
16156327
Clin Microbiol Rev. 1999 Jan;12(1):147-79
9880479
Pharm Res. 2006 Aug;23(8):1850-6
16841197
Altern Lab Anim. 2008 May;36(2):201-13
18522486
J Periodontal Res. 1986 Mar;21(2):154-62
2937903
Dermatology. 2006;212 Suppl 1:91-3
16490982
Bioorg Med Chem. 2007 May 15;15(10):3422-9
17383187
J Med Chem. 1984 Nov;27(11):1457-64
6492075
Altern Lab Anim. 2006 Oct;34(5):499-513
17121474
Fundam Appl Toxicol. 1987 Feb;8(2):272-9
3556838
Zentralbl Hyg Umweltmed. 1989 May;188(1-2):188-93
2757739
J Pharm Pharmacol. 1992 Aug;44(8):640-5
1359086
J Tissue Viability. 2006 May;16(2):20-3
16752710
FEBS Lett. 1971 Jul 15;16(1):63-67
11945902
J Hosp Infect. 2002 Aug;51(4):305-7
12183146
Wound Repair Regen. 2001 Mar-Apr;9(2):66-76
11350644
Plast Reconstr Surg. 2001 Sep 1;108(3):675-87
11698839
J Surg Res. 1983 Aug;35(2):142-8
6887836
Infection. 2002 Oct;30(5):282-5
12382087
Acta Dermatovenerol Alp Pannonica Adriat. 2008 Sep;17(3):139-43
18853079
Curr Probl Dermatol. 1978;7:39-52
752456
J Invest Dermatol. 2005 Aug;125(2):264-9
16098036
J Antimicrob Chemother. 2008 Jun;61(6):1281-7
18364400
Skin Pharmacol Physiol. 2004 May-Jun;17(3):141-6
15087593
J Wound Care. 1995 Sep;4(8):366-7; discussion 368-71
7553187