Simultaneous expression of Arabidopsis ρ-hydroxyphenylpyruvate dioxygenase and MPBQ methyltransferase in transgenic corn kernels triples the tocopherol content.
The quantity and composition of tocopherols (compounds with vitamin E activity) vary widely among different plant species reflecting the expression, activity and substrate specificity of enzymes in the corresponding metabolic pathway. Two Arabidopsis cDNA clones corresponding to ρ-hydroxyphenylpyruvate dioxygenase (HPPD) and 2-methyl-6-phytylplastoquinol methyltransferase (MPBQ MT) were constitutively expressed in corn to further characterize the pathway and increase the kernel tocopherol content. Transgenic kernels contained up to 3 times as much γ-tocopherol as their wild type counterparts whereas other tocopherol isomers remained undetectable. Biofortification by metabolic engineering offers a sustainable alternative to vitamin E supplementation for the improvement of human health.
Naqvi
Shaista
S
Departament de Producció Vegetal i Ciència Forestal, Universitat de Lleida, 25198, Lleida, Spain.
Farré
Gemma
G
Zhu
Changfu
C
Sandmann
Gerhard
G
Capell
Teresa
T
Christou
Paul
P
D015111:4-Hydroxyphenylpyruvate Dioxygenase / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering / Q000379:methods; D006801:Humans; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D015854:Up-Regulation; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics; D024504:gamma-Tocopherol / Q000378:metabolism*
D024504:gamma-Tocopherol; D015111:4-Hydroxyphenylpyruvate Dioxygenase; C447422:2-methyl-6-phytylbenzoquinone methyltransferase; D008780:Methyltransferases
Arch Biochem Biophys. 1985 Apr;238(1):290-9
3985624
Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18232-7
19011084
Proc Natl Acad Sci U S A. 2009 May 12;106(19):7762-7
19416835
FEBS Lett. 2003 Apr 10;540(1-3):35-40
12681479
Trends Plant Sci. 2007 Dec;12(12):548-55
18006362
Transgenic Res. 1996 May;5(3):213-8
8673150
FASEB J. 2001 Nov;15(13):2314-25
11689457
Annu Rev Nutr. 1996;16:321-47
8839930
Am J Clin Nutr. 1998 Apr;67(4):669-84
9537614
Transgenic Res. 2010 Apr;19(2):165-80
19685153
Plant Cell. 2003 Dec;15(12):3007-19
14630966
Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:133-161
15012206
Science. 1998 Dec 11;282(5396):2098-100
9851934