Optimized speckle variance OCT imaging of microvasculature.
We optimize speckle variance optical coherence tomography (svOCT) imaging of microvasculature in high and low bulk tissue motion scenarios. To achieve a significant level of image contrast, frame rates must be optimized such that tissue displacement between frames is less than the beam radius. We demonstrate that higher accuracy estimates of speckle variance can enhance the detection of capillaries. These findings are illustrated in vivo by imaging the dorsal window chamber model (low bulk motion). We also show svOCT imaging of the nonstabilized finger (high bulk motion), using optimized imaging parameters, demonstrating better vessel detection than Doppler OCT.
Mariampillai
Adrian
A
Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.
Leung
Michael K K
MK
Jarvi
Mark
M
Standish
Beau A
BA
Lee
Kenneth
K
Wilson
Brian C
BC
Vitkin
Alex
A
Yang
Victor X D
VX
D018316:Gliosarcoma / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D055806:Microvessels / Q000166:cytology* / Q000473:pathology / Q000502:physiology / Q000503:physiopathology; D009068:Movement; D009262:Nails / Q000098:blood supply; D019047:Phantoms, Imaging; D041623:Tomography, Optical Coherence / Q000379:methods*