Age-related changes in bone structure and strength in female and male BALB/c mice.
Mice may be useful for studies of skeletal aging, but there are limited data on changes in bone structure and strength over their life span. We obtained bones from female and male BALB/c mice at ages 2, 4, 7, 12, and 20 months and evaluated their structural, densitometric, and mechanical properties. MicroCT of the mid-diaphysis of the femur and radius indicated that during skeletal growth (2-7 months) bone cross-sectional size (area, moment of inertia) increased rapidly; during aging (7-20 months) cortical area was maintained, while moment of inertia continued to increase. Bones from females were smaller than those from males at young ages but not at later ages. Changes in whole-bone stiffness and strength reflected the changes in bone size, with a rapid increase from 2 to 7 months, followed by little or no change. In contrast, energy-to-fracture declined with aging. Cortical tissue mineral density increased during growth and was maintained with aging. MicroCT of trabecular bone revealed age-related changes that were site-dependent. The proximal tibia showed a clear pattern of age-related decline in trabecular BV/TV, with progressive decreases after 4 months in both sexes; lumbar vertebra L5 had more modest age-related declines; in contrast, caudal vertebra Ca7 had increasing BV/TV with aging. Overall, we found no evidence that females had more pronounced age-related deterioration than males. We conclude that bones from aging female and male BALB/c mice exhibit many of the changes seen in humans and are therefore a clinically relevant model for studies of skeletal aging.
Willinghamm
Mark D
MD
Department of Orthopedic Surgery, Washington University School of Medicine, 1 Barnes-Jewish Hospital Plaza, St. Louis, MO 63110, USA.
Brodt
Michael D
MD
Lee
Kristen L
KL
Stephens
Abby L
AL
Ye
Jiaxin
J
Silva
Matthew J
MJ
D000375:Aging / Q000473:pathology* / Q000502:physiology*; D000818:Animals; D015519:Bone Density; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000000981:diagnostic imaging / Q000502:physiology*; D019245:Compressive Strength; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013314:Stress, Mechanical; D014057:Tomography, X-Ray Computed
J Biomech. 1996 Feb;29(2):257-60
8849821
J Orthop Res. 1995 May;13(3):309-16
7602391
J Bone Miner Res. 1999 Aug;14(8):1394-403
10457272
J Bone Miner Res. 2005 May;20(5):817-27
15824855
J Biomech. 1977;10(4):223-31
858728
J Bone Miner Res. 2008 Dec;23(12):1964-73
18665791
Bone. 1998 Jan;22(1):57-66
9437514
J Bone Joint Surg Am. 1995 Mar;77(3):387-95
7890787
J Bone Miner Res. 1996 May;11(5):568-77
9157771
J Bone Miner Res. 2002 Jun;17(6):1044-50
12054159
J Clin Endocrinol Metab. 1996 Jun;81(6):2264-70
8964862
J Bone Miner Res. 2002 Sep;17(9):1597-603
12211429
J Biomech. 2005 Mar;38(3):467-75
15652544
N Engl J Med. 2003 Jul 24;349(4):327-34
12878739
Bone. 1996 May;18(5):397-403
8739896
J Bone Miner Res. 2005 Nov;20(11):1983-91
16234972
Bone. 2002 Jul;31(1):1-7
12110404
J Bone Joint Surg Am. 1976 Jan;58(1):82-6
1249116
J Bone Miner Res. 2007 Aug;22(8):1197-207
17488199
J Bone Joint Surg Am. 1993 Aug;75(8):1193-205
8354678
J Bone Miner Res. 2008 Dec;23(12):2048-59
18684086
Bone. 1987;8(2):79-85
3593611
Bone. 2004 Aug;35(2):425-31
15268893
J Orthop Res. 1988;6(6):886-96
3171769
J Bone Miner Res. 2006 Jan;21(1):78-88
16355276
J Bone Miner Res. 2006 Dec;21(12):1856-63
17002580
J Bone Miner Res. 1999 Dec;14(12):2159-66
10620076
J Bone Miner Res. 2004 Dec;19(12):1945-54
15537436
Bone. 2003 Sep;33(3):387-98
13678781
J Bone Joint Surg Am. 1997 Mar;79(3):421-7
9070533
Calcif Tissue Int. 1994 Feb;54(2):113-8
8012866