[Immunoregulatory function of Radix Glycyrrhizae polysaccharide in tumor-bearing mice].
OBJECTIVE
To observe the effects of Radix Glycyrrhizae polysaccharide on regulatory T cells (Treg) in spleen and lymphocyte transformation ratio in tumor-bearing mice so as to explore the mechanisms of its immunoregulatory function.


METHODS
Fifty BALB/c mice were randomly divided into normal group, untreated group, cyclophosphamide group, Radix Glycyrrhizae polysaccharide group and Radix Glycyrrhizae polysaccharide plus cyclophosphamide group. Except normal group, mice were subcutaneously implanted H22 tumor cells in the right axillary region. After 24 h, mice in normal and untreated group were subcutaneously injected with physiological saline, while mice in the cyclophosphamide group were intraperitoneally injected with cyclophosphamide and mice in Radix Glycyrrhizae polysaccharide group were subcutaneously injected with polysaccharide. Fourteen days later, Treg cells of spleen were detected by flow cytometry and lymphocyte transformation ratio was detected by methyl thiazolyl tetrazolium method.


RESULTS
The proportion of Treg cells was significantly higher in the untreated group than in the normal group, and was lower in the Radix Glycyrrhizae polysaccharide group than in the untreated group (P < p0.01). Lymphocyte transformation ratio in the Radix Glycyrrhizae polysaccharide group was higher than that in the cyclophosphamide group. There was no interaction between Radix Glycyrrhizae polysaccharide and cyclophosphamide.


CONCLUSIONS
Radix Glycyrrhizae polysaccharide can regulate the cellular immunity disorders of tumor-bearing mice by decreasing proportion of Treg cells and increasing spleen lymphocyte transformation ratio.
Li
Xiao-bing
XB
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
He
Xiao-juan
XJ
Liu
Biao
B
Xu
Li
L
Ju
Da-hong
DH
Jiang
Miao
M
Lu
Ai-ping
AP
D000818:Animals; D005434:Flow Cytometry; D006035:Glycyrrhiza / Q000737:chemistry*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D011134:Polysaccharides / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D011134:Polysaccharides