title: Enhancement of bone formation ex vivo and in vivo by a helioxanthin-derivative.
abstract: To effectively treat serious bone defects using bone-regenerative medicine, a small chemical compound that potently induces bone formation must be developed. We previously reported on the osteogenic effect of 4-(4-methoxyphenyl)pyrido[40,30:4,5]thieno[2,3-b]pyridine-2-carboxamide (TH), a helioxanthin-derivative, in vitro. Here, we report on TH's osteogenic effects ex vivo and in vivo. TH-induced new bone formation in both calvarial and metatarsal organ cultures. A novel monitoring system of osteoblastic differentiation using MC3T3-E1 cells revealed that TH was released from alpha-TCP bone cement and this release continued for more than one month. Lastly, the implantation of the alpha-TCP carrier containing TH into defects in mouse skull resulted in increased new bone areas within the defects after 4 weeks. A TH-containing scaffold may help establish a more efficient bone regeneration system.
authors.affiliation: Division of Sensory and Motor System Medicine, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Nakajima
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Komiyama
authors.forename: Hironori
authors.initials: H
authors.lastname: Hojo
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ohba
authors.forename: Fumiko
authors.initials: F
authors.lastname: Yano
authors.forename: Naoko
authors.initials: N
authors.lastname: Nishikawa
authors.forename: Sigeo
authors.initials: S
authors.lastname: Ihara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Aburatani
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takato
authors.forename: Ung-Il
authors.initials: UI
authors.lastname: Chung
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001861:Bone Regeneration; D017705:Lignans / Q000494:pharmacology*; D008684:Metatarsus; D051379:Mice; D009924:Organ Culture Techniques; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D011725:Pyridines / Q000494:pharmacology*; D012886:Skull; D058924:Thienopyridines; D013876:Thiophenes / Q000494:pharmacology*
chemical_list: C550145:4-(4-methoxyphenyl)pyrido(4',3'-4,5)thieno(2,3-b)pyridine-2-carboxamide; D017705:Lignans; D011725:Pyridines; D058924:Thienopyridines; D013876:Thiophenes; C505802:helioxanthin