title: An RNA-hydrolyzing recombinant antibody exhibits an antiviral activity against classical swine fever virus.
abstract: Some proteins with ribonuclease (RNase) activity have been shown to suppress viral replication. A well-characterized recombinant antibody, 3D8 single-chain variable fragment (3D8 scFv), has RNA-hydrolyzing and cell-penetrating activities. Here, we investigated antiviral activity of 3D8 scFv against classical swine fever virus (CSFV). In a cell line expressing 3D8 scFv (C26), intracellular RNA-hydrolysis activity was higher compared to control PK-15 cells and viral replication was strongly suppressed at the viral RNA level, with the evidence of independency of IFN-alpha/beta induction. Exogenous treatment of 3D8 scFv, prior to or post-CSFV infection, was also shown to suppress CSFV replication at the viral RNA level. These observations suggest that antiviral activity of 3D8 scFv may be due to the intrinsic RNase activity of 3D8 scFv, which is capable of targeting viral RNA genomes or transcripts.
authors.affiliation: Department of Microbiology, Ajou University School of Medicine, San 5, Wonchon-dong, Yeongtong-gu, Suwon 443-721, Republic of Korea.
authors.forename: Hye-Ryeong
authors.initials: HR
authors.lastname: Jun
authors.forename: Chuong D
authors.initials: CD
authors.lastname: Pham
authors.forename: Seong-In
authors.initials: SI
authors.lastname: Lim
authors.forename: Suk-Chan
authors.initials: SC
authors.lastname: Lee
authors.forename: Yong-Sung
authors.initials: YS
authors.lastname: Kim
authors.forename: Sun
authors.initials: S
authors.lastname: Park
authors.forename: Myung-Hee
authors.initials: MH
authors.lastname: Kwon
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000378:metabolism / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000378:metabolism / Q000494:pharmacology*; D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D006691:Classical Swine Fever / Q000276:immunology / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000187:drug effects*; D015536:Down-Regulation; D006868:Hydrolysis; D007370:Interferon Type I / Q000276:immunology; D012367:RNA, Viral / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology*; D012260:Ribonucleases / Q000378:metabolism / Q000494:pharmacology; D057127:Single-Chain Antibodies / Q000276:immunology / Q000494:pharmacology*; D013552:Swine; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D007370:Interferon Type I; D012367:RNA, Viral; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; D012260:Ribonucleases