title: Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion.
abstract: Amassments of heterochromatin in somatic cells occur in close contact with the nuclear envelope (NE) but are gapped by channel- and cone-like zones that appear largely free of heterochromatin and associated with the nuclear pore complexes (NPCs). To identify proteins involved in forming such heterochromatin exclusion zones (HEZs), we used a cell culture model in which chromatin condensation induced by poliovirus (PV) infection revealed HEZs resembling those in normal tissue cells. HEZ occurrence depended on the NPC-associated protein Tpr and its large coiled coil-forming domain. RNAi-mediated loss of Tpr allowed condensing chromatin to occur all along the NE's nuclear surface, resulting in HEZs no longer being established and NPCs covered by heterochromatin. These results assign a central function to Tpr as a determinant of perinuclear organization, with a direct role in forming a morphologically distinct nuclear sub-compartment and delimiting heterochromatin distribution.
authors.affiliation: Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Krull
authors.forename: Julia
authors.initials: J
authors.lastname: Dörries
authors.forename: Björn
authors.initials: B
authors.lastname: Boysen
authors.forename: Sonja
authors.initials: S
authors.lastname: Reidenbach
authors.forename: Lars
authors.initials: L
authors.lastname: Magnius
authors.forename: Helene
authors.initials: H
authors.lastname: Norder
authors.forename: Johan
authors.initials: J
authors.lastname: Thyberg
authors.forename: Volker C
authors.initials: VC
authors.lastname: Cordes
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D020868:Gene Silencing; D006367:HeLa Cells; D006570:Heterochromatin / Q000737:chemistry*; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry*; D017955:Poliovirus / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000737:chemistry*; D034622:RNA Interference
chemical_list: D002843:Chromatin; D006570:Heterochromatin; D028861:Nuclear Pore Complex Proteins; D011518:Proto-Oncogene Proteins; C073597:TPR protein, human
references.citation: J Cell Sci. 1997 Apr;110 ( Pt 8):927-44
references.pmid: 9152019
references.citation: Virology. 1997 Dec 8;239(1):176-85
references.pmid: 9426457
references.citation: J Ultrastruct Res. 1968 Dec;25(5):452-500
references.pmid: 5714586
references.citation: Genes Cells. 2002 Apr;7(4):421-34
references.pmid: 11952838
references.citation: Exp Cell Res. 1991 Mar;193(1):72-7
references.pmid: 1995303
references.citation: Genes Dev. 2005 May 15;19(10):1188-98
references.pmid: 15905407
references.citation: Curr Opin Cell Biol. 2006 Jun;18(3):307-16
references.pmid: 16687245
references.citation: J Cell Sci. 1979 Feb;35:59-66
references.pmid: 422683
references.citation: EMBO J. 2001 Oct 15;20(20):5703-14
references.pmid: 11598013
references.citation: Mol Biol Cell. 2006 Feb;17(2):760-9
references.pmid: 16314393
references.citation: J Cell Sci. 2001 Feb;114(Pt 3):463-74
references.pmid: 11171316
references.citation: Science. 1981 Dec 18;214(4527):1353-5
references.pmid: 7313694
references.citation: Nat Rev Mol Cell Biol. 2007 Oct;8(10):761-73
references.pmid: 17786152
references.citation: J Cell Sci. 2000 Jul;113 ( Pt 14):2585-93
references.pmid: 10862716
references.citation: Trends Cell Biol. 2007 Mar;17(3):127-34
references.pmid: 17197184
references.citation: Exp Cell Res. 2005 Jan 15;302(2):180-93
references.pmid: 15561100
references.citation: J Virol. 2005 Apr;79(7):4460-9
references.pmid: 15767446
references.citation: J Biol Chem. 1991 Aug 5;266(22):14714-20
references.pmid: 1713590
references.citation: Cell. 1995 Apr 21;81(2):215-22
references.pmid: 7736573
references.citation: Histochem Cell Biol. 1996 Feb;105(2):153-61
references.pmid: 8852437
references.citation: J Cell Sci. 1974 Nov;16(2):261-99
references.pmid: 4141361
references.citation: Trends Biochem Sci. 2004 Apr;29(4):175-82
references.pmid: 15082311
references.citation: Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2450-5
references.pmid: 12604785
references.citation: J Cell Biol. 1998 Dec 28;143(7):1801-12
references.pmid: 9864356
references.citation: Results Probl Cell Differ. 2002;35:25-48
references.pmid: 11791407
references.citation: Mol Cell Biol. 2006 Nov;26(21):7858-70
references.pmid: 16954382
references.citation: J Cell Sci. 2008 Apr 1;121(Pt 7):1119-27
references.pmid: 18334550
references.citation: J Virol. 2004 Sep;78(18):10166-77
references.pmid: 15331749
references.citation: J Struct Biol. 2004 Mar;145(3):272-88
references.pmid: 14960378
references.citation: Proc Natl Acad Sci U S A. 2006 May 16;103(20):7688-93
references.pmid: 16682630
references.citation: Nature. 2001 May 24;411(6836):494-8
references.pmid: 11373684
references.citation: Mol Cell Biol. 1996 Aug;16(8):4163-71
references.pmid: 8754815
references.citation: J Cell Biol. 1997 Jul 14;138(1):131-42
references.pmid: 9214387
references.citation: J Struct Biol. 1997 Aug;119(3):284-94
references.pmid: 9245768
references.citation: Mol Biol Cell. 2002 Apr;13(4):1282-97
references.pmid: 11950939
references.citation: Biophys J. 2008 Jul;95(2):877-85
references.pmid: 18375513
references.citation: Mol Biol Cell. 2003 Feb;14(2):600-10
references.pmid: 12589057
references.citation: J Biophys Biochem Cytol. 1961 Mar;9:671-87
references.pmid: 13720098
references.citation: J Submicrosc Cytol. 1985 Oct;17(4):687-94
references.pmid: 3001333
references.citation: Cell Biol Int Rep. 1988 Sep;12(9):669-89
references.pmid: 3058320
references.citation: J Biol Chem. 2009 Dec 11;284(50):34911-7
references.pmid: 19840948
references.citation: J Cell Biol. 1995 Mar;128(5):721-36
references.pmid: 7876300
references.citation: J Comp Neurol. 1989 Dec 15;290(3):440-50
references.pmid: 2592622
references.citation: J Cell Biol. 2001 Jul 9;154(1):21-4
references.pmid: 11448986
references.citation: Mol Biol Cell. 2003 May;14(5):1923-40
references.pmid: 12802065
references.citation: Cell. 1993 Jan 15;72(1):29-38
references.pmid: 8422679
references.citation: J Cell Biol. 1970 Oct;47(1):211-21
references.pmid: 4105038
references.citation: Genes Dev. 2008 Nov 1;22(21):2926-31
references.pmid: 18981471
references.citation: Bioessays. 2005 May;27(5):477-87
references.pmid: 15832379
references.citation: Nature. 2002 Jul 25;418(6896):430-4
references.pmid: 12087357
references.citation: Histochem Cell Biol. 2005 Mar;123(3):217-28
references.pmid: 15830242
references.citation: Mol Biol Cell. 2005 Dec;16(12):5610-20
references.pmid: 16195343
references.citation: Cold Spring Harb Symp Quant Biol. 1993;58:777-92
references.pmid: 7525149
references.citation: J Cell Biol. 2009 Mar 9;184(5):647-57
references.pmid: 19273613
references.citation: J Virol. 1992 May;66(5):3062-8
references.pmid: 1313918
references.citation: Biochim Biophys Acta. 2008 Nov;1783(11):2100-7
references.pmid: 18723053
references.citation: J Cell Biol. 2002 Feb 18;156(4):617-30
references.pmid: 11839768
references.citation: Cell. 1981 Dec;27(3 Pt 2):555-61
references.pmid: 6086108
references.citation: EMBO J. 2009 Dec 16;28(24):3785-98
references.pmid: 19927119
references.citation: J Biophys Biochem Cytol. 1959 Oct;6:147-56
references.pmid: 13843146
references.citation: Mol Biol Cell. 2004 Sep;15(9):4261-77
references.pmid: 15229283
references.citation: J Struct Biol. 2002 Oct-Dec;140(1-3):254-67
references.pmid: 12490173
references.citation: Mol Biol Cell. 2004 Apr;15(4):1991-2002
references.pmid: 14718558
references.citation: Mol Cell. 2006 Mar 17;21(6):811-23
references.pmid: 16543150
references.citation: J Biol Chem. 2006 Jan 13;281(2):1296-304
references.pmid: 16286466
references.citation: J Cell Biol. 1998 Apr 6;141(1):31-49
references.pmid: 9531546
references.citation: J Struct Biol. 1993 Sep-Oct;111(2):148-57
references.pmid: 8130038
references.citation: J Biol Chem. 2007 Feb 2;282(5):3042-9
references.pmid: 17158105
references.citation: J Cell Sci. 2000 Jan;113 Pt 2:291-302
references.pmid: 10633080
references.citation: J Biol Chem. 2007 Nov 23;282(47):33902-7
references.pmid: 17897941
references.citation: J Cell Sci. 2005 Dec 15;118(Pt 24):5825-34
references.pmid: 16317046
references.citation: Curr Opin Genet Dev. 2007 Apr;17(2):100-6
references.pmid: 17317147
references.citation: J Virol. 2008 Feb;82(4):1647-55
references.pmid: 18045934
references.citation: Nat Rev Genet. 2007 Feb;8(2):104-15
references.pmid: 17230197
references.citation: J Cell Biol. 1997 Feb 10;136(3):515-29
references.pmid: 9024684
references.citation: Muscle Nerve. 2001 Jun;24(6):826-9
references.pmid: 11360268
references.citation: Mol Biol Cell. 2009 Apr;20(8):2146-59
references.pmid: 19225157
references.citation: Exp Cell Res. 1970 May;60(2):175-84
references.pmid: 5463771
references.citation: J Cell Biol. 1976 Sep;70(3):714-9
references.pmid: 182701
references.citation: Brookhaven Symp Biol. 1959 Nov;12:134-52
references.pmid: 13836127
references.citation: Chromosome Res. 2003;11(5):387-401
references.pmid: 12971716
references.citation: J Virol. 1973 Apr;11(4):565-74
references.pmid: 4121707
references.citation: Mol Cell Biol. 2008 Nov;28(22):6954-66
references.pmid: 18794356
references.citation: J Cell Biol. 1971 Nov;51(21):558-63
references.pmid: 4939527
references.citation: J Cell Biol. 2001 Oct 29;155(3):339-54
references.pmid: 11684705
references.citation: Genes Dev. 2007 Dec 1;21(23):3027-43
references.pmid: 18056419
references.citation: Nat Struct Mol Biol. 2007 Nov;14(11):1049-55
references.pmid: 17984967
references.citation: Exp Cell Res. 1998 Nov 25;245(1):43-56
references.pmid: 9828100
references.citation: Nat Rev Mol Cell Biol. 2000 Nov;1(2):137-43
references.pmid: 11253366
references.citation: J Mol Biol. 1971 Sep 14;60(2):303-23
references.pmid: 4106575
references.citation: Mol Cell Biol. 1999 Aug;19(8):5768-84
references.pmid: 10409764
references.citation: Curr Biol. 1999 Mar 25;9(6):285-91
references.pmid: 10209094
references.citation: Chromosoma. 1998 Sep;107(4):228-36
references.pmid: 9745047
references.citation: EMBO Rep. 2003 Sep;4(9):861-6
references.pmid: 12947417
references.citation: J Cell Biol. 1970 Jan;44(1):218-23
references.pmid: 5409459
references.citation: Eur J Cell Biol. 1991 Feb;54(1):150-6
references.pmid: 2032545
references.citation: Science. 2004 Nov 19;306(5700):1387-90
references.pmid: 15514115
references.citation: EMBO J. 2005 Feb 23;24(4):813-23
references.pmid: 15692572
references.citation: EMBO J. 2001 Jan 15;20(1-2):240-9
references.pmid: 11226174
references.citation: Curr Opin Genet Dev. 2009 Apr;19(2):172-9
references.pmid: 19339170
references.citation: J Cell Biol. 1968 Dec;39(3):630-60
references.pmid: 5699935
references.citation: Chromosoma. 2002 Nov;111(4):236-55
references.pmid: 12424524
references.citation: Proc Natl Acad Sci U S A. 1975 Jul;72(7):2626-30
references.pmid: 241077
references.citation: Curr Opin Genet Dev. 2007 Oct;17(5):373-80
references.pmid: 17913491
references.citation: Cell. 2007 Feb 23;128(4):787-800
references.pmid: 17320514
references.citation: Mol Biol Cell. 2001 Aug;12(8):2433-52
references.pmid: 11514627
references.citation: BMC Cell Biol. 2009 Oct 16;10:74
references.pmid: 19835572
references.citation: Scanning. 1997 Aug;19(5):368-75
references.pmid: 9262022
references.citation: Chromosome Res. 1993 Jul;1(2):93-106
references.pmid: 8143096
references.citation: J Struct Biol. 1991 Dec;107(3):291-308
references.pmid: 1725493
references.citation: Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1010-5
references.pmid: 12531921
references.citation: Cell. 2004 Jan 9;116(1):63-73
references.pmid: 14718167
references.citation: J Mol Biol. 1996 Jul 19;260(3):304-11
references.pmid: 8757794
references.citation: Semin Cell Dev Biol. 2007 Oct;18(5):668-75
references.pmid: 17904880
references.citation: Oncogene. 1992 Nov;7(11):2329-33
references.pmid: 1437155
references.citation: J Cell Biol. 1966 Oct;31(1):55-77
references.pmid: 5971975
references.citation: Biol Cell. 2004 Oct;96(8):595-601
references.pmid: 15519694
references.citation: J Virol. 2006 Mar;80(6):2705-17
references.pmid: 16501080
references.citation: Int Rev Cytol Suppl. 1977;(6):75-186
references.pmid: 348630
references.citation: Methods. 2006 Jan;38(1):17-24
references.pmid: 16343937