Anti-clastogenic effect of berberine against DMBA-induced clastogenesis.
Our aim was to evaluate the protective effect of berberine on 7,12-dimethylbenz[a]anthracene (DMBA)-induced chromosomal aberrations and micro-nucleated polychromatic erythrocytes (MnPCEs) frequency in bone marrow cells of golden Syrian hamsters. The anti-clastogenic effect of berberine (50 mg/kg b.w. p.o.) was also assessed by measuring the status of phase II detoxification enzymes and oxidative stress, as biochemical endpoints, during DMBA (30 mg/kg b.w. i.p.) induced clastogenesis. Marked chromosomal aberrations, increased MnPCEs frequency and enhanced status of lipid peroxidation, antioxidants and phase I and II detoxification enzymes were noticed in hamsters treated with DMBA alone. Oral pre-treatment with berberine for 5 days to DMBA-treated hamsters significantly reduced the frequency of MnPCEs and chromosomal abnormalities as well as reversed the status of lipid peroxidation, antioxidants and phase I and II detoxification enzymes. The present study thus suggests that berberine has potent anti-clastogenic potential against DMBA-induced clastogenesis, which is probably due to its anti-lipid peroxidative potential and effect on modulation of phase I and II detoxification cascade.
Sindhu
Ganapathy
G
Department of Biochemistry & Biotechnology, Faculty of Science, Annamalai University, Tamil Nadu, India.
Manoharan
Shanmugam
S
D015127:9,10-Dimethyl-1,2-benzanthracene; D000284:Administration, Oral; D000818:Animals; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001599:Berberine / Q000008:administration & dosage / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000648:ultrastructure; D002869:Chromosome Aberrations / Q000187:drug effects*; D020552:Comet Assay; D006224:Cricetinae; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008647:Mesocricetus; D015162:Micronucleus Tests; D018384:Oxidative Stress / Q000187:drug effects
D016587:Antimutagenic Agents; D000975:Antioxidants; D001599:Berberine; D015127:9,10-Dimethyl-1,2-benzanthracene