Mammalian DEAD box protein Ddx51 acts in 3' end maturation of 28S rRNA by promoting the release of U8 snoRNA.
Biogenesis of eukaryotic ribosomes requires a number of RNA helicases that drive molecular rearrangements at various points of the assembly pathway. While many ribosome synthesis factors are conserved among all eukaryotes, certain features of ribosome maturation, such as U8 snoRNA-assisted processing of the 5.8S and 28S rRNA precursors, are observed only in metazoan cells. Here, we identify the mammalian DEAD box helicase family member Ddx51 as a novel ribosome synthesis factor and an interacting partner of the nucleolar GTP-binding protein Nog1. Unlike any previously studied yeast helicases, Ddx51 is required for the formation of the 3' end of 28S rRNA. Ddx51 binds to pre-60S subunit complexes and promotes displacement of U8 snoRNA from pre-rRNA, which is necessary for the removal of the 3' external transcribed spacer from 28S rRNA and productive downstream processing. These data demonstrate the emergence of a novel factor that facilitates a pre-rRNA processing event specific for higher eukaryotes.
Srivastava
Leena
L
Department of Cell Biology, University of Medicine and Dentistry of New Jersey, 2 Medical Center Drive, Stratford, NJ 08084, USA.
Lapik
Yevgeniya R
YR
Wang
Minshi
M
Pestov
Dimitri G
DG
D000818:Animals; D020029:Base Pairing / Q000235:genetics; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism; D055785:Gene Knockdown Techniques; D005799:Genes, Dominant / Q000235:genetics; D051379:Mice; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding; D026721:RNA 3' End Processing; D012322:RNA Precursors / Q000378:metabolism; D012339:RNA, Ribosomal, 28S / Q000378:metabolism*; D020537:RNA, Small Nucleolar / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D050505:Mutant Proteins; D009687:Nuclear Proteins; D012322:RNA Precursors; D012339:RNA, Ribosomal, 28S; D020537:RNA, Small Nucleolar; C550081:Ddx51 protein, mouse; D020558:GTP Phosphohydrolases; C524465:Nog1 protein, mouse; D053487:DEAD-box RNA Helicases
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