Methyl jasmonate- or gibberellins A3-induced astaxanthin accumulation is associated with up-regulation of transcription of beta-carotene ketolase genes (bkts) in microalga Haematococcus pluvialis.
The microalga Haematococcus pluvialis accumulates astaxanthin in response to abiotic stresses. Since methyl jasmonate (MJ) and gibberellins A(3) (GA(3)) are involved in the stress responses of plants, the impact of these compounds on astaxanthin metabolism was studied. Alga cells treated separately with MJ and GA(3) accumulated more astaxanthin than the controls. MJ and GA(3) treatment increased the transcription of three beta-carotene ketolase genes (bkts). MJ- and GA(3)-responsive cis-acting elements were identified in the 5'-flanking regions of bkt genes. These results suggest that MJ and GA(3) constitute molecular signals in the network of astaxanthin accumulation. Induction of astaxanthin accumulation by MJ or GA(3) without any other stimuli presents an attractive application potential.
Lu
Yandu
Y
YanTai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China.
Jiang
Peng
P
Liu
Shaofang
S
Gan
Qinhua
Q
Cui
Hongli
H
Qin
Song
S
D000085:Acetates / Q000378:metabolism*; D001483:Base Sequence; D003517:Cyclopentanes / Q000378:metabolism*; D017931:DNA Primers; D018076:DNA, Complementary; D056890:Eukaryota / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005875:Gibberellins / Q000378:metabolism*; D010105:Oxygenases / Q000235:genetics*; D054883:Oxylipins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D015854:Up-Regulation; D024341:Xanthophylls / Q000378:metabolism
D000085:Acetates; D003517:Cyclopentanes; D017931:DNA Primers; D018076:DNA, Complementary; D005875:Gibberellins; D054883:Oxylipins; D024341:Xanthophylls; C005948:astaxanthine; C072239:methyl jasmonate; C007842:gibberellic acid; D010105:Oxygenases; C093259:beta-carotene ketolase