Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion.
Amassments of heterochromatin in somatic cells occur in close contact with the nuclear envelope (NE) but are gapped by channel- and cone-like zones that appear largely free of heterochromatin and associated with the nuclear pore complexes (NPCs). To identify proteins involved in forming such heterochromatin exclusion zones (HEZs), we used a cell culture model in which chromatin condensation induced by poliovirus (PV) infection revealed HEZs resembling those in normal tissue cells. HEZ occurrence depended on the NPC-associated protein Tpr and its large coiled coil-forming domain. RNAi-mediated loss of Tpr allowed condensing chromatin to occur all along the NE's nuclear surface, resulting in HEZs no longer being established and NPCs covered by heterochromatin. These results assign a central function to Tpr as a determinant of perinuclear organization, with a direct role in forming a morphologically distinct nuclear sub-compartment and delimiting heterochromatin distribution.
Krull
Sandra
S
Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Germany.
Dörries
Julia
J
Boysen
Björn
B
Reidenbach
Sonja
S
Magnius
Lars
L
Norder
Helene
H
Thyberg
Johan
J
Cordes
Volker C
VC
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D020868:Gene Silencing; D006367:HeLa Cells; D006570:Heterochromatin / Q000737:chemistry*; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry*; D017955:Poliovirus / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000737:chemistry*; D034622:RNA Interference
D002843:Chromatin; D006570:Heterochromatin; D028861:Nuclear Pore Complex Proteins; D011518:Proto-Oncogene Proteins; C073597:TPR protein, human
J Cell Sci. 1997 Apr;110 ( Pt 8):927-44
9152019
Virology. 1997 Dec 8;239(1):176-85
9426457
J Ultrastruct Res. 1968 Dec;25(5):452-500
5714586
Genes Cells. 2002 Apr;7(4):421-34
11952838
Exp Cell Res. 1991 Mar;193(1):72-7
1995303
Genes Dev. 2005 May 15;19(10):1188-98
15905407
Curr Opin Cell Biol. 2006 Jun;18(3):307-16
16687245
J Cell Sci. 1979 Feb;35:59-66
422683
EMBO J. 2001 Oct 15;20(20):5703-14
11598013
Mol Biol Cell. 2006 Feb;17(2):760-9
16314393
J Cell Sci. 2001 Feb;114(Pt 3):463-74
11171316
Science. 1981 Dec 18;214(4527):1353-5
7313694
Nat Rev Mol Cell Biol. 2007 Oct;8(10):761-73
17786152
J Cell Sci. 2000 Jul;113 ( Pt 14):2585-93
10862716
Trends Cell Biol. 2007 Mar;17(3):127-34
17197184
Exp Cell Res. 2005 Jan 15;302(2):180-93
15561100
J Virol. 2005 Apr;79(7):4460-9
15767446
J Biol Chem. 1991 Aug 5;266(22):14714-20
1713590
Cell. 1995 Apr 21;81(2):215-22
7736573
Histochem Cell Biol. 1996 Feb;105(2):153-61
8852437
J Cell Sci. 1974 Nov;16(2):261-99
4141361
Trends Biochem Sci. 2004 Apr;29(4):175-82
15082311
Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2450-5
12604785
J Cell Biol. 1998 Dec 28;143(7):1801-12
9864356
Results Probl Cell Differ. 2002;35:25-48
11791407
Mol Cell Biol. 2006 Nov;26(21):7858-70
16954382
J Cell Sci. 2008 Apr 1;121(Pt 7):1119-27
18334550
J Virol. 2004 Sep;78(18):10166-77
15331749
J Struct Biol. 2004 Mar;145(3):272-88
14960378
Proc Natl Acad Sci U S A. 2006 May 16;103(20):7688-93
16682630
Nature. 2001 May 24;411(6836):494-8
11373684
Mol Cell Biol. 1996 Aug;16(8):4163-71
8754815
J Cell Biol. 1997 Jul 14;138(1):131-42
9214387
J Struct Biol. 1997 Aug;119(3):284-94
9245768
Mol Biol Cell. 2002 Apr;13(4):1282-97
11950939
Biophys J. 2008 Jul;95(2):877-85
18375513
Mol Biol Cell. 2003 Feb;14(2):600-10
12589057
J Biophys Biochem Cytol. 1961 Mar;9:671-87
13720098
J Submicrosc Cytol. 1985 Oct;17(4):687-94
3001333
Cell Biol Int Rep. 1988 Sep;12(9):669-89
3058320
J Biol Chem. 2009 Dec 11;284(50):34911-7
19840948
J Cell Biol. 1995 Mar;128(5):721-36
7876300
J Comp Neurol. 1989 Dec 15;290(3):440-50
2592622
J Cell Biol. 2001 Jul 9;154(1):21-4
11448986
Mol Biol Cell. 2003 May;14(5):1923-40
12802065
Cell. 1993 Jan 15;72(1):29-38
8422679
J Cell Biol. 1970 Oct;47(1):211-21
4105038
Genes Dev. 2008 Nov 1;22(21):2926-31
18981471
Bioessays. 2005 May;27(5):477-87
15832379
Nature. 2002 Jul 25;418(6896):430-4
12087357
Histochem Cell Biol. 2005 Mar;123(3):217-28
15830242
Mol Biol Cell. 2005 Dec;16(12):5610-20
16195343
Cold Spring Harb Symp Quant Biol. 1993;58:777-92
7525149
J Cell Biol. 2009 Mar 9;184(5):647-57
19273613
J Virol. 1992 May;66(5):3062-8
1313918
Biochim Biophys Acta. 2008 Nov;1783(11):2100-7
18723053
J Cell Biol. 2002 Feb 18;156(4):617-30
11839768
Cell. 1981 Dec;27(3 Pt 2):555-61
6086108
EMBO J. 2009 Dec 16;28(24):3785-98
19927119
J Biophys Biochem Cytol. 1959 Oct;6:147-56
13843146
Mol Biol Cell. 2004 Sep;15(9):4261-77
15229283
J Struct Biol. 2002 Oct-Dec;140(1-3):254-67
12490173
Mol Biol Cell. 2004 Apr;15(4):1991-2002
14718558
Mol Cell. 2006 Mar 17;21(6):811-23
16543150
J Biol Chem. 2006 Jan 13;281(2):1296-304
16286466
J Cell Biol. 1998 Apr 6;141(1):31-49
9531546
J Struct Biol. 1993 Sep-Oct;111(2):148-57
8130038
J Biol Chem. 2007 Feb 2;282(5):3042-9
17158105
J Cell Sci. 2000 Jan;113 Pt 2:291-302
10633080
J Biol Chem. 2007 Nov 23;282(47):33902-7
17897941
J Cell Sci. 2005 Dec 15;118(Pt 24):5825-34
16317046
Curr Opin Genet Dev. 2007 Apr;17(2):100-6
17317147
J Virol. 2008 Feb;82(4):1647-55
18045934
Nat Rev Genet. 2007 Feb;8(2):104-15
17230197
J Cell Biol. 1997 Feb 10;136(3):515-29
9024684
Muscle Nerve. 2001 Jun;24(6):826-9
11360268
Mol Biol Cell. 2009 Apr;20(8):2146-59
19225157
Exp Cell Res. 1970 May;60(2):175-84
5463771
J Cell Biol. 1976 Sep;70(3):714-9
182701
Brookhaven Symp Biol. 1959 Nov;12:134-52
13836127
Chromosome Res. 2003;11(5):387-401
12971716
J Virol. 1973 Apr;11(4):565-74
4121707
Mol Cell Biol. 2008 Nov;28(22):6954-66
18794356
J Cell Biol. 1971 Nov;51(21):558-63
4939527
J Cell Biol. 2001 Oct 29;155(3):339-54
11684705
Genes Dev. 2007 Dec 1;21(23):3027-43
18056419
Nat Struct Mol Biol. 2007 Nov;14(11):1049-55
17984967
Exp Cell Res. 1998 Nov 25;245(1):43-56
9828100
Nat Rev Mol Cell Biol. 2000 Nov;1(2):137-43
11253366
J Mol Biol. 1971 Sep 14;60(2):303-23
4106575
Mol Cell Biol. 1999 Aug;19(8):5768-84
10409764
Curr Biol. 1999 Mar 25;9(6):285-91
10209094
Chromosoma. 1998 Sep;107(4):228-36
9745047
EMBO Rep. 2003 Sep;4(9):861-6
12947417
J Cell Biol. 1970 Jan;44(1):218-23
5409459
Eur J Cell Biol. 1991 Feb;54(1):150-6
2032545
Science. 2004 Nov 19;306(5700):1387-90
15514115
EMBO J. 2005 Feb 23;24(4):813-23
15692572
EMBO J. 2001 Jan 15;20(1-2):240-9
11226174
Curr Opin Genet Dev. 2009 Apr;19(2):172-9
19339170
J Cell Biol. 1968 Dec;39(3):630-60
5699935
Chromosoma. 2002 Nov;111(4):236-55
12424524
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2626-30
241077
Curr Opin Genet Dev. 2007 Oct;17(5):373-80
17913491
Cell. 2007 Feb 23;128(4):787-800
17320514
Mol Biol Cell. 2001 Aug;12(8):2433-52
11514627
BMC Cell Biol. 2009 Oct 16;10:74
19835572
Scanning. 1997 Aug;19(5):368-75
9262022
Chromosome Res. 1993 Jul;1(2):93-106
8143096
J Struct Biol. 1991 Dec;107(3):291-308
1725493
Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1010-5
12531921
Cell. 2004 Jan 9;116(1):63-73
14718167
J Mol Biol. 1996 Jul 19;260(3):304-11
8757794
Semin Cell Dev Biol. 2007 Oct;18(5):668-75
17904880
Oncogene. 1992 Nov;7(11):2329-33
1437155
J Cell Biol. 1966 Oct;31(1):55-77
5971975
Biol Cell. 2004 Oct;96(8):595-601
15519694
J Virol. 2006 Mar;80(6):2705-17
16501080
Int Rev Cytol Suppl. 1977;(6):75-186
348630
Methods. 2006 Jan;38(1):17-24
16343937