Role of AP-1 and RE-1 binding sites in matrix metalloproteinase-2 transcriptional regulation in skeletal muscle atrophy.
Previous work has suggested that an extracellular matrix degrading enzyme-matrix metalloproteinase-2 (MMP-2) plays an important role in the development of muscle atrophy. However, the transcriptional regulation mechanism of MMP-2 in skeletal muscle atrophy remains largely unknown. Using transgenic MMP-2 promoter reporter mice, we have demonstrated that AP-1 and RE-1 binding sites in the MMP-2 promoter region, coupled with increased binding of Fra-1, Fra-2 and AP-2, play a critical role in MMP-2 transcriptional regulation in muscle atrophy. Novel information gained from this study has improved our understanding of in vivo transcriptional regulation of MMP-2 in skeletal muscle atrophy.
Liu
Xuhui
X
Department of Veterans Affairs, San Francisco Veterans Affairs Medical Center, 4150 Clement St, San Francisco, CA 94121, USA.
Manzano
Givenchy
G
Lovett
David H
DH
Kim
Hubert T
HT
D000818:Animals; D047369:Chromatin Immunoprecipitation; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000201:enzymology*; D009133:Muscular Atrophy / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014158:Transcription, Genetic; D000099229:RE1-Silencing Transcription Factor
D020778:Matrix Metalloproteinase 2; D012097:Repressor Proteins; D018808:Transcription Factor AP-1; D000099229:RE1-Silencing Transcription Factor