Small volume excitation and enhancement of dye fluorescence on a 2D photonic crystal surface.
We demonstrate an easy-to-implement scheme for fluorescence enhancement and observation volume reduction using photonic crystals (PhCs) as substrates for microscopy. By normal incidence coupling to slow 2D-PhC guided modes, a 65 fold enhancement in the excitation is achieved in the near field region (100 nm deep and 1 microm wide) of the resonant mode. Such large enhancement together with the high spatial resolution makes this device an excellent substrate for fluorescence microscopies.
Estrada
L C
LC
Quantum Electronics Lab, Dpto. de Física. FCEN, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina.
Martinez
O E
OE
Brunstein
M
M
Bouchoule
S
S
Le-Gratiet
L
L
Talneau
A
A
Sagnes
I
I
Monnier
P
P
Levenson
J A
JA
Yacomotti
A M
AM
D003287:Contrast Media / Q000032:analysis / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D007089:Image Enhancement / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D013499:Surface Properties
D003287:Contrast Media; D005456:Fluorescent Dyes