The compound isolated from the leaves of Phyllostachys nigra protects oxidative stress-induced retinal ganglion cells death.
This study was performed to determine whether the compound isolated from Phyllostachys nigra could attenuate oxidative stress in transformed retinal ganglion cells (RGC-5 cells) death. RGC-5 cells in culture were given two different insults such as l-buthionine-(S,R)-sulfoximine (BSO) plus glutamate for 24h or hydrogen peroxide for 24h, after which cell survival were measured. Among the four systematic fractions tested, ethyl acetate fraction showed a significantly higher inhibition which was in a concentration dependent manner. Eight compounds were isolated from ethyl acetate fraction of P. nigra using preparative RP-HPLC and Sephadex LH-20 column chromatography. Their chemical structures were elucidated by chemical and spectral analysis as isoorientin (1), orientin (2), vitexin (3), cis-coumaric acid (4), p-coumaric acid (5), luteolin 6-C-(6''-O-trans-caffeoylglucoside) (6), vittariflavone (7) and tricin (8). The luteolin 6-C-(6''-O-trans-caffeoylglucoside) (compound 6) significantly attenuated the negative effects of BSO plus glutamate or hydrogen peroxide to RGC-5 cells. Treatment of the RGC-5 cells with compound 6 reduced the reactive oxygen species (ROS) as quantified using 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA). This compound also replenished the reduced glutathione level. In conclusion, these results implicate that compound 6 isolated from P. nigra could be used as a leading compounds for retinal disease via anti-oxidative effects.
Lee
Hee Ju
HJ
Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Daejeon-dong, Gangneung, Republic of Korea.
Kim
Kyung-A
KA
Kang
Kui Dong
KD
Lee
Eun Ha
EH
Kim
Chul Young
CY
Um
Byung Hun
BH
Jung
Sang Hoon
SH
D031723:Bambusa / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D012160:Retina / Q000166:cytology / Q000187:drug effects*
D010936:Plant Extracts