Serum anti-PDIK1L autoantibody as a novel marker for endometriosis.
OBJECTIVE
To establish a novel serum marker for endometriosis, serum autoantibodies (autoAbs) were investigated using a proteomic approach.


METHODS
Retrospective study.


METHODS
Departments of Molecular Pathology and Obstetrics and Gynecology in Ehime University and University Hospital.


METHODS
Sixty-nine patients with endometriosis, 38 disease control patients without endometriosis, and 44 healthy volunteers.


METHODS
Autoantibodies in sera of endometriotic patients and healthy controls were investigated using a human fibroblast cell line, two-dimensional gel electrophoresis, and Western blotting. Proteins in reacted spots were identified using MALDI time of flight mass spectrometry with MASCOT analysis. ELISAs were established using recombinant proteins, and autoAb-titers were estimated in sera of endometriotic patients and controls.


METHODS
Identification of serum autoAb useful for diagnosis of endometriosis.


RESULTS
Several autoAbs were identified. ELISAs were established and serum autoAb titers were estimated. Among those identified, anti-PDIK1L-autoAb levels were significantly elevated in endometriotic patients. Sensitivity (59.4%) and accuracy (72.8%) of serum anti-PDIK1L-autoAb assay were better than those of serum CA125 levels (36.2% and 62.9%, respectively) in diagnosis of endometriosis. Additionally, anti-PDIK1L-autoAb could detect endometriotic patients in early stages.


CONCLUSIONS
Serum anti-PDIK1L-autoAb can be a new serum marker for the diagnosis of endometriosis. This study validates further clinical evaluation of this novel marker.
Nabeta
Motowo
M
Department of Molecular Pathology, Ehime University Graduate School of Medicine, Ehime, Japan.
Abe
Yasuhito
Y
Haraguchi
Ryuma
R
Kito
Katsumi
K
Kusanagi
Yasuki
Y
Ito
Masaharu
M
D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000032:analysis / Q000097:blood*; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D002277:Carcinoma / Q000097:blood / Q000276:immunology; D016022:Case-Control Studies; D002478:Cells, Cultured; D004715:Endometriosis / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000097:blood / Q000276:immunology; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood / Q000276:immunology; D017346:Protein Serine-Threonine Kinases / Q000276:immunology*; D012189:Retrospective Studies; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014591:Uterine Diseases / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D014594:Uterine Neoplasms / Q000097:blood / Q000276:immunology; D055815:Young Adult
D001323:Autoantibodies; D015415:Biomarkers; C482688:PDIK1L protein, human; D017346:Protein Serine-Threonine Kinases