Heat shock factor 1 is a transcription factor of Fas gene.
In mammalian cells, stress-induced expression of heat shock protein is controlled by heat shock factor 1 (HSF1). However, HSF1 functions as a regulator of additional genes. In this study, we observed that heat treatment effectively induced expression of Fas. Using bioinformatics, a high affinity and functional HSF1-binding element within the -1996/-1985 oligonucleotide of the 5'-flanking region of the Fas gene was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Exogenous expression of a constitutively activative HSF1, induced Fas gene transcription and protein synthesis in the absence of heat stress. Moreover, RNA interference-mediated HSF1 gene-silencing attenuated Fas expression in a heat-induced model. Our results suggested that HSF1 is an important transcription factor of Fas gene.
Shunmei
E
E
The Tradition Chinese Hospital of Guangdong Province, Guangzhou, 510120, China.
Zhao
Yuanbo
Y
Huang
Yunhong
Y
Lai
Kun
K
Chen
Cha
C
Zeng
Jianming
J
Zou
Jiangying
J
D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D019295:Computational Biology; D004247:DNA / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation / Q000235:genetics; D000076249:Heat Shock Transcription Factors; D006358:Hot Temperature; D006801:Humans; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes / Q000235:genetics; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D000076249:Heat Shock Transcription Factors; D050505:Mutant Proteins; D034741:RNA, Small Interfering; D014157:Transcription Factors; D019014:fas Receptor; D004247:DNA