Effect of dimethylsulphoxide and curcumin on protein carbonyls and reactive oxygen species of cerebral hemispheres of mice as a function of age.
Oxidative modification alters the function of proteins and is thought to play an important role in the decline of cellular functions during aging. Reactive oxygen species generated as by-products of oxidative metabolism frequently damage cellular macromolecules such as DNA, lipids and proteins. Protein carbonyls are commonly used as a marker of protein oxidation in cells and tissues. The brain is a highly oxidative organ consuming 20% of the body's oxygen despite accounting for only 2% of the total body weight. In the present study, we have evaluated the levels of free radicals and protein carbonyls in the cerebral hemispheres of young and old mice. Dimethylsulphoxide treatment to the mice has been shown to cause a reduction in reactive oxygen species and protein carbonylation. In this study, we have also investigated the combined effect of dimethylsulphoxide and curcumin on free radicals and protein oxidation. Curcumin (90mg/kg body weight) dissolved in dimethylsulphoxide was administered to the mice for three consecutive days. A significant reduction in the level of reactive oxygen species and protein carbonylation was seen with co-supplementation of the two antioxidants. The present study, thus demonstrated the antioxidative effects of dimethylsulphoxide and curcumin in the brain which may help in preventing free-radical-induced damage to the proteins during the later stages of life.
Dkhar
Preeticia
P
Department of Biochemistry, North Eastern Hill University, Shillong 793022, Meghalaya, India.
Sharma
Ramesh
R
D000375:Aging / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D003474:Curcumin / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004357:Drug Synergism; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D050050:Protein Carbonylation / Q000187:drug effects* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism*; D016896:Treatment Outcome
D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D003474:Curcumin; D004121:Dimethyl Sulfoxide