title: Quantitative Rose Bengal Test for diagnosis of bovine brucellosis.
abstract: The Rose Bengal Test (RBT) is the most widely used screening test for brucellosis in both humans and animals. Owing to its apparent simplicity of reading, however, interpretations of the RBT results can be affected by personal experience. This study describes a simple way to improve the accuracy and uniformity of reading the RBT reaction by counting the number of agglutinated particles using transparent OHP film with Quantity One, which was originally designed to count the bacterial colony numbers on agar plates. Using this system, the reactivities of three Rose Bengal antigens from different sources against international standard serum (1,000 units, VLA, UK) could be numerically measured: the intensity scale ranged from zero to around 1,600. This system enabled us to compare the antigenicity of Rose Bengal antigens from three different sources by using statistical analyses such as regression and mean intensity. Collectively, mathematical measuring of the reaction intensity used in this study may help interpret subtle test results by providing more reliable data and additional statistical information on the herd. In addition, the method would also be applicable to other agglutination test for other diseases.
authors.affiliation: Bacteriology and Parasitology Division, National Veterinary Research & Quarantine Service, Anyang, Republic of Korea. chodh@nvrqs.go.kr
authors.forename: Donghee
authors.initials: D
authors.lastname: Cho
authors.forename: Hyangmi
authors.initials: H
authors.lastname: Nam
authors.forename: Jongwan
authors.initials: J
authors.lastname: Kim
authors.forename: Eunjeong
authors.initials: E
authors.lastname: Heo
authors.forename: Yunsang
authors.initials: Y
authors.lastname: Cho
authors.forename: Inyeong
authors.initials: I
authors.lastname: Hwang
authors.forename: Jaemyung
authors.initials: J
authors.lastname: Kim
authors.forename: Jongman
authors.initials: J
authors.lastname: Kim
authors.forename: Sukchan
authors.initials: S
authors.lastname: Jung
authors.forename: Simon
authors.initials: S
authors.lastname: More
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000097:blood; D002006:Brucellosis / Q000175:diagnosis* / Q000276:immunology / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D015169:Colony Count, Microbial; D012015:Reference Standards; D012395:Rose Bengal
chemical_list: D000942:Antigens, Bacterial; D012395:Rose Bengal