title: Individual carboxypeptidase D domains have both redundant and unique functions in Drosophila development and behavior.
abstract: Metallocarboxypeptidase D (CPD) functions in protein and peptide processing. The Drosophila CPD svr gene undergoes alternative splicing, producing forms containing 1-3 active or inactive CP domains. To investigate the function of the various CP domains, we created transgenic flies expressing specific forms of CPD in the embryonic-lethal svr (PG33) mutant. All constructs containing an active CP domain rescued the lethality with varying degrees, and full viability required inactive CP domain-3. Transgenic flies overexpressing active CP domain-1 or -2 were similar to each other and to the viable svr mutants, with pointed wing shape, enhanced ethanol sensitivity, and decreased cold sensitivity. The transgenes fully compensated for a long-term memory deficit observed in the viable svr mutants. Overexpression of CP domain-1 or -2 reduced the levels of Lys/Arg-extended adipokinetic hormone intermediates. These findings suggest that CPD domains-1 and -2 have largely redundant functions in the processing of growth factors, hormones, and neuropeptides.
authors.affiliation: Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
authors.forename: Galyna
authors.initials: G
authors.lastname: Sidyelyeva
authors.forename: Christian
authors.initials: C
authors.lastname: Wegener
authors.forename: Brian P
authors.initials: BP
authors.lastname: Schoenfeld
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Bell
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Baker
authors.forename: Sean M J
authors.initials: SM
authors.lastname: McBride
authors.forename: Lloyd D
authors.initials: LD
authors.lastname: Fricker
mesh_terms: D017398:Alternative Splicing / Q000502:physiology; D000818:Animals; D030801:Animals, Genetically Modified; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D040461:Gene Components; D008568:Memory / Q000502:physiology; D010641:Phenotype; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology*; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014921:Wings, Animal / Q000033:anatomy & histology
chemical_list: D029721:Drosophila Proteins; D011506:Proteins; C094315:metallocarboxypeptidase D
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