Determination of polyamines in organic extracts from roots of Canavalia ensiformis by capillary electrophoresis.
A rapid, selective and specific capillary zone electrophoresis method to determine polyamines in organic extracts from roots of Canavalia ensiformis (Jack Beans) was developed using ultra violet (UV) detection. Canavalia ensiformis is relatively free from diseases and it is used as reference in allelopathy studies. Polyamines are widely distributed in plant and it could be involved in plant pathogen interactions. Optimal separation was achieved using 15 mmol L(-1)formic acid (pH 3.0) + 4 mmol L(-1) imidazole as a background electrolyte. It was possible to identify and quantify the polyamines on herbal samples in the presence of other phytochemical substances and analyze them quickly (up to 6 min). The applicability of this method was evaluated in crude organic extracts from roots of Canavalia ensiformis.
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Institute of Chemistry of São Carlos, University of São Paulo, São Carlos, São Paulo, Brazil.
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D032449:Canavalia / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary; D005561:Formates / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D011073:Polyamines / Q000032:analysis*; D013997:Time Factors
D004573:Electrolytes; D005561:Formates; D007093:Imidazoles; D009930:Organic Chemicals; D010936:Plant Extracts; D011073:Polyamines; C030544:formic acid; C029899:imidazole