title: Mouse oocyte vitrification: the effects of two methods on maturing germinal vesicle breakdown oocytes.
abstract: OBJECTIVE
  Evaluation of viability and subsequent developmental ability of mouse germinal vesicle breakdown oocytes vitrified in conventional straws.
  
  
  METHODS
  Oocytes with compact cumulus cells were cultured for 3 h in TCM199 medium GVBD and vitrified by two methods: the step-wise and single-step. After vitrification, the oocytes were thawed, and subjected to in vitro maturation and in vitro fertilization. Oocyte survival (post-thaw) was assessed by morphological appearance and staining, using propidium iodide (PI)/Hoechst 33342. The oocyte maturation and fertilization rates were examined in vitro.
  
  
  RESULTS
  In the single-step method the rates of post thaw survival, maturation to metaphase II and cleavage (2-cell embryos) were 58.68%, 56.41% and 38.63%, respectively. In the step-wise method, the corresponding rates were 81.75%, 68.59% and 51.80%, respectively.
  
  
  CONCLUSIONS
  Vitrification of mouse germinal vesicle breakdown oocytes by the step-wise method had the advantage of maintaining the viability and subsequent production of 2-cell embryos. In comparison with that in unvitrified control oocytes, the development of MII oocytes to 2-cell embryos was impaired following vitrification.
authors.affiliation: Department of Anat., Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Khosravi-Farsani
authors.forename: Aligholi
authors.initials: A
authors.lastname: Sobhani
authors.forename: Fardin
authors.initials: F
authors.lastname: Amidi
authors.forename: Reza
authors.initials: R
authors.lastname: Mahmoudi
mesh_terms: D000818:Animals; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D054885:Cumulus Cells / Q000166:cytology / Q000187:drug effects; D019855:Ethylene Glycol / Q000494:pharmacology*; D005260:Female; D005306:Fertilization / Q000187:drug effects / Q000502:physiology*; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice / Q000502:physiology*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*
chemical_list: D003451:Cryoprotective Agents; D019855:Ethylene Glycol
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