2,3,7,8-Tetrachlorodibenzo-p-dioxin up-regulates stathmin 1 in the eye: suggestive evidence for the involvement of stathmin 1 in accelerated eye opening in mice induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin.
When pregnant mice were exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the average time to eye opening in the offspring was shortened by about a day. How acceleration of eye opening by TCDD occurs remains unknown. To reveal the underlying mechanisms of the accelerated eye opening, pregnant mice were intraperitoneally injected with corn oil or TCDD at GD (gestation day) 11, and tissues around the eye of neonatal mice were subject to proteome analysis and RT-PCR. Upon TCDD administration, translationally controlled tumor protein (TCTP) and 60S acidic ribosomal protein p2 (RLA2) were reduced, while stathmin 1(STMN1) was increased, at both protein and mRNA levels. One hypothetical mechanism for eye opening is the proliferation of corneal epithelial cells before eye opening. STMN1, but not TCTP and RLA2, was up-regulated in immortalized human corneal epithelial cells (HCE-T) by TCDD, which promoted proliferation of HCE-T probably by accelerating the G1/S transition. Down-regulation of STMN1 by the antisense oligonucleotide technology inhibited proliferation of HCE-T, suggesting that STMN1, of which expression is enhanced by TCDD, may be involved in accelerated eye opening, probably by stimulating proliferation of corneal epithelial cells.
Gin
Dah-Won
DW
Department of Biology, Changwon National University, #9, Sarim-dong, Changwon, Kyungnam 641-773, South Korea.
Kim
Hye-Rin
HR
Lee
Dong-Min
DM
Lee
Seung-Hun
SH
Seong
Ah-Reum
AR
Hwang
Ju-Ae
JA
Park
Joo-Hung
JH
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D001564:Benzo(a)pyrene / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000378:metabolism; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000494:pharmacology*; D019573:Epithelium, Corneal / Q000187:drug effects* / Q000378:metabolism; D005123:Eye / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D006801:Humans; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000008:administration & dosage / Q000494:pharmacology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000187:drug effects / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D050777:Stathmin / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D000090324:Tumor Protein, Translationally-Controlled 1; D015854:Up-Regulation
D051792:Basic Helix-Loop-Helix Proteins; D001564:Benzo(a)pyrene; D014408:Biomarkers, Tumor; D004785:Environmental Pollutants; D016376:Oligonucleotides, Antisense; D010750:Phosphoproteins; D000072317:Polychlorinated Dibenzodioxins; D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon; D012269:Ribosomal Proteins; D050777:Stathmin; D000090324:Tumor Protein, Translationally-Controlled 1; C111235:AHR protein, human; C493995:STMN1 protein, human; C493994:Stmn1 protein, mouse; C000715647:TPT1 protein, human; C000715648:Tpt1 protein, mouse; C031937:phosphoprotein P2, ribosomal