title: Expression of TGF, matrix metalloproteinases, and tissue inhibitors in Chinese chronic rhinosinusitis.
abstract: BACKGROUND
  To date there is no information on the expression of mediators associated with tissue remodeling in Asian patients with chronic rhinitis with (CRSwNP) or without (CRSsNP) nasal polyps.
  
  
  OBJECTIVE
  To study the expression of TGF-beta1, matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), collagen, and regulatory T cells in Chinese patients with CRSwNP and CRSsNP.
  
  
  METHODS
  Thirty-six male and female subjects (12 patients with CRSwNP, 12 patients with CRSsNP, and 12 control subjects), age 17 to 60 years, were recruited into the study. Samples were collected from polyp and sinusoidal mucosal, ethmoidal mucosal, or inferior turbinate in the respective groups and assessed for TGF- beta1, MMP-2, MMP-7, MMP-9, TIMP-1, TIMP-2, TIMP-3, and TIMP-4 by immunoassay; collagen by histochemistry; and forkhead box P3 (FOXP3) mRNA by real-time PCR.
  
  
  RESULTS
  Patients with CRSwNP showed significantly lower concentrations of TGF-beta1, TIMP-1, TIMP-4, FOXP3, and collagen compared with patients with CRSsNP. Although there were no significant differences between the concentrations of MMP-7 and MMP-9 in patients with CRSwNP and CRSsNP, these were significantly increased compared with control patients. MMP-2 and TIMP-2 concentrations were not significantly different in any patient group, whereas TIMP-3 was not detectable.
  
  
  CONCLUSIONS
  Chronic rhinosinusitis with nasal polyps is characterized by a relative lack of TGF-ss expression versus CRSsNP. This finding may be causal for decreased collagen, TIMP-1/4, and FOXP3 expression in CRSwNP versus CRSsNP. TGF-ss serves as a main switch for different remodeling patterns in sinus disease.
authors.affiliation: Department of Oto-Rhino-Laryngology, West China Medical School, Sichuan University, Chengdu, Sichuan, 610041, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Meng
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Qiao
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Gabriele
authors.initials: G
authors.lastname: Holtappels
authors.forename: Ba
authors.initials: B
authors.lastname: Luo
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Zheng
authors.forename: Ping
authors.initials: P
authors.lastname: Lin
authors.forename: Shixi
authors.initials: S
authors.lastname: Liu
authors.forename: Claus
authors.initials: C
authors.lastname: Bachert
mesh_terms: D000293:Adolescent; D000328:Adult; D044466:Asian People; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008875:Middle Aged; D009298:Nasal Polyps / Q000150:complications / Q000276:immunology / Q000503:physiopathology; D012220:Rhinitis / Q000150:complications / Q000276:immunology / Q000503:physiopathology; D012852:Sinusitis / Q000150:complications / Q000276:immunology / Q000503:physiopathology; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*; D015291:Transforming Growth Factors / Q000378:metabolism*; D055815:Young Adult
chemical_list: D019714:Tissue Inhibitor of Metalloproteinases; D015291:Transforming Growth Factors; D020782:Matrix Metalloproteinases