Structure of the bacterial community in a biofilter during dimethyl sulfide (DMS) removal processes.
We report here both the successful treatment of DMS in a biofilter and the dynamic changes that occur in the composition of the bacterial community of the biofilter during this process. Denaturing gradient gel electrophoresis of eubacterial 16S rDNA samples taken from packing material at different DMS removal stages revealed 11 distinct bands. Phylogenetic analysis showed that the sequences of these bands were closest to sequences of species of the Proteobacteria, Firmicutes, and Actinobacteria. The specific occurrence of these bacterial species varied mainly with DMS load, but it was also affected by the addition of glucose and by ambient temperature. Based on the characteristics of the identified species, the system is conducive for such processes as sulfur oxidation, sulfate reduction, carbon oxidation, and fermentation. The strains identified in this study are potential candidates for purifying waste gas effluents containing DMS gas.
Chung
Ying-Chien
YC
Department of Biological Science and Technology, China University of Science and Technology, Taipei 115, Taiwan. ycchung@cc.cust.edu.tw
Cheng
Chiu-Yu
CY
Chen
Tzu-Yu
TY
Hsu
Jo-Shan
JS
Kui
Chun-Chi
CC
D001421:Bacteria, Anaerobic / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D013045:Species Specificity; D013440:Sulfides / Q000302:isolation & purification* / Q000378:metabolism*; D014462:Ultrafiltration / Q000379:methods*
D013440:Sulfides; C004784:dimethyl sulfide