title: Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions.
abstract: Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL(-1) of bFGF on 20 microg mL(-1) of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.
authors.affiliation: Department of Molecular & Medical Pharmacology, University of California, Los Angeles, CA 90095, USA. kkamei@mednet.ucla.edu
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Kamei
authors.forename: Minori
authors.initials: M
authors.lastname: Ohashi
authors.forename: Eric
authors.initials: E
authors.lastname: Gschweng
authors.forename: Quinn
authors.initials: Q
authors.lastname: Ho
authors.forename: Jane
authors.initials: J
authors.lastname: Suh
authors.forename: Jinghua
authors.initials: J
authors.lastname: Tang
authors.forename: Zeta Tak
authors.initials: ZT
authors.lastname: For Yu
authors.forename: Amander T
authors.initials: AT
authors.lastname: Clark
authors.forename: April D
authors.initials: AD
authors.lastname: Pyle
authors.forename: Michael A
authors.initials: MA
authors.lastname: Teitell
authors.forename: Ki-Bum
authors.initials: KB
authors.lastname: Lee
authors.forename: Owen N
authors.initials: ON
authors.lastname: Witte
authors.forename: Hsian-Rong
authors.initials: HR
authors.lastname: Tseng
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation*; D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D020869:Gene Expression Profiling / Q000295:instrumentation*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D039904:Pluripotent Stem Cells / Q000166:cytology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
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