title: Microbial pretreatment of corn stover with Ceriporiopsis subvermispora for enzymatic hydrolysis and ethanol production.
abstract: The feasibility of concurrent wet storage and microbial pretreatment of corn stover with Ceriporiopsis subvermispora for ethanol production was investigated in this study. The effects of particle size (5-15 mm), moisture content (45-85%), pretreatment time (18-35 d), and temperature (4-37 degrees C) on lignin degradation and enzymatic hydrolysis yield were studied. The results showed that C. subvermispora selectively degraded lignin up to 31.59% with a limited cellulose loss of less than 6% during an 18-d pretreatment. When 5mm corn stover was pretreated at 28 degrees C with 75% moisture content, overall glucose yields of 57.67%, 62.21%, and 66.61% were obtained with 18-, 28-, and 35-d microbial pretreated corn stover, respectively. For the above conditions, the highest overall ethanol yield of 57.80% was obtained with 35-d-pretreated corn stover. Enzymatic hydrolysis yield was highly related to the lignin removal during microbial pretreatment.
authors.affiliation: Department of Food, Agricultural, and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, Wooster, OH 44691-4096, USA.
authors.forename: Caixia
authors.initials: C
authors.lastname: Wan
authors.forename: Yebo
authors.initials: Y
authors.lastname: Li
mesh_terms: D002482:Cellulose / Q000378:metabolism; D055453:Coriolaceae / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D003313:Zea mays / Q000378:metabolism* / Q000382:microbiology
chemical_list: D004798:Enzymes; D000431:Ethanol; D002482:Cellulose; D005947:Glucose