title: Detection of hetero-proteins-mesoporous silica assembly by BRET.
abstract: The assembly of a hetero-protein (Renilla reniformis luciferase (Rluc) and a green fluorescence protein (sGFP)) encapsulated in folded-sheet mesoporous material with 7.1 nm pore diameter (FSM7.1), which was used for studying protein-protein interactions in pores of mesoporous silica, has been confirmed by the detection of bioluminescence resonance energy transfers (BRET).
authors.affiliation: Research Center for Compact Chemical Process, National Institute of Advanced Industrial Science and Technology (AIST), Nigatake 4-2-1, Miyagino-ku, Sendai, 983-8551, Japan. matsuura-shunichi@aist.go.jp
authors.forename: Shun-ichi
authors.initials: S
authors.lastname: Matsuura
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Tsunoda
authors.forename: Toru
authors.initials: T
authors.lastname: Shiomi
authors.forename: Kengo
authors.initials: K
authors.lastname: Sakaguchi
authors.forename: Takaaki
authors.initials: T
authors.lastname: Hanaoka
authors.forename: Fujio
authors.initials: F
authors.lastname: Mizukami
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008156:Luciferases / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D016062:Porosity; D011485:Protein Binding; D012822:Silicon Dioxide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D049452:Green Fluorescent Proteins; D012822:Silicon Dioxide; D008156:Luciferases