Assays of cell and axonemal motility in Chlamydomonas reinhardtii.
Chlamydomonas, an organism that offers a variety of flagella-deficient mutants, has been very important for studies of cilia and flagella. Motility assessment of mutant flagella at various levels helps us understand the function of specific axonemal proteins and structures for flagellar function. Measurements of gross cell movements are useful to assess the overall flagellar activity, analyses of demembranated and reactivated cells ("cell models") enable us to study the regulatory mechanism, and measurements of microtubule sliding velocity in vitro provide important information about dynein-microtubule interactions. This chapter describes fundamental techniques for these measurements.
Kamiya
Ritsu
R
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D054468:Axoneme / Q000502:physiology*; D002465:Cell Movement; D016825:Chlamydomonas reinhardtii / Q000502:physiology*; D005407:Flagella / Q000378:metabolism; D008297:Male; D008870:Microtubules / Q000378:metabolism; D012617:Sea Urchins; D013094:Spermatozoa / Q000166:cytology; D014741:Video Recording
D000255:Adenosine Triphosphate