Characteristics of a nickel-albumin binding assay for assessment of myocardial ischaemia.
BACKGROUND
The aim of this study was to describe a method to measure ischaemia-induced alterations of the binding capacity of serum albumin to exogenous nickel.


METHODS
We measured the levels of cardiac troponin I (cTnI), serum albumin, ischaemia-modified albumin (IMA) measured by a cobalt-albumin binding assay (CABA), and a nickel-albumin binding assay (NABA) in the following groups: myocardial infarction (n = 32) and non-ischaemic chest pain (n = 64).


RESULTS
IMA, cTnI and NABA levels were higher in the myocardial infarction group. NABA presented a higher ability to discriminate myocardial ischaemia than CABA.


CONCLUSIONS
Patients with myocardial infarction have reduced nickel binding to human serum albumin, and NABA may have an important role as an early marker of myocardial ischaemia.
da Silva
Sandra Huber
SH
Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Hausen
Bruna dos Santos
Bdos S
da Silva
Dievan Bisognin
DB
Becker
Aline Minuzzi
AM
de Campos
Marli Matiko Anraku
MM
Duarte
Marta Maria Medeiros Frescura
MM
Moresco
Rafael Noal
RN
D000368:Aged; D003035:Cobalt / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000175:diagnosis* / Q000378:metabolism; D009532:Nickel / Q000378:metabolism*; D012372:ROC Curve; D012709:Serum Albumin / Q000378:metabolism*; D019210:Troponin I / Q000097:blood; D020107:Troponin T / Q000097:blood
D012709:Serum Albumin; D019210:Troponin I; D020107:Troponin T; D003035:Cobalt; D009532:Nickel