title: DREB2C interacts with ABF2, a bZIP protein regulating abscisic acid-responsive gene expression, and its overexpression affects abscisic acid sensitivity.
abstract: ABF2 is a basic leucine zipper protein that regulates abscisic acid (ABA)-dependent stress-responsive gene expression. We carried out yeast two-hybrid screens to isolate genes encoding ABF2-interacting proteins in Arabidopsis (Arabidopsis thaliana). Analysis of the resulting positive clones revealed that two of them encode an AP2 domain protein, which is the same as AtERF48/DREB2C. This protein, which will be referred to as DREB2C, could bind C-repeat/dehydration response element in vitro and possesses transcriptional activity. To determine its function, we generated DREB2C overexpression lines and investigated their phenotypes. The transgenic plants were ABA hypersensitive during germination and seedling establishment stages, whereas primary root elongation of seedlings was ABA insensitive, suggesting developmental stage dependence of DREB2C function. The DREB2C overexpression lines also displayed altered stress response; whereas the plants were dehydration sensitive, they were freezing and heat tolerant. We further show that other AP2 domain proteins, DREB1A and DREB2A, interact with ABF2 and that other ABF family members, ABF3 and ABF4, interact with DREB2C. Previously, others demonstrated that ABF and DREB family members cooperate to activate the transcription of an ABA-responsive gene. Our result implies that the cooperation of the two classes of transcription factors may involve physical interaction.
authors.affiliation: Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Sun-Ji
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jung-Youn
authors.initials: JY
authors.lastname: Kang
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Myoung Duck
authors.initials: MD
authors.lastname: Kim
authors.forename: Min Seok
authors.initials: MS
authors.lastname: Bae
authors.forename: Hyung-In
authors.initials: HI
authors.lastname: Choi
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Kim
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D003681:Dehydration; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018525:Germination; D006358:Hot Temperature; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D036226:Seedlings / Q000254:growth & development; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D000040:Abscisic Acid; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D018749:RNA, Plant; D014157:Transcription Factors; C508877:At1g45249 protein, Arabidopsis
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