The effects of different maceration techniques on nuclear DNA amplification using human bone.
Forensic anthropologists routinely macerate human bone for the purposes of identity and trauma analysis, but the heat and chemical treatments used can destroy genetic evidence. As a follow-up to a previous study on nuclear DNA recovery that used pig ribs, this study utilizes human skeletal remains treated with various bone maceration techniques for nuclear DNA amplification using the standard Combined DNA Index System (CODIS) markers. DNA was extracted from 18 samples of human lower leg bones subjected to nine chemical and heat maceration techniques. Genotyping was carried out using the AmpFlSTR COfiler and AmpFlSTR Profiler Plus ID kits. Results showed that heat treatments via microwave or Biz/Na(2)CO(3) in sub-boiling water efficiently macerate bone and produce amplifiable nuclear DNA for genetic analysis. Long-term use of chemicals such as hydrogen peroxide is discouraged as it results in poor bone quality and has deleterious effects on DNA amplification.
Lee
Esther J
EJ
Department of Anthropology, Binghamton University, SUNY, PO Box 6000, Binghamton, NY 13902-6000, USA.
Luedtke
Jennifer G
JG
Allison
Jamie L
JL
Arber
Carolyn E
CE
Merriwether
D Andrew
DA
Steadman
Dawnie Wolfe
DW
D015519:Bone Density; D002254:Carbonates; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D003902:Detergents; D004202:Disinfectants; D004338:Drug Combinations; D005360:Fibula; D053803:Forensic Genetics; D005819:Genetic Markers; D005838:Genotype; D006358:Hot Temperature; D006801:Humans; D006861:Hydrogen Peroxide; D007101:Immersion; D008872:Microwaves; D009812:Odorants; D010206:Papain; D016133:Polymerase Chain Reaction; D012973:Sodium Hypochlorite; D012982:Sodium, Dietary; D013048:Specimen Handling / Q000379:methods*; D020080:Tandem Repeat Sequences; D013977:Tibia
D002254:Carbonates; D003902:Detergents; D004202:Disinfectants; D004338:Drug Combinations; D005819:Genetic Markers; D012982:Sodium, Dietary; C032508:meat tenderizer; C005686:sodium carbonate; D004247:DNA; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D010206:Papain