title: Standardized preprocessing of urine for proteome analysis.
abstract: Proteome/peptidome profiling of human urine is a promising tool for the discovery of novel disease-associated biomarkers. However, a wide range of preanalytic variables influence the results of proteome/peptidome analysis regardless of the method used. We present a validated pretreatment protocol, which allows standardization of preanalytic modalities and facilitates reproducible peptidome profiling of human urine by means of magnetic bead (MB) separation in combination with MALDI-TOF MS. Such a procedure is necessary for generating consistent and reliable data from which meaningful results may be obtained for biomarker discovery and general proteomic experiments.
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, Medical Faculty, University Leipzig, Leipzig, Germany.
authors.forename: Georg Martin
authors.initials: GM
authors.lastname: Fiedler
authors.forename: Uta
authors.initials: U
authors.lastname: Ceglarek
authors.forename: Alexander
authors.initials: A
authors.lastname: Leichtle
authors.forename: Joachim
authors.initials: J
authors.lastname: Thiery
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods* / Q000592:standards*; D002138:Calibration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D040901:Proteomics / Q000379:methods*; D013048:Specimen Handling; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016482:Urinalysis / Q000379:methods*