Using buoyant mass to measure the growth of single cells.
We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.
Godin
Michel
M
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, USA.
Delgado
Francisco Feijó
FF
Son
Sungmin
S
Grover
William H
WH
Bryan
Andrea K
AK
Tzur
Amit
A
Jorgensen
Paul
P
Payer
Kris
K
Grossman
Alan D
AD
Kirschner
Marc W
MW
Manalis
Scott R
SR
D000818:Animals; D001412:Bacillus subtilis / Q000254:growth & development; D002453:Cell Cycle; D049108:Cell Enlargement; D004926:Escherichia coli / Q000254:growth & development; D008214:Lymphocytes / Q000502:physiology; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000254:growth & development
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