title: Involvement of recF in 254 nm ultraviolet radiation resistance in Deinococcus radiodurans and Escherichia coli.
abstract: recF is a critical gene involved in the RecFOR pathway of DNA repair in Deinococcus radiodurans (D. radiodurans). To investigate the role of recF in UV-C radiation resistance, we generated the recF-deficient D. radiodurans strain R1, expressed recF in Escherichia coli (E. coli) BL21 cells, and compared the ability of each to resist UV-C radiation by F (10), inactivation constant (IC), and extrapolation number (N). The mutation of recF in D. radiodurans R1 resulted in characteristic slow growth and dramatic sensitivity to UV-C irradiation. Transformation of recF into E. coli BL21 cells resulted in increased UV-C resistance compared to untransformed E. coli BL21 cells. These results suggested that recF is needed for the replication of D. radiodurans. Furthermore, as a part of the RecFOR pathway in D. radiodurans, disruption of recF could dramatically decrease the UV-C resistance of D. radiodurans and recF could increase the UV-C resistance of E. coli BL21 cells.
authors.affiliation: Department of Environmental Hygiene, Third Military Medical University, Chongqing, China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Chang
authors.forename: Lan
authors.initials: L
authors.lastname: Yang
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Fu
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Zhiqun
authors.initials: Z
authors.lastname: Qiu
authors.forename: Ji-an
authors.initials: JA
authors.lastname: Chen
authors.forename: Ran
authors.initials: R
authors.lastname: Hu
authors.forename: Weiqun
authors.initials: W
authors.lastname: Shu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology*; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D034301:Deinococcus / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D005784:Gene Amplification; D042822:Genomic Instability; D009154:Mutation; D016133:Polymerase Chain Reaction; D011836:Radiation Tolerance / Q000235:genetics*; D011995:Recombination, Genetic; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C066218:recF protein, Bacteria
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