title: First description of the adult stage of Clinostomum cutaneum Paperna, 1964 (Digenea: Clinostomidae) from grey herons Ardea cinerea L. and a redescription of the metacercaria from the Nile tilapia Oreochromis niloticus niloticus (L.) in Kenya.
abstract: The combined use of morphological and molecular studies allowed for the first time the recognition and description of the adult stage of Clinostomum cutaneum Paperna, 1964 from the grey heron Ardea cinerea L. in Kenya. A redescription of the metacercaria that infect Nile tilapia Oreochromis niloticus niloticus (L.) from the same aquatic environment is also presented. C. cutaneum differs from all other species of Clinostomum Leidy, 1856 in the shape of its uterus. Sequencing the rRNA confirmed the morphological similarity between adults from the grey heron and the metacercarial stage from tilapia, and a level of genetic similarity with the other previously sequenced Clinostomum spp. was observed. The need for a reorganisation of Clinostomum using both morphological and molecular methods is highlighted.
authors.affiliation: Department of Veterinary Public Health and Animal Pathology, University of Bologna, via Tolara di Sopra 50, 40064, Ozzano Emilia, BO, Italy. andrea.gustinelli2@unibo.it
authors.forename: Andrea
authors.initials: A
authors.lastname: Gustinelli
authors.forename: Monica
authors.initials: M
authors.lastname: Caffara
authors.forename: Daniela
authors.initials: D
authors.lastname: Florio
authors.forename: Elick O
authors.initials: EO
authors.lastname: Otachi
authors.forename: Euty M
authors.initials: EM
authors.lastname: Wathuta
authors.forename: Maria L
authors.initials: ML
authors.lastname: Fioravanti
mesh_terms: D000818:Animals; D001717:Birds / Q000469:parasitology*; D023681:Cichlids / Q000469:parasitology*; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D007630:Kenya; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014200:Trematoda / Q000033:anatomy & histology / Q000145:classification* / Q000302:isolation & purification*
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S
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