title: The differential gene expression profiles between sensitive and resistant breast cancer cells to adriamycin by cDNA microarray.
abstract: OBJECTIVE
  Adriamycin is one of the most commonly used drugs in the treatment of breast cancer. This study was performed to understand the molecular mechanisms of drug resistance in breast cancer cells.
  
  
  METHODS
  We have analyzed the MCF-7 breast cell line and its adriamycin-resistant variants, MCF-7/ADR using human 10 K element cDNA microarrays.
  
  
  RESULTS
  We defined 68 genes that were up-regulated (14 genes) or down-regulated (54 genes) in adriamycin resistant breast cancer cells. Several genes, such as G protein-coupled receptor kinase 5, phospholipase A2, guanylate cyclase 1, vimentin, matrix metalloproteinase 1 are up-regulated in drug resistant cells. Several genes, such as interferon, alpha-inducible protein 27, forkhead box M1, mitogen-activated protein kinase 6, regulator of mitotic spindle assembly 1 and tumor necrosis factor superfamily are down-regulated in adriamycin resistant cells. The altered expression of genes observed in microarray was verified by RT-PCR.
  
  
  CONCLUSIONS
  These findings show that cDNA microarray analysis can be used to obtain gene expression profiles reflecting the effect of anticancer drugs on breast cancer cells. Such data may lead to the assigning of signature expression profiles of drug-resistant tumors which may help predict responses to drugs and assist in the design of tailored therapeutic regimens to overcome drug resistance.
authors.affiliation: Department of Internal Medicine, College of Medicine, Hanyang University, Seoul, Korea. ahnmj@hanyang.ac.kr
authors.forename: Myung-Ju
authors.initials: MJ
authors.lastname: Ahn
authors.forename: Ki-Hwan
authors.initials: KH
authors.lastname: Lee
authors.forename: Joon-Ik
authors.initials: JI
authors.lastname: Ahn
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Yu
authors.forename: Hye-Sook
authors.initials: HS
authors.lastname: Lee
authors.forename: Jung-Hye
authors.initials: JH
authors.lastname: Choi
authors.forename: Joung Soon
authors.initials: JS
authors.lastname: Jang
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Bae
authors.forename: Yong-Sung
authors.initials: YS
authors.lastname: Lee
references.citation: Biochem Pharmacol. 1998 Aug 15;56(4):451-7
references.pmid: 9763220
references.citation: Anal Biochem. 1983 Mar;129(2):517-21
references.pmid: 6303162
references.citation: Annu Rev Biochem. 1990;59:569-96
references.pmid: 2165384
references.citation: Cancer Res. 2001 Jan 15;61(2):577-81
references.pmid: 11212252
references.citation: Cancer Res. 1991 May 15;51(10):2720-6
references.pmid: 1673639
references.citation: Int J Mol Med. 1998 Feb;1(2):491-4
references.pmid: 9852255
references.citation: Trends Cell Biol. 2000 Sep;10(9):369-77
references.pmid: 10932094
references.citation: Cancer Res. 1989 Jul 15;49(14):3844-8
references.pmid: 2544260
references.citation: Gen Pharmacol. 1996 Mar;27(2):251-5
references.pmid: 8919638
references.citation: Biochem Pharmacol. 2002 Mar 1;63(5):823-31
references.pmid: 11911833
references.citation: Blood. 1999 Mar 1;93(5):1658-67
references.pmid: 10029595
references.citation: Leukemia. 1999 Nov;13(11):1854-8
references.pmid: 10557062
references.citation: J Pharmacol Exp Ther. 2001 Nov;299(2):434-41
references.pmid: 11602652
references.citation: Biochem Biophys Res Commun. 2002 Sep 6;296(5):1125-33
references.pmid: 12207890
references.citation: Annu Rev Biochem. 1988;57:593-625
references.pmid: 3052284
references.citation: Nat Rev Cancer. 2002 Jun;2(6):420-30
references.pmid: 12189384
references.citation: Cancer Res Treat. 2003 Oct;35(5):451-9
references.pmid: 26680973
references.citation: Oncogene. 2001 Mar 15;20(11):1300-6
references.pmid: 11313874
references.citation: Cancer Res. 2000 Aug 1;60(15):4161-6
references.pmid: 10945624
references.citation: Nat Genet. 1996 Dec;14(4):457-60
references.pmid: 8944026
references.citation: Br J Cancer. 1995 Mar;71(3):505-11
references.pmid: 7880731
references.citation: J Bioenerg Biomembr. 1997 Apr;29(2):151-63
references.pmid: 9239540
references.citation: Cancer Res. 1992 Oct 1;52(19):5190-7
references.pmid: 1382837
references.citation: Hum Cell. 2001 Sep;14(3):211-21
references.pmid: 11774740
references.citation: Semin Hematol. 1997 Oct;34(4 Suppl 5):3-8
references.pmid: 9408955