Airway mast cells in a rhesus model of childhood allergic airways disease.
Asthma is a leading cause of morbidity in children. Risk factors include chronic exposure to allergens and air pollution. While chronically activated mast cells contribute to the pathophysiology of asthma in part through their proteases such as chymase and tryptase, previous studies of airway mast cell abundance and distribution in asthmatics have been inconsistent. To determine whether repeated episodic exposures to environmental pollutants during postnatal lung development alter airway mast cell abundance and distribution, we exposed infant rhesus monkeys to a known human allergen, house dust mite antigen (HDMA), and/or a known environmental pollutant, ozone (O(3)), and quantitatively compared the abundance of tryptase- or chymase-positive mast cells in three airway levels. Mast cells are resident in multiple compartments of the airway wall in infant rhesus monkeys raised from birth in filtered air. Tryptase- and chymase-positive cells were most abundant in trachea and least in terminal bronchioles. The majority of tryptase-positive and almost all chymase-positive cells were in extracellular matrix and smooth muscle bundles. Chronic exposure to HDMA elevated the abundance of both tryptase- and chymase-positive cells in the trachea and intrapulmonary bronchi. Neither exposure to O(3) nor HDMA + O(3) increased mast cell accumulations in the airway wall. We conclude that during postnatal airway development (1) mast cells are a resident airway cell population even in the absence of toxic air contaminants; (2) aeroallergen exposure alters large airway mast cell distribution and abundance, increasing chymase-positive mast cells; and (3) this response is attenuated by exposure to oxidant air pollutants.
Van Winkle
Laura S
LS
Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, California 95616, USA. lsvanwinkle@ucdavis.edu
Baker
Gregory L
GL
Chan
Jackie K W
JK
Schelegle
Edward S
ES
Plopper
Charles G
CG
D000818:Animals; D002648:Child; D006801:Humans; D006967:Hypersensitivity / Q000473:pathology*; D007150:Immunohistochemistry; D008253:Macaca mulatta; D008407:Mast Cells / Q000473:pathology*; D014132:Trachea / Q000473:pathology*
Curr Opin Allergy Clin Immunol. 2007 Feb;7(1):43-50
17218810
Respir Physiol Neurobiol. 2007 Jan 15;155(1):55-63
16616710
Am J Respir Crit Care Med. 1998 Feb;157(2):403-9
9476850
Am J Respir Cell Mol Biol. 1999 Dec;21(6):655-7
10572061
Am J Clin Pathol. 1971 Mar;55(3):283-90
5549896
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3739-43
8170980
Am Rev Respir Dis. 1990 Oct;142(4):863-71
2221594
Am J Physiol Lung Cell Mol Physiol. 2006 Oct;291(4):L644-50
16648242
Am Rev Respir Dis. 1988 Dec;138(6 Pt 2):S31-4
2462390
FASEB J. 2003 Nov;17(14):2139-41
14500550
J Allergy Clin Immunol. 2007 Aug;120(2):329-33
17559912
Am J Respir Crit Care Med. 1997 Feb;155(2):654-60
9032209
Toxicol Appl Pharmacol. 2006 Aug 1;214(3):237-43
16466656
Environ Health Perspect. 2006 Jun;114(6):911-6
16759994
J Clin Invest. 2006 Mar;116(3):783-96
16511606
J Immunol. 2009 Nov 15;183(10):6369-76
19841188
Clin Rev Allergy Immunol. 2002 Apr;22(2):161-74
11975421
J Allergy Clin Immunol. 2002 Jun;109(6 Suppl):S521-4
12063507
Lancet. 2002 Feb 2;359(9304):386-91
11844508
Am J Respir Crit Care Med. 2005 Mar 1;171(5):431-9
15563633
Am J Respir Crit Care Med. 2006 Nov 15;174(10):1069-76
16931639
Toxicol Appl Pharmacol. 2003 Aug 15;191(1):74-85
12915105
Crit Rev Oncol Hematol. 1999 Jul;31(2):119-33
10451798
N Engl J Med. 2002 May 30;346(22):1699-705
12037149
Respir Res. 2008 Sep 10;9:64
18783610
Int Arch Allergy Immunol. 2001 Jan-Mar;124(1-3):146-50
11306952
J Immunol. 1999 Sep 1;163(5):2799-808
10453024
J Biol Chem. 1994 Jan 28;269(4):2695-702
8300599
Toxicol Pathol. 2007 Jan;35(1):97-110
17325978
J Immunol. 1994 Oct 15;153(8):3717-23
7523504
J Allergy Clin Immunol. 2006 Jun;117(6):1277-84
16750987
Clin Sci (Lond). 1994 Aug;87(2):151-63
7924160
J Allergy Clin Immunol. 2005 Jun;115(6):1162-8
15940129
Am J Respir Cell Mol Biol. 2005 Jul;33(1):1-8
15834047
J Immunol. 2009 Jun 15;182(12):7430-9
19494266
Blood. 2000 Mar 15;95(6):2052-8
10706874
Invest Ophthalmol Vis Sci. 2001 Apr;42(5):995-1001
11274077
J Allergy Clin Immunol. 2004 Nov;114(5):997-1008; quiz 1009
15536399
J Biol Chem. 1997 Mar 14;272(11):7127-31
9054407
Clin Exp Allergy. 2005 Jul;35(7):894-906
16008676
Am Rev Respir Dis. 1989 Mar;139(3):806-17
2923380
J Immunol. 2002 Jul 15;169(2):1014-20
12097409
J Allergy Clin Immunol. 1991 Oct;88(4):661-74
1918731
Am J Pathol. 2001 Jan;158(1):333-41
11141508
J Cell Physiol. 1998 Aug;176(2):365-73
9648924
J Occup Environ Med. 2005 Dec;47(12):1285-91
16340710
Am Rev Respir Dis. 1993 Mar;147(3):684-9
7680188
JAMA. 1971 Feb 1;215(5):769-76
5107537
Clin Exp Allergy. 2004 Oct;34(10):1627-33
15479280