Suppression of PTP1B in gastric cancer cells in vitro induces a change in the genome-wide expression profile and inhibits gastric cancer cell growth.
PTP1B (protein tyrosine phosphatase 1B) is a member of the superfamily of PTPs (protein tyrosine phosphatases) and has been implicated in cancer pathogenesis. However, the role of PTP1B in gastric cancer is still unknown. Here, we first detected the PTP1B expression in six gastric cancer cell lines and in the immortalized gastric mucosal epithelial cell line GES-1 by RT-PCR and Western blot. Then, we measured the change of the genome-wide expression profile in MKN28 gastric cancer cells transfected with a plasmid expressing PTP1B-specific small interfering RNA by microarray analysis. Our results showed that PTP1B was overexpressed in gastric cancer cells, and inhibition of PTP1B expression dramatically inhibited gastric cancer cell growth in vitro and in vivo. In addition, microarray analysis revealed that inhibition of PTP1B induced changes in the genome-wide expression profile. These changes may be related to cell growth. Taken together, our data suggested that PTP1B may be a candidate oncogene in gastric cancer.
Wang
Jinguo
J
Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 20025, Peoples Republic of China.
Chen
Xuehua
X
Liu
Bingya
B
Zhu
Zhenggang
Z
D000818:Animals; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D006801:Humans; D046228:Microarray Analysis; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology
D034741:RNA, Small Interfering; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1