title: Laser-induced propagation and destruction of amyloid beta fibrils.
abstract: The amyloid deposition of amyloid beta (Abeta) peptides is a critical pathological event in Alzheimer disease (AD). Preventing the formation of amyloid deposits and removing preformed fibrils in tissues are important therapeutic strategies against AD. Previously, we reported the destruction of amyloid fibrils of beta(2)-microglobulin K3 fragments by laser irradiation coupled with the binding of amyloid-specific thioflavin T. Here, we studied the effects of a laser beam on Abeta fibrils. As was the case for K3 fibrils, extensive irradiation destroyed the preformed Abeta fibrils. However, irradiation during spontaneous fibril formation resulted in only the partial destruction of growing fibrils and a subsequent explosive propagation of fibrils. The explosive propagation was caused by an increase in the number of active ends due to breakage. The results not only reveal a case of fragmentation-induced propagation of fibrils but also provide insights into therapeutic strategies for AD.
authors.affiliation: Institute for Protein Research, Osaka University, Yamadaoka 3-2, Suita, Osaka 565-0871, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Yagi
authors.forename: Daisaku
authors.initials: D
authors.lastname: Ozawa
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Sakurai
authors.forename: Toru
authors.initials: T
authors.lastname: Kawakami
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kuyama
authors.forename: Osamu
authors.initials: O
authors.lastname: Nishimura
authors.forename: Toshinori
authors.initials: T
authors.lastname: Shimanouchi
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Kuboi
authors.forename: Hironobu
authors.initials: H
authors.lastname: Naiki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Goto
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000628:therapy*; D000682:Amyloid / Q000737:chemistry*; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D052160:Benzothiazoles; D006801:Humans; D007700:Kinetics; D007834:Lasers; D008954:Models, Biological; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010778:Photochemotherapy / Q000379:methods; D017510:Protein Folding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013844:Thiazoles / Q000737:chemistry; D014461:Ultracentrifugation; D001613:beta 2-Microglobulin / Q000737:chemistry
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D010446:Peptide Fragments; D010455:Peptides; D013844:Thiazoles; D001613:beta 2-Microglobulin; C009462:thioflavin T
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