title: Phenotypic and genetic characterization of antimicrobial profiles of Helicobacter pylori strains in Cuba.
abstract: The study evaluated the antibiotic resistance patterns of Helicobacter pylori strains against metronidazole and clarithromycin in a hospital in Havana, Cuba. Eighty-five percent, 22.5%, and 10% of 40 H. pylori strains investigated were resistant to metronidazole, ciprofloxacin, and clarithromycin respectively but all were susceptible to amoxicillin and tetracycline. RdxA truncation was found only in metronidazole-resistant strains. In such strains, reported are eight and two novel mutations in the rdxA and frxA genes respectively. Two-point mutations in the 23S rRNA genes of clarithromycin-resistant strains were detected. A high prevalence of metronidazole resistance was found in Cuban H. pylori strains. Mutations in the rdxA gene may contribute more significantly than frxA gene to the high level of resistance to metronidazole. This study supports the need to continue monitoring the antibiotic susceptibility in H. pylori in Cuba to guide the treatment of such infection.
authors.affiliation: Microbiology, Clinical and Epidemiology Branch, Institute Pedro Kouri, Havana, Cuba. llanes@ipk.sld.cu
authors.forename: Rafael
authors.initials: R
authors.lastname: Llanes
authors.forename: Claudia
authors.initials: C
authors.lastname: Soria
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nagashima
authors.forename: Nobumichi
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Angela
authors.initials: A
authors.lastname: Gala
authors.forename: Daymi
authors.initials: D
authors.lastname: Guzmán
authors.forename: Onelkis
authors.initials: O
authors.lastname: Feliciano
authors.forename: Lidunka
authors.initials: L
authors.lastname: Valdés
authors.forename: Oderay
authors.initials: O
authors.lastname: Gutiérrez
authors.forename: Heriberto
authors.initials: H
authors.lastname: Fernández
authors.forename: Alina
authors.initials: A
authors.lastname: Llop
authors.forename: Akihiro
authors.initials: A
authors.lastname: Wada
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000658:Amoxicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology; D017291:Clarithromycin / Q000494:pharmacology; D003462:Cuba; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005260:Female; D016480:Helicobacter pylori / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D012737:Sex Factors; D013045:Species Specificity; D013752:Tetracycline / Q000494:pharmacology; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D008795:Metronidazole; D002939:Ciprofloxacin; D000658:Amoxicillin; D013752:Tetracycline; D017291:Clarithromycin
references.citation: Helicobacter. 2006 Oct;11(5):441-5
references.pmid: 16961806
references.citation: J Antimicrob Chemother. 1999 Jan;43(1):47-54
references.pmid: 10381100
references.citation: Aliment Pharmacol Ther. 2006 Feb 1;23(3):429-35
references.pmid: 16423002
references.citation: Clin Gastroenterol Hepatol. 2003 Jul;1(4):273-8
references.pmid: 15017668
references.citation: Antimicrob Agents Chemother. 2001 Sep;45(9):2609-15
references.pmid: 11502537
references.citation: Aliment Pharmacol Ther. 2009 Jan;29(2):145-60
references.pmid: 18945250
references.citation: Antimicrob Agents Chemother. 2001 Mar;45(3):727-33
references.pmid: 11181351
references.citation: Vet Microbiol. 2007 Sep 20;124(1-2):16-24
references.pmid: 17467929
references.citation: J Clin Pathol. 2008 Oct;61(10):1112-5
references.pmid: 18755715
references.citation: Helicobacter. 2004 Oct;9(5):400-7
references.pmid: 15361078
references.citation: Sex Transm Dis. 2003 Jan;30(1):10-4
references.pmid: 12514435
references.citation: J Antimicrob Chemother. 2006 Jan;57(1):71-8
references.pmid: 16284224
references.citation: J Clin Microbiol. 2004 Oct;42(10):4856-8
references.pmid: 15472362
references.citation: Emerg Infect Dis. 2004 Jun;10(6):1088-94
references.pmid: 15207062
references.citation: Int J Antimicrob Agents. 2007 Apr;29(4):389-96
references.pmid: 17303392
references.citation: Helicobacter. 2004;9 Suppl 1:67-72
references.pmid: 15347308
references.citation: Gut. 2004 Sep;53(9):1374-84
references.pmid: 15306603