Novel in vivo model of inducible multi-drug resistance in acute lymphoblastic leukemia with chromosomal translocation t(4;11).
Acute lymphoblastic leukemia (ALL) with translocation t(4;11) is found in 60-85% of infants with ALL and is often refractory to conventional chemotherapeutics after relapse. Using the t(4;11) ALL line SEM, we evaluated chemotherapy resistance in NOD.CB17-Prkdcscid/J mice. SEM cells were injected into the tail vein and engraftment was monitored by flow cytometry. Once engraftment was observed, mice were injected intraperitoneally with phosphate-buffered saline (PBS), or vincristine (0.5mg/kg body weight) three times per week for 4weeks (n=8 per group). The level of P-glycoprotein in SEM cells was increased 3-fold by vincristine treatment compared to PBS-treated mice. Survival curves showed that leukemia cell growth was initially delayed by vincristine treatment, but the mice eventually succumbed to disease. These data describe a novel inducible model for investigating multi-drug resistance mechanisms in high-risk t(4;11) ALL.
Zunino
Susan J
SJ
United States Department of Agriculture, Agricultural Research Service, Western Human Nutrition Research Center, 430 West Health Sciences Drive, Davis, CA 95616, United States. susan.zunino@ars.usda.gov
Storms
David H
DH
Ducore
Jonathan M
JM
D000328:Adult; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002880:Chromosomes, Human, Pair 11; D002894:Chromosomes, Human, Pair 4; D003433:Crosses, Genetic; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic / Q000235:genetics*; D043222:Ubiquitin Thiolesterase / Q000235:genetics; D014750:Vincristine / Q000627:therapeutic use
C482860:UCHL1 protein, human; D014750:Vincristine; D043222:Ubiquitin Thiolesterase
Cancer Res. 1996 Oct 1;56(19):4332-7
8813118
J Cancer Res Clin Oncol. 2007 Nov;133(11):875-93
17671794
Cancer Res. 1988 Jan 15;48(2):393-8
2891436
Annu Rev Biochem. 1993;62:385-427
8102521
Blood. 1997 Sep 1;90(5):2015-9
9292537
Cancer Res. 1993 Aug 15;53(16):3658-61
8101765
Leukemia. 1996 Mar;10(3):558-63
8642875
Blood. 2004 May 15;103(10):3905-14
14764536
Blood. 1996 May 1;87(9):3892-8
8611717
Cancer Chemother Pharmacol. 2009 Apr;63(5):761-7
19034447
Klin Padiatr. 1997 Jul-Aug;209(4):178-85
9293448
Science. 1989 Dec 22;246(4937):1597-600
2595371
J Immunol Methods. 1991 Jun 3;139(2):271-9
1710634
Blood. 2002 Jun 1;99(11):4100-8
12010813
Blood. 1996 Aug 15;88(4):1135-46
8695830
Biochem Biophys Res Commun. 1986 Dec 30;141(3):956-62
2880583
Pediatr Hematol Oncol. 2000 Dec;17(8):635-50
11127395
Leuk Lymphoma. 2003 Jan;44(1):85-95
12691146
Crit Rev Clin Lab Sci. 1998 Feb;35(1):1-57
9532418
N Engl J Med. 1998 Jun 4;338(23):1663-71
9614257
Br J Haematol. 1994 Feb;86(2):275-83
8199015
Blood. 1998 Jun 1;91(11):3995-4019
9596644
Int J Clin Pharmacol Ther. 2000 Mar;38(3):94-110
10739113
Blood. 1998 Mar 15;91(6):2092-8
9490695
Nat Rev Cancer. 2003 Sep;3(9):639-49
12951583
Leuk Lymphoma. 2005 May;46(5):681-91
16019505