title: Transgenerational inheritance and resetting of stress-induced loss of epigenetic gene silencing in Arabidopsis.
abstract: Plants, as sessile organisms, need to sense and adapt to heterogeneous environments and have developed sophisticated responses by changing their cellular physiology, gene regulation, and genome stability. Recent work demonstrated heritable stress effects on the control of genome stability in plants--a phenomenon that was suggested to be of epigenetic nature. Here, we show that temperature and UV-B stress cause immediate and heritable changes in the epigenetic control of a silent reporter gene in Arabidopsis. This stress-mediated release of gene silencing correlated with pronounced alterations in histone occupancy and in histone H3 acetylation but did not involve adjustments in DNA methylation. We observed transmission of stress effects on reporter gene silencing to non-stressed progeny, but this effect was restricted to areas consisting of a small number of cells and limited to a few non-stressed progeny generations. Furthermore, stress-induced release of gene silencing was antagonized and reset during seed aging. The transient nature of this phenomenon highlights the ability of plants to restrict stress-induced relaxation of epigenetic control mechanisms, which likely contributes to safeguarding genome integrity.
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Applied Life Sciences and Natural Resources (BOKU), Muthgasse 18, 1190 Vienna, Austria.
authors.forename: Christina
authors.initials: C
authors.lastname: Lang-Mladek
authors.forename: Olga
authors.initials: O
authors.lastname: Popova
authors.forename: Kathrin
authors.initials: K
authors.lastname: Kiok
authors.forename: Marc
authors.initials: M
authors.lastname: Berlinger
authors.forename: Branislava
authors.initials: B
authors.lastname: Rakic
authors.forename: Werner
authors.initials: W
authors.lastname: Aufsatz
authors.forename: Claudia
authors.initials: C
authors.lastname: Jonak
authors.forename: Marie-Theres
authors.initials: MT
authors.lastname: Hauser
authors.forename: Christian
authors.initials: C
authors.lastname: Luschnig
mesh_terms: D000107:Acetylation; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation / Q000502:physiology; D044127:Epigenesis, Genetic / Q000235:genetics*; D020868:Gene Silencing / Q000502:physiology; D006657:Histones / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002843:Chromatin; D006657:Histones
references.citation: EMBO J. 2000 Sep 1;19(17):4431-8
references.pmid: 10970837
references.citation: PLoS Genet. 2009 Sep;5(9):e1000638
references.pmid: 19730687
references.citation: Plant J. 2006 Apr;46(1):124-33
references.pmid: 16553900
references.citation: Curr Biol. 2000 Dec 14-28;10(24):1591-4
references.pmid: 11137011
references.citation: Biochem J. 2007 Nov 15;408(1):113-21
references.pmid: 17666011
references.citation: Plant Cell. 2004 Apr;16(4):1021-34
references.pmid: 15037732
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: PLoS One. 2009;4(4):e5202
references.pmid: 19381297
references.citation: Mol Plant. 2009 Jul;2(4):610-627
references.pmid: 19825643
references.citation: Science. 2005 Jun 3;308(5727):1466-9
references.pmid: 15933200
references.citation: EMBO J. 2006 Dec 13;25(24):5735-41
references.pmid: 17139253
references.citation: Genome Res. 2008 Mar;18(3):469-76
references.pmid: 18218979
references.citation: EMBO J. 2006 Jun 21;25(12):2828-36
references.pmid: 16724114
references.citation: Curr Opin Plant Biol. 2007 Jun;10(3):317-22
references.pmid: 17468039
references.citation: Mol Plant. 2009 Jul;2(4):724-737
references.pmid: 19825652
references.citation: Mol Plant. 2008 Jan;1(1):118-28
references.pmid: 20031919
references.citation: Genome Biol. 2002 Jun 18;3(7):RESEARCH0034
references.pmid: 12184808
references.citation: Mol Plant. 2009 Jul;2(4):675-687
references.pmid: 19825648
references.citation: Plant J. 2001 Nov;28(4):385-95
references.pmid: 11737776
references.citation: Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4945-50
references.pmid: 18356294
references.citation: EMBO J. 2002 Dec 16;21(24):6832-41
references.pmid: 12486004
references.citation: Anal Biochem. 2008 Apr 15;375(2):354-60
references.pmid: 18249178
references.citation: PLoS Genet. 2006 Apr;2(4):e65
references.pmid: 16683039
references.citation: J Biol Chem. 2003 Feb 7;278(6):4035-40
references.pmid: 12427740
references.citation: Annu Rev Plant Biol. 2008;59:387-415
references.pmid: 18257711
references.citation: Dev Cell. 2004 Nov;7(5):653-62
references.pmid: 15525527
references.citation: Plant Cell Physiol. 2008 Oct;49(10):1580-8
references.pmid: 18779215
references.citation: Nature. 2000 Jul 6;406(6791):98-101
references.pmid: 10894550
references.citation: Nucleic Acids Res. 2007;35(5):1714-25
references.pmid: 17311811
references.citation: Cell. 1999 Nov 24;99(5):451-4
references.pmid: 10589672
references.citation: Nat Neurosci. 2004 Aug;7(8):847-54
references.pmid: 15220929
references.citation: Cell. 2007 Feb 23;128(4):655-68
references.pmid: 17320504
references.citation: Curr Biol. 2009 Oct 13;19(19):1677-81
references.pmid: 19781944
references.citation: Nat Genet. 2002 Mar;30(3):311-4
references.pmid: 11836502
references.citation: Curr Opin Plant Biol. 2007 Oct;10(5):528-33
references.pmid: 17692561
references.citation: Genes Dev. 2002 Oct 15;16(20):2672-83
references.pmid: 12381666
references.citation: Plant Cell. 1998 Oct;10(10):1747-58
references.pmid: 9761800
references.citation: EMBO J. 1987 Dec 20;6(13):3901-7
references.pmid: 3327686
references.citation: Mol Plant. 2009 Jul;2(4):688-699
references.pmid: 19825649
references.citation: Annu Rev Genomics Hum Genet. 2008;9:233-57
references.pmid: 18767965
references.citation: Mol Plant. 2010 Mar;3(2):300-13
references.pmid: 20080814
references.citation: Plant Cell. 2008 Apr;20(4):827-42
references.pmid: 18398050
references.citation: Symp Soc Exp Biol. 1986;40:77-84
references.pmid: 3544311
references.citation: Science. 1984 Nov 16;226(4676):792-801
references.pmid: 15739260
references.citation: Plant J. 2007 Aug;51(3):526-36
references.pmid: 17559516
references.citation: Nature. 2007 May 24;447(7143):407-12
references.pmid: 17522673
references.citation: Nat Genet. 2003 Jan;33(1):102-6
references.pmid: 12483211
references.citation: Plant J. 2001 Nov;28(3):307-17
references.pmid: 11722773
references.citation: Nature. 2006 Aug 31;442(7106):1046-9
references.pmid: 16892047
references.citation: Mol Plant. 2009 Jul;2(4):578-588
references.pmid: 19825640
references.citation: Plant Cell. 2000 Jul;12(7):1165-78
references.pmid: 10899982