title: Selective determination of homocysteine at physiological pH using nanostructured film of aminothiadiazole modified electrode.
abstract: We report the selective determination of homocysteine (HCY) in the presence of one of the very important interferents, ascorbic acid (AA) using electropolymerized film of 2-amino-1,3,4-thiadiazole (ATD) modified glassy carbon electrode (GCE) at physiological pH for the first time. An atomic force microscopic image showed that the electropolymerized film of ATD (p-ATD) formed a spherical like structure with a thickness of 25 nm. This nanostructured film oxidized HCY at 0.55 V while bare GCE failed to oxidize it at physiological pH. Further, p-ATD modified electrode successfully separated the voltammetric signals of AA and HCY with a peak separation of 490 mV. The amperometric current was increased linearly from 100 to 1400 nM HCY and achieved the detection limit of 51 pM (S/N=3). The present modified electrode showed better recoveries for spiked HCY in human blood serum samples.
authors.affiliation: Department of Chemistry, Gandhigram Rural University, Gandhigram-624302, Dindigul, India.
authors.forename: Palraj
authors.initials: P
authors.lastname: Kalimuthu
authors.forename: S Abraham
authors.initials: SA
authors.lastname: John
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D005898:Glass / Q000737:chemistry; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013830:Thiadiazoles / Q000737:chemistry*
chemical_list: D013830:Thiadiazoles; D006710:Homocysteine; C010852:2-amino-1,3,4-thiadiazole; D002244:Carbon