title: Biotransformation of mulberroside A from Morus alba results in enhancement of tyrosinase inhibition.
abstract: Mulberroside A, a glycosylated stilbene, was isolated and identified from the ethanol extract of the roots of Morus alba. Oxyresveratrol, the aglycone of mulberroside A, was produced by enzymatic hydrolysis of mulberroside A using the commercial enzyme Pectinex. Mulberroside A and oxyresveratrol showed inhibitory activity against mushroom tyrosinase with an IC(50) of 53.6 and 0.49 microM, respectively. The tyrosinase inhibitory activity of oxyresveratrol was thus approximately 110-fold higher than that of mulberroside A. Inhibition kinetics showed mulberroside A to be a competitive inhibitor of mushroom tyrosinase with L-tyrosine and L-DOPA as substrate. Oxyresveratrol showed mixed inhibition and noncompetitive inhibition against L-tyrosine and L-DOPA, respectively, as substrate. The results indicate that the tyrosinase inhibitory activity of mulberroside A was greatly enhanced by the bioconversion process.
authors.affiliation: Department of Chemical Engineering and Biotechnology, Korea Polytechnic University, Shihung-si, Kyunggi-do 429-793, South Korea.
authors.forename: Jeong-Keun
authors.initials: JK
authors.lastname: Kim
authors.forename: Mijin
authors.initials: M
authors.lastname: Kim
authors.forename: Ssang-Goo
authors.initials: SG
authors.lastname: Cho
authors.forename: Myung-Kyoo
authors.initials: MK
authors.lastname: Kim
authors.forename: Suhng Wook
authors.initials: SW
authors.lastname: Kim
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lim
mesh_terms: D000363:Agaricales / Q000201:enzymology; D001711:Biotransformation; D004187:Disaccharides / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000378:metabolism* / Q000494:pharmacology; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D031625:Morus / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism; D013267:Stilbenes / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D004187:Disaccharides; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D013267:Stilbenes; C420606:mulberroside A; C034912:puag-haad; D014442:Monophenol Monooxygenase
references.citation: Biotechnol Bioeng. 2004 Sep 30;87(7):849-54
references.pmid: 15334411
references.citation: J Agric Food Chem. 2007 May 16;55(10):3913-9
references.pmid: 17439230
references.citation: Life Sci. 2006 May 1;78(23):2724-33
references.pmid: 16313926
references.citation: J Pharm Sci. 2006 Jun;95(6):1177-95
references.pmid: 16639719
references.citation: Planta Med. 2000 Feb;66(1):11-5
references.pmid: 10705726
references.citation: J Agric Food Chem. 1999 Oct;47(10):4121-5
references.pmid: 10552777
references.citation: Bioorg Med Chem Lett. 2006 Nov 1;16(21):5650-3
references.pmid: 16919455
references.citation: J Biol Chem. 2002 May 3;277(18):16340-4
references.pmid: 11864987
references.citation: Planta Med. 1996 Dec;62(6):559-61
references.pmid: 17252500
references.citation: J Pharm Pharmacol. 2003 Dec;55(12):1695-700
references.pmid: 14738598
references.citation: Pharmacol Rev. 2004 Mar;56(1):53-102
references.pmid: 15001663
references.citation: J Nat Med. 2009 Jul;63(3):254-60
references.pmid: 19219523
references.citation: Pigment Cell Res. 2007 Oct;20(5):394-401
references.pmid: 17850513
references.citation: Biochem Biophys Res Commun. 1998 Feb 24;243(3):801-3
references.pmid: 9500997
references.citation: Planta Med. 2009 Jun;75(8):792-6
references.pmid: 19288403
references.citation: Biol Pharm Bull. 2001 Apr;24(4):351-6
references.pmid: 11305594
references.citation: J Agric Food Chem. 2006 Nov 1;54(22):8425-31
references.pmid: 17061816
references.citation: J Nutr. 2006 Feb;136(2):404-8
references.pmid: 16424119
references.citation: Science. 1982 Sep 17;217(4565):1163-5
references.pmid: 6810464
references.citation: Nitric Oxide. 2003 Sep;9(2):64-76
references.pmid: 14623172
references.citation: Brain Res. 2004 Aug 13;1017(1-2):98-107
references.pmid: 15261105
references.citation: J Agric Food Chem. 2007 Apr 4;55(7):2604-10
references.pmid: 17335224
references.citation: N Biotechnol. 2009 Oct 31;26(3-4):122-30
references.pmid: 19643210
references.citation: Br J Nutr. 2009 Oct;102(7):976-84
references.pmid: 19393110
references.citation: Chem Pharm Bull (Tokyo). 2002 Apr;50(4):450-2
references.pmid: 11963989
references.citation: J Appl Microbiol. 2007 Sep;103(3):601-12
references.pmid: 17714393