[Rescue of dendritic cells malfunction and loading with HBV proteins in patients with chronic hepatitis B in vitro].
OBJECTIVE
To investigate dendritic cell (DC) malfunctions in patients with chronic hepatitis B (CHB) and try some means to restore the function in vitro.


METHODS
Twelve CHB patients and 10 healthy people were enrolled in the study. Phenotype analysis and allogeneic mixed lymphocyte reaction (AMLR) assay of DC from these subjects were made. Enzyme-linked ELISpot method for detecting IFN-gamma-producing CD8 (+) T cells were used to evaluate the efficacy of DC loaded in vitro with HBsAg or HBcAg.


RESULTS
DC from patients had a lower expression of co-stimulatory molecules and impaired AMLR capacity, but was restored partially by cytokine cocktail in vitro. Mature DC loaded with HBsAg or HBcAg showed a greater capacity for IFN-gamma-production than immature DC.


CONCLUSIONS
Malfunction of DC from CHB patients may be rescued by a cocktail of cytokines, and therapeutic DC vaccines loaded with HBV protein might be helpful to treat CHB patients.
Duan
Xue-zhang
XZ
Liver Failure Treatment and Research Center, 302nd Hospital of PLA, Beijing 100039, China.
Su
Hai-bin
HB
Chen
Jing
J
Zhang
Ai-min
AM
Hu
Jin-hua
JH
Wang
Hui-fen
HF
D000328:Adult; D016022:Case-Control Studies; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D005260:Female; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology*; D019694:Hepatitis B, Chronic / Q000276:immunology* / Q000821:virology; D006801:Humans; D008297:Male; D014764:Viral Proteins / Q000276:immunology*; D055815:Young Adult
D016207:Cytokines; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D014764:Viral Proteins