Oxidative stress is independently associated with non-alcoholic fatty liver disease (NAFLD) in subjects with and without type 2 diabetes.
OBJECTIVE
Our work is aimed at exploring the interrelationship of oxidative stress and insulin resistance in NAFLD subjects with and without type 2 diabetes in a population-based study.


METHODS
Subjects [n=200] were recruited from the Chennai Urban Rural Epidemiology Study. 1: Normal glucose tolerance (NGT) subjects without NAFLD; 2: NGT with NAFLD; 3: type 2 diabetic subjects [T2DM] without NAFLD and 4: T2DM with NAFLD. Thiobarbituric acid reactive substances (TBARS), protein carbonyl (PCC) and glutathione levels were measured by standard methods. Ultrasound of the liver was used to diagnose NAFLD.


RESULTS
TBARS and PCC levels were significantly elevated and GSH/GSSG ratio was significantly decreased in diabetic subjects with NAFLD compared to all other groups (p trend <0.001). Oxidative stress markers significantly associated with NAFLD even after adjusting for age, gender, BMI and glycemic status.


CONCLUSIONS
Increased oxidative stress is independently associated with NAFLD in Asian Indians without and with T2DM.
Narasimhan
Sandhya
S
Madras Diabetes Research Foundation, Gopalapuram, Chennai, India.
Gokulakrishnan
Kuppan
K
Sampathkumar
Rangasamy
R
Farooq
Syed
S
Ravikumar
Radhakrishnan
R
Mohan
Viswanathan
V
Balasubramanyam
Muthuswamy
M
D000328:Adult; D015415:Biomarkers / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications; D005234:Fatty Liver / Q000097:blood* / Q000150:complications; D005978:Glutathione / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008875:Middle Aged; D018384:Oxidative Stress; D050050:Protein Carbonylation; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D015415:Biomarkers; D007328:Insulin; D017392:Thiobarbituric Acid Reactive Substances; D005978:Glutathione