Molecular recognition of DNA-protein complexes: a straightforward method combining scanning force and fluorescence microscopy.
Combining scanning force and fluorescent microscopy allows simultaneous identification of labeled biomolecules and analysis of their nanometer level architectural arrangement. Fluorescent polystyrene nano-spheres were used as reliable objects for alignment of optical and topographic images. This allowed the precise localization of different fluorescence particles within complex molecular assemblies whose structure was mapped in nanometer detail topography. Our experiments reveal the versatility of this method for analysis of proteins and protein-DNA complexes.
Sanchez
Humberto
H
Department of Cell Biology and Genetics, Cancer Genomics Center, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands.
Kanaar
Roland
R
Wyman
Claire
C
D010582:Bacteriophage lambda / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D004279:DNA, Viral / Q000737:chemistry / Q000648:ultrastructure; D005456:Fluorescent Dyes; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D054118:Nanospheres / Q000648:ultrastructure; D011137:Polystyrenes; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D045663:Quantum Dots; D051135:Rad51 Recombinase / Q000737:chemistry / Q000648:ultrastructure
D004279:DNA, Viral; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D011137:Polystyrenes; D011506:Proteins; D004247:DNA; C495153:RAD51 protein, human; D051135:Rad51 Recombinase
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