Caffeic acid phenethyl ester suppresses the induction of eotaxin in human lung fibroblast cells.
BACKGROUND
Eotaxin, a CC chemokine, plays an important role in inflammation of airway allergic diseases. The authors investigated the activities of caffeic acid phenethyl ester (CAPE), the active component of propolis, in regulating eotaxin production in human lung fibroblast.


METHODS
The authors used human lung fibroblasts, CCD-11Lu cells, stimulated with interleukin-13 (IL-13) and tumor necrosis factor-alpha (TNF-alpha), to induce eotaxin secretion. The cells were treated with CAPE of different concentrations and pretreatment duration to check its inhibition in eotaxin production. Enzyme-linked immunosorbent assay (ELISA) was used to measure eotaxin secretion; electrophoretic mobility shift assay (EMSA) to check nuclear factor kappa B (NF-kappaB)-promoter binding; and Western blot to quantitate the cyplasmic inhibitor of NF-kappaB (IkappaB) and nuclear NF-kappaB p65.


RESULTS
CAPE inhibited the production of eotaxin in CCD-11Lu cells stimulated by IL-13 and TNF-alpha combination in a dose- and time-dependent manner. The authors also demonstrated CAPE to be able to inhibit NF-kappaB activation in CCD-11Lu cells.


CONCLUSIONS
The authors suggest that CAPE is a promising agent in controlling eosinophils influx in human airway.
Liao
Yu-Ru
YR
Division of Immunology, Rheumatology and Allergy, Department of Pediatrics, Taichung Veterans General Hospital, Taichung, 40705, Taiwan.
Hsu
Jen-Yuan
JY
Chu
Jao-Jia
JJ
Fu
Lin-Shien
LS
D002109:Caffeic Acids / Q000494:pharmacology*; D002478:Cells, Cultured; D054413:Chemokine CCL11 / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D018793:Interleukin-13 / Q000494:pharmacology; D008168:Lung / Q000166:cytology / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
C515456:CCL11 protein, human; D002109:Caffeic Acids; D054413:Chemokine CCL11; D018793:Interleukin-13; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol