Characterization of D6S2806 and D6S2879 short tandem repeat loci in HLA-DRB1 region in Iranian population.
Genomewide screen analysis has shown the close association of the human leukocyte antigen (HLA)-DRB1 region with susceptibility to multiple sclerosis and a number of autoimmune diseases. Using bioinformatics software, several potential short tandem repeat (STR) markers have been introduced in this region in the major histocompatibility complex data base (dbMHC). In this study, the identity and characteristics of two putative STR markers, D6S2879 and D6S2806, in this region were examined in Iranian population. The loci were genotyped in 85 individuals using polymerase chain reaction followed by polyacrylamide gel electrophoresis and sequencing. Analysis of the allelic frequency showed the presence of six and four alleles for D6S2806 and D6S2879, respectively. Analysis of deviations from Hardy-Weinberg equilibrium (HWE) showed that D6S2806 was in equilibrium (P > 0.05). However, the D6S2879 locus showed a significant deviation from HWE (P < 0.05). Therefore, the D6S2806 locus could be suggested as a marker for linkage analysis and disease-susceptibility investigations in the MHC-DRB1 gene region.
Vallian
S
S
Division of Genetics, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Islamic Republic of Iran. svallian@biol.ui.ac.ir
Tajadod
M
M
Hojati
Z
Z
D030541:Databases, Genetic; D005787:Gene Frequency; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007492:Iran; D018895:Microsatellite Repeats / Q000235:genetics*; D016415:Sequence Alignment
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains