title: Prevention of biofilm formation on titanium surfaces modified with conjugated molecules comprised of antimicrobial and titanium-binding peptides.
abstract: Specific binding of antimicrobial peptides to titanium (Ti) surfaces may serve to prevent biofilm formation, leading to a reduction in peri-implantitis. This study evaluated the binding behavior of conjugated molecules consisting of antimicrobial and hexapeptidic Ti-binding peptides (minTBP-1) using the quartz crystal microbalance (QCM-D) technique, and investigated the effect of modification of Ti surfaces with these peptides on the bioactivity of Porphyromonas gingivalis. Four kinds of peptide were prepared: histatin 5 (DSHAKRHHGYKRKFHEKHHSHRGY), minTBP-1 + histatin 5 (RKLPDAPDSHAKRHHGYKRKFHEKHHSHRGY), lactoferricin (FQWQRNMRKVR), and minTBP-1 + lactoferricin (RKLPDAPGGFQWQRNMRKVR). The QCM-D analysis demonstrated that significantly larger increases in peptide adsorption were observed in the conjugated peptides than in antimicrobial peptides alone. In addition, ATP activity in P. gingivalis in peptide-modified specimens significantly decreased compared to that in the Ti control. These results indicate that surface modification with conjugated molecules consisting of antimicrobial and Ti-binding peptides is a promising method for reduction of biofilm formation on Ti surfaces.
authors.affiliation: Division of Oral Implants Research and HRC7, Oral Health Science Center, Tokyo Dental College, Mihama-ku, Chiba, Japan. yosinari@tdc.ac.jp
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshinari
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kato
authors.forename: Kenichi
authors.initials: K
authors.lastname: Matsuzaka
authors.forename: Tohru
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Shiba
mesh_terms: D000327:Adsorption; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D015921:Dental Implants / Q000382:microbiology*; D008855:Microscopy, Electron, Scanning; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D056951:Photoelectron Spectroscopy; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000254:growth & development; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D010455:Peptides; D014025:Titanium