title: [Molecular cloning and sequence analysis of lactate dehydrogenase gene from Plasmodium vivax Hainan isolate].
abstract: Full length sequence of lactate dehydrogenase gene was amplified by PCR from the genomic DNA of Plasmodium vivax Hainan isolate, and named as PvLDH/HN (GenBank No. FJ527750). Sequence analysis showed that the gene had 951 bp, coding 316 aa. Compared with PvLDH/Salvador and PvLDH/Belem, the nucleotide sequence homology of PvLDH/HN was both 99.89%, while the homology of amino acid sequence was 100%. Topology analysis showed that the protein had two transmembrane alpha-helices, which suggested that the protein might be a membrane protein. The major antigen epitope regions (82-95aa) was presented on the protein surface, and formed the specific substrate binding loop, which revealed that it might be an ideal target site for drug action and immunodiagnosis.
authors.affiliation: Department of Parasitology, Hainan Medical College, Haikou 571101, China. luganghn@yahoo.com.cn
authors.forename: Gang
authors.initials: G
authors.lastname: Lv
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Li
authors.forename: Shi-yong
authors.initials: SY
authors.lastname: Zhao
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Lu
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Fan
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000201:enzymology* / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D007770:L-Lactate Dehydrogenase