title: Multivariate analysis of differential lymphocyte cell cycle activity in Alzheimer's disease.
abstract: Mounting evidence suggests cell cycle dysregulation is involved in the pathogenesis of Alzheimer's disease (AD) and that this failure is systemic, affecting not only neurons but also peripheral blood lymphocytes (PBLs). This study analyzed if differences in PBL proliferation activity could be used as a diagnostic biomarker for AD. CD69 and CD28 expressions on PBL T, B, and monocyte cells were measured by flow cytometry with and without mitogenic stimulation in healthy controls (HC), probable AD, and Parkinson's disease dementia (PDD) subjects. Univariate and multivariate scoring models were employed to evaluate the data relative to the clinical diagnoses. Eleven CD expression markers were significantly altered in AD subjects compared with a mixed pool of PDD and HC subjects using univariate models. Using multivariate models, seven CD expression markers were significantly altered in AD subjects compared with PDD subjects. Multivariate scoring demonstrated up to a 91% positive and 92% negative agreement with subject clinical diagnosis and had little correlation with the severity of dementia. Present findings suggest that with further development this analytical and multivariate modeling procedure could aid the current differential diagnosis of Alzheimer's disease.
authors.affiliation: Paul Flechsig Institute for Brain Research, University of Leipzig, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Stieler
authors.forename: Randy
authors.initials: R
authors.lastname: Grimes
authors.forename: Don
authors.initials: D
authors.lastname: Weber
authors.forename: William
authors.initials: W
authors.lastname: Gartner
authors.forename: Marwan
authors.initials: M
authors.lastname: Sabbagh
authors.forename: Thomas
authors.initials: T
authors.lastname: Arendt
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D002453:Cell Cycle / Q000276:immunology*; D018797:Cell Cycle Proteins / Q000276:immunology*; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology*; D008297:Male; D015999:Multivariate Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D018797:Cell Cycle Proteins
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