Whole cell protein profiling reiterate phylogenetic relationships among strains of Yersinia enterocolitica biovar 1A as discerned earlier by different genotyping methods.
OBJECTIVE
To evaluate whole cell protein profiling vis-à-vis genotyping to discern phylogenetic relationships among strains of Y. enterocolitica biovar 1A.


RESULTS
Whole cell protein profiling of Y. enterocolitica biovar 1A strains was performed using SDS-PAGE. Twenty-one distinct protein profile types were obtained among a collection of 81 strains isolated from clinical and nonclinical sources. Whole cell protein profiling exhibited discriminatory index (DI) of 0·80 and clustered the strains into two distinct clonal groups. The clinical and the aquatic serotype O:6,30-6,31 strains were clustered into two discrete subgroups.


CONCLUSIONS
Whole cell protein profiling displayed sufficient diversity among strains of Y. enterocolitica biovar 1A, and the phylogenetic relationships obtained were in good agreement with those established earlier by genotyping techniques.


CONCLUSIONS
Whole cell protein profiling was as discriminatory as some of the genotyping methods and has the potentiality to be used as an adjunct tool to study epidemiology of Y. enterocolitica.
Mallik
S
S
Microbial Pathogenicity Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, India.
Virdi
J S
JS
D001426:Bacterial Proteins / Q000032:analysis*; D015373:Bacterial Typing Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D005838:Genotype; D010802:Phylogeny; D015008:Yersinia enterocolitica / Q000737:chemistry / Q000145:classification* / Q000235:genetics
D001426:Bacterial Proteins