title: Optimized speckle variance OCT imaging of microvasculature.
abstract: We optimize speckle variance optical coherence tomography (svOCT) imaging of microvasculature in high and low bulk tissue motion scenarios. To achieve a significant level of image contrast, frame rates must be optimized such that tissue displacement between frames is less than the beam radius. We demonstrate that higher accuracy estimates of speckle variance can enhance the detection of capillaries. These findings are illustrated in vivo by imaging the dorsal window chamber model (low bulk motion). We also show svOCT imaging of the nonstabilized finger (high bulk motion), using optimized imaging parameters, demonstrating better vessel detection than Doppler OCT.
authors.affiliation: Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Adrian
authors.initials: A
authors.lastname: Mariampillai
authors.forename: Michael K K
authors.initials: MK
authors.lastname: Leung
authors.forename: Mark
authors.initials: M
authors.lastname: Jarvi
authors.forename: Beau A
authors.initials: BA
authors.lastname: Standish
authors.forename: Kenneth
authors.initials: K
authors.lastname: Lee
authors.forename: Brian C
authors.initials: BC
authors.lastname: Wilson
authors.forename: Alex
authors.initials: A
authors.lastname: Vitkin
authors.forename: Victor X D
authors.initials: VX
authors.lastname: Yang
mesh_terms: D018316:Gliosarcoma / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D055806:Microvessels / Q000166:cytology* / Q000473:pathology / Q000502:physiology / Q000503:physiopathology; D009068:Movement; D009262:Nails / Q000098:blood supply; D019047:Phantoms, Imaging; D041623:Tomography, Optical Coherence / Q000379:methods*