title: Levels of follicular G-CSF and interleukin-15 appear as noninvasive biomarkers of subsequent successful birth in modified natural in vitro fertilization/intracytoplasmic sperm injection cycles.
abstract: OBJECTIVE
  To explore oocyte competence for subsequent birth. The modified natural IVF/intracytoplasmic sperm injection (ICSI) cycle was used as an experimental model by measuring levels of cytokines, chemokines, and growth factors in individual follicular fluids (FF).
  
  
  METHODS
  A retrospective blinded study.
  
  
  METHODS
  European network of research, Embryo Implantation Control (EMBIC).
  
  
  METHODS
  Single FF from 83 women were analyzed during a modified natural IVF/ICSI cycle, and reproducibility of follicular composition was evaluated over two cycles for 15 patients.
  
  
  METHODS
  Each FF sample was blindly tested to assess levels of 26 factors by bead-based immunoassays.
  
  
  METHODS
  Each mediator was evaluated as a potential biomarker of subsequent birth by multivariate regression analysis.
  
  
  RESULTS
  A combination of both FF G-CSF and IL-15 was the optimal model to predict birth (AUC(ROC), 0.85). Birth rates per cycle were 48.9% (16/33) if two good-prognosis criteria were present (FF G-CSF>12 pg/mL and IL-15<7 pg/mL) and 8% (3/36) and 0% (0/14) if, respectively, one or none were present. FF G-CSF was significantly correlated over two cycles (r=.71), suggesting a possible prognostic value of its documentation.
  
  
  CONCLUSIONS
  Combined follicular G-CSF and IL-15 quantification appears as an efficient and noninvasive method to define oocyte competence for subsequent successful conception in modified natural IVF/ICSI cycles.
authors.affiliation: INSERM U782, University Paris-Sud, and AP-HP, Service d’histologie, embryologie, cytogenetique, HôpitalAntoine Béclère,Clamart, France. nathalie-ledee@orange.fr
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lédée
authors.forename: René
authors.initials: R
authors.lastname: Frydman
authors.forename: Agnessia
authors.initials: A
authors.lastname: Osipova
authors.forename: Joelle
authors.initials: J
authors.lastname: Taieb
authors.forename: Vanessa
authors.initials: V
authors.lastname: Gallot
authors.forename: Letizia
authors.initials: L
authors.lastname: Lombardelli
authors.forename: Federica
authors.initials: F
authors.lastname: Logiodice
authors.forename: Marie
authors.initials: M
authors.lastname: Petitbarat
authors.forename: Renato
authors.initials: R
authors.lastname: Fanchin
authors.forename: Gérard
authors.initials: G
authors.lastname: Chaouat
authors.forename: Nelly
authors.initials: N
authors.lastname: Achour-Frydman
authors.forename: Marie Pierre
authors.initials: MP
authors.lastname: Piccinni
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D019409:Interleukin-15 / Q000378:metabolism*; D015999:Multivariate Analysis; D006080:Ovarian Follicle / Q000378:metabolism; D011237:Predictive Value of Tests; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D011379:Prognosis; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D015415:Biomarkers; D019409:Interleukin-15; D016179:Granulocyte Colony-Stimulating Factor