Cell-trappable fluorescent probes for nitric oxide visualization in living cells.
Two new cell-trappable fluorescent probes for nitric oxide (NO) are reported based on either incorporation of hydrolyzable esters or conjugation to aminodextran polymers. Both probes are highly selective for NO over other reactive oxygen and nitrogen species (RONS). The efficacy of these probes for the fluorescence imaging of nitric oxide produced endogenously in Raw 264.7 cells is demonstrated.
Pluth
Michael D
MD
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
McQuade
Lindsey E
LE
Lippard
Stephen J
SJ
D000818:Animals; D002460:Cell Line; D003300:Copper / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D004952:Esters / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013237:Stereoisomerism
D003911:Dextrans; D004952:Esters; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D011108:Polymers; D009569:Nitric Oxide; D003300:Copper
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