Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration.
Cell migration is important in several biological phenomena, such as cancer metastasis. Therefore, the identification of genes involved in cell migration might facilitate the discovery of antimetastatic drugs. However, screening of genes by the current methods can be complicated by factors related to cell stimulation, for example, abolition of contact inhibition and the release inflammatory cytokines from wounded cells during examinations of wound healing in vitro. To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening.
Onuki-Nagasaki
Reiko
R
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan.
Nagasaki
Akira
A
Hakamada
Kazumi
K
Uyeda
Taro Q P
TQ
Fujita
Satoshi
S
Miyake
Masato
M
Miyake
Jun
J
D001681:Biological Assay; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D024042:Collagen Type I / Q000494:pharmacology; D005353:Fibronectins / Q000378:metabolism; D006367:HeLa Cells; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010641:Phenotype; D012235:Rhodamines / Q000378:metabolism; D013223:Statistics as Topic; D014162:Transfection / Q000379:methods*
D024042:Collagen Type I; D005353:Fibronectins; D012235:Rhodamines