Effects of ovariectomy and resistance training on MMP-2 activity in rat calcaneal tendon.
Tendon remodeling relies on extracellular matrix (ECM) restructuring by the matrix metallopeptidases (MMPs). The aim of this study was to investigate MMP-2 activity in different regions of the calcaneal tendon (CT) after resistance training (RT) in ovariectomized rats. Wistar adult female rats were grouped into sedentary (Sed-Intact), ovariectomized sedentary (Sed-Ovx), acute exercise (AcuteEx-Intact), ovariectomized acute exercise (AcuteEx-Ovx), resistance trained (ChronicEx-Intact), and ovariectomized resistance trained (ChronicEx-Ovx) (n = 10 each group). The RT protocol required the animals to climb a 1.1-m vertical ladder with weights attached to their tail. The sessions were performed once every 3 days with 4-9 climbs and 8-12 dynamic movements per scaling. The acute groups performed one session and the chronic groups underwent 12 weeks of RT. There was an increase in total MMP-2 activity in Sed-Ovx, AcuteEx-Intact, and ChronicEx-Intact compared with that in Sed-Intact in the proximal region of CT. AcuteEx-Ovx exhibited higher total MMP-2 than Sed-Ovx and AcuteEx-Intact in the distal region of CT. Chronic-Ovx presented lower total MMP-2 activity than Sed-Ovx and Chronic-Intact in the distal region of tendon. The active MMP-2 was higher for the AcuteEx-Ovx than Sed-Ovx and AcuteEx-Intact in proximal region of tendon. There was higher active MMP-2 in the distal region of tendon in the Acute-Ovx than in the Sed-Ovx and AcuteEx-Intact. Ovariectomy and resistance exercise modulate MMP-2 activity according to specific tendon region, indicating a differentiated tissue remodeling.
Pereira
Guilherme Borges
GB
Department of Physiological Sciences, Federal University of São Carlos, São Carlos/SP, Brazil. guifisiologia@gmail.com
Prestes
Jonato
J
Leite
Richard Diego
RD
Magosso
Rodrigo Ferro
RF
Peixoto
Fabiana Sobral
FS
Marqueti
Rita de Cássia
Rde C
Shiguemoto
Gilberto Eiji
GE
Selistre-de-Araújo
Heloisa Sobreiro
HS
Baldissera
Vilmar
V
Perez
Sérgio Eduardo de Andrade
SE
D000125:Achilles Tendon / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D002111:Calcaneus / Q000166:cytology / Q000201:enzymology; D004789:Enzyme Activation / Q000502:physiology; D005109:Extracellular Matrix / Q000201:enzymology; D005260:Female; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D010052:Ovariectomy / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology; D055070:Resistance Training / Q000379:methods*
D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat