Cell screening using disposable photonic lab on a chip systems.
A low-cost photonic lab on a chip with three different working regimes for cell screening is presented. The proposed system is able to perform scattering, scattering + absorption, and absorption measurements without any modification. Opposite to the standard flow cytometers, in this proposed configuration, a single 30 ms scan allows to obtain information regarding the cell optical properties. An additional novelty is that the whole spectrum is obtained and analyzed, being then possible to determine for each regime which is the optimal working wavelength that would provide the best performance in terms of sensitivity and limit of detection (LOD). Experimental results have provided with an LOD of 54.9 +/- 0.7 cells (in the scattering regime using unlabeled cells), 53 +/- 1 cells (in the scattering + absorption regime using labeled cells), and 105 +/- 4 cells (in the absorption regime using labeled cells). Finally, the system has also been used for measuring the dead/live cell ratio, obtaining LODs between 7.6 +/- 0.4% and 6.7 +/- 0.3%, depending on the working regime used.
Ibarlucea
Bergoi
B
Centre Nacional de Microelectrònica (IMB-CNM, CSIC), Barcelona, Spain.
Fernandez-Rosas
Elisabet
E
Vila-Planas
Jordi
J
Demming
Stefanie
S
Nogues
Carme
C
Plaza
Jose A
JA
Büttgenbach
Stephanus
S
Llobera
Andreu
A
D000042:Absorption; D002477:Cells / Q000737:chemistry*; D022082:Cellular Structures; D057230:Limit of Detection; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D017785:Photons