Interaction of common sequence variants and selected risk factors in determination of HDL cholesterol levels.
OBJECTIVE
The aim of our study was to assess the association of common sequence variants, and selected interactions, with HDL-c plasma levels.


METHODS
We analysed 743 individuals (340 men and 403 women) with high mean triglyceride and LDL-c levels. The association of five polymorphic sites (ABCA1 g.1051G>A, APOA1 g.-75G>A, CETP g.-629C>A, HNF1A g.102A>C, and LIPG g.584C>T), apoE isoforms and selected interactions with HDL-c levels were evaluated using linear regression models.


RESULTS
After adjusting for triglycerides, sex, and BMI the only genotype with a statistically significant effect on HDL-c levels (p-value=0.004) was the CETP promoter variant. Further, linear regression model with interactions included indicated possible interplay between APOA1 genotype and menopause (p-value=0.002) and ABCA1 and APOE isoforms (p-value=0.017) on HDL-c plasma concentration.


CONCLUSIONS
Our study indicated that not only the CETP variant but also apoE isoforms and menopause could operate as potent modulators of HDL-c concentrations.
Katerina
Hirschfeldova
H
Institute of Biology and Medical Genetics, 1st Faculty of Medicine and General Teaching Hospital, Charles University in Prague, 12800 Prague, Czech Republic. kzidk@lf1.cuni.cz
Michaela
Sedova
S
Michal
Vrablik
V
Helena
Svobodova
S
Jana
Zvarova
Z
Jaroslav
Hubacek
H
Richard
Ceska
C
D000328:Adult; D001057:Apolipoproteins E; D053480:Cholesterol Ester Transfer Proteins / Q000235:genetics*; D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008593:Menopause; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D020033:Protein Isoforms; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood
D001057:Apolipoproteins E; C506642:CETP protein, human; D053480:Cholesterol Ester Transfer Proteins; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D020033:Protein Isoforms; D014280:Triglycerides