title: Isolation and identification of two new fungal strains for xylanase production.
abstract: Fungi are well known for their ability to excrete enzymes into the environment. The aim of this work was to evaluate xylanase production by fungi isolated from soil. One hundred and thirty-six fungal isolates were screened for xylanase production. Two xylanase producing isolates, FSS117 and FSS129, were identified on the basis of analyses of 5,8S gene sequencing. The closest phylogenetic neighbors according to 5,8S gene sequence data for the two isolates were Aspergillus tubingensis and Aspergillus terreus, respectively. When birchwood xylan or corn cob hulls was used as a substrate for 5 days under submerged culture cultivation, xylanase production from A. terreus FSS129 was 113 and 174 IU ml(-1), respectively. The pH and temperature for optimum xylanase activity were 8 and 65 degrees C.
authors.affiliation: Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, Syria. scientific1@aec.org.sy
authors.forename: Yasser
authors.initials: Y
authors.lastname: Bakri
authors.forename: Magali
authors.initials: M
authors.lastname: Masson
authors.forename: Philippe
authors.initials: P
authors.lastname: Thonart
mesh_terms: D001230:Aspergillus / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal; D004275:DNA, Ribosomal / Q000235:genetics; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012988:Soil Microbiology
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D005656:Fungal Proteins; D043364:Endo-1,4-beta Xylanases