title: A reversible, non-invasive method for airway resistance measurements and bronchoalveolar lavage fluid sampling in mice.
abstract: Airway hyperreactivity (AHR) measurements and bronchoalveolar lavage (BAL) fluid sampling are essential to experimental asthma models, but repeated procedures to obtain such measurements in the same animal are generally not feasible. Here, we demonstrate protocols for obtaining from mice repeated measurements of AHR and bronchoalveolar lavage fluid samples. Mice were challenged intranasally seven times over 14 days with a potent allergen or sham treated. Prior to the initial challenge, and within 24 hours following each intranasal challenge, the same animals were anesthetized, orally intubated and mechanically ventilated. AHR, assessed by comparing dose response curves of respiratory system resistance (RRS) induced by increasing intravenous doses of acetylcholine (Ach) chloride between sham and allergen-challenged animals, were determined. Afterwards, and via the same intubation, the left lung was lavaged so that differential enumeration of airway cells could be performed. These studies reveal that repeated measurements of AHR and BAL fluid collection are possible from the same animals and that maximal airway hyperresponsiveness and airway eosinophilia are achieved within 7-10 days of initiating allergen challenge. This novel technique significantly reduces the number of mice required for longitudinal experimentation and is applicable to diverse rodent species, disease models and airway physiology instruments.
authors.affiliation: Department of Medicine, Baylor College of Medicine, USA.
authors.forename: Sumanth
authors.initials: S
authors.lastname: Polikepahad
authors.forename: Wade T
authors.initials: WT
authors.lastname: Barranco
authors.forename: Paul
authors.initials: P
authors.lastname: Porter
authors.forename: Bruce
authors.initials: B
authors.lastname: Anderson
authors.forename: Farrah
authors.initials: F
authors.lastname: Kheradmand
authors.forename: David B
authors.initials: DB
authors.lastname: Corry
mesh_terms: D000485:Allergens / Q000008:administration & dosage; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000175:diagnosis* / Q000473:pathology / Q000503:physiopathology; D001985:Bronchial Provocation Tests / Q000379:methods; D018893:Bronchoalveolar Lavage / Q000379:methods*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology*; D004195:Disease Models, Animal; D051379:Mice; D008810:Mice, Inbred C57BL; D012137:Respiratory System / Q000503:physiopathology
chemical_list: D000485:Allergens
references.citation: Am J Respir Crit Care Med. 1997 Sep;156(3 Pt 1):766-75
references.pmid: 9309991
references.citation: Mol Med. 1998 May;4(5):344-55
references.pmid: 9642684
references.citation: Science. 1998 Dec 18;282(5397):2261-3
references.pmid: 9856950
references.citation: Am J Physiol. 1958 Feb;192(2):364-8
references.pmid: 13508884
references.citation: J Appl Physiol (1985). 2002 Oct;93(4):1198-207
references.pmid: 12235015
references.citation: Am J Respir Cell Mol Biol. 2004 Sep;31(3):373-4
references.pmid: 15317683
references.citation: J Exp Med. 1996 Jan 1;183(1):109-17
references.pmid: 8551213
references.citation: J Appl Physiol (1985). 2004 Jul;97(1):286-92
references.pmid: 15033965