title: Magnetic resonance spectroscopy of live Drosophila melanogaster using magic angle spinning.
abstract: High-Resolution Magic Angle Spinning (HRMAS) proton magnetic resonance spectroscopy ((1)H-MRS) is a novel non-destructive technique that improves spectral line-widths and allows high-resolution spectra to be obtained from extracts, intact cells, cell cultures, and more importantly intact tissue to investigate relationships between metabolites and cellular processes. In vivo HRMAS (1)H-MRS studies have yet to be reported in the live fruit fly Drosophila melanogaster. Drosophila, as a simpler genetic organism, allows the multiple biological functions and various evolutionarily conserved signaling pathways to be examined at the whole organism level and it is a useful model for investigating genetics and physiology. To this end, we developed and implemented an in vivo HRMAS (1)H-MRS method to investigate live Drosophila at 14.1 T. Here, we outline an HRMAS (1)H-MRS protocol for the molecular characterization of Drosophila with a conventional MR spectrometer equipped with an HRMAS probe. This technique is a novel, in vivo, non-destructive Drosophila metabolite measurement approach, which enables the identification of disease biomarkers and thus may contribute to novel therapeutic development.
authors.affiliation: NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, MA, USA.
authors.forename: Valeria
authors.initials: V
authors.lastname: Righi
authors.forename: Yiorgos
authors.initials: Y
authors.lastname: Apidianakis
authors.forename: Laurence G
authors.initials: LG
authors.lastname: Rahme
authors.forename: A Aria
authors.initials: AA
authors.lastname: Tzika
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*
references.citation: Nat Protoc. 2009;4(9):1285-94
references.pmid: 19680242
references.citation: Magn Reson Med. 2005 Jan;53(1):41-8
references.pmid: 15690501
references.citation: J Magn Reson. 2008 Aug;193(2):251-8
references.pmid: 18556227
references.citation: PLoS One. 2008 Jul 30;3(7):e2817
references.pmid: 18665264
references.citation: FASEB J. 2005 Sep;19(11):1431-40
references.pmid: 16126910