title: Candida albicans SH3-domain proteins involved in hyphal growth, cytokinesis, and vacuolar morphology.
abstract: This report describes the analyses of three Candida albicans genes that encode Src Homology 3 (SH3)-domain proteins. Homologs in Saccharomyces cerevisiae are encoded by the SLA1, NBP2, and CYK3 genes. Deletion of CYK3 in C. albicans was not feasible, suggesting it is essential. Promoter shutdown experiments of CaCYK3 revealed cytokinesis defects, which are in line with the localization of GFP-tagged Cyk3 at septal sites. Deletion of SLA1 resulted in strains with decreased ability to form hyphal filaments. The number of cortical actin patches was strongly reduced in Deltasla1 strains during all growth stages. Sla1-GFP localizes in patches that are found concentrated at the hyphal tip. Deletion of the first two SH3-domains of Sla1 still resulted in cortical localization of the truncated protein. However, the actin cytoskeleton in this strain was aberrant like in the Deltasla1 deletion mutant indicating a function of these SH3 domains to recruit actin nucleation to sites of endocytosis. Deletion of NBP2 resulted in a defect in vacuolar fusion in hyphae. Germ cells of Deltanbp2 strains lacked a large vacuole but initiated several germ tubes. The mutant phenotypes of Deltanbp2 and Deltasla1 could be corrected by reintegration of the wild-type genes.
authors.affiliation: Carlsberg Laboratory, Yeast Biology, Valby, Copenhagen, Denmark.
authors.forename: Patrick
authors.initials: P
authors.lastname: Reijnst
authors.forename: Sigyn
authors.initials: S
authors.lastname: Jorde
authors.forename: Jürgen
authors.initials: J
authors.lastname: Wendland
mesh_terms: D002176:Candida albicans / Q000737:chemistry*; D048749:Cytokinesis / Q000235:genetics; D005656:Fungal Proteins / Q000502:physiology*; D025301:Hyphae / Q000235:genetics / Q000254:growth & development; D014617:Vacuoles / Q000235:genetics / Q000648:ultrastructure; D018909:src Homology Domains / Q000502:physiology*
chemical_list: D005656:Fungal Proteins
references.citation: J Cell Sci. 2000 Feb;113 ( Pt 3):365-75
references.pmid: 10639324
references.citation: Genetics. 2003 Oct;165(2):517-29
references.pmid: 14573466
references.citation: J Cell Sci. 2000 Feb;113 ( Pt 4):571-85
references.pmid: 10652251
references.citation: Curr Biol. 1997 Dec 1;7(12):921-9
references.pmid: 9382845
references.citation: J Cell Sci. 2004 Oct 1;117(Pt 21):4947-58
references.pmid: 15367585
references.citation: Eukaryot Cell. 2005 Feb;4(2):298-309
references.pmid: 15701792
references.citation: Science. 1999 Aug 6;285(5429):901-6
references.pmid: 10436161
references.citation: Mol Microbiol. 2002 Oct;46(1):269-80
references.pmid: 12366849
references.citation: EMBO Rep. 2006 Feb;7(2):186-91
references.pmid: 16374509
references.citation: EMBO J. 2007 Aug 22;26(16):3760-9
references.pmid: 17673907
references.citation: Eukaryot Cell. 2004 Apr;3(2):471-82
references.pmid: 15075276
references.citation: J Cell Sci. 2001 Apr;114(Pt 7):1253-63
references.pmid: 11256992
references.citation: Cell Microbiol. 2005 Nov;7(11):1546-54
references.pmid: 16207242
references.citation: Mol Biol Cell. 2007 Jan;18(1):265-81
references.pmid: 17093060
references.citation: Proteins. 2009 Oct;77(1):139-58
references.pmid: 19408297
references.citation: Eukaryot Cell. 2003 Feb;2(1):9-18
references.pmid: 12582118
references.citation: Mol Cell Biol. 2000 Jan;20(1):12-25
references.pmid: 10594004
references.citation: J Cell Biol. 1987 Oct;105(4):1539-47
references.pmid: 2444598
references.citation: J Am Acad Dermatol. 1994 Sep;31(3 Pt 2):S2-5
references.pmid: 8077502
references.citation: Eukaryot Cell. 2006 Feb;5(2):359-67
references.pmid: 16467476
references.citation: Methods Mol Biol. 2009;499:175-94
references.pmid: 19152049
references.citation: Trends Microbiol. 2001 Jul;9(7):327-35
references.pmid: 11435107
references.citation: Eukaryot Cell. 2007 Oct;6(10):1736-44
references.pmid: 17766464
references.citation: J Cell Sci. 2002 Apr 15;115(Pt 8):1703-15
references.pmid: 11950888
references.citation: Mol Microbiol. 2009 Jan;71(2):505-19
references.pmid: 19040629
references.citation: Curr Genet. 2003 Mar;42(6):339-43
references.pmid: 12612807
references.citation: Mol Microbiol. 1999 Nov;34(4):792-8
references.pmid: 10564518
references.citation: FEMS Yeast Res. 2008 Dec;8(8):1339-48
references.pmid: 19054134
references.citation: Curr Biol. 2000 Jul 27-Aug 10;10(15):947-50
references.pmid: 10959846
references.citation: Yeast. 2007 Jun;24(6):511-22
references.pmid: 17431925
references.citation: Eukaryot Cell. 2002 Apr;1(2):213-28
references.pmid: 12455956
references.citation: Trends Microbiol. 2004 Jul;12(7):317-24
references.pmid: 15223059
references.citation: Fungal Genet Biol. 2002 Oct;37(1):81-8
references.pmid: 12223192
references.citation: Yeast. 2003 Dec;20(16):1339-47
references.pmid: 14663826
references.citation: Nat Protoc. 2008;3(9):1414-21
references.pmid: 18772868
references.citation: Nat Rev Genet. 2002 Dec;3(12):918-30
references.pmid: 12459722
references.citation: Mol Gen Genet. 1984;198(2):179-82
references.pmid: 6394964
references.citation: J Basic Microbiol. 2006;46(5):416-29
references.pmid: 17009297
references.citation: Fungal Genet Biol. 2001 Nov;34(2):63-82
references.pmid: 11686673
references.citation: J Bacteriol. 2000 Oct;182(20):5730-6
references.pmid: 11004171