title: Cobalt-induced changes in the IGF-I and progesterone release, expression of proliferation- and apoptosis-related peptides in porcine ovarian granulosa cells in vitro.
abstract: Cobalt (Co) is an essential element. The general objective of this in vitro study was to examine dose-dependent changes in the secretory activity of porcine ovarian granulosa cells after experimental Co administration and to outline the potential intracellular mediators of its effects. Concentrations of IGF-I and progesterone were determined by RIA and expression of cyclin B1 and caspase-3 by immunocytochemistry. IGF-I release by granulosa cells was stopped by Co addition at the concentration 1 mg/mL. Progesterone release by granulosa cells was decreased at the lowest Co addition (0.09 mg/mL). In our study the changes of the expression of proliferation related peptide cyclin B1 and apoptosis related peptide caspase-3 in ovarian granulosa cells was observed after experimental Co addition. The molecular pathways stimulated by Co through the expression of cyclin B1 and caspase-3 were found. In conclusion, this study provides novel evidence that cobalt is the factor which can initiate adverse effects in ovarian granulosa cells. These results contribute towards the understanding of mechanisms relating to endocrine disruptor-induced alterations in porcine ovarian granulosa cells.
authors.affiliation: Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic. Adriana.Kolesarova@uniag.sk
authors.forename: Adriana
authors.initials: A
authors.lastname: Kolesarova
authors.forename: Marcela
authors.initials: M
authors.lastname: Capcarova
authors.forename: Alexander
authors.initials: A
authors.lastname: Sirotkin
authors.forename: Marina
authors.initials: M
authors.lastname: Medvedova
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Kovacik
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003035:Cobalt / Q000633:toxicity*; D056744:Cyclin B1 / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D013552:Swine
chemical_list: D056744:Cyclin B1; D003035:Cobalt; D011374:Progesterone; D007334:Insulin-Like Growth Factor I