[Effects of space flight on protein content and electrophoresis in Glycyrrhiza uralensis].
OBJECTIVE
To investigate the space environment on the role of licorice mutagenesis analysis of proteins.


METHODS
Licorice (Glycyrrhiza uralensis) seeds were carried by a recoverable satellite for 18 days (the average radiation dose in the flight recovery module was 0.102 m x d(-1), the orbit semidiameter 350 km, gravity 10(-6)). After return, The satellite-flown seeds and the unflown seeds (ground control) were planted in the fields of experimental farm. The leaves of each group were used for studying the effects of space flight on CAT, SOD activity, the protein content and electrophoresis.


RESULTS
After the space flight, CAT, SOD activity of licorice increased in varying degrees, the difference was significant (P<0.05), two types of enzyme activity of sample from Ordos were higher than that from Hangjinqi. The protein content of licorice increased in a certain extent, the difference was significant (P<0.05), while protein electrophoresis also showed differences, weak new bands appeared.


CONCLUSIONS
These results indicated that spaceflight has effect on protein of licorice, these changes may be used as a tool for accelerating the progress in G. uralensis breeding.
Yan
Shuo
S
College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072, China.
Gao
Wenyuan
W
Lu
Fuping
F
Zhao
Runhuai
R
D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis* / Q000378:metabolism; D004586:Electrophoresis; D005118:Extraterrestrial Environment; D029882:Glycyrrhiza uralensis / Q000737:chemistry* / Q000201:enzymology; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D018531:Spacecraft; D013482:Superoxide Dismutase / Q000032:analysis* / Q000378:metabolism
D010940:Plant Proteins; D013482:Superoxide Dismutase; D015500:Chloramphenicol O-Acetyltransferase