Bienzyme bionanomultilayer electrode for glucose biosensing based on functional carbon nanotubes and sugar-lectin biospecific interaction.
Bienzyme bionanomultilayer electrode for glucose biosensing was constructed based on functional carbon nanotubes and sugar-lectin biospecific interaction through layer-by-layer (LBL) assembly. After being functionalized by wrapping with polyelectrolyte, multiwalled carbon nanotubes (MCNTs) were water soluble and positively charged. MCNT-bienzyme bionanomultilayer electrode was then fabricated by LBL assembly of horseradish peroxidase (HRP) and glucose oxidase (GOD) on functional MCNT modified electrode. The attachment of the MCNT-bienzyme bionanomultilayer with the underlying electrode and each layer in the bionanomultilayer was based on reliably electrostatic or sugar-lectin biospecific interaction. The developed bienzyme biosensor exhibited fast amperometric response for the determination of glucose. The linear response of the developed biosensor for the determination of glucose ranged from 2.0 x 10(-6) to 1.7 x 10(-4) M with a detection limit of 2.5 x 10(-7) M. The biosensor can be used directly to determine glucose in serum. The construction of the bienzyme biosensor showed potential for the preparation of MCNT-enzyme nanocomposite with controllability and high performance.
Chen
Huan
H
Department of Chemistry, Zhejiang University, Hangzhou 310027, People's Republic of China.
Xi
Fengna
F
Gao
Xia
X
Chen
Zhichun
Z
Lin
Xianfu
X
D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis* / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry; D037121:Plant Lectins / Q000378:metabolism*; D012680:Sensitivity and Specificity
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D037121:Plant Lectins; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase