title: Genome response to tissue plasminogen activator in experimental ischemic stroke.
abstract: BACKGROUND
  Tissue plasminogen activator (tPA) is known to have functions beyond fibrinolysis in acute ischemic stroke, such as blood brain barrier disruption. To further delineate tPA functions in the blood, we examined the gene expression profiles induced by tPA in a rat model of ischemic stroke.
  
  
  RESULTS
  tPA differentially expressed 929 genes in the blood of rats (p <or= 0.05, fold change >or= |1.2|). Genes identified had functions related to modulation of immune cells. tPA gene expression was found to be dependent on the reperfusion status of cerebral vasculature. The majority of genes regulated by tPA were different from genes regulated by ischemic stroke.
  
  
  CONCLUSIONS
  tPA modulates gene expression in the blood of rats involving immune cells in a manner that is dependent on the status of vascular reperfusion. These non-fibrinolytic activities of tPA in the blood serve to better understand tPA-related complications.
authors.affiliation: Department of Neurology and M,I,N,D, Institute, University of California at Davis, Sacramento, California 95817, USA. gcjickling@ucdavis.edu
authors.forename: Glen C
authors.initials: GC
authors.lastname: Jickling
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhan
authors.forename: Bradley P
authors.initials: BP
authors.lastname: Ander
authors.forename: Renée J
authors.initials: RJ
authors.lastname: Turner
authors.forename: Boryana
authors.initials: B
authors.lastname: Stamova
authors.forename: Huichun
authors.initials: H
authors.lastname: Xu
authors.forename: Yingfang
authors.initials: Y
authors.lastname: Tian
authors.forename: Dazhi
authors.initials: D
authors.lastname: Liu
authors.forename: Ryan R
authors.initials: RR
authors.lastname: Davis
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lapchak
authors.forename: Frank R
authors.initials: FR
authors.lastname: Sharp
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D002545:Brain Ischemia / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics*; D020869:Gene Expression Profiling; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D020521:Stroke / Q000188:drug therapy* / Q000235:genetics*; D010959:Tissue Plasminogen Activator / Q000627:therapeutic use*
chemical_list: D010959:Tissue Plasminogen Activator
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