title: Selective isolation of Yersinia pestis from plague-infected fleas.
abstract: We evaluated Yersinia CIN agar for the isolation of Yersinia pestis from infected fleas. CIN media is effective for the differentiation of Y. pestis from flea commensal flora and is sufficiently inhibitory to other bacteria that typically outcompete Y. pestis after 48 h of growth using less selective media.
authors.affiliation: Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, USA.
authors.forename: Derek S
authors.initials: DS
authors.lastname: Sarovich
authors.forename: Rebecca E
authors.initials: RE
authors.lastname: Colman
authors.forename: Erin P
authors.initials: EP
authors.lastname: Price
authors.forename: Wai Kwan
authors.initials: WK
authors.lastname: Chung
authors.forename: Judy
authors.initials: J
authors.lastname: Lee
authors.forename: James M
authors.initials: JM
authors.lastname: Schupp
authors.forename: Kacy R
authors.initials: KR
authors.lastname: Cobble
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Busch
authors.forename: James
authors.initials: J
authors.lastname: Alexander
authors.forename: Paul
authors.initials: P
authors.lastname: Keim
authors.forename: David M
authors.initials: DM
authors.lastname: Wagner
mesh_terms: D000818:Animals; D003470:Culture Media / Q000378:metabolism; D007303:Insect Vectors / Q000382:microbiology; D010930:Plague / Q000382:microbiology*; D005423:Siphonaptera / Q000382:microbiology*; D015010:Yersinia pestis / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003470:Culture Media
references.citation: J Med Res. 1917 Jul;36(3):519-32
references.pmid: 19972379
references.citation: J Gen Microbiol. 1958 Oct;19(2):305-11
references.pmid: 13587896
references.citation: Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8408-13
references.pmid: 15173603
references.citation: J Clin Microbiol. 1999 Jun;37(6):1980-4
references.pmid: 10325359
references.citation: Am J Trop Med Hyg. 2000 May;62(5):552-60
references.pmid: 11289663
references.citation: Adv Exp Med Biol. 2003;529:467-8
references.pmid: 12756811
references.citation: J Bacteriol. 1963 Jun;85:1443-5
references.pmid: 14047245
references.citation: J Med Entomol. 1989 Jul;26(4):342-8
references.pmid: 2769715
references.citation: Biotechniques. 2004 Nov;37(5):730, 732, 734
references.pmid: 15560126
references.citation: Am J Trop Med Hyg. 1956 Nov;5(6):1058-70
references.pmid: 13381882
references.citation: J Bacteriol. 2000 May;182(10):2928-36
references.pmid: 10781564
references.citation: J Clin Microbiol. 1993 Jun;31(6):1511-4
references.pmid: 8314993
references.citation: Can J Microbiol. 1979 Nov;25(11):1298-1304
references.pmid: 540256
references.citation: J Med Entomol. 2000 May;37(3):422-6
references.pmid: 15535587
references.citation: J Med Entomol. 2003 May;40(3):329-37
references.pmid: 12943112
references.citation: J Med Entomol. 1993 Jul;30(4):772-80
references.pmid: 8360901
references.citation: Am J Trop Med Hyg. 1960 Jan;9:91-5
references.pmid: 14435458
references.citation: Trans R Soc Trop Med Hyg. 1996 Nov-Dec;90(6):651
references.pmid: 9015505
references.citation: Am J Trop Med Hyg. 1998 May;58(5):562-9
references.pmid: 9598442
references.citation: Appl Environ Microbiol. 2003 Oct;69(10):5787-92
references.pmid: 14532026
references.citation: Bull World Health Organ. 1956;14(3):457-509
references.pmid: 13342926