title: Proteomic and functional characterization of a Chlamydomonas reinhardtii mutant lacking the mitochondrial alternative oxidase 1.
abstract: In the present work, we have isolated by RNA interference and characterized at the functional and the proteomic levels a Chlamydomonas reinhardtii strain devoid of the mitochondrial alternative oxidase 1 (AOX1). The AOX1-deficient strain displays a remarkable doubling of the cell volume and biomass without alteration of the generation time or change in total respiratory rate, with a significantly higher ROS production. To identify the molecular adaptation underlying these observations, we have carried out a comparative study of both the mitochondrial and the cellular soluble proteomes. Our results indicate a strong up-regulation of the ROS scavenging systems and important quantitative modifications of proteins involved in the primary metabolism, namely an increase of enzymes involved in anabolic pathways and a concomitant general down-regulation of enzymes of the main catabolic pathways.
authors.affiliation: Laboratory of Bioenergetics and Cellular Physiology, University of Liege, Belgium.
authors.forename: Grégory
authors.initials: G
authors.lastname: Mathy
authors.forename: Pierre
authors.initials: P
authors.lastname: Cardol
authors.forename: Monique
authors.initials: M
authors.lastname: Dinant
authors.forename: Arnaud
authors.initials: A
authors.lastname: Blomme
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Gérin
authors.forename: Marie
authors.initials: M
authors.lastname: Cloes
authors.forename: Bart
authors.initials: B
authors.lastname: Ghysels
authors.forename: Edwin
authors.initials: E
authors.lastname: DePauw
authors.forename: Pierre
authors.initials: P
authors.lastname: Leprince
authors.forename: Claire
authors.initials: C
authors.lastname: Remacle
authors.forename: Claudine
authors.initials: C
authors.lastname: Sluse-Goffart
authors.forename: Fabrice
authors.initials: F
authors.lastname: Franck
authors.forename: René F
authors.initials: RF
authors.lastname: Matagne
authors.forename: Francis E
authors.initials: FE
authors.lastname: Sluse
mesh_terms: D015153:Blotting, Western; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D020868:Gene Silencing / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010788:Photosynthesis; D010940:Plant Proteins; D011506:Proteins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction; D013347:Subcellular Fractions / Q000378:metabolism; D000099188:Alternative Oxidase
chemical_list: D006861:Hydrogen Peroxide; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D011506:Proteins; D020543:Proteome; D000099188:Alternative Oxidase