DNA interaction studies of new nano metal based anticancer agent: validation by spectroscopic methods.
A new nano dimensional heterobimetallic Cu-Sn containing complex as a potential drug candidate was designed, synthesized and characterized by analytical and spectral methods. The electronic absorption and electron paramagnetic resonance parameters of the complex revealed that the Cu(II) ion exhibits a square pyramidal geometry with the two pyrazole nitrogen atoms, the amine nitrogen atom and the carboxylate oxygen of the phenyl glycine chloride ligand located at the equatorial sites and the coordinated chloride ion occupying an apical position. (119)Sn NMR spectral data showed a hexa-coordinated environment around the Sn(IV) metal ion. TEM, AFM and XRD measurements illustrate that the complex could induce the condensation of CT-DNA to a particulate nanostructure. The interaction of the Cu-Sn complex with CT-DNA was investigated by UV-vis absorption and emission spectroscopy, as well as cyclic voltammetric measurements. The results indicated that the complex interacts with DNA through an electrostatic mode of binding with an intrinsic binding constant K(b) = 8.42 x 10(4) M( - 1). The Cu-Sn complex exhibits effective cleavage of pBR322 plasmid DNA by an oxidative cleavage mechanism, monitored at different concentrations both in the absence and in the presence of reducing agents.
Tabassum
Sartaj
S
Department of Chemistry, Aligarh Muslim University, Aligarh-202002, India.
Chandra Sharma
Girish
G
Arjmand
Farukh
F
Azam
Ameer
A
D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D055664:Electrochemical Techniques; D004578:Electron Spin Resonance Spectroscopy; D004587:Electrophoresis, Agar Gel; D005453:Fluorescence; D009682:Magnetic Resonance Spectroscopy; D008670:Metals / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D013055:Spectrophotometry, Infrared; D013057:Spectrum Analysis / Q000379:methods*; D014961:X-Ray Diffraction
D000970:Antineoplastic Agents; D008670:Metals; D004247:DNA; C427203:calf thymus DNA