Extreme heterogeneous composition of the Paramecium caudatum macronuclear genomic DNA between hemoglobin and nucleosome assembly protein-1 genes.
The intergenic region between the hemoglobin (hb) and nucleosome assembly protein-1 (nap-1) genes in the Paramecium caudatum macronuclear genome was previously found to be heterogeneously composed. Cloning of this intergenic region from the macronuclear genomic DNA identified four unique DNA fragments of different sizes. Sequencing of the cloned fragments revealed extreme heterogeneity and characteristics of both internal eliminated sequence (IES) and imprecise internal deletion sequences (IIDSs) in the intergenic region. Missing sequences were an AT-rich and direct repeats existed in their boundaries. Southern blotting of the total genomic DNA and polymerase chain reaction (PCR) of the total genomic DNAs indicated that there exist a dozen DNA fragments of different sizes in this intergenic region. It is likely that the heterogeneity found in the P. caudatum macronuclear genome results from the variable removal of an intergenic region.
Nishiyama
Norihito
N
Department of Biological Science, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan.
Mikami
Kazuyuki
K
Matsuoka
Ariki
A
Ochiai
Takehiko
T
Yamauchi
Kiyoshi
K
D015139:Blotting, Southern; D021901:DNA, Intergenic / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics*; D005786:Gene Expression Regulation; D018503:Genome, Protozoan; D006454:Hemoglobins / Q000235:genetics*; D048648:Macronucleus / Q000235:genetics*; D056490:Nucleosome Assembly Protein 1 / Q000235:genetics*; D048488:Paramecium caudatum / Q000235:genetics*; D016133:Polymerase Chain Reaction
D021901:DNA, Intergenic; D016054:DNA, Protozoan; D006454:Hemoglobins; D056490:Nucleosome Assembly Protein 1