Upregulation of phagocytic clearance of apoptotic cells by autoimmune regulator.
To investigate the effect of autoimmune regulator (AIRE) on phagocytic clearance of apoptotic cells, a recombinant expression vector containing full-length human AIRE cDNA was transfected into 16HBE cells. After incubation with transfected 16HBE cells, engulfment of apoptotic HL-60 cells induced by camptothecin was detected by myeloperoxidase (MPO) staining. The change in the expression of Rac 1 in transfected 16HBE cells was determined by RT-PCR and Western blotting. The results showed that the phagocytosis percentage of the experimental group, the mock transfection group and the negative control group (non-apoptotic cells) was (25.50 + or - 3.67)%, (6.25 + or - 1.58)% and (1.0 + or - 0.67)%, respectively. Moreover, the expressions of Rac 1 mRNA and protein were up-regulated in AIRE-transfected 16HBE cells, suggesting that AIRE may function as a regulator in the phagocytic clearance of apoptotic cells by promoting the expression of Rac 1.
Shi
Liang
L
Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. liangshi_7@126.com
Hu
Lihua
L
Li
Yirong
Y
D017209:Apoptosis; D015551:Autoimmunity / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005822:Genetic Vectors; D006098:Granulocytes / Q000166:cytology; D018922:HL-60 Cells; D006801:Humans; D010586:Phagocytes / Q000502:physiology; D010587:Phagocytosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*; D000096927:AIRE Protein
D018076:DNA, Complementary; D012333:RNA, Messenger; D014157:Transcription Factors; D020830:rac1 GTP-Binding Protein; D000096927:AIRE Protein; C107953:RAC1 protein, human
J Clin Endocrinol Metab. 1998 Apr;83(4):1049-55
9543115
Nat Genet. 1997 Dec;17(4):393-8
9398839
Dev Cell. 2001 Oct;1(4):491-502
11703940
J Exp Med. 2000 Feb 7;191(3):435-44
10662789
Nat Cell Biol. 2000 Mar;2(3):131-6
10707082
Science. 2002 Nov 15;298(5597):1395-401
12376594
Biochem Biophys Res Commun. 1999 Apr 21;257(3):821-5
10208866
Cell. 2001 Oct 5;107(1):27-41
11595183
Arthritis Rheum. 1998 Jul;41(7):1241-50
9663482
Nat Immunol. 2001 Nov;2(11):1010-7
11590405
Semin Cell Dev Biol. 2005 Apr;16(2):295-306
15797839
J Biol Chem. 2002 Apr 5;277(14):11772-9
11729193
Nature. 1998 Apr 2;392(6675):505-9
9548256
Cell. 1998 Jun 12;93(6):951-60
9635425
Immunol Lett. 2002 Mar 1;80(3):195-8
11803052
Cell Death Differ. 2001 Jun;8(6):564-8
11536006
Mol Cell. 2004 May 7;14(3):277-87
15125832
Cell Death Differ. 1999 Jan;6(1):6-12
10200542
Proc Natl Acad Sci U S A. 2005 May 17;102(20):7233-8
15883360
Biochim Biophys Acta. 2000 Feb 28;1495(3):237-49
10699463
Nat Genet. 1997 Dec;17(4):399-403
9398840
Immunity. 2005 Aug;23(2):227-39
16111640
J Allergy Clin Immunol. 2009 Dec;124(6):1161-78
20004777
J Biol Chem. 2004 Aug 6;279(32):33984-91
15150263
Clin Immunol. 2006 Dec;121(3):265-73
16982213