Quercetin and kaempferol suppress immunoglobulin E-mediated allergic inflammation in RBL-2H3 and Caco-2 cells.
OBJECTIVE
We investigated the inhibitory effects of quercetin and kaempferol treatment on the suppression of immunoglobulin E (IgE)-mediated allergic responses in relation to intestinal epithelium barrier function in RBL-2H3 and Caco-2 cells.


METHODS
RBL-2H3 cells as a model of intestinal mucosa mast cells were treated with flavonols followed by IgE-anti-dinitrophenyl sensitization. The extent of degranulation and the release of pro-inflammatory cytokines were measured. Caco-2 cells were stimulated with interleukin (IL)-4 or IgE-allergen with or without flavonol pretreatment and changes in the expression of CD23 mRNA and mitogen-activated protein kinase (MAPK), and chemokine release were determined.


RESULTS
Flavonols inhibited the secretion of allergic mediators in RBL-2H3 cells and suppressed the CD23 mRNA expression and p38 MAPK activation in IL-4 stimulated Caco-2 cells. Flavonols also suppressed IgE-OVA induced extra signal-regulated protein kinase (ERK) activation and chemokine release.


CONCLUSIONS
Quercetin and kaempferol effectively suppressed the development of IgE-mediated allergic inflammation of intestinal cell models.
Lee
Eun-Ju
EJ
Department of Food and Nutrition, Sookmyung Women's University, Seoul 140-742, Korea.
Ji
Geun-Eok
GE
Sung
Mi-Kyung
MK
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D018938:Caco-2 Cells / Q000187:drug effects / Q000276:immunology; D054418:Chemokine CCL20 / Q000276:immunology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000276:immunology; D005512:Food Hypersensitivity / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D007249:Inflammation / Q000276:immunology*; D016209:Interleukin-8 / Q000276:immunology; D007413:Intestinal Mucosa / Q000166:cytology; D044949:Kaempferols / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D015394:Molecular Structure; D011794:Quercetin / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D017455:Receptors, IgE / Q000235:genetics / Q000276:immunology; D048051:p38 Mitogen-Activated Protein Kinases / Q000276:immunology
D000975:Antioxidants; D054418:Chemokine CCL20; D016209:Interleukin-8; D044949:Kaempferols; D017455:Receptors, IgE; D007073:Immunoglobulin E; C006552:kaempferol; D011794:Quercetin; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases
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