title: Identification and molecular characterization of begomovirus and associated satellite DNA molecules infecting Cyamopsis tetragonoloba.
abstract: Monopartite begomoviruses comprise DNA-A as the main genome and associated satellite DNAs. Viral DNA extracted from guar (Cyamopsis tetragonoloba) showing leaf curl symptoms exhibited positive amplification of coat protein (CP) gene of DNA-A component, suggesting the presence of begomovirus. Full length DNA-A was amplified by primer pair re-designed from CP gene nucleotide sequence. The associated alphasatellite and betasatellite DNA molecules were amplified and sequenced, confirming the presence of monopartite begomovirus. Sequence comparisons showed 89% identity with other begomoviruses. The Neighbor-Joining tree based on full length DNA-A nucleotide sequence showed that the guar infecting begomovirus clustered separately from other known begomoviruses. The betasatellite shared a high (96%) nucleotide identity to Cotton leaf curl Multan betasatellites. The alphasatellite showed 91% nucleotide identity to alphasatellite associated with begomovirus infecting Okra. Recombination analyses showed three recombinant fragments in DNA-A, two in betasatellite, and four in alphasatellite. The results suggest that the begomovirus identified in this study was a new recombinant virus. Its name was proposed as Cyamopsis tetragonoloba leaf curl virus (CyTLCuV).
authors.affiliation: Molecular Virology Laboratory, National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, India. jitsingh27281@gmail.com
authors.forename: J
authors.initials: J
authors.lastname: Kumar
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: J K
authors.initials: JK
authors.lastname: Roy
authors.forename: R
authors.initials: R
authors.lastname: Tuli
authors.forename: J A
authors.initials: JA
authors.lastname: Khan
mesh_terms: D053839:Begomovirus / Q000235:genetics* / Q000302:isolation & purification; D031293:Cyamopsis / Q000821:virology*; D004274:DNA, Recombinant; D004276:DNA, Satellite / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA
chemical_list: D004274:DNA, Recombinant; D004276:DNA, Satellite
references.citation: Arch Virol. 2008;153(4):763-81
references.pmid: 18247103
references.citation: Arch Virol. 1994;139(1-2):49-77
references.pmid: 7826215
references.citation: Acta Virol. 2001 Feb;45(1):45-50
references.pmid: 11394577
references.citation: J Gen Virol. 2001 Mar;82(Pt 3):655-665
references.pmid: 11172108
references.citation: Virology. 2001 Jul 5;285(2):234-43
references.pmid: 11437658
references.citation: Virology. 2003 Jul 20;312(1):106-21
references.pmid: 12890625
references.citation: Virology. 2003 Jan 20;305(2):310-7
references.pmid: 12573576
references.citation: Arch Virol. 1994;135(1-2):165-70
references.pmid: 8198442
references.citation: Virus Genes. 2002 Jun;24(3):249-56
references.pmid: 12086146
references.citation: Virology. 2001 Mar 30;282(1):38-47
references.pmid: 11259188
references.citation: J Gen Virol. 1993 Jan;74 ( Pt 1):147-51
references.pmid: 8423446
references.citation: Virology. 1999 Nov 10;264(1):142-52
references.pmid: 10544139
references.citation: Virology. 2002 Nov 25;303(2):317-26
references.pmid: 12490393
references.citation: Phytopathology. 2004 Oct;94(10):1068-74
references.pmid: 18943795
references.citation: J Gen Virol. 1995 Dec;76 ( Pt 12):2915-22
references.pmid: 8847495
references.citation: Virology. 1999 Dec 20;265(2):218-25
references.pmid: 10600594
references.citation: Virology. 1991 Nov;185(1):151-61
references.pmid: 1926771
references.citation: J Gen Virol. 2007 Oct;88(Pt 10):2881-2889
references.pmid: 17872543
references.citation: Arch Virol. 2008;153(4):783-821
references.pmid: 18256781
references.citation: J Gen Virol. 1998 Apr;79 ( Pt 4):649-57
references.pmid: 9568957
references.citation: Nucleic Acids Res. 1991 Dec 25;19(24):6763-9
references.pmid: 1840676
references.citation: J Gen Virol. 2003 Jan;84(Pt 1):237-247
references.pmid: 12533720
references.citation: Virology. 1999 Jun 20;259(1):190-9
references.pmid: 10364503
references.citation: Nature. 2003 Apr 24;422(6934):831
references.pmid: 12712190