Comparison of red blood cell membrane microstructure after different physicochemical influences: atomic force microscope research.
OBJECTIVE
After the influence of different actions on the blood, the erythrocytes may change their macrostructure. At the same time, the microstructure of cell membrane will be changed as well. This study provides the results of comparison of red blood cell membrane microstructure after they have been affected by different factors.


METHODS
Images and spatial profiles of the cell surface were obtained by atomic force microscope. It was proposed to use spatial Fourier transform to decompose the initial complex profile into series of simple ones. This made it possible to compare surface parameters after exposure of red blood cells to different external actions.


RESULTS
Quantitative differences between membrane profile harmonic composition parameters (amplitude and spatial period) after physical impact (impulse electrical field, osmotic swelling) and after chemical impact (the fixing fluid glutaraldehyde and the drug Esmeron) were experimentally confirmed.


CONCLUSIONS
Such experimental and theoretical approach may lay down the foundations of mechanisms of different factors' effect on red blood cells both in research and in clinics.
Moroz
Victor V
VV
V.A. Negovsky Research Institute of General Reanimatology, Russian Academy of Medical Sciences, Moscow, Russia.
Chernysh
Alexander M
AM
Kozlova
Elena K
EK
Borshegovskaya
Polina Yu
PY
Bliznjuk
Ulyana A
UA
Rysaeva
Regina M
RM
Gudkova
Olga Ye
OY
D000328:Adult; D000732:Androstanols / Q000494:pharmacology; D004560:Electricity; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000648:ultrastructure*; D005404:Fixatives / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D003473:Neuromuscular Nondepolarizing Agents / Q000494:pharmacology; D009995:Osmosis; D000077123:Rocuronium; D013499:Surface Properties
D000732:Androstanols; D005404:Fixatives; D003473:Neuromuscular Nondepolarizing Agents; D005976:Glutaral; D000077123:Rocuronium