title: Polarized fluorescent nanospheres.
abstract: Fluorescent beads (nanoparticles, nanospheres) are commonly used in fluorescence spectroscopy and microscopy. Due to the random distribution of dye and high dye to nanoparticle ratio, the fluorescence polarization observed from the beads is low. Therefore beads are not used for polarization study. We demonstrate that photoselective bleaching creates beads with highly polarized fluorescence. First, the beads were immobilized in a PVA polymer. Second, the beads-doped PVA film was exposed to the illumination within the dye absorption band. A progressive decrease of absorption was observed. Next, photophysical properties of photobleached and not bleached films dissolved in water were compared.
authors.affiliation: Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Center for Commercialization of Fluorescence Technologies, 3500 Camp Bowie Boulevard, TX, Fort Worth, 76107, USA.
authors.forename: Rafal
authors.initials: R
authors.lastname: Luchowski
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Gryczynski
authors.forename: Zeno
authors.initials: Z
authors.lastname: Földes-Papp
authors.forename: Aaron
authors.initials: A
authors.lastname: Chang
authors.forename: Julian
authors.initials: J
authors.lastname: Borejdo
authors.forename: Pabak
authors.initials: P
authors.lastname: Sarkar
authors.forename: Ignacy
authors.initials: I
authors.lastname: Gryczynski
mesh_terms: D000042:Absorption; D005453:Fluorescence; D005454:Fluorescence Polarization; D054118:Nanospheres / Q000737:chemistry*; D038761:Photobleaching; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D011142:Polyvinyl Alcohol