title: Host-encoded reporters for the detection and purification of multiple enveloped viruses.
abstract: The identification of host cell factors for virus replication holds great promise for the development of new antiviral therapies. Recently, high-throughput screening methods have emerged as powerful tools to identify candidate host factors for therapeutic intervention. The development of assay systems suitable for large-scale automated screening is of particular importance for novel viruses with high pathogenic potential for which limited biological information can be developed in a short period of time. This report presents a general enzymatic reporter system for the detection and characterization of multiple enveloped viruses that does not rely on engineering of the virus. Instead, reporter enzymes are incorporated into virus particles by targeting to lipid microdomains in producer cells. The approach allows a variety of human pathogenic enveloped viruses to be detected by sensitive, inexpensive and automatable enzymatic assays. Tagged viruses can be purified quickly and efficiently by a magnetic bead-based capture method. The method allows general detection of enveloped viruses without prior reference to their sequence.
authors.affiliation: Center for Computational and Integrative Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA.
authors.forename: Robin
authors.initials: R
authors.lastname: Ketteler
authors.forename: Vesselin
authors.initials: V
authors.lastname: Tomov
authors.forename: Alina
authors.initials: A
authors.lastname: Neunkirchner
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xie
authors.forename: Winfried F
authors.initials: WF
authors.lastname: Pickl
authors.forename: Brian
authors.initials: B
authors.lastname: Seed
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D057166:High-Throughput Screening Assays / Q000379:methods*; D054884:Host-Pathogen Interactions; D006801:Humans; D013194:Staining and Labeling / Q000379:methods; D014771:Virion / Q000302:isolation & purification; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology; D014780:Viruses / Q000302:isolation & purification*
chemical_list: D000469:Alkaline Phosphatase
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