Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by cetyltrimethylammonium bromide-permeabilized cells of Alcaligenes faecalis ECU0401.
The nitrilase from Alcaligenes faecalis ECU0401 belongs to the category of arylacetonitrilase, which could hydrolyze 2-chloromandelonitrile, 3,4-dimethoxyphenylacetonitrile, mandelonitrile, and phenylacetonitrile into the corresponding arylacetic acids. To overcome the permeability barrier and prepare whole cell biocatalysts with high activities, permeabilization of Alcaligenes faecalis ECU0401 in relation to nitrilase activity was optimized by using cetyltrimethylammonium bromide (CTAB) as permeabilizing agent. The nitrilase activity from Alcaligenes faecalis ECU0401 increased 4.5-fold when the cells were permeabilized with 0.3% (w/v) CTAB for 20 min at 25 degrees C and pH 6.5. Consequently, almost all the mandelonitrile was consumed and converted to (R)-(-)-mandelic acid with greater than 99.9% enantiomeric excess (e.e.) by the CTAB-permeabilized cells. The permeability barrier has been significantly reduced in the hydrolysis of mandelonitrile by using CTAB-permeabilized cells and a dynamic resolution was successfully achieved, giving a 100% theoretical yield of (R)-(-)-mandelic acid. Efficient biocatalyst recycling was achieved as a result of cell immobilization in calcium alginate, with a product-to-biocatalyst ratio of 3.82 g (R)-(-)-mandelic acid g(-1) dry cell weight (dcw) cell after 20 cycles of repeated use.
He
Yu-Cai
YC
Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China. yucaihe2007@yahoo.com.cn
Zhang
Zhi-Jun
ZJ
Xu
Jian-He
JH
Liu
You-Yan
YY
D000097:Acetonitriles / Q000737:chemistry / Q000378:metabolism*; D042481:Alcaligenes faecalis / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D018914:Cells, Immobilized / Q000737:chemistry / Q000378:metabolism; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D008333:Mandelic Acids / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism
D000097:Acetonitriles; D002593:Cetrimonium Compounds; D008333:Mandelic Acids; C045516:mandelonitrile; C037938:mandelic acid; D000077286:Cetrimonium
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