title: A domain level interaction network of amyloid precursor protein and Abeta of Alzheimer's disease.
abstract: The primary constituent of the amyloid plaque, beta-amyloid (Abeta), is thought to be the causal "toxic moiety" of Alzheimer's disease. However, despite much work focused on both Abeta and its parent protein, amyloid precursor protein (APP), the functional roles of APP and its cleavage products remain to be fully elucidated. Protein-protein interaction networks can provide insight into protein function, however, high-throughput data often report false positives and are in frequent disagreement with low-throughput experiments. Moreover, the complexity of the CNS is likely to be under represented in such databases. Therefore, we curated the published work characterizing both APP and Abeta to create a protein interaction network of APP and its proteolytic cleavage products, with annotation, where possible, to the level of APP binding domain and isoform. This is the first time that an interactome has been refined to domain level, essential for the interpretation of APP due to the presence of multiple isoforms and processed fragments. Gene ontology and network analysis were used to identify potentially novel functional relationships among interacting proteins.
authors.affiliation: Neuroproteomics and Neurogenomics Platform, National Neurosciences Facility, The University of Melbourne, Parkville, VIC, Australia. vperreau@unimelb.edu.au
authors.forename: Victoria M
authors.initials: VM
authors.lastname: Perreau
authors.forename: Sandra
authors.initials: S
authors.lastname: Orchard
authors.forename: Paul A
authors.initials: PA
authors.lastname: Adlard
authors.forename: Shayne A
authors.initials: SA
authors.lastname: Bellingham
authors.forename: Roberto
authors.initials: R
authors.lastname: Cappai
authors.forename: Giuseppe D
authors.initials: GD
authors.lastname: Ciccotosto
authors.forename: Tiffany F
authors.initials: TF
authors.lastname: Cowie
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Crouch
authors.forename: James A
authors.initials: JA
authors.lastname: Duce
authors.forename: Genevieve
authors.initials: G
authors.lastname: Evin
authors.forename: Noel G
authors.initials: NG
authors.lastname: Faux
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Hill
authors.forename: Ya Hui
authors.initials: YH
authors.lastname: Hung
authors.forename: Simon A
authors.initials: SA
authors.lastname: James
authors.forename: Qiao-Xin
authors.initials: QX
authors.lastname: Li
authors.forename: Su San
authors.initials: SS
authors.lastname: Mok
authors.forename: Deborah J
authors.initials: DJ
authors.lastname: Tew
authors.forename: Anthony R
authors.initials: AR
authors.lastname: White
authors.forename: Ashley I
authors.initials: AI
authors.lastname: Bush
authors.forename: Henning
authors.initials: H
authors.lastname: Hermjakob
authors.forename: Colin L
authors.initials: CL
authors.lastname: Masters
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D049490:Systems Biology
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor