title: Histological changes in the uterus of the hens after embryonic exposure to bisphenol A and diethylstilbestrol.
abstract: Many employed chemicals in industries have estrogenic hormone effects on organisms, and these are called as environmental estrogens. Environmental estrogens have adverse effects on development and function of reproductive organs of the birds. Bisphenol A (BPA) is one of the best known environmental estrogens widely found in plastic products. In this study, we injected BPA and the synthetic estrogen diethylstilbestrol (DES) in ovo and then examined and compared the effects of those on the uteri (shell gland) of the adult hens by histological methods. Five groups have been designed in the current study. Only vehicle substance was given in ovo to the control group and BPA (67 or 134 μg/g egg) and DES (0.02 or 0.2 μg/g egg) were administered in the experimental groups. Tissue specimens were taken from uteri of hens at 21 weeks of age, prior to the laying period. Estrogen receptor alpha (ERα) was immunohistochemically stained. It was observed that the hatching proportion in BPA (67 μg and 134 μg/g) was lesser than the other groups (P<0.01). Uterine tubular glandular density and thickness of tunica mucosa were found to have reduced (P<0.01) in BPA (134 μg/g) and DES (0.2 μg/g) groups, in comparison with those of the control and the other experimental groups. Uterine gland epithelium revealed positive immunoreaction for ERα. These findings suggested that administration of BPA and DES at high doses affected embryonic development in a negative way, and this adverse effect was seen less in adult period.
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey. fyigit@istanbul.edu.tr
authors.forename: Funda
authors.initials: F
authors.lastname: Yigit
authors.forename: Suzan
authors.initials: S
authors.lastname: Daglioglu
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D002645:Chickens; D004054:Diethylstilbestrol / Q000633:toxicity*; D004848:Epithelium / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D045166:Estradiol Congeners / Q000633:toxicity*; D047628:Estrogen Receptor alpha / Q000378:metabolism; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D010057:Oviducts / Q000033:anatomy & histology / Q000187:drug effects*; D010063:Ovum / Q000187:drug effects*; D010636:Phenols / Q000633:toxicity*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004054:Diethylstilbestrol; D045166:Estradiol Congeners; D047628:Estrogen Receptor alpha; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
references.citation: Regul Toxicol Pharmacol. 1997 Aug;26(1 Pt 1):60-8
references.pmid: 9339481
references.citation: J Steroid Biochem Mol Biol. 1998 Apr;65(1-6):143-50
references.pmid: 9699867
references.citation: Brain Res. 2000 Nov 3;882(1-2):236-40
references.pmid: 11056207
references.citation: Res Vet Sci. 2004 Feb;76(1):63-8
references.pmid: 14659731
references.citation: Environ Toxicol Chem. 2001 Dec;20(12):2836-40
references.pmid: 11764168
references.citation: J Vet Sci. 2007 Sep;8(3):219-22
references.pmid: 17679766
references.citation: Mol Cell Endocrinol. 1990 Mar 5;69(2-3):235-43
references.pmid: 2328829
references.citation: J Steroid Biochem Mol Biol. 2002 Dec;83(1-5):235-44
references.pmid: 12650721
references.citation: J Reprod Fertil. 1984 Jul;71(2):411-7
references.pmid: 6747950
references.citation: Environ Health Perspect. 2000 Aug;108(8):723-9
references.pmid: 10964792
references.citation: Endocrinology. 1998 Oct;139(10):4252-63
references.pmid: 9751507
references.citation: Reproduction. 2001 Jan;121(1):155-65
references.pmid: 11226039
references.citation: Environ Health Perspect. 2003 Jun;111(8):994-1006
references.pmid: 12826473
references.citation: Neoplasia. 2004 Sep-Oct;6(5):584-94
references.pmid: 15548368
references.citation: Endocr J. 2002 Aug;49(4):465-71
references.pmid: 12402979
references.citation: Life Sci. 2006 Mar 6;78(15):1767-76
references.pmid: 16297413
references.citation: Fertil Steril. 2008 Oct;90(4):911-40
references.pmid: 18929049
references.citation: Domest Anim Endocrinol. 2005 Aug;29(2):436-45
references.pmid: 15950431
references.citation: Chemosphere. 1998 Apr;36(10):2149-73
references.pmid: 9566294
references.citation: Sci Total Environ. 1999 Aug 15;233(1-3):57-66
references.pmid: 10492898
references.citation: Reproduction. 2008 Aug;136(2):175-86
references.pmid: 18483074
references.citation: Toxicol Appl Pharmacol. 1997 Mar;143(1):205-12
references.pmid: 9073609
references.citation: Biochem J. 1985 Jun 15;228(3):689-95
references.pmid: 4026804
references.citation: Regul Toxicol Pharmacol. 1999 Oct;30(2 Pt 1):156-66
references.pmid: 10536110
references.citation: J Cell Biol. 1969 Jan;40(1):8-27
references.pmid: 5782453
references.citation: Food Addit Contam. 2001 Jan;18(1):69-75
references.pmid: 11212549
references.citation: Poult Sci. 2003 Oct;82(10):1624-9
references.pmid: 14601742
references.citation: Crit Rev Biochem Mol Biol. 2002;37(1):1-28
references.pmid: 11905545
references.citation: Environ Health Perspect. 1995 Oct;103 Suppl 7:165-71
references.pmid: 8593865
references.citation: J Steroid Biochem Mol Biol. 1992 Mar;42(1):23-8
references.pmid: 1558818
references.citation: Arch Toxicol. 2006 Dec;80(12):857-67
references.pmid: 16710698
references.citation: Arch Toxicol. 2001 Dec;75(10):597-603
references.pmid: 11808920
references.citation: Teratology. 1985 Feb;31(1):101-9
references.pmid: 3983852
references.citation: Environ Health Perspect. 1993 Oct;101(5):378-84
references.pmid: 8080506
references.citation: Chemosphere. 1999 May;38(11):2569-76
references.pmid: 10204238