Mast cells and angiogenesis in gastric carcinoma.
Previous studies have shown that increased vascularity is associated with haematogenous metastasis and poor prognosis in gastric cancer. The role of mast cells in gastric cancer angiogenesis has not been clarified completely. In this study, we correlated microvascular density and tryptase- and chymase-positive mast cells with histopathological type in gastric cancer. Specimens of primary gastric adenocarcinomas obtained from 30 patients who had undergone curative gastrectomy were investigated immunohistochemically by using anti-CD31 antibody to stain endothelial cells and anti-tryptase and anti-chymase antibodies to stain mast cells. The results showed that stage IV gastric carcinoma has a higher degree of vascularization than other stages and that both tryptase- and chymase-positive mast cells increase in parallel with malignancy grade even if the density of chymase-positive mast cells was significantly lower than the density of tryptase-positive mast cells and is highly correlated with the extent of angiogenesis. This study has demonstrated that mast cell density correlates with angiogenesis and progression of patients with gastric carcinoma. Understanding the mechanisms of gastric cancer angiogenesis provides a basis for a rational approach to the development of an antiangiogenic therapy in patients with this malignancy.
Ribatti
Domenico
D
Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy. ribatti@anatomia.uniba.it
Guidolin
Diego
D
Marzullo
Andrea
A
Nico
Beatrice
B
Annese
Tiziana
T
Benagiano
Vincenzo
V
Crivellato
Enrico
E
D000230:Adenocarcinoma / Q000098:blood supply* / Q000473:pathology* / Q000601:surgery; D000328:Adult; D000368:Aged; D053818:Chymases / Q000032:analysis; D005260:Female; D005743:Gastrectomy; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000201:enzymology / Q000473:pathology*; D055806:Microvessels / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000276:immunology / Q000473:pathology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D013274:Stomach Neoplasms / Q000098:blood supply* / Q000473:pathology* / Q000601:surgery; D053802:Tryptases / Q000032:analysis
D019408:Platelet Endothelial Cell Adhesion Molecule-1; D053818:Chymases; D053802:Tryptases
Histopathology. 2003 Mar;42(3):246-50
12605644
J Clin Invest. 1997 Jun 1;99(11):2691-700
9169499
J Biol Chem. 1995 Mar 3;270(9):4689-96
7876240
Am J Obstet Gynecol. 2005 Dec;193(6):1961-5
16325597
J Surg Oncol. 1997 Aug;65(4):232-6
9274786
Eur J Cancer. 2003 Mar;39(5):666-74
12628847
Clin Cancer Res. 1998 Feb;4(2):429-34
9516932
Virchows Arch. 2000 Mar;436(3):243-8
10782883
Cancer. 1999 Oct 15;86(8):1455-62
10526273
Int Rev Cell Mol Biol. 2009;275:89-131
19491054
Neurobiol Aging. 2008 Jun;29(6):926-36
17258840
Mol Cancer Ther. 2004 Sep;3(9):1041-8
15367698
Leukemia. 2007 Jan;21(1):44-52
16990761
Br J Cancer. 1999 Feb;79(3-4):451-5
10027312
J Biol Chem. 1994 Apr 1;269(13):9416-9
8144524
Cancer. 1996 Mar 1;77(5):858-63
8608475
J Clin Invest. 1989 Nov;84(5):1657-62
2553780
Pediatrics. 1982 Jul;70(1):48-51
7088632
Indian J Pathol Microbiol. 2009 Jan-Mar;52(1):20-4
19136773
Ann Thorac Surg. 2000 Jun;69(6):1686-90
10892907
Leukemia. 2002 Sep;16(9):1680-4
12200681
Hum Pathol. 2000 Aug;31(8):955-60
10987256
Am J Pathol. 1998 Jan;152(1):93-100
9422527
J Histochem Cytochem. 2001 Aug;49(8):1061-2
11457936
Br J Cancer. 1998 Jun;77(11):1900-6
9667666
Int J Cancer. 2000 Jan 15;85(2):171-5
10629073
Int J Mol Med. 2006 Jun;17(6):981-7
16685405
Br J Cancer. 1997;75(4):566-71
9052412
Int J Cancer. 1998 Sep 11;77(6):933-6
9714067
J Clin Oncol. 1995 Feb;13(2):477-81
7531224
Eur J Haematol. 2003 Aug;71(2):137-9
12890156
Leukemia. 2003 Jul;17(7):1428-30
12835741
Surg Gynecol Obstet. 1966 Jul;123(1):3-9
5947123
J Clin Pathol. 2001 Dec;54(12):940-4
11729214
Clin Exp Allergy. 2004 Nov;34(11):1660-4
15544587
Dis Colon Rectum. 1995 Mar;38(3):290-3
7882795
World J Gastroenterol. 2002 Dec;8(6):994-8
12439912
Oncogene. 2006 Jul 20;25(31):4257-66
16518413
Methods Mol Biol. 2006;315:53-62
16110148
Cancer Res. 1996 Jun 1;56(11):2671-6
8653715
Clin Cancer Res. 1996 Oct;2(10):1679-84
9816116
Br J Cancer. 2000 Jan;82(1):167-70
10638985
Br J Cancer. 1999 Oct;81(4):647-53
10574250
Nat Clin Pract Oncol. 2005 Nov;2(11):562-77
16270097
J Clin Oncol. 1997 Feb;15(2):826-32
9053510
Eur J Clin Invest. 2003 May;33(5):420-5
12760367
Cancer Res. 2001 Mar 1;61(5):2145-53
11280779
J Clin Oncol. 2006 Nov 20;24(33):5201-6
17114652
Surgery. 2000 Sep;128(3):408-16
10965312
Anticancer Res. 2005 Jan-Feb;25(1A):17-22
15816514
J Cell Mol Med. 2009 Sep;13(9A):2822-33
19538473