title: Chemical composition and antimicrobial activity of the essential oils from New Caledonian Citrus macroptera and Citrus hystrix.
abstract: The essential oils from the leaves of Citrus macroptera and C. hystrix, collected in New Caledonia, have been analyzed by gas chromatography/mass spectrometry (GC/MS) and evaluated for their antimicrobial activity. A total of 35 and 38 constituents were identified, representing 99.1 and 89.0% of the essential oils, respectively. Both essential oils were rich in monoterpenes (96.1 and 87.0%, resp.), with beta-pinene as major component (33.3 and 10.9%, resp.), and poor in limonene (2.4 and 4.7%, resp.). Other main components of C. macroptera oil were alpha-pinene (25.3%), p-cimene (17.6%), (E)-beta-ocimene (6.7%), and sabinene (4.8%). The essential oil of C. hystrix was characterized by high contents of terpinen-4-ol (13.0%), alpha-terpineol (7.6%), 1,8-cineole (6.4%), and citronellol (6.0%). The antimicrobial activity was evaluated against five bacteria and five fungi strains. Both oils were inactive against bacteria. However, the C. macroptera leaf oil exhibited a pronounced activity against Trichophyton mentagrophytes var. interdigitale, with a minimal-inhibitory concentration (MIC) of 12.5 microg/ml.
authors.affiliation: Laboratoire Substances Naturelles Terrestres & Savoirs Traditionnels ('SNT&ST'), DRV, IRD US084, BPA5, 98848 Nouméa, Nouvelle-Calédonie.
authors.forename: Jean
authors.initials: J
authors.lastname: Waikedre
authors.forename: Annabelle
authors.initials: A
authors.lastname: Dugay
authors.forename: Isabel
authors.initials: I
authors.lastname: Barrachina
authors.forename: Christine
authors.initials: C
authors.lastname: Herrenknecht
authors.forename: Pierre
authors.initials: P
authors.lastname: Cabalion
authors.forename: Alain
authors.initials: A
authors.lastname: Fournet
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D002957:Citrus / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D009822:Oils, Volatile