title: Indoleamine 2,3-dioxygenase 1 is a lung-specific innate immune defense mechanism that inhibits growth of Francisella tularensis tryptophan auxotrophs.
abstract: Upon microbial challenge, organs at various anatomic sites of the body employ different innate immune mechanisms to defend against potential infections. Accordingly, microbial pathogens evolved to subvert these organ-specific host immune mechanisms to survive and grow in infected organs. Francisella tularensis is a bacterium capable of infecting multiple organs and thus encounters a myriad of organ-specific defense mechanisms. This suggests that F. tularensis may possess specific factors that aid in evasion of these innate immune defenses. We carried out a microarray-based, negative-selection screen in an intranasal model of Francisella novicida infection to identify Francisella genes that contribute to bacterial growth specifically in the lungs of mice. Genes in the bacterial tryptophan biosynthetic pathway were identified as being important for F. novicida growth specifically in the lungs. In addition, a host tryptophan-catabolizing enzyme, indoleamine 2,3-dioxygenase 1 (IDO1), is induced specifically in the lungs of mice infected with F. novicida or Streptococcus pneumoniae. Furthermore, the attenuation of F. novicida tryptophan mutant bacteria was rescued in the lungs of IDO1(-/-) mice. IDO1 is a lung-specific innate immune mechanism that controls pulmonary Francisella infections.
authors.affiliation: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Kaitian
authors.initials: K
authors.lastname: Peng
authors.forename: Denise M
authors.initials: DM
authors.lastname: Monack
mesh_terms: D000818:Animals; D053898:Biosynthetic Pathways / Q000235:genetics; D002460:Cell Line; D015169:Colony Count, Microbial; D005260:Female; D005604:Francisella tularensis / Q000276:immunology* / Q000472:pathogenicity; D020869:Gene Expression Profiling; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000276:immunology*; D008168:Lung / Q000276:immunology* / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D013154:Spleen / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000276:immunology / Q000472:pathogenicity; D014364:Tryptophan / Q000096:biosynthesis; D037521:Virulence Factors / Q000096:biosynthesis
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D037521:Virulence Factors; D014364:Tryptophan
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