Astaxanthin improves the proliferative capacity as well as the osteogenic and adipogenic differentiation potential in neural stem cells.
In the present study, the effect of astaxanthin on improvement of the proliferative capacity as well as the osteogenic and adipogenic differentiation potential in neural stem cells (NSCs) was evaluated. Treatment of astaxanthin-induced actives cell growth in a dose-dependent and time-dependent manner. Results from a clonogenic assay clearly indicated that astaxanthin can actively stimulate proliferation of NSCs. Astaxanthin-induced improvement in the proliferative capacity of NSCs resulted in overexpression of several proliferation-related proteins. Astaxanthin-induced activation of PI3K and its downstream mediators, p-MEK, p-ERK, and p-Stat3 in NSCs resulted in subsequent induction of expression of proliferation-related transcription factors (Rex1, CDK1, and CDK2) and stemness genes (OCT4, SOX2, Nanog, and KLF4). Astaxanthin also improved the osteogenic and adipogenic differentiation potential of NSCs. Astaxanthin-treated NSCs showed prominent calcium deposits and fat formation. These results were consistent with overexpression of osteogenesis-related genes (osteonectin, RXR, and osteopontin) and adipogenesis-related genes (AP and PPAR-gamma) after astaxanthin treatment. These findings clearly demonstrated that astaxanthin acts synergistically on the regulatory circuitry that controls proliferation and differentiation of NSCs.
Kim
Jeong Hwan
JH
Department of Biomaterial Control, Dong-Eui University, Busan, South Korea.
Nam
Soo-Wan
SW
Kim
Byung Woo
BW
Kim
Wun-Jae
WJ
Choi
Yung Hyun
YH
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D000090062:Kruppel-Like Factor 4; D051379:Mice; D009420:Nervous System / Q000166:cytology / Q000187:drug effects*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D024341:Xanthophylls / Q000494:pharmacology
C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D024341:Xanthophylls; C005948:astaxanthine