title: Expression profiling of cumulus cells reveals functional changes during ovulation and central roles of prostaglandin EP2 receptor in cAMP signaling.
abstract: To understand the role of prostaglandin (PG) receptor EP2 (Ptger2) signaling in ovulation and fertilization, we investigated time-dependent expression profiles in wild-type (WT) and Ptger2(-/-) cumuli before and after ovulation by using microarrays. We prepared cumulus cells from mice just before and 3, 9 and 14 h after human chorionic gonadotropin injection. Key genes including cAMP-related and epidermal growth factor (EGF) genes, as well as extracellular matrix- (ECM-) related and chemokine genes were up-regulated in WT cumuli at 3 h and 14 h, respectively. Ptger2 deficiency differently affected the expression of many of the key genes at 3 h and 14 h. These results indicate that the gene expression profile of cumulus cells greatly differs before and after ovulation, and in each situation, PGE(2)-EP2 signaling plays a critical role in cAMP-regulated gene expression in the cumulus cells under physiological conditions.
authors.affiliation: Department of Physiological Chemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Shigero
authors.initials: S
authors.lastname: Tamba
authors.forename: Rieko
authors.initials: R
authors.lastname: Yodoi
authors.forename: Kazushi
authors.initials: K
authors.lastname: Morimoto
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Inazumi
authors.forename: Mamiko
authors.initials: M
authors.lastname: Sukeno
authors.forename: Eri
authors.initials: E
authors.lastname: Segi-Nishida
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okuno
authors.forename: Gozoh
authors.initials: G
authors.lastname: Tsujimoto
authors.forename: Shuh
authors.initials: S
authors.lastname: Narumiya
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Sugimoto
mesh_terms: D000818:Animals; D054885:Cumulus Cells / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010060:Ovulation / Q000235:genetics* / Q000378:metabolism; D018078:Receptors, Prostaglandin E / Q000235:genetics / Q000378:metabolism*; D058307:Receptors, Prostaglandin E, EP2 Subtype; D015398:Signal Transduction
chemical_list: C545644:PTGER2 protein, human; C545645:Ptger2 protein, mouse; D018078:Receptors, Prostaglandin E; D058307:Receptors, Prostaglandin E, EP2 Subtype; D000242:Cyclic AMP