title: Nucleophosmin/B23 inhibits Eg5-mediated microtubule depolymerization by inactivating its ATPase activity.
abstract: Nucleophosmin/B23, an abundant nucleolar protein, plays multiple roles in cell growth and proliferation, and yet, little has been studied about its function in regulating dynamics of microtubules. Here, we report that B23 directly interacts with Eg5, a member of the kinesin family, in the cytosol. The DNA/RNA binding domain of B23 and the motor domain of Eg5 were found to be involved in their interaction. Both in vivo and in vitro evidences showed that B23 acts as an upstream regulator of Eg5 in promoting microtubule polymerization. Moreover, we further demonstrated that B23 regulates microtubule dynamics by directly inhibiting Eg5 ATPase activity.
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
authors.forename: Guoxing
authors.initials: G
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Yun
authors.initials: Y
authors.lastname: Huang
authors.forename: Zhan
authors.initials: Z
authors.lastname: Yao
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Shi
authors.forename: Mian
authors.initials: M
authors.lastname: Wu
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D034741:RNA, Small Interfering / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D013552:Swine; D020798:Two-Hybrid System Techniques
chemical_list: C470243:KIF11 protein, human; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D000090243:Nucleophosmin; D000251:Adenosine Triphosphatases; D016547:Kinesins
references.citation: Cancer Res. 2006 Feb 15;66(4):2059-66
references.pmid: 16489005
references.citation: Curr Biol. 2002 Jan 8;12(1):1-11
references.pmid: 11790298
references.citation: Biosci Biotechnol Biochem. 2002 Oct;66(10):2239-42
references.pmid: 12450141
references.citation: EMBO J. 2007 Feb 21;26(4):1068-80
references.pmid: 17290218
references.citation: Science. 2007 Apr 6;316(5821):120-3
references.pmid: 17412962
references.citation: Nat Cell Biol. 2005 Aug;7(8):823-30
references.pmid: 16041368
references.citation: J Biol Chem. 2001 Jun 15;276(24):21529-37
references.pmid: 11278991
references.citation: Cell. 2000 Sep 29;103(1):127-40
references.pmid: 11051553
references.citation: Cell. 2009 Jan 9;136(1):110-22
references.pmid: 19135893
references.citation: Cold Spring Harb Symp Quant Biol. 1982;46 Pt 1:483-93
references.pmid: 6955089
references.citation: J Cell Biochem. 2003 Dec 15;90(6):1140-8
references.pmid: 14635188
references.citation: Trends Biochem Sci. 1996 Jun;21(6):197-8
references.pmid: 8744350
references.citation: Blood. 2003 Jan 1;101(1):289-91
references.pmid: 12393441
references.citation: Int J Cancer. 1999 Jun 11;81(6):923-9
references.pmid: 10362140
references.citation: Cell Cycle. 2005 Nov;4(11):1593-8
references.pmid: 16205118
references.citation: Cell. 1999 Jan 8;96(1):69-78
references.pmid: 9989498
references.citation: Mol Cell Biol. 2005 Feb;25(4):1258-71
references.pmid: 15684379
references.citation: Nat Rev Cancer. 2006 Jul;6(7):493-505
references.pmid: 16794633
references.citation: Nat Cell Biol. 2006 Sep;8(9):957-62
references.pmid: 16906145
references.citation: J Biol Chem. 1991 Feb 25;266(6):3987-94
references.pmid: 1671676
references.citation: Cell. 2008 Nov 28;135(5):894-906
references.pmid: 19041752
references.citation: J Biol Chem. 2009 Oct 9;284(41):28410-28419
references.pmid: 19661056
references.citation: J Cell Biol. 2007 Sep 10;178(6):1081-91
references.pmid: 17846176
references.citation: Biochem Biophys Res Commun. 1999 Apr 21;257(3):865-70
references.pmid: 10208875
references.citation: J Biol Chem. 2009 Nov 13;284(46):31992-2001
references.pmid: 19759393
references.citation: Dev Cell. 2009 Oct;17(4):437-8
references.pmid: 19853556
references.citation: Oncogene. 2002 Sep 9;21(40):6170-4
references.pmid: 12214246
references.citation: J Cell Biol. 1988 Oct;107(4):1437-48
references.pmid: 3170635
references.citation: Blood. 2005 Aug 1;106(3):899-902
references.pmid: 15831697
references.citation: Nature. 2005 Oct 13;437(7061):1038-42
references.pmid: 16222248
references.citation: Cell. 1996 Jun 28;85(7):943-6
references.pmid: 8674121
references.citation: Biophys J. 1997 Mar;72(3):1357-75
references.pmid: 9138581
references.citation: Cell. 1989 Feb 10;56(3):379-90
references.pmid: 2914325