title: A novel approach to rapidly explore analytical markers for quality control of Radix Salviae Miltiorrhizae extract granules by robust principal component analysis with ultra-high performance liquid chromatography-ultraviolet-quadrupole time-of-flight mass spectrometry.
abstract: In a well-controlled experiment, outliers discriminated by robust principal component analysis (RPCA) represent contents in samples which are of particular quality distinguishable from the rest of the others, therefore chemical constituents in a natural product causing discrimination between outliers and the majority of samples could be considered as analytical markers for quality control. Based on this strategy, a novel approach for rapidly exploring characteristic analytical markers was proposed for the quality control of extract granules of Radix Salviae Miltiorrhizae (EGRSM). In this study, large sizes of samples were analyzed via high-throughput ultra-high performance liquid chromatography-ultraviolet-quadrupole time-of-flight mass spectrometry (UHPLC-UV-Q-Tof MS). RPCA was first performed on the three groups of samples: RSM (the raw material), the in-house prepared aqueous extract of Radix Salviae Miltiorrhizae (AERSM) and commercial product of EGRSM, to determine the variation of specific constituents between raw material and the final products as well as the effect of manufacturing process on the overall quality. Then RPCA was performed on the commercial products of EGRSM to explore the applicability of identified characteristic markers for the quality control of EGRSM. Candidate markers were extracted by RPCA, and their molecular formulae were determined by high resolution electrospray ionization-mass spectrometric (ESI-MS) analysis. The suitability of identified markers was then evaluated by determining the relationship between quantities of the identified markers with their antioxidant activities biologically, and further confirmed in a variety of samples. In conclusion, the combination of RPCA with UHPLC-UV-Q-Tof MS is a reliable means to identify chemical markers for evaluating quality of herbal medicines.
authors.affiliation: Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Shatin, NT, Hong Kong, China.
authors.forename: Jing-Zheng
authors.initials: JZ
authors.lastname: Song
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Qiao
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Chen
authors.forename: Hong-Xi
authors.initials: HX
authors.lastname: Xu
mesh_terms: D000975:Antioxidants / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057166:High-Throughput Screening Assays; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis*; D025341:Principal Component Analysis / Q000379:methods*; D011786:Quality Control; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts