title: The absence of inorganic salt is required for the crystallization of the complete oligomerization domain of Salmonella typhimurium histone-like nucleoid-structuring protein.
abstract: The histone-like nucleoid-structuring protein (H-NS) plays an important role in both DNA packaging and global gene regulation in enterobacteria. Self-association of the N-terminal domain results in polydisperse oligomers that are critical to the function of the protein. This heterogeneity in oligomer size has so far prevented structure determination of the complete oligomerization domain by NMR or X-ray crystallography. In the absence of inorganic salt, the H-NS oligomerization domain is predominantly restricted to an equilibrium between a homodimer and homotetramer, allowing a protein solution to be prepared that is sufficiently homogeneous for successful crystallization. Crystallization was achieved by tailoring the conditions screened to those identified as minimizing the potential disruption of protein-solution homogeneity. This finding provides a significant step towards resolving the structure of this important prokaryotic protein.
authors.affiliation: Department of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, England.
authors.forename: Paul G
authors.initials: PG
authors.lastname: Leonard
authors.forename: Gary N
authors.initials: GN
authors.lastname: Parkinson
authors.forename: Jayesh
authors.initials: J
authors.lastname: Gor
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Perkins
authors.forename: John E
authors.initials: JE
authors.lastname: Ladbury
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D006657:Histones / Q000737:chemistry* / Q000378:metabolism; D009994:Osmolar Concentration; D055503:Protein Multimerization; D012486:Salmonella typhimurium / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006657:Histones
references.citation: Mol Microbiol. 2007 Nov;66(3):727-43
references.pmid: 17908208
references.citation: Curr Opin Microbiol. 2004 Apr;7(2):109-14
references.pmid: 15063845
references.citation: Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1173-80
references.pmid: 16131749
references.citation: Protein Sci. 2003 Sep;12(9):1865-71
references.pmid: 12930986
references.citation: J Bacteriol. 2002 Feb;184(3):629-35
references.pmid: 11790731
references.citation: Mol Microbiol. 2001 Oct;42(2):439-52
references.pmid: 11703666
references.citation: Mol Microbiol. 2001 Apr;40(1):20-36
references.pmid: 11298273
references.citation: Mol Microbiol. 2009 Jul;73(2):165-79
references.pmid: 19508284
references.citation: J Mol Biol. 1968 Apr 28;33(2):491-7
references.pmid: 5700707
references.citation: Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82
references.pmid: 16369096
references.citation: FEBS Lett. 2002 Oct 9;529(2-3):151-6
references.pmid: 12372591
references.citation: Biophys J. 2000 Mar;78(3):1606-19
references.pmid: 10692345
references.citation: Acta Crystallogr D Biol Crystallogr. 2001 May;57(Pt 5):763-5
references.pmid: 11320330
references.citation: J Bacteriol. 2001 Apr;183(7):2343-7
references.pmid: 11244076
references.citation: Trends Microbiol. 1999 Mar;7(3):124-8
references.pmid: 10203842
references.citation: Nat Struct Biol. 2003 Mar;10(3):212-8
references.pmid: 12592399
references.citation: Biochem J. 2005 Oct 15;391(Pt 2):203-13
references.pmid: 15966862
references.citation: Nat Rev Microbiol. 2004 May;2(5):391-400
references.pmid: 15100692
references.citation: Mol Microbiol. 2000 Sep;37(5):1258-69
references.pmid: 10972841
references.citation: Mol Microbiol. 2005 Feb;55(3):808-27
references.pmid: 15661006
references.citation: J Mol Biol. 2002 Dec 6;324(4):841-50
references.pmid: 12460581
references.citation: J Bacteriol. 2009 Feb;191(3):1106-10
references.pmid: 19011022
references.citation: Acta Crystallogr D Biol Crystallogr. 1999 Oct;55(Pt 10):1718-25
references.pmid: 10531521
references.citation: Mol Microbiol. 2000 May;36(4):962-72
references.pmid: 10844682