title: Isolation of bovine herpesvirus type 5 from the semen of a healthy bull in Australia.
abstract: Artificial insemination is widely used in the cattle industry and a major challenge is to ensure that semen is free of infectious agents. A healthy donor bull was tested for freedom from infectious agents. A bovine herpesvirus was isolated in testis cells and identified as bovine herpesvirus type 5 (BoHV-5) by polymerase chain reaction and by direct amplicon sequencing. The amplicon sequence shared 100% similarity with the published sequence of BoHV-5. This is the first report in Australia of BoHV-5 in semen. The implications of this finding are discussed.
authors.affiliation: Biosecurity Sciences Laboratory, Department of Primary Industries and Fisheries, Locked Mail Bag 4, Moorooka, Queensland 4105, Australia. Ibrahim.diallo@dpi.qld.gov.au
authors.forename: I S
authors.initials: IS
authors.lastname: Diallo
authors.forename: G R
authors.initials: GR
authors.lastname: Hewitson
authors.forename: J
authors.initials: J
authors.lastname: Hoad
authors.forename: S
authors.initials: S
authors.lastname: Turner
authors.forename: B G
authors.initials: BG
authors.lastname: Corney
authors.forename: B J
authors.initials: BJ
authors.lastname: Rodwell
mesh_terms: D000818:Animals; D001315:Australia; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000635:transmission / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D018792:Encephalitis, Viral / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D025881:Herpesvirus 5, Bovine / Q000302:isolation & purification*; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D008590:Meningoencephalitis / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012661:Semen / Q000821:virology*
chemical_list: D004279:DNA, Viral