title: Using buoyant mass to measure the growth of single cells.
abstract: We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.
authors.affiliation: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, USA.
authors.forename: Michel
authors.initials: M
authors.lastname: Godin
authors.forename: Francisco Feijó
authors.initials: FF
authors.lastname: Delgado
authors.forename: Sungmin
authors.initials: S
authors.lastname: Son
authors.forename: William H
authors.initials: WH
authors.lastname: Grover
authors.forename: Andrea K
authors.initials: AK
authors.lastname: Bryan
authors.forename: Amit
authors.initials: A
authors.lastname: Tzur
authors.forename: Paul
authors.initials: P
authors.lastname: Jorgensen
authors.forename: Kris
authors.initials: K
authors.lastname: Payer
authors.forename: Alan D
authors.initials: AD
authors.lastname: Grossman
authors.forename: Marc W
authors.initials: MW
authors.lastname: Kirschner
authors.forename: Scott R
authors.initials: SR
authors.lastname: Manalis
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000254:growth & development; D002453:Cell Cycle; D049108:Cell Enlargement; D004926:Escherichia coli / Q000254:growth & development; D008214:Lymphocytes / Q000502:physiology; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000254:growth & development
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