title: Expressional and functional studies of CKLF1 during dendritic cell maturation.
abstract: Chemokine-like factor 1 (CKLF1) was the first member of the CKLF-like MARVEL transmembrane domain containing member (CMTM) family to be discovered. Its expression level is increased clearly in peripheral blood lymphocytes upon phytohemagglutinin stimulation, but little is known about the expression and function of CKLF1 in dendritic cells (DCs), which are the most potent antigen-presenting cells. In the present study, we showed that CKLF1 was highly expressed in monocytes. During differentiation from monocytes to immature DCs, CKLF1 was increased dramatically on day 2 and then decreased from day 3 to 5. Upon maturation with different stimuli, CKLF1 was down-regulated. Two peptides of CKLF1, C19 and C27, stimulated the effect of immature DCs (imDCs) on T-cell proliferation and IFN-gamma production. Further study on DC maturation showed that C19 and C27 up-regulated HLA-DR expression and IL-12 secretion, with no obvious effects on CD80, CD83 or CD86. Thus, CKLF1-C19 and -C27 can stimulate antigen-presenting capability of imDCs.
authors.affiliation: Center for Human Disease Genomics, Department of Immunology, School of Basic Medical Sciences, Peking University, China.
authors.forename: Luning
authors.initials: L
authors.lastname: Shao
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Mo
authors.forename: Otto
authors.initials: O
authors.lastname: Majdic
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Maria
authors.initials: M
authors.lastname: Seyerl
authors.forename: Catharina
authors.initials: C
authors.lastname: Schrauf
authors.forename: Dalong
authors.initials: D
authors.lastname: Ma
authors.forename: Johannes
authors.initials: J
authors.lastname: Stöckl
authors.forename: Wenling
authors.initials: W
authors.lastname: Han
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D018925:Chemokines / Q000378:metabolism* / Q000494:pharmacology; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology*; D015536:Down-Regulation; D005786:Gene Expression Regulation; D006801:Humans; D018664:Interleukin-12 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D062792:MARVEL Domain-Containing Proteins
chemical_list: C432676:CKLF protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins; D018664:Interleukin-12