Trichostatin A inhibits expression of cathepsins in experimental osteoarthritis.
The aim of this study was to investigate the effects of trichostatin A (TSA) on expression of cathepsins in cartilage in experimental osteoarthritis (OA). OA was induced in 18 rabbits by bilateral anterior cruciate ligament transection (ACLT). Four weeks after surgery, rabbits received intra-articular injection with TSA dissolved in the dimethylsulphoxide (DMSO) in the right knees and DMSO in the left knees once a week for 5 weeks. Rabbits were killed 7 days after the last injection. The knee joints were assessed by morphological and histological examination. Messenger RNA expression of cathepsins K, B, L, S and cystatin C was studied by real-time PCR. TSA inhibited the expression of cathepsins K, B, L, S and cystatin C accompanied with the less degradation in cartilage. The results suggest that TSA exhibits protective effects against cartilage degradation in rabbits with OA and the effects may be associated with the inhibition of cathepsins.
Chen
Wei-Ping
WP
Department of Orthopedics Surgery, The Second Hospital of Medical College, Zhejiang University, Jie Fang Road 88#, 310009 Hangzhou, People’s Republic of China.
Bao
Jia-Peng
JP
Tang
Jin-Li
JL
Hu
Peng-Fei
PF
Wu
Li-Dong
LD
D000818:Animals; D002403:Cathepsins / Q000037:antagonists & inhibitors* / Q000235:genetics*; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008297:Male; D020370:Osteoarthritis, Knee / Q000188:drug therapy* / Q000201:enzymology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D011817:Rabbits
D006877:Hydroxamic Acids; D011480:Protease Inhibitors; C012589:trichostatin A; D002403:Cathepsins
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