Photoacoustic ophthalmoscopy for in vivo retinal imaging.
We have developed a non-invasive photoacoustic ophthalmoscopy (PAOM) for in vivo retinal imaging. PAOM detects the photoacoustic signal induced by pulsed laser light shined onto the retina. By using a stationary ultrasonic transducer in contact with the eyelids and scanning only the laser light across the retina, PAOM provides volumetric imaging of the retinal micro-vasculature and retinal pigment epithelium at a high speed. For B-scan frames containing 256 A-lines, the current PAOM has a frame rate of 93 Hz, which is comparable with state-of-the-art commercial spectral-domain optical coherence tomography (SD-OCT). By integrating PAOM with SD-OCT, we further achieved OCT-guided PAOM, which can provide multi-modal retinal imaging simultaneously. The capabilities of this novel technology were demonstrated by imaging both the microanatomy and microvasculature of the rat retina in vivo.
Jiao
Shuliang
S
Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
Jiang
Minshan
M
Hu
Jianming
J
Fawzi
Amani
A
Zhou
Qifa
Q
Shung
K Kirk
KK
Puliafito
Carmen A
CA
Zhang
Hao F
HF
D000162:Acoustics / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D012160:Retina / Q000033:anatomy & histology*; D042261:Retinoscopes
Opt Lett. 2009 Jun 15;34(12):1771-3
19529698
Opt Lett. 2009 Oct 1;34(19):2961-3
19794782
Nat Biotechnol. 2006 Jul;24(7):848-51
16823374
Arch Ophthalmol. 1977 May;95(5):861-8
860947
Opt Express. 2004 Feb 9;12(3):367-76
19474832
Opt Express. 2010 Jan 18;18(2):1278-82
20173952
J Biomed Opt. 2009 Mar-Apr;14(2):024050
19405778
Opt Lett. 2008 May 1;33(9):929-31
18451942
Opt Express. 2005 Jan 24;13(2):444-52
19488371
Ophthalmic Surg Lasers Imaging. 2003 Mar-Apr;34(2):152-64
12665234
Invest Ophthalmol Vis Sci. 2007 Apr;48(4):1808-14
17389515
Eye (Lond). 2002 May;16(3):242-60
12032713
Br J Ophthalmol. 1995 May;79(5):407-12
7612549
J Biomed Opt. 2002 Jul;7(3):457-63
12175297
Nat Protoc. 2007;2(4):797-804
17446879
Science. 1991 Nov 22;254(5035):1178-81
1957169
IEEE Trans Biomed Eng. 1981 Jul;28(7):488-92
7275128
Ophthalmology. 2004 May;111(5):1055-62
15121388
IEEE Trans Biomed Eng. 2001 May;48(5):592-8
11341533
Physiol Rev. 2005 Jul;85(3):845-81
15987797