Lactobacillus jensenii surface-associated proteins inhibit Neisseria gonorrhoeae adherence to epithelial cells.
High numbers of lactobacilli in the vaginal tract have been correlated with a decreased risk of infection by the sexually transmitted pathogen Neisseria gonorrhoeae. We have previously shown that Lactobacillus jensenii, one of the most prevalent microorganisms in the healthy human vaginal tract, can inhibit gonococcal adherence to epithelial cells in culture. Here we examined the role of the epithelial cells and the components of L. jensenii involved in the inhibition of gonococcal adherence. L. jensenii inhibited the adherence of gonococci to glutaraldehyde-fixed epithelial cells like it inhibited the adherence of gonococci to live epithelial cells, suggesting that the epithelial cells do not need to be metabolically active for the inhibition to occur. In addition, methanol-fixed L. jensenii inhibited gonococcal adherence to live epithelial cells, indicating that L. jensenii uses a constitutive component to inhibit gonococcal interactions with epithelial cells. Proteinase K treatment of methanol-fixed lactobacilli eliminated the inhibitory effect, suggesting that the inhibitory component contains protein. Released surface components (RSC) isolated from L. jensenii were found to contain at least two inhibitory components, both of which are protease sensitive. Using anion-exchange and size exclusion chromatography, an inhibitory protein which exhibits significant similarity to the enzyme enolase was isolated. A recombinant His6-tagged version of this protein was subsequently produced and shown to inhibit gonococcal adherence to epithelial cells in a dose-dependent manner.
Spurbeck
Rachel R
RR
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824-1101, USA.
Arvidson
Cindy Grove
CG
D001422:Bacterial Adhesion / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology*; D002460:Cell Line; D002852:Chromatography, Ion Exchange; D004717:Endometrium / Q000382:microbiology; D004848:Epithelium / Q000382:microbiology*; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D006069:Gonorrhea / Q000382:microbiology*; D006801:Humans; D007778:Lactobacillus / Q000235:genetics / Q000502:physiology*; D009344:Neisseria gonorrhoeae / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry
D001425:Bacterial Outer Membrane Proteins; D010751:Phosphopyruvate Hydratase
Cell Microbiol. 2007 Feb;9(2):356-67
16925785
J Appl Microbiol. 2008 Jun;104(6):1667-74
18194256
FEMS Immunol Med Microbiol. 2006 Dec;48(3):424-32
17059467
ISME J. 2007 Jun;1(2):121-33
18043622
FEMS Immunol Med Microbiol. 2007 Dec;51(3):526-34
17892475
Environ Microbiol. 2001 Apr;3(4):229-36
11359508
Clin Infect Dis. 2003 Mar 1;36(5):663-8
12594649
Scand J Urol Nephrol Suppl. 1984;86:13-22
6399405
Curr Microbiol. 2000 Aug;41(2):136-41
10856380
Infect Immun. 2008 Jul;76(7):3124-30
18411284
J Appl Microbiol. 1997 Oct;83(4):413-20
9351223
Am J Clin Nutr. 2001 Feb;73(2 Suppl):437S-443S
11157354
Infect Immun. 2008 Apr;76(4):1340-8
18227169
Contraception. 2006 Jan;73(1):78-81
16371300
J Infect Dis. 2002 May 1;185(9):1369-72
12001060
Mol Microbiol. 2006 Jan;59(2):528-40
16390447
Microb Cell Fact. 2009 Feb 16;8:14
19220903
AIDS. 2008 Sep 12;22(14):1729-37
18753933
J Bacteriol. 2007 Jun;189(12):4539-43
17449624
J Mol Microbiol Biotechnol. 2009;17(3):158-62
19672048
J Exp Med. 1987 May 1;165(5):1344-57
3106555
J Microbiol Biotechnol. 2008 Jul;18(7):1278-85
18667857
Am J Obstet Gynecol. 2001 Aug;185(2):375-9
11518895
Mol Microbiol. 1998 Jul;29(1):369-79
9701828
Infect Immun. 1985 Jan;47(1):84-9
3917428
World J Urol. 2000 Dec;18(6):422-6
11204262
Appl Environ Microbiol. 2007 Jul;73(13):4259-67
17496132
Microbes Infect. 2008 Oct;10(12-13):1325-34
18761100
J Exp Med. 1973 Mar 1;137(3):571-89
4631989
World J Gastroenterol. 2007 Apr 7;13(13):1962-5
17461497
Folia Microbiol (Praha). 2008;53(1):61-6
18481220
J Infect Dis. 1981 Mar;143(3):413-22
6785363
Mol Med. 1996 Nov;2(6):745-54
8972489
J Infect Dis. 2005 Jan 1;191(1):25-32
15592999
Infect Immun. 1998 May;66(5):1985-9
9573080
Appl Environ Microbiol. 1996 Jun;62(6):1958-63
8787394
MMWR Morb Mortal Wkly Rep. 2007 Apr 13;56(14):332-6
17431378
Cell. 1985 Feb;40(2):293-300
2857113
Infect Immun. 1997 Sep;65(9):3736-42
9284146
Infect Immun. 1999 Oct;67(10):5170-5
10496892
FEMS Microbiol Lett. 2000 Sep 1;190(1):177-80
10981710
BMC Microbiol. 2009 Mar 31;9:63
19331693
FEMS Immunol Med Microbiol. 1997 Oct;19(2):111-4
9395055
J Appl Microbiol. 2002;92(3):451-9
11872120
J Bacteriol. 1968 Sep;96(3):596-605
4979098
Protein Expr Purif. 2005 May;41(1):207-34
15915565
Methods Enzymol. 1999;310:426-33
10547809
J Bacteriol. 1963 Jun;85:1274-9
14047217