title: Comprehensive miRNA profiling of surgically staged endometrial cancer.
abstract: OBJECTIVE
  We sought to determine a microRNA (miRNA) profile of surgically staged endometrial cancers.
  
  
  METHODS
  RNA was extracted from archival primary endometrial cancers, and an miRNA profile was established using a microarray and confirmed with real-time polymerase chain reaction. Targets of differentially expressed miRNAs were explored using real-time polymerase chain reaction and Western blot in endometrial cell lines.
  
  
  RESULTS
  Endometrial cancer has an miRNA profile distinct from normal endometrium, even in patients with stage IA grade 1 tumors. This miRNA cancer profile was able to correctly assign a specimen as a malignancy with a sensitivity of 92%. Overexpressed miRNAs were predicted to target PTEN, and transfection of cell lines with these miRNAs led to down-regulation of PTEN expression. In advanced disease, an miRNA pattern distinct from early-stage disease was seen, and overexpression of mir-199c predicted improved cancer survival in this population.
  
  
  CONCLUSIONS
  Endometrial cancer has a distinct miRNA profile, and miRNAs can be used as a predictive biomarker.
authors.affiliation: Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, The Ohio State University College of Medicine, Columbus, OH, USA. David.Cohn@osumc.edu
authors.forename: David E
authors.initials: DE
authors.lastname: Cohn
authors.forename: Muller
authors.initials: M
authors.lastname: Fabbri
authors.forename: Nicola
authors.initials: N
authors.lastname: Valeri
authors.forename: Hansjuerg
authors.initials: H
authors.lastname: Alder
authors.forename: Iouri
authors.initials: I
authors.lastname: Ivanov
authors.forename: Chang-Gong
authors.initials: CG
authors.lastname: Liu
authors.forename: Carlo M
authors.initials: CM
authors.lastname: Croce
authors.forename: Kimberly E
authors.initials: KE
authors.lastname: Resnick
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009367:Neoplasm Staging; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
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