Intravital investigation of the effects of serotonin and glutamate on the dynamics of DNA activity in L-RPl1 neurons of edible snail.
Microloci of varying size with increased fluorescence were observed in L-RPl1 neuronal nuclei of snails using DNA-selective dye SYTO16. Application of serotonin and glutamate increased the number of small (<1 micro in diameter) microloci, while the number of medium and large (1-3 micro) loci decreased. Combined application of neurotransmitters produced more pronounced changes in the number of microloci compared to individual administration. RNA synthesis inhibitors abolished the effects of the transmitters. We hypothesized that the revealed small microloci of fluorescence are "active" DNA zones, where transcription of new genes are initiated.
Shevelkin
A V
AV
Laboratory of Functional Neurochemistry, P. K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia. shevelkin_a@mail.ru
Nikitin
V P
VP
Sherstnev
V V
VV
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D018698:Glutamic Acid / Q000494:pharmacology*; D006372:Helix, Snails / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology*; D018490:Serotonin Agents / Q000494:pharmacology
D002843:Chromatin; D005456:Fluorescent Dyes; D018490:Serotonin Agents; D012701:Serotonin; D018698:Glutamic Acid; D004247:DNA