A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus.
Mycoviruses are viruses that infect fungi and have the potential to control fungal diseases of crops when associated with hypovirulence. Typically, mycoviruses have double-stranded (ds) or single-stranded (ss) RNA genomes. No mycoviruses with DNA genomes have previously been reported. Here, we describe a hypovirulence-associated circular ssDNA mycovirus from the plant pathogenic fungus Sclerotinia sclerotiorum. The genome of this ssDNA virus, named Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), is 2166 nt, coding for a replication initiation protein (Rep) and a coat protein (CP). Although phylogenetic analysis of Rep showed that SsHADV-1 is related to geminiviruses, it is notably distinct from geminiviruses both in genome organization and particle morphology. Polyethylene glycol-mediated transfection of fungal protoplasts was successful with either purified SsHADV-1 particles or viral DNA isolated directly from infected mycelium. The discovery of an ssDNA mycovirus enhances the potential of exploring fungal viruses as valuable tools for molecular manipulation of fungi and for plant disease control and expands our knowledge of global virus ecology and evolution.
Yu
Xiao
X
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China.
Li
Bo
B
Fu
Yanping
Y
Jiang
Daohong
D
Ghabrial
Said A
SA
Li
Guoqing
G
Peng
Youliang
Y
Xie
Jiatao
J
Cheng
Jiasen
J
Huang
Junbin
J
Yi
Xianhong
X
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics / Q000472:pathogenicity* / Q000821:virology*; D001483:Base Sequence; D017124:Conserved Sequence; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D017791:Geminiviridae / Q000235:genetics / Q000472:pathogenicity* / Q000648:ultrastructure; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D016415:Sequence Alignment; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics; D014771:Virion / Q000648:ultrastructure; D014774:Virulence
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases
J Virol. 2004 Jan;78(2):892-8
14694120
BMC Evol Biol. 2009 May 21;9:112
19460138
Virology. 1983 Oct 15;130(1):21-8
18639136
Nucleic Acids Res. 1995 Mar 25;23(6):910-6
7731803
J Gen Virol. 2006 Jan;87(Pt 1):241-249
16361437
Trends Plant Sci. 2006 May;11(5):209-12
16616578
Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3879-83
7732000
Trends Plant Sci. 2003 Mar;8(3):128-34
12663223
Annu Rev Phytopathol. 2005;43:361-94
16078889
Proc Biol Sci. 2002 Nov 7;269(1506):2269-76
12455515
Nat Rev Microbiol. 2005 Aug;3(8):632-42
16064055
Virology. 1992 Apr;187(2):633-42
1546458
Methods Mol Biol. 1998;81:41-52
9760492
J Virol. 2004 Mar;78(5):2405-13
14963136
FEBS Lett. 2009 Jun 18;583(12):1825-32
19497325
Plant Biotechnol J. 2009 Apr;7(3):254-65
19175519
Microbiol Mol Biol Rev. 2000 Sep;64(3):489-502
10974123
Science. 1982 Jan 29;215(4532):466-71
17771259
Curr Opin Plant Biol. 2006 Apr;9(2):209-15
16480918
Science. 2004 Apr 2;304(5667):66-74
15001713
Virol J. 2009 Jul 07;6:96
19583873
Arch Virol. 2008;153(8):1441-51
18566736
Science. 2007 Mar 30;315(5820):1786-7
17395806
Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):7088-93
9192696
Nucleic Acids Res. 1985 Apr 11;13(7):2189-206
4000956
FEMS Microbiol Lett. 2005 Feb 15;243(2):323-9
15686831
Plant Methods. 2009 Jul 02;5:9
19573239
Genetics. 2001 Sep;159(1):107-18
11560890
Mol Plant Microbe Interact. 2003 Sep;16(9):785-95
12971602
J Gen Virol. 1997 Aug;78 ( Pt 8):2113-7
9267015
Science. 1992 Aug 7;257(5071):800-3
1496400
Methods Enzymol. 1996;266:131-41
8743682
J Virol. 2009 Feb;83(4):1981-91
19073734