Oxidation changes physical properties of phospholipid bilayers: fluorescence spectroscopy and molecular simulations.
Physical properties of oxidized phospholipid (OxPL) membranes consisting of binary mixtures of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 10 mol % of one of two OxPLs, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC) or 1-palmitoyl-2-(5'-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC), were investigated experimentally and computationally. Fluorescence solvent relaxation (SR) and fluorescence correlation spectroscopy z-scan (FCS z-scan) show increased headgroup hydration and mobility, and faster lateral diffusion in POPC membrane upon addition of OxPLs. The magnitudes of both effects are distinct for each of the two OxPLs. Molecular dynamics simulations corroborate the experimental findings, providing at the same time a detailed molecular interpretation in terms of changes in bilayer structure and phospholipid orientation.
Beranova
Lenka
L
J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v. v. i., Dolejskova 3, 18223 Prague 8, Czech Republic.
Cwiklik
Lukasz
L
Jurkiewicz
Piotr
P
Hof
Martin
M
Jungwirth
Pavel
P
D002462:Cell Membrane / Q000737:chemistry; D004058:Diffusion; D008051:Lipid Bilayers / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000737:chemistry; D010742:Phospholipid Ethers / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D055585:Physical Phenomena; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence
C400184:1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphorylcholine; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010742:Phospholipid Ethers; D010743:Phospholipids; D012997:Solvents; C028694:1-palmitoyl-2-oleoylphosphatidylcholine