First description of the adult stage of Clinostomum cutaneum Paperna, 1964 (Digenea: Clinostomidae) from grey herons Ardea cinerea L. and a redescription of the metacercaria from the Nile tilapia Oreochromis niloticus niloticus (L.) in Kenya.
The combined use of morphological and molecular studies allowed for the first time the recognition and description of the adult stage of Clinostomum cutaneum Paperna, 1964 from the grey heron Ardea cinerea L. in Kenya. A redescription of the metacercaria that infect Nile tilapia Oreochromis niloticus niloticus (L.) from the same aquatic environment is also presented. C. cutaneum differs from all other species of Clinostomum Leidy, 1856 in the shape of its uterus. Sequencing the rRNA confirmed the morphological similarity between adults from the grey heron and the metacercarial stage from tilapia, and a level of genetic similarity with the other previously sequenced Clinostomum spp. was observed. The need for a reorganisation of Clinostomum using both morphological and molecular methods is highlighted.
Gustinelli
Andrea
A
Department of Veterinary Public Health and Animal Pathology, University of Bologna, via Tolara di Sopra 50, 40064, Ozzano Emilia, BO, Italy. andrea.gustinelli2@unibo.it
Caffara
Monica
M
Florio
Daniela
D
Otachi
Elick O
EO
Wathuta
Euty M
EM
Fioravanti
Maria L
ML
D000818:Animals; D001717:Birds / Q000469:parasitology*; D023681:Cichlids / Q000469:parasitology*; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D007630:Kenya; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014200:Trematoda / Q000033:anatomy & histology / Q000145:classification* / Q000302:isolation & purification*
D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S
J Helminthol. 1966;40(1):187-214
6006729
Nucleic Acids Res. 1992 Nov 25;20(22):6101-2
1461743
Mol Biochem Parasitol. 1994 Jan;63(1):153-6
8183315
Parasitology. 1998 Apr;116 ( Pt 4):311-7
9585933
Folia Parasitol (Praha). 2004 Dec;51(4):320-6
15729944
Mol Biochem Parasitol. 1996 Jan;75(2):265-9
8992325
Parasitology. 1995 Dec;111 ( Pt 5):609-15
8559594
Trends Genet. 2003 Jul;19(7):370-5
12850441
Parasitology. 2002 Mar;124(Pt 3):307-13
11922432
Int J Parasitol. 2003 Jul;33(7):733-55
12814653
J Mol Evol. 1991 Mar;32(3):253-61
1904502
Int J Parasitol. 1998 Nov;28(11):1791-5
9846617
Parasitology. 2003 Mar;126(Pt 3):203-24
12666879
Mol Biol Evol. 1996 Nov;13(9):1276-95
8896380
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712
Q Rev Biol. 1991 Dec;66(4):411-53
1784710
Mol Biochem Parasitol. 1992 Dec;56(2):323-7
1484553
J Parasitol. 1998 Feb;84(1):114-24
9488348
J Parasitol. 2004 Apr;90(2):413-4
15165071
Parasitol Res. 2002 Mar;88(3):230-41
11954908
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
7984417
Adv Parasitol. 2005;60:101-63
16230103