Measuring cilium-induced Ca2+ increases in cultured renal epithelia.
The primary cilium is a sensory organelle in many mammalian cells. Functional analysis has established the cilium to be instrumental in the detection of mechanical and environmental changes. The cilium detects these stimuli and activates various signal-transduction pathways including alteration of intracellular Ca(2+) concentration ([Ca(2+)](i)). This chapter describes methods of measuring primary cilium-dependent [Ca(2+)](i) signaling.
Praetorius
Helle A
HA
Department of Physiology and Biophysics, Aarhus University, 8000 Aarhus C, Denmark.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002478:Cells, Cultured; D002923:Cilia / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D007668:Kidney / Q000166:cytology*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D015398:Signal Transduction
D002118:Calcium