Functional surfaces for high-resolution analysis of cancer cell interactions on exogenous hyaluronic acid.
Hyaluronic acid, a nonsulfated, linear glycosaminoglycan, is ubiquitously distributed in the extracellular matrix and is known to facilitate tumor progression by enhancing invasion, growth, and angiogenesis. Native HA has been attached to substrates to create patterned surfaces resistant to cell adhesion, and has been utilized in a variety of cell adhesion studies using either non covalently bound layers patterned by soft lithography or related methods. We use a new approach to study cell interactions with HA-presenting regions, by covalently linking HA adjacent to PEG-ylated regions, which resist cell adhesion. Colon and breast cancer cells seeded on the patterned HA surfaces adhere preferentially on HA-presenting regions and proliferate there. Furthermore, we demonstrate that cell adhesion is inhibited with the blocking of HA receptor, CD44, and that cellular adhesive processes, through protrusions spreading onto the HA surface, enhance spreading and movement outside the HA-presenting regions. Overall, this approach allows high-resolution analysis of cancer cell attachment, growth, and migration on exogenous native HA.
Dickinson
Laura E
LE
Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Science Oncology Center and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Ho
Chia Chi
CC
Wang
Geoffrey M
GM
Stebe
Kathleen J
KJ
Gerecht
Sharon
S
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects*; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D006820:Hyaluronic Acid / Q000494:pharmacology*; D051379:Mice; D041681:NIH 3T3 Cells; D009369:Neoplasms / Q000473:pathology*; D013499:Surface Properties / Q000187:drug effects
D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid
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