title: A streptavidin variant with slower biotin dissociation and increased mechanostability.
abstract: Streptavidin binds biotin conjugates with exceptional stability but dissociation does occur, limiting its use in imaging, DNA amplification and nanotechnology. We identified a mutant streptavidin, traptavidin, with more than tenfold slower biotin dissociation, increased mechanical strength and improved thermostability; this resilience should enable diverse applications. FtsK, a motor protein important in chromosome segregation, rapidly displaced streptavidin from biotinylated DNA, whereas traptavidin resisted displacement, indicating the force generated by Ftsk translocation.
authors.affiliation: Department of Biochemistry, Oxford University, UK.
authors.forename: Claire E
authors.initials: CE
authors.lastname: Chivers
authors.forename: Estelle
authors.initials: E
authors.lastname: Crozat
authors.forename: Calvin
authors.initials: C
authors.lastname: Chu
authors.forename: Vincent T
authors.initials: VT
authors.lastname: Moy
authors.forename: David J
authors.initials: DJ
authors.lastname: Sherratt
authors.forename: Mark
authors.initials: M
authors.lastname: Howarth
mesh_terms: D001696:Biomechanical Phenomena; D001710:Biotin / Q000378:metabolism*; D019921:Biotinylation; D029968:Escherichia coli Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D011485:Protein Binding; D015202:Protein Engineering; D019809:Streptavidin / Q000235:genetics* / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; C096452:FtsK protein, E coli; D008565:Membrane Proteins; C549444:traptavidin; D001710:Biotin; D019809:Streptavidin
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