Computational protein design, from single domain soluble proteins to membrane proteins.
Computational protein design is becoming more and more important in both understanding the fundamental biological roles of proteins and facilitating their utilization in biotechnology. Based upon the significant progress in our understanding of protein folding, development of efficient sequence and conformational search algorithms and accurate scoring functions, and ever increasing computational resources, great progress has been made in design of single domain soluble proteins. There are also reports of membrane protein design, albeit very limited. This tutorial review will give a brief introduction, the state of the art, and future prospects and implications of protein design in improving healthcare and environment. The interplay between protein design, molecular simulations and experiments will be presented to emphasize the interdisciplinary nature of the field.
Tian
Pu
P
College of Life Sciences & Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, 2699 Qian-Jin, Street, Changchun, China 130012. tianpu@jlu.edu.cn
D019295:Computational Biology; D004798:Enzymes / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D013816:Thermodynamics
D004798:Enzymes; D008565:Membrane Proteins