title: Differential gene expression profiling on the muscle of acetylcholinesterase knockout mice: a preliminary analysis.
abstract: Acetylcholinesterase (AChE) (EC. 3.1.1.7) is the acetylcholine-hydrolyzing enzyme that plays an essential role on cholinergic neurotransmission at the synapses of the brain and at the neuromuscular junctions. In order to gain insight into the molecular mechanisms of neuromuscular dysfunction associated with AChE deficiency, we have compared the RNA expression profiles of the muscles of AChE knockout mice with those of the wild-type siblings. Total RNA from the leg muscle of the mice of the wild-type and the AChE nullizygous mice were subjected to microarray analyses with Affymetrix GeneChip((R)) Mouse Gene 1.0 ST Array. The pair-wise comparison of gene expression levels of the 28,853 mRNA transcripts showed that 303 genes were either up- or down-regulated by more than 2.0 folds in the AChE knockout mice. The interaction study of these differentially regulated genes indicated that some of these genes are clustered in biological functions that are related to lipid metabolism and the skeletal-muscular functions.
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China.
authors.forename: Huang-Quan
authors.initials: HQ
authors.lastname: Lin
authors.forename: Roy
authors.initials: R
authors.lastname: Choi
authors.forename: Kam-Leung
authors.initials: KL
authors.lastname: Chan
authors.forename: Denis
authors.initials: D
authors.lastname: Ip
authors.forename: Karl Wah-Keung
authors.initials: KW
authors.lastname: Tsim
authors.forename: David Chi-Cheong
authors.initials: DC
authors.lastname: Wan
mesh_terms: D000110:Acetylcholinesterase / Q000172:deficiency* / Q000235:genetics*; D000818:Animals; D020869:Gene Expression Profiling; D055786:Gene Knockout Techniques; D051379:Mice; D018345:Mice, Knockout; D009132:Muscles / Q000201:enzymology / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000201:enzymology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D035781:Siblings
chemical_list: D012333:RNA, Messenger; D000110:Acetylcholinesterase