Biotransformation of mulberroside A from Morus alba results in enhancement of tyrosinase inhibition.
Mulberroside A, a glycosylated stilbene, was isolated and identified from the ethanol extract of the roots of Morus alba. Oxyresveratrol, the aglycone of mulberroside A, was produced by enzymatic hydrolysis of mulberroside A using the commercial enzyme Pectinex. Mulberroside A and oxyresveratrol showed inhibitory activity against mushroom tyrosinase with an IC(50) of 53.6 and 0.49 microM, respectively. The tyrosinase inhibitory activity of oxyresveratrol was thus approximately 110-fold higher than that of mulberroside A. Inhibition kinetics showed mulberroside A to be a competitive inhibitor of mushroom tyrosinase with L-tyrosine and L-DOPA as substrate. Oxyresveratrol showed mixed inhibition and noncompetitive inhibition against L-tyrosine and L-DOPA, respectively, as substrate. The results indicate that the tyrosinase inhibitory activity of mulberroside A was greatly enhanced by the bioconversion process.
Kim
Jeong-Keun
JK
Department of Chemical Engineering and Biotechnology, Korea Polytechnic University, Shihung-si, Kyunggi-do 429-793, South Korea.
Kim
Mijin
M
Cho
Ssang-Goo
SG
Kim
Myung-Kyoo
MK
Kim
Suhng Wook
SW
Lim
Young-Hee
YH
D000363:Agaricales / Q000201:enzymology; D001711:Biotransformation; D004187:Disaccharides / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000378:metabolism* / Q000494:pharmacology; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D031625:Morus / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism; D013267:Stilbenes / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology
D004187:Disaccharides; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D013267:Stilbenes; C420606:mulberroside A; C034912:puag-haad; D014442:Monophenol Monooxygenase
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