Lysobacter ruishenii sp. nov., a chlorothalonil-degrading bacterium isolated from a long-term chlorothalonil-contaminated soil.
An aerobic, Gram-negative bacterial strain, designated CTN-1(T), capable of degrading chlorothalonil was isolated from a long-term chlorothalonil-contaminated soil in China, and was subjected to a polyphasic taxonomic investigation. Strain CTN-1(T) grew at 15-37 °C (optimum 28-30 °C) and at pH 6.0-9.0 (optimum pH 7.0-7.5). The G+C content of the total DNA was 67.1 mol%. Based on 16S rRNA gene sequence analysis, strain CTN-1(T) was related most closely to Lysobacter daejeonensis DSM 17634(T) (97.1  % similarity), L. soli DCY21(T) (95.7  %), L. concretionis Ko07(T) (95.5  %), L. gummosus LMG 8763(T) (95.3 %) and L. niastensis DSM 18481(T) (95.2  %). The novel strain showed less than 95.0 % 16S rRNA gene sequence similarity to the type strains of other Lysobacter species. The major cellular fatty acids of strain CNT-1(T) were iso-C₁₆:₀ (23.0  %), iso-C₁₅:₀ (21.4  %) and iso-C₁₇:₁ω9c (15.3  %). The major isoprenoid quinone was Q-8 (99 %), and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. These chemotaxonomic data supported the affiliation of strain CTN-1(T) to the genus Lysobacter. Levels of DNA-DNA relatedness between strain CTN-1(T) and L. daejeonensis DSM 17634(T) were 34.6-36.1  %. Phylogenetic analysis based on 16S rRNA gene sequences, DNA-DNA hybridization data and biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strain CTN-1(T) from recognized species of the genus Lysobacter. Strain CTN-1(T) is therefore considered to represent a novel species of the genus Lysobacter, for which the name Lysobacter ruishenii sp. nov. is proposed. The type strain is CTN-1(T) (=DSM 22393(T) =CGMCC 1.10136(T)).
Wang
Guang-Li
GL
Department of Microbiology, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, PR China.
Wang
Li
L
Department of Microbiology, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, PR China.
Chen
Hong-Hong
HH
Department of Microbiology, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, PR China.
Shen
Bin
B
Department of Microbiology, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, PR China.
Li
Shun-Peng
SP
Department of Microbiology, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, PR China.
Jiang
Jian-Dong
JD
Department of Microbiology, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, PR China.
D001482:Base Composition; D001711:Biotransformation; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D053528:Lysobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009570:Nitriles / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009570:Nitriles; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; C005806:tetrachloroisophthalonitrile