title: A cellulose synthase-like protein is required for osmotic stress tolerance in Arabidopsis.
abstract: Osmotic stress imposed by soil salinity and drought stress significantly affects plant growth and development, but osmotic stress sensing and tolerance mechanisms are not well understood. Forward genetic screens using a root-bending assay have previously identified salt overly sensitive (sos) mutants of Arabidopsis that fall into five loci, SOS1 to SOS5. These loci are required for the regulation of ion homeostasis or cell expansion under salt stress, but do not play a major role in plant tolerance to the osmotic stress component of soil salinity or drought. Here we report an additional sos mutant, sos6-1, which defines a locus essential for osmotic stress tolerance. sos6-1 plants are hypersensitive to salt stress and osmotic stress imposed by mannitol or polyethylene glycol in culture media or by water deficit in the soil. SOS6 encodes a cellulose synthase-like protein, AtCSLD5. Only modest differences in cell wall chemical composition could be detected, but we found that sos6-1 mutant plants accumulate high levels of reactive oxygen species (ROS) under osmotic stress and are hypersensitive to the oxidative stress reagent methyl viologen. The results suggest that SOS6/AtCSLD5 is not required for normal plant growth and development but has a critical role in osmotic stress tolerance and this function likely involves its regulation of ROS under stress.
authors.affiliation: Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USA. jhzhu@umd.edu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
authors.forename: Byeong-Ha
authors.initials: BH
authors.lastname: Lee
authors.forename: Mike
authors.initials: M
authors.lastname: Dellinger
authors.forename: Xinping
authors.initials: X
authors.lastname: Cui
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhang
authors.forename: Shang
authors.initials: S
authors.lastname: Wu
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Nothnagel
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhu
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D008353:Mannitol / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D009995:Osmosis; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D017382:Reactive Oxygen Species; D008353:Mannitol; D011092:Polyethylene Glycols; D012965:Sodium Chloride; C526694:CSLD5 protein, Arabidopsis; D005964:Glucosyltransferases; D010269:Paraquat
references.citation: Anal Biochem. 1973 Aug;54(2):484-9
references.pmid: 4269305
references.citation: Plant Physiol. 2003 Feb;131(2):547-57
references.pmid: 12586879
references.citation: Curr Biol. 2007 Jan 9;17(1):54-9
references.pmid: 17208187
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1999;50:391-417
references.pmid: 11541953
references.citation: J Biol Chem. 2000 Nov 24;275(47):36726-33
references.pmid: 10952990
references.citation: Plant Cell. 1996 Apr;8(4):617-627
references.pmid: 12239394
references.citation: EMBO J. 1987 Dec 20;6(13):3901-7
references.pmid: 3327686
references.citation: Plant Cell. 2001 Apr;13(4):873-87
references.pmid: 11283342
references.citation: Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3735-40
references.pmid: 10725350
references.citation: J Biol Chem. 2004 Oct 1;279(40):41758-66
references.pmid: 15292193
references.citation: Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):2221-6
references.pmid: 15647349
references.citation: Curr Opin Plant Biol. 2003 Oct;6(5):441-5
references.pmid: 12972044
references.citation: J Biol Chem. 2004 Jan 23;279(4):2922-6
references.pmid: 14583601
references.citation: Plant Cell. 2006 Jul;18(7):1736-49
references.pmid: 16751345
references.citation: Plant Cell. 2003 Jan;15(1):19-32
references.pmid: 12509519
references.citation: J Biol Chem. 1991 Aug 25;266(24):15956-65
references.pmid: 1874742
references.citation: J Biol Chem. 2004 Jan 2;279(1):207-15
references.pmid: 14570921
references.citation: Biostatistics. 2007 Jan;8(1):118-27
references.pmid: 16632515
references.citation: Plant Mol Biol. 2001 Sep;47(1-2):131-43
references.pmid: 11554468
references.citation: Annu Rev Cell Dev Biol. 2006;22:53-78
references.pmid: 16824006
references.citation: Adv Exp Med Biol. 1999;464:77-89
references.pmid: 10335387
references.citation: Genes Dev. 2003 Apr 15;17(8):1043-54
references.pmid: 12672693
references.citation: Plant Cell. 1997 Nov;9(11):1935-49
references.pmid: 9401119
references.citation: Nat Biotechnol. 2003 Jan;21(1):81-5
references.pmid: 12469134
references.citation: Plant Physiol. 2000 Oct;124(2):495-8
references.pmid: 11027699
references.citation: Plant Cell. 2000 Sep;12(9):1667-78
references.pmid: 11006339
references.citation: Plant Physiol. 2006 Sep;142(1):193-206
references.pmid: 16844838
references.citation: Plant Physiol. 1995 Apr;107(4):1129-38
references.pmid: 7770522
references.citation: Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3730-4
references.pmid: 10725382
references.citation: Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):9061-6
references.pmid: 12070350
references.citation: Mol Cell Biol. 2007 Jul;27(14):5214-24
references.pmid: 17485445
references.citation: Plant Physiol. 2001 Jul;126(3):1024-30
references.pmid: 11457953
references.citation: Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):8436-41
references.pmid: 12034882
references.citation: Trends Plant Sci. 2002 Sep;7(9):405-10
references.pmid: 12234732
references.citation: Plant J. 2007 Dec;52(5):791-802
references.pmid: 17892446
references.citation: Science. 2006 Mar 31;311(5769):1940-2
references.pmid: 16574868
references.citation: Plant J. 2004 Oct;40(2):260-75
references.pmid: 15447652
references.citation: Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6896-901
references.pmid: 10823923
references.citation: Plant Physiol. 2001 Jun;126(2):575-86
references.pmid: 11402188
references.citation: Science. 1998 Jun 19;280(5371):1943-5
references.pmid: 9632394
references.citation: Annu Rev Plant Biol. 2002;53:247-73
references.pmid: 12221975
references.citation: Plant Cell. 2002 Dec;14(12):3009-28
references.pmid: 12468724
references.citation: Plant Cell. 1998 Feb;10(2):255-66
references.pmid: 9490748
references.citation: Trends Plant Sci. 1999 Sep;4(9):361-6
references.pmid: 10462769
references.citation: Plant Cell. 2003 Oct;15(10):2273-84
references.pmid: 12972670
references.citation: Plant Cell. 2001 Jun;13(6):1383-400
references.pmid: 11402167
references.citation: Plant J. 1993 May;3(5):637-46
references.pmid: 24049876
references.citation: Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21
references.pmid: 11309499
references.citation: Genes Dev. 2001 Jan 1;15(1):79-89
references.pmid: 11156607
references.citation: EMBO J. 2004 Apr 7;23(7):1647-56
references.pmid: 15044947
references.citation: Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):31-6
references.pmid: 11134512
references.citation: Plant Physiol. 2006 Jun;141(2):336-40
references.pmid: 16760484
references.citation: J Biol Chem. 1999 Nov 19;274(47):33274-8
references.pmid: 10559202
references.citation: Plant J. 2005 Jul;43(2):273-83
references.pmid: 15998313
references.citation: Plant Physiol. 2004 Sep;136(1):2831-42
references.pmid: 15347788
references.citation: Mol Plant Microbe Interact. 2004 Jan;17(1):109-17
references.pmid: 14714874