title: Salicylate-induced translocation of prestin having mutation in the GTSRH sequence to the plasma membrane.
abstract: Prestin is a key molecule for mammalian hearing. The present study investigated changes in characteristics of prestin by culturing prestin-transfected cells with salicylate, an antagonist of prestin. As a result, the plasma membrane localization of prestin bearing a mutation in the GTSRH sequence, which normally accumulates in the cytoplasm, was recovered. Moreover, the nonlinear capacitance of the majority of the mutants, which is a signature of prestin activity, was also recovered. Thus, the present study discovered a new effect of salicylate on prestin, namely, the promotion of the plasma membrane expression of prestin mutants in an active state.
authors.affiliation: Department of Bioengineering and Robotics, Tohoku University, Sendai, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Kumano
authors.forename: Koji
authors.initials: K
authors.lastname: Iida
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishihara
authors.forename: Michio
authors.initials: M
authors.lastname: Murakoshi
authors.forename: Kouhei
authors.initials: K
authors.lastname: Tsumoto
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Ikeda
authors.forename: Izumi
authors.initials: I
authors.lastname: Kumagai
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Wada
mesh_terms: D000818:Animals; D001483:Base Sequence; D001669:Biochemical Phenomena; D002462:Cell Membrane / Q000235:genetics* / Q000378:metabolism*; D002465:Cell Movement / Q000235:genetics; D025802:Electric Capacitance; D006309:Hearing / Q000235:genetics; D017136:Ion Transport / Q000235:genetics; D008322:Mammals / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D021381:Protein Transport / Q000235:genetics; D020156:Salicylic Acid / Q000378:metabolism*
chemical_list: D020156:Salicylic Acid