Electrostatic interactions of fluorescent molecules with dielectric interfaces studied by total internal reflection fluorescence correlation spectroscopy.
Electrostatic interactions between dielectric surfaces and different fluorophores used in ultrasensitive fluorescence microscopy are investigated using objective-based Total Internal Reflection Fluorescence Correlation Spectroscopy (TIR-FCS). The interfacial dynamics of cationic rhodamine 123 and rhodamine 6G, anionic/dianionic fluorescein, zwitterionic rhodamine 110 and neutral ATTO 488 are monitored at various ionic strengths at physiological pH. As analyzed by means of the amplitude and time-evolution of the autocorrelation function, the fluorescent molecules experience electrostatic attraction or repulsion at the glass surface depending on their charges. Influences of the electrostatic interactions are also monitored through the triplet-state population and triplet relaxation time, including the amount of detected fluorescence or the count-rate-per-molecule parameter. These TIR-FCS results provide an increased understanding of how fluorophores are influenced by the microenvironment of a glass surface, and show a promising approach for characterizing electrostatic interactions at interfaces.
Blom
Hans
H
Department of Biomolecular Physics, Royal Institute of Technology, Stockholm, Sweden. hblom@kth.se <hblom@kth.se>
Hassler
Kai
K
Chmyrov
Andriy
A
Widengren
Jerker
J
D019793:Fluorescein / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D020112:Rhodamine 123 / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D013499:Surface Properties
C585059:Atto 488 maleimide; D005452:Fluoresceins; D005456:Fluorescent Dyes; D012235:Rhodamines; C026188:rhodamine 6G; C049025:rhodamine 110; D020112:Rhodamine 123; D019793:Fluorescein
Biol Chem. 2001 Mar;382(3):393-7
11347886
J Biomed Opt. 2001 Jan;6(1):6-13
11178575
Opt Express. 2005 Sep 19;13(19):7415-23
19498766
J Colloid Interface Sci. 2008 Jan 15;317(2):449-57
17950302
J Phys Chem B. 2005 May 12;109(18):8820-32
16852048
Talanta. 2002 Feb 11;56(2):365-73
18968508
Science. 1998 Sep 11;281(5383):1650-3
9733506
Biophys J. 2008 Dec 15;95(12):5789-97
18931261
J Phys Chem A. 2009 May 14;113(19):5554-66
19374408
Appl Opt. 2002 Nov 1;41(31):6614-20
12412652
J Phys Chem B. 2006 Jun 8;110(22):10910-8
16771344
Annu Rev Biophys Bioeng. 1984;13:247-68
6378070
Phys Rev E Stat Nonlin Soft Matter Phys. 2005 Oct;72(4 Pt 1):042101
16383445
Opt Express. 2009 Dec 21;17(26):23975-85
20052108
Phys Rev Lett. 2009 Jun 5;102(22):228101
19658903
Anal Chem. 2000 Jul 15;72(14):3260-5
10939397
Anal Chem. 1998 Jul 1;70(13):2565-75
21644776
J Mol Biol. 1994 Jul 22;240(4):372-84
8035460
Science. 1997 Feb 21;275(5303):1106-9
9027307
J Colloid Interface Sci. 2003 May 15;261(2):402-10
16256549
Anal Chem. 1996 Dec 1;68(23):4119-23
21619320
J Colloid Interface Sci. 2007 May 15;309(2):194-224
17368660
J Struct Biol. 2009 Oct;168(1):95-106
19269331
Cell Mol Life Sci. 2001 Sep;58(10):1451-60
11693526
Science. 1987 Jun 12;236(4807):1460-3
3589666
Biophys J. 2005 Jan;88(1):L01-3
15531630
J Biomed Opt. 2006 Jul-Aug;11(4):040502
16965125
Phys Rev Lett. 2008 Sep 12;101(11):114503
18851287
Biopolymers. 1974 Jan;13(1):29-61
4818131
Opt Express. 2007 Apr 30;15(9):5366-75
19532790