title: Design of targeted cardiovascular molecular imaging probes.
abstract: Molecular imaging relies on the development of sensitive and specific probes coupled with imaging hardware and software to provide information about the molecular status of a disease and its response to therapy, which are important aspects of disease management. As genomic and proteomic information from a variety of cardiovascular diseases becomes available, new cellular and molecular targets will provide an imaging readout of fundamental disease processes. A review of the development and application of several cardiovascular probes is presented here. Strategies for labeling cells with superparamagnetic iron oxide nanoparticles enable monitoring of the delivery of stem cell therapies. Small molecules and biologics (e.g., proteins and antibodies) with high affinity and specificity for cell surface receptors or cellular proteins as well as enzyme substrates or inhibitors may be labeled with single-photon-emitting or positron-emitting isotopes for nuclear molecular imaging applications. Labeling of bispecific antibodies with single-photon-emitting isotopes coupled with a pretargeting strategy may be used to enhance signal accumulation in small lesions. Emerging nanomaterials will provide platforms that have various sizes and structures and that may be used to develop multimeric, multimodal molecular imaging agents to probe one or more targets simultaneously. These platforms may be chemically manipulated to afford molecules with specific targeting and clearance properties. These examples of molecular imaging probes are characteristic of the multidisciplinary nature of the extraction of advanced biochemical information that will enhance diagnostic evaluation and drug development and predict clinical outcomes, fulfilling the promise of personalized medicine and improved patient care.
authors.affiliation: Mallinckrodt Institute of Radiology, School of Medicine, Washington University, St. Louis, Missouri, USA.
authors.forename: Carolyn J
authors.initials: CJ
authors.lastname: Anderson
authors.forename: Jeff W M
authors.initials: JW
authors.lastname: Bulte
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
authors.forename: Ban-An
authors.initials: BA
authors.lastname: Khaw
authors.forename: Monica
authors.initials: M
authors.lastname: Shokeen
authors.forename: Karen L
authors.initials: KL
authors.lastname: Wooley
authors.forename: Henry F
authors.initials: HF
authors.lastname: VanBrocklin
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism; D003952:Diagnostic Imaging / Q000379:methods*; D004452:Echocardiography / Q000379:methods; D004867:Equipment Design; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry; D015335:Molecular Probes / Q000494:pharmacology*; D036103:Nanotechnology / Q000379:methods; D049268:Positron-Emission Tomography / Q000379:methods; D011868:Radioisotopes / Q000494:pharmacology; D012984:Software; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods; D014057:Tomography, X-Ray Computed / Q000379:methods
chemical_list: D000906:Antibodies; D005290:Ferric Compounds; D015335:Molecular Probes; D011868:Radioisotopes; C000499:ferric oxide
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