title: Characterization of dental caries by LIF spectroscopy with 404-nm excitation.
abstract: The potential of laser-induced fluorescence (LIF) spectroscopy for the characterization of different stages of dental caries using 404-nm diode laser excitation was investigated. In vitro spectra from 16 sound, 10 noncavitated carious and 10 cavitated carious molar teeth were recorded on a miniature fibre-optic spectrometer. The areas under the receiver operating characteristics (ROC-AUC) were calculated and one-way analysis of variance (ANOVA) was performed. The LIF spectra of the carious teeth showed two peaks at 635 and 680 nm in addition to a broad band seen at 500 nm in sound teeth. The fluorescence intensity ratios, F500/F635 and F500/F680, in carious teeth were always lower than those in sound teeth. The ROC-AUC for discriminating between carious and sound teeth was 0.94, and for discriminating between noncavitated and cavitated carious teeth was 0.87. Statistically significant differences (p<0.001) were seen between sound, noncavitated carious and cavitated carious teeth. The results showed that LIF spectroscopy has the potential to be useful for characterizing different stages of caries in a clinical setting.
authors.affiliation: Biophotonics Laboratory, Centre for Earth Science Studies, Trivandrum, India.
authors.forename: Shiny Sara
authors.initials: SS
authors.lastname: Thomas
authors.forename: J L
authors.initials: JL
authors.lastname: Jayanthi
authors.forename: Narayanan
authors.initials: N
authors.lastname: Subhash
authors.forename: Joji
authors.initials: J
authors.lastname: Thomas
authors.forename: Rupananda J
authors.initials: RJ
authors.lastname: Mallia
authors.forename: G  N
authors.initials: G
authors.lastname: Aparna
mesh_terms: D003731:Dental Caries / Q000145:classification / Q000175:diagnosis*; D006801:Humans; D054023:Lasers, Semiconductor; D055100:Optical Fibers; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
references.citation: Lasers Surg Med. 2005 Oct;37(4):320-8
references.pmid: 16180220
references.citation: J Dent. 2005 Jan;33(1):73-8
references.pmid: 15652171
references.citation: Pediatr Dent. 2005 Jul-Aug;27(4):307-12
references.pmid: 16317971
references.citation: Adv Dent Res. 1997 Nov;11(4):507-14
references.pmid: 9470511
references.citation: Semin Nucl Med. 1978 Oct;8(4):283-98
references.pmid: 112681
references.citation: Lasers Med Sci. 2006 Apr;21(1):34-41
references.pmid: 16568211
references.citation: Lasers Surg Med. 2004;34(3):249-53
references.pmid: 15022252
references.citation: J Clin Pediatr Dent. 2006 Summer;30(4):287-91
references.pmid: 16937852
references.citation: Caries Res. 2001 Jan-Feb;35(1):21-6
references.pmid: 11125192
references.citation: Compend Contin Educ Dent. 2003;24(5 Suppl):3-11; quiz 19
references.pmid: 12830558
references.citation: Caries Res. 2005 Nov-Dec;39(6):490-5
references.pmid: 16251794
references.citation: Caries Res. 2000 Mar-Apr;34(2):151-8
references.pmid: 10773633
references.citation: Caries Res. 1998;32(3):210-8
references.pmid: 9577987
references.citation: Caries Res. 2001 Nov-Dec;35(6):464-70
references.pmid: 11799288
references.citation: J Dent Res. 1981 Feb;60(2):120-2
references.pmid: 6936452
references.citation: Cell Mol Biol (Noisy-le-grand). 1998 Dec;44(8):1293-300
references.pmid: 9874516
references.citation: Br Dent J. 2001 Apr 28;190(8):440-3
references.pmid: 11352392
references.citation: J Biomed Opt. 2007 Nov-Dec;12(6):064013
references.pmid: 18163829