title: 5-HT3 receptor-dependent modulation of respiratory burst frequency, regularity, and episodicity in isolated adult turtle brainstems.
abstract: To determine the role of central serotonin 5-HT(3) receptors in respiratory motor control, respiratory motor bursts were recorded from hypoglossal (XII) nerve rootlets on isolated adult turtle brainstems during bath-application of 5-HT(3) receptor agonists and antagonists. mCPBG and PBG (5-HT(3) receptor agonists) acutely increased XII burst frequency and regularity, and decreased bursts/episode. Tropisetron and MDL72222 (5-HT(3) antagonists) increased bursts/episode, suggesting endogenous 5-HT(3) receptor activation modulates burst timing in vitro. Tropisetron blocked all mCPBG effects, and the PBG-induced reduction in bursts/episode. Tropisetron application following mCPBG application did not reverse the long-lasting (2h) mCPBG-induced decrease in bursts/episode. We conclude that endogenous 5-HT(3) receptor activation regulates respiratory frequency, regularity, and episodicity in turtles and may induce a form of respiratory plasticity with the long-lasting changes in respiratory regularity.
authors.affiliation: Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Drive, Madison, WI 53706, USA.
authors.forename: Michelle E
authors.initials: ME
authors.lastname: Bartman
authors.forename: Julia E R
authors.initials: JE
authors.lastname: Wilkerson
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Johnson
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D001645:Biguanides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007002:Hypoglossal Nerve / Q000502:physiology; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D010812:Physical Stimulation / Q000379:methods; D044406:Receptors, Serotonin, 5-HT3 / Q000502:physiology*; D012119:Respiration / Q000187:drug effects; D012125:Respiratory Center / Q000166:cytology / Q000502:physiology*; D058827:Serotonin 5-HT3 Receptor Agonists; D058831:Serotonin 5-HT3 Receptor Antagonists; D018490:Serotonin Agents / Q000494:pharmacology; D013997:Time Factors; D014326:Tropanes / Q000494:pharmacology; D000077526:Tropisetron; D014426:Turtles / Q000033:anatomy & histology*
chemical_list: D001645:Biguanides; D007211:Indoles; D044406:Receptors, Serotonin, 5-HT3; D058827:Serotonin 5-HT3 Receptor Agonists; D058831:Serotonin 5-HT3 Receptor Antagonists; D018490:Serotonin Agents; D014326:Tropanes; D000077526:Tropisetron; C065734:1-(3-chlorophenyl)biguanide; C042253:bemesetron; C008846:phenyl biguanide
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