Granulocyte colony-stimulating factor facilitates recovery of retinal function following retinal ischemic injury.
The purpose of the present study was to investigate whether systemically administered granulocyte colony-stimulating factor (G-CSF) can protect against acute ischemic reperfusion injury. Two groups of anesthetized adult male Lewis rats (n = 8 per group) were subjected to an acute (45 min) episode of retinal ischemic injury followed by subcutaneous administration of vehicle (5% dextrose) or G-CSF (0.1 mg/kg/day) once per day x 5 days. Prior to and one week following ischemic insult, retinal function was measured by scotopic electroretinography (ERG). Retinas were harvested and morphologically analyzed one week after ischemic insult. ERG a- and b-wave amplitudes were significantly reduced following ischemic reperfusion injury. G-CSF treatment attenuated ischemic-induced loss of retinal function. In control vehicle-treated rats, ischemic reperfusion injury elicited marked and selective thinning of inner retinal layers while only minimally affecting outer retinal layers. Therapeutically administered G-CSF minimized ischemic-mediated thinning of whole retina and inner retinal layers. G-CSF may be of therapeutic interest for the management of retinal ischemic disorders.
Bu
Ping
P
Department of Ophthalmology, Loyola University Chicago, Maywood, IL 60153, USA.
Basith
Bilquis
B
Stubbs
Evan B
EB
Perlman
Jay I
JI
D000818:Animals; D004596:Electroretinography; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D007429:Intraocular Pressure; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D012160:Retina / Q000502:physiology*; D012164:Retinal Diseases / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D012165:Retinal Ganglion Cells / Q000378:metabolism; D012171:Retinal Vessels
D016188:Receptors, Granulocyte Colony-Stimulating Factor; D016179:Granulocyte Colony-Stimulating Factor