Variability in C(3)-plant cell-wall biosynthesis in a high-CO(2) atmosphere by solid-state NMR spectroscopy.
We have used a frequency-selective rotational-echo double-resonance (REDOR) solid-state NMR experiment to measure the concentrations of glycine-glycine pairs in proteins (and protein precursors) of intact leaves of plants exposed to both high- and low-CO(2) atomospheres. The results are interpreted in terms of differences in cell-wall biosynthesis between plant species. We illustrate this variability by comparing the assimilation of label in cheatgrass and soybean leaves labeled using (15)N-fertilizer and (13)CO(2) atmospheres. Cheatgrass and soybean are both C(3) plants but differ in their response to a high-CO(2) environment. Based on REDOR results, we determined that cheatgrass (a plant that seems likely to flourish in future low-water, high-CO(2) environments) routes 2% of the assimilated carbon label that remains in the leaf after 1 h in a 600-ppm (13)CO(2) atmosphere to glycine-rich protein (or its precursors), a structural component of cell walls cross-linked to lignins. In contrast, soybean under the same conditions routes none of its assimilated carbon to glycine-rich protein.
Yu
Tsyr-Yan
TY
Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA.
Singh
Manmilan
M
Matsuoka
Shigeru
S
Patti
Gary J
GJ
Potter
Gregory S
GS
Schaefer
Jacob
J
D031724:Bromus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D002245:Carbon Dioxide / Q000494:pharmacology*; D002473:Cell Wall / Q000187:drug effects* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D009682:Magnetic Resonance Spectroscopy; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013025:Glycine max / Q000187:drug effects / Q000378:metabolism
D002245:Carbon Dioxide; D002244:Carbon