In situ detection and genotyping of individual mRNA molecules.
Increasing knowledge about the heterogeneity of mRNA expression within cell populations highlights the need to study transcripts at the level of single cells. We present a method for detection and genotyping of individual transcripts based on padlock probes and in situ target-primed rolling-circle amplification. We detect a somatic point mutation, differentiate between members of a gene family and perform multiplex detection of transcripts in human and mouse cells and tissue.
Larsson
Chatarina
C
Department of Genetics and Pathology, Uppsala University, Sweden.
Grundberg
Ida
I
Söderberg
Ola
O
Nilsson
Mats
M
D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D017354:Point Mutation; D012333:RNA, Messenger / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis; D014158:Transcription, Genetic
D000199:Actins; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human
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