Juvenile hormone regulation of vitellogenin synthesis in the red flour beetle, Tribolium castaneum.
To elucidate the endocrine regulation of vitellogenin (Vg) synthesis in the red flour beetle, Tribolium castaneum, the titers of juvenile hormone (JH) and ecdysteroids in the whole body of female beetles were measured and compared with Vg mRNA levels. Juvenile hormone levels remained high while the ecdysteroid levels declined steadily during 1-5 days post adult emergence (PAE). The Vg mRNA levels began to increase by the end of 3rd day PAE and peaked by the 4th-5th day PAE. Gene expression profiling by microarray and quantitative real-time PCR analyses of RNA isolated from 1 to 5 days PAE beetles revealed that the genes coding for proteins involved in JH biosynthesis and action, but not those involved in 20-hydroxyecdysone (20E) biosynthesis and action had similar expression patterns as the genes coding for Vg. RNA interference (RNAi)-aided knock-down in the expression of these genes showed that both JH and 20E were required for Vg gene expression. However, Vg mRNA was induced by the application of JH III but not by the injection of 20E into the previtellogenic females. These data suggest that JH is required for Vg synthesis in the fat body and 20E influences Vg synthesis through its action on oocyte maturation.
Parthasarathy
R
R
Department of Entomology, College of Agriculture, University of Kentucky, Lexington, KY 40546, USA.
Sun
Zhiyuan
Z
Bai
Hua
H
Palli
Subba R
SR
D000818:Animals; D004441:Ecdysterone / Q000096:biosynthesis*; D005216:Fat Body / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D007605:Juvenile Hormones / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D013997:Time Factors; D014231:Tribolium / Q000378:metabolism*; D014819:Vitellogenins / Q000096:biosynthesis*
D007605:Juvenile Hormones; D012333:RNA, Messenger; D014819:Vitellogenins; D004441:Ecdysterone
Mech Dev. 2008 Mar-Apr;125(3-4):299-313
18083350
Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13773-8
14610274
J Insect Physiol. 1970 Jul;16(7):1455-66
5432388
Dev Biol. 1996 Feb 1;173(2):475-89
8606006
J Biol Chem. 2004 Aug 20;279(34):35942-9
15197185
Mech Dev. 2008 Jan-Feb;125(1-2):91-105
18036785
Insect Biochem Mol Biol. 2008 Jul;38(7):714-20
18549957
Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10488-93
17537916
Gen Comp Endocrinol. 2009 Jan 1;160(1):102-8
19032957
Recent Prog Horm Res. 1994;49:167-95
8146423
Annu Rev Entomol. 1992;37:217-51
1311540
J Biol Chem. 1993 Feb 15;268(5):3282-8
7679110
J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2006 Mar;192(3):315-20
16283330
Insect Biochem Mol Biol. 1999 Sep;29(9):821-7
10510500
Comp Biochem Physiol A Comp Physiol. 1985;80(1):115-21
2858283
Insect Biochem Mol Biol. 2004 Sep;34(9):991-1010
15350618
Insect Biochem Mol Biol. 2010 Jun;40(6):429-39
20385235
J Insect Physiol. 2008 Dec;54(12):1447-58
18789336
J Insect Physiol. 2000 Feb;46(2):153-160
12770247
FEBS J. 2008 Jun;275(11):2919-31
18435763
Mol Cell Endocrinol. 2008 Sep 10;291(1-2):42-9
18583027
Mech Dev. 2009 Jul;126(7):563-79
19324087
Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11350-5
15277665
DNA. 1987 Aug;6(4):331-42
2820677