title: Sclerosis therapy of bronchial artery attenuates acute lung injury induced by burn and smoke inhalation injury in ovine model.
abstract: BACKGROUND
  In burned sheep, we showed more than a 10-fold increase in bronchial blood flow following smoke inhalation. It was previously reported that sclerosis of the bronchial artery prior to smoke exposure reduces the pathophysiology of the inhalation insult. We hypothesized that sclerosis of the bronchial artery after insult attenuates smoke/burn-induced acute lung injury.
  
  
  METHODS
  Through an incision at the 4th intercostal space, a catheter was placed via the esophageal artery into the bronchial artery such that the bronchial blood flow remained intact. Acute lung injury was induced by a 40% total body surface area, 3rd degree cutaneous burn and smoke inhalation. Adult female sheep (n=18, 35.6±1.0 kg) were divided into three groups following the injury: (1) sclerosis group: 1h after injury, 4 mL of 70% ethanol was injected into bronchial artery via bronchial catheter, n=6; (2) control group: 1h after injury, an equal dose of saline was injected into bronchial artery via the bronchial catheter, n=6; (3) sham group: no injury and no treatment, n=6. The experiment was conducted in awake animals for 24 h.
  
  
  RESULTS
  Bronchial blood flow, measured by microspheres, was significantly reduced after ethanol injection in the sclerosis group. Pulmonary function, evaluated by measurement of blood gas analysis, pulmonary mechanics, and pulmonary transvascular fluid flux, was severely impaired in the control group. However, pulmonary function was significantly improved by bronchial artery sclerosis.
  
  
  CONCLUSIONS
  The results of our study clearly demonstrate a crucial role of enhanced bronchial circulation in thermal injury-related morbidity. Decreasing bronchial circulation using pharmacological agents may be an effective strategy in management of burn patients with concomitant smoke inhalation injury.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo 162-8666, Japan. a.hamahata@prs.twmu.ac.jp
authors.forename: Atsumori
authors.initials: A
authors.lastname: Hamahata
authors.forename: Perenlei
authors.initials: P
authors.lastname: Enkhbaatar
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakurai
authors.forename: Motohiro
authors.initials: M
authors.lastname: Nozaki
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Traber
mesh_terms: D055371:Acute Lung Injury / Q000503:physiopathology*; D000818:Animals; D001980:Bronchi / Q000098:blood supply*; D001981:Bronchial Arteries / Q000503:physiopathology; D004195:Disease Models, Animal; D000431:Ethanol / Q000627:therapeutic use*; D005260:Female; D011652:Pulmonary Circulation / Q000187:drug effects; D012039:Regional Blood Flow / Q000187:drug effects; D012597:Sclerosing Solutions / Q000627:therapeutic use*; D034561:Sheep, Domestic; D015208:Smoke Inhalation Injury / Q000503:physiopathology*
chemical_list: D012597:Sclerosing Solutions; D000431:Ethanol
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