Cyanines as new fluorescent probes for DNA detection and two-photon excited bioimaging.
A series of cyanine fluorophores based on fused aromatics as an electron donor for DNA sensing and two-photon bioimaging were synthesized, among which the carbazole-based biscyanine exhibits high sensitivity and efficiency as a fluorescent light-up probe for dsDNA, which shows selective binding toward the AT-rich regions. The synergetic effect of the bischromophoric skeleton gives a several-fold enhancement in a two-photon absorption cross-section as well as a 25- to 100-fold enhancement in two-photon excited fluorescence upon dsDNA binding.
Feng
Xin Jiang
XJ
Centre for Advanced Luminescence Materials, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China.
Wu
Po Lam
PL
Bolze
Frédéric
F
Leung
Heidi W C
HW
Li
King Fai
KF
Mak
Nai Ki
NK
Kwong
Daniel W J
DW
Nicoud
Jean-François
JF
Cheah
Kok Wai
KW
Wong
Man Shing
MS
D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009570:Nitriles / Q000138:chemical synthesis / Q000737:chemistry*; D017785:Photons
D005456:Fluorescent Dyes; D009570:Nitriles; D004247:DNA; C427203:calf thymus DNA