Characterization of H5N2 influenza viruses isolated in South Korea and their influence on the emergence of a novel H9N2 influenza virus.
We characterized low pathogenic avian influenza (LPAI) H5N2 and H9N2 viruses isolated in South Korea from 2008 to 2009. Genetic analysis of the H5N2 viruses isolated from wild birds and domestic ducks demonstrated that they were related to the recently isolated southern Chinese LPAI H5 viruses and various influenza viruses circulating in Eurasia. Three H9N2 viruses obtained at live bird markets and duck farms were reassortant viruses generated from the H5N2 viruses of domestic ducks and the H9N2 virus endemic in Korean chickens. The H5N2 viruses did not replicate well in experimentally infected chickens and mice, but novel H9N2 viruses, without pre-adaptation, were recovered at high titres in chickens. Our results show that reassortment between H5N2 and H9N2 viruses must have occurred in domestic ducks and may have contributed to the diversity expansion of the gene pool, which has potential to alter the pathogenicity and host range of the influenza virus.
Kim
Hye-Ryoung
HR
Animal Disease Diagnosis Center, National Veterinary Research and Quarantine Service, 335 Joongangro, Manangu, Anyangsi, Gyeonggido 430-824, Republic of Korea.
Park
Choi-Ku
CK
Animal Disease Diagnosis Center, National Veterinary Research and Quarantine Service, 335 Joongangro, Manangu, Anyangsi, Gyeonggido 430-824, Republic of Korea.
Oem
Jae-Ku
JK
Animal Disease Diagnosis Center, National Veterinary Research and Quarantine Service, 335 Joongangro, Manangu, Anyangsi, Gyeonggido 430-824, Republic of Korea.
Bae
You-Chan
YC
Animal Disease Diagnosis Center, National Veterinary Research and Quarantine Service, 335 Joongangro, Manangu, Anyangsi, Gyeonggido 430-824, Republic of Korea.
Choi
Jun-Gu
JG
Avian Disease Division, National Veterinary Research and Quarantine Service, 335 Joongangro, Manangu, Anyangsi, Gyeonggido 430-824, Republic of Korea.
Lee
O-Soo
OS
Animal Disease Diagnosis Center, National Veterinary Research and Quarantine Service, 335 Joongangro, Manangu, Anyangsi, Gyeonggido 430-824, Republic of Korea.
Lee
Youn-Jeong
YJ
Avian Disease Division, National Veterinary Research and Quarantine Service, 335 Joongangro, Manangu, Anyangsi, Gyeonggido 430-824, Republic of Korea.
D000818:Animals; D001717:Birds; D002645:Chickens; D016000:Cluster Analysis; D004372:Ducks; D053125:Influenza A Virus, H5N2 Subtype / Q000235:genetics* / Q000302:isolation & purification*; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics* / Q000302:isolation & purification; D005585:Influenza in Birds / Q000821:virology*; D051379:Mice; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000821:virology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics / Q000302:isolation & purification; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014779:Virus Replication
D012367:RNA, Viral