title: In vivo pink-beam imaging and fast alignment procedure for rat brain lesion microbeam radiation therapy.
abstract: A fast 50 microm-accuracy alignment procedure has been developed for the radiosurgery of brain lesions in rats, using microbeam radiation therapy. In vivo imaging was performed using the pink beam (35-60 keV) produced by the ID17 wiggler at the ESRF opened at 120 mm and filtered. A graphical user interface has been developed in order to define the irradiation field size and to position the target with respect to the skull structures observed in X-ray images. The method proposed here allows tremendous time saving by skipping the swap from white beam to monochromatic beam and vice versa. To validate the concept, the somatosensory cortex or thalamus of GAERS rats were irradiated under several ports using this alignment procedure. The magnetic resonance images acquired after contrast agent injection showed that the irradiations were selectively performed in these two expected brain regions. Image-guided microbeam irradiations have therefore been realised for the first time ever, and, thanks to this new development, the ID17 biomedical beamline provides a major tool allowing brain radiosurgery trials on animal patients.
authors.affiliation: European Synchrotron Radiation Facility, Grenoble, France. raph.serduc@gmail.com
authors.forename: Raphaël
authors.initials: R
authors.lastname: Serduc
authors.forename: Gilles
authors.initials: G
authors.lastname: Berruyer
authors.forename: Thierry
authors.initials: T
authors.lastname: Brochard
authors.forename: Michel
authors.initials: M
authors.lastname: Renier
authors.forename: Christian
authors.initials: C
authors.lastname: Nemoz
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000528:radiation effects*; D002138:Calibration; D008279:Magnetic Resonance Imaging; D016634:Radiosurgery; D051381:Rats; D014584:User-Computer Interface
references.citation: Eur Heart J. 2005 Jul;26(13):1284-91
references.pmid: 15734769
references.citation: PLoS Biol. 2008 Dec 23;6(12):2683-97
references.pmid: 19108604
references.citation: Med Phys. 2003 Apr;30(4):583-9
references.pmid: 12722810
references.citation: Rev Sci Instrum. 2009 Jul;80(7):074301
references.pmid: 19655968
references.citation: J Cereb Blood Flow Metab. 2003 Apr;23(4):499-512
references.pmid: 12679727
references.citation: Radiology. 2008 Oct;249(1):321-7
references.pmid: 18796684
references.citation: Phys Med Biol. 2009 Nov 7;54(21):6711-24
references.pmid: 19841517
references.citation: PLoS One. 2010 Feb 03;5(2):e9028
references.pmid: 20140254
references.citation: Int J Cancer. 1998 Nov 23;78(5):654-60
references.pmid: 9808538
references.citation: Int J Radiat Oncol Biol Phys. 2010 Dec 1;78(5):1503-12
references.pmid: 20932689
references.citation: J Synchrotron Radiat. 2006 May;13(Pt 3):260-70
references.pmid: 16645252
references.citation: J Neurosci. 2007 Jun 13;27(24):6590-9
references.pmid: 17567820
references.citation: J Neurol Neurosurg Psychiatry. 2000 May;68(5):563-70
references.pmid: 10766883
references.citation: Neurosurgery. 2001 Jan;48(1):70-6; discussion 76-7
references.pmid: 11152363
references.citation: Am J Respir Crit Care Med. 2009 Aug 15;180(4):296-303
references.pmid: 19483115
references.citation: Neurosurgery. 2009 Feb;64(2):289-96; discussion 296
references.pmid: 19057423
references.citation: Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8783-7
references.pmid: 7568017
references.citation: Int J Radiat Oncol Biol Phys. 2006 Apr 1;64(5):1519-27
references.pmid: 16580502
references.citation: Adv Drug Deliv Rev. 1999 Apr 5;36(2-3):145-163
references.pmid: 10837713
references.citation: J Am Chem Soc. 2008 May 7;130(18):5908-15
references.pmid: 18407638
references.citation: Phys Med Biol. 2008 Mar 7;53(5):1153-66
references.pmid: 18296755
references.citation: Med Phys. 1992 Nov-Dec;19(6):1395-400
references.pmid: 1461201
references.citation: J Synchrotron Radiat. 2009 Jul;16(Pt 4):587-90
references.pmid: 19535875
references.citation: Phys Med Biol. 2008 Jul 7;53(13):3609-22
references.pmid: 18560052
references.citation: Clin Oncol (R Coll Radiol). 1999;11(5):303-20
references.pmid: 10591819
references.citation: Lancet Neurol. 2006 Jul;5(7):613-20
references.pmid: 16781991
references.citation: Eur Radiol. 2000;10(9):1487-92
references.pmid: 10997441