title: Dammarane-type saponins from Gynostemma pentaphyllum.
abstract: Dammarane-type saponins, gypenosides VN1-VN7 (1-7), were isolated from the total saponin extract of Gynostemma pentaphyllum aerial parts, with their structures elucidated on the basis of spectroscopic and chemical methods. These compounds showed moderate cytotoxic activity against four human cancer cell lines, A549 (lung), HT-29 (colon), MCF-7 (breast), and SK-OV-3 (ovary), with IC(50) values ranging from 19.6+/-1.1 to 43.1+/-1.0 microM. Regarding the HL-60 (acute promyelocytic leukemia) cell line, compounds 1, 5, and 6 showed weakly active with IC(50) values of 62.8+/-1.9, 72.6+/-3.6, and 82.4+/-3.2 nM, respectively, while 2, 3, 4, and 7 were less active with IC(50) values>100 microM.
authors.affiliation: Hanoi University of Pharmacy, 17 Le Thanh Tong, Hanoi, Vietnam.
authors.forename: Pham Thanh
authors.initials: PT
authors.lastname: Ky
authors.forename: Pham Thanh
authors.initials: PT
authors.lastname: Huong
authors.forename: Than Kieu
authors.initials: TK
authors.lastname: My
authors.forename: Pham Tuan
authors.initials: PT
authors.lastname: Anh
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Cuong
authors.forename: Nguyen Phuong
authors.initials: NP
authors.lastname: Thao
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Nhiem
authors.forename: Jae-Hee
authors.initials: JH
authors.lastname: Hyun
authors.forename: Hee-Kyoung
authors.initials: HK
authors.lastname: Kang
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005260:Female; D029774:Gynostemma / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014744:Vietnam; D000097669:Dammaranes
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; D014315:Triterpenes; D000097669:Dammaranes