Selenium-induced protection of photosynthesis activity in rape (Brassica napus) seedlings subjected to cadmium stress. Fluorescence and EPR measurements.
Fluorescence and electron paramagnetic resonance measurements were used to study selenium influence on photosystem activity in rape seedlings affected by Cd stress. Water cultures containing Hoagland nutrients were supplemented with 400 microM of CdCl(2), 2 microM of Na(2)SeO(4) and a mixture of both CdCl(2) and Na(2)SeO(4). The seedlings were cultured till the first leaf reached about 1 cm in length. Cadmium-induced changes in the activity of both photosystems were partly diminished by Se presence in the nutrient medium. Electron microscopy photographs confirmed less degradation in chloroplasts of plants cultured on media containing Se. It is suggested that sucrose groups of starch, which is deposited in greater amounts in Cd-stressed plants, may act as traps for free radicals produced under those conditions.
Filek
Maria
M
Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland. mariafilek@excite.com
Kościelniak
Janusz
J
Łabanowska
Maria
M
Bednarska
Elzbieta
E
Bidzińska
Ewa
E
D029688:Brassica napus / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D002104:Cadmium / Q000633:toxicity*; D004578:Electron Spin Resonance Spectroscopy; D005453:Fluorescence; D046529:Microscopy, Electron, Transmission; D010788:Photosynthesis / Q000187:drug effects*; D036226:Seedlings / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D012643:Selenium / Q000494:pharmacology*
D002104:Cadmium; D012643:Selenium
Chem Pharm Bull (Tokyo). 2004 Mar;52(3):339-44
14993758
Biochemistry. 1997 Jul 15;36(28):8548-58
9214300
J Plant Physiol. 2008 May 26;165(8):833-44
17913288
Eur Biophys J. 1999;28(6):468-77
10460340
Proc Natl Acad Sci U S A. 1971 Mar;68(3):625-8
4993385
Proc Natl Acad Sci U S A. 2005 Nov 8;102(45):16215-20
16254054
Biochemistry. 1986 Apr 22;25(8):2276-87
3011083
Appl Radiat Isot. 2000 May;52(5):1221-7
10836434
Biol Trace Elem Res. 2003 Jun;92(3):257-74
12794277
J Radiat Res. 1999 Dec;40(4):303-10
10748576
Spectrochim Acta A Mol Biomol Spectrosc. 2006 Mar 13;63(4):795-801
16488658
Biochemistry. 1994 Aug 23;33(33):9922-8
8061000
Appl Radiat Isot. 2000 May;52(5):1229-34
10836435
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9485-8
8415727
FEBS Lett. 2003 Nov 27;555(1):45-50
14630317