Investigating the impact of cholesterol on magnetically aligned sphingomyelin/cholesterol multilamellar vesicles using static (31)P NMR.
The effect of cholesterol (5-40mol%) on the magnetic induced orientation of sphingomyelin/cholesterol multilamellar vesicles (MLVs) was examined using static solid state (31)P NMR spectroscopy. The orientation was modeled assuming an ellipsoidal deformation of the vesicles and was monitored as a function of cholesterol concentration and temperature. In addition, the static (31)P chemical shift anisotropy (CSA) was used to assess the motional and dynamical changes occurring in the bilayer are reported. An exploration of the factors determining the magnetic orientation in sphingomyelin/cholesterol bilayers from the gel (s(o)) to liquid crystalline (or liquid-ordered, l(o)) phases is presented and discussed.
Costello
Alison L
AL
Department of Electronic and Nanostructured Materials, Sandia National Laboratories, Albuquerque, NM 87185-0886, United States.
Alam
Todd M
TM
D002784:Cholesterol / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics; D010759:Phosphorus Isotopes / Q000737:chemistry; D013109:Sphingomyelins / Q000737:chemistry*; D013696:Temperature
D008051:Lipid Bilayers; D010759:Phosphorus Isotopes; D013109:Sphingomyelins; D002784:Cholesterol