[Changes of mitochondria membrane potential of the uterine smooth muscle under Mg2+ and Ca2+ influence].
Using the method of flow cytometry and potential-sensitive fluorescent dye TMRM the effect of divalent cations on the membrane potential of isolated myometrium mitochondria was studied. It was shown, that Mg2+ (7 mM) addition to the incubation medium induced mitochondrial membrane hyperpolarization. In the case of protonophore CCCP (1 microM) preliminary presence in the incubation medium, Mg2+ addition did not lead to membrane potential hyperpolarization. Addition of Ca24+ leads to membrane potential dissipation. Ca2+ -induced mitochondrial depolarization is the same as NaN3 (10 mM)-induced depolarization. It also was shown that Mg2+ (7 mM) preliminary presence in the incubation medium did not protect mitochondria from Ca2+ -induced depolarization.
Naumova
N V
NV
Babich
L H
LH
Shlykov
S H
SH
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D005260:Female; D015636:Magnesium Chloride / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008931:Mitochondria, Muscle / Q000187:drug effects*; D009215:Myometrium / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D019810:Sodium Azide / Q000494:pharmacology
D015636:Magnesium Chloride; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D019810:Sodium Azide; D002122:Calcium Chloride