Multisite study of cryptosporidiosis in children with diarrhea in India.
Cryptosporidium spp., a common cause of diarrhea in children, were investigated in the first multisite study in India. Diarrheal stools from hospitalized children aged <5 years from Delhi, Trichy, and Vellore were analyzed by microscopy, PCR-restriction fragment length polymorphism (RFLP), and/or sequencing at the small-subunit (SSU) rRNA and Cpgp40/15 loci for species determination and subgenotyping, respectively. Seventy of 2,579 (2.7%) children, 75% of whom were <2 years old, had cryptosporidial diarrhea as determined by microscopy. Genotyping and subgenotyping showed that Cryptosporidium hominis was the most commonly identified species (59/67 children), and subgenotypes Ie, Ia, Ib, and Id were common in all centers. A novel C. parvum subgenotype, IIn, was identified in Vellore. Meteorological analysis revealed a higher rate of cryptosporidial positivity during hotter and drier weather in Delhi.
Ajjampur
Sitara Swarna Rao
SS
Department of Gastrointestinal Sciences, Christian Medical College, Vellore 632004, India.
Liakath
Farzana Begum
FB
Kannan
Arun
A
Rajendran
Priya
P
Sarkar
Rajiv
R
Moses
Prabhakar Devarajan
PD
Simon
Anna
A
Agarwal
Indira
I
Mathew
Ann
A
O'Connor
Roberta
R
Ward
Honorine
H
Kang
Gagandeep
G
D002675:Child, Preschool; D002980:Climate; D016000:Cluster Analysis; D003457:Cryptosporidiosis / Q000453:epidemiology* / Q000469:parasitology; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology* / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D005260:Female; D020459:Genes, rRNA; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D008853:Microscopy; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S
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