title: Identification of Rotavirus VP6-Specific CD4+ T Cell Epitopes in a G1P[8] Human Rotavirus-Infected Rhesus Macaque.
abstract: A non-human primate model was used to evaluate its potential for identification of rotavirus viral protein 6 (VP6) CD4+ T cell epitopes. Four juvenile rhesus macaques were inoculated with a mixed inoculum (G1P[8] and G9P[8]) of human rotaviruses. Infection accompanied by G1P[8] shedding was achieved in the two macaques that had no rotavirus immunoglobulin A (IgA) in plasma. To measure the interferon gamma (IFN-γ) and tumor necrosis factor (TNF) anti-viral cytokines produced by peripheral CD4+ cells that recognize VP6 epitopes, whole blood cells from one infected macaque were stimulated in vitro with VP6 peptides. Stimulation with peptide pools derived from the simian rotavirus VP6(161-395) region revealed reactivity of CD4+ T cells with the VP6(281-331) domain. A VP6(301-315) region was identified as the epitope responsible for IFN-γ production while a broader VP6(293-327) domain was linked to TNF production. These results suggest that human rotavirus-infected macaques can be used for identification of additional epitopes and domains to address specific questions related to the development of pediatric vaccines.
authors.affiliation: Tulane National Primate Research Center, Covington, LA, U.S.A.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Bapi
authors.initials: B
authors.lastname: Pahar
authors.forename: Karol
authors.initials: K
authors.lastname: Sestak
references.citation: Arch Virol. 1976;50(1-2):17-27
references.pmid: 816334
references.citation: J Virol. 1997 Nov;71(11):8707-17
references.pmid: 9343229
references.citation: Am J Vet Res. 1980 Jul;41(7):1098-1103
references.pmid: 6254409
references.citation: J Clin Microbiol. 1992 Jun;30(6):1365-73
references.pmid: 1320625
references.citation: J Clin Microbiol. 1990 Feb;28(2):276-82
references.pmid: 2155916
references.citation: Vaccine. 2006 Nov 17;24(47-48):6839-49
references.pmid: 17050045
references.citation: J Med Virol. 2005 Apr;75(4):616-25
references.pmid: 15714478
references.citation: Vet Immunol Immunopathol. 2002 Sep 10;87(3-4):147-60
references.pmid: 12072229
references.citation: Emerg Infect Dis. 2003 May;9(5):565-72
references.pmid: 12737740
references.citation: J Virol. 2000 Dec;74(24):11574-80
references.pmid: 11090155
references.citation: J Virol. 2002 May;76(10):4741-9
references.pmid: 11967291
references.citation: J Virol. 2004 Oct;78(19):10258-64
references.pmid: 15367591
references.citation: Immunol Rev. 2001 Oct;183:76-85
references.pmid: 11782248
references.citation: Virol J. 2006 May 31;3:40
references.pmid: 16737519
references.citation: J Virol. 2006 May;80(10):4949-61
references.pmid: 16641286
references.citation: J Virol. 1998 Apr;72(4):2708-14
references.pmid: 9525588
references.citation: Virology. 2003 Jan 5;305(1):93-105
references.pmid: 12504544
references.citation: Virology. 2007 Jul 5;363(2):410-8
references.pmid: 17337285
references.citation: J Virol. 1991 May;65(5):2562-71
references.pmid: 1850029
references.citation: J Clin Microbiol. 1994 Jul;32(7):1820-2
references.pmid: 7929782
references.citation: J Virol. 2004 Oct;78(20):10967-76
references.pmid: 15452217
references.citation: J Virol. 2002 Jan;76(2):560-8
references.pmid: 11752147
references.citation: J Virol. 1998 Jan;72(1):330-8
references.pmid: 9420231
references.citation: Clin Infect Dis. 2002 May 15;34(10):1351-61
references.pmid: 11981731
references.citation: Comp Immunol Microbiol Infect Dis. 2008 Mar;31(2-3):253-69
references.pmid: 17698192
references.citation: Virology. 2005 Oct 25;341(2):248-56
references.pmid: 16095646
references.citation: J Virol. 1995 Dec;69(12):7800-6
references.pmid: 7494291
references.citation: Immunogenetics. 1997;45(3):171-9
references.pmid: 8995183
references.citation: Gut. 1977 Feb;18(2):156-60
references.pmid: 192637
references.citation: Virology. 2002 Mar 15;294(2):256-69
references.pmid: 12009867
references.citation: Virology. 2003 Sep 30;314(2):671-9
references.pmid: 14554094
references.citation: Infection. 1978;6(2):71-2
references.pmid: 206515
references.citation: J Virol. 2005 Jan;79(2):944-54
references.pmid: 15613323
references.citation: J Virol. 2005 Apr;79(8):4568-79
references.pmid: 15795243
references.citation: J Immunol Methods. 2001 Sep 1;255(1-2):27-40
references.pmid: 11470284
references.citation: Tissue Antigens. 2000 Jul;56(1):58-68
references.pmid: 10958357
references.citation: J Virol. 1997 Jan;71(1):419-26
references.pmid: 8985366