Accurate and simple sizing of primer extension products using a non-radioactive approach facilitates identification of transcription initiation sites.
An improved method of non-radioactive identification of transcription start sites is presented in which the use of 7-deaza dGTP in the primer extension reaction allows the product to be directly aligned to cycle sequencing traces on an automated sequencer. This removes the documented need to apply corrections for mobility differences.
Broadbent
Sarah
S
Department of Biology and the Hull York Medical School, University of York, United Kingdom.
van der Woude
Marjan
M
Aziz
Naveed
N
D001331:Automation / Q000379:methods; D020029:Base Pairing; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods; D024363:Transcription Initiation Site
C045550:7-deaza-2'-deoxyguanosine; D003849:Deoxyguanosine