Isolation and characterization of differentially expressed transcripts from the suspension cells of oil palm (Elaeis guineensis Jacq.) in response to different concentration of auxins.
Oil palm suspension cultures were initiated by transferring the gel-like friable embryogenic tissue onto liquid medium supplemented with auxins. In this study, transcripts that were differentially expressed in oil palm suspension cells cultured at different auxin concentrations were examined using suppression subtractive hybridization. Total RNA was first isolated from oil palm suspension cells proliferated in liquid medium with different hormone concentrations for 6 months. Four different hormone combinations: T1 (0.1 mg/l 2,4-D and 1.0 mg/l NAA), T2 (0.4 mg/l 2,4-D and 1.0 mg/l NAA), T3 (1.0 mg/l NAA), and T4 (0.4 mg/l 2,4-D) were used for the treatments. The first and second subtractions were performed using samples T1 and T2 in forward and reverse order. The other two subtractions were forward and reverse subtractions of T3 and T4, respectively. Reverse northern analyses showed that 14.13% of these clones were preferentially expressed in T1, 13.70% in T2, 14.75% in T3, and 15.70% in T4. Among the 294 cDNA clones that were sequenced, 61 contigs (assembled from 165 sequences) and 129 singletons were obtained. Among the 61 contigs, 10 contigs consist of sequences from treatment T1, 8 contigs were from treatment T2, 10 contigs were contains sequences of treatment T3 and 13 contigs contains sequences of treatment T4. Northern analyses of five transcripts that were shown to be differentially expressed in the oil palm suspension cells by reverse northern analysis revealed that transcripts 16A1 (a putative lignostilbene-alpha,beta-dioxygenase, EgLSD) and 16H12 (a putative ethylene responsive 6, EgER6) were differentially expressed in oil palm suspension cells treated with different levels of auxin.
Roowi
Siti Habsah
SH
Felda Biotechnology Centre, Lengkuk Technology, Bandar Enstek, Negeri Sembilan, Malaysia. shabsah.r@felda.net.my
Ho
Chai-Ling
CL
Alwee
Sharifah Shahrul Rabiah Syed
SS
Abdullah
Meilina Ong
MO
Napis
Suhaimi
S
D000595:Amino Acid Sequence; D028023:Arecaceae / Q000166:cytology* / Q000187:drug effects / Q000196:embryology / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D020451:Contig Mapping; D018076:DNA, Complementary / Q000235:genetics; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D015723:Gene Library; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D000073878:Palm Oil; D010938:Plant Oils / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013535:Suspensions
D018076:DNA, Complementary; D007210:Indoleacetic Acids; D010938:Plant Oils; D010940:Plant Proteins; D012333:RNA, Messenger; D013535:Suspensions; D000073878:Palm Oil
Plant Cell Physiol. 2001 Aug;42(8):879-83
11522916
Plant Cell Physiol. 2004 Apr;45(4):407-15
15111715
New Phytol. 2001 Jul;151(1):35-66
33873389
Plant Physiol. 1999 May;120(1):1-6
10318677
Plant Cell. 2002;14 Suppl:S15-45
12045268
Plant Cell Physiol. 1998 Nov;39(11):1184-93
9891417
Trends Plant Sci. 1999 Jul;4(7):269-274
10407443
FEBS Lett. 1999 Apr 16;449(1):57-60
10225428
J Biotechnol. 2004 Mar 18;108(3):253-63
15006426
J Exp Bot. 2002 Jul;53(374):1575-80
12096096
Fungal Genet Biol. 2002 Nov;37(2):197-204
12409104
Plant J. 1999 Jun;18(6):589-600
10417710
Science. 1997 Jun 20;276(5320):1872-4
9188535
Plant Physiol. 2005 Apr;137(4):1250-60
15749990
Plant Physiol. 2003 Jul;132(3):1664-77
12857845
Ann Bot. 2005 May;95(6):901-15
15753119
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
9254694
Plant Cell. 1995 Feb;7(2):173-82
7756828
Genome Res. 1999 Sep;9(9):868-77
10508846
Plant J. 2001 Sep;27(5):373-82
11576422