title: Identification of a new adhesin-like protein from Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens.
abstract: OBJECTIVE
  To identify and characterize a new adhesin-like protein of probiotics that show specific adhesion to human blood group A and B antigens.
  
  
  RESULTS
  Using the BIACORE assay, the adhesion of cell surface components obtained from four lactobacilli strains that adhered to blood group A and B antigens was tested. Their components showed a significant adhesion to A and B antigens when compared to the bovine serum albumin (BSA) control. The 1 mol l(-1) GHCl fraction extracted from Lactobacillus mucosae ME-340 contained a 29-kDa band (Lam29) using SDS-PAGE. The N-terminal amino acid sequence and homology analysis showed that Lam29 was 90% similar to the substrate-binding protein of the ATP-binding cassette (ABC) transporter from Lactobacillus fermentum IFO 3956. The complete nucleotide sequence (858 bp) of Lam29 was determined and encoded a protein of 285 amino acid residues. Phylogenetic analysis and multiple sequence alignments indicated this protein may be related to the cysteine-binding transporter.
  
  
  CONCLUSIONS
  The adhesion of ME-340 strain to blood group A and B antigens was mediated by Lam29 that is a putative component of ABC transporter as an adhesin-like protein.
  
  
  CONCLUSIONS
  Lactobacillus mucosae ME-340 expressing Lam29 may be useful for competitive exclusion of pathogens via blood group antigen receptors in the human gastrointestinal mucosa and in the development of new probiotic foods.
authors.affiliation: Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Kinoshita
authors.forename: M
authors.initials: M
authors.lastname: Nitta
authors.forename: R
authors.initials: R
authors.lastname: Yukishita
authors.forename: Y
authors.initials: Y
authors.lastname: Kawai
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: N
authors.initials: N
authors.lastname: Taketomo
authors.forename: Y
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Tateno
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: A
authors.initials: A
authors.lastname: Horii
authors.forename: H
authors.initials: H
authors.lastname: Kitazawa
authors.forename: T
authors.initials: T
authors.lastname: Saito
mesh_terms: D000017:ABO Blood-Group System / Q000378:metabolism*; D018829:Adhesins, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007778:Lactobacillus / Q000378:metabolism*; D008969:Molecular Sequence Data; D019936:Probiotics; D016415:Sequence Alignment; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D000017:ABO Blood-Group System; D018829:Adhesins, Bacterial; D012710:Serum Albumin, Bovine