title: [Microbial diversity and characteristics of cultivable microorganisms in bioleaching reactors].
abstract: OBJECTIVE
  In order to study the microbial diversity and the characteristics of cultivable microorganisms in bioleaching reactors.
  
  
  METHODS
  Both conventional culture technique and analysis of a 16S rRNA sequence library were used. The pure cultures were isolated by enriching the samples under different cultural conditions. The morphology characters, physiology and biochemistry characters, genetic characters and oxidation efficiency to different minerals of cultured isolates were analyzed.
  
  
  RESULTS
  The microbial community in bioleaching reactors identified through the gene clone libraries were closely related to bacteria including Leptospirillum sp., Sulfobacillus sp., Acidithiobacillus sp., Spingomonas sp. and Archaea including Sulfolobus sp., Ferroplasma sp. Five strains were isolated after enriching and culturing, which were corresponding to Acidithiooxidians caldus, Acidithiooxidians thiooxidans, Acidithiooxidans ferrooxidans, Leptospirillum ferriphilum and Sulfobacillus thermosulfidooxidans. These isolates could oxidize sulfur or Fe2+ and different sulfide ores.
  
  
  CONCLUSIONS
  The bioleaching reactors were dominated by a small number of microbial species that were commonly found at thermoacidophilic environments.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. liuyy515@hotmail.com
authors.forename: Yanyang
authors.initials: Y
authors.lastname: Liu
authors.forename: Xu
authors.initials: X
authors.lastname: Guo
authors.forename: Chengying
authors.initials: C
authors.lastname: Jiang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013440:Sulfides / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013440:Sulfides