title: Description of Holtermanniella gen. nov., including Holtermanniella takashimae sp. nov. and four new combinations, and proposal of the order Holtermanniales to accommodate tremellomycetous yeasts of the Holtermannia clade.
abstract: The novel genus Holtermanniella is proposed here to accommodate four Cryptococcus species closely related to Holtermannia corniformis that are included in the Holtermannia clade (Basidiomycota, Agaricomycotina). Thus, four novel combinations are proposed: Holtermanniella nyarrowii comb. nov., Holtermanniella festucosa comb. nov., Holtermanniella mycelialis comb. nov. and Holtermanniella wattica comb. nov. In addition, a novel anamorphic yeast species was studied with 15 isolates obtained from different habitats around the world. Analysis of the sequences of the D1/D2 region of their large subunit rDNA showed that the novel species is placed phylogenetically within the Holtermannia clade of the Tremellomycetes (Agaricomycotina, Basidiomycota). PCR fingerprinting and sequencing of ITS1-5.8S-ITS2 showed genetic intraspecific variability among the strains: three groups were formed, which did not correlate with geographical origin or substrate. This novel species, designated the type species of Holtermanniella gen. nov., is described as Holtermanniella takashimae sp. nov.; the type strain is CBS 11174(T) (=HB 982(T) =DBVPG 8012(T)). The order Holtermanniales ord. nov. is proposed here to include Holtermannia (the type genus) and Holtermanniella.
authors.affiliation: University of Natural Resources and Applied Life Sciences, Institute of Applied Microbiology (IAM), Austrian Center of Biological Resources and Applied Mycology (ACBR), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Wuczkowski
authors.affiliation: Swedish University of Agricultural Sciences (SLU), Department for Microbiology, Uppsala, Sweden.
authors.forename: Volkmar
authors.initials: V
authors.lastname: Passoth
authors.affiliation: Department of Applied Biology & Industrial Yeasts Collection DBVPG, Università di Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy.
authors.forename: Benedetta
authors.initials: B
authors.lastname: Turchetti
authors.affiliation: Swedish University of Agricultural Sciences (SLU), Department for Microbiology, Uppsala, Sweden.
authors.forename: Ann-Christin
authors.initials: AC
authors.lastname: Andersson
authors.affiliation: Swedish University of Agricultural Sciences (SLU), Department for Microbiology, Uppsala, Sweden.
authors.forename: Matilda
authors.initials: M
authors.lastname: Olstorpe
authors.affiliation: VTT Technical Research Centre of Finland, PO Box 1000, Tietotie 2, Espoo, 02044 VTT, Finland.
authors.forename: Arja
authors.initials: A
authors.lastname: Laitila
authors.affiliation: CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Bart
authors.initials: B
authors.lastname: Theelen
authors.affiliation: Lab. Microbiología Aplicada y Biotecnología, INIBIOMA, CONICET-UNComahue, Quintral 1250, (8400), Bariloche, Río Negro, Argentina.
authors.forename: María
authors.initials: M
authors.lastname: van Broock
authors.affiliation: Department of Applied Biology & Industrial Yeasts Collection DBVPG, Università di Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Buzzini
authors.affiliation: University of Natural Resources and Applied Life Sciences, Institute of Applied Microbiology (IAM), Austrian Center of Biological Resources and Applied Mycology (ACBR), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Hansjörg
authors.initials: H
authors.lastname: Prillinger
authors.affiliation: University of Natural Resources and Applied Life Sciences, Institute of Applied Microbiology (IAM), Austrian Center of Biological Resources and Applied Mycology (ACBR), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Katja
authors.initials: K
authors.lastname: Sterflinger
authors.affiliation: Swedish University of Agricultural Sciences (SLU), Department for Microbiology, Uppsala, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Schnürer
authors.affiliation: CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Teun
authors.initials: T
authors.lastname: Boekhout
authors.affiliation: Lab. Microbiología Aplicada y Biotecnología, INIBIOMA, CONICET-UNComahue, Quintral 1250, (8400), Bariloche, Río Negro, Argentina.
authors.forename: Diego
authors.initials: D
authors.lastname: Libkind
mesh_terms: D001487:Basidiomycota / Q000145:classification* / Q000235:genetics; D016000:Cluster Analysis; D003454:Cryptococcus / Q000145:classification* / Q000235:genetics; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S; C065096:RNA, ribosomal, 26S