title: Analysis of arsenical metabolites in biological samples.
abstract: Quantitation of iAs and its methylated metabolites in biological samples provides dosimetric information needed to understand dose-response relations. Here, methods are described for separation of inorganic and mono-, di-, and trimethylated arsenicals by thin layer chromatography. This method has been extensively used to track the metabolism of the radionuclide [(73)As] in a variety of in vitro assay systems. In addition, a hydride generation-cryotrapping-gas chromatography-atomic absorption spectrometric method is described for the quantitation of arsenicals in biological samples. This method uses pH-selective hydride generation to differentiate among arsenicals containing trivalent or pentavalent arsenic.
authors.affiliation: Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7310, USA.
authors.forename: Araceli
authors.initials: A
authors.lastname: Hernandez-Zavala
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Drobna
authors.forename: Miroslav
authors.initials: M
authors.lastname: Styblo
authors.forename: David J
authors.initials: DJ
authors.lastname: Thomas
mesh_terms: D001151:Arsenic / Q000378:metabolism*; D001152:Arsenicals / Q000032:analysis*; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D010084:Oxidation-Reduction; D011868:Radioisotopes / Q000378:metabolism; D013048:Specimen Handling; D013054:Spectrophotometry, Atomic / Q000295:instrumentation / Q000379:methods
chemical_list: D001152:Arsenicals; D011868:Radioisotopes; D001151:Arsenic
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