title: Metagenomic analysis of bronchoalveolar lavage samples from patients with idiopathic interstitial pneumonia and its antagonic relation with Pneumocystis jirovecii colonization.
abstract: Idiopathic interstitial pneumonias are interstitial lung diseases of unknown etiology which prognosis is usually fatal. Microbiota associated to bronchoalveolar lavage from 20 patients with negative bacterial cultures was explored by 16S-rDNA PCR-DGGE, showing a clearly negative relation among the presence of P. jirovecii and bacterial colonization. This is the first report of in vivo antagonistic relation among fungi and bacteria.
authors.affiliation: Instituto de Biomedicina de Sevilla, Hospital Universitario, Virgen del Rocío, Seville, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Friaza
authors.forename: Carmen de
authors.initials: Cd
authors.lastname: la Horra
authors.forename: Mario J
authors.initials: MJ
authors.lastname: Rodríguez-Domínguez
authors.forename: José
authors.initials: J
authors.lastname: Martín-Juan
authors.forename: Rafael
authors.initials: R
authors.lastname: Cantón
authors.forename: Enrique J
authors.initials: EJ
authors.lastname: Calderón
authors.forename: Rosa
authors.initials: R
authors.lastname: Del Campo
mesh_terms: D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D005260:Female; D006801:Humans; D054988:Idiopathic Interstitial Pneumonias / Q000382:microbiology*; D008297:Male; D056186:Metagenomics; D008875:Middle Aged; D008969:Molecular Sequence Data; D045363:Pneumocystis carinii / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*