title: Genome-wide profiling of humoral immune response to Coxiella burnetii infection by protein microarray.
abstract: Comprehensive evaluation of the humoral immune response to Coxiella burnetii may identify highly needed diagnostic antigens and potential subunit vaccine candidates. Here we report the construction of a protein microarray containing 1901 C. burnetii ORFs (84% of the entire proteome). This array was probed with Q-fever patient sera and naïve controls in order to discover C. burnetii-specific seroreactive antigens. Among the 21 seroreactive antigens identified, 13 were significantly more reactive in Q-fever cases than naïve controls. The remaining eight antigens were cross-reactive in both C. burnetii infected and naïve patient sera. An additional 64 antigens displayed variable seroreactivity in Q-fever patients, and underscore the diversity of the humoral immune response to C. burnetii. Nine of the differentially reactive antigens were validated on an alternative immunostrip platform, demonstrating proof-of-concept development of a consistent, safe, and inexpensive diagnostic assay alternative. Furthermore, we report here the identification of several new diagnostic antigens and potential subunit vaccine candidates for the highly infectious category B alphaproteobacteria, C. burnetii.
authors.affiliation: Department of Medicine, Division of Infectious Diseases, University of California, Irvine, CA 92697, USA. vigila@uci.edu
authors.forename: Adam
authors.initials: A
authors.lastname: Vigil
authors.forename: Rocio
authors.initials: R
authors.lastname: Ortega
authors.forename: Rie
authors.initials: R
authors.lastname: Nakajima-Sasaki
authors.forename: Jozelyn
authors.initials: J
authors.lastname: Pablo
authors.forename: Douglas M
authors.initials: DM
authors.lastname: Molina
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Chao
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Chen
authors.forename: Wei-Mei
authors.initials: WM
authors.lastname: Ching
authors.forename: Philip L
authors.initials: PL
authors.lastname: Felgner
mesh_terms: D016997:Coxiella burnetii / Q000235:genetics / Q000276:immunology*; D020869:Gene Expression Profiling; D006801:Humans; D056724:Immunity, Humoral / Q000235:genetics* / Q000276:immunology*; D016366:Open Reading Frames / Q000235:genetics; D016133:Polymerase Chain Reaction; D040081:Protein Array Analysis / Q000379:methods*; D011778:Q Fever / Q000276:immunology* / Q000382:microbiology
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