title: [RAPD analysis for genetic diversity of medicinal plant Coptis omeiensis].
abstract: OBJECTIVE
  To discuss the genetic diversity of Coptis omeiensis.
  
  
  METHODS
  The genetic diversity of 110 individuals from 10 populations was analyzed by RAPD.
  
  
  RESULTS
  14 primers were selected to produce highly reproducible RAPD bands. Among 132 amplified bands, 98 showed polymorphism, the percentage of polymorphic bands reached to 74.24%. Nei's gene diversity index (H) was 0.2863, Shannon's information index (I) was 0.3624, G(st) was 0.2305. The genetic distance coefficient and the similarity were 0.1931-0.5245 and 0.5016-0.8843, respectively.
  
  
  CONCLUSIONS
  There exists a held high genetic diversity in C. omeiensis and the majority of genetic variation occurs in the populations. By cluster analysis, the geographical distribution is very obvious. The RAPD marker can be used for the analysis of the genetic diversity and genetic variation of C. omeiensis.
authors.affiliation: School of Life Sciences, Southwest University, Key Laboratory (Ministry of Education) of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and Resources Research for Three Gorges Reservoir Region, Chongqing 400715, China. chunpingzhang520@163.com
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.forename: Junxing
authors.initials: J
authors.lastname: He
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuanbao
authors.initials: Y
authors.lastname: Qiao
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Zhangtian
authors.initials: Z
authors.lastname: Shi
authors.forename: Shijun
authors.initials: S
authors.lastname: Hu
mesh_terms: D002681:China; D039323:Coptis / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique