title: Modulation of human vascular endothelial cell behaviors by nanotopographic cues.
abstract: Basement membranes possess a complex three-dimensional topography in the nanoscale and submicron range which have been shown to profoundly modulate a large menu of fundamental cell behaviors. Using the topographic features found in native vascular endothelial basement membranes as a guide, polyurethane substrates were fabricated containing anisotropically ordered ridge and groove structures and isotropically ordered pores from 200 nm to 2000 nm in size. We investigated the impact of biomimetic length-scale topographic cues on orientation/elongation, proliferation and migration on four human vascular endothelial cell-types from large and small diameter vessels. We found that all cell-types exhibited orientation and alignment with the most pronounced response on anisotropically ordered ridges > or =800 nm. HUVEC cells were the only cell-type examined to demonstrate a decrease in proliferation in response to the smallest topographic features regardless of surface order. On anisotropically ordered surfaces all cell-types migrated preferentially parallel to the long axis of the ridges, with the greatest increase in cell migration being observed on the 1200 nm pitch. In contrast, cells did not exhibit any preference in direction or increase in migration speed on isotropically ordered surfaces. Overall, our data demonstrate that surface topographic features impact vascular endothelial cell behavior and that the impact of features varies with the cell behavior being considered, topographic feature scale, surface order, and the anatomic origin of the cell being investigated.
authors.affiliation: Department of Chemical and Biological Engineering, School of Engineering, University of Wisconsin-Madison, USA.
authors.forename: Sara J
authors.initials: SJ
authors.lastname: Liliensiek
authors.forename: Joshua A
authors.initials: JA
authors.lastname: Wood
authors.forename: Jiang
authors.initials: J
authors.lastname: Yong
authors.forename: Robert
authors.initials: R
authors.lastname: Auerbach
authors.forename: Paul F
authors.initials: PF
authors.lastname: Nealey
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Murphy
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size
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