title: Comparative transcriptome analysis of green/white variegated sectors in Arabidopsis yellow variegated2: responses to oxidative and other stresses in white sectors.
abstract: The yellow variegated2 (var2) mutant in Arabidopsis thaliana has been studied as a typical leaf-variegated mutant whose defect results from the lack of FtsH2 metalloprotease in chloroplasts. The var2 green sectors suffer from photo-oxidative stress and accumulate high levels of reactive oxygen species (ROS) because of compromised Photosystem II repair. This study investigated and compared microarray-based expression profiles of green and white sectors of var2 leaves. Results suggest that ROS that accumulate in chloroplasts of var2 green sectors do not cause much significant change in the transcriptional profile related to ROS signalling and scavenging. By contrast, transcriptome in the white sectors apparently differs from those in the green sectors and wild type. Numerous genes related to photosynthesis and chloroplast functions were repressed in the white sectors. Furthermore, many genes related to oxidative stress were up-regulated. Among them, ROS scavenging genes were specifically examined, such as Cu/Zn superoxide dismutase 2 (CSD2), that were apparently up-regulated in white but not in the green sectors. Up-regulation of CSD2 appears to be partly attributable to the lack of a microRNA (miR398) in the white sectors. It was concluded that the white sectors exhibit a response to oxidative and other stresses, including CSD2 up-regulation, which might be commonly found in tissues with abnormal chloroplast differentiation.
authors.affiliation: Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
authors.forename: Eiko
authors.initials: E
authors.lastname: Miura
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kato
authors.forename: Wataru
authors.initials: W
authors.lastname: Sakamoto
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018384:Oxidative Stress; D010858:Pigmentation; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins
references.citation: Plant J. 2003 Aug;35(4):512-22
references.pmid: 12904213
references.citation: Plant Physiol. 2009 Jun;150(2):670-83
references.pmid: 19363092
references.citation: Plant Cell. 2005 Oct;17(10):2782-90
references.pmid: 16126834
references.citation: Curr Opin Plant Biol. 2006 Jun;9(3):234-40
references.pmid: 16603408
references.citation: Plant Cell Physiol. 2000 Dec;41(12):1334-46
references.pmid: 11134419
references.citation: Plant Physiol. 2007 Jun;144(2):952-60
references.pmid: 17449646
references.citation: J Biol Chem. 2007 Jun 1;282(22):16369-78
references.pmid: 17405879
references.citation: Ann Bot. 2010 May;105(5):811-22
references.pmid: 19482877
references.citation: Plant Physiol. 2009 Dec;151(4):1790-801
references.pmid: 19767385
references.citation: Ann Bot. 2006 Aug;98(2):279-88
references.pmid: 16740587
references.citation: Photosynth Res. 2007 Jan;91(1):11-23
references.pmid: 17342448
references.citation: Plant Cell. 2006 Aug;18(8):2051-65
references.pmid: 16861386
references.citation: Plant Physiol. 2006 Jun;141(2):391-6
references.pmid: 16760493
references.citation: Cell Res. 2009 Oct;19(10):1205-16
references.pmid: 19581937
references.citation: Plant Physiol. 1998 Oct;118(2):637-50
references.pmid: 9765550
references.citation: J Exp Bot. 2002 May;53(372):1305-19
references.pmid: 11997377
references.citation: Anal Biochem. 1971 Nov;44(1):276-87
references.pmid: 4943714
references.citation: Plant Cell. 2009 Jan;21(1):347-61
references.pmid: 19122104
references.citation: Genes Genet Syst. 2003 Feb;78(1):1-9
references.pmid: 12655133
references.citation: Plant Cell. 2007 Apr;19(4):1313-28
references.pmid: 17416734
references.citation: Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12826-31
references.pmid: 11606728
references.citation: J Biochem. 2009 Oct;146(4):463-9
references.pmid: 19451147
references.citation: Plant Physiol. 2009 Jun;150(2):904-23
references.pmid: 19386811
references.citation: Annu Rev Plant Biol. 2006;57:599-621
references.pmid: 16669775
references.citation: Nat Genet. 2000 May;25(1):25-9
references.pmid: 10802651
references.citation: Plant J. 2000 May;22(4):303-13
references.pmid: 10849347
references.citation: Trends Plant Sci. 2002 Sep;7(9):405-10
references.pmid: 12234732
references.citation: Plant Cell. 2005 Jan;17(1):268-81
references.pmid: 15608336
references.citation: Annu Rev Plant Biol. 2004;55:373-99
references.pmid: 15377225
references.citation: Plant Cell. 2003 Dec;15(12):2843-55
references.pmid: 14630971
references.citation: Biochem J. 2001 Nov 1;359(Pt 3):575-82
references.pmid: 11672431
references.citation: Plant Physiol. 1994 Sep;106(1):71-77
references.pmid: 12232304
references.citation: Plant Cell Physiol. 2009 Dec;50(12):2069-83
references.pmid: 19755395
references.citation: Genes Cells. 2002 Aug;7(8):769-80
references.pmid: 12167156
references.citation: J Exp Bot. 2005 Jan;56(411):357-63
references.pmid: 15545296
references.citation: Planta. 2008 Jan;227(2):465-75
references.pmid: 17909854
references.citation: Plant Cell. 2003 Jun;15(6):1333-46
references.pmid: 12782727
references.citation: Plant Cell. 2008 Jul;20(7):1786-804
references.pmid: 18599582
references.citation: Plant Cell. 2005 Mar;17(3):957-70
references.pmid: 15705948
references.citation: Plant Cell. 2005 Jul;17(7):1866-75
references.pmid: 15987996
references.citation: Biosci Biotechnol Biochem. 2008 May;72(5):1143-54
references.pmid: 18460785
references.citation: Plant Cell. 2009 Jun;21(6):1781-97
references.pmid: 19531596
references.citation: Plant Cell. 2005 Apr;17(4):1233-51
references.pmid: 15772282
references.citation: Plant Physiol. 2005 Jul;138(3):1289-300
references.pmid: 16010003
references.citation: J Biol Chem. 2002 Jan 18;277(3):2006-11
references.pmid: 11717304
references.citation: Plant Cell. 2008 Nov;20(11):3148-62
references.pmid: 18996978
references.citation: Biochem Biophys Res Commun. 2009 Aug 14;386(1):6-10
references.pmid: 19501049
references.citation: J Biol Chem. 2008 Jun 6;283(23):15932-45
references.pmid: 18408011
references.citation: Trends Plant Sci. 2002 Jan;7(1):14-21
references.pmid: 11804822