Transverse aortic constriction in mice.
Transverse aortic constriction (TAC) in the mouse is a commonly used experimental model for pressure overload-induced cardiac hypertrophy and heart failure. TAC initially leads to compensated hypertrophy of the heart, which often is associated with a temporary enhancement of cardiac contractility. Over time, however, the response to the chronic hemodynamic overload becomes maladaptive, resulting in cardiac dilatation and heart failure. The murine TAC model was first validated by Rockman et al., and has since been extensively used as a valuable tool to mimic human cardiovascular diseases and elucidate fundamental signaling processes involved in the cardiac hypertrophic response and heart failure development. When compared to other experimental models of heart failure, such as complete occlusion of the left anterior descending (LAD) coronary artery, TAC provides a more reproducible model of cardiac hypertrophy and a more gradual time course in the development of heart failure. Here, we describe a step-by-step procedure to perform surgical TAC in mice. To determine the level of pressure overload produced by the aortic ligation, a high frequency Doppler probe is used to measure the ratio between blood flow velocities in the right and left carotid arteries. With surgical survival rates of 80-90%, transverse aortic banding is an effective technique of inducing left ventricular hypertrophy and heart failure in mice.
deAlmeida
Angela C
AC
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, USA.
van Oort
Ralph J
RJ
Wehrens
Xander H T
XH
D000818:Animals; D001013:Aorta, Thoracic / Q000473:pathology / Q000601:surgery*; D006332:Cardiomegaly / Q000209:etiology*; D013504:Cardiovascular Surgical Procedures / Q000379:methods; D003251:Constriction, Pathologic; D004195:Disease Models, Animal; D006333:Heart Failure / Q000209:etiology*; D006801:Humans; D008026:Ligation / Q000379:methods; D051379:Mice
J Gerontol A Biol Sci Med Sci. 2003 Oct;58(10):B895-9
14570855
Nat Rev Mol Cell Biol. 2006 Aug;7(8):589-600
16936699
Hypertension. 2010 Apr;55(4):932-8
20157052
IEEE Trans Biomed Eng. 2005 Oct;52(10):1771-83
16235663
J Biol Chem. 2008 Aug 8;283(32):22295-303
18477567
Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8277-81
1832775
Ultrasound Med Biol. 2008 Jun;34(6):892-901
18255218
Physiol Genomics. 2004 Feb 13;16(3):349-60
14679301