Dexamethasone acutely accelerates pleural fluid absorption in mice hydrothoraces.
This study assessed the effect of corticosteroid treatment in the clearance of hydrothoraces in mice. Twenty-four C57BL/6 mice were divided into four groups and were injected intrapleurally with 500 microL sterilized PBS-BSA 1% to create isosmotic hydrothoraces. Two groups served as control and two groups were treated with dexamethasone. The control groups received intraperitoneally PBS, while the corticosteroid treatment groups received dexamethasone (1 mg/kg), both 5 min after the induction of hydrothorax. Control and treated animals were sacrificed 2 and 4 h after the induction of hydrothorax, and pleural fluid volume was measured. The pleural fluid volume 2 and 4 h after the induction of hydrothoraces was significantly lower in the dexamethasone-treated group compared to the untreated group. The rate of pleural fluid absorption 2 and 4 h after the induction of hydrothoraces was significantly higher in the dexamethasone-treated groups. The present study demonstrated that dexamethasone accelerates pleural fluid absorption in induced isosmotic hydrothoraces in mice. This newly reported property of dexamethasone may partly account for the clinical observation of faster resolution of pleural effusions when corticosteroids are administered in patients with pleural effusions of certain etiologies.
Zarogiannis
Sotirios
S
Department of Physiology, Medical School, University of Thessaly, and Department of Respiratory Medicine, University Hospital of Larissa, Mezourlo Hill, 41110, Larissa, Greece. szarog@med.uth.gr
Psallidas
Ioannis
I
Hatzoglou
Chrissi
C
Gourgoulianis
Konstantinos I
KI
Kalomenidis
Ioannis
I
D000305:Adrenal Cortex Hormones / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D006876:Hydrothorax / Q000188:drug therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D010996:Pleural Effusion / Q000188:drug therapy*
D000305:Adrenal Cortex Hormones; D003907:Dexamethasone
Am J Physiol Lung Cell Mol Physiol. 2006 Dec;291(6):L1220-31
16877633
Am J Physiol Cell Physiol. 2000 Dec;279(6):C1744-50
11078688
Eur J Pharmacol. 2006 Apr 24;536(1-2):12-8
16564041
Adv Perit Dial. 2008;24:2-6
18985993
Am J Physiol Lung Cell Mol Physiol. 2007 Nov;293(5):L1332-8
17873005
Am J Respir Cell Mol Biol. 2009 May;40(5):543-54
18927349
Cochrane Database Syst Rev. 2007 Oct 17;(4):CD001876
17943759
Curr Opin Pulm Med. 2003 Jul;9(4):254-60
12806236
Respir Physiol Neurobiol. 2007 Dec 15;159(3):311-23
17884738
Physiol Rev. 2004 Apr;84(2):385-410
15044678
Respir Med. 1996 Sep;90(8):501-3
8869446
Respir Physiol Neurobiol. 2003 Dec 16;139(1):83-8
14637313
Chest. 2001 May;119(5):1547-62
11348966
Eur J Pharmacol. 1993 Jun 1;248(1):41-7
8339753
J Physiol Sci. 2009 Jul;59(4):335-9
19462220
Respirology. 2008 Jan;13(1):73-8
18197914
Am J Physiol. 1997 Oct;273(4):L825-30
9357858