Laser-induced propagation and destruction of amyloid beta fibrils.
The amyloid deposition of amyloid beta (Abeta) peptides is a critical pathological event in Alzheimer disease (AD). Preventing the formation of amyloid deposits and removing preformed fibrils in tissues are important therapeutic strategies against AD. Previously, we reported the destruction of amyloid fibrils of beta(2)-microglobulin K3 fragments by laser irradiation coupled with the binding of amyloid-specific thioflavin T. Here, we studied the effects of a laser beam on Abeta fibrils. As was the case for K3 fibrils, extensive irradiation destroyed the preformed Abeta fibrils. However, irradiation during spontaneous fibril formation resulted in only the partial destruction of growing fibrils and a subsequent explosive propagation of fibrils. The explosive propagation was caused by an increase in the number of active ends due to breakage. The results not only reveal a case of fragmentation-induced propagation of fibrils but also provide insights into therapeutic strategies for AD.
Yagi
Hisashi
H
Institute for Protein Research, Osaka University, Yamadaoka 3-2, Suita, Osaka 565-0871, Japan.
Ozawa
Daisaku
D
Sakurai
Kazumasa
K
Kawakami
Toru
T
Kuyama
Hiroki
H
Nishimura
Osamu
O
Shimanouchi
Toshinori
T
Kuboi
Ryoichi
R
Naiki
Hironobu
H
Goto
Yuji
Y
D000544:Alzheimer Disease / Q000378:metabolism / Q000628:therapy*; D000682:Amyloid / Q000737:chemistry*; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D052160:Benzothiazoles; D006801:Humans; D007700:Kinetics; D007834:Lasers; D008954:Models, Biological; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010778:Photochemotherapy / Q000379:methods; D017510:Protein Folding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013844:Thiazoles / Q000737:chemistry; D014461:Ultracentrifugation; D001613:beta 2-Microglobulin / Q000737:chemistry
D000682:Amyloid; D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D010446:Peptide Fragments; D010455:Peptides; D013844:Thiazoles; D001613:beta 2-Microglobulin; C009462:thioflavin T
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