title: Nocardioides hungaricus sp. nov., isolated from a drinking water supply system.
abstract: Three Gram-positive, rod-shaped bacterial strains were isolated from the drinking water supply system of the Hungarian capital, Budapest. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparison revealed that the isolates represented a distinct cluster within the clade of the genus Nocardioides and were most closely related to Nocardioides pyridinolyticus OS4(T), Nocardioides aquiterrae GW-9(T), Nocardioides sediminis MSL-01(T) and N. hankookensis DS-30(T). The peptidoglycan based on LL-2,6-diaminopimelic acid, the major menaquinone MK-8(H₄), the cellular fatty acid profile with iso-C₁₆:₀ and anteiso-C₁₇:₀ as predominating components and the DNA G+C content of 71.4 mol% (strain 1RaM5-12(T)) were consistent with the affiliation of the isolates to the genus Nocardioides. Because of differences in physiological characteristics, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of protein extracts, PvuII RiboPrinter patterns and 96.1 % 16S rRNA gene sequence similarity between strain 1RaM5-12(T) and its closest phylogenetic neighbour, N. pyridinolyticus OS4(T), a novel species, Nocardioides hungaricus sp. nov., is proposed. The type strain is 1RaM5-12(T) (=DSM 21673(T) =NCAIM 02330(T)).
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary.
authors.forename: Erika M
authors.initials: EM
authors.lastname: Tóth
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary.
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Kéki
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Makk
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary.
authors.forename: Zalán G
authors.initials: ZG
authors.lastname: Homonnay
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary.
authors.forename: Károly
authors.initials: K
authors.lastname: Márialigeti
authors.affiliation: DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid; D005227:Fatty Acids; D006814:Hungary; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2; D014871:Water Microbiology; D014881:Water Supply
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid