Allergen-specific IL-10-secreting type I T regulatory cells, but not CD4(+)CD25(+)Foxp3(+) T cells, are decreased in peripheral blood of patients with persistent allergic rhinitis.
We investigate the frequencies of CD4(+)CD25(+)Foxp3(+) T cells and allergen-specific IL-10(+)IL-4(-), IFN-gamma(+)IL-4(-), IL-4(+)IFN-gamma(-)CD4(+) T cells (which display characteristics of nTreg, Tr1-, Th1- and Th2- cells, respectively) in peripheral blood mononuclear cells (PBMCs) of patients with AR and of healthy individuals. In addition, we estimated the suppressive effect of CD4(+)CD25(+) Treg cells and allergen-specific, IL-10-secreting cells from both two groups. The frequency of CD4(+)CD25(+)Foxp3(+) T cells is similar in 43 AR patients compared with 38 healthy subjects. CD4(+)CD25(high) cells retain suppressive activity on allergen-stimulated cell proliferation and cytokine production of Th1 but not Th2 cells in both groups. However, the frequency of allergen-specific IL-10(+)IL-4(-)CD4(+) T cells is reduced in AR patients, and correlates inversely to clinical symptom scores. Allergen-specific, IL-10-secreting cells potently suppressed D. pteronyssinus major allergen 1-stimulated cell proliferation and cytokine production (IFN-gamma and IL-4) in healthy individuals. Altogether our data indicate that the number and function of CD4(+)CD25(+) Treg cells from allergic patients are not impaired. However, the deficiency of allergen-specific Tr1 cells may play a role in the development of AR.
Han
Demin
D
Department of Otolaryngology, Beijing TongRen Hospital, Capital Medical University, Beijing, China.
Wang
Chengshuo
C
Lou
Wei
W
Gu
Yili
Y
Wang
Yang
Y
Zhang
Luo
L
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*; D018417:Th1 Cells; D018418:Th2 Cells; D055815:Young Adult
D000485:Allergens; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit; D016753:Interleukin-10