title: Analysis of cargo transport by IFT and GFP imaging of IFT in Chlamydomonas.
abstract: Chlamydomonas reinhardtii is the organism in which intraflagellar transport (IFT) was first visualized and in which the composition of IFT particles was originally elucidated. As the universality of IFT among ciliated/flagellated cells was uncovered, the diversity of organisms used to study IFT has grown. Still, because of the ease of isolation of flagella from Chlamydomonas and the battery of temperature-sensitive mutants affecting IFT proteins and motors, this unicellular alga remains the principal model for biochemical studies of IFT motors and cargo; furthermore, the long, exposed flagella of this cell are ideally suited for observing IFT in real time with GFP-tagged components of IFT.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
authors.forename: Dennis
authors.initials: D
authors.lastname: Diener
mesh_terms: D000818:Animals; D054468:Axoneme / Q000378:metabolism / Q000648:ultrastructure; D001692:Biological Transport / Q000502:physiology; D016825:Chlamydomonas reinhardtii / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D005407:Flagella / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
references.citation: Methods Enzymol. 1986;134:280-90
references.pmid: 3821567
references.citation: Nature. 1999 Apr 22;398(6729):674
references.pmid: 10227290
references.citation: Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5519-23
references.pmid: 8516294
references.citation: J Cell Biol. 2001 Jun 11;153(6):1315-26
references.pmid: 11402073
references.citation: Curr Biol. 2007 Feb 6;17(3):193-202
references.pmid: 17276912
references.citation: J Cell Biol. 1978 Jul;78(1):8-27
references.pmid: 149796
references.citation: Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4457-62
references.pmid: 9114011
references.citation: J Cell Biol. 2009 Aug 24;186(4):601-13
references.pmid: 19704024
references.citation: J Cell Biol. 1996 Apr;133(2):371-9
references.pmid: 8609169
references.citation: J Cell Biol. 2005 Aug 15;170(4):649-59
references.pmid: 16103230
references.citation: Methods Cell Biol. 1995;47:9-12
references.pmid: 7476552
references.citation: J Cell Biol. 1995 Dec;131(6 Pt 1):1517-27
references.pmid: 8522608
references.citation: Mol Biol Cell. 2005 Mar;16(3):1341-54
references.pmid: 15616187
references.citation: J Cell Biol. 2007 Nov 5;179(3):501-14
references.pmid: 17984324
references.citation: J Cell Biol. 2004 Jan 19;164(2):255-66
references.pmid: 14718520
references.citation: J Cell Sci. 2006 Mar 15;119(Pt 6):1165-74
references.pmid: 16507594
references.citation: Methods Cell Biol. 1995;47:263-71
references.pmid: 7476498
references.citation: J Cell Sci. 2003 Jun 1;116(Pt 11):2179-86
references.pmid: 12692152
references.citation: J Cell Biol. 1972 Sep;54(3):507-39
references.pmid: 4558009
references.citation: Plant J. 1999 Aug;19(3):353-61
references.pmid: 10476082
references.citation: J Cell Biol. 2001 Apr 2;153(1):13-24
references.pmid: 11285270
references.citation: J Cell Biol. 2007 Feb 26;176(5):653-65
references.pmid: 17312020