Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL).
The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for LFucalpha1--> and related complexes and for high density Manalpha1--> complexes present in polymannosylated target macromolecules.
Wu
Albert M
AM
Institute of Molecular and Cellular Biology, College of Medicine, Chang-Gung University, Kwei-san, Tao-yuan, Taiwan. amwu@mail.cgu.edu.tw
Gong
Yu-Ping
YP
Li
Chia-Chen
CC
Gilboa-Garber
Nechama
N
D018829:Adhesins, Bacterial / Q000378:metabolism*; D002241:Carbohydrates / Q000235:genetics*; D037102:Lectins / Q000235:genetics* / Q000378:metabolism*; D008024:Ligands; D008968:Molecular Conformation; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
D018829:Adhesins, Bacterial; D002241:Carbohydrates; D037102:Lectins; D008024:Ligands; C038692:adhesin, Pseudomonas