title: Effect of stretching force on the cells of epithelial rests of malassez in vitro.
abstract: Background and Objective. The aim of this study was to investigate the behavior of cells from epithelial rest of Malassez (ERM) against stretching force. Material and Methods. ERM-cultured cells were stretched for 1 hour, at the cycle of 18% elongation for 1 second followed by 1-second relaxation. The cells without addition of stretching force were used as controls. The cells were observed by immunohistochmical staining using actin 0, 12, 24, 36, 48, and 72 hours. Furthermore, expressions of HSP70-, VEGF-, and OPN-mRNAs of cells were also evaluated using quantitative RT-PCR. Results. Actin filaments were randomly orientated in the cytoplasm in the control group, whereas in the stretching group, actin filaments were orientated comparatively parallel to the stretching direction. Expression of HSP70-mRNA in the stretching group was significantly higher than that of control group at 12, 24, 36 hours (P < .05). Expression of VEGF-mRNA in the stretching group was significantly higher than that of control group at 24, 36, 48, and 72 hours (P < .05). Expression of OPN-mRNA in the stretching group was significantly higher than that of control group at 12 and 24 hours (P < .05). Conclusion. ERM cells response against the stretching force by expressing HSP70, VEGF, and OPN.
authors.affiliation: Department of Crown & Bridge Prosthodontics, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba 261-8502, Japan.
authors.forename: Teruyoshi
authors.initials: T
authors.lastname: Koshihara
authors.forename: Kenichi
authors.initials: K
authors.lastname: Matsuzaka
authors.forename: Toru
authors.initials: T
authors.lastname: Sato
authors.forename: Takashi
authors.initials: T
authors.lastname: Inoue
references.citation: Crit Rev Oral Biol Med. 2000;11(3):279-303
references.pmid: 11021631
references.citation: J Cell Sci. 1984 Jul;69:35-45
references.pmid: 6490748
references.citation: Laryngoscope. 1995 Dec;105(12 Pt 1):1300-4
references.pmid: 8523981
references.citation: Microsc Res Tech. 2003 Apr 1;60(5):491-502
references.pmid: 12619125
references.citation: Science. 1989 Dec 8;246(4935):1306-9
references.pmid: 2479986
references.citation: Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):233-5
references.pmid: 7542074
references.citation: J Orthop Res. 1994 Jan;12(1):70-8
references.pmid: 8113944
references.citation: J Periodontol. 2008 May;79(5):912-9
references.pmid: 18454671
references.citation: Eur J Oral Sci. 2003 Aug;111(4):332-8
references.pmid: 12887399
references.citation: Exp Nephrol. 1994 May-Jun;2(3):158-65
references.pmid: 7922267
references.citation: J Appl Physiol (1985). 2000 Dec;89(6):2498-507
references.pmid: 11090608
references.citation: Cell Biol Int. 2005 Feb;29(2):111-7
references.pmid: 15774307
references.citation: J Periodontal Res. 2006 Aug;41(4):245-52
references.pmid: 16827716
references.citation: Oral Surg Oral Med Oral Pathol. 1972 Dec;34(6):956-64
references.pmid: 4509005
references.citation: Exp Cell Res. 1980 Jun;127(2):470-4
references.pmid: 7379874
references.citation: J Periodontol. 1980 Aug;51(8):437-44
references.pmid: 6931203
references.citation: Orthod Craniofac Res. 2004 Feb;7(1):26-34
references.pmid: 14989752
references.citation: Dev Dyn. 1995 May;203(1):80-92
references.pmid: 7647376
references.citation: J Periodontal Res. 2003 Feb;38(1):51-6
references.pmid: 12558937
references.citation: J Dent Res. 2000 Sep;79(9):1712-6
references.pmid: 11023268
references.citation: Arch Oral Biol. 1998 Dec;43(12):987-97
references.pmid: 9877330
references.citation: J Biochem Mol Toxicol. 2003;17(3):161-8
references.pmid: 12815612
references.citation: Eur J Oral Sci. 2008 Aug;116(4):312-8
references.pmid: 18705798
references.citation: J Periodontal Res. 2005 Apr;40(2):110-7
references.pmid: 15733145
references.citation: Scand J Gastroenterol. 2006 Mar;41(3):312-7
references.pmid: 16497619
references.citation: Arch Oral Biol. 1998 Nov;43(11):899-906
references.pmid: 9821513
references.citation: World J Gastroenterol. 2005 Sep 28;11(36):5725-31
references.pmid: 16237775
references.citation: Eur J Cell Biol. 1986 Aug;41(2):339-46
references.pmid: 3530766
references.citation: Arch Oral Biol. 1979;24(3):199-203
references.pmid: 289357
references.citation: Nat Cell Biol. 2000 Aug;2(8):469-75
references.pmid: 10934466
references.citation: J Anat. 1993 Apr;182 ( Pt 2):153-62
references.pmid: 8376190
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1996 Jun;81(6):703-9
references.pmid: 8784903
references.citation: FEBS Lett. 1993 May 24;323(1-2):1-3
references.pmid: 8495719
references.citation: Experientia. 1993 Aug 15;49(8):706-13
references.pmid: 8359277
references.citation: Crit Rev Oncog. 2000;11(2):135-46
references.pmid: 11005509
references.citation: Arch Oral Biol. 1989;34(3):179-85
references.pmid: 2818267
references.citation: Sci STKE. 2001 Jul 31;2001(93):re1
references.pmid: 11752668
references.citation: J Oral Rehabil. 2008 Dec;35(12):889-97
references.pmid: 19090907
references.citation: Arch Histol Cytol. 1989 Mar;52(1):61-70
references.pmid: 2497762
references.citation: J Periodontal Res. 2005 Oct;40(5):417-26
references.pmid: 16105095
references.citation: J Periodontol. 1986 Mar;57(3):164-71
references.pmid: 3457140