title: Isolation of scFv fragments specific for monokine induced by interferon-gamma (MIG) using phage display.
abstract: Iterative affinity selection procedures were used to isolate a number of single chain Fv (scFv) antibody fragment clones from naïve Tomlinson I+J phage display libraries that specifically recognize and bind a chemokine, monokine induced by interferon-gamma (MIG/CXCL9). MIG is an important transplant rejection/biology chemokine protein. ELISA-based affinity characterization results indicate that selectants preferentially bind to MIG in the presence of key biopanning component materials and closely related chemokine proteins. These novel antibody fragments may find utility as molecular affinity interface receptors in various electrochemical biosensor platforms to provide specific MIG binding capability with potential applications in transplant rejection monitoring, and other biomedical applications where detection of MIG level is important.
authors.affiliation: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43230, USA. Edward.Eteshola@osumc.edu
authors.forename: Edward
authors.initials: E
authors.lastname: Eteshola
mesh_terms: D000595:Amino Acid Sequence; D001667:Binding, Competitive / Q000276:immunology; D054370:Chemokine CXCL9 / Q000276:immunology*; D018925:Chemokines / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D019151:Peptide Library; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D057127:Single-Chain Antibodies / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*
chemical_list: C080810:CXCL9 protein, human; D054370:Chemokine CXCL9; D018925:Chemokines; D000939:Epitopes; D019151:Peptide Library; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies
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