Development of transgenic rice containing a mutated beta subunit of soybean beta-conglycinin for enhanced phagocytosis-stimulating activity.
Improving the nutraceutical value of rice would positively impact the health and well-being of rice consumers worldwide. Based on the three-dimensional structure of soybean beta-conglycinin, we designed a beta subunit with a strong phagocytosis-stimulating activity (mbeta subunit). Here, we describe the genetic modification and production of rice seeds containing the mbeta subunit as part of our aim to develop a food material that promotes human health. The mbeta subunit folded correctly and was accumulated in the protein body II of rice seeds at a level similar to wild-type beta subunit. Mutant beta subunit purified from transgenic rice seeds exhibited high phagocytosis-stimulating activity, demonstrating its potential value in enhancing the nutritional value of rice.
Motoyama
Takayasu
T
Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
Amari
Yoshiki
Y
Tandang-Silvas
Mary Rose
MR
Cabanos
Cerrone
C
Kimura
Aiko
A
Yoshikawa
Masaaki
M
Takaiwa
Fumio
F
Utsumi
Shigeru
S
Maruyama
Nobuyuki
N
D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000235:genetics*; D005916:Globulins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics*; D010587:Phagocytosis / Q000235:genetics*; D030821:Plants, Genetically Modified; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D055314:Seed Storage Proteins / Q000235:genetics*; D030262:Soybean Proteins / Q000235:genetics*
D052179:Antigens, Plant; D005916:Globulins; D021122:Protein Subunits; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max