Dangshen (Codonopsis pilosula) activates IGF-I and FGF-2 pathways to induce proliferation and migration effects in RSC96 Schwann cells.
This study evaluates the proliferative and migrative effects of dangshen on RSC96, Schwann cells. We investigated the molecular signaling pathways, which include: (1) survival signaling, IGFs-IGFIR-Akt-Bcl2 and proliferative signaling, cell cycle factors and MAPK pathways. (2) migrate and anti-scar signaling, FGF-2-uPA-MMPs. After treatment with different concentrations (20 microg/ml, 40 microg/ml, 60 microg/ml, 80 microg/ml, and 100 microg/ml) of dangshen. We observed a dose dependent proliferative effect using PCNA Western blotting assay, MTT assay and the wound healing test. We also found that dangshen stimulates the protein expressions of IGF-I pathway regulators, cell cycle controlling proteins and excites the MAPK signaling pathway regulators ERK and P38. Dangshen even stimulates the FGF-2-uPA-MMP 9 migration pathway in RSC 96 Schwann cells. Using MAPK chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of dangshen on RSC 96 cells were identified to be ERK- and P38- dependent. Based on these results, applying an appropriate dose of dangshen with biomedical materials would be a potential approach for enhancing neuron regeneration.
Chen
Hsien-Tung
HT
Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan.
Tsai
Ying-Lan
YL
Chen
Yueh-Sheng
YS
Jong
Guo-Ping
GP
Chen
Wei-Kung
WK
Wang
Hwai-Lee
HL
Tsai
Fuu-Jen
FJ
Tsai
Chang-Hai
CH
Lai
Tung-Yuan
TY
Tzang
Bor-Show
BS
Huang
Chih-Yang
CY
Lu
Chung-Yen
CY
D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D029745:Codonopsis / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D010936:Plant Extracts; D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I