Rapid diagnosis of Old World Leishmaniasis by high-resolution melting analysis of the 7SL RNA gene.
High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World Leishmania was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (P < 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.
Nasereddin
Abedelmajeed
A
Department of Microbiology and Molecular Genetics, IMIRC, the Kuvin Center for the Study of Infectious and Tropical Diseases, the Hebrew University-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel.
Jaffe
Charles L
CL
D017931:DNA Primers / Q000235:genetics; D006801:Humans; D007891:Leishmania / Q000235:genetics* / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000469:parasitology; D010274:Parasitology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016053:RNA, Protozoan / Q000235:genetics*; D020733:RNA, Small Cytoplasmic / Q000235:genetics*; D012680:Sensitivity and Specificity; D018271:Signal Recognition Particle / Q000235:genetics*; D044366:Transition Temperature
C115990:7SL RNA; D017931:DNA Primers; D016053:RNA, Protozoan; D020733:RNA, Small Cytoplasmic; D018271:Signal Recognition Particle
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