Involvement of recF in 254 nm ultraviolet radiation resistance in Deinococcus radiodurans and Escherichia coli.
recF is a critical gene involved in the RecFOR pathway of DNA repair in Deinococcus radiodurans (D. radiodurans). To investigate the role of recF in UV-C radiation resistance, we generated the recF-deficient D. radiodurans strain R1, expressed recF in Escherichia coli (E. coli) BL21 cells, and compared the ability of each to resist UV-C radiation by F (10), inactivation constant (IC), and extrapolation number (N). The mutation of recF in D. radiodurans R1 resulted in characteristic slow growth and dramatic sensitivity to UV-C irradiation. Transformation of recF into E. coli BL21 cells resulted in increased UV-C resistance compared to untransformed E. coli BL21 cells. These results suggested that recF is needed for the replication of D. radiodurans. Furthermore, as a part of the RecFOR pathway in D. radiodurans, disruption of recF could dramatically decrease the UV-C resistance of D. radiodurans and recF could increase the UV-C resistance of E. coli BL21 cells.
Chang
Xiaosong
X
Department of Environmental Hygiene, Third Military Medical University, Chongqing, China.
Yang
Lan
L
Zhao
Qing
Q
Fu
Wenjuan
W
Chen
Hao
H
Qiu
Zhiqun
Z
Chen
Ji-an
JA
Hu
Ran
R
Shu
Weiqun
W
D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology*; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D034301:Deinococcus / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D005784:Gene Amplification; D042822:Genomic Instability; D009154:Mutation; D016133:Polymerase Chain Reaction; D011836:Radiation Tolerance / Q000235:genetics*; D011995:Recombination, Genetic; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C066218:recF protein, Bacteria
J Biol Chem. 2004 Jan 30;279(5):3492-6
14625283
FEMS Microbiol Ecol. 2005 Jan 1;51(2):231-6
16329871
Mutat Res. 2000 Nov 9;461(3):179-87
11056289
J Bacteriol. 2005 Jun;187(11):3693-7
15901692
Arch Microbiol. 2009 Dec;191(12):913-8
19882142
J Bacteriol. 2002 Nov;184(22):6216-24
12399492
J Cell Biol. 2005 Aug 1;170(3):357-66
16061691
J Mol Biol. 2006 Sep 15;362(2):241-58
16916523
DNA Repair (Amst). 2008 Mar 1;7(3):353-79
17951115
Microbiol Mol Biol Rev. 1999 Dec;63(4):751-813, table of contents
10585965
Nat Rev Microbiol. 2005 Nov;3(11):882-92
16261171
Mutat Res. 2008 May 10;641(1-2):48-53
18343459
J Bacteriol. 2008 Apr;190(7):2624-8
18223077
Biochemistry. 2007 Aug 7;46(31):9080-8
17630776
PLoS One. 2007 Sep 26;2(9):e928
17895969
DNA Repair (Amst). 2008 Sep 1;7(9):1589-602
18644471
Curr Opin Struct Biol. 2003 Apr;13(2):249-55
12727520
Mutat Res. 2008 Oct 14;645(1-2):19-26
18782580
Mol Cell. 2003 May;11(5):1337-47
12769856
EMBO J. 2007 Feb 7;26(3):867-77
17255941
Nucleic Acids Res. 2008 Jan;36(1):94-109
18000001
Structure. 2007 Oct;15(10):1316-24
17937920
PLoS Genet. 2005 Aug;1(2):e15
16132081
Int J Radiat Biol. 2005 Dec;81(12):919-27
16524847
Science. 1999 Nov 19;286(5444):1571-7
10567266
Can J Microbiol. 2009 Jul;55(7):841-8
19767856
Microbiology (Reading). 2009 Jul;155(Pt 7):2320-2332
19389761
Annu Rev Microbiol. 1997;51:203-24
9343349
Cell. 1997 Oct 31;91(3):347-56
9363943
Mutat Res. 1999 Oct 22;435(2):151-61
10556595
J Biol Chem. 2006 Nov 24;281(47):36317-26
16968710
Mutat Res. 1999 Dec 7;435(3):171-213
10606811
Photochem Photobiol. 2008 Sep-Oct;84(5):1084-90
18627518