[Molecular cloning and sequence analysis of lactate dehydrogenase gene from Plasmodium vivax Hainan isolate].
Full length sequence of lactate dehydrogenase gene was amplified by PCR from the genomic DNA of Plasmodium vivax Hainan isolate, and named as PvLDH/HN (GenBank No. FJ527750). Sequence analysis showed that the gene had 951 bp, coding 316 aa. Compared with PvLDH/Salvador and PvLDH/Belem, the nucleotide sequence homology of PvLDH/HN was both 99.89%, while the homology of amino acid sequence was 100%. Topology analysis showed that the protein had two transmembrane alpha-helices, which suggested that the protein might be a membrane protein. The major antigen epitope regions (82-95aa) was presented on the protein surface, and formed the specific substrate binding loop, which revealed that it might be an ideal target site for drug action and immunodiagnosis.
Lv
Gang
G
Department of Parasitology, Hainan Medical College, Haikou 571101, China. luganghn@yahoo.com.cn
Li
Jing-jing
JJ
Zhao
Shi-yong
SY
Lu
Ya-jun
YJ
Fan
Zhi-gang
ZG
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000201:enzymology* / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D016054:DNA, Protozoan; D015800:Protozoan Proteins; D007770:L-Lactate Dehydrogenase