title: Zebrafish 10-formyltetrahydrofolate dehydrogenase is similar to its mammalian isozymes for its structural and catalytic properties.
abstract: 10-Formyltetrahydrofolate dehydrogenase from zebrafish has been cloned and expressed in both Escherichia coli and yeast. In addition, the N-terminal and C-terminal domains have also been cloned and expressed. Each expressed protein was purified to homogeneity and structural and kinetic properties determined. These studies show that the zebrafish enzyme is structurally and catalytically very similar to the enzymes from mammalian sources, suggesting that zebrafish can be used to study the in vivo function of 10-formyltetrahydrofolate dehydrogenase.
authors.affiliation: Institute of Basic Medical Science, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Wen-Ni
authors.initials: WN
authors.lastname: Chang
authors.forename: Hung-Chang
authors.initials: HC
authors.lastname: Lin
authors.forename: Tzu-Fun
authors.initials: TF
authors.lastname: Fu
mesh_terms: D000447:Aldehydes; D000818:Animals; D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002955:Leucovorin / Q000031:analogs & derivatives / Q000378:metabolism; D008297:Male; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010843:Pichia / Q000235:genetics; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish; D000099295:Formyltetrahydrofolate Dehydrogenase
chemical_list: D000447:Aldehydes; D007527:Isoenzymes; D002955:Leucovorin; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011994:Recombinant Proteins; D000099295:Formyltetrahydrofolate Dehydrogenase; C010161:10-formyltetrahydropteroylglutamic acid; C005556:propionaldehyde