title: Administration of thyroxine affects the morphometric parameters and VEGF expression in the uterus and placenta and the uterine vascularization but does not affect reproductive parameters in gilts during early gestation.
abstract: The aim of this study was to evaluate the effects of thyroxine administration on morphometric parameters, expression of vascular endothelial growth factor (VEGF) and vascularization in the uterus and placenta and reproductive parameters in gilts at 70 days of gestation. At 150 days of age, i.e., before first heat, 20 gilts were randomly divided into two experimental groups: treated (n=10) and control (n=10). The treated group received a daily dose of 400 μg of L-thyroxine (T(4)) in their diet until slaughter and the control group received only typical meals. Before artificial insemination, blood was collected to determine plasma total T(4). The gilts were inseminated in the second oestrus and slaughtered at 70 days of gestation. The foetal thyroid follicular epithelium height, number, size and weight of foetuses; foetal myogenesis, corpora lutea number, embryonic mortality rate, uterine weight, placental weight and placental fluid volume were measured. Histomorphometric and immunohistochemical analysis of uterus and placenta were determined. The averages of all variables were compared by the Student's t-test. The gilts treated with thyroxine showed significant increase of plasma total T(4). At 70 days of gestation, the heights of the trophoblastic epithelium, endometrial epithelium and endometrial gland epithelium were significantly higher in the group treated with T(4). The expression of cytoplasmatic and nuclear VEGF in trophoblastic cells and the number of blood vessels per field in endometrial stroma were significantly higher in the gilts treated with T(4). No other significant differences between groups were obtained with respect to other parameters (p>0.05). We conclude that oral administration of T(4) up to 70 days of pregnancy in gilts affects the morphometric parameters, the expression of placental VEGF and the uterine vascularization but does not affect reproductive parameters in gilts during early gestation.
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária da Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: Souza
authors.forename: N M
authors.initials: NM
authors.lastname: Ocarino
authors.forename: J F
authors.initials: JF
authors.lastname: Silva
authors.forename: J N
authors.initials: JN
authors.lastname: Boeloni
authors.forename: E F
authors.initials: EF
authors.lastname: Nascimento
authors.forename: I J
authors.initials: IJ
authors.lastname: Silva
authors.forename: R D
authors.initials: RD
authors.lastname: Castro
authors.forename: L P
authors.initials: LP
authors.lastname: Moreira
authors.forename: F R C L
authors.initials: FR
authors.lastname: Almeida
authors.forename: H
authors.initials: H
authors.lastname: Chiarini-Garcia
authors.forename: R
authors.initials: R
authors.lastname: Serakides
mesh_terms: D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D010920:Placenta / Q000033:anatomy & histology / Q000098:blood supply / Q000187:drug effects*; D011247:Pregnancy; D012098:Reproduction / Q000187:drug effects*; D013552:Swine; D013974:Thyroxine / Q000008:administration & dosage* / Q000097:blood; D014327:Trophoblasts; D014599:Uterus / Q000033:anatomy & histology / Q000098:blood supply / Q000187:drug effects*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D013974:Thyroxine