title: Determination of iodide in urine using electrospray ionization tandem mass spectrometry.
abstract: A rapid and sensitive electrospray ionization (ESI) tandem mass spectrometry (MS-MS) procedure was developed for the determination of iodide (I(-)). A gold (Au) and I(-) complex was formed immediately after the addition of the chelating agent NaAuCl(4) to I(-) solution, and was extracted with methyl isobutyl ketone. One to five microliters of the extract were injected directly into an ESI-MS-MS instrument. I(-) quantification was performed by selecting reaction monitoring of the product ion I(-) at m/z 127 derived from the precursor ion (197)AuI(2)(-) at m/z 451. I(-) concentration was measured in the quantification range from 10(-7) to 10(-5) M using 50 microL of solution within 10 min. Iodate was reduced to I(-) with ascorbic acid and determined. I(-) concentration in reference urine 2670a was measured after treatments.
authors.affiliation: Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan. kminakat@hama-med.ac.jp
authors.forename: Kayoko
authors.initials: K
authors.lastname: Minakata
authors.forename: Itaru
authors.initials: I
authors.lastname: Yamagishi
authors.forename: Sanae
authors.initials: S
authors.lastname: Kanno
authors.forename: Hideki
authors.initials: H
authors.lastname: Nozawa
authors.forename: Masako
authors.initials: M
authors.lastname: Suzuki
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
mesh_terms: D006801:Humans; D007454:Iodides / Q000652:urine*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007454:Iodides