Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans.
Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous d-lactate dehydrogenase expression.
Kovács
Akos T
AT
Groningen Biomolecular Sciences and Biotechnology Institute, Department of Genetics, University of Groningen, Kerklaan 30, 9751 NN Haren, Netherlands.
van Hartskamp
Mariska
M
Kuipers
Oscar P
OP
van Kranenburg
Richard
R
D001407:Bacillus / Q000235:genetics*; D001709:Biotechnology / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods; D055786:Gene Knockout Techniques / Q000379:methods; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D005827:Genetics, Microbial / Q000379:methods*; D045022:Lactate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins; D045022:Lactate Dehydrogenases; C071195:D-lactate dehydrogenase; D001616:beta-Galactosidase
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