Effects of insulin-like growth factor binding protein 7 on apoptosis in human teratocarcinoma cells in vitro.
Human teratocarcinoma cells (Tera-2) deprived of serum undergo programmed cell death which can be counteracted by simultaneous addition of IGF-II. This protective effect of IGF-II was specific in the sense that addition of IGF-binding protein 7 (IGFBP-7) resulted in an increased apoptotic rate almost comparable to that of the classical IGFBPs. Autoradiographic analysis of incorporated tritiated thymidine indicated that the proportion of S-phase cells was comparable, irrespective of total cell numbers. This further suggests that IGF-II rescues cells from apoptosis and that IGFBP-7 is a specific antagonist.
Engström
Wilhelm
W
Division of Pathology, Pharmacology and Toxicology, Department of Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, PO Box 7028, 75007 Uppsala, Sweden. wilhelm.engstrom@bvf.slu.se
D017209:Apoptosis / Q000187:drug effects*; D001345:Autoradiography; D048708:Cell Growth Processes / Q000187:drug effects / Q000502:physiology; D016895:Culture Media, Serum-Free; D006801:Humans; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000494:pharmacology; D018969:Insulin-Like Growth Factor Binding Proteins / Q000494:pharmacology*; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D018243:Teratocarcinoma / Q000473:pathology*
D016895:Culture Media, Serum-Free; D018971:Insulin-Like Growth Factor Binding Protein 2; D018969:Insulin-Like Growth Factor Binding Proteins; D011994:Recombinant Proteins; C102529:insulin-like growth factor binding protein-related protein 1; D007335:Insulin-Like Growth Factor II