Development and evaluation of porcine cysticercosis QuickELISA in Triturus EIA analyzer.
We evaluated three diagnostic antigens (recombinant GP50, recombinant T24H, and synthetic Ts18var1) for cysticercosis and found that all three performed well in detecting cysticercosis in humans and pigs in several assay formats. These antigens were adapted to a new antibody detection format (QuickELISA). With one single incubation step which involves all reactants except the enzyme substrate, the QuickELISA is particularly suited for automation. We formatted the QuickELISA for the Triturus EIA analyzer for testing large numbers of samples. We found that in QuickELISA formats rGP50 and rT24H have better sensitivity and specificity than sTs18var1 for detecting porcine cysticercosis.
Handali
Sukwan
S
Atlanta Research and Education Foundation, Atlanta, Georgia, USA. ahi0@cdc.gov
Pattabhi
Sowmya
S
Lee
Yeuk-Mui
YM
Silva-Ibanez
Maria
M
Kovalenko
Victor A
VA
Levin
Andrew E
AE
Gonzalez
Armando E
AE
Roberts
Jacquelin M
JM
Garcia
Hector H
HH
Gilman
Robert H
RH
Hancock
Kathy
K
Tsang
Victor C W
VC
D000818:Animals; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*