Assessment of cell-free DNA with microvascular complication of type II diabetes mellitus, using PCR and ELISA.
In order to assess the potential biochemical markers in the development, diagnosis, and prognosis of diabetic patient with microvascular complication represented with retinopathy, we analyzed the levels of cell-free DNA by two different techniques. The levels of cell-free GAPDH assayed by quantitative PCR were significantly higher in the plasma samples of diabetic patients with and without diabetic retinopathy than in those of the control group; thus, it is a better biomarker than nucleosomes assayed by ELISA in patients with type 2 diabetes for the early detection of development of microvascular complications as retinopathy.
El Tarhouny
Shereen A
SA
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. shereeneltarhony@hotmail.com
Hadhoud
Khaled M
KM
Ebrahem
Magdy M
MM
Al Azizi
Nashwa M
NM
D000368:Aged; D002477:Cells; D004247:DNA / Q000097:blood / Q000235:genetics*; D048909:Diabetes Complications / Q000097:blood / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009707:Nucleosomes / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D009707:Nucleosomes; D004247:DNA