Analysis of Recombinant CD24 Glycans by MALDI-TOF-MS Reveals Prevalence of Sialyl-T Antigen.
CD24 is a glycosyl-phosphatidyl-inositol linked glycoprotein expressed in a broad range of cell types and is heavily glycosylated. It has been found to be over expressed in cancers and tumors and is also a costimulatory molecule. Therefore, this study was carried out to define the structures of the carbohydrates associated with the CD24 recombinant protein. The CD24 glycoprotein's oligosaccharides were released by chemical and enzymatic means prior to being analyzed by MALDI-TOF-MS. The results obtained showed that CD24 is both N- and O-glycosylated. The major oligosaccharides were found to be Neu5Acα-2,3/6Galβ-1,3GalNAc, NeuAc(2)Gal β-1,3GalNAc(1) (O-glycans), GalNAc(2)GlcNAc(2)Man(3)Fuc(1), Gal(1)GalNAc(2)GlcNAc(2)Man(3)Fuc(1), and Gal(2)GalNAc(2)GlcNAc(2)Man(3)Fuc(1) (N-glycans). The results showed that Neu5Acα-2,3/6Galβ-1,3GalNAc (sialyl-tumor antigen, sT), a cancer-associated carbohydrate, was the most abundant glycan associated with CD24. This result raised the intriguing possibility that CD24 may be a major carrier of the sialyl-T abundantly found in cancer cells.
Motari
Edwin
E
Departments of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Zheng
Xincheng
X
Su
Xiaodan
X
Liu
Yang
Y
Kvaratskhelia
Mamuka
M
Freitas
Michael
M
Wang
Peng George
PG
J Clin Pathol. 1998 Aug;51(8):588-92
9828816
Science. 2002 Oct 25;298(5594):850-4
12242449
Histopathology. 1994 Dec;25(6):537-43
7698730
Cancer. 1993 Apr 1;71(7):2191-9
8384065
Cancer Res. 1992 Oct 1;52(19):5264-70
1327504
J Immunol. 1991 Aug 15;147(4):1412-6
1831224
J Neurosci. 1996 Apr 15;16(8):2624-34
8786438
Glycoconj J. 1998 Aug;15(8):737-47
9870349
J Exp Med. 1994 Apr 1;179(4):1391-5
8145052
Pathol Res Pract. 2005;201(7):479-86
16164042
APMIS Suppl. 1999;95:1-39
10599242
Acta Derm Venereol. 1998 May;78(3):173-6
9602220
APMIS. 1993 Mar;101(3):242-8
8389564
J Exp Med. 1992 Feb 1;175(2):437-45
1346270
Blood. 1997 May 1;89(9):3385-95
9129046
Cancer Res. 1989 Jan 1;49(1):197-204
2908846
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):15041-6
14657362
Anal Biochem. 1997 Oct 1;252(1):40-7
9324939
J Histochem Cytochem. 1994 Sep;42(9):1251-9
8064132
Eur J Immunol. 1992 Nov;22(11):2855-9
1385153
Biochem Biophys Res Commun. 1996 Aug 14;225(2):384-91
8753773
Am J Pathol. 2002 Oct;161(4):1215-21
12368195
J Exp Med. 2004 Oct 18;200(8):1083-9
15477346
J Biol Chem. 1995 Mar 17;270(11):5979-84
7534304
Biochem J. 1999 Mar 1;338 ( Pt 2):529-38
10024532
Kidney Int. 1997 May;51(5):1418-26
9150453
J Exp Med. 2006 Jul 10;203(7):1713-20
16769998
Blood. 1996 Oct 15;88(8):3048-55
8874203
Anticancer Res. 1994 Mar-Apr;14(2B):609-15
7516636
Anal Biochem. 1997 Feb 1;245(1):17-27
9025964
Clin Cancer Res. 2003 Oct 15;9(13):4906-13
14581365
Curr Opin Struct Biol. 1996 Oct;6(5):679-91
8913692
FASEB J. 1998 Sep;12(12):1241-51
9737727
Virchows Arch. 1995;427(3):251-8
7496593
Prostate. 2004 Feb 1;58(2):183-92
14716744
Hum Pathol. 1994 Apr;25(4):364-72
8163269
Oral Oncol. 1998 Jan;34(1):44-51
9659519
APMIS Suppl. 1992;27:162-72
1520525
Cell. 2004 Apr 16;117(2):265-77
15084263
Int Immunol. 1995 Oct;7(10):1557-65
8562500
J Immunol. 1990 Sep 15;145(6):1952-9
2118158
J Exp Med. 2004 Aug 16;200(4):447-58
15314074
Virchows Arch. 2000 Aug;437(2):173-9
10993278
Anal Chem. 2001 Dec 15;73(24):6063-9
11791581
Virchows Arch. 1994;425(4):399-406
7820302
Rapid Commun Mass Spectrom. 2005;19(23):3421-8
16252310
Biochimie. 2003 Jun;85(6):565-73
12829373
Biochim Biophys Acta. 1997 Feb 8;1337(2):287-94
9048906
Cancer. 1993 Aug 15;72(4):1147-54
8393363