title: Active archaeal communities at cold seep sediments populated by Siboglinidae tubeworms from the Storegga Slide.
abstract: Siboglinid tubeworms in cold seep sediments can locally modify the geochemical gradients of electron acceptors and donors, hence creating potential microhabitats for prokaryotic populations. The archaeal communities associated with sediments populated by Oligobrachia haakonmosbiensis and Sclerolinum contortum Siboglinid tubeworms in the Storegga Slide were examined in this study. Vertical distribution of archaeal communities was investigated using denaturing gradient gel electrophoresis based on 16S rRNA genes. The active fraction of the archaeal community was assessed by using reverse-transcribed rRNA. Archaeal communities associated with sediments colonized by tubeworms were affiliated with uncultivated archaeal lineages of the Crenarchaeota and Euryarchaeota. The composition of the active archaeal populations changed with depth indicating a reorganization of microbial communities. 16S rRNA gene libraries were dominated by sequences affiliated to the Rice Cluster V which are unusual in marine sediment samples. Moreover, this study provides the first evidence of living Crenarchaeota of the Rice Cluster V in cold seep sediments. Furthermore, the Storegga Slide sediments harbored a high diversity of other minor groups of uncultivated lineages including Terrestrial Miscellaneous Euryarchaeotal Group, Marine Benthic Group (MBG)-D, MBG-E, Deep-Sea Hydrothermal Vent Euryarchaeotal Group, Lake Dagow Sediment, Val Kotinen Lake clade III, and Sippenauer Moor 1. Thus, we hypothesize that the vertical geochemical imprint created by the tubeworms could support broad active archaeal populations in the Siboglinidae-populated Storegga Slide sediments.
authors.affiliation: Laboratoire de Microbiologie des Environnements Extrêmes, UMR 6197, Ifremer Centre de Brest, Département Etudes des Environnements Profonds, Université de Bretagne Occidentale, BP 70, 29280 Plouzané, France.
authors.forename: Cassandre Sara
authors.initials: CS
authors.lastname: Lazar
authors.forename: Julie
authors.initials: J
authors.lastname: Dinasquet
authors.forename: Patricia
authors.initials: P
authors.lastname: Pignet
authors.forename: Daniel
authors.initials: D
authors.lastname: Prieur
authors.forename: Laurent
authors.initials: L
authors.lastname: Toffin
mesh_terms: D000818:Animals; D019615:Crenarchaeota / Q000145:classification / Q000235:genetics*; D016172:DNA Fingerprinting; D019641:DNA, Archaeal / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D017753:Ecosystem; D015723:Gene Library; D019015:Geologic Sediments / Q000382:microbiology*; D009664:Norway; D010802:Phylogeny; D011077:Polychaeta / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S
references.citation: Nature. 2000 Oct 5;407(6804):623-6
references.pmid: 11034209
references.citation: J Microbiol Methods. 2003 Oct;55(1):155-64
references.pmid: 14500007
references.citation: Appl Environ Microbiol. 2000 Feb;66(2):499-508
references.pmid: 10653710
references.citation: Environ Microbiol. 2008 Dec;10(12):3237-54
references.pmid: 18707616
references.citation: Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2815-20
references.pmid: 16477011
references.citation: Nature. 2005 Sep 22;437(7058):543-6
references.pmid: 16177789
references.citation: Microb Ecol. 2004 Oct;48(3):389-99
references.pmid: 15692859
references.citation: FEMS Microbiol Ecol. 2004 Oct 1;50(1):1-11
references.pmid: 19712372
references.citation: Appl Environ Microbiol. 1997 Sep;63(9):3367-73
references.pmid: 9292986
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2010 Jan;155(1):41-8
references.pmid: 19770067
references.citation: J Mol Biol. 1990 Oct 5;215(3):403-10
references.pmid: 2231712
references.citation: FEMS Microbiol Ecol. 2006 Oct;58(1):86-98
references.pmid: 16958910
references.citation: PLoS Biol. 2005 Mar;3(3):e102
references.pmid: 15760275
references.citation: ISME J. 2008 Jan;2(1):3-18
references.pmid: 18180743
references.citation: Appl Environ Microbiol. 2001 Dec;67(12):5750-60
references.pmid: 11722932
references.citation: Nature. 1992 Mar 12;356(6365):148-9
references.pmid: 1545865
references.citation: Appl Environ Microbiol. 2004 Apr;70(4):2404-13
references.pmid: 15066838
references.citation: J Microbiol Methods. 2004 May;57(2):279-81
references.pmid: 15063068
references.citation: Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5685-9
references.pmid: 1608980
references.citation: Appl Environ Microbiol. 2003 Dec;69(12):7224-35
references.pmid: 14660370
references.citation: Appl Environ Microbiol. 1998 Dec;64(12):4983-9
references.pmid: 9835592
references.citation: Appl Environ Microbiol. 2007 May;73(10):3348-62
references.pmid: 17369343
references.citation: Environ Microbiol. 2009 Mar;11(3):687-99
references.pmid: 19207564
references.citation: Can J Microbiol. 2006 Feb;52(2):73-116
references.pmid: 16541146
references.citation: Genetics. 1999 Aug;152(4):1285-97
references.pmid: 10430559
references.citation: Appl Environ Microbiol. 2002 Apr;68(4):1585-94
references.pmid: 11916672
references.citation: Environ Microbiol. 2002 Jun;4(6):338-48
references.pmid: 12071979
references.citation: FEMS Microbiol Ecol. 2000 Oct 1;34(1):45-56
references.pmid: 11053735
references.citation: Nature. 2006 Oct 19;443(7113):854-8
references.pmid: 17051217
references.citation: Appl Environ Microbiol. 1996 Feb;62(2):316-22
references.pmid: 8593035
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: Appl Environ Microbiol. 1999 Oct;65(10):4375-84
references.pmid: 10508063
references.citation: Microb Ecol. 2009 Nov;58(4):819-26
references.pmid: 19562245
references.citation: Mar Biotechnol (NY). 1999 Jul;1(4):391-400
references.pmid: 10489418
references.citation: Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3846-51
references.pmid: 16505362
references.citation: J Microbiol Methods. 2000 Apr;40(2):153-62
references.pmid: 10699671
references.citation: Appl Environ Microbiol. 1996 Feb;62(2):625-30
references.pmid: 8593063
references.citation: Appl Environ Microbiol. 2001 Sep;67(9):4374-6
references.pmid: 11526051
references.citation: Mikrobiologiia. 2000 Nov-Dec;69(6):831-43
references.pmid: 11195584