title: Role of leukaemia inhibitory factor in the induction of pluripotent stem cells in mice.
abstract: iPS (induced pluripotent stem) cells can be induced from somatic cells in mice by genetic manipulation. Most previously established mouse iPS cell lines have been derived using feeder layers supplemented with exogenous LIF (leukaemia inhibitory factor). Although a feeder-free induction system has been developed in recent studies, LIF is still required for reprogramming, but its role in the generation of mouse iPS cells has remained elusive. In this study, we investigated its contribution to the induction of pluripotency. Our results showed that LIF activates AP (alkaline phosphatase) through a c-Myc-dependent mechanism. Moreover, it acts as a protective factor during the transition from AP-positive colonies to Oct3/4-positive cells. These findings illustrate a mechanism by which LIF may integrate signalling into reprogramming.
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Zhouping
authors.initials: Z
authors.lastname: Tang
authors.forename: Yanqiang
authors.initials: Y
authors.lastname: Zhan
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhang
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Yan
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Xiong
authors.forename: Xuewei
authors.initials: X
authors.lastname: Xie
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Suming
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D053605:Leukemia Inhibitory Factor; D016271:Proto-Oncogene Proteins c-myc; D000469:Alkaline Phosphatase