A hierarchy of self-renewing tumor-initiating cell types in glioblastoma.
The neural stem cell marker CD133 is reported to identify cells within glioblastoma (GBM) that can initiate neurosphere growth and tumor formation; however, instances of CD133(-) cells exhibiting similar properties have also been reported. Here, we show that some PTEN-deficient GBM tumors produce a series of CD133(+) and CD133(-) self-renewing tumor-initiating cell types and provide evidence that these cell types constitute a lineage hierarchy. Our results show that the capacities for self-renewal and tumor initiation in GBM need not be restricted to a uniform population of stemlike cells, but can be shared by a lineage of self-renewing cell types expressing a range of markers of forebrain lineage.
Chen
Ruihuan
R
Department of Tumor Biology and Angiogenesis, Genentech Inc., San Francisco, CA 94080, USA.
Nishimura
Merry C
MC
Bumbaca
Stephanie M
SM
Kharbanda
Samir
S
Forrest
William F
WF
Kasman
Ian M
IM
Greve
Joan M
JM
Soriano
Robert H
RH
Gilmour
Laurie L
LL
Rivers
Celina Sanchez
CS
Modrusan
Zora
Z
Nacu
Serban
S
Guerrero
Steve
S
Edgar
Kyle A
KA
Wallin
Jeffrey J
JJ
Lamszus
Katrin
K
Westphal
Manfred
M
Heim
Susanne
S
James
C David
CD
VandenBerg
Scott R
SR
Costello
Joseph F
JF
Moorefield
Scott
S
Cowdrey
Cynthia J
CJ
Prados
Michael
M
Phillips
Heidi S
HS
D000071916:AC133 Antigen; D015703:Antigens, CD / Q000032:analysis / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D022082:Cellular Structures / Q000473:pathology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D008040:Genetic Linkage; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology*; D006023:Glycoproteins / Q000032:analysis / Q000172:deficiency / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D014411:Neoplastic Stem Cells / Q000473:pathology / Q000637:transplantation; D010455:Peptides / Q000032:analysis / Q000172:deficiency / Q000235:genetics
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides