title: Quantum dots decorated with pathogen associated molecular patterns as fluorescent synthetic pathogen models.
abstract: We attached the pathogen associated molecular pattern Kdo(2)-Lipid A (the lipopolysaccharide (LPS) from Escherichia coli (E. coli)) to QDs by hydrophobic interactions to synthetically mimic the surface of E. coli. QD-LPS conjugates bind, are taken up and activate effectively macrophages in vitro and they have potent immunostimulatory activity in vivo.
authors.affiliation: Institute of Immunology & Infection Research, School of Biological Science, University of Edinburgh, Edinburgh, United Kingdom EH9 3JT.
authors.forename: Tom A
authors.initials: TA
authors.lastname: Barr
authors.forename: Martin
authors.initials: M
authors.lastname: Krembuszewski
authors.forename: Manish
authors.initials: M
authors.lastname: Gupta
authors.forename: David
authors.initials: D
authors.lastname: Gray
authors.forename: Juan C
authors.initials: JC
authors.lastname: Mareque-Rivas
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D004926:Escherichia coli / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008050:Lipid A / Q000737:chemistry* / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D045663:Quantum Dots
chemical_list: D000276:Adjuvants, Immunologic; D008050:Lipid A
references.citation: EMBO J. 2006 Feb 22;25(4):683-92
references.pmid: 16467847
references.citation: Nat Protoc. 2007;2(10):2383-90
references.pmid: 17947980
references.citation: Nature. 1991 Jul 25;352(6333):342-4
references.pmid: 1852209
references.citation: Nat Methods. 2008 May;5(5):397-9
references.pmid: 18425138
references.citation: Science. 1992 Oct 23;258(5082):593-7
references.pmid: 1411569
references.citation: J Am Chem Soc. 2005 Aug 24;127(33):11835-41
references.pmid: 16104762
references.citation: Nat Biotechnol. 2004 Feb;22(2):198-203
references.pmid: 14704683
references.citation: Nat Rev Mol Cell Biol. 2002 Dec;3(12):906-18
references.pmid: 12461557
references.citation: Chem Commun (Camb). 2008 Apr 28;(16):1910-2
references.pmid: 18401515
references.citation: Science. 2007 Jun 15;316(5831):1628-32
references.pmid: 17569868
references.citation: Science. 2001 Mar 23;291(5512):2357-64
references.pmid: 11269316
references.citation: Chem Commun (Camb). 2006 Apr 21;(15):1595-7
references.pmid: 16582990
references.citation: Colloids Surf B Biointerfaces. 2007 Sep 1;59(1):1-10
references.pmid: 17544637
references.citation: Nature. 2009 Jul 9;460(7252):264-8
references.pmid: 19525933
references.citation: Angew Chem Int Ed Engl. 2007;46(29):5528-31
references.pmid: 17585399
references.citation: Nat Rev Immunol. 2003 Feb;3(2):169-76
references.pmid: 12563300
references.citation: Science. 2005 Jan 28;307(5709):538-44
references.pmid: 15681376
references.citation: Nat Methods. 2008 Sep;5(9):763-75
references.pmid: 18756197
references.citation: Curr Opin Biotechnol. 2005 Feb;16(1):63-72
references.pmid: 15722017
references.citation: J Immunol Methods. 1998 Mar 15;212(2):187-92
references.pmid: 9672206
references.citation: Nanotechnology. 2005 Feb;16(2):R9-R25
references.pmid: 21727419
references.citation: Nat Immunol. 2008 Apr;9(4):343-5
references.pmid: 18349810
references.citation: Bioconjug Chem. 2008 Oct;19(10):1964-7
references.pmid: 18800819
references.citation: Nat Methods. 2005 Feb;2(2):99-104
references.pmid: 15782206
references.citation: J Immunol. 1998 Nov 15;161(10):5413-20
references.pmid: 9820516
references.citation: Nat Med. 2005 Apr;11(4 Suppl):S63-8
references.pmid: 15812492
references.citation: Infect Immun. 2000 Apr;68(4):1953-63
references.pmid: 10722588
references.citation: Science. 2002 Nov 29;298(5599):1759-62
references.pmid: 12459582
references.citation: Curr Opin Chem Biol. 2000 Dec;4(6):696-703
references.pmid: 11102876
references.citation: Nature. 2002 Dec 19-26;420(6917):885-91
references.pmid: 12490963
references.citation: Nat Biotechnol. 1999 Nov;17(11):1075-81
references.pmid: 10545912
references.citation: Angew Chem Int Ed Engl. 2006 Jul 24;45(30):4936-40
references.pmid: 16807952
references.citation: J Am Chem Soc. 2002 Jun 5;124(22):6256-8
references.pmid: 12033851
references.citation: J Am Chem Soc. 2009 Jun 17;131(23):8102-7
references.pmid: 19462994
references.citation: Angew Chem Int Ed Engl. 1998 Nov 2;37(20):2754-2794
references.pmid: 29711117
references.citation: Mol Syst Biol. 2006;2:2006.0015
references.pmid: 16738560
references.citation: Biophys J. 1997 Mar;72(3):1031-46
references.pmid: 9138554
references.citation: Angew Chem Int Ed Engl. 2009;48(2):356-60
references.pmid: 19053122
references.citation: Nat Mater. 2005 Jun;4(6):435-46
references.pmid: 15928695