title: Identification and typing of Lactococcus lactis by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: Currently, the genus Lactococcus is classified into six species: Lactococcus chungangensis, L. garvieae, L. lactis, L. piscium, L. plantarum, and L. raffinolactis. Among these six species, L. lactis is especially important because of its use in the manufacture of probiotic dairy products. L. lactis consists of three subspecies: L. lactis subsp. cremoris, L. lactis subsp. hordniae, and L. lactis subsp. lactis. However, these subspecies have not yet been reliably discriminated. To date, mainly phenotypic identification has been used, with a few genotypic identifications. We discriminated species or subspecies in the genus Lactococcus not only by proteomics identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) but also by phenotypic and genotypic identification. The proteomics identification using differences in the mass spectra of ribosomal proteins was nearly identical to that by genotypic identification (i.e., by analyses of 16S rRNA and recA gene sequences and amplified fragment length polymorphism). The three ribosomal subunits 30S/L31, 50S/L31, and 50S/L35 were the best markers for discriminating L. lactis subsp. cremoris from L. lactis subsp. lactis. Proteomics identification using MALDI-TOF MS was therefore a powerful method for discriminating and identifying these bacteria. In addition, this method was faster and more reliable than others that we examined.
authors.affiliation: Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186-8650, Japan. kana-tanigawa@yakult.co.jp
authors.forename: Kana
authors.initials: K
authors.lastname: Tanigawa
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kawabata
authors.forename: Koichi
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D005838:Genotype; D013294:Lactococcus lactis / Q000737:chemistry* / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D012269:Ribosomal Proteins / Q000032:analysis* / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D012269:Ribosomal Proteins; D011938:Rec A Recombinases
references.citation: Int J Syst Evol Microbiol. 2007 Dec;57(Pt 12):2777-2789
references.pmid: 18048724
references.citation: Appl Environ Microbiol. 1999 Nov;65(11):5151-3
references.pmid: 10543836
references.citation: Appl Environ Microbiol. 1991 May;57(5):1313-8
references.pmid: 1713027
references.citation: Appl Environ Microbiol. 2007 Oct;73(20):6601-11
references.pmid: 17704267
references.citation: FEMS Microbiol Lett. 1998 Sep 1;166(1):15-20
references.pmid: 9741080
references.citation: Appl Environ Microbiol. 2008 Sep;74(17):5402-7
references.pmid: 18606788
references.citation: Anal Chem. 2003 Aug 1;75(15):3817-22
references.pmid: 14572048
references.citation: Appl Environ Microbiol. 2002 May;68(5):2209-13
references.pmid: 11976090
references.citation: J Clin Microbiol. 2008 Jun;46(6):1946-54
references.pmid: 18400920
references.citation: Appl Environ Microbiol. 1993 Nov;59(11):3941-5
references.pmid: 7506898
references.citation: Appl Environ Microbiol. 1992 Dec;58(12):4045-7
references.pmid: 16348830
references.citation: Genome Res. 2001 May;11(5):731-53
references.pmid: 11337471
references.citation: Microbiology (Reading). 2002 Nov;148(Pt 11):3332-3333
references.pmid: 12427924
references.citation: J Clin Microbiol. 2007 Aug;45(8):2392-7
references.pmid: 17553974
references.citation: J Appl Microbiol. 2006 Aug;101(2):396-405
references.pmid: 16882147
references.citation: Appl Environ Microbiol. 1997 Feb;63(2):694-702
references.pmid: 9023947
references.citation: J Med Microbiol. 2007 Jan;56(Pt 1):102-109
references.pmid: 17172524
references.citation: Int J Syst Evol Microbiol. 2001 Nov;51(Pt 6):2113-2117
references.pmid: 11760954
references.citation: J Bacteriol. 1983 Apr;154(1):1-9
references.pmid: 6403500
references.citation: Proteomics. 2006 Dec;6(24):6416-25
references.pmid: 17109381
references.citation: Nucleic Acids Res. 1993 Nov 11;21(22):5279-80
references.pmid: 8255788
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Science. 2000 Aug 25;289(5483):1352-5
references.pmid: 10958782
references.citation: Appl Environ Microbiol. 2005 Oct;71(10):6292-307
references.pmid: 16204551
references.citation: Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801-5
references.pmid: 368799
references.citation: J Proteome Res. 2007 Oct;6(10):3899-907
references.pmid: 17854216
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: Appl Environ Microbiol. 2008 Dec;74(24):7767-78
references.pmid: 18952875
references.citation: Appl Environ Microbiol. 1981 Jan;41(1):77-83
references.pmid: 16345703
references.citation: Int J Syst Bacteriol. 1999 Jan;49 Pt 1:163-6
references.pmid: 10028257
references.citation: J Appl Microbiol. 2002;93(2):353-61
references.pmid: 12147085
references.citation: J Bacteriol. 2007 Apr;189(8):3256-70
references.pmid: 17307855
references.citation: Microbiology (Reading). 1994 Jun;140 ( Pt 6):1301-5
references.pmid: 8081494
references.citation: Appl Environ Microbiol. 2007 Nov;73(22):7128-37
references.pmid: 17890345
references.citation: Proc Natl Acad Sci U S A. 2006 Oct 17;103(42):15611-6
references.pmid: 17030793