title: The RING finger domain of Varicella-Zoster virus ORF61p has E3 ubiquitin ligase activity that is essential for efficient autoubiquitination and dispersion of Sp100-containing nuclear bodies.
abstract: Varicella zoster virus encodes an immediate-early (IE) protein termed ORF61p that is orthologous to the herpes simplex virus IE protein ICP0. Although these proteins share several functional properties, ORF61p does not fully substitute for ICP0. The greatest region of similarity between these proteins is a RING finger domain. We demonstrate that disruption of the ORF61p RING finger domain by amino acid substitution (Cys19Gly) alters ORF61p intranuclear distribution and abolishes ORF61p-mediated dispersion of Sp100-containing nuclear bodies. In addition, we demonstrate that an intact ORF61p RING finger domain is necessary for E3 ubiquitin ligase activity and is required for autoubiquitination and regulation of protein stability.
authors.affiliation: Department of Microbiology and Immunology, College of Physicians and Surgeons, Columbia University, 701 W. 168th St., New York, NY 10032, USA.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Walters
authors.forename: Christos A
authors.initials: CA
authors.lastname: Kyratsous
authors.forename: Saul J
authors.initials: SJ
authors.lastname: Silverstein
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D034961:Antigens, Nuclear / Q000378:metabolism*; D001324:Autoantigens / Q000378:metabolism*; D002467:Cell Nucleus / Q000737:chemistry; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000502:physiology*; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D055550:Protein Stability; D054829:RING Finger Domains; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D034961:Antigens, Nuclear; D001324:Autoantigens; D017874:Immediate-Early Proteins; D014764:Viral Proteins; C073411:protein 61, Varicella-zoster virus; C066642:SP100 protein, human; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1
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