title: [Establishment and characterization of a human gallbladder carcinoma cell line EH-GB1 originated from a metastatic tumor].
abstract: OBJECTIVE
  To establish a human gallbladder carcinoma cell line derived from a metastatic gallbladder carcinoma and identify its biological characteristics.
  
  
  METHODS
  Tissue samples were separated from the surgical specimen obtained from a patient with metastatic carcinoma and single-cell suspension was prepared. Then the cells were cultured in DMEM medium supplemented with 15% fetal bovine serum. The morphology of tumor cells was observed under an electron microscope. The cell growth curve was plotted. The tumorigenicity of the cell line was studied by subcutaneous inoculation in SCID mice. The cells were infected by lentiviral vector carrying fluorescent report genes (lenti-GFP and lenti-Red2) separately for expressions of GFP and Red2, respectively.
  
  
  RESULTS
  A novel metastatic gallbladder carcinoma cell line was successfully established and named "EH-GB1". It could be passaged for over 20 generations with typical malignant epithelial morphology and a stable growth cycle of 24 h. Tumors were formed in all of the 10 SCID mice inoculated with EH-GB1 cells subcutaneously, and the tumor cells were tumor marker CA19-9-positive. Continuous expressions of fluorescent report genes were observed in EH-GB1 cells infected by lenti-GFP and lenti-Red2.
  
  
  CONCLUSIONS
  EH-GB1 cells might be the first stable cell line of human gallbladder carcinoma established from a metastatic focus of gallbladder carcinoma. This cell line with continuous expressions of GFP and Red2 might be a novel and perfect experimental model for clinical and basic research on gallbladder carcinoma.
authors.affiliation: Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Lin-fang
authors.initials: LF
authors.lastname: Li
authors.forename: Huan-zhang
authors.initials: HZ
authors.lastname: Hu
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.forename: Jing-han
authors.initials: JH
authors.lastname: Wang
authors.forename: Hong-ping
authors.initials: HP
authors.lastname: Wu
authors.forename: Hua-jun
authors.initials: HJ
authors.lastname: Jin
authors.forename: Chang-qing
authors.initials: CQ
authors.lastname: Su
authors.forename: Xiao-qing
authors.initials: XQ
authors.lastname: Jiang
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Gu
authors.forename: Meng-chao
authors.initials: MC
authors.lastname: Wu
authors.forename: Qi-jun
authors.initials: QJ
authors.lastname: Qian
mesh_terms: D000008:Abdominal Neoplasms / Q000378:metabolism / Q000473:pathology / Q000556:secondary*; D034861:Abdominal Wall; D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology* / Q000556:secondary; D000818:Animals; D018395:CA-19-9 Antigen / Q000378:metabolism; D045744:Cell Line, Tumor / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005706:Gallbladder Neoplasms / Q000378:metabolism / Q000473:pathology*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D008875:Middle Aged; D009368:Neoplasm Transplantation
chemical_list: D018395:CA-19-9 Antigen; D049452:Green Fluorescent Proteins