When is weaker better? Design of an ADP sensor with weak ADP affinity, but still selective against ATP.
A simple ADP biosensor would be of broad usefulness in monitoring the large number of metabolic processes that produce ADP. Several new systems have been recently described including one in the current issue of ACS Chemical Biology that provides a simple readout of the ADP concentration without significant interference by ATP.
Hackney
David D
DD
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA. ddh@andrew.cmu.edu
D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000032:analysis* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D008958:Models, Molecular; D012680:Sensitivity and Specificity
D000199:Actins; D029968:Escherichia coli Proteins; C471255:ParM protein, E coli; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate