[Determination of the contents of chlorogenic acid and phillyrin of Shuanghuanglian oral fluid using NIRS].
The aim of the present study was to establish the model of predicting the contents of chlorogenic acid and phillyrin in Shuanghuanglian oral fluid using NIR to realize quick quality evaluation of Shuanghuanglian oral fluid. To this end, many batches of Shuanghuanglian oral fluid were selected, and the contents of chlorogenic acid and phillyrin were determined using HPLC. Meanwhile, the NIR spectra of the same samples were determined. The model used to predict the contents of chlorogenic acid and phillyrin in Shuanghuanglian oral fluid was established by correlation analysis between the results gained by HPLC and NIR spectra. According to the value of RSEP and r, the method of data processing was chosen. The method of spectra processing and wavelength range or wave numbers were chosen based on the value of RMSECV. The method of data processing was SMLR The original spectra were used to establish the model. The wave numbers in the model used to predict the contents of chlorogenic acid and phillyrin were 6 654.06/7 106.08 cm(-1), and 5 456.06/7 222.08 cm(-1) respectively. The RMSECV and the correlation coefficient of the best model of chlorogenic acid and phillyrin were 0.857 26, 0.889 87 and 0.857 26 and 0.889 87. The results of cross validation indicate that the predicting model was accurate and credible, and could be used as a rapid quality control method of Shuanghuanglian oral fluid.
Dai
Chuan-Yun
CY
Department of Biology, Chongqing University of Science and Technology, Chongqing 401331, China. cydai@126.com
Gao
Xiao-Yan
XY
Tang
Bo
B
Fu
Ya
Y
Liu
Huo-An
HA
D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D011786:Quality Control
D004365:Drugs, Chinese Herbal; D005960:Glucosides; C516400:shuang-huang-lian; D002726:Chlorogenic Acid; C075528:phillyrin