Endocytic pathway is required for Drosophila Toll innate immune signaling.
The Toll signaling pathway is required for the innate immune response against fungi and Gram-positive bacteria in Drosophila. Here we show that the endosomal proteins Myopic (Mop) and Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) are required for the activation of the Toll signaling pathway. This requirement is observed in cultured cells and in flies, and epistasis experiments show that the Mop protein functions upstream of the MyD88 adaptor and the Pelle kinase. Mop and Hrs, which are critical components of the ESCRT-0 endocytosis complex, colocalize with the Toll receptor in endosomes. We conclude that endocytosis is required for the activation of the Toll signaling pathway.
Huang
Hon-Ren
HR
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Chen
Zhijian J
ZJ
Kunes
Sam
S
Chang
Geen-Dong
GD
Maniatis
Tom
T
D000818:Animals; D002460:Cell Line; D029721:Drosophila Proteins / Q000235:genetics / Q000276:immunology*; D004331:Drosophila melanogaster / Q000276:immunology* / Q000378:metabolism; D004705:Endocytosis; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000276:immunology*; D007113:Immunity, Innate; D010750:Phosphoproteins / Q000235:genetics / Q000276:immunology*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000276:immunology*; D034622:RNA Interference; D015398:Signal Transduction; D051193:Toll-Like Receptors / Q000276:immunology* / Q000378:metabolism
D029721:Drosophila Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D010750:Phosphoproteins; D051193:Toll-Like Receptors; C096164:hepatocyte growth factor-regulated tyrosine kinase substrate; C549822:Mop protein, Drosophila; D017027:Protein Tyrosine Phosphatases
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