title: AlphaE-catenin regulates actin dynamics independently of cadherin-mediated cell-cell adhesion.
abstract: alphaE-catenin binds the cell-cell adhesion complex of E-cadherin and beta-catenin (beta-cat) and regulates filamentous actin (F-actin) dynamics. In vitro, binding of alphaE-catenin to the E-cadherin-beta-cat complex lowers alphaE-catenin affinity for F-actin, and alphaE-catenin alone can bind F-actin and inhibit Arp2/3 complex-mediated actin polymerization. In cells, to test whether alphaE-catenin regulates actin dynamics independently of the cadherin complex, the cytosolic alphaE-catenin pool was sequestered to mitochondria without affecting overall levels of alphaE-catenin or the cadherin-catenin complex. Sequestering cytosolic alphaE-catenin to mitochondria alters lamellipodia architecture and increases membrane dynamics and cell migration without affecting cell-cell adhesion. In contrast, sequestration of cytosolic alphaE-catenin to the plasma membrane reduces membrane dynamics. These results demonstrate that the cytosolic pool of alphaE-catenin regulates actin dynamics independently of cell-cell adhesion.
authors.affiliation: Cancer Biology Program, Stanford University, Stanford, CA 94305, USA.
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Benjamin
authors.forename: Adam V
authors.initials: AV
authors.lastname: Kwiatkowski
authors.forename: Changsong
authors.initials: C
authors.lastname: Yang
authors.forename: Farida
authors.initials: F
authors.lastname: Korobova
authors.forename: Sabine
authors.initials: S
authors.lastname: Pokutta
authors.forename: Tatyana
authors.initials: T
authors.lastname: Svitkina
authors.forename: William I
authors.initials: WI
authors.lastname: Weis
authors.forename: W James
authors.initials: WJ
authors.lastname: Nelson
mesh_terms: D051376:Actin-Related Protein 2-3 Complex / Q000235:genetics / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002465:Cell Movement / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism; D004285:Dogs; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D055503:Protein Multimerization; D011554:Pseudopodia / Q000378:metabolism; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D051178:alpha Catenin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D015820:Cadherins; D011993:Recombinant Fusion Proteins; D051178:alpha Catenin; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases
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