A chemical screen to identify novel inhibitors of fin regeneration in zebrafish.
We performed a chemical screen to look for novel inhibitors of zebrafish caudal fin regeneration. In a pilot screen, 520 compounds were tested. Two compounds, budesonide and AGN192403, abrogated fin regeneration. One compound in particular, AGN192403, targets the imidazoline receptor, a pathway not previously linked to fin regeneration. In addition to inhibiting regeneration of the adult fin, AGN192403 also blocked regeneration of the larval fin fold. Finally, the inhibitory effect of AGN192403 on fin regeneration persisted after removal of the drug. These studies demonstrate that chemical screening is feasible in adult zebrafish and that it is a reasonable strategy to use for exploring the biology of regeneration.
Oppedal
Douglas
D
Department of Pediatrics, Washington University, St. Louis, Missouri 63110, USA.
Goldsmith
Matthew I
MI
D000818:Animals; D019819:Budesonide / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D012038:Regeneration / Q000187:drug effects*; D015027:Zebrafish / Q000033:anatomy & histology / Q000502:physiology*
D019819:Budesonide
Genetics. 1995 Dec;141(4):1583-95
8601496
Naunyn Schmiedebergs Arch Pharmacol. 1984 Feb;325(2):136-44
6144048
J Pharmacol Exp Ther. 1984 Jul;230(1):232-6
6146707
Science. 2005 Dec 23;310(5756):1957-60
16373575
Dev Dyn. 2004 Dec;231(4):693-9
15499559
Br J Pharmacol. 1983 Sep;80(1):155-61
6140052
Chem Biol. 2009 Apr 24;16(4):432-41
19389629
Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14599-604
16204379
J Comp Neurol. 1997 Jan 27;377(4):577-95
9007194
Dev Biol. 2003 Jul 15;259(2):303-17
12871703
Dev Biol. 1997 Dec 15;192(2):289-99
9441668
Dev Biol. 2000 Jun 15;222(2):347-58
10837124
Ann N Y Acad Sci. 1995 Jul 12;763:295-313
7677340
Nat Chem Biol. 2009 Sep;5(9):680-7
19578332
Nat Protoc. 2009;4(10):1422-32
19745824
Development. 2002 Jun;129(11):2607-17
12015289
Genetics. 2000 Jul;155(3):1321-9
10880491
Eur J Pharmacol. 1987 Jan 28;134(1):1-13
3030779
ANZ J Surg. 2007 Jan-Feb;77(1-2):9-14
17295811
J Pharmacol. 1986 Apr-Jun;17(2):113-8
2875218
Science. 2005 Dec 23;310(5756):1919-23
16373567
Development. 2005 Dec;132(23):5173-83
16251209
Development. 2007 Feb;134(3):479-89
17185322
Dev Biol. 2003 Jun 15;258(2):291-306
12798289
Development. 2002 Nov;129(22):5141-9
12399306
Chem Biol Drug Des. 2006 Oct;68(4):213-9
17105485
Biol Reprod. 2001 Feb;64(2):548-54
11159357
Dev Biol. 2009 Jan 1;325(1):71-81
18950614
J Comp Neurol. 1996 Dec 09;376(2):253-64
8951641
Nat Genet. 2000 Oct;26(2):216-20
11017081
Dev Dyn. 2004 Nov;231(3):527-41
15376328
Dev Biol. 2009 Jul 15;331(2):270-80
19445916
Trends Pharmacol Sci. 1992 Oct;13(10):369-70
1413085
Adv Wound Care. 1998 Oct;11(6):277-85
10326344