title: Food deprivation alters thermoregulatory responses to lipopolysaccharide by enhancing cryogenic inflammatory signaling via prostaglandin D2.
abstract: We tested the hypothesis that food deprivation alters body temperature (T(b)) responses to bacterial LPS by enhancing inflammatory signaling that decreases T(b) (cryogenic signaling) rather than by suppressing inflammatory signaling that increases T(b) (febrigenic signaling). Free-feeding or food-deprived (24 h) rats received LPS at doses (500 and 2,500 microg/kg iv) that are high enough to activate both febrigenic and cryogenic signaling. At these doses, LPS caused fever in rats at an ambient temperature of 30 degrees C, but produced hypothermia at an ambient temperature of 22 degrees C. Whereas food deprivation had little effect on LPS fever, it enhanced LPS hypothermia, an effect that was particularly pronounced in rats injected with the higher LPS dose. Enhancement of hypothermia was not due to thermogenic incapacity, since food-deprived rats were fully capable of raising T(b) in response to the thermogenic drug CL316,243 (1 mg/kg iv). Neither was enhancement of hypothermia associated with altered plasma levels of cytokines (TNF-alpha, IL-1beta, and IL-6) or with reduced levels of an anti-inflammatory hormone (corticosterone). The levels of PGD(2) and PGE(2) during LPS hypothermia were augmented by food deprivation, although the ratio between them remained unchanged. Food deprivation, however, selectively enhanced the responsiveness of rats to the cryogenic action of PGD(2) (100 ng icv) without altering the responsiveness to febrigenic PGE(2) (100 ng icv). These findings support our hypothesis and indicate that cryogenic signaling via PGD(2) underlies enhancement of LPS hypothermia by food deprivation.
authors.affiliation: Department of Pharmaceutical Sciences, Albany College of Pharmacy & Health Sciences, Albany, NY 12208, USA.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Krall
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Yao
authors.forename: Martha A
authors.initials: MA
authors.lastname: Hass
authors.forename: Carlos
authors.initials: C
authors.lastname: Feleder
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Steiner
mesh_terms: D000818:Animals; D001831:Body Temperature / Q000187:drug effects* / Q000502:physiology; D003080:Cold Temperature; D005508:Food Deprivation / Q000502:physiology*; D007249:Inflammation; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D015230:Prostaglandin D2 / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology
chemical_list: D008070:Lipopolysaccharides; D015230:Prostaglandin D2
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