title: A hierarchy of self-renewing tumor-initiating cell types in glioblastoma.
abstract: The neural stem cell marker CD133 is reported to identify cells within glioblastoma (GBM) that can initiate neurosphere growth and tumor formation; however, instances of CD133(-) cells exhibiting similar properties have also been reported. Here, we show that some PTEN-deficient GBM tumors produce a series of CD133(+) and CD133(-) self-renewing tumor-initiating cell types and provide evidence that these cell types constitute a lineage hierarchy. Our results show that the capacities for self-renewal and tumor initiation in GBM need not be restricted to a uniform population of stemlike cells, but can be shared by a lineage of self-renewing cell types expressing a range of markers of forebrain lineage.
authors.affiliation: Department of Tumor Biology and Angiogenesis, Genentech Inc., San Francisco, CA 94080, USA.
authors.forename: Ruihuan
authors.initials: R
authors.lastname: Chen
authors.forename: Merry C
authors.initials: MC
authors.lastname: Nishimura
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Bumbaca
authors.forename: Samir
authors.initials: S
authors.lastname: Kharbanda
authors.forename: William F
authors.initials: WF
authors.lastname: Forrest
authors.forename: Ian M
authors.initials: IM
authors.lastname: Kasman
authors.forename: Joan M
authors.initials: JM
authors.lastname: Greve
authors.forename: Robert H
authors.initials: RH
authors.lastname: Soriano
authors.forename: Laurie L
authors.initials: LL
authors.lastname: Gilmour
authors.forename: Celina Sanchez
authors.initials: CS
authors.lastname: Rivers
authors.forename: Zora
authors.initials: Z
authors.lastname: Modrusan
authors.forename: Serban
authors.initials: S
authors.lastname: Nacu
authors.forename: Steve
authors.initials: S
authors.lastname: Guerrero
authors.forename: Kyle A
authors.initials: KA
authors.lastname: Edgar
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Wallin
authors.forename: Katrin
authors.initials: K
authors.lastname: Lamszus
authors.forename: Manfred
authors.initials: M
authors.lastname: Westphal
authors.forename: Susanne
authors.initials: S
authors.lastname: Heim
authors.forename: C David
authors.initials: CD
authors.lastname: James
authors.forename: Scott R
authors.initials: SR
authors.lastname: VandenBerg
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Costello
authors.forename: Scott
authors.initials: S
authors.lastname: Moorefield
authors.forename: Cynthia J
authors.initials: CJ
authors.lastname: Cowdrey
authors.forename: Michael
authors.initials: M
authors.lastname: Prados
authors.forename: Heidi S
authors.initials: HS
authors.lastname: Phillips
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000032:analysis / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D022082:Cellular Structures / Q000473:pathology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D008040:Genetic Linkage; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology*; D006023:Glycoproteins / Q000032:analysis / Q000172:deficiency / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D014411:Neoplastic Stem Cells / Q000473:pathology / Q000637:transplantation; D010455:Peptides / Q000032:analysis / Q000172:deficiency / Q000235:genetics
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides