Meal test for glucose-dependent insulinotropic peptide (GIP) in obese and type 2 diabetic patients.
Glucose-dependent insulinotropic peptide (GIP) contributes to incretin effect of insulin secretion which is impaired in Type 2 diabetes mellitus. The aim of this study was to introduce a simple meal test for evaluation of GIP secretion and action and to examine GIP changes in Type 2 diabetic patients. Seventeen Type 2 diabetic patients, 10 obese non-diabetic and 17 non-obese control persons have been examined before and after 30, 60 and 90 min stimulation by meal test. Serum concentrations of insulin, C-peptide and GIP were estimated during the test. Impaired GIP secretion was found in Type 2 diabetic patients as compared with obese non-diabetic and non-obese control persons. The AUCGIP during 90 min of the meal stimulation was significantly lower in diabetic patients than in other two groups (p<0.03). Insulin concentration at 30 min was lower in diabetic than in non-diabetic persons and the GIP action was delayed. The deltaIRI/deltaGIP ratio increased during the test in diabetic patients, whereas it progressively decreased in obese and non-obese control persons. Simple meal test could demonstrate impaired GIP secretion and delayed insulin secretion in Type 2 diabetic patients as compared to obese non-diabetic and non-obese healthy control individuals.
Skrha
J
J
Third Department of Internal Medicine, First Faculty of Medicine, Charles University, Prague, Czech Republic. jan.skrha@lf1.cuni.cz
Hilgertová
J
J
Jarolímková
M
M
Kunešová
M
M
Hill
M
M
D000328:Adult; D000368:Aged; D002096:C-Peptide / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis* / Q000378:metabolism*; D003940:Diagnostic Techniques, Endocrine; D004041:Dietary Fats / Q000494:pharmacology; D004044:Dietary Proteins / Q000494:pharmacology; D019422:Dietary Sucrose / Q000494:pharmacology; D005749:Gastric Inhibitory Polypeptide / Q000097:blood / Q000378:metabolism*; D005951:Glucose Tolerance Test; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D006943:Hyperglycemia / Q000175:diagnosis / Q000378:metabolism; D007328:Insulin / Q000097:blood; D008875:Middle Aged; D009765:Obesity / Q000175:diagnosis* / Q000378:metabolism*
D002096:C-Peptide; D004041:Dietary Fats; D004044:Dietary Proteins; D019422:Dietary Sucrose; D005749:Gastric Inhibitory Polypeptide; D006442:Glycated Hemoglobin; D007328:Insulin; C517652:hemoglobin A1c protein, human