title: Rational design for over-production of desirable microbial metabolites by precision engineering.
abstract: Microbes represent a valuable source of commercially significant natural products that have improved our quality of life. Precision engineering can be used to precisely identify and specifically modify genes responsible for production of natural products and improve this production or modify the genes creating products that would not otherwise be produced. There have been several success stories concerning the manipulation of regulatory genes, pathways, and genomes to increase the productivity of industrial microbes. This review will focus on the strategies and integrated approaches for precisely deciphering regulatory genes by various modern techniques. The applications of precision engineering in rational strain improvement also shed light on the biology of natural microbial systems.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.forename: Xianlong
authors.initials: X
authors.lastname: Zhou
authors.forename: Zhongxuan
authors.initials: Z
authors.lastname: Gou
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.forename: Chengzhang
authors.initials: C
authors.lastname: Fu
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Fuhang
authors.initials: F
authors.lastname: Song
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ashforth
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D005658:Fungi / Q000235:genetics* / Q000378:metabolism*; D005818:Genetic Engineering