A strong inorganic acid-initiated methacrylate polymerization strategy for room temperature preparation of monolithic columns for capillary electrochromatography.
A facile strong inorganic acid-initiated methacrylate polymerization strategy was developed for fabricating monolithic columns at room temperature. The prepared monoliths were characterized by FTIR spectrometry, mercury intrusion porosimeter and SEM, while their performance was evaluated by CEC for the separation of various types of compounds including alkyl benzenes, polycyclic aromatic hydrocarbons, nonsteroidal anti-inflammatory drugs, anilines, and nitrophenol isomers. The column-to-column and batch-to-batch reproducibility for the prepared monoliths in terms of the RSD of EOF flow velocity, retention factor, and the minimum plate height of naphthalene ranged from 3.4 to 12.4%. The fabricated monoliths gave excellent performance for the separation of the test neutral compounds with the theoretical plates of 170,000-232,000 plates per meter for thiourea, and 77,400-112,300 plates per meter for naphthalene. The proposed strong inorganic acid-initiated methacrylate polymerization strategy is a promising alternative for fabricating organic polymer-based monoliths.
Wang
Man-Man
MM
Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, PR China.
Wang
He-Fang
HF
Jiang
Dong-Qing
DQ
Wang
Shan-Wei
SW
Yan
Xiu-Ping
XP
D053801:Capillary Electrochromatography / Q000379:methods*; D003511:Cyclohexanols / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry; D017942:Nitric Acid / Q000737:chemistry*; D009930:Organic Chemicals; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
D003511:Cyclohexanols; D008689:Methacrylates; D009281:Naphthalenes; D009930:Organic Chemicals; C031721:naphthalene; D017942:Nitric Acid