Methods for the design and analysis of sedimentation velocity and sedimentation equilibrium experiments with proteins.
Analytical ultracentrifugation experiments play an integral role in the solution phase characterization of recombinant proteins and other biological macromolecules. This unit discusses the design of sedimentation velocity and sedimentation equilibrium experiments performed with a Beckman Optima XL-A or XL-I analytical ultracentrifuge. Optimal instrument settings and experimental design considerations are explained, and strategies for the analysis of experimental data with the UltraScan data analysis software package are presented. Special attention is paid to the strengths and weaknesses of the available detectors, and guidance is provided on how to extract maximum information from analytical ultracentrifugation experiments.
Demeler
Borries
B
Department of Biochemistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas.|Department of Computer Science, The University of Texas at San Antonio, San Antonio, Texas.
D004867:Equipment Design; D011506:Proteins / Q000737:chemistry*; D014461:Ultracentrifugation / Q000295:instrumentation* / Q000379:methods*
D011506:Proteins
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