title: OX40 induces CCL20 expression in the context of antigen stimulation: an expanding role of co-stimulatory molecules in chemotaxis.
abstract: OX40 is an inducible co-stimulatory molecule expressed by activated T cells. It plays an important role in the activation and proliferation of T lymphocytes. Recently, some co-stimulatory molecules have been shown to direct leukocyte trafficking. Chemotaxis is essential for achieving an effective immune response. CCL20 is an important chemoattractant produced by activated T cells. In this study, using DO11.10 mice whose transgenic T cell receptor specifically recognizes ovalbumin, we demonstrate that ovalbumin induces OX40 expression in CD4+ lymphocytes. Further stimulation of OX40 by OX40 activating antibody up-regulates CCL20 production. Both NF-kappaB dependent and independent signaling pathways are implicated in the induction of CCL20 by OX40. Finally, we primed the DO11.10 splenocytes with or without OX40 activating antibody in the presence of ovalbumin. Intranasal administration of the cell lysates derived from the cells with OX40 stimulation results in more severe leukocyte infiltration in the lung of DO11.10 mice, which is substantially attenuated by CCL20 blocking antibody. Taken together, this study has shown that activation of OX40 induces CCL20 expression in the presence of antigen stimulation. Thus, our results broaden the role of OX40 in chemotaxis, and reveal a novel effect of co-stimulatory molecules in orchestrating both T cell up-regulation and migration.
authors.affiliation: Department of Pediatrics, RuiJin Hospital, Shanghai 200025, China.
authors.forename: Wenwei
authors.initials: W
authors.lastname: Zhong
authors.forename: Zili
authors.initials: Z
authors.lastname: Zhang
authors.forename: David
authors.initials: D
authors.lastname: Hinrichs
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wu
authors.forename: Mark
authors.initials: M
authors.lastname: Hall
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Xia
authors.forename: James T
authors.initials: JT
authors.lastname: Rosenbaum
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002457:Cell Extracts; D054418:Chemokine CCL20 / Q000276:immunology*; D002633:Chemotaxis / Q000276:immunology*; D005260:Female; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D019570:Inhalation Exposure; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D009500:Neutralization Tests; D010047:Ovalbumin / Q000276:immunology*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D053262:Receptors, OX40 / Q000276:immunology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000906:Antibodies; D000941:Antigens; D002457:Cell Extracts; D054418:Chemokine CCL20; D016328:NF-kappa B; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors; D053262:Receptors, OX40; D010047:Ovalbumin; D048031:JNK Mitogen-Activated Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases
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