title: Analysis of spleen cells in malignant histiocytosis in infancy.
abstract: A 10-month-old male infant underwent splenectomy because of life-threatening hypersplenism due to malignant histiocytosis. The spleen tissue positive for herpes simplex virus (HSV)-DNA, histologically and immunologically revealed a marked B cell depletion replaced by proliferation of activated cytotoxic T cells. S100-positive histiomonocytoid cells and lysozyme-positive hemophagocytes were also significantly increased. No metaphases were obtained by cytogenetic studies and Southern blot analysis of spleen cell DNA demonstrated only germ bands of immunoglobulin and both T gamma and beta genes, providing no evidence of monoclonality in this case of so-called malignant histiocytosis.
authors.affiliation: Division of Pediatrics, Children's Research Hospital, Kyoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Imashuku
authors.forename: S
authors.initials: S
authors.lastname: Ikushima
authors.forename: T
authors.initials: T
authors.lastname: Yoshihara
authors.forename: S
authors.initials: S
authors.lastname: Todo
authors.forename: J
authors.initials: J
authors.lastname: Umemoto
authors.forename: T
authors.initials: T
authors.lastname: Hashida
authors.forename: S
authors.initials: S
authors.lastname: Fushiki
authors.forename: K
authors.initials: K
authors.lastname: Yumura-Yagi
authors.forename: K
authors.initials: K
authors.lastname: Ha-Kawa