[The pilot study on the correlation between human cytomegalovirus infection and the secretion of chemokines in the cultured human fibroblasts].
OBJECTIVE
To investigate the effect of HCMV infection on the expression of interleukin-8 (IL-8) and regulated on activation, normal T expressed and secreted (RANTES) so as to explore the possible mechanism of the immune-pathological changes caused by HCMV infection.


METHODS
Expression of both IL-8 and RANTES mRNA was detected by RT-PCR. Secretion of IL-8 and RANTES protein was quantitated by enzyme linked immune-sororbant assay (ELISA).


RESULTS
After HCMV infection, the expression of IL-8 in HEL cells was found to be increased gradually concomitant with the prolonged infection time at both the transcriptional level and the protein level. By the time of 72 h.p.i, as compared with mock-infected cells, IL-8 mRNA expression was increased up to 12-fold and IL-8 protein up to 9-fold in HCMV-infected cells. The expression of RANTES mRNA was occurred at 8 h.p.i, with a maximum at 24 h.p.i, since then it remained relatively high level. The expression of RANTES protein peaked at 24 h.p.i, then dropped sharply and was almost undetectable by the time at 72 h.p.i.


CONCLUSIONS
HCMV infection of HEL cells may induce the transcription of IL-8 gene as well as the production of IL-8 orotein. HCMV may down-regulate extra-cellular production of RANTES protein.
Zhang
Yan
Y
Department of Gynecology and Obstetrics, Third hospital, Peking University, Beijing 100083, China.
Lu
Shan
S
Zhang
Chun-yu
CY
Song
Xue-ling
XL
D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000276:immunology*; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D005260:Female; D005347:Fibroblasts / Q000276:immunology* / Q000821:virology; D015870:Gene Expression; D006801:Humans; D010865:Pilot Projects; D011247:Pregnancy
D018925:Chemokines