title: Variation of physiochemical properties and cell association activity of membrane vesicles with growth phase in Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa and other Gram-negative bacteria release membrane vesicles (MVs) from their surfaces, and MVs have an ability to interact with bacterial cells. Although it has been known that many bacteria have mechanisms that control their phenotypes with the transition from exponential phase to stationary phase, changes of properties in released MVs have been poorly understood. Here, we demonstrate that MVs released by P. aeruginosa during the exponential and stationary phases possess different physiochemical properties. MVs purified from the stationary phase had higher buoyant densities than did those purified from the exponential phase. Surface charge, characterized by zeta potential, of MVs tended to be more negative as the growth shifted to the stationary phase, although the charges of PAO1 cells were not altered. Pseudomonas quinolone signal (PQS), one of the regulators related to MV production in P. aeruginosa, was lower in MVs purified from the exponential phase than in those from the stationary phase. MVs from the stationary phase more strongly associated with P. aeruginosa cells than did those from the exponential phase. Our findings suggest that properties of MVs are altered to readily interact with bacterial cells along with the growth transition in P. aeruginosa.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Tashiro
authors.forename: Sosaku
authors.initials: S
authors.lastname: Ichikawa
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Shimizu
authors.forename: Masanori
authors.initials: M
authors.lastname: Toyofuku
authors.forename: Naoki
authors.initials: N
authors.lastname: Takaya
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakajima-Kambe
authors.forename: Hiroo
authors.initials: H
authors.lastname: Uchiyama
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Nomura
mesh_terms: D002499:Centrifugation, Density Gradient; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D015363:Quinolones / Q000032:analysis; D022142:Secretory Vesicles / Q000737:chemistry* / Q000378:metabolism*; D055672:Static Electricity
chemical_list: C407944:2-heptyl-3-hydroxy-4-quinolone; D015363:Quinolones
references.citation: BMC Microbiol. 2009 Feb 03;9:26
references.pmid: 19192306
references.citation: Microbes Environ. 2010;25(1):1-7
references.pmid: 21576845
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: J Electron Microsc (Tokyo). 2003;52(5):465-9
references.pmid: 14700078
references.citation: Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11229-34
references.pmid: 10500159
references.citation: Mol Microbiol. 2008 Jul;69(2):491-502
references.pmid: 18630345
references.citation: J Bacteriol. 2003 Apr;185(7):2080-95
references.pmid: 12644477
references.citation: J Bacteriol. 1998 May;180(9):2306-11
references.pmid: 9573179
references.citation: J Bacteriol. 1996 May;178(10):2767-74
references.pmid: 8631663
references.citation: Mol Microbiol. 2006 Dec;62(6):1689-99
references.pmid: 17083468
references.citation: Genes Cells. 1999 Mar;4(3):135-43
references.pmid: 10320479
references.citation: J Bacteriol. 2009 Jul;191(13):4097-102
references.pmid: 19429627
references.citation: Microbiol Rev. 1979 Mar;43(1):73-102
references.pmid: 111024
references.citation: J Bacteriol. 1998 Oct;180(20):5478-83
references.pmid: 9765585
references.citation: J Antimicrob Chemother. 2006 Apr;57(4):764-6
references.pmid: 16446373
references.citation: J Bacteriol. 2008 Dec;190(24):7947-56
references.pmid: 18931133
references.citation: J Bacteriol. 2006 Aug;188(16):5945-57
references.pmid: 16885463
references.citation: Microbes Infect. 2006 Aug;8(9-10):2400-8
references.pmid: 16807039
references.citation: Mol Microbiol. 2007 Jul;65(1):153-65
references.pmid: 17581126
references.citation: Mol Microbiol. 2003 Oct;50(1):29-43
references.pmid: 14507361
references.citation: Appl Environ Microbiol. 2001 Feb;67(2):491-4
references.pmid: 11157207
references.citation: J Bacteriol. 1995 Dec;177(24):7155-63
references.pmid: 8522523
references.citation: Anal Biochem. 1980 May 1;104(1):10-4
references.pmid: 6892980
references.citation: J Bacteriol. 1995 Jul;177(14):3998-4008
references.pmid: 7608073
references.citation: Microbiology (Reading). 2003 May;149(Pt 5):1311-1322
references.pmid: 12724392
references.citation: Infect Immun. 2008 Oct;76(10):4624-32
references.pmid: 18663005
references.citation: Antimicrob Agents Chemother. 2004 Apr;48(4):1320-8
references.pmid: 15047536
references.citation: Microbiology (Reading). 2003 Oct;149(Pt 10):2789-2795
references.pmid: 14523112
references.citation: FEMS Microbiol Lett. 2010 Mar;304(2):123-30
references.pmid: 20146747
references.citation: Nature. 2005 Sep 15;437(7057):422-5
references.pmid: 16163359
references.citation: Mol Microbiol. 2006 Aug;61(4):839-46
references.pmid: 16879642
references.citation: Proc Natl Acad Sci U S A. 2004 Feb 3;101(5):1339-44
references.pmid: 14739337
references.citation: J Bacteriol. 2007 May;189(10):3776-83
references.pmid: 17351035
references.citation: J Bacteriol. 1996 May;178(9):2479-88
references.pmid: 8626312
references.citation: J Bacteriol. 2009 Dec;191(24):7509-19
references.pmid: 19837799
references.citation: Microbiology (Reading). 2004 Jul;150(Pt 7):2161-2169
references.pmid: 15256559
references.citation: Mol Microbiol. 2006 Sep;61(5):1308-21
references.pmid: 16879411
references.citation: J Microbiol Methods. 2001 Jan;43(3):153-64
references.pmid: 11118650
references.citation: J Bacteriol. 2008 Jun;190(11):3969-78
references.pmid: 18390657