Fabrication of three-dimensional scaffolds for heterogeneous tissue engineering.
The development of biomedical scaffolds mimicking a heterogeneous cellular microenvironment for a specified regulation of cell-fates is very promising for tissue engineering. In this study, three-dimensional scaffolds with heterogeneous microstructure were developed using a DMD-PP apparatus. During the fabrication process, this apparatus can efficiently switch monomers to form microstructures with localized, different material properties; the resolution in the arrangement of material properties is comparable to the characteristic size of functional subunits in living organs, namely, a hundred microns. The effectiveness of this DMD-PP apparatus is demonstrated by a woodpile microstructure with heterogeneous fluorescence and also by a microporous cell-culturing scaffold with selected sites for protein adhesion. Cell-cultivation experiment was performed with the microporous scaffold, in which selective cell adhesion was observed.
Han
Li-Hsin
LH
Texas Materials Institute and Department of Mechanical Engineering, The University of Texas at Austin, C2200, Austin, TX 78712, USA.
Suri
Shalu
S
Schmidt
Christine E
CE
Chen
Shaochen
S
D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D056656:Lab-On-A-Chip Devices; D055616:Microtechnology / Q000295:instrumentation*; D011506:Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D011506:Proteins