Magnetic resonance spectroscopy of live Drosophila melanogaster using magic angle spinning.
High-Resolution Magic Angle Spinning (HRMAS) proton magnetic resonance spectroscopy ((1)H-MRS) is a novel non-destructive technique that improves spectral line-widths and allows high-resolution spectra to be obtained from extracts, intact cells, cell cultures, and more importantly intact tissue to investigate relationships between metabolites and cellular processes. In vivo HRMAS (1)H-MRS studies have yet to be reported in the live fruit fly Drosophila melanogaster. Drosophila, as a simpler genetic organism, allows the multiple biological functions and various evolutionarily conserved signaling pathways to be examined at the whole organism level and it is a useful model for investigating genetics and physiology. To this end, we developed and implemented an in vivo HRMAS (1)H-MRS method to investigate live Drosophila at 14.1 T. Here, we outline an HRMAS (1)H-MRS protocol for the molecular characterization of Drosophila with a conventional MR spectrometer equipped with an HRMAS probe. This technique is a novel, in vivo, non-destructive Drosophila metabolite measurement approach, which enables the identification of disease biomarkers and thus may contribute to novel therapeutic development.
Righi
Valeria
V
NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, MA, USA.
Apidianakis
Yiorgos
Y
Rahme
Laurence G
LG
Tzika
A Aria
AA
D000818:Animals; D004331:Drosophila melanogaster / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*
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