Validation of HPLC method for the simultaneous and quantitative determination of 12 UV-filters in cosmetics.
The aim of the study was the validation of a high-performance liquid chromatography (HPLC) method for the simultaneous and quantitative determination of twelve commonly used organic UV-filters (phenylbenzimidazole sulfonic acid, benzophenone-3, isoamyl p-methoxycinnamate, diethylamino hydroxybenzoyl hexyl benzoate, octocrylene, ethylhexyl methoxycinnamate, ethylhexyl salicylate, butyl methoxydibenzoylmethane, diethylhexyl butamido triazone, ethylhexyl triazone, methylene bis-benzotriazolyl tetramethylbutylphenol and bis-ethylhexyloxyphenol methoxyphenyl triazine) contained in suncare products. The separation and quantitative determination was performed in <30 min, using a Symmetry Shield(R) C18 (5 microm) column from Waters and a mobile phase (gradient mode) consisting of ethanol and acidified water. UV measurements were carried out at multi-wavelengths, according to the absorption of the analytes.
Nyeborg
M
M
Laboratoire COTY-LANCASTER, Recherche et développement analytique, 6, avenue Albert II, 98000 Monaco, France. monika_nyeborg@cotyinc.com
Pissavini
M
M
Lemasson
Y
Y
Doucet
O
O
D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003358:Cosmetics / Q000737:chemistry*; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D013473:Sunscreening Agents / Q000032:analysis*
D003358:Cosmetics; D013473:Sunscreening Agents