title: Trichostatin A inhibits expression of cathepsins in experimental osteoarthritis.
abstract: The aim of this study was to investigate the effects of trichostatin A (TSA) on expression of cathepsins in cartilage in experimental osteoarthritis (OA). OA was induced in 18 rabbits by bilateral anterior cruciate ligament transection (ACLT). Four weeks after surgery, rabbits received intra-articular injection with TSA dissolved in the dimethylsulphoxide (DMSO) in the right knees and DMSO in the left knees once a week for 5 weeks. Rabbits were killed 7 days after the last injection. The knee joints were assessed by morphological and histological examination. Messenger RNA expression of cathepsins K, B, L, S and cystatin C was studied by real-time PCR. TSA inhibited the expression of cathepsins K, B, L, S and cystatin C accompanied with the less degradation in cartilage. The results suggest that TSA exhibits protective effects against cartilage degradation in rabbits with OA and the effects may be associated with the inhibition of cathepsins.
authors.affiliation: Department of Orthopedics Surgery, The Second Hospital of Medical College, Zhejiang University, Jie Fang Road 88#, 310009 Hangzhou, People’s Republic of China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Chen
authors.forename: Jia-Peng
authors.initials: JP
authors.lastname: Bao
authors.forename: Jin-Li
authors.initials: JL
authors.lastname: Tang
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Hu
authors.forename: Li-Dong
authors.initials: LD
authors.lastname: Wu
mesh_terms: D000818:Animals; D002403:Cathepsins / Q000037:antagonists & inhibitors* / Q000235:genetics*; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008297:Male; D020370:Osteoarthritis, Knee / Q000188:drug therapy* / Q000201:enzymology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D006877:Hydroxamic Acids; D011480:Protease Inhibitors; C012589:trichostatin A; D002403:Cathepsins
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