Anaplasma phagocytophilum from Rodents and Sheep, China.
To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China. We isolated 3 strains (2 from rodents and 1 from sick sheep) through propagation in BALB/c mice and then cell culture in HL60 cells. The 3 isolates were identified by Wright-Giemsa staining, immunofluorescence, and electronic microscopy and were characterized by sequence analyses of the 16S rRNA gene, partial citrate synthase gene, major surface protein 4 gene, and heat shock protein gene. The multiple sequences of the 3 isolates were identical to each other but different from all known strains from other countries. The public health and veterinary relevance of the isolates deserves further investigation.
Zhan
Lin
L
Beijing Institute of Microbiology and Epidemiology, Beijing, People's Republic of China.
Cao
Wu Chun
WC
Jiang
Jia Fu
JF
Zhang
Xiao Ai
XA
Liu
Yun Xi
YX
Wu
Xiao Ming
XM
Zhang
Wen Yi
WY
Zhang
Pan He
PH
Bian
Chang Ling
CL
Dumler
J Stephen
JS
Yang
Hong
H
Zuo
Shu Qing
SQ
Chu
Chen Yi
CY
Liu
Wei
W
Richardus
Jan H
JH
Habbema
J Dik F
JD
D041081:Anaplasma phagocytophilum / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D002681:China / Q000453:epidemiology; D002950:Citrate (si)-Synthase / Q000032:analysis / Q000235:genetics; D006224:Cricetinae; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016873:Ehrlichiosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D018922:HL-60 Cells; D006360:Heat-Shock Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012376:Rodent Diseases / Q000453:epidemiology / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology*
D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D012336:RNA, Ribosomal, 16S; D002950:Citrate (si)-Synthase
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