title: Characterization of new serum biomarkers in breast cancer using lipid microarrays.
abstract: Breast cancer is the most common form of cancer among women. Compared with other serum polypeptides, autoantibodies have many appealing features as biomarkers including sensitivity, stability, and easy detection. Anti-lipid autoantibodies are routinely used in the diagnosis of autoimmune diseases, but their potential for cancer diagnosis has not been explored. Dysregulation of cellular signaling in cancer cells would be expected to lead to irregular metabolism of many lipids, which could be sensed by the immune system and cause the production of autoantibodies. Discovery of anti-lipid antibodies could be used as biomarkers for early breast cancer diagnosis. We describe here a more sensitive and accurate method for lipid microarray detection using dual fluorescent labeling, and used it to examine global anti-lipid profiles in the MMTV-Neu transgenic breast cancer model. We conclude that, at the current technology, lipid microarray is not a preferred method for anti-lipid antibody detection in breast cancer animal models. Our result will help the future application of lipid microarrays in identifying anti-lipid autoantibodies in breast cancer and other human diseases.
authors.affiliation: Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Yonekubo
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Aimalohi
authors.initials: A
authors.lastname: Esechie
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guangwei
authors.initials: G
authors.lastname: Du
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood* / Q000276:immunology; D001943:Breast Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D008055:Lipids / Q000276:immunology*; D051379:Mice; D046228:Microarray Analysis / Q000379:methods*
chemical_list: D001323:Autoantibodies; D014408:Biomarkers, Tumor; D008055:Lipids
references.citation: Mol Cell Proteomics. 2006 Oct;5(10):1745-59
references.pmid: 16733262
references.citation: N Engl J Med. 2005 Sep 22;353(12):1224-35
references.pmid: 16177248
references.citation: JAMA. 2002 Apr 3;287(13):1662-70
references.pmid: 11926890
references.citation: Mol Cancer Res. 2003 Aug;1(10):707-15
references.pmid: 12939396
references.citation: Anal Chem. 2008 Nov 1;80(21):7980-7
references.pmid: 18841940
references.citation: Annu Rev Pathol. 2006;1:243-71
references.pmid: 18039115
references.citation: Rapid Commun Mass Spectrom. 2009 Mar;23(6):863-76
references.pmid: 19224569
references.citation: Clinics (Sao Paulo). 2005 Jun;60(3):257-8
references.pmid: 15962088
references.citation: J Cell Biochem. 2004 Jan 1;91(1):47-53
references.pmid: 14689581
references.citation: Proteome Sci. 2006 Apr 04;4:5
references.pmid: 16595007
references.citation: Int J Cancer. 2005 Sep 1;116(3):368-77
references.pmid: 15818621
references.citation: Ann Hematol. 2002 Dec;81(12):727-31
references.pmid: 12483370
references.citation: Breast Cancer Res. 2007;9(2):206
references.pmid: 17397520
references.citation: Trends Biotechnol. 2008 Dec;26(12):647-52
references.pmid: 18951641
references.citation: Cancer Res. 2003 Nov 1;63(21):7365-76
references.pmid: 14612535
references.citation: Nat Med. 2006 Jan;12(1):138-43
references.pmid: 16341241
references.citation: Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10578-82
references.pmid: 1359541
references.citation: Mol Pharm. 2006 Sep-Oct;3(5):496-506
references.pmid: 17009848