Pegylated poly-l-arginine derivatives of chitosan for effective delivery of siRNA.
For delivery of siRNA, chitosan (CS) was derivatized with poly-l-arginine (PLR) and polyethylene glycol (PEG). The formation of polyplexes with siRNA was confirmed by gel retardation. The PLR-grafted CS formed nanosized particles with siRNA. PLR-grafted CS showed higher cellular delivery efficiency of siRNA than did CS, pegylated CS, PLR, or pegylated PLR. The extent of reduction in the expression of fluorescent proteins was highest following treatment of the cells using PLR derivatives of CS in complexes with specific siRNAs. Cell viability was greater in populations treated with pegylated CS-PLR than in those treated with PLR. Hemolysis of erythrocytes was reduced upon conjugation of PLR with CS. The delivery of siRNAs via pegylated CS-PLR revealed little dependence on serum. Molecular imaging techniques revealed that the intratumoral administration of red fluorescent protein-specific siRNA in complexes with pegylated CS-PLR significantly silenced the expression of red fluorescent proteins in tumor tissues in vivo. These results indicate that pegylated CS-PLR might be useful for in vivo delivery of therapeutic siRNAs.
Noh
Sang Myoung
SM
College of Pharmacy, Seoul National University, Sinlim-dong, Gwanak-gu, Seoul, South Korea.
Park
Myung Ok
MO
Shim
Gayong
G
Han
Su Eun
SE
Lee
Han Young
HY
Huh
Jun Hyuk
JH
Kim
Myoung Suk
MS
Choi
Jin Joo
JJ
Kim
Kwangmeyung
K
Kwon
Ick Chan
IC
Kim
Jin-Seok
JS
Baek
Kwang-Hyun
KH
Oh
Yu-Kyoung
YK
D000818:Animals; D001120:Arginine / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000628:therapy; D008175:Lung Neoplasms / Q000473:pathology / Q000628:therapy; D051379:Mice; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D034741:RNA, Small Interfering / Q000008:administration & dosage*
D004337:Drug Carriers; D011108:Polymers; D034741:RNA, Small Interfering; D011092:Polyethylene Glycols; D048271:Chitosan; D001120:Arginine