Glycosaminoglycan chain of dentin sialoprotein proteoglycan.
Dentin sialophosphoprotein (DSPP) is processed into dentin sialoprotein (DSP) and dentin phosphoprotein. A molecular variant of rat DSP, referred to as "HMW-DSP", has been speculated to be a proteoglycan form of DSP. To determine if HMW-DSP is the proteoglycan form of DSP and to identify the glycosaminoglycan side-chain attachment site(s), we further characterized HMW-DSP. Chondroitinase ABC treatment reduced the migration rate for portions of rat HMW-DSP to the level of DSP. Disaccharide analysis showed that rat HMW-DSP contains glycosaminoglycan chains made of chondroitin-4-sulfate and has an average of 31-32 disaccharides/mol. These observations confirmed that HMW-DSP is the proteoglycan form of DSP (renamed "DSP-PG"). Edman degradation and mass spectrometric analyses of tryptic peptides from rat DSP-PG, along with substitution analyses of candidate Ser residues in mouse DSPP, confirmed that 2 glycosaminoglycan chains are attached to Ser(241) and Ser(253) in the rat, or Ser(242) and Ser(254) in the mouse DSPP sequence.
Zhu
Q
Q
Department of Biomedical Sciences, Baylor College of Dentistry, Texas A&M Health Science Center, 3302 Gaston Ave., Dallas, TX 75246, USA.
Sun
Y
Y
Prasad
M
M
Wang
X
X
Yamoah
A K
AK
Li
Y
Y
Feng
J
J
Qin
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002809:Chondroitin Sulfates / Q000032:analysis; D003804:Dentin / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry; D016326:Extracellular Matrix Proteins / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis; D010750:Phosphoproteins / Q000737:chemistry*; D011499:Protein Processing, Post-Translational; D011509:Proteoglycans / Q000737:chemistry*; D051381:Rats; D012694:Serine / Q000737:chemistry; D012795:Sialoglycoproteins / Q000737:chemistry*; D000099311:Dentin Sialophosphoprotein
D002809:Chondroitin Sulfates; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; D010455:Peptides; D010750:Phosphoproteins; D011509:Proteoglycans; D012694:Serine; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein
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