Exposure of brown trout (Salmo trutta L.) to tunnel wash water runoff--chemical characterisation and biological impact.
Washing and cleaning of road tunnels are a routinely performed maintenance task, which generate significant amount of polluted wash-water runoff that normally is discharged to the nearest recipient. The present study was designed to quantify chemical contaminants (trace metals, hydrocarbons, PAH and detergents) in such wash water and assess the short term impact on brown trout (Salmo trutta L.) based on in situ experiments. Selected endpoints were accumulation of trace metals in gills, haematological variables and hepatic mRNA transcription of five biomarkers reflecting defence against free radicals, trace metals, planar aromatic hydrocarbons and endocrine disruptions which were measured prior (-3h), during (1 and 3h) and after the tunnel wash (14, 38 and 86h). Our findings showed that the runoff water was highly polluted, but most of the contaminants were associated with particles which are normally considered biologically inert. In addition, high concentrations of calcium and dissolved organic carbon were identified in the wash water, thus reducing metal toxicity. However, compared to the control fish, a rapid accumulation of trace metals in gills was observed. This was immediately followed by a modest change in blood ions and glucose in exposed fish shortly after the exposure start. However, after 38-86h post wash, gill metal concentrations, plasma ions and glucose levels recovered back to control levels. In contrast, the mRNA transcription of the CYP1A and the oxidative stress related biomarkers TRX and GCS did not increase until 14h after the exposure start and this increase was still apparent when the experiment was terminated 86h after the beginning of the tunnel wash. The triggering of the defence systems seemed to have successfully restored homeostasis of the physiological variables measured, but the fish still used energy for detoxification four days after the episode, measured as increased biomarker synthesis.
Meland
Sondre
S
Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, As, Norway. sondre.meland@umb.no
Heier
Lene Sørlie
LS
Salbu
Brit
B
Tollefsen
Knut Erik
KE
Farmen
Eivind
E
Rosseland
Bjørn Olav
BO
D000818:Animals; D003902:Detergents / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D004784:Environmental Monitoring; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008670:Metals / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D014337:Trout / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*
D003902:Detergents; D008670:Metals; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical