[Polymorphisms of vascular diseases-related genes in Guangzhou Biobank Cohort Study].
OBJECTIVE
To study the polymorphisms of cerebrovascular and cardiovascular disease genes using Taqman single nucleotide polymorphism (SNP) genotyping kits.


METHODS
A total of 2000 subjects were recruited from the Guangzhou Biobank Cohort Study (GBCS), and 15 SNPs were detected using Taqman SNP genotyping kits and an ABI 7900HT real time PCR system. The data were tested for the Hardy-Weinberg equilibrium, and then compared with the data of the Chinese population from the International HapMap Project (HapMap_HCN).


RESULTS
(1) All genotype data of the 15 SNPs were consistent with the Hardy-Weinberg rules. (2) The significant differences were observed among two SNPs, rs4220 and rs5368 and the HapMap_HCN (rs4220 28.2% vs 17.8%; chi(2) = 4.891, P = 0.028; rs5368 22.1% vs 32.2%, chi(2) = 5.137, P = 0.024). Comparing other gene bank data, such as AFD-CHN-PANEL, the Allele Frequency Database (ALFRED) and JBIC-allele, it would be most likely that our observations represent differences between the Northern and Southern populations in China.


CONCLUSIONS
Such Biobank study provided a useful platform for the study of the role of genetic and environmental determinants on cerebrovascular and cardiovascular disease.
Liu
Bin
B
Molecular Epidemiological Research Center, Guangzhou No.12 Hospital, Guangzhou 510620, China.
Jiang
Chao-Qiang
CQ
Thomas
Graham Neil
GN
Lao
Xiang-Qian
XQ
Lin
Jie-Ming
JM
Yue
Xiao-Jun
XJ
Zhang
Wei-Sen
WS
Zhu
Tong
T
Lin
Da-Qing
DQ
D044466:Asian People / Q000235:genetics; D018070:Biological Specimen Banks / Q000706:statistics & numerical data*; D001927:Brain Diseases / Q000453:epidemiology / Q000235:genetics*; D002318:Cardiovascular Diseases / Q000453:epidemiology / Q000235:genetics*; D002681:China / Q000453:epidemiology; D015331:Cohort Studies; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide