title: Prediction of polyphenol oxidase activity using visible near-infrared hyperspectral imaging on mushroom (Agaricus bisporus) caps.
abstract: Physical stress (i.e., bruising) during harvesting, handling, and transportation triggers enzymatic discoloration of mushrooms, a common and detrimental phenomenon largely mediated by polyphenol oxidase (PPO) enzymes. Hyperspectral imaging (HSI) is a nondestructive technique that combines imaging and spectroscopy to obtain information from a sample. The objective of this study was to assess the ability of HSI to predict the activity of PPO on mushroom caps. Hyperspectral images of mushrooms subjected to various damage treatments were taken, followed by enzyme extraction and PPO activity measurement. Principal component regression (PCR) models (each with three PCs) built on raw reflectance and multiple scatter-corrected (MSC) reflectance data were found to be the best modeling approach. Prediction maps showed that the MSC model allowed for compensation of spectral differences due to sample curvature and surface irregularities. Results reveal the possibility of developing a sensor that could rapidly identify mushrooms with a higher likelihood to develop enzymatic browning, hence aiding produce management decision makers in the industry.
authors.affiliation: School of Food Science and Environmental Health, Dublin Institute of Technology, Cathal Brugha Street, Dublin 1, Ireland.
authors.forename: Edurne
authors.initials: E
authors.lastname: Gaston
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Frías
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Cullen
authors.forename: Colm P
authors.initials: CP
authors.lastname: O'Donnell
authors.forename: Aoife A
authors.initials: AA
authors.lastname: Gowen
mesh_terms: D000364:Agaricus / Q000201:enzymology*; D004156:Catechol Oxidase / Q000378:metabolism*; D004789:Enzyme Activation; D005524:Food Technology / Q000379:methods; D015416:Maillard Reaction; D014442:Monophenol Monooxygenase / Q000378:metabolism; D012044:Regression Analysis; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase