title: Dangshen (Codonopsis pilosula) activates IGF-I and FGF-2 pathways to induce proliferation and migration effects in RSC96 Schwann cells.
abstract: This study evaluates the proliferative and migrative effects of dangshen on RSC96, Schwann cells. We investigated the molecular signaling pathways, which include: (1) survival signaling, IGFs-IGFIR-Akt-Bcl2 and proliferative signaling, cell cycle factors and MAPK pathways. (2) migrate and anti-scar signaling, FGF-2-uPA-MMPs. After treatment with different concentrations (20 microg/ml, 40 microg/ml, 60 microg/ml, 80 microg/ml, and 100 microg/ml) of dangshen. We observed a dose dependent proliferative effect using PCNA Western blotting assay, MTT assay and the wound healing test. We also found that dangshen stimulates the protein expressions of IGF-I pathway regulators, cell cycle controlling proteins and excites the MAPK signaling pathway regulators ERK and P38. Dangshen even stimulates the FGF-2-uPA-MMP 9 migration pathway in RSC 96 Schwann cells. Using MAPK chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of dangshen on RSC 96 cells were identified to be ERK- and P38- dependent. Based on these results, applying an appropriate dose of dangshen with biomedical materials would be a potential approach for enhancing neuron regeneration.
authors.affiliation: Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan.
authors.forename: Hsien-Tung
authors.initials: HT
authors.lastname: Chen
authors.forename: Ying-Lan
authors.initials: YL
authors.lastname: Tsai
authors.forename: Yueh-Sheng
authors.initials: YS
authors.lastname: Chen
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Jong
authors.forename: Wei-Kung
authors.initials: WK
authors.lastname: Chen
authors.forename: Hwai-Lee
authors.initials: HL
authors.lastname: Wang
authors.forename: Fuu-Jen
authors.initials: FJ
authors.lastname: Tsai
authors.forename: Chang-Hai
authors.initials: CH
authors.lastname: Tsai
authors.forename: Tung-Yuan
authors.initials: TY
authors.lastname: Lai
authors.forename: Bor-Show
authors.initials: BS
authors.lastname: Tzang
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
authors.forename: Chung-Yen
authors.initials: CY
authors.lastname: Lu
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D029745:Codonopsis / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I