Parylene coating hinders Candida albicans adhesion to silicone elastomers and denture bases resin.
OBJECTIVE
To investigate whether parylene coatings over denture bases and silicone elastomers can effectively reduce Candida albicans adhesion and thus to decrease the incidence of denture stomatitis.


METHODS
Specimens of silicone elastomers A-2186 or lucitone 199 resin were prepared, and the measurements of contact angle, assay of XTT reduction and cell count of C. albicans adhesion were taken before and after parylene treatment. Furthermore, morphology of C. albicans adhesion for 48 h was observed by scanning electron microscope (SEM), and C. albicans adhesion for 4h was illustrated by confocal scanning laser microscopy (CLSM) in combination with fluorescent dyes FUN-1 and Concanavalin A.


RESULTS
There was a statistical difference between mean contact angles of silicone elastomer A-2186 before and after parylene coating (P<0.05). The amount of C. albicans adhesion to the surface of silicone elastomer A-2186 and lucitone 199 resin after parylene treatment was significantly less than before parylene treatment by cells count and XTT reduction assay (P<0.05). In SEM and CLSM analysis, C. albicans biofilm was more apt to generate on the surface of silicone elastomer A-2186 than other three groups, and more C. albicans aggregation formed on the surface of silicone elastomer A-2186 and lucitone 199 resin before parylene treatment than after parylene treatment.


CONCLUSIONS
Parylene coating reduced C. albicans adhesion and aggregation on the surface of silicone elastomer A-2186 and lucitone 199 resin, and improved the wettability of silicone elastomer A-2186.
Zhou
Lin
L
Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Tong
Zhongchun
Z
Wu
Guofeng
G
Feng
Zhihong
Z
Bai
Shizhu
S
Dong
Yan
Y
Ni
Longxing
L
Zhao
Yimin
Y
D000180:Acrylic Resins; D018441:Biofilms / Q000187:drug effects; D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects*; D020099:Coated Materials, Biocompatible; D003819:Denture Bases / Q000382:microbiology*; D004129:Dimethylpolysiloxanes; D008422:Materials Testing; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D011108:Polymers; D012826:Silicone Elastomers; D017155:Wettability; D014992:Xylenes
C076788:A 2186; D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D011108:Polymers; D012826:Silicone Elastomers; D014992:Xylenes; C011055:parylene; C040291:Lucitone