A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection.
We present a de novo design of an objective for use in multi-photon (MPM) and second harmonic generation (SHG) microscopy. This objective was designed to have a large field of view (FOV), while maintaining a moderate numerical aperture (NA) and relative straight forward construction. A dichroic beam splitter was incorporated within the objective itself allowing for an increase in the front aperture of the objective and corresponding enhancement of the solid angle of collected emission by an order of magnitude over existing designs.
McMullen
Jesse D
JD
School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA.
Zipfel
Warren R
WR
D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*
Nat Biotechnol. 2003 Nov;21(11):1369-77
14595365
Opt Express. 2009 Apr 13;17(8):6421-35
19365467
J Opt Soc Am A Opt Image Sci Vis. 2006 Dec;23(12):3139-49
17106469
J Microsc. 2007 Dec;228(Pt 3):330-7
18045327
Appl Opt. 2002 Sep 1;41(25):5376-82
12211567
Opt Express. 2007 Aug 6;15(16):10154-62
19547364
Opt Lett. 2006 Aug 15;31(16):2447-9
16880851
Opt Express. 2005 Jul 11;13(14):5528-34
19498549
Clin Gastroenterol Hepatol. 2008 Jan;6(1):95-101
18065276
Science. 1990 Apr 6;248(4951):73-6
2321027
Proc SPIE Int Soc Opt Eng. 2009;7161:
19360140
Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7075-80
12756303
Opt Lett. 2004 Nov 1;29(21):2521-3
15584281