Synthetic biology: applications come of age.
Synthetic biology is bringing together engineers and biologists to design and build novel biomolecular components, networks and pathways, and to use these constructs to rewire and reprogram organisms. These re-engineered organisms will change our lives over the coming years, leading to cheaper drugs, 'green' means to fuel our cars and targeted therapies for attacking 'superbugs' and diseases, such as cancer. The de novo engineering of genetic circuits, biological modules and synthetic pathways is beginning to address these crucial problems and is being used in related practical applications.
Khalil
Ahmad S
AS
Howard Hughes Medical Institute, Department of Biomedical Engineering, Center for BioDynamics and Center for Advanced Biotechnology, Boston University, Boston, Massachusetts 02215, USA.
Collins
James J
JJ
D000818:Animals; D001672:Biocompatible Materials; D056804:Biofuels; D015374:Biosensing Techniques; D001709:Biotechnology / Q000639:trends*; D004358:Drug Therapy; D053263:Gene Regulatory Networks; D005818:Genetic Engineering; D006801:Humans; D013678:Technology, Pharmaceutical
D001672:Biocompatible Materials; D056804:Biofuels
Biotechnol Bioeng. 2006 May 5;94(1):1-4
16534780
Genes Dev. 1999 Mar 15;13(6):655-65
10090722
Cell Res. 2007 Nov;17(11):963-5
17971808
J Am Chem Soc. 2009 May 13;131(18):6508-15
19378995
J Theor Biol. 1974 Mar;44(1):167-90
4595774
Nat Biotechnol. 2004 Jul;22(7):867-70
15184906
J Exp Med. 2007 Sep 3;204(9):2047-51
17709424
Cell. 2007 Sep 7;130(5):797-810
17803904
Cell. 1983 Dec;35(3 Pt 2):849-57
6197186
Science. 2000 Feb 4;287(5454):820-5
10657289
Nat Biotechnol. 2004 Jul;22(7):841-7
15208640
Science. 2008 Oct 17;322(5900):456-60
18927397
Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):425-30
16407165
Genes Dev. 2007 Sep 15;21(18):2271-6
17875664
Science. 2002 May 24;296(5572):1466-70
12029133
Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4629-34
19255432
Nature. 2009 Oct 15;461(7266):997-1001
19749742
J R Soc Interface. 2009 May 6;6(34):463-9
18835803
Nat Biotechnol. 2002 Sep;20(9):901-7
12205509
J Theor Biol. 1973 Apr;39(1):103-29
4741704
Nature. 2008 Jan 3;451(7174):86-9
18172501
Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6355-60
15096621
Science. 2008 Feb 29;319(5867):1215-20
18218864
Cell. 2009 Jun 26;137(7):1272-81
19563759
Mol Syst Biol. 2007;3:133
17700541
Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9517-22
15972812
Nature. 2005 Nov 24;438(7067):441-2
16306980
Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10135-40
19502423
Microbiol Rev. 1986 Dec;50(4):484-524
3540574
Nature. 2004 Mar 18;428(6980):281-6
15029187
Nature. 2000 Jan 20;403(6767):335-8
10659856
Cell. 2002 Nov 27;111(5):747-56
12464185
Science. 1995 Aug 4;269(5224):650-6
7624793
Nucleic Acids Res. 2003 Jun 15;31(12):e69
12799458
Trends Genet. 1999 Nov;15(11):439-42
10529804
Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8414-9
15159530
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6008-12
6171808
Nature. 2010 Jan 28;463(7280):559-62
20111002
Nature. 2005 May 5;435(7038):118-22
15875027
EMBO Rep. 2003 Feb;4(2):159-65
12612605
Proc Natl Acad Sci U S A. 1989 Jul;86(14):5400-4
2664783
Science. 2009 Oct 23;326(5952):589-92
19900898
Nature. 2009 Jul 9;460(7252):220-4
19536156
Nucleic Acids Res. 2002 Oct 15;30(20):e108
12384610
Cell. 2008 Jun 13;133(6):1043-54
18555780
Nat Biotechnol. 2007 Jul;25(7):795-801
17515909
Cell. 1993 Dec 3;75(5):843-54
8252621
Nucleic Acids Res. 2003 Jul 15;31(14):e71
12853648
J Bacteriol. 2003 Mar;185(6):1923-34
12618456
Nat Biotechnol. 2000 Nov;18(11):1203-8
11062442
Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19944-9
19036930
Trends Biotechnol. 2008 Jul;26(7):375-81
18471913
Science. 1990 Aug 3;249(4968):505-10
2200121
Cell. 1987 Feb 27;48(4):555-66
3028641
Proc Natl Acad Sci U S A. 2008 Jul 22;105(29):9994-8
18621677
Cell Metab. 2009 Jun;9(6):525-36
19490907
Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12678-83
16123130
Nat Biotechnol. 2001 Nov;19(11):1035-41
11689848
Science. 2009 May 29;324(5931):1199-202
19478183
Cell Mol Life Sci. 2003 May;60(5):854-61
12827275
Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):9919-24
10954740
Cell. 1999 Jan 22;96(2):271-90
9988221
Nature. 2004 Apr 22;428(6985):868-71
15064770
Nature. 2003 May 8;423(6936):185-90
12736688
Nat Rev Genet. 2001 Dec;2(12):919-29
11733745
Nucleic Acids Res. 2005 Jul 07;33(12):e107
16002786
Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547-51
1319065
Cell. 2002 Sep 6;110(5):551-61
12230973
Science. 2009 Sep 25;325(5948):1693-6
19696314
Nature. 1990 Aug 30;346(6287):818-22
1697402
Nat Rev Genet. 2009 Dec;10(12):859-71
19898500
Science. 2008 Jun 6;320(5881):1313-7
18467556
Mol Syst Biol. 2007;3:91
17353933
Nat Biotechnol. 2006 May;24(5):545-54
16680139
Cell. 2008 Nov 14;135(4):679-90
19013277
Nucleic Acids Res. 2009 Mar;37(4):e33
19190091
Mol Microbiol. 2002 Nov;46(3):813-26
12410838
Nature. 2004 Sep 23;431(7007):471-6
15386015
Science. 2003 Sep 26;301(5641):1904-8
14512628
Annu Rev Microbiol. 1994;48:713-42
7826024
Syst Synth Biol. 2007 Apr;1(2):89-98
19003438
Nature. 2003 Apr 10;422(6932):633-7
12687005
Nature. 2008 Nov 27;456(7221):516-9
18971928
PLoS One. 2008 Jul 30;3(7):e2815
18665232
Nature. 2000 Jan 20;403(6767):339-42
10659857
Nat Biotechnol. 2005 Mar;23(3):337-43
15723047
Nat Biotechnol. 2000 Apr;18(4):429-32
10748525
Mol Syst Biol. 2009;5:309
19756048
Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2643-8
17296937
Annu Rev Microbiol. 2005;59:487-517
16153177
Nat Biotechnol. 2009 Aug;27(8):753-9
19648908
Nature. 2002 Oct 31;419(6910):952-6
12410317
Science. 2007 Aug 3;317(5838):632-8
17600181
Metab Eng. 2006 May;8(3):273-80
16473537
Nature. 2002 Jul 11;418(6894):222-8
12110898
Nat Biotechnol. 2009 May;27(5):465-71
19377462
Cell. 2003 May 30;113(5):577-86
12787499
Cell. 2007 Jul 27;130(2):363-72
17662949
Cell. 1982 Nov;31(1):147-57
6297745
Curr Biol. 2000 Apr 20;10(8):R318-20
10801411
J Theor Biol. 1975 Oct;54(1):85-107
1202295
J Mol Biol. 2006 Jan 27;355(4):619-27
16330045
Nature. 2010 Jan 21;463(7279):326-30
20090747
Cell. 1987 Jun 5;49(5):603-12
3034429
Nature. 2005 Apr 28;434(7037):1130-4
15858574
Nature. 1974 Dec 13;252(5484):546-9
4431516
Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15573-8
15496466
Nat Rev Microbiol. 2009 Oct;7(10):715-23
19756010
Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11197-202
17592147
Curr Opin Biotechnol. 2006 Oct;17(5):548-57
16978856
Science. 2008 Mar 14;319(5869):1539-43
18339942
Nat Biotechnol. 2009 Oct;27(10):946-50
19801975
Appl Microbiol Biotechnol. 2008 Jan;77(6):1305-16
18060402
Proc Natl Acad Sci U S A. 2005 Mar 8;102(10):3581-6
15738412
Nat Biotechnol. 2004 Nov;22(11):1440-4
15502819
Nature. 2006 Feb 16;439(7078):856-60
16482159
Nature. 2009 Aug 13;460(7257):894-898
19633652
Nat Biotechnol. 2003 Sep;21(9):1069-74
12923549
Nature. 2006 Apr 13;440(7086):940-3
16612385
Nat Biotechnol. 2003 Jul;21(7):796-802
12778056
Cold Spring Harb Symp Quant Biol. 1961;26:389-401
14475415
Cell. 2003 May 30;113(5):597-607
12787501
Nat Cell Biol. 2009 Sep;11(9):1093-102
19684579
Science. 2005 Oct 7;310(5745):77-80
16210530
Nature. 2009 Jan 15;457(7227):309-12
19148099
BMC Biotechnol. 2006 Nov 03;6:43
17083727
J Am Chem Soc. 2005 Dec 21;127(50):17584-5
16351070
Nat Biotechnol. 2009 Dec;27(12):1139-50
20010597
Nucleic Acids Res. 1997 Mar 15;25(6):1203-10
9092630