Correlation between Klebsiella pneumoniae carrying pLVPK-derived loci and abscess formation.
Klebsiella pneumoniae-caused liver abscess (KLA) is an emerging infectious disease. However, factors other than K1-specific loci that contribute to the pathogenesis of this disease have not been identified. pLVPK is a 219,385-bp plasmid of K. pneumoniae CG43, an invasive K2 strain associated with KLA. We aimed in this study to evaluate the involvement of pLVPK in K. pneumoniae virulence and its clinical significance in abscess formation. A pLVPK-cured CG43 was isolated and its virulence was examined in a mouse model. The prevalence of pLVPK-derived loci terW, iutA, rmpA, silS, and repA was investigated in 207 clinical isolates by screening with specific primers. Loss of pLVPK abolished the ability of K. pneumoniae to disseminate into extraintestinal sites and, consequently, attenuated abscess formation in mice. Primary K. pneumoniae abscess isolates (n = 94) were more likely to be terW (+)-iutA (+)-rmpA (+)-silS (+) than those related to non-abscess infections (n = 113) (62% vs. 27%; p < 0.0001). Logistic regression analysis indicated that the presence of the terW-rmpA-iutA-silS loci was a significant risk factor (odds ratio, 4.12; 95% confidence interval, 2.02-8.4; p < 0.0001) for abscess formation. pLVPK is a determinant for K. pneumoniae virulence and infection with strains carrying the pLVPK-derived terW-rmpA-iutA-silS loci may predispose patients to abscess formation.
Tang
H-L
HL
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, No. 250, Guoguang Road, Taichung 402, Taiwan, Republic of China.
Chiang
M-K
MK
Liou
W-J
WJ
Chen
Y-T
YT
Peng
H-L
HL
Chiou
C-S
CS
Liu
K-S
KS
Lu
M-C
MC
Tung
K-C
KC
Lai
Y-C
YC
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D008100:Liver Abscess / Q000382:microbiology*; D008297:Male; D051379:Mice; D008875:Middle Aged; D010957:Plasmids / Q000032:analysis*; D017384:Sequence Deletion; D037521:Virulence Factors / Q000235:genetics*
D001426:Bacterial Proteins; D037521:Virulence Factors
Diagn Microbiol Infect Dis. 2005 Jun;52(2):79-84
15964493
Hepatol Res. 2002 Apr;22(4):307-312
11929716
Eur J Clin Microbiol. 1984 Dec;3(6):506-9
6396090
Emerg Infect Dis. 2002 Feb;8(2):160-6
11897067
Infect Immun. 1986 Dec;54(3):603-8
2946641
Clin Infect Dis. 2007 Dec 1;45(11):1530-1; author reply 1532-3
17990243
J Infect. 2007 Jun;54(6):578-83
17175028
Arch Intern Med. 2002 May 13;162(9):1021-7
11996612
J Microbiol Immunol Infect. 2004 Jun;37(3):176-84
15221038
Antimicrob Agents Chemother. 1983 Aug;24(2):168-75
6638986
J Bacteriol. 2003 Feb;185(3):788-800
12533454
Chin Med J (Engl). 2007 Jan 20;120(2):136-9
17335656
J Intern Med. 2006 Jun;259(6):606-14
16704562
Clin Infect Dis. 2007 Aug 1;45(3):e25-8
17599300
J Bacteriol. 2009 Jul;191(14):4492-501
19447910
J Infect Dis. 2005 Jul 1;192(1):117-28
15942901
Diagn Microbiol Infect Dis. 2008 Sep;62(1):1-6
18486404
J Infect Dis. 2006 Mar 1;193(5):645-54
16453259
Gut. 2002 Mar;50(3):420-4
11839725
Clin Infect Dis. 1999 Nov;29(5):1360-1
10525011
Clin Microbiol Rev. 1998 Oct;11(4):589-603
9767057
Int J Infect Dis. 2007 Jan;11(1):16-22
16473034
Infect Immun. 2009 Jul;77(7):2657-71
19433545
Clin Infect Dis. 2006 May 15;42(10):1351-8
16619144
Infect Immun. 1989 Feb;57(2):546-52
2643575
J Bacteriol. 1987 Aug;169(8):3414-21
3112120
Res Microbiol. 2000 Apr;151(3):201-8
10865947
Emerg Infect Dis. 2008 Oct;14(10):1592-600
18826824
J Bacteriol. 2008 Jan;190(2):515-26
17981959
J Exp Med. 2004 Mar 1;199(5):697-705
14993253
Travel Med Infect Dis. 2008 Jul;6(4):228-33
18571114
J Clin Microbiol. 2007 Feb;45(2):466-71
17151209
J Clin Microbiol. 2005 Feb;43(2):991-2
15695726
Clin Infect Dis. 1998 Jun;26(6):1434-8
9636876
Hepatol Res. 2005 Jan;31(1):5-6
15652463
Gene. 2004 Aug 4;337:189-98
15276215
J Microbiol Immunol Infect. 2008 Aug;41(4):311-7
18787738
Aust N Z J Med. 1997 Feb;27(1):77-8
9079260
Infect Immun. 2004 Jan;72(1):478-88
14688129
Clin Infect Dis. 2007 Aug 1;45(3):284-93
17599305