Crystallization and preliminary X-ray crystallographic analysis of a GroEL1 fragment from Mycobacterium tuberculosis H37Rv.
Full-length GroEL1 from Mycobacterium tuberculosis H37Rv was cloned, overexpressed and purified. Crystals were obtained by the hanging-drop vapor-diffusion method and contained a 23 kDa GroEL1 fragment. A complete native data set was collected from a single frozen crystal that belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 75.47, b = 78.67, c = 34.89 A, alpha = beta = gamma = 90 degrees , and diffracted to 2.2 A resolution on a home X-ray source.
Sielaff
Bernhard
B
Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston TX 77030, USA.
Lee
Ki Seog
KS
Tsai
Francis T F
FT
D018834:Chaperonin 60 / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*
D018834:Chaperonin 60; D010446:Peptide Fragments
J Bacteriol. 2004 Dec;186(23):8105-13
15547284
Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2608-12
7681982
Nature. 1999 Nov 11;402(6758):147-54
10647006
Methods Enzymol. 1997;276:307-26
27754618
J Mol Biol. 2005 Dec 9;354(4):940-51
16288915
Cell. 2005 Dec 2;123(5):861-73
16325580
Microbiology (Reading). 2002 Oct;148(Pt 10):3129-3138
12368446
Nature. 1997 Aug 21;388(6644):741-50
9285585
J Mol Biol. 1968 Apr 28;33(2):491-7
5700707
Nature. 1994 Oct 13;371(6498):578-86
7935790
J Bacteriol. 1989 Mar;171(3):1379-85
2563997
J Bacteriol. 2005 Feb;187(3):884-9
15659666
FEMS Microbiol Rev. 2009 Jul;33(4):785-800
19416363
FEMS Microbiol Lett. 2005 Aug 1;249(1):87-94
16006064
Nucleic Acids Res. 2009 Aug;37(15):4944-54
19528065