title: Lack of association of Toll-Like Receptor 2 Arg753Gln with cutaneous leishmaniasis.
abstract: The aim of the present study was to investigate the frequency of Arg753Gln and Arg677Trp polymorphisms of TLR2 in patients with cutaneous leishmaniasis (CL) compared to healthy controls. The polymorphisms were genotyped by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system assay (ARMS-PCR). The results showed that the frequency of Arg753Gln genotype was 14.3% and 10.1% in CL patients and normal controls, respectively. No one in either group was homozygous for the mutation. There was no significant difference in the genotype frequency. In contrast to the results for Arg753Gln polymorphism, we did not detect any case with Arg677Trp polymorphism in either control or patient group. In conclusion the TLR2 mutations are found equally in CL patients and healthy subjects.
authors.affiliation: Immunology Department, Pasteur Institute of Iran, Tehran, Islamic Republic of Iran. sohary@yahoo.com
authors.forename: Soheila
authors.initials: S
authors.lastname: Ajdary
authors.forename: Mir-Mostafa
authors.initials: MM
authors.lastname: Ghamilouie
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Alimohammadian
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Hosseini
authors.forename: Saeed-Reza
authors.initials: SR
authors.lastname: Pakzad
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D002648:Child; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007492:Iran; D016773:Leishmaniasis, Cutaneous / Q000235:genetics* / Q000469:parasitology; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D055815:Young Adult
chemical_list: D051195:Toll-Like Receptor 2