title: The bio-functional role of calcium in mesoporous silica xerogels on the responses of osteoblasts in vitro.
abstract: Mesoporous silica xerogels with various amount of calcium (0, 5, 10 and 15%, named m-SXC0, m-SXC5, m-SXC10 and m-SXC15, respectively) were synthesized by template sol-gel methods, and cell responses to m-SXCs were studied using murine pre-osteoblast MC3T3-E1 in vitro. The results showed that cell morphology was not affected by m-SXCs indicating good biocompatibility. Furthermore, cell proliferation ratio on the m-SXCs increased over time, among which m-SXC10 was highest. NO production obviously rose with the increase of Ca content in m-SXCs. ALP activity and PGE(2) level on m-SXC5 significantly improved compared with m-SXC0 while decreased with the increase of Ca content for m-SXC10 and m-SXC15. No obvious discrepancy on osteopontin mRNA expressions was observed among m-SXCs. The collagen I and osteocalcin mRNA expression on m-SXC5 were up-regulated, while decreased on m-SXC15 evidently. The phosphorylation level of ERK 1/2 for the m-SXC10 was highest after 7 days. In conclusion, calcium in m-SXCs plays an important role in osteoblast activity, which indicates mesoporous silica xerogel containing appropriate calcium could stimulate osteoblast proliferation, differentiation, gene expression via the activation of ERK 1/2 signaling pathway, and shows great prospects in bone regeneration field using as a drug controlled release filler.
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, and Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
authors.forename: Huanjun
authors.initials: H
authors.lastname: Zhou
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wu
authors.forename: Jianlin
authors.initials: J
authors.lastname: Shi
authors.forename: Changsheng
authors.initials: C
authors.lastname: Liu
authors.forename: Junfeng
authors.initials: J
authors.lastname: Jia
authors.forename: Chenglong
authors.initials: C
authors.lastname: Dai
authors.forename: Qi
authors.initials: Q
authors.lastname: Gan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation; D066298:In Vitro Techniques; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D012822:Silicon Dioxide / Q000378:metabolism*; D020034:Silicone Gels; D012886:Skull / Q000166:cytology
chemical_list: D020034:Silicone Gels; D015675:Osteocalcin; D053495:Osteopontin; D012822:Silicon Dioxide; D002118:Calcium
references.citation: Biomaterials. 2002 Aug;23(15):3113-22
references.pmid: 12102182
references.citation: J Orthop Res. 2008 Nov;26(11):1440-8
references.pmid: 18404732
references.citation: Biochem Biophys Res Commun. 2000 Sep 24;276(2):461-5
references.pmid: 11027497
references.citation: J Bone Miner Res. 2003 Oct;18(10):1813-24
references.pmid: 14584892
references.citation: J Mater Sci Mater Med. 2007 Nov;18(11):2237-41
references.pmid: 17597361
references.citation: J Bone Miner Res. 1997 Mar;12(3):393-402
references.pmid: 9076582
references.citation: Cell Physiol Biochem. 2003;13(3):135-46
references.pmid: 12876384
references.citation: Biomaterials. 2009 Feb;30(5):743-50
references.pmid: 19027950
references.citation: Biomaterials. 2007 Jan;28(3):393-9
references.pmid: 16997372
references.citation: Clin Orthop Relat Res. 1998 Mar;(348):259-68
references.pmid: 9553560
references.citation: Biomaterials. 2005 Mar;26(9):1043-52
references.pmid: 15369693
references.citation: Endocrinology. 1995 Dec;136(12):5445-53
references.pmid: 7588294
references.citation: Calcif Tissue Int. 2000 Oct;67(4):321-9
references.pmid: 11000347
references.citation: J Mater Sci Mater Med. 2008 Jan;19(1):269-75
references.pmid: 17597360
references.citation: J Biomed Mater Res. 1999 Nov;47(2):176-88
references.pmid: 10449628
references.citation: J Mater Sci Mater Med. 2007 Dec;18(12):2291-6
references.pmid: 17562133
references.citation: Bone. 1999 Mar;24(3):179-85
references.pmid: 10071909
references.citation: Bone. 2011 Apr 1;48(4):894-902
references.pmid: 21147284
references.citation: J Biomed Mater Res. 1998 Jul;41(1):87-94
references.pmid: 9641628
references.citation: Biomaterials. 2001 Jan;22(2):175-85
references.pmid: 11101161
references.citation: J Biomed Mater Res. 2002 Aug;61(2):301-11
references.pmid: 12007211
references.citation: J Biomed Mater Res A. 2003 Jan 1;64(1):189-95
references.pmid: 12483713
references.citation: J Biomed Mater Res A. 2009 Sep 1;90(3):664-70
references.pmid: 18563812
references.citation: J Mater Sci Mater Med. 2009 May;20(5):1131-6
references.pmid: 19089599
references.citation: J Bone Miner Res. 2002 Jul;17(7):1190-9
references.pmid: 12096832
references.citation: Angew Chem Int Ed Engl. 2007;46(40):7548-58
references.pmid: 17854012
references.citation: Langmuir. 2005 Aug 2;21(16):7366-72
references.pmid: 16042467
references.citation: J Biol Chem. 2001 Jun 15;276(24):21351-8
references.pmid: 11292824
references.citation: J Mater Sci Mater Med. 2007 Oct;18(10):2041-4
references.pmid: 17558477
references.citation: Cell Res. 2005 Jan;15(1):11-8
references.pmid: 15686620
references.citation: J Biomed Mater Res B Appl Biomater. 2008 Oct;87(1):114-20
references.pmid: 18437709
references.citation: Blood. 2003 Aug 15;102(4):1405-13
references.pmid: 12702510
references.citation: Drug Deliv. 2008 Nov;15(8):485-92
references.pmid: 18798086
references.citation: J Bone Miner Res. 1995 Mar;10(3):439-46
references.pmid: 7540349
references.citation: Biomacromolecules. 2009 Aug 10;10(8):2067-73
references.pmid: 19621927
references.citation: Int J Mol Med. 2002 Oct;10(4):451-6
references.pmid: 12239592
references.citation: J Pharm Pharmacol. 2007 Oct;59(10):1365-73
references.pmid: 17910811
references.citation: Acta Biomater. 2009 Jan;5(1):193-207
references.pmid: 18755642
references.citation: Injury. 2005 Nov;36 Suppl 3:S20-7
references.pmid: 16188545
references.citation: Int J Artif Organs. 1999 Jul;22(7):516-21
references.pmid: 10493561
references.citation: Biomaterials. 2004 Jun;25(13):2547-55
references.pmid: 14751740
references.citation: Acta Biomater. 2008 Nov;4(6):1894-903
references.pmid: 18583208