title: High-resolution melting analysis of BLU methylation levels in gastric, colorectal, and pancreatic cancers.
abstract: BLU was recently characterized as a novel tumor suppressor gene (TSG), and was epigenetically silenced in some tumor cell lines and primary tumor samples. High-resolution melting (HRM) analysis has been used as a novel tool for analysis of promoter methylation. We used HRM analysis to detect the methylation levels of BLU gene in 100 gastric, 100 colorectal, and 70 pancreatic cancers, and also in an equal number of adjacent normal tissues for all. The frequency of BLU methylation in all three types of cancer was significantly higher than that in normal tissues. And the expression levels of BLU were inversely correlated with methylation levels.
authors.affiliation: Biomedical Research Institute, Shenzhen-PKU-HKUST Medical Center, Shenzhen, Guangdong, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Shao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Guan
authors.forename: Jun
authors.initials: J
authors.lastname: Wan
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
mesh_terms: D000367:Age Factors; D001483:Base Sequence; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D003598:Cytoskeletal Proteins; D019175:DNA Methylation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D009691:Nucleic Acid Denaturation; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D025521:Tumor Suppressor Proteins; C472720:ZMYND10 protein, human