Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions.
Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL(-1) of bFGF on 20 microg mL(-1) of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.
Kamei
Ken-ichiro
K
Department of Molecular & Medical Pharmacology, University of California, Los Angeles, CA 90095, USA. kkamei@mednet.ucla.edu
Ohashi
Minori
M
Gschweng
Eric
E
Ho
Quinn
Q
Suh
Jane
J
Tang
Jinghua
J
For Yu
Zeta Tak
ZT
Clark
Amander T
AT
Pyle
April D
AD
Teitell
Michael A
MA
Lee
Ki-Bum
KB
Witte
Owen N
ON
Tseng
Hsian-Rong
HR
D018929:Cell Culture Techniques / Q000295:instrumentation*; D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D020869:Gene Expression Profiling / Q000295:instrumentation*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D039904:Pluripotent Stem Cells / Q000166:cytology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
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