Transcription regulation in differential expression of the human GSTP1 gene in breast and choriocarcinoma cells.
Glutathione S-transferase P1 is a major phase II detoxification enzyme in most cell types. Aberrant expression of GSTP1 is associated with carcinogenesis and development of multidrug resistance. GSTP1 gene transcription is regulated by promoter methylation and by transcription factors. To elucidate the mechanisms responsible for the different levels of GSTP1 expression observed in Hbl-100 and BeWo cells we utilized truncated promoter constructs to compare the functional role of different promoter elements. We also identified transcription factors binding the responsive elements by electrophoretic mobility shift assay. The applied approaches provided the evidence that binding of transcription factors to ARE, CRE and NF-kappaB sites are responsible for the cell specific levels of GSTP1 expression in Hbl-100 and BeWo cells. It was also indicated that partial promoter methylation occurs in BeWo cells.
Slonchak
A M
AM
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv.
Chwieduk
A
A
Rzeszowska-Wolny
J
J
Yu Obolenska
M
M
D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D002822:Choriocarcinoma / Q000235:genetics*; D019175:DNA Methylation; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014594:Uterine Neoplasms / Q000235:genetics*
D014157:Transcription Factors; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi