title: [Clinical research of HPV DNA detection and HPV RNA detection in single time].
abstract: OBJECTIVE
  To research the application of the single time detection of HPV E6/E7 mRNA and HC2-HPV-DNA in cervical screening project.
  
  
  METHODS
  We detected both HPV E6/E7 mRNA and HC2-HPV-DNA of each sample which collected from 130 cervical disease patients' cervix during Jan. 2008 and July. 2009. TCT results were taken as standard to evaluate the diagnostic accuracy of the above two test methods in detecting high-grade cervical disease.
  
  
  RESULTS
  82.3% (107/130)women were confirmed to infect HPV by HC2-HPV-DNA detection, and 40.0% (52/130) women were confirmed to infect HPV by HPV E6/E7 mRNA detection, there was no significant difference between the two results (chi2 = 24.5, P < 0.05). The sensitivity, specificity, positive predictive value, negative predictive value of HC2-HPV-DNA detection were 90.1%, 22.1%, 37.4% and 82.6%, respectively. The sensitivity, specificity, positive predictive value, negative predictive value of HPV E6/E7 mRNA detection were 65.9%, 73.3%, 55.8% and 80.8%, respectively.
  
  
  CONCLUSIONS
  In clinical cervical screening project of single time, the combination of HC2-HPV-DNA detection and HPV E6/E7 mRNA detection wick take on more potential value than applying each of them alone. RNA;
authors.affiliation: Ningbo Women and Children's Hospital, Zhejiang 315012, China.
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Lu
authors.forename: Zhi-nan
authors.initials: ZN
authors.lastname: Jiang
authors.forename: Shun-mei
authors.initials: SM
authors.lastname: Chen
authors.forename: Shi-rong
authors.initials: SR
authors.lastname: Qin
authors.forename: Lin-jun
authors.initials: LJ
authors.lastname: Zhao
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Yang
authors.forename: Dan-hua
authors.initials: DH
authors.lastname: Shi
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Chen
mesh_terms: D000328:Adult; D052685:Alphapapillomavirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005821:Genetic Techniques; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics; D030361:Papillomavirus Infections / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D014626:Vaginal Smears; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D012367:RNA, Viral