title: Induction of micronuclei and nuclear abnormalities by cyclophosphamide and colchicine in Xenotoca melanosoma (Pisces, Goodeidae) from Lake La Alberca in Michoacan, Mexico.
abstract: This study is a follow-up of previous research in which we described the frequency of spontaneous micronucleated erythrocytes (MNE) in the Goodeid Xenotocoa melanosoma collected from Lake La Alberca, located in the state of Michoacan, Mexico. In the present work, we measured micronuclei (MN) and nuclear abnormalities (NA) in erythrocytes of peripheral blood. Bioassays taken at 24 or 96 hours in either the cyclophosfamide (CP) or colchicine (COL) showed a significant increase in MN and BC (P values ranging from 0.0499 to 0.0036) compared with information from wild organisms collected over 3 years. Concentrationdependent and time-dependent responses support the proposal of using endemic Xenotoca melanosoma as a bioindicator of genotoxicity and cytotoxicity with a high transcendence for the health of the entire ecosystem and evaluation of the Lerma-Chapala watershed.
authors.affiliation: Escuela de Biologia, Universidad Autonoma de Guadalajara, Guadalajara, Mexico. jzavala@uag.mx
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Zavala-Aguirre
authors.forename: Olivia
authors.initials: O
authors.lastname: Torres-Bugarin
authors.forename: Hector Rene
authors.initials: HR
authors.lastname: Buelna-Osben
authors.forename: Lola Paulina
authors.initials: LP
authors.lastname: Flores-Kehn
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Ramos-Ibarra
authors.forename: Guillermo
authors.initials: G
authors.lastname: Zuniga-Gonzalez
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ogura
mesh_terms: D024422:Aneugens / Q000633:toxicity*; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000473:pathology; D003078:Colchicine / Q000633:toxicity*; D003520:Cyclophosphamide / Q000633:toxicity*; D003532:Cyprinodontiformes; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring; D004900:Erythroblasts / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005618:Fresh Water; D008297:Male; D008800:Mexico; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*
chemical_list: D024422:Aneugens; D009153:Mutagens; D003520:Cyclophosphamide; D003078:Colchicine