title: The archaeo-eukaryotic GINS proteins and the archaeal primase catalytic subunit PriS share a common domain.
abstract: Primase and GINS are essential factors for chromosomal DNA replication in eukaryotic and archaeal cells. Here we describe a previously undetected relationship between the C-terminal domain of the catalytic subunit (PriS) of archaeal primase and the B-domains of the archaeo-eukaryotic GINS proteins in the form of a conserved structural domain comprising a three-stranded antiparallel beta-sheet adjacent to an alpha-helix and a two-stranded beta-sheet or hairpin. The presence of a shared domain in archaeal PriS and GINS proteins, the genes for which are often found adjacent on the chromosome, suggests simple mechanisms for the evolution of these proteins.
  
  
  METHODS
  This article was reviewed by Zvi Kelman (nominated by Michael Galperin) and Kira Makarova.
authors.affiliation: Centre for Biomolecular Sciences, School of Biology, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK. as2326@st-andrews.ac.uk
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Swiatek
authors.forename: Stuart A
authors.initials: SA
authors.lastname: Macneill
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics; D020134:Catalytic Domain; D019915:DNA Primase / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D019843:Archaeal Proteins; D011506:Proteins; D019915:DNA Primase
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