title: Interaction between non-anionic phospholipids and cytochrome c induced by reactive oxygen species.
abstract: The oxidative interaction of cytochrome c (Cyt c) with liposomes of Palmitoyl Linoleyl Phosphatidyl Choline (PLPC) initiated by radio-induced free radicals was investigated. Results showed that the peroxidation of PLPC is decreased in the presence of Cyt c, meaning that this latter is the preferential target of hydroxyl radicals. In addition, when Cyt c was incubated with peroxidized PLPC, it was found to be able to decompose hydroperoxides of PLPC into hydroxides. The peroxidase activity of Cyt c proceeded via the opening of the tertiary structure of Cyt c, as suggested by the loss of the sixth coordination bond of the heme-iron. Even if it is known to preferentially interact with cardiolipin, this work shows that Cyt c is also able to interact with hydroperoxide species of non-anionic phospholipids.
authors.affiliation: Equipe de Physico-Chimie des Espèces Radicalaires, CNRS UMR 8601, Université Paris Descartes, 45 rue des Saints-Pères, 75006 Paris, France.
authors.forename: Nazha
authors.initials: N
authors.lastname: Sidahmed-Adrar
authors.forename: Catherine
authors.initials: C
authors.lastname: Marchetti
authors.forename: Dominique
authors.initials: D
authors.lastname: Bonnefont-Rousselot
authors.forename: Juliette
authors.initials: J
authors.lastname: Thariat
authors.forename: Delphine
authors.initials: D
authors.lastname: Onidas
authors.forename: Daniel
authors.initials: D
authors.lastname: Jore
authors.forename: Monique
authors.initials: M
authors.lastname: Gardes-Albert
authors.forename: Fabrice
authors.initials: F
authors.lastname: Collin
mesh_terms: D002308:Cardiolipins / Q000737:chemistry; D045304:Cytochromes c / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry*
chemical_list: D002308:Cardiolipins; D008081:Liposomes; D010713:Phosphatidylcholines; D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical; C055402:1-palmitoyl-2-linoleoylphosphatidylcholine; D045304:Cytochromes c