Investigating the role of P311 in the hypertrophic scar.
The mechanisms of hypertrophic scar formation are not fully understood. We previously screened the differentially expressed genes of human hypertrophic scar tissue and identified P311 gene as upregulated. As the activities of P311 in human fibroblast function are unknown, we examined the distribution of it and the effects of forced expression or silencing of expression of P311. P311 expression was detected in fibroblast-like cells from the hypertrophic scar of burn injury patients but not in peripheral blood mononuclear cells, bone marrow mesenchymal stem cells, epidermal cells or normal skin dermal cells. Transfection of fibroblasts with P311 gene stimulated the expression of alpha-smooth muscle actin (alpha-SMA), TGF-beta1 and alpha1(I) collagen (COL1A1), and enhanced the contraction of fibroblast populated collagen lattices (FPCL). In contrast, interference of fibroblast P311 gene expression decreased the TGF-beta1 mRNA expression and reduced the contraction of fibroblasts in FPCL. These results suggest that P311 may be involved in the pathogenesis of hypertrophic scar via induction of a myofibroblastic phenotype and of functions such as TGF-beta1 expression. P311 could be a novel target for the control of hypertrophic scar development.
Tan
Jianglin
J
State Key Laboratory for Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
Peng
Xu
X
Luo
Gaoxing
G
Ma
Bing
B
Cao
Chuan
C
He
Weifeng
W
Yuan
Shunzong
S
Li
Shirong
S
Wilkins
John A
JA
Wu
Jun
J
D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D002056:Burns; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000209:etiology* / Q000473:pathology; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics; D000090602:Collagen Type I, alpha 1 Chain; D005347:Fibroblasts / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D015513:Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis / Q000235:genetics
C541116:ACTA2 protein, human; D000199:Actins; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; C428027:NREP protein, human; D009419:Nerve Tissue Proteins; D015513:Oncogene Proteins; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1
Am J Dermatopathol. 2004 Oct;26(5):379-84
15365369
J Neurochem. 2004 Nov;91(3):737-44
15485502
Biochem Biophys Res Commun. 2009 May 1;382(2):259-63
19285964
Experientia. 1971 May 15;27(5):549-50
5132594
J Biol Chem. 2000 Feb 11;275(6):4215-9
10660586
J Interferon Cytokine Res. 2007 Nov;27(11):921-30
18052725
J Invest Dermatol. 2008 Oct;128(10):2518-25
18401422
J Cell Biol. 1993 Jul;122(1):103-11
8314838
J Burn Care Rehabil. 1995 Sep-Oct;16(5):535-8
8537427
Science. 1997 Apr 4;276(5309):75-81
9082989
Genes Brain Behav. 2008 Oct;7(7):786-95
18616608
J Invest Dermatol. 2008 Feb;128(2):280-91
17671518
J Cell Biol. 1994 Feb;124(4):401-4
8106541
J Trauma. 2003 Jun;54(6):1212-24
12813346
J Trauma. 2004 Dec;57(6):1276-86
15625461
Br J Dermatol. 2008 Apr;158(4):713-20
18284397
J Pathol. 2008 Jan;214(2):199-210
18161745
N Engl J Med. 1999 Sep 2;341(10):738-46
10471461
Br J Dermatol. 2009 Jul;161(1):8-18
19508304
J Clin Invest. 2002 Nov;110(9):1349-58
12417574
Dermatol Surg. 1999 Aug;25(8):631-8
10491047
Burns. 2009 Feb;35(1):15-29
18952381
J Burn Care Res. 2008 Mar-Apr;29(2):363-8
18354295
Indian J Dermatol Venereol Leprol. 2005 Jan-Feb;71(1):3-8
16394352
Plast Reconstr Surg. 2004 Feb;113(2):633-40
14758226