Inducement of G-quadruplex DNA forming and down-regulation of oncogene c-myc by bile acid-amino acid conjugate-BAA.
Human c-myc gene is a central regulator of cellular proliferation and cell growth, and G-quadruplexes have been proven to be the transcriptional controller of this gene. In this study, the interaction of bile acid-amino acid conjugate (BAA) with G-quadruplexes in c-myc was investigated by circular dichroism spectroscopy, nuclear magnetic resonance (NMR) measurement, and quantitative real-time polymerase chain reaction (PCR) assay. The experimental results indicated that BAA has the ability to selectively induce the formation of parallel G-quadruplexes in c-myc, which leads to down-regulation of c-myc transcription in the human breast cancer cell MCF-7.
Tian
Mingyue
M
Beijing Key Lab of Gene Engineering Drugs & Biological Technology, College of Life Sciences, Beijing Normal University, Beijing, P. R. China.
Zhang
Xiufeng
X
Li
Yan
Y
Ju
Yong
Y
Xiang
Junfeng
J
Zhao
Changqi
C
Tang
Yalin
Y
D000596:Amino Acids / Q000737:chemistry / Q000494:pharmacology*; D001647:Bile Acids and Salts / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry* / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects* / Q000235:genetics; D054856:G-Quadruplexes / Q000187:drug effects*; D006801:Humans; D008968:Molecular Conformation; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D001647:Bile Acids and Salts; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D004247:DNA