title: Development of an indirect ELISA for the detection of duck circovirus infection in duck flocks.
abstract: Infection with duck circovirus (DuCV) is associated with growth retardation and developmental problems in farmed ducks. To detect DuCV-specific antibody in duck serum, an indirect enzyme-linked immunosorbent assay (iELISA) method was developed using the recombinant capsid protein antigen prepared by cloning the cap (Cap) gene of DuCV FJ0601 strain into pET-32a (+) vector and expressed in Escherichia coli. Using the optimized iELISA method, DuCV-specific antibodies were detected in 157 (12.96%) of 1211 samples obtained from 17 (89.47%) of 19 meat duck flocks aged from 25 to 40 days and in 89 (22.08%) of 403 samples obtained from 9 (75%) of 12 breeder flocks aged from 14 to 61 weeks. These results indicated that the iELISA method is useful for serological diagnosis of DuCV infection and epidemiological investigation.
authors.affiliation: Laboratory of Taishan Scholar Immunobiology, China.
authors.forename: Shao-Ning
authors.initials: SN
authors.lastname: Liu
authors.forename: Xing-Xiao
authors.initials: XX
authors.lastname: Zhang
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Zou
authors.forename: Zhi-Jing
authors.initials: ZJ
authors.lastname: Xie
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Zhu
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhao
authors.forename: En-min
authors.initials: EM
authors.lastname: Zhou
authors.forename: Shi-Jin
authors.initials: SJ
authors.lastname: Jiang
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D036022:Capsid Proteins / Q000276:immunology; D018173:Circoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus; D004372:Ducks / Q000821:virology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D011994:Recombinant Proteins / Q000276:immunology; D014760:Viral Fusion Proteins / Q000276:immunology
chemical_list: D036022:Capsid Proteins; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins