Determination of lamotrigine in human serum by high-performance liquid chromatography–tandem mass spectrometry.
A rapid and sensitive liquid chromatography–tandem mass spectrometry method was developed and validated for quantification of lamotrigine in human serum. After a simple protein precipitation using acetonitrile, the analytes were separated on a Shideido 150 mm × 2.0 mm, 5 μm Capcell Pak C18 MG column using 70% acetonitrileas mobile phase at a flow rate of 200 μl/min. Lamotrigine was eluted at 1.98 min, ionized using electrospray ionization source, and then detected by multiple reaction monitoring mode. The precursor to product ion transitions of m/z 256.1–109.0 was used to quantify. The analytical measurement range is from 0.1 to 20 μg/ml and the upper clinical reportable range is chosen to be 100 μg/ml. The method was successfully employed in a clinical application.
Lee
Woonhyoung
W
Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea.
Kim
Jeong-Ho
JH
Kim
Hyon-Suk
HS
Kwon
Oh Hun
OH
Lee
Byung In
BI
Heo
Kyoung
K
D000927:Anticonvulsants / Q000097:blood / Q000493:pharmacokinetics; D018692:Antimanic Agents / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016903:Drug Monitoring / Q000379:methods; D004827:Epilepsy / Q000188:drug therapy; D006801:Humans; D000077213:Lamotrigine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014227:Triazines / Q000097:blood* / Q000493:pharmacokinetics
D000927:Anticonvulsants; D018692:Antimanic Agents; D014227:Triazines; D000077213:Lamotrigine
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