Determinants of Rad21 localization at the centrosome in human cells.
Cohesin proteins help maintain the physical associations between sister chromatids that arise in S-phase and are removed in anaphase. Recent studies found that cohesins also localize to the centrosomes, the organelles that organize the mitotic bipolar spindle. We find that the cohesin protein Rad21 localizes to centrosomes in a manner that is dependent upon known regulators of sister chromatid cohesion as well as regulators of centrosome function. These data suggest that Rad21 functions at the centrosome and that the regulators of Rad21 coordinate the centrosome and chromosomal functions of cohesin.
Giménez-Abián
Juan F
JF
Department of Genetics, Cell Biology & Development, University of Minnesota Medical School, Minneapolis, MN, USA.
Díaz-Martínez
Laura A
LA
Beauchene
Nicole A
NA
Hsu
Wei-Shan
WS
Tsai
Hung-Ji
HJ
Clarke
Duncan J
DJ
D000705:Anaphase; D064127:Aurora Kinases; D018797:Cell Cycle Proteins / Q000032:analysis / Q000378:metabolism; D018385:Centrosome / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000378:metabolism; D020090:Chromosome Segregation; D004268:DNA-Binding Proteins; D010450:Endopeptidases / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D009687:Nuclear Proteins / Q000032:analysis* / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D064247:Separase; D000097722:Cohesins; D000097869:Polo-Like Kinase 1
D064127:Aurora Kinases; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D010450:Endopeptidases; D009687:Nuclear Proteins; D010750:Phosphoproteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D064247:Separase; D000097722:Cohesins; D000097869:Polo-Like Kinase 1; C429022:RAD21 protein, human