Preclinical Alzheimer disease: brain oxidative stress, Abeta peptide and proteomics.
Alzheimer disease (AD) is a neurodegenerative disorder characterized clinically by progressive memory loss and subsequent dementia and neuropathologically by senile plaques, neurofibrillary tangles, and synapse loss. Interestingly, a small percentage of individuals with normal antemortem psychometric scores meet the neuropathological criteria for AD (termed 'preclinical' AD (PCAD)). In this study, inferior parietal lobule (IPL) from PCAD and control subjects was compared for oxidative stress markers by immunochemistry, amyloid beta peptide by ELISA, and identification of protein expression differences by proteomics. We observed a significant increase in highly insoluble monomeric Abeta42, but no significant differences in oligomeric Abeta nor in oxidative stress measurements between controls and PCAD subjects. Expression proteomics identified proteins whose trends in PCAD are indicative of cellular protection, possibly correlating with previous studies showing no cell loss in PCAD. Our analyses may reveal processes involved in a period of protection from neurodegeneration that mimic the clinical phenotype of PCAD.
Aluise
Christopher D
CD
Department of Chemistry, Center of Membrane Sciences, and Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40506, USA.
Robinson
Renã A Sowell
RA
Beckett
Tina L
TL
Murphy
M Paul
MP
Cai
Jian
J
Pierce
William M
WM
Markesbery
William R
WR
Butterfield
D Allan
DA
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000175:diagnosis / Q000378:metabolism* / Q000503:physiopathology*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D010296:Parietal Lobe / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D010446:Peptide Fragments / Q000378:metabolism*; D050050:Protein Carbonylation; D040901:Proteomics / Q000379:methods*; D013057:Spectrum Analysis / Q000379:methods; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42); C002744:3-nitrotyrosine; D014443:Tyrosine
Cell Death Differ. 2000 Aug;7(8):747-53
10918449
Trends Mol Med. 2001 Dec;7(12):548-54
11733217
Acta Neuropathol. 2009 Jul;118(1):131-50
19288120
Science. 1992 Oct 2;258(5079):126-9
1439760
Neurology. 2009 Sep 1;73(9):665-73
19587326
Neuron. 1995 Mar;14(3):671-80
7695914
Lab Invest. 1991 Mar;64(3):352-7
1706004
Biochemistry. 1998 Apr 21;37(16):5566-75
9548941
Arch Neurol. 2007 Jul;64(7):954-6
17620484
Brain Res. 1991 Nov 1;563(1-2):311-4
1786545
Anal Biochem. 2004 Dec 1;335(1):81-90
15519574
Neurology. 1999 Mar 23;52(5):1090-3
10102440
Neurobiol Dis. 2006 May;22(2):223-32
16466929
Bol Estud Med Biol. 1996 Jan-Dec;44(1-4):5-10
9369031
Arch Neurol. 1999 Dec;56(12):1449-52
10593298
Biochemistry. 1995 Oct 31;34(43):14185-92
7578016
Neuron. 2005 Jul 21;47(2):191-199
16039562
Neurobiol Aging. 2009 Jul;30(7):1026-36
19376612
J Alzheimers Dis. 2007 Aug;12(1):61-72
17851195
Brain Pathol. 2010 Mar;20(2):343-50
19371353
Neurobiol Dis. 2008 Apr;30(1):107-20
18325775
Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):14163-8
17715058
Nature. 1996 Apr 11;380(6574):544-7
8606777
Annu Rev Neurosci. 2001;24:1121-59
11520930
J Neurosci Res. 2007 Nov 1;85(14):3036-40
17510979
Free Radic Biol Med. 2010 Jan 1;48(1):136-44
19854267
J Neurochem. 1994 Dec;63(6):2179-84
7964738
Biochim Biophys Acta. 2008 Oct;1782(10):549-58
18760351
Free Radic Biol Med. 2010 Jun 15;48(12):1570-6
20171275
Nature. 1992 Sep 24;359(6393):325-7
1406936
Brain Res. 2007 May 7;1148:243-8
17395167
Genes Dev. 1998 Mar 1;12(5):706-20
9499405
J Mol Neurosci. 1998 Oct;11(2):151-64
10096042
Am J Pathol. 1998 Nov;153(5):1551-60
9811347
Neurochem Res. 2008 Dec;33(12):2540-6
18320305
Neurobiol Dis. 2007 Sep;27(3):301-11
17651976
J Geriatr Psychiatry Neurol. 1994 Jul-Sep;7(3):153-8
7522458
Nature. 2006 Mar 23;440(7083):528-34
16554819
Neurobiol Aging. 2006 Jul;27(7):918-25
15950321
J Neurochem. 2007 Jun;101(5):1172-84
17286590
Proc Natl Acad Sci U S A. 1990 May;87(10):3861-5
1692625
J Biol Chem. 1996 Nov 1;271(44):27241-4
8910296
Nat Med. 2008 Aug;14(8):837-42
18568035
Nat Rev Mol Cell Biol. 2007 Feb;8(2):101-12
17245412
Exp Neurol. 1995 Jun;133(2):105-12
7649217
Ann Neurol. 2005 Nov;58(5):730-5
16240347
Neurol Res. 2006 Mar;28(2):155-63
16551433
J Cell Mol Med. 2009 Aug;13(8B):2019-2029
18752637
J Cell Biol. 1998 May 4;141(3):727-39
9566972
Antioxid Redox Signal. 2010 Mar;12(3):327-36
19686046
J Alzheimers Dis. 2007 May;11(2):153-64
17522440
Eur J Biochem. 1994 Sep 1;224(2):265-71
7523115
J Clin Invest. 2008 May;118(5):1877-89
18431510
J Neurosci Res. 2009 Feb;87(2):408-17
18803298
Arch Neurol. 2009 May;66(5):632-7
19433663
J Immunol. 1999 Jan 15;162(2):799-806
9916701
Expert Rev Proteomics. 2008 Apr;5(2):157-60
18466047
Cell Death Differ. 2002 Jun;9(6):661-70
12032675
J Neurosci. 2007 Mar 14;27(11):2866-75
17360908
Neurobiol Aging. 2008 Jan;29(1):51-70
17055614
Expert Opin Ther Targets. 2002 Aug;6(4):461-8
16353421
Neurobiol Aging. 2004 Oct;25(9):1205-12
15312966
Methods Enzymol. 2006;417:256-73
17132510
J Neurochem. 1992 Aug;59(2):776-9
1321237
Nature. 1992 Sep 24;359(6393):322-5
1383826
J Biol Chem. 1996 Dec 13;271(50):31885-93
8943232
Nat Med. 1998 Jul;4(7):827-31
9662375
J Cell Mol Med. 2007 Jul-Aug;11(4):839-51
17760844
J Biol Chem. 2009 Jun 19;284(25):17253-17265
19380581
J Neurosci. 2009 Aug 26;29(34):10582-7
19710311
J Alzheimers Dis. 2009;18(3):665-75
19661626
J Neurosci. 2003 Sep 17;23(24):8532-8
13679422
J Biol Chem. 2002 May 10;277(19):16599-605
11867637
Histol Histopathol. 2008 Jan;23(1):67-76
17952859
Neuron. 2009 Jun 25;62(6):788-801
19555648