title: Osteoinduction in umbilical cord- and palate periosteum-derived mesenchymal stem cells.
abstract: Adult abdominoplasty (AA) fat is an ideal source for mesenchymal stem cells (MSCs) because it is discarded after surgery, abundant, and easy to harvest. Children however, do not have the same abundant quantities of fat as adults, nor are they likely to undergo a procedure during which fat is routinely discarded. Hence, finding an alternate source for MSCs in children is a reasonable strategy. Two such sources are the palate periosteum (PP) and the umbilical cord (UC). Advantages for PP as a source of MSCs are accessibility during palate repair, ease of harvest, and minimal risk to the patient. The UC, like AA, is a discarded tissue, with a theoretically unlimited supply, which can be harvested in children with craniofacial bone abnormalities in advance of reconstructive procedures. Our objective in this study is to characterize MSCs from 3 sources (AA, PP, and UC) by surface marker prevalence, and to assess osteoinductive capability. Institutional review board approval was obtained for harvest of AA, PP, and UC. The presence of MSCs was determined using immunostaining and flow cytometry for cell surface markers CD73, CD90, CD105, and SSEA-4. Osteogenesis was induced using osteogenic medium. Osteoinduction was evaluated using Alizarin red staining, and real-time polymerase chain reaction for bone morphogenetic protein-2, alkaline phosphatase, and osteocalcin at 7, 14, and 21 days. MSCs from AA, PP, and UC all stained positive for CD73, CD90, CD105, and SSEA-4. Flow cytometry demonstrated significant differences in expression of CD90 and SSEA-4 but similar values for CD73 and CD105. Following osteoinduction, MSCs from all sources stained positive for calcium deposition. In UC MSCs, reverse transcriptase-polymerase chain reaction demonstrated greater elevation in bone morphogenetic protein-2 and alkaline phosphatase mRNA beginning at day 7 and extending to day 21. Osteocalcin mRNA levels were comparable for all 3 sources of MSCs. For children with craniofacial bone defects, UC-derived MSCs may be ideal for tissue engineered bone: temporally, the UC can be harvested in advance of surgical timing for the need for bone, is readily available, easy to harvest, and leads to osteoinduction that is more robust than either AA or PP.
authors.affiliation: Division of Plastic Surgery, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Caballero
authors.forename: Courtney R
authors.initials: CR
authors.lastname: Reed
authors.forename: Gitanjali
authors.initials: G
authors.lastname: Madan
authors.forename: John A
authors.initials: JA
authors.lastname: van Aalst
mesh_terms: D015415:Biomarkers / Q000032:analysis; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D010159:Palate / Q000166:cytology*; D010521:Periosteum / Q000166:cytology*; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling; D050507:Subcutaneous Fat, Abdominal / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D015415:Biomarkers