Nucleophosmin/B23 inhibits Eg5-mediated microtubule depolymerization by inactivating its ATPase activity.
Nucleophosmin/B23, an abundant nucleolar protein, plays multiple roles in cell growth and proliferation, and yet, little has been studied about its function in regulating dynamics of microtubules. Here, we report that B23 directly interacts with Eg5, a member of the kinesin family, in the cytosol. The DNA/RNA binding domain of B23 and the motor domain of Eg5 were found to be involved in their interaction. Both in vivo and in vitro evidences showed that B23 acts as an upstream regulator of Eg5 in promoting microtubule polymerization. Moreover, we further demonstrated that B23 regulates microtubule dynamics by directly inhibiting Eg5 ATPase activity.
Wang
Guoxing
G
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Gao
Xiang
X
Huang
Yun
Y
Yao
Zhan
Z
Shi
Qinghua
Q
Wu
Mian
M
D000251:Adenosine Triphosphatases / Q000737:chemistry; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D034741:RNA, Small Interfering / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D013552:Swine; D020798:Two-Hybrid System Techniques
C470243:KIF11 protein, human; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D000090243:Nucleophosmin; D000251:Adenosine Triphosphatases; D016547:Kinesins
Cancer Res. 2006 Feb 15;66(4):2059-66
16489005
Curr Biol. 2002 Jan 8;12(1):1-11
11790298
Biosci Biotechnol Biochem. 2002 Oct;66(10):2239-42
12450141
EMBO J. 2007 Feb 21;26(4):1068-80
17290218
Science. 2007 Apr 6;316(5821):120-3
17412962
Nat Cell Biol. 2005 Aug;7(8):823-30
16041368
J Biol Chem. 2001 Jun 15;276(24):21529-37
11278991
Cell. 2000 Sep 29;103(1):127-40
11051553
Cell. 2009 Jan 9;136(1):110-22
19135893
Cold Spring Harb Symp Quant Biol. 1982;46 Pt 1:483-93
6955089
J Cell Biochem. 2003 Dec 15;90(6):1140-8
14635188
Trends Biochem Sci. 1996 Jun;21(6):197-8
8744350
Blood. 2003 Jan 1;101(1):289-91
12393441
Int J Cancer. 1999 Jun 11;81(6):923-9
10362140
Cell Cycle. 2005 Nov;4(11):1593-8
16205118
Cell. 1999 Jan 8;96(1):69-78
9989498
Mol Cell Biol. 2005 Feb;25(4):1258-71
15684379
Nat Rev Cancer. 2006 Jul;6(7):493-505
16794633
Nat Cell Biol. 2006 Sep;8(9):957-62
16906145
J Biol Chem. 1991 Feb 25;266(6):3987-94
1671676
Cell. 2008 Nov 28;135(5):894-906
19041752
J Biol Chem. 2009 Oct 9;284(41):28410-28419
19661056
J Cell Biol. 2007 Sep 10;178(6):1081-91
17846176
Biochem Biophys Res Commun. 1999 Apr 21;257(3):865-70
10208875
J Biol Chem. 2009 Nov 13;284(46):31992-2001
19759393
Dev Cell. 2009 Oct;17(4):437-8
19853556
Oncogene. 2002 Sep 9;21(40):6170-4
12214246
J Cell Biol. 1988 Oct;107(4):1437-48
3170635
Blood. 2005 Aug 1;106(3):899-902
15831697
Nature. 2005 Oct 13;437(7061):1038-42
16222248
Cell. 1996 Jun 28;85(7):943-6
8674121
Biophys J. 1997 Mar;72(3):1357-75
9138581
Cell. 1989 Feb 10;56(3):379-90
2914325