Analysis of arsenical metabolites in biological samples.
Quantitation of iAs and its methylated metabolites in biological samples provides dosimetric information needed to understand dose-response relations. Here, methods are described for separation of inorganic and mono-, di-, and trimethylated arsenicals by thin layer chromatography. This method has been extensively used to track the metabolism of the radionuclide [(73)As] in a variety of in vitro assay systems. In addition, a hydride generation-cryotrapping-gas chromatography-atomic absorption spectrometric method is described for the quantitation of arsenicals in biological samples. This method uses pH-selective hydride generation to differentiate among arsenicals containing trivalent or pentavalent arsenic.
Hernandez-Zavala
Araceli
A
Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7310, USA.
Drobna
Zuzana
Z
Styblo
Miroslav
M
Thomas
David J
DJ
D001151:Arsenic / Q000378:metabolism*; D001152:Arsenicals / Q000032:analysis*; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D010084:Oxidation-Reduction; D011868:Radioisotopes / Q000378:metabolism; D013048:Specimen Handling; D013054:Spectrophotometry, Atomic / Q000295:instrumentation / Q000379:methods
D001152:Arsenicals; D011868:Radioisotopes; D001151:Arsenic
Chem Res Toxicol. 2004 Dec;17(12):1621-9
15606138
Anal Biochem. 1977 Apr;78(2):557-60
851228
Toxicol Appl Pharmacol. 2001 Aug 1;174(3):282-93
11485389
Spectrochim Acta Part B At Spectrosc. 2008 Mar;63(3):396-406
18521190
J Chromatogr B Biomed Appl. 1995 Jun 9;668(1):21-9
7550978
Environ Health Perspect. 1977 Aug;19:147-50
908293
J Chromatogr B Biomed Appl. 1996 Feb 23;677(1):161-6
8925090
Clin Chem. 2000 Nov;46(11):1781-6
11067813
J Anal At Spectrom. 2008;23:342-351
18677417
Science. 1973 Dec 21;182(4118):1247-9
4800529
Arch Toxicol. 1985 Jun;57(2):125-9
4026571