Cell signalling by microRNA165/6 directs gene dose-dependent root cell fate.
A key question in developmental biology is how cells exchange positional information for proper patterning during organ development. In plant roots the radial tissue organization is highly conserved with a central vascular cylinder in which two water conducting cell types, protoxylem and metaxylem, are patterned centripetally. We show that this patterning occurs through crosstalk between the vascular cylinder and the surrounding endodermis mediated by cell-to-cell movement of a transcription factor in one direction and microRNAs in the other. SHORT ROOT, produced in the vascular cylinder, moves into the endodermis to activate SCARECROW. Together these transcription factors activate MIR165a and MIR166b. Endodermally produced microRNA165/6 then acts to degrade its target mRNAs encoding class III homeodomain-leucine zipper transcription factors in the endodermis and stele periphery. The resulting differential distribution of target mRNA in the vascular cylinder determines xylem cell types in a dosage-dependent manner.
Carlsbecker
Annelie
A
Institute of Biotechnology/Department of Biosciences, University of Helsinki, FIN-00014, Finland.
Lee
Ji-Young
JY
Roberts
Christina J
CJ
Dettmer
Jan
J
Lehesranta
Satu
S
Zhou
Jing
J
Lindgren
Ove
O
Moreno-Risueno
Miguel A
MA
Vatén
Anne
A
Thitamadee
Siripong
S
Campilho
Ana
A
Sebastian
Jose
J
Bowman
John L
JL
Helariutta
Ykä
Y
Benfey
Philip N
PN
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D019521:Body Patterning; D019070:Cell Lineage; D002465:Cell Movement; D004707:Endoderm / Q000166:cytology / Q000378:metabolism; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D038081:Organogenesis; D018517:Plant Roots / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D034443:RNA Transport; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism; D052584:Xylem / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
D029681:Arabidopsis Proteins; D035683:MicroRNAs; D018749:RNA, Plant; C100978:SCR protein, Arabidopsis; C410510:SHORT ROOT protein, Arabidopsis; D014157:Transcription Factors
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