The mitochondrial genome of the pathogenic yeast Candida subhashii: GC-rich linear DNA with a protein covalently attached to the 5' termini.
As a part of our initiative aimed at a large-scale comparative analysis of fungal mitochondrial genomes, we determined the complete DNA sequence of the mitochondrial genome of the yeast Candida subhashii and found that it exhibits a number of peculiar features. First, the mitochondrial genome is represented by linear dsDNA molecules of uniform length (29 795 bp), with an unusually high content of guanine and cytosine residues (52.7 %). Second, the coding sequences lack introns; thus, the genome has a relatively compact organization. Third, the termini of the linear molecules consist of long inverted repeats and seem to contain a protein covalently bound to terminal nucleotides at the 5' ends. This architecture resembles the telomeres in a number of linear viral and plasmid DNA genomes classified as invertrons, in which the terminal proteins serve as specific primers for the initiation of DNA synthesis. Finally, although the mitochondrial genome of C. subhashii contains essentially the same set of genes as other closely related pathogenic Candida species, we identified additional ORFs encoding two homologues of the family B protein-priming DNA polymerases and an unknown protein. The terminal structures and the genes for DNA polymerases are reminiscent of linear mitochondrial plasmids, indicating that this genome architecture might have emerged from fortuitous recombination between an ancestral, presumably circular, mitochondrial genome and an invertron-like element.
Fricova
Dominika
D
Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 842 15 Bratislava, Slovak Republic.
Valach
Matus
M
Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 842 15 Bratislava, Slovak Republic.
Farkas
Zoltan
Z
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Kozep fasor 52, H-6726 Szeged, Hungary.
Pfeiffer
Ilona
I
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Kozep fasor 52, H-6726 Szeged, Hungary.
Kucsera
Judit
J
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Kozep fasor 52, H-6726 Szeged, Hungary.
Tomaska
Lubomir
L
Department of Genetics, Comenius University, Faculty of Natural Sciences, Mlynska dolina B-1, 842 15 Bratislava, Slovak Republic.
Nosek
Jozef
J
Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 842 15 Bratislava, Slovak Republic.
D000595:Amino Acid Sequence; D002175:Candida / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D002177:Candidiasis / Q000382:microbiology; D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020862:GC Rich Sequence; D054629:Genome, Mitochondrial; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D011485:Protein Binding; D016415:Sequence Alignment
D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins
Annu Rev Biochem. 1991;60:39-71
1883199
J Bacteriol. 2001 Feb;183(3):865-72
11208783
FEBS Lett. 1998 Dec 4;440(3):325-31
9872396
BMC Evol Biol. 2006 Nov 22;6:99
17121679
Nucleic Acids Res. 2006;34(20):6051-63
17071961
EMBO J. 1987 Dec 20;6(13):4197-203
3327690
Gene. 2006 Oct 15;381:92-101
16945488
Syst Biol. 2003 Oct;52(5):696-704
14530136
J Biol Chem. 2005 Mar 18;280(11):10840-5
15657051
Gene. 2005 Mar 28;348:89-99
15777661
Yeast. 1996 Mar 15;12(3):241-6
8904336
Mol Gen Genet. 1995 Apr 10;247(1):61-72
7715605
Mol Biol Evol. 2002 Mar;19(3):310-9
11861890
Curr Genet. 2008 Aug;54(2):105-9
18568348
Gene. 2008 Feb 29;410(1):177-86
18222615
BMC Genomics. 2008 Jul 26;9:350
18655725
Microbiol Rev. 1990 Mar;54(1):66-74
2157134
Mol Cell Biol. 1993 Apr;13(4):2309-14
8455612
Nucleic Acids Res. 1997 Mar 1;25(5):955-64
9023104
Yeast. 1999 Jun 30;15(9):781-91
10398346
Nucleic Acids Res. 1982 Sep 11;10(17):5303-18
7145702
Curr Genet. 2003 Nov;44(2):73-84
12898180
Curr Genet. 1996 Feb;29(3):257-64
8595672
J Theor Biol. 1973 Sep 14;41(1):181-90
4754905
J Bacteriol. 1994 Sep;176(18):5622-30
7521869
Mitochondrion. 2008 Jan;8(1):15-25
18326073
Med Mycol. 2009 May;47(3):305-11
18798116
Nucleic Acids Res. 1998 May 15;26(10):2286-90
9580676
Curr Genet. 1994 Nov-Dec;26(5-6):506-11
7533058
Mol Genet Genomics. 2004 Sep;272(2):173-80
15449175
Mol Cell Biol. 1993 Apr;13(4):2315-23
8455613
Nat Struct Mol Biol. 2009 Oct;16(10):1010-5
19809492
J Mol Biol. 2004 Apr 2;337(4):829-41
15033354
EMBO J. 1991 Dec;10(12):3923-9
1935911
Trends Genet. 1998 May;14(5):184-8
9613202
Curr Genet. 1991 Aug;20(3):225-37
1934129
Mol Biol Evol. 2008 Mar;25(3):487-96
18222946
Nat New Biol. 1972 Oct 18;239(94):197-201
4507727
Curr Genet. 2000 Dec;38(5):283-90
11191213
Curr Genet. 1992 Dec;22(6):491-500
1473181
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
15034147
Nucleic Acids Res. 2000 Nov 15;28(22):4479-87
11071936
Plant Mol Biol. 2009 Dec;71(6):627-39
19774466