title: [Genetically encoded FRET-pair on the basis of terbium-binding peptide and red fluorescent protein].
abstract: The genetically encoded FRET-pair was developed on the basis of terbium-binding peptide and red fluorescent protein DsRed2. To study fluorescence resonance energy transfer within FRET-pair, the gene-engineered construction was obtained, where sequences of terbium-binding peptide and red fluorescent protein DsRed2 were fused in single reading frame. The expression of this construction in strain E. coli BL21 (DE3) was studied and conditions of synthesis, isolation and purification of recombinant protein were optimized. The hydrodynamic radius of hybrid protein was determined by the method of dynamic light scattering. Energy transfer between sensitized terbium and red fluorescent protein was confirmed by the methods of phosporescent spectroscopy. The obtained FRET-pair can be used both for studies in vitro and as a reporter in living cells.
authors.forename: L R
authors.initials: LR
authors.lastname: Arslanbaeva
authors.forename: V V
authors.initials: VV
authors.lastname: Zherdeva
authors.forename: T V
authors.initials: TV
authors.lastname: Ivashina
authors.forename: L M
authors.initials: LM
authors.lastname: Vinokurov
authors.forename: A L
authors.initials: AL
authors.lastname: Rusanov
authors.forename: A P
authors.initials: AP
authors.lastname: Savitskiĭ
mesh_terms: D004735:Energy Transfer; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D010957:Plasmids; D011485:Protein Binding; D011487:Protein Conformation; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013725:Terbium / Q000737:chemistry / Q000378:metabolism*; D000097573:Red Fluorescent Protein
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D013725:Terbium; D000097573:Red Fluorescent Protein