Expression of Sox 9 and type II and X collagens in regenerated condyle.
The present study was designed to examine the expression of Sox 9 and type II and X collagens in regenerated condyle resulting from the use of a functional appliance. Ninety, 3-week-old, mice were divided equally into the following groups: two experimental groups (condylectomy group and condylectomy with functional appliance group) and the corresponding control group. In the condylectomy group, a unilateral condylectomy was performed on the right side. In the condylectomy with appliance group, the mandible was repositioned in a forward direction using a functional appliance after unilateral condylectomy. The expression of Sox 9 and type II and X collagens in the condyle was determined immunohistochemically 4 weeks after surgery. In mice with a condylectomy, the expression was minimal. On the other hand, these factors were highly expressed in the condylectomized side with the appliance. It is thus speculated that cartilaginous regeneration is due to the expression of chondrogenic factors, such as Sox 9 and type II and X collagens. It is also suggested that condyle regeneration results from an optimal intra-articular environment with appropriate joint spaces achieved by condylar repositioning.
Fujita
Tadashi
T
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Minami-Ku, Hiroshima, Japan. seven@hiroshima-u.ac.jp
Nakano
Mao
M
Ohtani
Junji
J
Kawata
Toshitsugu
T
Kaku
Masato
M
Motokawa
Masahide
M
Tsuka
Natsumi
N
Hayashi
Hidetaka
H
Tanne
Kazuo
K
D000818:Animals; D020219:Chondrogenesis / Q000235:genetics*; D024043:Collagen Type II / Q000096:biosynthesis* / Q000235:genetics; D024121:Collagen Type X / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D019186:Mandibular Advancement / Q000295:instrumentation; D008335:Mandibular Condyle / Q000601:surgery*; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D017090:Occlusal Splints; D017289:Orthodontic Appliances, Functional; D010012:Osteogenesis / Q000235:genetics; D012038:Regeneration / Q000235:genetics*; D055758:SOX9 Transcription Factor / Q000096:biosynthesis / Q000235:genetics*
D024043:Collagen Type II; D024121:Collagen Type X; D055758:SOX9 Transcription Factor; C527933:Sox9 protein, mouse