Epidermal cellular response to poly(vinyl alcohol) nanofibers containing silver nanoparticles.
A heat-treated PVA nanofibrous matrix containing silver (Ag) was prepared by electrospinning an aqueous 10 wt% PVA solution and followed by heat treatment at 150 degrees C for 10 min. The average diameter of the as-spun and heat-treated PVA nanofibers was 330 nm. The heat-treated PVA nanofibrous matrix containing Ag was irradiated with UV light to transform the Ag ions in the nanofibrous matrix into Ag nanoparticles. The in vitro cytotoxicity of the Ag ions and/or nanoparticles on normal human epidermal keratinocytes (NHEK) and fibroblasts (NHEF) cultures was examined. The PVA nanofibrous matrix containing Ag showed slightly higher level of attachment and spreading in the early stage culture (1 h) than the PVA nanofibers without Ag (control). However, compared with the PVA nanofibers without Ag, the heat-treated and UV-irradiated PVA nanofibers, containing mainly Ag ions and nanoparticles, respectively, showed reduced cell attachment and spreading. This shows that both Ag ions and Ag nanoparticles are cytotoxic to NHEK and NHEF. There was no significant difference in cytotoxicity to NHEK and NHEF between Ag ions and Ag nanoparticles. NHEF appeared to be more sensitive to Ag ions or particles than NHEK. In addition, the residual nitrate ions (NO3(-)) in the PVA nanofibers had an adverse effect on the culture of both cells.
Chun
Ja Young
JY
Department of Advance Organic Materials and Textile System Engineering, Chungnam National University, Daejeon 305-764, South Korea.
Kang
Hyun Ki
HK
Jeong
Lim
L
Kang
Yun Ok
YO
Oh
Ju-Eun
JE
Yeo
In-Sung
IS
Jung
Sung Youn
SY
Park
Won Ho
WH
Min
Byung-Moo
BM
D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002675:Child, Preschool; D000078404:Epidermal Cells; D005347:Fibroblasts / Q000166:cytology* / Q000648:ultrastructure; D006358:Hot Temperature; D006801:Humans; D007223:Infant; D015603:Keratinocytes / Q000166:cytology* / Q000648:ultrastructure; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D057139:Nanofibers / Q000737:chemistry / Q000528:radiation effects; D011142:Polyvinyl Alcohol / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D014466:Ultraviolet Rays
D012834:Silver; D011142:Polyvinyl Alcohol