title: Increased numbers of myeloid and lymphoid IL-10 producing cells in spleen of pigs with naturally occurring postweaning multisystemic wasting syndrome.
abstract: Porcine circovirus type 2 (PCV2) is the essential etiological agent of postweaning multisystemic wasting syndrome (PMWS), a worldwide distributed pig disease. The involvement of the immune system in the pathogenesis of PMWS is considered crucial. Previous studies have shown a cytokine profile suggesting T immunosuppression and indicating that interleukin 10 (IL-10) may play an important role during PCV2 infection. Nine 11- to 12-week-old conventional pigs were obtained from commercial farms located in North-Eastern Spain with historical records of PMWS. Spleen from four healthy and five PMWS-affected animals were collected at the necropsy. Viral load was determined in serum by means of standard PCR and real-time quantitative PCR. Phenotype and distribution of different immune cells involved in IL-10 secretion in the spleen of studied pigs were analysed using immunofluorescent assays. The CD163(+), CD4(+), and CD8(+) cell subpopulations produced IL-10 in the spleen and IL-10(+) cell numbers were higher in PMWS animals compared with their healthy counterparts. Furthermore, IL-10 producing cells were not infected by PCV2 and were mainly localized in the periarteriolar lymphoid sheaths. This is the first immunophenotyping study on IL-10 producing cells in cases of PMWS, further extending the studies on the role of IL-10 in disease pathogenesis.
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Crisci
authors.forename: M
authors.initials: M
authors.lastname: Ballester
authors.forename: J
authors.initials: J
authors.lastname: Domínguez
authors.forename: J
authors.initials: J
authors.lastname: Segalés
authors.forename: M
authors.initials: M
authors.lastname: Montoya
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D016130:Immunophenotyping / Q000379:methods / Q000662:veterinary; D016753:Interleukin-10 / Q000276:immunology*; D008856:Microscopy, Fluorescence; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000276:immunology* / Q000821:virology; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000821:virology*; D013552:Swine; D019562:Viral Load / Q000276:immunology / Q000662:veterinary
chemical_list: D012367:RNA, Viral; D016753:Interleukin-10