HIV-1 Nef associates with p22-phox, a component of the NADPH oxidase protein complex.
Altered neutrophil function may contribute to the development of AIDS during the course of HIV infection. It has been described that Nef, a regulatory protein from HIV, can modulate superoxide production in other cells, therefore altered superoxide production in neutrophils from HIV infected patients, could be secondary to a direct effect of Nef on components of the NADPH oxidase complex. In this work, we describe that Nef, was capable of increasing superoxide production in human neutrophils. Furthermore, a specific association between Nef and p22-phox, a membrane component of the NADPH oxidase complex, was found. We propose that this association may reflect a capability of Nef to modulate by direct association, the enzymatic complex responsible for one of the most efficient innate defense mechanisms in phagocytes, contributing to the pathogenesis of the disease.
Salmen
Siham
S
Institute of Clinical Immunology, University of Los Andes, Merida, Venezuela.
Colmenares
Melisa
M
Peterson
Darrel L
DL
Reyes
Elbert
E
Rosales
Jose D
JD
Berrueta
Lisbeth
L
D003001:Cloning, Molecular; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015497:HIV-1 / Q000378:metabolism* / Q000472:pathogenicity; D006801:Humans; D009097:Multienzyme Complexes; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D000074624:NADPH Oxidase 1; D019255:NADPH Oxidases / Q000378:metabolism*; D054311:nef Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
D009097:Multienzyme Complexes; D054311:nef Gene Products, Human Immunodeficiency Virus; D009247:NADH, NADPH Oxidoreductases; D000074624:NADPH Oxidase 1; D019255:NADPH Oxidases; C086986:CYBA protein, human