title: Comparative transcriptome and secretome analysis of wood decay fungi Postia placenta and Phanerochaete chrysosporium.
abstract: Cellulose degradation by brown rot fungi, such as Postia placenta, is poorly understood relative to the phylogenetically related white rot basidiomycete, Phanerochaete chrysosporium. To elucidate the number, structure, and regulation of genes involved in lignocellulosic cell wall attack, secretome and transcriptome analyses were performed on both wood decay fungi cultured for 5 days in media containing ball-milled aspen or glucose as the sole carbon source. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), a total of 67 and 79 proteins were identified in the extracellular fluids of P. placenta and P. chrysosporium cultures, respectively. Viewed together with transcript profiles, P. chrysosporium employs an array of extracellular glycosyl hydrolases to simultaneously attack cellulose and hemicelluloses. In contrast, under these same conditions, P. placenta secretes an array of hemicellulases but few potential cellulases. The two species display distinct expression patterns for oxidoreductase-encoding genes. In P. placenta, these patterns are consistent with an extracellular Fenton system and include the upregulation of genes involved in iron acquisition, in the synthesis of low-molecular-weight quinones, and possibly in redox cycling reactions.
authors.affiliation: Forest Products Lab, One Gifford Pinchot Drive, Madison, WI 53726, USA.
authors.forename: Amber
authors.initials: A
authors.lastname: Vanden Wymelenberg
authors.forename: Jill
authors.initials: J
authors.lastname: Gaskell
authors.forename: Michael
authors.initials: M
authors.lastname: Mozuch
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Sabat
authors.forename: John
authors.initials: J
authors.lastname: Ralph
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Skyba
authors.forename: Shawn D
authors.initials: SD
authors.lastname: Mansfield
authors.forename: Robert A
authors.initials: RA
authors.lastname: Blanchette
authors.forename: Diego
authors.initials: D
authors.lastname: Martinez
authors.forename: Igor
authors.initials: I
authors.lastname: Grigoriev
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Kersten
authors.forename: Dan
authors.initials: D
authors.lastname: Cullen
mesh_terms: D002482:Cellulose / Q000378:metabolism; D002853:Chromatography, Liquid; D055453:Coriolaceae / Q000737:chemistry / Q000235:genetics*; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism*; D020869:Gene Expression Profiling; D016695:Glycosyltransferases / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D020075:Phanerochaete / Q000737:chemistry / Q000235:genetics*; D011134:Polysaccharides / Q000378:metabolism; D020543:Proteome; D053719:Tandem Mass Spectrometry; D014934:Wood / Q000378:metabolism / Q000382:microbiology*
chemical_list: D005656:Fungal Proteins; D011134:Polysaccharides; D020543:Proteome; C007916:hemicellulose; D002482:Cellulose; D010088:Oxidoreductases; D016695:Glycosyltransferases
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