Modulation of platelet aggregation, haematological and histological parameters by structured lipids on hypercholesterolaemic rats.
The effect of the consumption of medium-chain fatty acid (MCFA)-rich and polyunsaturated fatty acid (PUFA)-rich mustard oil on platelet aggregation, haematological parameters and the liver was studied in male albino rats. The rats were fed on standard stock diet with control (mustard oil) and experimental oils for 28 days. Haematological examinations in the normal condition showed that there was no significant variation in the platelet count, total white blood cell (WBC) and red blood cell (RBC) counts, haematocrit value and mean cell haemoglobin concentration (MCHC) percentage in the rats fed with control and experimental oils. Haematological examinations in the hypercholesterolaemic condition revealed that there was a significant increase in the platelet count by 39.38% in hypercholesterolaemia, which was decreased by 27.29 and 42.71% by the administration of the experimental oils, respectively. The haemoglobin level was decreased by 5.3%, whereas the haematocrit value was increased by 12.52% in hypercholesterolaemia, which were normalised by treatment with the experimental oils. The platelet aggregation study indicated that the adenosine diphosphate (ADP)-induced platelet aggregation increased by 71.67% in hypercholesterolaemia, but the experimental oils beneficially reduced platelet aggregation by 26.33 and 68.33%, respectively. There was increased total cholesterol, non-high-density lipoprotein (non-HDL) cholesterol and triglyceride levels in liver in hypercholesterolaemia, which was also recovered by the administration of experimental oils. Organopathological examination showed that there was deposition of cholesterol in the liver in the hypercholesterolaemic condition, which was also reduced by treatment with the two experimental oils.
Sengupta
Avery
A
Department of Chemical Technology, University College of Science and Technology, University of Calcutta, 92, APC Road, Kolkata 700009, India.
Ghosh
Mahua
M
D000818:Animals; D004195:Disease Models, Animal; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D006403:Hematologic Tests; D006937:Hypercholesterolemia; D050356:Lipid Metabolism; D008099:Liver / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D009149:Mustard Plant; D010938:Plant Oils / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D051381:Rats; D012015:Reference Standards
D005231:Fatty Acids, Unsaturated; D010938:Plant Oils; C027793:mustard oil
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