On-chip differential interference contrast microscopy using lensless digital holography.
We introduce the use of a birefringent crystal with lensless digital holography to create an on-chip differential interference contrast (DIC) microscope. Using an incoherent source with a large aperture, in-line holograms of micro-objects are created, which interact with a uniaxial crystal and an absorbing polarizer, encoding differential interference contrast information of the objects on the chip. Despite the fact that a unit fringe magnification and an incoherent source with a large aperture have been used, holographic digital processing of such holograms rapidly recovers the differential phase contrast image of the specimen over a large field-of-view of approximately 24 mm(2).
Oh
Chulwoo
C
Electrical Engineering Department, University of California, Los Angeles, CA, USA.
Isikman
Serhan O
SO
Khademhosseinieh
Bahar
B
Ozcan
Aydogan
A
J Opt Soc Am. 1967 Apr;57(4):546-7
6027838
Opt Express. 2006 Sep 4;14(18):8263-8
19529201
Opt Express. 2005 Oct 31;13(22):8693-8
19498901
Opt Lett. 2006 May 15;31(10):1405-7
16642120
Appl Opt. 2007 Apr 1;46(10):1836-42
17356628
J Microsc. 2002 Jun;206(Pt 3):194-203
12067363
J Opt Soc Am A Opt Image Sci Vis. 2006 Apr;23(4):926-32
16604777
Methods Cell Biol. 2008;90:87-115
19195547
Phys Rev Lett. 2000 Oct 16;85(16):3385-7
11030902
Opt Lett. 2004 May 15;29(10):1132-4
15182009
Opt Lett. 2007 Dec 15;32(24):3492-4
18087519
Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11301-5
11572982
Opt Lett. 1985 Jan 1;10(1):4-6
19724327
Opt Lett. 2006 May 1;31(9):1211-3
16642062
Opt Express. 2006 May 1;14(9):3792-805
19516527
Appl Opt. 1992 Aug 20;31(24):4973-8
20733659
Appl Opt. 2002 Aug 1;41(22):4489-96
12153076
Opt Lett. 1978 Jul 1;3(1):27-9
19684685