Peripheral blood dendritic cells and vascular endothelial growth factor in oral squamous cell carcinoma: correlation analysis and in vitro study.
Vascular endothelial growth factor (VEGF) may cause functional deficiency in dendritic cells (DCs) in vitro. The roles of peripheral blood dendritic cells (PBDCs) and VEGF in patients with oral squamous cell carcinoma (OSCC) are not well understood. The authors analysed the correlation between VEGF and PBDC in 81 OSCC patients. They assessed the effect of VEGF on DC function in vitro. VEGF levels were significantly increased in OSCC patients compared with control subjects (P < 0.05), but PBDC levels were significantly lower (P < 0.05). VEGF expression in TNM I-II (67%) and T1-T2 (74%) was significantly lower, compared with TNM III-IV (88%, P < 0.05) and T3-T4 (89%, P < 0.05). Increased VEGF expression in primary tumours was significantly correlated with elevated serum VEGF levels, but reduced PBDC levels. In vitro cultured DC exposed to VEGF showed significantly decreased expression of functional proteins, enhanced endocytosis activity, and elicited weaker proliferation of T cells, compared with that of free VEGF (P < 0.01). These findings suggest that decreased PBDC and elevated VEGF occur in OSCC patients. Higher VEGF levels may affect precursor cells, resulting in decreased numbers of functional DC.
Wang
Z-Y
ZY
Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Affiliated to Medical School, Nanjing University, Nanjing 210008, PR China.
Shi
P-H
PH
Huang
X-F
XF
Hua
Z-C
ZC
Hou
Y-Y
YY
Han
W
W
Hu
Q-G
QG
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000473:pathology*; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology*; D004705:Endocytosis / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000097:blood / Q000473:pathology*; D009367:Neoplasm Staging; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000097:blood* / Q000276:immunology
C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A