Ecotoxicology goes MudPIT?
Exposure to natural and anthropogenic compounds can potentially alter the proteome in body fluids and tissues of living organisms, and by applying proteomics it is possible to discover, identify and understand such alterations. This study show results from a proteomic approach where one- or multidimensional separation (MudPIT) combined with high-accuracy tandem mass spectrometry (i.e. LTQ Orbitrap) were used to identify proteins from a non-model organism (Salmo salar). An optimized two-dimensional method resulted in more than 680 proteins identified with high significance compared to 197 proteins identified using a one-dimensional separation. Thus, MudPIT proteomics greatly increase the number of successful protein identification studies in ecotoxicology, and could potentially provide more insight into chemical modes of actions.
Uleberg
Kai-Erik
KE
Pathology Department, Stavanger University Hospital, Armauer Hansens Road 20, Stavanger, Norway. keu@iris.no
Larssen
Eivind
E
Oysæd
Kjell Birger
KB
Mæland
Mari
M
Bjørnstad-Hjelle
Anne
A
D000818:Animals; D054750:Ecotoxicology / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D020317:Salmo salar / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D011506:Proteins