Proteomic analysis of the effects of ABA treatments on ripening Vitis vinifera berries.
The control of ripening of the non-climacteric grapevine fruit is still a matter of debate, but several lines of evidence point to an important role for the hormone abscisic acid (ABA). The effects of ABA treatments on Cabernet Sauvignon berries before and at véraison were studied using a 2-DE proteomic approach. Proteins from whole deseeded berries (before véraison) and berry flesh and skin (at véraison) treated with 0.76 mM ABA and collected 24 h after treatment were separated and analysed. A total of 60 protein spots showed significant variations between treated and control berries, and 40 proteins, mainly related to general metabolism and cell defence, were identified by LC MS/MS. Our results show that ABA acts mainly through the regulation of mostly the same proteins which are involved in the ripening process, and that several of these changes share common elements with the ABA-induced responses in vegetative tissues.
Giribaldi
Marzia
M
Università di Torino, Dipartimento Colture arboree, via Leonardo da Vinci 44, 10095 Grugliasco (TO), Italy. marzia.giribaldi@ispa.cnr.it
Gény
Laurence
L
Delrot
Serge
S
Schubert
Andrea
A
D000040:Abscisic Acid / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005638:Fruit / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D027843:Vitis / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*
D010940:Plant Proteins; D000040:Abscisic Acid
Nature. 1970 Aug 15;227(5259):680-5
5432063
Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9909-14
15197268
Plant Physiol. 2006 Jan;140(1):150-8
16361521
Plant J. 2004 May;38(3):381-95
15086800
Funct Integr Genomics. 2002 Nov;2(6):282-91
12444421
Plant Physiol. 1999 Jul;120(3):923
10398729
Funct Integr Genomics. 2005 Jan;5(1):40-58
15480888
BMC Genomics. 2008 Aug 08;9:378
18691399
Plant Cell. 2003 Sep;15(9):2165-80
12953118
Plant Physiol. 2003 Jul;132(3):1664-77
12857845
J Exp Bot. 2003 Feb;54(383):727-38
12554716
Plant J. 2007 May;50(3):414-28
17376162
J Cell Sci. 2002 Dec 15;115(Pt 24):4891-900
12432076
Nature. 2007 Sep 27;449(7161):463-7
17721507
New Phytol. 2000 Dec;148(3):357-396
33863019
J Exp Bot. 2007;58(7):1851-62
17426054
J Photochem Photobiol B. 1999 Feb;48(2-3):200-9
10343405
Trends Plant Sci. 2007 Aug;12(8):343-51
17629540
Plant Physiol. 1996 Aug;111(4):1059-1066
12226348
Plant Physiol. 2005 Oct;139(2):652-63
16169968
Plant Mol Biol. 2004 Mar;54(5):713-25
15356390
Planta. 2005 Nov;222(5):832-47
16151847
J Exp Bot. 2009;60(6):1579-88
19246595
Plant Physiol. 2005 Nov;139(3):1350-65
16258012
J Exp Bot. 2000 Apr;51(345):675-83
10938859
Plant Physiol. 1976 Oct;58(4):468-72
16659699
Proteomics. 2007 Sep;7(17):3154-70
17683049
Plant Cell. 2002;14 Suppl:S15-45
12045268
Electrophoresis. 2004 May;25(9):1327-33
15174055
Plant Physiol. 2006 Sep;142(1):220-32
16861573
BMC Genomics. 2007 Nov 22;8:429
18034876
Plant Physiol. 1997 Nov;115(3):1155-1161
12223864
Eur J Biochem. 2004 Oct;271(20):4123-31
15479241
BMC Genomics. 2009 May 08;10:212
19426499
Plant Sci. 2000 Aug 8;157(1):77-88
10940471
Plant Physiol. 1997 Jul;114(3):771-8
9232868
J Agric Food Chem. 1999 Dec;47(12):4837-41
10606539
Plant Cell. 2004 Dec;16(12):3413-25
15548746
Plant Physiol. 2000 Mar;122(3):803-12
10712544
Plant Cell. 2004 Dec;16(12):3460-79
15548743
Plant J. 2008 Mar;53(5):717-30
17988221
J Exp Bot. 2008;59(11):2979-90
18550598
BMC Genomics. 2007 Nov 22;8:428
18034875
Planta. 2001 Dec;214(2):257-64
11800390
Plant Physiol. 2005 Oct;139(2):574-97
16219919
Plant J. 2004 Jan;37(1):104-14
14675436
Biochem J. 2003 Jul 1;373(Pt 1):289-96
12678916
Plant Physiol Biochem. 2009 Apr;47(4):282-90
19136268
Plant Physiol. 1973 Apr;51(4):629-34
16658384
Plant Cell. 1999 Oct;11(10):1897-910
10521520
Plant Physiol. 1996 May;111(1):275-83
8685267