title: High throughput and wide field of view EUV microscope for blur-free one-shot imaging of living organisms.
abstract: We present and demonstrate the use of an extreme ultraviolet (EUV) microscope that was developed in-house. Images are acquired using Bragg reflection multilayer optics and a laser-produced plasma light source. The upper-limit spatial resolution of the EUV microscope is 130 nm with a 10 ns exposure time and 250 x 250 microm(2) field of view. Resolution is superior to that of visible microscopes with the same size of field of view, and the exposure time is short enough to observe fine structures in-vivo. Observation of the cerebral cortex of a mouse is demonstrated.
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1, Katahira, Aoba-ku, Sendai, 980-8577, Japan. ejima@tagen.tohoku.ac.jp
authors.forename: Takeo
authors.initials: T
authors.lastname: Ejima
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Ishida
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Murata
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Toyoda
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Harada
authors.forename: Toshihide
authors.initials: T
authors.lastname: Tsuru
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hatano
authors.forename: Mihiro
authors.initials: M
authors.lastname: Yanagihara
authors.forename: Masaki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Haruo
authors.initials: H
authors.lastname: Mizutani
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000473:pathology; D004577:Electron Probe Microanalysis / Q000379:methods*; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008853:Microscopy / Q000379:methods*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D055095:Optics and Photonics; D011108:Polymers / Q000737:chemistry; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D011108:Polymers