title: LC-MS/MS analysis of carboxymethylated and carboxyethylated phosphatidylethanolamines in human erythrocytes and blood plasma.
abstract: An amino group of phosphatidylethanolamine (PE) is considered as a target for nonenzymatic glycation, and the potential involvement of lipid glycation in the pathogenesis of diabetic complications has generated interest. However, unlike an early glycation product of PE (Amadori-PE), the occurrence and roles of advanced glycation end products of PE (AGE-PE) in vivo have been unclear. Here, we developed an LC-MS/MS method for the analysis of AGE-PE [carboxymethyl-PE (CM-PE) and carboxyethyl-PE (CE-PE)]. Collision-induced dissociation of CM-PE and CE-PE produced characteristic ions, permitting neutral loss scanning (NLS) and multiple reaction monitoring (MRM) of AGE-PE. By NLS analysis, a series of AGE-PE molecular species was detected in human erythrocytes and blood plasma. In LC-MS/MS analysis, MRM enabled the separation and determination of the predominant AGE-PE species. Between healthy subjects and diabetic patients, no significant differences were observed in AGE-PE concentrations in erythrocytes and plasma, whereas Amadori-PE concentrations were higher in diabetic patients. These results provide direct evidence for the presence of AGE-PE in human blood, and indicated that, compared with Amadori-PE, AGE-PE is less likely to be accumulated in diabetic blood. The presently developed LC-MS/MS method appears to be a powerful tool for understanding in vivo lipid glycation and its pathophysiological consequence.
authors.affiliation: Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Shoji
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Akira
authors.initials: A
authors.lastname: Asai
authors.forename: Ikuko
authors.initials: I
authors.lastname: Fujita
authors.forename: Aya
authors.initials: A
authors.lastname: Hashiura
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Shinichi
authors.initials: S
authors.lastname: Oikawa
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazawa
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods*; D003920:Diabetes Mellitus / Q000097:blood / Q000378:metabolism; D004912:Erythrocytes / Q000737:chemistry*; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood / Q000378:metabolism; D006801:Humans; D006943:Hyperglycemia / Q000097:blood / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010714:Phosphatidylethanolamines / Q000097:blood* / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D055815:Young Adult
chemical_list: D017127:Glycation End Products, Advanced; D010714:Phosphatidylethanolamines; C483858:phosphatidylethanolamine
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