title: A colorimetric method for detection of K-ras codon 12 point mutations in DNA extracted from tissue and peripheral blood in pancreatic disorders.
abstract: Molecular-based methods to monitor point mutations require special and expensive equipment unavailable in most hospitals. Colorimetric-based analysis is an ideal platform for K-ras codon 12 gene point mutations because it uses commonly found hospital equipment. The colorimetric assay is sensitive and specific, detecting mutated DNA levels as low as 1% in a wild-type background. Paired genomic DNA extracts of fixed tissue and cellular fractions of peripheral blood are more sensitive and accurate than unpaired samplings. This approach has the potential to improve K-ras point mutation scans as well as to detect micrometastases in circulating tumor cells.
authors.affiliation: Department of Surgery, St. Vincent's Hospital Manhattan, New York, NY 10011, USA. rao48@yahoo.com
authors.forename: Robert A
authors.initials: RA
authors.lastname: Ollar
authors.forename: Avram M
authors.initials: AM
authors.lastname: Cooperman
authors.forename: Michael E
authors.initials: ME
authors.lastname: Wayne
authors.forename: James F
authors.initials: JF
authors.lastname: Barrecchia
authors.forename: Niket
authors.initials: N
authors.lastname: Sonpal
authors.forename: Sushil
authors.initials: S
authors.lastname: Duddempudi
authors.forename: Franklin E
authors.initials: FE
authors.lastname: Kasmin
mesh_terms: D003062:Codon / Q000235:genetics*; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000097:blood* / Q000235:genetics*; D006801:Humans; D010182:Pancreatic Diseases / Q000097:blood / Q000235:genetics* / Q000473:pathology; D017354:Point Mutation / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins / Q000235:genetics*
chemical_list: D003062:Codon; C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; D004247:DNA; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins