title: Skin absorption studies of octyl-methoxycinnamate loaded poly(D,L-lactide) nanoparticles: estimation of the UV filter distribution and release behaviour in skin layers.
abstract: New formulation strategies have to be developed to limit the skin penetration of UV-filter. Nanoparticles (NP) are very suitable for that purpose. In this study, the skin distribution, at different times (1, 2 and 3 h), of octyl-methoxycinnamate (OMC) from loaded PLA-nanoparticles was compared to a classical formulation containing non-encapsulated OMC, using the Franz cell method. The results showed that the OMC penetration was clearly impeded by stratum corneum and that the major part of the OMC-NP was accumulated at the skin surface (> 80%). A significant lower OMC amount was quantified in viable skin with NP compared to the OMC emulgel. To accurately determine the real OMC amount in close contact with viable skin layers two solvents were used to extract OMC from the skin compartments. Acetone (ACET) allowed quantifying both OMC in NP and OMC released from the particles, while isopropylmyristate (IPM), a non-solvent of the NP polymer (PLA), allowed quantifying only OMC released from the particles. Using IPM as an extraction solvent, it appeared that the OMC released from NP, in contact with viable skin, was 3-fold lower than free OMC diffused from the emulgel. Lastly, a sustained release was observed when nanoparticles were used.
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Pavia, Pavia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Vettor
authors.forename: S
authors.initials: S
authors.lastname: Bourgeois
authors.forename: H
authors.initials: H
authors.lastname: Fessi
authors.forename: J
authors.initials: J
authors.lastname: Pelletier
authors.forename: P
authors.initials: P
authors.lastname: Perugini
authors.forename: F
authors.initials: F
authors.lastname: Pavanetto
authors.forename: M A
authors.initials: MA
authors.lastname: Bolzinger
mesh_terms: D000818:Animals; D002934:Cinnamates / Q000008:administration & dosage* / Q000493:pharmacokinetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry*; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D013473:Sunscreening Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D013552:Swine
chemical_list: D002934:Cinnamates; D011091:Polyesters; D013473:Sunscreening Agents; C033616:poly(lactide); C118580:octylmethoxycinnamate