title: Induction of apoptosis in densovirus infected Aedes aegypti mosquitoes.
abstract: The mechanism of death in densovirus infected mosquitoes remains unexplored. This study investigated the cellular consequences of densovirus infection in Aedes aegypti mosquitoes after a second generation challenge with a densovirus isolated from adult Aedes albopictus mosquitoes in Thailand (AThDNV). Specimens were analyzed by TUNEL assay, fluorescent in situ hybridization (FISH) and a calorimic assay to detect activation of caspase 3-like activity. After challenge, moribund mosquitoes showed considerable evidence of TUNEL positive cells. The caspase 3-like activity assay showed that the presence of TUNEL positive cells was associated with increased levels of activated caspase 3-like activity in AThDNV infected mosquitoes.
authors.affiliation: Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, Thailand.
authors.forename: Songsak
authors.initials: S
authors.lastname: Roekring
authors.forename: Duncan R
authors.initials: DR
authors.lastname: Smith
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3; D004266:DNA Virus Infections / Q000473:pathology / Q000635:transmission / Q000662:veterinary*; D004279:DNA, Viral / Q000032:analysis; D019223:Densovirinae / Q000235:genetics / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D017404:In Situ Hybridization, Fluorescence; D020287:In Situ Nick-End Labeling; D019476:Insect Proteins; D007303:Insect Vectors / Q000821:virology*; D007814:Larva / Q000821:virology; D009032:Mosquito Control; D010572:Pest Control, Biological
chemical_list: D004279:DNA, Viral; D019476:Insect Proteins; D053148:Caspase 3