title: Detection of white spot syndrome virus (WSSV) in the Pacific oyster Crassostrea gigas.
abstract: Oysters Crassostrea gigas were placed at water supply canals of three shrimp farms in Guasave, Mexico where WSSV outbreaks occur. Animals were sampled through April-August and September-December to detect WSSV DNA. By using three different PCR protocols, only oysters from a farm undergoing a WSSV outbreak were found WSSV-positive in gills and digestive gland. Two WSSV amplicons were sequenced and they corresponded over 99% to WSSV genome segments. Results showed that oysters can capture WSSV particles suspended in water. Susceptibility of oysters to WSSV infection and their role as a carrier remain to be determined.
authors.affiliation: Centro de Investigaciones Biológicas del Noroeste (CIBNOR), La Paz, BCS, Mexico.
authors.forename: Celia
authors.initials: C
authors.lastname: Vazquez-Boucard
authors.forename: Pindaro
authors.initials: P
authors.lastname: Alvarez-Ruiz
authors.forename: Cristina
authors.initials: C
authors.lastname: Escobedo-Fregoso
authors.forename: Gerardo
authors.initials: G
authors.lastname: Anguiano-Vega
authors.forename: Ma de Jesus
authors.initials: Mde J
authors.lastname: Duran-Avelar
authors.forename: Vania Serrano
authors.initials: VS
authors.lastname: Pinto
authors.forename: César Marcial
authors.initials: CM
authors.lastname: Escobedo-Bonilla
mesh_terms: D000818:Animals; D002353:Carrier State / Q000662:veterinary / Q000821:virology; D049896:Crassostrea / Q000821:virology*; D004266:DNA Virus Infections / Q000175:diagnosis / Q000635:transmission / Q000662:veterinary*; D004279:DNA, Viral / Q000235:genetics; D004064:Digestive System / Q000821:virology; D005880:Gills / Q000821:virology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012758:Shellfish / Q000821:virology*; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis; D046848:White spot syndrome virus 1 / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral