Differential cell surface expression of rhesus macaque's major histocompatibility complex class I alleles Mamu-B*1703 and Mamu-B*0101.
The major histocompatibility complex class I allele Mamu-B*17 of rhesus macaques is an elite controller of simian immunodeficiency virus (SIV) infection whereas Mamu-B*01 has no inhibitory effect on SIV replication. The mechanism is still elusive. In this study, the so-called "missing G" in the leading peptide sequence of Mamu-B*1703 allele was artificially inserted back through PCR amplification, and the new sequence was renamed as Mamu-B*1703(+G). The plasmids harboring Mamu-B*1703, Mamu-B*1703(+G) and Mamu-B*0101 cDNA sequence fused to EGFP gene were transfected into K562 and Cos-7 cells, respectively. Our data showed that these plasmids had similar transfection efficiencies and expression potentials in K562 cells, but the surface density of Mamu-B*1703 complexes, which was slightly influenced by the artificial change of the leading peptide length, was much higher than that of Mamu-B*0101 molecules. These results might partially account for the differential effects of Mamu-B*17 and Mamu-B*01 alleles on SIV replication in rhesus macaques.
Ouyang
Dongyun
D
Jinan University, Guangzhou, China.
He
Xianhui
X
Xu
Lihui
L
Wang
Xichao
X
Gao
Qi
Q
Guo
He
H
D000483:Alleles; D000818:Animals; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism*; D002522:Chlorocebus aethiops; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D008285:Major Histocompatibility Complex; D010455:Peptides / Q000737:chemistry; D015302:Simian Immunodeficiency Virus / Q000235:genetics*
D015395:Histocompatibility Antigens Class I; C092249:Mamu-B 01 antigen, Macaca mulatta; D010455:Peptides; D049452:Green Fluorescent Proteins