Image-based calibration of a deformable mirror in wide-field microscopy.
Optical aberrations limit resolution in biological tissues, and their influence is particularly large for promising techniques such as light-sheet microscopy. In principle, image quality might be improved by adaptive optics (AO), in which aberrations are corrected by using a deformable mirror (DM). To implement AO in microscopy, one requires a method to measure wavefront aberrations, but the most commonly used methods have limitations for samples lacking point-source emitters. Here we implement an image-based wavefront-sensing technique, a variant of generalized phase-diverse imaging called multiframe blind deconvolution, and exploit it to calibrate a DM in a light-sheet microscope. We describe two methods of parameterizing the influence of the DM on aberrations: a traditional Zernike expansion requiring 1040 parameters, and a direct physical model of the DM requiring just 8 or 110 parameters. By randomizing voltages on all actuators, we show that the Zernike expansion successfully predicts wavefronts to an accuracy of approximately 30 nm (rms) even for large aberrations. We thus show that image-based wavefront sensing, which requires no additional optical equipment, allows a simple but powerful method to calibrate a deformable optical element in a microscope setting.
Turaga
Diwakar
D
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Holy
Timothy E
TE
D000465:Algorithms; D000818:Animals; D002138:Calibration; D003116:Color; D004867:Equipment Design; D006801:Humans; D008853:Microscopy / Q000379:methods*; D015233:Models, Statistical; D016011:Normal Distribution; D055095:Optics and Photonics; D012031:Refractometry; D015203:Reproducibility of Results; D012107:Research Design; D014867:Water / Q000737:chemistry
D014867:Water
Philos Trans A Math Phys Eng Sci. 2007 Dec 15;365(1861):2829-43
17855218
Opt Express. 2002 Jan 28;10(2):145-54
19424342
Opt Lett. 2009 Aug 15;34(16):2495-7
19684827
Opt Lett. 2008 Oct 15;33(20):2302-4
18923603
Appl Opt. 2000 Jan 1;39(1):94-103
18337874
Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5788-92
11959908
Neuron. 2008 Mar 13;57(5):661-72
18341987
Opt Express. 2006 Oct 2;14(20):8900-17
19529270
Nat Methods. 2007 Apr;4(4):331-6
17384643
J Biomed Opt. 2005 Jul-Aug;10(4):44014
16178647
Opt Lett. 2004 Oct 1;29(19):2255-7
15524372
Science. 2004 Aug 13;305(5686):1007-9
15310904
J Microsc. 2004 Oct;216(Pt 1):32-48
15369481
Appl Opt. 2003 Oct 10;42(29):5807-15
14577533
Opt Lett. 2004 Sep 15;29(18):2142-4
15460883