title: Validated LC-MS/MS method for the determination of sarpogrelate in human plasma: application to a pharmacokinetic and bioequivalence study in Chinese volunteers.
abstract: A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for the determination of sarpogrelate in human plasma. One-step protein precipitation with acetonitrile was used to extract the analytes from the plasma. Sarpogrelate and tramadol (internal standard, I.S.) were separated on a Venusil MP-C(18) column within 1.7 min, using acetonitrile:ammonium acetate (10 mM, pH 6.8) (55:45, v/v) as mobile phase at a flow rate of 1.2 mL/min with an approximately 1:1 split entering the mass spectrometer. Detection was performed on electrospray positive ionization mass spectrometry by multiple reaction monitoring of the transitions of sarpogrelate at m/z 430.3-->135.3 and of I.S. at m/z 264.1-->58.0. The assay was validated over the concentration range of 1-1000 ng/mL with a lower limit of quantitation (LLOQ) of 1 ng/mL using 50 microL of plasma. The intra- and inter-day precision (relative standard deviation, R.S.D.) were <or=6.4% and <or=5.4%, respectively, with accuracy (relative error, R.E.) in the range 0.5-3.6%. The method was successfully applied to a pharmacokinetic and bioequivalence study enrolling 22 Chinese volunteers administered sarpogrelate tablets.
authors.affiliation: Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130021, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Yantong
authors.initials: Y
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Guoqing
authors.initials: G
authors.lastname: Li
authors.forename: Jingkai
authors.initials: J
authors.lastname: Gu
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods*; D018592:Cross-Over Studies; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012702:Serotonin Antagonists / Q000097:blood*; D013386:Succinates / Q000097:blood* / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013810:Therapeutic Equivalency
chemical_list: D012702:Serotonin Antagonists; D013386:Succinates; C064294:sarpogrelate