Detection of white spot syndrome virus (WSSV) in the Pacific oyster Crassostrea gigas.
Oysters Crassostrea gigas were placed at water supply canals of three shrimp farms in Guasave, Mexico where WSSV outbreaks occur. Animals were sampled through April-August and September-December to detect WSSV DNA. By using three different PCR protocols, only oysters from a farm undergoing a WSSV outbreak were found WSSV-positive in gills and digestive gland. Two WSSV amplicons were sequenced and they corresponded over 99% to WSSV genome segments. Results showed that oysters can capture WSSV particles suspended in water. Susceptibility of oysters to WSSV infection and their role as a carrier remain to be determined.
Vazquez-Boucard
Celia
C
Centro de Investigaciones Biológicas del Noroeste (CIBNOR), La Paz, BCS, Mexico.
Alvarez-Ruiz
Pindaro
P
Escobedo-Fregoso
Cristina
C
Anguiano-Vega
Gerardo
G
Duran-Avelar
Ma de Jesus
Mde J
Pinto
Vania Serrano
VS
Escobedo-Bonilla
César Marcial
CM
D000818:Animals; D002353:Carrier State / Q000662:veterinary / Q000821:virology; D049896:Crassostrea / Q000821:virology*; D004266:DNA Virus Infections / Q000175:diagnosis / Q000635:transmission / Q000662:veterinary*; D004279:DNA, Viral / Q000235:genetics; D004064:Digestive System / Q000821:virology; D005880:Gills / Q000821:virology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012758:Shellfish / Q000821:virology*; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis; D046848:White spot syndrome virus 1 / Q000302:isolation & purification*
D004279:DNA, Viral