title: Upregulation of phagocytic clearance of apoptotic cells by autoimmune regulator.
abstract: To investigate the effect of autoimmune regulator (AIRE) on phagocytic clearance of apoptotic cells, a recombinant expression vector containing full-length human AIRE cDNA was transfected into 16HBE cells. After incubation with transfected 16HBE cells, engulfment of apoptotic HL-60 cells induced by camptothecin was detected by myeloperoxidase (MPO) staining. The change in the expression of Rac 1 in transfected 16HBE cells was determined by RT-PCR and Western blotting. The results showed that the phagocytosis percentage of the experimental group, the mock transfection group and the negative control group (non-apoptotic cells) was (25.50 + or - 3.67)%, (6.25 + or - 1.58)% and (1.0 + or - 0.67)%, respectively. Moreover, the expressions of Rac 1 mRNA and protein were up-regulated in AIRE-transfected 16HBE cells, suggesting that AIRE may function as a regulator in the phagocytic clearance of apoptotic cells by promoting the expression of Rac 1.
authors.affiliation: Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. liangshi_7@126.com
authors.forename: Liang
authors.initials: L
authors.lastname: Shi
authors.forename: Lihua
authors.initials: L
authors.lastname: Hu
authors.forename: Yirong
authors.initials: Y
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D015551:Autoimmunity / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005822:Genetic Vectors; D006098:Granulocytes / Q000166:cytology; D018922:HL-60 Cells; D006801:Humans; D010586:Phagocytes / Q000502:physiology; D010587:Phagocytosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*; D000096927:AIRE Protein
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D014157:Transcription Factors; D020830:rac1 GTP-Binding Protein; D000096927:AIRE Protein; C107953:RAC1 protein, human
references.citation: J Clin Endocrinol Metab. 1998 Apr;83(4):1049-55
references.pmid: 9543115
references.citation: Nat Genet. 1997 Dec;17(4):393-8
references.pmid: 9398839
references.citation: Dev Cell. 2001 Oct;1(4):491-502
references.pmid: 11703940
references.citation: J Exp Med. 2000 Feb 7;191(3):435-44
references.pmid: 10662789
references.citation: Nat Cell Biol. 2000 Mar;2(3):131-6
references.pmid: 10707082
references.citation: Science. 2002 Nov 15;298(5597):1395-401
references.pmid: 12376594
references.citation: Biochem Biophys Res Commun. 1999 Apr 21;257(3):821-5
references.pmid: 10208866
references.citation: Cell. 2001 Oct 5;107(1):27-41
references.pmid: 11595183
references.citation: Arthritis Rheum. 1998 Jul;41(7):1241-50
references.pmid: 9663482
references.citation: Nat Immunol. 2001 Nov;2(11):1010-7
references.pmid: 11590405
references.citation: Semin Cell Dev Biol. 2005 Apr;16(2):295-306
references.pmid: 15797839
references.citation: J Biol Chem. 2002 Apr 5;277(14):11772-9
references.pmid: 11729193
references.citation: Nature. 1998 Apr 2;392(6675):505-9
references.pmid: 9548256
references.citation: Cell. 1998 Jun 12;93(6):951-60
references.pmid: 9635425
references.citation: Immunol Lett. 2002 Mar 1;80(3):195-8
references.pmid: 11803052
references.citation: Cell Death Differ. 2001 Jun;8(6):564-8
references.pmid: 11536006
references.citation: Mol Cell. 2004 May 7;14(3):277-87
references.pmid: 15125832
references.citation: Cell Death Differ. 1999 Jan;6(1):6-12
references.pmid: 10200542
references.citation: Proc Natl Acad Sci U S A. 2005 May 17;102(20):7233-8
references.pmid: 15883360
references.citation: Biochim Biophys Acta. 2000 Feb 28;1495(3):237-49
references.pmid: 10699463
references.citation: Nat Genet. 1997 Dec;17(4):399-403
references.pmid: 9398840
references.citation: Immunity. 2005 Aug;23(2):227-39
references.pmid: 16111640
references.citation: J Allergy Clin Immunol. 2009 Dec;124(6):1161-78
references.pmid: 20004777
references.citation: J Biol Chem. 2004 Aug 6;279(32):33984-91
references.pmid: 15150263
references.citation: Clin Immunol. 2006 Dec;121(3):265-73
references.pmid: 16982213