title: [A method of detect the virus which caused diarrhea].
abstract: OBJECTIVE
  Building a method which can examines virus pathogenic in gastroenteritis excrement specimen.
  
  
  METHODS
  Choosing six positive specimens which tested in our laboratory, include adenovirus, calicivirus, rotavirus, bocavirus, astrovirus and enterovirus. Through sequence-independent single primer amplification(SISPA) constructs a gene bank. Looks up the viral gene fragment in gene bank.
  
  
  RESULTS
  Obtaining corresponding viral acid sequence in six specimens.
  
  
  CONCLUSIONS
  This research can examine enterovirus and the virus which cause diarrhea, It make a foundation for further studies the viral cause of disease which the examination not yet discovered at present.
authors.affiliation: College of Veterinary Medicine NWSUAF, Yangling 712100, China.
authors.forename: Da-jiang
authors.initials: DJ
authors.lastname: Cao
authors.forename: Xian-jun
authors.initials: XJ
authors.lastname: Zhao
authors.forename: Wei-xia
authors.initials: WX
authors.lastname: Cheng
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.forename: Dan-di
authors.initials: DD
authors.lastname: Li
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Shu-xian
authors.initials: SX
authors.lastname: Cui
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics; D003967:Diarrhea / Q000175:diagnosis / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis / Q000821:virology*; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers