title: The mitochondrial genome of the pathogenic yeast Candida subhashii: GC-rich linear DNA with a protein covalently attached to the 5' termini.
abstract: As a part of our initiative aimed at a large-scale comparative analysis of fungal mitochondrial genomes, we determined the complete DNA sequence of the mitochondrial genome of the yeast Candida subhashii and found that it exhibits a number of peculiar features. First, the mitochondrial genome is represented by linear dsDNA molecules of uniform length (29 795 bp), with an unusually high content of guanine and cytosine residues (52.7 %). Second, the coding sequences lack introns; thus, the genome has a relatively compact organization. Third, the termini of the linear molecules consist of long inverted repeats and seem to contain a protein covalently bound to terminal nucleotides at the 5' ends. This architecture resembles the telomeres in a number of linear viral and plasmid DNA genomes classified as invertrons, in which the terminal proteins serve as specific primers for the initiation of DNA synthesis. Finally, although the mitochondrial genome of C. subhashii contains essentially the same set of genes as other closely related pathogenic Candida species, we identified additional ORFs encoding two homologues of the family B protein-priming DNA polymerases and an unknown protein. The terminal structures and the genes for DNA polymerases are reminiscent of linear mitochondrial plasmids, indicating that this genome architecture might have emerged from fortuitous recombination between an ancestral, presumably circular, mitochondrial genome and an invertron-like element.
authors.affiliation: Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 842 15 Bratislava, Slovak Republic.
authors.forename: Dominika
authors.initials: D
authors.lastname: Fricova
authors.affiliation: Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 842 15 Bratislava, Slovak Republic.
authors.forename: Matus
authors.initials: M
authors.lastname: Valach
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Kozep fasor 52, H-6726 Szeged, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Farkas
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Kozep fasor 52, H-6726 Szeged, Hungary.
authors.forename: Ilona
authors.initials: I
authors.lastname: Pfeiffer
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Kozep fasor 52, H-6726 Szeged, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Kucsera
authors.affiliation: Department of Genetics, Comenius University, Faculty of Natural Sciences, Mlynska dolina B-1, 842 15 Bratislava, Slovak Republic.
authors.forename: Lubomir
authors.initials: L
authors.lastname: Tomaska
authors.affiliation: Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 842 15 Bratislava, Slovak Republic.
authors.forename: Jozef
authors.initials: J
authors.lastname: Nosek
mesh_terms: D000595:Amino Acid Sequence; D002175:Candida / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D002177:Candidiasis / Q000382:microbiology; D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020862:GC Rich Sequence; D054629:Genome, Mitochondrial; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D011485:Protein Binding; D016415:Sequence Alignment
chemical_list: D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins
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