title: Development and characterization of monoclonal antibodies against Rift Valley fever virus nucleocapsid protein generated by DNA immunization.
abstract: This paper describes the generation of monoclonal antibodies directed to immunogenic nucleoprotein N epitopes of Rift Valley fever virus (RVFV), and their application in diagnostics, both for antibody detection in competitive ELISA and for antigen capture in a sandwich ELISA. Monoclonal antibodies (mAbs) were generated after DNA immunization of Balb/c mice and characterized by western blot, ELISA and cell immunostaining assays. At least three different immunorelevant epitopes were defined by mAb competition assays. Interestingly, two of the mAbs generated were able to distinguish between RVFV strains from Egyptian or South African lineages. These monoclonal antibodies constitute useful tools for diagnosis, especially for the detection of serum anti-RVFV antibodies from a broad range of species by means of competitive ELISA.
authors.affiliation: Centro de Investigación en Sanidad Animal, Carretera de Valdeolmos, Valdeolmos, Madrid, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Martín-Folgar
authors.forename: Gema
authors.initials: G
authors.lastname: Lorenzo
authors.forename: Hani
authors.initials: H
authors.lastname: Boshra
authors.forename: Javier
authors.initials: J
authors.lastname: Iglesias
authors.forename: Francisco
authors.initials: F
authors.lastname: Mateos
authors.forename: Belén
authors.initials: B
authors.lastname: Borrego
authors.forename: Alejandro
authors.initials: A
authors.lastname: Brun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007114:Immunization; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000096:biosynthesis / Q000276:immunology*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D012295:Rift Valley Fever / Q000097:blood / Q000175:diagnosis*; D012296:Rift Valley fever virus / Q000276:immunology*; D016415:Sequence Alignment
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins
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