Characterization of dental caries by LIF spectroscopy with 404-nm excitation.
The potential of laser-induced fluorescence (LIF) spectroscopy for the characterization of different stages of dental caries using 404-nm diode laser excitation was investigated. In vitro spectra from 16 sound, 10 noncavitated carious and 10 cavitated carious molar teeth were recorded on a miniature fibre-optic spectrometer. The areas under the receiver operating characteristics (ROC-AUC) were calculated and one-way analysis of variance (ANOVA) was performed. The LIF spectra of the carious teeth showed two peaks at 635 and 680 nm in addition to a broad band seen at 500 nm in sound teeth. The fluorescence intensity ratios, F500/F635 and F500/F680, in carious teeth were always lower than those in sound teeth. The ROC-AUC for discriminating between carious and sound teeth was 0.94, and for discriminating between noncavitated and cavitated carious teeth was 0.87. Statistically significant differences (p<0.001) were seen between sound, noncavitated carious and cavitated carious teeth. The results showed that LIF spectroscopy has the potential to be useful for characterizing different stages of caries in a clinical setting.
Thomas
Shiny Sara
SS
Biophotonics Laboratory, Centre for Earth Science Studies, Trivandrum, India.
Jayanthi
J L
JL
Subhash
Narayanan
N
Thomas
Joji
J
Mallia
Rupananda J
RJ
Aparna
G  N
G
D003731:Dental Caries / Q000145:classification / Q000175:diagnosis*; D006801:Humans; D054023:Lasers, Semiconductor; D055100:Optical Fibers; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
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