Vibrio mangrovi sp. nov., a diazotrophic bacterium isolated from mangrove-associated wild rice (Poteresia coarctata Tateoka).
The taxonomic status of a nitrogen-fixing bacterium, strain MSSRF38(T), isolated from the rhizosphere of mangrove-associated wild rice (Porteresia coarctata Tateoka), in Pichavaram, India, was studied using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the novel strain MSSRF38(T) was most closely related to Vibrio ruber DSM 16370(T) (98.3% gene sequence similarity), Vibrio rhizosphaerae DSM 18581(T) (98.2% sequence similarity) and <96% to the remaining Vibrio species. Multilocus sequence analysis using ftsZ, gapA, gyrB and mreB genes showed low levels of gene sequence similarities (82-90%) with all species of the genus Vibrio with validly published names, indicating that strain MSSRF38(T) occupies a distinct phylogenetic position. DNA-DNA hybridization experiments showed that strain MSSRF38(T) had <70% DNA-DNA similarity to its closest neighbours V. ruber DSM 16370(T) (27.4%) and V. rhizosphaerae DSM 18581(T) (12.1%). Strain MSSRF38(T) could be differentiated from its relatives on the basis of several phenotypic characteristics. The major fatty acids were feature 3 (including C(16:1omega)7c and/or C(15:0) iso 2-OH), C16:0, C(18:1omega)7c, C(14:0) and C(12:0). The DNA G+C content was 45.4 mol%. Based on genotypic, phenotypic, chemotaxonomic and DNA-DNA analyses, the name Vibrio mangrovi sp. nov. (type strain MSSRF38(T)=LMG 24290(T)=DSM 19641(T)) is proposed for this novel taxon.
Rameshkumar
N
N
Microbiology Department, M.S. Swaminathan Research Foundation, Chennai, India. rammicro@gmail.com
Sproer
Cathrin
C
Lang
Elke
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Nair
Sudha
S
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S