[A method of detect the virus which caused diarrhea].
OBJECTIVE
Building a method which can examines virus pathogenic in gastroenteritis excrement specimen.


METHODS
Choosing six positive specimens which tested in our laboratory, include adenovirus, calicivirus, rotavirus, bocavirus, astrovirus and enterovirus. Through sequence-independent single primer amplification(SISPA) constructs a gene bank. Looks up the viral gene fragment in gene bank.


RESULTS
Obtaining corresponding viral acid sequence in six specimens.


CONCLUSIONS
This research can examine enterovirus and the virus which cause diarrhea, It make a foundation for further studies the viral cause of disease which the examination not yet discovered at present.
Cao
Da-jiang
DJ
College of Veterinary Medicine NWSUAF, Yangling 712100, China.
Zhao
Xian-jun
XJ
Cheng
Wei-xia
WX
Jin
Miao
M
Li
Dan-di
DD
Zhang
Qin
Q
Cui
Shu-xian
SX
Duan
Zhao-jun
ZJ
D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics; D003967:Diarrhea / Q000175:diagnosis / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis / Q000821:virology*; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers