title: Delivery of paclitaxel from cobalt-chromium alloy surfaces without polymeric carriers.
abstract: Polymer-based carriers are commonly used to deliver drugs from stents. However, adverse responses to polymer coatings have raised serious concerns. This research is focused on delivering drugs from stents without using polymers or any carriers. Paclitaxel (PAT), an anti-restenotic drug, has strong adhesion towards a variety of material surfaces. In this study, we have utilized such natural adhesion property of PAT to attach these molecules directly to cobalt-chromium (Co-Cr) alloy, an ultra-thin stent strut material. Four different groups of drug coated specimens were prepared by directly adding PAT to Co-Cr alloy surfaces: Group-A (PAT coated, unheated, and ethanol cleaned); Group-B (PAT coated, heat treated, and ethanol cleaned); Group-C (PAT coated, unheated, and not ethanol cleaned); and Group-D (PAT coated, heat treated and not ethanol cleaned). In vitro drug release of these specimens was investigated using high performance liquid chromatography. Groups A and B showed sustained PAT release for up to 56 days. A simple ethanol cleaning procedure after PAT deposition can remove the loosely bound drug crystals from the alloy surfaces and thereby allowing the remaining strongly bound drug molecules to be released at a sustained rate. The heat treatment after PAT coating further improved the stability of PAT on Co-Cr alloy and allowed the drug to be delivered at a much slower rate, especially during the initial 7 days. The specimens which were not cleaned in ethanol, Groups C and D, showed burst release. PAT coated Co-Cr alloy specimens were thoroughly characterized using scanning electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy. These techniques were collectively useful in studying the morphology, distribution, and attachment of PAT molecules on Co-Cr alloy surfaces. Thus, this study suggests the potential for delivering paclitaxel from Co-Cr alloy surfaces without using any carriers.
authors.affiliation: Department of Biomedical Engineering, College of Engineering, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249-0619, USA. Gopinath.Mani@utsa.edu
authors.forename: Gopinath
authors.initials: G
authors.lastname: Mani
authors.forename: Celia E
authors.initials: CE
authors.lastname: Macias
authors.forename: Marc D
authors.initials: MD
authors.lastname: Feldman
authors.forename: Denes
authors.initials: D
authors.lastname: Marton
authors.forename: Sunho
authors.initials: S
authors.lastname: Oh
authors.forename: C
authors.initials: C
authors.lastname: Mauli Agrawal
mesh_terms: D002858:Chromium Alloys / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D017239:Paclitaxel / Q000494:pharmacology*; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors
chemical_list: D002858:Chromium Alloys; D004337:Drug Carriers; D011108:Polymers; D017239:Paclitaxel
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