title: Anti-amyloidogenic activity of IgGs contained in normal plasma.
abstract: BACKGROUND
  We have previously shown that a subpopulation of naturally occurring human IgGs has therapeutic potential for the amyloid-associated disorders. These molecules cross-react with conformational epitopes on amyloidogenic assemblies, including amyloid beta (Abeta) protein fibrils that are a pathological hallmark of Alzheimer's disease.
  
  
  METHODS
  Using our europium-linked immunosorbant assay, we established that approximately 95% of 260 screened donor plasma samples had amyloid fibril-reactive IgGs and Abeta conformer-reactive IgGs with minimal binding to Abeta monomers. Anti-amyloidogenic reactivity was diverse and attributed to Abeta targeting multiple fibril-related binding sites and/or variations in multidentate binding.
  
  
  CONCLUSIONS
  There was no correlation between anti-fibril and anti-oligomer reactivity and donor age (19 to 60 years old) or gender. These findings demonstrate the inherent but diverse anti-amyloidogenic activity of natural IgGs contained in normal plasma.
  
  
  CONCLUSIONS
  Our studies provide support for investigating the clinical significance and physiological function of this novel class of antibodies.
authors.affiliation: Human Immunology and Cancer Program, Department of Medicine, University of Tennessee Graduate School of Medicine, Knoxville, TN 37920, USA. brian.onuallain@ucd.ie
authors.forename: Brian
authors.initials: B
authors.lastname: O'Nuallain
authors.forename: Angela D
authors.initials: AD
authors.lastname: Williams
authors.forename: Helen P
authors.initials: HP
authors.lastname: McWilliams-Koeppen
authors.forename: Luis
authors.initials: L
authors.lastname: Acero
authors.forename: Alfred
authors.initials: A
authors.lastname: Weber
authors.forename: Hartmut
authors.initials: H
authors.lastname: Ehrlich
authors.forename: Hans P
authors.initials: HP
authors.lastname: Schwarz
authors.forename: Alan
authors.initials: A
authors.lastname: Solomon
mesh_terms: D000328:Adult; D000544:Alzheimer Disease / Q000276:immunology / Q000628:therapy; D016229:Amyloid beta-Peptides / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D016756:Immunoglobulins, Intravenous / Q000276:immunology / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000276:immunology*; D055815:Young Adult
chemical_list: D016229:Amyloid beta-Peptides; D000939:Epitopes; D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42)
references.citation: Neurology. 2005 May 10;64(9):1553-62
references.pmid: 15883316
references.citation: J Neurosci Res. 2006 Aug 1;84(2):434-43
references.pmid: 16767774
references.citation: Neurosci Lett. 2004 Jan 30;355(3):226-30
references.pmid: 14732472
references.citation: Biochim Biophys Acta. 2004 Dec 24;1739(1):5-25
references.pmid: 15607113
references.citation: J Immunol. 2006 Jun 1;176(11):7071-8
references.pmid: 16709869
references.citation: Neurobiol Aging. 2004 Sep;25(8):1023-32
references.pmid: 15212827
references.citation: J Neurochem. 2007 Jun;101(5):1172-84
references.pmid: 17286590
references.citation: Biochemistry. 2008 Nov 25;47(47):12254-6
references.pmid: 18956886
references.citation: Curr Alzheimer Res. 2004 Aug;1(3):149-63
references.pmid: 15975063
references.citation: J Neurosci. 2008 Apr 16;28(16):4231-7
references.pmid: 18417702
references.citation: Biochem Biophys Res Commun. 1999 Apr 21;257(3):787-91
references.pmid: 10208861
references.citation: J Neurol Neurosurg Psychiatry. 2004 Oct;75(10):1472-4
references.pmid: 15377700
references.citation: Neurobiol Aging. 2009 Nov;30(11):1728-36
references.pmid: 18294736
references.citation: Cancer Biother Radiopharm. 2003 Dec;18(6):853-60
references.pmid: 14969598
references.citation: BMC Neurosci. 2007 Mar 20;8:22
references.pmid: 17374155
references.citation: Adv Intern Med. 2000;45:107-37
references.pmid: 10635047
references.citation: J Biol Chem. 2005 Apr 29;280(17):17458-63
references.pmid: 15728175
references.citation: Nature. 1992 Sep 24;359(6393):322-5
references.pmid: 1383826
references.citation: Brain Res. 2008 Jul 11;1219:169-79
references.pmid: 18534566
references.citation: Brain. 2003 Sep;126(Pt 9):1935-9
references.pmid: 12821522