Early effects of low dos C ion or X-ray irradiation on human peripheral blood lymphocytes.
The aim of this study was to estimate the acute effects of low dose (12)C(6+) ions or X-ray radiation on human immune function. The human peripheral blood lymphocytes (HPBL) of seven healthy donors were exposed to 0.05Gy (12)C(6+) ions or X-ray radiation and cell responses were measured at 24 hours after exposure. The cytotoxic activities of HPBL were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT); the percentages of T and NK cells subsets were detected by flow cytometry; mRNA expression of interleukin (IL)-2, tumor necrosis factor (TNF)-α and interferon (IFN)-γ were examined by real time quantitative RT-PCR (qRT-PCR); and these cytokines protein levels in supernatant of cultured cells were assayed by enzyme-linked immunosorbent assays (ELISA). The results showed that the cytotoxic activity of HPBL, mRNA expression of IL-2, IFN-γ and TNF-α in HPBL and their protein levels in supernatant were significantly increased at 24 hours after exposure to 0.05Gy (12)C(6+) ions radiation and the effects were stronger than observed for X-ray exposure. However, there was no significant change in the percentage of T and NK cells subsets of HPBL. These results suggested that 0.05Gy high linear energy transfer (LET) (12)C(6+) radiation was a more effective approach to host immune enhancement than that of low LET X-ray. We conclude that cytokines production might be used as sensitive indicators of acute response to LDI.
Chen
Yingtai
Y
College of Clinical Medicine of Lanzhou University. 222 Tianshui Nan Road, Lanzhou, Gansu 730000, People's Republic of China.
Li
Yumin
Y
Zhang
Hong
H
Xie
Yi
Y
Chen
Xuezhong
X
Ren
Jinyu
J
Zhang
Xiaowei
X
Zhu
Zijiang
Z
Liu
Hongliang
H
Zhang
Yawei
Y
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