Antioxidative and immunoprotective effects of Pyracantha fortuneana (Maxim.) Li polysaccharides in mice.
This study was undertaken to determine the effects of Pyracantha fortuneana (Maxim.) Li polysaccharides (PFP) on antioxidant and immune functions in mice. Results from this study showed that PFP administration significantly increased thymus and spleen indices, promoted splenocyte proliferation and natural killer (NK) cell activity, and elevated CD4 T cell numbers as well as CD4(+)/CD8(+) ratios. PFP also increased interleukin-2 (IL-2) levels, and decreased the levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in splenocytes. In addition, PFP treatment led to remarkable increases in glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) activities, and dramatic decreases in malondialdehyde (MDA) levels in splenocytes. Moreover, PFP increased mRNA and protein expression of nuclear factor erythroid 2-related factor (Nrf2) in splenocytes. Taken together, these results suggest that PFP treatment enhances the immune function and decreases the oxidative stress in mice.
Yuan
Chengfu
C
Molecular Oncology and Epigenetics Laboratory, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, People's Republic of China. yuancf46@163.com
Wang
Changdong
C
Bu
Youquan
Y
Xiang
Tingxiu
T
Huang
Xiuning
X
Wang
Zhiwei
Z
Yi
Faping
F
Ren
Guosheng
G
Liu
Geli
G
Song
Fangzhou
F
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005638:Fruit; D005979:Glutathione Peroxidase / Q000378:metabolism; D007109:Immunity / Q000187:drug effects; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011134:Polysaccharides / Q000008:administration & dosage*; D031991:Pyracantha; D013154:Spleen / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology
D016207:Cytokines; D051267:NF-E2-Related Factor 2; D011134:Polysaccharides; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase