Modulating numbers and phenotype of CD8+ T cells in secondary immune responses.
Prime-boost regimens are frequently used to increase the number of memory CD8(+) T cells and thus the protective capacity of experimental vaccinations; however, it is currently unknown how the frequency and phenotype of primary (1 degrees ) memory CD8(+) T cells impact the quantity and phenotype of secondary (2 degrees ) memory CD8(+) T-cell populations. Here, we show that 2 degrees infections of mice that received different 1 degrees infections and/or immunizations generated similar numbers of 2 degrees effector and memory CD8(+) T cells. Remarkably, this result was independent of the numbers and phenotype of 1 degrees memory CD8(+) T cells present at the time of rechallenge. However, after adoptive transfer of low numbers of 1 degrees memory CD8(+) T cells, a linear correlation between 1 degrees memory CD8(+) T-cell input and 2 degrees memory CD8(+) T-cell numbers was observed. These data suggest that, above a very low threshold, boosting of 1 degrees memory CD8(+) T-cell populations elicits 2 degrees immune responses of similar magnitude. Therefore, our study has important implications for the design of prime-boost regimens that aim to generate protective CD8(+) T-cell-mediated immunity.
Wirth
Thomas C
TC
Department of Microbiology, University of Iowa, Iowa City, IA, USA.
Harty
John T
JT
Badovinac
Vladimir P
VP
D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D002452:Cell Count; D002478:Cells, Cultured; D007117:Immunization, Secondary; D007156:Immunologic Memory; D008088:Listeriosis / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology
D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D010047:Ovalbumin
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