title: Mechanisms of the protective effects of BMSCs promoted by TMDR with heparinized bFGF-incorporated stent in pig model of acute myocardial ischemia.
abstract: This study investigates the mechanism by which transmyocardial drilling revascularization combined with heparinized basic fibroblast growth factor incorporated degradable stent implantation (TMDRSI) enhanced effects of bone marrow mesenchymal stem cells (BMSCs) transplantation against acute ischemic myocardial injury. After the mid-third of left anterior descending artery was ligated, miniswine were divided into none-treatment group (control, n = 6), BMSCs implantation group (C, n = 6), TMDRSI group (TS, n = 6) and TMDRSI and BMSCs implantation group (TSC, n = 6). Two channels of 3.5 mm diameter were established by a self-made drill in the ischemic region, into which a stent was implanted for the TS and TSC groups. Autologous BMSCs were transplanted into the ischemic region in C group or around the channels in TSC group. Expression of von Willebrand factor, vascular endothelial growth factor, interleukin-1β, transforming growth factor-β(3) , cell proliferation and apoptosis, histological and morphological analyses, myocardial remodelling and cardiac function were evaluated at different time-points. Six weeks after the operation, the above indices were significantly improved in TSC group compared with others (P < 0.05), though C and TS groups also showed better results than the control group (P < 0.05). The new method was shown to have activated paracrine pathway of transplanted BMSCs, increased survival and differentiation of such cells, and enhanced effects of BMSCs transplantation on myocardial remodelling, which may provide a new strategy for cell therapies against acute ischemic myocardial injury.
authors.affiliation: Department of Surgery, Tianjin Medical University Cardiovascular Clinical College & TEDA International Cardiovascular Hospital, Tianjin, China.
authors.forename: Guang-Wei
authors.initials: GW
authors.lastname: Zhang
authors.forename: Xiao-Cheng
authors.initials: XC
authors.lastname: Liu
authors.forename: Jesse
authors.initials: J
authors.lastname: Li-Ling
authors.forename: Yun
authors.initials: Y
authors.lastname: Luan
authors.forename: Yuan-Ning
authors.initials: YN
authors.lastname: Ying
authors.forename: Xue-Sheng
authors.initials: XS
authors.lastname: Wu
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Zhao
authors.forename: Tian-Jun
authors.initials: TJ
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Lü
mesh_terms: D054058:Acute Coronary Syndrome / Q000473:pathology / Q000628:therapy*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D006493:Heparin / Q000627:therapeutic use; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D009204:Myocardial Revascularization; D015607:Stents; D013552:Swine; D020257:Ventricular Remodeling / Q000187:drug effects
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D016222:Fibroblast Growth Factor 2; D006493:Heparin
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