The genetics of barley low-tillering mutants: low number of tillers-1 (lnt1).
Barley (Hordeum vulgare L.) carrying recessive mutations at the Low number of tillers1 (Lnt1) gene does not develop secondary tillers and only develops one to four tillers by maturity. Double mutant analysis determined that the lnt1 mutant was epistatic to five of the six low and high tillering mutants tested. Double mutants of lnt1 and the low tillering mutant intermedium-b (int-b) resulted in a uniculm plant, indicating a synergistic interaction and that Lnt and Int-b function in separate tillering pathways. RNA profiling identified 70 transcripts with either increased or decreased abundance in the lnt1 mutant compared to wild-type. One gene with reduced transcript levels in the lnt1 mutant was the BELL-like homeodomain transcription factor JuBel2. The JuBel2 allele in the lnt1.a mutant contained a frameshift mutation that eliminated most of the predicted polypeptide, indicating that the Lnt1 gene encodes JuBel2. Previous studies with the low-tillering mutant absent lower laterals (als) showed that the tillering phenotypes and genetic interactions of als and lnt1 with other tillering mutants were very similar. However, the transcriptomes were very different; many transcripts annotated as stress and defense response exhibited increased abundance in the als mutant. This difference suggests a functional separation between Als and Lnt1 in the genetic control of tillering.
Dabbert
Timothy
T
Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108, USA.
Okagaki
Ron J
RJ
Cho
Seungho
S
Heinen
Shane
S
Boddu
Jayanand
J
Muehlbauer
Gary J
GJ
D001483:Base Sequence; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D001467:Hordeum / Q000033:anatomy & histology* / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018520:Plant Shoots / Q000166:cytology / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D010940:Plant Proteins; D012333:RNA, Messenger
BMC Genomics. 2009 Dec 04;10:582
19961604
Plant Physiol. 2004 Mar;134(3):960-8
15020760
Plant J. 2009 May;58(4):641-54
19175771
Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):290-5
9874811
Nature. 1997 Apr 3;386(6624):485-8
9087405
Cell. 1997 May 16;89(4):575-85
9160749
Curr Opin Plant Biol. 2005 Oct;8(5):506-11
16054429
Cell. 1989 Jan 27;56(2):225-9
2643476
Curr Biol. 2004 Jul 27;14(14):1232-8
15268852
Development. 2003 Sep;130(17):3941-50
12874117
Mol Cells. 2006 Feb 28;21(1):147-52
16511358
Development. 2002 Mar;129(5):1131-41
11874909
Genome. 1996 Oct;39(5):866-73
8890517
Plant Physiol. 2009 Jan;149(1):46-55
19126694
Nature. 2003 Apr 10;422(6932):618-21
12687001
Methods Mol Biol. 2000;132:365-86
10547847
Science. 2006 Jun 2;312(5778):1392-6
16614172
Plant Cell. 2003 Aug;15(8):1717-27
12897247
Curr Biol. 2003 Sep 16;13(18):1630-5
13678595
Plant J. 2007 Sep;51(6):1019-29
17655651
Genes Dev. 2003 May 1;17(9):1175-87
12730136
Genes Dev. 2003 Jun 15;17(12):1469-74
12815068
Theor Appl Genet. 2003 Mar;106(5):846-57
12647059
Theor Appl Genet. 2009 May;118(7):1351-60
19241055
Plant J. 2003 Feb;33(3):513-20
12581309
Plant Cell. 2007 Feb;19(2):458-72
17307924
Plant Mol Biol. 2006 Apr;60(6):843-54
16724256
Plant Cell. 2006 Mar;18(3):586-97
16461581
Plant Physiol. 2009 May;150(1):482-93
19321710
Curr Opin Plant Biol. 2007 Feb;10(1):21-5
17140840
Plant J. 2006 Dec;48(5):687-98
17092317
Nucleic Acids Res. 2006 Mar 02;34(4):1281-92
16513846
Mamm Genome. 2001 Dec;12(12):930-2
11707780
Annu Rev Plant Biol. 2008;59:253-79
18444901
Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11765-70
13130077
Plant Cell. 2005 Mar;17(3):746-59
15705953
Plant J. 2001 Jul;27(1):13-23
11489179
Plant Cell. 2009 Apr;21(4):1095-108
19346465
Plant Cell Physiol. 2005 Jan;46(1):79-86
15659436
Dev Biol. 2000 Feb 15;218(2):341-53
10656774
Mol Plant Microbe Interact. 2006 Apr;19(4):407-17
16610744
Plant Physiol. 2006 Nov;142(3):1014-26
16980559