title: [Polymorphisms of vascular diseases-related genes in Guangzhou Biobank Cohort Study].
abstract: OBJECTIVE
  To study the polymorphisms of cerebrovascular and cardiovascular disease genes using Taqman single nucleotide polymorphism (SNP) genotyping kits.
  
  
  METHODS
  A total of 2000 subjects were recruited from the Guangzhou Biobank Cohort Study (GBCS), and 15 SNPs were detected using Taqman SNP genotyping kits and an ABI 7900HT real time PCR system. The data were tested for the Hardy-Weinberg equilibrium, and then compared with the data of the Chinese population from the International HapMap Project (HapMap_HCN).
  
  
  RESULTS
  (1) All genotype data of the 15 SNPs were consistent with the Hardy-Weinberg rules. (2) The significant differences were observed among two SNPs, rs4220 and rs5368 and the HapMap_HCN (rs4220 28.2% vs 17.8%; chi(2) = 4.891, P = 0.028; rs5368 22.1% vs 32.2%, chi(2) = 5.137, P = 0.024). Comparing other gene bank data, such as AFD-CHN-PANEL, the Allele Frequency Database (ALFRED) and JBIC-allele, it would be most likely that our observations represent differences between the Northern and Southern populations in China.
  
  
  CONCLUSIONS
  Such Biobank study provided a useful platform for the study of the role of genetic and environmental determinants on cerebrovascular and cardiovascular disease.
authors.affiliation: Molecular Epidemiological Research Center, Guangzhou No.12 Hospital, Guangzhou 510620, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Chao-Qiang
authors.initials: CQ
authors.lastname: Jiang
authors.forename: Graham Neil
authors.initials: GN
authors.lastname: Thomas
authors.forename: Xiang-Qian
authors.initials: XQ
authors.lastname: Lao
authors.forename: Jie-Ming
authors.initials: JM
authors.lastname: Lin
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Yue
authors.forename: Wei-Sen
authors.initials: WS
authors.lastname: Zhang
authors.forename: Tong
authors.initials: T
authors.lastname: Zhu
authors.forename: Da-Qing
authors.initials: DQ
authors.lastname: Lin
mesh_terms: D044466:Asian People / Q000235:genetics; D018070:Biological Specimen Banks / Q000706:statistics & numerical data*; D001927:Brain Diseases / Q000453:epidemiology / Q000235:genetics*; D002318:Cardiovascular Diseases / Q000453:epidemiology / Q000235:genetics*; D002681:China / Q000453:epidemiology; D015331:Cohort Studies; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide