title: Small RNA cloning.
abstract: The next generation sequencing technologies have made the exhaustive sequencing of all small RNA species within a small RNA library possible. Thus, comparative studies on the changes in small-RNA transcriptomes between two biological samples represent a powerful means of revealing the functions of small RNAs in physiological and pathological processes. Here, we describe a small RNA cloning method that can be used to generate small RNA cDNA libraries for both conventional and next generation (deep) sequencing. It can also be used for detection and quantitative analyses of small RNAs using PCR.
authors.affiliation: Department of Physiology, Anderson Biomedical Science, University of Nevada School of Medicine, Reno, NV, USA.
authors.forename: Seungil
authors.initials: S
authors.lastname: Ro
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D026723:Polyadenylation / Q000235:genetics; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000235:genetics* / Q000302:isolation & purification; D048348:Reverse Transcription / Q000235:genetics; D017423:Sequence Analysis, RNA; D013698:Templates, Genetic; D014170:Transformation, Genetic
chemical_list: D034741:RNA, Small Interfering
references.citation: Trends Genet. 2006 Mar;22(3):165-73
references.pmid: 16446010
references.citation: Science. 2001 Oct 26;294(5543):862-4
references.pmid: 11679672
references.citation: Dev Cell. 2003 Aug;5(2):337-50
references.pmid: 12919683
references.citation: Science. 2006 Jul 21;313(5785):363-7
references.pmid: 16778019
references.citation: RNA. 2007 Dec;13(12):2366-80
references.pmid: 17951331
references.citation: Genes Dev. 2006 Aug 1;20(15):1993-7
references.pmid: 16882976
references.citation: Science. 2001 Oct 26;294(5543):858-62
references.pmid: 11679671
references.citation: Science. 2006 Jul 21;313(5785):320-4
references.pmid: 16809489
references.citation: RNA. 2007 Oct;13(10):1693-702
references.pmid: 17698640
references.citation: Dev Biol. 2007 Nov 15;311(2):592-602
references.pmid: 17936267
references.citation: Gene Expr. 2002;10(1-2):17-39
references.pmid: 11868985
references.citation: Nature. 2006 Jul 13;442(7099):199-202
references.pmid: 16751776
references.citation: FEBS Lett. 2009 Jun 5;583(11):1668-73
references.pmid: 19379744
references.citation: Biochem Biophys Res Commun. 2006 Dec 22;351(3):756-63
references.pmid: 17084816
references.citation: Cell. 2006 Dec 15;127(6):1193-207
references.pmid: 17174894
references.citation: Nature. 2006 Jul 13;442(7099):203-7
references.pmid: 16751777
references.citation: Genes Dev. 2006 Jul 1;20(13):1709-14
references.pmid: 16766680
references.citation: Science. 2001 Oct 26;294(5543):853-8
references.pmid: 11679670
references.citation: ScientificWorldJournal. 2006 Mar 26;6:1828-40
references.pmid: 17205190
references.citation: Genes Dev. 2006 Aug 15;20(16):2214-22
references.pmid: 16882972
references.citation: Genes Dev. 2006 Jul 1;20(13):1732-43
references.pmid: 16766679
references.citation: J Biomed Biotechnol. 2006;2006(4):69616
references.pmid: 17057368