Improved tolerance toward fungal diseases in transgenic Cavendish banana (Musa spp. AAA group) cv. Grand Nain.
The most devastating disease currently threatening to destroy the banana industry worldwide is undoubtedly Sigatoka Leaf spot disease caused by Mycosphaerella fijiensis. In this study, we developed a transformation system for banana and expressed the endochitinase gene ThEn-42 from Trichoderma harzianum together with the grape stilbene synthase (StSy) gene in transgenic banana plants under the control of the 35S promoter and the inducible PR-10 promoter, respectively. The superoxide dismutase gene Cu,Zn-SOD from tomato, under control of the ubiquitin promoter, was added to this cassette to improve scavenging of free radicals generated during fungal attack. A 4-year field trial demonstrated several transgenic banana lines with improved tolerance to Sigatoka. As the genes conferring Sigatoka tolerance may have a wide range of anti-fungal activities we also inoculated the regenerated banana plants with Botrytis cinerea. The best transgenic lines exhibiting Sigatoka tolerance were also found to have tolerance to B. cinerea in laboratory assays.
Vishnevetsky
Jane
J
Department of Fruit Tree Sciences, Institute of Plant Sciences, The Agricultural Research Organization, P.O. Box 6, 50250, Bet-Dagan, Israel.
White
Thomas L
TL
Palmateer
Aaron J
AJ
Flaishman
Moshe
M
Cohen
Yuval
Y
Elad
Yigal
Y
Velcheva
Margarita
M
Hanania
Uri
U
Sahar
Nachman
N
Dgani
Oded
O
Perl
Avihai
A
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D001203:Ascomycota / Q000145:classification / Q000472:pathogenicity*; D020171:Botrytis / Q000145:classification / Q000472:pathogenicity*; D002688:Chitinases / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000201:enzymology / Q000235:genetics; D028521:Musa / Q000201:enzymology* / Q000235:genetics* / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics; D027843:Vitis / Q000201:enzymology / Q000235:genetics
D013482:Superoxide Dismutase; D000217:Acyltransferases; C022674:stilbene synthase; D002688:Chitinases
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