title: IgA detection in human neurocysticercosis using different preparations of heterologous antigen.
abstract: Neurocysticercosis (NC) is the most important neurological disease of parasitic origin in humans. IgA and IgG detection in serum from neurocysticercosis patients was tested using some antigenic preparations of total saline extract from Taenia saginata: detergent (D) and aqueous (A) phases extracted with Triton X-114 and the jacalin bound (JBF) and unbound fractions (JUF) obtained by affinity chromatography using jacalin column. Samples were obtained from 45 patients with definitive NC, who were subdivided into active-NC group and inactive-NC group; 35 patients with other parasitoses; and 30 apparently healthy individuals. Sensitivity and specificity were calculated. Specificity to detect IgA and IgG for D phase, respectively, were 89.8% and 86.9% and for IgG detection 91.3% and 76.8% when using D phase and JUF, respectively. D phase and JBF proved to be specific and efficient and could be efficiently utilized as an alternative antigen for IgA detection in NC, with comparable results with IgG.
authors.affiliation: Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará 1720, CEP 38400-902, Uberlândia, Minas Gerais, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: da S Ribeiro
authors.forename: Marianna N
authors.initials: MN
authors.lastname: Manhani
authors.forename: Julia M
authors.initials: JM
authors.lastname: Costa-Cruz
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000302:isolation & purification; D015478:Antigens, Heterophile / Q000302:isolation & purification; D002458:Cell Fractionation; D002846:Chromatography, Affinity; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology; D010274:Parasitology / Q000379:methods*; D012680:Sensitivity and Specificity; D041202:Taenia saginata / Q000737:chemistry* / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015478:Antigens, Heterophile; D007070:Immunoglobulin A; D007074:Immunoglobulin G
references.citation: Mem Inst Oswaldo Cruz. 1996 Jan-Feb;91(1):63-70
references.pmid: 8734950
references.citation: Neurocirugia (Astur). 2007 Apr;18(2):101-10
references.pmid: 17497055
references.citation: Arq Neuropsiquiatr. 2000 Mar;58(1):18-24
references.pmid: 10770861
references.citation: Trends Immunol. 2001 Apr;22(4):205-11
references.pmid: 11274926
references.citation: Clin Immunol. 2005 Jul;116(1):3-10
references.pmid: 15925826
references.citation: Parasitol Res. 2007 Sep;101(4):1007-13
references.pmid: 17510761
references.citation: Arq Neuropsiquiatr. 2002 Jun;60(2-B):400-5
references.pmid: 12131940
references.citation: Arch Intern Med. 1985 Mar;145(3):442-5
references.pmid: 3977513
references.citation: Trop Med Int Health. 2000 Jun;5(6):443-9
references.pmid: 10929145
references.citation: Rev Inst Med Trop Sao Paulo. 2006 Nov-Dec;48(6):343-6
references.pmid: 17221132
references.citation: J Clin Microbiol. 1986 Oct;24(4):536-41
references.pmid: 3771742
references.citation: J Biol Chem. 1951 Nov;193(1):265-75
references.pmid: 14907713
references.citation: Rev Soc Bras Med Trop. 2008 Mar-Apr;41(2):135-41
references.pmid: 18545832
references.citation: J Neurol Sci. 1996 Oct;142(1-2):1-6
references.pmid: 8902711
references.citation: Parasitol Res. 2008 Apr;102(5):1097-101
references.pmid: 18322701
references.citation: Trop Med Int Health. 2007 Nov;12(11):1369-76
references.pmid: 17949403
references.citation: Parasitol Int. 2006;55 Suppl:S69-73
references.pmid: 16352461
references.citation: Parasitol Res. 1987;73(3):265-70
references.pmid: 3495795
references.citation: Rev Inst Med Trop Sao Paulo. 1982 Nov-Dec;24(6):337-41
references.pmid: 6763759
references.citation: Acta Trop. 2008 Jun;106(3):168-74
references.pmid: 18462701
references.citation: Indian Pediatr. 2006 Mar;43(3):227-35
references.pmid: 16585817
references.citation: Lancet. 2003 Aug 16;362(9383):547-56
references.pmid: 12932389