[Cloning and expression of SjMRLC and analysis of its stage-specific transcription].
OBJECTIVE
To clone and express Schistosoma japonicum myosin regulatory light chain (SjMRLC) in prokaryotic expression system and analyze its stage-specific transcription.


METHODS
The recombinant plasmid was constructed with pGEX 4T-3 vector and expressed in BL21. The recombinant SjMRLC was identified through Western blotting assay. Total RNA from different stages of Schistosoma japonicum was extracted and used to detect the stage-specific transcription of SjMRLC.


RESULTS
The recombinant plasmid of pGEX 4T-3/SjMRLC was constructed correctly. The recombinant protein (rSjMRLC) with GST tag was soluble. SjMRLC showed similar transcription level in all stages including sporocyst, miracidium, egg, cercaria, schistosomulum, female worm, male worm, and paired adults.


CONCLUSIONS
The soluble recombinant protein SjMRLC with GST tag was obtained. SjMRLC is a house-keeping gene.
Qian
Men-bao
MB
National Institute of Parasitic Diseases, Chinese Centre for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, MOH, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
Xu
Bin
B
Kong
Juan
J
Ju
Chuan
C
Feng
Zheng
Z
Hu
Wei
W
D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D018994:Myosin Light Chains / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012549:Schistosoma japonicum / Q000235:genetics* / Q000378:metabolism
D015801:Helminth Proteins; D018994:Myosin Light Chains; D011994:Recombinant Proteins