title: Temporal and spatial regulation of miR-320 in the uterus during embryo implantation in the rat.
abstract: The implantation process is complex, requiring reciprocal interactions between implantation-competent blastocysts and the receptive uterus. There were reports to show that some microRNAs (miRNAs) may play a key role during embryo implantation in mouse. However, the miR-320 expression profiles in the rat uterus during peri-implantation are unknown. In the present study, we found that the expression level of miR-320 was lower on day 5 of gestation (g.d. 5) in rats than g.d.3 and g.d.4 and restored gradually from g.d.6. MiR-320 was specifically localized in glandular and luminal epithelia and decidua. The expression of miR-320 was not significantly different in the pseudopregnant uterus and decreased in the uteri of rats subjected to activation of delayed implantation. Artificial decidualization and treatment with progesterone increased the miR-320 expression. Thus, miR-320 was differentially expressed in the rat uterus during implantation. The expression level was affected by active blastocysts and decidualization during the window of implantation. Steroid hormones, progesterone stimulated miR-320 expression.
authors.affiliation: Reproductive and Genetic Center, National Research Institute for Family Planning, Beijing 100081, China.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Xia
authors.affiliation: Reproductive and Genetic Center, National Research Institute for Family Planning, Beijing 100081, China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Jin
authors.affiliation: Reproductive and Genetic Center, National Research Institute for Family Planning, Beijing 100081, China.|Graduate School, Peking Union Medical College, Beijing 100005, China.
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Song
authors.affiliation: Reproductive and Genetic Center, National Research Institute for Family Planning, Beijing 100081, China.|Graduate School, Peking Union Medical College, Beijing 100005, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Reproductive and Genetic Center, National Research Institute for Family Planning, Beijing 100081, China.
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Liu
authors.affiliation: Reproductive and Genetic Center, National Research Institute for Family Planning, Beijing 100081, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D003656:Decidua / Q000378:metabolism; D010064:Embryo Implantation / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005865:Gestational Age; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D011372:Progestins / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D011372:Progestins; D011374:Progesterone
references.citation: Hum Reprod. 2006 Sep;21(9):2272-80
references.pmid: 16798842
references.citation: Semin Reprod Endocrinol. 1999;17(3):275-90
references.pmid: 10797946
references.citation: Am J Physiol. 1998 Mar;274(3):E410-6
references.pmid: 9530122
references.citation: Mol Cancer Res. 2003 Oct;1(12):882-91
references.pmid: 14573789
references.citation: RNA. 2008 Nov;14(11):2348-60
references.pmid: 18812439
references.citation: Hum Reprod. 2005 Sep;20(9):2639-47
references.pmid: 15946996
references.citation: Semin Cell Dev Biol. 2008 Apr;19(2):161-9
references.pmid: 18054836
references.citation: Childs Nerv Syst. 2006 Nov;22(11):1419-25
references.pmid: 16983573
references.citation: Cell. 2007 Jun 29;129(7):1401-14
references.pmid: 17604727
references.citation: Cancer Res. 2007 Oct 15;67(20):9762-70
references.pmid: 17942906
references.citation: FEBS J. 2005 Jan;272(1):59-73
references.pmid: 15634332
references.citation: Cancer Res. 2005 Jul 15;65(14):6029-33
references.pmid: 16024602
references.citation: Genes Dev. 2006 Jul 1;20(13):1732-43
references.pmid: 16766679
references.citation: Endocrine. 2003 Jul;21(2):123-32
references.pmid: 12897374
references.citation: J Biol Chem. 2008 Aug 22;283(34):23473-84
references.pmid: 18556655
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20297-302
references.pmid: 19088191
references.citation: Exp Hematol. 2009 Feb;37(2):245-55
references.pmid: 19135902
references.citation: Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):15144-9
references.pmid: 17848513
references.citation: Reprod Fertil Dev. 2007;19(3):510-9
references.pmid: 17394800
references.citation: Endocr Rev. 2004 Jun;25(3):341-73
references.pmid: 15180948
references.citation: Childs Nerv Syst. 2008 Apr;24(4):485-92
references.pmid: 17962954
references.citation: Nat Rev Genet. 2006 Mar;7(3):185-99
references.pmid: 16485018
references.citation: Placenta. 2002 Nov;23(10):763-70
references.pmid: 12398816
references.citation: Biochem Biophys Res Commun. 2006 Oct 13;349(1):236-44
references.pmid: 16935259