title: Phosphorylation of receptor-like cytoplasmic kinases by bacterial flagellin.
abstract: Molecular mechanisms that distinguish self and non-self are fundamental in innate immunity to prevent infections in plants and animals. Recognition of the conserved microbial components triggers immune responses against a broad spectrum of potential pathogens. In Arabidopsis, bacterial flagellin was perceived by a leucine-rich repeat-receptor-like kinase (LRR-RLK) FLS2. Upon flagellin perception, FLS2 forms a complex with another LRR-RLK BAK1. The intracellular signaling events downstream of FLS2/BAK1 receptor complex are still poorly understood. We recently identified a receptor-like cytoplasmic kinase (RLCK) BIK1 that associates with flagellin receptor complex to initiate plant innate immunity. BIK1 is rapidly phosphorylated upon flagellin perception in an FLS2- and BAK1-dependent manner. BAK1 directly phosphorylates BIK1 with an in vitro kinase assay. Plants have evolved a large number of RLCK genes involved in a wide range of biological processes. We provided evidence here that additional RLCKs could also be phosphorylated by flagellin and may play redundant role with BIK1 in plant innate immunity.
authors.affiliation: Department of Plant Pathology and Microbiology (S.W. & L.S.), Department of Biochemistry and Biophysics (D.L & P.H.) and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX USA.
authors.forename: Dongping
authors.initials: D
authors.lastname: Lu
authors.affiliation: Department of Plant Pathology and Microbiology (S.W. & L.S.), Department of Biochemistry and Biophysics (D.L & P.H.) and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX USA.
authors.forename: Shujing
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Plant Pathology and Microbiology (S.W. & L.S.), Department of Biochemistry and Biophysics (D.L & P.H.) and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX USA.
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.affiliation: Department of Plant Pathology and Microbiology (S.W. & L.S.), Department of Biochemistry and Biophysics (D.L & P.H.) and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX USA.
authors.forename: Libo
authors.initials: L
authors.lastname: Shan
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D005408:Flagellin / Q000378:metabolism*; D008954:Models, Biological; D010766:Phosphorylation; D057865:Plant Immunity; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D051192:Receptors, Pattern Recognition / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D051192:Receptors, Pattern Recognition; D005408:Flagellin; D020794:Receptor Protein-Tyrosine Kinases
references.citation: Nature. 2004 Feb 26;427(6977):858-61
references.pmid: 14985766
references.citation: Science. 2009 May 8;324(5928):742-4
references.pmid: 19423812
references.citation: Science. 2008 Jul 25;321(5888):557-60
references.pmid: 18653891
references.citation: Nat Immunol. 2006 Dec;7(12):1243-9
references.pmid: 17110940
references.citation: Cell Microbiol. 2007 Jun;9(6):1385-96
references.pmid: 17451411
references.citation: Cell. 2006 Feb 24;124(4):783-801
references.pmid: 16497588
references.citation: Science. 2003 Aug 29;301(5637):1230-3
references.pmid: 12947197
references.citation: Cell. 2006 Feb 24;124(4):803-14
references.pmid: 16497589
references.citation: Science. 2009 Nov 6;326(5954):850-3
references.pmid: 19892983
references.citation: Nature. 2007 Jul 26;448(7152):497-500
references.pmid: 17625569
references.citation: Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):496-501
references.pmid: 20018686
references.citation: Plant Physiol. 2003 Jun;132(2):530-43
references.pmid: 12805585
references.citation: Genetics. 1999 May;152(1):401-12
references.pmid: 10224270
references.citation: Mol Cell. 2000 Jun;5(6):1003-11
references.pmid: 10911994
references.citation: Plant Cell. 2006 Jan;18(1):257-73
references.pmid: 16339855
references.citation: Plant Cell. 2006 Mar;18(3):764-79
references.pmid: 16461584
references.citation: Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12217-22
references.pmid: 17626179
references.citation: Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10763-8
references.pmid: 11526204
references.citation: Nature. 2006 Nov 16;444(7117):323-9
references.pmid: 17108957
references.citation: Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4584-90
references.pmid: 19264966