title: Estimation of pullulan by hydrolysis with pullulanase.
abstract: A novel method for the estimation of pullulan was developed in which pullulan was hydrolysed by pullulanase. The hydrolysed product was mainly maltotriose and was determined colorimetrically using 3,5-dimethylsalicylic acid. This gave good linearity with respect to the concentration of pullulan in the fermentation broth. The content of pullulan determined in this way was less than that determined by a coupled enzyme assay and was comparable to that determined by an HPLC method. The new method was specific for estimation of pullulan, demonstrated high accuracy, and could assay pullulan from up to 3.2 mg/ml.
authors.affiliation: School of Food Engineering, HuaiHai Institute of Technology, Lianyungang, China.
authors.forename: Sheng-Jun
authors.initials: SJ
authors.lastname: Wu
authors.forename: Jin-Moon
authors.initials: JM
authors.lastname: Kim
authors.forename: Chao
authors.initials: C
authors.lastname: Zhou
authors.forename: Zheng-Yu
authors.initials: ZY
authors.lastname: Jin
authors.forename: Qun-Yi
authors.initials: QY
authors.lastname: Tong
mesh_terms: D002851:Chromatography, High Pressure Liquid; D057075:Enzyme Assays / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D005936:Glucans / Q000032:analysis* / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D015394:Molecular Structure; D012372:ROC Curve; D013213:Starch / Q000737:chemistry
chemical_list: D005936:Glucans; D000431:Ethanol; C009109:pullulan; D013213:Starch; D006026:Glycoside Hydrolases; C100181:pullulanase