Production of a type 2 maternal diabetes rodent model using the combination of high-fat diet and moderate dose of streptozocin.
BACKGROUND
Pregnancy may be complicated by maternal diabetes. The following experiments were performed in an attempt to produce mouse models of insulin-resistant maternal diabetes.


METHODS
CD1 females received 200 mg/kg streptozocin (STZ) to model insulin-dependent diabetes (T1 group). Another group of females (T2 group) was put on a HFD 4 weeks before receiving 100 mg/kg STZ. After 4 additional weeks of HFD, hyperglycemic females were separated and bred. In another experiement, CD1 females were fed a HFD for 4 weeks before receiving an intravenous (GDM1 group) or intraperitoneal (GDM2 group) injection of 100 mg/kg STZ. Females from GDM2 group were bred at the same day of the STZ injection. Females from GDM1 group were bred 4 weeks after the STZ injection.


CONCLUSIONS
About 25% of the females from T2 group became hyperglycemic after 4 weeks of the injection of STZ. Fifty percent of the females from GDM1 group reached hyperglycemic levels greater than 250 mg/dl during pregnancy. The combination of HFD and moderate STZ in CD1 mice therefore produced hyperglycemic females; however numbers of these mice were somewhat low.
Gutierrez
J Claudio
JC
Instituto de Farmacología y Morfofisiología, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile. jcgutierrez@docentes.uach.cl
Bahamonde
Javiera
J
Prater
M Renee
MR
Yefi
Claudia P
CP
Holladay
Steven D
SD
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000209:etiology / Q000378:metabolism*; D016640:Diabetes, Gestational / Q000097:blood / Q000209:etiology / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage*; D004195:Disease Models, Animal; D005260:Female; D007328:Insulin / Q000097:blood; D051379:Mice; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000097:blood / Q000209:etiology / Q000378:metabolism*; D013311:Streptozocin / Q000008:administration & dosage*
D001786:Blood Glucose; D004041:Dietary Fats; D007328:Insulin; D013311:Streptozocin