title: Effect of mineral sulphur availability on nitrogen and sulphur uptake and remobilization during the vegetative growth of Brassica napus L.
abstract: Because it has a high demand for sulphur (S), oilseed rape is particularly sensitive to S limitation. However, the physiological effects of S limitation remain unclear, especially during the rosette stage. For this reason a study was conducted to determine the effects of mineral S limitation on nitrogen (N) and S uptake and remobilization during vegetative growth of oilseed rape at both the whole-plant and leaf rank level for plants grown during 35 d with 300 microM (34)SO(4)(2-) (control plants; +S) or with 15 microM (34)SO(4)(2-) (S-limited plants; -S). The results highlight that S-limited plants showed no significant differences either in whole-plant and leaf biomass or in N uptake, when compared with control plants. However, total S and (34)S (i.e. deriving from S uptake) contents were greatly reduced for the whole plant and leaf after 35 d, and a greater redistribution of endogenous S from leaves to the benefit of roots was observed. The relative expression of tonoplast and plasmalemma sulphate transporters was also strongly induced in the roots. In conclusion, although S-limited plants had 20 times less mineral S than control plants, their development remained surprisingly unchanged. During S limitation, oilseed rape is able to recycle endogenous S compounds (mostly sulphate) from leaves to roots. However, this physiological adaptation may be effective only over a short time scale (i.e. vegetative growth).
authors.affiliation: UMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie et Nutritions N, C, S (EVA), Institut National de la Recherche Agronomique, University of Caen, Caen, France.
authors.forename: M
authors.initials: M
authors.lastname: Abdallah
authors.forename: L
authors.initials: L
authors.lastname: Dubousset
authors.forename: F
authors.initials: F
authors.lastname: Meuriot
authors.forename: P
authors.initials: P
authors.lastname: Etienne
authors.forename: J-C
authors.initials: JC
authors.lastname: Avice
authors.forename: A
authors.initials: A
authors.lastname: Ourry
mesh_terms: D027321:Anion Transport Proteins / Q000235:genetics / Q000378:metabolism; D018533:Biomass; D029688:Brassica napus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008903:Minerals / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism*; D013460:Sulfur Isotopes / Q000378:metabolism
chemical_list: D027321:Anion Transport Proteins; D008903:Minerals; D009587:Nitrogen Isotopes; D013431:Sulfates; D013460:Sulfur Isotopes; D013455:Sulfur; D009584:Nitrogen
references.citation: Plant Cell. 2002 Apr;14(4):805-15
references.pmid: 11971136
references.citation: Plant Physiol. 1996 Apr;110(4):1151-1157
references.pmid: 12226249
references.citation: Plant Physiol. 1997 Dec;115(4):1671-1680
references.pmid: 12223887
references.citation: Plant Cell Environ. 2006 Mar;29(3):382-95
references.pmid: 17080593
references.citation: J Exp Bot. 2006;57(9):1949-56
references.pmid: 16720615
references.citation: Plant Biol (Stuttg). 2007 Sep;9(5):654-61
references.pmid: 17853365
references.citation: Planta. 2004 Sep;219(5):765-72
references.pmid: 15118859
references.citation: J Exp Bot. 2009;60(11):3239-53
references.pmid: 19553370
references.citation: Plant Physiol. 2004 Jan;134(1):388-400
references.pmid: 14671012
references.citation: Plant Mol Biol. 1999 Sep;41(2):195-206
references.pmid: 10579487
references.citation: Ann Bot. 2005 Jun;95(7):1187-98
references.pmid: 15802311
references.citation: Ann Bot. 2005 Apr;95(5):853-61
references.pmid: 15701662
references.citation: Planta. 1999 Sep;209(3):282-9
references.pmid: 10502094
references.citation: Plant Physiol. 1978 Jun;61(6):900-3
references.pmid: 16660422
references.citation: Plant Physiol. 1998 Dec;118(4):1337-44
references.pmid: 9847107
references.citation: Plant Mol Biol. 1998 Jun;37(3):455-69
references.pmid: 9617813
references.citation: Plant Physiol. 1997 Nov;115(3):1231-1239
references.pmid: 12223869
references.citation: Annu Rev Biochem. 1983;52:507-35
references.pmid: 6351728
references.citation: Plant Physiol. 2003 Jun;132(2):597-605
references.pmid: 12805590
references.citation: Plant Physiol. 2008 Aug;147(4):1830-44
references.pmid: 18552235
references.citation: J Exp Bot. 2001 Aug;52(361):1655-63
references.pmid: 11479330
references.citation: J Exp Bot. 2003 Mar;54(384):1045-56
references.pmid: 12598574
references.citation: Plant Biol (Stuttg). 2007 Sep;9(5):647-53
references.pmid: 17853364
references.citation: J Exp Bot. 2001 Jan;52(354):113-21
references.pmid: 11181720
references.citation: Plant Physiol. 1988 Mar;86(3):798-802
references.pmid: 16665991
references.citation: Proc Natl Acad Sci U S A. 1980 Nov;77(11):6670-2
references.pmid: 16592917
references.citation: J Exp Bot. 2004 Aug;55(404):1831-42
references.pmid: 15286142
references.citation: Plant Physiol. 2008 Jun;147(2):897-911
references.pmid: 18400935
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609