Identification of diverse antimicrobial resistance determinants carried on bacterial, plasmid, or viral metagenomes from an activated sludge microbial assemblage.
Using both sequence- and function-based metagenomic approaches, multiple antibiotic resistance determinants were identified within metagenomic libraries constructed from DNA extracted from bacterial chromosomes, plasmids, or viruses within an activated sludge microbial assemblage. Metagenomic clones and a plasmid that in Escherichia coli expressed resistance to chloramphenicol, ampicillin, or kanamycin were isolated, with many cloned DNA sequences lacking any significant homology to known antibiotic resistance determinants.
Parsley
Larissa C
LC
Department of Biological Sciences, Auburn University, 120 W. Samford Ave., Auburn, AL 36849, USA.
Consuegra
Erin J
EJ
Kakirde
Kavita S
KS
Land
Andrew M
AM
Harper
Willie F
WF
Liles
Mark R
MR
D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D007612:Kanamycin / Q000494:pharmacology; D054892:Metagenome; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004279:DNA, Viral; D012722:Sewage; D007612:Kanamycin; D002701:Chloramphenicol; D000667:Ampicillin
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