Invariant Valpha14 natural killer T cell activation by edible mushroom acidic glycosphingolipids.
Invariant natural killer T (iNKT) cells regulate multi-immune response through Th1/Th2 cytokine release triggered by the recognition of CD1d-restricted glycosphingolipid antigens. Here we report that acidic glycosphingolipids (AGLs) of mushroom (Hypsizigus marmoreus and Pleurotus eryngii) presented by murine CD1d-transfected rat basophilic leukocytes induced interleukin-2 (IL-2) release from iNKT hybridoma cells. AGL-1, one of the AGLs, containing mannose at the non-reducing ends, induced CD1d-dependent IL-2 release. Al-though alpha-galactosylceramide (alpha-GalCer) presented by CD11c-positive cells induced both interferon-gamma (IFN-gamma) and IL-4 release, all of AGLs presented by CD11c-positive cells and AGL-1 presented by B cells induced IL-4 release from iNKT hybridoma cells. A single intravenous injection of AGLs into B6 mice induced only a little elevation of IL-4 in serum but repeated intravenous injection of AGLs induced prolonged retention of IL-4 in serum; therefore, these results suggested that edible mushroom AGLs might contribute to the retention of immunohomeostasis through the minimum induction of iNKT cell activation in vivo.
Nozaki
Hirofumi
H
Department of Food Science, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Itonori
Saki
S
Sugita
Mutsumi
M
Nakamura
Kimihide
K
Ohba
Kiyoshi
K
Ohnishi
Masao
M
Imai
Kunitoshi
K
Igarashi
Yasuyuki
Y
Suzuki
Akemi
A
Kushi
Yasunori
Y
D020384:Acidic Glycosphingolipids / Q000302:isolation & purification / Q000494:pharmacology*; D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D055610:Antigens, CD1d / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism; D001491:Basophils / Q000378:metabolism; D001688:Biological Products / Q000494:pharmacology*; D039521:CD11c Antigen / Q000378:metabolism; D005699:Galactosylceramides / Q000378:metabolism; D006825:Hybridomas; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D055611:Natural Killer T-Cells / Q000187:drug effects* / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D016176:T-Lymphocyte Subsets
D020384:Acidic Glycosphingolipids; D000276:Adjuvants, Immunologic; D055610:Antigens, CD1d; D001688:Biological Products; D039521:CD11c Antigen; D005699:Galactosylceramides; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D015847:Interleukin-4; D007371:Interferon-gamma