A colorimetric method for detection of K-ras codon 12 point mutations in DNA extracted from tissue and peripheral blood in pancreatic disorders.
Molecular-based methods to monitor point mutations require special and expensive equipment unavailable in most hospitals. Colorimetric-based analysis is an ideal platform for K-ras codon 12 gene point mutations because it uses commonly found hospital equipment. The colorimetric assay is sensitive and specific, detecting mutated DNA levels as low as 1% in a wild-type background. Paired genomic DNA extracts of fixed tissue and cellular fractions of peripheral blood are more sensitive and accurate than unpaired samplings. This approach has the potential to improve K-ras point mutation scans as well as to detect micrometastases in circulating tumor cells.
Ollar
Robert A
RA
Department of Surgery, St. Vincent's Hospital Manhattan, New York, NY 10011, USA. rao48@yahoo.com
Cooperman
Avram M
AM
Wayne
Michael E
ME
Barrecchia
James F
JF
Sonpal
Niket
N
Duddempudi
Sushil
S
Kasmin
Franklin E
FE
D003062:Codon / Q000235:genetics*; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000097:blood* / Q000235:genetics*; D006801:Humans; D010182:Pancreatic Diseases / Q000097:blood / Q000235:genetics* / Q000473:pathology; D017354:Point Mutation / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins / Q000235:genetics*
D003062:Codon; C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; D004247:DNA; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins