Effect of carbaryl on survival and development in Bombina orientalis (Boulenger) embryos.
Bombina orientalis is one of the most common amphibians in the world and comprise a large proportion of their total number in Korea. B. orientalis, spawns in the farming regions at Spring when the massive application of agricultural chemicals occurs. Carbaryl, carbamate chemical is a slightly to highly toxic insecticide inhibiting acetylcholinesterase. The embryotoxicity and teratogenic effects of carbaryl on B. orientalis embryos were investigated at 5, 10, 50 and 100 muM. The survival rates of embryos at 312 h post fertilization were decreased with concentration dependent manner. Exposure to carbaryl produced 4 types of severe external abnormalities such as bent trunk, thick-set body, bent tail and ventral blister. At 5 muM carbaryl, a dose of no observed effect on embryonic survival, developmental abnormalities were significantly increased. The developmental abnormalities showed in order of frequency with bent trunk, thick-set body, bent tail and ventral blister. This result suggests that carbaryl is detrimental for embryonic survival and teratogenic by causing the axial skeletal defects in B. orientalis embryos.
Kang
Han Seung
HS
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
Park
Chan Jin
CJ
Gye
Myung Chan
MC
D000014:Abnormalities, Drug-Induced / Q000209:etiology*; D000818:Animals; D001001:Anura / Q000196:embryology* / Q000502:physiology; D012721:Carbaryl / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000196:embryology; D005260:Female; D007306:Insecticides / Q000633:toxicity*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D008136:Longevity / Q000187:drug effects; D008297:Male; D013723:Teratogens / Q000633:toxicity*
D007306:Insecticides; D013723:Teratogens; D012721:Carbaryl