Cinnamaldehyde up-regulates the mRNA expression level of TRPV1 receptor potential ion channel protein and its function in primary rat DRG neurons in vitro.
Cinnamaldehyde (1) is a pharmacologically active ingredient isolated from cassia twig (Ramulus Cinnamomi), which is commonly used in herbal remedies to treat fever-related diseases. Both TRPV1 and TRPM8 ion channel proteins are abundantly expressed in sensory neurons, and are assumed to act as a thermosensor, with the former mediating the feeling of warmth and the latter the feeling of cold in the body. Both of them have recently been reported to be involved in thermoregulation. The purpose of this paper is to further uncover the antipyretic mechanisms of 1 by investigating its effects on the mRNA expression levels and functions of both TRPV1 and TRPM8. The results showed that 1 could up-regulate the mRNA expression levels of TRPV1 at both 37 and 39 degrees C, and its calcium-mediating function was significantly increased at 39 degrees C, all of which could not be blocked by pretreatment of the neuronal cells with ruthenium red, a general transient receptor potential (TRP) blocker, indicating that the action of 1 was achieved through a non-TRPA1 channel pathway. In conclusion, the findings in our in vitro studies might account for part of the peripheral molecular mechanisms for the antipyretic action of 1.
Sui
Feng
F
Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Lin
Na
N
Guo
Jian-You
JY
Zhang
Chang-Bin
CB
Du
Xin-Liang
XL
Zhao
Bao-Sheng
BS
Liu
Hong-Bin
HB
Yang
Na
N
Li
Lan-Fang
LF
Guo
Shu-Ying
SY
Huo
Hai-Ru
HR
Jiang
Ting-Liang
TL
D000171:Acrolein / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002211:Capsaicin / Q000494:pharmacology; D002366:Cassia / Q000737:chemistry*; D007473:Ion Channels / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D018547:Plant Stems / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D050053:TRPM Cation Channels / Q000378:metabolism; D050916:TRPV Cation Channels / Q000235:genetics* / Q000378:metabolism
D007473:Ion Channels; D012333:RNA, Messenger; D050053:TRPM Cation Channels; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; C498947:Trpm8 protein, rat; D000171:Acrolein; D002211:Capsaicin; C012843:cinnamaldehyde