Generation of human CEACAM1 transgenic mice and binding of Neisseria Opa protein to their neutrophils.
BACKGROUND
Human CEACAM1 is a cell-cell adhesion molecule with multiple functions including insulin clearance in the liver, vasculogenesis in endothelial cells, lumen formation in the mammary gland, and binding of certain human pathogens.


RESULTS
Three genomic BAC clones containing the human CEACAM1 gene were microinjected into pronuclei of fertilized FVB mouse oocytes. The embryos were implanted in the oviducts of pseudopregnant females and allowed to develop to term. DNA from newborn mice was evaluated by PCR for the presence of the human CEACAM1 gene. Feces of the PCR positive offspring screened for expression of human CEACAM1. Using this assay, one out of five PCR positive lines was positive for human CEACAM1 expression and showed stable transmission to the F1 generation with the expected transmission frequency (0.5) for heterozygotes. Liver, lung, intestine, kidney, mammary gland, and prostate were strongly positive for the dual expression of both murine and human CEACAM1 and mimic that seen in human tissue. Peripheral blood and bone marrow granulocytes stained strongly for human CEACAM1 and bound Neisseria Opa proteins similar to that in human neutrophils.


CONCLUSIONS
These transgenic animals may serve as a model for the binding of human pathogens to human CEACAM1.
Gu
Angel
A
Department of Immunology, Beckman Research Institute of the City of Hope, Duarte, California, United States of America.
Zhang
Zhifang
Z
Zhang
Nan
N
Tsark
Walter
W
Shively
John E
JE
D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000235:genetics*; D001426:Bacterial Proteins / Q000378:metabolism*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000235:genetics*; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009343:Neisseria; D009504:Neutrophils / Q000378:metabolism* / Q000382:microbiology; D011485:Protein Binding; D014018:Tissue Distribution
D015703:Antigens, CD; D001426:Bacterial Proteins; C069847:CD66 antigens; D002272:Carcinoembryonic Antigen; C486183:Ceacam1 protein, mouse; D015815:Cell Adhesion Molecules
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