Preimplantation mouse embryo selection guided by light-induced dielectrophoresis.
Selection of optimal quality embryos for in vitro fertilization (IVF) transfer is critical to successful live birth outcomes. Currently, embryos are chosen based on subjective assessment of morphologic developmental maturity. A non-invasive means to quantitatively measure an embryo's developmental maturity would reduce the variability introduced by the current standard. We present a method that exploits the scaling electrical properties of pre-transfer embryos to quantitatively discern embryo developmental maturity using light-induced dielectrophoresis (DEP). We show that an embryo's DEP response is highly correlated with its developmental stage. Uniquely, this technique allows one to select, in sequence and under blinded conditions, the most developmentally mature embryos among a mixed cohort of morphologically indistinguishable embryos cultured in optimized and sub-optimal culture media. Following assay, embryos continue to develop normally in vitro. Light-induced dielectrophoresis provides a non-invasive, quantitative, and reproducible means to select embryos for applications including IVF transfer and embryonic stem cell harvest.
Valley
Justin K
JK
Berkeley Sensor & Actuator Center, Electrical Engineering and Computer Sciences, University of California, Berkeley, California, United States of America.
Swinton
Paul
P
Boscardin
W John
WJ
Lue
Tom F
TF
Rinaudo
Paolo F
PF
Wu
Ming C
MC
Garcia
Maurice M
MM
D000818:Animals; D001755:Blastocyst; D004586:Electrophoresis; D004624:Embryo Transfer / Q000379:methods / Q000592:standards; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells; D005307:Fertilization in Vitro; D008027:Light; D051379:Mice
Fertil Steril. 2005 Aug;84(2):325-30
16084872
Hum Reprod. 2006 Jan;21(1):210-6
16172148
Phys Med Biol. 1992 Jul;37(7):1499-517
1631195
Reprod Biomed Online. 2008 Oct;17(4):502-7
18854103
Nat Photonics. 2008;2(2):86-89
19789729
Lancet. 1978 Aug 12;2(8085):366
79723
Reprod Biomed Online. 2004 Aug;9(2):237-44
15333259
Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):860-4
7846067
Fertil Steril. 1999 Oct;72(4):604-9
10521095
Nature. 2005 Jul 21;436(7049):370-2
16034413
Biomed Microdevices. 2008 Jun;10(3):337-45
18071907
Lab Chip. 2010 Jan 21;10(2):165-72
20066243
Semin Reprod Med. 2005 Nov;23(4):348-53
16317623
Biophys J. 1997 Aug;73(2):1118-29
9251828
J Embryol Exp Morphol. 1970 Nov;24(3):535-53
4992748
Anal Chem. 1999 Mar 1;71(5):911-8
10079757
Lancet. 1999 Nov 6;354(9190):1579-85
10560671
Reprod Biomed Online. 2008 Oct;17(4):470-85
18854100
Lab Chip. 2009 May 21;9(10):1349-56
19417900
J Physiol. 1981 Jul;316:191-202
7320864
Anal Chem. 1998 May 1;70(9):1909-15
9599586
Nature. 1987 Dec 24-31;330(6150):769-71
3320757
Reproduction. 2007 May;133(5):931-46
17616723
Int J Dev Biol. 1998;42(7):879-84
9853817
Hum Reprod. 2009 Feb;24(2):360-6
19010807