Expressed sequence tag analysis for identification and characterization of genes related to Tributyltin (TBT) exposure in the abalone Haliotis diversicolor supertexta.
The analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and the development of resources for functional genomics. To analyze the transcriptome response to tributyltin of small abalone Haliotis diversicolor supertexta, a normalized cDNA library of hepatopancreas after exposure of animals to tributyltin was constructed. Three thousand and forty eight high quality ESTs were generated. After processing, a total of 2473 unigenes comprising 370 contigs and 2103 singlets were acquired. BLAST identified 1108 clones (45%) as known genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Simple Sequence Repeat (SSR) identification were also carried out to acquire more information on pathway and EST-SSR. According to GO categories, the total ESTs distribute across the various functional categories. In addition, 43 potential toxicology-related clusters were identified. This work provides the first set of genetic data for small abalone which is of great value for further exploitation of this species in functional genomics and toxicogenomics. Our interesting results will be mainly useful to choose biomarkers for ecotoxicological studies.
Jia
Xiwei
X
The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China; State Key Laboratory of Marine Environmental Science, Xiamen University, Environmental Science Research Center, Xiamen University, Xiamen, Fujian, 361005, China.
Zhang
Ziping
Z
The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China.
Wang
Guodong
G
The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China.
Zou
Zhihua
Z
The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China.
Wang
Shuhong
S
The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China.
Huang
Bangqin
B
State Key Laboratory of Marine Environmental Science, Xiamen University, Environmental Science Research Center, Xiamen University, Xiamen, Fujian, 361005, China.
Wang
Yilei
Y
The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China. Electronic address: ylwang@jmu.edu.cn.
D000818:Animals; D004781:Environmental Exposure / Q000032:analysis; D020224:Expressed Sequence Tags; D049851:Gastropoda / Q000187:drug effects* / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D015723:Gene Library; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin