title: A significant proportion of F2-isoprostanes in human urine are excreted as glucuronide conjugates.
abstract: The measurement of urinary F(2)-isoprostanes is noninvasive and widely used to assess in vivo oxidative stress in humans. Most studies measure urinary F(2)-isoprostanes in the free form; however, many eicosanoids are excreted as urine glucuronide conjugates. Using gas chromatography-mass spectrometry, we examined the extent of glucuronide conjugation of F(2)-isoprostanes in urine collected from healthy men (n = 20) and women (n = 15). Incubation of urine with exogenous glucuronidase led to F(2)-isoprostane concentrations that were approximately 40% higher than untreated samples (P < 0.001). We conclude that a significant proportion of F(2)-isoprostanes in urine are conjugated as glucuronides.
authors.affiliation: Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yan
authors.forename: Emilie
authors.initials: E
authors.lastname: Mas
authors.forename: Trevor A
authors.initials: TA
authors.lastname: Mori
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Croft
authors.forename: Anne E
authors.initials: AE
authors.lastname: Barden
mesh_terms: D028441:F2-Isoprostanes / Q000378:metabolism* / Q000652:urine*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D020719:Glucuronides / Q000378:metabolism* / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D028441:F2-Isoprostanes; D020719:Glucuronides