PCR detection of thermophilic spore-forming bacteria involved in canned food spoilage.
Thermophilic bacteria that form highly heat-resistant spores constitute an important group of spoilage bacteria of low-acid canned food. A PCR assay was developed in order to rapidly trace these bacteria. Three PCR primer pairs were designed from rRNA gene sequences. These primers were evaluated for the specificity and the sensitivity of detection. Two primer pairs allowed detection at the species level of Geobacillus stearothermophilus and Moorella thermoacetica/thermoautrophica. The other pair allowed group-specific detection of anaerobic thermophilic bacteria of the genera Thermoanaerobacterium, Thermoanaerobacter, Caldanerobium and Caldanaerobacter. After a single enrichment step, these PCR assays allowed the detection of 28 thermophiles from 34 cans of spoiled low-acid food. In addition, 13 ingredients were screened for the presence of these bacteria. This PCR assay serves as a detection method for strains able to spoil low-acid canned food treated at 55°C. It will lead to better reactivity in the canning industry. Raw materials and ingredients might be qualified not only for quantitative spore contamination, but also for qualitative contamination by highly heat-resistant spores.
Prevost
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CTCPA Centre Technique de la Conservation des Produits Agricoles, Site Agroparc, ZA de l'aéroport, BP 21 203, 84911, AVIGNON cedex 9, France.
Andre
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Remize
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D001431:Bacteriological Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005519:Food Preservation / Q000379:methods; D057141:Food, Preserved / Q000382:microbiology*; D020459:Genes, rRNA; D016936:Gram-Positive Endospore-Forming Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006358:Hot Temperature; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal
Appl Environ Microbiol. 1997 Aug;63(8):3139-43
9251201
Int J Food Microbiol. 2007 Nov 30;120(1-2):85-94
17644202
J Microbiol Methods. 2005 Feb;60(2):155-67
15590090
Int J Food Microbiol. 2009 Sep 30;135(1):53-9
19651455
J Appl Microbiol. 2005;99(5):1246-55
16238756
Int J Food Microbiol. 2004 Nov 15;96(3):263-72
15454316
Appl Environ Microbiol. 2000 Dec;66(12):5509-13
11097939
Int J Food Microbiol. 2008 Mar 20;122(3):229-42
18164775
PDA J Pharm Sci Technol. 2000 Sep-Oct;54(5):398-412
11057096
Int J Food Microbiol. 2009 Aug 31;134(1-2):9-15
19285357
Appl Environ Microbiol. 1987 Feb;53(2):365-70
3566270
J Bacteriol. 1991 Jan;173(2):697-703
1987160
Int J Food Microbiol. 2000 Apr 10;55(1-3):175-9
10791740
Appl Environ Microbiol. 2002 Feb;68(2):1005-9
11823254
Food Microbiol. 2006 May;23(3):220-30
16943008
Arch Microbiol. 2000 Nov;174(5):334-9
11131023
J Basic Microbiol. 2006;46(5):358-64
17009291
Syst Appl Microbiol. 2006 Nov;29(7):581-8
16458469
J Appl Microbiol. 2002;92(5):983-91
11972705