title: Variants of contextual fear conditioning are differentially impaired in the juvenile rat by binge ethanol exposure on postnatal days 4-9.
abstract: Neonatal ethanol exposure in the rat is known to partially damage the hippocampus, but such exposure causes only modest or inconsistent deficits on hippocampus-dependent behavioral tasks. This may reflect variable sensitivity of these tasks or residual function following partial hippocampal injury. The context preexposure facilitation effect (CPFE) is a variant of context conditioning in which context exposure and immediate shock occur on successive occasions. During testing, preexposed rats freeze more than non-preexposed controls. The CPFE is more sensitive to anterograde hippocampal injury than standard contextual fear conditioning (e.g., Rudy JW, O'Reilly RC. Conjunctive representations, the hippocampus, and contextual fear conditioning. Cogn Affect Behav Neurosci 2001;1:66-82). We report that rats exposed to a high binge dose of ethanol (5.25g/kg/day) over postnatal days [PD] 4-9 failed to demonstrate the CPFE when preexposed to the conditioning context on PD31, relative to sham-intubated and undisturbed controls (Experiment 1). Neonatal alcohol disrupted conditioned freezing to a much lesser extent relative to controls when context preexposure was followed by a standard context conditioning trial rather than immediate shock (Experiment 2). Fear conditioning to a discrete auditory cue (tone) was unaffected by neonatal alcohol exposure ruling out possible performance effects (Experiment 3). These findings suggest that the CPFE is an especially sensitive task for detecting hippocampal injury produced by neonatal alcohol. Mixed results with other tasks may reflect residual hippocampal function and/or the use of alternate neurobehavioral systems or "strategies" following alcohol-induced brain damage.
authors.affiliation: Department of Psychology, University of Delaware, Newark, DE, USA.
authors.forename: Nathen J
authors.initials: NJ
authors.lastname: Murawski
authors.forename: Mark E
authors.initials: ME
authors.lastname: Stanton
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D000831:Animals, Newborn; D001307:Auditory Perception / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000008:administration & dosage / Q000494:pharmacology*; D003214:Conditioning, Classical / Q000187:drug effects*; D003463:Cues; D004597:Electroshock; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005239:Fear / Q000187:drug effects*; D005260:Female; D046352:Freezing Reaction, Cataleptic / Q000187:drug effects; D008297:Male; D009483:Neuropsychological Tests; D011897:Random Allocation; D051381:Rats; D020318:Rats, Long-Evans; D013997:Time Factors
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol
references.citation: Alcohol. 2006 Feb;38(2):99-110
references.pmid: 16839856
references.citation: Neurotoxicol Teratol. 2003 Sep-Oct;25(5):519-28
references.pmid: 12972065
references.citation: Neurobiol Learn Mem. 2001 Sep;76(2):151-82
references.pmid: 11502147
references.citation: Brain Res. 2009 Jan 12;1248:162-75
references.pmid: 19007754
references.citation: Hippocampus. 2006;16(3):305-11
references.pmid: 16425237
references.citation: Behav Brain Res. 1999 Jan;98(1):77-87
references.pmid: 10210524
references.citation: Annu Rev Neurosci. 2000;23:155-84
references.pmid: 10845062
references.citation: Neurobiol Learn Mem. 2008 Jan;89(1):61-9
references.pmid: 17931914
references.citation: Hippocampus. 2004;14(1):124-34
references.pmid: 15058490
references.citation: Behav Neurosci. 2002 Aug;116(4):530-8
references.pmid: 12148921
references.citation: Learn Mem. 2008 Dec 30;16(1):38-45
references.pmid: 19117915
references.citation: Alcohol Res Health. 2001;25(3):168-74
references.pmid: 11810954
references.citation: J Neurosci. 2004 Mar 10;24(10):2431-9
references.pmid: 15014118
references.citation: Alcohol Clin Exp Res. 2000 Mar;24(3):300-6
references.pmid: 10776666
references.citation: Alcohol Clin Exp Res. 2008 Feb;32(2):277-93
references.pmid: 18162069
references.citation: Physiol Behav. 1995 Nov;58(5):1013-20
references.pmid: 8577871
references.citation: Hippocampus. 2009 Dec;19(12):1153-8
references.pmid: 19650121
references.citation: Behav Neurosci. 2006 Apr;120(2):482-7
references.pmid: 16719711
references.citation: Behav Neurosci. 1992 Apr;106(2):274-85
references.pmid: 1590953
references.citation: Exp Biol Med (Maywood). 2005 Jun;230(6):366-75
references.pmid: 15956766
references.citation: Alcohol Res Health. 2001;25(3):185-91
references.pmid: 11810956
references.citation: J Neurosci. 2006 Feb 1;26(5):1616-23
references.pmid: 16452685
references.citation: Hippocampus. 2001;11(1):8-17
references.pmid: 11261775
references.citation: J Biol Chem. 2008 Jan 4;283(1):582-592
references.pmid: 17981809
references.citation: Nat Neurosci. 1999 Dec;2(12):1120-4
references.pmid: 10570490
references.citation: Behav Brain Res. 2002 Aug 21;134(1-2):299-306
references.pmid: 12191817
references.citation: J Int Neuropsychol Soc. 2008 Nov;14(6):1022-33
references.pmid: 18954482
references.citation: Hippocampus. 2006;16(2):103-13
references.pmid: 16261555
references.citation: Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):8406-11
references.pmid: 12034877
references.citation: Neurosci Biobehav Rev. 2007;31(2):230-8
references.pmid: 16962173
references.citation: Behav Neurosci. 2008 Dec;122(6):1264-73
references.pmid: 19045946
references.citation: Int J Dev Neurosci. 2004 Aug-Oct;22(5-6):363-77
references.pmid: 15380836
references.citation: Science. 1981 Nov 20;214(4523):936-8
references.pmid: 6795717
references.citation: Nature. 1982 Jun 24;297(5868):681-3
references.pmid: 7088155
references.citation: Behav Brain Res. 1997 Nov;88(2):261-74
references.pmid: 9404635
references.citation: Science. 1992 May 1;256(5057):675-7
references.pmid: 1585183
references.citation: Alcohol Clin Exp Res. 1993 Jun;17(3):610-22
references.pmid: 8333592
references.citation: Behav Neurosci. 2009 Oct;123(5):1148-52
references.pmid: 19824781
references.citation: Learn Mem. 2009 Sep 30;16(10):573-85
references.pmid: 19794181
references.citation: Neurotoxicol Teratol. 1997 Nov-Dec;19(6):435-46
references.pmid: 9392779
references.citation: Behav Neurosci. 2005 Feb;119(1):154-63
references.pmid: 15727521
references.citation: Alcohol Clin Exp Res. 1990 Feb;14(1):107-18
references.pmid: 1689970
references.citation: Early Hum Dev. 1979 Mar;3(1):79-83
references.pmid: 118862
references.citation: Alcohol Res Health. 2001;25(3):159-67
references.pmid: 11810953
references.citation: Alcohol Clin Exp Res. 2002 Jan;26(1):83-93
references.pmid: 11821658
references.citation: JAMA. 2003 Dec 10;290(22):2996-9
references.pmid: 14665662
references.citation: Cerebellum. 2004;3(3):178-87
references.pmid: 15543808
references.citation: Philos Trans R Soc Lond B Biol Sci. 1971 Jul 1;262(841):23-81
references.pmid: 4399412
references.citation: Mich Med. 1988 May;87(5):262-4, 268
references.pmid: 3380026
references.citation: Brain Res Brain Res Rev. 2001 Aug;36(1):60-90
references.pmid: 11516773
references.citation: Alcohol Clin Exp Res. 2003 Dec;27(12):2009-16
references.pmid: 14691390
references.citation: Exp Biol Med (Maywood). 2005 Jun;230(6):357-65
references.pmid: 15956765
references.citation: Neurosci Biobehav Rev. 2004 Nov;28(7):675-85
references.pmid: 15555677
references.citation: Teratology. 1991 Aug;44(2):147-63
references.pmid: 1925974
references.citation: Eur J Neurosci. 2007 Mar;25(5):1278-86
references.pmid: 17355254
references.citation: Behav Neurosci. 1993 Dec;107(6):1093-8
references.pmid: 8136063
references.citation: Alcohol Clin Exp Res. 2007 Dec;31(12):2073-82
references.pmid: 17949464
references.citation: Dev Psychobiol. 1996 Jul;29(5):433-52
references.pmid: 8809494
references.citation: Neurobiol Learn Mem. 1995 Nov;64(3):265-75
references.pmid: 8564380
references.citation: Lancet. 1986 Nov 22;2(8517):1222
references.pmid: 2877359
references.citation: Dev Psychobiol. 2007 Apr;49(3):243-57
references.pmid: 17380527
references.citation: Behav Neurosci. 1999 Dec;113(6):1189-203
references.pmid: 10636298
references.citation: Hippocampus. 2002;12(2):186-205
references.pmid: 12000118
references.citation: Behav Brain Res. 2010 Oct 15;212(2):133-42
references.pmid: 20385174
references.citation: Hippocampus. 2005;15(6):808-14
references.pmid: 16010664
references.citation: Synapse. 2008 Aug;62(8):566-73
references.pmid: 18512209
references.citation: J Neurosci. 2006 May 17;26(20):5484-91
references.pmid: 16707800
references.citation: Hippocampus. 2008;18(7):710-8
references.pmid: 18446823
references.citation: Neurosci Res. 2005 Apr;51(4):417-25
references.pmid: 15740804
references.citation: Alcohol. 1999 Feb;17(2):125-31
references.pmid: 10064380
references.citation: Dev Med Child Neurol. 1999 Oct;41(10):652-9
references.pmid: 10587040
references.citation: Alcohol Clin Exp Res. 2001 May;25(5):734-45
references.pmid: 11371723
references.citation: Neurobiol Learn Mem. 2011 Feb;95(2):190-8
references.pmid: 21129493
references.citation: Alcohol Clin Exp Res. 1998 Apr;22(2):279-94
references.pmid: 9581631
references.citation: Neurosci Biobehav Rev. 2007;31(2):239-45
references.pmid: 17097730
references.citation: Behav Neurosci. 1998 Aug;112(4):863-74
references.pmid: 9733192
references.citation: Hippocampus. 1997;7(2):232-8
references.pmid: 9136052
references.citation: Behav Neurosci. 2007 Aug;121(4):721-31
references.pmid: 17663597
references.citation: Behav Neurosci. 2006 Apr;120(2):229-40
references.pmid: 16719687
references.citation: Cogn Affect Behav Neurosci. 2001 Mar;1(1):66-82
references.pmid: 12467104
references.citation: Neurotoxicol Teratol. 2003 Jul-Aug;25(4):447-58
references.pmid: 12798962