title: Self-compatibility in 'Cristobalina' sweet cherry is not associated with duplications or modified transcription levels of S-locus genes.
abstract: Sweet cherry shows S-RNase-based gametophytic self-incompatibility, which prevents self- and cross-fertilization between genetically related individuals. The specificity of the self-incompatible reaction is determined by two genes located in the S-locus. These encode a pistil-expressed ribonuclease (S-RNase) that inhibits self pollen tube growth, and a pollen-expressed F-box protein (SFB) that may be involved in the cytotoxicity of self-S-RNases. Initial genetic and pollination studies in a self-compatible sweet cherry cultivar, 'Cristobalina' (S (3) S (6)), showed that self-compatibility was caused by the loss of pollen function of both haplotypes (S (3) and S (6)). In this study, we further characterize self-compatibility in this genotype by molecular analysis of the S-locus. DNA blot analyses using S-RNase and SFB probes show no duplications of 'Cristobalina' S-locus genes or differences in the restriction patterns when compared with self-incompatible cultivars with the same S-genotype. Furthermore, reverse transcriptase-PCR of S-locus genes and quantitative reverse transcription-PCR of SFBs revealed no differences at the transcription level when compared with a self-incompatible genotype. The results of this study show that no differences at the S-locus can be correlated with self-compatibility, indicating the possible involvement of non-S-locus modifiers in self-incompatibility breakdown in this cultivar.
authors.affiliation: Centro de Investigación y Tecnología Agroalimentaria de Aragón, Zaragoza, Spain. awunsch@aragon.es
authors.forename: A
authors.initials: A
authors.lastname: Wünsch
authors.forename: R
authors.initials: R
authors.lastname: Tao
authors.forename: J I
authors.initials: JI
authors.lastname: Hormaza
mesh_terms: D044783:F-Box Proteins / Q000235:genetics*; D035264:Flowers / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D020440:Gene Duplication / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005838:Genotype; D011058:Pollen / Q000235:genetics; D027861:Prunus / Q000201:enzymology / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012260:Ribonucleases / Q000235:genetics*; D057945:Self-Fertilization; D014158:Transcription, Genetic / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D044783:F-Box Proteins; D018749:RNA, Plant; D012260:Ribonucleases
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