Adenosine triphosphate concentration of exhaled breath condensate in asthma.
BACKGROUND
Purinergic signaling is involved in asthma pathogenesis. Not only adenosine but also adenosine triphosphate (ATP) might play a role, but human evidence is scarce. ATP can be measured in exhaled breath condensate (EBC), a noninvasive airway sample suggested as being suitable for patient monitoring. We determined EBC ATP concentration in asthma, investigated its relation to disease parameters, and calculated airway ATP level.


METHODS
EBC was collected from 45 patients with persistent asthma (age 34.7 +/- 13.2 years; FEV(1), 87.0 +/- 15.5% predicted; mean +/- SD) and 32 healthy control subjects (age 36.9 +/- 12.6 years; FEV(1), 98.9 +/- 9.9% predicted). Exhaled nitric oxide concentration (FeNO) and lung function were measured, and Asthma Control Test (ACT) score was obtained. EBC ATP was measured in luciferin-luciferase assay. Airway ATP concentration was calculated using dilution estimated from conductivity of vacuum-treated EBC samples. Parametric tests were applied in the analyses. ATP concentrations and nitric oxide levels were logarithmically transformed.


RESULTS
EBC ATP and calculated airway ATP concentrations were not elevated in asthma, and none of them was related to FeNO or ACT score. EBC ATP concentration was influenced by airway droplet dilution (r = -0.32, P < .05), and there was a relation between calculated airway ATP level and FEV(1) (r = -0.35, P < .05).


CONCLUSIONS
EBC ATP concentration does not seem to be useful for asthma monitoring. The relation between EBC mediator concentration and EBC conductivity highlights the importance of further standardization of EBC methodology and the need for more studies to understand airway droplet formation.
Lázár
Zsófia
Z
Department of Pulmonology, Hungarian Academy of Sciences, Semmelweis University, Budapest, Hungary.
Cervenak
László
L
Orosz
Márta
M
Gálffy
Gabriella
G
Komlósi
Zsolt I
ZI
Bikov
András
A
Losonczy
György
G
Horváth
Ildikó
I
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000328:Adult; D000403:Airway Resistance; D001249:Asthma / Q000175:diagnosis / Q000378:metabolism* / Q000503:physiopathology; D015415:Biomarkers / Q000378:metabolism; D001944:Breath Tests; D001992:Bronchoalveolar Lavage Fluid; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D045853:Exhalation; D015633:Extravascular Lung Water / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D015415:Biomarkers; D000255:Adenosine Triphosphate