[The influence of pH on cholinesterase hydrolysis of alpha-naphthylacetate in the presence of some cationic detergents].
The influence of some cationic detergents on the catalytic activity of the horse blood plasma cholinesterase in reaction of hydrolysis of alpha-naphthylacetate at different pH were investigated. It was shown, that in the absence of detergents in acid pH of the reaction medium the Km value increases, but V remain constant. In the range of pH from 8.5 to 5.0 in the presence of detergents the Km and V values are not practically changed. That is why the activation of cholinesterase hydrolysis of alpha-naphthylacetate in the presence of detergents is considerably higher than that of the neutral pH.
Kuznetsova
L P
LP
Nikitina
E R
ER
Sochilina
E E
EE
Vasil'eva
K A
KA
D000818:Animals; D002091:Butyrylcholinesterase / Q000097:blood / Q000737:chemistry*; D002412:Cations; D002593:Cetrimonium Compounds / Q000737:chemistry*; D002594:Cetylpyridinium / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry*; D006736:Horses; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D009284:Naphthols / Q000737:chemistry*
D002412:Cations; D002593:Cetrimonium Compounds; D003902:Detergents; D009284:Naphthols; C027876:alpha-naphthyl acetate; D002594:Cetylpyridinium; D002091:Butyrylcholinesterase