title: T-cell receptor early signalling complex activation in response to interferon-alpha receptor stimulation.
abstract: Signalling through the IFNalphaR (interferon-alpha receptor) and TCR (T-cell receptor) in Jurkat T lymphocytes results in distinct immune responses. Despite this both receptors elicit ERK (extracellular-signal-regulated kinase)/MAPK (mitogen-activated protein kinase) phosphorylation. Vav and Slp76 are shown to be required for IFNalpha (interferon-alpha)-stimulated ERK activity. These form a subset of proteins which behave identically on stimulation of both receptors. TCR deletion abrogates IFNalphaR-stimulated MAPK activity, whereas the canonical JAK/STAT (Janus kinase/signal transducer and activator of transcription) pathway is unaffected. Thus recruitment of the intact TCR ESC (early signalling complex) is necessary for this downstream MAPK response. Despite using a common ESC, stimulation of the IFNalphaR does not produce the transcriptional response associated with TCR. Up-regulation of the MAPK pathway by IFNalphaR might be important to ensure that the cell responds to only one stimulant.
authors.affiliation: Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E6BT, UK.
authors.forename: Claire N
authors.initials: CN
authors.lastname: Stevens
authors.forename: Ann-Marie
authors.initials: AM
authors.lastname: Simeone
authors.forename: Susan
authors.initials: S
authors.lastname: John
authors.forename: Zamal
authors.initials: Z
authors.lastname: Ahmed
authors.forename: Orso M
authors.initials: OM
authors.lastname: Lucherini
authors.forename: C Tatiana
authors.initials: CT
authors.lastname: Baldari
authors.forename: John E
authors.initials: JE
authors.lastname: Ladbury
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D053633:Receptor, Interferon alpha-beta / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D014162:Transfection
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D010750:Phosphoproteins; D011948:Receptors, Antigen, T-Cell; C092947:SLP-76 signal Transducing adaptor proteins; D053633:Receptor, Interferon alpha-beta; D020928:Mitogen-Activated Protein Kinases
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