Characterisation of gp34, a GPI-anchored protein expressed by schizonts of Theileria parva and T. annulata.
Using bioinformatics tools, we searched the predicted Theileria annulata and T. parva proteomes for putative schizont surface proteins. This led to the identification of gp34, a GPI-anchored protein that is stage-specifically expressed by schizonts of both Theileria species and is downregulated upon induction of merogony. Transfection experiments in HeLa cells showed that the gp34 signal peptide and GPI anchor signal are also functional in higher eukaryotes. Epitope-tagged Tp-gp34, but not Ta-gp34, expressed in the cytosol of COS-7 cells was found to localise to the central spindle and midbody. Overexpression of Tp-gp34 and Ta-gp34 induced cytokinetic defects and resulted in accumulation of binucleated cells. These findings suggest that gp34 could contribute to important parasite-host interactions during host cell division.
Xue
Gondga
G
Division of Molecular Pathobiology, DCR-VPH, Vetsuisse Faculty, University of Bern, CH-3013 Bern, Switzerland.
von Schubert
Conrad
C
Hermann
Pascal
P
Peyer
Martina
M
Maushagen
Regina
R
Schmuckli-Maurer
Jacqueline
J
Bütikofer
Peter
P
Langsley
Gordon
G
Dobbelaere
Dirk A E
DA
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D019295:Computational Biology / Q000379:methods; D048749:Cytokinesis; D015870:Gene Expression; D006367:HeLa Cells; D006790:Host-Parasite Interactions; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification*; D052939:Schizonts / Q000378:metabolism*; D016415:Sequence Alignment; D016796:Theileria annulata / Q000235:genetics / Q000378:metabolism*; D016797:Theileria parva / Q000235:genetics / Q000378:metabolism*
D008565:Membrane Proteins; D021382:Protein Sorting Signals; D015800:Protozoan Proteins
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