title: Dendrigraft poly-L-lysine: a non-immunogenic synthetic carrier for antibody production.
abstract: An easily synthesized DendriGraft poly-lysine DGL-G3 (third generation) was shown to act as an efficient carrier for raising antibodies directed against small molecules. The immunological properties of three different forms of DGL-G3 were investigated: the native form (molecular weight 22 kDa bearing a mean number of 123 surface amino groups as TFA salts), a form modified at the C-terminus by fluorescein (fluorescein-DGL-G3), and last a surface-modified form bearing histamine (DGL-G3-Histamine). Our studies demonstrate the native DGL-G3 to be inefficient in eliciting antibody production in rabbits. Immunizations of rabbits using the core-modified fluorescein-DGL-G3 or the surface-modified DGL-G3-histamine conjugate failed in eliciting antibody production. Conversely, following a primary immunization using a BSA-histamine conjugate, a second immunization with DGL-G3-histamine conjugate improved the production of specific hapten-directed antibodies, which demonstrates the utility of DGL-G3 as a carrier for the production of highly specific antibody against haptens.
authors.affiliation: IFREMER, Université Montpellier 2, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5119, Ecosystèmes Lagunaires, place E. Bataillon, CC80, 34095 Montpellier cedex 5, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Romestand
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Rolland
authors.forename: Auguste
authors.initials: A
authors.lastname: Commeyras
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Coussot
authors.forename: Isabelle
authors.initials: I
authors.lastname: Desvignes
authors.forename: Robert
authors.initials: R
authors.lastname: Pascal
authors.forename: Odile
authors.initials: O
authors.lastname: Vandenabeele-Trambouze
mesh_terms: D000818:Animals; D000917:Antibody Formation; D004337:Drug Carriers; D005260:Female; D011107:Polylysine / Q000008:administration & dosage*; D011817:Rabbits
chemical_list: D004337:Drug Carriers; D011107:Polylysine