[Enzyme immunoassay for determination of sulfamethoxypyridazine in honey].
An enzyme immunoassay technique for the detection of sulfamethoxypyridazine in honey, developed using rabbit polyclonal antibodies raised against N-sulfonyl-4-aminobutyric acid, which contains a structural group characteristic of sulfonamides, is proposed. Under the optimized conditions, the sulfamethoxypyridazine detection limit was 0.05 ng/ml, with the entire analysis procedure taking 2 h. In total, 24 honey samples were tested using the protocol based on tenfold dilutions of samples without their preliminary treatment.
Tafintseva
I Iu
IIu
Zherdev
A V
AV
Eremin
S A
SA
Dzantiev
B B
BB
D000818:Animals; D000906:Antibodies; D006722:Honey / Q000032:analysis*; D007124:Immunoenzyme Techniques; D011817:Rabbits; D012680:Sensitivity and Specificity; D013421:Sulfamethoxypyridazine / Q000032:analysis*
D000906:Antibodies; D013421:Sulfamethoxypyridazine