title: Decreased food intake following overfeeding involves leptin-dependent and leptin-independent mechanisms.
abstract: After a period of forced overfeeding, many individuals actively compensate for this weight gain by reducing food intake and maintaining this state of hypophagia well into the post-overfeeding period. Our central goal is to define the mechanism underlying this adaptive reduction in food intake. When male Long Evans rats were implanted with indwelling gastric cannula and overfed a liquid low-fat (10% fat) diet for 17 days, overfed rats exhibited increased weight gain (P<0.01) but decreased food intake, and this hypophagia persisted for 4-6 days post-overfeeding (P<0.05). Leptin levels were increased 8-fold by overfeeding (P<0.01), yet returned to baseline within 2 days post-overfeeding, despite the persistent hypophagia. Energy expenditure and oxygen consumption (VO2) were increased on the first day post-overfeeding (P<0.05), but subsequently normalized prior to the normalization of food intake. Lastly, in leptin receptor deficient Obese Zucker (fa/fa) rats, overfeeding produced a significant decrease in food intake during active overfeeding. However, food intake returned to near baseline levels within one day post-overfeeding. Contrastingly, food intake remained suppressed in lean controls for 6 days post-overfeeding. Thus intact leptin signaling is not required for the decrease in food intake that occurs during overfeeding, but the ability to maintain this hypophagia is substantially impaired in the absence of leptin signaling. In addition, this post-overfeeding leptin effect appears to occur despite the fact that leptin levels normalize relatively rapidly post-overfeeding.
authors.affiliation: Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA.
authors.forename: Christy L
authors.initials: CL
authors.lastname: White
authors.forename: Megan N
authors.initials: MN
authors.lastname: Purpera
authors.forename: Kenny
authors.initials: K
authors.lastname: Ballard
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Morrison
mesh_terms: D000222:Adaptation, Physiological; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004435:Eating / Q000502:physiology*; D004734:Energy Metabolism; D005223:Fats / Q000378:metabolism; D015870:Gene Expression; D006728:Hormones / Q000097:blood; D020738:Leptin / Q000097:blood / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D051381:Rats; D020318:Rats, Long-Evans; D011924:Rats, Zucker; D015430:Weight Gain
chemical_list: D005223:Fats; D006728:Hormones; D020738:Leptin
references.citation: Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):18276-9
references.pmid: 17986612
references.citation: J Endocrinol Invest. 2006 Nov;29(10):863-8
references.pmid: 17185893
references.citation: Int J Obes (Lond). 2006 Jul;30(7):1160-2
references.pmid: 16477273
references.citation: Physiol Behav. 2007 Jan 30;90(1):82-94
references.pmid: 17028044
references.citation: J Anim Sci. 2002 Apr;80(4):1083-9
references.pmid: 12002315
references.citation: Physiol Behav. 1984 Dec;33(6):863-9
references.pmid: 6537515
references.citation: Am J Physiol. 1989 Mar;256(3 Pt 2):R766-71
references.pmid: 2646957
references.citation: Am J Physiol Regul Integr Comp Physiol. 2000 Apr;278(4):R797-805
references.pmid: 10749765
references.citation: Nat Genet. 1996 May;13(1):18-9
references.pmid: 8673096
references.citation: Am J Physiol. 1992 Jun;262(6 Pt 2):R1025-32
references.pmid: 1621856
references.citation: Am J Physiol. 1996 Sep;271(3 Pt 2):R819-23
references.pmid: 8853408
references.citation: Biochem Biophys Res Commun. 1996 Jul 16;224(2):597-604
references.pmid: 8702432
references.citation: Science. 2007 Sep 7;317(5843):1355
references.pmid: 17690262
references.citation: Am J Physiol. 1997 Aug;273(2 Pt 2):R725-30
references.pmid: 9277561
references.citation: Cell Metab. 2007 Jul;6(1):5-12
references.pmid: 17618852
references.citation: J Clin Invest. 1996 May 1;97(9):2020-6
references.pmid: 8621790
references.citation: Am J Physiol. 1999 May;276(5):R1545-9
references.pmid: 10233050
references.citation: Front Horm Res. 2000;26:42-56
references.pmid: 10767940
references.citation: J Clin Invest. 2005 Mar;115(3):703-10
references.pmid: 15711637
references.citation: J Neurosci. 1999 Mar 15;19(6):2362-7
references.pmid: 10066286
references.citation: Int J Obes Relat Metab Disord. 1999 Nov;23(11):1105-17
references.pmid: 10578199
references.citation: Proc Nutr Soc. 2000 Aug;59(3):359-71
references.pmid: 10997652
references.citation: Science. 2004 Apr 2;304(5667):108-10
references.pmid: 15064420
references.citation: Proc Soc Exp Biol Med. 1989 May;191(1):82-9
references.pmid: 2654948
references.citation: J Clin Endocrinol Metab. 2005 Jan;90(1):359-65
references.pmid: 15483088
references.citation: Metabolism. 1998 Apr;47(4):429-34
references.pmid: 9550541
references.citation: Physiol Behav. 2008 May 23;94(2):178-86
references.pmid: 18281067
references.citation: Appetite. 1986 Dec;7(4):381-6
references.pmid: 3539015
references.citation: J Clin Endocrinol Metab. 2004 Feb;89(2):936-9
references.pmid: 14764817
references.citation: Endocrinology. 2006 Jan;147(1):3-8
references.pmid: 16166213
references.citation: Physiol Behav. 2005 Dec 15;86(5):614-22
references.pmid: 16225895
references.citation: J Nutr. 1986 Dec;116(12):2536-46
references.pmid: 3543262
references.citation: Endocrinology. 2002 Oct;143(10):3779-84
references.pmid: 12239088
references.citation: J Physiol. 2007 Sep 1;583(Pt 2):425-30
references.pmid: 17584845
references.citation: Diabetes. 2006 Dec;55(12):3387-93
references.pmid: 17130484
references.citation: Hepatology. 2005 Oct;42(4):905-14
references.pmid: 16175602
references.citation: Front Horm Res. 2008;36:61-72
references.pmid: 18230894
references.citation: FEBS Lett. 1995 Jul 24;368(3):488-90
references.pmid: 7635205
references.citation: Prog Food Nutr Sci. 1988;12(1):45-72
references.pmid: 3041450
references.citation: Clin Genet. 2006 Oct;70(4):295-301
references.pmid: 16965320
references.citation: J Nutr. 1970 Sep;100(9):1041-8
references.pmid: 5456549
references.citation: Physiol Behav. 2007 Aug 15;91(5):473-8
references.pmid: 17137609
references.citation: Nutr Metab (Lond). 2006 Jul 12;3:25
references.pmid: 16836744
references.citation: Metabolism. 1996 Aug;45(8):1042-50
references.pmid: 8769366