7-aa peptide mimic from HVR1 of HCV protects hepatic injury in rats by reduced expression of key pro-inflammatory factors.
Hepatitis C virus (HCV) infection related hepatitis remains one of the most serious health problems world-wide. During investigation of hypervariable region 1 (HVR1) of HCV, a seven amino acids mimic peptide (GQTYTSG, named 7-P) was identified within the N-terminal of HVR1 that could protect porcine serum (PS)-induced hepatic injury in rats. 7-P could not only decrease the serum levels of aminotransferases (ALT, AST), alkaline phosphatase (AKP), and billirubin, but were also effective in reversing histopathological evidence of PS-induced rat chronic liver injury. The protective mechanism was investigated in detail using Affymetrix microarrays analysis. The bioinformatic data was confirmed by western blotting. Further studies revealed that IL-1beta, tumor necrosis factor receptor superfamily 14 (TNFRSF14), intercellular adhesion molecule 2 (ICAM2), sterol regulatory element-binding protein 1 (SREBP 1), Prohibitin (PHB), and inhibitor of DNA binding 2 (Id2) were the key factors for 7-P-induced hepatoprotective effect. Together these data suggest that 7-P mimic from HVR1 of HCV protects hepatic injury by reducing the expression of key pro-inflammatory factors. 7-P might have potential therapeutic application for hepatitis in human beings.
Zhao
Wanzhou
W
R&D Center, Nanjing, China.
Wang
Lifeng
L
Chen
Pingyan
P
Zhao
Jun
J
Qi
Yang
Y
Chi
Shuping
S
Sun
Jie
J
Zhu
Lei
L
Cheng
Yun
Y
D000818:Animals; D005260:Female; D006505:Hepatitis / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology*; D000091402:Prohibitins; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
C400562:HVR1 protein, Hepatitis C virus; D018836:Inflammation Mediators; D009842:Oligopeptides; C000717328:PHB protein, human; C000717332:Phb protein, rat; D000091402:Prohibitins; D014764:Viral Proteins