title: [Effects of storage medium and temperature on short-term storage of Acipenser baerii eggs].
abstract: This paper studied the effects of different storage medium (coelomic fluid, Hepes solution, and modified Ringer's solution), temperature (4 degrees C and 16 degrees C), and duration (4 h, 8 h, 16 h, and 24 h) on the short-term storage of Siberian sturgeon (Acipenser baerii) eggs. The results showed that storage medium, temperature, and duration all had significant effects on the fertilization rate, hatching success, and malformation of newly hatched larvae (P < 0.05). With the increase of storage duration, the fertilization and hatching rates decreased, while the malformation rate of hatched larvae increased. The A. baerii eggs stored at 16 degrees C had higher fertilization, hatching, and malformation rates but shorter holding time than those stored at 4 degrees C. The optimal conditions for the short-term storage of A. baerii eggs were Hepes solution prepared according to the biochemical composition of A. baerii coelomic fluid, 16 degrees C, and 4 h, under which, 86.36% fertilization rate, 94.74% hatching rate, and zero malformation would be obtained.
authors.affiliation: Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China. zhangtaoyfi@163.com
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Shi-Wei
authors.initials: SW
authors.lastname: Yan
authors.forename: Ping
authors.initials: P
authors.lastname: Zhuang
authors.forename: Long-Zhen
authors.initials: LZ
authors.lastname: Zhang
authors.forename: Mei-Ping
authors.initials: MP
authors.lastname: Tian
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Da-Hai
authors.initials: DH
authors.lastname: Zhang
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Yao
mesh_terms: D000818:Animals; D003470:Culture Media; D005399:Fishes / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D012800:Siberia; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods / Q000662:veterinary*
chemical_list: D003470:Culture Media