Distribution and proliferation of bone marrow cells in the brain after pilocarpine-induced status epilepticus in mice.
The distribution of bone marrow cells in brain areas during the acute period after pilocarpine-induced status epiepticus (SE) was investigated here. To achieve this, we generated chimeric mice by engrafting bone marrow cells from enhanced green fluorescent protein (eGFP) transgenic mice. GFP(+) bone marrow-derived cells were found throughout the brain, predominantly in the hippocampus. As expected, these cells exhibited the characteristics of microglia. The pattern of distribution, proliferation, and differentiation of GFP(+)cells changes as a function of intensity and time following SE. This pattern is also a consequence of the inflammatory response, which is followed by the progressive neuronal damage that is characteristic of the pilocarpine model.
Longo
Beatriz
B
Laboratório de Engenharia Tecidual e Imunofarmacologia, CPqGM/FIOCRUZ, Bahia, Brazil.
Romariz
Simone
S
Blanco
Miriam Marcela
MM
Vasconcelos
Juliana Fraga
JF
Bahia
Luciana
L
Soares
Milena Botelho Pereira
MB
Mello
Luiz E
LE
Ribeiro-dos-Santos
Ricardo
R
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D001921:Brain / Q000473:pathology / Q000601:surgery*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D049109:Cell Proliferation; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010862:Pilocarpine / Q000009:adverse effects; D013226:Status Epilepticus / Q000139:chemically induced / Q000473:pathology / Q000601:surgery*; D013997:Time Factors
D009419:Nerve Tissue Proteins; C485184:enhanced green fluorescent protein; D010862:Pilocarpine; D049452:Green Fluorescent Proteins; D001973:Bromodeoxyuridine