title: A high-performance liquid chromatography assay to monitor the new antiepileptic drug lacosamide in patients with epilepsy.
abstract: A simple high-performance liquid chromatographic micromethod is described for the quantitation of the new antiepileptic drug lacosamide in serum of patients. Serum (100 microL) was first precipitated with 10 microL 60% perchloric acid and 10 microL supernatant injected directly into the high-performance liquid chromatograph. Chromatographic separation was achieved by use of a steel cartridge column (125 x 3 mm inside diameter) packed with Hypersil BDS C-18, at 40 degrees C, and with a gradient elution system comprising methanol, formic acid and water. The eluent was monitored at 215 nm by diode array detection and the calibration curve was linear in the range of 10 to 250 micromol/L. Recovery ranged from 99% to 106%. The limit of quantification was 1 micromol/L and the intrabatch and interbatch coefficients of variation were less than 5%. No interference from commonly prescribed antiepileptic drugs (clobazam, clonazepam, carbamazepine, carbamazepine-10,11-epoxide, gabapentin, lamotrigine, levetiracetam, oxcarbazepine, phenobarbital, phenytoin, primidone, pregabalin, valproic acid, and vigabatrin) was observed, so the method can be used to routinely monitor lacosamide in patients on polytherapy antiepileptic drug regimens.
authors.affiliation: Department of Clinical and Experimental Epilepsy, Pharmacology and Therapeutics Unit, UCL-Institute of Neurology, National Hospital for Neurology and Neurosurgery, Queen Square, London, UK.
authors.forename: Clare
authors.initials: C
authors.lastname: Greenaway
authors.forename: Neville
authors.initials: N
authors.lastname: Ratnaraj
authors.forename: Josemir W
authors.initials: JW
authors.lastname: Sander
authors.forename: Philip N
authors.initials: PN
authors.lastname: Patsalos
mesh_terms: D000081:Acetamides / Q000097:blood* / Q000627:therapeutic use*; D000927:Anticonvulsants / Q000097:blood* / Q000627:therapeutic use*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D016903:Drug Monitoring / Q000379:methods; D004827:Epilepsy / Q000097:blood* / Q000188:drug therapy*; D006801:Humans; D000078334:Lacosamide; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D000081:Acetamides; D000927:Anticonvulsants; D000078334:Lacosamide