Antimicrobial activity of a halocidin-derived peptide resistant to attacks by proteases.
Cationic antimicrobial peptides (AMPs) have attracted a great deal of interest as a promising candidate for a novel class of antibiotics that might effectively treat recalcitrant infections caused by a variety of microbes that are resistant to currently available drugs. However, the AMPs are inherently limited in that they are inevitably susceptible to attacks by proteases generated by human and pathogenic microbes; this vulnerability severely hinders their pharmaceutical use in human therapeutic protocols. In this study, we report that a halocidin-derived AMP, designated HG1, was found to be resistant to proteolytic degradation. As a result of its unique structural features, HG1 proved capable of preserving its antimicrobial activity after incubation with trypsin, chymotrypsin, and human matrix metalloprotease 7 (MMP-7). Additionally, HG1 was observed to exhibit profound antimicrobial activity in the presence of fluid from human skin wounds or proteins extracted from the culture supernatants of Staphylococcus aureus and Pseudomonas aeruginosa. Greater understanding of the structural motifs of HG1 required for its protease resistance might provide feasible ways to solve the problems intrinsic to the development of an AMP-based antibiotic.
Shin
Yong Pyo
YP
Department of Biotechnology, Hoseo University, Baebang, Sechuli, Asan City 336-795, Chungnam, South Korea.
Park
Ho Jin
HJ
Shin
Seo Hwa
SH
Lee
Young Shin
YS
Park
Seungmi
S
Jo
Sungho
S
Lee
Yong Ho
YH
Lee
In Hee
IH
D002918:Chymotrypsin / Q000378:metabolism; D006801:Humans; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D014357:Trypsin / Q000378:metabolism
D010455:Peptides; C460842:halocidin; D002918:Chymotrypsin; D014357:Trypsin; D020783:Matrix Metalloproteinase 7
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