title: Proteomic screen for potential regulators of M-phase entry and quality of meiotic resumption in Xenopus laevis oocytes.
abstract: The quality of oocytes depends largely on the capacity to resume meiotic maturation. In Xenopus laevis, only fully grown oocytes react to progesterone stimulation by resumption of meiotic maturation associated with the entry into the meiotic M-phase. Proteins involved in this process are poorly known. To identify novel proteins regulating M-phase entry, we performed a differential proteomic screen. We compared proteomes of fully grown stage VI oocytes characterized as poorly or highly responsive to progesterone treatment. The comparison of 2-D gels allowed us to identify several spots including two specifically present in highly responsive oocytes and two specifically present in poorly responsive ones. By mass spectrometry we identified the two proteins specifically present in highly responsive oocytes as inosine 5'monophosphate cyclohydrolase and YjgF homologues, and the two specifically present in poorly responsive oocytes as elongation factor 2 (EF2) and S-adenosyl-L-homocysteine hydrolase (SAHH). The proteins specifically expressed in highly responsive oocytes may participate in the stimulation of meiotic maturation and M-phase entry, while the proteins specifically present in poorly maturing oocytes may participate in the inhibition of meiotic resumption.
authors.affiliation: CNRS UMR 6061/University of Rennes 1, Institute of Genetics and Development of Rennes, Faculty of Medicine, Rennes, France.
authors.forename: Romain
authors.initials: R
authors.lastname: D'Inca
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Marteil
authors.forename: Franck
authors.initials: F
authors.lastname: Bazile
authors.forename: Aude
authors.initials: A
authors.lastname: Pascal
authors.forename: Nathalie
authors.initials: N
authors.lastname: Guitton
authors.forename: Régis
authors.initials: R
authors.lastname: Lavigne
authors.forename: Laurent
authors.initials: L
authors.lastname: Richard-Parpaillon
authors.forename: Jacek Z
authors.initials: JZ
authors.lastname: Kubiak
mesh_terms: D000818:Animals; D005260:Female; D008540:Meiosis / Q000187:drug effects / Q000502:physiology*; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D011374:Progesterone / Q000494:pharmacology; D040901:Proteomics / Q000379:methods*; D029867:Xenopus Proteins / Q000032:analysis*; D014982:Xenopus laevis
chemical_list: D029867:Xenopus Proteins; D011374:Progesterone