Preparation of Xenopus tropicalis whole chromosome painting probes using laser microdissection and reconstruction of X. laevis tetraploid karyotype by Zoo-FISH.
Laser microdissection was used for the preparation of whole chromosome painting probes in Silurana (Xenopus) tropicalis. Subsequent cross-species fluorescence in situ hybridization (Zoo-FISH) on its tetraploid relative Xenopus laevis revealed persistence of chromosomal quartets even after 50-65 million years of separate evolution. Their arrangement is in a partial concordance with previous experiments based on similarity of a high-resolution replication banding pattern. Further support for an allotetraploid origin of X. laevis was given by hybridization with a probe derived from the smallest X. tropicalis chromosome (Xt10). Here, pericentric areas of both arms of Xl 14 and 18 were stained, indicating intrachromosomal rearrangements. The positions of signals were not in agreement with the chromosomal quartets revealed by painting probes Xt 8 and 9 (Xl 11 + 14 and Xl 15 + 18, respectively). This suggests that both X. tropicalis chromosomes underwent non-reciprocal translocation of Xt10 separately in at least two different ancient ancestors. In addition, the observed translocation events could explain the origin of individuals with 18 chromosomes in diploid karyotypes, probably extinct after the genesis of the allotetraploid X. laevis (2n = 36).
Krylov
Vladimir
V
Faculty of Science, Charles University in Prague, Vinicna 7, 128 44 Prague 2, Czech Republic. vkrylov@natur.cuni.cz
Kubickova
Svatava
S
Rubes
Jiri
J
Macha
Jaroslav
J
Tlapakova
Tereza
T
Seifertova
Eva
E
Sebkova
Natasa
N
D000818:Animals; D005075:Biological Evolution; D002871:Chromosome Banding; D002875:Chromosomes / Q000235:genetics*; D020732:Cytogenetic Analysis / Q000379:methods*; D015342:DNA Probes; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D042282:Microdissection; D014178:Translocation, Genetic; D014981:Xenopus / Q000235:genetics*; D014982:Xenopus laevis / Q000235:genetics
D015342:DNA Probes
Chromosome Res. 2008;16(6):863-73
18709543
Dev Comp Immunol. 2001 Mar;25(2):149-57
11113284
BMC Genomics. 2006 Aug 29;7:219
16939647
Methods Cell Sci. 2001;23(1-3):37-55
11741143
Chromosome Res. 1998 Sep;6(6):463-71
9865785
Chromosome Res. 2009;17(1):11-8
19105034
Cytogenetics. 1972;11(4):270-8
5072789
Front Biosci. 2008 May 01;13:4687-706
18508539
Chromosoma. 2007 Apr;116(2):135-45
17103220
Genetics. 1997 Sep;147(1):255-70
9286685
Mol Phylogenet Evol. 2004 Oct;33(1):197-213
15324848
Cytogenet Genome Res. 2003;103(1-2):169-72
15004482
Genetica. 2008 Jul;133(3):261-7
17899398
Chromosoma. 1991 Nov;101(2):123-32
1769277
EXS. 2001;(91):143-76
11301597
Chromosome Res. 2004;12(2):143-51
15053484
Cytogenet Cell Genet. 1973;12(5):297-304
4780767
Chromosome Res. 1999;7(4):289-95
10461874
Chromosome Res. 2007;15(7):917-30
17985203
Dev Dyn. 2002 Dec;225(4):384-91
12454917
Cytogenet Genome Res. 2003;103(1-2):173-84
15004483
Dev Dyn. 2009 Jun;238(6):1398-46
19441086
Chromosoma. 1985;91(3-4):172-84
3979176
Chromosome Res. 2002;10(7):571-7
12498346
Cytogenet Genome Res. 2007;116(1-2):110-2
17268187
Genome Dyn. 2006;2:138-153
18753776
BMC Genomics. 2008 Apr 14;9:168
18410676
Genomics. 2000 Feb 15;64(1):102-5
10708523
BMC Biol. 2007 Jul 25;5:31
17651506
Cytogenet Cell Genet. 1982;34(1-2):149-57
7151486
Chromosome Res. 2005;13(7):699-706
16235119