DREB2C interacts with ABF2, a bZIP protein regulating abscisic acid-responsive gene expression, and its overexpression affects abscisic acid sensitivity.
ABF2 is a basic leucine zipper protein that regulates abscisic acid (ABA)-dependent stress-responsive gene expression. We carried out yeast two-hybrid screens to isolate genes encoding ABF2-interacting proteins in Arabidopsis (Arabidopsis thaliana). Analysis of the resulting positive clones revealed that two of them encode an AP2 domain protein, which is the same as AtERF48/DREB2C. This protein, which will be referred to as DREB2C, could bind C-repeat/dehydration response element in vitro and possesses transcriptional activity. To determine its function, we generated DREB2C overexpression lines and investigated their phenotypes. The transgenic plants were ABA hypersensitive during germination and seedling establishment stages, whereas primary root elongation of seedlings was ABA insensitive, suggesting developmental stage dependence of DREB2C function. The DREB2C overexpression lines also displayed altered stress response; whereas the plants were dehydration sensitive, they were freezing and heat tolerant. We further show that other AP2 domain proteins, DREB1A and DREB2A, interact with ABF2 and that other ABF family members, ABF3 and ABF4, interact with DREB2C. Previously, others demonstrated that ABF and DREB family members cooperate to activate the transcription of an ABA-responsive gene. Our result implies that the cooperation of the two classes of transcription factors may involve physical interaction.
Lee
Sun-Ji
SJ
Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, South Korea.
Kang
Jung-Youn
JY
Park
Hee-Jin
HJ
Kim
Myoung Duck
MD
Bae
Min Seok
MS
Choi
Hyung-In
HI
Kim
Soo Young
SY
D000040:Abscisic Acid / Q000378:metabolism*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D003681:Dehydration; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018525:Germination; D006358:Hot Temperature; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D036226:Seedlings / Q000254:growth & development; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D000040:Abscisic Acid; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D018749:RNA, Plant; D014157:Transcription Factors; C508877:At1g45249 protein, Arabidopsis
Plant Cell. 2000 Apr;12(4):599-609
10760247
Biochem Biophys Res Commun. 2002 Jan 25;290(3):998-1009
11798174
Plant J. 1997 Jun;11(6):1237-51
9225465
Plant Physiol. 2002 Oct;130(2):639-48
12376631
Plant Physiol. 2002 Dec;130(4):2129-41
12481097
Plant J. 1999 Sep;19(6):679-89
10571853
Plant Physiol. 2004 Jul;135(3):1710-7
15247382
Plant J. 2003 Apr;34(2):137-48
12694590
Plant Biotechnol J. 2004 Sep;2(5):459-66
17168892
Plant Cell. 2005 Dec;17(12):3470-88
16284313
Trends Biochem Sci. 1995 Dec;20(12):506-10
8571452
Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):1035-40
9023378
Plant Mol Biol. 2004 May;55(1):135-47
15604670
BMC Genomics. 2007 Aug 01;8:260
17672917
Genes Dev. 2003 Apr 15;17(8):1043-54
12672693
Plant Cell Physiol. 2000 May;41(5):541-7
10929936
J Biol Chem. 2000 Jan 21;275(3):1723-30
10636868
Mol Cells. 2009 Oct 31;28(4):383-8
19830397
Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1988-93
16446457
Methods Mol Biol. 1998;82:259-66
9664431
Plant Physiol. 2006 Feb;140(2):411-32
16407444
Plant Cell. 2002;14 Suppl:S15-45
12045268
Plant Cell. 1998 Jun;10(6):1043-54
9634591
Plant Cell. 2002;14 Suppl:S165-83
12045276
Plant Mol Biol. 2005 Sep;59(2):253-67
16247556
Plant Physiol. 2002 Oct;130(2):688-97
12376636
Plant Cell. 1995 Mar;7(3):295-307
7734964
Biochem Biophys Res Commun. 2007 Oct 19;362(2):431-6
17716623
Plant J. 2003 Mar;33(5):949-56
12609035
Plant J. 2004 Oct;40(1):75-87
15361142
Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11632-7
11005831
Plant Physiol. 2004 Nov;136(3):3639-48
15516505
Plant Physiol. 2005 Nov;139(3):1185-93
16227468
Plant Mol Biol. 2004 Sep;56(1):29-55
15604727
Plant Cell. 1995 Jul;7(7):1099-1111
12242400
Plant Physiol. 2000 Dec;124(4):1854-65
11115899
Plant Mol Biol. 1998 Jun;37(3):425-35
9617810
Science. 1998 Apr 3;280(5360):104-6
9525853
Plant Mol Biol. 1994 Mar;24(5):701-13
8193295
Plant Cell Physiol. 2002 Jan;43(1):136-40
11828032
Trends Plant Sci. 2005 Feb;10(2):88-94
15708346
Plant Cell. 1996 Jul;8(7):1107-19
8768371
Plant Cell. 1994 Feb;6(2):251-64
8148648
Plant J. 2004 Feb;37(3):326-39
14731254
Plant Cell. 2006 May;18(5):1292-309
16617101
Plant Cell Environ. 2002 Feb;25(2):141-151
11841659
Plant J. 2002 Jun;30(6):679-89
12061899
Plant Cell. 1998 Aug;10(8):1391-406
9707537
Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18822-7
17030801
EMBO J. 1987 Dec 20;6(13):3901-7
3327686
Plant Cell. 2002 Feb;14(2):343-57
11884679
Plant Cell. 2005 Aug;17(8):2384-96
15994908
Plant Mol Biol. 2004 Jan;54(1):111-24
15159638
Plant Physiol. 2005 Dec;139(4):1750-61
16299177
Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15348-53
10611387
Plant Mol Biol. 2007 Jan;63(2):151-69
16977424