Discrete DNA sites regulate global distribution of meiotic recombination.
Homologous recombination is induced to high levels in meiosis, is initiated by Spo11-catalyzed DNA double-strand breaks (DSBs) and is clustered at hotspots that regulate its positioning in the genome. Recombination is required for proper chromosome segregation in meiosis and defects in its frequency or positioning cause chromosome mis-segregation and, consequently, congenital birth defects such as Down's syndrome. Therefore, elucidating how meiotic recombination is positioned is of fundamental and biomedical interest. Our integration of historical and contemporary advances in the field, plus the re-analysis of published microarray data on the genome-wide distribution of recombination supports a unifying model for such regulation. We posit that discrete DNA sequence motifs position and regulate essentially all recombination across the genome, in much the same way that DNA sites position and regulate transcription. Moreover, we illustrate the use of overlapping mechanisms for the regulation of transcription and meiotic recombination. Bound transcription factors induce histone modifications that position recombination at hotspots.
Wahls
Wayne P
WP
Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 4301 West Markham Street (slot 516), Little Rock, AR 72205-7199, USA. wahlswaynep@uams.edu
Davidson
Mari K
MK
D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D042421:Histone Code; D006657:Histones / Q000737:chemistry; D006801:Humans; D008540:Meiosis; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid
D006657:Histones; D004247:DNA
J Mol Biol. 1988 Dec 20;204(4):917-25
3221399
Eukaryot Cell. 2007 Nov;6(11):2072-80
17827346
Mol Cell Biol. 1995 Mar;15(3):1679-88
7862159
Mol Cell. 2002 Apr;9(4):847-55
11983175
Curr Biol. 2007 Dec 4;17(23):2003-12
18060788
Genetics. 2009 Jun;182(2):459-69
19363124
Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6621-5
8341678
EMBO J. 2004 May 5;23(9):1957-67
15071505
PLoS Genet. 2009 Dec;5(12):e1000753
19997497
EMBO J. 1998 Jul 15;17(14):4147-57
9670029
Nature. 2007 Oct 18;449(7164):851-61
17943122
PLoS Genet. 2007 Aug;3(8):e141
17722984
Mol Cell Biol. 2006 Feb;26(3):1014-27
16428454
Curr Genet. 1992 Feb;21(2):109-16
1568254
Genetics. 1971 Nov;69(3):317-37
17248549
Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):3955-60
17360459
Annu Rev Genet. 1999;33:603-754
10690419
Mol Cell Biol. 1991 Jan;11(1):289-98
1986226
Mol Biol Cell. 2008 Mar;19(3):1162-73
18199689
Cell. 1990 Jan 12;60(1):95-103
2295091
Mol Cell Biol. 1997 Dec;17(12):7008-18
9372932
Nature. 2008 Jul 24;454(7203):479-85
18615017
Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7446-51
9207111
Science. 2010 Feb 12;327(5967):876-9
20044541
EMBO J. 2009 Jan 21;28(2):99-111
19078966
Nature. 2002 Feb 21;415(6874):871-80
11859360
Genes Dev. 1994 Jul 15;8(14):1693-702
7958849
Genetics. 2005 Apr;169(4):1973-83
15716492
Bioessays. 2006 Jan;28(1):45-56
16369948
Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9755-9
1946399
Proc Natl Acad Sci U S A. 1966 Oct;56(4):1177-83
5230143
Cell. 2004 Aug 20;118(4):439-52
15315757
PLoS Genet. 2007 Jun;3(6):e100
17590084
Genetics. 1995 Jun;140(2):469-78
7498729
Nat Genet. 2008 Sep;40(9):1124-9
19165926
Nucleic Acids Res. 2008 May;36(9):2838-51
18375981
Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11383-90
11027339
EMBO J. 1991 Mar;10(3):681-6
2001681
Mol Cell Biol. 1998 Dec;18(12):7575-83
9819443
Mol Cell Biol. 2005 Oct;25(20):9054-62
16199881
Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11380-5
15280549
Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13765-70
9391101
EMBO J. 1991 Aug;10(8):2157-63
2065658
Mol Cell Biol. 1991 Jan;11(1):322-8
1986228
DNA Repair (Amst). 2008 Aug 2;7(8):1298-308
18515193
EMBO J. 2004 Apr 21;23(8):1792-803
14988732
Science. 2010 Feb 12;327(5967):836-40
20044539
Hum Mol Genet. 2007 Oct 15;16 Spec No. 2:R203-8
17911163
EMBO J. 2009 Sep 2;28(17):2616-24
19644444
PLoS One. 2009;4(5):e5533
19436749
PLoS One. 2008 Aug 06;3(8):e2887
18682829
PLoS Biol. 2007 Dec;5(12):e324
18076285
J Biol Chem. 2004 Dec 3;279(49):50857-63
15448137