title: Physiological response to lipid peroxidation in ischemia and reperfusion during carotid endarterectomy.
abstract: BACKGROUND
  In this study we aimed to assess lipid peroxidation during carotid endarterectomy by the formation of PUFA hydroperoxides (PUFAHP) and isoprostanes (IP) and concomitant peroxisomal beta-oxidation as a physiological mechanism to limit their concentration. Two markers of peroxisomal beta oxidation have been evaluated, formation of 2,3 dinor from IP and conjugated esadecadienoic acid (CD 16:2) from peroxisomal beta-oxidation of conjugated linoleic acid (CLA), an unusual fatty acid present in small concentration in our diet and preferentially beta-oxidised in peroxisomes.The study was conducted on 30 patients undergoing carotid endarterectomy. Blood samplings were performed before, during endarterectomy in the "ischemic phase", and 30 seconds, 30 minutes and 2 hours after reperfusion.
  
  
  RESULTS
  The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion. Interestingly, IP increased in a similar fashion of PUFAHP but never significantly. Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.
  
  
  CONCLUSIONS
  Our data show that lipid peroxidation takes place only in patients with high controlateral stenosis and within 2 hours occurs a physiological response aimed to decrease IP and PUFAHP by increasing their catabolism in peroxisomes.
authors.affiliation: Università degli Studi di Cagliari - Dipartimento di Biologia Sperimentale - Cittadella, Universitaria - 09042 Monserrato, Cagliari, Italy. banni@unica.it
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Banni
authors.forename: Roberto
authors.initials: R
authors.lastname: Montisci
authors.forename: Roberto
authors.initials: R
authors.lastname: Sanfilippo
authors.forename: Gabriele
authors.initials: G
authors.lastname: Finco
authors.forename: Daniela
authors.initials: D
authors.lastname: Sanna
authors.forename: Elena
authors.initials: E
authors.lastname: Giordano
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Murru
authors.forename: Lina
authors.initials: L
authors.lastname: Cordeddu
authors.forename: Gianfranca
authors.initials: G
authors.lastname: Carta
authors.forename: Donata
authors.initials: D
authors.lastname: Banni
authors.forename: Antonio
authors.initials: A
authors.lastname: Marchi
mesh_terms: D000222:Adaptation, Physiological; D000368:Aged; D002374:Catalase / Q000378:metabolism; D003251:Constriction, Pathologic; D016894:Endarterectomy, Carotid / Q000009:adverse effects*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005260:Female; D006801:Humans; D028421:Isoprostanes / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D020675:Peroxisomes / Q000378:metabolism; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism*
chemical_list: D005231:Fatty Acids, Unsaturated; D028421:Isoprostanes; D002374:Catalase
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