title: Assessment of cell-free DNA with microvascular complication of type II diabetes mellitus, using PCR and ELISA.
abstract: In order to assess the potential biochemical markers in the development, diagnosis, and prognosis of diabetic patient with microvascular complication represented with retinopathy, we analyzed the levels of cell-free DNA by two different techniques. The levels of cell-free GAPDH assayed by quantitative PCR were significantly higher in the plasma samples of diabetic patients with and without diabetic retinopathy than in those of the control group; thus, it is a better biomarker than nucleosomes assayed by ELISA in patients with type 2 diabetes for the early detection of development of microvascular complications as retinopathy.
authors.affiliation: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. shereeneltarhony@hotmail.com
authors.forename: Shereen A
authors.initials: SA
authors.lastname: El Tarhouny
authors.forename: Khaled M
authors.initials: KM
authors.lastname: Hadhoud
authors.forename: Magdy M
authors.initials: MM
authors.lastname: Ebrahem
authors.forename: Nashwa M
authors.initials: NM
authors.lastname: Al Azizi
mesh_terms: D000368:Aged; D002477:Cells; D004247:DNA / Q000097:blood / Q000235:genetics*; D048909:Diabetes Complications / Q000097:blood / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009707:Nucleosomes / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D009707:Nucleosomes; D004247:DNA