Coherent aperture-synthesis, wide-field, high-resolution holographic microscopy of biological tissue.
We show for the first time, to our knowledge, high-resolution wide-field images of biological samples recorded using coherent aperture-synthesis Fourier holography. To achieve this, we combined off-axis plane-wave polarized illumination with an axial sample rotation and polarization-sensitive collection of backscattered light. We synthesized 180 Fourier holograms using an efficient postdetection phase-matching correlation scheme. The result was an annular spatial frequency-space synthetic aperture (NA=0.93) with an effective area 25 times larger than that due to a single hologram. A high-resolution high-contrast microscopic reconstruction of biological tissue was computed over a sample area of 9 mm(2) from holograms acquired at 34 mm working distance.
Gutzler
Thomas
T
Optical and Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering,University of Western Australia, Crawley, Western Australia 6009, Australia. tgutzler@ee.uwa.edu.au
Hillman
Timothy R
TR
Alexandrov
Sergey A
SA
Sampson
David D
DD
D006696:Holography / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008198:Lymph Nodes / Q000166:cytology / Q000473:pathology; D008853:Microscopy / Q000379:methods*