title: The genetics of barley low-tillering mutants: low number of tillers-1 (lnt1).
abstract: Barley (Hordeum vulgare L.) carrying recessive mutations at the Low number of tillers1 (Lnt1) gene does not develop secondary tillers and only develops one to four tillers by maturity. Double mutant analysis determined that the lnt1 mutant was epistatic to five of the six low and high tillering mutants tested. Double mutants of lnt1 and the low tillering mutant intermedium-b (int-b) resulted in a uniculm plant, indicating a synergistic interaction and that Lnt and Int-b function in separate tillering pathways. RNA profiling identified 70 transcripts with either increased or decreased abundance in the lnt1 mutant compared to wild-type. One gene with reduced transcript levels in the lnt1 mutant was the BELL-like homeodomain transcription factor JuBel2. The JuBel2 allele in the lnt1.a mutant contained a frameshift mutation that eliminated most of the predicted polypeptide, indicating that the Lnt1 gene encodes JuBel2. Previous studies with the low-tillering mutant absent lower laterals (als) showed that the tillering phenotypes and genetic interactions of als and lnt1 with other tillering mutants were very similar. However, the transcriptomes were very different; many transcripts annotated as stress and defense response exhibited increased abundance in the als mutant. This difference suggests a functional separation between Als and Lnt1 in the genetic control of tillering.
authors.affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108, USA.
authors.forename: Timothy
authors.initials: T
authors.lastname: Dabbert
authors.forename: Ron J
authors.initials: RJ
authors.lastname: Okagaki
authors.forename: Seungho
authors.initials: S
authors.lastname: Cho
authors.forename: Shane
authors.initials: S
authors.lastname: Heinen
authors.forename: Jayanand
authors.initials: J
authors.lastname: Boddu
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Muehlbauer
mesh_terms: D001483:Base Sequence; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D001467:Hordeum / Q000033:anatomy & histology* / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018520:Plant Shoots / Q000166:cytology / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger
references.citation: BMC Genomics. 2009 Dec 04;10:582
references.pmid: 19961604
references.citation: Plant Physiol. 2004 Mar;134(3):960-8
references.pmid: 15020760
references.citation: Plant J. 2009 May;58(4):641-54
references.pmid: 19175771
references.citation: Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):290-5
references.pmid: 9874811
references.citation: Nature. 1997 Apr 3;386(6624):485-8
references.pmid: 9087405
references.citation: Cell. 1997 May 16;89(4):575-85
references.pmid: 9160749
references.citation: Curr Opin Plant Biol. 2005 Oct;8(5):506-11
references.pmid: 16054429
references.citation: Cell. 1989 Jan 27;56(2):225-9
references.pmid: 2643476
references.citation: Curr Biol. 2004 Jul 27;14(14):1232-8
references.pmid: 15268852
references.citation: Development. 2003 Sep;130(17):3941-50
references.pmid: 12874117
references.citation: Mol Cells. 2006 Feb 28;21(1):147-52
references.pmid: 16511358
references.citation: Development. 2002 Mar;129(5):1131-41
references.pmid: 11874909
references.citation: Genome. 1996 Oct;39(5):866-73
references.pmid: 8890517
references.citation: Plant Physiol. 2009 Jan;149(1):46-55
references.pmid: 19126694
references.citation: Nature. 2003 Apr 10;422(6932):618-21
references.pmid: 12687001
references.citation: Methods Mol Biol. 2000;132:365-86
references.pmid: 10547847
references.citation: Science. 2006 Jun 2;312(5778):1392-6
references.pmid: 16614172
references.citation: Plant Cell. 2003 Aug;15(8):1717-27
references.pmid: 12897247
references.citation: Curr Biol. 2003 Sep 16;13(18):1630-5
references.pmid: 13678595
references.citation: Plant J. 2007 Sep;51(6):1019-29
references.pmid: 17655651
references.citation: Genes Dev. 2003 May 1;17(9):1175-87
references.pmid: 12730136
references.citation: Genes Dev. 2003 Jun 15;17(12):1469-74
references.pmid: 12815068
references.citation: Theor Appl Genet. 2003 Mar;106(5):846-57
references.pmid: 12647059
references.citation: Theor Appl Genet. 2009 May;118(7):1351-60
references.pmid: 19241055
references.citation: Plant J. 2003 Feb;33(3):513-20
references.pmid: 12581309
references.citation: Plant Cell. 2007 Feb;19(2):458-72
references.pmid: 17307924
references.citation: Plant Mol Biol. 2006 Apr;60(6):843-54
references.pmid: 16724256
references.citation: Plant Cell. 2006 Mar;18(3):586-97
references.pmid: 16461581
references.citation: Plant Physiol. 2009 May;150(1):482-93
references.pmid: 19321710
references.citation: Curr Opin Plant Biol. 2007 Feb;10(1):21-5
references.pmid: 17140840
references.citation: Plant J. 2006 Dec;48(5):687-98
references.pmid: 17092317
references.citation: Nucleic Acids Res. 2006 Mar 02;34(4):1281-92
references.pmid: 16513846
references.citation: Mamm Genome. 2001 Dec;12(12):930-2
references.pmid: 11707780
references.citation: Annu Rev Plant Biol. 2008;59:253-79
references.pmid: 18444901
references.citation: Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11765-70
references.pmid: 13130077
references.citation: Plant Cell. 2005 Mar;17(3):746-59
references.pmid: 15705953
references.citation: Plant J. 2001 Jul;27(1):13-23
references.pmid: 11489179
references.citation: Plant Cell. 2009 Apr;21(4):1095-108
references.pmid: 19346465
references.citation: Plant Cell Physiol. 2005 Jan;46(1):79-86
references.pmid: 15659436
references.citation: Dev Biol. 2000 Feb 15;218(2):341-53
references.pmid: 10656774
references.citation: Mol Plant Microbe Interact. 2006 Apr;19(4):407-17
references.pmid: 16610744
references.citation: Plant Physiol. 2006 Nov;142(3):1014-26
references.pmid: 16980559