title: Expression levels of genes associated with oxygen utilization, glucose transport and glucose phosphorylation in hypoxia exposed Atlantic cod (Gadus morhua).
abstract: Expression level of genes associated with oxygen [cytochrome oxidase 1 (COX1) and myoglobin (Mb)] and glucose utilization [glucose transporters (GLUTs) and hexokinases (HKs)] along with metabolic indices were determined in Atlantic cod (Gadus morhua) subjected to an hypoxic challenge of <45% oxygen saturation for 24 days. There were two closely related HKs considered to be homologues of mammalian HKIs. HKIa and HKIb share 86% sequence identity and are both ubiquitously expressed. Mb was also expressed in many tissues with highest levels occurring in heart. Over the first 15 days of hypoxia there were transient increases in plasma lactate in hypoxic relative to normoxic fish associated with a significant decrease in liver glycogen. Over days 1-6, there were in ten of eleven cases, increased average (with a number of conditions being statistically significant) expression levels of GLUTs (1, 2, 4) and HKs (1a and b) in gill, heart, liver, and white muscle in hypoxic relative to normoxic fish. There were significant increases in COX1 and Mb expression levels in gill by day 24 but no changes in these aerobic indicators in heart or liver. Overall the data suggest a transient increase in genes associated with glucose utilization during the early part of the hypoxic challenge followed by alterations in gene expression in gill.
authors.affiliation: Ocean Sciences Centre, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1C 5S7.
authors.forename: J R
authors.initials: JR
authors.lastname: Hall
authors.forename: C E
authors.initials: CE
authors.lastname: Short
authors.forename: L H
authors.initials: LH
authors.lastname: Petersen
authors.forename: J
authors.initials: J
authors.lastname: Stacey
authors.forename: A K
authors.initials: AK
authors.lastname: Gamperl
authors.forename: W R
authors.initials: WR
authors.lastname: Driedzic
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D048389:Gadus morhua / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010100:Oxygen / Q000378:metabolism*; D010766:Phosphorylation; D010802:Phylogeny; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D005947:Glucose; D010100:Oxygen