Effect of stretching force on the cells of epithelial rests of malassez in vitro.
Background and Objective. The aim of this study was to investigate the behavior of cells from epithelial rest of Malassez (ERM) against stretching force. Material and Methods. ERM-cultured cells were stretched for 1 hour, at the cycle of 18% elongation for 1 second followed by 1-second relaxation. The cells without addition of stretching force were used as controls. The cells were observed by immunohistochmical staining using actin 0, 12, 24, 36, 48, and 72 hours. Furthermore, expressions of HSP70-, VEGF-, and OPN-mRNAs of cells were also evaluated using quantitative RT-PCR. Results. Actin filaments were randomly orientated in the cytoplasm in the control group, whereas in the stretching group, actin filaments were orientated comparatively parallel to the stretching direction. Expression of HSP70-mRNA in the stretching group was significantly higher than that of control group at 12, 24, 36 hours (P < .05). Expression of VEGF-mRNA in the stretching group was significantly higher than that of control group at 24, 36, 48, and 72 hours (P < .05). Expression of OPN-mRNA in the stretching group was significantly higher than that of control group at 12 and 24 hours (P < .05). Conclusion. ERM cells response against the stretching force by expressing HSP70, VEGF, and OPN.
Koshihara
Teruyoshi
T
Department of Crown & Bridge Prosthodontics, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba 261-8502, Japan.
Matsuzaka
Kenichi
K
Sato
Toru
T
Inoue
Takashi
T
Crit Rev Oral Biol Med. 2000;11(3):279-303
11021631
J Cell Sci. 1984 Jul;69:35-45
6490748
Laryngoscope. 1995 Dec;105(12 Pt 1):1300-4
8523981
Microsc Res Tech. 2003 Apr 1;60(5):491-502
12619125
Science. 1989 Dec 8;246(4935):1306-9
2479986
Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):233-5
7542074
J Orthop Res. 1994 Jan;12(1):70-8
8113944
J Periodontol. 2008 May;79(5):912-9
18454671
Eur J Oral Sci. 2003 Aug;111(4):332-8
12887399
Exp Nephrol. 1994 May-Jun;2(3):158-65
7922267
J Appl Physiol (1985). 2000 Dec;89(6):2498-507
11090608
Cell Biol Int. 2005 Feb;29(2):111-7
15774307
J Periodontal Res. 2006 Aug;41(4):245-52
16827716
Oral Surg Oral Med Oral Pathol. 1972 Dec;34(6):956-64
4509005
Exp Cell Res. 1980 Jun;127(2):470-4
7379874
J Periodontol. 1980 Aug;51(8):437-44
6931203
Orthod Craniofac Res. 2004 Feb;7(1):26-34
14989752
Dev Dyn. 1995 May;203(1):80-92
7647376
J Periodontal Res. 2003 Feb;38(1):51-6
12558937
J Dent Res. 2000 Sep;79(9):1712-6
11023268
Arch Oral Biol. 1998 Dec;43(12):987-97
9877330
J Biochem Mol Toxicol. 2003;17(3):161-8
12815612
Eur J Oral Sci. 2008 Aug;116(4):312-8
18705798
J Periodontal Res. 2005 Apr;40(2):110-7
15733145
Scand J Gastroenterol. 2006 Mar;41(3):312-7
16497619
Arch Oral Biol. 1998 Nov;43(11):899-906
9821513
World J Gastroenterol. 2005 Sep 28;11(36):5725-31
16237775
Eur J Cell Biol. 1986 Aug;41(2):339-46
3530766
Arch Oral Biol. 1979;24(3):199-203
289357
Nat Cell Biol. 2000 Aug;2(8):469-75
10934466
J Anat. 1993 Apr;182 ( Pt 2):153-62
8376190
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1996 Jun;81(6):703-9
8784903
FEBS Lett. 1993 May 24;323(1-2):1-3
8495719
Experientia. 1993 Aug 15;49(8):706-13
8359277
Crit Rev Oncog. 2000;11(2):135-46
11005509
Arch Oral Biol. 1989;34(3):179-85
2818267
Sci STKE. 2001 Jul 31;2001(93):re1
11752668
J Oral Rehabil. 2008 Dec;35(12):889-97
19090907
Arch Histol Cytol. 1989 Mar;52(1):61-70
2497762
J Periodontal Res. 2005 Oct;40(5):417-26
16105095
J Periodontol. 1986 Mar;57(3):164-71
3457140