Ornithinibacter aureus gen. nov., sp. nov., a novel member of the family Intrasporangiaceae.
A novel strain of the class Actinobacteria was isolated from a seawater sample collected in the South China Sea using modified R2A agar plates. The strain was a Gram-stain-positive, nonmotile, non-spore-forming, catalase-positive, irregular rod-shaped bacterium. The strain grew at 4-45 °C and pH 5.0-10.2, and tolerated 5% (w/v) NaCl. Based on its 16S rRNA gene sequence, the organism was related phylogenetically to members of the genera Fodinibacter (96.7% similarity), Lapillicoccus (96.5 %), Knoellia (95.0-95.8 %), Oryzihumus (95.6 %) and Humibacillus (95.6 %). The cell-wall contained L-ornithine as the major diagnostic diamino acid in the peptidoglycan. MK-8(H₄) was the predominant menaquinone. Major cellular fatty acids were iso-C₁₈:₁ω9c, iso-C₁₆:₀, iso-C₁₅:₀ and C₁₇:₀. The G+C content of the DNA was 69.6 mol%. Phenotypic and phylogenetic data revealed that this strain represents a novel species in a new genus of the family Intrasporangiaceae, for which the name Ornithinibacter aureus gen. nov., sp. nov. is proposed; the type strain of Ornithinibacter aureus is HB09001(T) (=CGMCC 1.10341(T) =DSM 23364(T)).
Xiao
Chuan
C
College of Environment and Plant Protection, Hainan University, Danzhou 571737, Hainan, PR China.|Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Ministry of Agriculture for Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
Huang
Huiqin
H
Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Ministry of Agriculture for Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
Ye
Jianjun
J
Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Ministry of Agriculture for Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
Wu
Xiaopeng
X
Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Ministry of Agriculture for Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
Zhu
Jun
J
Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Ministry of Agriculture for Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
Zhan
Baolin
B
Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Ministry of Agriculture for Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
Bao
Shixiang
S
Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Ministry of Agriculture for Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009952:Ornithine / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000032:analysis
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D009952:Ornithine