Proteomic alterations in progeny of irradiated human liver cells.
This study was designed to characterize the differential protein expression in the progeny of human liver cells surviving exposure to ionizing radiation. The progeny of irradiated cells were derived from a human liver cell line exposed to 0, 2, 4, or 6 Gy of (60)Co gamma-irradiation. Total protein of the cells was extracted by two-dimensional electrophoresis (2-DE) and analyzed with ImageMaster 2D Platinum software. In total, 42 differentially expressed proteins from the progeny of irradiated cells were screened, of which 17 were identified by matrix assistant laser desorption ion-top flight-mass spectrometry (MALDI-TOF-MS) analysis. There were 4 upregulated and 13 downregulated proteins detected. The upregulated expression of two proteins, mitochondrial heat-shock 60-kD protein (HSP60) and globin transcription factor 1 (GATA-1), was further confirmed by immunoblotting. Database search revealed that these differentially expressed proteins may function in cell cycle regulation, cytoskeleton maintenance, stress response, and tumor metastasis, indicating an effect of radiation-induced genomic instability (RIGI) in the progeny of irradiated cells. Analysis on functional roles of the screened proteins may provide insight into further mechanistic investigations underlying molecular events induced by RIGI.
Zuo
Ya-Hui
YH
School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou, China.
Wang
Xiao-Li
XL
Li
Jian-Guo
JG
Dang
Xu-Hong
XH
Wang
Zhong-Wen
ZW
Zhang
Su-Ping
SP
Tong
Jian
J
D002460:Cell Line; D018834:Chaperonin 60 / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D015536:Down-Regulation / Q000528:radiation effects; D015180:Electrophoresis, Gel, Two-Dimensional; D050982:GATA1 Transcription Factor / Q000378:metabolism; D042822:Genomic Instability / Q000528:radiation effects; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D020543:Proteome / Q000378:metabolism / Q000528:radiation effects*; D015854:Up-Regulation / Q000528:radiation effects
D018834:Chaperonin 60; D050982:GATA1 Transcription Factor; D020543:Proteome