title: Roseomonas pecuniae sp. nov., isolated from the surface of a copper-alloy coin.
abstract: Strain N75(T) was isolated from the surface of a copper-alloy 50 Euro cent coin collected from general circulation. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain N75(T) formed a distinct branch within the genus Roseomonas and placed it in the Alphaproteobacteria. Strain N75(T) showed 16S rRNA gene sequence similarities of 92.4-97.1  % to type strains of species of the genus Roseomonas. Strain N75(T) was a Gram-negative, non-spore-forming, non-motile coccoid, with an optimum growth temperature of about 30 °C; the strain did not grow at 5 or 37 °C. Strain N75(T) did not grow in medium containing NaCl. The major respiratory quinone was ubiquinone 10 (Q-10). The major fatty acids were unsaturated C₁₆:₁ω7c/C₁₆:₁ω6c and C₁₈:₁ω7c (around 70 % of the total fatty acids); the third most abundant fatty acid was the hydroxylated C₁₈:₁ 2-OH. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine and an unknown aminolipid. The DNA G+C content was 72.8 mol%. On the basis of the phylogenetic analysis and physiological and biochemical characteristics, we conclude that strain N75(T) represents a novel species of the genus Roseomonas, for which we propose the name Roseomonas pecuniae sp. nov. (type strain N75(T) =LMG 25481(T) =CIP 110074(T)).
authors.affiliation: IMAR, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: André
authors.initials: A
authors.lastname: Lopes
authors.affiliation: University of Nebraska-Lincoln School of Biological Sciences, E141 Beadle Center, Lincoln, NE 68588-0666, USA.|IMAR, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Christophe
authors.initials: C
authors.lastname: Espirito Santo
authors.affiliation: University of Nebraska-Lincoln School of Biological Sciences, E141 Beadle Center, Lincoln, NE 68588-0666, USA.
authors.forename: Gregor
authors.initials: G
authors.lastname: Grass
authors.affiliation: IMAR, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Chung
authors.affiliation: Department of Life Sciences, University of Coimbra, Apartado 3126, 3001-401 Coimbra, Portugal.|IMAR, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Paula V
authors.initials: PV
authors.lastname: Morais
mesh_terms: D000497:Alloys; D001482:Base Composition; D016000:Cluster Analysis; D003300:Copper; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis; D042082:Methylobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009717:Numismatics; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000497:Alloys; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003300:Copper