High-resolution melting analysis of BLU methylation levels in gastric, colorectal, and pancreatic cancers.
BLU was recently characterized as a novel tumor suppressor gene (TSG), and was epigenetically silenced in some tumor cell lines and primary tumor samples. High-resolution melting (HRM) analysis has been used as a novel tool for analysis of promoter methylation. We used HRM analysis to detect the methylation levels of BLU gene in 100 gastric, 100 colorectal, and 70 pancreatic cancers, and also in an equal number of adjacent normal tissues for all. The frequency of BLU methylation in all three types of cancer was significantly higher than that in normal tissues. And the expression levels of BLU were inversely correlated with methylation levels.
Shao
Yong
Y
Biomedical Research Institute, Shenzhen-PKU-HKUST Medical Center, Shenzhen, Guangdong, China.
Zhang
Wei
W
Zhang
Chao
C
Wu
Qi
Q
Yang
Hong
H
Zhang
Jie
J
Guan
Ming
M
Wan
Jun
J
Yu
Bo
B
D000367:Age Factors; D001483:Base Sequence; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D003598:Cytoskeletal Proteins; D019175:DNA Methylation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D009691:Nucleic Acid Denaturation; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
D003598:Cytoskeletal Proteins; D025521:Tumor Suppressor Proteins; C472720:ZMYND10 protein, human