Protection against Paracoccidioides brasiliensis infection conferred by the prophylactic administration of native and recombinant ArtinM.
We determined the prophylactic effect of both the d-mannose-binding lectin ArtinM extracted from the seeds of Artocarpus integrifolia (jackfruit) and its recombinant counterpart during the course of experimental paracoccidioidomycosis induced in BALB/c mice. Four experimental protocols of prophylaxis were employed to evaluate the most protective regimen of ArtinM administration. It was demonstrated that the best effect was obtained by administration of two ArtinM doses on days 10 and 3 before the challenge with Paracoccidioides brasiliensis. By following this protocol, the lungs of mice that received native or recombinant ArtinM exhibited reduced fungal burden and granuloma incidence. In addition, the protocol augmented contents of IL-12, IFN-gamma, TNF-alpha and NO. On the other hand, the control group consisting of untreated infected mice had higher pulmonary levels of IL-4 and IL-10. In conclusion, prophylaxis with ArtinM significantly reproduces the effect of its therapeutic administration, i.e, it confers resistance to P. brasiliensis infection in mouse models by promoting IL-12 production and favours Th1-immunity.
Coltri
Kely C
KC
Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Avenida Bandeirantes 3900, Ribeirão Preto, SP, Brazil.
Oliveira
Leandro L
LL
Ruas
Luciana P
LP
Vendruscolo
Patrícia E
PE
Goldman
Maria Helena
MH
Panunto-Castelo
Ademilson
A
Roque-Barreira
Maria-Cristina
MC
D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D031622:Artocarpus / Q000737:chemistry*; D018890:Chemoprevention / Q000379:methods*; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D037102:Lectins / Q000302:isolation & purification / Q000627:therapeutic use*; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000032:analysis; D010228:Paracoccidioides / Q000187:drug effects*; D010229:Paracoccidioidomycosis / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000235:genetics / Q000627:therapeutic use; D012639:Seeds / Q000737:chemistry
D000935:Antifungal Agents; D016207:Cytokines; D037102:Lectins; D011994:Recombinant Proteins; D009569:Nitric Oxide