Effect of IKVAV peptide nanofiber on proliferation, adhesion and differentiation into neurocytes of bone marrow stromal cells.
This study examined the effect of IKVAV peptide nanofiber on proliferation, adhesion and differentiation into neurocytes of bone marrow stromal cells (BMSCs). IKVAV Peptide-amphiphile was synthesized and purified. Then, hydrogen chloride was added to the diluted aqueous solutions of PA to induce spontaneous formation of nanofiber in vitro. The resultant samples was observed under transmission electron microscope. BMSCs were cultured with IKVAV peptide nanofiber. The effect of IKVAV nanofiber on the proliferation, adhesion and induction differentiation of BMSCs was observed by inverted microscopy, calcein-AM/PI staining, cell counting and immunofluorescence staining. The results demonstrated that IKVAV peptide-amphiphile could self-assemble to form nanofiber gel. BMSCs cultured in combination with IKVAV peptide nanofiber gel grew well and the percentage of live cells was over 90%. IKVAV peptide nanofiber gel exerted no influence on the proliferation of BMSCs and could promote the adhesion of BMSCs and raise the ratio of neurons when BMSCs were induced to differentiate into neurocytes. It is concluded that BMSCs could proliferate and adhere well and yield more neurons during when induced to differente into neurocytes on IKVAV peptide nanofiber gel.
Wu
Bin
B
Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. wubin1980@gmail.com
Zheng
Qixin
Q
Wu
Yongchao
Y
Guo
Xiaodong
X
Zou
Zhenwei
Z
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005782:Gels; D007797:Laminin / Q000494:pharmacology*; D057139:Nanofibers / Q000737:chemistry*; D009474:Neurons / Q000166:cytology*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
D005782:Gels; D007797:Laminin; D010446:Peptide Fragments; C083321:isoleucyl-lysyl-valyl-alanyl-valine
Neuropathology. 2003 Sep;23(3):169-80
14570283
IEEE Trans Nanobioscience. 2004 Mar;3(1):61-5
15382646
Science. 2003 Oct 3;302(5642):46-7
14526056
Science. 2004 Feb 27;303(5662):1352-5
14739465
Biopolymers. 2004;76(1):27-33
14997472
J Huazhong Univ Sci Technolog Med Sci. 2006;26(5):594-6
17219978
J Huazhong Univ Sci Technolog Med Sci. 2009 Feb;29(1):80-3
19224169
Biomaterials. 2004 Jan;25(1):77-83
14580911
FEBS Lett. 2002 Oct 23;530(1-3):48-52
12387864
J Huazhong Univ Sci Technolog Med Sci. 2009 Jun;29(3):296-9
19513609
Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5133-8
11929981
IEEE Trans Nanobioscience. 2002 Mar;1(1):24-8
16689218
J Neurosci Res. 2000 Aug 15;61(4):364-70
10931522
Cell Tissue Res. 1995 Feb;279(2):371-7
7895274
J Huazhong Univ Sci Technolog Med Sci. 2006;26(2):206-10
16850748
Biomaterials. 2007 Dec;28(34):5068-73
17706763
Science. 2004 Sep 24;305(5692):1923-4
15448260
Biomacromolecules. 2004 Sep-Oct;5(5):1933-9
15360308
Biomaterials. 2005 Mar;26(8):883-9
15353199
J Neurotrauma. 2006 Mar-Apr;23(3-4):496-507
16629632
J Am Chem Soc. 2003 Jun 18;125(24):7146-7
12797766
Stem Cells. 2004;22(4):487-500
15277695