Phenotypic and genetic characterization of antimicrobial profiles of Helicobacter pylori strains in Cuba.
The study evaluated the antibiotic resistance patterns of Helicobacter pylori strains against metronidazole and clarithromycin in a hospital in Havana, Cuba. Eighty-five percent, 22.5%, and 10% of 40 H. pylori strains investigated were resistant to metronidazole, ciprofloxacin, and clarithromycin respectively but all were susceptible to amoxicillin and tetracycline. RdxA truncation was found only in metronidazole-resistant strains. In such strains, reported are eight and two novel mutations in the rdxA and frxA genes respectively. Two-point mutations in the 23S rRNA genes of clarithromycin-resistant strains were detected. A high prevalence of metronidazole resistance was found in Cuban H. pylori strains. Mutations in the rdxA gene may contribute more significantly than frxA gene to the high level of resistance to metronidazole. This study supports the need to continue monitoring the antibiotic susceptibility in H. pylori in Cuba to guide the treatment of such infection.
Llanes
Rafael
R
Microbiology, Clinical and Epidemiology Branch, Institute Pedro Kouri, Havana, Cuba. llanes@ipk.sld.cu
Soria
Claudia
C
Nagashima
Shigeo
S
Kobayashi
Nobumichi
N
Gala
Angela
A
Guzmán
Daymi
D
Feliciano
Onelkis
O
Valdés
Lidunka
L
Gutiérrez
Oderay
O
Fernández
Heriberto
H
Llop
Alina
A
Wada
Akihiro
A
D000328:Adult; D000367:Age Factors; D000368:Aged; D000658:Amoxicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology; D017291:Clarithromycin / Q000494:pharmacology; D003462:Cuba; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005260:Female; D016480:Helicobacter pylori / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D012737:Sex Factors; D013045:Species Specificity; D013752:Tetracycline / Q000494:pharmacology; D055815:Young Adult
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D008795:Metronidazole; D002939:Ciprofloxacin; D000658:Amoxicillin; D013752:Tetracycline; D017291:Clarithromycin
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