Levels of follicular G-CSF and interleukin-15 appear as noninvasive biomarkers of subsequent successful birth in modified natural in vitro fertilization/intracytoplasmic sperm injection cycles.
OBJECTIVE
To explore oocyte competence for subsequent birth. The modified natural IVF/intracytoplasmic sperm injection (ICSI) cycle was used as an experimental model by measuring levels of cytokines, chemokines, and growth factors in individual follicular fluids (FF).


METHODS
A retrospective blinded study.


METHODS
European network of research, Embryo Implantation Control (EMBIC).


METHODS
Single FF from 83 women were analyzed during a modified natural IVF/ICSI cycle, and reproducibility of follicular composition was evaluated over two cycles for 15 patients.


METHODS
Each FF sample was blindly tested to assess levels of 26 factors by bead-based immunoassays.


METHODS
Each mediator was evaluated as a potential biomarker of subsequent birth by multivariate regression analysis.


RESULTS
A combination of both FF G-CSF and IL-15 was the optimal model to predict birth (AUC(ROC), 0.85). Birth rates per cycle were 48.9% (16/33) if two good-prognosis criteria were present (FF G-CSF>12 pg/mL and IL-15<7 pg/mL) and 8% (3/36) and 0% (0/14) if, respectively, one or none were present. FF G-CSF was significantly correlated over two cycles (r=.71), suggesting a possible prognostic value of its documentation.


CONCLUSIONS
Combined follicular G-CSF and IL-15 quantification appears as an efficient and noninvasive method to define oocyte competence for subsequent successful conception in modified natural IVF/ICSI cycles.
Lédée
Nathalie
N
INSERM U782, University Paris-Sud, and AP-HP, Service d’histologie, embryologie, cytogenetique, HôpitalAntoine Béclère,Clamart, France. nathalie-ledee@orange.fr
Frydman
René
R
Osipova
Agnessia
A
Taieb
Joelle
J
Gallot
Vanessa
V
Lombardelli
Letizia
L
Logiodice
Federica
F
Petitbarat
Marie
M
Fanchin
Renato
R
Chaouat
Gérard
G
Achour-Frydman
Nelly
N
Piccinni
Marie Pierre
MP
D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D019409:Interleukin-15 / Q000378:metabolism*; D015999:Multivariate Analysis; D006080:Ovarian Follicle / Q000378:metabolism; D011237:Predictive Value of Tests; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D011379:Prognosis; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic
D015415:Biomarkers; D019409:Interleukin-15; D016179:Granulocyte Colony-Stimulating Factor