title: Crystallization and preliminary X-ray study of the D-altritol oligonucleotide GTGTACAC.
abstract: In altritol nucleic acids (ANAs), the natural five-membered ribose ring of RNA is replaced by the six-membered D-altritol ring. ANAs are good candidates to act as siRNAs in the RNA-interference pathway. Crystals of the fully modified altritol self-complementary octamer GTGTACAC were grown by the hanging-drop vapour-diffusion technique at 289 K. Diffraction data were recorded on SLS beamline X06DA and processed to 3.0 A resolution. The crystals belonged to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = 25.05, c = 117.58 A.
authors.affiliation: Department of Chemistry, Biomolecular Architecture and BioMacS, Katholieke Universiteit Leuven, Belgium.
authors.forename: Margriet
authors.initials: M
authors.lastname: Ovaere
authors.forename: Arthur
authors.initials: A
authors.lastname: Van Aerschot
authors.forename: Mikhail
authors.initials: M
authors.lastname: Abramov
authors.forename: Piet
authors.initials: P
authors.lastname: Herdewijn
authors.forename: Luc
authors.initials: L
authors.lastname: Van Meervelt
mesh_terms: D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D009841:Oligonucleotides / Q000737:chemistry*; D013402:Sugar Alcohols / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D013402:Sugar Alcohols; C042890:altritol
references.citation: Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82
references.pmid: 16369096
references.citation: Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55
references.pmid: 15299926
references.citation: J Am Chem Soc. 2008 Feb 13;130(6):1979-84
references.pmid: 18198873
references.citation: Nucleic Acids Res. 2007;35(4):1064-74
references.pmid: 17264131
references.citation: Nat Rev Drug Discov. 2004 Apr;3(4):318-29
references.pmid: 15060527
references.citation: J Biomol Struct Dyn. 1991 Feb;8(4):801-11
references.pmid: 2059340
references.citation: J Am Chem Soc. 2002 Feb 13;124(6):928-33
references.pmid: 11829600
references.citation: Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):465-8
references.pmid: 11539196
references.citation: Nucleic Acids Res. 2005 Jan 14;33(1):439-47
references.pmid: 15653644
references.citation: Nucleic Acids Symp Ser (Oxf). 2008;(52):25-6
references.pmid: 18776235
references.citation: J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
references.pmid: 19461840
references.citation: Eur J Pharmacol. 2009 Mar 15;606(1-3):38-44
references.pmid: 19374843
references.citation: Nature. 2004 Sep 16;431(7006):343-9
references.pmid: 15372041
references.citation: Nucleic Acids Res. 2009 May;37(9):2867-81
references.pmid: 19282453
references.citation: Biophys J. 1992 Sep;63(3):751-9
references.pmid: 1384741
references.citation: Biochemistry. 1991 Apr 9;30(14):3567-76
references.pmid: 2012814
references.citation: Acta Crystallogr D Biol Crystallogr. 1993 Mar 1;49(Pt 2):282-91
references.pmid: 15299533
references.citation: J Am Chem Soc. 2007 Aug 1;129(30):9340-8
references.pmid: 17616127
references.citation: Biochemistry. 1989 Mar 21;28(6):2360-4
references.pmid: 2730868