title: Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression.
abstract: This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Genetic Science, Yonsei University College of Medicine, South Korea.
authors.forename: Jung-Yoon
authors.initials: JY
authors.lastname: Yoo
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Choi
authors.forename: HeeBum
authors.initials: H
authors.lastname: Kang
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jeongmin
authors.initials: J
authors.lastname: Lee
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Jun
authors.forename: Mi-Jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: Yoo-Hyun
authors.initials: YH
authors.lastname: Lee
authors.forename: Ok-Hee
authors.initials: OH
authors.lastname: Lee
authors.forename: Ho-Geun
authors.initials: HG
authors.lastname: Yoon
mesh_terms: D000818:Animals; D015870:Gene Expression; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D006657:Histones / Q000235:genetics*; D000099186:Histone Deacetylase 3
chemical_list: D006655:Histone Deacetylases; D006657:Histones; D000099186:Histone Deacetylase 3