title: Immunoglobulin G subclass responses to recombinant Em18 in the follow-up of patients with alveolar echinococcosis in different clinical stages.
abstract: In this study, we compared the sequential responses of immunoglobulin G (IgG) subclasses to the diagnostic antigen Em18 in sera from patients with alveolar echinococcosis. A total of 225 sera from 36 patients at different clinical stages according to the WHO-PNM staging system were tested. The antibody responses were measured for cohorts with resected and unresected parasitic lesions by enzyme-linked immunosorbent assays (ELISA). Total IgG and, to a lesser extent, IgG4 antibody levels against Em18 correlated with all PNM stages before treatment, whereas levels of IgG2 were low and IgG3 was undetectable. Antibody kinetics, however, depended on the treatment rather than on the PNM stage. For some patients, after curative surgery, IgG1 antibodies dropped below the cutoff earlier than other antibodies, followed by total IgG and IgG4 within 18 months. For some patients with recurrences after surgery, IgG1 and IgG4 reappeared, whereas patients with unresectable lesions but stable disease showed steady declines in the levels of all antibodies, and IgG1 became undetectable in some patients. Additional testing of IgE responses to Em18 showed constantly low levels at all stages and in all cohorts.
authors.affiliation: Institute of Hygiene and Microbiology, Josef-Schneider-Str. 2, 97080 Würzburg, Germany. dtappe@hygiene.uni-wuerzburg.de
authors.forename: Dennis
authors.initials: D
authors.lastname: Tappe
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sako
authors.forename: Sonoyo
authors.initials: S
authors.lastname: Itoh
authors.forename: Matthias
authors.initials: M
authors.lastname: Frosch
authors.forename: Beate
authors.initials: B
authors.lastname: Grüner
authors.forename: Peter
authors.initials: P
authors.lastname: Kern
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000909:Antibodies, Helminth / Q000097:blood* / Q000145:classification; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D004445:Echinococcosis, Pulmonary / Q000276:immunology* / Q000469:parasitology / Q000503:physiopathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005858:Germany; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000145:classification; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C117889:Em18 antigen; D007074:Immunoglobulin G; D011994:Recombinant Proteins
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