Intracellular imaging of organelles with new water-soluble benzophenoxazine dyes.
Five new water-soluble derivatives of Nile Red 1-5 were prepared. These benzophenoxazine dyes fluoresce between 640 and 667 nm with quantum yields of 0.17-0.33 in pH 7.4 phosphate buffer, and at slightly shorter wavelengths and higher quantum yields in EtOH. Two dyes, 3 and 4 permeated into Clone 9 cells and selectively stained mitochondria and golgi, respectively.
Jose
Jiney
J
Department of Chemistry, Texas A&M University, Box 30012, College Station, TX 77841, USA.
Loudet
Aurore
A
Ueno
Yuichiro
Y
Barhoumi
Rola
R
Burghardt
Robert C
RC
Burgess
Kevin
K
D000818:Animals; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D015388:Organelles / Q000737:chemistry* / Q000378:metabolism*; D010078:Oxazines / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D011789:Quantum Theory; D012995:Solubility; D013194:Staining and Labeling; D014018:Tissue Distribution; D014867:Water / Q000737:chemistry*
D005456:Fluorescent Dyes; D010078:Oxazines; D014867:Water