Antimicrobial activity of super-oxidised water against oral microorganisms.
OBJECTIVE
The radical anion of oxygen (O(-)) is extremely oxidative and shows high reactivity. In this study, the antibacterial activity of water super-oxidised water containing high concentration of O(-) (O(-)-water) was tested against cultured planktonic cells of cariogenic bacteria, periodontopathic bacteria and Candida albicans.


METHODS
O(-)-water was prepared using the AOE-750 (Oxy Japan Corporation, Japan) and its antibacterial activity against pure culture of Streptococcus sobrinus, Porphyromonas gingivalis, Prevotella intermedia, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum and C. albicans evaluated. Each oral microorganism (10(4) to 10(8)CFU/ml) was exposed to three concentrations of O(-)-water at room temperature or 37 degrees C for 15s to 24h.


RESULTS
Exposure to O(-)-water resulted in a bactericidal effect against all cariogenic and periodontopathic bacteria tested. No significant fungicidal effect was observed on C. albicans, however.


CONCLUSIONS
The results demonstrate that O(-)-water exerts an antibacterial effect on cariogenic and periodontopathic bacteria.
Yamada
Kiyotaka
K
Department of Clinical Oral Health Science, Tokyo Dental College, Tokyo, Japan.
Yama
Mitsuru
M
Takaku
Yuichiro
Y
Kakizawa
Takashi
T
Kimizuka
Ryuta
R
Okuda
Katsuji
K
Kato
Tetsuo
T
D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D015169:Colony Count, Microbial; D003731:Dental Caries / Q000382:microbiology; D019551:Dental Disinfectants / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010518:Periodontitis / Q000382:microbiology; D010933:Plankton / Q000187:drug effects
D000891:Anti-Infective Agents, Local; D019551:Dental Disinfectants; C118085:superoxidized water; D006861:Hydrogen Peroxide