Altered expression of NFkappaB in ex vivo differentiated dendritic cells from the aged subjects: implications in immunotherapy.
Dendritic cells are the key initiators and organizers of the immune response. They are specialized antigen-presenting cells whose immunogenicity leads to the induction of antigen-specific immune responses. They are therefore ideal vehicles to deliver strong immunogenic signals to eliminate tumors. Dendritic cells can dictate the polarization of T cell responses by virtue of the nature of their cytokine secretion. They are also critical for maintenance of peripheral self-tolerance. Because of their key role in these central processes, dendritic cells are increasingly being used for immunotherapy of various diseases. This has been greatly aided by the development of methods to derive dendritic cells ex vivo from human adult stem cells and other progenitors. Here we describe methods to assay the activity of transcription factor NFkappaB in the ex vivo derived dendritic cells and discuss the probable effects of the altered expression of NFkappaB on the functions of these dendritic cells.
Agrawal
Anshu
A
Division of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA, USA. aagrawal@uci.edu
D000367:Age Factors; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D007167:Immunotherapy; D009000:Monocytes / Q000166:cytology; D016328:NF-kappa B / Q000032:analysis / Q000378:metabolism*; D010766:Phosphorylation; D051996:Transcription Factor RelA / Q000378:metabolism
D016328:NF-kappa B; D051996:Transcription Factor RelA