Proteomic analysis of in vitro newly excysted juveniles from Fasciola hepatica.
Fasciolosis is a world-wide distributed zoonotic disease affecting several herbivores, and represents an important factor of economic loss in animal meat producing industries. In addition, specific risk factors and geographic areas for Fasciola hepatica human infection have been heavily reported recently. Several aspects related with this disease, e.g., drug resistance and prevention through vaccination, have yet to be solved. After ingestion, the infective stage for the vertebrate host-metacercariae - hatch in duodenum and the newly excysted juveniles (NEJ) penetrate the intestinal wall. The identification of proteins expressed by NEJ and specifically those found in the host-parasite interface could help understanding the first steps of animal and human infection by F. hepatica. Here we use a proteomic approach to identify a set of proteins enriched at the host-parasite interface from in vitro NEJ by applying liquid chromatography and tandem mass spectrometry (LC-MS/MS) analysis. Using this approach, we identified numerous proteins related with several biological processes of the parasite. In addition, we characterize one of the identified molecules, the 14-3-3z protein, and demonstrate its association with the outer structures of NEJ and its presence in both somatic and secretory components from the parasite. The NEJ proteins described here, together with those previously described by others, could provide new insights into the biology of the parasite and its relationship with the vertebrate host at the beginning of the infection.
Hernández-González
A
A
IRNASA, CSIC, Cordel de Merinas, 40-52, 37008 Salamanca, Spain.
Valero
M L
ML
del Pino
M Sánchez
MS
Oleaga
A
A
Siles-Lucas
M
M
D000818:Animals; D002853:Chromatography, Liquid; D005210:Fasciola hepatica / Q000737:chemistry*; D015801:Helminth Proteins / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D037521:Virulence Factors / Q000032:analysis
D015801:Helminth Proteins; D008565:Membrane Proteins; D020543:Proteome; D037521:Virulence Factors