title: Organophosphorus hydrolase (OpdB) of Lactobacillus brevis WCP902 from kimchi is able to degrade organophosphorus pesticides.
abstract: Lactobacillus brevis WCP902 that is capable of biodegrading chlorpyrifos was isolated from kimchi. The opdB gene cloned from this strain revealed 825 bp, encoding 274 aa, and an enzyme molecular weight of about 27 kDa. OpdB contains the same Gly-X-Ser-X-Gly motif found in most bacterial and eukaryotic esterase, lipase, and serine hydrolases, yet it is a novel member of the GDSVG family of esterolytic enzymes. Its conserved serine residue, Ser82, is significantly involved with enzyme activity that may have application for removing some pesticides. Optimum organophosphorus hydrolase (OpdB) activity appeared at pH 6.0 and 35 degrees C and during degradation of chlorpyrifos, coumaphos, diazinon, methylparathion, and parathion.
authors.affiliation: Division of Applied Life Science (BK21 Program), Gyeongsang National University, Chinju, Korea.
authors.forename: Shah Md Asraful
authors.initials: SM
authors.lastname: Islam
authors.forename: Renukaradhya K
authors.initials: RK
authors.lastname: Math
authors.forename: Kye Man
authors.initials: KM
authors.lastname: Cho
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Lim
authors.forename: Su Young
authors.initials: SY
authors.lastname: Hong
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Myoung Geun
authors.initials: MG
authors.lastname: Yun
authors.forename: Ji Joong
authors.initials: JJ
authors.lastname: Cho
authors.forename: Han Dae
authors.initials: HD
authors.lastname: Yun
mesh_terms: D043303:Aryldialkylphosphatase / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D052196:Levilactobacillus brevis / Q000201:enzymology*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010575:Pesticides / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D009943:Organophosphorus Compounds; D010575:Pesticides; D043303:Aryldialkylphosphatase