title: Protein kinase A regulatory subunit distribution in medulloblastoma.
abstract: BACKGROUND
  Previous studies showed a differential distribution of the four regulatory subunits of cAMP-dependent protein kinases inside the brain, that changed in rodent gliomas: therefore, the distribution of these proteins inside the brain can give information on the functional state of the cells. Our goal was to examine human brain tumors to provide evidence for a differential distribution of protein kinase A in different tumors.
  
  
  METHODS
  The distribution of detergent insoluble regulatory (R1 and R2) and catalytic subunits of cAMP dependent kinases was examined in pediatric brain tumors by immunohistochemistry and fluorescent cAMP analogues binding.
  
  
  RESULTS
  R2 is organized in large single dots in medulloblastomas, while it has a different appearance in other tumors. Fluorescent cAMP labelling was observed only in medulloblastoma.
  
  
  CONCLUSIONS
  A different distribution of cAMP dependent protein kinases has been observed in medulloblastoma.
authors.affiliation: Department of Human Anatomy and Physiology, University of Padova, Padua, Italy. carla.mucignat@unipd.it
authors.forename: Carla
authors.initials: C
authors.lastname: Mucignat-Caretta
authors.forename: Luca
authors.initials: L
authors.lastname: Denaro
authors.forename: Marco
authors.initials: M
authors.lastname: Redaelli
authors.forename: Domenico
authors.initials: D
authors.lastname: D'Avella
authors.forename: Antonio
authors.initials: A
authors.lastname: Caretta
mesh_terms: D000293:Adolescent; D002384:Catalysis; D002528:Cerebellar Neoplasms / Q000201:enzymology*; D002648:Child; D002675:Child, Preschool; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D008527:Medulloblastoma / Q000201:enzymology*; D021122:Protein Subunits; D014407:Tumor Cells, Cultured
chemical_list: D021122:Protein Subunits; D017868:Cyclic AMP-Dependent Protein Kinases
references.citation: J Comp Neurol. 2002 Sep 30;451(4):324-33
references.pmid: 12210127
references.citation: Development. 2001 Jun;128(11):1971-81
references.pmid: 11493520
references.citation: Anticancer Res. 1997 Jan-Feb;17(1A):301-6
references.pmid: 9066668
references.citation: Neuro Oncol. 2008 Dec;10(6):958-67
references.pmid: 18708342
references.citation: J Clin Oncol. 2008 Jun 10;26(17):2821-7
references.pmid: 18539960
references.citation: J Chem Neuroanat. 2004 Jun;27(3):201-12
references.pmid: 15183205
references.citation: J Neurocytol. 2001 Nov;30(11):885-94
references.pmid: 12373096
references.citation: Annu Rev Pathol. 2008;3:341-65
references.pmid: 18039127
references.citation: Pharmacol Ther. 2000 Aug-Sep;87(2-3):199-226
references.pmid: 11008001
references.citation: Cancer Res. 2007 Apr 15;67(8):3871-7
references.pmid: 17413002
references.citation: Ann N Y Acad Sci. 2006 Jul;1070:120-8
references.pmid: 16888153
references.citation: Int J Cancer. 2005 Oct 20;117(1):82-9
references.pmid: 15880586
references.citation: Dev Biol. 2008 Jan 1;313(1):359-70
references.pmid: 18036580
references.citation: Neuron. 1999 Jan;22(1):103-14
references.pmid: 10027293
references.citation: Front Biosci. 2008 May 01;13:5257-78
references.pmid: 18508585
references.citation: Neuro Oncol. 2008 Dec;10(6):1040-60
references.pmid: 18676356
references.citation: J Biol Chem. 2002 Apr 19;277(16):13443-8
references.pmid: 11834733
references.citation: Oncogene. 2004 Nov 18;23(54):8847-56
references.pmid: 15480415
references.citation: Cancer Res. 2007 Jan 15;67(2):651-8
references.pmid: 17234775
references.citation: Genes Chromosomes Cancer. 2000 Jan;27(1):44-51
references.pmid: 10564585
references.citation: Apoptosis. 2003 Dec;8(6):579-86
references.pmid: 14574064