Alkaloids from roots of Stephania rotunda and their cholinesterase inhibitory activity.
In the course of screening plants used in folk medicine as memory enhancers, a 70% ethanolic extract of Stephania rotunda roots showed significant AChE inhibitory activity. Repeated column chromatography led to the isolation of a new protoberberine alkaloid, which we named stepharotudine (1), and seven known compounds (2-8). The chemical structures of the isolated compounds were elucidated based on extensive 1D and 2D NMR spectroscopic data. Compounds 1-8 were investigated in vitro for their anticholinesterase activity using a rat cortex AChE enzyme.
Hung
Tran Manh
TM
College of Pharmacy, Catholic University of Daegu, Gyeongbuk, Korea.
Dang
Nguyen Hai
NH
Kim
Jin Cheol
JC
Jang
Han-Su
HS
Ryoo
Sung-Woo
SW
Lee
Jeong Hyung
JH
Choi
Jae Sue
JS
Bae
Kihwan
K
Min
Byung Sun
BS
D000110:Acetylcholinesterase / Q000302:isolation & purification; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001600:Berberine Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001921:Brain / Q000201:enzymology; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D058851:GPI-Linked Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D031607:Stephania / Q000737:chemistry*
D000470:Alkaloids; D001600:Berberine Alkaloids; D002800:Cholinesterase Inhibitors; D058851:GPI-Linked Proteins; C557381:stepharotudine; D000110:Acetylcholinesterase; C548246:Ache protein, rat