Characterization of the mouse CP27 promoter and NF-Y mediated gene regulation.
The cp27 gene is a highly conserved and unique gene with important roles related to craniofacial organogenesis. The present study is a first analysis of the CP27 promoter and its regulation. Here, we have cloned the promoter of the mouse cp27 gene, examined its transcriptional activity, and identified transcription factor binding sites in the proximal promoter region. Two major transcription start sites were mapped adjacent to exon 1. Promoter function analysis of the 5' flanking region by progressive 5' deletion mutations localized transcription repression elements between -1993bp and -969bp and several positive elements between -968bp and the preferred transcription start site. EMSA and functional studies indicated two function-cooperative CCAAT boxes and identified the NF-Y transcription factor as the CCAAT activator controlling transactivation of the CP27 promoter. In addition, this study demonstrated that for its effective binding and function, NF-Y required not only the minimal DNA segment length identified by deletion studies, but also a defined nucleotide sequence in the distal 3' flanking region of the CP27 proximal promoter CCAAT box. These results provide a basis for our understanding of the specific regulation of the cp27 gene in the NF-Y-mediated gene transcription network.
Luan
Xianghong
X
Brodie Laboratory for Craniofacial Genetics, University of Illinois at Chicago, 801 South Paulina Street, Chicago, IL 60612, USA.
Ito
Yoshihiro
Y
Zhang
Youbin
Y
Diekwisch
Thomas G H
TG
D024509:3' Flanking Region; D016475:3T3 Cells; D024506:5' Flanking Region; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D023081:CCAAT-Binding Factor / Q000235:genetics*; D002874:Chromosome Mapping; D016326:Extracellular Matrix Proteins; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D024363:Transcription Initiation Site
D023081:CCAAT-Binding Factor; C121022:Cfdp1 protein, mouse; D016326:Extracellular Matrix Proteins; D009687:Nuclear Proteins; D011506:Proteins; C402244:nuclear factor Y
Mech Dev. 1997 Oct;67(2):111-23
9392510
J Biol Chem. 1997 Oct 17;272(42):26562-72
9334236
Mol Biol Evol. 2007 Mar;24(3):814-26
17205977
Nucleic Acids Res. 1998 Mar 1;26(5):1135-43
9469818
Gene. 1999 Oct 18;239(1):15-27
10571030
J Biol Chem. 1997 Jan 31;272(5):2801-7
9006920
J Biol Chem. 1999 Aug 13;274(33):23242-8
10438498
J Histochem Cytochem. 2002 Apr;50(4):583-6
11897812
PLoS One. 2008 Apr 23;3(4):e2047
18431504
Mol Cell Biol. 2008 Mar;28(6):2047-58
18212061
Cell Prolif. 2002 Aug;35(4):207-19
12153613
J Biol Chem. 2003 Aug 15;278(33):30435-40
12771133
J Biol Chem. 1995 Apr 14;270(15):8851-9
7721793
Cancer Res. 2003 Dec 1;63(23):8167-72
14678971
Gene. 1999 Jul 22;235(1-2):19-30
10415329
J Biol Chem. 1994 Aug 5;269(31):20119-25
8051100
Adv Dent Res. 1995 Nov;9(3 Suppl):12
8934940
J Biol Chem. 1992 Apr 25;267(12):8478-84
1569098
PLoS One. 2008 Apr 30;3(4):e2066
18446193
J Biol Chem. 2009 Jan 30;284(5):3136-3147
19047043
J Biol Chem. 2000 Apr 21;275(16):12273-80
10766866
Development. 1993 Jul;118(3):665-90
7915668
Anat Rec. 1996 Jun;245(2):131-50
8769659
Genome Res. 2003 May;13(5):773-80
12727897
Biochem J. 1991 Dec 15;280 ( Pt 3):761-7
1764039
J Biol Chem. 2005 Apr 8;280(14):13606-15
15647281
Nucleic Acids Res. 2006;34(21):6272-85
17098936
J Biol Chem. 2003 Jan 10;278(2):1336-45
12401788
J Biol Chem. 2003 Feb 28;278(9):6642-50
12482752
J Biol Chem. 2000 Jan 28;275(4):2979-85
10644769
Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):3948-53
10759542
Gene. 2008 Apr 30;413(1-2):42-8
18316164
EMBO J. 1998 Nov 2;17(21):6300-15
9799238
Nucleic Acids Res. 2008 Nov;36(20):6592-607
18940868
J Biol Chem. 2006 Jun 9;281(23):16090-8
16595692
Cancer Cell. 2006 Sep;10(3):191-202
16959611
Biochem Biophys Res Commun. 1999 Nov 19;265(2):439-47
10558886
Oncogene. 2004 May 20;23(23):4084-8
15064732
EMBO J. 1995 Sep 15;14(18):4514-22
7556094
Nucleic Acids Res. 1992 Apr 11;20(7):1769-75
1349743
Gene. 2003 Jul 17;312:225-37
12909359
Gene Expr. 2005;12(3):193-212
16128003
FEBS Lett. 2003 Feb 27;537(1-3):58-62
12606031
Brain Res Mol Brain Res. 1999 Nov 10;73(1-2):172-80
10581410
Biotechniques. 1995 Jul;19(1):54-5
7669296
J Biol Chem. 2008 Jul 11;283(28):19359-70
18424784
J Biol Chem. 1999 Nov 26;274(48):34327-36
10567409
Biochem Biophys Res Commun. 2009 Feb 6;379(2):356-61
19103169
Cell. 1987 Sep 11;50(6):863-72
3476205
Proc Natl Acad Sci U S A. 1988 Oct;85(19):7322-6
3174636
Mol Cell Biol. 2005 May;25(9):3737-51
15831478
Gene. 2002 Apr 3;287(1-2):141-7
11992732