title: Isolation of sulfur reducing and oxidizing bacteria found in contaminated drywall.
abstract: Drywall from China has been reported to release sulfur producing products which are corrosive to metals, result in noxious odors, and represent a significant health risk. It has been reported that these emissions produce medical symptoms such as respiratory or asthma type problems, sinusitis, gastrointestinal disorders, and vision problems in home owners and their household pets. We report here a method of identifying a causative agent for these emissions by sampling affected gypsum wallboard and subjecting those samples to Real Time Polymerase Chain Reaction [RT-PCR] studies. Specific DNA probes and primers have been designed and patented that detect a specific iron and sulfur reducing bacterium (i.e., Thiobacillus ferrooxidans). One hundred percent of affected drywall samples obtained from homes located in the southeastern United States tested positive for the presence of T. ferrooxidans. All negative controls consisting of unaffected wallboard and internal controls, Geotrichum sp., tested negative within our limits of detection.
authors.affiliation: RealTime Laboratories, LLC, Dallas, TX 79234, USA. mscmd@cox.net <mscmd@cox.net>
authors.forename: Dennis G
authors.initials: DG
authors.lastname: Hooper
authors.forename: John
authors.initials: J
authors.lastname: Shane
authors.forename: David C
authors.initials: DC
authors.lastname: Straus
authors.forename: Kaye H
authors.initials: KH
authors.lastname: Kilburn
authors.forename: Vincent
authors.initials: V
authors.lastname: Bolton
authors.forename: John S
authors.initials: JS
authors.lastname: Sutton
authors.forename: Frederick T
authors.initials: FT
authors.lastname: Guilford
mesh_terms: D015342:DNA Probes / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D060888:Real-Time Polymerase Chain Reaction; D013455:Sulfur / Q000737:chemistry / Q000378:metabolism*; D013855:Thiobacillus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014934:Wood / Q000382:microbiology*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D013455:Sulfur; D007501:Iron
references.citation: Toxicol Ind Health. 1995 Mar-Apr;11(2):185-97
references.pmid: 7491634
references.citation: Environ Geochem Health. 2007 Aug;29(4):303-12
references.pmid: 17508258
references.citation: J Clin Microbiol. 2003 Mar;41(3):1304-6
references.pmid: 12624073
references.citation: Appl Environ Microbiol. 1990 Jun;56(6):1620-6
references.pmid: 16348205
references.citation: J Biosci Bioeng. 2000;90(2):193-8
references.pmid: 16232841
references.citation: Appl Environ Microbiol. 2000 May;66(5):2166-74
references.pmid: 10788396
references.citation: Sci Total Environ. 2006 Aug 15;367(1):1-22
references.pmid: 16650463
references.citation: South Med J. 1997 Oct;90(10):997-1006
references.pmid: 9347810
references.citation: J Bacteriol. 1992 Jun;174(12):4189-92
references.pmid: 1597434