title: Analysis of the central pair microtubule complex in Chlamydomonas reinhardtii.
abstract: The central pair microtubule complex in Chlamydomonas flagella has been well characterized as a regulator of flagellar dynein activity, but many aspects of this regulation depend on specific interactions between the asymmetric central pair structure and radial spokes, which appear symmetrically arranged along all nine outer doublet microtubules. Relationships between central pair-radial spoke interactions and dynein regulation have been difficult to understand because the Chlamydomonas central pair is twisted in vivo and rotates during bend propagation. Here we describe genetic and biochemical methods of dissecting the Chlamydomonas central pair and electron microscopic methods useful to determine structure-function relationships in this complex.
authors.affiliation: Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Mitchell
authors.forename: Brandon
authors.initials: B
authors.lastname: Smith
mesh_terms: D020418:Algal Proteins / Q000378:metabolism*; D054468:Axoneme / Q000648:ultrastructure; D001671:Biochemistry / Q000379:methods*; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D055785:Gene Knockdown Techniques; D005821:Genetic Techniques; D015151:Immunoblotting; D008870:Microtubules / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D009154:Mutation / Q000235:genetics
chemical_list: D020418:Algal Proteins