title: Membrane solubilisation and reconstitution by octylglucoside: comparison of synthetic lipid and natural lipid extract by isothermal titration calorimetry.
abstract: We have studied the solubilisation and reconstitution of lipid membranes composed of either synthetic phosphatidylcholine or Escherichia. coli polar lipid extract by the non-ionic detergent octylglucoside. For both lipid systems, composition-dependent transformations of unilamellar vesicles into micelles or vice versa were followed by high-sensitivity isothermal titration calorimetry. Data obtained over a range of detergent and lipid concentrations could be rationalised in terms of a three-stage phase separation model involving bilayer, bilayer/micelle coexistence, and micellar ranges, yielding the detergent/lipid phase diagrams and the bilayer-to-micelle partition coefficients of both detergent and lipid. The most notable difference between the lipids investigated was a substantial widening of the bilayer/micelle coexistence range for E. coli lipid, which was due to an increased preference of the detergent and a decreased affinity of the lipid for the micellar phase as compared with the bilayer phase. These effects on the bilayer-to-micelle partition coefficients could be explained by the high proportion in E. coli membranes of lipids possessing negative spontaneous curvature, which hampers both their transfer into strongly curved micellar structures as well as the insertion of detergent into condensed bilayers.
authors.affiliation: Leibniz Institute of Molecular Pharmacology (FMP), Robert-Rössle-Str. 10, 13125 Berlin, Germany.
authors.forename: Oxana O
authors.initials: OO
authors.lastname: Krylova
authors.forename: Nadin
authors.initials: N
authors.lastname: Jahnke
authors.forename: Sandro
authors.initials: S
authors.lastname: Keller
mesh_terms: D002151:Calorimetry; D004926:Escherichia coli / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry* / Q000302:isolation & purification; D044367:Phase Transition; D010713:Phosphatidylcholines / Q000737:chemistry*; D012995:Solubility; D013696:Temperature
chemical_list: D005960:Glucosides; D008081:Liposomes; D008563:Membrane Lipids; D010713:Phosphatidylcholines; C018619:octyl-beta-D-glucoside