title: Umbilical cord stem cell seeding on fast-resorbable calcium phosphate bone cement.
abstract: Tissue engineering offers immense promise for bone regeneration. Human umbilical cord mesenchymal stem cells (hUCMSCs) can be collected without invasive procedures required for bone marrow MSCs. The objective of this study was to investigate the physical properties and the differentiation capacity of hUCMSCs on calcium phosphate cement (CPC) scaffolds with improved dissolution/resorption rates. CPC consisted of tetracalcium phosphate and dicalcium phosphate anhydrous, with various tetracalcium phosphate/dicalcium phosphate anhydrous ratios. At 1/3 ratio, CPC had a dissolution rate 40% faster than CPC control at 1/1. The faster-resorbable CPC had strength and modulus similar to CPC control. Their strength and modulus exceeded the reported values for cancellous bone, and were much higher than those of hydrogels and injectable polymers for cell delivery. hUCMSCs attached to the nano-apatitic CPC and proliferated rapidly. hUCMSCs differentiated into the osteogenic lineage, with significant increases in alkaline phosphatase activity, osteocalcin, collagen I, and osterix gene expression. In conclusion, in this study we reported that hUCMSCs attaching to CPC with high dissolution/resorption rate showed excellent proliferation and osteogenic differentiation. hUCMSCs delivered via high-strength CPC have the potential to be an inexhaustible and low-cost alternative to the gold-standard human bone marrow mesenchymal stem cells. These results may broadly impact stem-cell-based tissue engineering.
authors.affiliation: Department of Endodontics, Prosthodontics, and Operative Dentistry, University of Maryland Dental School, Baltimore, Maryland 21201, USA. hxu@umaryland.edu
authors.forename: Hockin H K
authors.initials: HH
authors.lastname: Xu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Michael S
authors.initials: MS
authors.lastname: Detamore
authors.forename: Shozo
authors.initials: S
authors.lastname: Takagi
authors.forename: Laurence C
authors.initials: LC
authors.lastname: Chow
mesh_terms: D001843:Bone Cements / Q000737:chemistry* / Q000378:metabolism*; D002130:Calcium Phosphates / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates
references.citation: J Biomed Mater Res. 2002;63(1):1-9
references.pmid: 11787022
references.citation: J Biomed Mater Res. 2001 May 1;58(3):295-301
references.pmid: 11319744
references.citation: Biomaterials. 2007 Jan;28(2):299-306
references.pmid: 16982090
references.citation: Biomaterials. 2007 Oct;28(28):4078-90
references.pmid: 17576009
references.citation: Stem Cells. 2007 Nov;25(11):2886-95
references.pmid: 17690177
references.citation: Stem Cells. 2007 Jun;25(6):1384-92
references.pmid: 17332507
references.citation: J Tissue Eng Regen Med. 2009 Jul;3(5):398-404
references.pmid: 19434662
references.citation: J Biomed Mater Res A. 2008 Dec 1;87(3):760-9
references.pmid: 18200545
references.citation: J Biomed Mater Res B Appl Biomater. 2010 Apr;93(1):93-105
references.pmid: 20091907
references.citation: Tissue Eng. 2007 Mar;13(3):493-500
references.pmid: 17362133
references.citation: Biomaterials. 2006 Jun;27(17):3249-55
references.pmid: 16490250
references.citation: Biomaterials. 2009 May;30(14):2675-82
references.pmid: 19187958
references.citation: J Biomed Mater Res. 2001 Dec 5;57(3):457-66
references.pmid: 11523041
references.citation: Biomaterials. 2005 Apr;26(12):1337-48
references.pmid: 15482821
references.citation: J Biomed Mater Res. 2002 Jul;61(1):47-52
references.pmid: 12001245
references.citation: Biomacromolecules. 2009 Jul 13;10(7):1810-7
references.pmid: 19469498
references.citation: Biomaterials. 2003 Nov;24(24):4337-51
references.pmid: 12922147
references.citation: Biomaterials. 2008 Feb;29(6):675-82
references.pmid: 17996293
references.citation: Biomaterials. 2007 Oct;28(28):4091-7
references.pmid: 17586040
references.citation: Biomaterials. 2001 Mar;22(6):511-21
references.pmid: 11219714
references.citation: J Biomed Mater Res. 1998 Winter;43(4):428-32
references.pmid: 9855201
references.citation: Bone. 2009 Jul;45(1):27-41
references.pmid: 19306956
references.citation: Biomaterials. 1999 Dec;20(23-24):2287-303
references.pmid: 10614935
references.citation: Annu Rev Biomed Eng. 1999;1:19-46
references.pmid: 11701481
references.citation: J Craniofac Surg. 1997 Sep;8(5):367-72
references.pmid: 9482077
references.citation: Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11298-303
references.pmid: 17581871
references.citation: J Res Natl Inst Stand Technol. 2008 Nov 1;113(6):311-320
references.pmid: 19779581
references.citation: Biomaterials. 2006 Dec;27(36):6102-10
references.pmid: 16860387
references.citation: J Biomed Mater Res A. 2004 Mar 15;68(4):725-34
references.pmid: 14986327
references.citation: Biotechnol Bioeng. 2005 Aug 5;91(3):261-84
references.pmid: 15929124
references.citation: J Biomed Mater Res A. 2007 Nov;83(2):434-45
references.pmid: 17465019
references.citation: Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2494-9
references.pmid: 16477029
references.citation: Tissue Eng. 2007 Aug;13(8):2003-10
references.pmid: 17518722
references.citation: J Mater Sci Mater Med. 2000 May;11(5):305-11
references.pmid: 15348028
references.citation: Biomaterials. 2005 Jan;26(3):303-10
references.pmid: 15262472
references.citation: Biomaterials. 2005 May;26(13):1553-63
references.pmid: 15522757
references.citation: J Biomed Mater Res B Appl Biomater. 2003 May 15;65(2):245-51
references.pmid: 12687717
references.citation: J Dent Res. 2006 Nov;85(11):966-79
references.pmid: 17062735
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20641-6
references.pmid: 19095799
references.citation: Stem Cells. 2009 Jan;27(1):126-37
references.pmid: 18832592
references.citation: Biomaterials. 2007 Feb;28(6):1152-62
references.pmid: 17092555
references.citation: Biomaterials. 2005 Nov;26(33):6423-9
references.pmid: 15964620
references.citation: Stem Cells. 2004;22(7):1330-7
references.pmid: 15579650
references.citation: J Biomed Mater Res. 2001 Oct;57(1):113-8
references.pmid: 11416857
references.citation: Biomaterials. 2007 Jan;28(1):66-77
references.pmid: 16963119
references.citation: J Appl Biomater. 1991 Fall;2(3):187-208
references.pmid: 10149083
references.citation: J Biomed Mater Res A. 2008 Apr;85(1):218-27
references.pmid: 17688280
references.citation: Biomaterials. 2004 Jul;25(16):3187-99
references.pmid: 14980414
references.citation: Biomaterials. 2010 Feb;31(5):840-7
references.pmid: 19850337
references.citation: Biomaterials. 2001 May;22(9):963-72
references.pmid: 11311015
references.citation: J Bone Miner Res. 2005 May;20(5):848-57
references.pmid: 15824858
references.citation: Biomaterials. 2005 Nov;26(32):6323-34
references.pmid: 15919111
references.citation: Biomaterials. 2005 Mar;26(9):971-7
references.pmid: 15369685
references.citation: Biomaterials. 2002 Jan;23(1):193-202
references.pmid: 11763861