The archaeo-eukaryotic GINS proteins and the archaeal primase catalytic subunit PriS share a common domain.
Primase and GINS are essential factors for chromosomal DNA replication in eukaryotic and archaeal cells. Here we describe a previously undetected relationship between the C-terminal domain of the catalytic subunit (PriS) of archaeal primase and the B-domains of the archaeo-eukaryotic GINS proteins in the form of a conserved structural domain comprising a three-stranded antiparallel beta-sheet adjacent to an alpha-helix and a two-stranded beta-sheet or hairpin. The presence of a shared domain in archaeal PriS and GINS proteins, the genes for which are often found adjacent on the chromosome, suggests simple mechanisms for the evolution of these proteins.


METHODS
This article was reviewed by Zvi Kelman (nominated by Michael Galperin) and Kira Makarova.
Swiatek
Agnieszka
A
Centre for Biomolecular Sciences, School of Biology, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK. as2326@st-andrews.ac.uk
Macneill
Stuart A
SA
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics; D020134:Catalytic Domain; D019915:DNA Primase / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D019843:Archaeal Proteins; D011506:Proteins; D019915:DNA Primase
Proc Natl Acad Sci U S A. 2008 Jun 10;105(23):8102-7
18535141
FEBS J. 2007 Mar;274(5):1340-51
17286576
Trends Biochem Sci. 2000 Nov;25(11):572-6
11084371
J Biol Chem. 2007 Nov 16;282(46):33444-33451
17893144
Nucleic Acids Res. 2009 Jan;37(Database issue):D32-6
18927115
Biochem J. 2010 Jan 15;425(3):489-500
20070258
Genes Cells. 2003 Dec;8(12):913-23
14750947
Nat Struct Biol. 2001 Jan;8(1):57-61
11135672
Nucleic Acids Res. 2005 Jul 15;33(12):3875-96
16027112
Trends Cell Biol. 2007 Jun;17(6):271-8
17467990
Nat Struct Mol Biol. 2005 Dec;12(12):1137-44
16273105
Mol Cell. 2010 Jan 29;37(2):247-58
20122406
J Biol Chem. 2008 Jan 18;283(3):1601-1609
17986447
Bioinformatics. 2007 Nov 1;23(21):2947-8
17846036
Bioinformatics. 2000 Jun;16(6):566-7
10980157
Curr Protoc Bioinformatics. 2006 Jul;Chapter 5:Unit 5.5
18428766
Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10236-10241
16798881
Genes Dev. 2007 Jun 1;21(11):1316-21
17545466
Nat Rev Microbiol. 2008 Mar;6(3):245-52
18274537
Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12685-90
17652513
Biochemistry. 1999 Sep 28;38(39):12899-907
10504261
Nat Struct Mol Biol. 2007 May;14(5):388-96
17417653
Annu Rev Biochem. 2001;70:39-80
11395402
EMBO Rep. 2006 May;7(5):539-45
16485022
Nucleic Acids Res. 2005 Aug 16;33(14):4626-38
16106042
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
9396791
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712
Trends Biochem Sci. 2005 Aug;30(8):437-44
16002295
Nat Struct Mol Biol. 2007 Sep;14(9):875-7
17704817