title: Amplex UltraRed enhances the sensitivity of fluorimetric pyruvate detection.
abstract: Pyruvate, which is produced in the final step of glycolysis, participates in anabolic metabolism, catabolic metabolism, and signal transduction. We have recently reported a new sensitive and selective fluorimetric assay for pyruvate measurement using Amplex Red as a fluorogenic dye. However, the fluorescence of the reaction product, resorufin, is pH dependent, which limits the sensitivity of the assay at pH 6.7. In this Note, we evaluate Invitrogen's new dye, Amplex UltraRed, as a fluorogenic agent for pyruvate determination. Our results show that Amplex UltraRed improves the performance of the assay, providing brighter fluorescence and enhanced sensitivity.
authors.affiliation: Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI 48105, USA.
authors.forename: Aiping
authors.initials: A
authors.lastname: Zhu
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero
authors.forename: Howard R
authors.initials: HR
authors.lastname: Petty
mesh_terms: D005456:Fluorescent Dyes / Q000378:metabolism*; D005470:Fluorometry / Q000379:methods*; D010078:Oxazines / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D010078:Oxazines; C470430:Amplex Red; D019289:Pyruvic Acid
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