title: Antiplatelet antibodies in cases of Glanzmann's thrombasthenia with and without a history of multiple platelet transfusion.
abstract: Antiplatelet antibodies are known to be present in a wide spectrum of patients, which include chronic Idiopathic Thrombocytopenic Purpura (ITP), infections, etc., including Glanzmann's thrombasthenia (GT) patients who receive multiple platelet transfusions. The presence of natural antibodies to platelet receptors is not studied in cases of GT. We studied the antiplatelet antibodies in 23 patients with GT, 15 of which had received multiple transfusions and eight that had not received transfusions, along with 50 cases of chronic ITP. The prevalence and specificity of platelet-bound antibodies were detected by inhibition assays using O-group platelets on flow cytometry. The mean antiplatelet antibodies in 15 patients of GT who had not received transfusions and eight patients with multiple transfusions was 8427 + 2131.88 and 9038 + 2856 antibodies/platelet, respectively, while in case of the 50 ITP patients studied, it was 22166 + 5616 antibodies/platelet (Normal Range 1500-3200 antibodies/platelet). We conclude that GT patients who have not received transfusions may develop antiplatelet antibodies to the missing/abnormal receptor. Whether this is due to a molecular mimicry or due to some other mechanism needs to be explored.
authors.affiliation: Institute of Immunohaematology (ICMR), KEM Hospital, Parel, Mumbai, India.
authors.forename: Kanjaksha
authors.initials: K
authors.lastname: Ghosh
authors.forename: B
authors.initials: B
authors.lastname: Kulkarni
authors.forename: S
authors.initials: S
authors.lastname: Shetty
authors.forename: S
authors.initials: S
authors.lastname: Nair
references.citation: J Autoimmun. 1990 Aug;3(4):473-83
references.pmid: 2222752
references.citation: Circulation. 1997 Sep 2;96(5):1424-31
references.pmid: 9315527
references.citation: Thromb Haemost. 2000 Nov;84(5):779-83
references.pmid: 11127855
references.citation: J Acquir Immune Defic Syndr Hum Retrovirol. 1998 Apr 15;17(5):385-90
references.pmid: 9562039
references.citation: Hum Antibodies Hybridomas. 1992 Apr;3(2):93-106
references.pmid: 1633270
references.citation: J Lab Clin Med. 2003 Aug;142(2):113-20
references.pmid: 12960958
references.citation: J Infect Dis. 2002 Sep 15;186(6):743-51
references.pmid: 12198607
references.citation: Transfusion. 1994 Nov-Dec;34(11):955-60
references.pmid: 7526491
references.citation: Br J Haematol. 1981 May;48(1):47-57
references.pmid: 6454435
references.citation: J Obstet Gynaecol Res. 1997 Jun;23(3):247-50
references.pmid: 9255036
references.citation: J Thromb Haemost. 2003 Oct;1(10):2255-6
references.pmid: 14521621
references.citation: Semin Hematol. 2000 Jul;37(3):219-21
references.pmid: 10942215
references.citation: Blood. 1999 Jul 1;94(1):46-51
references.pmid: 10381497