Genomic alterations of primary tumor and blood in invasive ductal carcinoma of breast.
BACKGROUND
Genomic alterations are important events in the origin and progression of various cancers, with DNA copy number changes associated with progression and treatment response in cancer. Array CGH is potentially useful in the identification of genomic alterations from primary tumor and blood in breast cancer patients. The aim of our study was to compare differences of DNA copy number changes in blood and tumor tissue in breast cancer.


METHODS
DNA copy number changes in blood were compared to those in tumor tissue using array-comparative genomic hybridization in samples obtained from 30 breast cancer patients. The relative degree of chromosomal changes was analyzed using log2 ratios and data was validated by real-time polymerase chain reaction.


RESULTS
Forty-six regions of gains present in more than 30% of the tissues and 70 regions of gains present in more than 30% of blood were identified. The most frequently gained region was chromosome 8q24. In total, agreement of DNA copy numbers between primary tumor and blood was minimal (Kappa = 0.138, p < 0.001).


CONCLUSIONS
Although there was only a slight agreement of DNA copy number alterations between the primary tumor and the blood samples, the blood cell copy number variation may have some clinical significance as compared to the primary tumor in IDC breast cancer patients.
Bae
Ja Seong
JS
Department of Surgery, The Catholic University, Seoul, Korea.
Choi
Jin Soo
JS
Baik
Seung Ho
SH
Park
Woo Chan
WC
Song
Byung Joo
BJ
Kim
Jeong Soo
JS
Lim
Young
Y
Jung
Sang Seol
SS
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D055028:Comparative Genomic Hybridization; D056915:DNA Copy Number Variations / Q000235:genetics*; D004273:DNA, Neoplasm / Q000097:blood*; D005260:Female; D015894:Genome, Human; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011379:Prognosis
D004273:DNA, Neoplasm
Clin Genet. 2007 Nov;72(5):441-7
17935507
Genes Chromosomes Cancer. 1997 Jan;18(1):1-7
8993975
Int J Cancer. 2004 Oct 20;112(1):26-32
15305372
Br J Cancer. 2006 Jan 30;94(2):333-7
16333309
Cancer Res. 2004 Dec 1;64(23):8541-9
15574760
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4834-8
3014513
Nat Genet. 2001 Nov;29(3):263-4
11687795
J Pathol. 2005 Feb;205(3):388-96
15682439
Nat Genet. 1999 Sep;23(1):41-6
10471496
Mol Cancer Res. 2007 Sep;5(9):881-90
17855657
Genomics. 2006 Feb;87(2):298-306
16271290
Breast Cancer Res Treat. 2003 Apr;78(3):289-98
12755488
J Mol Diagn. 2005 Feb;7(1):65-71
15681476
Genes Chromosomes Cancer. 1997 Dec;20(4):399-407
9408757
Nature. 2002 Jan 31;415(6871):530-6
11823860
Mol Cancer Ther. 2003 Oct;2(10):971-84
14578462
Cancer Cell. 2002 Feb;1(1):37-47
12086886
Br J Cancer. 2004 Mar 22;90(6):1120-4
15026788
Curr Opin Genet Dev. 2007 Jun;17(3):182-92
17467974
BMC Cancer. 2006 Apr 18;6:96
16620391
Neoplasia. 2004 May-Jun;6(3):240-7
15153336
Genes Chromosomes Cancer. 1999 Jun;25(2):115-22
10337995
Int J Cancer. 2006 Mar 15;118(6):1556-64
16187286
Cancer Res. 2003 Dec 15;63(24):8861-8
14695203
Genes Chromosomes Cancer. 1998 Mar;21(3):177-84
9523192
Nat Genet. 2003 Aug;34(4):369-76
12923544
Asian Pac J Cancer Prev. 2005 Jan-Mar;6(1):16-21
15780025
Int J Cancer. 1997 Oct 21;74(5):513-7
9355973
Am J Pathol. 2000 Apr;156(4):1189-96
10751344
Cancer Res. 2003 Sep 1;63(17):5266-73
14500357
Nature. 1998 Dec 17;396(6712):643-9
9872311
Cell Oncol. 2004;26(5-6):307-17
15623941
J Clin Invest. 2008 Feb;118(2):640-50
18172554
J Matern Fetal Neonatal Med. 2004 Jul;16(1):55-9
15370084
Oncogene. 1987;1(4):423-30
3330785
Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10869-74
11553815
Nat Genet. 1998 Oct;20(2):207-11
9771718
Aquat Toxicol. 2003 Oct 29;65(2):141-57
12946615
Br J Cancer. 2005 Aug 8;93(3):346-54
16052224
Cancer Genet Cytogenet. 2005 Nov;163(1):23-9
16271952
Clin Breast Cancer. 2003 Feb;3(6):381-90
12636883
Leukemia. 2005 Jun;19(6):1005-9
15800673
Exp Hematol. 2002 Jun;30(6):503-12
12063017