title: Ex vivo expansion of human circulating myogenic progenitors on cluster-assembled nanostructured TiO2.
abstract: Ex vivo expansion of hematopoietic stem cells has been explored in the fields of stem cell biology, gene therapy and clinical transplantation. Recently, we demonstrated the existence of a circulating myogenic progenitor expressing the CD133 antigen. The relative inability of circulating CD133+ stem cells to reproduce themselves ex vivo imposes substantial limitations on their use for clinical applications in muscular dystrophies. Here we report that the use of cluster-assembled nanostructured titanium dioxide (ns-TiO(2)) substrates, in combination with cytokine enriched medium, enables high-level expansion of circulating CD133+ stem cells in vitro. Furthermore, we demonstrate that expanded circulating CD133+ stem cells retain their in vitro capacity to differentiate into myogenic cells. The exploitation of cluster-assembled ns-TiO(2) substrates for the expansion of CD133+ stem cells in vitro could therefore make the clinical application of these stem cells for the treatment of muscle diseases practical.
authors.affiliation: Stem Cell Laboratory, Department of Neurological Sciences, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, Università di Milano, Padiglione Ponti, Via F. Sforza 35, 20122 Milano, Italy.
authors.forename: Marzia
authors.initials: M
authors.lastname: Belicchi
authors.forename: Silvia
authors.initials: S
authors.lastname: Erratico
authors.forename: Paola
authors.initials: P
authors.lastname: Razini
authors.forename: Mirella
authors.initials: M
authors.lastname: Meregalli
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cattaneo
authors.forename: Emanuela
authors.initials: E
authors.lastname: Jacchetti
authors.forename: Andrea
authors.initials: A
authors.lastname: Farini
authors.forename: Chiara
authors.initials: C
authors.lastname: Villa
authors.forename: Nereo
authors.initials: N
authors.lastname: Bresolin
authors.forename: Laura
authors.initials: L
authors.lastname: Porretti
authors.forename: Cristina
authors.initials: C
authors.lastname: Lenardi
authors.forename: Paolo
authors.initials: P
authors.lastname: Milani
authors.forename: Yvan
authors.initials: Y
authors.lastname: Torrente
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006410:Hematopoiesis / Q000187:drug effects; D006801:Humans; D016130:Immunophenotyping; D051379:Mice; D018625:Microscopy, Atomic Force; D032342:Muscle Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D024510:Muscle Development / Q000187:drug effects; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D016207:Cytokines; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; C009495:titanium dioxide; D014025:Titanium