A domain level interaction network of amyloid precursor protein and Abeta of Alzheimer's disease.
The primary constituent of the amyloid plaque, beta-amyloid (Abeta), is thought to be the causal "toxic moiety" of Alzheimer's disease. However, despite much work focused on both Abeta and its parent protein, amyloid precursor protein (APP), the functional roles of APP and its cleavage products remain to be fully elucidated. Protein-protein interaction networks can provide insight into protein function, however, high-throughput data often report false positives and are in frequent disagreement with low-throughput experiments. Moreover, the complexity of the CNS is likely to be under represented in such databases. Therefore, we curated the published work characterizing both APP and Abeta to create a protein interaction network of APP and its proteolytic cleavage products, with annotation, where possible, to the level of APP binding domain and isoform. This is the first time that an interactome has been refined to domain level, essential for the interpretation of APP due to the presence of multiple isoforms and processed fragments. Gene ontology and network analysis were used to identify potentially novel functional relationships among interacting proteins.
Perreau
Victoria M
VM
Neuroproteomics and Neurogenomics Platform, National Neurosciences Facility, The University of Melbourne, Parkville, VIC, Australia. vperreau@unimelb.edu.au
Orchard
Sandra
S
Adlard
Paul A
PA
Bellingham
Shayne A
SA
Cappai
Roberto
R
Ciccotosto
Giuseppe D
GD
Cowie
Tiffany F
TF
Crouch
Peter J
PJ
Duce
James A
JA
Evin
Genevieve
G
Faux
Noel G
NG
Hill
Andrew F
AF
Hung
Ya Hui
YH
James
Simon A
SA
Li
Qiao-Xin
QX
Mok
Su San
SS
Tew
Deborah J
DJ
White
Anthony R
AR
Bush
Ashley I
AI
Hermjakob
Henning
H
Masters
Colin L
CL
D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D049490:Systems Biology
D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor