Investigation of supplemental ultraviolet-B-induced changes in antioxidative defense system and leaf proteome in radish (Raphanus sativus L. cv Truthful): an insight to plant response under high oxidative stress.
Impact of supplemental UV-B (sUV-B) has been investigated on photosynthetic pigments, antioxidative enzymes, metabolites, and protein profiling of radish plants under realistic field conditions. Exposure of sUV-B leads to oxidative damage in plants. However, plants possess a number of UV-protection mechanisms including a stimulation of antioxidant defense system. It caused alteration in reactive oxygen species metabolism primarily by decreasing catalase activity vis-à-vis enhanced activities of other enzymatic (superoxide dismutase, ascorbate peroxidase, and glutathione reductase) and non-enzymatic (ascorbic acid) antioxidants. Qualitative analysis of samples also showed significant reductions in photosynthetic pigments and protein content. After sUV-B exposure, protein profile showed differences mainly at eight points--126.8, 84.8, 71.9, 61.5, 47.8, 40.6, 38.9, and 17.5 kDa, whereas protein(s) of 38.9 kDa showed increment. Results of the present investigation clearly showed the adverse effect of sUV-B on total biomass at final harvest.
Singh
Akansha
A
Ecology Research Circle, Department of Botany, Banaras Hindu University, Varanasi, 221005, India.
Sarkar
Abhijit
A
Singh
Suruchi
S
Agrawal
S B
SB
D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases; D002338:Carotenoids / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D010544:Peroxidases / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism / Q000528:radiation effects; D010940:Plant Proteins / Q000378:metabolism; D031224:Raphanus / Q000033:anatomy & histology / Q000378:metabolism / Q000528:radiation effects; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays
D000975:Antioxidants; D010940:Plant Proteins; D002734:Chlorophyll; D002338:Carotenoids; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase
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