title: Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography.
abstract: Six volatile compounds, curdione (1), curcumol (2), germacrone (3), curzerene (4), 1,8-cineole (5) and beta-elemene (6), were successfully isolated from the essential oil of Curcuma wenyujin by high-performance centrifugal partition chromatography using a nonaqueous two-phase solvent system consisting of petroleum ether-acetonitrile-acetone (4:3:1 v/v/v). A total of 8 mg of curdione (1), 4 mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), 9 mg of 1,8-cineole (5) and 17 mg of beta-elemene (6) were isolated from the essential oil (300 mg) in 500 min. Their structures were determined by comparison of their retention times and MS data with those of the authentic samples as well as NMR spectroscopic analysis.
authors.affiliation: Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, P. R. China.
authors.forename: Yuan-Ye
authors.initials: YY
authors.lastname: Dang
authors.forename: Xiao-Cen
authors.initials: XC
authors.lastname: Li
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Zhang
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Li
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Wang
mesh_terms: D002498:Centrifugation; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D030024:Curcuma / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008956:Models, Chemical; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
chemical_list: D009822:Oils, Volatile; D010936:Plant Extracts; D012997:Solvents