[Application of BIOMED-2 primers in immunoglobulin gene rearrangement analysis of ocular adnexal lymphoma: a pilot study].
OBJECTIVE
To assess the practical value of BIOMED-2 primers in the diagnosis of ocular adnexal lymphoma by PCR.


METHODS
DNA was extracted from 63 formalin-fixed paraffin-embedded (FFPE) ocular adnexal lymphoma specimens. The DNA quality was evaluated by PCR-based amplification of housekeeping gene beta-actin. IgH(B) and IgK(B) primers of BIOMED-2 standardized clonality analysis system were used to evaluate the immunoglobin gene rearrangements. PCR products were analyzed using capillary electrophoresis and GeneScan software.


RESULTS
76.2% (48/63) of FFPE samples produced amplifiable DNA for detection of Ig gene rearrangements.Positive detection rates by BIOMED-2 IgH(B) and IgK(B) primers were 79.2% (38/48) and 68.8% (33/48), respectively, with a combined positive detection rate of 91.7% (44/48).


CONCLUSIONS
IgH(B) and IgK(B) primers of BIOMED-2 are suitable for the detection of clonal rearrangements of Ig gene using FFPE specimens of ocular adnexal lymphomas.
Wang
Yu-chuan
YC
Tianjin Eye Institute, Tianjin Eye Hospital, the Ophthalmic College of Tianjin Medical University, China.
Wang
Li-ming
LM
Hao
Peng
P
Ying
Ming
M
Han
Rui-fang
RF
Lin
Jin-yong
JY
D000199:Actins / Q000235:genetics*; D000328:Adult; D000368:Aged; D017931:DNA Primers; D005134:Eye Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D006801:Humans; D016393:Lymphoma, B-Cell / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D008224:Lymphoma, Follicular / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D010865:Pilot Projects; D055815:Young Adult
D000199:Actins; D017931:DNA Primers