title: RARgamma and TRbeta expressions are decreased in PBMC and SWAT of obese subjects in weight gain.
abstract: In order to evaluate the expression of nuclear receptors at the peripheral level in obese subjects, messenger RNA (mRNA) levels of different isoforms of retinoic acid receptor (RAR), triiodothyronine (TR), and peroxisome proliferator-activated receptor (PPAR) were determined and compared in peripheral mononuclear blood cells (PBMC) and subcutaneous white adipose tissue (SWAT). Twelve lean subjects and 68 obese subjects divided into weight gain (WG), weight-stable (WS), and weight loss (WL) groups were studied. Nuclear receptor mRNA levels were assessed in PBMC and SWAT using a quantitative real-time reverse transcription polymerase chain reaction method. mRNA levels of RARgamma were significantly lower in PBMC of obese subjects (WG -19%, WS -30%, and WL -24.7%) as in SWAT of WG (-50%). Lower mRNA levels of TRbeta were observed in PBMC and SWAT of WG (-50.7% and -28%, respectively) just as for TRalpha in PBMC of WG (-19%). In contrast, retinoid X receptors alpha (RXRalpha) and RARalpha mRNA levels were higher in PBMC of obese subjects (+53% and +54.5% in WG, +56% and +67% in WS, and +68% and +49.7% in WL, respectively), while expression of RXRalpha was lower in SWAT of WG (-24.5%). As for PPARgamma, its mRNA level was significantly higher in PBMC of WG subjects (+34%) while its expression was not modified in SWAT, contrary to the PPARgamma2 isoform which was significantly higher. These data show that in both adipose tissue and blood compartment of obese subjects, expressions of RARgamma and TRbeta were downregulated. Thus, we suggest that the expression in PBMC of obese subjects may constitute new cellular indicators of nuclear receptor retinoid and thyroid status.
authors.affiliation: Unité de Nutrition et Neurosciences (U2N), Université Bordeaux 1, Avenue des Facultés, 33405 Talence, France.
authors.forename: C
authors.initials: C
authors.lastname: Bairras
authors.forename: A
authors.initials: A
authors.lastname: Redonnet
authors.forename: H
authors.initials: H
authors.lastname: Dabadie
authors.forename: H
authors.initials: H
authors.lastname: Gin
authors.forename: C
authors.initials: C
authors.lastname: Atgie
authors.forename: V
authors.initials: V
authors.lastname: Pallet
authors.forename: P
authors.initials: P
authors.lastname: Higueret
authors.forename: C
authors.initials: C
authors.lastname: Noël-Suberville
mesh_terms: D000328:Adult; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008875:Middle Aged; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000235:genetics* / Q000378:metabolism; D050151:Subcutaneous Fat / Q000378:metabolism*; D014284:Triiodothyronine / Q000235:genetics* / Q000378:metabolism; D015430:Weight Gain / Q000235:genetics*; D000097768:Retinoic Acid Receptor gamma
chemical_list: D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D014284:Triiodothyronine; D000097768:Retinoic Acid Receptor gamma
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