title: [The pilot study on the correlation between human cytomegalovirus infection and the secretion of chemokines in the cultured human fibroblasts].
abstract: OBJECTIVE
  To investigate the effect of HCMV infection on the expression of interleukin-8 (IL-8) and regulated on activation, normal T expressed and secreted (RANTES) so as to explore the possible mechanism of the immune-pathological changes caused by HCMV infection.
  
  
  METHODS
  Expression of both IL-8 and RANTES mRNA was detected by RT-PCR. Secretion of IL-8 and RANTES protein was quantitated by enzyme linked immune-sororbant assay (ELISA).
  
  
  RESULTS
  After HCMV infection, the expression of IL-8 in HEL cells was found to be increased gradually concomitant with the prolonged infection time at both the transcriptional level and the protein level. By the time of 72 h.p.i, as compared with mock-infected cells, IL-8 mRNA expression was increased up to 12-fold and IL-8 protein up to 9-fold in HCMV-infected cells. The expression of RANTES mRNA was occurred at 8 h.p.i, with a maximum at 24 h.p.i, since then it remained relatively high level. The expression of RANTES protein peaked at 24 h.p.i, then dropped sharply and was almost undetectable by the time at 72 h.p.i.
  
  
  CONCLUSIONS
  HCMV infection of HEL cells may induce the transcription of IL-8 gene as well as the production of IL-8 orotein. HCMV may down-regulate extra-cellular production of RANTES protein.
authors.affiliation: Department of Gynecology and Obstetrics, Third hospital, Peking University, Beijing 100083, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shan
authors.initials: S
authors.lastname: Lu
authors.forename: Chun-yu
authors.initials: CY
authors.lastname: Zhang
authors.forename: Xue-ling
authors.initials: XL
authors.lastname: Song
mesh_terms: D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000276:immunology*; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D005260:Female; D005347:Fibroblasts / Q000276:immunology* / Q000821:virology; D015870:Gene Expression; D006801:Humans; D010865:Pilot Projects; D011247:Pregnancy
chemical_list: D018925:Chemokines