title: A Cys/Ser mutation of NDPK-A stabilizes its oligomerization state and enhances its activity.
abstract: Nucleoside diphosphate phosphate transferase A (NDPK-A) has been shown to play critical roles in the regulation of proliferation, differentiation, growth and apoptosis of cells. Our previous study suggested that the disulphide cross-linkage between cysteine 4 (C4) and cysteine 145 (C145) of NDPK-A might be a possible regulator of its activity. To confirm this hypothesis, the C145 residue of NDPK-A was mutated to serine, and the isomerization and biological activities of the mutant were investigated and compared with those of its wild-type counterpart. It was found the C145S mutation eliminated the intramolecular disulphide bond (DB) and prevented the formation of intermolecular DB, which was known to dissociate the hexameric NDPK-A into dimeric one. We also demonstrated that the C145S mutation didn't affect the autologous hexamerization of this protein, and the mutant had increased bioactivities including phosphate transferase and DNase. These findings support the hypothesis that the formation of DBs in NDPK-A is involved in the regulation of the oligomerization and bioactivity of this multiple function protein, and that C145 is a key residue in the regulation of NDPK-A. In addition, the C145S mutant that we have constructed might be an attractive candidate for use in applications that require NDPK-A.
authors.affiliation: Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, Guangdong, P. R. China 510632.
authors.forename: Yunru
authors.initials: Y
authors.lastname: Chen
authors.forename: Chuiwen
authors.initials: C
authors.lastname: Qian
authors.forename: Chaowan
authors.initials: C
authors.lastname: Guo
authors.forename: Feng
authors.initials: F
authors.lastname: Ge
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.forename: Shan
authors.initials: S
authors.lastname: Shen
authors.forename: Bing
authors.initials: B
authors.lastname: Lian
authors.forename: Kaio
authors.initials: K
authors.lastname: Kitazato
authors.forename: Yifei
authors.initials: Y
authors.lastname: Wang
authors.forename: Sheng
authors.initials: S
authors.lastname: Xiong
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D003545:Cysteine / Q000737:chemistry / Q000235:genetics; D003851:Deoxyribonucleases / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry; D016297:Mutagenesis, Site-Directed; D054778:NM23 Nucleoside Diphosphate Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D055503:Protein Multimerization / Q000235:genetics; D012694:Serine / Q000235:genetics
chemical_list: D004220:Disulfides; D054778:NM23 Nucleoside Diphosphate Kinases; D012694:Serine; D003851:Deoxyribonucleases; D003545:Cysteine