title: Self-assembly of the bacterial cytoskeleton-associated RNA helicase B protein into polymeric filamentous structures.
abstract: The Escherichia coli RNA degradosome proteins are organized into a helical cytoskeletal-like structure within the cell. Here we describe the ATP-dependent assembly of the RhlB component of the degradosome into polymeric filamentous structures in vitro, which suggests that extended polymers of RhlB are likely to comprise a basic core element of the degradosome cytoskeletal structures.
authors.affiliation: Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06032, USA. taghbalout@neuron.uchc.edu
authors.forename: Aziz
authors.initials: A
authors.lastname: Taghbalout
authors.forename: Qingfen
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D007150:Immunohistochemistry; D021381:Protein Transport
chemical_list: D029968:Escherichia coli Proteins; D000255:Adenosine Triphosphate; C502431:RhlB protein, E coli; D053487:DEAD-box RNA Helicases
references.citation: Annu Rev Microbiol. 2007;61:71-87
references.pmid: 17447862
references.citation: Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16776-81
references.pmid: 12482939
references.citation: Mol Microbiol. 1994 Nov;14(4):717-29
references.pmid: 7891559
references.citation: J Biol Chem. 2005 Jan 28;280(4):2628-35
references.pmid: 15548516
references.citation: EMBO J. 1999 May 4;18(9):2364-71
references.pmid: 10228151
references.citation: EMBO J. 1997 Sep 1;16(17):5455-63
references.pmid: 9312004
references.citation: Nature. 1996 May 9;381(6578):169-72
references.pmid: 8610017
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20209-14
references.pmid: 19088201
references.citation: Biochemistry. 2008 Jan 15;47(2):826-35
references.pmid: 18095710
references.citation: J Bacteriol. 1999 Feb;181(3):823-32
references.pmid: 9922245
references.citation: Adv Biophys. 1990;26:51-73
references.pmid: 2082729
references.citation: Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1465-70
references.pmid: 11171974
references.citation: Proc Natl Acad Sci U S A. 2002 May 14;99(10):6761-6
references.pmid: 11983867
references.citation: Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1667-72
references.pmid: 17242352
references.citation: J Biol Chem. 2008 May 16;283(20):13850-5
references.pmid: 18337249
references.citation: J Biol Chem. 2002 Oct 25;277(43):41157-62
references.pmid: 12181321
references.citation: Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16590-5
references.pmid: 16275923
references.citation: Cell. 1994 Mar 11;76(5):889-900
references.pmid: 7510217
references.citation: Nature. 2001 Sep 6;413(6851):39-44
references.pmid: 11544518
references.citation: J Biol Chem. 2004 Nov 19;279(47):48821-9
references.pmid: 15328358
references.citation: Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):3865-9
references.pmid: 8632981
references.citation: Mol Microbiol. 2008 Nov;70(4):780-2
references.pmid: 18990179
references.citation: New Biol. 1991 Sep;3(9):886-95
references.pmid: 1931833