[Effects of azadirachtin on rice plant volatiles induced by Nilaparvata lugens].
With the method of solid phase microextraction (SPME), a total of twenty-five volatiles were collected from rice plants induced by Nilaparvata lugens, and after applying azadirachtin fourteen of them were qualitatively identified by gas chromatography coupled by mass spectrometry (GC-MS), mainly of nine kinds of sesquiterpenes. Comparing with healthy rice plants, the plants attacked by N. lugens had more kinds of volatiles, including limonene, linalool, methyl salicylate, unknown 6, unknown 7, zingiberene, nerolidol, and hexadecane. Applying azadirachtin did not result in the production of new kind volatiles, but affected the relative concentrations of the volatiles induced by N. lugens. The proportions of limonene, linalool, methyl salicylate, unknown 6, zingiberene, and hexadecane changed obviously with the concentration of applied azadirachtin, while those of methyl salicylate, unknown 6, unknown 7, zingiberene, and nerolidol changed significantly with the days after azadirachtin application. Azadirachtin concentration, rice variety, and N. lugens density had significant interactions on the relative concentrations of all test N. lugens-induced volatiles.
Lu
Hai-Yan
HY
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu, China. 54521955@qq.com
Liu
Fang
F
Zhu
Shu-De
SD
Zhang
Qing
Q
D000818:Animals; D002630:Chemotactic Factors / Q000032:analysis / Q000378:metabolism; D006430:Hemiptera / Q000502:physiology*; D006790:Host-Parasite Interactions; D036701:Limonins / Q000494:pharmacology*; D012275:Oryza / Q000187:drug effects* / Q000378:metabolism* / Q000469:parasitology; D012717:Sesquiterpenes / Q000032:analysis / Q000378:metabolism; D014835:Volatilization
D002630:Chemotactic Factors; D036701:Limonins; D012717:Sesquiterpenes; C010329:azadirachtin