Identification of two small heat shock proteins with different response profile to cadmium and pathogen stresses in Venerupis philippinarum.
Small heat shock proteins (sHSPs) encompass a widespread and diverse class of proteins with molecular chaperone activity. In the present study, two sHSP isoforms (VpsHSP-1 and VpsHSP-2) were cloned from Venerupis philippinarum haemocytes by Rapid Amplification of cDNA Ends (RACE) approaches. The expression profiles of these two genes under Vibrio anguillarum challenge and cadmium exposure were investigated by quantitative real-time reverse transcriptase polymerase chain reaction. The bacterial challenge could significantly up-regulate the mRNA expression of both VpsHSP-1 and VpsHSP-2, with the increase of VpsHSP-2 expression occurred earlier than that of VpsHSP-1. During the cadmium exposure experiment, the expression level of both VpsHSP-1 and VpsHSP-2 decreased significantly with larger amplitude in VpsHSP-2. As time progressed, the expression levels of both genes were up-regulated with more increment in the low-chemical exposure groups. The differences in the response to pathogen stimulation and cadmium exposure indicated that there were functional diversity between the two structurally different molecules, VpsHSP-1 and VpsHSP-2, and they probably played distinct roles in mediating the environmental stress and immune responses in calm.
Li
Chenghua
C
Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 17 Chunhui Road, Laishan District, 264003, Yantai, People's Republic of China.
Wang
Lingling
L
Ning
Xuanxuan
X
Chen
Aiqin
A
Zhang
Linbao
L
Qin
Song
S
Wu
Huifeng
H
Zhao
Jianmin
J
D000595:Amino Acid Sequence; D000818:Animals; D049872:Bivalvia / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D002104:Cadmium / Q000633:toxicity*; D020869:Gene Expression Profiling; D050888:Heat-Shock Proteins, Small / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D013997:Time Factors; D014733:Vibrio / Q000472:pathogenicity*
D050888:Heat-Shock Proteins, Small; D002104:Cadmium
Breast Cancer Res Treat. 2010 Nov;124(2):307-15
20087649
Trends Immunol. 2002 Mar;23(3):130-5
11864840
Cell Mol Life Sci. 2002 Oct;59(10):1649-57
12475175
Brief Bioinform. 2004 Jun;5(2):150-63
15260895
Trends Immunol. 2004 Aug;25(8):427-33
15275642
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13504-9
12368478
Comp Biochem Physiol B Biochem Mol Biol. 2007 Aug;147(4):704-15
17553718
Cell Stress Chaperones. 2010 Jul;15(4):351-63
19953352
Curr Opin Immunol. 2002 Feb;14(1):45-51
11790532
Cell Mol Immunol. 2004 Aug;1(4):274-9
16225770
Hematology. 2005 Jun;10(3):225-35
16019471
Nat Rev Immunol. 2002 Mar;2(3):185-94
11913069
Mol Biol Rep. 2010 Apr;37(4):1763-71
19585273
Fish Shellfish Immunol. 2008 Sep;25(3):250-7
18603001
Fish Shellfish Immunol. 2010 Mar;28(3):499-503
19948222
Lancet. 2003 Aug 9;362(9382):469-76
12927437
Annu Rev Genet. 1988;22:631-77
2853609
Comp Biochem Physiol A Mol Integr Physiol. 2009 Jan;152(1):91-9
18834946
J Biol Chem. 2000 Oct 6;275(40):31204-10
10896659
Fish Shellfish Immunol. 2006 Oct;21(4):335-45
16530426
Bull Environ Contam Toxicol. 2000 Jun;64(6):852-8
10856343
Hum Exp Toxicol. 1996 Apr;15(4):279-85
8845215
Am J Physiol Cell Physiol. 2004 Apr;286(4):C739-44
15001423
Immunopharmacology. 2000 Jul 25;48(3):299-302
10960671
J Biol Chem. 1996 Jul 12;271(28):16510-4
8663291