title: Low-level laser therapy (LLLT) attenuates RhoA mRNA expression in the rat bronchi smooth muscle exposed to tumor necrosis factor-alpha.
abstract: Low-level laser therapy (LLLT) has been found to produce anti-inflammatory effects in a variety of disorders. Bronchial smooth muscle (BSM) hyperreactivity is associated with increased Ca+2 sensitivity and increased RhoA mRNA expression. In the current study, we investigated if LLLT could reduce BSM contraction force and RhoA mRNA expression in tumor necrosis factor-alpha (TNF-alpha)-induced BSM hyperreactivity. In the study, 112 male Wistar rats were divided randomly into 16 groups, and BSM was harvested and suspended in TNF-alpha baths for 6 and 24 h, respectively. Irradiation with LLLT was performed with a wavelength of 660 nm for 42 s with a dose of 1.3 J/cm2. This LLLT dose was administered once in the 6-h group and twice in the 24-h group. LLLT significantly decreased contraction force in BSM at 6 h (TNF-alpha + LLLT: 11.65+/-1.10 g/100 mg of tissue) (F=3115) and at 24 h (TNF-alpha+ LLLT: 14.15+/-1.1 g/100 mg of tissue) (F=3245, p<0.05) after TNF-alpha, respectively, when compared to vehicle-bathed groups (control). LLLT also significantly decreased the expression of RhoA mRNA in BSM segments at 6 h (1.22+/-0.20) (F=2820, p<0.05) and 24 h (2.13+/-0.20) (F=3324, p<0.05) when compared to BSM segments incubated with TNF-alpha without LLLT irradiation. We conclude that LLLT administered with this protocol, reduces RhoA mRNA expression and BSM contraction force in TNF-alpha-induced BSM hyperreactivity.
authors.affiliation: Instituto de Pesquisa e Desenvolvimento, IP&D, Av. Shishima Hifumi, 2911, São José dos Campos, CEP: 12244-000 São Paulo, SP, Brazil.
authors.forename: Flávia Mafra
authors.initials: FM
authors.lastname: de Lima
authors.forename: Jan M
authors.initials: JM
authors.lastname: Bjordal
authors.forename: Regiane
authors.initials: R
authors.lastname: Albertini
authors.forename: Fábio V
authors.initials: FV
authors.lastname: Santos
authors.forename: Flavio
authors.initials: F
authors.lastname: Aimbire
mesh_terms: D000577:Amides / Q000494:pharmacology; D000818:Animals; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D016535:Bronchial Hyperreactivity / Q000139:chemically induced / Q000235:genetics / Q000532:radiotherapy; D020013:Calcium Signaling / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D066298:In Vitro Techniques; D028022:Low-Level Light Therapy; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D011725:Pyridines / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D020742:rhoA GTP-Binding Protein / Q000235:genetics*
chemical_list: D000577:Amides; D011725:Pyridines; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C108830:Y 27632; D020742:rhoA GTP-Binding Protein
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