Derivation and long-term culture of human parthenogenetic embryonic stem cells using human foreskin feeders.
OBJECTIVE
Feeder cells from animals raise considerable concern for contamination because they are directly in contact with embryonic stem cells.


METHODS
To address this issue we collected discarded foreskin tissue and prepared a fibroblast cell line. We transferred one parthenogenetic blastocyst on to these feeder cells, and later observed outgrowth. By this approach, we were able to derive a human parthenogenetic embryonic stem cell line successfully.


RESULTS
The embryonic stem cells had normal morphology, expressed all expected cell surface markers, could differentiate to embryonic bodies upon culture in vitro, and differentiated further to derivatives of all three germ layers.


CONCLUSIONS
This study indicates that homologous human fibroblasts can be used as feeder cells to support not only the propagation, but also the derivation of ES cells, and this should facilitate studies of therapeutic cloning for research and clinical applications.
Lu
Zhenyu
Z
Center for Reproductive Medicine, Tianjin Central Hospital for Obstetrics and Gynecology, Tianjin 300052, People's Republic of China.
Zhu
Wanwan
W
Yu
Yang
Y
Jin
Dan
D
Guan
Yunqian
Y
Yao
Ruqiang
R
Zhang
Yu Alex
YA
Zhang
Yunshan
Y
Zhou
Qi
Q
D018929:Cell Culture Techniques; D002460:Cell Line; D018920:Coculture Techniques; D053595:Embryonic Stem Cells; D005347:Fibroblasts / Q000166:cytology; D052816:Foreskin / Q000166:cytology*; D006801:Humans; D007621:Karyotyping; D008297:Male
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