Spatial and temporal changes of osteopontin in oxygen-glucose-deprived hippocampal slice cultures.
We have examined the temporal changes and cellular localization of osteopontin (OPN) mRNA and protein in organotypic hippocampal slice cultures subjected to ischemia-like oxygen-glucose deprivation (OGD). The sequential induction pattern response consisted of neuronal and microglial OPN upregulation, followed by a later extended phase of expression in reactive astrocytes. OPN immunoreactivity after OGD matched the mRNA induction patterns. Activated microglia revealed OPN staining in focal deposits, whereas neurons and reactive astrocytes showed perinuclear staining with a punctate cytosolic pattern of OPN, typical of secreted proteins. These data demonstrated that the temporal and cellular patterns of OPN induction in reactive glial cells in this in vitro model closely correlated with that in the in vivo model, suggesting that OPN has a multifunctional role in the pathogenesis of ischemic injury.
Lee
Ji-Yeon
JY
Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Choi
Jeong-Sun
JS
Choi
Jae-Youn
JY
Shin
Yoo-Jin
YJ
Yun
Hou
H
Cha
Jung-Ho
JH
Chun
Myung-Hoon
MH
Lee
Mun-Yong
MY
D000818:Animals; D000831:Animals, Newborn; D002135:Calcium-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000172:deficiency*; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D000860:Hypoxia / Q000473:pathology*; D008840:Microfilament Proteins; D009474:Neurons / Q000378:metabolism; D009924:Organ Culture Techniques; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; D053495:Osteopontin; D010751:Phosphopyruvate Hydratase; D005947:Glucose