title: Rad21 is required for centrosome integrity in human cells independently of its role in chromosome cohesion.
abstract: Classically, chromosomal functions in DNA repair and sister chromatid association have been assigned to the cohesin proteins. More recent studies have provided evidence that cohesins also localize to the centrosomes, which organize the bipolar spindle during mitosis. Depletion of cohesin proteins is associated with multi-polar mitosis in which spindle pole integrity is compromised. However, the spindle pole defects after cohesin depletion could be an indirect consequence of a chromosomal cohesion defect which might impact centrosome integrity via alterations to the spindle microtubule network. Here we show that the cohesin Rad21 is required for centrosome integrity independently of its role as a chromosomal cohesin. Thus, Rad21 may promote accurate chromosome transmission not only by virtue of its function as a chromosomal cohesin, but also because it is required for centrosome function.
authors.affiliation: Department of Genetics, Cell Biology & Development, University of Minnesota Medical School, Minneapolis, MN, USA.
authors.forename: Nicole A
authors.initials: NA
authors.lastname: Beauchene
authors.forename: Laura A
authors.initials: LA
authors.lastname: Díaz-Martínez
authors.forename: Katherine
authors.initials: K
authors.lastname: Furniss
authors.forename: Wei-Shan
authors.initials: WS
authors.lastname: Hsu
authors.forename: Hung-Ji
authors.initials: HJ
authors.lastname: Tsai
authors.forename: Chris
authors.initials: C
authors.lastname: Chamberlain
authors.forename: Pedro
authors.initials: P
authors.lastname: Esponda
authors.forename: Juan F
authors.initials: JF
authors.lastname: Giménez-Abián
authors.forename: Duncan J
authors.initials: DJ
authors.lastname: Clarke
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism; D018385:Centrosome / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D020090:Chromosome Segregation; D004268:DNA-Binding Proteins; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D008938:Mitosis; D009687:Nuclear Proteins / Q000378:metabolism* / Q000502:physiology; D010750:Phosphoproteins / Q000378:metabolism* / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D000097722:Cohesins; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D000097722:Cohesins; D000097869:Polo-Like Kinase 1; C429022:RAD21 protein, human