Inhibition of isolated human myometrium contractility by minoxidil and reversal by glibenclamide.
This study investigated the ability of the antihypertensive drug minoxidil to inhibit potassium chloride (KCl)-induced contractility of the isolated human myometrium. Twelve strips of myometrium obtained from 12 patients who underwent hysterectomy were triggered to contract with 55 mM KCl before and after incubation with 3 concentrations (1, 3 and 10 microM) of minoxidil. The percent inhibition by minoxidil on the extent of contraction, and the area under the contractile curve of KCl-induced contraction of the myometrial strips was determined. Furthermore, the effect of 10 microM glibenclamide on the inhibition generated by 3 microM minoxidil on KCl-induced contractility was studied. It was found that minoxidil produced a concentration-dependent inhibition of KCl-induced contractility of the myometrium and that glibenclamide reversed this inhibitory effect. These results suggest that the inhibitory effect of minoxidil on isolated human myometrium contractility may prove useful in clinical conditions requiring relaxation of the myometrium.
Prabhakaran
S S
SS
Department of Pharmacology and Clinical Pharmacology, Christian Medical College, Vellore, India.
Dhanasekar
K R
KR
Thomas
E
E
Jose
R
R
Peedicayil
J
J
Samuel
P
P
D000328:Adult; D019540:Area Under Curve; D004305:Dose-Response Relationship, Drug; D005260:Female; D005905:Glyburide / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D008914:Minoxidil / Q000008:administration & dosage / Q000494:pharmacology*; D009215:Myometrium / Q000187:drug effects* / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D014590:Uterine Contraction / Q000187:drug effects*; D014665:Vasodilator Agents / Q000008:administration & dosage / Q000494:pharmacology; D055815:Young Adult
D014665:Vasodilator Agents; D008914:Minoxidil; D011189:Potassium Chloride; D005905:Glyburide