[Genetic analysis of HAV strains isolated from patients with acute hepatitis in Hebei Shijiazhuang of China].
OBJECTIVE
To characterize the hepatitis A virus (HAV) wild type strains circulating in Hebei Shijiazhuang of China during 2005-2007, to provide the bases for further investigation of the sources of HAV infection.


METHODS
The VP1/P2A junction regions were detected by RT-PCR from HAV IgM positives serum samples during 2005 and 2007, the 34 RT-PCR positive samples were sequenced and subjected to phylogenetic analysis by Neighbor Joining (NJ) method.


RESULTS
All the detected HAV strains were identified as sub-genotype I A, the homology of nucleotide sequence in the VP1-2A imation region ranged from 95%-100%, the amino acid sequences of HAV strains almost had no difference.


CONCLUSIONS
There are different HAV strains existing in Hebei Shijiazhuang of China, same HAV strain may exist in different areas; or in one area, identical or different HAV strains may be detected. This work provides the bases for further investigation of the sources of HAV infection and also for effectively control measures to prevent the spread of the disease.
Dai
Er-hei
EH
Department of Laboratory Medicine, the Fifth Hospital of Shijiazhuang, Hebei 050021, China.
Guo
Zi-jie
ZJ
Lu
Jian-hua
JH
Lu
Yu-zhen
YZ
Hou
Jun-liang
JL
Cao
Zhi-chen
ZC
Meng
Qing-ling
QL
Cao
Jing-yuan
JY
Bi
Sheng-li
SL
D000208:Acute Disease; D000293:Adolescent; D002648:Child; D002681:China; D005260:Female; D006506:Hepatitis A / Q000821:virology*; D017957:Hepatitis A Virus, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D015678:Viral Structural Proteins / Q000235:genetics; D055815:Young Adult
C096539:VP1 protein, hepatitis A virus; D015678:Viral Structural Proteins