title: Determination of triazines in honey by dispersive liquid-liquid microextraction high-performance liquid chromatography.
abstract: Dispersive liquid-liquid microextraction (DLLME) high-performance liquid chromatography (HPLC) was developed for extraction and determination of triazines from honey. A room temperature ionic liquid, 1-hexyl-3-methylimidazolium hexafluorophosphate [C(6)MIM][PF(6.)], was used as extraction solvent and Triton X 114 was used as dispersant. A mixture of 175 microL [C(6)MIM][PF(6)] and 50 microL 10% Triton X 114 was rapidly injected into the 20 mL honey sample by syringe. After extraction, phase separation was performed by centrifugation and the sedimented phase was analyzed by HPLC. Some experimental parameters, such as type and volume of extraction solvent, concentration of dispersant, pH value of sample solution, salt concentration and extraction time were investigated and optimized. The detection limits for chlortoluron, prometon, propazine, linuron and prebane are 6.92, 5.84, 8.55, 8.59 and 5.31 microg kg(-1), respectively. The main advantages of the proposed method are simplicity of operation, low cost, high enrichment factor and extraction solvent volume at microliter level. Honey samples were analyzed by the proposed method and obtained results indicated that the proposed method provides acceptable recoveries and precisions.
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Jingyan
authors.initials: J
authors.lastname: You
authors.forename: Ruibing
authors.initials: R
authors.lastname: Ren
authors.forename: Yao
authors.initials: Y
authors.lastname: Xiao
authors.forename: Shiqian
authors.initials: S
authors.lastname: Gao
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
authors.forename: Aimin
authors.initials: A
authors.lastname: Yu
mesh_terms: D001881:Borates / Q000737:chemistry; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006722:Honey / Q000032:analysis*; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry; D057230:Limit of Detection; D014227:Triazines / Q000032:analysis*
chemical_list: C528767:1-hexyl-3-methylimidazolium; D001881:Borates; D007093:Imidazoles; D052578:Ionic Liquids; D014227:Triazines