DNA bending and looping in the transcriptional control of bacteriophage phi29.
Recent studies on the regulation of phage phi29 gene expression reveal new ways to accomplish the processes required for the orderly gene expression in prokaryotic systems. These studies revealed a novel DNA-binding domain in the phage main transcriptional regulator and the nature and dynamics of the multimeric DNA-protein complex responsible for the switch from early to late gene expression. This review describes the features of the regulatory mechanism that leads to the simultaneous activation and repression of transcription, and discusses it in the context of the role of the topological modification of the DNA carried out by two phage-encoded proteins working synergistically with the DNA.
Camacho
Ana
A
Centro de Biología Molecular 'Severo Ochoa' (CSIC-UAM), Instituto de Biología Molecular 'Eladio Viñuela' (CSIC), Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain.
Salas
Margarita
M
D017103:Bacillus Phages / Q000235:genetics*; D001412:Bacillus subtilis / Q000821:virology; D001483:Base Sequence; D004279:DNA, Viral / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D016415:Sequence Alignment; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D014764:Viral Proteins; C053987:p4 protein, Bacteriophage phi 29; C080198:viral protein p6