[Evaluation of the phagocytic activity of human peripheral blood neutrophils, by employing recombinant luminescent bacteria].
The authors show it possible to evaluate the phagocytic activity of human peripheral blood neutrophils from their impact on the fluorescence intensity of recombinant luminescent bacteria. They have established the preferred use of recombinant luminescent Escherichia coli bacteria with cloned luxCDABE genes OF Photobacterium leiognathii as phagocyted objects and substantiated the optimal method of their opsonization by normal human immunoglobulin. The variants of separate consideration of the luminal-dependent chemiluminescence of phagocytes and the bioluminescence of bacterial target in the phagocytic system, which are based on the study of two tests of different component composition or one test at two wavelengths of less than 420 nm for the assessment of chemiluminescence and more than 540 nm for the assessment of bioluminescence.
Deriabin
D G
DG
Karimov
I F
IF
D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007136:Immunoglobulins; D008163:Luminescent Measurements; D009504:Neutrophils / Q000502:physiology*; D009895:Opsonin Proteins; D010587:Phagocytosis; D010776:Photobacterium / Q000235:genetics; D020637:Photorhabdus / Q000235:genetics
D007136:Immunoglobulins; D009895:Opsonin Proteins