Genetic variation among Staphylococcus aureus strains from bovine milk and their relevance to methicillin-resistant isolates from humans.
In genetic analysis of bovine Staphylococcus aureus isolates that are recognized as an important pathogenic bacterium in bovine mastitis, multilocus sequence typing (MLST) showed strong correlation to the results of pulsed-field gel electrophoresis, coa PCR-restriction fragment length polymorphism (RFLP), spa typing, and the coagulase serotyping method. According to MLST results, strains derived from sequence type 97 (ST97) and ST705 were suggested as not only dominant bovine S. aureus lineages in Japan but also pandemic bovine S. aureus lineages. Although both lineages seem to be distantly related to each other by phylogenetic analysis, both had common characteristics, i.e., lukM/lukF'-PV and coagulase serotype VI. These characteristics were very rare among minor bovine strains and human strains and may contribute to the host specificity of these lineages. Four methicillin-resistant S. aureus (MRSA) isolates were first confirmed from bovine milk in Japan; these isolates showed geno- and serotypes that were identical or similar to those of human MRSA isolates in Japan (ST5, staphylococcal cassette chromosome mec type II [SCCmec II], Spa type t002 or t375, and coagulase serotype II, and ST89, SCCmec IIIa, Spa type t5266, and coagulase serotype I). ST5 and ST89 are uncommon among bovine isolates in the world, whereas these STs are common among human MRSA isolates in Japan.
Hata
Eiji
E
Research Team for Bacterial/Parasitic Diseases, Hokkaido Research Station, National Institute of Animal Health, 4 Hitsujigaoka, Toyohira-ku, Sapporo, Hokkaido 062-0045, Japan. ehata@affrc.go.jp
Katsuda
Ken
K
Kobayashi
Hideki
H
Uchida
Ikuo
I
Tanaka
Kiyoshi
K
Eguchi
Masashi
M
D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D016000:Cluster Analysis; D003030:Coagulase / Q000145:classification; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007564:Japan; D008414:Mastitis, Bovine / Q000382:microbiology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008892:Milk / Q000382:microbiology*; D017720:Molecular Epidemiology; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013203:Staphylococcal Infections / Q000382:microbiology* / Q000662:veterinary*
D003030:Coagulase; D004269:DNA, Bacterial
Microb Drug Resist. 2005 Fall;11(3):279-86
16201932
PLoS One. 2008 Aug 27;3(8):e3074
18728754
J Clin Microbiol. 2000 Mar;38(3):1008-15
10698988
Microbiol Immunol. 2008 Jul;52(7):334-48
18667032
Appl Environ Microbiol. 2003 Nov;69(11):6489-94
14602604
J Clin Microbiol. 2002 May;40(5):1821-3
11980967
J Clin Microbiol. 2005 Sep;43(9):4731-6
16145134
J Clin Microbiol. 2005 Jan;43(1):421-6
15635004
J Clin Microbiol. 2008 Sep;46(9):3147-51
18650364
J Clin Microbiol. 2003 Aug;41(8):3806-15
12904393
J Clin Microbiol. 1998 Apr;36(4):1083-9
9542942
Vet Q. 2002 Dec;24(4):181-98
12540135
Vet Microbiol. 2001 Sep 20;82(2):187-99
11423209
J Clin Microbiol. 2003 Dec;41(12):5442-8
14662923
J Dairy Sci. 2008 Feb;91(2):564-9
18218742
J Antimicrob Chemother. 2005 Oct;56(4):624-32
16126781
J Clin Microbiol. 1991 Dec;29(12):2690-5
1757534
Appl Environ Microbiol. 2005 Dec;71(12):8352-61
16332822
J Vet Med Sci. 2006 Feb;68(2):165-70
16520540
J Bacteriol. 2004 Mar;186(5):1518-30
14973027
PLoS One. 2007 Oct 31;2(10):e1120
17971880
J Clin Microbiol. 2005 Sep;43(9):4737-43
16145135
Vet Microbiol. 2009 May 12;136(3-4):277-84
19101101
Infect Immun. 2002 Feb;70(2):631-41
11796592
Emerg Infect Dis. 2003 Aug;9(8):978-84
12967497
Vet Microbiol. 2005 Sep 30;110(1-2):97-103
16112825
J Clin Microbiol. 1998 Mar;36(3):618-23
9508283
J Clin Microbiol. 2005 Aug;43(8):3979-84
16081939
Antimicrob Agents Chemother. 2002 Jul;46(7):2155-61
12069968
J Clin Microbiol. 1995 Mar;33(3):551-5
7751356
J Med Microbiol. 2007 Nov;56(Pt 11):1505-1511
17965353
J Clin Microbiol. 2006 Jan;44(1):271-3
16390988
J Antimicrob Chemother. 2007 Jul;60(1):42-8
17468509
J Clin Microbiol. 2005 Oct;43(10):5026-33
16207957
J Clin Microbiol. 1995 Sep;33(9):2233-9
7494007
Can Vet J. 1968 May;9(5):107-15
5649956
Appl Environ Microbiol. 2007 Jun;73(12):3845-9
17449696
Res Vet Sci. 1975 Jul;19(1):23-7
125447
Microbes Infect. 2006 Jul;8(8):2068-74
16782383