title: A model of isolated, autologously hemoperfused porcine slaughterhouse lungs.
abstract: BACKGROUND
  Models of isolated and perfused lungs study pathophysiological phenomena of the airways, but are limited by restricted resemblance to the human situation, non-physiological perfusates or the need for the use of high numbers of laboratory animals. The present model was established to address these difficulties.
  
  
  OBJECTIVE
  Aim of the current study was the establishment of an animal model that uses slaughterhouse animals and closely resembles physiological conditions found in humans.
  
  
  METHODS
  We used a model of hemoperfused isolated porcine slaughterhouse lungs using autologous blood, metabolically controlled via a dialysis system. Over a period of 135 minutes positive inspiratory pressure, pulmonary arterial pressure, pulmonary vein oxygen partial pressure and lung weight were assessed.
  
  
  RESULTS
  Stable organ function was maintained over 135 minutes with an amount of 2,500-3,000 ml perfusate without fall in pulmonary arterial pressure. During the time the positive inspiratory pressure and lung weight increased, while pulmonary vein oxygen partial pressure decreased.
  
  
  CONCLUSIONS
  The present model of isolated hemoperfused slaughterhouse lungs displays a useful new and economic approach to evaluate pulmonary function and toxicity of different substances on an organ level. As a major economic advantage in comparison to models using laboratory animals, the current model might be run using blood and organs obtained from slaughterhouse animals.
authors.affiliation: Dept. of Radiology, Westkuestenkliniken Heide, Germany. paipman@web.de
authors.forename: Peter C
authors.initials: PC
authors.lastname: Kamusella
authors.forename: Christian
authors.initials: C
authors.lastname: Wissgott
authors.forename: Christian
authors.initials: C
authors.lastname: Grosse-Siestrup
authors.forename: Sven
authors.initials: S
authors.lastname: Dittrich
authors.forename: Olaf
authors.initials: O
authors.lastname: Hegemann
authors.forename: Dimitri
authors.initials: D
authors.lastname: Koios
authors.forename: Juliane
authors.initials: J
authors.lastname: von Massenbach
authors.forename: Michael
authors.initials: M
authors.lastname: Meissler
authors.forename: Volker
authors.initials: V
authors.lastname: Unger
authors.forename: David
authors.initials: D
authors.lastname: Quarcoo
authors.forename: David A
authors.initials: DA
authors.lastname: Groneberg
mesh_terms: D000003:Abattoirs; D000818:Animals; D008168:Lung / Q000033:anatomy & histology / Q000098:blood supply* / Q000502:physiology*; D009924:Organ Culture Techniques; D009929:Organ Size; D010600:Pharmacology / Q000379:methods; D012129:Respiratory Function Tests / Q000379:methods*; D013552:Swine