title: PdhS, an old-pole-localized histidine kinase, recruits the fumarase FumC in Brucella abortus.
abstract: The bacterial pathogen Brucella abortus was recently demonstrated to recruit the essential cytoplasmic histidine kinase PdhS to its old pole. Here, we report identification of the fumarase FumC as a specific partner for the N-terminal "sensing" domain of PdhS, using an ORFeome-based yeast two-hybrid screen. We observed that FumC and PdhS colocalize at the old pole of B. abortus, while the other fumarase FumA is not polarly localized. FumC is not required for PdhS localization, and polar FumC localization is not FumA dependent. FumC homologs are not polarly localized in Sinorhizobium meliloti and Caulobacter crescentus, suggesting that polar recruitment of FumC by PdhS is evolutionarily recent.
authors.affiliation: Molecular Biology Research Unit (URBM), University of Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium.
authors.forename: Johann
authors.initials: J
authors.lastname: Mignolet
authors.forename: Charles
authors.initials: C
authors.lastname: Van der Henst
authors.forename: Cécile
authors.initials: C
authors.lastname: Nicolas
authors.forename: Michaël
authors.initials: M
authors.lastname: Deghelt
authors.forename: Delphine
authors.initials: D
authors.lastname: Dotreppe
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Letesson
authors.forename: Xavier
authors.initials: X
authors.lastname: De Bolle
mesh_terms: D002003:Brucella abortus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D005649:Fumarate Hydratase / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D000071677:Histidine Kinase; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D011494:Protein Kinases; D000071677:Histidine Kinase; D005649:Fumarate Hydratase
references.citation: Trends Microbiol. 2004 Aug;12(8):361-5
references.pmid: 15276611
references.citation: Biochem J. 1986 Jul 15;237(2):547-57
references.pmid: 3541901
references.citation: EMBO J. 2007 Mar 7;26(5):1444-55
references.pmid: 17304218
references.citation: Curr Opin Microbiol. 2004 Feb;7(1):93-7
references.pmid: 15036147
references.citation: J Bacteriol. 2006 Jun;188(12):4508-21
references.pmid: 16740957
references.citation: Cell Microbiol. 2006 Nov;8(11):1791-802
references.pmid: 16803581
references.citation: Biochim Biophys Acta. 1988 Apr 28;954(1):14-26
references.pmid: 3282546
references.citation: Genome Res. 2004 Oct;14(10B):2201-6
references.pmid: 15489343
references.citation: J Bacteriol. 2007 May;189(9):3591-602
references.pmid: 17237174