title: Utilization of a novel autologous killed tri-vaccine (serogroups B [Typhimurium], C [Mbandaka] and E [Orion]) for Salmonella control in commercial poultry breeders.
abstract: An autologous killed trivalent vaccine (3x10(8) colony-forming units [CFU]), based on three Salmonella serovars (Typhimurium - serogroup B, Mbandaka - serogroup C, and Orion - serogroup E) prevalent in the flocks of Australian poultry companies, was developed using Salenvac techniques. At 20 weeks, hens vaccinated at 12 and 17 weeks as well as non-vaccinated hens were challenged (250 microl of 10(7) CFU) with autologous and heterologous serovars belonging to serogroup B (Typhimurium and Agona), serogroup C (Mbandaka and Infantis) and serogroup E (Orion and Zanzibar). Overall, vaccination resulted in a significant difference in carriage of Salmonella between non-vaccinated and vaccinated commercial Cobb hens (P <0.05) for serogroups B and C. However, due to low colonization rates in the non-vaccinated birds, no significant difference (P>0.05) could be determined for serogroup E. All vaccinated flocks produced a significant antibody response (P<0.001) to the S. Typhimurium vaccine strain, measured using a S. Typhimurium enzyme-linked immunosorbent assay (Guildhay), which peaked at 20 weeks of age, with 39% of the hens positive. Maternal antibodies were detected in 16% of the yolks from eggs produced by these flocks. There was a significant difference after challenge with Salmonella (P <0.05) among 1-day-old chicks from vaccinated versus non-vaccinated parents, when challenged using 10(4) CFU but not when challenged with 10(8) CFU. The success of this trial resulted in the incorporation of this vaccine into a Salmonella control system in commercial broiler breeder production.
authors.affiliation: Birling Avian Laboratories, Sydney, Australia. tony_pavic@baiada.com.au
authors.forename: Anthony
authors.initials: A
authors.lastname: Pavic
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Groves
authors.forename: Julian M
authors.initials: JM
authors.lastname: Cox
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000907:Antibodies, Bacterial; D001315:Australia; D002645:Chickens; D004530:Egg Yolk / Q000276:immunology*; D005260:Female; D007109:Immunity; D007115:Immunization Schedule; D008297:Male; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D022562:Salmonella Vaccines / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D022562:Salmonella Vaccines; D015164:Vaccines, Inactivated