Expression, purification and preliminary X-ray diffraction analysis of the catalytic module of a beta-agarase from the flavobacterium Zobellia galactanivorans.
Marine bacteria secrete specific glycoside hydrolases such as agarases to access polysaccharides from algal cell walls as a carbon and energy source. In an attempt to identify agarases with variable degradation patterns, a novel family GH16 beta-agarase from the marine bacterium Zobellia galactanivorans was expressed, purified and crystallized. The purified enzyme crystallized in two distinct forms that were grown by the hanging-drop vapour-diffusion method using polyethylene glycol as a precipitant. Hexagonal crystals belonging to space group P3(1)21 diffracted to 2.2 A resolution, whereas orthorhombic crystals belonging to space group P2(1)2(1)2(1) diffracted to 1.5 A resolution.
Hehemann
Jan Hendrik
JH
Université Pierre et Marie Curie-Paris 6, Unité Mixte de Recherche 7139 Marine Plants and Biomolecules, Station Biologique, F-29682 Roscoff CEDEX, Bretagne, France.
Michel
Gurvan
G
Barbeyron
Tristan
T
Czjzek
Mirjam
M
D000595:Amino Acid Sequence; D055162:Biocatalysis; D018360:Crystallography, X-Ray; D041965:Flavobacteriaceae / Q000201:enzymology*; D015870:Gene Expression; D006026:Glycoside Hydrolases / Q000737:chemistry*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D006026:Glycoside Hydrolases; C019624:agarase
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