title: Sprouty1 is a critical regulatory switch of mesenchymal stem cell lineage allocation.
abstract: Development of bone and adipose tissue are linked processes arising from a common progenitor cell, but having an inverse relationship in disease conditions such as osteoporosis. Cellular differentiation of both tissues relies on growth factor cues, and we focus this study on Sprouty1 (Spry1), an inhibitor of growth factor signaling. We tested whether Spry1 can modify the development of fat cells through its activity in regulating growth factors known to be important for adipogenesis. We utilized conditional expression and genetic-null mouse models of Spry1 in adipocytes using the fatty acid binding promoter (aP2). Conditional deletion of Spry1 results in 10% increased body fat and decreased bone mass. This phenotype was rescued on Spry1 expression, which results in decreased body fat and increased bone mass. Ex vivo bone marrow experiments indicate Spry1 in bone marrow and adipose progenitor cells favors differentiation of osteoblasts at the expense of adipocytes by suppressing CEBP-beta and PPARgamma while up regulating TAZ. Age and gender-matched littermates expressing only Cre recombinase were used as controls. Spry1 is a critical regulator of adipocyte differentiation and mesenchymal stem cell (MSC) lineage allocation, potentially acting through regulation of CEBP-beta and TAZ.
authors.affiliation: Maine Medical Center Research Institute, 81 Research Dr., Scarborough, ME 04074, USA.
authors.forename: Sumithra
authors.initials: S
authors.lastname: Urs
authors.forename: Deepak
authors.initials: D
authors.lastname: Venkatesh
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Tang
authors.forename: Terry
authors.initials: T
authors.lastname: Henderson
authors.forename: Xuehui
authors.initials: X
authors.lastname: Yang
authors.forename: Robert E
authors.initials: RE
authors.lastname: Friesel
authors.forename: Clifford J
authors.initials: CJ
authors.lastname: Rosen
authors.forename: Lucy
authors.initials: L
authors.lastname: Liaw
mesh_terms: D015502:Absorptiometry, Photon; D048868:Adaptor Proteins, Signal Transducing; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology; D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D006984:Hypertrophy / Q000235:genetics / Q000503:physiopathology; D015151:Immunoblotting; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051879:Twist-Related Protein 1 / Q000235:genetics / Q000378:metabolism; D055114:X-Ray Microtomography
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D022782:CCAAT-Enhancer-Binding Protein-beta; D008565:Membrane Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; C425468:Spry1 protein, mouse; D051879:Twist-Related Protein 1; C067250:Twist1 protein, mouse
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