Identification of HIV integration sites in infected host genomic DNA.
The integration of the Human Immunodeficiency Virus (HIV) genetic information into the host genome is fundamental for its replication and long-term persistence in the host. Isolating and characterizing the integration sites can be useful for obtaining data such as identifying the specific genomic location of integration or understanding the forces dictating HIV integration site selection. The methods outlined in this article describe a highly efficient and precise technique for identifying HIV integration sites in the host genome on a small scale using molecular cloning techniques and standard sequencing or on a massive scale using 454 pyrosequencing.
Ciuffi
Angela
A
Institute of Microbiology, University Hospital Center and University of Lausanne, Bugnon 48, 1011 Lausanne, Switzerland.
Barr
Stephen D
SD
D018929:Cell Culture Techniques; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005814:Genes, Viral; D015894:Genome, Human; D015658:HIV Infections / Q000235:genetics* / Q000821:virology; D015498:HIV-2 / Q000235:genetics / Q000502:physiology*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D054884:Host-Pathogen Interactions; D006801:Humans; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D019562:Viral Load; D014776:Virus Cultivation; D016662:Virus Integration
D004247:DNA