[Effects of storage medium and temperature on short-term storage of Acipenser baerii eggs].
This paper studied the effects of different storage medium (coelomic fluid, Hepes solution, and modified Ringer's solution), temperature (4 degrees C and 16 degrees C), and duration (4 h, 8 h, 16 h, and 24 h) on the short-term storage of Siberian sturgeon (Acipenser baerii) eggs. The results showed that storage medium, temperature, and duration all had significant effects on the fertilization rate, hatching success, and malformation of newly hatched larvae (P < 0.05). With the increase of storage duration, the fertilization and hatching rates decreased, while the malformation rate of hatched larvae increased. The A. baerii eggs stored at 16 degrees C had higher fertilization, hatching, and malformation rates but shorter holding time than those stored at 4 degrees C. The optimal conditions for the short-term storage of A. baerii eggs were Hepes solution prepared according to the biochemical composition of A. baerii coelomic fluid, 16 degrees C, and 4 h, under which, 86.36% fertilization rate, 94.74% hatching rate, and zero malformation would be obtained.
Zhang
Tao
T
Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China. zhangtaoyfi@163.com
Yan
Shi-Wei
SW
Zhuang
Ping
P
Zhang
Long-Zhen
LZ
Tian
Mei-Ping
MP
Wang
Bin
B
Zhang
Da-Hai
DH
Yao
Zhi-Feng
ZF
D000818:Animals; D003470:Culture Media; D005399:Fishes / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D012800:Siberia; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods / Q000662:veterinary*
D003470:Culture Media