A small sequence in domain v of the mitochondrial large ribosomal RNA restores Drosophila melanogaster pole cell determination in uv-irradiated embryos.
The mechanism by which the mitochondrial large rRNA is involved in the restoration of the pole cell-forming ability in Drosophila embryos is still unknown. We identified a 15-ribonucleotide sequence which is conserved from the protobacterium Wolbachia to the higher eukaryotes in domain V of the mitochondrial large rRNA. This short sequence is sufficient to restore pole cell determination in UV-irradiated Drosophila embryos. Here, we provide evidence that the conserved 15-base sequence is sufficient to restore luciferase activity in vitro. Moreover, we show that the internal GAGA sequence is involved in protein binding and that mutations in this tetranucleotide affect the sequence's ability to restore luciferase activity. The obtained results lead us to propose that mtlrRNA may be involved either in damaged protein reactivation or in protein biosynthesis during pole cell determination.
Psaila
Rossana
R
Dipartimento di Biologia Cellulare e Neuroscienze, Istituto Superiore di Sanità Viale Regina Elena, Roma, Italy.
Ponti
Donatella
D
Ponzi
Marta
M
Gigliani
Franca
F
Battaglia
Piero Augusto
PA
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002478:Cells, Cultured; D004331:Drosophila melanogaster / Q000196:embryology* / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000528:radiation effects*; D005260:Female; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D000077278:RNA, Mitochondrial; D012336:RNA, Ribosomal, 16S / Q000737:chemistry* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D014466:Ultraviolet Rays
D000077278:RNA, Mitochondrial; D012336:RNA, Ribosomal, 16S; D016601:RNA-Binding Proteins; D012313:RNA
Mech Dev. 2005 Oct;122(10):1087-93
16125913
J Cell Biol. 1976 Aug;70(2 pt 1):358-73
820700
Eur J Biochem. 2003 Apr;270(8):1599-618
12694174
Cell. 1992 Aug 21;70(4):569-84
1380406
Genes Dev. 1996 Sep 1;10(17):2179-88
8804312
Biochimie. 1987 Sep;69(9):911-23
3126829
Nature. 1992 Jul 30;358(6385):387-92
1641021
Science. 1993 Jun 4;260(5113):1521-4
7684857
Nature. 1994 Feb 3;367(6462):453-5
7906391
Cell. 2000 Sep 1;102(5):615-23
11007480
Dev Biol. 1976 Mar;49(1):40-65
815119
Nucleic Acids Res. 2002 Jun 1;30(11):2390-7
12034826
Genes Genet Syst. 1998 Feb;73(1):1-8
9546203
Curr Top Radiat Res Q. 1976 Mar;11(2):141-99
767058
Int Rev Cytol. 1975;43:229-80
770367
Biochem Biophys Res Commun. 2003 Jun 6;305(3):761-70
12763058
Biochemistry. 2001 Sep 25;40(38):11559-64
11560505
Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8284-7
8710862
Science. 2000 Aug 11;289(5481):905-20
10937989
Science. 1996 Dec 20;274(5295):2075-9
8953037
Science. 2000 Aug 11;289(5481):920-30
10937990
Nucleic Acids Res. 2002 Mar 1;30(5):1278-85
11861922
Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9133-8
11470924
Nature. 1987 Jun 4-10;327(6121):389-94
2953976
Int Rev Cytol. 1974;39:413-79
4611946
J Biol Chem. 1999 Nov 12;274(46):32771-7
10551837
Nature. 1988 Jul 28;334(6180):362-4
2455872
Nucleic Acids Res. 1990 Aug 11;18(15):4592
1697066
Biochim Biophys Acta. 2009 Apr;1789(4):248-9
19056524
Biosci Rep. 2001 Feb;21(1):1-17
11508688
Development. 1989 Dec;107(4):733-42
2483684