Influence of cell-adhesive peptide ligands on poly(ethylene glycol) hydrogel physical, mechanical and transport properties.
Synthetic three-dimensional scaffolds for cell and tissue engineering routinely utilize peptide ligands to provide sites for cell adhesion and to promote cellular activity. Given the fact that recent studies have dedicated great attention to the mechanisms by which cell behavior is influenced by various ligands and scaffold material properties, it is surprising that little work to date has been carried out to investigate the influence of covalently bound ligands on hydrogel material properties. Herein we report the influence of three common ligands utilized in tissue engineering, namely RGD, YIGSR and IKVAV, on the mechanical properties of cross-linked poly(ethylene glycol) (PEG) hydrogels. The effect of the ligands on hydrogel storage modulus, swelling ratio, mesh size and also on the diffusivity of bovine serum albumin through the hydrogel were investigated in detail. We identified conditions under which these ligands strikingly influence the properties of the material. The extent of influence and whether the ligand increases or decreases a specific property is linked to ligand type and concentration. Further, we pinpoint mechanisms by which the ligands interact with the PEG network. This work thus provides specific evidence for interactions between peptide ligands and cross-linked PEG hydrogels that have a significant impact on hydrogel material and transport properties. As a result, this work may have important implications for interpreting cell experiments carried out with ligand-modified hydrogels, because the addition of ligand may affect not only the scaffold's biological properties, but also key physical properties of the system.
Zustiak
Silviya P
SP
Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA.
Durbal
Rohan
R
Leach
Jennie B
JB
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D004058:Diffusion / Q000187:drug effects; D055119:Elastic Modulus / Q000187:drug effects; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry / Q000494:pharmacology*; D006860:Hydrogen Bonding / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008024:Ligands; D008422:Materials Testing; D055595:Mechanical Phenomena / Q000187:drug effects*; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000378:metabolism
D008024:Ligands; D009842:Oligopeptides; D010455:Peptides; D011108:Polymers; C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols
J Control Release. 2014 Sep 28;190:31-2
25356469
Nat Mater. 2004 Apr;3(4):249-53
15034559
Cell Mol Life Sci. 2003 Jan;60(1):119-32
12613662
Biochem Biophys Res Commun. 1992 Feb 14;182(3):1025-31
1540151
Biophys J. 2005 Aug;89(2):1374-88
15923238
Biomaterials. 1998 Jul;19(14):1287-94
9720892
Cell. 2006 Aug 25;126(4):677-89
16923388
J Biomed Mater Res. 1989 Oct;23(10):1183-93
2808463
Bioconjug Chem. 2001 Nov-Dec;12(6):1051-6
11716699
Science. 1987 Oct 23;238(4826):491-7
2821619
Biomaterials. 2003 Nov;24(24):4385-415
12922151
Annu Rev Cell Dev Biol. 1996;12:697-715
8970741
Science. 2005 Nov 18;310(5751):1139-43
16293750
J Biomed Mater Res. 1998 Winter;43(4):422-7
9855200
J Biomed Mater Res A. 2003 Sep 1;66(3):698-706
12918054
Proc Natl Acad Sci U S A. 1984 Oct;81(19):5985-8
6237366
Biomaterials. 2007 Jan;28(1):78-88
16962168
EMBO J. 1985 Oct;4(10):2519-24
2414098
Biotechnol Bioeng. 2003 Jun 5;82(5):578-89
12652481
Acta Biomater. 2007 Sep;3(5):615-29
17572164
Biomacromolecules. 2003 May-Jun;4(3):713-22
12741789
J Mater Sci Mater Med. 1999 Mar;10(3):129-34
15348160
Biomaterials. 2008 Jun;29(18):2757-66
18396331
J Cell Biol. 1995 May;129(3):591-603
7730398
Biophys J. 2000 Jul;79(1):144-52
10866943
Front Neuroeng. 2009 Jun 09;2:6
19668707
J Biol Chem. 1989 Jan 25;264(3):1437-42
2521482
Biomaterials. 2006 May;27(15):2962-9
16445976
Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5413-8
12686696
Nat Biotechnol. 2000 Apr;18(4):415-9
10748522
Dev Biol. 1991 Jun;145(2):266-76
2040373
Biomaterials. 2005 Jan;26(2):167-74
15207463
Biomaterials. 2001 May;22(9):929-41
11311012
Tissue Eng. 2007 May;13(5):1025-34
17417949
Biomacromolecules. 2010 May 10;11(5):1348-57
20355705
Cancer Cell. 2005 Sep;8(3):241-54
16169468
Biophys J. 2004 Jan;86(1 Pt 1):617-28
14695306
J Biomed Mater Res A. 2004 Mar 15;68(4):704-16
14986325
Cell. 1989 Sep 8;58(5):933-43
2528412
Biomaterials. 1993 Dec;14(15):1117-26
8130315
Biomaterials. 2003 Nov;24(24):4353-64
12922148
J Biomed Mater Res. 1998 Feb;39(2):266-76
9457557
Nature. 1984 May 3-9;309(5963):30-3
6325925
Biomaterials. 2001 Nov;22(22):3045-51
11575479
Biotechnol Bioeng. 2003 Jun 30;82(7):784-90
12701144
J Biomed Mater Res A. 2005 Jan 1;72(1):91-7
15536643