Transcriptional profiling of ESTs responsive to Rhizobium vitis from 'Tamnara' grapevines (Vitis sp.).
Genes related with defense responses were screened from the cDNA library constructed with Rhizobium vitis-inoculated or salicylic acid (SA)-treated 'Tamnara' grapevine (Vitis sp.) leaves. Among 13,728 expressed sequence tags (ESTs) from 'Tamnara' grapevine upon R. vitis inoculation and SA treatment, 6776 unigenes containing 1915 contigs and 4860 singletons were obtained. In gene ontology analysis, there were about 3200 clones related with biological process, 3555 with molecular function, and 3354 with cellular component genes. Proteins of secretory organ (35%), plasma membrane (30%), endoplasmic reticulum (20%), and vacuole (11%) were predicted. Photosynthesis-related genes and defense-related genes were most abundant. Among ESTs, 199 resistance-related ones were mapped to the genome of Vitis vinifera L. with three markers, GLP1-12, MHD98, and MHD145, which are known to be linked to resistance against powdery mildew. Approximately, 120 simple sequence repeats (SSRs) detected in cDNAs could be used as EST-derived SSR markers in disease resistant grape breeding.
Choi
Youn Jung
YJ
Fruits Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 440-706, Republic of Korea.
Yun
Hae Keun
HK
Park
Kyo Sun
KS
Noh
Jeong Ho
JH
Heo
Youn Young
YY
Kim
Seung Hui
SH
Kim
Dae Won
DW
Lee
Hee Jae
HJ
D001483:Base Sequence; D002874:Chromosome Mapping; D020224:Expressed Sequence Tags / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D018598:Minisatellite Repeats / Q000235:genetics; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000187:drug effects; D018749:RNA, Plant / Q000235:genetics; D012231:Rhizobium / Q000187:drug effects / Q000502:physiology*; D020156:Salicylic Acid / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014158:Transcription, Genetic; D027843:Vitis / Q000187:drug effects / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*
D010940:Plant Proteins; D018749:RNA, Plant; D020156:Salicylic Acid