Affibody molecules: engineered proteins for therapeutic, diagnostic and biotechnological applications.
Affibody molecules are a class of engineered affinity proteins with proven potential for therapeutic, diagnostic and biotechnological applications. Affibody molecules are small (6.5 kDa) single domain proteins that can be isolated for high affinity and specificity to any given protein target. Fifteen years after its discovery, the Affibody technology is gaining use in many groups as a tool for creating molecular specificity wherever a small, engineering compatible tool is warranted. Here we summarize recent results using this technology, propose an Affibody nomenclature and give an overview of different HER2-specific Affibody molecules. Cumulative evidence suggests that the three helical scaffold domain used as basis for these molecules is highly suited to create a molecular affinity handle for vastly different applications.
Löfblom
J
J
Department of Molecular Biotechnology, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Center, Stockholm, Sweden.
Feldwisch
J
J
Tolmachev
V
V
Carlsson
J
J
Ståhl
S
S
Frejd
F Y
FY
D000345:Affinity Labels / Q000737:chemistry / Q000627:therapeutic use*; D000595:Amino Acid Sequence; D000818:Animals; D001709:Biotechnology; D006801:Humans; D057054:Molecular Imaging; D008969:Molecular Sequence Data; D015202:Protein Engineering; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000627:therapeutic use*; D009626:Terminology as Topic
D000345:Affinity Labels; D018719:Erb-b2 Receptor Tyrosine Kinases; D011994:Recombinant Proteins; C508053:ERBB2 protein, human