Grid analysis improves reliability in follicle counts made by ultrasonography in women with polycystic ovary syndrome.
Poor reliability has been reported when counting the total number of follicles in polycystic ovaries using conventional two-dimensional (2-D) ultrasound viewing methods. In the current study, we report good reliability in follicle counts when observers imposed a programmable grid system over the viewing window. Four observers estimated total follicle counts in 45 ovarian ultrasound scans by compartmentalizing the ovary into 9 to 12 grid sections and performing focused follicle counts per section. The mean number of follicles counted per ovary was 44.6 +/- 2.3. The level of inter-observer agreement when making follicle counts was 0.82 and total follicle counts did not differ among observers. The level of intra-observer agreement was 0.93 which further corroborated the utility of this method for making dependable follicle counts. In summary, the ability to obtain reproducible follicle counts will help to establish reliable diagnostic criteria for polycystic ovarian morphology.
Lujan
Marla E
ME
Division of Nutritional Sciences, College of Agriculture and Life Sciences, Cornell University, Ithaca, New York 14853, USA. MEL245@cornell.edu
Brooks
Eric D
ED
Kepley
Anna L
AL
Chizen
Donna R
DR
Pierson
Roger A
RA
Peppin
Andrew K
AK
D000293:Adolescent; D000328:Adult; D000465:Algorithms; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008954:Models, Biological; D006080:Ovarian Follicle / Q000000981:diagnostic imaging*; D011085:Polycystic Ovary Syndrome / Q000000981:diagnostic imaging*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014463:Ultrasonography / Q000379:methods*; D055815:Young Adult
Hum Reprod. 2007 Mar;22(3):635-43
17204526
Hum Reprod. 2003 Mar;18(3):598-603
12615832
Eur J Endocrinol. 2001 Nov;145(5):619-24
11720881
J Clin Endocrinol Metab. 1961 Nov;21:1440-7
13892577
Dentomaxillofac Radiol. 2006 Sep;35(5):334-8
16940481
Ultrasound Obstet Gynecol. 2007 Mar;29(3):335-41
17211895
Reprod Biol Endocrinol. 2008 Jul 18;6:30
18638401
Lancet. 1985 Dec 21-28;2(8469-70):1375-9
2867389
Hum Reprod. 2005 Oct;20(10):2893-8
16006474
Hum Reprod. 2002 Jun;17(6):1616-22
12042287
Fertil Steril. 2010 May 1;93(7):2303-10
19268929
Acta Obstet Gynecol Scand. 2005 Jul;84(7):611-6
15954867
Int J Fertil Womens Med. 2007 Jan-Feb;52(1):41-7
17987887
Annu Int Conf IEEE Eng Med Biol Soc. 2008;2008:4772-5
19163783
Fertil Steril. 2004 Jan;81(1):19-25
14711538
J Ovarian Res. 2009 May 31;2:5
19480717
Hum Reprod. 2004 Jan;19(1):41-7
14688154
Fertil Steril. 2006 Jan;85(1):214-9
16412756
J Ovarian Res. 2009 Jun 10;2:6
19515259
Clin Endocrinol (Oxf). 2007 Nov;67(5):735-42
17760884
Ultrasound Obstet Gynecol. 2002 Sep;20(3):270-5
12230451
Ultrasound Obstet Gynecol. 2009 Apr;33(4):477-83
19212944
Fertil Steril. 2001 Aug;76(2):326-31
11476780
Biometrics. 1989 Mar;45(1):255-68
2720055
Fertil Steril. 2005 Feb;83(2):291-301
15705365