title: Structure of a virulence regulatory factor CvfB reveals a novel winged helix RNA binding module.
abstract: CvfB is a conserved regulatory protein important for the virulence of Staphylococcus aureus. We show here that CvfB binds RNA. The crystal structure of the CvfB ortholog from Streptococcus pneumoniae at 1.4 A resolution reveals a unique RNA binding protein that is formed from a concatenation of well-known structural modules that bind nucleic acids: three consecutive S1 RNA binding domains and a winged helix (WH) domain. The third S1 and the WH domains are required for cooperative RNA binding and form a continuous surface that likely contributes to the RNA interaction. The WH domain is critical to CvfB function and contains a unique sequence motif. Thus CvfB represents a novel assembly of modules for binding RNA.
authors.affiliation: Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Qingping
authors.initials: Q
authors.lastname: Xu
authors.forename: Shinya
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Chikara
authors.initials: C
authors.lastname: Kaito
authors.forename: Carol L
authors.initials: CL
authors.lastname: Farr
authors.forename: Herbert L
authors.initials: HL
authors.lastname: Axelrod
authors.forename: Hsiu-Ju
authors.initials: HJ
authors.lastname: Chiu
authors.forename: Heath E
authors.initials: HE
authors.lastname: Klock
authors.forename: Mark W
authors.initials: MW
authors.lastname: Knuth
authors.forename: Mitchell D
authors.initials: MD
authors.lastname: Miller
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Elsliger
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Deacon
authors.forename: Adam
authors.initials: A
authors.lastname: Godzik
authors.forename: Scott A
authors.initials: SA
authors.lastname: Lesley
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Sekimizu
authors.forename: Ian A
authors.initials: IA
authors.lastname: Wilson
mesh_terms: D000595:Amino Acid Sequence; D051561:Complement C3 Convertase, Alternative Pathway / Q000737:chemistry*; D019077:Helix-Turn-Helix Motifs / Q000235:genetics; D006460:Hemolysin Proteins / Q000737:chemistry; D007700:Kinetics; D008969:Molecular Sequence Data; D009696:Nucleic Acids / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus / Q000378:metabolism; D014774:Virulence
chemical_list: D006460:Hemolysin Proteins; D009696:Nucleic Acids; D016601:RNA-Binding Proteins; D012313:RNA; D051561:Complement C3 Convertase, Alternative Pathway
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