Selective isolation of Yersinia pestis from plague-infected fleas.
We evaluated Yersinia CIN agar for the isolation of Yersinia pestis from infected fleas. CIN media is effective for the differentiation of Y. pestis from flea commensal flora and is sufficiently inhibitory to other bacteria that typically outcompete Y. pestis after 48 h of growth using less selective media.
Sarovich
Derek S
DS
Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, USA.
Colman
Rebecca E
RE
Price
Erin P
EP
Chung
Wai Kwan
WK
Lee
Judy
J
Schupp
James M
JM
Cobble
Kacy R
KR
Busch
Joseph D
JD
Alexander
James
J
Keim
Paul
P
Wagner
David M
DM
D000818:Animals; D003470:Culture Media / Q000378:metabolism; D007303:Insect Vectors / Q000382:microbiology; D010930:Plague / Q000382:microbiology*; D005423:Siphonaptera / Q000382:microbiology*; D015010:Yersinia pestis / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism
D003470:Culture Media
J Med Res. 1917 Jul;36(3):519-32
19972379
J Gen Microbiol. 1958 Oct;19(2):305-11
13587896
Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8408-13
15173603
J Clin Microbiol. 1999 Jun;37(6):1980-4
10325359
Am J Trop Med Hyg. 2000 May;62(5):552-60
11289663
Adv Exp Med Biol. 2003;529:467-8
12756811
J Bacteriol. 1963 Jun;85:1443-5
14047245
J Med Entomol. 1989 Jul;26(4):342-8
2769715
Biotechniques. 2004 Nov;37(5):730, 732, 734
15560126
Am J Trop Med Hyg. 1956 Nov;5(6):1058-70
13381882
J Bacteriol. 2000 May;182(10):2928-36
10781564
J Clin Microbiol. 1993 Jun;31(6):1511-4
8314993
Can J Microbiol. 1979 Nov;25(11):1298-1304
540256
J Med Entomol. 2000 May;37(3):422-6
15535587
J Med Entomol. 2003 May;40(3):329-37
12943112
J Med Entomol. 1993 Jul;30(4):772-80
8360901
Am J Trop Med Hyg. 1960 Jan;9:91-5
14435458
Trans R Soc Trop Med Hyg. 1996 Nov-Dec;90(6):651
9015505
Am J Trop Med Hyg. 1998 May;58(5):562-9
9598442
Appl Environ Microbiol. 2003 Oct;69(10):5787-92
14532026
Bull World Health Organ. 1956;14(3):457-509
13342926