ELISA measurement for urinary 3-hydroxyproline-containing peptides and its preliminary application to healthy persons and cancer patients.
OBJECTIVE
We reported that endogenous urinary 3-hydroxyproline (3-Hyp) is useful for cancer screening because cancer invasion involves the destruction of basement membrane. A simple and sensitive assay is desired.


METHODS
An ELISA method using a specific antibody against a synthetic peptide of 10 amino acids including 3-Hyp corresponding to the amino acid sequences of collagen type IV alpha chain was applied to urine samples from 180 healthy controls and 22 cancer patients.


RESULTS
The values in controls were 2.44+/-1.90 (SD) mg peptide/g creatinine for 52 men and 2.87+/-2.01 for 128 women, while the values in 22 cancer patients were very low at 0.110+/-0.137 (p<0.001).


CONCLUSIONS
The discrepancy in the data between our previous and present studies is based on the difference of targets measured. 3-Hyp-containing peptides in cancer patients might be destroyed by the elevated peptidase levels found in these patients.


CONCLUSIONS
This ELISA assay may be useful for cancer screening.
Saito
Junichi
J
Department of Internal Medicine, Sanno Hospital, International University of Health and Welfare, Minato-Ku, Tokyo 107-0052, Japan. iokazaki@iuhw.ac.jp.
Imamura
Yasutada
Y
Itoh
Joji
J
Matsuyama
Shigeo
S
Maruta
Akie
A
Hayashi
Toshihiko
T
Sato
Ataru
A
Wada
Norihito
N
Kashiwazaki
Kazuo
K
Inagaki
Yutaka
Y
Watanabe
Tetsu
T
Kitagawa
Yuko
Y
Okazaki
Isao
I
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000652:urine*; D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000652:urine*; D008297:Male; D008403:Mass Screening; D008875:Middle Aged; D009369:Neoplasms / Q000652:urine*; D010190:Pancreatic Neoplasms / Q000652:urine; D010455:Peptides / Q000652:urine*; D013274:Stomach Neoplasms / Q000652:urine
D000906:Antibodies; D014408:Biomarkers, Tumor; D010455:Peptides; D006909:Hydroxyproline