title: [In vitro insecticidal activity of seed neem oil on Lutzomyia longipalpis (Diptera: Psychodidae)].
abstract: Lutzomyia longipalpis is the main vector of visceral leishmaniasis in Brazil. The objective was to evaluate the effect of oil from (Azadirachta indica) neem seeds on eggs, larvae and adults of the vector. The insects were captured in the field and kept in the laboratory at +/- 27 °C and 80% relative humidity. Five treatments with different concentrations were performed using two negative controls (distilled water and Tween 80) and a positive control. The eggs were sprayed with the oil at different concentrations and the number of hatched larvae evaluated for 10 days. Mortality of larvae was observed to pupation and adult mortality was observed after 24, 48, and 72 hours. Statistical analysis was performed by Tukey test at 5% probability. The highest oil concentration of eggs obtained 65.16 +/- 3.24% efficacy for reducing egg hatching. The test with larvae showed 67.75 +/- 2.21% efficacy at a concentration of 100 mg.mL⁻¹. In adults, the efficacy of the 100 mg.mL⁻¹ concentration was 96.64 +/- 4.11% after 24 hours. The phytochemical analysis revealed the presence of triterpenes. These results demonstrate the potential use of this oil in the control of this vector.
authors.affiliation: Programa de Pós-Graduação em Ciências Veterinárias,Laboratório de Doenças Parasitárias, Universidade Estadual do Ceará UECEAv. Paranjana, 1.700, CEP 60.740-000, Fortaleza, Ceará, Brazil. claudiamlb@yahoo.com.br.
authors.forename: Michelline V
authors.initials: MV
authors.lastname: Maciel
authors.forename: Selene M
authors.initials: SM
authors.lastname: Morais
authors.forename: Claudia M L
authors.initials: CM
authors.lastname: Bevilaqua
authors.forename: Rafaella A
authors.initials: RA
authors.lastname: Silva
authors.forename: Renata S
authors.initials: RS
authors.lastname: Barros
authors.forename: Raimundo N
authors.initials: RN
authors.lastname: Sousa
authors.forename: Lindemberg C
authors.initials: LC
authors.lastname: Sousa
authors.forename: Lyeghyna K A
authors.initials: LK
authors.lastname: Machado
authors.forename: Edy S
authors.initials: ES
authors.lastname: Brito
authors.forename: Manoel A
authors.initials: MA
authors.lastname: Souza-Neto
mesh_terms: D000818:Animals; D005989:Glycerides / Q000494:pharmacology*; D007300:Insect Control / Q000379:methods*; D010938:Plant Oils / Q000494:pharmacology*; D011576:Psychodidae / Q000187:drug effects* / Q000254:growth & development; D013729:Terpenes / Q000494:pharmacology*
chemical_list: D005989:Glycerides; D010938:Plant Oils; D013729:Terpenes; C002443:neem oil