title: Preliminary X-ray crystallographic analysis of the breakage-reunion domain of the GyrA subunit of DNA gyrase from Colwellia psychrerythraea strain 34H.
abstract: DNA gyrase is a type II topoisomerase that is essential for chromosome segregation and cell division owing to its ability to modify the topological forms of bacterial DNA. In this study, the N-terminal breakage-reunion domain of the GyrA subunit of DNA gyrase from Colwellia psychrerythraea 34H was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.60 A resolution using a synchrotron-radiation source. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 98.98, b = 101.56, c = 141.83 A. The asymmetric unit contained two molecules, with a corresponding V(M) of 3.18 A(3) Da(-1) and a solvent content of 59.9%.
authors.affiliation: Department of Chemistry, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Ha Yun
authors.initials: HY
authors.lastname: Jung
authors.forename: Kyung Ha
authors.initials: KH
authors.lastname: Kim
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Hyoung
authors.forename: Mi Ra
authors.initials: MR
authors.lastname: Han
authors.forename: Hyun Kyoung
authors.initials: HK
authors.lastname: Kim
authors.forename: Ki Jeung
authors.initials: KJ
authors.lastname: Lee
authors.forename: Yangmee
authors.initials: Y
authors.lastname: Kim
authors.forename: Hak Jun
authors.initials: HJ
authors.lastname: Kim
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Heo
mesh_terms: D034241:Alteromonadaceae / Q000201:enzymology*; D018360:Crystallography, X-Ray; D027081:DNA Gyrase / Q000737:chemistry*
chemical_list: D027081:DNA Gyrase
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