Proteomic and functional characterization of a Chlamydomonas reinhardtii mutant lacking the mitochondrial alternative oxidase 1.
In the present work, we have isolated by RNA interference and characterized at the functional and the proteomic levels a Chlamydomonas reinhardtii strain devoid of the mitochondrial alternative oxidase 1 (AOX1). The AOX1-deficient strain displays a remarkable doubling of the cell volume and biomass without alteration of the generation time or change in total respiratory rate, with a significantly higher ROS production. To identify the molecular adaptation underlying these observations, we have carried out a comparative study of both the mitochondrial and the cellular soluble proteomes. Our results indicate a strong up-regulation of the ROS scavenging systems and important quantitative modifications of proteins involved in the primary metabolism, namely an increase of enzymes involved in anabolic pathways and a concomitant general down-regulation of enzymes of the main catabolic pathways.
Mathy
Grégory
G
Laboratory of Bioenergetics and Cellular Physiology, University of Liege, Belgium.
Cardol
Pierre
P
Dinant
Monique
M
Blomme
Arnaud
A
Gérin
Stéphanie
S
Cloes
Marie
M
Ghysels
Bart
B
DePauw
Edwin
E
Leprince
Pierre
P
Remacle
Claire
C
Sluse-Goffart
Claudine
C
Franck
Fabrice
F
Matagne
René F
RF
Sluse
Francis E
FE
D015153:Blotting, Western; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D020868:Gene Silencing / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010788:Photosynthesis; D010940:Plant Proteins; D011506:Proteins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction; D013347:Subcellular Fractions / Q000378:metabolism; D000099188:Alternative Oxidase
D006861:Hydrogen Peroxide; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D011506:Proteins; D020543:Proteome; D000099188:Alternative Oxidase