AlphaE-catenin regulates actin dynamics independently of cadherin-mediated cell-cell adhesion.
alphaE-catenin binds the cell-cell adhesion complex of E-cadherin and beta-catenin (beta-cat) and regulates filamentous actin (F-actin) dynamics. In vitro, binding of alphaE-catenin to the E-cadherin-beta-cat complex lowers alphaE-catenin affinity for F-actin, and alphaE-catenin alone can bind F-actin and inhibit Arp2/3 complex-mediated actin polymerization. In cells, to test whether alphaE-catenin regulates actin dynamics independently of the cadherin complex, the cytosolic alphaE-catenin pool was sequestered to mitochondria without affecting overall levels of alphaE-catenin or the cadherin-catenin complex. Sequestering cytosolic alphaE-catenin to mitochondria alters lamellipodia architecture and increases membrane dynamics and cell migration without affecting cell-cell adhesion. In contrast, sequestration of cytosolic alphaE-catenin to the plasma membrane reduces membrane dynamics. These results demonstrate that the cytosolic pool of alphaE-catenin regulates actin dynamics independently of cell-cell adhesion.
Benjamin
Jacqueline M
JM
Cancer Biology Program, Stanford University, Stanford, CA 94305, USA.
Kwiatkowski
Adam V
AV
Yang
Changsong
C
Korobova
Farida
F
Pokutta
Sabine
S
Svitkina
Tatyana
T
Weis
William I
WI
Nelson
W James
WJ
D051376:Actin-Related Protein 2-3 Complex / Q000235:genetics / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002465:Cell Movement / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism; D004285:Dogs; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D055503:Protein Multimerization; D011554:Pseudopodia / Q000378:metabolism; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D051178:alpha Catenin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism
D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D015820:Cadherins; D011993:Recombinant Fusion Proteins; D051178:alpha Catenin; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases
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