title: Genomic alterations of primary tumor and blood in invasive ductal carcinoma of breast.
abstract: BACKGROUND
  Genomic alterations are important events in the origin and progression of various cancers, with DNA copy number changes associated with progression and treatment response in cancer. Array CGH is potentially useful in the identification of genomic alterations from primary tumor and blood in breast cancer patients. The aim of our study was to compare differences of DNA copy number changes in blood and tumor tissue in breast cancer.
  
  
  METHODS
  DNA copy number changes in blood were compared to those in tumor tissue using array-comparative genomic hybridization in samples obtained from 30 breast cancer patients. The relative degree of chromosomal changes was analyzed using log2 ratios and data was validated by real-time polymerase chain reaction.
  
  
  RESULTS
  Forty-six regions of gains present in more than 30% of the tissues and 70 regions of gains present in more than 30% of blood were identified. The most frequently gained region was chromosome 8q24. In total, agreement of DNA copy numbers between primary tumor and blood was minimal (Kappa = 0.138, p < 0.001).
  
  
  CONCLUSIONS
  Although there was only a slight agreement of DNA copy number alterations between the primary tumor and the blood samples, the blood cell copy number variation may have some clinical significance as compared to the primary tumor in IDC breast cancer patients.
authors.affiliation: Department of Surgery, The Catholic University, Seoul, Korea.
authors.forename: Ja Seong
authors.initials: JS
authors.lastname: Bae
authors.forename: Jin Soo
authors.initials: JS
authors.lastname: Choi
authors.forename: Seung Ho
authors.initials: SH
authors.lastname: Baik
authors.forename: Woo Chan
authors.initials: WC
authors.lastname: Park
authors.forename: Byung Joo
authors.initials: BJ
authors.lastname: Song
authors.forename: Jeong Soo
authors.initials: JS
authors.lastname: Kim
authors.forename: Young
authors.initials: Y
authors.lastname: Lim
authors.forename: Sang Seol
authors.initials: SS
authors.lastname: Jung
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D055028:Comparative Genomic Hybridization; D056915:DNA Copy Number Variations / Q000235:genetics*; D004273:DNA, Neoplasm / Q000097:blood*; D005260:Female; D015894:Genome, Human; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011379:Prognosis
chemical_list: D004273:DNA, Neoplasm
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