title: Post-challenge hematological evaluation with virulent strain of Eimeria tenella in broilers immunized with attenuated strain or sporozoite proteins from homologous strain.
abstract: Hematological parameters were evaluated in broilers immunized and challenged with Eimeria tenella. Broiler chickens of Hubbard strain, females, coccidian-free, were kept in wire cages and inoculated on the third day. The experiment was designed to include five sorts of treatment with three replicates each. T1 was the negative control group, T2 received 500 attenuated sporulated oocysts by gavage, T3 was the positive control, T4 received 50 μg of sporozoite protein + Quil A vaccine, and T5 received Quil A without sporozoite protein + PBS, the last two through nasal route on days 0, 7, and 21. On the 31st day, all treatments were challenged with homologous virulent strain of E. tenella in the dose of 8.0 x 10⁴ oocysts, with the exception of T1. One week later, blood sampling, lesion scores, and cecal oocyst count were carried out. The parasitological parameters showed statistical significance (p < 0.05) and there was no damage to the hematological parameters of birds (p > 0.05) by ANOVA test. The correlations suggest that the blood parameters were impaired by effects of the parasite on tissue, showing levels of hemorrhage and/or hydration.
authors.affiliation: Departamento de Medicina Veterinária Preventiva,Centro de Ciências Agrárias, Universidade Estadual de Londrina UEL,Campus Universitário, Rod. Celso Garcia Cid, PR 445, km 380,CP 6001, CEP 86051-990 Londrina-PR, Brazil. alexey@yahoo.com.br
authors.forename: Alexey Leon Gomel
authors.initials: AL
authors.lastname: Bogado
authors.forename: João Luis
authors.initials: JL
authors.lastname: Garcia
authors.forename: Patrícia Fernandes Nunes da
authors.initials: PF
authors.lastname: Silva
authors.forename: Mara Regina Stipp
authors.initials: MR
authors.lastname: Balarin
authors.forename: José da Silva Guimarães
authors.initials: Jda S
authors.lastname: Junior
mesh_terms: D000818:Animals; D002645:Chickens / Q000097:blood* / Q000276:immunology*; D016786:Eimeria tenella / Q000276:immunology*; D005260:Female; D007114:Immunization; D015800:Protozoan Proteins / Q000276:immunology*; D034101:Sporozoites / Q000276:immunology*
chemical_list: D015800:Protozoan Proteins