Genetic diversity analysis in Piper species (Piperaceae) using RAPD markers.
The genetic diversity of eight species of Piper (Piperaceae) viz., P. nigrum, P. longum, P. betle, P. chaba, P. argyrophyllum, P. trichostachyon, P. galeatum, and P. hymenophyllum from Kerala state, India were analyzed by Random amplified polymorphic DNA (RAPD). Out of 22 10-mer RAPD primers screened, 11 were selected for comparative analysis of different species of Piper. High genetic variations were found among different Piper species studied. Among the total of 149 RAPD fragments amplified, 12 bands (8.05%) were found monomorphic in eight species. The remaining 137 fragments were found polymorphic (91.95%). Species-specific bands were found in all eight species studied. The average gene diversity or heterozygosity (H) was 0.33 across all the species, genetic distances ranged from 0.21 to 0.69. The results of this study will facilitate germplasm identification, management, and conservation.
Sen
Sandeep
S
Molecular Biology and Genetic Engineering Research Unit, School of Biosciences, Mar Athanasios College for Advanced Studies, Tiruvalla (MACFAST), Kerala, India. sensand@gmail.com
Skaria
Reby
R
Muneer
P M A
PMA
D020029:Base Pairing / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D010375:Pedigree; D010802:Phylogeny; D031701:Piper / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity
D005819:Genetic Markers
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