title: Non-woven PGA/PVA fibrous mesh as an appropriate scaffold for chondrocyte proliferation.
abstract: Non-woven textile mesh from polyglycolic acid (PGA) was found as a proper material for chondrocyte adhesion but worse for their proliferation. Neither hyaluronic acid nor chitosan nor polyvinyl alcohol (PVA) increased chondrocyte adhesion. However, chondrocyte proliferation suffered from acidic byproducts of PGA degradation. However, the addition of PVA and/or chitosan into a wet-laid non-woven textile mesh from PGA improved chondrocyte proliferation seeded in vitro on the PGA-based composite scaffold namely due to a diminished acidification of their microenvironment. This PVA/PGA composite mesh used in combination with a proper hydrogel minimized the negative effect of PGA degradation without dropping positive parameters of the PGA wet-laid non-woven textile mesh. In fact, presence of PVA and/or chitosan in the PGA-based wet-laid non-woven textile mesh even advanced the PGA-based wet-laid non-woven textile mesh for chondrocyte seeding and artificial cartilage production due to a positive effect of PVA in such a scaffold on chondrocyte proliferation.
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Rampichová
authors.forename: E
authors.initials: E
authors.lastname: Koštáková
authors.forename: E
authors.initials: E
authors.lastname: Filová
authors.forename: E
authors.initials: E
authors.lastname: Prosecká
authors.forename: M
authors.initials: M
authors.lastname: Plencner
authors.forename: L
authors.initials: L
authors.lastname: Ocheretná
authors.forename: A
authors.initials: A
authors.lastname: Lytvynets
authors.forename: D
authors.initials: D
authors.lastname: Lukáš
authors.forename: E
authors.initials: E
authors.lastname: Amler
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology; D002448:Cell Adhesion; D002455:Cell Division; D019902:Chondrocytes / Q000166:cytology*; D006820:Hyaluronic Acid; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D018613:Microscopy, Confocal; D011100:Polyglycolic Acid; D011142:Polyvinyl Alcohol; D011817:Rabbits; D013784:Textiles; D046509:Tissue Culture Techniques / Q000379:methods*; D054457:Tissue Scaffolds; D014867:Water
chemical_list: D014867:Water; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011100:Polyglycolic Acid; D011142:Polyvinyl Alcohol; D006820:Hyaluronic Acid