title: Nitric Oxide contributes to the regulation of iron metabolism in skeletal muscle in vivo and in vitro.
abstract: Nitric Oxide (NO) plays an important role in iron redistribution during exercise, while its molecular regulatory mechanism is still not clear. Our present studies were to investigate the effects of NO on iron metabolism and to elucidate the regulatory mechanism of iron transport in skeletal muscle both in vivo and in vitro. One group of male Wistar rats (300 +/- 10 g) were subjected to an exercise of 30 min on a treadmill for 5 weeks (exercise group, EG, 6 rats) and the other one was placed on the treadmill without running (control group, CG, 6 rats). The cultured L6 rat skeletal muscle cells were treated with either 0.5 mM SNAP (NO donor) or not for 24 h, and their iron release and intake amount were examined by measuring radiolabelled (55)Fe. The results showed: (1) The NO content (CG, 1.09 +/- 0.18 micromol/g vs. EG, 1.49 +/- 0.17 micromol/g) and non-heme iron in gastrocnemius (CG, 118.35 +/- 11.41 microg/g vs. EG, 216.65 +/- 11.10 microg/g) of EG were significantly increased compared with CG. (2) The expression of DMT1 (IRE) and TfR1 of EG was increased. (3) The iron intake was increased in L6 cells treated with SNAP (P < 0.01). (4) Western blot results showed the protein level of both TfR1 and DMT1 (IRE) in SNAP cells were up-regulated, while the expression of FPN1 was down-regulated (P < 0.05). The data suggested that the induced elevation of NO level by exercise lead to the up-regulation of both TfR1 and DMT1 (IRE), which in turn increasing the iron absorption in skeletal muscle.
authors.affiliation: College of Physical Education, Hebei Normal University, Shijiazhuang, Hebei Province, People's Republic of China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Wang
authors.forename: Xianglin
authors.initials: X
authors.lastname: Duan
authors.forename: Jianguo
authors.initials: J
authors.lastname: Liu
authors.forename: Huanbin
authors.initials: H
authors.lastname: Zhao
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Liu
authors.forename: Yanzhong
authors.initials: Y
authors.lastname: Chang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D027682:Cation Transport Proteins / Q000378:metabolism; D002478:Cells, Cultured; D016166:Free Radical Scavengers / Q000494:pharmacology*; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009211:Myoglobin / Q000378:metabolism; D009569:Nitric Oxide / Q000494:pharmacology*; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar; D011990:Receptors, Transferrin / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D000099236:Solute Carrier Family 11, Member 2
chemical_list: D027682:Cation Transport Proteins; D016166:Free Radical Scavengers; D007501:Iron; D009211:Myoglobin; D009569:Nitric Oxide; D011990:Receptors, Transferrin; D013385:Succinate Dehydrogenase; D000099236:Solute Carrier Family 11, Member 2; C545564:Tfrc protein, rat
references.citation: J Smooth Muscle Res. 2008;44(3-4):101-11
references.pmid: 18832786
references.citation: J Nutr. 2005 Sep;135(9):2151-8
references.pmid: 16140891
references.citation: Blood Cells Mol Dis. 2002 Nov-Dec;29(3):315-26
references.pmid: 12547222
references.citation: J Pharmacol Exp Ther. 2006 Nov;319(2):672-81
references.pmid: 16895977
references.citation: Blood Cells Mol Dis. 2002 Nov-Dec;29(3):400-10
references.pmid: 12547230
references.citation: Nature. 1997 Jul 31;388(6641):482-8
references.pmid: 9242408
references.citation: Mol Cell Biochem. 2006 Sep;289(1-2):175-83
references.pmid: 16718371
references.citation: Mol Cell Biochem. 2010 Jan;333(1-2):191-201
references.pmid: 19618122
references.citation: Crit Rev Clin Lab Sci. 2008;45(1):1-23
references.pmid: 18293179
references.citation: Eur J Appl Physiol. 2008 Nov;104(5):795-802
references.pmid: 18784937
references.citation: Exp Physiol. 2009 Jun;94(6):720-30
references.pmid: 19201785
references.citation: Mol Cell Biochem. 2009 Dec;332(1-2):225-31
references.pmid: 19588229
references.citation: Nature. 2000 Feb 17;403(6771):776-81
references.pmid: 10693807
references.citation: Mol Cell Biochem. 1992 May 13;112(1):45-52
references.pmid: 1325034
references.citation: Gut. 2003 Mar;52(3):340-6
references.pmid: 12584213
references.citation: Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8175-82
references.pmid: 8710843
references.citation: Endocrinology. 2007 Jan;148(1):293-9
references.pmid: 17023529
references.citation: J Cell Biochem. 2008 May 15;104(2):629-41
references.pmid: 18189270
references.citation: Eur J Appl Physiol. 2008 Jul;103(4):381-91
references.pmid: 18365240
references.citation: J Appl Physiol (1985). 2009 Dec;107(6):1685-92
references.pmid: 19729589
references.citation: Mol Cell Biochem. 2003 Oct;252(1-2):65-72
references.pmid: 14577577
references.citation: Exp Physiol. 2008 Mar;93(3):407-14
references.pmid: 17981932
references.citation: Biol Rev Camb Philos Soc. 2002 Nov;77(4):529-36
references.pmid: 12475052
references.citation: Science. 1992 Dec 18;258(5090):1898-902
references.pmid: 1281928
references.citation: J Nutr Biochem. 2009 Nov;20(11):860-5
references.pmid: 19027283
references.citation: J Appl Physiol (1985). 1990 Nov;69(5):1709-17
references.pmid: 1703145
references.citation: Mol Cell Biochem. 2006 Jan;282(1-2):117-23
references.pmid: 16317519
references.citation: Nature. 2008 Nov 27;456(7221):511-5
references.pmid: 18953332
references.citation: Int J Sports Med. 2004 May;25(4):306-13
references.pmid: 15162251
references.citation: Blood. 2007 Jun 1;109(11):4724-31
references.pmid: 17311997
references.citation: Biochem Biophys Res Commun. 2001 Mar 23;282(1):264-7
references.pmid: 11264001
references.citation: J Appl Physiol (1985). 2009 Jun;106(6):1925-34
references.pmid: 19299569