Candida gosingica sp. nov., an anamorphic ascomycetous yeast closely related to Scheffersomyces spartinae.
During surveys on yeast diversity in forest soils from Taiwan and Thailand, ten yeast strains isolated from different samples were found to have similar molecular and physiological characteristics. Sequence analysis of small subunit (SSU) rDNA, the D1/D2 domain of large subunit (LSU) rDNA and internal transcribed spacer (ITS)-5.8S rDNA demonstrated that these strains were closely related to Scheffersomyces spartinae. The novel strains could be differentiated from S. spartinae by a 0.9  % sequence divergence (5 substitutions, 0 gaps) in the D1/D2 domain of LSU rDNA, a 1.5  % divergence (8 substitutions, 0 gaps) in the ITS-5.8S rDNA and a 0.7  % divergence (12 substitutions, 2 gaps) in the SSU rDNA. The novel strains also showed specific patterns of electrophoretic karyotypes that differed from that of S. spartinae. Therefore, a novel yeast species, Candida gosingica sp. nov., is proposed to accommodate these strains. The type strain SJ7S11(T) (=BCRC 23194(T)=CBS 11433(T)) was assigned and deposited in the Bioresource Collection and Research Center (BCRC), Food Industry Development and Research Institute, Hsinchu, Taiwan, and Centraalbureau voor Schimmelcultures (CBS), Utrecht, The Netherlands.
Chang
Chin-Feng
CF
Department of Applied Science, National Hsinchu University of Education, 521 Nanda Rd, Hsinchu 30014, Taiwan.
Yao
Cheng-Hsu
CH
Department of Applied Science, National Hsinchu University of Education, 521 Nanda Rd, Hsinchu 30014, Taiwan.
Young
Shuh-Sen
SS
Department of Applied Science, National Hsinchu University of Education, 521 Nanda Rd, Hsinchu 30014, Taiwan.
Limtong
Savitree
S
Department of Microbiology, Kasetsart University, Bangkok 10900, Thailand.
Kaewwichian
Rungluk
R
Department of Microbiology, Kasetsart University, Bangkok 10900, Thailand.
Srisuk
Nantana
N
Department of Microbiology, Kasetsart University, Bangkok 10900, Thailand.
Lee
Ching-Fu
CF
Department of Applied Science, National Hsinchu University of Education, 521 Nanda Rd, Hsinchu 30014, Taiwan.
D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D007621:Karyotyping; D008969:Molecular Sequence Data; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013624:Taiwan; D013785:Thailand; D014197:Trees
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; C065096:RNA, ribosomal, 26S