Prokineticin 1, homeobox A10, and progesterone receptor messenger ribonucleic acid expression in primary cultures of endometrial stromal cells isolated from endometrium of healthy women and from eutopic endometrium of women with endometriosis.
OBJECTIVE
To examine prokineticin 1 (PROK1), homeobox (HOX) A10, and P receptor (PR) messenger ribonucleic acid (mRNA) expression in primary cultures of endometrial stromal cells (ESC) obtained from eutopic endometrial samples of patients with endometriosis and to clarify whether in vitro steroid hormone dependence of PROK1 gene expression is altered in endometriosis.


METHODS
Prospective laboratory study.


METHODS
Tertiary university hospital.


METHODS
Twelve normal women (controls) and 12 patients affected by moderate to severe endometriosis in the midsecretory phase of the menstrual cycle.


METHODS
Endometrial specimens were obtained from control women and from women affected by endometriosis; ESC were isolated from endometrial biopsies, and primary cultures were established.


METHODS
Real-time polymerase chain reaction analysis of PROK1, HOXA10, and PR mRNA expression in ESC after 1-4 days of steroid hormone treatment and after decidual differentiation.


RESULTS
Contrary to ESC from control women, in ESC obtained from women affected by endometriosis PROK1 and PR mRNA expression was not induced by 1-4 days of treatment with steroid hormones. Nevertheless, when ESC from both groups of women were differentiated to decidual phenotype, PROK1 mRNA was up-regulated and PR and HOXA10 mRNA were down-regulated to the same extent.


CONCLUSIONS
Our results provide additional evidence for P resistance in endometriosis.
Tiberi
Federica
F
Istituto Scientifico Internazionale Paolo VI, Università Cattolica del Sacro Cuore (UCSC), Rome, Italy. fm1810@inwind.it
Tropea
Anna
A
Romani
Federica
F
Apa
Rosanna
R
Marana
Riccardo
R
Lanzone
Antonio
A
D000328:Adult; D016022:Case-Control Studies; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003656:Decidua / Q000378:metabolism / Q000502:physiology; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005768:Gastrointestinal Hormones / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006262:Health; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011980:Receptors, Progesterone / Q000235:genetics* / Q000378:metabolism; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D014591:Uterine Diseases / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D042662:Vascular Endothelial Growth Factor, Endocrine-Gland-Derived / Q000235:genetics* / Q000378:metabolism
D005768:Gastrointestinal Hormones; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; C467610:PROK1 protein, human; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D042662:Vascular Endothelial Growth Factor, Endocrine-Gland-Derived; C069634:HOXA10 protein, human; D004958:Estradiol