title: Binding and release characteristics of insulin-like growth factor-1 from a collagen-glycosaminoglycan scaffold.
abstract: Tissue engineering is a promising technique for cartilage repair, but to optimize novel scaffolds before clinical trials, it is necessary to determine their characteristics for binding and release of growth factors. Toward this goal, a novel, porous collagen-glycosaminoglycan scaffold was loaded with a range of concentrations of insulin-like growth factor-1 (IGF-1) to evaluate its potential as a controlled delivery device. The kinetics of IGF-1 adsorption and release from the scaffold was demonstrated using radiolabeled IGF-1. Adsorption was rapid, and was approximately proportional to the loading concentration. Ionic bonding contributed to this interaction. IGF-1 release was studied over 14 days to compare the release profiles from different loading groups. Two distinct phases occurred: first, a burst release of up to 44% was noted within the first 24 h; then, a slow, sustained release (13%-16%) was observed from day 1 to 14. When the burst release was subtracted, the relative percentage of remaining IGF-1 released was similar for all loading groups and broadly followed t(½) kinetics until approximately day 6. Scaffold cross-linking using dehydrothermal treatment did not affect IGF-1 adsorption or release. Bioactivity of released IGF-1 was confirmed by seeding scaffolds (preadsorbed with unlabeled IGF-1) with human osteoarthritic chondrocytes and demonstrating increased proteoglycan production in vitro.
authors.affiliation: Department of Materials Science and Metallurgy, University of Cambridge, Cambridge, UK.
authors.forename: Leanne M
authors.initials: LM
authors.lastname: Mullen
authors.forename: Serena M
authors.initials: SM
authors.lastname: Best
authors.forename: Roger A
authors.initials: RA
authors.lastname: Brooks
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Ghose
authors.forename: Jessica H
authors.initials: JH
authors.lastname: Gwynne
authors.forename: John
authors.initials: J
authors.lastname: Wardale
authors.forename: Neil
authors.initials: N
authors.lastname: Rushton
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Cameron
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D015918:Infusion Pumps, Implantable; D007334:Insulin-Like Growth Factor I / Q000378:metabolism* / Q000493:pharmacokinetics*; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D011485:Protein Binding / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D006025:Glycosaminoglycans; D007334:Insulin-Like Growth Factor I; D003094:Collagen
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