Probing single nanometer-scale pores with polymeric molecular rulers.
We previously demonstrated that individual molecules of single-stranded DNA can be driven electrophoretically through a single Staphylococcus aureus alpha-hemolysin ion channel. Polynucleotides thread through the channel as extended chains and the polymer-induced ionic current blockades exhibit stable modes during the interactions. We show here that polynucleotides can be used to probe structural features of the alpha-hemolysin channel itself. Specifically, both the pore length and channel aperture profile can be estimated. The results are consistent with the channel crystal structure and suggest that polymer-based "molecular rulers" may prove useful in deducing the structures of nanometer-scale pores in general.
Henrickson
Sarah E
SE
Semiconductor Electronics Division, NIST, Bldg. 225, Room B326, Gaithersburg, Maryland 20899-8120, USA.
DiMarzio
Edmund A
EA
Wang
Qian
Q
Stanford
Vincent M
VM
Kasianowicz
John J
JJ
D001692:Biological Transport; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry*; D016062:Porosity
D004277:DNA, Single-Stranded; D006460:Hemolysin Proteins; D015335:Molecular Probes
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