title: Rapid diagnosis of Old World Leishmaniasis by high-resolution melting analysis of the 7SL RNA gene.
abstract: High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World Leishmania was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (P < 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.
authors.affiliation: Department of Microbiology and Molecular Genetics, IMIRC, the Kuvin Center for the Study of Infectious and Tropical Diseases, the Hebrew University-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel.
authors.forename: Abedelmajeed
authors.initials: A
authors.lastname: Nasereddin
authors.forename: Charles L
authors.initials: CL
authors.lastname: Jaffe
mesh_terms: D017931:DNA Primers / Q000235:genetics; D006801:Humans; D007891:Leishmania / Q000235:genetics* / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000469:parasitology; D010274:Parasitology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016053:RNA, Protozoan / Q000235:genetics*; D020733:RNA, Small Cytoplasmic / Q000235:genetics*; D012680:Sensitivity and Specificity; D018271:Signal Recognition Particle / Q000235:genetics*; D044366:Transition Temperature
chemical_list: C115990:7SL RNA; D017931:DNA Primers; D016053:RNA, Protozoan; D020733:RNA, Small Cytoplasmic; D018271:Signal Recognition Particle
references.citation: Cancer Epidemiol Biomarkers Prev. 2008 May;17(5):1240-7
references.pmid: 18483346
references.citation: Diagn Microbiol Infect Dis. 2003 Sep;47(1):349-58
references.pmid: 12967749
references.citation: Clin Chem. 2008 Feb;54(2):432-6
references.pmid: 18223135
references.citation: Nat Genet. 2008 Jun;40(6):782-8
references.pmid: 18469812
references.citation: Int J Parasitol. 2006 Feb;36(2):237-46
references.pmid: 16307745
references.citation: PLoS Negl Trop Dis. 2010 Jan 12;4(1):e581
references.pmid: 20069036
references.citation: Am J Trop Med Hyg. 2005 Apr;72(4):415-20
references.pmid: 15827278
references.citation: Nucleic Acids Res. 1988 Nov 25;16(22):10881-90
references.pmid: 2849754
references.citation: J Clin Microbiol. 2008 Apr;46(4):1369-73
references.pmid: 18305131
references.citation: J Clin Microbiol. 2008 Sep;46(9):2848-55
references.pmid: 18614659
references.citation: Methods Mol Biol. 2000;132:365-86
references.pmid: 10547847
references.citation: J Clin Microbiol. 2006 Apr;44(4):1435-9
references.pmid: 16597873