A variant of the KLK4 gene is expressed as a cis sense-antisense chimeric transcript in prostate cancer cells.
In humans, more than 30,000 chimeric transcripts originating from 23,686 genes have been identified. The mechanisms and association of chimeric transcripts arising from chromosomal rearrangements with cancer are well established, but much remains unknown regarding the biogenesis and importance of other chimeric transcripts that arise from nongenomic alterations. Recently, a SLC45A3-ELK4 chimera has been shown to be androgen-regulated, and is overexpressed in metastatic or high-grade prostate tumors relative to local prostate cancers. Here, we characterize the expression of a KLK4 cis sense-antisense chimeric transcript, and show other examples in prostate cancer. Using non-protein-coding microarray analyses, we initially identified an androgen-regulated antisense transcript within the 3' untranslated region of the KLK4 gene in LNCaP cells. The KLK4 cis-NAT was validated by strand-specific linker-mediated RT-PCR and Northern blotting. Characterization of the KLK4 cis-NAT by 5' and 3' rapid amplification of cDNA ends (RACE) revealed that this transcript forms multiple fusions with the KLK4 sense transcript. Lack of KLK4 antisense promoter activity using reporter assays suggests that these transcripts are unlikely to arise from a trans-splicing mechanism. 5' RACE and analyses of deep sequencing data from LNCaP cells treated +/-androgens revealed six high-confidence sense-antisense chimeras of which three were supported by the cDNA databases. In this study, we have shown complex gene expression at the KLK4 locus that might be a hallmark of cis sense-antisense chimeric transcription.
Lai
John
J
Australian Prostate Cancer Research Centre-Queensland, Queensland University of Technology and Princess Alexandra Hospital, Woolloongabba, Queensland 4102, Australia.
Lehman
Melanie L
ML
Dinger
Marcel E
ME
Hendy
Stephen C
SC
Mercer
Tim R
TR
Seim
Inge
I
Lawrence
Mitchell G
MG
Mattick
John S
JS
Clements
Judith A
JA
Nelson
Colleen C
CC
D000951:Antigens, Neoplasm / Q000235:genetics; D002678:Chimera / Q000235:genetics; D002874:Chromosome Mapping; D016373:DNA, Antisense / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005091:Exons; D015321:Gene Rearrangement; D014644:Genetic Variation; D055106:Genome-Wide Association Study; D006801:Humans; D007610:Kallikreins / Q000235:genetics*; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D014158:Transcription, Genetic; D050808:ets-Domain Protein Elk-4 / Q000235:genetics
D000951:Antigens, Neoplasm; D016373:DNA, Antisense; D004273:DNA, Neoplasm; C494193:ELK4 protein, human; D026901:Membrane Transport Proteins; C510061:SLC45A2 protein, human; D050808:ets-Domain Protein Elk-4; D007610:Kallikreins; C120801:kallikrein 4
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