title: [Effect of periodontal mechanical treatment on periodontal pathogenic bacteria in gingival crevicular fluid of chronic periodontitis patients].
abstract: OBJECTIVE
  To evaluate the subgingival prevalent rates of 6 periodontal pathogenic bacteria in gingival crevicular fluids of CP patients before and after treatment, to analyze the relationship between the prevalent variance and periodontal clinical parameters, and to provide a microbiologic method of evaluating curative effect and estimating the prognosis.
  
  
  METHODS
  Gingival crevicular fluids of 13 CP patients were collected at baseline, 2 weeks, 2 months and 4 months after periodontal mechanical treatment. Also, gingival crevicular fluids were collected from 11 healthy subjects. Six periodontal pathogenic bacteria including Actinobacillus actinomycetemcomitans (Aa), Porphyromonas gingivalis(Pg), Tannerella forsythensis (Tf), Prevotella intermedia (Pi), Fusobacterium nucleatum(Fn), Prevotella nigrescens (Pn) were detected by 16S rRNA based PCR.
  
  
  RESULTS
  The PLI, PD, BI of the CP patients 2 months and 4 months after periodontal mechanical treatment were evidently less than those before treatment. These 4 months after treatment were a little more than those 2 months after. The six bacteria were more frequently detected in the CP patients at baseline than in healthy controls. The prevalent rates of Tf (42.1%, 73.7%, 70.2%), Pg (47.4%, 68.4%, 77.2%), Aa (15.8%, 22.8%, 7.0%), Pn (38.6%, 57.9%, 64.9%), Pi(15.8%, 38.6%, 42.1%) 2 weeks, 2 months and 4 months following treatment were significantly lower than those at baseline (Tf 96.5%, Pg 93.0%, Aa 36.8%, Pn 86.0%, Pi 84.2%), but the prevalent rates of all the detected bacteria 2 months after treatment were higher than those at 2 weeks after.
  
  
  CONCLUSIONS
  Tf, Pg, Aa, Pn and Pi may cooperate in the development of CP. The changes of periodontal pathogenic bacteria could be detected before the changes of clinical parameters and the patients should be re-evaluated and re-treated regularly within 2 months after treatment.
authors.affiliation: Department of Stomatology, Beijing Anzhen Hospital of the Capital University of Medical Sciences, Beijing 100029, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ding
authors.forename: Huan-xin
authors.initials: HX
authors.lastname: Meng
authors.forename: Qi-qiang
authors.initials: QQ
authors.lastname: Li
authors.forename: Yi-bing
authors.initials: YB
authors.lastname: Zhao
authors.forename: Xiang-hui
authors.initials: XH
authors.lastname: Feng
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D055113:Chronic Periodontitis / Q000382:microbiology* / Q000628:therapy*; D005260:Female; D005883:Gingival Crevicular Fluid / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*; D045242:Prevotella nigrescens / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S
chemical_list: D012336:RNA, Ribosomal, 16S