Passiflora incarnata L. (Passionflower) extracts elicit GABA currents in hippocampal neurons in vitro, and show anxiogenic and anticonvulsant effects in vivo, varying with extraction method.
Potential mechanisms of Passiflora incarnata extracts and the effect of extraction methods on ingredients and biological effects were explored. Using the same batch of plant material, total flavonoid yields as measured by high-performance liquid chromatography coupled to diode array detection (HPLC-DAD) increased substantially with hot versus cold extraction methods. Whole Passiflora extract induced prominent, dose-dependent direct GABA(A) currents in hippocampal slices, but the expected modulation of synaptic GABA(A) currents was not seen. GABA was found to be a prominent ingredient of Passiflora extract, and GABA currents were absent when amino acids were removed from the extract. Five different extracts, prepared from a single batch of Passiflora incarnata, were administered to CF-1 mice for 1 week in their drinking water prior to evaluation of their behavioral effects. Anticonvulsant effects against PTZ-induced seizures were seen in mice that received 2 of the 5 Passiflora extracts. Instead of the anxiolytic effects described by others, anxiogenic effects in the elevated plus maze were seen in mice receiving any of the 5 Passiflora extracts.
Elsas
S-M
SM
Department of Neurology, Oregon Health and Science University, Portland, OR 97239, USA. elsas@ohsu.edu
Rossi
D J
DJ
Raber
J
J
White
G
G
Seeley
C-A
CA
Gregory
W L
WL
Mohr
C
C
Pfankuch
T
T
Soumyanath
A
A
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology / Q000627:therapeutic use*; D001007:Anxiety / Q000139:chemically induced / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D018755:GABA Agonists / Q000494:pharmacology / Q000627:therapeutic use*; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D008297:Male; D018782:Maze Learning; D051379:Mice; D008815:Mice, Inbred Strains; D029598:Passiflora / Q000737:chemistry*; D010433:Pentylenetetrazole; D008517:Phytotherapy; D010936:Plant Extracts / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000188:drug therapy*; D013696:Temperature; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology / Q000627:therapeutic use
D000927:Anticonvulsants; D005419:Flavonoids; D018755:GABA Agonists; D010936:Plant Extracts; D005680:gamma-Aminobutyric Acid; D010433:Pentylenetetrazole
J Clin Pharm Ther. 2001 Oct;26(5):369-73
11679027
J Food Sci. 2008 Jun;73(5):C323-8
18576976
Pharmacol Biochem Behav. 2004 Feb;77(2):399-404
14751470
J Ethnopharmacol. 1997 Jun;57(1):11-20
9234160
J Med Food. 2001 Autumn;4(3):137-144
12639407
Nature. 2000 Jan 20;403(6767):316-21
10659851
J Psychopharmacol. 2007 Jun;21(4):384-91
17092983
J Ethnopharmacol. 2004 Sep;94(1):1-23
15261959
J Pharm Biomed Anal. 2002 Oct 15;30(3):613-24
12367686
Planta Med. 1988 Dec;54(6):488-91
3212072
Biochem Pharmacol. 2004 Oct 15;68(8):1631-8
15451406
Epilepsy Behav. 2001 Dec;2(6):524-532
12609386
Neuropsychopharmacology. 2007 Mar;32(3):665-72
16710318
Pharmacol Biochem Behav. 2003 Jun;75(3):537-45
12895671
J Pharm Pharm Sci. 2003 May-Aug;6(2):215-22
12935433
Eur J Pharmacol. 2003 Feb 14;461(2-3):79-87
12586201
Anesth Analg. 2008 Jun;106(6):1728-32
18499602
Neurochem Res. 1997 Apr;22(4):419-25
9130252
Adv Pharmacol. 2006;54:285-316
17175819
J Ethnopharmacol. 2001 Dec;78(2-3):165-70
11694362
Curr Top Med Chem. 2002 Aug;2(8):853-67
12171576
Phytother Res. 2001 Mar;15(2):162-4
11268119
Planta Med. 2008 Dec;74(15):1769-73
19006051
Curr Opin Pharmacol. 2006 Feb;6(1):18-23
16376150
J Altern Complement Med. 2002 Jun;8(3):283-91
12165186
Biochem Pharmacol. 1990 Nov 15;40(10):2227-31
2173925
Prog Neuropsychopharmacol Biol Psychiatry. 2008 May 15;32(4):989-93
18262708
BMC Complement Altern Med. 2007 Aug 08;7:26
17686156
Exp Biol Med (Maywood). 2009 Aug;234(8):967-75
19491371
J Cereb Blood Flow Metab. 2001 Oct;21(10):1232-9
11598501
Phytother Res. 2006 May;20(5):348-51
16619361
Fitoterapia. 2000 Aug;71 Suppl 1:S117-23
10930722
Pharmacol Biochem Behav. 2003 Jun;75(3):501-12
12895668
J Clin Pharm Ther. 2001 Oct;26(5):363-7
11679026
Biochem Pharmacol. 2005 Apr 1;69(7):1101-11
15763546
Fitoterapia. 2001 Dec;72(8):922-6
11731118
Br J Pharmacol. 2004 Jul;142(5):811-20
15231642