title: Comparison of red blood cell membrane microstructure after different physicochemical influences: atomic force microscope research.
abstract: OBJECTIVE
  After the influence of different actions on the blood, the erythrocytes may change their macrostructure. At the same time, the microstructure of cell membrane will be changed as well. This study provides the results of comparison of red blood cell membrane microstructure after they have been affected by different factors.
  
  
  METHODS
  Images and spatial profiles of the cell surface were obtained by atomic force microscope. It was proposed to use spatial Fourier transform to decompose the initial complex profile into series of simple ones. This made it possible to compare surface parameters after exposure of red blood cells to different external actions.
  
  
  RESULTS
  Quantitative differences between membrane profile harmonic composition parameters (amplitude and spatial period) after physical impact (impulse electrical field, osmotic swelling) and after chemical impact (the fixing fluid glutaraldehyde and the drug Esmeron) were experimentally confirmed.
  
  
  CONCLUSIONS
  Such experimental and theoretical approach may lay down the foundations of mechanisms of different factors' effect on red blood cells both in research and in clinics.
authors.affiliation: V.A. Negovsky Research Institute of General Reanimatology, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Victor V
authors.initials: VV
authors.lastname: Moroz
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Chernysh
authors.forename: Elena K
authors.initials: EK
authors.lastname: Kozlova
authors.forename: Polina Yu
authors.initials: PY
authors.lastname: Borshegovskaya
authors.forename: Ulyana A
authors.initials: UA
authors.lastname: Bliznjuk
authors.forename: Regina M
authors.initials: RM
authors.lastname: Rysaeva
authors.forename: Olga Ye
authors.initials: OY
authors.lastname: Gudkova
mesh_terms: D000328:Adult; D000732:Androstanols / Q000494:pharmacology; D004560:Electricity; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000648:ultrastructure*; D005404:Fixatives / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D003473:Neuromuscular Nondepolarizing Agents / Q000494:pharmacology; D009995:Osmosis; D000077123:Rocuronium; D013499:Surface Properties
chemical_list: D000732:Androstanols; D005404:Fixatives; D003473:Neuromuscular Nondepolarizing Agents; D005976:Glutaral; D000077123:Rocuronium