p38 MAPK regulates Th2 cytokines release in PBMCs in allergic rhinitis rats.
Th2 cytokines play a pivotal role in the pathogenesis of allergic rhinitis. To investigate the effect of p38 mitogen-activated protein kinase (MAPK) on the production of Th2 cytokines such as IL-4 and IL-5 in allergic rhinitis, a model of allergic rhinitis was established in SD rats. The expression level of p38 MAPK mRNA in PBMCs was detected by means of real time quantitative RT-PCR. The p38 MAPK activity in PBMCs was detected by Western blotting. PBMCs were cultured with various concentrations of p38 MAPK inhibitor SB 239063 or without the treatment, and then IL-4, IL-5 levels of the supernatant were determined by using sandwich ELISA. The results showed that mRNA expression and activity of p38 MAPK in PBMCs were significantly higher in allergic rhinitis rats than in control rats (P<0.05). The p38 MAPK inhibitor SB 239063 decreased the production of IL-4 and IL-5 in a dose-dependent manner. It is concluded that p38 MAPK plays an important role in the pathogenesis of allergic rhinitis which is associated with Th2 cytokines release.
Liu
Jie
J
Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. LJ0901@163.com
Liu
Lisi
L
Cui
Yonghua
Y
Zhang
Jian
J
Jiang
Hongqun
H
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D007093:Imidazoles / Q000494:pharmacology; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D010047:Ovalbumin; D011743:Pyrimidines / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D006255:Rhinitis, Allergic, Seasonal / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*
D016207:Cytokines; D007093:Imidazoles; D015848:Interleukin-5; D011743:Pyrimidines; D012333:RNA, Messenger; D015847:Interleukin-4; D010047:Ovalbumin; D048051:p38 Mitogen-Activated Protein Kinases; C406525:SB 239063
Arch Bronconeumol. 2005 Oct;41(10):569-78
16266671
Eur J Immunol. 2005 Dec;35(12):3631-42
16259005
J Immunol. 1999 Nov 1;163(9):4763-71
10528175
Am J Respir Crit Care Med. 2005 Mar 15;171(6):571-8
15557129
Prim Care. 2008 Mar;35(1):1-10, v
18206714
Chest. 1997 Feb;111(2 Suppl):11S-16S
9042022
Arthritis Res Ther. 2006;8(2):205
16542479
Nat Rev Immunol. 2007 Mar;7(3):202-12
17318231
Methods. 2001 Dec;25(4):402-8
11846609
J Pharmacol Exp Ther. 2000 Apr;293(1):281-8
10734180
Am J Physiol. 1998 Sep;275(3):C623-33
9730944
J Allergy Clin Immunol. 2002 Apr;109(4):649-57
11941315
Biochim Biophys Acta. 2004 Mar 11;1697(1-2):53-69
15023350
Cell Immunol. 1999 Mar 15;192(2):87-95
10087176