title: 3,3',4,5,5'-Pentahydroxy-trans-stilbene, a resveratrol derivative, induces apoptosis in colorectal carcinoma cells via oxidative stress.
abstract: Resveratrol exhibits anti-tumor properties against different types of cancer. In this study, several polyhydroxylated resveratrol derivatives were prepared with the aim of discovering new leading compounds with clinical potential for human colon cancer chemotherapy. Among these compounds, 3,3',4,5,5'-pentahydroxy-trans-stilbene (PHS) displayed the most potent cytotoxicity and triggered apoptosis in HT-29 cells as evidenced by increased poly(ADP-ribose) polymerase (PARP) cleavage, elevated levels of cytoplasmic nucleosomes and DNA fragmentation. Further mechanistic analysis revealed that PHS-induced apoptosis was caspase-dependent and mediated by its pro-oxidative action through up-regulation of reactive oxidative species generation and depletion of intracellular glutathione.
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.forename: William Ka Kei
authors.initials: WK
authors.lastname: Wu
authors.forename: Zongping
authors.initials: Z
authors.lastname: Zheng
authors.forename: Chun Tao
authors.initials: CT
authors.lastname: Che
authors.forename: Zhi Jie
authors.initials: ZJ
authors.lastname: Li
authors.forename: Dan Dan
authors.initials: DD
authors.lastname: Xu
authors.forename: Clover Ching Man
authors.initials: CC
authors.lastname: Wong
authors.forename: Cai Guo
authors.initials: CG
authors.lastname: Ye
authors.forename: Joseph Jao Yiu
authors.initials: JJ
authors.lastname: Sung
authors.forename: Chi Hin
authors.initials: CH
authors.lastname: Cho
authors.forename: Mingfu
authors.initials: M
authors.lastname: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D003593:Cytoplasm / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005978:Glutathione / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D009707:Nucleosomes / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry* / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C473468:3,5,3',4',5'-pentahydroxy-stilbene; D000970:Antineoplastic Agents; D009707:Nucleosomes; D013267:Stilbenes; D011065:Poly(ADP-ribose) Polymerases; D005978:Glutathione; D000077185:Resveratrol