Mucin 21/epiglycanin modulates cell adhesion.
The molecular structure of mouse Mucin 21 (Muc21)/epiglycanin is proposed to have 98 tandem repeats of 15 amino acids and three exceptional repeats with 12 or 13 amino acids each, followed by a stem domain, a transmembrane domain, and a cytoplasmic tail. A cDNA of Muc21 having 84 tandem repeats of 15 amino acids was constructed and transfected using a Venus vector into HEK 293T cells. The fluorescent cells, which were considered to express Muc21, were nonadherent. This antiadhesion effect was lessened when constructs with smaller numbers of tandem repeats were used, suggesting that the tandem repeat domain plays a crucial role. Cells expressing Muc21 were significantly less adherent to each other and to extracellular matrix components than control cells. Antibody binding to the cell surface integrin subunits alpha5, alpha6, and beta1 was reduced in Muc21 transfectants in a tandem repeat-dependent manner, whereas equal amounts of proteins were detected by Western blot analysis. Muc21 was expressed as a large glycoprotein that was highly glycosylated with O-glycans at the cell surface, as detected by flow cytometry, Western blotting, and lectin blotting. Although at least a portion of Muc21 was glycosylated with sialylated glycans, removal of sialic acid did not influence the prevention of adhesion.
Yi
Yuri
Y
Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
Kamata-Sakurai
Mika
M
Denda-Nagai
Kaori
K
Itoh
Tomoko
T
Okada
Kyoko
K
Ishii-Schrade
Katrin
K
Iguchi
Akihiro
A
Sugiura
Daisuke
D
Irimura
Tatsuro
T
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D009077:Mucins / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry; D017434:Protein Structure, Tertiary
D000596:Amino Acids; D006023:Glycoproteins; C527666:MUC21 protein, human; D008562:Membrane Glycoproteins; D009077:Mucins; D011134:Polysaccharides; C016424:epiglycanin protein, mouse
Biol Reprod. 2008 Jan;78(1):134-42
17942799
Mol Biol Cell. 2000 Sep;11(9):3219-32
10982412
Glycobiology. 2008 Jan;18(1):74-83
17977904
Biol Reprod. 2000 Jul;63(1):294-300
10859271
Cancer Res. 1999 May 1;59(9):2229-36
10232613
Carbohydr Res. 1975 Mar;40(1):171-82
1125949
Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5001-5
1594606
Cancer Res. 1991 Feb 15;51(4):1170-6
1825477
Cancer Res. 1992 Apr 15;52(8):2318-24
1559234
J Natl Cancer Inst. 1972 May;48(5):1463-76
5030958
J Cell Biol. 1995 Apr;129(1):255-65
7698991
Glycobiology. 1992 Jun;2(3):173-80
1498415
Eur J Cancer. 2009 Jan;45(1):164-73
19046630
Biochemistry. 1972 Jul 4;11(14):2559-64
5065218
J Natl Cancer Inst. 1971 Aug;47(2):343-59
4934423
J Natl Cancer Inst. 1979 Jul;63(1):153-61
286825
J Biol Chem. 1997 Dec 26;272(52):33245-54
9407114
J Cell Biol. 1977 Mar;72(3):511-29
838766
J Natl Cancer Inst. 1982 Jun;68(6):981-8
6953278
Cancer Res. 2005 Jan 15;65(2):473-82
15695389
J Natl Cancer Inst. 1979 Jul;63(1):163-9
221714
J Cell Biol. 1994 Dec;127(6 Pt 2):2071-80
7528749
Mol Biol Cell. 1996 Apr;7(4):565-77
8730100