Anticandidal activity of cinnamaldehyde, its ligand and Ni(II) complex: effect of increase in ring and side chain.
To increase efficacy of cinnamaldehyde as an antimycotic agent, N, N'- Bis (trans-cinnamadehyde) ethylenediimine [C(20)H(20)N(2)] and Ni(II) complex of the type [Ni(C(40)H(40)N(4))Cl(2)] have been synthesized. The ligand [P] and Ni(II) complex have been characterized on the basis of elemental analysis, FTIR, ESI- MS, IR, (1)H NMR, UV-Vis spectroscopic techniques, conductivity and magnetic measurements. MIC of cinnamaldehyde against clinical isolate of Candida albicans and Candida tropicalis was 400 microg/ml and 500 microg/ml, respectively. Synthesized ligand has markedly reduced MIC; 200 microg/ml and 300 microg/ml whereas Ni(II) complex of ligand displayed MIC of 90 microg/ml and 120 microg/ml. Growth and sensitivity of the organisms were effected by ligand & complex at significantly reduced concentration. Plasma membrane ATPase activity and ergosterol content have been investigated as site of action. Result obtained indicates ergosterol biosynthesis pathway as site of action of cinnamaldehyde, synthesized ligand and its Ni(II) complex.
Shreaz
Sheikh
S
Department of Biosciences, Jamia Millia Islamia (Central University), New Delhi, India.
Sheikh
Rayees A
RA
Rimple
Bhatia
B
Hashmi
Athar Adil
AA
Nikhat
Manzoor
M
Khan
Luqman A
LA
D000171:Acrolein / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000201:enzymology; D041022:Candida tropicalis / Q000187:drug effects* / Q000201:enzymology; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology; D004553:Electric Conductivity; D004875:Ergosterol / Q000032:analysis; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics; D008826:Microbial Sensitivity Tests; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
D000935:Antifungal Agents; D000171:Acrolein; D000251:Adenosine Triphosphatases; C012843:cinnamaldehyde; D004875:Ergosterol