title: Development and evaluation of porcine cysticercosis QuickELISA in Triturus EIA analyzer.
abstract: We evaluated three diagnostic antigens (recombinant GP50, recombinant T24H, and synthetic Ts18var1) for cysticercosis and found that all three performed well in detecting cysticercosis in humans and pigs in several assay formats. These antigens were adapted to a new antibody detection format (QuickELISA). With one single incubation step which involves all reactants except the enzyme substrate, the QuickELISA is particularly suited for automation. We formatted the QuickELISA for the Triturus EIA analyzer for testing large numbers of samples. We found that in QuickELISA formats rGP50 and rT24H have better sensitivity and specificity than sTs18var1 for detecting porcine cysticercosis.
authors.affiliation: Atlanta Research and Education Foundation, Atlanta, Georgia, USA. ahi0@cdc.gov
authors.forename: Sukwan
authors.initials: S
authors.lastname: Handali
authors.forename: Sowmya
authors.initials: S
authors.lastname: Pattabhi
authors.forename: Yeuk-Mui
authors.initials: YM
authors.lastname: Lee
authors.forename: Maria
authors.initials: M
authors.lastname: Silva-Ibanez
authors.forename: Victor A
authors.initials: VA
authors.lastname: Kovalenko
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Levin
authors.forename: Armando E
authors.initials: AE
authors.lastname: Gonzalez
authors.forename: Jacquelin M
authors.initials: JM
authors.lastname: Roberts
authors.forename: Hector H
authors.initials: HH
authors.lastname: Garcia
authors.forename: Robert H
authors.initials: RH
authors.lastname: Gilman
authors.forename: Kathy
authors.initials: K
authors.lastname: Hancock
authors.forename: Victor C W
authors.initials: VC
authors.lastname: Tsang
mesh_terms: D000818:Animals; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*