Systemic delivery of synthetic siRNAs.
RNA interference is a biological process for gene silencing that can be harnessed for the development of new drugs. However, a major obstacle to the use of small interfering RNAs (siRNAs) as therapeutics is their delivery across the plasma membrane of cells in vivo. A range of solutions for this challenge have been described, including cationic lipids, high-pressure injection, viral vectors, and chemical modifications of the siRNAs. This chapter describes cationic lipid delivery of siRNAs to adult mice.
Sørensen
Dag R
DR
Department of Comparative Medicine, Rikshospitalet-Radiumhospitalet, University Hospital, Oslo, Norway.
Sioud
Mouldy
M
D000818:Animals; D002448:Cell Adhesion; D002469:Cell Separation; D018014:Gene Transfer Techniques; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000378:metabolism; D051379:Mice; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000378:metabolism; D014162:Transfection
D034741:RNA, Small Interfering