Protein electroporation into Chlamydomonas for mutant rescue.
Electroporation can be used to deliver proteins into Chlamydomonas cells. This technique is particularly useful to demonstrate rescue of paralyzed-flagella mutants with recombinant proteins that complement the mutations. The appearance of motile cells immediately provides assurance that the recombinant proteins have their native structures and are functional. It can be also used for the introduction of artificially modified proteins or other substances into live cells.
Hayashi
Masahito
M
Department of Biological Sciences, University of Tokyo, Bunkyo-ku, Japan.
Kamiya
Ritsu
R
D020418:Algal Proteins / Q000378:metabolism*; D002696:Chlamydomonas / Q000378:metabolism*; D004560:Electricity; D018274:Electroporation / Q000379:methods*; D005816:Genetic Complementation Test / Q000379:methods*; D009154:Mutation / Q000235:genetics*
D020418:Algal Proteins