Modification of mouse nodal flow by applying artificial flow.
In mammalian development, the earliest left-right (L-R) asymmetry is nodal flow, which is a cilia-driven leftward fluid flow on the ventral surface of the node. The importance of nodal flow for L-R determination was demonstrated by experiments to modify nodal flow by imposing artificial fluid flow. In this system, cultured mouse embryos developed reversed L-R asymmetry when their node cavity had rightward flow, and normal L-R asymmetry when their node had leftward flow. This chapter describes details of the culture system that can modify nodal flow.
Nonaka
Shigenori
S
Laboratory for Spatiotemporal Regulations, National Institute for Basic Biology, Nishigonaka 38, Myodaiji, Okazaki 444-8585 Aichi, Japan.
D000818:Animals; D019521:Body Patterning; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002923:Cilia / Q000378:metabolism / Q000648:ultrastructure; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D051379:Mice