Analysis of floral transcription factors from Lycoris longituba.
Transcription factors (TFs) are proteins that bind to specific promoter regions of their target genes and regulate gene transcription. Many of these factors have been found to influence flowering. Lycoris longituba exhibits a great deal of diversity in flower color and flower form, making it a suitable model for the study of floral development. We have identified 338 putative TFs from more than thirty thousand ESTs sequenced from the floral tissue of L. longituba, and validated them using real-time RT-PCR. Fifty-one of the TFs were recognized as being potentially flower-specific, and the expression patterns of some of them during six flowering phases have been elucidated. Homolog annotation and phylogenetic analysis revealed that some TFs that belong to several TF families, such as MADS, MYB-related, NAC, and ABI3-VP1, were suggested to play important roles in the flowering process. Our dataset may be used to identify priority target TF genes for further study.
He
Qiu-Ling
QL
Nanjing Forestry University, China.
Cui
Shao-Jie
SJ
Gu
Jian-Lei
JL
Zhang
He
H
Wang
Ming-Xiu
MX
Zhou
Yan
Y
Zhang
Liang
L
Huang
Min-Ren
MR
D001483:Base Sequence; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D031426:Lycoris / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
D018076:DNA, Complementary; D014157:Transcription Factors