Analysis of redox-sensitive dynein components.
Redox-based regulation plays important roles in many cellular activities. Thioredoxins, one of the best characterized class of proteins involved in cellular redox regulation, are conserved components of eukaryotic ciliary/flagellar axonemal dyneins. Studies with Chlamydomonas showed that, under varying redox conditions, dynein-associated thioredoxins interact with different proteins through disulfide bonds and, as a consequence, flagella change their manner of beating. This chapter provides an overview of techniques for estimating and modulating the redox state of axonemal proteins, as well as for searching for redox-regulated proteins in the axoneme.
Wakabayashi
Ken-ichi
K
Department of Biological Sciences, The University of Tokyo, Japan.
D020418:Algal Proteins / Q000302:isolation & purification / Q000378:metabolism; D054468:Axoneme / Q000378:metabolism; D001671:Biochemistry / Q000379:methods*; D002021:Buffers; D002696:Chlamydomonas / Q000378:metabolism; D004398:Dyneins / Q000302:isolation & purification / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005407:Flagella / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010084:Oxidation-Reduction; D021122:Protein Subunits / Q000378:metabolism; D013267:Stilbenes / Q000378:metabolism; D013451:Sulfonic Acids / Q000378:metabolism
D020418:Algal Proteins; D002021:Buffers; D021122:Protein Subunits; D013267:Stilbenes; D013451:Sulfonic Acids; C079532:4-acetamido-4'-maleimidylstilbene-2,2'-disulfonic acid; D004398:Dyneins