Critical function of death-associated protein 3 in T cell receptor-mediated apoptosis induction.
Death-associated protein 3 (DAP3) is crucial for promoting apoptosis induced by various stimulations. This report demonstrates that DAP3 is also important for T cell receptor (TCR)-mediated apoptosis induction in immature thymocytes. Enforced expression of DAP3 accelerated the negative selection in developing thymocytes, using the reaggregate thymus organ culture system. In addition, expression of DAP3 accelerated TCR-mediated apoptosis induction in DO11.10 cells. We also demonstrated that DAP3 translocates into the nucleus during TCR-mediated apoptosis in a Nur77 dependent manner. It is concluded that DAP3 is critical for TCR-mediated induction of apoptosis at the downstream of Nur77.
Tosa
Noriko
N
Institute for Animal Experimentation, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Japan.
Iwai
Atsushi
A
Tanaka
Taku
T
Kumagai
Tomoka
T
Nitta
Takeshi
T
Chiba
Satoko
S
Maeda
Masahiro
M
Takahama
Yousuke
Y
Uede
Toshimitsu
T
Miyazaki
Tadaaki
T
D021581:Active Transport, Cell Nucleus; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D002467:Cell Nucleus / Q000378:metabolism; D051379:Mice; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1 / Q000502:physiology; D011506:Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016601:RNA-Binding Proteins; D011948:Receptors, Antigen, T-Cell / Q000819:agonists / Q000502:physiology*
D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C490762:Dap3 protein, mouse; C539818:Nr4a1 protein, mouse; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D011506:Proteins; D016601:RNA-Binding Proteins; D011948:Receptors, Antigen, T-Cell