title: Expression of podoplanin in human bone and bone tumors: New marker of osteogenic and chondrogenic bone tumors.
abstract: Podoplanin is known as a lymphatic marker because its expression is detected in lymphatic but not vascular endothelium. Podoplanin is also expressed in several normal tissues including osteocytes or osteoblasts. A systematic examination of the podoplanin expression was conducted in normal skeletal tissues and some bone tumor cell lines, and the diagnostic value determined in primary bone tumors. Podoplanin mRNA was expressed at a high level in bone marrow tissue and cartilage, and was upregulated with differentiation to osteoblasts in bone marrow cells. Strong podoplanin expression was seen in osteocytes, chondrocytes, and osteoblasts on immunohistochemistry. Podoplanin mRNA was expressed at a high level in several osteosarcoma and chondrosarcoma cell lines, whereas podoplanin was expressed at a low level in a Ewing's/primitive neuroectodermal tumor cell line. In the clinical samples, osteosarcomas (22/26) expressed podoplanin at various levels. In small cell osteosarcomas (2/2), podoplanin was expressed strongly, although the tissue samples included few diagnostic osteoids. Chondrosarcomas (10/10) expressed podoplanin strongly, and chondroblastomas (5/5) expressed podoplanin moderately, while podoplanin was absent or expressed at low levels in Ewing's sarcomas (0/5), chordomas (0/6) and giant cell tumors of bone (1/7). Therefore, podoplanin may be a sensitive immunohistochemical marker of osteogenic and chondrogenic bone tumors.
authors.affiliation: Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 757-1, Asahimachi-dori, Niigata City, Niigata 951-8510, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ariizumi
authors.forename: Akira
authors.initials: A
authors.lastname: Ogose
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawashima
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Hotta
authors.forename: Guidong
authors.initials: G
authors.lastname: Li
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Xu
authors.forename: Hajime
authors.initials: H
authors.lastname: Umezu
authors.forename: Mika
authors.initials: M
authors.lastname: Sugai
authors.forename: Naoto
authors.initials: N
authors.lastname: Endo
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism*; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D002804:Chondroblastoma / Q000235:genetics / Q000378:metabolism; D002813:Chondrosarcoma / Q000235:genetics / Q000378:metabolism*; D005870:Giant Cell Tumors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012512:Sarcoma, Ewing / Q000235:genetics / Q000378:metabolism; D018228:Sarcoma, Small Cell / Q000235:genetics / Q000378:metabolism; D000098853:Podoplanin
chemical_list: D014408:Biomarkers, Tumor; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; C117468:PDPN protein, human; D000098853:Podoplanin