title: Calmodulin-binding transcription activator 1 mediates auxin signaling and responds to stresses in Arabidopsis.
abstract: Auxin is a key plant hormone that regulates various aspects of plant development. However, the mechanisms integrating auxin growth effects with stress responses are not fully understood. In this study, we investigated the possible role of calmodulin-binding transcription activator 1 (CAMTA1), an Arabidopsis thaliana calcium/calmodulin-binding transcription activator, in auxin signaling and its responses to different stresses. Plants harboring the AtCAMTA1 promoter fused to the GUS reporter gene revealed cell-specific expression patterns reminiscent of auxin responses. The responsiveness of CAMTA1 to auxin was further assessed by chemical disturbances in polar auxin transport, and by RT-PCR analysis of gene expression of dissected leaf sections from plants exposed to the auxin transport inhibitor NPA. Furthermore, the intensity and cell-specific expression patterns of CAMTA1 changed significantly and differentially on exposure to increasing salt concentrations and heat. Transcriptome analysis of a camta1 T-DNA insertion mutant revealed 63 up-regulated genes, of which 17 are associated with auxin signaling. Finally, analysis of hypocotyl elongation in the presence and absence of auxin revealed that camta1 T-DNA insertion mutants and CAMTA1-repressor lines are hyper-responsive to auxin compared to wild-type seedlings. Thus, CAMTA1 participates in auxin signaling and responds to abiotic stresses.
authors.affiliation: Department of Plant Sciences, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel. galonyae@post.tau.ac.il
authors.forename: Yael
authors.initials: Y
authors.lastname: Galon
authors.forename: Roni
authors.initials: R
authors.lastname: Aloni
authors.forename: Dikla
authors.initials: D
authors.lastname: Nachmias
authors.forename: Orli
authors.initials: O
authors.lastname: Snir
authors.forename: Ester
authors.initials: E
authors.lastname: Feldmesser
authors.forename: Sarah
authors.initials: S
authors.lastname: Scrase-Field
authors.forename: Joy M
authors.initials: JM
authors.lastname: Boyce
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bouché
authors.forename: Marc R
authors.initials: MR
authors.lastname: Knight
authors.forename: Hillel
authors.initials: H
authors.lastname: Fromm
mesh_terms: D017360:Arabidopsis / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D013312:Stress, Physiological
chemical_list: D002135:Calcium-Binding Proteins; D017931:DNA Primers; D007210:Indoleacetic Acids
references.citation: Plant Cell. 1997 Nov;9(11):1963-71
references.pmid: 9401121
references.citation: Biostatistics. 2003 Apr;4(2):249-64
references.pmid: 12925520
references.citation: J Exp Bot. 2006;57(15):4059-70
references.pmid: 17077182
references.citation: Plant J. 2008 Aug;55(3):526-42
references.pmid: 18419781
references.citation: Cell. 2006 Nov 17;127(4):847-58
references.pmid: 17110341
references.citation: Plant J. 2005 Dec;44(6):903-16
references.pmid: 16359384
references.citation: Plant Mol Biol. 2005 Nov;59(5):821-38
references.pmid: 16270233
references.citation: J Biol Chem. 2002 Nov 22;277(47):45049-58
references.pmid: 12218065
references.citation: Nature. 2005 Jan 6;433(7021):39-44
references.pmid: 15635403
references.citation: Plant Cell Physiol. 2006 Jun;47(6):788-92
references.pmid: 16621846
references.citation: J Biol Chem. 2000 Feb 4;275(5):3137-43
references.pmid: 10652297
references.citation: Plant Cell. 2007 Aug;19(8):2440-53
references.pmid: 17675404
references.citation: Plant Cell. 2001 Jul;13(7):1499-510
references.pmid: 11449047
references.citation: Plant Cell. 2008 Apr;20(4):947-68
references.pmid: 18424613
references.citation: FEBS Lett. 2008 Mar 19;582(6):943-8
references.pmid: 18298954
references.citation: Curr Opin Plant Biol. 2007 Oct;10(5):453-60
references.pmid: 17900969
references.citation: Nature. 2009 Feb 26;457(7233):1154-8
references.pmid: 19122675
references.citation: Plant Physiol. 2006 Sep;142(1):113-23
references.pmid: 16844836
references.citation: Plant Physiol. 1999 Dec;121(4):1179-90
references.pmid: 10594105
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: J Biol Chem. 2002 Jun 14;277(24):21851-61
references.pmid: 11925432
references.citation: Plant J. 2000 Nov;24(4):533-42
references.pmid: 11115134
references.citation: Plant Cell. 2009 Mar;21(3):972-84
references.pmid: 19270186
references.citation: Genetics. 1994 Dec;138(4):1239-49
references.pmid: 7896103
references.citation: Plant Cell Physiol. 2003 Oct;44(10):975-81
references.pmid: 14581622
references.citation: Curr Opin Plant Biol. 2007 Aug;10(4):366-71
references.pmid: 17644023
references.citation: Plant Physiol. 2002 Jun;129(2):661-77
references.pmid: 12068110
references.citation: Plant Cell Physiol. 2008 Oct;49(10):1429-50
references.pmid: 18718932
references.citation: FEBS Lett. 2007 Aug 21;581(21):3893-8
references.pmid: 17689537
references.citation: Plant Physiol. 1998 Feb;116(2):455-62
references.pmid: 9489005
references.citation: Plant Sci. 2001 Feb 5;160(3):381-404
references.pmid: 11166425
references.citation: Plant Cell Rep. 2008 Mar;27(3):575-84
references.pmid: 17926040
references.citation: Plant J. 2007 Apr;50(2):347-63
references.pmid: 17376166
references.citation: Curr Opin Plant Biol. 2003 Oct;6(5):500-6
references.pmid: 12972052
references.citation: Plant Physiol. 2008 Mar;146(3):1408-20
references.pmid: 18218969
references.citation: Science. 2005 Oct 7;310(5745):121-5
references.pmid: 16210544
references.citation: Planta. 2006 Jan;223(2):315-28
references.pmid: 16208486
references.citation: Plant Biol (Stuttg). 2006 May;8(3):353-9
references.pmid: 16807828
references.citation: Mol Plant Microbe Interact. 2007 Aug;20(8):900-11
references.pmid: 17722694
references.citation: Plant Mol Biol. 2007 Dec;65(6):763-73
references.pmid: 17922210
references.citation: J Exp Bot. 2007;58(8):2145-57
references.pmid: 17490995
references.citation: Plant J. 2003 Feb;33(4):633-50
references.pmid: 12609038
references.citation: Plant J. 2005 Jul;43(1):153-63
references.pmid: 15960624
references.citation: Mol Plant. 2009 Jan;2(1):13-21
references.pmid: 19529824
references.citation: Plant Cell. 2007 Jan;19(1):148-62
references.pmid: 17237352
references.citation: EMBO J. 1987 Dec 20;6(13):3901-7
references.pmid: 3327686
references.citation: Plant J. 2003 Jun;34(5):733-9
references.pmid: 12787253
references.citation: Plant Mol Biol. 2007 Jan;63(2):221-35
references.pmid: 17063378
references.citation: Biochem Biophys Res Commun. 2005 Apr 1;329(1):406-15
references.pmid: 15721321
references.citation: J Plant Physiol. 2004 Aug;161(8):937-45
references.pmid: 15384405
references.citation: J Chem Ecol. 2001 Feb;27(2):327-42
references.pmid: 14768818
references.citation: Dev Cell. 2005 Jul;9(1):109-19
references.pmid: 15992545
references.citation: Plant Physiol. 2003 Dec;133(4):1617-29
references.pmid: 14645734
references.citation: J Exp Bot. 2001 Feb;52(355):215-21
references.pmid: 11283165
references.citation: Planta. 2003 Mar;216(5):841-53
references.pmid: 12624772
references.citation: Science. 2006 Apr 21;312(5772):436-9
references.pmid: 16627744
references.citation: Annu Rev Cell Dev Biol. 2008;24:55-80
references.pmid: 18631113
references.citation: Plant Physiol. 2003 Jul;132(3):1623-30
references.pmid: 12857841