Acyl-ACP thioesterases from castor (Ricinus communis L.): an enzymatic system appropriate for high rates of oil synthesis and accumulation.
Acyl-acyl carrier protein (ACP) thioesterases are enzymes that terminate the intraplastidial fatty acid synthesis in plants by hydrolyzing the acyl-ACP intermediates and releasing free fatty acids to be incorporated into glycerolipids. These enzymes are classified in two families, FatA and FatB, which differ in amino acid sequence and substrate specificity. In the present work, both FatA and FatB thioesterases were cloned, sequenced and characterized from castor (Ricinus communis) seeds, a crop of high interest in oleochemistry. Single copies of FatA and FatB were found in castor resulting to be closely related with those of Jatropha curcas. The corresponding mature proteins were heterologously expressed in Escherichia coli for biochemical characterization after purification, resulting in high catalytic efficiency of RcFatA on oleoyl-ACP and palmitoleoyl-ACP and high efficiencies of RcFatB for oleoyl-ACP and palmitoyl-ACP. The expression profile of these genes displayed the highest levels in expanding tissues that typically are very active in lipid biosynthesis such as developing seed endosperm and young expanding leaves. The contribution of these two enzymes to the synthesis of castor oil is discussed.
Sánchez-García
Alicia
A
Instituto de la Grasa (CSIC), Seville, Spain.
Moreno-Pérez
Antonio J
AJ
Muro-Pastor
Alicia M
AM
Salas
Joaquín J
JJ
Garcés
Rafael
R
Martínez-Force
Enrique
E
D002367:Ricinus communis / Q000201:enzymology* / Q000378:metabolism; D002368:Castor Oil / Q000032:analysis / Q000096:biosynthesis; D008969:Molecular Sequence Data; D015394:Molecular Structure; D020539:Sequence Analysis, Protein; D012689:Sequence Homology, Nucleic Acid; D013869:Thiolester Hydrolases / Q000378:metabolism*
D002368:Castor Oil; D013869:Thiolester Hydrolases; C018748:oleoyl-(acyl-carrier-protein) hydrolase