title: Role of Streptococcus mutans eukaryotic-type serine/threonine protein kinase in interspecies interactions with Streptococcus sanguinis.
abstract: OBJECTIVE
  Interspecies interactions of oral streptococci involve the production and excretion of antimicrobial compounds to compete successfully during colonization. Bacteriocin production by Streptococcus mutans and hydrogen peroxide (H2O2) production by Streptococcus sanguinis have been demonstrated as crucial for the clinical relevant antagonism between both species. A potential target of H2O2 is the cell-envelop of S. mutans. In the present study, the role of cell-envelop associated eukaryotic serine/threonine protein kinase (STPK) in S. mutans during interspecies competition has been investigated.
  
  
  METHODS
  Allelic replacement via homologous recombination of the STPK encoding gene with a kanamycin resistant determinant has been constructed. The mutant has been screened for the susceptibility towards cell-envelope stress. A previously developed spotting assay was used to simulate interspecies competition.
  
  
  RESULTS
  The STPK(-) mutant showed an increased susceptibility towards envelop stress caused by H2O2 and was significantly more inhibited during interspecies competition assays.
  
  
  CONCLUSIONS
  S. mutans is able to sense antimicrobial compounds excreted by competing species and can potentially adjust the cell-envelop towards an increased resistance.
authors.affiliation: College of Dentistry, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.forename: Jens
authors.initials: J
authors.lastname: Kreth
mesh_terms: D000222:Adaptation, Physiological; D000898:Antibiosis; D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms / Q000254:growth & development; D002473:Cell Wall / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D013295:Streptococcus mutans / Q000201:enzymology* / Q000235:genetics; D013298:Streptococcus sanguis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D016877:Oxidants; D006861:Hydrogen Peroxide; D017346:Protein Serine-Threonine Kinases
references.citation: Science. 2006 Sep 15;313(5793):1636-7
references.pmid: 16973881
references.citation: Lett Appl Microbiol. 2008 Nov;47(5):439-44
references.pmid: 19146535
references.citation: Appl Environ Microbiol. 2005 Jan;71(1):354-62
references.pmid: 15640209
references.citation: Mol Microbiol. 2002 Oct;46(2):571-86
references.pmid: 12406230
references.citation: J Bacteriol. 2008 Jul;190(13):4632-40
references.pmid: 18441055
references.citation: Mol Microbiol. 2005 Jul;57(2):392-404
references.pmid: 15978073
references.citation: Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14434-9
references.pmid: 12397186
references.citation: J Bacteriol. 2007 Jun;189(11):4168-79
references.pmid: 17416671
references.citation: Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):10108-13
references.pmid: 12096194
references.citation: Anal Biochem. 1993 Oct;214(1):343-6
references.pmid: 8250246
references.citation: Trends Biochem Sci. 2002 Sep;27(9):438
references.pmid: 12217513
references.citation: Microbiology (Reading). 2009 Nov;155(Pt 11):3691-3700
references.pmid: 19684069
references.citation: BMC Genomics. 2005 Sep 19;6:129
references.pmid: 16171520
references.citation: J Bacteriol. 2007 Apr;189(8):3166-75
references.pmid: 17277061
references.citation: Can J Microbiol. 2008 Oct;54(10):868-75
references.pmid: 18923556
references.citation: Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3508-13
references.pmid: 17360674
references.citation: FEMS Microbiol Rev. 2008 Jan;32(1):107-46
references.pmid: 18173394
references.citation: J Bacteriol. 2006 Feb;188(4):1628-32
references.pmid: 16452447
references.citation: J Bacteriol. 2005 Nov;187(21):7193-203
references.pmid: 16237003
references.citation: Infect Immun. 2004 Apr;72(4):2434-7
references.pmid: 15039376
references.citation: Biochemistry. 2008 Mar 11;47(10):3308-14
references.pmid: 18266322
references.citation: Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Jul;35(4):555-8
references.pmid: 15291127
references.citation: Infect Immun. 2009 Apr;77(4):1406-16
references.pmid: 19188361
references.citation: Mol Microbiol. 2005 Aug;57(4):960-9
references.pmid: 16091037
references.citation: Appl Environ Microbiol. 2001 Jan;67(1):15-21
references.pmid: 11133423
references.citation: Infect Immun. 1983 Apr;40(1):70-80
references.pmid: 6832837
references.citation: FEBS J. 2005 Mar;272(5):1243-54
references.pmid: 15720398