title: Leptographium bhutanense sp. nov., associated with the root collar weevil Hylobitelus chenkupdorjii on Pinus wallichiana in Bhutan.
abstract: Leptographium spp. are commonly associated with bark beetles and weevils (Coleoptera: Curculionidae), and some are important tree pathogens. In a recent survey of diseases and insect pests of conifer trees in Bhutan, the root collar weevil, Hylobitelus chenkupdorjii was found girdling young Himalayan blue pine (Pinus wallichiana) trees in Central Bhutan. Intensive wood staining and a Leptographium sp. were associated with damage by this insect. The fungus was also isolated from individuals of H. chenkupdorjii. It was tentatively identified based on morphology and then compared with other Leptographium spp. using DNA sequences for three gene regions. Morphological characteristics showed that the Leptographium sp. from H. chenkupdorjii is similar to, but distinct from L. procerum and L. profanum. DNA sequence comparisons revealed that the isolates from Bhutan resided in a distinct well-supported clade and confirmed that they represent an undescribed taxon for which the name Leptographium bhutanense sp. nov. is provided.
authors.affiliation: Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa;
authors.forename: X D
authors.initials: XD
authors.lastname: Zhou
authors.forename: K
authors.initials: K
authors.lastname: Jacobs
authors.forename: T
authors.initials: T
authors.lastname: Kirisits
authors.forename: D B
authors.initials: DB
authors.lastname: Chhetri
authors.forename: M J
authors.initials: MJ
authors.lastname: Wingfield
references.citation: Mycol Res. 2004 Apr;108(Pt 4):411-8
references.pmid: 15209281
references.citation: Stud Mycol. 2006;55:75-97
references.pmid: 18490973
references.citation: Syst Biol. 2000 Dec;49(4):628-51
references.pmid: 12116431
references.citation: Appl Environ Microbiol. 1995 Apr;61(4):1323-30
references.pmid: 7747954
references.citation: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references.pmid: 9396791