Involvement of phospholipase D and phosphatidic acid in the light-dependent up-regulation of sorghum leaf phosphoenolpyruvate carboxylase-kinase.
The photosynthetic phosphoenolpyruvate carboxylase (C(4)-PEPC) is regulated by phosphorylation by a phosphoenolpyruvate carboxylase kinase (PEPC-k). In Digitaria sanguinalis mesophyll protoplasts, this light-mediated transduction cascade principally requires a phosphoinositide-specific phospholipase C (PI-PLC) and a Ca(2+)-dependent step. The present study investigates the cascade components at the higher integrated level of Sorghum bicolor leaf discs and leaves. PEPC-k up-regulation required light and photosynthetic electron transport. However, the PI-PLC inhibitor U-73122 and inhibitors of calcium release from intracellular stores only partially blocked this process. Analysis of [(32)P]phosphate-labelled phospholipids showed a light-dependent increase in phospholipase D (PLD) activity. Treatment of leaf discs with n-butanol, which decreases the formation of phosphatidic acid (PA) by PLD, led to the partial inhibition of the C(4)-PEPC phosphorylation, suggesting the participation of PLD/PA in the signalling cascade. PPCK1 gene expression was strictly light-dependent. Addition of neomycin or n-butanol decreased, and a combination of both inhibitors markedly reduced PPCK1 expression and the concomitant rise in PEPC-k activity. The calcium/calmodulin antagonist W7 blocked the light-dependent up-regulation of PEPC-k, pointing to a Ca(2+)-dependent protein kinase (CDPK) integrating both second messengers, calcium and PA, which were shown to increase the activity of sorghum CDPK.
Monreal
José Antonio
JA
Departamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Avenida Reina Mercedes no. 6, Seville, Spain.
López-Baena
Francisco Javier
FJ
Vidal
Jean
J
Echevarría
Cristina
C
García-Mauriño
Sofía
S
D020001:1-Butanol / Q000494:pharmacology; D004579:Electron Transport / Q000187:drug effects / Q000528:radiation effects; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D008027:Light; D055537:Light Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D010712:Phosphatidic Acids / Q000378:metabolism*; D010730:Phosphoenolpyruvate Carboxylase / Q000378:metabolism; D054801:Phosphoinositide Phospholipase C / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism*; D010788:Photosynthesis / Q000187:drug effects / Q000528:radiation effects; D018515:Plant Leaves / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D045868:Sorghum / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000528:radiation effects*
D004791:Enzyme Inhibitors; D010712:Phosphatidic Acids; D020001:1-Butanol; D011494:Protein Kinases; C069767:phosphoenolpyruvate carboxylase kinase; D017346:Protein Serine-Threonine Kinases; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D010739:Phospholipase D; D010730:Phosphoenolpyruvate Carboxylase
FEBS Lett. 1990 Nov 26;275(1-2):25-8
2148159
Plant Cell. 1995 Dec;7(12):2197-2210
12242371
Plant Cell. 1996 Apr;8(4):573-586
12239393
J Lipid Res. 2009 Apr;50 Suppl:S260-5
19098305
Plant J. 2000 Aug;23(4):497-506
10972876
Biochim Biophys Acta. 1989 Feb 13;979(1):105-12
2537103
Plant J. 2006 Oct;48(1):45-53
16925599
Curr Opin Plant Biol. 2006 Oct;9(5):515-22
16877031
Trends Plant Sci. 2001 May;6(5):227-33
11335176
Plant Physiol. 2004 Aug;135(4):2078-87
15299132
Plant Physiol. 2003 Dec;133(4):2021-8
14630963
Arch Biochem Biophys. 2003 Jun 15;414(2):189-96
12781770
FEBS Lett. 2007 Jul 24;581(18):3468-72
17618627
Trends Plant Sci. 2005 Aug;10(8):368-75
16023886
Subcell Biochem. 2006;39:207-37
17121277
Eur J Biochem. 1992 Mar 1;204(2):821-30
1311681
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4354-7
6945588
Anal Biochem. 1976 May 7;72:248-54
942051
Nature. 2009 Jan 29;457(7229):551-6
19189423
Plant J. 2006 Jul;47(2):258-68
16762031
Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:211-231
11337397
Acta Biochim Pol. 2007;54(2):219-33
17446936
Plant Physiol. 2005 Oct;139(2):566-73
16219918
Plant Physiol. 2005 Dec;139(4):1970-83
16299185
Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:273-298
15012290
Planta. 2007 Mar;225(4):801-12
16983537
Biochem J. 2007 Jul 1;405(1):191-8
17376028
Biochem Biophys Res Commun. 2004 Feb 6;314(2):428-33
14733923
Arch Biochem Biophys. 1994 Dec;315(2):425-30
7986087
Plant Physiol. 2009 May;150(1):424-36
19304931