title: A doppel alpha-helix peptide fragment mimics the copper(II) interactions with the whole protein.
abstract: The doppel protein (Dpl) is the first homologue of the prion protein (PrP(C)) to be discovered; it is overexpressed in transgenic mice that lack the prion gene, resulting in neurotoxicity. The whole prion protein is able to inhibit Dpl neurotoxicity, and its N-terminal domain is the determinant part of the protein function. This region represents the main copper(II) binding site of PrP(C). Dpl is able to bind at least one copper ion, and the specific metal-binding site has been identified as the histidine residue at the beginning of the third helical region. However, a reliable characterization of copper(II) coordination features has not been reported. In a previous paper, we studied the copper(II) interaction with a peptide that encompasses only the loop region potentially involved in metal binding. Nevertheless, we did not find a complete match between the EPR spectroscopic parameters of the copper(II) complexes formed with the synthesized peptide and those reported for the copper(II) binding sites of the whole protein. Herein, the synthesis of the human Dpl peptide fragment hDpl(122-139) (Ac-KPDNKLHQQVLWRLVQEL-NH(2)) and its copper(II) complex species are reported. This peptide encompasses the third alpha helix and part of the loop linking the second and the third helix of human doppel protein. The single-point-mutated peptide, hDpl(122-139)D124N, in which aspartate 124 replaces an asparagine residue, was also synthesized. This peptide was used to highlight the role of the carboxylate group on both the conformation preference of the Dpl fragment and its copper(II) coordination features. NMR spectroscopic measurements show that the hDpl(122-139) peptide fragment is in the prevailing alpha-helix conformation. It is localized within the 127-137 amino acid residue region that represents a reliable conformational mimic of the related protein domain. A comparison with the single-point-mutated hDpl(122-139)D124N reveals the significant role played by the aspartic residue in addressing the peptide conformation towards a helical structure. It is further confirmed by CD measurements. Potentiometric titrations were carried out in aqueous solutions to obtain the stability constant values of the species formed by copper(II) with the hDpl peptides. Spectroscopic studies (EPR, NMR, CD, UV/Vis) were performed to characterize the coordination environments of the different metal complexes. The EPR parameters of the copper(II) complexes with hDpl(122-139) match those of the previously reported copper(II) binding sites of the whole hDpl. Addition of the copper(II) ion to the peptide fragment does not alter the helical conformation of hDpl(122-139), as shown by CD spectra in the far-UV region. The aspartate-driven preorganized secondary structure is not significantly modified by the involvement of Asp124 in the copper(II) complex species that form in the physiological pH range. To elaborate on the potential role of copper(II) in the recently reported interaction between the PrP(C) and Dpl, the affinity of the copper(II) complexes towards the prion N terminus domain and the binding site of Dpl was reported.
authors.affiliation: Istituto di Biostrutture e Bioimmagini-CNR, c/o Dipartimento di Scienze Chimiche, Viale A. Doria 6, 95125 Catania, Italy.
authors.forename: Diego
authors.initials: D
authors.lastname: La Mendola
authors.forename: Antonio
authors.initials: A
authors.lastname: Magrì
authors.forename: Tiziana
authors.initials: T
authors.lastname: Campagna
authors.forename: Maria Anna
authors.initials: MA
authors.lastname: Campitiello
authors.forename: Luca
authors.initials: L
authors.lastname: Raiola
authors.forename: Carla
authors.initials: C
authors.lastname: Isernia
authors.forename: Orjan
authors.initials: O
authors.lastname: Hansson
authors.forename: Raffaele P
authors.initials: RP
authors.lastname: Bonomo
authors.forename: Enrico
authors.initials: E
authors.lastname: Rizzarelli
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D058851:GPI-Linked Proteins / Q000737:chemistry; D006801:Humans; D010455:Peptides / Q000737:chemistry; D011328:Prions / Q000737:chemistry*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D058851:GPI-Linked Proteins; C501125:PRND protein, human; D010455:Peptides; D011328:Prions; D003300:Copper