A significant proportion of F2-isoprostanes in human urine are excreted as glucuronide conjugates.
The measurement of urinary F(2)-isoprostanes is noninvasive and widely used to assess in vivo oxidative stress in humans. Most studies measure urinary F(2)-isoprostanes in the free form; however, many eicosanoids are excreted as urine glucuronide conjugates. Using gas chromatography-mass spectrometry, we examined the extent of glucuronide conjugation of F(2)-isoprostanes in urine collected from healthy men (n = 20) and women (n = 15). Incubation of urine with exogenous glucuronidase led to F(2)-isoprostane concentrations that were approximately 40% higher than untreated samples (P < 0.001). We conclude that a significant proportion of F(2)-isoprostanes in urine are conjugated as glucuronides.
Yan
Zhao
Z
Fourth Military Medical University, Xi'an 710032, China.
Mas
Emilie
E
Mori
Trevor A
TA
Croft
Kevin D
KD
Barden
Anne E
AE
D028441:F2-Isoprostanes / Q000378:metabolism* / Q000652:urine*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D020719:Glucuronides / Q000378:metabolism* / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged
D028441:F2-Isoprostanes; D020719:Glucuronides