Cardiomyocyte regeneration in pericicatricial zone of the myocardium after laser tunneling and implantation of bone marrow mononuclear cells.
Using canine model of chronic postinfarction myocardial ischemia we evaluated angiogenesis and cardiomyogenesis after combined revascularization by laser tunneling of the pericicatricial zone followed by implantation of bone marrow mononuclear cells into the channels. It was shown that the numerical density of arterioles and capillaries considerably increased 1 month after revascularization. We also observed a considerable increase in the density of cardiomyocyte nuclei in the test area, a 2-fold increase in the total area of nuclei, and an increase in the mean area of cardiomyocyte nuclei. The number of PNCA-positive cardiomyocytes significantly increased after combined revascularization. Our findings suggest that combined revascularization of the pericicatricial zone of the myocardium promoted angiogenesis and stimulates regeneration of cardiomyocytes.
Larionov
P M
PM
E. N. Meshalkin Research Institute of Circulatory Pathology, Federal Agency for High-Technological Medical Care, Novosibirsk, Russia. pathol@soramn.ru
Chernyavskii
A M
AM
Kuznetsova
I V
IV
Lushnikova
E L
EL
Bondar'
V Yu
VY
Uglova
E V
EV
Bocharova
A V
AV
Subbotina
O A
OA
Nepomnyashchikh
L M
LM
Karas'kov
A M
AM
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D004285:Dogs; D007834:Lasers; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000637:transplantation*; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation / Q000379:methods; D009206:Myocardium / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*; D018919:Neovascularization, Physiologic; D012038:Regeneration / Q000502:physiology*