title: Assessment of bile fluorescence patterns in a tropical fish, Nile tilapia (Oreochromis niloticus) exposed to naphthalene, phenanthrene, pyrene and chrysene using fixed wavelength fluorescence and synchronous fluorescence spectrometry.
abstract: Bile fluorescence patterns in Nile tilapia, a potential fish for biomonitoring tropical water pollution were assessed following exposure to selected polycyclic aromatic hydrocarbons (PAHs): naphthalene, phenanthrene, pyrene and chrysene. Non-normalized fixed wavelength fluorescence signals in the fish exposed to these PAHs reflected dose and/or time response relationships of their metabolism. Normalizing signals to biliverdin introduced deviations to these response patterns. The optimal wavelength pairs (excitation/emission) for synchronous fluorescence scanning measurements of bile metabolites of naphthalene, phenanthrene, pyrene and chrysene were identified as 284/326, 252/357, 340/382 and 273/382 respectively. This study supports the use of bile fluorescence in Nile tilapia by fixed wavelength fluorescence and synchronous fluorescence spectrometry with non-normalized data as a simple method for screening bioavailability of these PAHs.
authors.affiliation: Department of Zoology, Faculty of Science, University of Kelaniya, Kelaniya, Sri Lanka. asoka@kln.ac.lk
authors.forename: A
authors.initials: A
authors.lastname: Pathiratne
authors.forename: C K
authors.initials: CK
authors.lastname: Hemachandra
authors.forename: K A S
authors.initials: KA
authors.lastname: Pathiratne
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry / Q000378:metabolism*; D002911:Chrysenes / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D023681:Cichlids; D004784:Environmental Monitoring / Q000379:methods; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D009281:Naphthalenes / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D010616:Phenanthrenes / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D011721:Pyrenes / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*
chemical_list: D002911:Chrysenes; D005456:Fluorescent Dyes; D009281:Naphthalenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D014874:Water Pollutants, Chemical; C031180:chrysene; C031721:naphthalene; C031181:phenanthrene; C030984:pyrene