title: Interactions of the ribosome with mRNA and tRNA.
abstract: Recent collection of high-resolution crystal structures of the 70S ribosome with mRNA and tRNA substrates enhances our knowledge of protein synthesis principles. A novel network of interactions between the ribosome in the elongation state and mRNA downstream from the A codon suggests that mRNA is stabilized and aligned at the entrance to the decoding center. The X-ray studies clarify how natural modifications of tRNA are involved in the stabilization of the codon-anticodon interactions, prevention of frame-shifting and also expansion of the decoding capacity of tRNAs. In addition, the crystal structures provide the view that tRNA in the A and P sites communicate through a protein rich environment and suggest how these tRNAs are controlled through the intersubunit bridge formed by protein L31.
authors.affiliation: Département de Biologie et de Génomique Structurales, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch 67400, France.
authors.forename: Natalia
authors.initials: N
authors.lastname: Demeshkina
authors.forename: Lasse
authors.initials: L
authors.lastname: Jenner
authors.forename: Gulnara
authors.initials: G
authors.lastname: Yusupova
authors.forename: Marat
authors.initials: M
authors.lastname: Yusupov
mesh_terms: D001483:Base Sequence; D003062:Codon / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003062:Codon; D012333:RNA, Messenger; D012343:RNA, Transfer