Effect of microwave irradiation on cellular disintegration of Gram positive and negative cells.
This research investigated the effect of microwave irradiation (MWI) on cell disintegration in municipal secondary sludge (MSS). A representative MSS Gram-positive bacterium (Bacillus subtilis) and Gram-negative bacteria (Acinetobacter calcoaceticus and Pseudomonas aeruginosa) were pure cultured separately and treated using MWI. Compared to untreated controls, MWI significantly increased the soluble chemical oxygen demand (COD) (1.8-4.0-fold), soluble protein concentration (1.1-1.8-fold), and soluble carbohydrate concentration (3.2-14.1-fold), with greater increase in the Gram-negative bacteria. After MSS was MWI-treated with different irradiation times, from 0 to 9 min, soluble COD increased gradually from 0.14 to 2.38 g/L (i.e., 72-fold). Effective disintegration of Gram-negative cell walls and of MSS by MWI was confirmed by scanning electron microscopy. These findings suggest that MWI could be an effective pretreatment method for MSS that is dominated by Gram-negative microorganisms.
Zhou
Bi Wen
BW
School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, 790-784, South Korea.
Shin
Seung Gu
SG
Hwang
KwangHyun
K
Ahn
Johng-Hwa
JH
Hwang
Seokhwan
S
D006090:Gram-Negative Bacteria / Q000378:metabolism / Q000528:radiation effects*; D006094:Gram-Positive Bacteria / Q000378:metabolism / Q000528:radiation effects*; D050296:Microbial Viability / Q000528:radiation effects; D008872:Microwaves; D010100:Oxygen / Q000378:metabolism; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D018505:Waste Management / Q000379:methods*
D012722:Sewage; D010100:Oxygen