title: 2p24 Gain region harboring MYCN gene compared with MYCN amplified and nonamplified neuroblastoma: biological and clinical characteristics.
abstract: Although the role of MYCN amplification in neuroblastoma is well established, the biological and clinical characteristics of the 2p gain region harboring the MYCN gene remain unclear. The aim of this study was to compare the biological and clinical characteristics of these tumors with MYCN amplified and nonamplified neuroblastoma and to determine their impact on disease outcome. Samples from 177 patients were analyzed by fluorescence in situ hybridization, including MYCN, 1p, 17q, and 11q regions; 2p gain was identified in 25 patients, MYCN amplification in 31, and no amplification in 121 patients. Patients with 2p gain had a significantly worse 5-year event-free survival rate than patients with no MYCN amplified (P < 0.001), and an intermediate 5-year overall survival rate difference existed between the MYCN amplified tumors (P = 0.025) and nonamplified (P = 0.003) groups. All of the 2p gain samples were associated with segmental and/or numerical alterations in the other tested regions. The presence of segmental alterations with or without MYCN amplification was recently found to be the strongest predictor of relapse in a multivariate analysis. The results of the present study suggest that the determination of MYCN gene copy number relative to chromosome 2, when evaluating MYCN status at diagnosis, may help to reveal the underlying genetic pattern of these tumors and better understand their clinical behavior.
authors.affiliation: Ca-Cytogenetic Lab, Schneider Children's Medical Center of Israel, Kaplan St. 14, 49202 Petah Tikva, Israel. martaj@clalit.org.il
authors.forename: Marta
authors.initials: M
authors.lastname: Jeison
authors.forename: Shifra
authors.initials: S
authors.lastname: Ash
authors.forename: Gili
authors.initials: G
authors.lastname: Halevy-Berko
authors.forename: Jacques
authors.initials: J
authors.lastname: Mardoukh
authors.forename: Drorit
authors.initials: D
authors.lastname: Luria
authors.forename: Smadar
authors.initials: S
authors.lastname: Avigad
authors.forename: Galina
authors.initials: G
authors.lastname: Feinberg-Gorenshtein
authors.forename: Yacov
authors.initials: Y
authors.lastname: Goshen
authors.forename: Gabriel
authors.initials: G
authors.lastname: Hertzel
authors.forename: Joseph
authors.initials: J
authors.lastname: Kapelushnik
authors.forename: Ayelet
authors.initials: A
authors.lastname: Ben Barak
authors.forename: Dina
authors.initials: D
authors.lastname: Attias
authors.forename: Ran
authors.initials: R
authors.lastname: Steinberg
authors.forename: Jerry
authors.initials: J
authors.lastname: Stein
authors.forename: Batia
authors.initials: B
authors.lastname: Stark
authors.forename: Isaac
authors.initials: I
authors.lastname: Yaniv
mesh_terms: D002648:Child; D002889:Chromosomes, Human, Pair 2; D018572:Disease-Free Survival; D005784:Gene Amplification; D018628:Gene Dosage; D016259:Genes, myc; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D000071447:N-Myc Proto-Oncogene Protein; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D009687:Nuclear Proteins / Q000235:genetics*; D015513:Oncogene Proteins / Q000235:genetics*
chemical_list: C459801:MYCN protein, human; D000071447:N-Myc Proto-Oncogene Protein; D009687:Nuclear Proteins; D015513:Oncogene Proteins
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