title: Distribution and proliferation of bone marrow cells in the brain after pilocarpine-induced status epilepticus in mice.
abstract: The distribution of bone marrow cells in brain areas during the acute period after pilocarpine-induced status epiepticus (SE) was investigated here. To achieve this, we generated chimeric mice by engrafting bone marrow cells from enhanced green fluorescent protein (eGFP) transgenic mice. GFP(+) bone marrow-derived cells were found throughout the brain, predominantly in the hippocampus. As expected, these cells exhibited the characteristics of microglia. The pattern of distribution, proliferation, and differentiation of GFP(+)cells changes as a function of intensity and time following SE. This pattern is also a consequence of the inflammatory response, which is followed by the progressive neuronal damage that is characteristic of the pilocarpine model.
authors.affiliation: Laboratório de Engenharia Tecidual e Imunofarmacologia, CPqGM/FIOCRUZ, Bahia, Brazil.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Longo
authors.forename: Simone
authors.initials: S
authors.lastname: Romariz
authors.forename: Miriam Marcela
authors.initials: MM
authors.lastname: Blanco
authors.forename: Juliana Fraga
authors.initials: JF
authors.lastname: Vasconcelos
authors.forename: Luciana
authors.initials: L
authors.lastname: Bahia
authors.forename: Milena Botelho Pereira
authors.initials: MB
authors.lastname: Soares
authors.forename: Luiz E
authors.initials: LE
authors.lastname: Mello
authors.forename: Ricardo
authors.initials: R
authors.lastname: Ribeiro-dos-Santos
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D001921:Brain / Q000473:pathology / Q000601:surgery*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D049109:Cell Proliferation; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010862:Pilocarpine / Q000009:adverse effects; D013226:Status Epilepticus / Q000139:chemically induced / Q000473:pathology / Q000601:surgery*; D013997:Time Factors
chemical_list: D009419:Nerve Tissue Proteins; C485184:enhanced green fluorescent protein; D010862:Pilocarpine; D049452:Green Fluorescent Proteins; D001973:Bromodeoxyuridine