title: On-chip differential interference contrast microscopy using lensless digital holography.
abstract: We introduce the use of a birefringent crystal with lensless digital holography to create an on-chip differential interference contrast (DIC) microscope. Using an incoherent source with a large aperture, in-line holograms of micro-objects are created, which interact with a uniaxial crystal and an absorbing polarizer, encoding differential interference contrast information of the objects on the chip. Despite the fact that a unit fringe magnification and an incoherent source with a large aperture have been used, holographic digital processing of such holograms rapidly recovers the differential phase contrast image of the specimen over a large field-of-view of approximately 24 mm(2).
authors.affiliation: Electrical Engineering Department, University of California, Los Angeles, CA, USA.
authors.forename: Chulwoo
authors.initials: C
authors.lastname: Oh
authors.forename: Serhan O
authors.initials: SO
authors.lastname: Isikman
authors.forename: Bahar
authors.initials: B
authors.lastname: Khademhosseinieh
authors.forename: Aydogan
authors.initials: A
authors.lastname: Ozcan
references.citation: J Opt Soc Am. 1967 Apr;57(4):546-7
references.pmid: 6027838
references.citation: Opt Express. 2006 Sep 4;14(18):8263-8
references.pmid: 19529201
references.citation: Opt Express. 2005 Oct 31;13(22):8693-8
references.pmid: 19498901
references.citation: Opt Lett. 2006 May 15;31(10):1405-7
references.pmid: 16642120
references.citation: Appl Opt. 2007 Apr 1;46(10):1836-42
references.pmid: 17356628
references.citation: J Microsc. 2002 Jun;206(Pt 3):194-203
references.pmid: 12067363
references.citation: J Opt Soc Am A Opt Image Sci Vis. 2006 Apr;23(4):926-32
references.pmid: 16604777
references.citation: Methods Cell Biol. 2008;90:87-115
references.pmid: 19195547
references.citation: Phys Rev Lett. 2000 Oct 16;85(16):3385-7
references.pmid: 11030902
references.citation: Opt Lett. 2004 May 15;29(10):1132-4
references.pmid: 15182009
references.citation: Opt Lett. 2007 Dec 15;32(24):3492-4
references.pmid: 18087519
references.citation: Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11301-5
references.pmid: 11572982
references.citation: Opt Lett. 1985 Jan 1;10(1):4-6
references.pmid: 19724327
references.citation: Opt Lett. 2006 May 1;31(9):1211-3
references.pmid: 16642062
references.citation: Opt Express. 2006 May 1;14(9):3792-805
references.pmid: 19516527
references.citation: Appl Opt. 1992 Aug 20;31(24):4973-8
references.pmid: 20733659
references.citation: Appl Opt. 2002 Aug 1;41(22):4489-96
references.pmid: 12153076
references.citation: Opt Lett. 1978 Jul 1;3(1):27-9
references.pmid: 19684685