Salicylate-induced translocation of prestin having mutation in the GTSRH sequence to the plasma membrane.
Prestin is a key molecule for mammalian hearing. The present study investigated changes in characteristics of prestin by culturing prestin-transfected cells with salicylate, an antagonist of prestin. As a result, the plasma membrane localization of prestin bearing a mutation in the GTSRH sequence, which normally accumulates in the cytoplasm, was recovered. Moreover, the nonlinear capacitance of the majority of the mutants, which is a signature of prestin activity, was also recovered. Thus, the present study discovered a new effect of salicylate on prestin, namely, the promotion of the plasma membrane expression of prestin mutants in an active state.
Kumano
Shun
S
Department of Bioengineering and Robotics, Tohoku University, Sendai, Japan.
Iida
Koji
K
Ishihara
Kenji
K
Murakoshi
Michio
M
Tsumoto
Kouhei
K
Ikeda
Katsuhisa
K
Kumagai
Izumi
I
Kobayashi
Toshimitsu
T
Wada
Hiroshi
H
D000818:Animals; D001483:Base Sequence; D001669:Biochemical Phenomena; D002462:Cell Membrane / Q000235:genetics* / Q000378:metabolism*; D002465:Cell Movement / Q000235:genetics; D025802:Electric Capacitance; D006309:Hearing / Q000235:genetics; D017136:Ion Transport / Q000235:genetics; D008322:Mammals / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D021381:Protein Transport / Q000235:genetics; D020156:Salicylic Acid / Q000378:metabolism*
D020156:Salicylic Acid