An array platform for identification of stress-responsive microRNAs in plants.
MicroRNAs (miRNAs) are approximately 22-nucleotide (nt)-long non-coding RNAs that play a key role in plant development and abiotic stresses. We have developed a simple but effective array platform for profiling plant miRNAs from various plant species. The array is composed of 188 non-redundant miRNA probes that can detect both conserved and species-specific miRNAs from most plant species, including Arabidopsis, rice, and poplar. In this chapter, we describe a protocol for developing the miRNA array platform, which can be used to identify stress-responsive miRNAs in diverse plant species. Profiling of miRNAs in tobacco seedlings exposed to different abiotic stress conditions has revealed that a number of miRNAs, miR398, miR399, miR408, miR156, miR164, and miR168, were responsive to stresses. This comprehensive and easy-to-follow protocol will be useful for studying roles of miRNAs in plant stress response.
Jia
Xiaoyun
X
Department of Plant and Soil Sciences and KTRDC, University of Kentucky, Lexington, KY, USA.
Mendu
Venugopal
V
Tang
Guiliang
G
D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015347:RNA Probes / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D013312:Stress, Physiological / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*
D035683:MicroRNAs; D015347:RNA Probes; D018749:RNA, Plant