Direct evaluation of cellular internalization rates using chromogenic disulfides.
A practical assay, which exploits the maintenance of the cytoplasmic redox balance in live cells, has been developed to report in real time on relative cellular internalization rates of molecules derivatized as chromogenic disulfides.
Bucks
Megan E
ME
Department of Chemistry, Purdue University, West Lafayette, IN 47909, USA.
Savinov
Sergey N
SN
D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D002863:Chromogenic Compounds / Q000737:chemistry / Q000378:metabolism*; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D008954:Models, Biological; D015394:Molecular Structure
D002863:Chromogenic Compounds; D004220:Disulfides