A novel potassium channel in photosynthetic cyanobacteria.
Elucidation of the structure-function relationship of a small number of prokaryotic ion channels characterized so far greatly contributed to our knowledge on basic mechanisms of ion conduction. We identified a new potassium channel (SynK) in the genome of the cyanobacterium Synechocystis sp. PCC6803, a photosynthetic model organism. SynK, when expressed in a K(+)-uptake-system deficient E. coli strain, was able to recover growth of these organisms. The protein functions as a potassium selective ion channel when expressed in Chinese hamster ovary cells. The location of SynK in cyanobacteria in both thylakoid and plasmamembranes was revealed by immunogold electron microscopy and Western blotting of isolated membrane fractions. SynK seems to be conserved during evolution, giving rise to a TPK (two-pore K(+) channel) family member which is shown here to be located in the thylakoid membrane of Arabidopsis. Our work characterizes a novel cyanobacterial potassium channel and indicates the molecular nature of the first higher plant thylakoid cation channel, opening the way to functional studies.
Zanetti
Manuela
M
Department of Biology, University of Padova, Padova, Italy.
Teardo
Enrico
E
La Rocca
Nicoletta
N
Zulkifli
Lalu
L
Checchetto
Vanessa
V
Shijuku
Toshiaki
T
Sato
Yuki
Y
Giacometti
Giorgio Mario
GM
Uozumi
Noboyuki
N
Bergantino
Elisabetta
E
Szabò
Ildikò
I
D017360:Arabidopsis / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D010788:Photosynthesis; D015221:Potassium Channels / Q000032:analysis* / Q000235:genetics; D046939:Synechocystis / Q000737:chemistry* / Q000235:genetics; D020524:Thylakoids / Q000737:chemistry
D001426:Bacterial Proteins; D015221:Potassium Channels
J Biol Chem. 2007 Aug 17;282(33):24294-301
17588940
J Biol Chem. 2004 Dec 24;279(52):54952-62
15459199
J Biol Chem. 2006 Jul 14;281(28):19645-54
16679322
Nature. 2003 Mar 13;422(6928):180-5
12629550
Biophys J. 1994 Apr;66(4):1061-7
8038378
FEBS Lett. 2005 Oct 24;579(25):5814-20
16223486
Eur J Biochem. 1998 May 01;253(3):606-13
9654056
FEMS Microbiol Rev. 2005 Nov;29(5):961-85
16026885
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):15265-70
14657329
FEBS Lett. 2003 Sep 18;552(1):12-6
12972145
J Biol Chem. 2009 Jun 12;284(24):16513-16521
19372598
Nature. 2002 Oct 17;419(6908):715-8
12384697
Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13443-8
11687660
Plant Physiol. 1995 Aug;108(4):1725-1734
12228576
DNA Res. 1996 Jun 30;3(3):109-36
8905231
J Exp Biol. 1998 Oct;201(Pt 20):2791-9
9866872
J Biol Chem. 2000 Dec 15;275(50):39420-6
10970888
EMBO J. 1995 Nov 1;14(21):5170-8
7489706
Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12246-51
12218172
Nature. 2007 Jan 4;445(7123):116-9
17167423
Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15831-6
9861056
J Gen Physiol. 2006 Sep;128(3):283-92
16908725
EMBO Rep. 2005 May;6(5):420-5
15864293
EMBO J. 2007 Jan 10;26(1):232-41
17159901
Plant Physiol. 2001 Aug;126(4):1646-67
11500563
J Cell Sci. 2004 May 15;117(Pt 12):2449-60
15159450
Biochim Biophys Acta. 2007 Jun;1767(6):703-11
17250801
Prog Nucleic Acid Res Mol Biol. 2003;75:293-320
14604015
Biochemistry. 1997 Aug 19;36(33):10343-52
9254634
Plant Physiol. 1989 Sep;91(1):249-52
16667005
Biosci Biotechnol Biochem. 2006 Jan;70(1):273-5
16428848
Plant J. 2004 Dec;40(6):845-59
15584951
Annu Rev Neurosci. 1997;20:91-123
9056709
Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):14861-6
18818304
Arch Microbiol. 1997 Feb-Mar;167(2-3):126-36
9133319
Mol Microbiol. 2008 Oct;70(1):140-50
18699865
Am J Physiol Cell Physiol. 2001 Sep;281(3):C733-9
11502550
Plant J. 2006 Oct;48(2):296-306
16984403
Nature. 1999 Dec 16;402(6763):817-21
10617203
FEBS Lett. 1999 May 7;450(3):217-20
10359077
Plant Cell Physiol. 2009 Apr;50(4):773-88
19224954
FEBS Lett. 2003 Jul 31;548(1-3):53-8
12885407
J Membr Biol. 1996 Aug;152(3):223-33
8672080