title: A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus.
abstract: Mycoviruses are viruses that infect fungi and have the potential to control fungal diseases of crops when associated with hypovirulence. Typically, mycoviruses have double-stranded (ds) or single-stranded (ss) RNA genomes. No mycoviruses with DNA genomes have previously been reported. Here, we describe a hypovirulence-associated circular ssDNA mycovirus from the plant pathogenic fungus Sclerotinia sclerotiorum. The genome of this ssDNA virus, named Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), is 2166 nt, coding for a replication initiation protein (Rep) and a coat protein (CP). Although phylogenetic analysis of Rep showed that SsHADV-1 is related to geminiviruses, it is notably distinct from geminiviruses both in genome organization and particle morphology. Polyethylene glycol-mediated transfection of fungal protoplasts was successful with either purified SsHADV-1 particles or viral DNA isolated directly from infected mycelium. The discovery of an ssDNA mycovirus enhances the potential of exploring fungal viruses as valuable tools for molecular manipulation of fungi and for plant disease control and expands our knowledge of global virus ecology and evolution.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yu
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Yanping
authors.initials: Y
authors.lastname: Fu
authors.forename: Daohong
authors.initials: D
authors.lastname: Jiang
authors.forename: Said A
authors.initials: SA
authors.lastname: Ghabrial
authors.forename: Guoqing
authors.initials: G
authors.lastname: Li
authors.forename: Youliang
authors.initials: Y
authors.lastname: Peng
authors.forename: Jiatao
authors.initials: J
authors.lastname: Xie
authors.forename: Jiasen
authors.initials: J
authors.lastname: Cheng
authors.forename: Junbin
authors.initials: J
authors.lastname: Huang
authors.forename: Xianhong
authors.initials: X
authors.lastname: Yi
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics / Q000472:pathogenicity* / Q000821:virology*; D001483:Base Sequence; D017124:Conserved Sequence; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D017791:Geminiviridae / Q000235:genetics / Q000472:pathogenicity* / Q000648:ultrastructure; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D016415:Sequence Alignment; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics; D014771:Virion / Q000648:ultrastructure; D014774:Virulence
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases
references.citation: J Virol. 2004 Jan;78(2):892-8
references.pmid: 14694120
references.citation: BMC Evol Biol. 2009 May 21;9:112
references.pmid: 19460138
references.citation: Virology. 1983 Oct 15;130(1):21-8
references.pmid: 18639136
references.citation: Nucleic Acids Res. 1995 Mar 25;23(6):910-6
references.pmid: 7731803
references.citation: J Gen Virol. 2006 Jan;87(Pt 1):241-249
references.pmid: 16361437
references.citation: Trends Plant Sci. 2006 May;11(5):209-12
references.pmid: 16616578
references.citation: Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3879-83
references.pmid: 7732000
references.citation: Trends Plant Sci. 2003 Mar;8(3):128-34
references.pmid: 12663223
references.citation: Annu Rev Phytopathol. 2005;43:361-94
references.pmid: 16078889
references.citation: Proc Biol Sci. 2002 Nov 7;269(1506):2269-76
references.pmid: 12455515
references.citation: Nat Rev Microbiol. 2005 Aug;3(8):632-42
references.pmid: 16064055
references.citation: Virology. 1992 Apr;187(2):633-42
references.pmid: 1546458
references.citation: Methods Mol Biol. 1998;81:41-52
references.pmid: 9760492
references.citation: J Virol. 2004 Mar;78(5):2405-13
references.pmid: 14963136
references.citation: FEBS Lett. 2009 Jun 18;583(12):1825-32
references.pmid: 19497325
references.citation: Plant Biotechnol J. 2009 Apr;7(3):254-65
references.pmid: 19175519
references.citation: Microbiol Mol Biol Rev. 2000 Sep;64(3):489-502
references.pmid: 10974123
references.citation: Science. 1982 Jan 29;215(4532):466-71
references.pmid: 17771259
references.citation: Curr Opin Plant Biol. 2006 Apr;9(2):209-15
references.pmid: 16480918
references.citation: Science. 2004 Apr 2;304(5667):66-74
references.pmid: 15001713
references.citation: Virol J. 2009 Jul 07;6:96
references.pmid: 19583873
references.citation: Arch Virol. 2008;153(8):1441-51
references.pmid: 18566736
references.citation: Science. 2007 Mar 30;315(5820):1786-7
references.pmid: 17395806
references.citation: Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):7088-93
references.pmid: 9192696
references.citation: Nucleic Acids Res. 1985 Apr 11;13(7):2189-206
references.pmid: 4000956
references.citation: FEMS Microbiol Lett. 2005 Feb 15;243(2):323-9
references.pmid: 15686831
references.citation: Plant Methods. 2009 Jul 02;5:9
references.pmid: 19573239
references.citation: Genetics. 2001 Sep;159(1):107-18
references.pmid: 11560890
references.citation: Mol Plant Microbe Interact. 2003 Sep;16(9):785-95
references.pmid: 12971602
references.citation: J Gen Virol. 1997 Aug;78 ( Pt 8):2113-7
references.pmid: 9267015
references.citation: Science. 1992 Aug 7;257(5071):800-3
references.pmid: 1496400
references.citation: Methods Enzymol. 1996;266:131-41
references.pmid: 8743682
references.citation: J Virol. 2009 Feb;83(4):1981-91
references.pmid: 19073734