title: Misleading results of the MboII-based identification of type 2a canine parvovirus strains from Hungary reacting as type 2c strains.
abstract: Type 2 canine parvovirus (CPV2) infection is one of the most frequent causes of death in the young, susceptible canine populations worldwide. Since its emergence in the 1970s, several variants have been described. In the present study the authors describe the genetic analysis of 24 Hungarian CPV2 strains collected from 2004 to 2008. Surprisingly, the genetic and phylogenetic investigations of all these strains revealed that all of them were type 2a CPVs. On the other hand, the genetic analysis provided substantial evidence to demonstrate that due to a seemingly constant point mutation present in most of the Hungarian CPV2a strains, a previously described MboII-based rapid identification of CPV2c strains unfortunately cannot be reliably used any more.
authors.affiliation: Department of Pathology and Forensic Veterinary Medicine, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest, 1078, Hungary. Demeter.Zoltan@aotk.szie.hu
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Demeter
authors.forename: Elena Alina
authors.initials: EA
authors.lastname: Palade
authors.forename: Tibor
authors.initials: T
authors.lastname: Soós
authors.forename: Attila
authors.initials: A
authors.lastname: Farsang
authors.forename: Csaba
authors.initials: C
authors.lastname: Jakab
authors.forename: Miklós
authors.initials: M
authors.lastname: Rusvai
mesh_terms: D000818:Animals; D004285:Dogs; D005260:Female; D016678:Genome; D005838:Genotype; D006814:Hungary; D008297:Male; D017993:Parvovirus, Canine / Q000235:genetics* / Q000302:isolation & purification; D014773:Virology / Q000379:methods
references.citation: Science. 1985 Nov 29;230(4729):1046-8
references.pmid: 4059921
references.citation: J Gen Virol. 2001 Dec;82(Pt 12):3021-3025
references.pmid: 11714979
references.citation: Vet Rec. 1996 May 18;138(20):495-6
references.pmid: 8736503
references.citation: J Vet Med B Infect Dis Vet Public Health. 2006 Dec;53(10):468-72
references.pmid: 17123424
references.citation: J Virol Methods. 2006 Apr;133(1):92-9
references.pmid: 16313976
references.citation: Arch Virol. 1979;61(1-2):1-11
references.pmid: 316320
references.citation: J Virol Methods. 2009 Aug;159(2):141-5
references.pmid: 19490967
references.citation: New Microbiol. 2008 Jan;31(1):125-30
references.pmid: 18437851
references.citation: Vet Rec. 2009 Feb 14;164(7):213-6
references.pmid: 19218594
references.citation: Schweiz Arch Tierheilkd. 2000 Mar;142(3):115-9
references.pmid: 10748710
references.citation: New Microbiol. 2000 Jan;23(1):93-6
references.pmid: 10946411
references.citation: J Vet Med B Infect Dis Vet Public Health. 2005 Sep-Oct;52(7-8):316-9
references.pmid: 16316391
references.citation: Aust Vet J. 1978 Dec;54(12):593
references.pmid: 753224
references.citation: Emerg Infect Dis. 2007 Aug;13(8):1222-4
references.pmid: 17953097
references.citation: J Gen Virol. 2008 Sep;89(Pt 9):2280-2289
references.pmid: 18753238
references.citation: J Vet Diagn Invest. 2005 Mar;17(2):133-8
references.pmid: 15825493
references.citation: Onderstepoort J Vet Res. 1998 Dec;65(4):239-42
references.pmid: 10192835
references.citation: Vet Microbiol. 2008 Sep 18;131(1-2):47-56
references.pmid: 18400421
references.citation: J Virol. 1991 Dec;65(12):6544-52
references.pmid: 1942246
references.citation: J Vet Diagn Invest. 2007 Sep;19(5):535-9
references.pmid: 17823398
references.citation: Virus Genes. 2009 Apr;38(2):249-58
references.pmid: 19112611
references.citation: J Clin Microbiol. 2004 Mar;42(3):1333-6
references.pmid: 15004112
references.citation: Vet Rec. 2009 May 9;164(19):593-5
references.pmid: 19429938
references.citation: Acta Vet Hung. 1999;47(3):379-94
references.pmid: 10497831
references.citation: Arch Virol. 2004 Nov;149(11):2261-9
references.pmid: 15503211
references.citation: Vet Rec. 1995 Feb 18;136(7):174-5
references.pmid: 7762129
references.citation: Res Vet Sci. 2010 Feb;88(1):169-71
references.pmid: 19552931
references.citation: Vet Microbiol. 2007 Sep 20;124(1-2):147-52
references.pmid: 17531408
references.citation: Vet Microbiol. 2006 Oct 5;117(1):9-13
references.pmid: 16765539
references.citation: Vet Microbiol. 1993 Dec;38(1-2):1-10
references.pmid: 8128593
references.citation: Vet Rec. 1986 Mar 22;118(12):332-3
references.pmid: 3705371
references.citation: J Vet Med B Infect Dis Vet Public Health. 2005 Sep-Oct;52(7-8):312-5
references.pmid: 16316390
references.citation: Vet Microbiol. 2000 Jul 31;75(2):127-33
references.pmid: 10889403
references.citation: Vet Rec. 1996 Apr 13;138(15):365-6
references.pmid: 8737265
references.citation: J Vet Med Sci. 2008 Dec;70(12):1305-14
references.pmid: 19122396
references.citation: Emerg Infect Dis. 2002 Apr;8(4):341-6
references.pmid: 11971764
references.citation: Proc Natl Acad Sci U S A. 2005 Jan 11;102(2):379-84
references.pmid: 15626758
references.citation: Vaccine. 2007 Jan 26;25(7):1161-6
references.pmid: 17092616
references.citation: Vet Rec. 1979 Aug 25;105(8):156-9
references.pmid: 516347