[Influence of N-acylethanolamines on embryonic cell culture survival under the effect of irradiation].
The effects of the N-stearoylethanolamine (NSE), N-oleoylethanolamine (OEA) and N-acylethanolamine (NAE) mixture on the cell survival, apoptosis and activity of mitochondrial succinate dehydrogenase (SDG) and glycerophosphate dehydrogenase (GFDG) in embryonic cell culture under normal conditions and irradiation were compared in the work. It was shown, that all investigated NAE were able to modulate the proliferative activity of intact cells as well as irradiation-exposed cells in concentration-depended manner. The most pronounced effect was observed under the NAE mixture action. It was established, that NAE prevented the damage effects of the irradiation and diminished the activation of apoptotic cell death. It was found that NSE and OEA decreased the activity of the SDG (42.3 and 44.14%, accordingly) and the GFDG activity (14.67 and 17.33%, accordingly) in embryonic cell culture, while addition of the NAE mixture decreased SDG activity by 20%, at the same time GFDG activity increased by 20%. Our findings suggested that antiproliferative effects of NAE depended on their influence on mitochondrial functioning.
Asmolkova
V S
VS
Lavrenchuk
H I
HI
Khmel'
T O
TO
Hula
N M
NM
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D004983:Ethanolamines / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D008928:Mitochondria; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D032342:Muscle Cells / Q000187:drug effects / Q000528:radiation effects; D009132:Muscles / Q000166:cytology / Q000196:embryology; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D051381:Rats; D013385:Succinate Dehydrogenase / Q000378:metabolism
D000975:Antioxidants; D004983:Ethanolamines; C022203:N-acylethanolamines; D005993:Glycerolphosphate Dehydrogenase; D013385:Succinate Dehydrogenase