Metabolic versatility of halotolerant and alkaliphilic strains of Halomonas isolated from alkaline black liquor.
Wheat straw black liquor is a notorious pulp mill wastewater with very high pH and pollution load. Two halotolerant and alkaliphilic bacteria, designated as Halomonas sp. 19-A and Y2, were isolated from wheat straw black liquor and shown to be able to use guaiacol, vanillin, dibenzo-p-dioxin, biphenyl and fluorene, as sole carbon and carbazole as sole carbon and nitrogen source at pH 9.5 and in the presence of 10% NaCl. The two strains produced carboxymethylcellulase (CMCase), xylanase, lipase, amylase, and pullulanase. High activities of CMCase, xylanase, and amylase were observed at pH 5.0-11.0 and NaCl concentrations of 0-15%. The metabolic versatility of these Halomonas strains even under extreme pH and salinity conditions makes them promising agents for bioremediation and industrial processes.
Yang
Chunyu
C
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Wang
Zhe
Z
Li
Yang
Y
Niu
Yu
Y
Du
Miaofen
M
He
Xiaofei
X
Ma
Cuiqing
C
Tang
Hongzhi
H
Xu
Ping
P
D000222:Adaptation, Physiological; D000468:Alkalies; D004798:Enzymes / Q000378:metabolism; D020632:Halomonas / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D000468:Alkalies; D004798:Enzymes; D012336:RNA, Ribosomal, 16S