Down-regulation of Dicer in hepatocellular carcinoma.
Dicer, the key enzyme in the RNAi pathway, is misregulated in tumor tissues. The altered expression of Dicer is associated with clinical characteristics in patients with cancer. Liver carcinoma and adjacent non-neoplastic tissues were obtained from 36 patients with hepatocellular carcinoma (HCC) undergoing surgery. Expressions of Dicer mRNA were evaluated using the Real-time reverse transcription-PCR in 36 liver carcinoma tissues and 36 adjacent histologically non-cancerous liver tissues. Dicer mRNA levels were evaluated in relation to age, sex, tumor number, tumor size, tumor stage, and distant metastasis. Dicer mRNA level was significantly lower in malignant tissues than in the corresponding non-neoplastic tissues in 34 of the 36 patients with HCC (94.4%). The Dicer expression level was not associated with clinical characteristics, including age, sex, tumor number, tumor size, tumor stage, or distant metastasis in HCC cases. These results demonstrate that Dicer is significantly down-regulated in HCC, suggesting that reduced expression of Dicer may play an important role during the process of hepatocarcinogenesis.
Wu
Jin-Feng
JF
Key Laboratory of Molecular Biology for Infectious Diseases of the State Ministry of Education, Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical University, 74# Linjiang Road, 400010, Chongqing, People's Republic of China.
Shen
Wei
W
Liu
Nian-Zhou
NZ
Zeng
Gui-Li
GL
Yang
Mei
M
Zuo
Guo-Qing
GQ
Gan
Xiu-Ni
XN
Ren
Hong
H
Tang
Kai-Fu
KF
D000328:Adult; D000368:Aged; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043244:Ribonuclease III / Q000096:biosynthesis*
D012333:RNA, Messenger; D043244:Ribonuclease III
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2999-3004
14973191
Cancer Sci. 2005 Feb;96(2):111-5
15723655
J Gastroenterol Hepatol. 2006 Jan;21(1 Pt 1):15-21
16706806
Oncogene. 2008 Sep 25;27(43):5651-61
18521080
Gut. 2001 Jan;48(1):103-9
11115830
Gastroenterology. 2007 Aug;133(2):647-58
17681183
Int J Cancer. 2009 Jun 15;124(12):2855-63
19347935
Am J Pathol. 2006 Nov;169(5):1812-20
17071602
Cancer Res. 2008 Jul 1;68(13):5049-58
18593903
Nat Rev Cancer. 2006 Nov;6(11):857-66
17060945
Oncogene. 2002 Apr 11;21(16):2593-604
11971194
Leuk Res. 2009 Aug;33(8):e127
19278725
Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9136-41
16754881
J Exp Med. 2006 Oct 30;203(11):2519-27
17060477
J Clin Virol. 2005 Dec;34 Suppl 1:S1-3
16461208
N Engl J Med. 2008 Dec 18;359(25):2641-50
19092150
Mod Pathol. 2008 Jun;21(6):676-84
18327211
Ann Surg. 1999 Jun;229(6):790-9; discussion 799-800
10363892
Nat Rev Cancer. 2006 Apr;6(4):259-69
16557279
Curr Opin Gen Surg. 1993;:257-64
7583984
Oncogene. 2006 Apr 20;25(17):2537-45
16331254
Hepatology. 2008 Apr;47(4):1223-32
18307259
Cancer Res. 2007 Mar 1;67(5):2345-50
17332367
Cancer Res. 2007 Jul 1;67(13):6092-9
17616664
Hepatology. 2008 Jun;47(6):1955-63
18433021
Gastroenterology. 2008 Jul;135(1):257-69
18555017
Gastroenterology. 2009 Jun;136(7):2304-2315.e1-4
19272382
Hepatology. 2009 Apr;49(4):1194-202
19133651
Int J Cancer. 2008 Oct 1;123(7):1616-22
18649363