title: Progesterone supplementation during multiple ovulation treatment in buffalo species (Bubalus bubalis).
abstract: The aim of this study was to evaluate the effect of exogenous progesterone supplementation on superovulatory response in buffaloes that has undergone a multiple ovulation program. Fourteen Mediterranean buffaloes were divided into two groups and received a 4-day decreasing dosage of an equal mixture of 500 IU of FSH and LH starting on day 8 of the cycle. In group A (n = 7) a progesterone-releasing intravaginal device was removed on day 8, whereas in group B (n = 7) it was left till day 10, when PGF2alpha was administered. Eighty hours later, buffaloes were artificially inseminated and after 6 days they undergone uterine flushing. A higher (P < 0.05) number of corpora lutea (8.3 vs. 5.7) and embryo/flushing/buffalo (2.3 vs. 1.3) were recorded in group B vs. group A if responsive buffaloes are considered (n = 12) and the number of corpora lutea was highly correlated with the number of embryos (r = 0.65; P < 0.05). In conclusion, progesterone supplementation during the first 2 days of the superovulation treatment seems to enhance the recovery rate in buffalo species. A high ovulation rate, associated with a high number of corpora lutea, can represent a parameter for estimating embryo recovery.
authors.affiliation: DISCIZIA, Faculty of Veterinary Medicine, Federico II University, Naples, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Neglia
authors.forename: Bianca
authors.initials: B
authors.lastname: Gasparrini
authors.forename: Domenico
authors.initials: D
authors.lastname: Vecchio
authors.forename: Marcello
authors.initials: M
authors.lastname: Rubessa
authors.forename: Rossella
authors.initials: R
authors.lastname: Di Palo
authors.forename: Luigi
authors.initials: L
authors.lastname: Zicarelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Campanile
mesh_terms: D000282:Administration, Intravaginal; D000818:Animals; D002020:Buffaloes / Q000502:physiology*; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D007558:Italy; D010062:Ovulation Induction / Q000379:methods* / Q000662:veterinary*; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D011374:Progesterone
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