title: Amnion-derived cellular cytokine solution (ACCS) promotes migration of keratinocytes and fibroblasts.
abstract: Amnion-derived Multipotent Progenitor cells appear to be useful as adjuvants in wound healing. Amnion-derived multipotent progenitor cells secrete a unique combination of cytokines and growth factors, known as amnion-derived cellular cytokine solution (ACCS). In the skin, a cytokine communication network between mesenchymal and epithelial cells tightly controls keratinocyte and fibroblast migration, proliferation and differentiation-key determinants of wound healing. To evaluate the influence of ACCS on the migratory behavior of keratinocytes and fibroblasts, cell migration was assayed quantitatively using a Boyden chamber. Chemotactic migration activity of fibroblasts or keratinocytes through the membrane determined the influence of ACCS. In the presence of ACCS, fibroblasts and keratinocytes demonstrated a statistically significant (P < 0.05) increase in migration when compared with controls. These cell types, critical to normal wound healing, may be influenced to accelerate migration in wounds, thus accelerating wound repair/healing.
authors.affiliation: Institute for Tissue Regeneration, Repair, and Rehabilitation, Bay Pines VA Healthcare System, Bay Pines, FL, USA.
authors.forename: M Georgina
authors.initials: MG
authors.lastname: Uberti
authors.forename: Yvonne N
authors.initials: YN
authors.lastname: Pierpont
authors.forename: Francis
authors.initials: F
authors.lastname: Ko
authors.forename: Terry E
authors.initials: TE
authors.lastname: Wright
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Smith
authors.forename: C Wayne
authors.initials: CW
authors.lastname: Cruse
authors.forename: Martin C
authors.initials: MC
authors.lastname: Robson
authors.forename: Wyatt G
authors.initials: WG
authors.lastname: Payne
mesh_terms: D000650:Amnion / Q000166:cytology*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D011076:Polycarboxylate Cement; D012996:Solutions; D054457:Tissue Scaffolds
chemical_list: D016207:Cytokines; D011076:Polycarboxylate Cement; D012996:Solutions; C005506:polycarbonate