title: Relation among neuronal death, cell proliferation and neuronal differentiation in the gerbil main olfactory bulb after transient cerebral ischemia.
abstract: Neurogenesis occurs during the embryonic stage and throughout life. Brain injuries such as ischemic insults enhance cell proliferation in some areas of the brain. We examined proliferation of newly generated cells in each layer of the gerbil main olfactory bulb (MOB) after 5 min of transient cerebral ischemia using bromodeoxyuridine (BrdU) immunohistochemistry. Ischemia-related neuronal death in the MOB was not detected using Fluoro-Jade B histofluorescence and TUNEL staining. Many BrdU-positive ((+)) cells were found in the rostral migratory stream in control and ischemic MOBs. Significant increase of BrdU(+) cells was observed in the granule cell layer (GCL) and glomerular layer (GL) from 15 days post-ischemia, and BrdU(+) cells were very much higher than those of the control group 30 days post-ischemia. At this time point after ischemia/reperfusion, a few BrdU(+) cells in the GL and GCL were co-localized with calretinin(+) cells, and many BrdU(+) cells expressed doublecortin, a marker of immature neurons. These results indicate that cell proliferation is increased in the GCL and GL without apparent neuronal loss from 15 days after transient cerebral ischemia in gerbils.
authors.affiliation: Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea.
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Ok Kyu
authors.initials: OK
authors.lastname: Park
authors.forename: Choong Hyun
authors.initials: CH
authors.lastname: Lee
authors.forename: Sung Koo
authors.initials: SK
authors.lastname: Kim
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Yun Lyul
authors.initials: YL
authors.lastname: Lee
authors.forename: Hyung-Cheul
authors.initials: HC
authors.lastname: Shin
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001973:Bromodeoxyuridine / Q000378:metabolism; D064032:Calbindin 2; D016923:Cell Death; D002454:Cell Differentiation; D049109:Cell Proliferation; D000089622:Doublecortin Domain Proteins; D005455:Fluorescent Antibody Technique; D005849:Gerbillinae / Q000378:metabolism*; D020287:In Situ Nick-End Labeling; D002546:Ischemic Attack, Transient / Q000378:metabolism / Q000473:pathology*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D009479:Neuropeptides / Q000378:metabolism; D009830:Olfactory Bulb / Q000378:metabolism / Q000473:pathology*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism
chemical_list: D064032:Calbindin 2; D000089622:Doublecortin Domain Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D064030:S100 Calcium Binding Protein G; D001973:Bromodeoxyuridine
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