title: Oligonucleotide-based tools for studying zebrafish development.
abstract: Synthetic and nonnatural oligonucleotides have been used extensively to interrogate gene function in zebrafish. In this review, we survey the capabilities and limitations of various oligonucleotide-based technologies for perturbing RNA function and tracking RNA expression. We also examine recent strategies for achieving spatiotemporal control of oligonucleotide function, particularly light-gated technologies that exploit the optical transparency of zebrafish embryos.
authors.affiliation: Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, California 94305, USA.
authors.forename: Ilya A
authors.initials: IA
authors.lastname: Shestopalov
authors.forename: James K
authors.initials: JK
authors.lastname: Chen
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental; D005821:Genetic Techniques; D009841:Oligonucleotides / Q000235:genetics*; D012313:RNA / Q000235:genetics; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development*
chemical_list: D009841:Oligonucleotides; D012313:RNA
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