title: Identification of quantitative trait loci for ABA responsiveness at the seedling stage associated with ABA-regulated gene expression in common wheat.
abstract: Responsiveness to abscisic acid (ABA) during vegetative growth plays an important role in regulating adaptive responses to various environmental conditions, including activation of a number of ABA-responsive genes. However, the relationship between gene expression and responsiveness to ABA at the seedling stage has not been well studied in wheat. In the present study, quantitative trait locus (QTL) analysis for ABA responsiveness at the seedling stage was performed using recombinant inbred lines derived from a cross between common wheat cultivars showing different ABA responsiveness. Five QTLs were found to be significant, located on chromosomes 1B, 2A, 3A, 6D and 7B. The QTL with the greatest effect was located on chromosome 6D and explained 11.12% of the variance in ABA responsiveness. The other QTLs each accounted for approximately 5-8% of the phenotypic variation. Expression analyses of three ABA-responsive Cor/Lea genes, Wdhn13, Wrab15 and Wrab17, showed that allelic differences in QTLs on chromosomes 2A, 6D and 7B influenced expression of these genes in seedlings treated with ABA. The 3A QTL appeared to be involved in the regulatory system of Wdhn13 and Wrab15, but not Wrab17. The effects of the 2A and 6D QTLs on gene expression were relatively large. The combination of alleles at the QTLs resulted in an additive or synergistic effect on Cor/Lea expression. These results indicate that the QTLs influencing ABA responsiveness are associated with ABA-regulated gene expression and suggest that the QTL on chromosome 6D with the largest effect acts as a key regulator of ABA responses including seedling growth arrest and gene expression during the vegetative stage.
authors.affiliation: Plant Genome Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Kobayashi
authors.forename: Shigeo
authors.initials: S
authors.lastname: Takumi
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Handa
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003080:Cold Temperature; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics; D005838:Genotype; D007178:Inbreeding; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D040641:Quantitative Trait Loci / Q000235:genetics*; D036226:Seedlings / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D014908:Triticum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*
chemical_list: D010940:Plant Proteins; D000040:Abscisic Acid
references.citation: Trends Plant Sci. 2009 Apr;14(4):229-36
references.pmid: 19285908
references.citation: Theor Appl Genet. 2003 Sep;107(5):806-13
references.pmid: 12838391
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:199-222
references.pmid: 15012233
references.citation: Theor Appl Genet. 2009 Nov;119(7):1223-35
references.pmid: 19669633
references.citation: Theor Appl Genet. 2005 Oct;111(6):1052-9
references.pmid: 16133317
references.citation: Theor Appl Genet. 2006 Apr;112(6):1042-51
references.pmid: 16450184
references.citation: Transgenic Res. 2008 Oct;17(5):755-67
references.pmid: 18034365
references.citation: Genome. 2008 Jun;51(6):426-32
references.pmid: 18521121
references.citation: Genomics. 1987 Oct;1(2):174-81
references.pmid: 3692487
references.citation: Theor Appl Genet. 2008 May;116(7):891-902
references.pmid: 18368385
references.citation: J Exp Bot. 2008;59(4):891-905
references.pmid: 18326864
references.citation: Physiol Plant. 2004 Apr;120(4):585-594
references.pmid: 15032820
references.citation: Funct Integr Genomics. 2007 Jan;7(1):53-68
references.pmid: 16775685
references.citation: Theor Appl Genet. 2005 Mar;110(5):865-80
references.pmid: 15719212
references.citation: Physiol Plant. 2008 Sep;134(1):74-86
references.pmid: 18433415
references.citation: Trends Plant Sci. 2007 Aug;12(8):343-51
references.pmid: 17629540
references.citation: Plant Physiol. 1987 May;84(1):61-6
references.pmid: 16665406
references.citation: Plant J. 2002 May;30(3):373-83
references.pmid: 12000684
references.citation: Theor Appl Genet. 2004 Oct;109(6):1105-14
references.pmid: 15490101
references.citation: Theor Appl Genet. 2005 Feb;110(3):550-60
references.pmid: 15655666
references.citation: J Exp Bot. 2001 Dec;52(365):2367-74
references.pmid: 11709586
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:571-599
references.pmid: 15012220
references.citation: Annu Rev Plant Biol. 2006;57:781-803
references.pmid: 16669782
references.citation: Nature. 2009 Jun 25;459(7250):1071-8
references.pmid: 19553990
references.citation: Theor Appl Genet. 2003 May;106(8):1491-6
references.pmid: 12750793
references.citation: J Plant Physiol. 2008 Feb;165(2):224-32
references.pmid: 17240477
references.citation: Genes Genet Syst. 2006 Apr;81(2):77-91
references.pmid: 16755132
references.citation: Plant Cell. 2002;14 Suppl:S15-45
references.pmid: 12045268
references.citation: Genome. 2000 Aug;43(4):689-97
references.pmid: 10984182
references.citation: Plant Cell. 2002;14 Suppl:S165-83
references.pmid: 12045276
references.citation: Theor Appl Genet. 2002 Aug;105(2-3):413-422
references.pmid: 12582546
references.citation: Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4782-7
references.pmid: 11287670
references.citation: Genes Genet Syst. 2005 Jun;80(3):185-97
references.pmid: 16172531
references.citation: Plant Physiol Biochem. 2008 Feb;46(2):205-11
references.pmid: 18061465
references.citation: Plant J. 2002 Nov;32(3):317-28
references.pmid: 12410810
references.citation: Genetics. 2008 Mar;178(3):1725-36
references.pmid: 18245824
references.citation: Genetics. 1998 Aug;149(4):2007-23
references.pmid: 9691054
references.citation: Theor Appl Genet. 2005 May;110(7):1315-23
references.pmid: 15803290
references.citation: Trends Plant Sci. 2007 Jul;12(7):301-9
references.pmid: 17573231
references.citation: Curr Opin Plant Biol. 2003 Oct;6(5):470-9
references.pmid: 12972048
references.citation: Theor Appl Genet. 2005 Nov;111(7):1357-64
references.pmid: 16133305
references.citation: Curr Opin Plant Biol. 2003 Oct;6(5):463-9
references.pmid: 12972047
references.citation: Theor Appl Genet. 1994 Nov;89(6):794-800
references.pmid: 24178027
references.citation: Theor Appl Genet. 2002 Nov;105(6-7):921-936
references.pmid: 12582918
references.citation: Theor Appl Genet. 2007 May;114(7):1129-39
references.pmid: 17387417
references.citation: Theor Appl Genet. 2002 May;104(6-7):1164-1172
references.pmid: 12582627
references.citation: Plant Mol Biol. 2002 Mar-Apr;48(5-6):649-65
references.pmid: 11999841