title: Improved tolerance toward fungal diseases in transgenic Cavendish banana (Musa spp. AAA group) cv. Grand Nain.
abstract: The most devastating disease currently threatening to destroy the banana industry worldwide is undoubtedly Sigatoka Leaf spot disease caused by Mycosphaerella fijiensis. In this study, we developed a transformation system for banana and expressed the endochitinase gene ThEn-42 from Trichoderma harzianum together with the grape stilbene synthase (StSy) gene in transgenic banana plants under the control of the 35S promoter and the inducible PR-10 promoter, respectively. The superoxide dismutase gene Cu,Zn-SOD from tomato, under control of the ubiquitin promoter, was added to this cassette to improve scavenging of free radicals generated during fungal attack. A 4-year field trial demonstrated several transgenic banana lines with improved tolerance to Sigatoka. As the genes conferring Sigatoka tolerance may have a wide range of anti-fungal activities we also inoculated the regenerated banana plants with Botrytis cinerea. The best transgenic lines exhibiting Sigatoka tolerance were also found to have tolerance to B. cinerea in laboratory assays.
authors.affiliation: Department of Fruit Tree Sciences, Institute of Plant Sciences, The Agricultural Research Organization, P.O. Box 6, 50250, Bet-Dagan, Israel.
authors.forename: Jane
authors.initials: J
authors.lastname: Vishnevetsky
authors.forename: Thomas L
authors.initials: TL
authors.lastname: White
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Palmateer
authors.forename: Moshe
authors.initials: M
authors.lastname: Flaishman
authors.forename: Yuval
authors.initials: Y
authors.lastname: Cohen
authors.forename: Yigal
authors.initials: Y
authors.lastname: Elad
authors.forename: Margarita
authors.initials: M
authors.lastname: Velcheva
authors.forename: Uri
authors.initials: U
authors.lastname: Hanania
authors.forename: Nachman
authors.initials: N
authors.lastname: Sahar
authors.forename: Oded
authors.initials: O
authors.lastname: Dgani
authors.forename: Avihai
authors.initials: A
authors.lastname: Perl
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D001203:Ascomycota / Q000145:classification / Q000472:pathogenicity*; D020171:Botrytis / Q000145:classification / Q000472:pathogenicity*; D002688:Chitinases / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000201:enzymology / Q000235:genetics; D028521:Musa / Q000201:enzymology* / Q000235:genetics* / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics; D027843:Vitis / Q000201:enzymology / Q000235:genetics
chemical_list: D013482:Superoxide Dismutase; D000217:Acyltransferases; C022674:stilbene synthase; D002688:Chitinases
references.citation: Phytochemistry. 2001 Sep;58(1):33-41
references.pmid: 11524110
references.citation: Transgenic Res. 1995 Jul;4(4):288-90
references.pmid: 7655517
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: Biotechniques. 1993 May;14(5):748-50
references.pmid: 8512694
references.citation: Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):7860-5
references.pmid: 9653105
references.citation: Transgenic Res. 1996 May;5(3):213-8
references.pmid: 8673150
references.citation: J Exp Bot. 2002 Feb;53(367):207-14
references.pmid: 11807124
references.citation: Plant Biotechnol J. 2005 Jan;3(1):57-69
references.pmid: 17168899
references.citation: Biotechnology (N Y). 1995 May;13(5):481-5
references.pmid: 9634789
references.citation: Transgenic Res. 2004 Apr;13(2):135-42
references.pmid: 15198201
references.citation: Plant Biotechnol J. 2003 Sep;1(5):321-36
references.pmid: 17166131
references.citation: Theor Appl Genet. 1993 Jan;85(5):568-76
references.pmid: 24195931
references.citation: Transgenic Res. 2001 Dec;10(6):533-43
references.pmid: 11817541
references.citation: Planta. 2003 Feb;216(4):587-96
references.pmid: 12569400
references.citation: Plant Mol Biol. 1994 Oct;26(2):667-77
references.pmid: 7948921
references.citation: Nature. 1993 Jan 14;361(6408):153-6
references.pmid: 8421520
references.citation: J Exp Bot. 2001 May;52(358):901-10
references.pmid: 11432907
references.citation: Planta. 2001 Feb;212(3):376-81
references.pmid: 11289602
references.citation: Plant Biotechnol J. 2009 Jan;7(1):2-12
references.pmid: 19021877
references.citation: Plant J. 1995 Jul;8(1):97-109
references.pmid: 7655510
references.citation: Plant Mol Biol. 1999 Apr;39(6):1221-30
references.pmid: 10380808
references.citation: Annu Rev Phytopathol. 2000 Sep;38:461-490
references.pmid: 11701851
references.citation: J Plant Physiol. 2006 Feb;163(2):147-53
references.pmid: 16399005
references.citation: Plant Cell Rep. 2003 Aug;21(12):1199-206
references.pmid: 12910370