title: Determination of lamotrigine in human serum by high-performance liquid chromatography–tandem mass spectrometry.
abstract: A rapid and sensitive liquid chromatography–tandem mass spectrometry method was developed and validated for quantification of lamotrigine in human serum. After a simple protein precipitation using acetonitrile, the analytes were separated on a Shideido 150 mm × 2.0 mm, 5 μm Capcell Pak C18 MG column using 70% acetonitrileas mobile phase at a flow rate of 200 μl/min. Lamotrigine was eluted at 1.98 min, ionized using electrospray ionization source, and then detected by multiple reaction monitoring mode. The precursor to product ion transitions of m/z 256.1–109.0 was used to quantify. The analytical measurement range is from 0.1 to 20 μg/ml and the upper clinical reportable range is chosen to be 100 μg/ml. The method was successfully employed in a clinical application.
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Woonhyoung
authors.initials: W
authors.lastname: Lee
authors.forename: Jeong-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Hyon-Suk
authors.initials: HS
authors.lastname: Kim
authors.forename: Oh Hun
authors.initials: OH
authors.lastname: Kwon
authors.forename: Byung In
authors.initials: BI
authors.lastname: Lee
authors.forename: Kyoung
authors.initials: K
authors.lastname: Heo
mesh_terms: D000927:Anticonvulsants / Q000097:blood / Q000493:pharmacokinetics; D018692:Antimanic Agents / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016903:Drug Monitoring / Q000379:methods; D004827:Epilepsy / Q000188:drug therapy; D006801:Humans; D000077213:Lamotrigine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014227:Triazines / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D000927:Anticonvulsants; D018692:Antimanic Agents; D014227:Triazines; D000077213:Lamotrigine
references.citation: Ther Drug Monit. 1999 Oct;21(5):559-66
references.pmid: 10519456
references.citation: J Chromatogr. 1991 Aug 21;554(1-2):181-9
references.pmid: 1795036
references.citation: Ther Drug Monit. 2006 Oct;28(5):603-7
references.pmid: 17038873
references.citation: J Pharm Biomed Anal. 1996 Apr;14(6):755-8
references.pmid: 8807551
references.citation: Ther Drug Monit. 1992 Dec;14(6):509-12
references.pmid: 1485374
references.citation: Eur J Clin Chem Clin Biochem. 1997 Oct;35(10):755-9
references.pmid: 9368793
references.citation: Chem Res Toxicol. 2000 Nov;13(11):1075-81
references.pmid: 11087428
references.citation: J Chromatogr B Biomed Sci Appl. 1997 Mar 28;691(1):139-44
references.pmid: 9140767
references.citation: Psychosomatics. 2005 Sep-Oct;46(5):464-94
references.pmid: 16145193
references.citation: J Chromatogr B Biomed Sci Appl. 1997 May 23;693(1):101-7
references.pmid: 9200523
references.citation: J Chromatogr Sci. 2002 Apr;40(4):219-23
references.pmid: 12004942
references.citation: Drug Metab Dispos. 1995 Mar;23(3):299-302
references.pmid: 7628292
references.citation: Br J Clin Pharmacol. 1997 Jan;43(1):23-7
references.pmid: 9056048
references.citation: Neurology. 2003 Aug 26;61(4):570-1
references.pmid: 12939444
references.citation: Ther Drug Monit. 2000 Oct;22(5):621-5
references.pmid: 11034270
references.citation: Ther Drug Monit. 1997 Aug;19(4):460-4
references.pmid: 9263389
references.citation: Epilepsy Res. 2001 Nov;47(1-2):151-4
references.pmid: 11673029
references.citation: Neurology. 2004 Jan 27;62(2):292-5
references.pmid: 14745072
references.citation: Electrophoresis. 2005 Feb;26(4-5):935-42
references.pmid: 15669009
references.citation: Ther Drug Monit. 1997 Dec;19(6):688-93
references.pmid: 9421112
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Mar 25;817(2):199-206
references.pmid: 15686986
references.citation: Epilepsia. 2000 Jun;41(6):709-13
references.pmid: 10840403
references.citation: Br J Clin Pharmacol. 2006 Feb;61(2):191-9
references.pmid: 16433873