Binding and release characteristics of insulin-like growth factor-1 from a collagen-glycosaminoglycan scaffold.
Tissue engineering is a promising technique for cartilage repair, but to optimize novel scaffolds before clinical trials, it is necessary to determine their characteristics for binding and release of growth factors. Toward this goal, a novel, porous collagen-glycosaminoglycan scaffold was loaded with a range of concentrations of insulin-like growth factor-1 (IGF-1) to evaluate its potential as a controlled delivery device. The kinetics of IGF-1 adsorption and release from the scaffold was demonstrated using radiolabeled IGF-1. Adsorption was rapid, and was approximately proportional to the loading concentration. Ionic bonding contributed to this interaction. IGF-1 release was studied over 14 days to compare the release profiles from different loading groups. Two distinct phases occurred: first, a burst release of up to 44% was noted within the first 24 h; then, a slow, sustained release (13%-16%) was observed from day 1 to 14. When the burst release was subtracted, the relative percentage of remaining IGF-1 released was similar for all loading groups and broadly followed t(½) kinetics until approximately day 6. Scaffold cross-linking using dehydrothermal treatment did not affect IGF-1 adsorption or release. Bioactivity of released IGF-1 was confirmed by seeding scaffolds (preadsorbed with unlabeled IGF-1) with human osteoarthritic chondrocytes and demonstrating increased proteoglycan production in vitro.
Mullen
Leanne M
LM
Department of Materials Science and Metallurgy, University of Cambridge, Cambridge, UK.
Best
Serena M
SM
Brooks
Roger A
RA
Ghose
Siddhartha
S
Gwynne
Jessica H
JH
Wardale
John
J
Rushton
Neil
N
Cameron
Ruth E
RE
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D015918:Infusion Pumps, Implantable; D007334:Insulin-Like Growth Factor I / Q000378:metabolism* / Q000493:pharmacokinetics*; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D011485:Protein Binding / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
D006025:Glycosaminoglycans; D007334:Insulin-Like Growth Factor I; D003094:Collagen
J Biomed Mater Res A. 2009 May;89(2):363-9
18431763
J Rheumatol. 2003 Jul;30(7):1565-70
12858460
Pediatr Res. 1989 Jan;25(1):76-82
2919122
J Orthop Res. 2000 Jan;18(1):68-77
10716281
J Control Release. 2000 Jul 3;67(2-3):385-94
10825569
J Orthop Res. 2001 Jan;19(1):26-32
11332617
Arch Biochem Biophys. 1995 Dec 1;324(1):173-88
7503553
Biomater Med Devices Artif Organs. 1983-1984;11(4):293-318
6091801
J Thorac Cardiovasc Surg. 2000 Jun;119(6):1162-8
10838533
J Biomed Mater Res. 2000 Mar 5;49(3):415-21
10602075
Am J Vet Res. 1997 May;58(5):524-30
9140562
Arch Biochem Biophys. 1995 Dec 1;324(1):159-72
7503552
Connect Tissue Res. 1995;31(3):189-95
15609626
J Control Release. 2004 Sep 30;99(2):281-92
15380637
Arch Biochem Biophys. 1988 Dec;267(2):416-25
3214163
J Control Release. 2014 Sep 28;190:31-2
25356469
Knee. 2005 Apr;12(2):113-9
15749446
Am J Vet Res. 2002 Feb;63(2):301-5
11843134
Ann Biomed Eng. 2004 Jan;32(1):26-34
14964719
Annu Rev Biomed Eng. 2002;4:109-28
12117753
Biomaterials. 1987 Mar;8(2):105-12
3580469
J Orthop Res. 2008 Apr;26(4):465-74
18050312
Osteoarthritis Cartilage. 2002 Aug;10(8):631-7
12479385
Biomaterials. 2004 Mar;25(6):1077-86
14615173
J Dermatol Sci. 2007 Sep;47(3):189-99
17507205
Biomaterials. 2002 Feb;23(4):1003-10
11791902
Anal Biochem. 1977 May 1;79(1-2):135-43
869171
J Orthop Res. 1999 Jul;17(4):475-87
10459752
Matrix. 1991 Feb;11(1):17-24
2027327
Proc Natl Acad Sci U S A. 1989 Feb;86(3):933-7
2915988
J Orthop Res. 1999 Jul;17(4):467-74
10459751
J Mater Sci Mater Med. 2008 Feb;19(2):567-75
18058201
Osteoarthritis Cartilage. 2006 May;14(5):403-12
16413799
J Orthop Res. 2004 Jan;22(1):140-4
14656672
J Control Release. 2005 Jan 3;101(1-3):111-25
15588898
J Orthop Res. 2000 Sep;18(5):781-9
11117301
J Endocrinol. 1990 Apr;125(1):67-74
2187051
Biochim Biophys Acta. 1986 Sep 4;883(2):173-7
3091074
Biomaterials. 2000 Aug;21(16):1689-99
10905410
J Orthop Res. 2001 Jul;19(4):738-49
11518286
Endocrinology. 1987 Sep;121(3):1070-5
3622376
Biomaterials. 1999 May;20(9):847-58
10226711
Biomaterials. 2001 Sep;22(17):2359-69
11511033
Osteoarthritis Cartilage. 1993 Apr;1(2):105-14
8886086
J Biomed Mater Res. 2002 Mar 5;59(3):422-8
11774299
Biomaterials. 2003 Aug;24(19):3255-63
12763453
Biomaterials. 2004 Feb;25(3):473-82
14585696
J R Soc Interface. 2006 Oct 22;3(10):589-601
16971328
J Control Release. 2008 Apr 7;127(1):12-21
18280603
Biomaterials. 1996 Apr;17(8):765-73
8730960
Growth Horm IGF Res. 2003 Apr-Jun;13(2-3):89-97
12735930
Biomaterials. 2004 Aug;25(18):4513-20
15046942
Adv Drug Deliv Rev. 2007 May 30;59(4-5):274-91
17544542
J Thorac Cardiovasc Surg. 1999 Aug;118(2):276-86
10425001
Biomaterials. 2005 Feb;26(4):433-41
15275817
Biochem J. 1989 Jun 1;260(2):543-8
2788408
Arthroscopy. 1996 Jun;12(3):300-8
8783824
Clin Orthop Relat Res. 2001 Oct;(391 Suppl):S271-9
11603711
J Orthop Res. 2001 Nov;19(6):1098-104
11781011