title: Crystallization and preliminary X-ray crystallographic analysis of a GroEL1 fragment from Mycobacterium tuberculosis H37Rv.
abstract: Full-length GroEL1 from Mycobacterium tuberculosis H37Rv was cloned, overexpressed and purified. Crystals were obtained by the hanging-drop vapor-diffusion method and contained a 23 kDa GroEL1 fragment. A complete native data set was collected from a single frozen crystal that belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 75.47, b = 78.67, c = 34.89 A, alpha = beta = gamma = 90 degrees , and diffracted to 2.2 A resolution on a home X-ray source.
authors.affiliation: Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston TX 77030, USA.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Sielaff
authors.forename: Ki Seog
authors.initials: KS
authors.lastname: Lee
authors.forename: Francis T F
authors.initials: FT
authors.lastname: Tsai
mesh_terms: D018834:Chaperonin 60 / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*
chemical_list: D018834:Chaperonin 60; D010446:Peptide Fragments
references.citation: J Bacteriol. 2004 Dec;186(23):8105-13
references.pmid: 15547284
references.citation: Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2608-12
references.pmid: 7681982
references.citation: Nature. 1999 Nov 11;402(6758):147-54
references.pmid: 10647006
references.citation: Methods Enzymol. 1997;276:307-26
references.pmid: 27754618
references.citation: J Mol Biol. 2005 Dec 9;354(4):940-51
references.pmid: 16288915
references.citation: Cell. 2005 Dec 2;123(5):861-73
references.pmid: 16325580
references.citation: Microbiology (Reading). 2002 Oct;148(Pt 10):3129-3138
references.pmid: 12368446
references.citation: Nature. 1997 Aug 21;388(6644):741-50
references.pmid: 9285585
references.citation: J Mol Biol. 1968 Apr 28;33(2):491-7
references.pmid: 5700707
references.citation: Nature. 1994 Oct 13;371(6498):578-86
references.pmid: 7935790
references.citation: J Bacteriol. 1989 Mar;171(3):1379-85
references.pmid: 2563997
references.citation: J Bacteriol. 2005 Feb;187(3):884-9
references.pmid: 15659666
references.citation: FEMS Microbiol Rev. 2009 Jul;33(4):785-800
references.pmid: 19416363
references.citation: FEMS Microbiol Lett. 2005 Aug 1;249(1):87-94
references.pmid: 16006064
references.citation: Nucleic Acids Res. 2009 Aug;37(15):4944-54
references.pmid: 19528065