Sclareol modulates the Treg intra-tumoral infiltrated cell and inhibits tumor growth in vivo.
A regulatory or suppressor T cell is functionally defined as a T cell that inhibits an immune response by influencing the activity of another cell type. On the other hand, Th1 cells express IFN-gamma and mediate cellular immunity. Sclareol exhibits growth inhibition and cytotoxic activity against a variety of human cancer cell lines. In the first set of experiments, Sclareol was isolated from the plant Salvia sclarea and our study assessed the immuno-therapeutic effectiveness of Sclareol by direct intra-tumoral injection. Secondly, several immunological parameters such as splenocytes proliferation, intra-tumor CD4+CD25+Foxp3+ Treg cells, IFN-gamma and IL-4 secretion and tumor size were assessed to evaluate the anti-tumoral immune response. By all means, the findings confirmed that the activity of Sclareol could reduce the tumor growth in vivo against breast cancer.
Noori
Shokoofe
S
Department of Biochemistry, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran. shnoori85@yahoo.com
Hassan
Zuhair M
ZM
Mohammadi
Mehdi
M
Habibi
Zohre
Z
Sohrabi
Nooshin
N
Bayanolhagh
Saeed
S
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D015552:Injections, Intralesional; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D012756:Sheep; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology
D000970:Antineoplastic Agents; D016207:Cytokines; D004224:Diterpenes; D015847:Interleukin-4; D007371:Interferon-gamma; C070760:sclareol