Sequence-specific recognition of methylated DNA by human zinc-finger proteins.
DNA methylation is an essential epigenetic mark. Three classes of mammalian proteins recognize methylated DNA: MBD proteins, SRA proteins and the zinc-finger proteins Kaiso, ZBTB4 and ZBTB38. The last three proteins can bind either methylated DNA or unmethylated consensus sequences; how this is achieved is largely unclear. Here, we report that the human zinc-finger proteins Kaiso, ZBTB4 and ZBTB38 can bind methylated DNA in a sequence-specific manner, and that they may use a mode of binding common to other zinc-finger proteins. This suggests that many other sequence-specific methyl binding proteins may exist.
Sasai
Nobuhiro
N
CNRS UMR7216, Université Paris-Diderot, Paris, France.
Nakao
Mitsuyoshi
M
Defossez
Pierre-Antoine
PA
D001483:Base Sequence; D001665:Binding Sites; D016384:Consensus Sequence; D019175:DNA Methylation; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C492254:ZBTB33 protein, human; C508975:ZBTB38 protein, human; C508974:ZBTB4 protein, human
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