title: Immunostaining of gastric cancer with neuroendocrine differentiation: Reg IV-positive neuroendocrine cells are associated with gastrin, serotonin, pancreatic polypeptide and somatostatin.
abstract: We previously reported that Reg IV is associated with neuroendocrine (NE) differentiation in gastric cancers. The aim was to examine which NE hormone products are related to Reg IV-positive NE cells and their roles in gastric cancers. In the present study, we performed immunohistochemical analysis in a tissue microarray (TMA) of a consecutive series of 630 cases with ten different antibodies, including chromogranin A, synaptophysin and neural cell adhesion molecule (NCAM) as NE differentiation markers, and gastrin, serotonin, calcitonin, gastrin-releasing peptide (GRP), pancreatic polypeptide (PP), somatostatin and glucagon as NE hormones. In 630 cases, we identified 205 (33%) with NE differentiation and 147 (23%) positive for Reg IV. Reg IV-positive cases showed NE differentiation more frequently than Reg IV-negative cases (P < 0.0001). In 205 cases with NE differentiation, Reg IV-positive cases expressed serotonin (P= 0.0032) and somatostatin (P= 0.036) more frequently than Reg IV-negative cases. Double immunofluorescence staining revealed co-expression of Reg IV with gastrin, serotonin and PP. These results indicate that Reg IV might be a mediating factor of several NE hormones.
authors.affiliation: Department of Molecular Pathology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sentani
authors.forename: Naohide
authors.initials: N
authors.lastname: Oue
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Noguchi
authors.forename: Naoya
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Keisuke
authors.initials: K
authors.lastname: Matsusaki
authors.forename: Wataru
authors.initials: W
authors.lastname: Yasui
mesh_terms: D002116:Calcitonin / Q000378:metabolism; D053379:Chromogranin A / Q000378:metabolism; D005755:Gastrins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000378:metabolism*; D009490:Neurosecretory Systems / Q000378:metabolism; D010191:Pancreatic Polypeptide / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D012701:Serotonin / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism*; D016708:Synaptophysin / Q000378:metabolism; D046888:Tissue Array Analysis
chemical_list: D053379:Chromogranin A; D005755:Gastrins; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; C428528:REG4 protein, human; D016708:Synaptophysin; D012701:Serotonin; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D002116:Calcitonin