title: Global analysis of modifications of the human BK virus structural proteins by LC-MS/MS.
abstract: BK virus, a human polyomavirus, may cause nephritis and urological disorders in patients who have undergone renal transplantation. Little is known about the characteristics of the BK viral proteins. In the current study, BK viral proteins were characterized by immunoblotting and LC-MS/MS. The results revealed that BK virus is composed of three structural proteins, VP1, VP2, and VP3 and four cellular histones, H2A, H2B, H3, and H4. The major structural protein, VP1, can be divided into 16 subspecies by two-dimensional gel electrophoresis. Modifications of VP1, VP2, and VP3 were comprehensively identified by LC-MS/MS. The presence of acetylation, cysteinylation, carboxymethylation, carboxyethylation, formylation, methylation, methylthiolation, oxidation, dioxidation, and phosphorylation could be identified. This is the first report providing an analysis of the global modifications present on polyomavirus structural proteins. The identification of these modifications of VP1, VP2, and VP3 should facilitate an understanding of the physiology of BKV during its life cycle.
authors.affiliation: Institute of Molecular Biology, National Chung Cheng University, Chia-Yi, Taiwan.
authors.forename: Chiung-Yao
authors.initials: CY
authors.lastname: Fang
authors.forename: Hsiang-Ying
authors.initials: HY
authors.lastname: Chen
authors.forename: Meilin
authors.initials: M
authors.lastname: Wang
authors.forename: Pei-Lain
authors.initials: PL
authors.lastname: Chen
authors.forename: Chi-Fang
authors.initials: CF
authors.lastname: Chang
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Chen
authors.forename: Cheng-Huang
authors.initials: CH
authors.lastname: Shen
authors.forename: Wei-Chih
authors.initials: WC
authors.lastname: Ou
authors.forename: Ming-Daw
authors.initials: MD
authors.lastname: Tsai
authors.forename: Pang-Hung
authors.initials: PH
authors.lastname: Hsu
authors.forename: Deching
authors.initials: D
authors.lastname: Chang
mesh_terms: D000818:Animals; D001739:BK Virus / Q000737:chemistry*; D002522:Chlorocebus aethiops; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D011499:Protein Processing, Post-Translational; D053719:Tandem Mass Spectrometry; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D014764:Viral Proteins