Characterisation of physico-mechanical properties and degradation potential of calcium alginate beads for use in embolisation.
High molecular weight alginate beads with 59% mannuronic acid content or 68% guluronic acid were prepared using a droplet generator and crosslinked in calcium chloride. The alginate beads were compared to current embolisation microspheres for compressibility and monitored over 12 weeks for size and weight change at 37 degrees C in low volumes of ringers solutions. A sheep uterine model was used to analyse bead degradation and inflammatory response over 12 weeks. Both the in vitro and in vivo data show good delivery, with a compressibility similar to current embolic beads. In vitro, swelling was noted almost immediately and after 12 weeks the first signs of degradation were noted. No difference was noted in vivo. This study has shown that high molecular weight alginate gel beads were well tolerated by the body, but beads associated with induced thrombi were susceptible to inflammatory cell infiltration. The beads were shown to be easy to handle and were still observable after 3 months in vivo. The beads were robust enough to be delivered through a 2.7 Fr microcatheter. This study has demonstrated that high molecular weight, high purity alginate bead can be considered as semi-permanent embolisation beads, with the potential to bioresorb over time.
Forster
Richard E J
RE
Biocompatibles UK Ltd., Farnham Business Park, Weydon Lane, Farnham, Surrey, GU9 8QL, UK.
Thürmer
Frank
F
Wallrapp
Christine
C
Lloyd
Andrew W
AW
Macfarlane
Wendy
W
Phillips
Gary J
GJ
Boutrand
Jean-Pierre
JP
Lewis
Andrew L
AL
D000464:Alginates / Q000737:chemistry*; D002122:Calcium Chloride / Q000737:chemistry; D004304:Dosage Forms; D004621:Embolization, Therapeutic; D005782:Gels; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry*; D008863:Microspheres
D000464:Alginates; D004304:Dosage Forms; D005782:Gels; D006603:Hexuronic Acids; C007896:guluronic acid; D020723:Glucuronic Acid; C008324:mannuronic acid; D002122:Calcium Chloride
Radiology. 2002 Aug;224(2):436-45
12147840
Biomaterials. 2002 Sep;23(18):3825-31
12164186
J Vasc Interv Radiol. 2008 Jan;19(1):47-57
18192467
Radiology. 1981 Aug;140(2):331-9
7196055
Obstet Gynecol. 2005 Jul;106(1):52-9
15994617
Biotechnol Bioeng. 1996 May 20;50(4):357-64
18626984
Lancet. 1995 Sep 9;346(8976):671-2
7544859
J Biomed Mater Res. 2001 Jan;54(1):76-86
11077405
J Appl Biomater. 1991 Winter;3(4):281-6
10147997
J Dent Res. 2003 Nov;82(11):903-8
14578503
J Vasc Interv Radiol. 2004 May;15(5):491-6
15126660
Obstet Gynecol. 2004 Feb;103(2):403-4
14754718
J Vasc Interv Radiol. 2009 Jul;20(7):936-45
19555888
Cardiovasc Intervent Radiol. 2009 Mar;32(2):303-12
19139955
Hoppe Seylers Z Physiol Chem. 1955 Dec 22;302(4-6):186-203
13331440
Cardiovasc Intervent Radiol. 2008 Jan-Feb;31(1):73-85
17943348
Am J Obstet Gynecol. 2007 Jun;196(6):519.e1-11
17547877
Neurosci Lett. 2009 Oct 9;463(3):176-81
19638295
J Control Release. 1999 Mar 8;58(1):21-8
10021486
Radiol Technol. 2007 Nov-Dec;79(2):157-78; quiz 179-82
18032751
J Mater Sci Mater Med. 2006 Dec;17(12):1193-204
17143749
Biotechnol Prog. 1990 Jan-Feb;6(1):51-3
1366434
N Engl J Med. 2007 Jan 25;356(4):360-70
17251532
Radiology. 2003 Feb;226(2):425-31
12563136
Int J Biol Macromol. 1997 Aug;21(1-2):47-55
9283015