Reduced CD27 expression on antigen-specific CD4+ T cells correlates with persistent active tuberculosis.
OBJECTIVE
CD27, a member of the tumor necrosis factor receptor family, has important role in generation of T cell immunity. In this study, association of CD27 expression on mycobacterial antigen-specific CD4+ T cells with pulmonary tuberculosis (TB) was investigated.


METHODS
Mycobacterial antigen-specific CD4+ T cells were identified based on CD154 expression and CD27 expression on antigen-specific CD4 T cells was analyzed by flow cytometry.


RESULTS
Compared with tuberculin-positive controls, patients with bacterial culture-positive pulmonary TB had significantly reduced CD27 expression on antigen-specific CD4 T cells. The persistent active TB patients had much lower percentages of CD27+ antigen-specific CD4 T cells than culture-positive new TB patients (P=0.008) and healthy controls (P=0.005). Logistic regression analysis on frequencies of CD27-expressing antigen-specific CD4 T cells and TB patients' clinical characteristics indicated that low percentage of CD27+ antigen-specific CD4 T cells correlated significantly with persistent active tuberculosis (P=0.002, odds ratio=19.6).


CONCLUSIONS
It is concluded that frequency of CD27+ antigen-specific CD4 T cells could be used as an immunological marker for persistent active TB.
Jiang
Jing
J
School of Nursing, Third Military Medical University, Chongqing, China.
Wang
Xianyuan
X
Wang
Xinjing
X
Cao
Zhihong
Z
Liu
Yanhua
Y
Dong
Mei
M
Tong
Aihua
A
Cheng
Xiaoxing
X
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009161:Mycobacterium / Q000276:immunology; D037182:T-Cell Antigen Receptor Specificity / Q000276:immunology*; D014373:Tuberculin / Q000032:analysis; D014376:Tuberculosis / Q000175:diagnosis* / Q000276:immunology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000032:analysis / Q000172:deficiency*; D055815:Young Adult
D015415:Biomarkers; D014373:Tuberculin; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
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