Physical, epidemiological, and molecular evaluation of infection by Cryptosporidium galli in Passeriformes.
Due to the scarcity of information related to the epidemiology of Cryptosporidium infection in passerine birds, this study aimed to determine the periodicity of fecal shedding of Cryptosporidium spp. oocysts, after natural infection, and its clinical signs, mortality, and molecular characterization. Four hundred eighty fecal samples were collected from 40 birds, including 372 samples from 31 adult birds and 108 samples from nine young birds (up to 12 months old), housed in five aviaries, monthly from September 2007 to September 2008, with the exception of April. The birds originated from aviaries in which the following species were raised: great-billed seed-finch (Oryzoborus maximiliani), lesser seed-finch (Oryzoborus angolensis), ultramarine grosbeak (Cyanocompsa brissonii), and rusty-collared seedeater (Sporophila collaris). The samples were preserved in 2.5% potassium dichromate at 4 degrees C until processing. The oocysts were purified by centrifugal flotation in Sheather's solution, followed by genomic DNA extraction and molecular characterization of oocysts using the nested polymerase chain reaction for amplification of fragments of the 18S subunit of rRNA gene. Intermittent shedding of oocysts was observed by positive amplification for Cryptosporidium spp. in 91 (24.5%) samples of adult birds and 14 (13%) of young birds. The sequencing of the amplified fragments enabled the identification of Cryptosporidium galli. Although all the aviaries had birds positive for C. galli, morbidity or mortality was observed in only one aviary and was associated with concomitant infection with Escherichia coli and Isospora sp.
da Silva
Deuvânia C
DC
Curso de Medicina Veterinária, UNESP-Univ Estadual Paulista, Campus de Araçatuba, Clóvis Pestana street, 793, CEP 16050-680, Bairro Dona Amélia, Araçatuba, SP, Brazil.
Homem
Camila G
CG
Nakamura
Alex A
AA
Teixeira
Weslen Fabrício P
WF
Perri
Sílvia Helena V
SH
Meireles
Marcelo V
MV
D000818:Animals; D001715:Bird Diseases / Q000453:epidemiology* / Q000401:mortality / Q000469:parasitology / Q000473:pathology*; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000469:parasitology; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D046109:Passeriformes / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S
J Infect Dis. 2001 Feb 1;183(3):492-7
11133382
Vet Parasitol. 2000 Feb 1;87(4):261-79
10669097
J Am Vet Med Assoc. 1986 Nov 1;189(9):1126-8
3505949
Appl Environ Microbiol. 2000 Dec;66(12):5492-8
11097935
Vet Res. 1996;27(2):185-92
8721298
Int J Parasitol. 2008 Sep;38(11):1239-55
18479685
Appl Environ Microbiol. 2006 Dec;72(12):7548-53
17028234
J Protozool. 1986 May;33(2):289-96
3735157
J Comp Pathol. 1955 Jul;65(3):262-6
13242675
J Eukaryot Microbiol. 2003;50 Suppl:531-3
14736153
J Parasitol. 2006 Jun;92(3):623-6
16884009
Avian Pathol. 2007 Aug;36(4):275-8
17620172
J Protozool. 1991 Nov-Dec;38(6):180S-181S
1818158
Vet Parasitol. 2008 May 31;153(3-4):363-7
18343038
J Parasitol. 2003 Aug;89(4):809-13
14533694
J Clin Microbiol. 2000 Nov;38(11):3984-90
11060056
Avian Dis. 2008 Dec;52(4):702-5
19166068
Appl Environ Microbiol. 2004 Jan;70(1):452-8
14711674
Exp Parasitol. 2010 Jan;124(1):113-20
19545515
Vet Pathol. 1977 Nov;14(6):606-17
929895
Clin Microbiol Rev. 2004 Jan;17(1):72-97
14726456
Parasitology. 2001;122 Suppl:S23-38
11442193
J Clin Microbiol. 1990 Mar;28(3):495-503
1691208
Avian Dis. 1990 Oct-Dec;34(4):1027-30
2282009
Vet Parasitol. 2009 Dec 3;166(1-2):47-51
19683397
J Eukaryot Microbiol. 2000 Jan-Feb;47(1):91-5
10651302
Poult Sci. 2000 Apr;79(4):514-8
10780646
Int J Parasitol. 2001 Mar;31(3):289-96
11226456
J Parasitol. 1994 Oct;80(5):823-6
7931919
Appl Environ Microbiol. 2004 Jul;70(7):4211-5
15240303
J Vet Med A Physiol Pathol Clin Med. 2006 Aug;53(6):282-5
16901269
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
9396791