High throughput and wide field of view EUV microscope for blur-free one-shot imaging of living organisms.
We present and demonstrate the use of an extreme ultraviolet (EUV) microscope that was developed in-house. Images are acquired using Bragg reflection multilayer optics and a laser-produced plasma light source. The upper-limit spatial resolution of the EUV microscope is 130 nm with a 10 ns exposure time and 250 x 250 microm(2) field of view. Resolution is superior to that of visible microscopes with the same size of field of view, and the exposure time is short enough to observe fine structures in-vivo. Observation of the cerebral cortex of a mouse is demonstrated.
Ejima
Takeo
T
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1, Katahira, Aoba-ku, Sendai, 980-8577, Japan. ejima@tagen.tohoku.ac.jp
Ishida
Fumihiko
F
Murata
Hiromichi
H
Toyoda
Mitsunori
M
Harada
Tetsuo
T
Tsuru
Toshihide
T
Hatano
Tadashi
T
Yanagihara
Mihiro
M
Yamamoto
Masaki
M
Mizutani
Haruo
H
D000818:Animals; D002540:Cerebral Cortex / Q000473:pathology; D004577:Electron Probe Microanalysis / Q000379:methods*; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008853:Microscopy / Q000379:methods*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D055095:Optics and Photonics; D011108:Polymers / Q000737:chemistry; D013696:Temperature; D014466:Ultraviolet Rays
D011108:Polymers