title: Benchtop chemistry for the rapid prototyping of label-free biosensors: Transmission localized surface plasmon resonance platforms.
abstract: Herein, a simple label-free biosensor fabrication method is demonstrated based on transmission localized surface plasmon resonance (T-LSPR). The platform, which consists of a silver nanoparticle array, can be prepared in just a few minutes using benchtop chemistry. The array was made by a templating technique in conjunction with the photoreduction of Ag ions from solution. This metal surface was functionalized with biotin-linked thiol ligands for binding streptavidin molecules from solution. For an array of 19 nm diameter silver nanoparticles, a redshift in the T-LSPR spectrum of 24 nm was observed upon protein-ligand binding at saturation. The binding constant was found to be 2x10(12) M(-1). Platforms were also fabricated with silver nanoparticles of 34, 55, and 72 nm diameters. The maximum LSPR wavelength shift was nanoparticle size dependent and the maximum sensitivity was obtained with the smaller nanoparticles.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Wei-Ssu
authors.initials: WS
authors.lastname: Liao
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Tinglu
authors.initials: T
authors.lastname: Yang
authors.forename: Edward T
authors.initials: ET
authors.lastname: Castellana
authors.forename: Jixin
authors.initials: J
authors.lastname: Chen
authors.forename: Paul S
authors.initials: PS
authors.lastname: Cremer
references.citation: Anal Chem. 2008 Mar 15;80(6):2244-9
references.pmid: 18288819
references.citation: Anal Chem. 2003 Dec 15;75(24):6894-900
references.pmid: 14670050
references.citation: J Am Chem Soc. 2002 Sep 4;124(35):10596-604
references.pmid: 12197762
references.citation: J Phys Chem B. 2004 Sep 16;108(37):13957-13962
references.pmid: 36168191
references.citation: J Am Chem Soc. 2005 Dec 14;127(49):17453-9
references.pmid: 16332097
references.citation: Opt Lett. 2000 Mar 15;25(6):372-4
references.pmid: 18059883
references.citation: Anal Chem. 2007 Jul 15;79(14):5278-83
references.pmid: 17567106
references.citation: Photochem Photobiol Sci. 2004 Jan;3(1):142-4
references.pmid: 14768632
references.citation: Chemistry. 2005 Sep 19;11(19):5555-62
references.pmid: 16007692
references.citation: Biomaterials. 2000 Sep;21(18):1823-35
references.pmid: 10919686
references.citation: J Phys Chem B. 2005 Sep 22;109(37):17347-9
references.pmid: 16853216
references.citation: J Am Chem Soc. 2005 Apr 13;127(14):5043-8
references.pmid: 15810838
references.citation: Science. 2001 Nov 30;294(5548):1901-3
references.pmid: 11729310
references.citation: Nano Lett. 2006 Sep;6(9):2060-5
references.pmid: 16968025
references.citation: J Am Chem Soc. 2001 Apr 4;123(13):3177-8
references.pmid: 11457046
references.citation: J Am Chem Soc. 2006 Aug 2;128(30):9590-1
references.pmid: 16866487
references.citation: Anal Chem. 2004 Apr 1;76(7):2071-82
references.pmid: 15053673
references.citation: J Am Chem Soc. 2004 Dec 15;126(49):15950-1
references.pmid: 15584714
references.citation: J Am Chem Soc. 2003 May 21;125(20):6140-8
references.pmid: 12785845
references.citation: ACS Nano. 2008 Apr;2(4):687-92
references.pmid: 19206599
references.citation: Anal Chem. 2002 Feb 1;74(3):504-9
references.pmid: 11838667
references.citation: Anal Chem. 2001 Jan 1;73(1):1-7
references.pmid: 11195491
references.citation: J Am Chem Soc. 2007 Jan 10;129(1):84-92
references.pmid: 17199286
references.citation: ACS Nano. 2009 Apr 28;3(4):795-806
references.pmid: 19296619
references.citation: Anal Bioanal Chem. 2003 Oct;377(3):528-39
references.pmid: 12879189
references.citation: Chem Commun (Camb). 2002 Sep 7;(17):1910-1
references.pmid: 12271670
references.citation: Biointerphases. 2008 Sep;3(3):FD30-40
references.pmid: 20408698
references.citation: J Am Chem Soc. 2005 Sep 14;127(36):12444-5
references.pmid: 16144364
references.citation: Anal Chem. 2006 Jan 1;78(1):107-12
references.pmid: 16383316
references.citation: Anal Chem. 2004 Sep 15;76(18):5370-8
references.pmid: 15362894
references.citation: Nature. 2003 Oct 2;425(6957):487-90
references.pmid: 14523440
references.citation: Langmuir. 2004 Jun 8;20(12):4813-5
references.pmid: 15984236
references.citation: Adv Protein Chem. 1975;29:85-133
references.pmid: 237414
references.citation: Chemistry. 2002 Sep 2;8(17):3849-57
references.pmid: 12203279
references.citation: Anal Chem. 2008 Feb 15;80(4):984-9
references.pmid: 18197636
references.citation: J Phys Chem B. 2006 Apr 27;110(16):8307-13
references.pmid: 16623513