The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo.
The aim of this study was to investigate the odontogenic differentiation of human dental pulp stem cells (DPSCs) on nanofibrous (NF)-poly(l-lactic acid) (PLLA) scaffolds in vitro and in vivo. Highly porous NF-PLLA scaffolds which mimic the architecture of collagen type I fibers were fabricated by the combination of a phase-separation technique and a porogen-leaching method. The human DPSCs were then seeded onto the scaffolds and cultured in different media for odontogenic differentiation: "Control" medium without supplements; "DXM" medium containing 10(-8)M dexamethasone (DXM), 50 microgml(-1) ascorbic acid and 5mM beta-glycerophosphate; "BMP-7+DXM" medium containing 10(-8)M DXM, 50 microgml(-1) ascorbic acid, 5mM beta-glycerophosphate plus 50 ngml(-1) bone morphogenetic protein 7 (BMP-7). For odontogenic differentiation study in vitro, alkaline phosphatase activity quantification, reverse transcription polymerase chain reaction, scanning electron microscopy, von Kossa staining and calcium content quantification were carried out. While both "DXM" medium and "BMP-7+DXM" medium induced the DPSCs to odontoblast-like cells, the "BMP-7+DXM" medium had greater inducing capacity than the "DXM" medium. Consistent with the in vitro studies, the "BMP-7+DXM" group presented more extracellular matrix and hard tissue formation than the "DXM" group after 8 weeks of ectopic implantation in nude mice. Differentiation of DPSCs into odontoblast-like cells was identified by the positive immunohistochemical staining for dentin sialoprotein. In conclusion, odontogenic differentiation of DPSCs can be achieved on NF-PLLA scaffolds both in vitro and in vivo; the combination of BMP-7 and DXM induced the odontogenic differentiation more effectively than DXM alone. The NF-PLLA scaffold and the combined odontogenic inductive factors provide excellent environment for DPSCs to regenerate dental pulp and dentin.
Wang
Jing
J
Department of Operative Dentistry and Endodontics, Fourth Military Medical University, Xi'an, People's Republic of China.
Liu
Xiaohua
X
Jin
Xiaobing
X
Ma
Haiyun
H
Hu
Jiang
J
Ni
Longxing
L
Ma
Peter X
PX
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D055419:Bone Morphogenetic Protein 7 / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D017690:Cell Transplantation; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D008819:Mice, Nude; D057139:Nanofibers; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D055419:Bone Morphogenetic Protein 7; D003470:Culture Media; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)
J Dent Res. 2002 Aug;81(8):531-5
12147742
J Orthop Res. 2008 Nov;26(11):1440-8
18404732
J Endod. 1997 Jun;23(6):378-82
9545947
Tissue Eng. 2005 Mar-Apr;11(3-4):357-68
15869416
Oral Dis. 2007 Mar;13(2):151-7
17305615
J Dent Res. 2004 Jul;83(7):523-8
15218040
Biomaterials. 2004 May;25(11):2065-73
14741621
J Oral Rehabil. 2007 Dec;34(12):872-906; discussion 940
18034671
Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13625-30
11087820
Biomaterials. 2006 Nov;27(33):5658-68
16916542
Tissue Eng. 2001 Feb;7(1):23-33
11224921
Eur J Oral Sci. 2001 Dec;109(6):422-4
11767280
J Endod. 2005 Oct;31(10):711-8
16186748
Injury. 2008 Dec;39(12):1391-402
19027898
Int J Dev Biol. 1995 Feb;39(1):273-80
7626417
Eur J Oral Sci. 2006 Aug;114(4):310-7
16911102
Adv Drug Deliv Rev. 2008 Jan 14;60(2):184-98
18045729
Eur J Endocrinol. 2000 Jan;142(1):9-21
10633215
Biomaterials. 2009 May;30(13):2516-22
19176243
Quintessence Int. 2008 Feb;39(2):117-29
18560650
Anat Rec. 1989 Jun;224(2):154-66
2672882
Nat Biotechnol. 2003 Sep;21(9):1025-32
12949568
Arch Oral Biol. 1994 Oct;39(10):833-8
7741652
Biomaterials. 2006 Jul;27(21):3973-9
16564086
Clin Orthop Relat Res. 2009 Dec;467(12):3239-48
19396502
J Biol Chem. 1998 Apr 17;273(16):9457-64
9545272
Biomaterials. 2008 Oct;29(28):3815-21
18617260
J Cell Physiol. 2006 Mar;206(3):693-701
16222704
J Biomed Mater Res A. 2003 Nov 1;67(2):531-7
14566795
Arch Oral Biol. 2000 Feb;45(2):173-7
10716622
J Dent Res. 2003 Oct;82(10):776-80
14514755
Biomaterials. 2007 Jan;28(2):335-43
16854461
Tissue Eng. 2005 Sep-Oct;11(9-10):1599-610
16259613
J Biomed Mater Res. 2002 Dec 5;62(3):350-8
12209920
J Endod. 2008 Apr;34(4):421-6
18358888
Biomaterials. 2006 Jun;27(17):3238-48
16504285
Tissue Eng Part A. 2009 Jul;15(7):1855-64
19196152
Tissue Eng Part A. 2008 Feb;14(2):285-94
18333781
Tissue Eng Part A. 2009 Feb;15(2):367-75
18652538
Tissue Eng Part A. 2009 Aug;15(8):2155-62
19348597
PLoS Med. 2007 Feb;4(2):e9
17311467
J Biomed Mater Res. 1999 Jul;46(1):60-72
10357136
J Cell Biochem. 2002;86(2):348-56
12112004
Quintessence Int. 2007 Oct;38(9):733-43
17873980
Steroids. 2004 Apr;69(4):219-26
15183687