title: Role of ATP-hydrolysis in the dynamics of a single actin filament.
abstract: We study the stochastic dynamics of growth and shrinkage of single actin filaments taking into account insertion, removal, and ATP hydrolysis of subunits either according to the vectorial mechanism or to the random mechanism. In a previous work, we developed a model for a single actin or microtubule filament where hydrolysis occurred according to the vectorial mechanism: the filament could grow only from one end, and was in contact with a reservoir of monomers. Here we extend this approach in two ways--by including the dynamics of both ends and by comparing two possible mechanisms of ATP hydrolysis. Our emphasis is mainly on two possible limiting models for the mechanism of hydrolysis within a single filament, namely the vectorial or the random model. We propose a set of experiments to test the nature of the precise mechanism of hydrolysis within actin filaments.
authors.affiliation: Physico-Chimie UMR 168, Institut Curie, Paris, France. ranjithp@iitb.ac.in
authors.forename: Padinhateeri
authors.initials: P
authors.lastname: Ranjith
authors.forename: Kirone
authors.initials: K
authors.lastname: Mallick
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Joanny
authors.forename: David
authors.initials: D
authors.lastname: Lacoste
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D004058:Diffusion; D006868:Hydrolysis; D008954:Models, Biological; D013997:Time Factors
chemical_list: D000199:Actins; D000255:Adenosine Triphosphate
references.citation: J Biol Chem. 1986 Aug 15;261(23):10785-92
references.pmid: 2942544
references.citation: Biophys J. 2009 Mar 18;96(6):2146-59
references.pmid: 19289041
references.citation: Biochemistry. 1996 Sep 17;35(37):12038-45
references.pmid: 8810908
references.citation: Biophys J. 2005 Feb;88(2):1387-402
references.pmid: 15556992
references.citation: Biophys J. 2008 Aug;95(3):1050-62
references.pmid: 18390612
references.citation: Phys Rev E Stat Nonlin Soft Matter Phys. 2007 Oct;76(4 Pt 1):041907
references.pmid: 17995026
references.citation: J Stat Mech. 2007 May 1;(5):L05004
references.pmid: 18594695
references.citation: Biochemistry. 1986 Aug 26;25(17):4899-906
references.pmid: 2945593
references.citation: Biophys J. 2001 Jan;80(1):149-54
references.pmid: 11159390
references.citation: Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 1996 Nov;54(5):5538-5560
references.pmid: 9965740
references.citation: Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8543-8
references.pmid: 15939882
references.citation: Phys Rev Lett. 2009 Jul 24;103(4):048102
references.pmid: 19659403
references.citation: Annu Rev Biophys Biomol Struct. 2000;29:545-76
references.pmid: 10940259
references.citation: Nat Cell Biol. 2002 Sep;4(9):666-73
references.pmid: 12198494
references.citation: Phys Rev Lett. 1993 Mar 1;70(9):1347-1350
references.pmid: 10054353
references.citation: J Cell Biol. 1986 Dec;103(6 Pt 2):2747-54
references.pmid: 3793756
references.citation: Phys Biol. 2006 Mar 31;3(1):83-92
references.pmid: 16582473
references.citation: Proc Natl Acad Sci U S A. 2007 May 22;104(21):8827-32
references.pmid: 17517656
references.citation: Phys Biol. 2008 Jul 14;5(3):036002
references.pmid: 18626129
references.citation: Biophys J. 2006 Apr 15;90(8):2673-85
references.pmid: 16443647
references.citation: Science. 2008 Nov 28;322(5906):1353-6
references.pmid: 18927356
references.citation: Proc Natl Acad Sci U S A. 1985 Nov;82(21):7207-11
references.pmid: 3864156
references.citation: Biochemistry. 1996 Apr 9;35(14):4396-402
references.pmid: 8605188
references.citation: Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16531-6
references.pmid: 18931306
references.citation: J Theor Biol. 2008 May 7;252(1):173-83
references.pmid: 18279896
references.citation: Biochemistry. 1986 Dec 2;25(24):7789-92
references.pmid: 3801442
references.citation: Biophys J. 2004 May;86(5):2720-39
references.pmid: 15111391