Interaction of galactoglucomannan oligosaccharides with auxin in mung bean primary root.
In the present paper timing of galactoglucomannan oligosaccharides (GGMOs) with exogenously added indole-3-butyric acid (IBA) action on early germination stage (24 h) and primary root elongation of mung bean (Vigna radiata (L.) Wilczek) has been studied. GGMOs inhibited primary root elongation induced by low concentration (10(-8) M) of IBA. This inhibition was considerably higher after preincubation with GGMOs compared with other timing experiments. The most intensive inhibition of elongation has been ascertained at the 10(-8) M concentration of GGMOs. On the other hand GGMOs stimulated this elongation inhibited by high IBA concentration (10(-4) M). This stimulation was the most intensive by simultaneous addition of IBA and GGMOs at the beginning of the experiment and subsequent seeds incubation in distilled water. Our results indicate competition between GGMOs and auxin. The root growth inhibition, induced by GGMOs and/or IBA, was accompanied by the increase of cell wall-associated peroxidase activity and by a higher number of peroxidase isoenzymes. The presence of different peroxidase isoenzymes in experiments with distinct treatment of GGMOs and IBA could indicate variations in the mechanism of interaction between GGMOs and IBA.
Kollárová
Karin
K
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia. Karin.Kollarova@savba.sk <Karin.Kollarova@savba.sk>
Vatehová
Zuzana
Z
Slováková
L'udmila
L
Lisková
Desana
D
D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D007887:Fabaceae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018525:Germination / Q000187:drug effects*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology; D008351:Mannans / Q000494:pharmacology*; D009844:Oligosaccharides / Q000494:pharmacology; D010544:Peroxidases / Q000378:metabolism; D028222:Picea / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development; D012639:Seeds / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
D007210:Indoleacetic Acids; D007211:Indoles; D008351:Mannans; D009844:Oligosaccharides; D010936:Plant Extracts; D010937:Plant Growth Regulators; C443804:galactoglucomannan; C014612:indolebutyric acid; D010544:Peroxidases