title: Dual binding mode of the nascent polypeptide-associated complex reveals a novel universal adapter site on the ribosome.
abstract: Nascent polypeptide-associated complex (NAC) was identified in eukaryotes as the first cytosolic factor that contacts the nascent polypeptide chain emerging from the ribosome. NAC is present as a homodimer in archaea and as a highly conserved heterodimer in eukaryotes. Mutations in NAC cause severe embryonically lethal phenotypes in mice, Drosophila melanogaster, and Caenorhabditis elegans. In the yeast Saccharomyces cerevisiae NAC is quantitatively associated with ribosomes. Here we show that NAC contacts several ribosomal proteins. The N terminus of betaNAC, however, specifically contacts near the tunnel exit ribosomal protein Rpl31, which is unique to eukaryotes and archaea. Moreover, the first 23 amino acids of betaNAC are sufficient to direct an otherwise non-associated protein to the ribosome. In contrast, alphaNAC (Egd2p) contacts Rpl17, the direct neighbor of Rpl31 at the ribosomal tunnel exit site. Rpl31 was also recently identified as a contact site for the SRP receptor and the ribosome-associated complex. Furthermore, in Escherichia coli peptide deformylase (PDF) interacts with the corresponding surface area on the eubacterial ribosome. In addition to the previously identified universal adapter site represented by Rpl25/Rpl35, we therefore refer to Rpl31/Rpl17 as a novel universal docking site for ribosome-associated factors on the eukaryotic ribosome.
authors.affiliation: Department of Biochemistry, Ludwig-Maximilians-Universität München, Gene Center Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Pech
authors.forename: Thomas
authors.initials: T
authors.lastname: Spreter
authors.forename: Roland
authors.initials: R
authors.lastname: Beckmann
authors.forename: Birgitta
authors.initials: B
authors.lastname: Beatrix
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D018833:Chaperonins / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D051379:Mice; D009154:Mutation; D010455:Peptides / Q000737:chemistry*; D010641:Phenotype; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D012269:Ribosomal Proteins / Q000737:chemistry; D012270:Ribosomes / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D003432:Cross-Linking Reagents; D010455:Peptides; D012269:Ribosomal Proteins; C032493:ribosomal protein L17; C041672:ribosomal protein L34; D018833:Chaperonins
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