title: Application of bipolar electrodialysis to E. coli fermentation for simultaneous acetate removal and pH control.
abstract: The application of bipolar electrodialysis (BPED) for the simultaneous removal of inhibitory acetate and pH control during E. coli fermentation was investigated. A two cell pair electrodialysis module, consisting of cation exchange, anion exchange and bipolar membranes with working area of 100 cm(2) each, was integrated with a standard 7 l stirred tank bioreactor. Results showed that BPED was beneficial in terms of in situ removal of inhibitory acetate and a reduction in the amount NH(4)OH used for pH control. In batch and fed-batch BPED fermentations, base additions were decreased by up to 50% in both cases compared to electrodialysis (ED) fermentations with pH controlled at 6.7 +/- 0.1. Consequently, the final biomass (34.2 g DCW l(-1)) and recombinant protein (5.5 g l(-1)) concentrations obtained were increased by up to 37 and 20%, respectively.
authors.affiliation: Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Wong
authors.forename: John M
authors.initials: JM
authors.lastname: Woodley
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Lye
mesh_terms: D000085:Acetates / Q000737:chemistry*; D000641:Ammonia / Q000737:chemistry / Q000633:toxicity; D019149:Bioreactors; D003470:Culture Media; D003956:Dialysis / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration
chemical_list: D000085:Acetates; D003470:Culture Media; D000641:Ammonia