Comparison between multiplex PCR and phenotypic systems for Candida spp. identification.
This study evaluated the performances of three phenotypic systems (RapID Yeast panel, Vitek2 YST card, and API 20 C AUX) and multiplex PCR for Candida spp. identification. Four-hundred and fifty clinical strains of Candida spp. were identified with the four systems and results of multiplex PCR were compared with those of phenotypic methods. The best correspondence was obtained between Multiplex PCR and API 20 C AUX (83.7%), but the other comparisons showed similar values (81.7% and 79.3% for Vitek2 and RapID Yeast panel respectively). The correspondence was lower for all the methods in identification of C. krusei; this may be of concern in addition to the azole resistance and the often endogenous origin of this yeast. In the comparison with the three phenotypic methods, multiplex PCR could be reliable and time-saving in the identification of Candida spp. for diagnostics purposes. Nowadays, a large variety of both traditional and molecular methods for Candida spp. identification are commercially available. Multiplex PCR applied in this study may be more rapid and sensitive than phenotypic systems, and less expensive than other molecular methods.
Liguori
Giorgio
G
Chair of Hygiene and Epidemiology, Department of Studies of Institutions and Territorial Systems, "Parthenope" University of Naples, Naples, Italy. giorgio.liguori@uniparthenope.it
Gallé
Francesca
F
Lucariello
Angela
A
Di Onofrio
Valeria
V
Albano
Luciana
L
Mazzarella
Gennaro
G
D'Amora
Maurizio
M
Rossano
Fabio
F
D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D005656:Fungal Proteins / Q000235:genetics; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*
D005656:Fungal Proteins