Tongue muscle-derived stem cells express connexin 43 and improve cardiac remodeling and survival after myocardial infarction in mice.
BACKGROUND
Cell transplantation therapy for heart failure is hindered by poor differentiation into cardiomyocytes and arrhythmias caused by the poor expression of connexin 43 (Cx43). A new stem cell source for cardiac regeneration is needed.


RESULTS
Tongue muscle-derived Sca-1(+) cells (TDSCs) were isolated from normal and green fluorescence protein (GFP)-transgenic mouse tongues using surface antigen Sca-1. Cardiomyogenic differentiation was confirmed by measuring the calcium transient and the expression of cardiac-specific genes. The formation of gap junctions was confirmed by the expression of Cx43 and the dye transfer method. The contraction of regenerated cells was demonstrated by the calcium transients. GFP mouse-derived TDSCs were transplanted into hearts in a model of acute myocardial infarction. Three months after transplantation, LV remodeling was attenuated and the survival rate was improved compared with the control group.


CONCLUSIONS
TDSCs form gap junctions and improve cardiac function and long-term survival after myocardial infarction.
Shibuya
Masaki
M
Division of Cardiology, Department of Medicine and Clinical Science, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Miura
Toshiro
T
Fukagawa
Yasuhiro
Y
Akashi
Shintaro
S
Oda
Takamasa
T
Kawamura
Shuji
S
Ikeda
Yasuhiro
Y
Matsuzaki
Masunori
M
D000818:Animals; D002454:Cell Differentiation; D018031:Connexin 43 / Q000032:analysis*; D017629:Gap Junctions; D049452:Green Fluorescent Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009203:Myocardial Infarction / Q000401:mortality / Q000628:therapy*; D009206:Myocardium / Q000166:cytology; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology; D012038:Regeneration; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*; D015996:Survival Rate; D014059:Tongue / Q000166:cytology*; D020257:Ventricular Remodeling
D018031:Connexin 43; D049452:Green Fluorescent Proteins