Molecular and serological survey on taeniasis and cysticercosis in Kanchanaburi Province, Thailand.
A community-based field survey on taeniasis and cysticercosis was performed in two villages in Thong Pha Phum District, Kanchanaburi Province, central Thailand, where 3 Taenia species, T. solium, T. saginata and T. asiatica, are sympatrically occurring. Four (0.6%) out of 667 stool samples were egg-positive for Taenia sp. by Kato-Katz technique. Three out of those four persons and other three persons who were Taenia egg-negative but having a recent (<1 year) history of discharging worms in stool were treated with niclosamide. One Taenia egg-positive woman was not treated because of severe ascites. After treatment, three persons expelled long strobilae with scolices and two persons expelled strobilae without scolex. One Taenia egg-positive person did not expel any worms post-treatment. Among 5 persons, four expelled a single worm, whereas one expelled multiple worms, may be 6 worms but not confirmed by detection of scolices. One scolex was armed with hooklets, whereas 2 others did not. Multiplex PCR of 10 expelled proglottids (including 6 estimated worms from one patient) revealed that one sample was T. solium, one T. saginata, and 8 T. asiatica. A total of 159 residents agreed to receive a serological test for cysticercosis. By ELISA using partially purified glycoprotein antigen, 9 cases, 5 and 4 from villages A and B respectively, were found to be sero-positive. The five and an additional sample on the border line from village A were evaluated using confirmative immunoblot using recombinant chimeric antigen. Among the six samples, four including the border line sample were confirmed to be cysticercosis by immunoblotting. One of the 4 persons had neurological symptoms with nodular lesions in the brain by computed tomography. These 4 confirmed or suspected cysticercosis cases were free of T. solium worms, but two of them including confirmed NCC case had a past (>1 year) history of expelling proglottids in the stool.
Anantaphruti
Malinee T
MT
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. tmmtr@mahidol.ac.th
Okamoto
Munehiro
M
Yoonuan
Tippayarat
T
Saguankiat
Surapol
S
Kusolsuk
Teera
T
Sato
Megumi
M
Sato
Marcello O
MO
Sako
Yasuhito
Y
Waikagul
Jitra
J
Ito
Akira
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D002648:Child; D003551:Cysticercosis / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D018509:DNA, Helminth / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction / Q000379:methods; D012424:Rural Population; D013621:Taenia / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D013622:Taeniasis / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D013785:Thailand; D055815:Young Adult
D000909:Antibodies, Helminth; D018509:DNA, Helminth