Bladder regeneration by collagen scaffolds with collagen binding human basic fibroblast growth factor.
OBJECTIVE
Studies show that basic fibroblast growth factor can promote bladder regeneration. However, the lack of targeting delivery approaches limits its clinical application. We investigated a collagen based targeting system for bladder regeneration. A collagen binding domain was added to the native basic fibroblast growth factor N-terminal to allow it to bind to collagen.


METHODS
Sprague-Dawley rats underwent partial cystectomy. Collagen scaffolds loaded with collagen binding domain basic fibroblast growth factor, native basic fibroblast growth factor or phosphate buffered saline were grafted to the remaining host bladders, respectively. At days 30 and 90 reconstructed bladders were evaluated by histological analysis and urodynamics.


RESULTS
This targeting basic fibroblast growth factor delivery system induced satisfying bladder histological structures. It promoted more vascularization and smooth muscle cell ingrowth. Urodynamics revealed well accommodated bladder tissue with volume capacity and compliance.


CONCLUSIONS
Results show that the targeting delivery system consisting of collagen binding domain basic fibroblast growth factor and collagen membranes induced better bladder regeneration at the injury site. Thus, this targeting delivery system may be an effective strategy for bladder regeneration with potential clinical applications.
Chen
Wei
W
Department of Urology, Center of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing, People's Republic of China.
Shi
Chunying
C
Yi
Shanhong
S
Chen
Bing
B
Zhang
Weiwei
W
Fang
Zhenqiang
Z
Wei
Zhanliang
Z
Jiang
Shaoxia
S
Sun
Xianchang
X
Hou
Xianglin
X
Xiao
Zhifeng
Z
Ye
Gang
G
Dai
Jianwu
J
D000818:Animals; D003094:Collagen; D016222:Fibroblast Growth Factor 2; D006801:Humans; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D001743:Urinary Bladder / Q000502:physiology*
D016222:Fibroblast Growth Factor 2; D003094:Collagen