title: Establishment of a resource population of SLA haplotype-defined Korean native pigs.
abstract: The highly polymorphic porcine major histocompatibility complex (MHC), or the swine leukocyte antigens (SLA), has been repeatedly associated with variations in swine immune response to pathogens and vaccines as well as with production traits. The SLA antigens are also important targets for immunological recognition of foreign tissue grafts. We recently established a resource population of Korean native pigs as models for human transplantation and xenotransplantation research. In this study, 115 animals derived from three generations of the Korean native pigs were genotyped for three SLA class I (SLA-2, SLA-3 and SLA-1) and three SLA class II loci (DRB1, DQB1, DQA) using PCR with sequence-specific primers (PCR-SSP) at the allele group resolution. A total of seven SLA haplotypes (Lr-5.34, Lr-7.23, Lr-31.13, Lr-56.23, Lr-56.30, Lr-59.1, Lr-65.34), comprising six unique class I and five unique class II haplotypes, were characterized in the founding animals. Class I haplotype Lr-65.0 and class II haplotype Lr-0.34 were novel; and together with Lr-56.0 these haplotypes appeared to be breed-specific. In the progeny population, Lr-7.23 and Lr-56.30 appeared to be the most prevalent haplotypes with frequencies of 34.7% and 31.6%, respectively; the overall homozygosity was 27.4%. This resource population of SLA-defined Korean native pigs will be useful as large animal models for various transplantation and xenotransplantation experiments, as well as for dissecting the roles of SLA proteins in swine disease resistance and production traits.
authors.affiliation: Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 305-764, Korea.
authors.forename: Han-Ok
authors.initials: HO
authors.lastname: Cho
authors.forename: Chak-Sum
authors.initials: CS
authors.lastname: Ho
authors.forename: Yu-Joo
authors.initials: YJ
authors.lastname: Lee
authors.forename: In-Cheol
authors.initials: IC
authors.lastname: Cho
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Lee
authors.forename: Moon-Suck
authors.initials: MS
authors.lastname: Ko
authors.forename: Chankyu
authors.initials: C
authors.lastname: Park
authors.forename: Douglas M
authors.initials: DM
authors.lastname: Smith
authors.forename: Jin-Tae
authors.initials: JT
authors.lastname: Jeon
authors.forename: Jun-Heon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis*; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D006295:Health Resources; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D007125:Immunogenetics; D023421:Models, Animal; D016377:Organ Transplantation; D013045:Species Specificity; D013552:Swine / Q000235:genetics / Q000276:immunology
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen; D004247:DNA