title: Differential stress responses among newly received calves: variations in reductant capacity and Hsp gene expression.
abstract: Bovine respiratory disease complex (BRD), a major economic concern to the beef cattle industry all over the world, is triggered by physical, biological and psychological stresses. It is becoming noticeable that the key to reducing BRD appears to be centered at reducing the response to stress. The aims of the present study were to detect individual variations in the stress response of newly received young calves through their leukocyte heat shock protein (Hsp) response, selected neutrophil-related gene expression and oxidative stress, and relate them to pulmonary adhesions at slaughter, an indicative sign of clinical and subclinical episodes of BRD at an early age. Differential expression patterns of Hsp60 and Hsp70A1A were revealed in newly received calves 1 h, 5 h and 1 day after arrival, distinguishing between stress-responsive and non-stress-responsive individuals. Plasma cortisol was also indicative of stress-responsive and non-stress-responsive individuals, 1 h and 5 h after arrival. At the longer term, β-glycan levels were highest 7 days after arrival and significantly correlated with an adhesion-free phenotype at slaughter. Oxidative stress responses, measured through the oxidation products of the exogenous linoleoyl tyrosine (LT) marker, revealed that hydroperoxidation and epoxidation of membranes may readily occur. Based on the LT oxidation products and levels of β-glycan, we present a discriminant analysis model, according to which vulnerable individuals may be predicted at near 100% probability 7 days after arrival. Since clinical signs of BRD may often go undetected in feedlot calves, such a model, after its examination in large-scale experiments, may be a reliable tool for an early prediction of subclinical signs of BRD.
authors.affiliation: Department of Ruminant Science & Genetics, Institute of Animal Science, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, 30095, Israel.
authors.forename: Harel
authors.initials: H
authors.lastname: Eitam
authors.forename: Jacob
authors.initials: J
authors.lastname: Vaya
authors.forename: Arieh
authors.initials: A
authors.lastname: Brosh
authors.forename: Ala
authors.initials: A
authors.lastname: Orlov
authors.forename: Soliman
authors.initials: S
authors.lastname: Khatib
authors.forename: Ido
authors.initials: I
authors.lastname: Izhaki
authors.forename: Ariel
authors.initials: A
authors.lastname: Shabtay
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D048090:Bovine Respiratory Disease Complex / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D018834:Chaperonin 60 / Q000378:metabolism*; D016002:Discriminant Analysis; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006854:Hydrocortisone / Q000097:blood; D019041:L-Selectin / Q000235:genetics / Q000378:metabolism; D008041:Linoleic Acids / Q000737:chemistry / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism
chemical_list: D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D008041:Linoleic Acids; C529367:N-linoleoyltyrosine; D011509:Proteoglycans; D018125:Receptors, Transforming Growth Factor beta; D019041:L-Selectin; C071736:betaglycan; D014443:Tyrosine; D020780:Matrix Metalloproteinase 9; D006854:Hydrocortisone
references.citation: Annu Rev Genet. 1988;22:631-77
references.pmid: 2853609
references.citation: Psychosom Med. 1996 Sep-Oct;58(5):404-12
references.pmid: 8902892
references.citation: Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):770-4
references.pmid: 8421714
references.citation: J Anim Sci. 2006 Feb;84(2):488-98
references.pmid: 16424278
references.citation: J Vet Med Sci. 2004 Apr;66(4):381-7
references.pmid: 15133267
references.citation: Vet J. 2007 May;173(3):605-16
references.pmid: 16632387
references.citation: J Biol Chem. 2002 Apr 26;277(17):15028-34
references.pmid: 11836257
references.citation: J Leukoc Biol. 2004 May;75(5):815-27
references.pmid: 14761937
references.citation: Cell Stress Chaperones. 2009 Jan;14(1):61-9
references.pmid: 18548335
references.citation: Am J Vet Res. 2004 Jun;65(6):860-4
references.pmid: 15198229
references.citation: Am J Vet Res. 1999 Apr;60(4):452-7
references.pmid: 10211688
references.citation: Am J Vet Res. 1984 Oct;45(10):2003-7
references.pmid: 6437299
references.citation: J Anim Sci. 2007 Mar;85(13 Suppl):E81-8
references.pmid: 17085721
references.citation: Comp Funct Genomics. 2005;6(4):244-50
references.pmid: 18629190
references.citation: OMICS. 2007 Winter;11(4):413-34
references.pmid: 18092912
references.citation: Trends Microbiol. 2008 Feb;16(2):55-64
references.pmid: 18191570
references.citation: Cell Stress Chaperones. 2008 Dec;13(4):459-66
references.pmid: 18465207
references.citation: J Anim Sci. 1984 Sep;59(3):576-83
references.pmid: 6490547
references.citation: J Anim Sci. 2007 Aug;85(8):1885-92
references.pmid: 17504959
references.citation: Int Immunol. 2000 Nov;12(11):1539-46
references.pmid: 11058573
references.citation: Lancet. 2003 Aug 9;362(9382):469-76
references.pmid: 12927437
references.citation: Nature. 1992 Oct 22;359(6397):693-9
references.pmid: 1436033
references.citation: Annu Rev Immunol. 1998;16:137-61
references.pmid: 9597127
references.citation: J Leukoc Biol. 2008 Mar;83(3):489-92
references.pmid: 18156188
references.citation: J Anim Sci. 1999 Dec;77(12):3168-75
references.pmid: 10641860
references.citation: Nat Biotechnol. 1998 Sep;16(9):833-8
references.pmid: 9743115
references.citation: J Neuroimmunol. 2002 Dec;133(1-2):1-19
references.pmid: 12446003
references.citation: Vet Immunol Immunopathol. 2001 Dec;83(3-4):213-40
references.pmid: 11730931
references.citation: J Endocrinol. 2004 Dec;183(3):569-83
references.pmid: 15590983
references.citation: Free Radic Res. 2006 Jan;40(1):41-52
references.pmid: 16298758
references.citation: Physiol Genomics. 2006 Dec 13;28(1):97-112
references.pmid: 16912069
references.citation: J Immunol. 1999 Mar 15;162(6):3212-9
references.pmid: 10092772
references.citation: J Dairy Sci. 1993 Sep;76(9):2812-23
references.pmid: 8227685
references.citation: Br Vet J. 1987 Mar-Apr;143(2):166-74
references.pmid: 3495314
references.citation: J Leukoc Biol. 2006 Mar;79(3):425-34
references.pmid: 16387837
references.citation: Cell Cycle. 2004 Sep;3(9):1098-100
references.pmid: 15326368
references.citation: Evolution. 1989 Jan;43(1):223-225
references.pmid: 28568501
references.citation: Vet Res. 2007 Nov-Dec;38(6):809-18
references.pmid: 17727806
references.citation: Lancet Oncol. 2004 Oct;5(10):617-25
references.pmid: 15465465
references.citation: Am J Vet Res. 2000 Jan;61(1):14-9
references.pmid: 10630771
references.citation: Hum Pathol. 2006 Apr;37(4):422-30
references.pmid: 16564916
references.citation: Vet Immunol Immunopathol. 2005 May 15;105(3-4):197-219
references.pmid: 15808301
references.citation: Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):19-29
references.pmid: 17512060
references.citation: J Anim Sci. 2003 Nov;81(11):2847-55
references.pmid: 14601889
references.citation: Cell Stress Chaperones. 2003 Fall;8(3):272-86
references.pmid: 14984061
references.citation: Biomarkers. 2008 Feb;13(1):119-31
references.pmid: 17852078
references.citation: J Am Vet Med Assoc. 1996 Aug 15;209(4):814-8
references.pmid: 8756886
references.citation: FEBS Lett. 2005 Oct 24;579(25):5487-93
references.pmid: 16213498
references.citation: J Anim Sci. 1994 Jul;72(7):1891-8
references.pmid: 7928769
references.citation: Biochem J. 2004 Jul 15;381(Pt 2):413-22
references.pmid: 15086314
references.citation: Can J Comp Med. 1982 Jul;46(3):225-63
references.pmid: 6290011