title: Effect of exogenous fibrolytic enzymes supplementation on milk production and nutrient utilization in Murrah buffaloes.
abstract: The present study was conducted to evaluate the effect of commercial exogenous fibrolytic enzyme (EFE) mixture added at 1.5 and 3.0 g (cellulase 4,000 microM glucose/g/h + xylanase 7,990 microM xylose/g/min; 50:50 w/w) per kilogram of dry matter (DM) of feed on nutrient digestibility, milk production, milk composition, and some blood constituents in lactating Murrah buffaloes. Eighteen buffaloes were allotted to three dietary treatments, on the basis of milk yield (8.48, 8.52, and 8.53 kg/day) and days in lactation (68.5, 80.33, and 82.00) for 90 days. The buffaloes were fed a total mixed ration (TMR) comprising of 45% chaffed wheat straw, 15% chopped green maize, and 40% concentrate on DM basis (control group), the same TMR plus EFE at 1.5 g/kg DM (T-1 group) and the same TMR plus EFE at 3.0 g/kg DM (T-2 group) supplemented through the concentrate mixture. There was no effect of fortifying EFE mixture on DM intake and crude protein intake (grams per day) whereas total digestible nutrients intake (kilogram per day) was higher by (P < 0.05) 12.53% in T-1 group over that of control, and there was no significant difference between T-2 and control groups. The average daily milk yield and 4% fat-corrected milk (FCM) yield was higher (P < 0.05) by 12.99% and 15.17% in T-1 group as over that of control, and there was no difference between T-2 and control groups. There was no (P > 0.05) difference in blood glucose and blood urea nitrogen concentration in different experimental groups. It is concluded that supplementation of cellulase and xylanase mixture at 1.5 g/kg of DM of TMR containing wheat straw (45%), green maize (15%), and concentrate (40%) on DM basis significantly increased (P < 0.05) the average daily milk yield and FCM yield in Murrah buffaloes due to improved dietary fiber digestion.
authors.affiliation: Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal, Haryana, 132 001, India.
authors.forename: Chandra
authors.initials: C
authors.lastname: Shekhar
authors.forename: Sudarshan S
authors.initials: SS
authors.lastname: Thakur
authors.forename: Sachin K
authors.initials: SK
authors.lastname: Shelke
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena / Q000187:drug effects / Q000502:physiology; D000818:Animals; D002020:Buffaloes / Q000378:metabolism / Q000502:physiology*; D002480:Cellulase / Q000494:pharmacology*; D019587:Dietary Supplements; D004063:Digestion / Q000187:drug effects / Q000502:physiology*; D004435:Eating / Q000187:drug effects / Q000502:physiology; D043364:Endo-1,4-beta Xylanases / Q000494:pharmacology*; D005260:Female; D007774:Lactation / Q000502:physiology; D008892:Milk / Q000378:metabolism*
chemical_list: D002480:Cellulase; D043364:Endo-1,4-beta Xylanases
references.citation: J Dairy Sci. 2003 Feb;86(2):546-56
references.pmid: 12647961
references.citation: J Dairy Sci. 2000 Jun;83(6):1310-21
references.pmid: 10877396
references.citation: J Dairy Sci. 2000 Oct;83(10):2319-25
references.pmid: 11049075
references.citation: J Dairy Sci. 2000 Nov;83(11):2512-20
references.pmid: 11104270
references.citation: Anim Sci J. 2009 Jun;80(3):265-72
references.pmid: 20163634
references.citation: Br J Nutr. 2001 Mar;85(3):325-32
references.pmid: 11299078
references.citation: J Dairy Sci. 2007 Nov;90(11):5226-36
references.pmid: 17954763
references.citation: J Dairy Sci. 2002 Dec;85(12):3420-9
references.pmid: 12512615
references.citation: J Dairy Sci. 2000 Jan;83(1):115-22
references.pmid: 10659971
references.citation: J Dairy Sci. 2003 Feb;86(2):576-85
references.pmid: 12647964
references.citation: J Anim Sci. 2008 Apr;86(4):882-9
references.pmid: 18156364
references.citation: Vet Res Commun. 2007 Nov;31(8):967-75
references.pmid: 17294261