title: Overexpression of SCC-S2 correlates with lymph node metastasis and poor prognosis in patients with non-small-cell lung cancer.
abstract: The objective of the current study was to investigate the expression pattern and clinicopathological significance of SCC-S2 in patients with non-small-cell lung cancer (NSCLC). The expression profile of SCC-S2 in NSCLC tissues and adjacent noncancerous lung tissues was detected by real-time RT-PCR, western blot analysis, and immunohistochemistry. In 25 lung cancer tissues examined, 18 (72%) of them exhibited stronger levels of SCC-S2 mRNA compared with their corresponding normal tissues. SCC-S2 protein level was up-regulated in cancerous lung tissues compared to adjacent normal tissue. Moreover, the expression level of SCC-S2 in 93 archived NSCLC tissues was measured by immunohistochemical staining. SCC-S2 was found to be overexpressed in 71 of 93 (76.3%) human lung cancer samples and correlated with lymph node metastasis (P = 0.0181), p-TNM stage (P = 0.0042), Ki-67 expression (P = 0.0028), and poor survival (P = 0.012). In addition, depleting SCC-S2 expression by small-interfering RNA inhibited growth and invasion in lung cell lines. These results indicate that SCC-S2 plays an important role in NSCLC and might be a useful therapeutic target of NSCLC.
authors.affiliation: Department of Pathology, College of Basic Medical Sciences of China Medical University, Shenyang, China.
authors.forename: Qian-Ze
authors.initials: QZ
authors.lastname: Dong
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Peng-Xin
authors.initials: PX
authors.lastname: Zhang
authors.forename: Gui-Yang
authors.initials: GY
authors.lastname: Jiang
authors.forename: Xin-Jun
authors.initials: XJ
authors.lastname: Dong
authors.forename: Quan-Ze
authors.initials: QZ
authors.lastname: Cui
authors.forename: En-Hua
authors.initials: EH
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D051017:Apoptosis Regulatory Proteins; D002289:Carcinoma, Non-Small-Cell Lung / Q000401:mortality / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000401:mortality / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D051017:Apoptosis Regulatory Proteins; D011506:Proteins; D012333:RNA, Messenger; C404190:TNFAIP8 protein, human
references.citation: Lung Cancer. 2007 May;56(2):273-80
references.pmid: 17208328
references.citation: N Engl J Med. 2002 Jan 10;346(2):92-8
references.pmid: 11784875
references.citation: Clin Cancer Res. 2004 Nov 1;10(21):7335-46
references.pmid: 15534110
references.citation: Oncogene. 2004 Jan 15;23(2):612-6
references.pmid: 14724590
references.citation: Nat Med. 2003 Jun;9(6):669-76
references.pmid: 12778165
references.citation: Lung Cancer. 2005 Oct;50(1):51-8
references.pmid: 15992959
references.citation: N Engl J Med. 2007 Jan 4;356(1):11-20
references.pmid: 17202451
references.citation: J Biol Chem. 2000 Jan 28;275(4):2973-8
references.pmid: 10644768
references.citation: Annu Rev Cell Dev Biol. 2001;17:463-516
references.pmid: 11687497
references.citation: Oral Oncol. 1997 May;33(3):197-203
references.pmid: 9307729
references.citation: Mol Ther. 2006 May;13(5):947-55
references.pmid: 16455304
references.citation: Int J Cancer. 2003 Nov 20;107(4):541-50
references.pmid: 14520690
references.citation: J Clin Oncol. 2005 Dec 10;23(35):9022-8
references.pmid: 16219932
references.citation: J Biol Chem. 2001 Jul 13;276(28):26398-404
references.pmid: 11346652
references.citation: Cancer Cell. 2002 Feb;1(1):49-52
references.pmid: 12086887
references.citation: Blood. 1999 May 15;93(10):3418-31
references.pmid: 10233894
references.citation: Clin Cancer Res. 2004 Aug 15;10(16):5572-9
references.pmid: 15328199
references.citation: Curr Opin Cell Biol. 2004 Oct;16(5):558-64
references.pmid: 15363807
references.citation: Cell. 1991 Jan 25;64(2):327-36
references.pmid: 1703045
references.citation: Cancer Res. 2000 Jan 15;60(2):203-12
references.pmid: 10667560
references.citation: Nature. 1995 Jul 6;376(6535):62-6
references.pmid: 7596435
references.citation: Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9482-7
references.pmid: 12874388
references.citation: CA Cancer J Clin. 2007 Jan-Feb;57(1):43-66
references.pmid: 17237035
references.citation: Anticancer Res. 2008 Mar-Apr;28(2B):1389-97
references.pmid: 18505085