The changing fate of a secretory glycoprotein in developing maize endosperm.
Zeins are the major storage proteins in maize (Zea mays) endosperm, and their accumulation in zein bodies derived from the endoplasmic reticulum is well characterized. In contrast, relatively little is known about post-Golgi compartments or the trafficking of vacuolar proteins in maize endosperm, specifically the presence of globulins in structures resembling protein storage vacuoles that appear in early to mid-stage seed development. We investigated this pathway by expressing and analyzing a recombinant reporter glycoprotein during endosperm maturation, using a combination of microscopy and sensitive glycopeptide analysis. Specific N-glycan acceptor sites on the protein were followed through the stages of grain development, revealing a shift from predominantly paucimannosidic vacuolar glycoforms to predominantly trimmed glycan structures lacking fucose. This was accompanied by a change in the main subcellular localization of the protein from large protein storage vacuole-like post-Golgi organelles to the endoplasmic reticulum and zein bodies. The endogenous storage proteins corn alpha-globulin and corn legumin-1 showed a similar spatiotemporal profile both in transgenic plants expressing the reporter glycoprotein and in wild-type plants. This indicates that the shift of the intracellular trafficking route, as observed with our reporter glycoprotein, may be a common strategy in maize seed development.
Arcalis
Elsa
E
Department of Applied Genetics and Cell Biology and Department of Chemistry, University of Natural Resources and Applied Life Sciences, 1190 Vienna, Austria.
Stadlmann
Johannes
J
Marcel
Sylvain
S
Drakakaki
Georgia
G
Winter
Verena
V
Rodriguez
Julian
J
Fischer
Rainer
R
Altmann
Friedrich
F
Stoger
Eva
E
D010832:6-Phytase / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D056625:Endosperm / Q000254:growth & development* / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D006031:Glycosylation; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis; D021381:Protein Transport; D014617:Vacuoles / Q000378:metabolism; D003313:Zea mays / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D015028:Zein / Q000235:genetics / Q000378:metabolism*
D011134:Polysaccharides; D015028:Zein; D010832:6-Phytase
Plant Cell Physiol. 2005 Jan;46(1):245-9
15659439
Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:327-350
15012292
Biochem J. 1992 Jun 1;284 ( Pt 2):377-80
1376112
Plant Biotechnol J. 2008 Feb;6(2):189-201
17979949
Transfusion. 2006 Nov;46(11):1959-77
17076852
Plant Cell. 1989 Oct;1(10):1011-23
2562552
Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1430-5
17227846
J Exp Bot. 2001 Dec;52(365):2387-8
11709589
Glycoconj J. 2001 Jul;18(7):519-27
12151713
Plant Mol Biol. 1998 Sep;38(1-2):31-48
9738959
Anal Biochem. 2000 Oct 1;285(1):64-75
10998264
Traffic. 2005 Jan;6(1):45-55
15569244
Plant Physiol. 2006 Jun;141(2):578-86
16632592
Plant Physiol. 2004 Nov;136(3):3440-6
15542498
Planta. 2003 Feb;216(4):597-603
12569401
Plant Biotechnol J. 2005 Jan;3(1):115-27
17168904
Traffic. 2008 Mar;9(3):408-16
18194392
J Exp Bot. 2009;60(3):979-91
19174462
Plant Physiol. 2004 Nov;136(3):3457-66
15489278
J Exp Bot. 2000 May;51(346):839-45
10948209
Plant Mol Biol. 2005 Dec;59(6):869-80
16307363
Traffic. 2007 Oct;8(10):1452-64
17696967
Trends Plant Sci. 2005 Jul;10(7):316-23
15950520
J Exp Bot. 2003 Mar;54(384):913-22
12598562
J Cell Biol. 1992 Dec;119(5):1117-28
1447291
Trends Cell Biol. 1993 Dec;3(12):437-42
14731890
Int Arch Allergy Immunol. 2007;142(2):99-115
17033195
J Biol Chem. 2000 Jan 7;275(1):129-34
10617595
Eur J Biochem. 1993 Nov 15;218(1):1-27
8243456
Plant Cell. 2001 Oct;13(10):2297-317
11595803