Trichinella spiralis infection enhances protein kinase C phosphorylation in guinea pig alveolar macrophages.
To learn more about the signalling pathways involved in superoxide anion production in guinea pig alveolar macrophages, triggered by Trichinella spiralis infection, protein level and phosphorylation of mitogen activated protein (MAP) kinases and protein kinase C (PKC) were investigated. Infection with T. spiralis, the nematode having 'lung phase' during colonization of the host, enhances PKC phosphorylation in guinea pig alveolar macrophages. Isoenzymes beta and delta of PKC have been found significantly phosphorylated, although their location was not changed as a consequence of T. spiralis infection. Neither in macrophages from T. spiralis-infected guinea pig nor in platelet-activating factor (PAF)-stimulated macrophages from uninfected animals, participation of MAP kinases in respiratory burst activation was statistically significant. The parasite antigens seem to act through macrophage PAF receptors, transducing a signal for enhanced NADPH oxidase activity, as stimulating effect of newborn larvae homogenate on respiratory burst was abolished by specific PAF receptor antagonist CV 6209. A suppressive action of T. spiralis larvae on host alveolar macrophage innate immunological response was reflected by diminished protein level of ERK2 kinase and suppressed superoxide anion production, in spite of high level of PKC phosphorylation.
Dzik
J M
JM
Nencki Institute of Experimental Biology, Warszawa, Poland. j.dzik@nencki.gov.pl
Zieliński
Z
Z
Cieśla
J
J
Wałajtys-Rode
E
E
D000818:Animals; D006168:Guinea Pigs; D016676:Macrophages, Alveolar / Q000201:enzymology* / Q000276:immunology / Q000469:parasitology*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010766:Phosphorylation; D010980:Platelet Membrane Glycoproteins / Q000819:agonists; D011493:Protein Kinase C / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000819:agonists; D015398:Signal Transduction; D017160:Trichinella spiralis / Q000276:immunology*
D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D011493:Protein Kinase C; D020928:Mitogen-Activated Protein Kinases