title: Culture-independent analysis of lactic acid bacteria diversity associated with mezcal fermentation.
abstract: Mezcal is an alcoholic beverage obtained from the distillation of fermented juices of cooked Agave spp. plant stalks (agave must), and each region in Mexico with denomination of origin uses defined Agave species to prepare mezcal with unique organoleptic characteristics. During fermentation to produce mezcal in the state of Tamaulipas, not only alcohol-producing yeasts are involved, but also a lactic acid bacterial community that has not been characterized yet. In order to address this lack of knowledge on this traditional Mexican beverage, we performed a DGGE-16S rRNA analysis of the lactic acid bacterial diversity and metabolite accumulation during the fermentation of a typical agave must that is rustically produced in San Carlos County (Tamaulipas, Mexico). The analysis of metabolite production indicated a short but important malolactic fermentation stage not previously described for mezcal. The denaturing gradient gel electrophoresis (DGGE) analysis of the 16S rRNA genes showed a distinctive lactic acid bacterial community composed mainly of Pediococcus parvulus, Lactobacillus brevis, Lactobacillus composti, Lactobacillus parabuchneri, and Lactobacillus plantarum. Some atypical genera such as Weissella and Bacillus were also found in the residual must. Our results suggest that the lactic acid bacteria could strongly be implicated in the organoleptic attributes of this traditional Mexican distilled beverage.
authors.affiliation: Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Tamaulipas, Mexico. jnarvaez@ipn.mx
authors.forename: J A
authors.initials: JA
authors.lastname: Narváez-Zapata
authors.forename: R A
authors.initials: RA
authors.lastname: Rojas-Herrera
authors.forename: I C
authors.initials: IC
authors.lastname: Rodríguez-Luna
authors.forename: C P
authors.initials: CP
authors.lastname: Larralde-Corona
mesh_terms: D027882:Agave / Q000382:microbiology*; D000434:Alcoholic Beverages / Q000382:microbiology*; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001483:Base Sequence; D016172:DNA Fingerprinting; D004275:DNA, Ribosomal / Q000032:analysis; D058645:Denaturing Gradient Gel Electrophoresis; D005285:Fermentation; D005516:Food Microbiology; D019344:Lactic Acid / Q000378:metabolism*; D056546:Lactobacillales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D052196:Levilactobacillus brevis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008800:Mexico; D010376:Pediococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D058836:Weissella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid
references.citation: Lett Appl Microbiol. 2008 Mar;46(3):402-7
references.pmid: 18266637
references.citation: Appl Environ Microbiol. 1984 Nov;48(5):1034-8
references.pmid: 16346661
references.citation: Int J Food Microbiol. 2003 Jun 15;83(2):115-31
references.pmid: 12706034
references.citation: J Agric Food Chem. 2006 Feb 22;54(4):1337-41
references.pmid: 16478257
references.citation: Appl Environ Microbiol. 2007 Aug;73(16):5261-7
references.pmid: 17586664
references.citation: J Gen Appl Microbiol. 2007 Feb;53(1):7-15
references.pmid: 17429157
references.citation: Microbiology (Reading). 2001 Apr;147(Pt 4):1007-1016
references.pmid: 11283296
references.citation: Appl Environ Microbiol. 1982 Feb;43(2):357-61
references.pmid: 16345941
references.citation: Appl Environ Microbiol. 2006 May;72(5):3793-6
references.pmid: 16672538
references.citation: Int J Syst Evol Microbiol. 2007 Apr;57(Pt 4):870-872
references.pmid: 17392222
references.citation: Mol Biotechnol. 2008 Sep;40(1):13-7
references.pmid: 18373226
references.citation: Appl Environ Microbiol. 1988 Dec;54(12):3053-6
references.pmid: 16347795
references.citation: Appl Environ Microbiol. 2004 Oct;70(10):5715-31
references.pmid: 15466506
references.citation: Recent Pat Biotechnol. 2009;3(3):185-91
references.pmid: 19747148
references.citation: Appl Environ Microbiol. 2002 Jan;68(1):297-305
references.pmid: 11772639
references.citation: J Agric Food Chem. 2009 Mar 11;57(5):1933-9
references.pmid: 19216532
references.citation: Nucleic Acids Res. 1997 Jan 1;25(1):109-11
references.pmid: 9016515
references.citation: Nucleic Acids Res. 2005 Jan 1;33(Database issue):D294-6
references.pmid: 15608200
references.citation: Appl Environ Microbiol. 2003 Nov;69(11):6801-7
references.pmid: 14602643
references.citation: J Appl Microbiol. 2005;99(2):251-8
references.pmid: 16033455
references.citation: Appl Environ Microbiol. 2003 Aug;69(8):4367-74
references.pmid: 12902217
references.citation: J Biosci Bioeng. 2005 Mar;99(3):216-21
references.pmid: 16233780
references.citation: FEMS Yeast Res. 2008 Nov;8(7):1037-52
references.pmid: 18759745
references.citation: Lett Appl Microbiol. 2008 Jun;46(6):626-30
references.pmid: 18489025
references.citation: J Agric Food Chem. 2006 May 31;54(11):3911-5
references.pmid: 16719514
references.citation: Appl Environ Microbiol. 2007 Oct;73(19):6262-9
references.pmid: 17675431