Standardized preprocessing of urine for proteome analysis.
Proteome/peptidome profiling of human urine is a promising tool for the discovery of novel disease-associated biomarkers. However, a wide range of preanalytic variables influence the results of proteome/peptidome analysis regardless of the method used. We present a validated pretreatment protocol, which allows standardization of preanalytic modalities and facilitates reproducible peptidome profiling of human urine by means of magnetic bead (MB) separation in combination with MALDI-TOF MS. Such a procedure is necessary for generating consistent and reliable data from which meaningful results may be obtained for biomarker discovery and general proteomic experiments.
Fiedler
Georg Martin
GM
Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, Medical Faculty, University Leipzig, Leipzig, Germany.
Ceglarek
Uta
U
Leichtle
Alexander
A
Thiery
Joachim
J
D053000:Analytic Sample Preparation Methods / Q000379:methods* / Q000592:standards*; D002138:Calibration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D040901:Proteomics / Q000379:methods*; D013048:Specimen Handling; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016482:Urinalysis / Q000379:methods*