ELISA for RANKL-OPG complex in mouse sera.
A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for the mouse RANKL-OPG complex was developed by utilizing a polyclonal antibody that recognizes mouse soluble RANKL as an immobilized capture component and mouse OPG IgG labeled with peroxidase. We could quantify the RANKL-OPG complex level (detection limit: 1 pmol/L). Employing this assay system, we demonstrated that the RANKL-OPG complex was constitutively present in the serum of OPG+/- mice, but not in that of OPG-/- or wild-type C57BL/6 J mice.
Mogi
Makio
M
Department of Medicinal Biochemistry, School of Pharmacy, Aichi-Gakuin University, Nagoya, Japan. makio@dpc.aichi-gakuin.ac.jp
Kondo
Ayami
A
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053244:Osteoprotegerin / Q000097:blood* / Q000235:genetics; D053245:RANK Ligand / Q000097:blood*; D015203:Reproducibility of Results
D053244:Osteoprotegerin; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse