title: Testosterone depletion by castration may protect mice from heat-induced multiple organ damage and lethality.
abstract: When the vehicle-treated, sham-operated mice underwent heat stress, the fraction survival and core temperature at +4 h of body heating were found to be 5 of 15 and 34.4 degrees C +/- 0.3 degrees C, respectively. Castration 2 weeks before the start of heat stress decreased the plasma levels of testosterone almost to zero, protected the mice from heat-induced death (fraction survival, 13/15) and reduced the hypothermia (core temperature, 37.3 degrees C). The beneficial effects of castration in ameliorating lethality and hypothermia can be significantly reduced by testosterone replacement. Heat-induced apoptosis, as indicated by terminal deoxynucleotidyl- transferase- mediatedalphaUDP-biotin nick end-labeling staining, were significantly prevented by castration. In addition, heat-induced neuronal damage, as indicated by cell shrinkage and pyknosis of nucleus, to the hypothalamus was also castration-prevented. Again, the beneficial effects of castration in reducing neuronal damage to the hypothalamus as well as apoptosis in multiple organs during heatstroke, were significantly reversed by testosterone replacement. The data indicate that testosterone depletion by castration may protect mice from heatstroke-induced multiple organ damage and lethality.
authors.affiliation: The Institute of Basic Medical Sciences, National Cheng Kung University School of Medicine, Tainan, Taiwan.
authors.forename: Chian-Yuh
authors.initials: CY
authors.lastname: Lin
authors.forename: Mao-Tsun
authors.initials: MT
authors.lastname: Lin
authors.forename: Ruei-Tang
authors.initials: RT
authors.lastname: Cheng
authors.forename: Sheng-Hsien
authors.initials: SH
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001831:Body Temperature; D048429:Cell Size; D018882:Heat Stress Disorders / Q000097:blood*; D006651:Histocytochemistry; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D007668:Kidney / Q000378:metabolism; D008297:Male; D051379:Mice; D009102:Multiple Organ Failure / Q000097:blood / Q000517:prevention & control*; D009919:Orchiectomy; D013154:Spleen / Q000378:metabolism; D018709:Statistics, Nonparametric; D013739:Testosterone / Q000097:blood* / Q000172:deficiency*
chemical_list: D013739:Testosterone
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