title: The changing fate of a secretory glycoprotein in developing maize endosperm.
abstract: Zeins are the major storage proteins in maize (Zea mays) endosperm, and their accumulation in zein bodies derived from the endoplasmic reticulum is well characterized. In contrast, relatively little is known about post-Golgi compartments or the trafficking of vacuolar proteins in maize endosperm, specifically the presence of globulins in structures resembling protein storage vacuoles that appear in early to mid-stage seed development. We investigated this pathway by expressing and analyzing a recombinant reporter glycoprotein during endosperm maturation, using a combination of microscopy and sensitive glycopeptide analysis. Specific N-glycan acceptor sites on the protein were followed through the stages of grain development, revealing a shift from predominantly paucimannosidic vacuolar glycoforms to predominantly trimmed glycan structures lacking fucose. This was accompanied by a change in the main subcellular localization of the protein from large protein storage vacuole-like post-Golgi organelles to the endoplasmic reticulum and zein bodies. The endogenous storage proteins corn alpha-globulin and corn legumin-1 showed a similar spatiotemporal profile both in transgenic plants expressing the reporter glycoprotein and in wild-type plants. This indicates that the shift of the intracellular trafficking route, as observed with our reporter glycoprotein, may be a common strategy in maize seed development.
authors.affiliation: Department of Applied Genetics and Cell Biology and Department of Chemistry, University of Natural Resources and Applied Life Sciences, 1190 Vienna, Austria.
authors.forename: Elsa
authors.initials: E
authors.lastname: Arcalis
authors.forename: Johannes
authors.initials: J
authors.lastname: Stadlmann
authors.forename: Sylvain
authors.initials: S
authors.lastname: Marcel
authors.forename: Georgia
authors.initials: G
authors.lastname: Drakakaki
authors.forename: Verena
authors.initials: V
authors.lastname: Winter
authors.forename: Julian
authors.initials: J
authors.lastname: Rodriguez
authors.forename: Rainer
authors.initials: R
authors.lastname: Fischer
authors.forename: Friedrich
authors.initials: F
authors.lastname: Altmann
authors.forename: Eva
authors.initials: E
authors.lastname: Stoger
mesh_terms: D010832:6-Phytase / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D056625:Endosperm / Q000254:growth & development* / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D006031:Glycosylation; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis; D021381:Protein Transport; D014617:Vacuoles / Q000378:metabolism; D003313:Zea mays / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D015028:Zein / Q000235:genetics / Q000378:metabolism*
chemical_list: D011134:Polysaccharides; D015028:Zein; D010832:6-Phytase
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