title: Bone marrow derivation of interstitial cells of cajal in small intestine following intestinal injury.
abstract: Interstitial cells of Cajal (ICCs) in gastrointestinal tract are specialized cells serving as pacemaker cells. The origin of ICCs is currently not fully characterized. In this work, we aimed to study whether bone marrow-derived cells (BMDCs) could contribute to the origin of ICCs in the muscular plexus of small intestine using GFP-C57BL/6 chimeric mice.Engraftment of BMDCs in the intestine was investigated for GFP expression. GFP positive bone marrow mononuclear cells reached a proportion of 95.65% +/- 3.72% at different times in chimerism. Donor-derived cells distributed widely in all the layers of the gastrointestinal tract. There were GFP positive BMDCs in the myenteric plexus, which resembled characteristics of ICCs, including myenteric location, c-Kit positive staining, and ramified morphology. Donor-derived ICCs in the myenteric plexus contributed to a percentage ranging 9.25% +/- 4.9% of all the ICCs in the myenteric plexus. In conclusion, here we described that donor-derived BMDCs might differentiate into gastrointestinal ICCs after radiation injury, which provided an alternative source for the origin of the ICCs in the muscular plexus of adult intestine. These results further identified the plasticity of BMDCs and indicated therapeutic implications of BMDCs for the gastrointestinal dysmotility caused by ICCs disorders.
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Dengqun
authors.initials: D
authors.lastname: Liu
authors.forename: Fengchao
authors.initials: F
authors.lastname: Wang
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Zou
authors.forename: Shiwu
authors.initials: S
authors.lastname: Dong
authors.forename: Junping
authors.initials: J
authors.lastname: Wang
authors.forename: Xinze
authors.initials: X
authors.lastname: Ran
authors.forename: Chunxue
authors.initials: C
authors.lastname: Li
authors.forename: Chunmeng
authors.initials: C
authors.lastname: Shi
authors.forename: Yongping
authors.initials: Y
authors.lastname: Su
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics; D007150:Immunohistochemistry; D056885:Interstitial Cells of Cajal / Q000473:pathology* / Q000528:radiation effects; D007421:Intestine, Small / Q000473:pathology / Q000528:radiation effects; D008264:Macrophages / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D014184:Transplantation, Homologous
chemical_list: D049452:Green Fluorescent Proteins
references.citation: Development. 1992 Oct;116(2):369-75
references.pmid: 1283735
references.citation: Biochem Biophys Res Commun. 2007 Oct 19;362(2):252-5
references.pmid: 17707337
references.citation: Virchows Arch. 2009 Apr;454(4):401-9
references.pmid: 19214565
references.citation: Dev Dyn. 1998 Jan;211(1):60-71
references.pmid: 9438424
references.citation: Life Sci. 2002 Apr 5;70(20):2367-76
references.pmid: 12150201
references.citation: Virchows Arch. 2006 Sep;449(3):348-57
references.pmid: 16912883
references.citation: Cell Tissue Res. 2006 Aug;325(2):211-7
references.pmid: 16596393
references.citation: FASEB J. 2004 Jun;18(9):998-1000
references.pmid: 15084516
references.citation: J Biomed Sci. 2008 Sep;15(5):585-94
references.pmid: 18763056
references.citation: Dev Biol. 1996 Nov 25;180(1):97-107
references.pmid: 8948577
references.citation: Proc Natl Acad Sci U S A. 2006 Apr 18;103(16):6321-5
references.pmid: 16606845
references.citation: J Gastroenterol. 2008;43(2):124-35
references.pmid: 18306986
references.citation: Neurogastroenterol Motil. 2004 Feb;16(1):39-51
references.pmid: 14764204
references.citation: Exp Cell Res. 2009 Dec 10;315(20):3598-610
references.pmid: 19686735
references.citation: Development. 1996 Mar;122(3):725-33
references.pmid: 8631250
references.citation: Anat Rec (Hoboken). 2009 Jul;292(7):985-93
references.pmid: 19548308
references.citation: Cell. 2001 May 4;105(3):369-77
references.pmid: 11348593
references.citation: J Physiol Pharmacol. 2005 Sep;56(3):421-32
references.pmid: 16204764
references.citation: Wound Repair Regen. 2005 Jan-Feb;13(1):109-18
references.pmid: 15659043
references.citation: Stem Cells. 2006 Apr;24(4):1095-103
references.pmid: 16282438
references.citation: J Physiol. 2003 Feb 1;546(Pt 3):751-63
references.pmid: 12563001
references.citation: Gastroenterology. 1997 Jan;112(1):144-55
references.pmid: 8978353
references.citation: Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8567-70
references.pmid: 12832617
references.citation: Regen Med. 2009 Jan;4(1):119-27
references.pmid: 19105621
references.citation: Histopathology. 2007 Jan;50(2):176-89
references.pmid: 17222246
references.citation: J Neurosci. 2000 Feb 15;20(4):1393-403
references.pmid: 10662830
references.citation: Gastroenterology. 2004 Dec;127(6):1748-59
references.pmid: 15578513
references.citation: Hum Cell. 2006 May;19(2):71-5
references.pmid: 16879559
references.citation: J Gastroenterol. 2005 Jun;40(6):591-9
references.pmid: 16007393
references.citation: Gut. 2002 Jun;50(6):752-7
references.pmid: 12010874
references.citation: J Physiol. 2003 Aug 1;550(Pt 3):829-44
references.pmid: 12844505
references.citation: J Physiol. 1999 Jan 15;514 ( Pt 2):515-31
references.pmid: 9852332
references.citation: Nature. 2003 Oct 30;425(6961):968-73
references.pmid: 14555960
references.citation: Dig Dis Sci. 2010 Aug;55(8):2182-93
references.pmid: 19856101
references.citation: FEBS Lett. 1997 May 5;407(3):313-9
references.pmid: 9175875
references.citation: J Gastroenterol. 2009;44(8):834-41
references.pmid: 19458897
references.citation: Neurogastroenterol Motil. 2008 May;20 Suppl 1:54-63
references.pmid: 18402642
references.citation: Nat Med. 2002 Sep;8(9):1011-7
references.pmid: 12195435
references.citation: Dig Dis Sci. 2005 Oct;50 Suppl 1:S34-8
references.pmid: 16184419
references.citation: Gastroenterology. 2008 Apr;134(4):1083-93
references.pmid: 18395089
references.citation: Am J Physiol Gastrointest Liver Physiol. 2003 Dec;285(6):G1111-21
references.pmid: 12893628