[Simultaneous determination of three active compounds in root barks of Dictamnus dasycarpus by RP-HPLC].
OBJECTIVE
To develop a RP-HPLC method for simultaneous determination of three active compounds, dictamnine, obacunone and fraxinellone in root bark of Dictamnus dasycarpus and supply a reference for the establishment of the quality standard of D. dasycarpus.


METHODS
A Kromasil C18 column was used with methanol-water (60:40) as the mobile phase, at the flow rate of 1 mL x min(-1). 236 nm was selected as the detected wavelength.


RESULTS
The determined three compounds were well separated with a linear range of 0.0021-0.1060, 0.0201-0.9200 and 0.0102-1.020 g x L(-1), respectively. The recoveries of them were 100.5%, 99.2% and 100.2%, respectively.


CONCLUSIONS
This method is simple, rapid and accurate, particularly suitable for the quality control of D. dasycarpus.
Yang
Xiaojuan
X
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
Liu
Yanfang
Y
Bao
Zhong
Z
Jiang
Yong
Y
Tu
Pengfei
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D036905:Dictamnus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry
D010936:Plant Extracts