Ex vivo expansion of human circulating myogenic progenitors on cluster-assembled nanostructured TiO2.
Ex vivo expansion of hematopoietic stem cells has been explored in the fields of stem cell biology, gene therapy and clinical transplantation. Recently, we demonstrated the existence of a circulating myogenic progenitor expressing the CD133 antigen. The relative inability of circulating CD133+ stem cells to reproduce themselves ex vivo imposes substantial limitations on their use for clinical applications in muscular dystrophies. Here we report that the use of cluster-assembled nanostructured titanium dioxide (ns-TiO(2)) substrates, in combination with cytokine enriched medium, enables high-level expansion of circulating CD133+ stem cells in vitro. Furthermore, we demonstrate that expanded circulating CD133+ stem cells retain their in vitro capacity to differentiate into myogenic cells. The exploitation of cluster-assembled ns-TiO(2) substrates for the expansion of CD133+ stem cells in vitro could therefore make the clinical application of these stem cells for the treatment of muscle diseases practical.
Belicchi
Marzia
M
Stem Cell Laboratory, Department of Neurological Sciences, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, Università di Milano, Padiglione Ponti, Via F. Sforza 35, 20122 Milano, Italy.
Erratico
Silvia
S
Razini
Paola
P
Meregalli
Mirella
M
Cattaneo
Alessandra
A
Jacchetti
Emanuela
E
Farini
Andrea
A
Villa
Chiara
C
Bresolin
Nereo
N
Porretti
Laura
L
Lenardi
Cristina
C
Milani
Paolo
P
Torrente
Yvan
Y
D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006410:Hematopoiesis / Q000187:drug effects; D006801:Humans; D016130:Immunophenotyping; D051379:Mice; D018625:Microscopy, Atomic Force; D032342:Muscle Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D024510:Muscle Development / Q000187:drug effects; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*
D000071916:AC133 Antigen; D015703:Antigens, CD; D016207:Cytokines; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; C009495:titanium dioxide; D014025:Titanium