Recent developments in fluorescence correlation spectroscopy for diffusion measurements in planar lipid membranes.
Fluorescence correlation spectroscopy (FCS) is a single molecule technique used mainly for determination of mobility and local concentration of molecules. This review describes the specific problems of FCS in planar systems and reviews the state of the art experimental approaches such as 2-focus, Z-scan or scanning FCS, which overcome most of the artefacts and limitations of standard FCS. We focus on diffusion measurements of lipids and proteins in planar lipid membranes and review the contributions of FCS to elucidating membrane dynamics and the factors influencing it, such as membrane composition, ionic strength, presence of membrane proteins or frictional coupling with solid support.
Macháň
Radek
R
J. Heyrovský Institute of Physical Chemistry of ASCR, v.v.i., Dolejškova 2155/3, 182 23 Prague, Czech Republic.
Hof
Martin
M
J. Heyrovský Institute of Physical Chemistry of ASCR, v.v.i., Dolejškova 2155/3, 182 23 Prague, Czech Republic.
D004058:Diffusion; D008051:Lipid Bilayers / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry*; D018613:Microscopy, Confocal; D013050:Spectrometry, Fluorescence; D053835:Unilamellar Liposomes / Q000737:chemistry
D008051:Lipid Bilayers; D008563:Membrane Lipids; D053835:Unilamellar Liposomes
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