title: Phlomis mauritanica extracts reduce the xanthine oxidase activity, scavenge the superoxide anions, and inhibit the aflatoxin B1-, sodium azide-, and 4-nitrophenyldiamine-induced mutagenicity in bacteria.
abstract: Four extracts were prepared from the leaves of Phlomis mauritanica: lyophilized infusion, total oligomer flavonoids, methanol, and ethyl acetate extracts. The antimutagenic properties of these extracts were investigated by assessing the inhibition of the mutagenic effects of direct-acting mutagens such as sodium azide and 4-nitrophenylenediamine and indirect-acting mutagens like aflatoxin B1 (AFB1) using the Ames assay. The four extracts prepared from P. mauritanica strongly inhibit the mutagenicity induced by AFB1 in both Salmonella typhimurium TA 100 and TA 98 assay systems. Lyophilized infusion and methanol extracts at the dose of 250 microg per plate reduced AFB1 mutagenicity by 93% and 91%, respectively, in S. typhymurium strain TA 100. We examined also the antioxidant effect of these extracts by the enzymatic xanthine/xanthine oxidase assay. Result indicated that total oligomer flavonoids and ethyl acetate and methanol extracts were potent inhibitors of xanthine oxidase activity. In contrast, lyophilized infusion, total oligomer flavonoids, and methanol extracts exhibited a high degree of superoxide anion scavenging. Our findings emphasize the potential of P. mauritanica extracts to prevent mutations and oxidant effects. Furthermore, the results presented here could be an additional argument to support the use of this species as a medicinal and dietary plant.
authors.affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, Monastir, Tunisia.
authors.forename: Ilef
authors.initials: I
authors.lastname: Limem
authors.forename: Ines
authors.initials: I
authors.lastname: Bouhlel
authors.forename: Meriem
authors.initials: M
authors.lastname: Bouchemi
authors.forename: Soumaya
authors.initials: S
authors.lastname: Kilani
authors.forename: Jihed
authors.initials: J
authors.lastname: Boubaker
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ben-Sghaier
authors.forename: Ines
authors.initials: I
authors.lastname: Skandrani
authors.forename: Wissem
authors.initials: W
authors.lastname: Behouri
authors.forename: Aicha
authors.initials: A
authors.lastname: Neffati
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D016604:Aflatoxin B1 / Q000494:pharmacology; D000814:Aniline Compounds / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000032:analysis / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000032:analysis / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000032:analysis* / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology*; D031345:Phlomis / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D019810:Sodium Azide / Q000494:pharmacology; D013481:Superoxides / Q000737:chemistry; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: C551672:4-nitrophenyldiamine; D000814:Aniline Compounds; D016587:Antimutagenic Agents; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D009153:Mutagens; D010936:Plant Extracts; D013481:Superoxides; D019810:Sodium Azide; D016604:Aflatoxin B1; D014969:Xanthine Oxidase