title: Effects of p.C430S polymorphism in the PPARGC1A gene on muscle fibre type composition and meat quality in Yorkshire pigs.
abstract: The peroxisome proliferator-activated receptor-gamma coactivator-1A (encoded by PPARGC1A) is involved in the formation of type I fibres. Therefore, the PPARGC1A gene can be considered as a functional candidate gene for muscle fibre type composition and meat quality in pigs. The aim of this study was to investigate the associations of the p.C430S polymorphic site in exon 8 of the PPARGC1A gene with muscle fibre characteristics and meat quality traits. The polymorphism was genotyped by PCR-RFLP using AluI restriction enzyme on a total of 152 Yorkshire pigs. Statistical analyses revealed that the p.C430S genotypes significantly affected number (P < 0.05) and area (P < 0.01) of type I muscle fibre, and were significantly associated with muscle pH (P < 0.001) and lightness(P < 0.01). On the basis of these results, we suggest that the p.C430S polymorphism can induce variation of type I fibre formation in porcine longissimus dorsi muscle and that it can be used as a meaningful molecular marker for better meat quality.
authors.affiliation: Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Sungbuk-gu, Seoul, South Korea.
authors.forename: J M
authors.initials: JM
authors.lastname: Kim
authors.forename: K T
authors.initials: KT
authors.lastname: Lee
authors.forename: K S
authors.initials: KS
authors.lastname: Lim
authors.forename: E W
authors.initials: EW
authors.lastname: Park
authors.forename: Y S
authors.initials: YS
authors.lastname: Lee
authors.forename: K C
authors.initials: KC
authors.lastname: Hong
mesh_terms: D000818:Animals; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D005838:Genotype; D008297:Male; D008460:Meat; D018657:Muscle Fibers, Slow-Twitch / Q000737:chemistry*; D011110:Polymorphism, Genetic; D034421:Sus scrofa / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D014157:Transcription Factors; C111264:peroxisome-proliferator-activated receptor-gamma coactivator-1