Differential gene expression profiling on the muscle of acetylcholinesterase knockout mice: a preliminary analysis.
Acetylcholinesterase (AChE) (EC. 3.1.1.7) is the acetylcholine-hydrolyzing enzyme that plays an essential role on cholinergic neurotransmission at the synapses of the brain and at the neuromuscular junctions. In order to gain insight into the molecular mechanisms of neuromuscular dysfunction associated with AChE deficiency, we have compared the RNA expression profiles of the muscles of AChE knockout mice with those of the wild-type siblings. Total RNA from the leg muscle of the mice of the wild-type and the AChE nullizygous mice were subjected to microarray analyses with Affymetrix GeneChip((R)) Mouse Gene 1.0 ST Array. The pair-wise comparison of gene expression levels of the 28,853 mRNA transcripts showed that 303 genes were either up- or down-regulated by more than 2.0 folds in the AChE knockout mice. The interaction study of these differentially regulated genes indicated that some of these genes are clustered in biological functions that are related to lipid metabolism and the skeletal-muscular functions.
Lin
Huang-Quan
HQ
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China.
Choi
Roy
R
Chan
Kam-Leung
KL
Ip
Denis
D
Tsim
Karl Wah-Keung
KW
Wan
David Chi-Cheong
DC
D000110:Acetylcholinesterase / Q000172:deficiency* / Q000235:genetics*; D000818:Animals; D020869:Gene Expression Profiling; D055786:Gene Knockout Techniques; D051379:Mice; D018345:Mice, Knockout; D009132:Muscles / Q000201:enzymology / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000201:enzymology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D035781:Siblings
D012333:RNA, Messenger; D000110:Acetylcholinesterase