In vitro effects of silver nanoparticles on the mitochondrial respiratory chain.
Silver has been used for years in medicine; it has known antimicrobial properties. Additionally, silver has been used in water and air filtration to eliminate microorganisms, and, more recently, as a biocide to prevent infections in burns. In contact with the human body, nanoparticles can elicit a spectrum of tissue responses such as the generation of reactive oxygen species, decreased function of mitochondria and even cell death. Mitochondries are intracellular organelles that play a crucial role in ATP production. In the present work, we evaluate the in vitro effect of silver nanoparticles (AgN) on the activities of mitochondrial respiratory chain complexes from the brain, skeletal muscle, heart, and liver of rats. Our results demonstrated that AgN (10, 25, and 50 mg l(-1)) decreases the activity of mitochondrial respiratory chain complexes I, II, III, and IV from all tissues.
Costa
Cláudio Sérgio
CS
Laboratório de Síntese de Complexos Multifuncionais, Programa de Pós-graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.
Ronconi
João Vitor Vieira
JV
Daufenbach
Juliana Felipe
JF
Gonçalves
Cinara Ludvig
CL
Rezin
Gislaine Tezza
GT
Streck
Emilio Luiz
EL
Paula
Marcos Marques da Silva
MM
D000818:Animals; D001921:Brain / Q000187:drug effects; D004579:Electron Transport / Q000187:drug effects*; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism*; D042967:Electron Transport Complex I / Q000378:metabolism; D042963:Electron Transport Complex II / Q000378:metabolism; D014450:Electron Transport Complex III / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000187:drug effects*; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000201:enzymology; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology; D008931:Mitochondria, Muscle / Q000187:drug effects* / Q000201:enzymology; D053758:Nanoparticles; D018384:Oxidative Stress / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012835:Silver Nitrate / Q000494:pharmacology*
D045222:Electron Transport Chain Complex Proteins; D017382:Reactive Oxygen Species; D012835:Silver Nitrate; D042963:Electron Transport Complex II; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I; D014450:Electron Transport Complex III
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