title: Description and DNA barcoding of three new species of Leohumicola from South Africa and the United States.
abstract: Three new species of Leohumicola (anamorphic Leotiomycetes) are described using morphological characters and phylogenetic analyses of DNA barcodes. Leohumicola levissima and L. atra were isolated from soils collected after forest fires in Crater Lake National Park, United States. Leohumicola incrustata was isolated from burned fynbos from the Cape of Good Hope Nature Reserve, South Africa. The three species exhibit characteristic Leohumicola morphology but are morphologically distinct based on conidial characters. Two DNA barcode regions, the Internal Transcribed Spacer (ITS) nuclear rDNA region and the cytochrome oxidase subunit I (Cox1) mitochondrial gene, were sequenced. Single-gene parsimony, dual-gene parsimony and dual-gene Bayesian inference phylogenetic analyses support L. levissima, L. atra, L. incrustata as distinct phylogenetic species. Both ITS and Cox1 barcodes are effective for the molecular identification of Leohumicola species.
authors.affiliation: Biodiversity Theme (Mycology & Botany), Agriculture & Agri-Food Canada, Ottawa, Ontario, Canada K1A 0C6;
authors.forename: H D T
authors.initials: HD
authors.lastname: Nguyen
authors.forename: K A
authors.initials: KA
authors.lastname: Seifert
references.citation: J Mol Evol. 1980 Dec;16(2):111-20
references.pmid: 7463489
references.citation: J Mol Evol. 1985;22(2):160-74
references.pmid: 3934395
references.citation: Proc Natl Acad Sci U S A. 2006 Jan 24;103(4):968-71
references.pmid: 16418261
references.citation: Philos Trans R Soc Lond B Biol Sci. 2005 Oct 29;360(1462):1847-57
references.pmid: 16214743
references.citation: Bioinformatics. 2003 Aug 12;19(12):1572-4
references.pmid: 12912839
references.citation: Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):3901-6
references.pmid: 17360450
references.citation: Nucleic Acids Res. 2005 Jan 20;33(2):511-8
references.pmid: 15661851