Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa.
The viral haemorrhagic septicaemia virus (VHSV) comprises 4 major genotypes and a number of subtypes with, in most cases, distinct geographical distribution. A quick and simple detection method that can discriminate the different genotypes is desirable for a quick and more efficient prevention of the spread of genotypes to new geographical areas. A monoclonal antibody (MAb) against VHSV genotype IVa was produced, with the aim of providing a simple method of discriminating this genotype from the other VHSV genotypes (I, II, III and IVb). Balb/c mice were injected with purified VHSV-JF00Ehil (genotype IVa) from diseased farmed Japanese flounder. Ten hybridoma clones secreting monoclonal antibodies (MAbs) against VHSV were established. One of these, MAb VHS-10, reacted only with genotype IVa in indirect fluorescent antibody technique (IFAT) and ELISA. Using cell cultures that were transfected with each of the viral protein genes, it was shown that the MAb VHS-10 recognizes a nonlinear genotype IVa-specific epitope on the VHSV N-protein.
Ito
T
T
Tamaki Station, Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki, Mie 519-0423, Japan. takafumi@fra.affrc.go.jp
Olesen
N J
NJ
Skall
H F
HF
Sano
M
M
Kurita
J
J
Nakajima
K
K
Iida
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D003530:Cyprinidae; D019084:Fluorescent Antibody Technique, Indirect; D005838:Genotype; D031941:Hemorrhagic Septicemia, Viral / Q000235:genetics* / Q000276:immunology*; D007074:Immunoglobulin G / Q000145:classification / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D009698:Nucleoproteins