Distribution and characterization of the Escherichia coli heat-stable enterotoxin (STa) receptor throughout the intestinal tract of newborn camels (Camelus dromedaries).
Heat-stable enterotoxin (STa) secretion from Enterotoxigenic Escherichia coli (ETEC) is crucial for the pathogenesis of diarrhea in both animal and human. The goal of this study was to investigate the distribution of the STa-specific receptors in the newborn camel's enterocytes and brush border membrane vesicles (BBMVs). Flow cytometric analysis was used to investigate the density of STa-receptors on enterocytes and BBMVs prepared from anterior jejunum, posterior jejunum, ileum, and colon. Strong density and affinity of STa-receptors was present on enterocytes and BBMVs of the ileum compared to that in the other intestinal segments. It was concluded that the ileum is the major target for STa action in newborn camels.
Al-Majali
Ahmad M
AM
Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, P. O. Box 3030, Irbid, 22110, Jordan. almajali@just.edu.jo
Khalifeh
Mohammad S
MS
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism* / Q000382:microbiology; D001427:Bacterial Toxins / Q000378:metabolism; D002162:Camelus / Q000378:metabolism* / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid; D020895:Enterocytes / Q000378:metabolism; D004768:Enterotoxins / Q000378:metabolism; D029968:Escherichia coli Proteins; D005434:Flow Cytometry; D041981:Gastrointestinal Tract / Q000378:metabolism*; D006162:Guanylate Cyclase / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled; D018000:Receptors, Peptide / Q000378:metabolism*; D018709:Statistics, Nonparametric
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D018000:Receptors, Peptide; C034151:heat stable toxin (E coli); D006162:Guanylate Cyclase; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled
J Ethnopharmacol. 2009 Jan 30;121(3):372-8
19063958
Vet Res Commun. 2000 Jul;24(5):327-38
10868550
Am J Vet Res. 1992 Dec;53(12):2251-8
1362045
Infect Immun. 2008 Jul;76(7):3141-9
18426880
Lab Anim Sci. 1999 Jun;49(3):254-9
10403439
J Cell Physiol. 1993 Jul;156(1):138-44
8100232
Infect Immun. 1992 Oct;60(10):4213-20
1328055
Gut. 1995 Jun;36(6):864-73
7615275
Eur J Pharmacol. 1991 Sep 17;202(2):201-11
1724966
FEMS Immunol Med Microbiol. 2007 Feb;49(1):35-40
17094787
Acta Anat (Basel). 1995;153(3):210-9
8984830
Microbiol Rev. 1996 Mar;60(1):167-215
8852900
Microbes Infect. 2010 Feb;12(2):89-98
19883790
J Immunol Methods. 1999 Jan 1;222(1-2):65-72
10022373
FEMS Immunol Med Microbiol. 2000 Jun;28(2):97-104
10799798