A new assay for nerve fiber repulsion.
In inflammatory lesions, sympathetic nerve fibers get lost soon after the start of inflammation. To be able to identify and examine the factors that are responsible for this repulsion of the sympathetic nerve fibers we established an in vitro assay. Sympathetic trunk ganglia of postnatal mice were used for the outgrowth of axons. They were plated on poly-D-lysine-coated culture slides. Axons were encouraged to grow out by the addition of nerve growth factor-7S (NGF) from murine submaxillary gland. Using live imaging it was clearly shown that SEMA3F is a nerve-repellent factor. Two different types of nerve fiber repulsion were observed. The assay turned out to be an excellent model for the investigation of axon guidance factors of sympathetic neurons (nerve fiber repulsion/nerve fiber attraction).
Fassold
Alexander
A
Laboratory of Experimental Rheumatology and Neuroendocrino-Immunology, Division of Rheumatology, Department of Internal Medicine I, University Hospital Regensburg, Germany.
Straub
Rainer H
RH
D000818:Animals; D001369:Axons / Q000502:physiology; D001681:Biological Assay; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D005728:Ganglia, Sympathetic / Q000166:cytology*; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018715:Microscopy, Video; D009412:Nerve Fibers / Q000502:physiology*; D020932:Nerve Growth Factor / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011107:Polylysine / Q000737:chemistry; D013363:Submandibular Gland / Q000737:chemistry; D013696:Temperature
D020099:Coated Materials, Biocompatible; D003470:Culture Media; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C491229:Sema3f protein, mouse; D011107:Polylysine; D020932:Nerve Growth Factor