title: In situ detection and genotyping of individual mRNA molecules.
abstract: Increasing knowledge about the heterogeneity of mRNA expression within cell populations highlights the need to study transcripts at the level of single cells. We present a method for detection and genotyping of individual transcripts based on padlock probes and in situ target-primed rolling-circle amplification. We detect a somatic point mutation, differentiate between members of a gene family and perform multiplex detection of transcripts in human and mouse cells and tissue.
authors.affiliation: Department of Genetics and Pathology, Uppsala University, Sweden.
authors.forename: Chatarina
authors.initials: C
authors.lastname: Larsson
authors.forename: Ida
authors.initials: I
authors.lastname: Grundberg
authors.forename: Ola
authors.initials: O
authors.lastname: Söderberg
authors.forename: Mats
authors.initials: M
authors.lastname: Nilsson
mesh_terms: D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D017354:Point Mutation; D012333:RNA, Messenger / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis; D014158:Transcription, Genetic
chemical_list: D000199:Actins; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human
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