Improvement of gas transfer by hemosome.
Intravascular oxidation is a respiratory assist method used to treat acute respiratory distress syndrome (ARDS). However intravascular oxidation through higher gas exchange is needed for successful clinical applications. In this study, an attempt was made to improve the gas exchange of an intravascular lung assist device by decreasing the level of damage to the blood through the microencapsulation of hemoglobin. The results showed that a hemosome 0.8 microm in diameter could be produced by microencapsulating the hemoglobin extracted from fresh bovine blood with the phospholipids extracted from egg yolk. The oxygen saturation curve of hemosome was S-shaped, which is similar to that found in normal blood, and the P50 was 24 mmHg. The oxygen saturation in the mixed solution of hemosome and blood at a 1:4 (v/v%) ratio was similar to that of normal blood. The gas exchange of the blood-hemosome mixed solution was more effective than whole blood. Therefore, the hemosome solution is expected to improve oxygen transfer.
Kim
Gi-Beum
GB
Department of Pharmacology, College of Veterinary Medicine, Korea Zoonosis Research Institute, Chonbuk National University, Jeonju, South Korea.
Hong
Chul-Un
CU
Kim
Jin-Shang
JS
Kim
Min-Ho
MH
Kang
Hyung-Sub
HS
D000818:Animals; D001802:Blood Substitutes / Q000138:chemical synthesis*; D002417:Cattle; D004339:Drug Compounding; D004867:Equipment Design; D006454:Hemoglobins / Q000737:chemistry*; D008567:Membranes, Artificial; D010084:Oxidation-Reduction; D010107:Oxygenators, Membrane; D011659:Pulmonary Gas Exchange
D001802:Blood Substitutes; D006454:Hemoglobins; D008567:Membranes, Artificial