Salt-triggered expression of the ANAC092-dependent senescence regulon in Arabidopsis thaliana.
The NAC domain transcription factor ANAC092 plays a central role in leaf senescence in Arabidopsis thaliana. We recently identified 170 genes whose expression increases upon activation of ANAC092 in a chemically (estradiol) controlled experimental set-up, 78 of which are known senescence-associated genes (SAGs). In accordance with the well-known phenomenon that salt stress promotes early leaf senescence in many plant species, we previously observed salt stress-enhanced expression of many SAGs of the ANAC092 regulon. Global expression profiling now revealed that 36 genes, representing 46% of all ANAC092 downstream SAGs, are induced by long-term (4 days) salt stress in shoots of Arabidopsis, whereas short-term stress (6 hours) only slightly affects gene expression. Expression analysis also showed that 14 of the 36 genes are induced by hydrogen peroxide (H2O2) treatment. Additionally, 15 senescence-associated NAC genes (senNACs), including ANAC092, respond to H2O2 exposure. Our data support the model that salt-triggered senescence is at least partly mediated through the ANAC092 gene regulatory network. Other senNACs most likely contribute to the coordination of this process, potentially in concert with H2O2-mediated signaling.
Balazadeh
Salma
S
University of Potsdam, Institute of Biochemistry and Biology, Potsdam-Golm, Germany.
Wu
Anhui
A
Mueller-Roeber
Bernd
B
PLoS Biol. 2009 Apr 21;7(4):e1000090
19385720
Genes Dev. 2009 Jan 1;23(1):80-92
19095804
J Exp Bot. 2003 Oct;54(391):2285-92
12947053
Science. 2009 Feb 20;323(5917):1053-7
19229035
Plant Physiol. 2006 Jun;141(2):436-45
16603662
Mol Cell Proteomics. 2005 Oct;4(10):1558-68
16009969
Plant J. 2010 Apr;62(2):250-64
20113437
Plant Physiol. 2005 Oct;139(2):847-56
16183833
Plant Cell Physiol. 2004 Jul;45(7):923-32
15295076
Trends Plant Sci. 2005 Feb;10(2):79-87
15708345
Plant J. 2006 May;46(4):601-12
16640597
Plant J. 2005 Dec;44(6):903-16
16359384
J Cell Biol. 2005 Jan 3;168(1):17-20
15631987
Trends Plant Sci. 2004 Oct;9(10):490-8
15465684
Plant Cell Environ. 2010 Apr;33(4):453-67
19712065
Cell Mol Life Sci. 2000 May;57(5):779-95
10892343
Plant Biol (Stuttg). 2008 Sep;10 Suppl 1:63-75
18721312