title: The complete genome sequence of freesia mosaic virus and its relationship to other potyviruses.
abstract: We have completed the genomic sequence of a potyvirus, freesia mosaic virus (FreMV), and compared it to those of other known potyviruses. The full-length genome sequence of FreMV consists of 9,489 nucleotides. The large protein contains 3,077 amino acids, with an AUG start codon and UAA stop codon, containing one open reading frame typical of a potyvirus polyprotein. The polyprotein of FreMV-Kr gives rise to eleven proteins (P1, HC-pro, P3, PIPO, 6K1, CI, 6K2, VPg, NIa, NIb and CP), and putative cleavage sites of each protein were identified by sequence comparison to those of other known potyviruses. Phylogenetic analysis of the polyprotein revealed that FreMV-Kr was most closely related to PeMoV and was related to BtMV, BaRMV and PeLMV, which belong to the BCMV subgroup. This is the first information on the complete genome structure of FreMV, and the sequence information clearly supports the status of FreMV as a member of a distinct species in the genus Potyvirus.
authors.affiliation: Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University, Seoul 139-774, Korea.
authors.forename: H I
authors.initials: HI
authors.lastname: Choi
authors.forename: H R
authors.initials: HR
authors.lastname: Lim
authors.forename: Y S
authors.initials: YS
authors.lastname: Song
authors.forename: M J
authors.initials: MJ
authors.lastname: Kim
authors.forename: S H
authors.initials: SH
authors.lastname: Choi
authors.forename: Y S
authors.initials: YS
authors.lastname: Song
authors.forename: S C
authors.initials: SC
authors.lastname: Bae
authors.forename: K H
authors.initials: KH
authors.lastname: Ryu
mesh_terms: D001483:Base Sequence; D016679:Genome, Viral; D027621:Iridaceae / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000821:virology; D017800:Potyvirus / Q000235:genetics*; D012367:RNA, Viral / Q000737:chemistry
chemical_list: D012367:RNA, Viral