title: Integrated self-powered microchip biosensor for endogenous biological cyanide.
abstract: In this work we developed a fully integrated biofuel cell on a microchip, which consisted of glucose dehydrogenase supported (carbon nanotubes/thionine/gold nanoparticles)(8) multilayer as the anode, and the (carbon nanotubes/polylysine/laccase)(15) multilayer as the cathode. The as-obtained biofuel cell produced open circuit potential 620 mV and power density 302 microW cm(-2), showing great potential as a small power resource of portable electronics. Most importantly, for the first time we demonstrated the feasibility of developing a self-powered biosensor based on the inhibitive effect on microchip enzyme biofuel cell. With cyanide employed as the model analyte, this method showed a linear range of 3.0 x 10(-7) to 5.0 x 10(-4) M and a detection limit with 1.0 x 10(-7) M under the optimal conditions. The detection limit was lower than the acceptable cyanide concentration in drinking water (1.9 x 10(-6) M) according to the World Health Organization (WHO). This self-powered sensor was successfully used to detect the cyanide concentration in a real sample, cassava, which is the main carbohydrate resource in South America and Africa. This presented biosensor combined with a resistor and a multimeter demonstrated the general applicability as a fast and simple detection method in the determination of endogenous biological cyanide.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied, Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P. R. China.
authors.forename: Liu
authors.initials: L
authors.lastname: Deng
authors.forename: Chaogui
authors.initials: C
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: Shaojun
authors.initials: S
authors.lastname: Guo
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dong
mesh_terms: D001674:Bioelectric Energy Sources / Q000706:statistics & numerical data; D056804:Biofuels / Q000706:statistics & numerical data; D015374:Biosensing Techniques / Q000379:methods*; D003486:Cyanides / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D004581:Electronics; D004867:Equipment Design; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism; D042845:Laccase / Q000378:metabolism; D057230:Limit of Detection; D002365:Manihot / Q000737:chemistry*
chemical_list: D056804:Biofuels; D003486:Cyanides; D042843:Glucose 1-Dehydrogenase; D042845:Laccase