title: Dual-function nanofilm coatings with diffusion control and protein resistance.
abstract: To date, limited examples of polyelectrolyte multilayers (PEMs) can be found that truly exploit the power of layer-by-layer nanoassembly to combine multiple functions into a complex multilayer. We demonstrate that PEMs can be designed as optimized coatings for implantable biosensors, exhibiting both diffusion control and protein resistance. PEM coatings comprising strong-weak and weak-weak pairs were evaluated, resulting in decreases in glucose diffusivity up to 5 orders of magnitude compared to water. Addition of poly(ethylene glycol) (PEG)-grafted terminal layers on the base diffusion-controlling multilayers substantially improved resistance to albumin adsorption relative to unmodified PEMs. For transport-controlling films comprising strong-weak polyelectrolyte pairs, the consistent diffusivity was observed even after exposure to protein-containing solutions, indicating minimal effects of biofouling. In contrast, the transport behavior of weak-weak polyelectrolyte pairs was susceptible to alteration by protein exposure, resulting in large variation in diffusivity, even when protein-resistant outer layers were employed.
authors.affiliation: Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Jaebum
authors.initials: J
authors.lastname: Park
authors.forename: Michael J
authors.initials: MJ
authors.lastname: McShane
mesh_terms: D000327:Adsorption; D000418:Albumins / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques; D004058:Diffusion; D004573:Electrolytes / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012031:Refractometry; D013499:Surface Properties; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000418:Albumins; D001672:Biocompatible Materials; D004573:Electrolytes; D011506:Proteins; D014867:Water; D005947:Glucose