title: Interaction of common sequence variants and selected risk factors in determination of HDL cholesterol levels.
abstract: OBJECTIVE
  The aim of our study was to assess the association of common sequence variants, and selected interactions, with HDL-c plasma levels.
  
  
  METHODS
  We analysed 743 individuals (340 men and 403 women) with high mean triglyceride and LDL-c levels. The association of five polymorphic sites (ABCA1 g.1051G>A, APOA1 g.-75G>A, CETP g.-629C>A, HNF1A g.102A>C, and LIPG g.584C>T), apoE isoforms and selected interactions with HDL-c levels were evaluated using linear regression models.
  
  
  RESULTS
  After adjusting for triglycerides, sex, and BMI the only genotype with a statistically significant effect on HDL-c levels (p-value=0.004) was the CETP promoter variant. Further, linear regression model with interactions included indicated possible interplay between APOA1 genotype and menopause (p-value=0.002) and ABCA1 and APOE isoforms (p-value=0.017) on HDL-c plasma concentration.
  
  
  CONCLUSIONS
  Our study indicated that not only the CETP variant but also apoE isoforms and menopause could operate as potent modulators of HDL-c concentrations.
authors.affiliation: Institute of Biology and Medical Genetics, 1st Faculty of Medicine and General Teaching Hospital, Charles University in Prague, 12800 Prague, Czech Republic. kzidk@lf1.cuni.cz
authors.forename: Hirschfeldova
authors.initials: H
authors.lastname: Katerina
authors.forename: Sedova
authors.initials: S
authors.lastname: Michaela
authors.forename: Vrablik
authors.initials: V
authors.lastname: Michal
authors.forename: Svobodova
authors.initials: S
authors.lastname: Helena
authors.forename: Zvarova
authors.initials: Z
authors.lastname: Jana
authors.forename: Hubacek
authors.initials: H
authors.lastname: Jaroslav
authors.forename: Ceska
authors.initials: C
authors.lastname: Richard
mesh_terms: D000328:Adult; D001057:Apolipoproteins E; D053480:Cholesterol Ester Transfer Proteins / Q000235:genetics*; D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008593:Menopause; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D020033:Protein Isoforms; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood
chemical_list: D001057:Apolipoproteins E; C506642:CETP protein, human; D053480:Cholesterol Ester Transfer Proteins; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D020033:Protein Isoforms; D014280:Triglycerides