title: Measurement of ATP6V1C1 expression in brush cytology samples as a diagnostic and prognostic marker in oral squamous cell carcinoma.
abstract: BACKGROUND
  Oral squamous cell carcinoma (OSCC) is the most common malignancy of the oral cavity. Brush cytology has reemerged as a molecular tool for diagnosing this cancer. ATP6V1C1, one of the main genes regulating V-ATPase activity, has been implicated in metastasis and multiple drug resistance. The aim of this study was to measure ATP6V1C1 expression levels in OSCC and to evaluate the value of this test in the diagnosis and prognosis of OSCC.
  
  
  RESULTS
  The differences in ATP6V1C1 expression between patients and controls were statistically significant (Mann-Whitney U test = 26, p < 0.001). Receiver operating characteristic (ROC) curve analysis showed an area under the curve of 0.9476, with the following diagnostic indices: sensitivity, 81.25%, specificity, 93.75%; accuracy, 87.50%; positive predictive value, 92.86%; negative predictive value, 83.33%; positive likelihood ratio, 30; and negative likelihood ratio, 0.06.
  
  
  METHODS
  Patients with OSCC and a control group of healthy individuals were studied. The clinical and demographic variables analyzed included age, sex, smoking, tumor location and tumor stage. Brush cytology samples were obtained using a cytology brush and analyzed by real-time quantitative polymerase chain reaction for ATP6V1C1 expression.
  
  
  CONCLUSIONS
  It was confirmed that ATP6V1C1 levels were significantly higher in patients with OSCC than in healthy controls, with expression increasing with higher tumor stage. ROC analysis showed that the measurement of ATP6V1C1 expression levels is a highly sensitive and specific diagnostic method.
authors.affiliation: Oral Medicine, Oral Surgery and Implantology Unit, Faculty of Medicine and Dentistry, Entrerríos s/n, Santiago de Compostela, Spain. perezsayans@gmail.com
authors.forename: Mario
authors.initials: M
authors.lastname: Pérez-Sayáns
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Reboiras-López
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Somoza-Martín
authors.forename: Francisco
authors.initials: F
authors.lastname: Barros-Angueira
authors.forename: Pilar Gayoso
authors.initials: PG
authors.lastname: Diz
authors.forename: José Manuel Gándara
authors.initials: JM
authors.lastname: Rey
authors.forename: Abel
authors.initials: A
authors.lastname: García-García
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism*; D003581:Cytodiagnosis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D011237:Predictive Value of Tests; D011379:Prognosis; D012372:ROC Curve; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C555946:ATP6V1C1 protein, human; D025262:Vacuolar Proton-Translocating ATPases