Scale up of a novel tri-substrate fermentation for enhanced production of Aspergillus niger lipase for tallow hydrolysis.
A novel tri-substrate fermentation (TSF) process was developed for the production of lipase from Aspergillus niger MTCC 2594 using agro-industrial residues, wheat bran (WB), coconut oil cake (COC) and an agro-product, wheat rawa (WR). The lipase activity was 628.7+/-13 U/g dry substrate (U/gds) at 30 degrees C and 96 h and growth studies indicated that addition of WR significantly augmented the biomass and lipase production. Scale up of lipase production at 100g and 3 kg (3 x 1 kg) tray-level batch fermentation resulted in 96% and 83.0% of enzyme activities, respectively, at 72 h. Maximum activity of 745.7+/-11U/gds was obtained, when fermented substrate was extracted in buffer containing 1% (w/v) sodium chloride and 0.5% (w/v) Triton X-100. Furthermore, the direct application of fermented substrate for tallow hydrolysis makes the process economical for industrial production of biofuel.
Edwinoliver
N G
NG
Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai 600020, India.
Thirunavukarasu
K
K
Naidu
R B
RB
Gowthaman
M K
MK
Kambe
T Nakajima
TN
Kamini
N R
NR
D001234:Aspergillus niger / Q000201:enzymology*; D018533:Biomass; D005223:Fats / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D008049:Lipase / Q000096:biosynthesis*; D013379:Substrate Specificity
D005223:Fats; C013698:tallow; D008049:Lipase