Protective effect of sesamol on the pulmonary inflammatory response and lung injury in endotoxemic rats.
We investigated the effect of sesamol on systemic lipopolysaccharide (LPS)-induced lung inflammation in rats. Sesamol decreased lung edema and injury, significantly decreased LPS-induced cell counts, protein concentration, tumor necrosis factor (TNF)-alpha, and nitrite levels in bronchoalveolar lavage fluid, and decreased the TNF-alpha, nitrite, and inducible nitric oxide synthase protein expression in lung tissue. Further, sesamol significantly inhibited LPS-induced TNF-alpha, nitrite, inducible nitric oxide synthase expression, and nuclear factor-kappaB activation levels in primary alveolar macrophages. We hypothesize that sesamol attenuates systemic LPS-induced lung inflammation by inhibiting the alveolar macrophage inflammatory response in rats.
Chu
Pei-Yi
PY
Department of Environmental and Occupational Health, National Cheng Kung University Medical College, Tainan, Taiwan.
Chien
Se-Ping
SP
Hsu
Dur-Zong
DZ
Liu
Ming-Yie
MY
D055371:Acute Lung Injury / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D052117:Benzodioxoles / Q000494:pharmacology*; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D019446:Endotoxemia / Q000473:pathology / Q000517:prevention & control*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000633:toxicity; D016676:Macrophages, Alveolar / Q000187:drug effects; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D011014:Pneumonia / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D011650:Pulmonary Alveoli / Q000473:pathology; D011654:Pulmonary Edema / Q000139:chemically induced / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D052117:Benzodioxoles; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009573:Nitrites; D010636:Phenols; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C025583:sesamol; D052247:Nitric Oxide Synthase Type II