title: RP-HPLC Estimation of Venlafaxine Hydrochloride in Tablet Dosage Forms.
abstract: A simple, specific, accurate, and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of venlafaxine hydrochloride in tablet dosage forms. A Phenomenex Gemini C-18, 5 mum column having 250 x 4.6 mm i.d. in isocratic mode, with mobile phase containing methanol: 0.05 M potassium dihydrogen orthophosphate (70:30, v/v; pH 6.2) was used. The flow rate was 1.0 ml/min and effluents were monitored at 226 nm. Carbamazepine was used as an internal standard. The retention time of venlafaxine hydrochloride and carbamazepine were 3.7 min and 5.3 min, respectively. The method was validated for specificity, linearity, accuracy, precision, limit of quantification, limit of detection, robustness and solution stability. Limit of detection and limit of quantification for estimation of venlafaxine hydrochloride were found to be 100 ng/ml and 300 ng/ml, respectively. Recoveries of venlafaxine hydrochloride in tablet formulations were found to be in the range of 99.02-101.68%. Proposed method was successfully applied for the quantitative determination of venlafaxine hydrochloride in tablet dosage forms.
authors.affiliation: Anand Pharmacy College, Opp. Town Hall, Anand - 388 001.
authors.forename: S L
authors.initials: SL
authors.lastname: Baldania
authors.forename: K K
authors.initials: KK
authors.lastname: Bhatt
authors.forename: R S
authors.initials: RS
authors.lastname: Mehta
authors.forename: D A
authors.initials: DA
authors.lastname: Shah
authors.forename: Tejal R
authors.initials: TR
authors.lastname: Gandhi
references.citation: J Pharm Biomed Anal. 2002 Jun 15;28(6):1055-9
references.pmid: 12049970
references.citation: Ther Drug Monit. 2003 Oct;25(5):565-73
references.pmid: 14508379
references.citation: J Chromatogr B Biomed Sci Appl. 1998 Feb 13;705(2):303-8
references.pmid: 9521568
references.citation: J Chromatogr B Biomed Sci Appl. 1997 Dec 5;703(1-2):195-201
references.pmid: 9448076
references.citation: J Pharm Biomed Anal. 2007 Apr 11;43(5):1854-9
references.pmid: 17300896
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Aug 25;794(1):35-47
references.pmid: 12888196
references.citation: J Chromatogr A. 2005 Dec 9;1098(1-2):19-29
references.pmid: 16314157
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Dec 27;829(1-2):75-81
references.pmid: 16249126
references.citation: Ther Drug Monit. 2003 Oct;25(5):581-7
references.pmid: 14508381
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jun 5;820(1):33-9
references.pmid: 15866490