title: The effect of excess expression of GFP in a novel heart-specific green fluorescence zebrafish regulated by nppa enhancer at early embryonic development.
abstract: In order to study the impalpable effect of GFP in homozygous heart-specific GFP-positive zebrafish during the early stage, the researchers analyzed the heart function of morphology and physiology at the first 3 days after fertilization. This zebrafish line was produced by a large-scale Tol2 transposon mediated enhancer trap screen that generated a transgenic zebrafish with a heart-specific expression of green fluorescent protein (GFP)-tagged under control of the nppa enhancer. In situ hybridization experiments showed that the nppa:GFP line faithfully recapitulated both the spatial and temporal expressions of the endogenous nppa. Green fluorescence was intensively and specifically expressed in the myocardial cells located both in the heart chambers and in the atrioventricular canal. The embryonic heart of nppa:GFP line developed normally compared with those in the wild type. There was no difference between the nappa:GFP and wild type lines with respect to heart rate, overall size, ejection volume, and fractional shortening. Thus the excess expression of GFP in this transgenic line seemed to exert no detrimental effects on zebrafish hearts during the early stages.
authors.affiliation: The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081, Changsha, Hunan, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Huang
authors.forename: Yun
authors.initials: Y
authors.lastname: Deng
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.forename: Wuzhou
authors.initials: W
authors.lastname: Yuan
authors.forename: Yongqi
authors.initials: Y
authors.lastname: Wan
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Mo
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Li
authors.forename: Zequn
authors.initials: Z
authors.lastname: Wang
authors.forename: Yuequn
authors.initials: Y
authors.lastname: Wang
authors.forename: Karen
authors.initials: K
authors.lastname: Ocorr
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Shuo
authors.initials: S
authors.lastname: Lin
authors.forename: Xiushan
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D009320:Atrial Natriuretic Factor / Q000737:chemistry* / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000737:chemistry; D006321:Heart / Q000196:embryology* / Q000502:physiology; D017403:In Situ Hybridization; D008957:Models, Genetic; D009206:Myocardium / Q000378:metabolism*; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D019076:Transgenes; D015027:Zebrafish
chemical_list: D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; D009320:Atrial Natriuretic Factor
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