Microplate spectroscopic methods for determination of the organophosphate soman.
Two microplate spectroscopic methods for determination of organophosphates, based on inhibition of acetylcholinesterase activity, have been elaborated and evaluated for determination of the chemical weapon agent soman. The principal difference between the methods is that one measures reaction substrate concentration (elaborated from Hestrin), while the other measures reaction product (elaborated from Ellman). The linear ranges of the two methods were found to be similar. Although the limit of quantification was lower for the Ellman method (110 pM), the sensitivity coefficient was in favor of the Hestrin method (1.55-fold higher). The effects of the main soman hydrolysis products were consistent for the two methods: both methylphosphonic acid and pinacolyl methylphosphonic acid did not inhibit acetylcholinesterase activity. The main components of decontaminating solutions showed differential effects: while monoethanolamine had no influence upon results obtained by either method, hydrogen peroxide interfered with the Ellman method at far lower concentrations than with the Hestrin method. In practical applications involving samples containing hydrogen peroxide, the method based on Hestrin should be regarded as much more specific for OP determination than the Ellman method.
Prokofieva
Daria Stanislavovna
DS
Research Institute of Hygiene, Occupational Pathology and Human Ecology (RIHOPHE), Saint-Petersburg, 188663, Russia.
Voitenko
Natalia Gennadievna
NG
Gustyleva
Lyudmila Konstantinovna
LK
Babakov
Vladimir Nikolaevich
VN
Savelieva
Elena Igorevna
EI
Jenkins
Richard Owen
RO
Goncharov
Nikolay Vasilievich
NV
D002619:Chemical Warfare Agents / Q000032:analysis / Q000737:chemistry*; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods*; D012999:Soman / Q000032:analysis / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*
D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D012999:Soman