Sealing of submicrometer wells by a shear-driven lipid bilayer.
A supported lipid bilayer (SLB) was formed in a microfluidic channel by vesicle fusion. The SLB, formed on a flat part of the surface, was driven by the shear forces of a bulk flow above the SLB to a part of the surface with embedded submicrometer wells. When using a bulk solution with a pH of 9.5 the advancing lipid bilayer sealed the wells, creating free-spanning membranes, whereas at a pH of 8.0 the SLB instead followed the contour of the wells.
Jönsson
Peter
P
Department of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden. jopeter@chalmers.se
Jonsson
Magnus P
MP
Höök
Fredrik
F
D008051:Lipid Bilayers / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D044085:Microfluidics / Q000379:methods*; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D033081:Shear Strength; D013499:Surface Properties
D008051:Lipid Bilayers; D046911:Macromolecular Substances