Cholinergic activation of M2 receptors leads to context-dependent modulation of feedforward inhibition in the visual thalamus.
In many brain regions, inhibition is mediated by numerous classes of specialized interneurons, but within the rodent dorsal lateral geniculate nucleus (dLGN), a single class of interneuron is present. dLGN interneurons inhibit thalamocortical (TC) neurons and regulate the activity of TC neurons evoked by retinal ganglion cells (RGCs), thereby controlling the visually evoked signals reaching the cortex. It is not known whether neuromodulation can regulate interneuron firing mode and the resulting inhibition. Here, we examine this in brain slices. We find that cholinergic modulation regulates the output mode of these interneurons and controls the resulting inhibition in a manner that is dependent on the level of afferent activity. When few RGCs are activated, acetylcholine suppresses synaptically evoked interneuron spiking, and strongly reduces disynaptic inhibition. In contrast, when many RGCs are coincidently activated, single stimuli promote the generation of a calcium spike, and stimulation with a brief train evokes prolonged plateau potentials lasting for many seconds that in turn lead to sustained inhibition. These findings indicate that cholinergic modulation regulates feedforward inhibition in a context-dependent manner.
Antal
Miklos
M
Department of Neurobiology, Harvard Medical School, Boston, Massachusetts, USA.
Acuna-Goycolea
Claudio
C
Pressler
R Todd
RT
Blitz
Dawn M
DM
Regehr
Wade G
WG
D000109:Acetylcholine / Q000378:metabolism*; D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D004558:Electric Stimulation; D004594:Electrophysiology; D005829:Geniculate Bodies / Q000166:cytology / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D007395:Interneurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009116:Muscarine / Q000494:pharmacology; D018721:Muscarinic Agonists / Q000494:pharmacology; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D043585:Receptor, Muscarinic M2 / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D013788:Thalamus / Q000502:physiology*
D018721:Muscarinic Agonists; D043585:Receptor, Muscarinic M2; D009116:Muscarine; D000109:Acetylcholine
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