[To investigate pathogen of hand, foot and mouth disease in Shenzhen in 2008].
OBJECTIVE
To investigate EV71 and CA16 pathogen of HFMD in Shenzhen in 2008, and to provide the evidence for the prevention and treatment HFMD.


METHODS
Using RT-PCR technology to detect the EV71 and CoxA16 genes of 307 samples HFMD; sequencing the purified PCR products from 14 samples. Using ClustalW2 online analysis software for sequence and phylogenetic analysis of enterovirus 71.


RESULTS
Percentage of positive EV71 from different samples is shown as follows respectively: positive EV71 from stool samples is 24.4% (75/307), from throat swab--7.8% (24/307), from peripheral blood--12.5% (1/8). Percentage of positive CoxA16 is shown as follows respectively: positive EV71 from stool samples is 13.8% (28/203), from throat swab-11.0% (20/181). Among all the 307 samples, three are positive for both EV71 and CoxA16. EV71 and CoxA16 are not detected in the samples of cerebrospinal fluid.Comparative analysis of nucleotide sequences of EV71 with those of strains BrCr and 11 deposited in GenBank demonstrated numerous disparities from 8 samples, but residue 595 from 2 samples and residue 658 from 1 sample are variable. The phylogenetic analysis based on VP1 region demonstrates that strains from 2 samples has the nearest genetic relationship with anhui strains, the farthest with BrCr and SHH02-6, SHZH02-40, SHZH03-58 strains, also strains from other 12 samples have the farthest genetic relationship with them. The genotypes A, B and C were classified as proposed by Brown et al. (1999). The EV71 from 14 samples were the member of genotype C.


CONCLUSIONS
EV71 among the pathogen of HFMD in Shenzhen in 2008 was majority. These EV71 may belong to the same genegroup with Anhui predominant strains.
Zhang
Hong-mei
HM
Shenzhen Donghu Hospital, Shenzhen 518020, China.
Li
Cheng-rong
CR
Liu
Yong-jun
YJ
Liu
Wei-long
WL
Fu
Dan
D
Xu
Liu-mei
LM
Xie
Jing-jing
JJ
Tan
Yan
Y
Wang
Hui
H
Chen
Xin-chun
XC
Zhou
Bo-ping
BP
D002681:China; D004770:Enterovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000821:virology*; D005243:Feces / Q000821:virology; D006232:Hand, Foot and Mouth Disease / Q000821:virology*; D006801:Humans; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins