Expression, purification, crystallization and preliminary X-ray diffraction analysis of rhesus macaque CD8alphaalpha homodimer.
As a T-cell co-receptor, CD8 binds to MHC class I molecules and plays a pivotal role in the activation of cytotoxic T lymphocytes. To date, structures of CD8 have been solved for two different mammals: human and mouse. The infection of rhesus macaques (Macaca mulatta) by simian immunodeficiency virus (SIV) is the best animal model for studying HIV. In this study, the rhesus macaque CD8 (rCD8) alphaalpha homodimer was obtained and rCD8alpha exodomain protein crystals were successfully obtained for further structural analysis. Diffraction data were collected to a resolution of 2.4 A. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 46.52, b = 56.28, c = 82.40 A. These data will facilitate further studies on the structural differences between these CD8 structures and the cellular immune responses of rhesus macaque.
Zong
Lili
L
Department of Obstetrics and Gynaecology, Integrated Traditional Chinese Medicine and Western Medicine Hospital, Nanfang Medical University, Guangzhou 510310, People's Republic of China. lili_zong@yahoo.cn
Chen
Yong
Y
Yan
Jinghua
J
Zhang
Jianhua
J
D000818:Animals; D016827:CD8 Antigens / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D008253:Macaca mulatta / Q000276:immunology*; D017510:Protein Folding; D055503:Protein Multimerization
D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain
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