title: CD133, Trop-2 and alpha2beta1 integrin surface receptors as markers of putative human prostate cancer stem cells.
abstract: Cancer stem cells (CSCs) play a key role in initiation and development of cancer and are attractive targets for therapy. The identification of CSC surface receptors to be used as therapeutic targets in vivo remains a difficult task. In this study, we assessed the expression pattern of three surface receptors: CD133, Trop-2 and alpha2beta1 integrin in human prostate cancer in order to identify CSC-niches. CD133 was found to be expressed in small clusters of cells localized in focal areas of benign as well as malignant lesions, suggesting that this protein is a bona fide marker of the prostate stem/progenitor compartment. Trop-2 was localized in both basal and luminal layers of benign glands and was highly expressed in malignant lesions. Moreover, isolated cells in benign and malignant areas were found to co-express both CD133 and Trop-2. alpha2beta1 integrin was expressed in the prostatic epithelium as well as in the surrounding stroma, limiting its utility as a marker of CSCs. In summary, we demonstrate that the combination of CD133 and Trop-2 is useful to mark putative CSC-containing compartments in human prostate.
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authors.lastname: Trerotola
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authors.forename: Hira Lal
authors.initials: HL
authors.lastname: Goel
authors.forename: Jing
authors.initials: J
authors.lastname: Li
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authors.initials: S
authors.lastname: Alberti
authors.forename: Mauro
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authors.lastname: Adams
authors.forename: Zhong
authors.initials: Z
authors.lastname: Jiang
authors.forename: Lucia R
authors.initials: LR
authors.lastname: Languino
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