title: Development and validation of UPLC method for quality control of Curcuma longa Linn.: Fast simultaneous quantitation of three curcuminoids.
abstract: A new reversed phase ultra performance liquid chromatography (UPLC) method was developed for the rapid quantification of three curcuminoids (curcumin (C), desmethoxycurcumin (DMC) and bisdesmethoxycurcumin (BDMC)) in Curcuma longa Linn. (C. longa) using a Waters BEH Shield RP C(18), 2.1 mm x 100 mm, 1.7 microm column. The runtime was 2 min. The influence of column temperature and mobile phase on resolution was investigated. The method was validated according to the ICH guideline for validation of analytical procedures with respect to precision, accuracy, and linearity. The limits of detection were 40.66, 49.38 and 29.28 pg for C, DMC and BDMC, respectively. Limits of quantitation for C, DMC and BDMC, were 134.18, 164.44 and 97.50 pg, respectively. Linear range was from 3.28 to 46.08 microg/ml. The mean+/-SD percent recoveries of curcuminoids were 99.47+/-1.66, 99.50+/-1.99 and 97.77+/-2.37 of C, DMC and BDMC, respectively. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency and sensitivity. The proposed method was found to be reproducible and convenient for quantitative analysis of three curcuminoids in C. longa. This work provided some references for quality control of C. longa.
authors.affiliation: China Military Institute of Chinese Materia Medica, 302 Military Hospital, Beijing 100039, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Cheng
authors.forename: Kong
authors.initials: K
authors.lastname: Weijun
authors.forename: Luo
authors.initials: L
authors.lastname: Yun
authors.forename: Wang
authors.initials: W
authors.lastname: Jiabo
authors.forename: Wang
authors.initials: W
authors.lastname: Haitao
authors.forename: Li
authors.initials: L
authors.lastname: Qingmiao
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiaohe
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000031:analogs & derivatives* / Q000032:analysis*; D004355:Drug Stability; D057230:Limit of Detection; D015394:Molecular Structure; D011786:Quality Control
chemical_list: C542281:bisdesmethoxycurcumin; C542280:desmethoxycurcumin; D003474:Curcumin