title: Single-molecule approaches to probe the structure, kinetics, and thermodynamics of nucleoprotein complexes that regulate transcription.
abstract: Single-molecule experimentation has contributed significantly to our understanding of the mechanics of nucleoprotein complexes that regulate epigenetic switches. In this minireview, we will discuss the application of the tethered-particle motion technique, magnetic tweezers, and atomic force microscopy to (i) directly visualize and thermodynamically characterize DNA loops induced by the lac, gal, and lambda repressors and (ii) understand the mechanistic role of DNA-supercoiling and DNA-bending cofactors in both prokaryotic and eukaryotic systems.
authors.affiliation: Department of Physics, Emory University, Atlanta, Georgia 30322, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Finzi
authors.forename: David D
authors.initials: DD
authors.lastname: Dunlap
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001435:Bacteriophages / Q000378:metabolism; D001671:Biochemistry / Q000379:methods*; D004247:DNA / Q000737:chemistry; D019281:Dimerization; D044127:Epigenesis, Genetic; D029968:Escherichia coli Proteins / Q000235:genetics; D007700:Kinetics; D056968:Lac Repressors / Q000235:genetics; D008280:Magnetics; D018625:Microscopy, Atomic Force / Q000379:methods; D009698:Nucleoproteins / Q000737:chemistry*; D052898:Optical Tweezers; D012097:Repressor Proteins / Q000235:genetics; D013816:Thermodynamics; D014158:Transcription, Genetic; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; C050993:Galactose repressor proteins; D056968:Lac Repressors; D009698:Nucleoproteins; D012097:Repressor Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D004247:DNA
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