title: Male accessory gland protein reduces egg laying in a simultaneous hermaphrodite.
abstract: Seminal fluid is an important part of the ejaculate of internally fertilizing animals. This fluid contains substances that nourish and activate sperm for successful fertilization. Additionally, it contains components that influence female physiology to further enhance fertilization success of the sperm donor, possibly beyond the recipient's optimum. Although evidence for such substances abounds, few studies have unraveled their identities, and focus has been exclusively on separate-sex species. We present the first detailed study into the seminal fluid composition of a hermaphrodite (Lymnaea stagnalis). Eight novel peptides and proteins were identified from the seminal-fluid-producing prostate gland and tested for effects on oviposition, hatching and consumption. The gene for the protein found to suppress egg mass production, Ovipostatin, was sequenced, thereby providing the first fully-characterized seminal fluid substance in a simultaneous hermaphrodite. Thus, seminal fluid peptides and proteins have evolved and can play a crucial role in sexual selection even when the sexes are combined.
authors.affiliation: Animal Ecology, Faculty of Earth and Life Sciences, VU University, Amsterdam, The Netherlands. joris.koene@falw.vu.nl
authors.forename: Joris M
authors.initials: JM
authors.lastname: Koene
authors.forename: Wiebe
authors.initials: W
authors.lastname: Sloot
authors.forename: Kora
authors.initials: K
authors.lastname: Montagne-Wajer
authors.forename: Scott F
authors.initials: SF
authors.lastname: Cummins
authors.forename: Bernard M
authors.initials: BM
authors.lastname: Degnan
authors.forename: John S
authors.initials: JS
authors.lastname: Smith
authors.forename: Gregg T
authors.initials: GT
authors.lastname: Nagle
authors.forename: Andries
authors.initials: A
authors.lastname: ter Maat
mesh_terms: D000818:Animals; D012734:Disorders of Sex Development; D005260:Female; D005796:Genes; D006801:Humans; D008195:Lymnaea / Q000737:chemistry / Q000502:physiology*; D008297:Male; D010058:Oviposition; D011467:Prostate; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D017422:Sequence Analysis, DNA
chemical_list: D029589:Seminal Plasma Proteins
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