title: [Effects of human tissue kallikrein 1 gene delivery on carotid artery neointima formation after balloon angioplasty in spontaneously hypertensive rats].
abstract: OBJECTIVE
  To investigate the effects of human tissue kallikrein 1(Ad-hKLK1) gene delivery on the neointima formation in carotid arteries of spontaneously hypertensive rats (SHRs).
  
  
  METHODS
  Carotid artery restenosis was induced in male SHR rats by balloon-injury. Rats were randomly assigned into 4 groups: Sham-operated (n = 6); Angioplasty (phosphate buffered solution 50 microl, n = 8); Vector virus (control virus, 1 x 10(9) IU in 50 microl, n = 8) and Ad-hKLK1(Ad-hKLK1, 1 x 10(9) IU in 50 microl, n = 8). Rats were sacrificed 4 weeks later. The wall-to-lumen area ratio and intima/media ratio in carotid artery were assessed by image analysis in HE stained sections. The mRNA bradykinin receptor (B1R and B2R) expressions were detected by RT-PCR. The protein expression of the cycle-independent kinase inhibitors p27Kip1 and p2lCip1 were determined by Western blot analysis.
  
  
  RESULTS
  Wall-to-lumen area ratio reduced 35.6% and intima/media ratio reduced 38.8%in Ad-hKLK1 treated SHRs compared to angioplasty group (all P < 0.001). The expression of p27Kip1 and p2lCip1 increased significantly in Ad-hKLK1 treated SHRs compared with angioplasty rats (all P < 0.001). The mRNA expression of B2R was significantly upregulated in angioplasty rats compared with sham-operated rats (P < 0.05) while mRNA expression of B1R was similar between the 2 groups.
  
  
  CONCLUSIONS
  hKLK1 gene delivery may effectively reduce neointimal formation via downregulating bradykinin B2R and up-regulating the expressions of p27Kip1, p2lCip1 signaling pathways in carotid arteries of SHRs after balloon injury.
authors.affiliation: Hypertension Institution Research of Fujian, First Affiliated Hospital of Fujian Medical University, Fuzhou 350001, China.
authors.forename: Hui-zhen
authors.initials: HZ
authors.lastname: Yu
authors.forename: Liang-di
authors.initials: LD
authors.lastname: Xie
authors.forename: Peng-li
authors.initials: PL
authors.lastname: Zhu
authors.forename: Chang-sheng
authors.initials: CS
authors.lastname: Xu
authors.forename: Hua-jun
authors.initials: HJ
authors.lastname: Wang
authors.forename: Ti-yuan
authors.initials: TY
authors.lastname: Li
mesh_terms: D000800:Angioplasty, Balloon / Q000009:adverse effects*; D000818:Animals; D017536:Carotid Artery, Common / Q000473:pathology*; D018014:Gene Transfer Techniques; D006801:Humans; D008297:Male; D058426:Neointima / Q000209:etiology*; D051381:Rats; D011918:Rats, Inbred SHR; D020840:Tissue Kallikreins / Q000235:genetics*
chemical_list: D020840:Tissue Kallikreins