title: The transcription factor c-Myb primes CD4+CD8+ immature thymocytes for selection into the iNKT lineage.
abstract: Type I invariant NKT cells (iNKT cells) are a subset of alphabeta T cells characterized by the expression of an invariant alpha-chain variable region 14-alpha-chain joining region 18 (V(alpha)14J(alpha)18) T cell antigen receptor (TCR) alpha-chain. The iNKT cells derive from CD4(+)CD8(+) double-positive (DP) thymocytes, and their generation requires a long half-life of DP thymocytes to allow V(alpha)14-J(alpha)18 rearrangements, expression of glycolipid-loaded CD1d on DP thymocytes, and signaling through the signaling-activation molecule SLAM-adaptor SAP pathway. Here we show that the transcription factor c-Myb has a central role in priming DP thymocytes to enter the iNKT lineage by simultaneously regulating CD1d expression, the half-life of DP cells and expression of SLAMF1, SLAMF6 and SAP.
authors.affiliation: Immunobiology and Cancer Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA.
authors.forename: Taishan
authors.initials: T
authors.lastname: Hu
authors.forename: Amie
authors.initials: A
authors.lastname: Simmons
authors.forename: Joan
authors.initials: J
authors.lastname: Yuan
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Bender
authors.forename: Jose
authors.initials: J
authors.lastname: Alberola-Ila
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D055610:Antigens, CD1d / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016026:Bone Marrow Transplantation; D015704:CD4 Antigens / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D019070:Cell Lineage / Q000235:genetics / Q000276:immunology; D002470:Cell Survival / Q000235:genetics / Q000276:immunology; D050990:GATA3 Transcription Factor / Q000235:genetics; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D055611:Natural Killer T-Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D054504:Precursor Cells, T-Lymphoid / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011828:Radiation Chimera; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D049231:Saposins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D000071176:Signaling Lymphocytic Activation Molecule Family; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D013950:Thymus Gland / Q000166:cytology; D051020:bcl-X Protein / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D055610:Antigens, CD1d; C494812:Bcl2l1 protein, mouse; D015704:CD4 Antigens; D016827:CD8 Antigens; D050990:GATA3 Transcription Factor; D020598:Proto-Oncogene Proteins c-myb; D011956:Receptors, Cell Surface; D049231:Saposins; D000071176:Signaling Lymphocytic Activation Molecule Family; C000604062:Slamf1 protein, mouse; C000604063:Slamf6 protein, mouse; D051020:bcl-X Protein; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1
references.citation: Immunity. 2007 Nov;27(5):751-62
references.pmid: 18031695
references.citation: Nat Immunol. 2008 Sep;9(9):1055-64
references.pmid: 18660811
references.citation: Int Immunol. 2008 Jul;20(7):925-35
references.pmid: 18511409
references.citation: J Immunol. 1999 Oct 15;163(8):4091-4
references.pmid: 10510341
references.citation: Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5114-9
references.pmid: 15792999
references.citation: Immunity. 2004 Apr;20(4):477-94
references.pmid: 15084276
references.citation: Curr Opin Immunol. 2008 Jun;20(3):358-68
references.pmid: 18501573
references.citation: Cell. 1991 May 17;65(4):677-89
references.pmid: 1709592
references.citation: Immunol Rev. 2004 Aug;200:224-32
references.pmid: 15242408
references.citation: J Immunol. 2005 Dec 15;175(12):7981-8
references.pmid: 16339534
references.citation: Nat Immunol. 2001 Oct;2(10):971-8
references.pmid: 11550008
references.citation: Immunity. 2005 Jun;22(6):705-16
references.pmid: 15963785
references.citation: Nat Immunol. 2004 Jul;5(7):721-9
references.pmid: 15195090
references.citation: Eur J Immunol. 2005 May;35(5):1399-407
references.pmid: 15816002
references.citation: J Immunol. 2008 Mar 1;180(5):3007-18
references.pmid: 18292523
references.citation: J Immunol. 2008 Dec 1;181(11):7778-85
references.pmid: 19017967
references.citation: Immunity. 2004 Dec;21(6):769-80
references.pmid: 15589166
references.citation: Nat Med. 2005 Mar;11(3):340-5
references.pmid: 15711562
references.citation: J Immunol. 2007 Feb 1;178(3):1618-27
references.pmid: 17237411
references.citation: J Exp Med. 2005 Mar 7;201(5):695-701
references.pmid: 15738056
references.citation: Nat Immunol. 2008 Sep;9(9):1009-11
references.pmid: 18711440
references.citation: Genes Dev. 1999 May 1;13(9):1073-8
references.pmid: 10323859
references.citation: Immunity. 2008 Sep 19;29(3):391-403
references.pmid: 18703361
references.citation: Annu Rev Immunol. 2007;25:297-336
references.pmid: 17150027
references.citation: Proc Natl Acad Sci U S A. 2009 May 26;106(21):8641-6
references.pmid: 19423665
references.citation: Nat Immunol. 2002 May;3(5):469-76
references.pmid: 11967541
references.citation: J Exp Med. 2005 Aug 15;202(4):485-92
references.pmid: 16087715
references.citation: Science. 1997 Nov 28;278(5343):1623-6
references.pmid: 9374462
references.citation: EMBO J. 2007 Aug 8;26(15):3629-40
references.pmid: 17641686
references.citation: J Exp Med. 1996 Jan 1;183(1):289-93
references.pmid: 8551234
references.citation: J Immunol. 2008 Jul 15;181(2):907-17
references.pmid: 18606642
references.citation: J Immunol Methods. 2006 May 30;312(1-2):34-9
references.pmid: 16647712
references.citation: EMBO J. 2000 Nov 15;19(22):6112-20
references.pmid: 11080157
references.citation: J Immunol. 2005 Mar 15;174(6):3153-7
references.pmid: 15749842
references.citation: Nat Immunol. 2002 Oct;3(10):966-74
references.pmid: 12244311
references.citation: Blood. 2006 Aug 1;108(3):896-903
references.pmid: 16597594
references.citation: Nat Rev Immunol. 2007 Jul;7(7):505-18
references.pmid: 17589542
references.citation: Mamm Genome. 1996 May;7(5):335-9
references.pmid: 8661718
references.citation: Semin Immunol. 2008 Aug;20(4):247-56
references.pmid: 18585056
references.citation: Immunity. 1997 Apr;6(4):459-67
references.pmid: 9133425
references.citation: J Exp Med. 2005 Mar 21;201(6):833-6
references.pmid: 15781574
references.citation: J Immunol. 1995 May 15;154(10):5195-204
references.pmid: 7730626
references.citation: J Immunol. 2006 Nov 15;177(10):6650-9
references.pmid: 17082577
references.citation: Methods Enzymol. 2005;392:199-217
references.pmid: 15644183
references.citation: Int Immunol. 1997 Sep;9(9):1375-84
references.pmid: 9310841
references.citation: J Immunol. 2003 Sep 15;171(6):2873-8
references.pmid: 12960309
references.citation: Curr Opin Immunol. 2007 Apr;19(2):186-93
references.pmid: 17303398