title: High-throughput knockout screen in Schizosaccharomyces pombe identifies a novel gene required for efficient homolog disjunction during meiosis I.
abstract: Meiosis is the process which produces haploid gametes from diploid precursor cells. This reduction of chromosome number is achieved by two successive divisions. Whereas homologs segregate during meiosis I, sister chromatids segregate during meiosis II. To identify novel proteins required for proper segregation of chromosomes during meiosis, we applied a high-throughput knockout technique to delete 87 S. pombe genes whose expression is upregulated during meiosis and analyzed the mutant phenotypes. Using this approach, we identified a new protein, Dil1, which is required to prevent meiosis I homolog non-disjunction. We show that Dil1 acts in the dynein pathway to promote oscillatory nuclear movement during meiosis.
authors.affiliation: Max F. Perutz Laboratories, Department of Chromosome Biology, University of Vienna, Vienna, Austria.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Rumpf
authors.forename: Lubos
authors.initials: L
authors.lastname: Cipak
authors.forename: Maria
authors.initials: M
authors.lastname: Novatchkova
authors.forename: Zhang
authors.initials: Z
authors.lastname: Li
authors.forename: Silvia
authors.initials: S
authors.lastname: Polakova
authors.forename: Andrej
authors.initials: A
authors.lastname: Dudas
authors.forename: Ines
authors.initials: I
authors.lastname: Kovacikova
authors.forename: Eva
authors.initials: E
authors.lastname: Miadokova
authors.forename: Gustav
authors.initials: G
authors.lastname: Ammerer
authors.forename: Juraj
authors.initials: J
authors.lastname: Gregan
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D020090:Chromosome Segregation; D015825:Chromosomes, Fungal; D004398:Dyneins / Q000378:metabolism; D055786:Gene Knockout Techniques; D008540:Meiosis; D010641:Phenotype; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D018797:Cell Cycle Proteins; C551845:Dil1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D004398:Dyneins
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