Development and validation of an immunoluminometric assay (ILMA) for fetuin-A-glycoprotein.
BACKGROUND
To identify dialysis patients with low fetuin-A levels, a sensitive immunoluminometric assay (ILMA) was developed.


METHODS
For the two-site ILMA, one monoclonal antibody was coated to polystyrene beads and one polyclonal antibody was labelled with acridinium ester.


RESULTS
The lower detection threshold was 0.013 g/L, with the normal range 0.20-0.87 g/L (arithmetic mean 0.437 +/- 0.118 g/L). Serum fetuin-A levels in the dialysis patients were significantly lower (arithmetic mean: 0.352 +/- 0.099 g/L, p < 0.0001).


CONCLUSIONS
This ILMA has been proved to be a reliable method for the determination of serum fetuin-A concentrations in dialysis patients.
Bäumer
Amelie
A
Department of Operative Dentistry and Periodontology, University Hospital, Ruprecht-Karls-University Heidelberg, Heidelberg, Germany.
Schmidt-Gayk
Heinrich
H
Aulmann
Michael
M
Kim
Ti-Sun
TS
D000911:Antibodies, Monoclonal / Q000276:immunology; D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007674:Kidney Diseases / Q000097:blood / Q000628:therapy; D057230:Limit of Detection; D008297:Male; D012015:Reference Standards; D006435:Renal Dialysis; D060749:alpha-2-HS-Glycoprotein
C556354:AHSG protein, human; D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D060749:alpha-2-HS-Glycoprotein