title: Quantitative analysis of RNA modifications.
abstract: RNA modifications impact numerous cellular processes such as pre-mRNA splicing and protein synthesis. The elucidation of the mechanisms by which these modifications impact cellular processes necessitates the ability to both detect and quantify the presence of these modifications within RNA molecules. Here, we present a detailed procedure that allows the detection and quantification of RNA base modifications. This procedure involves a number of techniques, including oligonucleotide-affinity selection, site-specific cleavage and radiolabeling, nuclease digestion, and thin layer chromatography.
authors.affiliation: Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY, USA.
authors.forename: John
authors.initials: J
authors.lastname: Karijolich
authors.forename: Athena
authors.initials: A
authors.lastname: Kantartzis
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Yu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D051379:Mice; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism; D010766:Phosphorylation; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics*; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D016914:Ribonuclease H / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
chemical_list: D009692:Nucleic Acid Heteroduplexes; D012342:RNA, Small Nuclear; C405484:U2 small nuclear RNA; D016914:Ribonuclease H; D014529:Uridine
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