title: [Comparative study of diagnostic methods for visceral leishmaniasis in dogs from Ilha Solteira, SP].
abstract: The purpose of the present work was a comparative study of diagnostic methods for Canine Visceral Leishmaniasis (CVL) using serological methods, enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody test (IFAT), histochemical (HE) and immunohistochemical (IMHC) tests using spleen, lymph node and liver canine tissues. In addition, Polymerase Chain Reaction (PCR) was done in blood and in tissues in order to compare and confirm no conclusive and negative diagnosis by the methods above. For this study, 34 dogs were divided according to clinical signs in asymptomatic, oligosymptomatic and polisymptomatic Leishmania-infected dogs euthanized by Zoonotic Disease Control Center (CCZ) from Ilha Solteira, SP, Brazil. The positivism indexes of ELISA, IMHC, IFAT and HE were 65.0, 62.0, 56.0 and 56.0%, respectively with the highest numbers of positive dogs in polisymptomatic (92.0%) followed by oligosymptomatic (57.0%) and asymptomatic dogs (12.5%). Furthermore, PCR confirmed the positive results and detected DNA in tissues from 100% of negative dogs and 89.0% suspects raising the animal positivism index up to 97.0%. In conclusion, PCR was the most sensitive and a valuable method for a definitive CVL diagnosis.
authors.affiliation: Programa de Pós-graduação em Ciência Animal, Curso de Medicina Veterinária Preventiva e Produção Animal, Universidade Estadual Paulista UNESP Campus de Araçatuba, Rua Clóvis Pestana 93, Jardim D. Amélia, CEP 16.050-680, Araçatuba-SP, Brazil. jassis_assis@yahoo.com.br
authors.forename: Juliana
authors.initials: J
authors.lastname: de Assis
authors.forename: Nina Marí Gual Pimenta
authors.initials: NM
authors.lastname: de Queiroz
authors.forename: Rita de Cássia Vieira
authors.initials: Rde C
authors.lastname: da Silveira
authors.forename: Cáris Maroni
authors.initials: CM
authors.lastname: Nunes
authors.forename: Trícia Maria Ferreira de Sousa
authors.initials: TM
authors.lastname: Oliveira
authors.forename: Antonio Carlos Faconti de Noronha
authors.initials: AC
authors.lastname: Junior
authors.forename: Maria Francisca
authors.initials: MF
authors.lastname: Neves
authors.forename: Rosangela Zacarias
authors.initials: RZ
authors.lastname: Machado
authors.forename: Wilma Aparecida Starke
authors.initials: WA
authors.lastname: Buzetti
mesh_terms: D000818:Animals; D001938:Brazil; D019411:Clinical Laboratory Techniques; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology*; D004285:Dogs; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000662:veterinary*