[Comparison of the binding characteristics of gangliosides with Newcastle disease viruses of different animal species by high performance thin layer chromatography-virus overlay protein blot assay].
OBJECTIVE
Gangliosides on the cells surface can act as the Newcastle disease virus receptors, but the differences of receptor specificities between Newcastle disease virus strains isolated from different avian species have not been determined. Accordingly, we attempted to determine the difference of binding specificity to gangliosides between two Newcastle disease virus NA-1 (goose-origin) and F48E9 (chicken-origin).


METHODS
Gangliosides were extracted from chicken embryo fibroblast cells (CEF) and goose embryo fibroblast cells (GEF). The extracted gangliosides were characterized and quantified by high performance thin-layer chromatography. We performed virus overlay assays and hemadsorption inhibition assays to assess the binding specificity of NA-1 and F48E9 to different gangliosides.


RESULTS
The results showed that ganglioside compositions of the CEF and GEF were different. In the thin-layer chromatographic binding assay, we analyzed the binding of the virus to different gangliosides, detecting that NA-1 mainly bound to GD1a, F48E9 mainly bound to GM1, GD1a, GD1b.


CONCLUSIONS
In conclusion, our results suggest that two viruses used different receptors for entry into different target cells.
Feng
Xin
X
Animal Science and Veterinary College of Jilin University, Changchun 130062, China. xinple@163.com
Song
Zhanyun
Z
Han
Wenyu
W
Ding
Zhuang
Z
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D002522:Chlorocebus aethiops; D002855:Chromatography, Thin Layer; D005347:Fibroblasts / Q000821:virology; D005732:Gangliosides / Q000737:chemistry*; D005777:Geese; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000737:chemistry* / Q000502:physiology*; D011201:Poultry Diseases / Q000821:virology*; D011485:Protein Binding; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism; D053586:Virus Internalization
D005732:Gangliosides; D014764:Viral Proteins