title: Protection against Paracoccidioides brasiliensis infection conferred by the prophylactic administration of native and recombinant ArtinM.
abstract: We determined the prophylactic effect of both the d-mannose-binding lectin ArtinM extracted from the seeds of Artocarpus integrifolia (jackfruit) and its recombinant counterpart during the course of experimental paracoccidioidomycosis induced in BALB/c mice. Four experimental protocols of prophylaxis were employed to evaluate the most protective regimen of ArtinM administration. It was demonstrated that the best effect was obtained by administration of two ArtinM doses on days 10 and 3 before the challenge with Paracoccidioides brasiliensis. By following this protocol, the lungs of mice that received native or recombinant ArtinM exhibited reduced fungal burden and granuloma incidence. In addition, the protocol augmented contents of IL-12, IFN-gamma, TNF-alpha and NO. On the other hand, the control group consisting of untreated infected mice had higher pulmonary levels of IL-4 and IL-10. In conclusion, prophylaxis with ArtinM significantly reproduces the effect of its therapeutic administration, i.e, it confers resistance to P. brasiliensis infection in mouse models by promoting IL-12 production and favours Th1-immunity.
authors.affiliation: Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Avenida Bandeirantes 3900, Ribeirão Preto, SP, Brazil.
authors.forename: Kely C
authors.initials: KC
authors.lastname: Coltri
authors.forename: Leandro L
authors.initials: LL
authors.lastname: Oliveira
authors.forename: Luciana P
authors.initials: LP
authors.lastname: Ruas
authors.forename: Patrícia E
authors.initials: PE
authors.lastname: Vendruscolo
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Goldman
authors.forename: Ademilson
authors.initials: A
authors.lastname: Panunto-Castelo
authors.forename: Maria-Cristina
authors.initials: MC
authors.lastname: Roque-Barreira
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D031622:Artocarpus / Q000737:chemistry*; D018890:Chemoprevention / Q000379:methods*; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D037102:Lectins / Q000302:isolation & purification / Q000627:therapeutic use*; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000032:analysis; D010228:Paracoccidioides / Q000187:drug effects*; D010229:Paracoccidioidomycosis / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000235:genetics / Q000627:therapeutic use; D012639:Seeds / Q000737:chemistry
chemical_list: D000935:Antifungal Agents; D016207:Cytokines; D037102:Lectins; D011994:Recombinant Proteins; D009569:Nitric Oxide