The interaction of humic substances with the human prion protein fragment 90-231 affects its protease K resistance and cell internalization.
In this paper we analyzed the determinants and the structural effects of the interaction of human prion protein fragment 90-231 (HuPrP) with humic substances, (HS) including humic (HA) and fulvic (FA) acids, natural refractory organic polyanions widely diffused in soils and waters. We show that this interaction is mainly driven by non-specific electrostatic attraction involving regions situated within alpha-helix A and beta-sheet S1 of human PrP. FA binding to HuPrP altered its ability to acquire some PrPSc-like characteristics induced by the mild thermal denaturation of the peptide (1 h at 53 degrees C). In particular, in the presence of FA, HuPrP shows a reduced amount of beta-sheet content (as demonstrated by the reduced binding of thioflavin T), an increased sensitivity to protease K and an inhibition of the entering in the fibrillogenic pathway. FA/HuPrP interaction caused the aggregation of the peptide in unstructured macrocomplexes, as demonstrated by the altered electrophoretic migration in semi-denaturing detergent-agarose gel assay. Importantly, in the presence of FA the rate of internalization of HuPrP in human neuroblastoma cells was significantly reduced as compared to that of the beta-structured peptide. Therefore, HS inhibited the acquisition of PrP(Sc)-like structural properties that, in turn, are responsible for HuPrP intracellular accumulation and lead to neuronal death. Important implications of these data are that HuPrP-HS complexes, being unable to be internalized in living cells may represent a molecular mechanism for the reduced transmission of prion transmission from HS-rich soil also in the presence of contamination from infected animals.
Corsaro
A
A
Laboratory of Pharmacology, Dept. of Oncology, Biology and Genetics, University of Genova, Genova, Italy.
Anselmi
C
C
Polano
M
M
Aceto
A
A
Florio
T
T
De Nobili
M
M
D000595:Amino Acid Sequence; D001578:Benzopyrans / Q000378:metabolism / Q000494:pharmacology; D052160:Benzothiazoles; D002460:Cell Line; D019286:Endopeptidase K / Q000378:metabolism; D006801:Humans; D006812:Humic Substances; D066298:In Vitro Techniques; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000633:toxicity; D018621:PrPC Proteins / Q000737:chemistry / Q000378:metabolism; D018620:PrPSc Proteins / Q000737:chemistry / Q000378:metabolism; D011328:Prions / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000633:toxicity; D011485:Protein Binding; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012987:Soil / Q000032:analysis; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D013844:Thiazoles / Q000378:metabolism
D001578:Benzopyrans; D052160:Benzothiazoles; D006812:Humic Substances; D010446:Peptide Fragments; D018621:PrPC Proteins; D018620:PrPSc Proteins; D011328:Prions; D011994:Recombinant Proteins; D012987:Soil; D013844:Thiazoles; C507417:prion protein (90-231); C009462:thioflavin T; D019286:Endopeptidase K; C005023:fulvic acid