title: Hybrid PET-optical imaging using targeted probes.
abstract: Fusion imaging of radionuclide-based molecular (PET) and structural data [x-ray computed tomography (CT)] has been firmly established. Here we show that optical measurements [fluorescence-mediated tomography (FMT)] show exquisite congruence to radionuclide measurements and that information can be seamlessly integrated and visualized. Using biocompatible nanoparticles as a generic platform (containing a (18)F isotope and a far red fluorochrome), we show good correlations between FMT and PET in probe concentration (r(2) > 0.99) and spatial signal distribution (r(2) > 0.85). Using a mouse model of cancer and different imaging probes to measure tumoral proteases, macrophage content and integrin expression simultaneously, we demonstrate the distinct tumoral locations of probes in multiple channels in vivo. The findings also suggest that FMT can serve as a surrogate modality for the screening and development of radionuclide-based imaging agents.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. MNahrendorf@mgh.harvard.edu
authors.forename: Matthias
authors.initials: M
authors.lastname: Nahrendorf
authors.forename: Edmund
authors.initials: E
authors.lastname: Keliher
authors.forename: Brett
authors.initials: B
authors.lastname: Marinelli
authors.forename: Peter
authors.initials: P
authors.lastname: Waterman
authors.forename: Paolo Fumene
authors.initials: PF
authors.lastname: Feruglio
authors.forename: Lioubov
authors.initials: L
authors.lastname: Fexon
authors.forename: Misha
authors.initials: M
authors.lastname: Pivovarov
authors.forename: Filip K
authors.initials: FK
authors.lastname: Swirski
authors.forename: Mikael J
authors.initials: MJ
authors.lastname: Pittet
authors.forename: Claudio
authors.initials: C
authors.lastname: Vinegoni
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles; D009369:Neoplasms / Q000175:diagnosis*; D049268:Positron-Emission Tomography / Q000379:methods*; D014057:Tomography, X-Ray Computed / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes
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