title: AepA of Pectobacterium is not involved in the regulation of extracellular plant cell wall degrading enzymes production.
abstract: Plant cell wall degrading enzymes (PCWDE) are the major virulence determinants in phytopathogenic Pectobacterium, and their production is controlled by many regulatory factors. In this study, we focus on the role of the AepA protein, which was previously described to be a global regulator of PCWDE production in Pectobacterium carotovorum (Murata et al. in Mol Plant Microbe Interact 4:239-246, 1991). Our results show that neither inactivation nor overexpression of aepA affects PCWDE production in either Pectobacterium atrosepticum SCRI1043 or Pectobacterium carotovorum subsp. carotovorum SCC3193. The previously published observation based on the overexpression of aepA could be explained by the presence of the adjacent regulatory rsmB gene in the constructs used. Our database searches indicated that AepA belongs to the YtcJ subfamily of amidohydrolases. YtcJ-like amidohydrolases are present in bacteria, archaea, plants and some fungi. Although AepA has 28% identity with the formamide deformylase NfdA in Arthrobacter pascens F164, AepA was unable to catalyze the degradation of NdfA-specific N-substituted formamides. We conclude that AepA is a putative aminohydrolase not involved in regulation of PCWDE production.
authors.affiliation: Department of Genetics, Institute of Molecular and Cell Biology, University of Tartu, 51010 Tartu, Estonia. pontu55@hot.ee
authors.forename: Viia
authors.initials: V
authors.lastname: Kõiv
authors.forename: Liis
authors.initials: L
authors.lastname: Andresen
authors.forename: Andres
authors.initials: A
authors.lastname: Mäe
mesh_terms: D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D002473:Cell Wall / Q000378:metabolism*; D003001:Cloning, Molecular; D004798:Enzymes / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D005110:Extracellular Space / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D044043:Pectobacterium / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D010944:Plants / Q000737:chemistry / Q000382:microbiology / Q000648:ultrastructure*; D011198:Solanum tuberosum / Q000382:microbiology; D014162:Transfection; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes; D037521:Virulence Factors; D000581:Amidohydrolases
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