title: Hsp60 and Hsp10 increase in colon mucosa of Crohn’s disease and ulcerative colitis.
abstract: The purpose of this work was to determine in colon mucosa of Crohn’s disease (CD) and ulcerative colitis (UC) in relapse: a) the levels of the chaperonins Hsp60 and Hsp10; b) the quantity of inflammatory cells; and c) if the levels of chaperonins parallel those of inflammation cells. Twenty cases of CD and UC and twenty normal controls (NC) were studied using immunohistochemistry, Western blotting and immunofluorescence. Immunohistochemically, Hsp60 and Hsp10 were increased in both inflammatory bowel diseases (IBD) compared to NC. These results were confirmed by Western blotting. Hsp60 and Hsp10 occurred in the cytoplasm of epithelial cells in CD and UC but not in NC. Hsp60 and Hsp10 co-localised to epithelial cells of mucosal glands but not always in connective tissue cells of lamina propria, where only Hsp60 or, less often, Hsp10 was found. Cells typical of inflammation were significantly more abundant in CD and UC than in NC. Since chaperonins are key factors in the activation of the immune system leading to inflammation, we propose that they play a central role in the pathogenesis of the two diseases, which, consequently, ought to be studied as chaperonopathies.
authors.affiliation: Dipartimento di Patologia Umana, Università degli Studi di Palermo, Palermo, Italy.
authors.forename: Vito
authors.initials: V
authors.lastname: Rodolico
authors.forename: Giovanni
authors.initials: G
authors.lastname: Tomasello
authors.forename: Monica
authors.initials: M
authors.lastname: Zerilli
authors.forename: Anna
authors.initials: A
authors.lastname: Martorana
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pitruzzella
authors.forename: Antonella Marino
authors.initials: AM
authors.lastname: Gammazza
authors.forename: Sabrina
authors.initials: S
authors.lastname: David
authors.forename: Giovanni
authors.initials: G
authors.lastname: Zummo
authors.forename: Provvidenza
authors.initials: P
authors.lastname: Damiani
authors.forename: Salvatore
authors.initials: S
authors.lastname: Accomando
authors.forename: Everly
authors.initials: E
authors.lastname: Conway de Macario
authors.forename: Alberto J L
authors.initials: AJ
authors.lastname: Macario
authors.forename: Francesco
authors.initials: F
authors.lastname: Cappello
mesh_terms: D000328:Adult; D000368:Aged; D018835:Chaperonin 10 / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000378:metabolism*; D003424:Crohn Disease / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged
chemical_list: D018835:Chaperonin 10; D018834:Chaperonin 60
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