Application of bipolar electrodialysis to E. coli fermentation for simultaneous acetate removal and pH control.
The application of bipolar electrodialysis (BPED) for the simultaneous removal of inhibitory acetate and pH control during E. coli fermentation was investigated. A two cell pair electrodialysis module, consisting of cation exchange, anion exchange and bipolar membranes with working area of 100 cm(2) each, was integrated with a standard 7 l stirred tank bioreactor. Results showed that BPED was beneficial in terms of in situ removal of inhibitory acetate and a reduction in the amount NH(4)OH used for pH control. In batch and fed-batch BPED fermentations, base additions were decreased by up to 50% in both cases compared to electrodialysis (ED) fermentations with pH controlled at 6.7 +/- 0.1. Consequently, the final biomass (34.2 g DCW l(-1)) and recombinant protein (5.5 g l(-1)) concentrations obtained were increased by up to 37 and 20%, respectively.
Wong
Michael
M
Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.
Woodley
John M
JM
Lye
Gary J
GJ
D000085:Acetates / Q000737:chemistry*; D000641:Ammonia / Q000737:chemistry / Q000633:toxicity; D019149:Bioreactors; D003470:Culture Media; D003956:Dialysis / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration
D000085:Acetates; D003470:Culture Media; D000641:Ammonia