Analysis of SRrp86-regulated alternative splicing: control of c-Jun and IκBβ activity.
Previous work led to the hypothesis that SRrp86, a related member of the SR protein superfamily, can interact with and modulate the activity of other SR proteins. Here, we sought to test this hypothesis by examining the effect of changing SRrp86 concentrations on overall alternative splicing patterns. SpliceArrays were used to examine 9,854 splicing events in wild-type cells, cells overexpressing SRrp86, and cells treated with siRNAs to knockdown SRrp86. From among the 500 splicing events exhibiting altered splicing under these conditions, the splicing of c-Jun and IκBβ were validated as being regulated by SRrp86 resulting in altered regulation of their downstream targets. In both cases, functionally distinct isoforms were generated that demonstrate the role SRrp86 plays in controlling alternative splicing.
Solis
Amanda S
AS
Department of Biological Sciences, Vanderbilt University, Nashville, TN USA.
Patton
James G
JG
D017398:Alternative Splicing; D000818:Animals; D045744:Cell Line, Tumor; D006367:HeLa Cells; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics*; D046228:Microarray Analysis; D016328:NF-kappa B / Q000378:metabolism; D009687:Nuclear Proteins; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics*; D012326:RNA Splicing; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D000068103:Serine-Arginine Splicing Factors
C083487:I kappa B beta protein; D020672:I-kappa B Proteins; D016328:NF-kappa B; D009687:Nuclear Proteins; D016755:Proto-Oncogene Proteins c-jun; D016601:RNA-Binding Proteins; C458841:SREK1 protein, human; C486754:Srek1 protein, rat; D000068103:Serine-Arginine Splicing Factors
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