Screening of IR50 x Rathu Heenati F7 RILs and identification of SSR markers linked to brown planthopper (Nilaparvata lugens Stål) resistance in rice (Oryza sativa L.).
Brown planthopper (Nilaparvata lugens Stål) is one of the major insect pests of rice. A Sri Lankan indica rice cultivar Rathu Heenati was found to be resistant to all biotypes of the brown planthopper. In the present study, a total of 268 F(7) RILs of IR50 and Rathu Heenati were phenotyped for their level of resistance against BPH by the standard seedbox screening test (SSST) in the greenhouse. A total of 53 SSR primers mapped on the chromosome 3 were used to screen the polymorphism between the parents IR50 and Rathu Heenati, out of which eleven were found to be polymorphic between IR50 and Rathu Heenati. The eleven primers that have shown polymorphism between the IR50 and Rathu Heenati parents were genotyped in a set of five resistant RILs and five susceptible RILs along with the parents for co-segregation analysis. Among the eleven primers, two primers namely RM3180 (18.22 Mb) and RM2453 (20.19 Mb) showed complete co-segregation with resistance. The identification of SSR markers linked with BPH resistant could be used for the maker assisted selection (MAS) program in rice breeding and to map the resistant genes on rice chromosomes for further gene cloning.
Kumari
Sanju
S
Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, India. sanjudeep7@gmail.com
M Sheba
Jennifer
J
Marappan
Maheshwaran
M
Ponnuswamy
Shanmugasunderam
S
Seetharaman
Suresh
S
Pothi
Nagarajan
N
Subbarayalu
Mohankumar
M
Muthurajan
Raveendran
R
Natesan
Senthil
S
D000818:Animals; D003433:Crosses, Genetic; D017343:Genes, Plant / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D005820:Genetic Testing; D005838:Genotype; D006430:Hemiptera / Q000502:physiology*; D007113:Immunity, Innate / Q000235:genetics*; D007178:Inbreeding; D018598:Minisatellite Repeats / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000276:immunology / Q000469:parasitology*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci / Q000235:genetics; D011995:Recombination, Genetic
D005819:Genetic Markers
Mol Genet Genomics. 2006 Apr;275(4):321-9
16395578
BMC Plant Biol. 2008 Oct 14;8:102
18854007
Hereditas. 2007 May;144(2):48-52
17567441
Theor Appl Genet. 2004 Dec;110(1):182-91
15549231
Mol Plant Microbe Interact. 2003 Jan;16(1):14-24
12580278
Theor Appl Genet. 2006 Jan;112(2):288-97
16240104
Theor Appl Genet. 1990 Dec;80(6):737-45
24221103
Theor Appl Genet. 2009 Nov;119(7):1237-46
19669727
Genome. 1994 Apr;37(2):217-21
18470071
Theor Appl Genet. 2002 Feb;104(2-3):248-253
12582694