False-positive Aspergillus real-time PCR assay due to a nutritional supplement in a bone marrow transplant recipient with GVH disease.
PCR screening for circulating DNA, especially when combined with antigen testing, has shown promise for the definitive diagnosis of invasive aspergillosis. False positives for Aspergillus real-time PCR assays have been described in several reports, but no sources of fungal DNA contamination could be clearly identified. We report a false-positive case for both galactomannan (GM) antigenemia and Aspergillus PCR due to nutritional supplement intake in a bone marrow transplant recipient with digestive graft-versus-host disease. Our case report also suggests that fungal DNA can pass into the serum from the intestinal tract in the same way as fungal GM. Clinicians should be aware of this possibility, so that the administration of costly, unnecessary antifungal treatments with potential adverse side-effects can be avoided.
Millon
L
L
Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Besançon, France. laurence.millon@univ-fcomte.fr
Grenouillet
F
F
Crouzet
J
J
Larosa
F
F
Loewert
S
S
Bellanger
A P
AP
Deconinck
E
E
Legrand
F
F
D000328:Adult; D001228:Aspergillosis / Q000175:diagnosis* / Q000276:immunology; D001230:Aspergillus / Q000235:genetics*; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D004271:DNA, Fungal / Q000378:metabolism; D019587:Dietary Supplements / Q000009:adverse effects / Q000382:microbiology*; D005189:False Positive Reactions; D005690:Galactose / Q000031:analogs & derivatives; D006086:Graft vs Host Disease / Q000150:complications / Q000382:microbiology*; D006801:Humans; D016867:Immunocompromised Host / Q000276:immunology; D008297:Male; D008351:Mannans / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000592:standards*
D004271:DNA, Fungal; D008351:Mannans; C012990:galactomannan; D005690:Galactose