title: Construction and immunogenicity of replication-competent adenovirus 5 host range mutant recombinants expressing HIV-1 gp160 of SF162 and TV1 strains.
abstract: An HIV Env immunogen capable of eliciting broad immunity is critical for a successful vaccine. We constructed and characterized adenovirus 5 host range mutant (Ad5hr) recombinants encoding HIV(SF162) gp160 (subtype B) and HIV(TV1) gp160 (subtype C). Immunization of mice with one or both induced cellular immunity to subtype B and C peptides by ELISpot, and antibody responses with high binding titers to HIV Env of subtypes A, B, C, and E. Notably, Ad5hr-HIV(TV1) gp160 induced better cellular immunity than Ad5hr-HIV(SF162) gp160, either alone or following co-administration. Thus, the TV1 Env recombinant alone may be sufficient for eliciting immune responses against both subtype B and C envelopes. Further studies of Ad5hr-HIV(TV1) gp160 in rhesus macaques will evaluate the suitability of this insert for a future phase I clinical trial using a replication-competent Ad4 vector.
authors.affiliation: Section on Immune Biology of Retroviral Infection, Vaccine Branch, National Cancer Institute, Bethesda, MD 20892, USA.
authors.forename: Rachmat
authors.initials: R
authors.lastname: Hidajat
authors.forename: Seraphin
authors.initials: S
authors.lastname: Kuate
authors.forename: David
authors.initials: D
authors.lastname: Venzon
authors.forename: Vaniambadi
authors.initials: V
authors.lastname: Kalyanaraman
authors.forename: Irene
authors.initials: I
authors.lastname: Kalisz
authors.forename: James
authors.initials: J
authors.lastname: Treece
authors.forename: Ying
authors.initials: Y
authors.lastname: Lian
authors.forename: Susan W
authors.initials: SW
authors.lastname: Barnett
authors.forename: Marjorie
authors.initials: M
authors.lastname: Robert-Guroff
mesh_terms: D016915:AIDS Vaccines / Q000096:biosynthesis / Q000276:immunology*; D000256:Adenoviridae / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D005260:Female; D015483:HIV Antibodies / Q000097:blood; D019302:HIV Envelope Protein gp160 / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000517:prevention & control*; D015497:HIV-1 / Q000276:immunology; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D016915:AIDS Vaccines; D015483:HIV Antibodies; D019302:HIV Envelope Protein gp160; C514797:gp160 protein, Human immunodeficiency virus 1
references.citation: Virology. 2008 Dec 20;382(2):217-25
references.pmid: 18947849
references.citation: AIDS. 2003 Jan 24;17(2):157-66
references.pmid: 12545074
references.citation: Proc Natl Acad Sci U S A. 1984 Jun;81(12):3655-9
references.pmid: 6587381
references.citation: Proc Natl Acad Sci U S A. 1985 Feb;82(3):689-93
references.pmid: 2983309
references.citation: Virology. 2008 Mar 15;372(2):273-90
references.pmid: 18061231
references.citation: J Immunol. 2003 Apr 15;170(8):4281-9
references.pmid: 12682263
references.citation: Virology. 2006 Sep 1;352(2):438-49
references.pmid: 16780913
references.citation: Biotechniques. 1993 Nov;15(5):856-62
references.pmid: 8267981
references.citation: Virology. 2001 Jan 5;279(1):1-8
references.pmid: 11145883
references.citation: Expert Rev Vaccines. 2004 Aug;3(4 Suppl):S105-17
references.pmid: 15285710
references.citation: Virology. 2007 Mar 30;360(1):218-34
references.pmid: 17097711
references.citation: Cell. 1979 Aug;17(4):957-66
references.pmid: 114304
references.citation: Vaccine. 2003 Sep 8;21(25-26):4022-35
references.pmid: 12922139
references.citation: Virology. 2006 Sep 15;353(1):83-98
references.pmid: 16814356
references.citation: J Virol. 1988 Jun;62(6):2059-66
references.pmid: 2835510
references.citation: J Virol. 1998 Jan;72(1):225-35
references.pmid: 9420219
references.citation: J Virol. 2006 Jun;80(12):6177-81
references.pmid: 16731957
references.citation: J Virol. 1997 Nov;71(11):8531-41
references.pmid: 9343211
references.citation: Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7777-81
references.pmid: 1502197
references.citation: J Virol. 1996 Jun;70(6):3724-33
references.pmid: 8648707
references.citation: J Virol. 1988 May;62(5):1606-16
references.pmid: 2833610
references.citation: Nucleic Acids Res. 1985 Feb 11;13(3):841-57
references.pmid: 3839074
references.citation: J Immunol. 2005 Feb 15;174(4):2185-9
references.pmid: 15699150
references.citation: Proc Natl Acad Sci U S A. 1989 Nov;86(21):8575-9
references.pmid: 2813413
references.citation: J Gene Med. 2004 Feb;6 Suppl 1:S172-83
references.pmid: 14978760
references.citation: J Virol. 2005 Nov;79(21):13338-49
references.pmid: 16227256
references.citation: J Virol. 2007 May;81(10):5024-35
references.pmid: 17329327
references.citation: Virology. 2008 May 10;374(2):322-37
references.pmid: 18252262
references.citation: J Virol. 2004 Mar;78(5):2212-21
references.pmid: 14963117
references.citation: J Virol. 2003 Aug;77(16):8607-20
references.pmid: 12885879
references.citation: J Virol. 1990 Sep;64(9):4390-8
references.pmid: 2384920
references.citation: Virology. 2002 Jan 5;292(1):107-13
references.pmid: 11878913
references.citation: Nat Med. 1997 Jun;3(6):651-8
references.pmid: 9176492
references.citation: J Exp Med. 1997 Feb 17;185(4):621-8
references.pmid: 9034141
references.citation: Virology. 2006 Aug 1;351(2):291-302
references.pmid: 16678874
references.citation: Curr Biol. 1996 Mar 1;6(3):315-24
references.pmid: 8805248
references.citation: AIDS. 2001 Feb;15 Suppl 1:S10-1
references.pmid: 11293384
references.citation: J Virol. 2006 Sep;80(18):9217-25
references.pmid: 16940533
references.citation: Proc Natl Acad Sci U S A. 2008 May 27;105(21):7552-7
references.pmid: 18490657
references.citation: J Virol. 1999 Dec;73(12):10489-502
references.pmid: 10559367
references.citation: J Virol. 2007 Apr;81(7):3414-27
references.pmid: 17229693
references.citation: J Virol. 1990 Jun;64(6):2669-77
references.pmid: 1692346
references.citation: JAMA. 2008 Aug 6;300(5):520-9
references.pmid: 18677024
references.citation: Expert Opin Biol Ther. 2008 Sep;8(9):1347-63
references.pmid: 18694354
references.citation: Int Rev Immunol. 2004 Jan-Apr;23(1-2):75-111
references.pmid: 14690856
references.citation: J Virol. 2005 Aug;79(16):10200-9
references.pmid: 16051813
references.citation: J Immunol. 2009 Mar 15;182(6):3718-27
references.pmid: 19265150
references.citation: J Virol. 2009 Jan;83(2):791-801
references.pmid: 18971271