Development of an indirect ELISA for the detection of duck circovirus infection in duck flocks.
Infection with duck circovirus (DuCV) is associated with growth retardation and developmental problems in farmed ducks. To detect DuCV-specific antibody in duck serum, an indirect enzyme-linked immunosorbent assay (iELISA) method was developed using the recombinant capsid protein antigen prepared by cloning the cap (Cap) gene of DuCV FJ0601 strain into pET-32a (+) vector and expressed in Escherichia coli. Using the optimized iELISA method, DuCV-specific antibodies were detected in 157 (12.96%) of 1211 samples obtained from 17 (89.47%) of 19 meat duck flocks aged from 25 to 40 days and in 89 (22.08%) of 403 samples obtained from 9 (75%) of 12 breeder flocks aged from 14 to 61 weeks. These results indicated that the iELISA method is useful for serological diagnosis of DuCV infection and epidemiological investigation.
Liu
Shao-Ning
SN
Laboratory of Taishan Scholar Immunobiology, China.
Zhang
Xing-Xiao
XX
Zou
Jin-Feng
JF
Xie
Zhi-Jing
ZJ
Zhu
Yan-Li
YL
Zhao
Qin
Q
Zhou
En-min
EM
Jiang
Shi-Jin
SJ
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D036022:Capsid Proteins / Q000276:immunology; D018173:Circoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus; D004372:Ducks / Q000821:virology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D011994:Recombinant Proteins / Q000276:immunology; D014760:Viral Fusion Proteins / Q000276:immunology
D036022:Capsid Proteins; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins