Eastern oyster (Crassostrea virginica) delta(15)N as a bioindicator of nitrogen sources: Observations and modeling.
Stable nitrogen isotopes (delta(15)N) in bioindicators are increasingly employed to identify nitrogen sources in many ecosystems and biological characteristics of the eastern oyster (Crassostrea virginica) make it an appropriate species for this purpose. To assess nitrogen isotopic fractionation associated with assimilation and baseline variations in oyster mantle, gill, and muscle tissue delta(15)N, manipulative fieldwork in Chesapeake Bay and corresponding modeling exercises were conducted. This study (1) determined that five individuals represented an optimal sample size; (2) verified that delta(15)N in oysters from two locations converged after shared deployment to a new location reflecting a change in nitrogen sources; (3) identified required exposure time and temporal integration (four months for muscle, two to three months for gill and mantle); and (4) demonstrated seasonal delta(15)N increases in seston (summer) and oysters (winter). As bioindicators, oysters can be deployed for spatial interpolation of nitrogen sources, even in areas lacking extant populations.
Fertig
B
B
Integration and Application Network, University of Maryland Center for Environmental Science, Cambridge, MD 21613, United States. bfertig@umces.edu
Carruthers
T J B
TJ
Dennison
W C
WC
Fertig
E J
EJ
Altabet
M A
MA
D000818:Animals; D049896:Crassostrea / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D005880:Gills / Q000378:metabolism; D008956:Models, Chemical; D009132:Muscles / Q000378:metabolism; D009587:Nitrogen Isotopes / Q000378:metabolism*; D019370:Observation; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D009587:Nitrogen Isotopes; D014874:Water Pollutants, Chemical
Anal Chem. 2005 Sep 1;77(17):5589-95
16131070
Naturwissenschaften. 2004 Feb;91(2):90-3
14991147
Biol Bull. 2001 Oct;201(2):294-6
11687432
J Environ Qual. 2004 Jan-Feb;33(1):124-32
14964366
Environ Sci Technol. 2006 May 1;40(9):2910-6
16719090
Environ Monit Assess. 2002 Mar;74(2):167-92
11878641
Ecol Appl. 2007 Dec;17(8):2333-46
18213973
Mar Pollut Bull. 2001 Feb;42(2):149-56
11381886
J Biol Chem. 1988 Sep 15;263(26):13231-45
3417655
Environ Sci Technol. 2004 Jun 15;38(12):3254-62
15260321
Mar Pollut Bull. 2008 May;56(5):860-8
18348892
Rapid Commun Mass Spectrom. 2005;19(18):2497-506
16106342
Oecologia. 1983 Mar;57(1-2):32-37
28310153
Ecol Appl. 2006 Aug;16(4):1405-20
16937807
Biomarkers. 2004 Jul-Oct;9(4-5):305-30
15764295
Oecologia. 2005 Aug;144(4):570-84
15891851
Anal Chem. 2007 Jul 15;79(14):5297-303
17567102