Comparisons of Vibrio fischeri, Photobacterium phosphoreum, and recombinant luminescent using Escherichia coli as BOD measurement.
To shorten the time needed to measure biochemical oxygen demand (BOD) in water samples and to provide a rapid feedback of the real condition of water quality, we tested and evaluated the validity and reliability of luminescent bacteria Vibrio fischeri, Photobacterium phosphoreum, and recombinant Escherichia coli as potential indicators of BOD in the domestic wastewaters. The results indicate that the luminescence intensities of these strains are dependent on temperature, pH, and BOD concentration. In comparison to the standard BOD(5) method, the time needed for BOD measurement can be shortened by 90, 120, and 150 min when V. fischeri, P. phosphoreum, and recombinant E. coli, respectively, are used. Recombinant E. coli can be adapted to measure BOD in domestic wastewater containing a wide range of BOD concentrations, V. fischeri is not suitable for measuring diluted wastewater, and P. phosphoreum has only a limited application in measuring concentrated wastewater. To the best of our knowledge, this is the first report in which V. fischeri, P. phosphoreum, and recombinant luminescent E. coli are compared in terms of their potential in BOD measurement systems.
Cheng
Chiu-Yu
CY
Department of Biological Science and Technology, China University of Science and Technology, Taipei, Taiwan, ROC.
Kuo
Jong-Tar
JT
Lin
Yu-Cheng
YC
Liao
Yi-Ru
YR
Chung
Ying-Chien
YC
D048908:Aliivibrio fischeri / Q000378:metabolism*; D002138:Calibration; D004926:Escherichia coli / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D010100:Oxygen / Q000378:metabolism*; D010776:Photobacterium / Q000378:metabolism*; D011995:Recombination, Genetic; D013696:Temperature
D010100:Oxygen