title: Histological lesion of spleen and inhibition of splenocyte proliferation in broilers fed on diets excess in selenium.
abstract: The purpose of this 42-day study was to investigate the effects of excess dietary selenium on immune function by determining morphological changes of spleen and cell cycle of splenocyte. Three hundred 1-day-old avian broilers were fed on a basic diet (0.2 mg/kg selenium) or the same diet amended to contain 1, 5, 10, and 15 mg/kg selenium (Se) supplied as sodium selenite (n = 60/group). Anatomically, the spleens were shrunken in volume with pallecent color. Histopathologically, lymphopenia in splenic nodules and periarterial lymphatic sheaths and congestion of the red pulp were observed in 5, 10, and 15 mg/kg Se group. By flow cytometry method, the percentage of G(0)/G(1) phase splenocytes was significantly increased, whereas the percentages of S phase and G(2)+M phase splenocytes and the proliferation index were markedly decreased in 5, 10, and 15 mg/kg Se groups when compared with those of 0.2 mg/kg group. The results confirmed that excess dietary Se as sodium selenite in the range of 5~15 mg/kg caused growth retardation of spleen by cell cycle blockage in young chickens.
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.forename: Weiming
authors.initials: W
authors.lastname: Lai
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D004032:Diet; D009929:Organ Size / Q000187:drug effects; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects* / Q000473:pathology*
chemical_list: D012643:Selenium