title: Cultivation-independent characterization of 'Candidatus Magnetobacterium bavaricum' via ultrastructural, geochemical, ecological and metagenomic methods.
abstract: 'Candidatus Magnetobacterium bavaricum' is unusual among magnetotactic bacteria (MTB) in terms of cell size (8-10 µm long, 1.5-2 µm in diameter), cell architecture, magnetotactic behaviour and its distinct phylogenetic position in the deep-branching Nitrospira phylum. In the present study, improved magnetic enrichment techniques permitted high-resolution scanning electron microscopy and energy dispersive X-ray analysis, which revealed the intracellular organization of the magnetosome chains. Sulfur globule accumulation in the cytoplasm point towards a sulfur-oxidizing metabolism of 'Candidatus M. bavaricum'. Detailed analysis of 'Candidatus M. bavaricum' microhabitats revealed more complex distribution patterns than previously reported, with cells predominantly found in low oxygen concentration. No correlation to other geochemical parameters could be observed. In addition, the analysis of a metagenomic fosmid library revealed a 34 kb genomic fragment, which contains 33 genes, among them the complete rRNA gene operon of 'Candidatus M. bavaricum' as well as a gene encoding a putative type IV RubisCO large subunit.
authors.affiliation: LMU Department Biology I, Microbiology, Grossharderner-Str. 2, 82152, Planegg-Martinsried, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Jogler
authors.forename: M
authors.initials: M
authors.lastname: Niebler
authors.forename: W
authors.initials: W
authors.lastname: Lin
authors.forename: M
authors.initials: M
authors.lastname: Kube
authors.forename: G
authors.initials: G
authors.lastname: Wanner
authors.forename: S
authors.initials: S
authors.lastname: Kolinko
authors.forename: P
authors.initials: P
authors.lastname: Stief
authors.forename: A J
authors.initials: AJ
authors.lastname: Beck
authors.forename: D
authors.initials: D
authors.lastname: De Beer
authors.forename: N
authors.initials: N
authors.lastname: Petersen
authors.forename: Y
authors.initials: Y
authors.lastname: Pan
authors.forename: R
authors.initials: R
authors.lastname: Amann
authors.forename: R
authors.initials: R
authors.lastname: Reinhardt
authors.forename: D
authors.initials: D
authors.lastname: Schüler
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000235:genetics; D004463:Ecology; D019015:Geologic Sediments / Q000382:microbiology; D056186:Metagenomics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012273:Ribulose-Bisphosphate Carboxylase