title: Development of transgenic rice containing a mutated beta subunit of soybean beta-conglycinin for enhanced phagocytosis-stimulating activity.
abstract: Improving the nutraceutical value of rice would positively impact the health and well-being of rice consumers worldwide. Based on the three-dimensional structure of soybean beta-conglycinin, we designed a beta subunit with a strong phagocytosis-stimulating activity (mbeta subunit). Here, we describe the genetic modification and production of rice seeds containing the mbeta subunit as part of our aim to develop a food material that promotes human health. The mbeta subunit folded correctly and was accumulated in the protein body II of rice seeds at a level similar to wild-type beta subunit. Mutant beta subunit purified from transgenic rice seeds exhibited high phagocytosis-stimulating activity, demonstrating its potential value in enhancing the nutritional value of rice.
authors.affiliation: Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Takayasu
authors.initials: T
authors.lastname: Motoyama
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Amari
authors.forename: Mary Rose
authors.initials: MR
authors.lastname: Tandang-Silvas
authors.forename: Cerrone
authors.initials: C
authors.lastname: Cabanos
authors.forename: Aiko
authors.initials: A
authors.lastname: Kimura
authors.forename: Masaaki
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Fumio
authors.initials: F
authors.lastname: Takaiwa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Utsumi
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Maruyama
mesh_terms: D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000235:genetics*; D005916:Globulins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics*; D010587:Phagocytosis / Q000235:genetics*; D030821:Plants, Genetically Modified; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D055314:Seed Storage Proteins / Q000235:genetics*; D030262:Soybean Proteins / Q000235:genetics*
chemical_list: D052179:Antigens, Plant; D005916:Globulins; D021122:Protein Subunits; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max