Free-floating ultrathin two-dimensional crystals from sequence-specific peptoid polymers.
The design and synthesis of protein-like polymers is a fundamental challenge in materials science. A biomimetic approach is to explore the impact of monomer sequence on non-natural polymer structure and function. We present the aqueous self-assembly of two peptoid polymers into extremely thin two-dimensional (2D) crystalline sheets directed by periodic amphiphilicity, electrostatic recognition and aromatic interactions. Peptoids are sequence-specific, oligo-N-substituted glycine polymers designed to mimic the structure and functionality of proteins. Mixing a 1:1 ratio of two oppositely charged peptoid 36mers of a specific sequence in aqueous solution results in the formation of giant, free-floating sheets with only 2.7 nm thickness. Direct visualization of aligned individual peptoid chains in the sheet structure was achieved using aberration-corrected transmission electron microscopy. Specific binding of a protein to ligand-functionalized sheets was also demonstrated. The synthetic flexibility and biocompatibility of peptoids provide a flexible and robust platform for integrating functionality into defined 2D nanostructures.
Nam
Ki Tae
KT
Molecular Foundry; National Center for Electron Microscopy, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Shelby
Sarah A
SA
Choi
Philip H
PH
Marciel
Amanda B
AB
Chen
Ritchie
R
Tan
Li
L
Chu
Tammy K
TK
Mesch
Ryan A
RA
Lee
Byoung-Chul
BC
Connolly
Michael D
MD
Kisielowski
Christian
C
Zuckermann
Ronald N
RN
D000595:Amino Acid Sequence; D032701:Biomimetics; D002021:Buffers; D003460:Crystallization; D005583:Fourier Analysis; D005998:Glycine / Q000031:analogs & derivatives*; D008024:Ligands; D008956:Models, Chemical; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D034444:Peptoids / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity; D014867:Water / Q000737:chemistry
D002021:Buffers; D008024:Ligands; D034444:Peptoids; D011108:Polymers; D014867:Water; D005998:Glycine
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