title: Generation of patterned neuronal networks on cell-repellant poly(oligo(ethylene glycol) methacrylate) films.
abstract: The utilization of non-biofouling poly(oligo(ethylene glycol) methacrylate) (pOEGMA) films as a background material for the generation of neuronal patterns is reported here. Our previously reported method, which was surface-initiated, atom transfer radical polymerization of OEGMA, and subsequent activation of terminal hydroxyl groups of pOEGMA with disuccinimidyl carbonate, was employed for the generation of activated pOEGMA films on glass. Poly-L-lysine was then microcontact-printed onto the activated polymer films, followed by backfilling with poly(ethylene glycol) moieties. E18 hippocampal neurons were cultured on the chemically patterned substrate, and the resulting neuronal networks were analyzed by phase-contrast microscopy and whole-cell patch clamp method. The results indicated that the pOEGMA films played an important role in the generation of good-quality neuronal patterns for up to two weeks without any negative effects to neurons.
authors.affiliation: Molecular-Level Interface Research Center, Department of Chemistry, KAIST, Daejeon 305-701, Korea.
authors.forename: Kyungtae
authors.initials: K
authors.lastname: Kang
authors.forename: Gyumin
authors.initials: G
authors.lastname: Kang
authors.forename: Bong Soo
authors.initials: BS
authors.lastname: Lee
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
authors.forename: Yoonkey
authors.initials: Y
authors.lastname: Nam
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D005898:Glass / Q000737:chemistry; D009415:Nerve Net / Q000166:cytology*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry*; D051381:Rats
chemical_list: D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D011107:Polylysine; D011092:Polyethylene Glycols