title: Impact of administered bifidobacterium on murine host fatty acid composition.
abstract: Recently, we reported that administration of Bifidobacteria resulted in increased concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in murine adipose tissue [1]. The objective of this study was to assess the impact of co-administration of Bifidobacterium breve NCIMB 702258 and the substrate for EPA, alpha-linolenic acid, on host fatty acid composition. alpha-Linolenic acid-supplemented diets (1%, wt/wt) were fed to mice (n = 8), with or without B. breve NCIMB 702258 (daily dose of 10(9) microorganisms) for 8 weeks. Two further groups received either supplement of B. breve alone or unsupplemented diet. Tissue fatty acid composition was assessed by gas liquid chromatography. Dietary supplementation of alpha-linolenic acid resulted in higher (P < 0.05) alpha-linolenic acid and EPA concentrations in liver and adipose tissue and lower (P < 0.05) arachidonic acid in liver, adipose tissue and brain compared with mice that did not receive alpha-linolenic acid. Supplementation with B. breve NCIMB 702258 in combination with alpha-linolenic acid resulted in elevated (P < 0.05) liver EPA concentrations compared with alpha-linolenic acid supplementation alone. Furthermore, the former group had higher (P < 0.05) DHA in brain compared with the latter group. These results suggest a role for interactions between fatty acids and commensals in the gastrointestinal tract. This interaction between administered microbes and fatty acids could result in a highly effective nutritional approach to the therapy of a variety of inflammatory and neurodegenerative conditions.
authors.affiliation: Alimentary Pharmabiotic Centre, Co Cork, Ireland.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Wall
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.forename: Fergus
authors.initials: F
authors.lastname: Shanahan
authors.forename: Liam
authors.initials: L
authors.lastname: O'Mahony
authors.forename: Barry
authors.initials: B
authors.lastname: Kiely
authors.forename: Eamonn
authors.initials: E
authors.lastname: Quigley
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Dinan
authors.forename: Gerald
authors.initials: G
authors.lastname: Fitzgerald
authors.forename: Catherine
authors.initials: C
authors.lastname: Stanton
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001644:Bifidobacterium; D001923:Brain Chemistry; D005227:Fatty Acids / Q000737:chemistry*; D005243:Feces / Q000382:microbiology; D005260:Female; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D017962:alpha-Linolenic Acid
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