Insights into multienzyme docking in hybrid PKS-NRPS megasynthetases revealed by heterologous expression and genetic engineering.
Li
Yanyan
Y
Department of Pharmaceutical Biotechnology, Saarland University, Saarbrücken, Germany.
Weissman
Kira J
KJ
Müller
Rolf
R
D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D008689:Methacrylates / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D048630:Polyketide Synthases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics; D016415:Sequence Alignment; D020606:Stigmatella aurantiaca / Q000201:enzymology / Q000235:genetics; D013844:Thiazoles / Q000378:metabolism
D001426:Bacterial Proteins; D008689:Methacrylates; D013844:Thiazoles; C030517:myxothiazol; D048630:Polyketide Synthases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase