A fiberoptic reflectance probe with multiple source-collector separations to increase the dynamic range of derived tissue optical absorption and scattering coefficients.
Measurement of tissue optical absorption and (transport) reduced scattering coefficients (mu(a) and mu(s)', respectively) is fundamental to many applications of light in medicine and biology. We report a handheld fiberoptic probe to determine these coefficients by measuring the diffuse reflectance at multiple source-collector distances, which allows for a larger dynamic range than a single source-collector separation. Diffusion theory and a priori knowledge of the spectral shape of mu(a) and mu(s)' are used in a forward model of the diffuse reflectance. The dynamic range and accuracy of this method were evaluated using Monte Carlo simulations, phantom experiments and tissues in vivo.
Kim
Anthony
A
Department of Medical Biophysics, University of Toronto and Ontario Cancer Institute/Campbell Family Institute for Cancer Research, University Health Network, 610 University Avenue, Toronto, Ontario, M5G 2M9, Canada.
Roy
Mathieu
M
Dadani
Farhan
F
Wilson
Brian C
BC
D003198:Computer Simulation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation*; D010783:Photometry / Q000295:instrumentation*; D014159:Transducers