Polarized fluorescent nanospheres.
Fluorescent beads (nanoparticles, nanospheres) are commonly used in fluorescence spectroscopy and microscopy. Due to the random distribution of dye and high dye to nanoparticle ratio, the fluorescence polarization observed from the beads is low. Therefore beads are not used for polarization study. We demonstrate that photoselective bleaching creates beads with highly polarized fluorescence. First, the beads were immobilized in a PVA polymer. Second, the beads-doped PVA film was exposed to the illumination within the dye absorption band. A progressive decrease of absorption was observed. Next, photophysical properties of photobleached and not bleached films dissolved in water were compared.
Luchowski
Rafal
R
Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Center for Commercialization of Fluorescence Technologies, 3500 Camp Bowie Boulevard, TX, Fort Worth, 76107, USA.
Gryczynski
Zygmunt
Z
Földes-Papp
Zeno
Z
Chang
Aaron
A
Borejdo
Julian
J
Sarkar
Pabak
P
Gryczynski
Ignacy
I
D000042:Absorption; D005453:Fluorescence; D005454:Fluorescence Polarization; D054118:Nanospheres / Q000737:chemistry*; D038761:Photobleaching; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D011142:Polyvinyl Alcohol