title: mRNA levels of enzymes and receptors implicated in arachidonic acid metabolism in gliomas.
abstract: BACKGROUND
  Gliomas are tumors of the central nervous system derived from glial cells. They show cellular heterogeneity and lack specific diagnostic markers. Although a possible role for the eicosanoid cascade has been suggested in glioma tumorigenesis, the relationship between enzymes and receptors implicated in arachidonic acid metabolism, with histological tumor type has not yet been determined.
  
  
  METHODS
  Quantitative real-time reverse transcription-polymerase chain reaction was performed to measure and compare transcript levels of enzymes and receptors implicated in both lipoxygenase and cyclooxygenase pathways between oligodendrogliomas, astrocytomas, glioblastomas and mixed oligoastrocytomas.
  
  
  RESULTS
  Arachidonic acid metabolism-related enzymes and receptor transcripts (i) were underexpressed in classical oligodendrogliomas compared to astrocytomas and/or glioblastomas, (ii) differed between astrocytomas and glioblastomas and (iii) had an intermediate expression in mixed oligoastrocytomas.
  
  
  CONCLUSIONS
  mRNA levels of enzymes and receptors implicated both in lipoxygenase and cyclooxygenase pathways differed significantly in gliomas according to the histological type.
authors.affiliation: Department of Pathology, CHU Dupuytren, 2 Av. Martin Luther King, 87042 Limoges, France.
authors.forename: Rafael
authors.initials: R
authors.lastname: De Armas
authors.forename: Karine
authors.initials: K
authors.lastname: Durand
authors.forename: Angélique
authors.initials: A
authors.lastname: Guillaudeau
authors.forename: Nicolas
authors.initials: N
authors.lastname: Weinbreck
authors.forename: Sandrine
authors.initials: S
authors.lastname: Robert
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Moreau
authors.forename: François
authors.initials: F
authors.lastname: Caire
authors.forename: Gisela
authors.initials: G
authors.lastname: Acosta
authors.forename: Matias
authors.initials: M
authors.lastname: Pebet
authors.forename: Alain
authors.initials: A
authors.lastname: Chaunavel
authors.forename: Benoît
authors.initials: B
authors.lastname: Marin
authors.forename: François
authors.initials: F
authors.lastname: Labrousse
authors.forename: Yves
authors.initials: Y
authors.lastname: Denizot
mesh_terms: D000328:Adult; D000368:Aged; D016718:Arachidonic Acid / Q000378:metabolism*; D016543:Central Nervous System Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D005260:Female; D005910:Glioma / Q000175:diagnosis / Q000378:metabolism*; D006801:Humans; D008084:Lipoxygenase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D011982:Receptors, Prostaglandin / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D011982:Receptors, Prostaglandin; D016718:Arachidonic Acid; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases