Brain microvascular endothelial cells mediate neuroprotective effects on ischemia/reperfusion neurons.
OBJECTIVE
The permeability of the blood-brain barrier (BBB) is a bottleneck for the development of new cerebropathy medications because the medication must be transmitted across the BBB to achieve its curative function. To explore a new approach to the treatment of brain disease, this study investigated the mediating effects of brain microvascular endothelial cells (MVECs) on injured neurons.


METHODS
MVECs and cortical neurons were cultured and damage by cerebral ischemia/reperfusion (I/R) was simulated. The conditioned media from four groups of MVECs - normal cells (N-CM), normal cells treated with Tong Luo Jiu Nao (TLJN) (NT-CM), simulated cerebral I/R cells (I/R-CM), and simulated cells treated with TLJN (I/RTCM) - were then collected. These conditioned media were added to neuronal cultures and the viability of the neurons was examined.


RESULTS
The results demonstrated that N-CM could alleviate I/R damage to neurons, and this capacity could be improved by TLJN treatment. However, I/R-CM could cause damage to normal and I/R neurons, while I/RT-CM could significantly alleviate the damage to I/R neurons.


CONCLUSIONS
We propose that MVECs secrete active substances that influence the survival of neurons, and so MVECs may mediate a neuroprotective effect on ischemia/reperfusion neurons.
Hua
Qian
Q
School of Preclinical Medicine, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China.
Qing
Xuemei
X
Li
Pengtao
P
Li
Weihong
W
Hou
Jincai
J
Hu
Jinghong
J
Hong
Qingtao
Q
Sun
Ping
P
Zhu
Xiaolei
X
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology; D002545:Brain Ischemia / Q000473:pathology / Q000517:prevention & control; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D055806:Microvessels / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D008954:Models, Biological; D015394:Molecular Structure; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000473:pathology*; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control
D017077:Culture Media, Conditioned; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C528243:tongluo jiunao