CSF chemokine alterations related to the clinical course of amyotrophic lateral sclerosis.
We measured the levels of 27 cytokines/chemokines and growth factors in cerebrospinal fluid (CSF) from 42 patients with sporadic amyotrophic lateral sclerosis (ALS), 12 patients with lower motor neuron disease (LMND), and 34 control patients with non-inflammatory neurological diseases (OND), using a multiplexed fluorescent bead-based immunoassay. Among cytokines/chemokines elevated in ALS, CCL2 and CXCL8 levels were negatively correlated with the revised ALS functional rating scale (ALSFRS-R) score, while CCL4 showed a positive correlation with ALSFRS-R score. CCL4 and CXCL10 showed negative correlations with disease progression rate. These chemokine alterations are assumed to somehow correlate with the clinical course of ALS.
Tateishi
Takahisa
T
Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Yamasaki
Ryo
R
Tanaka
Masahito
M
Matsushita
Takuya
T
Kikuchi
Hitoshi
H
Isobe
Noriko
N
Ohyagi
Yasumasa
Y
Kira
Jun-ichi
J
D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000134:cerebrospinal fluid* / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis / Q000134:cerebrospinal fluid; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis / Q000378:metabolism*; D018932:Chemokine CCL2 / Q000032:analysis / Q000134:cerebrospinal fluid; D054407:Chemokine CCL4 / Q000032:analysis / Q000134:cerebrospinal fluid; D054357:Chemokine CXCL10 / Q000032:analysis / Q000134:cerebrospinal fluid; D018925:Chemokines / Q000032:analysis / Q000134:cerebrospinal fluid*; D015536:Down-Regulation / Q000276:immunology; D005260:Female; D016231:Fluorescence Polarization Immunoassay; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis / Q000134:cerebrospinal fluid; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D015854:Up-Regulation / Q000276:immunology
D015415:Biomarkers; C111835:CCL2 protein, human; D002556:Cerebrospinal Fluid Proteins; D018932:Chemokine CCL2; D054407:Chemokine CCL4; D054357:Chemokine CXCL10; D018925:Chemokines; D016209:Interleukin-8