title: A highly selective oligopeptide binding protein from the archaeon Sulfolobus solfataricus.
abstract: SSO1273 of Sulfolobus solfataricus was identified as a cell surface-bound protein by a proteomics approach. Sequence inspection of the genome revealed that the open reading frame of sso1273 is associated in an operon-like structure with genes encoding all the remaining components of a canonical protein-dependent ATP-binding cassette (ABC) transporter. sso1273 gene expression and SSO1273 protein accumulation on the cell surface were demonstrated to be strongly induced by the addition of a peptide mixture (tryptone) to the culture medium. The native protein was obtained in multimeric form, mostly hexameric, under the purification conditions used, and it was characterized as an oligopeptide binding protein, named S. solfataricus OppA (OppA(Ss)). OppaA(Ss) possesses typical sequence patterns required for glycosylphosphatidylinositol lipid anchoring, resulting in an N-linked glycoprotein with carbohydrate moieties likely composed of high mannose and/or hybrid complex carbohydrates. OppA(Ss) specifically binds oligopeptides and shows a marked selectivity for the amino acid composition of substrates when assayed in complex peptide mixtures. Moreover, a truncated version of OppA(Ss), produced in recombinant form and including the putative binding domain, showed a low but significant oligopeptide binding activity.
authors.affiliation: IBP-Consiglio Nazionale delle Ricerche, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Gogliettino
authors.forename: M
authors.initials: M
authors.lastname: Balestrieri
authors.forename: G
authors.initials: G
authors.lastname: Pocsfalvi
authors.forename: I
authors.initials: I
authors.lastname: Fiume
authors.forename: L
authors.initials: L
authors.lastname: Natale
authors.forename: M
authors.initials: M
authors.lastname: Rossi
authors.forename: G
authors.initials: G
authors.lastname: Palmieri
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D019848:Gene Expression Regulation, Archaeal / Q000502:physiology*; D009842:Oligopeptides / Q000378:metabolism*; D011485:Protein Binding; D013379:Substrate Specificity; D048229:Sulfolobus solfataricus / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D019843:Archaeal Proteins; D009842:Oligopeptides
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