title: Characterization of the mouse CP27 promoter and NF-Y mediated gene regulation.
abstract: The cp27 gene is a highly conserved and unique gene with important roles related to craniofacial organogenesis. The present study is a first analysis of the CP27 promoter and its regulation. Here, we have cloned the promoter of the mouse cp27 gene, examined its transcriptional activity, and identified transcription factor binding sites in the proximal promoter region. Two major transcription start sites were mapped adjacent to exon 1. Promoter function analysis of the 5' flanking region by progressive 5' deletion mutations localized transcription repression elements between -1993bp and -969bp and several positive elements between -968bp and the preferred transcription start site. EMSA and functional studies indicated two function-cooperative CCAAT boxes and identified the NF-Y transcription factor as the CCAAT activator controlling transactivation of the CP27 promoter. In addition, this study demonstrated that for its effective binding and function, NF-Y required not only the minimal DNA segment length identified by deletion studies, but also a defined nucleotide sequence in the distal 3' flanking region of the CP27 proximal promoter CCAAT box. These results provide a basis for our understanding of the specific regulation of the cp27 gene in the NF-Y-mediated gene transcription network.
authors.affiliation: Brodie Laboratory for Craniofacial Genetics, University of Illinois at Chicago, 801 South Paulina Street, Chicago, IL 60612, USA.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Luan
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Youbin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Thomas G H
authors.initials: TG
authors.lastname: Diekwisch
mesh_terms: D024509:3' Flanking Region; D016475:3T3 Cells; D024506:5' Flanking Region; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D023081:CCAAT-Binding Factor / Q000235:genetics*; D002874:Chromosome Mapping; D016326:Extracellular Matrix Proteins; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D024363:Transcription Initiation Site
chemical_list: D023081:CCAAT-Binding Factor; C121022:Cfdp1 protein, mouse; D016326:Extracellular Matrix Proteins; D009687:Nuclear Proteins; D011506:Proteins; C402244:nuclear factor Y
references.citation: Mech Dev. 1997 Oct;67(2):111-23
references.pmid: 9392510
references.citation: J Biol Chem. 1997 Oct 17;272(42):26562-72
references.pmid: 9334236
references.citation: Mol Biol Evol. 2007 Mar;24(3):814-26
references.pmid: 17205977
references.citation: Nucleic Acids Res. 1998 Mar 1;26(5):1135-43
references.pmid: 9469818
references.citation: Gene. 1999 Oct 18;239(1):15-27
references.pmid: 10571030
references.citation: J Biol Chem. 1997 Jan 31;272(5):2801-7
references.pmid: 9006920
references.citation: J Biol Chem. 1999 Aug 13;274(33):23242-8
references.pmid: 10438498
references.citation: J Histochem Cytochem. 2002 Apr;50(4):583-6
references.pmid: 11897812
references.citation: PLoS One. 2008 Apr 23;3(4):e2047
references.pmid: 18431504
references.citation: Mol Cell Biol. 2008 Mar;28(6):2047-58
references.pmid: 18212061
references.citation: Cell Prolif. 2002 Aug;35(4):207-19
references.pmid: 12153613
references.citation: J Biol Chem. 2003 Aug 15;278(33):30435-40
references.pmid: 12771133
references.citation: J Biol Chem. 1995 Apr 14;270(15):8851-9
references.pmid: 7721793
references.citation: Cancer Res. 2003 Dec 1;63(23):8167-72
references.pmid: 14678971
references.citation: Gene. 1999 Jul 22;235(1-2):19-30
references.pmid: 10415329
references.citation: J Biol Chem. 1994 Aug 5;269(31):20119-25
references.pmid: 8051100
references.citation: Adv Dent Res. 1995 Nov;9(3 Suppl):12
references.pmid: 8934940
references.citation: J Biol Chem. 1992 Apr 25;267(12):8478-84
references.pmid: 1569098
references.citation: PLoS One. 2008 Apr 30;3(4):e2066
references.pmid: 18446193
references.citation: J Biol Chem. 2009 Jan 30;284(5):3136-3147
references.pmid: 19047043
references.citation: J Biol Chem. 2000 Apr 21;275(16):12273-80
references.pmid: 10766866
references.citation: Development. 1993 Jul;118(3):665-90
references.pmid: 7915668
references.citation: Anat Rec. 1996 Jun;245(2):131-50
references.pmid: 8769659
references.citation: Genome Res. 2003 May;13(5):773-80
references.pmid: 12727897
references.citation: Biochem J. 1991 Dec 15;280 ( Pt 3):761-7
references.pmid: 1764039
references.citation: J Biol Chem. 2005 Apr 8;280(14):13606-15
references.pmid: 15647281
references.citation: Nucleic Acids Res. 2006;34(21):6272-85
references.pmid: 17098936
references.citation: J Biol Chem. 2003 Jan 10;278(2):1336-45
references.pmid: 12401788
references.citation: J Biol Chem. 2003 Feb 28;278(9):6642-50
references.pmid: 12482752
references.citation: J Biol Chem. 2000 Jan 28;275(4):2979-85
references.pmid: 10644769
references.citation: Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):3948-53
references.pmid: 10759542
references.citation: Gene. 2008 Apr 30;413(1-2):42-8
references.pmid: 18316164
references.citation: EMBO J. 1998 Nov 2;17(21):6300-15
references.pmid: 9799238
references.citation: Nucleic Acids Res. 2008 Nov;36(20):6592-607
references.pmid: 18940868
references.citation: J Biol Chem. 2006 Jun 9;281(23):16090-8
references.pmid: 16595692
references.citation: Cancer Cell. 2006 Sep;10(3):191-202
references.pmid: 16959611
references.citation: Biochem Biophys Res Commun. 1999 Nov 19;265(2):439-47
references.pmid: 10558886
references.citation: Oncogene. 2004 May 20;23(23):4084-8
references.pmid: 15064732
references.citation: EMBO J. 1995 Sep 15;14(18):4514-22
references.pmid: 7556094
references.citation: Nucleic Acids Res. 1992 Apr 11;20(7):1769-75
references.pmid: 1349743
references.citation: Gene. 2003 Jul 17;312:225-37
references.pmid: 12909359
references.citation: Gene Expr. 2005;12(3):193-212
references.pmid: 16128003
references.citation: FEBS Lett. 2003 Feb 27;537(1-3):58-62
references.pmid: 12606031
references.citation: Brain Res Mol Brain Res. 1999 Nov 10;73(1-2):172-80
references.pmid: 10581410
references.citation: Biotechniques. 1995 Jul;19(1):54-5
references.pmid: 7669296
references.citation: J Biol Chem. 2008 Jul 11;283(28):19359-70
references.pmid: 18424784
references.citation: J Biol Chem. 1999 Nov 26;274(48):34327-36
references.pmid: 10567409
references.citation: Biochem Biophys Res Commun. 2009 Feb 6;379(2):356-61
references.pmid: 19103169
references.citation: Cell. 1987 Sep 11;50(6):863-72
references.pmid: 3476205
references.citation: Proc Natl Acad Sci U S A. 1988 Oct;85(19):7322-6
references.pmid: 3174636
references.citation: Mol Cell Biol. 2005 May;25(9):3737-51
references.pmid: 15831478
references.citation: Gene. 2002 Apr 3;287(1-2):141-7
references.pmid: 11992732