title: Proteomic profiling of human bone marrow mesenchymal stem cells under shear stress.
abstract: Mesenchymal stem cells (MSCs) are promising seed cells for tissue engineering of blood vessels. As seed cells, MSCs must endure blood fluid shear stress after transplantation. It has been shown that fluid shear stress can regulate the proliferation and differentiation of MSCs. However, the effects of fluid shear stress on MSCs including the types of proteins modulated are still not well understood. In this study, we exposed human mesenchymal stem cells (HMSCs) to 3 dyn/cm(2) shear stress for 6 h and compared them to a control group using proteomic analysis. Thirteen specific proteins were affected by shear stress, 10 of which were up-regulated. Shear stress especially induced sustained increases in the expression of Annexin A2 and GAPDH, which have been specifically shown to affect HMSCs function. We present here the first comparative proteome analysis of effect of shear stress on HMSCs.
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, 4th Military Medical University, 127 Changle West Road, Xi'an 710032, Shaanxi, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yi
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.forename: Xufeng
authors.initials: X
authors.lastname: Wei
authors.forename: Chunhu
authors.initials: C
authors.lastname: Gu
authors.forename: Xiaochao
authors.initials: X
authors.lastname: Dong
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Kang
authors.forename: Shuzhong
authors.initials: S
authors.lastname: Guo
authors.forename: Kefeng
authors.initials: K
authors.lastname: Dou
mesh_terms: D017306:Annexin A2 / Q000032:analysis / Q000096:biosynthesis*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods; D015854:Up-Regulation
chemical_list: C472822:ANXA2 protein, human; D017306:Annexin A2; D011506:Proteins; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
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