Genetic transformation of the codling moth, Cydia pomonella L., with piggyBac EGFP.
Genetic transformation of the codling moth, Cydia pomonella, was accomplished through embryo microinjection with a plasmid-based piggyBac vector containing the enhanced green fluorescent protein (EGFP) gene. Sequencing of the flanking regions around the inserted construct resulted in identification of insect genomic sequences, not plasmid sequences, thus providing evidence that the piggyBac EGFP cassette had integrated into the codling moth genome. EGFP-positive moths were confirmed in the 28th and earlier generations post injection through PCR and Southern blot analyses, indicating heritability of the transgene.
Ferguson
Holly J
HJ
Washington State University, 24106 North Bunn Road, Prosser, WA 99350, USA. hferguson@wsu.edu
Neven
Lisa G
LG
Thibault
Stephen T
ST
Mohammed
Ahmed
A
Fraser
Malcolm
M
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004625:Embryo, Nonmammalian; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D008845:Microinjections; D009036:Moths / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D014170:Transformation, Genetic; D019076:Transgenes
D004251:DNA Transposable Elements; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
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