Effect of the medium replacement interval on the viability, growth and in vitro maturation of isolated caprine and ovine pre-antral follicles.
The aim of the present study was to evaluate the effects of different medium replacement intervals on the viability, antral cavity formation, growth and in vitro maturation (IVM) of oocytes from caprine and ovine pre-antral follicles. Pre-antral ovarian follicles (≥ 150 μm) were isolated from the ovarian cortex of goats and sheep and were individually cultured for 24 days using two different medium replacement intervals [2 days (T(1) ) or 6 days (T(2) )]. Follicle development was evaluated on the basis of antral cavity formation, increases in follicular diameter and the presence of healthy cumulus oocyte complexes and fully grown oocytes. For caprine species, results showed a higher percentage (p<0.05) of viable follicles in T(1) than T(2) from day 6 until the end of the culture. In addition, when comparing both treatments after the same culture duration, the rate of antrum formation was significantly higher in T(1) than in T(2) from day 12 onwards. Yet, in ovines, when both treatments were compared on day 24 of the culture, there were more viable follicles in T(2) than in T(1) (p<0.05). In the caprine species, percentages of fully grown oocytes (≥ 110 μm) acceptable for IVM after 24 days of culture were significantly higher in normal follicles cultured in T(1) (30.0%) than in T(2) (6.7%; p<0.05). On the other hand, in ovines, at the end of the culture, the percentage of oocytes destined for IVM was higher in T(2) than in T(1) (23.5% vs 2.9%; p<0.05). In conclusion, under the same conditions, the frequency of medium replacement significantly affected the in vitro development of caprine and ovine pre-antral follicles. To improve the efficiency of the culture system, the medium must be replaced every 2 and 6 days for goat and sheep pre-antral follicles, respectively.
Magalhães
D M
DM
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, CE, Brazil. dmmvet@hotmail.com
Fernandes
D D
DD
Mororó
M B S
MB
Silva
C M G
CM
Rodrigues
G Q
GQ
Bruno
J B
JB
Matos
M H T
MH
Campello
C C
CC
Figueiredo
J R
JR
D000818:Animals; D003470:Culture Media; D005260:Female; D006041:Goats; D008540:Meiosis; D009865:Oocytes / Q000166:cytology; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D012756:Sheep; D013045:Species Specificity; D046509:Tissue Culture Techniques / Q000379:methods / Q000662:veterinary
D003470:Culture Media