title: Alkaloids from roots of Stephania rotunda and their cholinesterase inhibitory activity.
abstract: In the course of screening plants used in folk medicine as memory enhancers, a 70% ethanolic extract of Stephania rotunda roots showed significant AChE inhibitory activity. Repeated column chromatography led to the isolation of a new protoberberine alkaloid, which we named stepharotudine (1), and seven known compounds (2-8). The chemical structures of the isolated compounds were elucidated based on extensive 1D and 2D NMR spectroscopic data. Compounds 1-8 were investigated in vitro for their anticholinesterase activity using a rat cortex AChE enzyme.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongbuk, Korea.
authors.forename: Tran Manh
authors.initials: TM
authors.lastname: Hung
authors.forename: Nguyen Hai
authors.initials: NH
authors.lastname: Dang
authors.forename: Jin Cheol
authors.initials: JC
authors.lastname: Kim
authors.forename: Han-Su
authors.initials: HS
authors.lastname: Jang
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Ryoo
authors.forename: Jeong Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
authors.forename: Kihwan
authors.initials: K
authors.lastname: Bae
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
mesh_terms: D000110:Acetylcholinesterase / Q000302:isolation & purification; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001600:Berberine Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001921:Brain / Q000201:enzymology; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D058851:GPI-Linked Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D031607:Stephania / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D001600:Berberine Alkaloids; D002800:Cholinesterase Inhibitors; D058851:GPI-Linked Proteins; C557381:stepharotudine; D000110:Acetylcholinesterase; C548246:Ache protein, rat