title: Multisite study of cryptosporidiosis in children with diarrhea in India.
abstract: Cryptosporidium spp., a common cause of diarrhea in children, were investigated in the first multisite study in India. Diarrheal stools from hospitalized children aged <5 years from Delhi, Trichy, and Vellore were analyzed by microscopy, PCR-restriction fragment length polymorphism (RFLP), and/or sequencing at the small-subunit (SSU) rRNA and Cpgp40/15 loci for species determination and subgenotyping, respectively. Seventy of 2,579 (2.7%) children, 75% of whom were <2 years old, had cryptosporidial diarrhea as determined by microscopy. Genotyping and subgenotyping showed that Cryptosporidium hominis was the most commonly identified species (59/67 children), and subgenotypes Ie, Ia, Ib, and Id were common in all centers. A novel C. parvum subgenotype, IIn, was identified in Vellore. Meteorological analysis revealed a higher rate of cryptosporidial positivity during hotter and drier weather in Delhi.
authors.affiliation: Department of Gastrointestinal Sciences, Christian Medical College, Vellore 632004, India.
authors.forename: Sitara Swarna Rao
authors.initials: SS
authors.lastname: Ajjampur
authors.forename: Farzana Begum
authors.initials: FB
authors.lastname: Liakath
authors.forename: Arun
authors.initials: A
authors.lastname: Kannan
authors.forename: Priya
authors.initials: P
authors.lastname: Rajendran
authors.forename: Rajiv
authors.initials: R
authors.lastname: Sarkar
authors.forename: Prabhakar Devarajan
authors.initials: PD
authors.lastname: Moses
authors.forename: Anna
authors.initials: A
authors.lastname: Simon
authors.forename: Indira
authors.initials: I
authors.lastname: Agarwal
authors.forename: Ann
authors.initials: A
authors.lastname: Mathew
authors.forename: Roberta
authors.initials: R
authors.lastname: O'Connor
authors.forename: Honorine
authors.initials: H
authors.lastname: Ward
authors.forename: Gagandeep
authors.initials: G
authors.lastname: Kang
mesh_terms: D002675:Child, Preschool; D002980:Climate; D016000:Cluster Analysis; D003457:Cryptosporidiosis / Q000453:epidemiology* / Q000469:parasitology; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology* / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D005260:Female; D020459:Genes, rRNA; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D008853:Microscopy; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S
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