title: Small RNA expression and strain specificity in the rat.
abstract: BACKGROUND
  Digital gene expression (DGE) profiling has become an established tool to study RNA expression. Here, we provide an in-depth analysis of small RNA DGE profiles from two different rat strains (BN-Lx and SHR) from six different rat tissues (spleen, liver, brain, testis, heart, kidney). We describe the expression patterns of known and novel micro (mi)RNAs and piwi-interacting (pi)RNAs.
  
  
  RESULTS
  We confirmed the expression of 588 known miRNAs (54 in antisense orientation) and identified 56 miRNAs homologous to known human or mouse miRNAs, as well as 45 new rat miRNAs. Furthermore, we confirmed specific A to I editing in brain for mir-376a/b/c and identified mir-377 as a novel editing target. In accordance with earlier findings, we observed a highly tissue-specific expression pattern for all tissues analyzed. The brain was found to express the highest number of tissue-specific miRNAs, followed by testis. Notably, our experiments also revealed robust strain-specific differential miRNA expression in the liver that is caused by genetic variation between the strains. Finally, we identified two types of germline-specific piRNAs in testis, mapping either to transposons or in strand-specific clusters.
  
  
  CONCLUSIONS
  Taken together, the small RNA compendium described here advances the annotation of small RNAs in the rat genome. Strain and tissue-specific expression patterns furthermore provide a strong basis for studying the role of small RNAs in regulatory networks as well as biological process like physiology and neurobiology that are extensively studied in this model system.
authors.affiliation: Hubrecht Institute-KNAW & University Medical Center Utrecht, Cancer Genomics Center, Utrecht, The Netherlands.
authors.forename: Sam E V
authors.initials: SE
authors.lastname: Linsen
authors.forename: Elzo
authors.initials: E
authors.lastname: de Wit
authors.forename: Ewart
authors.initials: E
authors.lastname: de Bruijn
authors.forename: Edwin
authors.initials: E
authors.lastname: Cuppen
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D035683:MicroRNAs / Q000032:analysis*; D009928:Organ Specificity; D034741:RNA, Small Interfering / Q000032:analysis*; D051381:Rats / Q000145:classification / Q000235:genetics*; D013045:Species Specificity
chemical_list: D035683:MicroRNAs; D034741:RNA, Small Interfering
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