Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins.
The rate at which X-ray structures of membrane proteins are solved is on a par with that of soluble proteins in the late 1970s. There are still many obstacles facing the membrane protein structural community. Recently, there have been several technical achievements in the field that have started to dramatically accelerate structural studies. Here, we summarize these so-called 'tricks-of-the-trade' and include case studies of several mammalian transporters.
Sonoda
Yo
Y
Division of Molecular Biosciences, Imperial College London, London, UK.
Cameron
Alex
A
Newstead
Simon
S
Omote
Hiroshi
H
Moriyama
Yoshinori
Y
Kasahara
Michihiro
M
Iwata
So
S
Drew
David
D
D000818:Animals; D003460:Crystallization; D003902:Detergents; D051272:Glucose Transporter Type 1 / Q000737:chemistry / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D055550:Protein Stability; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics; D050599:Vesicular Glutamate Transport Protein 2 / Q000737:chemistry / Q000235:genetics
D003902:Detergents; D051272:Glucose Transporter Type 1; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C495677:SLC2A1 protein, human; C493475:Slc17a6 protein, rat; D050599:Vesicular Glutamate Transport Protein 2; D049452:Green Fluorescent Proteins