Vitamin D does not modulate NF-kappaB activity in Jurkat T cells.
The active form of vitamin D, 1alpha,25-dihydroxyvitamin D(3) [1alpha,25(OH)(2)D(3)], has been reported to influence the functioning of the immune system by targeting the activities of cellular signaling pathways, in addition to its direct genomic effects. One of the signaling pathways reported to be targeted by vitamin D is the NF-kappaB pathway, which is highly active in most immune cell types, including T cells. However, the effects of vitamin D on the NF-kappaB pathway in T cells are not fully understood. Therefore, we examined the effects of 1alpha,25(OH)(2)D(3) on the NF-kappaB pathway in the Jurkat cell line, a human T cell line that constitutively expresses endogenous vitamin D receptor. We found that 1alpha,25(OH)(2)D(3) does not inhibit the induction of IkappaBalpha degradation and the expression of an NF-kappaB-dependent reporter gene in Jurkat cells following treatment with PMA/ionomycin. Also, 1alpha,25(OH)(2)D(3) did not suppress the activation of NF-kappaB by TNFalpha or PHA. Furthermore, we demonstrate that 1alpha,25(OH)(2)D(3) does not block the induction of CD69, which is an NF-kappaB target gene and an early T cell activation marker. Therefore, we conclude that vitamin D does not modulate the activity of the NF-kappaB pathway in Jurkat cells.
Shifera
Amde Selassie
AS
Department of Internal Medicine, Albert Einstein College of Medicine, Bronx, NY, USA. shiferaa@vision.ucsf.edu <shiferaa@vision.ucsf.edu>
Leong
Deborah
D
Hardin
John A
JA
D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D006801:Humans; D056747:Immunomodulation; D019169:Jurkat Cells; D037181:Lectins, C-Type / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D014807:Vitamin D / Q000031:analogs & derivatives / Q000494:pharmacology*; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015415:Biomarkers; D037181:Lectins, C-Type; D016328:NF-kappa B; D014807:Vitamin D; D000099192:CD69 Antigens
J Exp Med. 2002 Mar 4;195(5):603-16
11877483
Physiol Rev. 1998 Oct;78(4):1193-231
9790574
Nephrol Dial Transplant. 2006 Apr;21(4):889-97
16455676
J Biol Chem. 1995 Sep 15;270(37):21545-51
7665567
Biochem Biophys Res Commun. 1996 Sep 24;226(3):695-702
8831677
EMBO J. 1996 Nov 15;15(22):6189-96
8947041
Exp Cell Res. 2002 Jan 15;272(2):176-84
11777342
J Immunol. 2007 Jan 1;178(1):145-53
17182549
J Nutr. 2004 Nov;134(11):2948-52
15514257
J Neurosci Res. 1998 Sep 15;53(6):742-6
9753201
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4990-4
6946446
Am J Physiol Endocrinol Metab. 2006 Aug;291(2):E315-22
16507601
J Steroid Biochem Mol Biol. 2009 Jan;113(1-2):134-8
19138739
Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):10990-4
7479923
FEBS Lett. 2007 May 1;581(9):1751-7
17418144
Biochem Biophys Res Commun. 2002 Sep 27;297(3):645-52
12270143
J Immunol. 1998 Aug 15;161(4):1743-50
9712039
Oncogene. 2000 Mar 9;19(11):1448-56
10723136
Annu Rev Nutr. 2003;23:117-45
12651965
Circulation. 1997 Sep 16;96(6):1755-60
9323058
Am J Physiol Endocrinol Metab. 2004 Apr;286(4):E598-608
14665442
In Vitro Cell Dev Biol Anim. 2005 Mar-Apr;41(3-4):80-2
16029077
J Steroid Biochem Mol Biol. 2005 Oct;97(1-2):93-101
16046118
Eur J Immunol. 2007 Feb;37(2):395-405
17274004
J Immunol. 2009 Nov 1;183(9):5458-67
19843932
Life Sci. 2001 Jul 20;69(9):1067-77
11508649
Cell Immunol. 2004 Nov-Dec;232(1-2):137-43
15876428
J Cell Biochem. 2004 Jul 1;92(4):833-48
15211579
J Biol Chem. 1997 Nov 28;272(48):30412-20
9374532
Oncogene. 1997 Jun 12;14(23):2845-55
9190901
Annu Rev Immunol. 2009;27:693-733
19302050
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5907-11
6577459
J Immunol. 1992 May 15;148(10):3152-7
1315830
J Am Soc Nephrol. 2001 Jun;12(6):1188-1196
11373341
Exp Hematol. 2002 Apr;30(4):285-96
11937262
Arch Dermatol Res. 1999 Sep;291(9):500-6
10541880
J Biol Chem. 1998 Apr 24;273(17):10338-48
9553089
J Immunol. 1997 Dec 1;159(11):5463-73
9580241
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):16007-12
16239345
Mol Cell Biol. 1999 Jun;19(6):4191-9
10330159
Arch Biochem Biophys. 2007 Apr 15;460(2):218-26
17367745
Arch Biochem Biophys. 2009 Oct 15;490(2):145-57
19706284
Am J Respir Cell Mol Biol. 1998 Aug;19(2):259-68
9698598
Biochim Biophys Acta. 1995 Jul 25;1263(1):1-9
7632726
J Immunol. 1998 Jan 1;160(1):209-18
9551973
J Clin Endocrinol Metab. 1983 Dec;57(6):1308-10
6313738
Mol Immunol. 2008 Mar;45(6):1633-45
18207244
Biochem Biophys Res Commun. 1982 Jun 15;106(3):1012-8
6288027
Int J Cancer. 2001 Jul 15;93(2):224-31
11410870
Mol Cell Biol. 1993 Jun;13(6):3301-10
8497253
Crit Rev Eukaryot Gene Expr. 1998;8(1):19-42
9673449
Lab Invest. 2007 Jun;87(6):540-7
17401435
J Biol Chem. 2004 Jul 9;279(28):28827-30
15084594