title: Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores.
abstract: Engineered protein pores have several potential applications in biotechnology: as sensor elements in stochastic detection and ultrarapid DNA sequencing, as nanoreactors to observe single-molecule chemistry, and in the construction of nano- and micro-devices. One important class of pores contains molecular adapters, which provide internal binding sites for small molecules. Mutants of the alpha-hemolysin (alphaHL) pore that bind the adapter beta-cyclodextrin (betaCD) approximately 10(4) times more tightly than the wild type have been obtained. We now use single-channel electrical recording, protein engineering including unnatural amino acid mutagenesis, and high-resolution x-ray crystallography to provide definitive structural information on these engineered protein nanopores in unparalleled detail.
authors.affiliation: Department of Chemistry, University of Oxford, Oxford, OX1 3TA, United Kingdom.
authors.forename: Arijit
authors.initials: A
authors.lastname: Banerjee
authors.forename: Ellina
authors.initials: E
authors.lastname: Mikhailova
authors.forename: Stephen
authors.initials: S
authors.lastname: Cheley
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Gu
authors.forename: Michelle
authors.initials: M
authors.lastname: Montoya
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Nagaoka
authors.forename: Eric
authors.initials: E
authors.lastname: Gouaux
authors.forename: Hagan
authors.initials: H
authors.lastname: Bayley
mesh_terms: D018360:Crystallography, X-Ray; D003505:Cyclodextrins / Q000737:chemistry*; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000235:genetics; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008958:Models, Molecular; D009154:Mutation; D049329:Nanostructures / Q000737:chemistry*; D016062:Porosity; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013816:Thermodynamics
chemical_list: D003505:Cyclodextrins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins
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