Association of interleukin-10, interferon-gamma, transforming growth factor-beta, and tumor necrosis factor-alpha gene polymorphisms with long-term kidney allograft survival.
BACKGROUND
Single nucleotide polymorphisms within promoter or other regulatory sequences of cytokine genes mainly influence the level of production and secretion of proteins. A large amount of evidence has shown that cytokine gene variations alter graft survival length after kidney transplantation. We studied the association of gene polymorphisms in the interlekin-10 gene (IL10; -1082 G/A), interferon-gamma gene (IFNG; +874 T/A), transforming growth factor-beta gene (TGFB; +869 T/C), and tumor necrosis factor-alpha gene (TNFA; -308 A/G) with kidney allograft survival.


METHODS
The IL10 (-1082 G/A), IFNG (+874 T/A), TGFB (+869 T/C), and TNFA (-308 A/G) genotypes were determined in 32 kidney allograft recipients with graft rejection during the 1st posttransplant year and 52 without rejection in 5 posttransplant years, using allele-specific oligonucleotides-polymerase chain reaction method.


RESULTS
The IFNG +874 A/T genotype showed a significantly higher frequency among kidney recipients of the rejection group than the control group (odds ratio, 2.64, 95% confidence interval, 1.03 to 6.74; P = .04). Comparisons between the rejection and control groups in IL10 (-1082 G/A), IFNG (+874 T/A), TGFB (+869 T/C), and TNFA (-308 A/G) single nucleotide polymorphisms showed no significant difference.


CONCLUSIONS
Based on the finding of this study, it seems polymorphisms in the genes that regulate IL-10, IFN-gamma, TGF-beta, and TNF-alpha cytokines do not play a major role in kidney allograft survival, and other potential factors in this regard should be considered.
Omrani
Mir Davood
MD
Deptartment of Genetics, Urmia University of Medical Sciences, Urmia, Iran. davood_omrani@yahoo.co.uk
Mokhtari
Mohammad Reza
MR
Bagheri
Morteza
M
Ahmadpoor
Pedram
P
D006084:Graft Rejection / Q000235:genetics*; D006085:Graft Survival / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics; D016030:Kidney Transplantation / Q000009:adverse effects*; D020641:Polymorphism, Single Nucleotide; D016212:Transforming Growth Factor beta / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
C508609:IL10 protein, human; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma