Estimation of pullulan by hydrolysis with pullulanase.
A novel method for the estimation of pullulan was developed in which pullulan was hydrolysed by pullulanase. The hydrolysed product was mainly maltotriose and was determined colorimetrically using 3,5-dimethylsalicylic acid. This gave good linearity with respect to the concentration of pullulan in the fermentation broth. The content of pullulan determined in this way was less than that determined by a coupled enzyme assay and was comparable to that determined by an HPLC method. The new method was specific for estimation of pullulan, demonstrated high accuracy, and could assay pullulan from up to 3.2 mg/ml.
Wu
Sheng-Jun
SJ
School of Food Engineering, HuaiHai Institute of Technology, Lianyungang, China.
Kim
Jin-Moon
JM
Zhou
Chao
C
Jin
Zheng-Yu
ZY
Tong
Qun-Yi
QY
D002851:Chromatography, High Pressure Liquid; D057075:Enzyme Assays / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D005936:Glucans / Q000032:analysis* / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D015394:Molecular Structure; D012372:ROC Curve; D013213:Starch / Q000737:chemistry
D005936:Glucans; D000431:Ethanol; C009109:pullulan; D013213:Starch; D006026:Glycoside Hydrolases; C100181:pullulanase