title: Proteomic analysis of Escherichia coli with experimentally induced resistance to piperacillin/tazobactam.
abstract: The worldwide emergence of antibiotic-resistant bacteria poses a serious threat to human health. In addition to the difficulties in controlling infectious diseases, the phenotype of resistance can generate metabolic changes which, in turn, can interfere with host-pathogen interactions. The aim of the present study was to identify changes in the subproteome of a laboratory-derived piperacillin/tazobactam-resistant strain of Escherichia coli (minimal inhibitory concentration [MIC] = 128 mg/L) as compared with its susceptible wild-type strain E. coli ATCC 25922 (MIC = 2 mg/L) using 2-D fluorescence difference gel electrophoresis (2D-DIGE) followed by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF MS). In the resistant strain, a total of 12 protein species were increased in abundance relative to the wild-type strain, including those related to bacterial virulence, antibiotic resistance and DNA protection during stress. Fourteen proteins were increased in abundance in the wild-type strain compared to the resistant strain, including those involved in glycolysis, protein biosynthesis, pentose-phosphate shunt, amino acid transport, cell division and oxidative stress response. In conclusion, our data show overall changes in the subproteome of the piperacillin/tazobactam-resistant strain, reporting for the first time the potential role of a multidrug efflux pump system in E. coli resistance to piperacillin/tazobactam.
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Caixa Postal 486, 30.161-970 Belo Horizonte MG, Brazil. keniavaleria@gmail.com
authors.forename: Kênia Valéria
authors.initials: KV
authors.lastname: dos Santos
authors.forename: Cláudio Galuppo
authors.initials: CG
authors.lastname: Diniz
authors.forename: Luciano de Castro
authors.initials: Lde C
authors.lastname: Veloso
authors.forename: Hélida Monteiro
authors.initials: HM
authors.lastname: de Andrade
authors.forename: Mario da Silva
authors.initials: Mda S
authors.lastname: Giusta
authors.forename: Simone da Fonseca
authors.initials: Sda F
authors.lastname: Pires
authors.forename: Agenor Valadares
authors.initials: AV
authors.lastname: Santos
authors.forename: Ana Carolina Morais
authors.initials: AC
authors.lastname: Apolônio
authors.forename: Maria Auxiliadora Roque
authors.initials: MA
authors.lastname: de Carvalho
authors.forename: Luiz de Macêdo
authors.initials: Lde M
authors.lastname: Farias
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D010878:Piperacillin / Q000494:pharmacology*; D040901:Proteomics; D000078142:Tazobactam
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D010397:Penicillanic Acid; D000078142:Tazobactam; D010878:Piperacillin