Effect of certain elicitors on production of pyrrolizidine alkaloids in hairy root cultures of Echium rauwolfii.
Hairy root cultures of Echium rauwolfii were obtained by infection of sterile apical shoots with Agrobacterium rhizogenes. The linear increase in fresh weight was found to be parallel to the alkaloids production. The transformed cultures were exposed to different elicitors, such as methyl jasmonate (MJ), quercetin and salicylic acid in order to increase their productivity. Pyrrolizidine alkaloids were quantitatively determined by HPLC. Estimation of total alkaloids was achieved by peak area calculations. MJ at a concentration of 100 microM induced the accumulation of total alkaloids about 19-fold compared to the untreated control. The flavonoid quercetin (Q) at a concentration of 50 microM enhanced the pyrrolizidine accumulation approximately 6-fold. The induction effect of both MJ and Q can be suppressed by pre-incubation of hairy root cultures with salicylic acid.
Abd El-Mawla
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AM
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt. Ahmedpha2000@yahoo.com
D000085:Acetates / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003517:Cyclopentanes / Q000494:pharmacology*; D036661:Echium / Q000378:metabolism*; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000378:metabolism; D011763:Pyrrolizidine Alkaloids / Q000032:analysis / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology*; D020156:Salicylic Acid / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D013268:Stimulation, Chemical
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D011763:Pyrrolizidine Alkaloids; C072239:methyl jasmonate; D011794:Quercetin; D020156:Salicylic Acid