mRNA levels of enzymes and receptors implicated in arachidonic acid metabolism in gliomas.
BACKGROUND
Gliomas are tumors of the central nervous system derived from glial cells. They show cellular heterogeneity and lack specific diagnostic markers. Although a possible role for the eicosanoid cascade has been suggested in glioma tumorigenesis, the relationship between enzymes and receptors implicated in arachidonic acid metabolism, with histological tumor type has not yet been determined.


METHODS
Quantitative real-time reverse transcription-polymerase chain reaction was performed to measure and compare transcript levels of enzymes and receptors implicated in both lipoxygenase and cyclooxygenase pathways between oligodendrogliomas, astrocytomas, glioblastomas and mixed oligoastrocytomas.


RESULTS
Arachidonic acid metabolism-related enzymes and receptor transcripts (i) were underexpressed in classical oligodendrogliomas compared to astrocytomas and/or glioblastomas, (ii) differed between astrocytomas and glioblastomas and (iii) had an intermediate expression in mixed oligoastrocytomas.


CONCLUSIONS
mRNA levels of enzymes and receptors implicated both in lipoxygenase and cyclooxygenase pathways differed significantly in gliomas according to the histological type.
De Armas
Rafael
R
Department of Pathology, CHU Dupuytren, 2 Av. Martin Luther King, 87042 Limoges, France.
Durand
Karine
K
Guillaudeau
Angélique
A
Weinbreck
Nicolas
N
Robert
Sandrine
S
Moreau
Jean-Jacques
JJ
Caire
François
F
Acosta
Gisela
G
Pebet
Matias
M
Chaunavel
Alain
A
Marin
Benoît
B
Labrousse
François
F
Denizot
Yves
Y
D000328:Adult; D000368:Aged; D016718:Arachidonic Acid / Q000378:metabolism*; D016543:Central Nervous System Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D005260:Female; D005910:Glioma / Q000175:diagnosis / Q000378:metabolism*; D006801:Humans; D008084:Lipoxygenase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D011982:Receptors, Prostaglandin / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D011982:Receptors, Prostaglandin; D016718:Arachidonic Acid; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases