Methodological guidelines for genetic safety testing of cell transplants.
A combination of karyotyping and aneuploidy analysis by interphase fluorescent in situ hybridization is a sensitive method for evaluation of genetic stability of stem cell cultures. The methodology and specific features of preparing and analyzing the cytogenetic preparations are described as exemplified by human multipotent mesenchymal stromal cells.
Bochkov
N P
NP
Laboratory of Mutagenesis, Medical Genetic Center, Russian Academy of Medical Sciences, Russia.
Nikitina
V A
VA
Voronina
E S
ES
Kuleshov
N P
NP
D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D003582:Cytogenetics / Q000379:methods*; D017408:Guidelines as Topic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping / Q000379:methods*; D045164:Mesenchymal Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods; D039902:Multipotent Stem Cells / Q000166:cytology / Q000502:physiology; D011003:Ploidies; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology