Quantification of taurine and amino acids in mice single fibrosarcoma cell by microchip electrophoresis coupled with chemiluminescence detection.
A method based on MCE coupled with chemiluminescence (CL) detection was developed for the determination of taurine (Tau) and amino acids including alanine (Ala), glycine (Gly), tryptophan (Trp), glutamic acid (Glu) and aspartic acid (Asp) present in mice single fibrosarcoma (S180) cells. Cell injection, loading, cytolysis, electrophoretic separation and CL detection were integrated onto a simple double-T microfluidic chip. The intracellular constituents were electrophoretically separated within 150 s. The CL detection was based on the enhancement effects of Tau and amino acids on the CL reaction of luminol with H(2)O(2) and Cu(2+). The average amounts of Tau, Trp, Gly, Ala, Glu and Asp in per S180 cell from a cell population were 4.73, 1.23, 2.65, 1.94, 1.61 and 1.99 fmol. Ten S180 cells were analyzed, and the contents of Tau, Trp, Gly, Ala, Glu and Asp in mice single S180 cells were found to be in the range of 1.78-8.84, 0.95-2.31, 1.08-6.87, 1.03-4.05, 0.84-2.61 and 0.82-3.68 fmol, respectively. This work demonstrates that MCE coupled with CL detection is a useful analytical tool that is simple, quick and highly sensitive for single-cell analysis.
Ye
Fanggui
F
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, PR China.
Huang
Yong
Y
Xu
Qing
Q
Shi
Ming
M
Zhao
Shulin
S
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D005354:Fibrosarcoma / Q000737:chemistry*; D016014:Linear Models; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D013654:Taurine / Q000032:analysis*
D000596:Amino Acids; D013654:Taurine