title: APOE genotype is associated with oral herpetic lesions but not genital or oral herpes simplex virus shedding.
abstract: BACKGROUND
  Apolipoprotein E is polymorphic in the human population. APOE4 has previously been reported to correlate with symptomatic oral and genital herpes disease.
  
  
  METHODS
  APOE was genotyped in 182 subjects with herpes simplex virus (HSV) 2 and in 62 subjects with HSV-1, including 44 subjects with both viral types for a total of 200 adults. HSV shedding was measured by PCR from swab samples obtained daily from mucosa for at least 30 days. Participants also maintained a diary of oral or genital lesions.
  
  
  RESULTS
  The APOE genotypes observed reflected the US white population and the Hardy-Weinberg equilibrium. Genital and oral HSV shedding was detected on 17.2% and 3.7% of overall days, respectively, whereas genital and oral lesion rates were 10.1% and 2.9%. Using Poisson regression and adjusting for known correlates of HSV shedding, a significant association was not observed between the APOE genotype and genital or oral HSV shedding, or genital HSV lesions. However, the presence of the APOE4 allele was associated with a higher rate of oral herpetic lesions, with a relative risk of 4.64 (95% CI 1.32 to 15.05, p=0.016).
  
  
  CONCLUSIONS
  Variation at the APOE locus may be associated with clinical manifestations of HSV-1 infection, but does not appear to correlate with herpes simplex viral reactivation in humans.
authors.affiliation: Department of Medicine, University of Washington, Seattle, Washington 98102, USA. viralimm@u.washington.edu
authors.forename: David M
authors.initials: DM
authors.lastname: Koelle
authors.forename: Amalia
authors.initials: A
authors.lastname: Magaret
authors.forename: Terri
authors.initials: T
authors.lastname: Warren
authors.forename: Gerard D
authors.initials: GD
authors.lastname: Schellenberg
authors.forename: Anna
authors.initials: A
authors.lastname: Wald
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D001057:Apolipoproteins E / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006558:Herpes Genitalis / Q000235:genetics* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000502:physiology; D018258:Herpesvirus 2, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011379:Prognosis; D013283:Stomatitis, Herpetic / Q000235:genetics* / Q000821:virology; D051195:Toll-Like Receptor 2 / Q000235:genetics; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D001057:Apolipoproteins E; D004279:DNA, Viral; D051195:Toll-Like Receptor 2
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