title: Association of interleukin-10, interferon-gamma, transforming growth factor-beta, and tumor necrosis factor-alpha gene polymorphisms with long-term kidney allograft survival.
abstract: BACKGROUND
  Single nucleotide polymorphisms within promoter or other regulatory sequences of cytokine genes mainly influence the level of production and secretion of proteins. A large amount of evidence has shown that cytokine gene variations alter graft survival length after kidney transplantation. We studied the association of gene polymorphisms in the interlekin-10 gene (IL10; -1082 G/A), interferon-gamma gene (IFNG; +874 T/A), transforming growth factor-beta gene (TGFB; +869 T/C), and tumor necrosis factor-alpha gene (TNFA; -308 A/G) with kidney allograft survival.
  
  
  METHODS
  The IL10 (-1082 G/A), IFNG (+874 T/A), TGFB (+869 T/C), and TNFA (-308 A/G) genotypes were determined in 32 kidney allograft recipients with graft rejection during the 1st posttransplant year and 52 without rejection in 5 posttransplant years, using allele-specific oligonucleotides-polymerase chain reaction method.
  
  
  RESULTS
  The IFNG +874 A/T genotype showed a significantly higher frequency among kidney recipients of the rejection group than the control group (odds ratio, 2.64, 95% confidence interval, 1.03 to 6.74; P = .04). Comparisons between the rejection and control groups in IL10 (-1082 G/A), IFNG (+874 T/A), TGFB (+869 T/C), and TNFA (-308 A/G) single nucleotide polymorphisms showed no significant difference.
  
  
  CONCLUSIONS
  Based on the finding of this study, it seems polymorphisms in the genes that regulate IL-10, IFN-gamma, TGF-beta, and TNF-alpha cytokines do not play a major role in kidney allograft survival, and other potential factors in this regard should be considered.
authors.affiliation: Deptartment of Genetics, Urmia University of Medical Sciences, Urmia, Iran. davood_omrani@yahoo.co.uk
authors.forename: Mir Davood
authors.initials: MD
authors.lastname: Omrani
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Mokhtari
authors.forename: Morteza
authors.initials: M
authors.lastname: Bagheri
authors.forename: Pedram
authors.initials: P
authors.lastname: Ahmadpoor
mesh_terms: D006084:Graft Rejection / Q000235:genetics*; D006085:Graft Survival / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics; D016030:Kidney Transplantation / Q000009:adverse effects*; D020641:Polymorphism, Single Nucleotide; D016212:Transforming Growth Factor beta / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: C508609:IL10 protein, human; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma