Color and monochrome lensless on-chip imaging of Caenorhabditis elegans over a wide field-of-view.
We demonstrate color and monochrome on-chip imaging of Caenorhabditis elegans samples over a wide field-of-view using incoherent lensless in-line holography. Digital reconstruction of the recorded lensless holograms rapidly creates the C. elegans images within <1 s over a field-of-view of >24 mm2. By digitally combining the reconstructed images at three different wavelengths (red, green and blue), color images of dyed samples are also acquired. This wide field-of-view and compact on-chip imaging modality also permits straightforward integration with microfluidic systems.
Isikman
Serhan O
SO
Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.
Sencan
Ikbal
I
Mudanyali
Onur
O
Bishara
Waheb
W
Oztoprak
Cetin
C
Ozcan
Aydogan
A
D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology*; D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006696:Holography / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*
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