Yuhushiella deserti gen. nov., sp. nov., a new member of the suborder Pseudonocardineae.
A thermotolerant, Gram-stain-positive, aerobic, sporangium-forming actinomycete, strain RA45(T), was isolated from a desert region in Xinjiang Uigur Autonomous Region, north-western China. Comparative analysis of the 16S rRNA gene sequence and phenotypic characterization revealed that strain RA45(T) belonged phylogenetically to the family Pseudonocardiaceae of the suborder Pseudonocardineae. Strain RA45(T) showed more than 5  % 16S rRNA gene sequence divergence from recognized species of genera in the family Pseudonocardiaceae, forming a distinct lineage within the evolutionary radiation occupied by the genera Amycolatopsis, Prauserella, Thermocrispum, Saccharomonospora, Saccharopolyspora and Sciscionella, but distinct from each of them. The affiliation to the family was supported by the presence of suborder- and family-specific 16S rRNA signature nucleotides, a DNA G+C content of 69.9 mol%, the presence of meso-diaminopimelic acid, ribose, arabinose, glucose and galactose, which are characteristic components of cell-wall chemotype IV of actinomycetes, the presence of menaquinone MK-9(H₄) as the major respiratory lipoquinone, a lack of mycolic acids and the presence of an N-acetylated type of muramic acid. However, strain RA45(T) differed from known genera of the family in its polar lipid composition: the major phospholipids were phosphatidylethanolamine, phosphatidylinositol mannosides, phosphatidylmethylethanolamine, diphosphatidylglycerol, phospholipids of unknown structure and phospholipids of unknown structure containing glucosamine (phospholipid type IV). Based on its morphological, chemotaxonomic and phylogenetic characteristics, strain RA45(T) is considered to represent a novel species of a new genus in the family Pseudonocardiaceae, for which the name Yuhushiella deserti gen. nov., sp. nov. is proposed. The type strain of Yuhushiella deserti is RA45(T) (=CGMCC 4.5579(T) =JCM 16584(T)).
Mao
Jun
J
Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
Wang
Jian
J
Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.|Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
Dai
Huan-Qin
HQ
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
Zhang
Zhi-Dong
ZD
Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
Tang
Qi-Yong
QY
Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
Ren
Biao
B
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
Yang
Na
N
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
Goodfellow
Michael
M
School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
Zhang
Li-Xin
LX
SynerZ Pharmaceuticals Inc., Lexington, MA 02421, USA.|Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
Liu
Zhi-Heng
ZH
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D008969:Molecular Sequence Data; D009112:Muramic Acids / Q000032:analysis; D009171:Mycolic Acids / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009112:Muramic Acids; D009171:Mycolic Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid