title: Cytoskeletal dynamics and spermatogenesis.
abstract: Different cellular events occur during spermatogenesis, and these include (i) mitosis for self-renewal of spermatogonia, (ii) differentiation of type A spermatogonia into type B and commitment of type B spermatogonia to develop into preleptotene primary spermatocytes, (iii) transit of preleptotene/leptotene spermatocytes across the blood-testis barrier in coordination with germ cell cycle progression and meiosis, (iv) spermiogenesis and spermiation. These events also associate with extensive changes in cell shape and size, and germ cell movement. The cytoskeleton, which comprises actin, microtubules and intermediate filaments, is believed to function in these cellular events. However, few studies have been conducted by investigators in the past decades to unfold the role of the cytoskeleton during spermatogenesis. This review summarizes recent advances in the field relating to cytoskeletal dynamics in the testis, and highlights areas of research that require additional emphasis so that new approaches for male contraception, as well as therapeutic approaches to alleviate environmental toxicant-induced reproductive dysfunction in men, can possibly be developed.
authors.affiliation: The Mary M. Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA.
authors.forename: Pearl P Y
authors.initials: PP
authors.lastname: Lie
authors.forename: Dolores D
authors.initials: DD
authors.lastname: Mruk
authors.forename: Will M
authors.initials: WM
authors.lastname: Lee
authors.forename: C Yan
authors.initials: CY
authors.lastname: Cheng
mesh_terms: D002450:Cell Communication / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000502:physiology; D003599:Cytoskeleton / Q000502:physiology*; D006801:Humans; D008297:Male; D012670:Seminiferous Epithelium / Q000166:cytology / Q000502:physiology; D012708:Sertoli Cells / Q000166:cytology / Q000502:physiology; D013091:Spermatogenesis / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology
chemical_list: D003598:Cytoskeletal Proteins
references.citation: Endocrinology. 2009 Oct;150(10):4724-33
references.pmid: 19574397
references.citation: FEBS Lett. 2008 Sep 3;582(20):2998-3004
references.pmid: 18692047
references.citation: J Cell Biol. 1999 Sep 6;146(5):1033-44
references.pmid: 10477757
references.citation: J Cell Sci. 2006 Oct 15;119(Pt 20):4155-63
references.pmid: 17038542
references.citation: FASEB J. 2009 Aug;23(8):2555-67
references.pmid: 19293393
references.citation: Toxicol Sci. 2002 Sep;69(1):175-82
references.pmid: 12215672
references.citation: Nat Cell Biol. 2005 Jun;7(6):581-90
references.pmid: 15895076
references.citation: J Cell Sci. 2008 Jan 15;121(Pt 2):196-204
references.pmid: 18187451
references.citation: Arch Histol Cytol. 2000 Mar;63(1):1-15
references.pmid: 10770585
references.citation: Tissue Cell. 1991;23(2):235-46
references.pmid: 1853336
references.citation: Cell Mol Life Sci. 2006 Oct;63(19-20):2329-41
references.pmid: 16909209
references.citation: Tissue Cell. 1989;21(3):361-79
references.pmid: 2479117
references.citation: J Cell Sci. 2000 Jun;113 ( Pt 12):2167-76
references.pmid: 10825290
references.citation: EMBO J. 2000 Nov 15;19(22):6020-9
references.pmid: 11080149
references.citation: Dev Dyn. 2008 Aug;237(8):2170-6
references.pmid: 18651670
references.citation: Dev Biol. 2002 Jan 15;241(2):259-72
references.pmid: 11784110
references.citation: Nat Rev Mol Cell Biol. 2004 Aug;5(8):601-13
references.pmid: 15366704
references.citation: J Cell Sci. 1992 Jul;102 ( Pt 3):401-16
references.pmid: 1506423
references.citation: Dev Biol. 2009 Mar 1;327(1):48-61
references.pmid: 19103189
references.citation: J Cell Biol. 1984 Aug;99(2):529-35
references.pmid: 6746738
references.citation: Endocrinology. 2003 May;144(5):2141-63
references.pmid: 12697723
references.citation: J Biol Chem. 1997 Mar 21;272(12):7841-5
references.pmid: 9065449
references.citation: J Cell Biol. 2009 Jun 15;185(6):939-48
references.pmid: 19528294
references.citation: Traffic. 2004 Jul;5(7):470-7
references.pmid: 15180824
references.citation: Biol Reprod. 1999 Apr;60(4):1036-46
references.pmid: 10084982
references.citation: Mol Biol Cell. 1997 Jun;8(6):973-85
references.pmid: 9201709
references.citation: Biol Reprod. 2004 Mar;70(3):805-12
references.pmid: 14627549
references.citation: Nat Mater. 2006 Sep;5(9):748-53
references.pmid: 16921360
references.citation: Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10166-10173
references.pmid: 16801540
references.citation: J Cell Sci. 1989 Jun;93 ( Pt 2):309-23
references.pmid: 2515196
references.citation: J Biol Chem. 2001 Aug 17;276(33):31449-58
references.pmid: 11418599
references.citation: J Cell Biol. 2001 Feb 5;152(3):471-82
references.pmid: 11157975
references.citation: Exp Cell Res. 2002 Apr 15;275(1):92-109
references.pmid: 11925108
references.citation: Contraception. 2005 Oct;72(4):251-61
references.pmid: 16181968
references.citation: J Androl. 2001 Nov-Dec;22(6):1030-52
references.pmid: 11700851
references.citation: Curr Opin Cell Biol. 2003 Feb;15(1):23-30
references.pmid: 12517700
references.citation: Biol Reprod. 1988 Aug;39(1):105-18
references.pmid: 3207792
references.citation: Curr Biol. 2003 Aug 5;13(15):R614-24
references.pmid: 12906817
references.citation: J Cell Sci. 1996 Jun;109 ( Pt 6):1229-39
references.pmid: 8799813
references.citation: Sci Signal. 2009 Aug 11;2(83):pe49
references.pmid: 19671926
references.citation: Anat Rec. 1988 Feb;220(2):143-60
references.pmid: 3281507
references.citation: J Biol Chem. 2004 Aug 20;279(34):35557-63
references.pmid: 15205456
references.citation: Nat Cell Biol. 2004 Dec;6(12):1180-8
references.pmid: 15558031
references.citation: J Androl. 2004 Mar-Apr;25(2):200-15
references.pmid: 14760006
references.citation: Toxicol Appl Pharmacol. 1987 May;88(3):383-96
references.pmid: 3576623
references.citation: FASEB J. 2008 Jun;22(6):1945-59
references.pmid: 18192323
references.citation: Eur J Cell Biol. 1991 Apr;54(2):277-90
references.pmid: 1879439
references.citation: Biol Reprod. 1993 Jan;48(1):143-53
references.pmid: 8418902
references.citation: J Cell Biol. 1985 Mar;100(3):814-25
references.pmid: 3882723
references.citation: J Biochem. 1998 Apr;123(4):571-8
references.pmid: 9538245
references.citation: J Biol Chem. 1994 Nov 25;269(47):29710-4
references.pmid: 7961961
references.citation: J Cell Sci. 2007 Oct 15;120(Pt 20):3491-9
references.pmid: 17928305
references.citation: Mol Biol Cell. 1999 Dec;10(12):4327-39
references.pmid: 10588661
references.citation: Anat Rec (Hoboken). 2007 Mar;290(3):324-9
references.pmid: 17525947
references.citation: Nat Rev Mol Cell Biol. 2009 Jan;10(1):21-33
references.pmid: 19197329
references.citation: Biol Reprod. 2009 Jan;80(1):153-61
references.pmid: 18799755
references.citation: J Cell Biol. 2001 Sep 3;154(5):1031-44
references.pmid: 11524436
references.citation: Nat Cell Biol. 2004 Feb;6(2):154-61
references.pmid: 14743221
references.citation: Biochim Biophys Acta. 2010 Feb;1803(2):164-73
references.pmid: 19631698
references.citation: Exp Cell Res. 2002 Oct 1;279(2):177-87
references.pmid: 12243744
references.citation: J Biol Chem. 1994 May 27;269(21):14869-71
references.pmid: 8195116
references.citation: Curr Opin Cell Biol. 2006 Oct;18(5):541-8
references.pmid: 16905303
references.citation: Endocr Rev. 2004 Oct;25(5):747-806
references.pmid: 15466940
references.citation: Biol Reprod. 2003 Aug;69(2):475-82
references.pmid: 12672658
references.citation: Nat Struct Mol Biol. 2005 Jan;12(1):26-31
references.pmid: 15592479
references.citation: Nat Rev Mol Cell Biol. 2008 Nov;9(11):860-73
references.pmid: 18946475
references.citation: Exp Cell Res. 2008 Jan 1;314(1):213-26
references.pmid: 17964570
references.citation: Annu Rev Cell Dev Biol. 2005;21:247-69
references.pmid: 16212495
references.citation: Biol Reprod. 2007 Dec;77(6):1037-48
references.pmid: 17855729
references.citation: Biochim Biophys Acta. 2003 Feb 17;1593(2-3):121-9
references.pmid: 12581856
references.citation: Cell. 2004 Jan 23;116(2):167-79
references.pmid: 14744429
references.citation: J Biol Chem. 2006 Jun 23;281(25):17286-17303
references.pmid: 16608848
references.citation: Biol Reprod. 2003 Jun;68(6):2189-206
references.pmid: 12606349
references.citation: Cell Motil Cytoskeleton. 1997;37(2):127-38
references.pmid: 9186010
references.citation: J Biol Chem. 1979 Oct 25;254(20):9982-5
references.pmid: 489616
references.citation: Anat Rec. 1979 Jun;194(2):213-32
references.pmid: 464323
references.citation: Cell Motil Cytoskeleton. 2002 Mar;51(3):147-64
references.pmid: 11921171
references.citation: Dev Biol. 2006 Mar 1;291(1):83-95
references.pmid: 16413524
references.citation: J Cell Biol. 1998 Oct 5;143(1):121-33
references.pmid: 9763425
references.citation: Cell. 2008 Nov 28;135(5):948-59
references.pmid: 19041755
references.citation: Oncogene. 2002 Aug 29;21(38):5967-74
references.pmid: 12185600
references.citation: Nature. 2004 Mar 11;428(6979):198-202
references.pmid: 15014504
references.citation: Nat Cell Biol. 2006 Dec;8(12):1337-47
references.pmid: 17115031
references.citation: Curr Opin Cell Biol. 2008 Feb;20(1):85-90
references.pmid: 18083360
references.citation: Annu Rev Cell Dev Biol. 1997;13:83-117
references.pmid: 9442869
references.citation: J Cell Biol. 2004 Sep 27;166(7):1003-14
references.pmid: 15381688
references.citation: Trends Endocrinol Metab. 2004 Nov;15(9):439-47
references.pmid: 15519891
references.citation: Nature. 2008 Jun 5;453(7196):751-6
references.pmid: 18480755
references.citation: Nat Cell Biol. 2000 Jun;2(6):376-8
references.pmid: 10854330
references.citation: Nat Struct Mol Biol. 2004 Aug;11(8):747-55
references.pmid: 15235593
references.citation: Endocrinology. 2009 Apr;150(4):1861-9
references.pmid: 19095743
references.citation: Nat Cell Biol. 2007 Oct;9(10):1110-21
references.pmid: 17909522
references.citation: Biol Reprod. 2004 Aug;71(2):548-59
references.pmid: 15084482
references.citation: J Endocrinol. 2006 Dec;191(3):571-86
references.pmid: 17170215
references.citation: Mol Hum Reprod. 2005 Jan;11(1):53-64
references.pmid: 15591451
references.citation: Fundam Appl Toxicol. 1995 Feb;24(2):173-82
references.pmid: 7737429
references.citation: Curr Opin Cell Biol. 2009 Feb;21(1):68-73
references.pmid: 19185482
references.citation: Nat Rev Mol Cell Biol. 2004 Aug;5(8):635-46
references.pmid: 15366707
references.citation: Nat Rev Cancer. 2004 Apr;4(4):253-65
references.pmid: 15057285
references.citation: Trends Cell Biol. 2006 Mar;16(3):135-43
references.pmid: 16469495
references.citation: J Endocrinol. 2006 Sep;190(3):759-70
references.pmid: 17003277
references.citation: J Cell Physiol. 2007 Mar;210(3):644-54
references.pmid: 17133358
references.citation: Genes Dev. 2005 Oct 15;19(20):2501-15
references.pmid: 16230537
references.citation: Hum Reprod. 2004 Nov;19(11):2594-604
references.pmid: 15471936
references.citation: Bioessays. 2007 Jan;29(1):36-48
references.pmid: 17187371
references.citation: J Cell Biol. 2000 Jul 24;150(2):361-76
references.pmid: 10908578