title: Anaplasma phagocytophilum from Rodents and Sheep, China.
abstract: To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China. We isolated 3 strains (2 from rodents and 1 from sick sheep) through propagation in BALB/c mice and then cell culture in HL60 cells. The 3 isolates were identified by Wright-Giemsa staining, immunofluorescence, and electronic microscopy and were characterized by sequence analyses of the 16S rRNA gene, partial citrate synthase gene, major surface protein 4 gene, and heat shock protein gene. The multiple sequences of the 3 isolates were identical to each other but different from all known strains from other countries. The public health and veterinary relevance of the isolates deserves further investigation.
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, Beijing, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhan
authors.forename: Wu Chun
authors.initials: WC
authors.lastname: Cao
authors.forename: Jia Fu
authors.initials: JF
authors.lastname: Jiang
authors.forename: Xiao Ai
authors.initials: XA
authors.lastname: Zhang
authors.forename: Yun Xi
authors.initials: YX
authors.lastname: Liu
authors.forename: Xiao Ming
authors.initials: XM
authors.lastname: Wu
authors.forename: Wen Yi
authors.initials: WY
authors.lastname: Zhang
authors.forename: Pan He
authors.initials: PH
authors.lastname: Zhang
authors.forename: Chang Ling
authors.initials: CL
authors.lastname: Bian
authors.forename: J Stephen
authors.initials: JS
authors.lastname: Dumler
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Shu Qing
authors.initials: SQ
authors.lastname: Zuo
authors.forename: Chen Yi
authors.initials: CY
authors.lastname: Chu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jan H
authors.initials: JH
authors.lastname: Richardus
authors.forename: J Dik F
authors.initials: JD
authors.lastname: Habbema
mesh_terms: D041081:Anaplasma phagocytophilum / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D002681:China / Q000453:epidemiology; D002950:Citrate (si)-Synthase / Q000032:analysis / Q000235:genetics; D006224:Cricetinae; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016873:Ehrlichiosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D018922:HL-60 Cells; D006360:Heat-Shock Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012376:Rodent Diseases / Q000453:epidemiology / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D012336:RNA, Ribosomal, 16S; D002950:Citrate (si)-Synthase
references.citation: Appl Environ Microbiol. 2006 Feb;72(2):1102-9
references.pmid: 16461655
references.citation: J Clin Microbiol. 2005 Mar;43(3):1309-17
references.pmid: 15750101
references.citation: Vet Res Commun. 2007 Aug;31 Suppl 1:79-84
references.pmid: 17682851
references.citation: Brief Bioinform. 2004 Jun;5(2):150-63
references.pmid: 15260895
references.citation: Int J Syst Evol Microbiol. 2001 Nov;51(Pt 6):2145-2165
references.pmid: 11760958
references.citation: J Infect Dis. 2003 Dec 1;188(11):1757-63
references.pmid: 14639548
references.citation: Ann N Y Acad Sci. 2003 Jun;990:118-25
references.pmid: 12860612
references.citation: Vector Borne Zoonotic Dis. 2008 Jun;8(3):369-80
references.pmid: 18471056
references.citation: Clin Infect Dis. 2007 Jul 15;45 Suppl 1:S45-51
references.pmid: 17582569
references.citation: Vector Borne Zoonotic Dis. 2008 Spring;8(1):49-55
references.pmid: 18047398
references.citation: JAMA. 2008 Nov 19;300(19):2263-70
references.pmid: 19017912
references.citation: Vector Borne Zoonotic Dis. 2006 Spring;6(1):83-90
references.pmid: 16584330
references.citation: N Engl J Med. 1996 Jan 25;334(4):209-15
references.pmid: 8531996
references.citation: J Clin Microbiol. 1995 Jul;33(7):1704-11
references.pmid: 7665632
references.citation: Am J Trop Med Hyg. 2003 May;68(5):547-50
references.pmid: 12812342
references.citation: Ann N Y Acad Sci. 2006 Oct;1078:106-9
references.pmid: 17114688
references.citation: Am J Trop Med Hyg. 2006 Oct;75(4):664-8
references.pmid: 17038691
references.citation: Infect Immun. 2005 Jun;73(6):3440-4
references.pmid: 15908372
references.citation: Emerg Infect Dis. 2005 Nov;11(11):1780-3
references.pmid: 16318739
references.citation: Ann N Y Acad Sci. 2008 Dec;1149:94-7
references.pmid: 19120182
references.citation: Clin Vaccine Immunol. 2008 Mar;15(3):418-24
references.pmid: 18094110
references.citation: J Clin Microbiol. 2001 Jan;39(1):335-8
references.pmid: 11136794
references.citation: Clin Infect Dis. 2003 Sep 15;37(6):846-8
references.pmid: 12955649