title: Yaniella fodinae sp. nov., isolated from a coal mine.
abstract: An orange bacterial strain, designated G5(T), was isolated during the study of the bacterial diversity of a coal mine. The cell wall of strain G5(T) contained peptidoglycan type A4α (l-Lys-Gly-l-Glu) and the sugars xylose and mannose. The major menaquinones were MK-8 (45.0 %) and MK-9 (34.0 %) and minor amounts of MK-7 and MK-8(H(2)) were also found. The major fatty acids were anteiso-C(15 : 0) (44.9 %) and iso-C(15 : 0) (44.2 %). The main cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. 16S rRNA gene sequence analysis showed that strain G5(T) was closely related to Yaniella halotolerans YIM 70085(T) and Yaniella flava YIM 70178(T) (both 96.7 % 16S rRNA gene sequence similarity). The genomic DNA G+C content of strain G5(T) was 61.6&emsp14;mol%. These data and other phenotypic characteristics clearly indicated that strain G5(T) represents a novel species of the genus Yaniella, for which the name Yaniella fodinae sp. nov. is proposed. The type strain is G5(T) (=MTCC 9846(T)=DSM 22966(T)).
authors.affiliation: Division of Environmental Biotechnology and Microbial Biochemistry, Institute of Microbial Technology, Chandigarh 160 036, India.
authors.forename: Soniya
authors.initials: S
authors.lastname: Dhanjal
authors.affiliation: MTCC-Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology, Chandigarh 160 036, India.
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Ruckmani
authors.affiliation: Division of Environmental Biotechnology and Microbial Biochemistry, Institute of Microbial Technology, Chandigarh 160 036, India.
authors.forename: Swaranjit Singh
authors.initials: SS
authors.lastname: Cameotra
authors.affiliation: DSMZ-German Collection of Microorganisms and Cell Cultures, 38124, Braunschweig, Germany.
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Pukall
authors.affiliation: DSMZ-German Collection of Microorganisms and Cell Cultures, 38124, Braunschweig, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: MTCC-Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology, Chandigarh 160 036, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Mayilraj
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000302:isolation & purification*; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D003031:Coal; D003593:Cytoplasm / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D002241:Carbohydrates; D003031:Coal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2