[(Methyl)1-(11)c]-acetate metabolism in hepatocellular carcinoma.
OBJECTIVE
Studies have established the value of [(methyl)1-(11)C]-acetate ([(11)C]Act) combined with 2-deoxy-2[(18)F]fluoro-D-glucose (FDG) for detecting hepatocellular carcinoma (HCC) using positron emission tomography (PET). In this study, the metabolic fate of [(11)C]Act in HCC was characterized.


METHODS
Experiments with acetic acid [1-(14)C] sodium salt ([(14)C]Act) were carried out on WCH-17 cells and freshly derived rat hepatocytes. PET scans with [(11)C]Act were also carried out on woodchucks with HCC before injection of [(14)C]Act. The radioactivity levels in different metabolites were quantified with thin-layer chromatography.


RESULTS
In WCH-17 cells, the predominant metabolite was phosphatidylcholine (PC). Regions of HCCs with the highest [(11)C]Act uptake had higher radioactivity accumulation in lipid-soluble compounds than surrounding hepatic tissues. In those regions, PC and triacylglycerol (TG) accumulated more radioactivity than in surrounding hepatic tissues.


CONCLUSIONS
High [(11)C]Act uptake in HCC is associated with increased de novo lipogenesis. PC and TG are the main metabolites into which the radioactive label from [(11)C]Act is incorporated in HCC.
Salem
Nicolas
N
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA.
Kuang
Yu
Y
Corn
David
D
Erokwu
Bernadette
B
Kolthammer
Jeffrey A
JA
Tian
Haibin
H
Wu
Chunying
C
Wang
Fangjing
F
Wang
Yanming
Y
Lee
Zhenghong
Z
D000085:Acetates / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes / Q000378:metabolism*; D045744:Cell Line, Tumor; D022781:Hepatocytes / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D049268:Positron-Emission Tomography; D051381:Rats
D000085:Acetates; D002250:Carbon Radioisotopes; C046923:methyl acetate
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