Molecular mechanisms of enhanced wound healing by copper oxide-impregnated dressings.
ABSTRACT Copper plays a key role in angiogenesis and in the synthesis and stabilization of extracellular matrix skin proteins, which are critical processes of skin formation. We hypothesized that introducing copper into wound dressings would enhance wound repair. Application of wound dressings containing copper oxide to wounds inflicted in genetically engineered diabetic mice (C57BL/KsOlaHsd-Lepr(db)) resulted in increased gene and in situ up-regulation of proangiogenic factors (e.g., placental growth factor, hypoxia-inducible factor-1 alpha, and vascular endothelial growth factor), increased blood vessel formation (p<0.05), and enhanced wound closure (p<0.01) as compared with control dressings (without copper) or commercial wound dressings containing silver. This study proves the capacity of copper oxide-containing wound dressings to enhance wound healing and sheds light onto the molecular mechanisms by which copper oxide-impregnated dressings stimulate wound healing.
Borkow
Gadi
G
Cupron Scientific, Modi'in, Israel. gadi@cupron.com
Gabbay
Jeffrey
J
Dardik
Rima
R
Eidelman
Arthur I
AI
Lavie
Yossi
Y
Grunfeld
Yona
Y
Ikher
Sergey
S
Huszar
Monica
M
Zatcoff
Richard C
RC
Marikovsky
Moshe
M
D000818:Animals; D030801:Animals, Genetically Modified; D001458:Bandages; D003300:Copper / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic; D000072483:Placenta Growth Factor; D011257:Pregnancy Proteins / Q000378:metabolism; D012867:Skin / Q000473:pathology*; D014131:Trace Elements / Q000494:pharmacology*; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C000608037:Pgf protein, mouse; D011257:Pregnancy Proteins; D014131:Trace Elements; D042461:Vascular Endothelial Growth Factor A; D000072483:Placenta Growth Factor; D003300:Copper; C000520:cuprous oxide