title: [Expression of bursin receptor on membranes of hybridoma cell and identification of binding peptide of bursin from 12-merrandom phage display peptide library].
abstract: OBJECTIVE
  Bursin (BS) could greatly promote the production of monoclonal antibody in hybridoma cell. We studied the interaction between BS and hybridoma cell.
  
  
  METHODS
  Fluorescence microscopy, Laser Scanning Confocal Microscope (LSCM) and fluorescence activated cell sorter (FACS) were used.
  
  
  RESULTS
  Fluorescence microscopy revealed specific binding of FITC-BS to hybridoma cell. FACS analysis demonstrated that the binding was specific, saturable and reversibility. BS was used as target protein to screen its binding peptides from 12-mer random phage display peptide library. After four rounds of biopanning, 20 phage clones were randomly selected and identified. ELISA and competitive inhibition test results indicated that 2 phage clones were identified as positive clones. The amino acid sequences analysis shown that the sequences were ACTKHLCLLQPL or MSCNDTLCLLPN, which sharing a conservative sequence LCLL. In vitro experiments suggested that the two binding peptides can inhibit BS specific binding to hybridoma cell.
  
  
  CONCLUSIONS
  These results confirmed that existence of BS receptor in the membrane of hybridoma cell, which involved in hybridoma cell secreting monoclonal antibody signal pathway.
authors.affiliation: Lab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China. wangchen2001@126.com
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Zhanqin
authors.initials: Z
authors.lastname: Zhao
authors.forename: Mingfu
authors.initials: M
authors.lastname: Niu
authors.forename: Chunjie
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiangchao
authors.initials: X
authors.lastname: Cheng
authors.forename: Puyan
authors.initials: P
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D015870:Gene Expression; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism*; D019151:Peptide Library; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*
chemical_list: D009842:Oligopeptides; D019151:Peptide Library; D011956:Receptors, Cell Surface; C016281:bursopoietin