title: Induced pluripotent stem cells: what lies beyond the paradigm shift.
abstract: The discovery that somatic cells can be reprogrammed to become induced pluripotent stem (iPS) cells has ushered in a new and exciting era in regenerative medicine. Since the seminal discovery of somatic cell reprogramming by Takahashi and Yamanaka in 2006, there has been remarkable progress in the characterization of iPS cells and the protocols used to generate them. The new information generated during the past year alone has vastly expanded our understanding of these cells. Accordingly, this review provides a basic overview of the different strategies used to generate iPS cells and focuses on recent developments in the field of iPS cells. In the final section, we discuss three broad, unanswered questions related to somatic cell reprogramming, which are just starting to be addressed.
authors.affiliation: Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198-6805, USA.
authors.forename: Jesse L
authors.initials: JL
authors.lastname: Cox
authors.forename: Angie
authors.initials: A
authors.lastname: Rizzino
mesh_terms: D000818:Animals; D065150:Cellular Reprogramming / Q000235:genetics*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D044127:Epigenesis, Genetic; D005260:Female; D020869:Gene Expression Profiling; D016158:Genes, p53; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D016662:Virus Integration / Q000235:genetics; D049951:X Chromosome Inactivation / Q000235:genetics
chemical_list: D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse
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