Measurement of ascorbic acid in single rat peritoneal mast cells using capillary electrophoresis with electrochemical detection.
In this paper, the amount of ascorbic acid (AA) in single rat peritoneal mast cell was determined by the method of capillary electrophoresis (CE) with electrochemical detection (ED) at a carbon fiber microdisk bundle electrode. The CE-ED system and the single-cell injection system were rearranged to make the operation more convenient and efficient. In the experiment, a self-made holder made of foam was used to keep the capillary from swing, which kept the stability of the baseline of the electropherogram. The single cell was lysed completely within 5s using the 0.1% sodium dodecylsulfate (SDS) as the cell lysis solution together with the lysis voltage of 2 kV. The quantitation analysis was accomplished by the use of calibration curves, and the amount of AA in single rat peritoneal mast cell was from 2.4 to 7.1 fmol.
He
Peng
P
Key Laboratory of Eco-chemical Engineering Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Zhengzhou Road 53, Sifang, Qingdao 266042, China.
Niu
Yan
Y
Mei
Zhen-Hua
ZH
Bao
Jun-Fang
JF
Sun
Xue-Mei
XM
D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis*; D002457:Cell Extracts / Q000737:chemistry; D002469:Cell Separation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D008297:Male; D008407:Mast Cells / Q000737:chemistry*; D010537:Peritoneum / Q000166:cytology*; D051381:Rats; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
D002457:Cell Extracts; D012967:Sodium Dodecyl Sulfate; D001205:Ascorbic Acid