Combined drug medium with isoniazid and rifampicin for identification of multi-drug resistant Mycobacterium tuberculosis.
A low-cost method of detecting multi-drug resistant Mycobacterium tuberculosis (MDR-TB) with the possibility of quick adoption in a resource limited setting is urgently required. We conducted a study combining isoniazid and rifampicin in a single LJ medium, to detect MDR-TB strains. Combined and individual drug media showed 100% concordance for the detection of MDR-TB and susceptible strains by proportion method. Considering the results, combined isoniazid and rifampicin containing medium could be considered for use in settings where the sole detection of MDR-TB strains is justified.
Nalini
S
S
Department of Bacteriology, Tuberculosis Research Centre, (ICMR) Chennai - 600 031, India.
Lakshmi
R
R
Devika
K
K
Ravikumar
D
D
Ramachandran
R
R
D000995:Antitubercular Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000302:isolation & purification*; D012293:Rifampin / Q000494:pharmacology*; D012641:Selection, Genetic; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000382:microbiology
D000995:Antitubercular Agents; D003470:Culture Media; D007538:Isoniazid; D012293:Rifampin