Quantitative microtomography of the early mammalian embryo by laser scanning microscopy.
The blastomer volume was measured by three-dimensional (3D) reconstruction of a series of successive optical sections of the early mouse embryo. Changes in cell volume during osmotic shock were studied. Incubation of a two-cell embryo in Dulbecco's medium induced slow shrinkage of the embryonic cells followed by recovery of its initial volume. A regulatory phase of osmotic shock compensation is characteristic of a blastomer under hypotonic conditions.
Pogorelov
A G
AG
Institute of Theoretical and Experimental Biophysics, the Russian Academy of Sciences, Pushchino, Russia. agpogorelov@rambler.ru
Pogorelova
V N
VN
D000818:Animals; D004622:Embryo, Mammalian / Q000033:anatomy & histology*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018613:Microscopy, Confocal / Q000379:methods*