Investigating Association of Three Polymorphisms of Coagulation Factor XIII and Recurrent Pregnancy Loss.
OBJECTIVE
among important suspected causes of thrombophilia in women with recurrent pregnancy loss (RPL) are the polymorphisms of coagulation factor XIII (FXIII) gene. We performed a case-control study on the association between three polymorphisms of factor XIII (FXIII G103T, FXIII A614T and FXIII C1694T) and RPL in Iranian women.


METHODS
DNA samples from peripheral blood of 100 female patients with at least two recurrent abortions, as case group, and 100 healthy women with history of at least two successful deliveries were subjected to PCR-RFLP, and the frequencies of the polymorphisms were calculated and compared between the two groups.


RESULTS
the prevalence of FXIII G103T polymorphism was 29% in the case group and 17% in the control group (P = 0.158). The frequencies of FXIII A614T and FXIII C1694T were 84% and 66% in the case group and 48% and 31% in the control group (P <0.001 and P < 0.001), respectively. The two latter polymorphisms are associated with RPL in Iranian women and increase the risk of RPL. A correlation was also found between FXIII A614T and FXIII C1694T polymorphisms (P < 0.001).


CONCLUSIONS
we suggest the evaluation of FXIII A614T and FXIII C1694T polymorphisms in women with RPL.
Jeddi-Tehrani
Mahmood
M
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. mahjed@yahoo.com
Torabi
Raheleh
R
Mohammadzadeh
Afsaneh
A
Arefi
Soheila
S
Keramatipour
Mohammad
M
Zeraati
Hojjat
H
Zarnani
Amir Hasan
AH
Akhondi
Mohammad Mehdi
MM
Mahmoudian
Jafar
J
Mahmoudi
Ahmad Reza
AR
Zarei
Saeed
S
D000026:Abortion, Habitual / Q000453:epidemiology / Q000235:genetics* / Q000503:physiopathology; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D005176:Factor XIII / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007492:Iran; D011110:Polymorphism, Genetic; D011247:Pregnancy; D012307:Risk Factors; D019851:Thrombophilia
D005176:Factor XIII