[Determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici by RP-HPLC].
OBJECTIVE
To develop a RP-HPLC method for the determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi indici.


METHODS
An Eclipse XDB-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phases of methanol-0.2% phosphatic acid in a gradient manner. The flow rate was set at 1.0 mL x min(-1). The detection wavelength was 350 nm.


RESULTS
The linear response ranged from 1.02-20.48 mg x L(-1) for quercetin (r = 0.9994, n = 5), 1.03-20.54 mg x L(-1) for luteolin (r = 0.9992, n = 5), 1.12-22.40 mg x L(-1) for apigenin (r = 0.9995, n = 5), 1.01-20.22 mg x L(-1) for acacetin (r = 0.9998, n = 5), respectively. Recoveries were 101.3% with RSD 1.3% for quercetin, 100.62% with RSD 1.4% for luteolin, 98.42% with RSD 1.7% for apigenin and 99.02% with RSD 0.8% for acacetin. A significant difference (alpha = 0.01) among the contents of four flavonoids and total flavonoids was found.


CONCLUSIONS
The method is quick, simple and repeatable for simultaneous determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici.
Shen
Haijin
H
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Guo
Qiaosheng
Q
Fang
Hailing
H
Wang
Yanru
Y
Jin
Miao
M
D047310:Apigenin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D031202:Chrysanthemum / Q000737:chemistry*; D047309:Flavones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis*; D035264:Flowers / Q000737:chemistry; D047311:Luteolin / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis*; D011794:Quercetin / Q000032:analysis
D047309:Flavones; D005419:Flavonoids; D010936:Plant Extracts; D047310:Apigenin; D011794:Quercetin; D047311:Luteolin; C023717:acacetin