Identification of a new adhesin-like protein from Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens.
OBJECTIVE
To identify and characterize a new adhesin-like protein of probiotics that show specific adhesion to human blood group A and B antigens.


RESULTS
Using the BIACORE assay, the adhesion of cell surface components obtained from four lactobacilli strains that adhered to blood group A and B antigens was tested. Their components showed a significant adhesion to A and B antigens when compared to the bovine serum albumin (BSA) control. The 1 mol l(-1) GHCl fraction extracted from Lactobacillus mucosae ME-340 contained a 29-kDa band (Lam29) using SDS-PAGE. The N-terminal amino acid sequence and homology analysis showed that Lam29 was 90% similar to the substrate-binding protein of the ATP-binding cassette (ABC) transporter from Lactobacillus fermentum IFO 3956. The complete nucleotide sequence (858 bp) of Lam29 was determined and encoded a protein of 285 amino acid residues. Phylogenetic analysis and multiple sequence alignments indicated this protein may be related to the cysteine-binding transporter.


CONCLUSIONS
The adhesion of ME-340 strain to blood group A and B antigens was mediated by Lam29 that is a putative component of ABC transporter as an adhesin-like protein.


CONCLUSIONS
Lactobacillus mucosae ME-340 expressing Lam29 may be useful for competitive exclusion of pathogens via blood group antigen receptors in the human gastrointestinal mucosa and in the development of new probiotic foods.
Watanabe
M
M
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan.
Kinoshita
H
H
Nitta
M
M
Yukishita
R
R
Kawai
Y
Y
Kimura
K
K
Taketomo
N
N
Yamazaki
Y
Y
Tateno
Y
Y
Miura
K
K
Horii
A
A
Kitazawa
H
H
Saito
T
T
D000017:ABO Blood-Group System / Q000378:metabolism*; D018829:Adhesins, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007778:Lactobacillus / Q000378:metabolism*; D008969:Molecular Sequence Data; D019936:Probiotics; D016415:Sequence Alignment; D012710:Serum Albumin, Bovine / Q000378:metabolism
D000017:ABO Blood-Group System; D018829:Adhesins, Bacterial; D012710:Serum Albumin, Bovine