title: Screening of epigenetic and genetic disturbances of human chromosome 3 genes in colorectal cancer.
abstract: DNA microarray technology comprising NotI-linking clones was used in a large-scale study of genetic and epigenetic changes in colorectal cancer. Analysis of samples from 24 patients revealed methylation, deletions, and amplifications in 137 of 181 NotI clones. For 27 genes/loci, these changes occurred in more than 30% of the tumor samples, suggesting that these genes are involved in the development of colorectal cancer. An analysis of the methylation status of CpG island of the ITGA9 gene/loci by bisulfite sequencing confirmed the NotI microarray data on the gene/loci methylation in colorectal cancer. Aberrations in 19 genes/loci were unknown previously. Their characterization may help ascertain the mechanisms responsible for colorectal cancer development and identify novel diagnostic and prognostic markers.
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv. g.v.gerashchenko@imbg.org.ua
authors.forename: G V
authors.initials: GV
authors.lastname: Gerashchenko
authors.forename: V V
authors.initials: VV
authors.lastname: Gordiyuk
authors.forename: I Ya
authors.initials: IY
authors.lastname: Skrypkina
authors.forename: S M
authors.initials: SM
authors.lastname: Kvasha
authors.forename: O O
authors.initials: OO
authors.lastname: Kolesnik
authors.forename: D D
authors.initials: DD
authors.lastname: Ugryn
authors.forename: T V
authors.initials: TV
authors.lastname: Pavlova
authors.forename: E R
authors.initials: ER
authors.lastname: Zabarovsky
authors.forename: A V
authors.initials: AV
authors.lastname: Rynditch
authors.forename: V I
authors.initials: VI
authors.lastname: Kashuba
mesh_terms: D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D056426:Genetic Loci / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*