Optimization of data collection taking radiation damage into account.
To take into account the effects of radiation damage, new algorithms for the optimization of data-collection strategies have been implemented in the software package BEST. The intensity variation related to radiation damage is approximated by log-linear functions of resolution and cumulative X-ray dose. Based on an accurate prediction of the basic characteristics of data yet to be collected, BEST establishes objective relationships between the accessible data completeness, resolution and signal-to-noise statistics that can be achieved in an experiment and designs an optimal plan for data collection.
Bourenkov
Gleb P
GP
EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85b, 22607 Hamburg, Germany.
Popov
Alexander N
AN
D000465:Algorithms; D029063:Aquifoliaceae / Q000737:chemistry; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D048013:Acetivibrio thermocellus / Q000201:enzymology; D018360:Crystallography, X-Ray / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D007328:Insulin / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D034741:RNA, Small Interfering / Q000032:analysis / Q000737:chemistry; D017864:Tombusvirus / Q000737:chemistry; D014764:Viral Proteins / Q000032:analysis / Q000737:chemistry; D014965:X-Rays
D007328:Insulin; D008565:Membrane Proteins; C493885:P19 protein, tomato bushy stunt virus; D034741:RNA, Small Interfering; D014764:Viral Proteins; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase
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