A cellulose synthase-like protein is required for osmotic stress tolerance in Arabidopsis.
Osmotic stress imposed by soil salinity and drought stress significantly affects plant growth and development, but osmotic stress sensing and tolerance mechanisms are not well understood. Forward genetic screens using a root-bending assay have previously identified salt overly sensitive (sos) mutants of Arabidopsis that fall into five loci, SOS1 to SOS5. These loci are required for the regulation of ion homeostasis or cell expansion under salt stress, but do not play a major role in plant tolerance to the osmotic stress component of soil salinity or drought. Here we report an additional sos mutant, sos6-1, which defines a locus essential for osmotic stress tolerance. sos6-1 plants are hypersensitive to salt stress and osmotic stress imposed by mannitol or polyethylene glycol in culture media or by water deficit in the soil. SOS6 encodes a cellulose synthase-like protein, AtCSLD5. Only modest differences in cell wall chemical composition could be detected, but we found that sos6-1 mutant plants accumulate high levels of reactive oxygen species (ROS) under osmotic stress and are hypersensitive to the oxidative stress reagent methyl viologen. The results suggest that SOS6/AtCSLD5 is not required for normal plant growth and development but has a critical role in osmotic stress tolerance and this function likely involves its regulation of ROS under stress.
Zhu
Jianhua
J
Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USA. jhzhu@umd.edu
Lee
Byeong-Ha
BH
Dellinger
Mike
M
Cui
Xinping
X
Zhang
Changqing
C
Wu
Shang
S
Nothnagel
Eugene A
EA
Zhu
Jian-Kang
JK
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D008353:Mannitol / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D009995:Osmosis; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological
D029681:Arabidopsis Proteins; D018744:DNA, Plant; D017382:Reactive Oxygen Species; D008353:Mannitol; D011092:Polyethylene Glycols; D012965:Sodium Chloride; C526694:CSLD5 protein, Arabidopsis; D005964:Glucosyltransferases; D010269:Paraquat
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