title: Functional studies on RNA-transfected cell microarrays.
abstract: RNA-transfected cell microarray shows great promise in functional genomics. By printing siRNA complexed with transfection reagent on glass slides, arrays of transfected cells are formed in which the phenotypic consequences of gene suppression can be investigated. Using reporter plasmids with fluorescence intensity as output data, we have developed a strategy for statistical analysis of the intensity data from medium-scale functional studies using data from several experimental replicates.
authors.affiliation: Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
authors.forename: Christina Saeten
authors.initials: CS
authors.lastname: Fjeldbo
authors.forename: Kristine
authors.initials: K
authors.lastname: Misund
authors.forename: Clara-Cecilie
authors.initials: CC
authors.lastname: Günther
authors.forename: Mette
authors.initials: M
authors.lastname: Langaas
authors.forename: Tonje Strømmen
authors.initials: TS
authors.lastname: Steigedal
authors.forename: Liv
authors.initials: L
authors.lastname: Thommesen
authors.forename: Astrid
authors.initials: A
authors.lastname: Laegreid
authors.forename: Torunn
authors.initials: T
authors.lastname: Bruland
mesh_terms: D002460:Cell Line; D006801:Humans; D016014:Linear Models; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D034741:RNA, Small Interfering / Q000378:metabolism*; D012996:Solutions; D013223:Statistics as Topic; D016707:Tissue Fixation; D014162:Transfection / Q000379:methods*
chemical_list: D034741:RNA, Small Interfering; D012996:Solutions