Induction of apoptosis in densovirus infected Aedes aegypti mosquitoes.
The mechanism of death in densovirus infected mosquitoes remains unexplored. This study investigated the cellular consequences of densovirus infection in Aedes aegypti mosquitoes after a second generation challenge with a densovirus isolated from adult Aedes albopictus mosquitoes in Thailand (AThDNV). Specimens were analyzed by TUNEL assay, fluorescent in situ hybridization (FISH) and a calorimic assay to detect activation of caspase 3-like activity. After challenge, moribund mosquitoes showed considerable evidence of TUNEL positive cells. The caspase 3-like activity assay showed that the presence of TUNEL positive cells was associated with increased levels of activated caspase 3-like activity in AThDNV infected mosquitoes.
Roekring
Songsak
S
Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, Thailand.
Smith
Duncan R
DR
D000330:Aedes / Q000821:virology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3; D004266:DNA Virus Infections / Q000473:pathology / Q000635:transmission / Q000662:veterinary*; D004279:DNA, Viral / Q000032:analysis; D019223:Densovirinae / Q000235:genetics / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D017404:In Situ Hybridization, Fluorescence; D020287:In Situ Nick-End Labeling; D019476:Insect Proteins; D007303:Insect Vectors / Q000821:virology*; D007814:Larva / Q000821:virology; D009032:Mosquito Control; D010572:Pest Control, Biological
D004279:DNA, Viral; D019476:Insect Proteins; D053148:Caspase 3