title: New pyrano-pyrone from Goniothalamus tamirensis enhances the proliferation and differentiation of osteoblastic MC3T3-E1 cells.
abstract: The new pyrano-pyrone, (+)-8-epi-9-deoxygoniopypyrone (1) and (+)-9-deoxygoniopypyrone (2) were isolated from a chloroform extract of Goniothalamus tamirensis leaves. Their absolute stereostructures were discussed and confirmed by using infrared (IR), Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), one (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectra, Mosher's method, and comparison with the known compounds leiocapin A (3), deoxygoniopypyrone A (4), and (-)-8-epi-9-deoxygoniopypyrone (5). At concentrations of 2.67 microM, compounds 1 and 2 significantly increased the growth of osteoblastic MC3T3-E1 cells and caused a significant elevation of collagen content, alkaline phosphatase activity, and nodule mineralization in the cells (p<0.05). Our data suggest that the enhancement of osteoblast function by 1 and 2 may result in the prevention of osteoporosis.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, Korea.
authors.forename: Bui Huu
authors.initials: BH
authors.lastname: Tai
authors.forename: Vu Thi
authors.initials: VT
authors.lastname: Huyen
authors.forename: Tran Thu
authors.initials: TT
authors.lastname: Huong
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Nhiem
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Choi
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.forename: Pham Quoc
authors.initials: PQ
authors.lastname: Long
authors.forename: Nguyen Manh
authors.initials: NM
authors.lastname: Cuong
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism; D054335:Goniothalamus / Q000737:chemistry*; D008968:Molecular Conformation; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011753:Pyrones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D011753:Pyrones; D003094:Collagen; D000469:Alkaline Phosphatase