title: W194XProp1 and S156insTProp1, both of which have intact DNA-binding domain, show a different DNA-binding activity to the Prop1-binding element in human Pit-1 gene.
abstract: Prop1 activates POU1F1 (Pit-1) gene expression, which in turn stimulates GH, PRL, TSHbeta and GHRH receptor gene expressions. Therefore the patients with Prop1 mutation show GH, PRL, and TSH deficiency. The mutation of Prop1 is a major abnormality causing combined pituitary hormone deficiency (CPHD). However, DNA-binding and activating functions of mutant Prop1 have not been examined fully because Prop1-binding elements (PBEs) in human POU1F1 gene were not identified until 2008. The aim of this study is to test DNA-binding and transcriptional activities of two mutant Prop1s (W194XProp1 and S156insTProp1, both of them were found in the patients with CPHD) whose mutation is located in putative transactivating domain but not in DNA-binding domain. W194XProp1 showed a marked DNA-binding to PBE as well as a consensus element of paired-like transcription factors (PRDQ9). Activating function for POU1F1 reporter genes expression was lost or decreased in W194XProp1 but still preserved for PRDQ9 reporter gene. S156insTProp1 did not bind PBE but bound PRDQ9. Consistent with the result, S156insTProp1 did not stimulate POU1F1 reporter gene but stimulated PRDQ9 reporter gene. These results support the inference that W194XProp1 is unable to increase POU1F1 gene expression by the defect of transactivating domain and that S156insTProp1 is unable to increase due to the loss of DNA-binding activity. DNA-binding domain that has been assumed is not sufficient to provide full DNA-binding activity of Prop1 and transactivating domain of Prop1 is likely to affect DNA binding to PBE.
authors.affiliation: Department of Biophysics, Kobe University Graduate School of Health Science, 7-10-2 Tomogaoka, Suma-ku, Kobe 654-0142, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Shibahara
authors.forename: Nobuko
authors.initials: N
authors.lastname: Ikeshita
authors.forename: Yuka
authors.initials: Y
authors.lastname: Sugiyama
authors.forename: Keizo
authors.initials: K
authors.lastname: Toda
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamamoto
authors.forename: Elizabeth Henny
authors.initials: EH
authors.lastname: Herningtyas
authors.forename: Taiki
authors.initials: T
authors.lastname: Maki
authors.forename: Eriko
authors.initials: E
authors.lastname: Kubota
authors.forename: Genzo
authors.initials: G
authors.lastname: Iguchi
authors.forename: Keiji
authors.initials: K
authors.lastname: Iida
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Hidesuke
authors.initials: H
authors.lastname: Kaji
authors.forename: Kazuo
authors.initials: K
authors.lastname: Chihara
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Okimura
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D050817:Transcription Factor Pit-1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; C102134:Prophet of Pit-1 protein; D050817:Transcription Factor Pit-1; D004247:DNA