title: The proteome complement of Nicotiana tabacum Bright-Yellow-2 culture cells.
abstract: The Nicotiana tabacum Bright-Yellow-2 (BY2) cell line is one of most commonly used plant suspension cell lines and offers interesting properties, such as fast growth, amenability to genetic transformation, and synchronization of cell division. To build a proteome reference map of BY2 cell proteins, we isolated the soluble proteins from N. tabacum BY2 cells at the end of the exponential growth phase and analyzed them by 2-DE and MALDI TOF-TOF. Of the 1422 spots isolated, 795 were identified with a significant score, corresponding to 532 distinct proteins.
authors.affiliation: Institut des Sciences de la Vie, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Duby
authors.forename: Hervé
authors.initials: H
authors.lastname: Degand
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Faber
authors.forename: Marc
authors.initials: M
authors.lastname: Boutry
mesh_terms: D002460:Cell Line; D010940:Plant Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D010940:Plant Proteins; D020543:Proteome