title: Change profiles in matrix metalloproteinase-2 and -9 in induced endometriosis in mice.
abstract: To examine the changes in matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) in the development and progression of endometriosis, real time quantitative polymerase chain reaction, enzyme-linked immunoabsorbent assay and gelatin zymography were employed to determine the mRNA and protein levels and activities of MMP-2 and MMP-9 from the first day to the 21(st) day after the induction in mice with induced endometriosis (experimental group) and sham-operated animals (controls). The results showed that the mRNA and protein levels and activities of the MMP-2 and MMP-9 were significantly increased on the first day after the induction and the level of MMP-2 stayed at a level higher than that in the control group. MMP-9 had two or three peaks during the 21 days, taking place at day 1, 4 and 15. It is concluded that the changes in the MMP-2 and MMP-9 might be involved in pathogenesis of endometriosis.
authors.affiliation: Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. cqhua@126.com
authors.forename: Qionghua
authors.initials: Q
authors.lastname: Chen
authors.forename: Naxuan
authors.initials: N
authors.lastname: Qiu
authors.forename: Demin
authors.initials: D
authors.lastname: Pu
authors.forename: Yumin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Tian
authors.initials: T
authors.lastname: Li
authors.forename: Hongyi
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D004715:Endometriosis / Q000201:enzymology* / Q000209:etiology; D005260:Female; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010532:Peritoneal Diseases / Q000201:enzymology* / Q000209:etiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse
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