No juvenile arterial hypertension in sheep multiples despite reduced nephron numbers.
Low birth weight is associated with an increased risk of metabolic dysfunction and arterial hypertension in later life. Because of their reduced birth weight twins have been used repeatedly as a natural model to investigate prenatal programming of hypertension. To reveal an early impact of lower nephron endowment on blood pressure, we performed a longitudinal study on lambs from single, twin and triplet pregnancies. The lambs were studied from birth until adulthood, including regular blood analyses, measurements of body weight and blood pressure and post-mortem estimation of glomerular numbers. Relative weight differences between multiples and singletons at birth were -28% for twins and -44% for triplets, respectively. Some lambs showed rapid catch-up growth. Total nephron number of twins and triplets was reduced by 21 and 37% with respect to that of singletons (p < 0.01). However, multiples did not show increased blood pressure within the time frame of this study. No gender-specific effect was observed. Plasma concentrations of creatinine, urea, electrolytes or osmolality also did not differ. Our data indicate that the previously reported postnatal blood pressure differences between sheep multiples and singletons are a time-limited phenomenon. During infancy and adolescence, a reduced nephron number in sheep multiples is neither associated with increased blood pressure nor reflected by plasma parameters.
Mühle
Anja
A
Children's Hospital, University of Erlangen-Nuernberg, Loschgestr 15, 91054 Erlangen, Germany.
Mühle
Christiane
C
Amann
Kerstin
K
Dötsch
Jörg
J
Nüsken
Kai-Dietrich
KD
Boltze
Johannes
J
Schneider
Holm
H
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure; D001835:Body Weight; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D004573:Electrolytes / Q000097:blood; D005260:Female; D005317:Fetal Growth Retardation / Q000097:blood / Q000503:physiopathology*; D006973:Hypertension / Q000097:blood / Q000209:etiology* / Q000503:physiopathology; D008297:Male; D009399:Nephrons / Q000254:growth & development*; D009994:Osmolar Concentration; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012307:Risk Factors; D012756:Sheep; D014310:Triplets; D014427:Twins; D014508:Urea / Q000097:blood
D015415:Biomarkers; D004573:Electrolytes; D014508:Urea; D003404:Creatinine
N Engl J Med. 1976 Aug 12;295(7):349-53
934222
Lab Invest. 1991 Jun;64(6):777-84
2046329
Twin Res. 2001 Oct;4(5):385-91
11869493
J Physiol. 2005 May 15;565(Pt 1):137-47
15790663
Arch Pediatr Adolesc Med. 2006 Sep;160(9):977-81
16953023
Res Vet Sci. 1996 Mar;60(2):122-5
8685532
Acta Paediatr. 2006 Oct;95(10):1234-8
16982496
Am J Hypertens. 1988 Oct;1(4 Pt 1):335-47
3063284
J Neuroendocrinol. 2004 Jun;16(6):502-7
15189324
Horm Res. 2007;68(3):113-23
17351325
Nephrol Dial Transplant. 1994;9(7):875
7970135
J Hypertens. 2006 Jul;24(7):1337-43
16794483
Am J Vet Res. 2006 Sep;67(9):1541-5
16948598
Pathol Res Pract. 2000;196(2):111-7
10707368
Am J Physiol Renal Physiol. 2007 Dec;293(6):F1944-9
17898037
Pediatr Nephrol. 1997 Dec;11(6):719-23
9438651
Physiol Rev. 2005 Apr;85(2):571-633
15788706
Kidney Int. 2005 Mar;67(3):822-8
15698422
Kidney Int. 2000 Aug;58(2):770-3
10916101
Nephron. 1998;79(1):28-32
9609458
Am J Obstet Gynecol. 2005 Jan;192(1):272-9
15672036
Acta Physiol Scand. 2002 Oct;176(2):83-90
12354166
Pediatr Res. 2004 May;55(5):769-73
14973179
J Am Soc Nephrol. 2007 Jun;18(6):1688-96
17442788
Pediatr Nephrol. 2001 Apr;16(4):340-5
11354778
Am J Physiol Regul Integr Comp Physiol. 2005 Jan;288(1):R97-103
15374819
J Am Soc Nephrol. 2008 Jan;19(1):151-7
18057216
J Lab Clin Med. 1965 Jan;65:26-39
14260693
BMJ. 1995 Jul 15;311(6998):171-4
7613432
Ethn Dis. 2006 Spring;16(2 Suppl 2):S2-40-5
16774009
Anat Rec. 1992 Feb;232(2):194-201
1546799
BMJ. 1991 Oct 26;303(6809):1019-22
1954451
Am J Physiol Regul Integr Comp Physiol. 2008 Aug;295(2):R583-95
18509101
Pediatr Nephrol. 1994 Apr;8(2):175-80
8018495
Br J Obstet Gynaecol. 1992 Apr;99(4):296-301
1581274
J Am Soc Nephrol. 2003 Sep;14(9):2288-96
12937305
Clin Exp Pharmacol Physiol. 2008 Jul;35(7):730-43
18498533
Kidney Int. 2003 Jun;63(6):2113-22
12753298
J Physiol. 2007 Feb 1;578(Pt 3):871-81
17124269
J Appl Physiol. 1962 Mar;17:343-8
14005589
Pediatr Nephrol. 2000 Dec;15(3-4):192-5
11149109
N Engl J Med. 2003 Jan 9;348(2):101-8
12519920
J Physiol. 2005 May 15;565(Pt 1):19-26
15774527
Reprod Sci. 2008 Jan;15(1):66-74
18212356