Differential stress responses among newly received calves: variations in reductant capacity and Hsp gene expression.
Bovine respiratory disease complex (BRD), a major economic concern to the beef cattle industry all over the world, is triggered by physical, biological and psychological stresses. It is becoming noticeable that the key to reducing BRD appears to be centered at reducing the response to stress. The aims of the present study were to detect individual variations in the stress response of newly received young calves through their leukocyte heat shock protein (Hsp) response, selected neutrophil-related gene expression and oxidative stress, and relate them to pulmonary adhesions at slaughter, an indicative sign of clinical and subclinical episodes of BRD at an early age. Differential expression patterns of Hsp60 and Hsp70A1A were revealed in newly received calves 1 h, 5 h and 1 day after arrival, distinguishing between stress-responsive and non-stress-responsive individuals. Plasma cortisol was also indicative of stress-responsive and non-stress-responsive individuals, 1 h and 5 h after arrival. At the longer term, β-glycan levels were highest 7 days after arrival and significantly correlated with an adhesion-free phenotype at slaughter. Oxidative stress responses, measured through the oxidation products of the exogenous linoleoyl tyrosine (LT) marker, revealed that hydroperoxidation and epoxidation of membranes may readily occur. Based on the LT oxidation products and levels of β-glycan, we present a discriminant analysis model, according to which vulnerable individuals may be predicted at near 100% probability 7 days after arrival. Since clinical signs of BRD may often go undetected in feedlot calves, such a model, after its examination in large-scale experiments, may be a reliable tool for an early prediction of subclinical signs of BRD.
Eitam
Harel
H
Department of Ruminant Science & Genetics, Institute of Animal Science, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, 30095, Israel.
Vaya
Jacob
J
Brosh
Arieh
A
Orlov
Ala
A
Khatib
Soliman
S
Izhaki
Ido
I
Shabtay
Ariel
A
D000818:Animals; D000831:Animals, Newborn; D048090:Bovine Respiratory Disease Complex / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D018834:Chaperonin 60 / Q000378:metabolism*; D016002:Discriminant Analysis; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006854:Hydrocortisone / Q000097:blood; D019041:L-Selectin / Q000235:genetics / Q000378:metabolism; D008041:Linoleic Acids / Q000737:chemistry / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism
D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D008041:Linoleic Acids; C529367:N-linoleoyltyrosine; D011509:Proteoglycans; D018125:Receptors, Transforming Growth Factor beta; D019041:L-Selectin; C071736:betaglycan; D014443:Tyrosine; D020780:Matrix Metalloproteinase 9; D006854:Hydrocortisone
Annu Rev Genet. 1988;22:631-77
2853609
Psychosom Med. 1996 Sep-Oct;58(5):404-12
8902892
Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):770-4
8421714
J Anim Sci. 2006 Feb;84(2):488-98
16424278
J Vet Med Sci. 2004 Apr;66(4):381-7
15133267
Vet J. 2007 May;173(3):605-16
16632387
J Biol Chem. 2002 Apr 26;277(17):15028-34
11836257
J Leukoc Biol. 2004 May;75(5):815-27
14761937
Cell Stress Chaperones. 2009 Jan;14(1):61-9
18548335
Am J Vet Res. 2004 Jun;65(6):860-4
15198229
Am J Vet Res. 1999 Apr;60(4):452-7
10211688
Am J Vet Res. 1984 Oct;45(10):2003-7
6437299
J Anim Sci. 2007 Mar;85(13 Suppl):E81-8
17085721
Comp Funct Genomics. 2005;6(4):244-50
18629190
OMICS. 2007 Winter;11(4):413-34
18092912
Trends Microbiol. 2008 Feb;16(2):55-64
18191570
Cell Stress Chaperones. 2008 Dec;13(4):459-66
18465207
J Anim Sci. 1984 Sep;59(3):576-83
6490547
J Anim Sci. 2007 Aug;85(8):1885-92
17504959
Int Immunol. 2000 Nov;12(11):1539-46
11058573
Lancet. 2003 Aug 9;362(9382):469-76
12927437
Nature. 1992 Oct 22;359(6397):693-9
1436033
Annu Rev Immunol. 1998;16:137-61
9597127
J Leukoc Biol. 2008 Mar;83(3):489-92
18156188
J Anim Sci. 1999 Dec;77(12):3168-75
10641860
Nat Biotechnol. 1998 Sep;16(9):833-8
9743115
J Neuroimmunol. 2002 Dec;133(1-2):1-19
12446003
Vet Immunol Immunopathol. 2001 Dec;83(3-4):213-40
11730931
J Endocrinol. 2004 Dec;183(3):569-83
15590983
Free Radic Res. 2006 Jan;40(1):41-52
16298758
Physiol Genomics. 2006 Dec 13;28(1):97-112
16912069
J Immunol. 1999 Mar 15;162(6):3212-9
10092772
J Dairy Sci. 1993 Sep;76(9):2812-23
8227685
Br Vet J. 1987 Mar-Apr;143(2):166-74
3495314
J Leukoc Biol. 2006 Mar;79(3):425-34
16387837
Cell Cycle. 2004 Sep;3(9):1098-100
15326368
Evolution. 1989 Jan;43(1):223-225
28568501
Vet Res. 2007 Nov-Dec;38(6):809-18
17727806
Lancet Oncol. 2004 Oct;5(10):617-25
15465465
Am J Vet Res. 2000 Jan;61(1):14-9
10630771
Hum Pathol. 2006 Apr;37(4):422-30
16564916
Vet Immunol Immunopathol. 2005 May 15;105(3-4):197-219
15808301
Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):19-29
17512060
J Anim Sci. 2003 Nov;81(11):2847-55
14601889
Cell Stress Chaperones. 2003 Fall;8(3):272-86
14984061
Biomarkers. 2008 Feb;13(1):119-31
17852078
J Am Vet Med Assoc. 1996 Aug 15;209(4):814-8
8756886
FEBS Lett. 2005 Oct 24;579(25):5487-93
16213498
J Anim Sci. 1994 Jul;72(7):1891-8
7928769
Biochem J. 2004 Jul 15;381(Pt 2):413-22
15086314
Can J Comp Med. 1982 Jul;46(3):225-63
6290011