Controlled solvent-exchange deposition of phospholipid membranes onto solid surfaces.
Controlled deposition of lipid bilayers plays a key role in creating supported membranes for biosensing devices and biophysical cell studies. The authors adopt a solvent-exchange method in order to deposit a phospholipid bilayer on solid substrates. The basic concept of deposition is to dissolve phospholipids in isopropanol-water mixtures and to increase water content gradually. Shortly before the onset of the micelle-to-vesicle transition, a lipid bilayer nucleates at the solid surface. They investigate the bulk phase behavior and surface coverage using small angle x-ray scattering and attenuated total reflection-Fourier transform infrared spectroscopy. They find a sequence of transitions from inverted-monomeric-micellar and vesicle phases correlating with an increasing amount of lipid on the adsorption layer. Supported lipid bilayers, prepared using this approach, are homogeneous and fluid.
Hohner
Andreas O
AO
Centre for Nanoscience and Fakultat fur Physik, Ludwig-Maximilians-Universitat, Geschwister-Scholl-Platz 1, Munchen D-80539, Germany.
David
Maria Pamela C
MP
Rädler
Joachim O
JO
D019840:2-Propanol / Q000737:chemistry; D000327:Adsorption; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism; D053838:Scattering, Small Angle; D012997:Solvents / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
D008051:Lipid Bilayers; D010743:Phospholipids; D012997:Solvents; D014867:Water; D019840:2-Propanol; D004134:Dimyristoylphosphatidylcholine