[Establishment and characterization of a human gallbladder carcinoma cell line EH-GB1 originated from a metastatic tumor].
OBJECTIVE
To establish a human gallbladder carcinoma cell line derived from a metastatic gallbladder carcinoma and identify its biological characteristics.


METHODS
Tissue samples were separated from the surgical specimen obtained from a patient with metastatic carcinoma and single-cell suspension was prepared. Then the cells were cultured in DMEM medium supplemented with 15% fetal bovine serum. The morphology of tumor cells was observed under an electron microscope. The cell growth curve was plotted. The tumorigenicity of the cell line was studied by subcutaneous inoculation in SCID mice. The cells were infected by lentiviral vector carrying fluorescent report genes (lenti-GFP and lenti-Red2) separately for expressions of GFP and Red2, respectively.


RESULTS
A novel metastatic gallbladder carcinoma cell line was successfully established and named "EH-GB1". It could be passaged for over 20 generations with typical malignant epithelial morphology and a stable growth cycle of 24 h. Tumors were formed in all of the 10 SCID mice inoculated with EH-GB1 cells subcutaneously, and the tumor cells were tumor marker CA19-9-positive. Continuous expressions of fluorescent report genes were observed in EH-GB1 cells infected by lenti-GFP and lenti-Red2.


CONCLUSIONS
EH-GB1 cells might be the first stable cell line of human gallbladder carcinoma established from a metastatic focus of gallbladder carcinoma. This cell line with continuous expressions of GFP and Red2 might be a novel and perfect experimental model for clinical and basic research on gallbladder carcinoma.
Li
Lin-fang
LF
Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China.
Hu
Huan-zhang
HZ
Liu
Chen
C
Wang
Jing-han
JH
Wu
Hong-ping
HP
Jin
Hua-jun
HJ
Su
Chang-qing
CQ
Jiang
Xiao-qing
XQ
Liu
Jia
J
Gu
Jian-zhong
JZ
Wu
Meng-chao
MC
Qian
Qi-jun
QJ
D000008:Abdominal Neoplasms / Q000378:metabolism / Q000473:pathology / Q000556:secondary*; D034861:Abdominal Wall; D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology* / Q000556:secondary; D000818:Animals; D018395:CA-19-9 Antigen / Q000378:metabolism; D045744:Cell Line, Tumor / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005706:Gallbladder Neoplasms / Q000378:metabolism / Q000473:pathology*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D008875:Middle Aged; D009368:Neoplasm Transplantation
D018395:CA-19-9 Antigen; D049452:Green Fluorescent Proteins