Allergens are transported through the respiratory epithelium.
We used a top-down approach with a wide repertoire of wet laboratory and in silico techniques for analyzing the pathogenesis of early events within the type I allergic reactions. We could show a caveolar-dependent transport of the birch pollen allergen through the respiratory epithelium of allergic patients but not of their healthy controls. The application of discovery-driven methodologies can provide new hypotheses worth further analyses of complex multifactorial diseases such as type I allergy.
Renkonen
Risto
R
Transplantation Laboratory & Infection Biology Research Program, Haartman Institute, University of Helsinki & HUSLAB, Helsinki University Central Hospital, Helsinki, Finland. risto.renkonen@helsinki.fi
Renkonen
Jutta
J
Joenväärä
Sakari
S
Mattila
Pirkko
P
Parviainen
Ville
V
Toppila-Salmi
Sanna
S
D000485:Allergens / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D029662:Betula / Q000276:immunology*; D001692:Biological Transport; D016022:Case-Control Studies; D021941:Caveolae / Q000378:metabolism; D002463:Cell Membrane Permeability; D003228:Conjunctiva / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007428:Intradermal Tests; D007700:Kinetics; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D009297:Nasal Mucosa / Q000276:immunology / Q000378:metabolism*; D011058:Pollen / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding; D040901:Proteomics / Q000379:methods; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism*
D000485:Allergens; D008024:Ligands