Down-regulation of NF-kappaB led to up-regulation of NGF production in mouse osteoblasts.
For investigation of the molecular cascade leading to nerve growth factor (NGF) in mouse osteoblastic MC3T3-E1 cells, pyrrolidine dithiocarbamate (PDTC), an NF-kappaB inhibitor, was utilized. A dose-dependent PDTC-elicited stimulation of NGF production occurred in the cells. PDTC attenuated the NF-kappaB nuclear translocation, whereas PDTC had no effects on the cell proliferation, apoptosis, differentiation, or cell cycle. The cell membrane-permeable SN50 peptide (a specific NF-kappaB inhibitor peptide) also blocked the NF-kappaB translocation to the nucleus, and induced NGF protein in the cells. These findings demonstrate that the suitable suppression of NF-kappaB nuclear translocation induced NGF production in non-neuronal osteoblastic cells.
Mogi
Makio
M
Department of Medicinal Biochemistry, School of Pharmacy, Aichi-Gakuin University, Nagoya, Japan. makio@dpc.aichi-gakuin.ac.jp
Kondo
Ayami
A
D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D049109:Cell Proliferation; D015536:Down-Regulation; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000096:biosynthesis*; D010006:Osteoblasts / Q000378:metabolism*
D016328:NF-kappa B; D020932:Nerve Growth Factor