title: [(Methyl)1-(11)c]-acetate metabolism in hepatocellular carcinoma.
abstract: OBJECTIVE
  Studies have established the value of [(methyl)1-(11)C]-acetate ([(11)C]Act) combined with 2-deoxy-2[(18)F]fluoro-D-glucose (FDG) for detecting hepatocellular carcinoma (HCC) using positron emission tomography (PET). In this study, the metabolic fate of [(11)C]Act in HCC was characterized.
  
  
  METHODS
  Experiments with acetic acid [1-(14)C] sodium salt ([(14)C]Act) were carried out on WCH-17 cells and freshly derived rat hepatocytes. PET scans with [(11)C]Act were also carried out on woodchucks with HCC before injection of [(14)C]Act. The radioactivity levels in different metabolites were quantified with thin-layer chromatography.
  
  
  RESULTS
  In WCH-17 cells, the predominant metabolite was phosphatidylcholine (PC). Regions of HCCs with the highest [(11)C]Act uptake had higher radioactivity accumulation in lipid-soluble compounds than surrounding hepatic tissues. In those regions, PC and triacylglycerol (TG) accumulated more radioactivity than in surrounding hepatic tissues.
  
  
  CONCLUSIONS
  High [(11)C]Act uptake in HCC is associated with increased de novo lipogenesis. PC and TG are the main metabolites into which the radioactive label from [(11)C]Act is incorporated in HCC.
authors.affiliation: Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Salem
authors.forename: Yu
authors.initials: Y
authors.lastname: Kuang
authors.forename: David
authors.initials: D
authors.lastname: Corn
authors.forename: Bernadette
authors.initials: B
authors.lastname: Erokwu
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Kolthammer
authors.forename: Haibin
authors.initials: H
authors.lastname: Tian
authors.forename: Chunying
authors.initials: C
authors.lastname: Wu
authors.forename: Fangjing
authors.initials: F
authors.lastname: Wang
authors.forename: Yanming
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Lee
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes / Q000378:metabolism*; D045744:Cell Line, Tumor; D022781:Hepatocytes / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D049268:Positron-Emission Tomography; D051381:Rats
chemical_list: D000085:Acetates; D002250:Carbon Radioisotopes; C046923:methyl acetate
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