Determination of 3-O- and 4-O-methylated monosaccharide constituents in snail glycans.
The N- and O-glycans of Arianta arbustorum, Achatina fulica, Arion lusitanicus and Planorbarius corneus were analysed for their monosaccharide pattern by reversed-phase HPLC after labelling with 2-aminobenzoic acid or 3-methyl-1-phenyl-2-pyrazolin-5-one and by gas chromatography-mass spectrometry. Glucosamine, galactosamine, mannose, galactose, glucose, fucose and xylose were identified. Furthermore, three different methylated sugars were detected: 3-O-methyl-mannose and 3-O-methyl-galactose were confirmed to be a common snail feature; 4-O-methyl-galactose was detected for the first time in snails.
Stepan
Herwig
H
Department of Chemistry, University of Natural Resources and Applied Life Sciences, Vienna, Austria.
Bleckmann
Christina
C
Geyer
Hildegard
H
Geyer
Rudolf
R
Staudacher
Erika
E
D000818:Animals; D005690:Galactose / Q000737:chemistry; D013058:Mass Spectrometry; D008745:Methylation; D009005:Monosaccharides / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012908:Snails / Q000737:chemistry*
D009005:Monosaccharides; D011134:Polysaccharides; D009584:Nitrogen; D010100:Oxygen; D005690:Galactose