title: Schistosoma japonicum: screening of cercariae cDNA library by specific single-chain antibody against SIEA26-28 ku and immunization experiment of the recombinant plasmids containing the selected genes.
abstract: To obtain the gene encoding SIEA26-28 ku, which has been proven to be a potential anti-schistosomiasis vaccine candidate, screening Schistosoma japonicum (Sj) cercariae cDNA library with soluble specific single-chain antibody (SIEA26-28 ku-scFv) was performed. A large amount of specific single-chain antibody was harvested through construction of recombinant expression vector pET32a/scFv. The protein was purified and characterized. By using this protein (PET32a-scFv) as a probe, S. japonicum cercariae cDNA library was screened. Two strong positive clones were selected, and their eukaryotic recombinant plasmids were constructed. These genes were named as S. japonicum ribosomal protein S4 (SjRPS4) and S. japonicum ribosomal protein L7 (SjRPL7), respectively. Experiments of mice showed that both SjRPS4 and SjRPL7 DNA vaccines could induce significant immunoprotection. Result of these experiments further proved that the specific single-chain antibody is a very valuable tool in screening of cDNA library to get the corresponding molecules.
authors.affiliation: Department of Parasitology, Xiangya Medical School, Central South University, 110 Xiangya Road, Changsha, 410078, People's Republic of China.
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Gao
authors.forename: Shi-ping
authors.initials: SP
authors.lastname: Wang
authors.forename: Zhuo
authors.initials: Z
authors.lastname: He
authors.forename: Ming-chiu
authors.initials: MC
authors.lastname: Fung
authors.forename: Ming-she
authors.initials: MS
authors.lastname: Liu
authors.forename: Lu-xin
authors.initials: LX
authors.lastname: Yu
authors.forename: Xiu-chun
authors.initials: XC
authors.lastname: Chen
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D005260:Female; D015723:Gene Library; D007114:Immunization / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood; D007422:Intestines / Q000469:parasitology; D008099:Liver / Q000469:parasitology; D051379:Mice; D010270:Parasite Egg Count; D010957:Plasmids; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D012269:Ribosomal Proteins / Q000235:genetics / Q000276:immunology; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D057127:Single-Chain Antibodies; D014599:Uterus / Q000469:parasitology; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D015800:Protozoan Proteins; D012269:Ribosomal Proteins; D057127:Single-Chain Antibodies; D019444:Vaccines, DNA; C031798:ribosomal protein S4; C016700:ribosomal protein L7-L12
references.citation: J Histochem Cytochem. 2003 May;51(5):567-74
references.pmid: 12704204
references.citation: Exp Cell Res. 1997 Feb 1;230(2):252-61
references.pmid: 9024784
references.citation: Breast Cancer Res Treat. 2001 Sep;69(1):29-38
references.pmid: 11759826
references.citation: J Immunol. 2001 Jan 1;166(1):498-505
references.pmid: 11123329
references.citation: Zhonghua Fu Chan Ke Za Zhi. 2007 Mar;42(3):187-91
references.pmid: 17537306
references.citation: Int Rev Immunol. 1999;18(5-6):429-48
references.pmid: 10672495
references.citation: Science. 1993 Mar 19;259(5102):1745-9
references.pmid: 8456302
references.citation: Vaccine. 2004 Nov 15;23(1):78-83
references.pmid: 15519710
references.citation: Immunol Cell Biol. 1999 Jun;77(3):197-205
references.pmid: 10361251
references.citation: Zhonghua Xue Ye Xue Za Zhi. 2003 Apr;24(4):185-9
references.pmid: 12864948
references.citation: Sheng Wu Gong Cheng Xue Bao. 2004 May;20(3):342-7
references.pmid: 15971602
references.citation: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Mar;20(2):159-62
references.pmid: 15191715
references.citation: Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1998;16(3):176-80
references.pmid: 12078236
references.citation: Trends Biochem Sci. 1996 May;21(5):164-5
references.pmid: 8871397