title: p38 MAPK regulates Th2 cytokines release in PBMCs in allergic rhinitis rats.
abstract: Th2 cytokines play a pivotal role in the pathogenesis of allergic rhinitis. To investigate the effect of p38 mitogen-activated protein kinase (MAPK) on the production of Th2 cytokines such as IL-4 and IL-5 in allergic rhinitis, a model of allergic rhinitis was established in SD rats. The expression level of p38 MAPK mRNA in PBMCs was detected by means of real time quantitative RT-PCR. The p38 MAPK activity in PBMCs was detected by Western blotting. PBMCs were cultured with various concentrations of p38 MAPK inhibitor SB 239063 or without the treatment, and then IL-4, IL-5 levels of the supernatant were determined by using sandwich ELISA. The results showed that mRNA expression and activity of p38 MAPK in PBMCs were significantly higher in allergic rhinitis rats than in control rats (P<0.05). The p38 MAPK inhibitor SB 239063 decreased the production of IL-4 and IL-5 in a dose-dependent manner. It is concluded that p38 MAPK plays an important role in the pathogenesis of allergic rhinitis which is associated with Th2 cytokines release.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. LJ0901@163.com
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Lisi
authors.initials: L
authors.lastname: Liu
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Cui
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Hongqun
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D007093:Imidazoles / Q000494:pharmacology; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D010047:Ovalbumin; D011743:Pyrimidines / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D006255:Rhinitis, Allergic, Seasonal / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D016207:Cytokines; D007093:Imidazoles; D015848:Interleukin-5; D011743:Pyrimidines; D012333:RNA, Messenger; D015847:Interleukin-4; D010047:Ovalbumin; D048051:p38 Mitogen-Activated Protein Kinases; C406525:SB 239063
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