Heterogeneity in the sensitivity of microtubules of Giardia lamblia to the herbicide oryzalin.
Giardia lamblia is a protozoan that inhabits the small intestine of vertebrates, attaching to the epithelial cells by means of cytoskeletal elements. G. lamblia trophozoites possess several microtubular structures, namely the adhesive disc, the median body, the funis and the four pairs of flagella. Several drugs that target cytoskeletal proteins have been used in the study of cytoskeletal function and dynamics. In this work, we used oryzalin, which binds to alpha-tubulin, as a tool to study the Giardia cytoskeleton. The trophozoites were treated with oryzalin, and its effects were analysed by immunofluorescency, transmission and scanning electron microscopies. Oryzalin inhibited Giardia proliferation. Treated cells were not able to complete cell division and had flagella showing extensive shortening. Strikingly, the drug did not interfere with the adhesive disc, in contrast to what happens when other drugs are used.
Terra
Letícia L
LL
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS, bloco G, Ilha do Fundão, Cidade Universitária, Rio de Janeiro 21949-900, Brazil.
Campanati
Loraine
L
De Souza
Wanderley
W
D000981:Antiprotozoal Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D004136:Dinitrobenzenes / Q000494:pharmacology*; D005407:Flagella / Q000187:drug effects; D016829:Giardia lamblia / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D006540:Herbicides / Q000494:pharmacology*; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D013424:Sulfanilamides / Q000494:pharmacology*
D000981:Antiprotozoal Agents; D004136:Dinitrobenzenes; D006540:Herbicides; D013424:Sulfanilamides; C012465:oryzalin
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