[Hydrogen peroxide inhibits acetylcholinesterase of myometrium sarcolemma].
The action of hydrogen peroxide on acetylcholinesterase enzymatic activity in myometrium sarcolemma fraction is investigated. Hydrogen peroxide (0.1-26 microM), depending on the concentration, suppressed the activity. Acetylcholinesterase proved to be highly sensitive to the action of H2O2, making Ki = 2.4 +/- 0.4 microM, nH = 0.65 +/- 0.08 (n = 4-5). It is established, that hydrogen peroxide in the range of 1.6 - 6.4 microM essentially reduce V(0,max) and K(M). In the presence of dithiothreitole (a reducer of SH-groups of the membrane surface) the investigated substance effect considerably decreased.
Danylovych
Iu V
IuV
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D009215:Myometrium / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D012508:Sarcolemma / Q000187:drug effects* / Q000201:enzymology; D013379:Substrate Specificity; D013552:Swine
D002800:Cholinesterase Inhibitors; D006861:Hydrogen Peroxide; D000110:Acetylcholinesterase; D004229:Dithiothreitol