title: Characteristics of a nickel-albumin binding assay for assessment of myocardial ischaemia.
abstract: BACKGROUND
  The aim of this study was to describe a method to measure ischaemia-induced alterations of the binding capacity of serum albumin to exogenous nickel.
  
  
  METHODS
  We measured the levels of cardiac troponin I (cTnI), serum albumin, ischaemia-modified albumin (IMA) measured by a cobalt-albumin binding assay (CABA), and a nickel-albumin binding assay (NABA) in the following groups: myocardial infarction (n = 32) and non-ischaemic chest pain (n = 64).
  
  
  RESULTS
  IMA, cTnI and NABA levels were higher in the myocardial infarction group. NABA presented a higher ability to discriminate myocardial ischaemia than CABA.
  
  
  CONCLUSIONS
  Patients with myocardial infarction have reduced nickel binding to human serum albumin, and NABA may have an important role as an early marker of myocardial ischaemia.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Sandra Huber
authors.initials: SH
authors.lastname: da Silva
authors.forename: Bruna dos Santos
authors.initials: Bdos S
authors.lastname: Hausen
authors.forename: Dievan Bisognin
authors.initials: DB
authors.lastname: da Silva
authors.forename: Aline Minuzzi
authors.initials: AM
authors.lastname: Becker
authors.forename: Marli Matiko Anraku
authors.initials: MM
authors.lastname: de Campos
authors.forename: Marta Maria Medeiros Frescura
authors.initials: MM
authors.lastname: Duarte
authors.forename: Rafael Noal
authors.initials: RN
authors.lastname: Moresco
mesh_terms: D000368:Aged; D003035:Cobalt / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000175:diagnosis* / Q000378:metabolism; D009532:Nickel / Q000378:metabolism*; D012372:ROC Curve; D012709:Serum Albumin / Q000378:metabolism*; D019210:Troponin I / Q000097:blood; D020107:Troponin T / Q000097:blood
chemical_list: D012709:Serum Albumin; D019210:Troponin I; D020107:Troponin T; D003035:Cobalt; D009532:Nickel