The RING finger domain of Varicella-Zoster virus ORF61p has E3 ubiquitin ligase activity that is essential for efficient autoubiquitination and dispersion of Sp100-containing nuclear bodies.
Varicella zoster virus encodes an immediate-early (IE) protein termed ORF61p that is orthologous to the herpes simplex virus IE protein ICP0. Although these proteins share several functional properties, ORF61p does not fully substitute for ICP0. The greatest region of similarity between these proteins is a RING finger domain. We demonstrate that disruption of the ORF61p RING finger domain by amino acid substitution (Cys19Gly) alters ORF61p intranuclear distribution and abolishes ORF61p-mediated dispersion of Sp100-containing nuclear bodies. In addition, we demonstrate that an intact ORF61p RING finger domain is necessary for E3 ubiquitin ligase activity and is required for autoubiquitination and regulation of protein stability.
Walters
Matthew S
MS
Department of Microbiology and Immunology, College of Physicians and Surgeons, Columbia University, 701 W. 168th St., New York, NY 10032, USA.
Kyratsous
Christos A
CA
Silverstein
Saul J
SJ
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D034961:Antigens, Nuclear / Q000378:metabolism*; D001324:Autoantigens / Q000378:metabolism*; D002467:Cell Nucleus / Q000737:chemistry; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000502:physiology*; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D055550:Protein Stability; D054829:RING Finger Domains; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D034961:Antigens, Nuclear; D001324:Autoantigens; D017874:Immediate-Early Proteins; D014764:Viral Proteins; C073411:protein 61, Varicella-zoster virus; C066642:SP100 protein, human; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1
J Virol. 2008 Sep;82(17):8673-86
18562514
J Virol. 2006 Aug;80(16):8006-18
16873257
Nature. 1970 Aug 15;227(5259):680-5
5432063
Virology. 1977 Apr;77(2):733-49
193259
J Virol. 1992 Dec;66(12):7303-8
1366099
J Virol. 2009 May;83(9):4262-74
19211749
J Virol. 1993 Jul;67(7):4290-5
8389928
J Virol. 2009 Nov;83(22):11502-13
19740981
J Virol. 2009 Oct;83(20):10637-43
19656893
J Virol. 2010 Apr;84(7):3476-87
20106921
J Virol. 2006 Aug;80(16):7995-8005
16873256
Bioessays. 1998 Aug;20(8):660-7
9780840
J Gen Virol. 1986 Nov;67 ( Pt 11):2365-80
3023529
EMBO J. 1985 Aug;4(8):1973-80
2998778
Proc Natl Acad Sci U S A. 1985 Aug;82(16):5265-9
2991915
J Virol. 2000 Feb;74(4):2052-6
10644380
J Virol. 2006 Nov;80(21):10836-46
16956951
Mol Cell Biol. 1985 May;5(5):957-63
2987684
J Virol. 1992 May;66(5):2916-27
1313910
Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):631-6
11805320
Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20912-7
19088197
J Virol. 2002 Jan;76(2):841-50
11752173
J Virol. 2009 Aug;83(15):7560-72
19457996
Virology. 1994 Nov 15;205(1):238-46
7975220