Mug28, a meiosis-specific protein of Schizosaccharomyces pombe, regulates spore wall formation.
The meiosis-specific mug28(+) gene of Schizosaccharomyces pombe encodes a putative RNA-binding protein with three RNA recognition motifs (RRMs). Live observations of meiotic cells that express Mug28 tagged with green fluorescent protein (GFP) revealed that Mug28 is localized in the cytoplasm, and accumulates around the nucleus from metaphase I to anaphase II. Disruption of mug28(+) generated spores with low viability, due to the aberrant formation of the forespore membrane (FSM). Visualization of the FSM in living cells expressing GFP-tagged Psy1, an FSM protein, indicated that mug28Delta cells harbored abnormal FSMs that contained buds, and had a delayed disappearance of Meu14, a leading edge protein. Electron microscopic observation revealed that FSM formation was abnormal in mug28Delta cells, showing bifurcated spore walls that were thicker than the nonbifurcated spore walls of the wild type. Analysis of Mug28 mutants revealed that RRM3, in particular phenylalanin-466, is of primary importance for the proper localization of Mug28, spore viability, and FSM formation. Together, we conclude that Mug28 is essential for the proper maturation of the FSM and the spore wall.
Shigehisa
Akira
A
Department of Molecular Genetics, Osaka University, Suita, Osaka 565-0871, Japan.
Okuzaki
Daisuke
D
Kasama
Takashi
T
Tohda
Hideki
H
Hirata
Aiko
A
Nojima
Hiroshi
H
D020816:Amino Acid Motifs; D019943:Amino Acid Substitution / Q000235:genetics; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002473:Cell Wall / Q000378:metabolism* / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008540:Meiosis; D050505:Mutant Proteins / Q000378:metabolism; D010641:Phenotype; D021381:Protein Transport; D012331:RNA, Fungal / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D012568:Schizosaccharomyces / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism*; D017384:Sequence Deletion / Q000235:genetics; D013172:Spores, Fungal / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors
D018797:Cell Cycle Proteins; C552176:Mug28 protein, S pombe; D050505:Mutant Proteins; D012331:RNA, Fungal; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins
Cell. 1994 Aug 12;78(3):487-98
7520368
Nature. 2002 Dec 5;420(6915):563-73
12466851
Cell Struct Funct. 2003 Oct;28(5):399-417
14745133
Biochim Biophys Acta. 2005 Jul 10;1744(3):438-54
15913810
Mol Biol Cell. 2002 Aug;13(8):2571-84
12181330
DNA Res. 2002 Dec 31;9(6):209-15
12597277
Mol Biol Cell. 2009 Jan;20(1):134-45
18946082
Cell Div. 2007 May 18;2:14
17509158
Mol Biol Cell. 2001 Dec;12(12):3955-72
11739793
Nat Rev Genet. 2004 Apr;5(4):316-23
15131654
Genes Cells. 2002 Feb;7(2):217-31
11895484
Genes Cells. 2003 Jun;8(6):547-58
12786945
Cell Div. 2007 Jun 06;2:17
17550626
Bioessays. 2008 Apr;30(4):338-48
18348251
J Cell Sci. 2004 Jan 26;117(Pt 3):389-96
14702385
J Cell Sci. 2003 Jul 1;116(Pt 13):2721-35
12759375
Eukaryot Cell. 2007 Dec;6(12):2343-53
17951524
Cell Struct Funct. 2006;31(2):181-95
17202724
EMBO J. 2002 Jun 3;21(11):2807-18
12032093
Nucleic Acids Res. 2004 Jun 21;32(11):3325-39
15210864
Science. 2005 Sep 2;309(5740):1559-63
16141072
Eukaryot Cell. 2006 Aug;5(8):1301-13
16896214
Trends Biochem Sci. 1996 Jan;21(1):18-22
8848833
Mol Microbiol. 2000 Oct;38(2):308-21
11069657
Mol Biol Cell. 2001 Apr;12(4):901-17
11294895
Microbiol Mol Biol Rev. 2005 Dec;69(4):565-84
16339736
BMC Genomics. 2006 Nov 30;7:303
17137508
Mol Biol Cell. 2008 Aug;19(8):3544-53
18550796
Nat Genet. 2002 Sep;32(1):143-7
12161753
Mol Gen Genet. 1988 Dec;215(1):81-6
3241624
Cell. 1998 Oct 2;95(1):115-23
9778252
FEBS Lett. 2000 Jul 28;478(1-2):105-8
10922478
EMBO J. 2001 Dec 17;20(24):6946-57
11742972
Nucleic Acids Res. 2001 Jun 1;29(11):2327-37
11376151
Curr Biol. 2005 Nov 22;15(22):2056-62
16303567
Genetics. 2002 Apr;160(4):1439-50
11973299
Chromosome Res. 2007;15(5):523-37
17674143
PLoS One. 2008 Aug 06;3(8):e2887
18682829
Mol Microbiol. 2000 Jan;35(1):79-89
10632879
J Cell Sci. 2005 Jan 15;118(Pt 2):447-59
15654021