Co-culture of primary rat hepatocytes with rat liver epithelial cells enhances interleukin-6-induced acute-phase protein response.
Three different primary rat hepatocyte culture methods were compared for their ability to allow the secretion of fibrinogen and albumin under basal and IL-6-stimulated conditions. These culture methods comprised the co-culture of hepatocytes with rat liver epithelial cells (CC-RLEC), a collagen type I sandwich culture (SW) and a conventional primary hepatocyte monolayer culture (ML). Basal albumin secretion was most stable over time in SW. Fibrinogen secretion was induced by IL-6 in all cell culture models. Compared with ML, CC-RLEC showed an almost three-fold higher fibrinogen secretion under both control and IL-6-stimulated conditions. Induction of fibrinogen release by IL-6 was lowest in SW. Albumin secretion was decreased after IL-6 stimulation in both ML and CC-RLEC. Thus, cells growing under the various primary hepatocyte cell culture techniques react differently to IL-6 stimulation with regard to acute-phase protein secretion. CC-RLEC is the preferred method for studying cytokine-mediated induction of acute-phase proteins, because of the pronounced stimulation of fibrinogen secretion upon IL-6 exposure under these conditions.
Peters
Stephan J A C
SJ
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Vanhaecke
Tamara
T
Papeleu
Peggy
P
Rogiers
Vera
V
Haagsman
Henk P
HP
van Norren
Klaske
K
D000209:Acute-Phase Proteins / Q000378:metabolism*; D000210:Acute-Phase Reaction / Q000473:pathology*; D000418:Albumins / Q000378:metabolism; D000818:Animals; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology*; D005340:Fibrinogen / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D015850:Interleukin-6 / Q000494:pharmacology*; D008099:Liver / Q000166:cytology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
D000209:Acute-Phase Proteins; D000418:Albumins; D015850:Interleukin-6; D005340:Fibrinogen
Biochem J. 1990 Feb 1;265(3):621-36
1689567
Hepatology. 1984 May-Jun;4(3):373-80
6373549
Hepatology. 1988 Sep-Oct;8(5):1010-8
2458307
Res Immunol. 1992 Sep;143(7):755-9
1439151
Surgery. 1986 Aug;100(2):416-23
3738762
Thromb Haemost. 2004 Aug;92(2):234-43
15269818
Ann Surg. 2005 Jan;241(1):125-33
15622000
J Immunol. 1990 May 15;144(10):3804-8
2110214
Biochem Biophys Res Commun. 1999 May 27;259(1):178-84
10334936
Proc Nutr Soc. 1989 Sep;48(3):347-54
2482490
Immunol Today. 1994 Feb;15(2):74-80
7512342
Anal Biochem. 1993 Aug 15;213(1):29-33
8238878
J Pathol. 1997 Mar;181(3):257-66
9155709
World J Gastroenterol. 2004 Mar 1;10(5):699-702
14991941
Eur J Cell Biol. 1992 Aug;58(2):411-7
1425776
Biochem Biophys Res Commun. 1984 Apr 30;120(2):311-7
6203519
Methods Mol Biol. 1998;107:279-94
14577237
Am J Pathol. 1993 Aug;143(2):538-44
8342601
Biol Chem Hoppe Seyler. 1994 Nov;375(11):779-83
7695840
Surgery. 1985 Sep;98(3):388-95
3898449
J Immunol. 1991 May 1;146(9):3032-7
1707930
Toxicol In Vitro. 2007 Oct;21(7):1325-31
17507196
Am J Physiol. 1997 Nov;273(5):E898-902
9374674
J Biol Chem. 1987 Jan 5;262(1):438-45
2432067
Cytokine. 1992 Jan;4(1):18-23
1319762
Methods Mol Biol. 2006;320:239-246
27699670
Toxicology. 1993 Oct 5;82(1-3):193-208
8236274
Hepatology. 1990 Nov;12(5):1179-86
1699862
Biotechnol Bioeng. 1997 Dec 20;56(6):706-11
18642343
Miner Electrolyte Metab. 1999 Jul-Dec;25(4-6):228-33
10681645
Methods Mol Biol. 1998;107:295-301
14577238
Mol Biol Med. 1990 Apr;7(2):147-59
1692952
J Clin Lab Anal. 1999;13(6):273-9
10633294
J Immunol Methods. 1990 Nov 6;134(1):101-6
1700017
FEBS Lett. 1989 Jan 2;242(2):237-9
2464504
J Immunol. 1989 Feb 1;142(3):808-12
2464026
Methods Mol Biol. 1998;107:303-9
14577239
Toxicol In Vitro. 1990;4(4-5):415-27
20702206
Ann Surg. 1998 Feb;227(2):249-54
9488524
Methods Mol Biol. 1998;107:311-7
14577240
Am J Physiol. 1998 Jul;275(1):R203-11
9688980
J Clin Lab Anal. 1999;13(6):280-6
10633295
Biochem J. 2000 Dec 15;352 Pt 3:913-9
11104703
Cell Biol Int. 2000;24(2):109-14
10772770
Am J Pathol. 1995 Jun;146(6):1508-20
7778689
Cell Tissue Res. 2004 Jul;317(1):45-56
15185140