A vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor.
Inducible resistance to the glycopeptide antibiotic vancomycin requires expression of vanH, vanA and vanX, controlled by a two-component regulatory system consisting of a receptor histidine kinase, VanS, and a response regulator, VanR. The identity of the VanS receptor ligand has been debated. Using a synthesized vancomycin photoaffinity probe, we show that vancomycin directly binds Streptomyces coelicolor VanS (VanSsc) and this binding is correlated with resistance and required for vanH, vanA and vanX gene expression.
Koteva
Kalinka
K
M.G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
Hong
Hee-Jeon
HJ
Wang
Xiao Dong
XD
Nazi
Ishac
I
Hughes
Donald
D
Naldrett
Mike J
MJ
Buttner
Mark J
MJ
Wright
Gerard D
GD
D000071677:Histidine Kinase; D015335:Molecular Probes; D019920:Photoaffinity Labels; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D011955:Receptors, Drug / Q000378:metabolism*; D014640:Vancomycin / Q000378:metabolism*
D015335:Molecular Probes; D019920:Photoaffinity Labels; D011955:Receptors, Drug; D014640:Vancomycin; D011494:Protein Kinases; D000071677:Histidine Kinase
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