title: Simultaneous determination of hydrolysable tannins in the petals of Rosa rugosa and allied plants.
abstract: The petals of Rosa rugosa and allied plants for medicinal use contain abundant hydrolysable tannins, and they show remarkable biological activities. The activities are dependent on the structures of the hydrolysable tannins, so their contents and compositions are essential for evaluation of medicinal potency. Therefore, we optimized the simultaneous quantitative determination of the hydrolysable tannins using ultra-performance liquid chromatography. A column of ethylene bridged hybrid (BEH) phenyl (C(6) alkyl phenyl group as solid-phase modification) was shown to be most effective for the separation of hydrolysable tannins isolated from R. rugosa and related compounds when the column temperature was kept under 25 degrees C. The efficacy of the BEH phenyl column might be due to the interaction between solid phase and phenolic ester groups of hydrolysable tannins such as galloyl, hexahydroxydiphenoyl and valoneoyl groups. The relation between the retention times on the BEH phenyl column and the column temperature was demonstrated to depend on the structural characteristics of hydrolysable tannins.
authors.affiliation: Kitami Institute of Technology, 165 Koen-cho, Kitami 090-8507, Japan.
authors.forename: Sarangowa
authors.initials: S
authors.lastname: Ochir
authors.forename: ByoungJae
authors.initials: B
authors.lastname: Park
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishizawa
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kanazawa
authors.forename: Minoru
authors.initials: M
authors.lastname: Funaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamagishi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D035264:Flowers / Q000737:chemistry*; D047348:Hydrolyzable Tannins / Q000737:chemistry*; D015394:Molecular Structure; D027826:Rosa / Q000737:chemistry*
chemical_list: D047348:Hydrolyzable Tannins
references.citation: Biosci Biotechnol Biochem. 2001 Mar;65(3):542-7
references.pmid: 11330666
references.citation: Biosci Biotechnol Biochem. 2008 Mar;72(3):773-7
references.pmid: 18323655