The effect of grape seed extracts on serum paraoxonase activities in streptozotocin-induced diabetic rats.
Procyanidins, a group of flavonoids, are oligomeric forms of catechins that are abundant in red wine, grapes, cocoa, and apples. Paraoxonase acts as an antioxidant enzyme and protects low-density lipoprotein-cholesterol against oxidation. In our study we aimed to evaluate the effects of grape seed extract (GSE) on paraoxonase activities in streptozotocin-induced diabetic rats. Our study included four groups of rats: Group I (n = 8), control; Group II (n = 10), GSE-supplemented; Group III (n = 6), streptozotocin-induced diabetic; and Group IV (n = 7), GSE-supplemented diabetic rats. Serum paraoxonase activities were determined with a spectrophotometric method. Paraoxonase activities in Group III were significantly lower than in the other three groups (P < .001, P < .001, and P = .005 for Groups I, II, and IV, respectively), and Group IV showed increased paraoxonase activities compared to Group III (P = .005). This is the first study to show an association between paraoxonase status and GSE supplementation and demonstrated that GSE increased paraoxonase activities. This beneficial effect of GSE was more obvious in the diabetic group, which was more prone to atherosclerotic events compared to the healthy population.
Kiyici
Aysel
A
Department of Biochemistry, Meram Medical Faculty, Selcuk University, Konya, Turkey.
Okudan
Nilsel
N
Gökbel
Hakki
H
Belviranli
Muaz
M
D000818:Animals; D043303:Aryldialkylphosphatase / Q000097:blood*; D003920:Diabetes Mellitus / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology; D004195:Disease Models, Animal; D056604:Grape Seed Extract / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000009:adverse effects
D056604:Grape Seed Extract; D013311:Streptozocin; D043303:Aryldialkylphosphatase
Metabolism. 2006 Sep;55(9):1201-6
16919539
Am J Physiol Regul Integr Comp Physiol. 2005 Sep;289(3):R771-5
16105821
Metabolism. 2003 Oct;52(10):1250-7
14564675
J Med. 2000;31(5-6):227-46
11508317
Clin Biochem. 2008 Jan;41(1-2):1-9
17920578
Croat Med J. 2001 Apr;42(2):146-50
11259735
Int J Clin Pract. 2006 Nov;60(11):1484-92
17073843
J Toxicol Environ Health. 1993 Oct-Nov;40(2-3):337-46
7693961
Am J Hum Genet. 1983 Nov;35(6):1126-38
6316781
Biochem Biophys Res Commun. 2007 Dec 7;364(1):157-62
17936248
Arterioscler Thromb Vasc Biol. 2004 May;24(5):816-23
14976002
Endocrinology. 2004 Nov;145(11):4985-90
15271880
Genomics. 1996 May 1;33(3):498-507
8661009
Atherosclerosis. 1999 Jan;142(1):139-49
9920515
Eur J Clin Invest. 2006 Nov;36(11):779-87
17032345
Am J Clin Nutr. 2005 Jan;81(1 Suppl):243S-255S
15640487
Acta Diabetol. 2009 Sep;46(3):239-42
18830558
Clin Biochem. 2010 Apr;43(6):553-8
20026099
Res Commun Mol Pathol Pharmacol. 1997 Feb;95(2):179-89
9090754
Heart Vessels. 2007 May;22(3):158-64
17533519
Atherosclerosis. 1998 Aug;139(2):341-9
9712341
Curr Opin Lipidol. 1996 Apr;7(2):69-76
8743898
Arterioscler Thromb Vasc Biol. 2001 Apr;21(4):473-80
11304460
Mutat Res. 2003 Feb-Mar;523-524:87-97
12628506
J Med Food. 2001 Spring;4(1):17-26
12639284
Metabolism. 2000 Nov;49(11):1400-5
11092501
Biosci Biotechnol Biochem. 2006 Sep;70(9):2104-11
16960388
J Biomed Biotechnol. 2004;2004(5):272-278
15577189
J Mol Cell Cardiol. 1999 Jun;31(6):1289-97
10371703
Atherosclerosis. 1999 Aug;145(2):421-2
10488973
Atherosclerosis. 1993 Dec;104(1-2):129-35
8141836
J Womens Health (Larchmt). 2009 Sep;18(9):1435-9
19698032
Toxicology. 2000 Aug 7;148(2-3):187-97
10962138
Free Radic Biol Med. 2003 Jun 15;34(12):1563-74
12788476
Atherosclerosis. 2005 Jun;180(2):389-97
15910867
Arterioscler Thromb Vasc Biol. 1999 Feb;19(2):330-5
9974415