New pyrano-pyrone from Goniothalamus tamirensis enhances the proliferation and differentiation of osteoblastic MC3T3-E1 cells.
The new pyrano-pyrone, (+)-8-epi-9-deoxygoniopypyrone (1) and (+)-9-deoxygoniopypyrone (2) were isolated from a chloroform extract of Goniothalamus tamirensis leaves. Their absolute stereostructures were discussed and confirmed by using infrared (IR), Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), one (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectra, Mosher's method, and comparison with the known compounds leiocapin A (3), deoxygoniopypyrone A (4), and (-)-8-epi-9-deoxygoniopypyrone (5). At concentrations of 2.67 microM, compounds 1 and 2 significantly increased the growth of osteoblastic MC3T3-E1 cells and caused a significant elevation of collagen content, alkaline phosphatase activity, and nodule mineralization in the cells (p<0.05). Our data suggest that the enhancement of osteoblast function by 1 and 2 may result in the prevention of osteoporosis.
Tai
Bui Huu
BH
College of Pharmacy, Chungnam National University, Daejeon, Korea.
Huyen
Vu Thi
VT
Huong
Tran Thu
TT
Nhiem
Nguyen Xuan
NX
Choi
Eun-Mi
EM
Kim
Jeong Ah
JA
Long
Pham Quoc
PQ
Cuong
Nguyen Manh
NM
Kim
Young Ho
YH
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism; D054335:Goniothalamus / Q000737:chemistry*; D008968:Molecular Conformation; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011753:Pyrones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
D011753:Pyrones; D003094:Collagen; D000469:Alkaline Phosphatase