title: Asymmetric partitioning of anions in lysozyme dispersions.
abstract: An aqueous dispersion of lysozyme and sodium dodecyl sulfate phase separates below the cloud point. Two liquid phases are formed. At pH < pI electrolytes change the cloud point temperatures (T(c)). These follow an inverse Hofmeister series. Anions partition asymmetrically between the two phases. At fixed cation, the partitioning depends on the specific anion. In a system of finite volume, a concentrated dispersion of a protein is able to act as a chemical "sponge". No active pump is necessary to maintain concentration differences between concentrated and dilute dispersions.
authors.affiliation: Department of Chemistry and CSGI, University of Florence, 50019 Sesto Fiorentino, Florence, Italy. pln@csgi.unifi.it
authors.forename: Pierandrea
authors.initials: P
authors.lastname: Lo Nostro
authors.forename: Niccolò
authors.initials: N
authors.lastname: Peruzzi
authors.forename: Mirko
authors.initials: M
authors.lastname: Severi
authors.forename: Barry W
authors.initials: BW
authors.lastname: Ninham
authors.forename: Piero
authors.initials: P
authors.lastname: Baglioni
mesh_terms: D000327:Adsorption; D004573:Electrolytes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D016623:Ion Pumps / Q000378:metabolism; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D044366:Transition Temperature
chemical_list: D004573:Electrolytes; D016623:Ion Pumps; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D009113:Muramidase