Effect of solvent polarity on the fluorescence quenching of biologically active 5BAMC by aniline in binary solvent mixtures.
The fluorescence quenching of 5, 6-benzo-4-azidomethyl coumarin (5BAMC) by aniline have been carried in different solvent mixtures of benzene (BN) and acetonitrile (AN) at room temperature by steady state fluorescence measurements. The quenching is found to be appreciable and a positive deviation from linearity was observed in the Stern-Volmer plot for the fluorophore in all the solvent mixtures. Various parameters for the quenching process have been determined using the extended S-V equation and have been found to be dependent on the solvent polarity. Further, with the use of finite sink approximation model, it is concluded that the bimolecular reactions quenching reactions are diffusion limited, and the distance parameter R' and mutual diffusion coefficient D are estimated independently.
Melavanki
Raveendra M
RM
Department of Physics, M S Ramaiah Institute of Technology, Bangalore, 560054, Karnataka, India. melavanki74@rediffmail.com
Kusanur
Raviraj A
RA
Kadadevaramath
Jagadish S
JS
Kulkarni
Manohar V
MV
D000097:Acetonitriles / Q000737:chemistry*; D000814:Aniline Compounds / Q000737:chemistry*; D001554:Benzene / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry*; D004058:Diffusion; D005453:Fluorescence; D012997:Solvents / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
C556863:5,6-benzo-4-azidomethyl coumarin; D000097:Acetonitriles; D000814:Aniline Compounds; D003374:Coumarins; D012997:Solvents; D001554:Benzene; C023650:aniline; C032159:acetonitrile
Spectrochim Acta A Mol Biomol Spectrosc. 2001 Jan;57(1):41-7
11209865
J Am Chem Soc. 1948 Feb;70(2):717-21
18907778