title: The SEPALLATA-like gene OsMADS34 is required for rice inflorescence and spikelet development.
abstract: Grass plants develop distinct inflorescences and spikelets that determine grain yields. However, the mechanisms underlying the specification of inflorescences and spikelets in grasses remain largely unknown. Here, we report the biological role of one SEPALLATA (SEP)-like gene, OsMADS34, in controlling the development of inflorescences and spikelets in rice (Oryza sativa). OsMADS34 encodes a MADS box protein containing a short carboxyl terminus without transcriptional activation activity in yeast cells. We demonstrate the ubiquitous expression of OsMADS34 in roots, leaves, and primordia of inflorescence and spikelet organs. Compared with the wild type, osmads34 mutants developed altered inflorescence morphology, with an increased number of primary branches and a decreased number of secondary branches. In addition, osmads34 mutants displayed a decreased spikelet number and altered spikelet morphology, with lemma/leaf-like elongated sterile lemmas. Moreover, analysis of the double mutant osmads34 osmads1 suggests that OsMADS34 specifies the identities of floral organs, including the lemma/palea, lodicules, stamens, and carpel, in combination with another rice SEP-like gene, OsMADS1. Collectively, our study suggests that the origin and diversification of OsMADS34 and OsMADS1 contribute to the origin of distinct grass inflorescences and spikelets.
authors.affiliation: School of Life Science and Biotechnology , Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Xingchun
authors.initials: X
authors.lastname: Gao
authors.forename: Wanqi
authors.initials: W
authors.lastname: Liang
authors.forename: Changsong
authors.initials: C
authors.lastname: Yin
authors.forename: Shenmin
authors.initials: S
authors.lastname: Ji
authors.forename: Hongmei
authors.initials: H
authors.lastname: Wang
authors.forename: Xiao
authors.initials: X
authors.lastname: Su
authors.forename: Chunce
authors.initials: C
authors.lastname: Guo
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Kong
authors.forename: Hongwei
authors.initials: H
authors.lastname: Xue
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D035264:Flowers / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics
chemical_list: D026161:MADS Domain Proteins; D010940:Plant Proteins; D018749:RNA, Plant
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