title: Direct pathogen detection from swab samples using a new high-throughput sequencing technology.
abstract: The detection of emerging infectious diseases has been a continuing concern, especially with the novel influenza A (H1N1) viral pandemic of 2009. In the present study, we validated a 'second-generation' parallel sequencing platform for viral detection in swab samples collected during recent influenza virus infections in Beijing. This operation yielded millions of valid reads per sample and resulted in an almost complete spectrum of nucleotide information. Importantly, novel A (H1N1) and seasonal A (H3N2) influenza virus-derived sequences were detected without prior knowledge or use of genetic information in advance, suggesting that this approach could be a valuable tool for diagnosing emerging infectious diseases.
authors.affiliation: State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: H
authors.initials: H
authors.lastname: Yongfeng
authors.forename: Y
authors.initials: Y
authors.lastname: Fan
authors.forename: D
authors.initials: D
authors.lastname: Jie
authors.forename: Y
authors.initials: Y
authors.lastname: Jian
authors.forename: Z
authors.initials: Z
authors.lastname: Ting
authors.forename: S
authors.initials: S
authors.lastname: Lilian
authors.forename: Q
authors.initials: Q
authors.lastname: Jin
mesh_terms: D021821:Communicable Diseases, Emerging / Q000175:diagnosis / Q000821:virology; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D014773:Virology / Q000379:methods*
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