title: Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans.
abstract: Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous d-lactate dehydrogenase expression.
authors.affiliation: Groningen Biomolecular Sciences and Biotechnology Institute, Department of Genetics, University of Groningen, Kerklaan 30, 9751 NN Haren, Netherlands.
authors.forename: Akos T
authors.initials: AT
authors.lastname: Kovács
authors.forename: Mariska
authors.initials: M
authors.lastname: van Hartskamp
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
authors.forename: Richard
authors.initials: R
authors.lastname: van Kranenburg
mesh_terms: D001407:Bacillus / Q000235:genetics*; D001709:Biotechnology / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods; D055786:Gene Knockout Techniques / Q000379:methods; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D005827:Genetics, Microbial / Q000379:methods*; D045022:Lactate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D045022:Lactate Dehydrogenases; C071195:D-lactate dehydrogenase; D001616:beta-Galactosidase
references.citation: Appl Microbiol Biotechnol. 2008 Mar;78(3):449-54
references.pmid: 18188553
references.citation: J Biosci Bioeng. 2006 Feb;101(2):172-7
references.pmid: 16569615
references.citation: Curr Opin Microbiol. 2004 Dec;7(6):579-86
references.pmid: 15556029
references.citation: Appl Environ Microbiol. 2008 Sep;74(17):5556-62
references.pmid: 18641148
references.citation: J Bacteriol. 1991 Nov;173(21):6678-85
references.pmid: 1938874
references.citation: Appl Environ Microbiol. 1999 Apr;65(4):1384-9
references.pmid: 10103226
references.citation: Appl Environ Microbiol. 2007 Feb;73(4):1126-35
references.pmid: 17142375
references.citation: Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):8063-8
references.pmid: 10869437
references.citation: Appl Environ Microbiol. 2003 Jan;69(1):399-407
references.pmid: 12514021
references.citation: Appl Biochem Biotechnol. 2009 May;155(1-3):379-85
references.pmid: 19156365
references.citation: Appl Environ Microbiol. 2004 Nov;70(11):6809-15
references.pmid: 15528548
references.citation: Plasmid. 2007 Jul;58(1):13-22
references.pmid: 17215040
references.citation: Nat Biotechnol. 2007 Apr;25(4):443-5
references.pmid: 17351616
references.citation: Appl Microbiol Biotechnol. 2008 Apr;78(5):751-8
references.pmid: 18247027
references.citation: Syst Appl Microbiol. 2004 Feb;27(1):50-60
references.pmid: 15053321
references.citation: J Gen Microbiol. 1987 Mar;133(3):475-81
references.pmid: 3116160
references.citation: Plant J. 1995 Apr;7(4):649-59
references.pmid: 7742860
references.citation: Appl Environ Microbiol. 1994 Feb;60(2):587-93
references.pmid: 8135517
references.citation: Appl Environ Microbiol. 1995 Nov;61(11):3775-80
references.pmid: 8526485
references.citation: Appl Environ Microbiol. 1995 Jul;61(7):2540-7
references.pmid: 7618865
references.citation: Infect Immun. 2003 Feb;71(2):801-13
references.pmid: 12540560
references.citation: Appl Environ Microbiol. 2009 Jan;75(2):462-7
references.pmid: 19011066
references.citation: J Appl Microbiol. 2005;98(3):761-7
references.pmid: 15715880