Imaging gold nanoparticles in living cell environments using heterodyne digital holographic microscopy.
This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environments. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample's scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cell environments.
Warnasooriya
N
N
Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris, Institut Langevin, UMR 7587 CNRS, 10 rue Vauquelin, 75231 Paris Cedex 05, France.
Joud
F
F
Bun
P
P
Tessier
G
G
Coppey-Moisan
M
M
Desbiolles
P
P
Atlan
M
M
Abboud
M
M
Gross
M
M
D000818:Animals; D006046:Gold / Q000032:analysis*; D006696:Holography / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000648:ultrastructure*; D012815:Signal Processing, Computer-Assisted
D006046:Gold