Skeletal advance and arrest in giant non-metamorphosing African clawed frog tadpoles (Xenopus laevis: Daudin).
This study examines the skeletons of giant non-metamorphosing (GNM) Xenopus laevis tadpoles, which arrest their development indefinitely before metamorphosis, and grow to excessively large sizes in the absence of detectable thyroid glands. Cartilage growth is isometric; however, chondrocyte size is smaller in GNM tadpoles than in controls. Most cartilages stain weakly with alcian blue, and several cartilages are calcified (unlike controls). However, cartilages subjacent to periosteum-derived bone retain strong affinities for alcian blue, indicating a role for periosteum-derived bone in the retention of glycosaminoglycans during protracted larval growth. Bone formation in the head, limb, and axial skeletons is advanced in comparison with stage-matched controls, but arrests at various mid-metamorphic states. Both dermal and periosteum-derived bones grow to disproportionately large sizes in comparison to controls. Additionally, mature monocuspid teeth form in several GNM tadpoles. Advances in skeletal development are attributable to the old ages and large sizes of these tadpoles, and reveal unexpected developmental potentials of the pre-metamorphic skeleton.
Kerney
Ryan
R
Biology Department, Dalhousie University, Halifax, NS, Canada. ryankerney@gmail.com
Wassersug
Richard
R
Hall
Brian K
BK
D000818:Animals; D001846:Bone Development / Q000502:physiology; D001842:Bone and Bones / Q000196:embryology*; D002113:Calcification, Physiologic; D002356:Cartilage / Q000196:embryology; D048429:Cell Size; D019902:Chondrocytes / Q000166:cytology; D005877:Gigantism / Q000196:embryology / Q000503:physiopathology; D006614:Hindlimb / Q000196:embryology / Q000502:physiology; D007814:Larva / Q000254:growth & development; D012886:Skull / Q000196:embryology; D013963:Thyroid Hormones / Q000502:physiology; D014070:Tooth / Q000196:embryology; D014982:Xenopus laevis / Q000196:embryology* / Q000254:growth & development / Q000502:physiology
D013963:Thyroid Hormones
J Exp Zool. 1959 Feb;140:1-17
14404190
J Morphol. 1995 Feb;223(2):125-148
29865294
J Morphol. 1992 Oct;214(1):1-41
1433306
Anat Embryol (Berl). 1988;178(3):219-27
3414976
J Anat. 2007 Dec;211(6):754-68
18045351
J Exp Biol. 2004 May;207(Pt 12):2133-45
15143146
Proc Natl Acad Sci U S A. 2005 Aug 30;102(35):12455-8
16129821
Experientia. 1989 Sep 15;45(9):872-5
2776858
Dev Growth Differ. 2003 Aug;45(4):321-5
12950273
Acta Anat (Basel). 1980;106(1):129-40
7415784
Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10739-44
11517345
J Morphol. 2007 Mar;268(3):194-223
17278133
Integr Comp Biol. 2008 Nov;48(5):681-96
21669824
Dev Biol. 2001 Dec 15;240(2):433-42
11784074
Dev Biol. 1969 Nov;20(5):387-410
4187624
Endocrinology. 2003 May;144(5):1979-83
12697705
Bioessays. 2009 Mar;31(3):287-99
19260024
Dev Biol. 1993 Aug;158(2):414-29
8344460
Genes Dev. 2002 Apr 1;16(7):870-9
11937494
Genes Dev. 2002 Apr 1;16(7):859-69
11937493
Bioessays. 1996 May;18(5):391-9
8639162
Clin Endocrinol (Oxf). 2004 Sep;61(3):285-98
15355444
J Morphol. 1979 Dec;162(3):453-63
529294
Mol Endocrinol. 2007 Aug;21(8):1893-904
17488972
J Anat. 1979 Sep;129(Pt 2):323-42
500489
S Afr J Med Sci. 1950 Jul;15(1-2):11-14
14776521
Proc Natl Acad Sci U S A. 2000 Jan 4;97(1):190-4
10618393
J Craniofac Genet Dev Biol. 1990;10(3):263-75
2262537
Dev Biol. 1999 Nov 15;215(2):243-52
10545234
J Morphol. 2009 Feb;270(2):205-20
18946869
Anat Rec. 1973 May;176(1):49-64
4703519
J Embryol Exp Morphol. 1986 Apr;93:133-52
3734681
Dev Biol. 2007 Jun 1;306(1):20-33
17449026
J Anat. 2003 Nov;203(5):435-50
14635799
J Exp Zool. 1988 May;246(2):156-70
3392514
Evolution. 2002 Mar;56(3):628-41
11989691
J Anat. 1989 Feb;162:149-55
2808112
Science. 1973 Dec 28;182(4119):1305-14
17733097
Proc Natl Acad Sci U S A. 1990 Sep;87(18):7090-4
2402492
J Morphol. 1989 Jun;200(3):301-319
29865644
Rev Endocr Metab Disord. 2006 Dec;7(4):265-71
17200892
Dev Biol. 2008 Sep 1;321(1):162-74
18602913