[The approaches for making acute-on-chronic liver failure in rat].
OBJECTIVE
To study the approaches for making acute-on-chronic hepatic failure model in rat.


METHODS
SD rats were intraperitoneally injected with 50% CCl4 (Carbon tetrachloride) olive solution every three days for 6 or 10 weeks. Then they were divided into two groups randomly and injected with D-galactosamine (D-gal) at a dose of 2 g/kg, lipopolysaccharide (LPS) at a dose of 100 pg/kg and D-gal at a dose of 0.5 g/kg BW respectively. The levels of serum ALT, AST and TBil were detected,and histopathological changes were observed to evaluate these two models.


RESULTS
After injection of 50% CCl4 olive solution intraperitoneally for 6 weeks, liver fibrosis happened in SD rats, 10 weeks, cirrhosis happened. Pelleta necrosis and massive or submassive necrosis was seen administration of two reagents mentioned above.


CONCLUSIONS
By injecting of 50% CCl4 olive solution intraperitoneally, acute-on-chronic liver failure model could be induced by D-gal, LPS/D-gal in rats.
Hou
Wei
W
Beijing YouAn Hospital Capital Medical University, Beijing 100069, China.
Piao
Zheng-fu
ZF
Zhang
Hai-yan
HY
Liu
Zhao
Z
Meng
Qing-hua
QH
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D002251:Carbon Tetrachloride / Q000009:adverse effects; D004195:Disease Models, Animal; D005688:Galactosamine / Q000009:adverse effects; D006801:Humans; D008070:Lipopolysaccharides / Q000009:adverse effects; D017114:Liver Failure, Acute / Q000139:chemically induced* / Q000201:enzymology / Q000401:mortality / Q000473:pathology; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D008070:Lipopolysaccharides; D005688:Galactosamine; D002251:Carbon Tetrachloride; D000410:Alanine Transaminase