Detection and quantitative analysis of small RNAs by PCR.
Increasing lines of evidence indicate that small non-coding RNAs including miRNAs, piRNAs, rasiRNAs, 21U endo-siRNAs, and snoRNAs are involved in many critical biological processes. Functional studies of these small RNAs require a simple, sensitive, and reliable method for detecting and quantifying levels of small RNAs. Here, we describe such a method that has been widely used for the validation of cloned small RNAs and also for quantitative analyses of small RNAs in both tissues and cells.
Ro
Seungil
S
Department of Physiology and Cell Biology, University of Nevada School of Medicine, Anderson Biomedical Science, Reno, NV, USA.
Yan
Wei
W
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D026723:Polyadenylation / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D034741:RNA, Small Interfering / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D048348:Reverse Transcription / Q000235:genetics
D018076:DNA, Complementary; D034741:RNA, Small Interfering
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