T-cell receptor early signalling complex activation in response to interferon-alpha receptor stimulation.
Signalling through the IFNalphaR (interferon-alpha receptor) and TCR (T-cell receptor) in Jurkat T lymphocytes results in distinct immune responses. Despite this both receptors elicit ERK (extracellular-signal-regulated kinase)/MAPK (mitogen-activated protein kinase) phosphorylation. Vav and Slp76 are shown to be required for IFNalpha (interferon-alpha)-stimulated ERK activity. These form a subset of proteins which behave identically on stimulation of both receptors. TCR deletion abrogates IFNalphaR-stimulated MAPK activity, whereas the canonical JAK/STAT (Janus kinase/signal transducer and activator of transcription) pathway is unaffected. Thus recruitment of the intact TCR ESC (early signalling complex) is necessary for this downstream MAPK response. Despite using a common ESC, stimulation of the IFNalphaR does not produce the transcriptional response associated with TCR. Up-regulation of the MAPK pathway by IFNalphaR might be important to ensure that the cell responds to only one stimulant.
Stevens
Claire N
CN
Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E6BT, UK.
Simeone
Ann-Marie
AM
John
Susan
S
Ahmed
Zamal
Z
Lucherini
Orso M
OM
Baldari
C Tatiana
CT
Ladbury
John E
JE
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D053633:Receptor, Interferon alpha-beta / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D014162:Transfection
D048868:Adaptor Proteins, Signal Transducing; D010750:Phosphoproteins; D011948:Receptors, Antigen, T-Cell; C092947:SLP-76 signal Transducing adaptor proteins; D053633:Receptor, Interferon alpha-beta; D020928:Mitogen-Activated Protein Kinases
Eur J Immunol. 2000 Sep;30(9):2620-31
11009096
Mol Cell Biol. 2006 Oct;26(19):7155-66
16980618
Cell Immunol. 1999 Mar 15;192(2):133-9
10087181
Nature. 1997 Dec 11;390(6660):629-32
9403695
Oncogene. 1996 Aug 15;13(4):767-75
8761298
Nature. 1997 Jan 9;385(6612):169-72
8990121
Nature. 1985 Aug 15-21;316(6029):606-9
4033759
Immunity. 2006 Aug;25(2):213-24
16919488
Science. 1984 Jun 22;224(4655):1312-6
6427923
Acc Chem Res. 2003 Jun;36(6):410-6
12809527
Cell. 1995 Jul 28;82(2):241-50
7543024
Science. 1995 Sep 22;269(5231):1721-3
7569900
Oncogene. 2000 May 15;19(21):2628-37
10851062
Mol Cell Proteomics. 2005 Jun;4(6):721-30
15659558
Nat Immunol. 2005 Dec;6(12):1253-62
16273097
Biologicals. 1998 Mar;26(1):1-5
9637743
Eur J Biochem. 1997 Apr 1;245(1):84-90
9128727
Curr Opin Immunol. 2005 Jun;17(3):267-74
15886116
J Biol Chem. 2001 Feb 23;276(8):5916-23
11113155
J Exp Med. 1996 Sep 1;184(3):1161-6
9064333
Science. 1998 Jul 17;281(5375):413-6
9665884
Cell. 2000 Sep 1;102(5):625-33
11007481
J Biol Chem. 1999 Oct 15;274(42):30127-31
10514501
Science. 1988 May 27;240(4856):1169-76
3131876
J Cell Biol. 2002 Sep 30;158(7):1263-75
12356870
Cell. 1995 Jan 27;80(2):179-85
7834738
J Mol Biol. 2005 Nov 11;353(5):1001-10
16219325
J Biol Chem. 2003 Mar 14;278(11):9536-43
12519755
J Biol Chem. 1996 Sep 6;271(36):22225-30
8703037
Biochem J. 2008 Feb 15;410(1):205-11
18039182
Trends Immunol. 2001 Sep;22(9):490-6
11525939
FEBS Lett. 1997 Feb 10;403(1):31-4
9038355
Biochem Biophys Res Commun. 2000 Jan 27;267(3):692-6
10673353
Nature. 1992 Mar 5;356(6364):71-4
1531699
J Immunol. 2006 Feb 15;176(4):2430-8
16456002
Immunol Rev. 2003 Apr;192:32-41
12670393
Immunity. 1997 Feb;6(2):155-64
9047237
J Exp Med. 1984 Nov 1;160(5):1284-99
6208306
Cell. 1994 Jan 28;76(2):263-74
8293463
J Exp Med. 1997 May 19;185(10):1877-82
9151714
Blood. 2007 Sep 15;110(6):1730-8
17537990
J Immunol. 1996 Nov 1;157(9):3769-73
8892604
EMBO J. 2002 Sep 16;21(18):4809-19
12234921
J Immunol. 2003 Jan 1;170(1):41-7
12496381