Association of P53 codon 72 polymorphism and ameloblastoma.
OBJECTIVE
To test the hypothesis that P53 codon 72 polymorphism was associated with an increased risk of developing ameloblastoma in the Thai population.


METHODS
Seventy-eight ameloblastomas and 94 healthy controls were genotyped for the P53 codon 72 by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).


RESULTS
The frequencies of the Arg/Arg, Arg/Pro and Pro/Pro genotypes were 28.72%, 50.00% and 21.28%, respectively, in the controls; and 44.87%, 51.28% and 3.85%, respectively, in ameloblastomas. Therefore, P53 Arg is an ameloblastoma-susceptible allele [OR (95% CI) = 2.06 (1.28-3.31), P = 0.002]. Sex-adjusted OR (95% CI) is 2.08 (1.29-3.34), P = 0.002; and adjusted OR by clinical type (95% CI) is 2.04 (1.34-3.13), P < 10(-3). Therefore, the increased risk associated with P53 Arg may not be influenced by either the sex of patients or clinical characteristics of the tumours. Moreover, when compared with homozygous P53 Pro, people who carried the Arg allele had a remarkably high risk of developing ameloblastoma [adjusted OR (95% CI) = 7.26 (2.34-23.41), P < 10(-3)].


CONCLUSIONS
The Arg allele of P53 gene codon 72 may increase susceptibility, and P53 may be important in the aetiology of ameloblastoma.
Kitkumthorn
N
N
Department of Oral Pathology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand. nakarinkit@hotmail.com
Yanatatsaneejit
P
P
Rabalert
J
J
Dhammawipark
C
C
Mutirangura
A
A
D000483:Alleles; D000564:Ameloblastoma / Q000235:genetics*; D001120:Arginine / Q000235:genetics; D003062:Codon / Q000235:genetics*; D003596:Cytosine; D004311:Double-Blind Method; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006147:Guanine; D006720:Homozygote; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D011392:Proline / Q000235:genetics; D012737:Sex Factors; D013785:Thailand; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D003062:Codon; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D006147:Guanine; D003596:Cytosine; D001120:Arginine; D011392:Proline