title: Synthesis of a fluorescent cationic phosphorus dendrimer and preliminary biological studies of its interaction with DNA.
abstract: The synthesis of a water-soluble phosphorus-containing dendrimer possessing a fluorophore (maleimide-type) linked to the core is described. This dendrimer is found brightly fluorescent in CH(2)Cl(2), but poorly fluorescent in water. The cytotoxicity of this compound is relatively low towards HeLa and A549 cells, and less toxic after 48 hours than after 24 hours. Association of this dendrimer with plasmid DNA (BACE-GFP) analyzed with circular dichroism (CD) indicates a possible disturbing of the helical B-type structure of DNA. The strength of this association (a "dendriplex") with BACE-GFP (also with HygEGFP) was measured by electrophoresis.
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lodz, Poland.
authors.forename: Julia
authors.initials: J
authors.lastname: Kazmierczak-Baranska
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Pietkiewicz
authors.forename: Magdalena
authors.initials: M
authors.lastname: Janicka
authors.forename: Yiqian
authors.initials: Y
authors.lastname: Wei
authors.forename: Cedric-Olivier
authors.initials: CO
authors.lastname: Turrin
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Majoral
authors.forename: Barbara
authors.initials: B
authors.lastname: Nawrot
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Caminade
mesh_terms: D002412:Cations / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D050091:Dendrimers / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D002412:Cations; D050091:Dendrimers; D009943:Organophosphorus Compounds; D004247:DNA