Neutrophil granulocyte-dependent proteolysis enhances platelet adhesion to the arterial wall under high-shear flow.
BACKGROUND
Under high shear stress platelets adhere preferentially to the adventitia layer of the arterial vessel wall in a von Willebrand factor (VWF)-dependent manner.


OBJECTIVE
The present study was undertaken in an attempt to characterize the structural background of the relative thromboresistance of the media and the impact of neutrophil leukocyte-derived proteases (matrix metalloproteinases, neutrophil elastase) on platelet adhesion in this layer of the arteries.


RESULTS
Platelet adhesion to cross-sections of the human iliac artery was monitored by indirect immunofluorescent detection of GpIIb/IIIa antigen. Exposure of the vessel wall to activated neutrophils or neutrophil-derived proteases increased platelet adhesion to the media about tenfold over the control level at 3350 s(-1) surface shear rate. In parallel with this enhanced thrombogenicity morphological changes in the media were evidenced by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The fine proteoglycan meshwork seen with Cupromeronic Blue enhancement of the SEM images was removed by the proteolytic treatment and the typical collagen fiber structure was exposed on the AFM images of the media.


CONCLUSIONS
Through their proteases activated neutrophils degrade proteoglycans, unmask VWF binding sites and thus abolish the thromboresistance of the media in human arteries.
Wohner
N
N
Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary.
Keresztes
Z
Z
Sótonyi
P
P
Szabó
L
L
Komorowicz
E
E
Machovich
R
R
Kolev
K
K
D001665:Binding Sites; D006098:Granulocytes; D006801:Humans; D007083:Iliac Artery; D009504:Neutrophils / Q000201:enzymology / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010973:Platelet Adhesiveness; D011509:Proteoglycans / Q000378:metabolism; D013314:Stress, Mechanical; D013927:Thrombosis; D017540:Tunica Media / Q000378:metabolism / Q000502:physiology*; D014841:von Willebrand Factor / Q000378:metabolism
D011509:Proteoglycans; D014841:von Willebrand Factor; D010447:Peptide Hydrolases
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