A biosensor based on zucchini (Cucurbita pepo L.) homogenate as a biorecognition layer for ascorbic acid determination.
An amperometric biosensor based on zucchini (Cucurbita pepo) tissue homogenate is presented. The zucchini tissue homogenate was crosslinked with gelatine using glutaraldehyde and fixed on a pretreated teflon membrane. The zucchini tissue contained the enzyme ascorbate oxidase and this enzyme catalyzed the oxidation of ascorbic acid in the presence of dissolved oxygen. The principle of the measurements was based on the determination of the decrease in the dissolved oxygen level. Determinations were carried out by standard curves, which were obtained by the measurement of the decrease in the oxygen level related to ascorbic acid concentration. Optimization and characterization studies of the biosensor were carried out in detail. First of all, the amounts of zucchini tissue homogenate, gelatin, and glutaraldehyde percentage were optimized. Experimental parameters such as buffer system, pH, buffer concentration, and temperature were also optimized carefully. Thermal stability, storage stability, and repeatability of the biosensor were investigated. A linear response was observed from 5x10(-6) M to 1.2x10(-3) M ascorbic acid. Finally, the results of some plant and drug samples analyzed with the presented biosensor compared with the spectrophotometric method (Tillman reagent) used as a reference.
Sezgintürk
Mustafa Kemal
MK
Namik Kemal University, Faculty of Arts and Science, Chemistry Department, Tekirdağ, Turkey. msezginturk@hotmail.com
Koca
Halit Buğra
HB
Ozben
Yavuz Selim
YS
Dinçkaya
Erhan
E
D001205:Ascorbic Acid / Q000032:analysis* / Q000378:metabolism; D001628:Beverages / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D002021:Buffers; D002138:Calibration; D028464:Cucurbita / Q000201:enzymology*; D004563:Electrochemistry; D005780:Gelatin / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D013053:Spectrophotometry; D013696:Temperature
D002021:Buffers; D004364:Pharmaceutical Preparations; D010936:Plant Extracts; D005780:Gelatin; D001205:Ascorbic Acid; D005976:Glutaral