title: Systems microscopy: an emerging strategy for the life sciences.
abstract: Dynamic cellular processes occurring in time and space are fundamental to all physiology and disease. To understand complex and dynamic cellular processes therefore demands the capacity to record and integrate quantitative multiparametric data from the four spatiotemporal dimensions within which living cells self-organize, and to subsequently use these data for the mathematical modeling of cellular systems. To this end, a raft of complementary developments in automated fluorescence microscopy, cell microarray platforms, quantitative image analysis and data mining, combined with multivariate statistics and computational modeling, now coalesce to produce a new research strategy, "systems microscopy", which facilitates systems biology analyses of living cells. Systems microscopy provides the crucial capacities to simultaneously extract and interrogate multiparametric quantitative data at resolution levels ranging from the molecular to the cellular, thereby elucidating a more comprehensive and richly integrated understanding of complex and dynamic cellular systems. The unique capacities of systems microscopy suggest that it will become a vital cornerstone of systems biology, and here we describe the current status and future prospects of this emerging field, as well as outlining some of the key challenges that remain to be overcome.
authors.affiliation: Center for Biosciences, Department of Biosciences and Nutrition, Karolinska Institutet, NOVUM, SE-141 83, Huddinge, Sweden.
authors.forename: John G
authors.initials: JG
authors.lastname: Lock
authors.forename: Staffan
authors.initials: S
authors.lastname: Strömblad
mesh_terms: D000818:Animals; D001690:Biological Science Disciplines; D019295:Computational Biology; D006801:Humans; D008856:Microscopy, Fluorescence; D049490:Systems Biology