title: Vitamin D and prostate cancer: the role of membrane initiated signaling pathways in prostate cancer progression.
abstract: 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) has been demonstrated to mediate both genomic and non-genomic responses in prostate cancer (CaP) cells. Here, we give an overview of membrane initiated 1,25(OH)2D3 signaling in prostate cancer cell progression. The presence of PDIA3 was investigated and homologous modeling of the putative PDIA3 receptor complex was conducted. Furthermore, the cellular distribution of nVDR was analyzed. We could show that both nVDR and PDIA3 are expressed in the prostate cancer cell lines investigated. The homologous modeling of PDIA3 showed that the receptor complex exists in a trimer formation, which suggests for allosteric activity. Our findings support previous reports and suggest that 1,25(OH)2D3 is an important therapeutic agent in inhibiting prostate cancer progression. Furthermore, our data show that 1,25(OH)2D3 regulate prostate cell biology via multiple pathways and targeting specific pathways for 1,25(OH)2D3 might provide more effective therapies compared to the vitamin D therapies currently clinically tested.
authors.affiliation: Systems Biology Research Centre-Biomedicine, School of Life Sciences, University of Skövde, Box 408, 541 28 Skövde, Sweden.
authors.forename: Sandra
authors.initials: S
authors.lastname: Karlsson
authors.forename: Josefin
authors.initials: J
authors.lastname: Olausson
authors.forename: Dan
authors.initials: D
authors.lastname: Lundh
authors.forename: Peter
authors.initials: P
authors.lastname: Sögård
authors.forename: Abul
authors.initials: A
authors.lastname: Mandal
authors.forename: Kjell-Ove
authors.initials: KO
authors.lastname: Holmström
authors.forename: Anette
authors.initials: A
authors.lastname: Stahel
authors.forename: Jenny
authors.initials: J
authors.lastname: Bengtsson
authors.forename: Dennis
authors.initials: D
authors.lastname: Larsson
mesh_terms: D000495:Allosteric Site; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D018450:Disease Progression; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008958:Models, Molecular; D008962:Models, Theoretical; D011471:Prostatic Neoplasms / Q000378:metabolism*; D011487:Protein Conformation; D018167:Receptors, Calcitriol / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014807:Vitamin D / Q000378:metabolism*
chemical_list: D018167:Receptors, Calcitriol; D014807:Vitamin D