title: Spatial and temporal changes of osteopontin in oxygen-glucose-deprived hippocampal slice cultures.
abstract: We have examined the temporal changes and cellular localization of osteopontin (OPN) mRNA and protein in organotypic hippocampal slice cultures subjected to ischemia-like oxygen-glucose deprivation (OGD). The sequential induction pattern response consisted of neuronal and microglial OPN upregulation, followed by a later extended phase of expression in reactive astrocytes. OPN immunoreactivity after OGD matched the mRNA induction patterns. Activated microglia revealed OPN staining in focal deposits, whereas neurons and reactive astrocytes showed perinuclear staining with a punctate cytosolic pattern of OPN, typical of secreted proteins. These data demonstrated that the temporal and cellular patterns of OPN induction in reactive glial cells in this in vitro model closely correlated with that in the in vivo model, suggesting that OPN has a multifunctional role in the pathogenesis of ischemic injury.
authors.affiliation: Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Lee
authors.forename: Jeong-Sun
authors.initials: JS
authors.lastname: Choi
authors.forename: Jae-Youn
authors.initials: JY
authors.lastname: Choi
authors.forename: Yoo-Jin
authors.initials: YJ
authors.lastname: Shin
authors.forename: Hou
authors.initials: H
authors.lastname: Yun
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Cha
authors.forename: Myung-Hoon
authors.initials: MH
authors.lastname: Chun
authors.forename: Mun-Yong
authors.initials: MY
authors.lastname: Lee
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002135:Calcium-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000172:deficiency*; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D000860:Hypoxia / Q000473:pathology*; D008840:Microfilament Proteins; D009474:Neurons / Q000378:metabolism; D009924:Organ Culture Techniques; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; D053495:Osteopontin; D010751:Phosphopyruvate Hydratase; D005947:Glucose