[Abnormal expression of microRNAs in the hippocampus of Ts65Dn mice].
OBJECTIVE
To identify the differentially expressed microRNAs in the hippocampus of Down Syndrome model mouse Ts65Dn.


METHODS
Low molecular weight RNA from hippocampus were tailed and reverse transcribed by extended RT-primer. MicroRNAs primers were arrayed on plates according to the Tm of each primer. PCR were carried out at different annealing temperatures using a gradient real-time PCR instrument. The relative expression level of each microRNAs was calculated using the most stable reference gene as normalizer which was selected by geNorm and Normfinder software.


RESULTS
miR-27a was identified as the most stable reference gene by geNorm and Normfinder software. Among the 52 microRNAs detected by real-time PCR array, miR-33 and miR-19a were significantly down-regulated, whereas miR-130 were significantly up-regulated in the hippocampus of Ts65Dn mice as compared with the euploid control mice. The expression of miR-802, which was the trisomy chromosome 16 derived microRNAs, was very low in hippocampus with no difference between the two groups.


CONCLUSIONS
MicroRNAs are dysregulated in the hippocampus of Ts65Dn mice, which may contribute to the reduced neurogenesis of Down syndrome.
Zhang
Yu-jun
YJ
Institute of Clinical Molecular Biology, Peking University People's Hospital, Beijing 100044, China.
He
Xiang-jun
XJ
Liu
Yu-jing
YJ
Zhang
Qi
Q
D000818:Animals; D004314:Down Syndrome / Q000235:genetics*; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D008818:Mice, Neurologic Mutants; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology
C582652:MIRN130 microRNA, mouse; D035683:MicroRNAs; D012333:RNA, Messenger