title: Proteomic analysis of the effects of ABA treatments on ripening Vitis vinifera berries.
abstract: The control of ripening of the non-climacteric grapevine fruit is still a matter of debate, but several lines of evidence point to an important role for the hormone abscisic acid (ABA). The effects of ABA treatments on Cabernet Sauvignon berries before and at véraison were studied using a 2-DE proteomic approach. Proteins from whole deseeded berries (before véraison) and berry flesh and skin (at véraison) treated with 0.76 mM ABA and collected 24 h after treatment were separated and analysed. A total of 60 protein spots showed significant variations between treated and control berries, and 40 proteins, mainly related to general metabolism and cell defence, were identified by LC MS/MS. Our results show that ABA acts mainly through the regulation of mostly the same proteins which are involved in the ripening process, and that several of these changes share common elements with the ABA-induced responses in vegetative tissues.
authors.affiliation: Università di Torino, Dipartimento Colture arboree, via Leonardo da Vinci 44, 10095 Grugliasco (TO), Italy. marzia.giribaldi@ispa.cnr.it
authors.forename: Marzia
authors.initials: M
authors.lastname: Giribaldi
authors.forename: Laurence
authors.initials: L
authors.lastname: Gény
authors.forename: Serge
authors.initials: S
authors.lastname: Delrot
authors.forename: Andrea
authors.initials: A
authors.lastname: Schubert
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005638:Fruit / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D027843:Vitis / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D000040:Abscisic Acid
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