[Effects of 17β-estradiol on proliferation and migration of MCF-7 cell by regulating expression of claudin-6].
OBJECTIVE
To explore the role of estrogen in the regulation of the expression of claudin-6 and biological behavior in MCF-7 cells.


METHODS
RT-PCR and immunocytochemistry were conducted to analyze the expression and localization of claudin-6 in MCF-7 cells treated with 17β-estradiol. CCK-8 kit assay and Scratch Test were conducted to analyze the capability of proliferation and migration of 17β-estradiol treated MCF-7 cells.


RESULTS
RT-PCR analysis and immunocytochemistry showed that 17β-estradiol induced a concentration-and time-dependent effect on claudin-6. At 5 nmol/L and at 24 h, 17β-estradiol treatment led to an increased level of claudin-6, which was located in the membrane of MCF-7 cells. CCK-8 analysis showed a significant decrease in the capability of proliferation of MCF-7 cells compared with the control group (P < 0.05). Cells Scratch Test showed decreased migration capability of MCF-7 cells compared with the control group (P > 0.05).


CONCLUSIONS
17β-E2 might regulate the expression of claudin-6 and inhibit the proliferation and migration of MCF-7 cells. The inhibitory effects of 17β-E2 on growth and migration of MCF-7 cells may be mediated by claudin-6 expression regulation.
Liu
Ya-fang
YF
Jilin University, Changchun, China.
Wu
Qiong
Q
Xu
Xiao-ming
XM
Ren
Yue
Y
Yu
Li-na
LN
Quan
Cheng-shi
CS
Li
Yu-lin
YL
D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D057167:Claudins; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*
D057167:Claudins; D008565:Membrane Proteins; D004958:Estradiol; C122101:claudin 6