title: Flavonoids and antioxidant activity of Rosa agrestis leaves.
abstract: In this work we report the isolation and characterisation of seven flavonoids, the levels of total phenolics, flavonoids and proanthocyanidins, and the antioxidant activity of the leaf extract of Rosa agrestis Savi (Rosaceae). The results showed that the R. agrestis leaf extract exhibited significant antioxidative activity as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) (EC(50) = 47.4 microg mL(-1)), inhibited both beta-carotene bleaching and deoxyribose degradation, quenched a chemically generated superoxide anion in vitro and showed high ferrous ion chelating activity. Reactivity towards 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical cation and ferric-reducing antioxidant power (FRAP) values were equivalent to 2.30 mM L(-1) Trolox, the water soluble alpha-tocopherol analogue, and 1.91 mM L(-1) Fe(2+), respectively. The high antioxidant activity of the extract appeared to be attributed to its high content of total phenolics, flavonoids and proanthocyanidins. The flavonoids isolated from R. agrestis leaves were diosmetin, kaempherol, quercetin, kaempherol 3-glucoside (astragalin), quercetin 3-rhamnoside (quercitrin), quercetin 3-xyloside and quercetin 3-galactoside (hyperoside). Diosmetin (5,7,3'-trihydroxy-4'-methoxyflavone) was isolated for the first time from Rosa species.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Marmara University, Istanbul, 34668 Haydarpasa, Turkey.
authors.forename: Leyla
authors.initials: L
authors.lastname: Bitis
authors.forename: Sukran
authors.initials: S
authors.lastname: Kultur
authors.forename: Gulay
authors.initials: G
authors.lastname: Melikoglu
authors.forename: Nurten
authors.initials: N
authors.lastname: Ozsoy
authors.forename: Ayse
authors.initials: A
authors.lastname: Can
mesh_terms: D001713:Biphenyl Compounds; D005290:Ferric Compounds; D005419:Flavonoids / Q000032:analysis*; D016166:Free Radical Scavengers / Q000032:analysis*; D017665:Hydroxyl Radical; D007502:Iron Chelating Agents / Q000032:analysis*; D019787:Linoleic Acid; D010636:Phenols / Q000032:analysis; D010851:Picrates; D018515:Plant Leaves / Q000737:chemistry; D044945:Proanthocyanidins / Q000032:analysis; D027826:Rosa / Q000737:chemistry*; D013481:Superoxides; D019207:beta Carotene
chemical_list: D001713:Biphenyl Compounds; D005290:Ferric Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D010636:Phenols; D010851:Picrates; D044945:Proanthocyanidins; D019207:beta Carotene; D013481:Superoxides; D017665:Hydroxyl Radical; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl