Crystallization and preliminary X-ray study of the D-altritol oligonucleotide GTGTACAC.
In altritol nucleic acids (ANAs), the natural five-membered ribose ring of RNA is replaced by the six-membered D-altritol ring. ANAs are good candidates to act as siRNAs in the RNA-interference pathway. Crystals of the fully modified altritol self-complementary octamer GTGTACAC were grown by the hanging-drop vapour-diffusion technique at 289 K. Diffraction data were recorded on SLS beamline X06DA and processed to 3.0 A resolution. The crystals belonged to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = 25.05, c = 117.58 A.
Ovaere
Margriet
M
Department of Chemistry, Biomolecular Architecture and BioMacS, Katholieke Universiteit Leuven, Belgium.
Van Aerschot
Arthur
A
Abramov
Mikhail
M
Herdewijn
Piet
P
Van Meervelt
Luc
L
D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D009841:Oligonucleotides / Q000737:chemistry*; D013402:Sugar Alcohols / Q000737:chemistry*
D009841:Oligonucleotides; D013402:Sugar Alcohols; C042890:altritol
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