Artificial receptor-functionalized nanoshell: facile preparation, fast separation and specific protein recognition.
This work combined molecular imprinting technology with superparamagnetic nanospheres as the core to prepare artificial receptor-functionalized magnetic nanoparticles for separation of homologous proteins. Using dopamine as a functional monomer, novel surface protein-imprinted superparamagnetic polydopamine (PDA) core-shell nanoparticles were successfully prepared in physiological conditions, which could maintain the natural structure of a protein template and achieved the development of molecularly imprinted polymers (MIPs) from one dimension to zero dimension for efficient recognition towards large biomolecules. The resultant nanoparticles could be used for convenient magnetic separation of homologous proteins with high specificity. The nanoparticles possessed good monodispersibility, uniform surface morphology and high saturation magnetization value. The bound amounts of template proteins measured by both indirect and direct methods were in good agreement. The maximum number of imprinted cavities on the surface of the bovine hemoglobin (Hb)-imprinted nanoshell was 2.21 x 10(18) g( - 1), which well matched their maximum binding capacity toward bovine Hb. Both the simple method for preparation of MIPs and the magnetic nanospheres showed good application potential in fast separation, effective concentration and selective biosensing of large protein molecules.
Ouyang
Ruizhuo
R
Key Laboratory of Analytical Chemistry for Life Science (Education Ministry of China), Department of Chemistry, Nanjing University, Nanjing 210093, People's Republic of China.
Lei
Jianping
J
Ju
Huangxian
H
D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D004574:Electromagnetic Fields; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009584:Nitrogen / Q000378:metabolism; D009994:Osmolar Concentration; D056951:Photoelectron Spectroscopy; D011108:Polymers; D016062:Porosity; D011485:Protein Binding; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared
D006454:Hemoglobins; D011108:Polymers; D009584:Nitrogen