The effects of environmental salinity on trunk kidney proteome of juvenile ayu (Plecoglossus altivelis).
As the life cycle of ayu spans river, brackish and seawater environments, it would be a suitable fish model for studying the responses to salinity changes in aquatic animals. We investigated the effect of salinity on trunk kidney proteome in ayu (Plecoglossus altivelis) using two-dimensional gel electrophoresis and mass spectrometry. The proteins involved in the process of energy metabolism, biosynthesis, DNA methylation and cell differentiation were mainly affected, and 10 significantly changed proteins were identified. Our result showed that isocitrate dehydrogenase (ICD), pyruvate dehydrogenase (E1), O-glycosyl hydrolase, mitochondrial precursor of ATP synthase subunit beta, mitochondrial ferrtin (MtF), retinol binding protein (RBP) were down-regulated, whereas aldehyde dehydrogenase, cytokeratin 1, S-adenosylhomocysteine hydrolase, Cys-Met metabolism PLP-dependent enzyme were up-regulated when ayu transferred from freshwater to brackish water. Partial coding sequences of E1, ICD, MtF and RBP genes were determined, and the effects of salinity on their mRNA expression in ayu trunk kidney were tested by real-time PCR subsequently. Their possible direct or indirect roles in the adaptation of ayu to salinity are discussed.
Chen
Jiong
J
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo city 315211, Zhejiang province, People's Republic of China. Electronic address: jchen1975@163.com.
Wu
Hai Q
HQ
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo city 315211, Zhejiang province, People's Republic of China.
Shi
Yu H
YH
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo city 315211, Zhejiang province, People's Republic of China.
Li
Chang H
CH
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo city 315211, Zhejiang province, People's Republic of China.
Li
Ming Y
MY
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo city 315211, Zhejiang province, People's Republic of China.
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D029941:Fish Proteins / Q000032:analysis / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism*; D036441:Osmeriformes; D016133:Polymerase Chain Reaction; D020543:Proteome / Q000187:drug effects; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D029941:Fish Proteins; D020543:Proteome; D012965:Sodium Chloride