Rnf8 deficiency impairs class switch recombination, spermatogenesis, and genomic integrity and predisposes for cancer.
Signaling and repair of DNA double-strand breaks (DSBs) are critical for preventing immunodeficiency and cancer. These DNA breaks result from exogenous and endogenous DNA insults but are also programmed to occur during physiological processes such as meiosis and immunoglobulin heavy chain (IgH) class switch recombination (CSR). Recent studies reported that the E3 ligase RNF8 plays important roles in propagating DNA DSB signals and thereby facilitating the recruitment of various DNA damage response proteins, such as 53BP1 and BRCA1, to sites of damage. Using mouse models for Rnf8 mutation, we report that Rnf8 deficiency leads to impaired spermatogenesis and increased sensitivity to ionizing radiation both in vitro and in vivo. We also demonstrate the existence of alternative Rnf8-independent mechanisms that respond to irradiation and accounts for the partial recruitment of 53bp1 to sites of DNA damage in activated Rnf8(-/-) B cells. Remarkably, IgH CSR is impaired in a gene dose-dependent manner in Rnf8 mutant mice, revealing that these mice are immunodeficient. In addition, Rnf8(-/-) mice exhibit increased genomic instability and elevated risks for tumorigenesis indicating that Rnf8 is a novel tumor suppressor. These data unravel the in vivo pleiotropic effects of Rnf8.
Li
Li
L
Department of Medical Biophysics, University of Toronto and Ontario Cancer Institute, University Health Network, Toronto, Ontario M5G 2M9, Canada.
Halaby
Marie-Jo
MJ
Hakem
Anne
A
Cardoso
Renato
R
El Ghamrasni
Samah
S
Harding
Shane
S
Chan
Norman
N
Bristow
Robert
R
Sanchez
Otto
O
Durocher
Daniel
D
Hakem
Razqallah
R
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D004249:DNA Damage / Q000235:genetics; D020022:Genetic Predisposition to Disease; D042822:Genomic Instability / Q000235:genetics; D006128:Growth / Q000235:genetics / Q000276:immunology; D017578:Immunoglobulin Class Switching / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D009369:Neoplasms / Q000235:genetics* / Q000276:immunology; D013091:Spermatogenesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D044767:Ubiquitin-Protein Ligases / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
C549817:Rnf8 protein, mouse; D044767:Ubiquitin-Protein Ligases
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