The role of the transcript elongation factors FACT and HUB1 in leaf growth and the induction of flowering.
In the cell nucleus, the packaging of the DNA into chromatin represses transcription by restricting the access of transcriptional regulators to their binding sites and inhibiting the progression of RNA polymerases during transcript elongation. To efficiently transcribe genes in the context of chromatin, eukaryotes have a variety of transcript elongation factors promoting transcription in vivo. The facilitates chromatin transcription (FACT) complex consisting of the SSRP1 and SPT16 proteins, is a histone chaperone that assists transcription by destabilising nucleosomes in the path of RNA polymerases. In a recent study, we report that Arabidopsis FACT is critically involved in different aspects of development including leaf growth and the transition to flowering. Moreover, FACT was found to interact genetically with HUB1 that mono-ubiquitinates histone H2B. Depending on the underlying process that is regulated by the two complexes, there appear to be different levels of interaction.
Van Lijsebettens
Mieke
M
Department of Plant Systems Biology, Flanders Institute for Biotechnology, VIB, Ghent, Belgium.
Grasser
Klaus D
KD
Mol Cell. 2009 Aug 14;35(3):365-76
19683499
Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11812-7
11562463
Mol Biosyst. 2008 Nov;4(11):1085-93
18931784
J Exp Bot. 2008;59(3):653-66
18296430
Plant Cell. 2007 Feb;19(2):417-32
17329565
Plant J. 2009 Feb;57(3):522-33
18980658
Mol Plant. 2009 Jul;2(4):554-564
19825638
Plant Cell. 2001 Mar;13(3):599-612
11251099
Development. 2002 Feb;129(4):957-64
11861478
Trends Genet. 2009 Aug;25(8):368-76
19665253
Biochem Cell Biol. 2004 Aug;82(4):419-27
15284894
Plant J. 2010 Feb;61(4):686-97
19947984
Mol Cell Biol. 2003 Aug;23(15):5301-7
12861016
J Biol Chem. 2003 Apr 11;278(15):12710-5
12571244
Semin Cell Dev Biol. 2008 Dec;19(6):560-73
18708152
J Biol Chem. 2006 Aug 18;281(33):23297-301
16766522
Plant J. 2009 Jan;57(2):279-88
18798874
Cell. 1998 Jan 9;92(1):105-16
9489704
Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1678-83
20080602
Plant Physiol. 2006 Jul;141(3):1078-88
16679423
Plant Cell. 2008 Oct;20(10):2586-602
18849490
Trends Plant Sci. 2005 Oct;10(10):484-90
16150628
Plant J. 2000 Aug;23(3):395-405
10929132
New Phytol. 2006;169(3):623-35
16411964
Science. 2003 Aug 22;301(5636):1090-3
12934006
Plant J. 2004 Dec;40(5):660-71
15546350
Plant Physiol. 2009 Nov;151(3):1476-85
19726574
Biochemistry. 2001 Jul 3;40(26):7860-7
11425313