title: Investigation on the interaction of prulifloxacin with human serum albumin: a spectroscopic analysis.
abstract: The interaction between prulifloxacin (PUFX) and human serum albumin (HSA) was investigated under simulated physiologic conditions with fluorescence spectra. The fluorescence quenching process of HSA may be mainly governed by a static quenching mechanism. The apparent binding constant K(b) between PUFX and HSA at different temperatures were 2.08+/-1.04, 2.74+/-0.50, and 4.98+/-1.61x10(8) l/mol. The thermodynamic parameters, with a negative value of DeltaG(0), revealed that the binding is a spontaneous process. A binding distance R of 1.19 nm between donor and acceptor was obtained from the Fŏrster energy transfer theory.
authors.affiliation: Department of Chemistry, Huaibei Coal Normal College, Huaibei Occupational Disease Prevention and Cure Hospital, Huaibei, Anhui Province, P R China
authors.forename: Ya-Bei
authors.initials: YB
authors.lastname: Huang
authors.forename: Shi-Jun
authors.initials: SJ
authors.lastname: Wang
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Zi
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yu
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Gao
authors.forename: Ying-Cai
authors.initials: YC
authors.lastname: Tang
authors.forename: Da-Ming
authors.initials: DM
authors.lastname: Zhang
mesh_terms: D001665:Binding Sites; D004148:Dioxolanes / Q000737:chemistry / Q000378:metabolism*; D024841:Fluoroquinolones / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D010879:Piperazines / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D004148:Dioxolanes; D024841:Fluoroquinolones; D010879:Piperazines; D012709:Serum Albumin; C074191:prulifloxacin