title: Upregulation of ANP and NPR-C mRNA in the kidney and heart of eNOS knockout mice.
abstract: OBJECTIVE
  The aim of the present studywas to examine the question of whether the atrial natriuretic peptide (ANP) system is altered by endothelial nitric-oxide synthase (eNOS).
  
  
  METHODS
  Male eNOS-deficient mice (eNOS-/-) and wild type control mice (eNOS+/+, C57B1/6J) were used. Blood pressure was measured in anesthetized mice by tail cuff plethysmography and renal function was measured. Expression of ANP, natriuretic peptide receptor (NPR)-A, NPR-C, and tonicity-responsive enhancer binding protein (TonEBP) mRNA was determined by real-time PCR. Localization of (125)I-ANP binding sites was measured using in vitro autoradiography.
  
  
  RESULTS
  In eNOS-/- mice, systolic blood pressure increased and left ventricular hypertrophy was observed. Urine volume and osmolarity did not change. Expression of ANP markedly increased in the heart and kidney of eNOS-/- mice. Expression of NPR-A and NPR-C increased in the heart and tended to increase in the kidney of eNOS-/- mice. In the renal medulla in particular, increased expression of NPR-C was more prominent. Expression of TonEBP mRNA was markedly decreased in the renal medulla, but not in the renal cortex. Maximum binding capacity (B(max)) of ANP and C-ANP increased in the renal medulla in eNOS-/- mice.
  
  
  CONCLUSIONS
  These results suggest that the eNOS-NO system may be partly involved in regulation of ANP, NPR-A, -C, and TonEBP mRNA expression in the kidney.
authors.affiliation: Department of Physiology, Diabetic Research Center, Chonbuk National University Medical School, Jeonju 561-180, Republic of Korea.
authors.forename: Kuichang
authors.initials: K
authors.lastname: Yuan
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Young-Bin
authors.initials: YB
authors.lastname: Oh
authors.forename: Jiahua
authors.initials: J
authors.lastname: Yu
authors.forename: Amin
authors.initials: A
authors.lastname: Shah
authors.forename: Byung Hyun
authors.initials: BH
authors.lastname: Park
authors.forename: Suhn Hee
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009206:Myocardium / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C492200:Nfat5 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors; D009320:Atrial Natriuretic Factor; D052250:Nitric Oxide Synthase Type III; D017461:Receptors, Atrial Natriuretic Factor; C073616:atrial natriuretic factor receptor C