Atlantic cod trypsin-catalyzed peptide synthesis with inverse substrates as acyl donor components.
Atlantic cod trypsin-catalyzed peptide synthesis has been studied by using p-amidino- and p-guanidinophenyl esters of N-(tert-butyloxycarbonyl)amino acid as acyl donor components. The reaction temperature was optimized at 0 degrees C. The method was shown to be successful as effectively for synthesizing the peptide and useful for preparing dipeptide between D-amino acid with D-amino acid and beta-amino acid with beta-amino acid, respectively. The enzymatic hydrolysis of the resulting products was negligible.
Fuchise
Tomoyoshi
T
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, Japan.
Kishimura
Hideki
H
Yang
Zhi-hong
ZH
Kojoma
Mareshige
M
Toyota
Eiko
E
Sekizaki
Haruo
H
D000818:Animals; D002384:Catalysis; D048389:Gadus morhua / Q000378:metabolism*; D010452:Peptide Biosynthesis; D012474:Salmon / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism*
D014357:Trypsin