title: Atlantic cod trypsin-catalyzed peptide synthesis with inverse substrates as acyl donor components.
abstract: Atlantic cod trypsin-catalyzed peptide synthesis has been studied by using p-amidino- and p-guanidinophenyl esters of N-(tert-butyloxycarbonyl)amino acid as acyl donor components. The reaction temperature was optimized at 0 degrees C. The method was shown to be successful as effectively for synthesizing the peptide and useful for preparing dipeptide between D-amino acid with D-amino acid and beta-amino acid with beta-amino acid, respectively. The enzymatic hydrolysis of the resulting products was negligible.
authors.affiliation: Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, Japan.
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Fuchise
authors.forename: Hideki
authors.initials: H
authors.lastname: Kishimura
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Yang
authors.forename: Mareshige
authors.initials: M
authors.lastname: Kojoma
authors.forename: Eiko
authors.initials: E
authors.lastname: Toyota
authors.forename: Haruo
authors.initials: H
authors.lastname: Sekizaki
mesh_terms: D000818:Animals; D002384:Catalysis; D048389:Gadus morhua / Q000378:metabolism*; D010452:Peptide Biosynthesis; D012474:Salmon / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism*
chemical_list: D014357:Trypsin