1H, 13C, 15N assignments of the dimeric regulatory subunit (ilvN) of the E. coli AHAS I.
Acetohydroxyacid synthase (AHAS) is an enzyme involved in the biosynthesis of the branched chain amino acids viz, valine, leucine and isoleucine. The activity of this enzyme is regulated through feedback inhibition by the end products of the pathway. Here we report the backbone and side-chain assignments of ilvN, the 22 kDa dimeric regulatory subunit of E. coli AHAS isoenzyme I, in the valine bound form. Detailed analysis of the structure of ilvN and its interactions with the catalytic subunit of E. coli AHAS I will help in understanding the mechanism of activation and regulation of the branched chain amino acid biosynthesis.
Karanth
N Megha
NM
Lab No. 207, Molecular Biophysics Unit, Indian Institute of Science, Bangalore, 560012, India.
Sarma
Siddhartha P
SP
D000095:Acetolactate Synthase / Q000737:chemistry*; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D006859:Hydrogen; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D055503:Protein Multimerization; D021122:Protein Subunits / Q000737:chemistry*
D002247:Carbon Isotopes; D029968:Escherichia coli Proteins; D009587:Nitrogen Isotopes; D021122:Protein Subunits; D006859:Hydrogen; D000095:Acetolactate Synthase; C510093:IlvN protein, E coli