Phagocytic activity of macrophages against liposomes with conjugates of oxidized dextrans and isonicotinic acid hydrazide during modeling of phagocytosis disturbances in vitro.
We studied phagocytic activity of macrophages against molecular-liposome hybrid compositions consisting of liposomes (diameter 200-450 nm) containing oxidized dextrans with a molecular weight of 35 or 60 kDa conjugated with the basic antituberculosis preparation isonicotinic acid hydrazide (dextrazides) during modeling of various disturbances of endocytosis function of phagocytic cells in vitro. Preincubation of macrophages with trypsin, colchicine, or sodium azide did not change the parameters of adhesion of molecular-liposome hybrid compositions to macrophages. It was found that preincubation of cells with colchicine or sodium azide reduced parameters of phagocytosis of the molecular-liposome hybrid compositions; this reduction did not depend on the molecular weight of dextrans entering the composition of the molecular-liposome hybrid compositions.
Arkhipov
S A
SA
Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
Shkurupy
V A
VA
Troitsky
A V
AV
Luzgina
N G
NG
Ufimceva
E G
EG
Zaikovskaja
M V
MV
Iljine
D A
DA
Akhramenko
E S
ES
Gulyaeva
E P
EP
Bistrova
T N
TN
D000818:Animals; D003911:Dextrans / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism; D054872:Fatty Acid Synthesis Inhibitors / Q000737:chemistry; D007538:Isoniazid / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D010587:Phagocytosis / Q000502:physiology*
D003911:Dextrans; D004337:Drug Carriers; D054872:Fatty Acid Synthesis Inhibitors; D008081:Liposomes; D007538:Isoniazid