title: [Differential gene expressions of steroid- and catecholamine-synthesizing enzymes in adrenal gland of rats with different diseases but the same syndromes].
abstract: OBJECTIVE
  To study the gene expression characteristics of steroid- and catecholamine-synthesizing enzymes in adrenal gland of spontaneously hypertensive rats (SHRs), Goto-Kakizaki (GK) rats and normal Wistar rats with the same traditional Chinese medicine syndromes.
  
  
  METHODS
  Sixteen-week-old Wistar rats, SHRs and GK rats were used. By the quantitative four diagnosis and syndrome differentiation methods and GeneChip Mouse Exon 1.0 ST Array, we observed adrenal gland gene expressions in normal Wistar rats, qi deficiency Wistar rats, SHRs with qi deficiency and qi excess, GK rats with qi deficiency and qi excess. Differentially expressed genes of steroid- and catecholamine-synthesizing enzymes and their regulatory factors were analyzed.
  
  
  RESULTS
  Thirty-one genes were differentially expressed among all syndromes. Hsd3b6 was down-regulated significantly 6.0-fold in GK rats with qi deficiency syndrome and qi excess syndrome, and Cyp11b2 was up-regulated 1.5 times in GK rats with qi deficiency syndrome. Por, Hsd11b2, and Nr2f6 were up-regulated in all syndromes, and Cyp2c23, Cyp4a3, Cyp4a8 and Cyp2e1 were down-regulated. However, Srd5a1 and Nr4a1 were up-regulated only in GK rats, and Lss was down-regulated only in SHRs. Th was up-regulated 1.5 times in SHRs with qi deficiency syndrome, GK rats with qi deficiency syndrome and GK rats with qi excess syndrome. Ddc was up-regulated 1.5 times in GK rats with qi excess syndrome. Dbh was up-regulated 3.0 times in GK rats with qi deficiency syndrome and qi excess syndrome. However, Comt was down-regulated 1.5 times in GK rats with qi deficiency syndrome and qi excess syndrome, and Mao was up-regulated 1.5 times in SHRs with qi deficiency syndrome and qi excess syndrome.
  
  
  CONCLUSIONS
  Some genes associated with steroid- and catecholamine-synthesizing pathways were differentially expressed in SHRs and GK rats, and the differentially expressed genes may be related to the development of traditional Chinese medicine syndromes.
authors.affiliation: School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Pan
authors.forename: Zhao-qin
authors.initials: ZQ
authors.lastname: Fang
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Lu
authors.forename: Xiao-mei
authors.initials: XM
authors.lastname: Liu
authors.forename: Dong-yuan
authors.initials: DY
authors.lastname: Guan
authors.forename: Chao
authors.initials: C
authors.lastname: Liang
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000096:biosynthesis*; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000628:therapy; D005786:Gene Expression Regulation; D006973:Hypertension / Q000235:genetics* / Q000628:therapy; D008297:Male; D008516:Medicine, Chinese Traditional; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D013256:Steroids / Q000096:biosynthesis*
chemical_list: D002395:Catecholamines; D013256:Steroids