title: Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL).
abstract: The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for LFucalpha1--> and related complexes and for high density Manalpha1--> complexes present in polymannosylated target macromolecules.
authors.affiliation: Institute of Molecular and Cellular Biology, College of Medicine, Chang-Gung University, Kwei-san, Tao-yuan, Taiwan. amwu@mail.cgu.edu.tw
authors.forename: Albert M
authors.initials: AM
authors.lastname: Wu
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Gong
authors.forename: Chia-Chen
authors.initials: CC
authors.lastname: Li
authors.forename: Nechama
authors.initials: N
authors.lastname: Gilboa-Garber
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism*; D002241:Carbohydrates / Q000235:genetics*; D037102:Lectins / Q000235:genetics* / Q000378:metabolism*; D008024:Ligands; D008968:Molecular Conformation; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D002241:Carbohydrates; D037102:Lectins; D008024:Ligands; C038692:adhesin, Pseudomonas