Oligodendroglioma cell lines containing t(1;19)(q10;p10).
Investigating the biology of oligodendroglioma and its characteristic combined deletion of chromosomal arms 1p and 19q, mediated by an unbalanced translocation, t(1;19)(q10;p10), has been hampered by the lack of cell lines that harbor these traits. We grew cells from 2 anaplastic oligodendrogliomas in serum-free conditions. Serial propagation and expansion led to the establishment of permanent cell lines that maintained the genetic signature of the parent oligodendrogliomas and displayed features of brain tumor stem cells in vitro. One line was established from a treatment-naïve tumor and the other from a temozolomide resistant recurrent tumor. These lines may be important tools for understanding the biology of oligodendrogliomas and the function of their defining genetic traits.
Kelly
John J P
JJ
Faculty of Medicine, Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada.
Blough
Michael D
MD
Stechishin
Owen D M
OD
Chan
Jennifer A W
JA
Beauchamp
Desiree
D
Perizzolo
Marco
M
Demetrick
Doug J
DJ
Steele
Lisa
L
Auer
Roland N
RN
Hader
Walter J
WJ
Westgate
Morgan
M
Parney
Ian F
IF
Jenkins
Robert
R
Cairncross
J Gregory
JG
Weiss
Samuel
S
D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D006801:Humans; D009837:Oligodendroglioma / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic / Q000235:genetics
J Clin Oncol. 2006 Jun 20;24(18):2707-14
16782910
Cell. 1990 Feb 23;60(4):585-95
1689217
J Clin Oncol. 2006 Oct 10;24(29):4746-53
16954518
Science. 1992 Mar 27;255(5052):1707-10
1553558
Front Biosci. 2003 Jan 01;8:a1-9
12456321
Nature. 2004 Nov 18;432(7015):396-401
15549107
Am J Pathol. 1999 Aug;155(2):375-86
10433931
Dev Biol. 1996 Apr 10;175(1):1-13
8608856
Nat Rev Cancer. 2006 Jun;6(6):425-36
16723989
Mol Chem Neuropathol. 1992 Jun;16(3):303-17
1329795
J Neuropathol Exp Neurol. 2006 Oct;65(10):988-94
17021403
Cancer Res. 2004 Oct 1;64(19):7011-21
15466194
N Engl J Med. 2009 Feb 19;360(8):765-73
19228619
Cancer Res. 2006 Oct 15;66(20):9852-61
17047046
Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14720-5
11121071
Cancer Cell. 2006 May;9(5):391-403
16697959
Cancer Res. 2003 Sep 15;63(18):5821-8
14522905
J Natl Cancer Inst. 1998 Oct 7;90(19):1473-9
9776413
Science. 2008 Sep 26;321(5897):1807-12
18772396
N Engl J Med. 2005 Mar 10;352(10):997-1003
15758010
Acta Neuropathol. 1977 May 16;38(2):129-35
195435
Stem Cells. 2009 Aug;27(8):1722-33
19544433