title: Co-culture of primary rat hepatocytes with rat liver epithelial cells enhances interleukin-6-induced acute-phase protein response.
abstract: Three different primary rat hepatocyte culture methods were compared for their ability to allow the secretion of fibrinogen and albumin under basal and IL-6-stimulated conditions. These culture methods comprised the co-culture of hepatocytes with rat liver epithelial cells (CC-RLEC), a collagen type I sandwich culture (SW) and a conventional primary hepatocyte monolayer culture (ML). Basal albumin secretion was most stable over time in SW. Fibrinogen secretion was induced by IL-6 in all cell culture models. Compared with ML, CC-RLEC showed an almost three-fold higher fibrinogen secretion under both control and IL-6-stimulated conditions. Induction of fibrinogen release by IL-6 was lowest in SW. Albumin secretion was decreased after IL-6 stimulation in both ML and CC-RLEC. Thus, cells growing under the various primary hepatocyte cell culture techniques react differently to IL-6 stimulation with regard to acute-phase protein secretion. CC-RLEC is the preferred method for studying cytokine-mediated induction of acute-phase proteins, because of the pronounced stimulation of fibrinogen secretion upon IL-6 exposure under these conditions.
authors.affiliation: Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: Stephan J A C
authors.initials: SJ
authors.lastname: Peters
authors.forename: Tamara
authors.initials: T
authors.lastname: Vanhaecke
authors.forename: Peggy
authors.initials: P
authors.lastname: Papeleu
authors.forename: Vera
authors.initials: V
authors.lastname: Rogiers
authors.forename: Henk P
authors.initials: HP
authors.lastname: Haagsman
authors.forename: Klaske
authors.initials: K
authors.lastname: van Norren
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism*; D000210:Acute-Phase Reaction / Q000473:pathology*; D000418:Albumins / Q000378:metabolism; D000818:Animals; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology*; D005340:Fibrinogen / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D015850:Interleukin-6 / Q000494:pharmacology*; D008099:Liver / Q000166:cytology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D000209:Acute-Phase Proteins; D000418:Albumins; D015850:Interleukin-6; D005340:Fibrinogen
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