Phylogenetic analysis of complete rRNA gene sequence of Nosema philosamiae isolated from the lepidopteran Philosamia cynthia ricini.
ABSTRACT. The microsporidian Nosema philosamiae is a pathogen that infects the eri-silkworm Philosamia cynthia ricini. The complete sequence of rRNA gene (4,314 bp) was obtained by polymerase chain reaction amplification with specific primers and sequencing. The sequence analysis showed that the organization of the rRNA of N. philosamiae was similar to the pattern of Nosema bombycis. Phylogenetic analysis of rRNA gene sequences revealed that N. philosamiae had a close relationship with other Nosema species, confirming that N. philosamiae is correctly assigned to the genus Nosema.
Zhu
Feng
F
Jiangsu University of Science and Technology, Zhenjiang 212018, China.
Shen
Zhongyuan
Z
Xu
Xiaofang
X
Tao
Hengping
H
Dong
Shinan
S
Tang
Xudong
X
Xu
Li
L
D000818:Animals; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D009036:Moths / Q000382:microbiology*; D016818:Nosema / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D004275:DNA, Ribosomal