title: [Modified method for purifying Cryptosporidium oocysts].
abstract: Sheather's sucrose solution diluted with distilled water as an alternative to PBS was used to purify oocysts of three different Cryptosporidium spp. The recovery rate of purified Cryptosporidium andersoni, C. baileyi and C. suis oocysts was 47.5%, 49.6% and 41.7%, respectively. The viability of the oocysts was 97.9%, 96.7%, and 95.1%, respectively. After 1 h incubation in a 37 degrees C water bath, the excystation rate of the oocysts was 87.9%, 80.0%, and 81.7%, respectively.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.forename: Chun-xia
authors.initials: CX
authors.lastname: An
authors.forename: Su-mei
authors.initials: SM
authors.lastname: Zhang
authors.forename: Chang-shen
authors.initials: CS
authors.lastname: Ning
authors.forename: Long-xian
authors.initials: LX
authors.lastname: Zhang
mesh_terms: D003458:Cryptosporidium / Q000302:isolation & purification*; D016785:Cryptosporidium parvum / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D033661:Oocysts / Q000469:parasitology*; D010270:Parasite Egg Count / Q000379:methods*