Molecular characterisation and nucleotide sequence analysis of canine parvovirus strains in vaccines in India.
Canine parvovirus 2 (CPV-2) is one of the most important viruses that causes haemorrhagic gastroenteritis and myocarditis of dogs worldwide. The picture has been complicated further due to the emergence of new mutants of CPV, namely: CPV-2a, CPV-2b and CPV-2c. In this study, the molecular characterisation of strains present in the CPV vaccines available on the Indian market was performed using polymerase chain reaction and DNA sequencing. The VP1/VP2 genes of two vaccine strains and a field strain (Bhopal) were sequenced and the nucleotide and the deduced amino acid sequences were compared. The results indicated that the isolate belonged to CPV type 2b and the strains in the vaccines belonged to type CPV-2. From the study, it is inferred that the CPV strain used in commercially available vaccine preparation differed from the strains present in CPV infection in dogs in India.
Nandi
Sukdeb
S
Centre for Animal Disease Research and Diagnosis (CADRAD), Indian Veterinary Research Institute (IVRI), Izatnagar, Uttar Pradesh 243122, India. snandi1901@yahoo.com
Anbazhagan
Rajendra
R
Kumar
Manoj
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005759:Gastroenteritis / Q000662:veterinary / Q000821:virology; D007194:India; D009205:Myocarditis / Q000662:veterinary / Q000821:virology; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000145:classification / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013045:Species Specificity; D014765:Viral Vaccines
D036022:Capsid Proteins; D004279:DNA, Viral; D014765:Viral Vaccines