Genome response to tissue plasminogen activator in experimental ischemic stroke.
BACKGROUND
Tissue plasminogen activator (tPA) is known to have functions beyond fibrinolysis in acute ischemic stroke, such as blood brain barrier disruption. To further delineate tPA functions in the blood, we examined the gene expression profiles induced by tPA in a rat model of ischemic stroke.


RESULTS
tPA differentially expressed 929 genes in the blood of rats (p <or= 0.05, fold change >or= |1.2|). Genes identified had functions related to modulation of immune cells. tPA gene expression was found to be dependent on the reperfusion status of cerebral vasculature. The majority of genes regulated by tPA were different from genes regulated by ischemic stroke.


CONCLUSIONS
tPA modulates gene expression in the blood of rats involving immune cells in a manner that is dependent on the status of vascular reperfusion. These non-fibrinolytic activities of tPA in the blood serve to better understand tPA-related complications.
Jickling
Glen C
GC
Department of Neurology and M,I,N,D, Institute, University of California at Davis, Sacramento, California 95817, USA. gcjickling@ucdavis.edu
Zhan
Xinhua
X
Ander
Bradley P
BP
Turner
Renée J
RJ
Stamova
Boryana
B
Xu
Huichun
H
Tian
Yingfang
Y
Liu
Dazhi
D
Davis
Ryan R
RR
Lapchak
Paul A
PA
Sharp
Frank R
FR
D000818:Animals; D001812:Blood-Brain Barrier; D002545:Brain Ischemia / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics*; D020869:Gene Expression Profiling; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D020521:Stroke / Q000188:drug therapy* / Q000235:genetics*; D010959:Tissue Plasminogen Activator / Q000627:therapeutic use*
D010959:Tissue Plasminogen Activator
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