Design and in vitro characterization of buccoadhesive drug delivery system of insulin.
A buccoadhesive drug delivery system of Insulin was prepared by solvent casting technique and characterized in vitro by surface pH, bioadhesive strength, drug release and skin permeation studies. Sodium carboxymethylcellulose-DVP was chosen as the controlled release matrix polymer. The optimized formulation J(4) contained Sodium carboxy methyl cellulose-DVP 2% (w/v), insulin (50 IU/film), propylene glycol (0.25 ml) and Isopropyl alcohol: water (1:4) as solvent system. Bioadhesive strength of the prepared patches was measured on a modified physical balance using bovine cheek pouch as the model membrane. In vitro release studies were carried out at 37 +/- 2 degrees using phosphate buffer pH 6.6, in a modified dissolution apparatus fabricated for the purpose. Cumulative amount of drug released from the optimized formulation J(4) was 91.64% in 6 hours. In vitro permeation studies were carried out on J(4) at 37 +/- 2 degrees using Franz diffusion cell. Cumulative amount of drug permeated from J(4) was 6.63% in 6 hours. In order to enhance the permeation of protein drug, different permeation enhancers were evaluated. The results suggested that sodium deoxycholate 5% (w/v) was the best permeation enhancer among those evaluated. It enhanced the permeation of insulin from 6.63% to 10.38% over a period of 6 hours. The optimized patches were also satisfactory in terms of surface pH and bioadhesive strength. It can also be easily concluded that the system is a success as compared to the conventional formulations with respect to invasiveness, requirement of trained persons for administration and most importantly, the first pass metabolism.
Sahni
J
J
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard University, New Delhi - 110062, India.
Raj
S
S
Ahmad
F J
FJ
Khar
R K
RK
J Pharm Sci. 1988 Apr;77(4):337-9
3379594
Pharm Res. 1996 May;13(5):779-83
8860437
Biol Pharm Bull. 1996 Aug;19(8):1059-63
8874816
J Drug Target. 1996;4(2):69-78
8894966
Int J Pharm. 2002 May 15;238(1-2):93-103
11996813
Pharm Res. 1996 Aug;13(8):1233-7
8865318
J Pharm Sci. 1997 Oct;86(10):1115-9
9344167
Pharm Dev Technol. 1997 Aug;2(3):265-74
9552454