Screening of epigenetic and genetic disturbances of human chromosome 3 genes in colorectal cancer.
DNA microarray technology comprising NotI-linking clones was used in a large-scale study of genetic and epigenetic changes in colorectal cancer. Analysis of samples from 24 patients revealed methylation, deletions, and amplifications in 137 of 181 NotI clones. For 27 genes/loci, these changes occurred in more than 30% of the tumor samples, suggesting that these genes are involved in the development of colorectal cancer. An analysis of the methylation status of CpG island of the ITGA9 gene/loci by bisulfite sequencing confirmed the NotI microarray data on the gene/loci methylation in colorectal cancer. Aberrations in 19 genes/loci were unknown previously. Their characterization may help ascertain the mechanisms responsible for colorectal cancer development and identify novel diagnostic and prognostic markers.
Gerashchenko
G V
GV
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv. g.v.gerashchenko@imbg.org.ua
Gordiyuk
V V
VV
Skrypkina
I Ya
IY
Kvasha
S M
SM
Kolesnik
O O
OO
Ugryn
D D
DD
Pavlova
T V
TV
Zabarovsky
E R
ER
Rynditch
A V
AV
Kashuba
V I
VI
D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D056426:Genetic Loci / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*