MicroRNAs are expressed and processed by human primary macrophages.
Macrophages are crucial to host defense, functioning in innate and cell-mediated immunity. MicroRNAs (miRNAs) are small non-coding RNA molecules that repress transcription and protein production. Little is known about miRNA expression in primary human macrophages, or about how macrophage miRNAs contribute to both normal macrophage function and to the pathogenesis of disease in humans. Using Western blot analyses, we demonstrated the production of miRNA machinery proteins by human primary macrophages. Using two different miRNA array techniques, we identified 119 miRNAs expressed by human primary macrophages, including hsa-let-7a, miR-16, -23a, 30b, -103, -146a, -212, and -378 and validated them by quantitative RT-PCR. Our findings provide a knowledge base to which macrophage miRNA expression in organ-specific macrophages or disease processes may be compared in humans.
Luers
Aimée J
AJ
Department of Pathology, The Albert Einstein College of Medicine (Einstein), Bronx, NY 10461, USA.
Loudig
Olivier D
OD
Berman
Joan W
JW
D015703:Antigens, CD / Q000378:metabolism; D060565:Argonaute Proteins; D018929:Cell Culture Techniques; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D046228:Microarray Analysis; D009928:Organ Specificity; D011506:Proteins / Q000378:metabolism; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043244:Ribonuclease III / Q000378:metabolism
C466422:AGO2 protein, human; D015703:Antigens, CD; D060565:Argonaute Proteins; C474043:DGCR8 protein, human; D015852:Eukaryotic Initiation Factor-2; D035683:MicroRNAs; D011506:Proteins; D016601:RNA-Binding Proteins; D043244:Ribonuclease III
Blood. 2009 Jan 15;113(3):671-4
19015395
Cell Cycle. 2008 Aug 15;7(16):2485-92
18719380
Mol Cell. 2009 Jun 26;34(6):696-709
19560422
Biotechnol Lett. 2008 Dec;30(12):2045-52
18648749
Am J Pathol. 2009 Mar;174(3):736-45
19179615
Nat Cell Biol. 2007 Jul;9(7):775-87
17589498
PLoS One. 2009 Jun 12;4(6):e5889
19521514
Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1604-9
17242365
Blood. 2006 Nov 15;108(10):3305-12
16857988
BMC Mol Biol. 2007 Jul 30;8:63
17663774
Cardiovasc Res. 2008 Sep 1;79(4):581-8
18550634
Exp Biol Med (Maywood). 2008 Nov;233(11):1454-61
18791127
Hepatology. 2009 Apr;49(4):1098-112
19173277
Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3351-6
18308930
Genes Dev. 2004 Dec 15;18(24):3016-27
15574589
Am J Pathol. 2009 Sep;175(3):1148-59
19661443
Retrovirology. 2005 Aug 18;2:53
16109172
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D140-4
16381832
Cell. 2004 Jan 23;116(2):281-97
14744438
Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7113-8
19359473
Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9740-4
15210942
Nat Cell Biol. 2009 Mar;11(3):228-34
19255566
Methods Mol Biol. 2004;265:131-58
15103073
Carcinogenesis. 2008 Nov;29(11):2073-7
18700235
Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12481-6
16885212