title: Essential loci in centromeric heterochromatin of Drosophila melanogaster. I: the right arm of chromosome 2.
abstract: With the most recent releases of the Drosophila melanogaster genome sequences, much of the previously absent heterochromatic sequences have now been annotated. We undertook an extensive genetic analysis of existing lethal mutations, as well as molecular mapping and sequence analysis (using a candidate gene approach) to identify as many essential genes as possible in the centromeric heterochromatin on the right arm of the second chromosome (2Rh) of D. melanogaster. We also utilized available RNA interference lines to knock down the expression of genes in 2Rh as another approach to identifying essential genes. In total, we verified the existence of eight novel essential loci in 2Rh: CG17665, CG17683, CG17684, CG17883, CG40127, CG41265, CG42595, and Atf6. Two of these essential loci, CG41265 and CG42595, are synonymous with the previously characterized loci l(2)41Ab and unextended, respectively. The genetic and molecular analysis of the previously reported locus, l(2)41Ae, revealed that this is not a single locus, but rather it is a large region of 2Rh that extends from unextended (CG42595) to CG17665 and includes four of the novel loci uncovered here.
authors.affiliation: Department of Biology, York University, Toronto, Ontario M3J 1P3, Canada.
authors.forename: Alistair B
authors.initials: AB
authors.lastname: Coulthard
authors.forename: Christina
authors.initials: C
authors.lastname: Alm
authors.forename: Iulia
authors.initials: I
authors.lastname: Cealiac
authors.forename: Don A
authors.initials: DA
authors.lastname: Sinclair
authors.forename: Barry M
authors.initials: BM
authors.lastname: Honda
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Rossi
authors.forename: Patrizio
authors.initials: P
authors.lastname: Dimitri
authors.forename: Arthur J
authors.initials: AJ
authors.lastname: Hilliker
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D022004:Chromosome Structures; D002875:Chromosomes / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005796:Genes; D006570:Heterochromatin / Q000235:genetics*; D009154:Mutation
chemical_list: D006570:Heterochromatin
references.citation: Science. 1966 Jan 28;151(3709):417-25
references.pmid: 5322971
references.citation: Genetics. 1993 May;134(1):277-92
references.pmid: 8514136
references.citation: Genetics. 2007 Feb;175(2):595-607
references.pmid: 17110485
references.citation: Genetics. 1995 Jul;140(3):1033-45
references.pmid: 7672575
references.citation: Science. 2005 Jan 7;307(5706):108-10
references.pmid: 15528405
references.citation: Genetics. 2004 Jun;167(2):761-81
references.pmid: 15238527
references.citation: Genetics. 2006 Jul;173(3):1433-45
references.pmid: 16648646
references.citation: Genetics. 1990 May;125(1):129-40
references.pmid: 2111263
references.citation: Genetics. 1984 Jul;107(3):423-34
references.pmid: 6428976
references.citation: Nature. 2007 Jul 12;448(7150):151-6
references.pmid: 17625558
references.citation: Mol Biol Evol. 2002 Jun;19(6):926-37
references.pmid: 12032249
references.citation: Science. 2007 Jun 15;316(5831):1586-91
references.pmid: 17569856
references.citation: EMBO J. 1994 Apr 1;13(7):1628-35
references.pmid: 8157002
references.citation: Annu Rev Genet. 1992;26:239-75
references.pmid: 1482113
references.citation: Proc Natl Acad Sci U S A. 1936 Jan;22(1):27-33
references.pmid: 16588038
references.citation: Adv Genet. 1951;4:87-125
references.pmid: 14943674
references.citation: Chromosoma. 2009 Aug;118(4):419-35
references.pmid: 19412619
references.citation: Methods Mol Biol. 2004;260:29-39
references.pmid: 15020800
references.citation: Genetics. 1958 May;43(3):395-403
references.pmid: 17247765
references.citation: Science. 2007 Jun 15;316(5831):1625-8
references.pmid: 17569867
references.citation: Genetics. 2005 Apr;169(4):2165-77
references.pmid: 15687284
references.citation: Dev Cell. 2002 Dec;3(6):865-76
references.pmid: 12479811
references.citation: Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3804-8
references.pmid: 7731987
references.citation: Genetics. 1985 Aug;110(4):671-88
references.pmid: 17246295
references.citation: Genes Dev. 2002 Aug 15;16(16):2108-19
references.pmid: 12183365
references.citation: Genome Biol. 2002;3(12):RESEARCH0085
references.pmid: 12537574
references.citation: Nucleic Acids Res. 2009 Jan;37(Database issue):D555-9
references.pmid: 18948289
references.citation: Chromosoma. 2003 Jul;112(1):26-37
references.pmid: 12827380
references.citation: Genetics. 1999 Jun;152(2):577-93
references.pmid: 10353901
references.citation: Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):10958-63
references.pmid: 16033869
references.citation: Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3481-5
references.pmid: 8475096
references.citation: Genetics. 1991 Aug;128(4):785-97
references.pmid: 1916244
references.citation: Annu Rev Genet. 1995;29:577-605
references.pmid: 8825487
references.citation: Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12405-10
references.pmid: 18713858
references.citation: Trends Genet. 2006 Jun;22(6):330-8
references.pmid: 16690158
references.citation: Genetics. 1991 Mar;127(3):553-64
references.pmid: 1707844
references.citation: Genetics. 2007 Sep;177(1):359-74
references.pmid: 17890366
references.citation: Genetics. 1993 Aug;134(4):1149-74
references.pmid: 8375654
references.citation: Mol Biol Evol. 2003 Apr;20(4):503-12
references.pmid: 12654931
references.citation: Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3539-43
references.pmid: 8170943
references.citation: Genetics. 2005 Feb;169(2):683-95
references.pmid: 15520262
references.citation: Genetics. 2000 Jun;155(2):699-708
references.pmid: 10835392
references.citation: Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8052-7
references.pmid: 9223313
references.citation: Genetics. 1976 Aug;83(4):765-82
references.pmid: 823073
references.citation: Genome. 2003 Jun;46(3):343-52
references.pmid: 12834049
references.citation: Genetics. 2004 Feb;166(2):807-22
references.pmid: 15020470
references.citation: Science. 2000 Mar 24;287(5461):2185-95
references.pmid: 10731132
references.citation: Annu Rev Entomol. 2007;52:171-92
references.pmid: 16881818
references.citation: Cell. 1980 Oct;21(3):607-19
references.pmid: 6777044
references.citation: Chromosoma. 2008 Feb;117(1):51-66
references.pmid: 17909832
references.citation: Genome. 1998 Apr;41(2):236-43
references.pmid: 9644832
references.citation: Genetics. 1975 Dec;81(4):705-21
references.pmid: 814038
references.citation: Genetics. 1988 Sep;120(1):181-98
references.pmid: 3146523
references.citation: Genetica. 2003 Mar;117(2-3):209-15
references.pmid: 12723700
references.citation: Genet Res. 1974 Aug;24(1):1-10
references.pmid: 4373306