title: Brain microvascular endothelial cells mediate neuroprotective effects on ischemia/reperfusion neurons.
abstract: OBJECTIVE
  The permeability of the blood-brain barrier (BBB) is a bottleneck for the development of new cerebropathy medications because the medication must be transmitted across the BBB to achieve its curative function. To explore a new approach to the treatment of brain disease, this study investigated the mediating effects of brain microvascular endothelial cells (MVECs) on injured neurons.
  
  
  METHODS
  MVECs and cortical neurons were cultured and damage by cerebral ischemia/reperfusion (I/R) was simulated. The conditioned media from four groups of MVECs - normal cells (N-CM), normal cells treated with Tong Luo Jiu Nao (TLJN) (NT-CM), simulated cerebral I/R cells (I/R-CM), and simulated cells treated with TLJN (I/RTCM) - were then collected. These conditioned media were added to neuronal cultures and the viability of the neurons was examined.
  
  
  RESULTS
  The results demonstrated that N-CM could alleviate I/R damage to neurons, and this capacity could be improved by TLJN treatment. However, I/R-CM could cause damage to normal and I/R neurons, while I/RT-CM could significantly alleviate the damage to I/R neurons.
  
  
  CONCLUSIONS
  We propose that MVECs secrete active substances that influence the survival of neurons, and so MVECs may mediate a neuroprotective effect on ischemia/reperfusion neurons.
authors.affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Hua
authors.forename: Xuemei
authors.initials: X
authors.lastname: Qing
authors.forename: Pengtao
authors.initials: P
authors.lastname: Li
authors.forename: Weihong
authors.initials: W
authors.lastname: Li
authors.forename: Jincai
authors.initials: J
authors.lastname: Hou
authors.forename: Jinghong
authors.initials: J
authors.lastname: Hu
authors.forename: Qingtao
authors.initials: Q
authors.lastname: Hong
authors.forename: Ping
authors.initials: P
authors.lastname: Sun
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology; D002545:Brain Ischemia / Q000473:pathology / Q000517:prevention & control; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D055806:Microvessels / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D008954:Models, Biological; D015394:Molecular Structure; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000473:pathology*; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control
chemical_list: D017077:Culture Media, Conditioned; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C528243:tongluo jiunao