title: Effect of trace iron levels and iron withdrawal by chelation on the growth of Candida albicans and Candida vini.
abstract: The iron requirements of the opportunistic pathogenic yeast, Candida albicans, and the related nonpathogenic spoilage yeast Candida vini were investigated along with their responses to various exogenous iron chelators. The influence of iron as well as the exogenous chelating agents lactoferrin, EDTA, deferiprone, desferrioxamine, bathophenanthroline sulphonate and a novel carried chelator with a hydroxypyridinone-like Fe-ligand functionality, DIBI, on fungal growth was studied in a chemically defined medium deferrated to trace iron levels (<1.2 microg L(-1) or 0.02 microM of Fe). Candida albicans competed better at low iron levels compared with C. vini, which was also more susceptible to most added chelators. Candida albicans was resistant to lactoferrin at physiologically relevant concentrations, but was inhibited by low concentrations of DIBI. Candida vini was sensitive to lactoferrin as well as to DIBI, whose inhibitory activity was shown to be Fe reversible. The pathogenic potential of C. albicans and the nonpathogenic nature of C. vini were consistent with their differing abilities to grow under iron-limiting conditions and in the presence of exogenous iron chelators. Both yeasts could be controlled by appropriately strong chelators. This work provides the first evidence of the iron requirements of the spoilage organism C. vini and its response to exogenous chelators. Efficient iron withdrawal has the potential to provide the basis for new fungal growth control strategies.
authors.affiliation: Chelation Partners, Guelph, ON, Canada.
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Holbein
authors.forename: Ramón
authors.initials: R
authors.lastname: Mira de Orduña
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D002175:Candida / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D006131:Growth Inhibitors / Q000378:metabolism / Q000494:pharmacology; D007501:Iron / Q000032:analysis* / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D006131:Growth Inhibitors; D007502:Iron Chelating Agents; D007501:Iron