title: Early effects of low dos C ion or X-ray irradiation on human peripheral blood lymphocytes.
abstract: The aim of this study was to estimate the acute effects of low dose (12)C(6+) ions or X-ray radiation on human immune function. The human peripheral blood lymphocytes (HPBL) of seven healthy donors were exposed to 0.05Gy (12)C(6+) ions or X-ray radiation and cell responses were measured at 24 hours after exposure. The cytotoxic activities of HPBL were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT); the percentages of T and NK cells subsets were detected by flow cytometry; mRNA expression of interleukin (IL)-2, tumor necrosis factor (TNF)-α and interferon (IFN)-γ were examined by real time quantitative RT-PCR (qRT-PCR); and these cytokines protein levels in supernatant of cultured cells were assayed by enzyme-linked immunosorbent assays (ELISA). The results showed that the cytotoxic activity of HPBL, mRNA expression of IL-2, IFN-γ and TNF-α in HPBL and their protein levels in supernatant were significantly increased at 24 hours after exposure to 0.05Gy (12)C(6+) ions radiation and the effects were stronger than observed for X-ray exposure. However, there was no significant change in the percentage of T and NK cells subsets of HPBL. These results suggested that 0.05Gy high linear energy transfer (LET) (12)C(6+) radiation was a more effective approach to host immune enhancement than that of low LET X-ray. We conclude that cytokines production might be used as sensitive indicators of acute response to LDI.
authors.affiliation: College of Clinical Medicine of Lanzhou University. 222 Tianshui Nan Road, Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Yingtai
authors.initials: Y
authors.lastname: Chen
authors.forename: Yumin
authors.initials: Y
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Xuezhong
authors.initials: X
authors.lastname: Chen
authors.forename: Jinyu
authors.initials: J
authors.lastname: Ren
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.forename: Zijiang
authors.initials: Z
authors.lastname: Zhu
authors.forename: Hongliang
authors.initials: H
authors.lastname: Liu
authors.forename: Yawei
authors.initials: Y
authors.lastname: Zhang
references.citation: Biomed Environ Sci. 1994 Jun;7(2):130-5
references.pmid: 7946008
references.citation: J Med. 1994;25(1-2):23-40
references.pmid: 7930958
references.citation: Radiat Res. 1999 Jun;151(6):717-24
references.pmid: 10360792
references.citation: Pharmacol Ther. 1990;45(1):137-51
references.pmid: 2105509
references.citation: J Gerontol A Biol Sci Med Sci. 2004 Jul;59(7):705-9
references.pmid: 15304535
references.citation: Adv Space Res. 2004;34(6):1368-72
references.pmid: 15881649
references.citation: Int J Radiat Biol. 2007 Sep;83(9):577-81
references.pmid: 17654099
references.citation: Hum Exp Toxicol. 2000 Jan;19(1):76-84
references.pmid: 10745295
references.citation: Immunol Rev. 2000 Apr;174:135-49
references.pmid: 10807513
references.citation: Science. 1988 May 27;240(4856):1169-76
references.pmid: 3131876
references.citation: FEBS Lett. 2005 May 9;579(12):2731-6
references.pmid: 15862317
references.citation: Science. 1984 Feb 10;223(4636):594-7
references.pmid: 6695170
references.citation: Nihon Igaku Hoshasen Gakkai Zasshi. 1997 Jun;57(7):418-24
references.pmid: 9232991
references.citation: Immunity. 2005 Mar;22(3):343-54
references.pmid: 15780991
references.citation: Health Phys. 1990 Jul;59(1):11-3
references.pmid: 2358350
references.citation: Lymphokine Cytokine Res. 1991 Apr;10(1-2):105-9
references.pmid: 1908321
references.citation: Int J Radiat Biol. 1993 Jun;63(6):775-83
references.pmid: 8100265
references.citation: Clin Exp Immunol. 1998 Dec;114(3):434-47
references.pmid: 9844055
references.citation: Science. 2006 Sep 29;313(5795):1960-4
references.pmid: 17008531
references.citation: Blood. 1996 Mar 15;87(6):2095-147
references.pmid: 8630372
references.citation: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references.pmid: 6606682
references.citation: Immunol Lett. 2002 Jan 1;80(1):55-66
references.pmid: 11716966
references.citation: Mutat Res. 1991 Jan;246(1):179-86
references.pmid: 1986262
references.citation: Crit Rev Toxicol. 1999 Sep;29(5):491-511
references.pmid: 10521134
references.citation: Cancer Immunol Immunother. 2009 May;58(5):801-8
references.pmid: 19089424
references.citation: J Immunol. 1982 Jan;128(1):116-22
references.pmid: 6172473
references.citation: Hum Exp Toxicol. 2003 Jun;22(6):290-306; discussion 307, 315-7, 319-23
references.pmid: 12856953
references.citation: Microbes Infect. 2005 Feb;7(2):224-32
references.pmid: 15777634
references.citation: Cell Immunol. 2006 Nov;244(1):50-6
references.pmid: 17420010
references.citation: Radiat Prot Dosimetry. 2002;99(1-4):413-8
references.pmid: 12194343
references.citation: Mutagenesis. 1987 Mar;2(2):95-6
references.pmid: 3137419
references.citation: Cancer. 1996 Apr 1;77(7):1303-10
references.pmid: 8608507