title: Requirement for BAFF and APRIL during B cell development in GALT.
abstract: The effects of B cell-activating factor belonging to the TNF family (BAFF) on B cell maturation and survival in the mouse are relatively well understood. In contrast, little is known about the role of BAFF in B cell development in other mammals, such as rabbits, that use GALT to develop and maintain the B cell compartment. We examined the expression and requirement of BAFF and a proliferation-inducing ligand (APRIL) during peripheral B cell development in young rabbits. By neutralizing BAFF and APRIL in neonates with a soluble decoy receptor, transmembrane activator calcium modulator and cyclophilin ligand interactor-Fc, we found a marked reduction in the number of peripheral B cells, but found no change in the bone marrow (BM) compartment. In the appendix, the size and number of proliferating B cell follicles were greatly reduced, demonstrating that although BAFF/APRIL is dispensable for B cell development in BM, it is required for B cell development in GALT. We found that all rabbit B cells expressed BAFF receptor 3, but did not bind rBAFF, suggesting that the BAFF-binding receptors (BBRs) are bound by endogenous soluble BAFF. Further, we found that B cells themselves express BAFF, suggesting that the soluble BAFF bound to BBRs may be endogenously produced and stimulate B cells in an autocrine fashion. Additionally, we propose that this chronic occupancy of BBRs on B cells may provide a tonic and/or survival signal for the maintenance of peripheral B cells in adults after B lymphopoiesis is arrested in BM.
authors.affiliation: Department of Microbiology and Immunology, Strich School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA.
authors.forename: Venkata A
authors.initials: VA
authors.lastname: Yeramilli
authors.forename: Katherine L
authors.initials: KL
authors.lastname: Knight
mesh_terms: D000375:Aging / Q000276:immunology; D000818:Animals; D000831:Animals, Newborn; D053264:B-Cell Activating Factor / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology*; D053265:B-Cell Activation Factor Receptor / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D016466:CHO Cells; D002454:Cell Differentiation / Q000276:immunology*; D002460:Cell Line; D049109:Cell Proliferation; D006224:Cricetinae; D003412:Cricetulus; D041981:Gastrointestinal Tract / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D038041:Lymphopoiesis / Q000276:immunology; D011817:Rabbits; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13 / Q000378:metabolism / Q000502:physiology*
chemical_list: D053264:B-Cell Activating Factor; D053265:B-Cell Activation Factor Receptor; D008024:Ligands; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13
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