Hybrid PET-optical imaging using targeted probes.
Fusion imaging of radionuclide-based molecular (PET) and structural data [x-ray computed tomography (CT)] has been firmly established. Here we show that optical measurements [fluorescence-mediated tomography (FMT)] show exquisite congruence to radionuclide measurements and that information can be seamlessly integrated and visualized. Using biocompatible nanoparticles as a generic platform (containing a (18)F isotope and a far red fluorochrome), we show good correlations between FMT and PET in probe concentration (r(2) > 0.99) and spatial signal distribution (r(2) > 0.85). Using a mouse model of cancer and different imaging probes to measure tumoral proteases, macrophage content and integrin expression simultaneously, we demonstrate the distinct tumoral locations of probes in multiple channels in vivo. The findings also suggest that FMT can serve as a surrogate modality for the screening and development of radionuclide-based imaging agents.
Nahrendorf
Matthias
M
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. MNahrendorf@mgh.harvard.edu
Keliher
Edmund
E
Marinelli
Brett
B
Waterman
Peter
P
Feruglio
Paolo Fumene
PF
Fexon
Lioubov
L
Pivovarov
Misha
M
Swirski
Filip K
FK
Pittet
Mikael J
MJ
Vinegoni
Claudio
C
Weissleder
Ralph
R
D000818:Animals; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles; D009369:Neoplasms / Q000175:diagnosis*; D049268:Positron-Emission Tomography / Q000379:methods*; D014057:Tomography, X-Ray Computed / Q000379:methods*
D005456:Fluorescent Dyes
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