title: A vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor.
abstract: Inducible resistance to the glycopeptide antibiotic vancomycin requires expression of vanH, vanA and vanX, controlled by a two-component regulatory system consisting of a receptor histidine kinase, VanS, and a response regulator, VanR. The identity of the VanS receptor ligand has been debated. Using a synthesized vancomycin photoaffinity probe, we show that vancomycin directly binds Streptomyces coelicolor VanS (VanSsc) and this binding is correlated with resistance and required for vanH, vanA and vanX gene expression.
authors.affiliation: M.G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Kalinka
authors.initials: K
authors.lastname: Koteva
authors.forename: Hee-Jeon
authors.initials: HJ
authors.lastname: Hong
authors.forename: Xiao Dong
authors.initials: XD
authors.lastname: Wang
authors.forename: Ishac
authors.initials: I
authors.lastname: Nazi
authors.forename: Donald
authors.initials: D
authors.lastname: Hughes
authors.forename: Mike J
authors.initials: MJ
authors.lastname: Naldrett
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Buttner
authors.forename: Gerard D
authors.initials: GD
authors.lastname: Wright
mesh_terms: D000071677:Histidine Kinase; D015335:Molecular Probes; D019920:Photoaffinity Labels; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D011955:Receptors, Drug / Q000378:metabolism*; D014640:Vancomycin / Q000378:metabolism*
chemical_list: D015335:Molecular Probes; D019920:Photoaffinity Labels; D011955:Receptors, Drug; D014640:Vancomycin; D011494:Protein Kinases; D000071677:Histidine Kinase
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