title: Impact of Pseudomonas aeruginosa genomic instability on the application of typing methods for chronic cystic fibrosis infections.
abstract: The Liverpool epidemic strain (LES) of Pseudomonas aeruginosa is widespread among cystic fibrosis (CF) patients in the United Kingdom and has emerged recently in North America. In this study, we report the analysis of 24 "anomalous" CF isolates of P. aeruginosa that produced inconsistent results with regard to either pulsed-field gel electrophoresis (PFGE) or PCR tests for the LES. We used a new typing method, the ArrayTube genotyping system, to determine that of the 24 anomalous isolates tested, 13 were confirmed as the LES. LES isolates could not be clearly distinguished from non-LES isolates by two other commonly used genetic fingerprinting tests, randomly amplified polymorphic DNA (RAPD) analysis and BOX-PCR, and varied considerably in their carriage of LES genomic islands and prophages. The genomic instability of the LES suggests that identification of this emerging transmissible strain could be a challenging task, and it questions whether discrimination is always a desirable feature of bacterial typing methods in the context of chronic CF infections.
authors.affiliation: Division of Medical Microbiology, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, United Kingdom.
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Fothergill
authors.forename: Judith
authors.initials: J
authors.lastname: White
authors.forename: Juliet E
authors.initials: JE
authors.lastname: Foweraker
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Walshaw
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Ledson
authors.forename: Eshwar
authors.initials: E
authors.lastname: Mahenthiralingam
authors.forename: Craig
authors.initials: C
authors.lastname: Winstanley
mesh_terms: D015373:Bacterial Typing Techniques; D003550:Cystic Fibrosis / Q000150:complications*; D016172:DNA Fingerprinting / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field; D042822:Genomic Instability; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D009656:North America; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique; D006113:United Kingdom
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