Detection of hetero-proteins-mesoporous silica assembly by BRET.
The assembly of a hetero-protein (Renilla reniformis luciferase (Rluc) and a green fluorescence protein (sGFP)) encapsulated in folded-sheet mesoporous material with 7.1 nm pore diameter (FSM7.1), which was used for studying protein-protein interactions in pores of mesoporous silica, has been confirmed by the detection of bioluminescence resonance energy transfers (BRET).
Matsuura
Shun-ichi
S
Research Center for Compact Chemical Process, National Institute of Advanced Industrial Science and Technology (AIST), Nigatake 4-2-1, Miyagino-ku, Sendai, 983-8551, Japan. matsuura-shunichi@aist.go.jp
Tsunoda
Tatsuo
T
Shiomi
Toru
T
Sakaguchi
Kengo
K
Hanaoka
Takaaki
T
Mizukami
Fujio
F
D031541:Fluorescence Resonance Energy Transfer; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008156:Luciferases / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D016062:Porosity; D011485:Protein Binding; D012822:Silicon Dioxide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D049452:Green Fluorescent Proteins; D012822:Silicon Dioxide; D008156:Luciferases