Perinatal cisplatin exposure induces cochlear apoptosis in newborn guinea pigs.
The objective of this study was to evaluate the role of apoptosis in the development of the newborn cochlear structures and hearing loss caused by prenatal cis-diaminedichloroplatinum (cisplatin) exposure. Pregnant albino guinea pigs were intraperitoneally injected with 1.5 mg/kg body weight cisplatin once a day for seven consecutive days at gestational day (GD) 51 to GD 57. At postnatal day (PND) 14, pups were examined in the distortion product otoacoustic emission (DPOAE) task. The temporal bones were then removed and immunohistochemically stained for caspase 3, using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method. Cisplatin used during pregnancy could induce hearing loss in newborn and cochlear hair cell apoptosis. In conclusion, apoptosis may play an important role in the development of hearing impairment, caused by perinatal cisplatin exposure.
Hao
Shuai
S
Department of Otolaryngology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China.
Jiang
Xuejun
X
Yan
Aihui
A
Yang
Bo
B
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002945:Cisplatin / Q000008:administration & dosage / Q000633:toxicity*; D003051:Cochlea / Q000196:embryology / Q000473:pathology*; D005260:Female; D006168:Guinea Pigs; D034381:Hearing Loss / Q000139:chemically induced*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007274:Injections, Intraperitoneal; D008297:Male; D017084:Otoacoustic Emissions, Spontaneous; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects
D053148:Caspase 3; D002945:Cisplatin