Lipopolysaccharide induction of cyclooxygenase-2 in long-term nasal epithelial cultures in cystic fibrosis.
OBJECTIVE
To evaluate the lipopolysaccharide induction of cyclooxygenase-2 (COX-2) in long-term epithelial cultures from nasal polyp tissue of patients with cystic fibrosis (CF) and severe nasal polyposis.


METHODS
Experimental and histologic study.


METHODS
Department of Otorhinolaryngology, University of Ulm, Ulm, Germany.


METHODS
Nasal polyp tissue was evaluated from 9 patients with CF and obstructing nasal polyps undergoing elective sinus surgery. Nasal mucosa from the hypertrophic inferior turbinate of 9 patients without a history of CF or aspirin intolerance undergoing nasal corrective surgery served as control specimens.


METHODS
Tissue culturing, Western blotting, and tissue staining with hematoxylin-eosin.


METHODS
The expression and lipopolysaccharide induction of COX-2 was detected and compared with those of the control group. Tissue was analyzed for the presence of inflammatory cells such as neutrophils, eosinophils, mast cells, plasma cells, lymphocytes, and monocytes.


RESULTS
COX-2 was detectable in tissue specimens from all of the patients with CF and control subjects. In patients with CF, however, COX-2 expression was significantly lower in lipopolysaccharide-stimulated, long-term cultured epithelial cells compared with control tissue. Polyps from patients with CF contained markedly more neutrophils, macrophages, and plasma cells than did nasal mucosa from hypertrophic inferior turbinates of controls.


CONCLUSIONS
COX-2 expression in lipopolysaccharide-stimulated, long-term cultured epithelial cells in patients with CF is decreased compared with that in hypertrophic turbinate mucosa. The estimated defect in lipopolysaccharide responsiveness and the reduced induction of COX-2 needs further investigation.
Rozsasi
Ajnacska
A
Department of Otorhinolaryngology, University of Ulm Medical School, Germany. ajnirozsasi@aol.com
Keck
Tilman
T
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D018929:Cell Culture Techniques; D002648:Child; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000150:complications / Q000201:enzymology* / Q000473:pathology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000201:enzymology; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D009297:Nasal Mucosa / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D009298:Nasal Polyps / Q000201:enzymology* / Q000209:etiology / Q000473:pathology; D013997:Time Factors; D055815:Young Adult
D008070:Lipopolysaccharides; D051546:Cyclooxygenase 2