title: Sequence-specific recognition of methylated DNA by human zinc-finger proteins.
abstract: DNA methylation is an essential epigenetic mark. Three classes of mammalian proteins recognize methylated DNA: MBD proteins, SRA proteins and the zinc-finger proteins Kaiso, ZBTB4 and ZBTB38. The last three proteins can bind either methylated DNA or unmethylated consensus sequences; how this is achieved is largely unclear. Here, we report that the human zinc-finger proteins Kaiso, ZBTB4 and ZBTB38 can bind methylated DNA in a sequence-specific manner, and that they may use a mode of binding common to other zinc-finger proteins. This suggests that many other sequence-specific methyl binding proteins may exist.
authors.affiliation: CNRS UMR7216, Université Paris-Diderot, Paris, France.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Sasai
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Nakao
authors.forename: Pierre-Antoine
authors.initials: PA
authors.lastname: Defossez
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D016384:Consensus Sequence; D019175:DNA Methylation; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C492254:ZBTB33 protein, human; C508975:ZBTB38 protein, human; C508974:ZBTB4 protein, human
references.citation: Mol Cell Biol. 2004 Aug;24(16):7188-96
references.pmid: 15282317
references.citation: Nature. 2009 Nov 19;462(7271):315-22
references.pmid: 19829295
references.citation: Genes Dev. 2001 Jul 1;15(13):1613-8
references.pmid: 11445535
references.citation: Science. 2009 Jun 26;324(5935):1720-3
references.pmid: 19443739
references.citation: Development. 2009 Mar;136(5):729-38
references.pmid: 19158185
references.citation: Structure. 2001 Aug;9(8):717-23
references.pmid: 11587646
references.citation: Nucleic Acids Res. 1986 Feb 25;14(4):1599-614
references.pmid: 3005977
references.citation: Epigenetics. 2009 Jan;4(1):8-14
references.pmid: 19077538
references.citation: Nature. 2007 Dec 6;450(7171):908-12
references.pmid: 17994007
references.citation: BMC Evol Biol. 2008 Jun 18;8:176
references.pmid: 18559114
references.citation: Exp Cell Res. 2005 May 1;305(2):253-65
references.pmid: 15817151
references.citation: Nat Genet. 2009 Feb;41(2):178-186
references.pmid: 19151715
references.citation: Mol Cell. 2008 Feb 29;29(4):525-31
references.pmid: 18313390
references.citation: J Biol Chem. 2010 Jan 8;285(2):1393-403
references.pmid: 19887448
references.citation: Int J Dev Biol. 2009;53(2-3):323-34
references.pmid: 19412889
references.citation: Cell Mol Life Sci. 2008 May;65(10):1509-22
references.pmid: 18322651
references.citation: Mol Cell Biol. 1999 Aug;19(8):5383-92
references.pmid: 10409729
references.citation: Nat Struct Biol. 1998 Apr;5(4):264-5
references.pmid: 9546213
references.citation: Cancer Res. 2008 Sep 15;68(18):7258-63
references.pmid: 18794111
references.citation: Trends Biochem Sci. 2006 Feb;31(2):89-97
references.pmid: 16403636
references.citation: Mol Cell Biol. 1993 Nov;13(11):6810-8
references.pmid: 8413274
references.citation: EMBO J. 2008 Jun 4;27(11):1563-74
references.pmid: 18451802
references.citation: Mol Cell. 2005 Sep 2;19(5):667-78
references.pmid: 16137622
references.citation: Mol Cell. 2003 Sep;12(3):723-34
references.pmid: 14527417
references.citation: PLoS One. 2009;4(1):e4315
references.pmid: 19177165
references.citation: Nature. 2010 Jan 28;463(7280):563-7
references.pmid: 20081831
references.citation: Nucleic Acids Res. 2002 Jul 1;30(13):2911-9
references.pmid: 12087177
references.citation: Mol Cell Biol. 2006 Jan;26(1):169-81
references.pmid: 16354688
references.citation: Genome Res. 2004 Jun;14(6):1188-90
references.pmid: 15173120
references.citation: Cancer Res. 2007 Mar 15;67(6):2577-85
references.pmid: 17363576
references.citation: Mol Cell Biol. 2006 Jan;26(1):199-208
references.pmid: 16354691
references.citation: Science. 2007 Sep 21;317(5845):1760-4
references.pmid: 17673620