Quantum dots decorated with pathogen associated molecular patterns as fluorescent synthetic pathogen models.
We attached the pathogen associated molecular pattern Kdo(2)-Lipid A (the lipopolysaccharide (LPS) from Escherichia coli (E. coli)) to QDs by hydrophobic interactions to synthetically mimic the surface of E. coli. QD-LPS conjugates bind, are taken up and activate effectively macrophages in vitro and they have potent immunostimulatory activity in vivo.
Barr
Tom A
TA
Institute of Immunology & Infection Research, School of Biological Science, University of Edinburgh, Edinburgh, United Kingdom EH9 3JT.
Krembuszewski
Martin
M
Gupta
Manish
M
Gray
David
D
Mareque-Rivas
Juan C
JC
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D004926:Escherichia coli / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008050:Lipid A / Q000737:chemistry* / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D045663:Quantum Dots
D000276:Adjuvants, Immunologic; D008050:Lipid A
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