Hsp60 and Hsp10 increase in colon mucosa of Crohn’s disease and ulcerative colitis.
The purpose of this work was to determine in colon mucosa of Crohn’s disease (CD) and ulcerative colitis (UC) in relapse: a) the levels of the chaperonins Hsp60 and Hsp10; b) the quantity of inflammatory cells; and c) if the levels of chaperonins parallel those of inflammation cells. Twenty cases of CD and UC and twenty normal controls (NC) were studied using immunohistochemistry, Western blotting and immunofluorescence. Immunohistochemically, Hsp60 and Hsp10 were increased in both inflammatory bowel diseases (IBD) compared to NC. These results were confirmed by Western blotting. Hsp60 and Hsp10 occurred in the cytoplasm of epithelial cells in CD and UC but not in NC. Hsp60 and Hsp10 co-localised to epithelial cells of mucosal glands but not always in connective tissue cells of lamina propria, where only Hsp60 or, less often, Hsp10 was found. Cells typical of inflammation were significantly more abundant in CD and UC than in NC. Since chaperonins are key factors in the activation of the immune system leading to inflammation, we propose that they play a central role in the pathogenesis of the two diseases, which, consequently, ought to be studied as chaperonopathies.
Rodolico
Vito
V
Dipartimento di Patologia Umana, Università degli Studi di Palermo, Palermo, Italy.
Tomasello
Giovanni
G
Zerilli
Monica
M
Martorana
Anna
A
Pitruzzella
Alessandro
A
Gammazza
Antonella Marino
AM
David
Sabrina
S
Zummo
Giovanni
G
Damiani
Provvidenza
P
Accomando
Salvatore
S
Conway de Macario
Everly
E
Macario
Alberto J L
AJ
Cappello
Francesco
F
D000328:Adult; D000368:Aged; D018835:Chaperonin 10 / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000378:metabolism*; D003424:Crohn Disease / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged
D018835:Chaperonin 10; D018834:Chaperonin 60
Cancer Lett. 2003 Jun 30;196(1):35-41
12860287
Cell Stress Chaperones. 2006 Winter;11(4):287-94
17278877
Life Sci. 2010 Jan 30;86(5-6):145-52
19913561
PLoS One. 2009 Nov 02;4(11):e7714
19888320
BMC Cancer. 2005 Oct 28;5:139
16253146
Novartis Found Symp. 2008;291:86-95; discussion 96-100, 137-40
18575268
PLoS Pathog. 2009 Aug;5(8):e1000552
19714222
Anticancer Res. 2003 Mar-Apr;23(2B):1325-31
12820390
Immunol Rev. 1991 Jun;121:5-28
1937534
Hum Genet. 2003 Jan;112(1):71-7
12483302
Clin Exp Immunol. 2008 May;152(2):245-51
18336589
N Engl J Med. 2005 Oct 6;353(14):1489-501
16207851
J Gastroenterol. 2006 Jan;41(1):10-6
16501852
EMBO J. 2008 Jan 23;27(2):306-14
18216873
J Histochem Cytochem. 1998 Mar;46(3):397-403
9487122
Int Immunol. 2008 Mar;20(3):433-43
18256040
Biol Chem. 2009 Aug;390(8):723-30
19453276
Med Clin North Am. 2010 Jan;94(1):1-18
19944795
Cell Stress Chaperones. 2010 Mar;15(2):123-41
19731087
Dig Dis Sci. 1998 May;43(5):1079-87
9590425
Dig Dis Sci. 1999 Jul;44(7):1440-7
10489932
Ann N Y Acad Sci. 2007 Oct;1113:178-91
17483209
Inflamm Bowel Dis. 2009 Jan;15(1):100-13
18623167
J Immunol. 2005 Mar 15;174(6):3306-16
15749862
Dig Dis Sci. 1997 Apr;42(4):833-46
9125658
Trends Biochem Sci. 2008 Feb;33(2):71-9
18182297
J Exp Med. 1999 Jan 4;189(1):179-85
9874574
Cell Stress Chaperones. 2005 Winter;10(4):263-4
16333979
Chin J Dig Dis. 2004;5(2):45-50
15612656
Lancet. 2005 Jul 2-8;366(9479):50-6
15993233
Clin Exp Immunol. 1992 Nov;90(2):271-4
1424286
Ann N Y Acad Sci. 2010 Jun;1197:85-93
20536837
Dig Dis Sci. 1994 Mar;39(3):498-506
7907543
J Immunol. 2000 Jan 15;164(2):558-61
10623794
Gut. 1994 May;35(5):669-74
8200563
Biochem Biophys Res Commun. 2006 Sep 15;348(1):1-5
16875664
J Biol Chem. 2005 Feb 11;280(6):4037-47
15546885
Circulation. 2009 Nov 17;120(20):2012-24
19917895
Scand J Gastroenterol. 2000 Nov;35(11):1188-93
11145291
Blood. 2008 Jan 15;111(2):894-904
17962513
Gastroenterology. 1995 Jan;108(1):75-82
7528700
Clin Exp Immunol. 2005 Feb;139(2):371-8
15654837
Fam Med. 2005 May;37(5):360-3
15883903
J Biochem. 2009 Oct;146(4):455-61
19666648
Eur J Histochem. 2003;47(2):105-10
12777205
Inflamm Bowel Dis. 2006 Nov;12(11):1068-83
17075348
J Immunol. 2000 Jan 1;164(1):13-7
10604986
PLoS One. 2010 Feb 16;5(2):e9247
20169074
Gastroenterology. 1991 Sep;101(3):670-8
1860632
J Immunol. 2002 Sep 1;169(5):2422-9
12193710
Cancer Biol Ther. 2008 Jun;7(6):801-9
18497565
Nat Rev Immunol. 2003 Jul;3(7):521-33
12876555
Pathobiology. 2002-2003;70(2):83-8
12476033
J Biol Chem. 2007 Feb 16;282(7):4669-4680
17164250
Ann N Y Acad Sci. 2007 Oct;1113:217-37
17584980