title: Antioxidative and immunoprotective effects of Pyracantha fortuneana (Maxim.) Li polysaccharides in mice.
abstract: This study was undertaken to determine the effects of Pyracantha fortuneana (Maxim.) Li polysaccharides (PFP) on antioxidant and immune functions in mice. Results from this study showed that PFP administration significantly increased thymus and spleen indices, promoted splenocyte proliferation and natural killer (NK) cell activity, and elevated CD4 T cell numbers as well as CD4(+)/CD8(+) ratios. PFP also increased interleukin-2 (IL-2) levels, and decreased the levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in splenocytes. In addition, PFP treatment led to remarkable increases in glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) activities, and dramatic decreases in malondialdehyde (MDA) levels in splenocytes. Moreover, PFP increased mRNA and protein expression of nuclear factor erythroid 2-related factor (Nrf2) in splenocytes. Taken together, these results suggest that PFP treatment enhances the immune function and decreases the oxidative stress in mice.
authors.affiliation: Molecular Oncology and Epigenetics Laboratory, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, People's Republic of China. yuancf46@163.com
authors.forename: Chengfu
authors.initials: C
authors.lastname: Yuan
authors.forename: Changdong
authors.initials: C
authors.lastname: Wang
authors.forename: Youquan
authors.initials: Y
authors.lastname: Bu
authors.forename: Tingxiu
authors.initials: T
authors.lastname: Xiang
authors.forename: Xiuning
authors.initials: X
authors.lastname: Huang
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.forename: Faping
authors.initials: F
authors.lastname: Yi
authors.forename: Guosheng
authors.initials: G
authors.lastname: Ren
authors.forename: Geli
authors.initials: G
authors.lastname: Liu
authors.forename: Fangzhou
authors.initials: F
authors.lastname: Song
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005638:Fruit; D005979:Glutathione Peroxidase / Q000378:metabolism; D007109:Immunity / Q000187:drug effects; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011134:Polysaccharides / Q000008:administration & dosage*; D031991:Pyracantha; D013154:Spleen / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology
chemical_list: D016207:Cytokines; D051267:NF-E2-Related Factor 2; D011134:Polysaccharides; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase