Cellulases and xylanases production by Penicillium echinulatum grown on sugar cane bagasse in solid-state fermentation.
To investigate the production of cellulases and xylanases from Penicillium echinulatum 9A02S1, solid-state fermentation (SSF) was performed by using different ratios of sugar cane bagasse (SCB) and wheat bran (WB). The greatest filter paper activity obtained was 45.82 ± 1.88 U gdm(-1) in a culture containing 6SCB/4WB on the third day. The greatest β-glucosidase activities were 40.13 ± 5.10 U gdm(-1) obtained on the third day for the 0SCB/10WB culture and 29.17 ± 1.06 U gdm(-1) for the 2SCB/8WB culture. For endoglucanase, the greatest activities were 290.47 ± 43.57 and 276.84 ± 15.47 U gdm(-1), for the culture 6SCB/4WB on the fourth and fifth days of cultivation, respectively. The greatest xylanase activities were found on the third day for the cultures 6SCB/4WB (36.38 ± 5.38 U gdm(-1)) and 4SCB/6WB (37.87 ± 2.26 U gdm(-1)). In conclusion, the results presented in this article showed that it was possible to obtain large amounts of cellulases and xylanases enzymes using low-cost substrates, such as SCB and WB.
Camassola
Marli
M
Institute of Biotechnology, University of Caxias do Sul, Brazil. mcamasso@ucs.br
Dillon
Aldo J P
AJ
D044602:Cellulases / Q000737:chemistry / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D007220:Industrial Waste / Q000032:analysis; D007700:Kinetics; D010407:Penicillium / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*
D005656:Fungal Proteins; D007220:Industrial Waste; D044602:Cellulases; D043364:Endo-1,4-beta Xylanases