Applications of atomic force microscopy in biophysical chemistry of cells.
This article addresses the question of what information and new insights atomic force microscopy (AFM) provides that are of importance and relevance to cellular biophysical chemistry research. Three enabling aspects of AFM are discussed: (a) visualization of membrane structural features with nanometer resolution, such as microvilli, ridges, porosomes, lamellapodia, and filopodia; (b) revealing structural evolution associated with cellular signaling pathways by time-dependent and high-resolution imaging of the cellular membrane in correlation with intracellular components from simultaneous optical microscopy; and (c) qualitative and quantitative measurements of single cell mechanics by acquisition of force-deformation profiles and extraction of Young's moduli for the membrane as well as cytoskeleton. A future prospective of AFM is also presented.
Deng
Zhao
Z
Department of Chemistry, University of California, Davis, Davis, California 95616, USA.
Lulevich
Valentin
V
Liu
Fu-tong
FT
Liu
Gang-yu
GY
D000818:Animals; D001703:Biophysics; D002462:Cell Membrane / Q000648:ultrastructure*; D006801:Humans; D008407:Mast Cells / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force
Biophys J. 1996 Oct;71(4):2155-7
8889191
Cell. 2003 Nov 14;115(4):377-88
14622593
Mol Cells. 2008 Dec 31;26(6):517-29
19011361
Biophys J. 1994 Aug;67(2):929-36
7948707
Phys Rev E Stat Nonlin Soft Matter Phys. 2004 Mar;69(3 Pt 1):031915
15089330
Nat Rev Microbiol. 2008 Sep;6(9):674-80
18622407
Ultramicroscopy. 2006 Jun-Jul;106(8-9):645-51
16677764
Biophys J. 2009 Feb 18;96(4):1629-39
19217878
J Chem Phys. 2004 Feb 22;120(8):3822-6
15268547
Agents Actions. 1984 Dec;15(5-6):482-7
6085217
Biochem J. 1992 Mar 1;282 ( Pt 2):545-50
1372167
J Biomech. 2002 Feb;35(2):177-87
11784536
J Mol Biol. 1995 Jun 2;249(2):239-43
7783190
Nat Methods. 2005 Jul;2(7):515-20
15973422
J Cell Sci. 2000 Nov;113 Pt 21:3839-50
11034911
Nature. 2005 May 19;435(7040):365-9
15902261
J Cell Sci. 2006 Jul 15;119(Pt 14):2960-7
16787940
Biophys J. 2006 Apr 1;90(7):2404-13
16415053
Ultramicroscopy. 2008 Dec;109(1):22-31
18790570
J Allergy Clin Immunol. 2006 Jun;117(6):1214-25; quiz 1226
16750977
J Biol Chem. 1983 Mar 10;258(5):3207-14
6826559
Biophys J. 2003 Sep;85(3):2035-43
12944316
Science. 1992 Sep 25;257(5078):1900-5
1411505
J Histochem Cytochem. 1953 Jul;1(4):188-211
13069686
J Allergy Clin Immunol. 1994 Dec;94(6 Pt 2):1103-34
7798550
Pflugers Arch. 2008 Apr;456(1):237-45
18157727
J Cell Biol. 2001 Jun 11;153(6):F25-32
11402057
J Biomech. 2005 Aug;38(8):1685-93
15958226
Ultramicroscopy. 2009 Jun;109(7):775-80
19268460
Curr Opin Struct Biol. 2007 Aug;17(4):437-43
17764925
J Microsc. 2005 Jan;217(Pt 1):109-16
15655068
Ultramicroscopy. 2006 Jun-Jul;106(8-9):663-9
16713093
Ultramicroscopy. 2001 Jan;86(1-2):85-95
11215637
J Exp Med. 1952 Mar;95(3):285-98
14927794
Science. 1989 Mar 24;243(4898):1586-9
2928794
J Cell Biol. 2003 Aug 4;162(3):365-70
12885764
Faraday Discuss Chem Soc. 1986;(81):281-90
3582618
Nat Protoc. 2008;3(7):1132-8
18600218
Nature. 1997 Oct 2;389(6650):509-12
9333242
J Membr Biol. 2001 May 15;181(2):67-76
11420594
Physiology (Bethesda). 2006 Dec;21:430-9
17119156
Biophys J. 1995 May;68(5):1681-6
7612811
Cell Biol Int. 1997 Nov;21(11):685-96
9817809
J Cell Mol Med. 2004 Jan-Mar;8(1):1-21
15090256
Immunopharmacology. 1994 Jan-Feb;27(1):1-11
7515863
Science. 2003 Nov 7;302(5647):1002-5
14605360
J Cell Biol. 1985 Dec;101(6):2145-55
2933414
Biochem Pharmacol. 1981 Jun 15;30(12):1587-94
6268104
Trends Cell Biol. 1999 Oct;9(10):413-7
10481180
Nat Rev Immunol. 2006 Mar;6(3):218-30
16470226
J Struct Biol. 1997 Jul;119(2):84-91
9245747
Nat Nanotechnol. 2008 May;3(5):261-9
18654521
Immunol Cell Biol. 1999 Dec;77(6):499-508
10571670
Genome Biol. 2003;4(11):234
14611651
Science. 1993 May 21;260(5111):1086-94
8493552
J Mol Biol. 2007 Sep 21;372(3):594-607
17686490
J Microsc. 1978 Jan;112(1):115-25
641983
Biochem J. 1986 Dec 1;240(2):313-24
3545184
Science. 1989 Feb 3;243(4891):641-3
2464851
Science. 1993 Jun 4;260(5113):1451-6
17739801
Phys Rev Lett. 1986 Mar 3;56(9):930-933
10033323
J Allergy Clin Immunol. 2001 Jul;108(1):116-21
11447391
Annu Rev Pharmacol Toxicol. 1983;23:331-51
6191653
J Struct Biol. 2000 Jul;131(1):38-43
10945968
Langmuir. 2006 Sep 12;22(19):8151-5
16952255
J Cell Biol. 2006 Nov 6;175(3):477-90
17088428
Angew Chem Int Ed Engl. 2006 Mar 27;45(14):2212-6
16506257
J Cell Sci. 2005 Apr 15;118(Pt 8):1587-94
15784682
Biophys J. 1990 Dec;58(6):1473-80
2275963
J Phys Chem B. 2009 May 7;113(18):6511-9
19366241
Cell. 2004 Aug 6;118(3):363-73
15294161
Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):316-21
8990206
J Cell Biol. 2008 Jun 16;181(6):879-84
18541702
J Cell Biol. 2005 Jul 4;170(1):115-26
15998803
Chem Res Toxicol. 1998 Jul;11(7):730-40
9671535