[Detection of antiadenoviral antibodies by surface plasmon resonance].
A possibility to detect antiadenoviral antibodies by surface plasmon resonance (SPR) was demonstrated. Immobilization on the surface of a sensor of the hexone or degraded purified adenovirus of one of the types (Ad 2) allows finding antibodies to the hexone antigenic determinants of wide specificity common for human adenoviruses of different types. Optimum conditions for immobilization of the antigen and formation of the complex antigen-antibody are determined. The comparative assays of the levels of antibodies in rabbit antisera obtained to the hexone and adenoviruses of different types (1, 2 and 6) by SPR and ELISA was analyzed. The biosensor was used to detect antiadenoviral antibodies in the blood sera of children with aggravation of chronic nonspecific broncho-pulmonary diseases. The sensitivity of SPR in comparison with ELISA was 86.9%, in comparison with the method of fluorescing antibodies (MFA)--89.5%.
Nosach
L M
LM
Boltovets'
P M
PM
Zahorodnia
S D
SD
Povnytsia
O Iu
OIu
Holovan'
A V
AV
Netreba
N I
NI
Dobrochyns'ka
L I
LI
D000260:Adenoviruses, Human / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D036022:Capsid Proteins / Q000276:immunology; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000821:virology; D011817:Rabbits; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus