title: A preliminary study of mesenchymal stem cell-like cells derived from murine corneal stroma.
abstract: BACKGROUND
  Mesenchymal stem cells can be isolated from various tissues besides bone marrow and can differentiate into cells of three germ layers. Recent studies indicate that some cells in corneal stroma express stem cell markers and can also differentiate into chondrocytes and neurocytes. This study was carried out to investigate whether mesenchymal stem cells reside in the murine corneal stroma.
  
  
  METHODS
  Corneas of BALB/c mice were treated with collagenase digestion after the epithelium and endothelium were removed. Then the single cells were harvested and further identified by reverse transcription polymerase chain reaction (RT-PCR). After the immunophenotype of passage 2 corneal stroma-derived cells was analyzed by flow cytometry, attempts were made to differentiate these cells into adipocytes and osteocytes using conditioned medium. Following induction, cells were evaluated by RT-PCR, oil red O and Alizarin Red staining.
  
  
  RESULTS
  Isolated single cells were of stromal origin, not of epithelial or endothelial. Passage 2 corneal stroma-derived cells exhibited the spindle-shaped morphology and expressed CD29, CD90, CD105, and CD71; but were negative for CD34 and CD45. In addition, these cells showed the potentiality of differentiating into adipocytes and osteocytes, which was confirmed by RT-PCR and staining.
  
  
  CONCLUSIONS
  This study demonstrates the presence of mesenchymal stem cell-like cells in the murine corneal stroma. Further analysis of these cells will aid elucidation of the mechanisms of some keratopathies, and these cells may be a source for bioengineering of corneal tissue and for cell-based therapeutics.
authors.affiliation: Department of Ophthalmology, Third Hospital of Hebei Medical University, Ziqiang Road No. 139, Shijiazhuang, China.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Lu
authors.forename: Zhong-You
authors.initials: ZY
authors.lastname: Zhou
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Zhang
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Li
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Wang
authors.forename: Xiu-Jun
authors.initials: XJ
authors.lastname: Song
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D003319:Corneal Stroma / Q000166:cytology*; D005434:Flow Cytometry; D016130:Immunophenotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015703:Antigens, CD; D015415:Biomarkers
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