title: Dithiobis(succinimidyl propionate) modified gold microarray electrode based electrochemical immunosensor for ultrasensitive detection of cortisol.
abstract: Gold microelectrode arrays functionalized with dithiobis(succinimidyl propionate) self-assembled monolayer (SAM) have been used to fabricate an ultrasensitive, disposable, electrochemical cortisol immunosensor. Cortisol specific monoclonal antibody (C-Mab) was covalently immobilized on the surface of gold microelectrode array and the sensors were exposed to solutions with different cortisol concentration. After C-Mab binding, unreacted active groups of DTSP were blocked using ethanol amine (EA) and label-free electrochemical impedance (EIS) technique was used to determine cortisol concentration. EIS results confirmed that EA/C-Mab/DTSP/Au based biosensor can accurately detect cortisol in the range of 1pM-100nM. The biosensor was successfully used for the measurement of cortisol in interstitial fluid in vitro. This research establishes the feasibility of using impedance based biosensor architecture for disposable, wearable cortisol detector.
authors.affiliation: Bio-MEMS and Microsystem Lab, Department of Electrical Engineering, University of South Florida, 4202 E. Fowler Avenue, ENB 118, Tampa, FL 33620, USA. sunilarya333@gmail.com
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Arya
authors.forename: Ganna
authors.initials: G
authors.lastname: Chornokur
authors.forename: Manju
authors.initials: M
authors.lastname: Venugopal
authors.forename: Shekhar
authors.initials: S
authors.lastname: Bhansali
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D007118:Immunoassay / Q000295:instrumentation*; D046228:Microarray Analysis / Q000295:instrumentation*; D008839:Microelectrodes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013388:Succinimides / Q000737:chemistry*
chemical_list: D013388:Succinimides; D006046:Gold; C011240:dithiobis(succinimidylpropionate); D006854:Hydrocortisone
references.citation: Biosens Bioelectron. 2010 Mar 15;25(7):1767-73
references.pmid: 20093001
references.citation: Anal Chem. 1995 Feb 1;67(3):606-12
references.pmid: 7893004
references.citation: Biosens Bioelectron. 2005 Feb 15;20(8):1662-7
references.pmid: 15626625
references.citation: Med Eng Phys. 2006 Dec;28(10):934-43
references.pmid: 16822696
references.citation: Biosens Bioelectron. 2008 Jun 15;23(11):1624-30
references.pmid: 18339536
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Sep 25;824(1-2):51-6
references.pmid: 16054446
references.citation: Anal Chem. 1996 Jan 1;68(1):18-22
references.pmid: 8779431
references.citation: Bioconjug Chem. 2007 Nov-Dec;18(6):1772-7
references.pmid: 17941683
references.citation: Clin Chem. 1987 Sep;33(9):1639-42
references.pmid: 3621564
references.citation: Analyst. 2009 Feb;134(2):380-6
references.pmid: 19173066
references.citation: Anal Bioanal Chem. 2005 Sep;383(2):182-6
references.pmid: 16158300
references.citation: Biosens Bioelectron. 2011 Jan 15;26(5):1847-52
references.pmid: 20153626
references.citation: Biosens Bioelectron. 2008 Apr 15;23(9):1367-73
references.pmid: 18234487
references.citation: Biosens Bioelectron. 2007 Nov 30;23(4):549-55
references.pmid: 17826084
references.citation: Biosens Bioelectron. 2007 Apr 15;22(9-10):2199-207
references.pmid: 17129721
references.citation: Anal Chem. 2008 Dec 1;80(23):9157-61
references.pmid: 19551984
references.citation: Clin Biochem. 2009 Jan;42(1-2):64-71
references.pmid: 18976646
references.citation: Biosens Bioelectron. 2009 Feb 15;24(6):1700-5
references.pmid: 18930651
references.citation: Anal Biochem. 2004 May 15;328(2):113-22
references.pmid: 15113686
references.citation: Clin Chem. 1980 Nov;26(12):1734-7
references.pmid: 7428159
references.citation: Biosens Bioelectron. 2007 Apr 15;22(9-10):2138-44
references.pmid: 17097283
references.citation: Clin Chem. 1999 Sep;45(9):1674-1675
references.pmid: 10471682
references.citation: Biosens Bioelectron. 2007 May 15;22(11):2516-24
references.pmid: 17113279
references.citation: Biosens Bioelectron. 2010 Jan 15;25(5):1095-101
references.pmid: 19926471
references.citation: Biosens Bioelectron. 2008 Sep 15;24(1):78-86
references.pmid: 18455386
references.citation: Clin Chem Lab Med. 2007;45(4):521-5
references.pmid: 17439331
references.citation: Gen Comp Endocrinol. 2007 Oct-Dec;154(1-3):85-90
references.pmid: 17655848
references.citation: Clin Endocrinol (Oxf). 2005 Sep;63(3):336-41
references.pmid: 16117823
references.citation: Anal Chem. 2008 Sep 1;80(17):6747-51
references.pmid: 18656950
references.citation: Biosens Bioelectron. 2007 Apr 15;22(9-10):1955-60
references.pmid: 17000100
references.citation: Biosens Bioelectron. 2004 Jan 15;19(6):563-74
references.pmid: 14683640
references.citation: Biol Psychiatry. 2004 May 1;55(9):940-5
references.pmid: 15110738
references.citation: Talanta. 2005 Mar 15;65(5):1335-42
references.pmid: 18969950
references.citation: Anal Chem. 2007 Jul 1;79(13):4879-86
references.pmid: 17523594
references.citation: Biosens Bioelectron. 2010 Apr 15;25(8):1977-83
references.pmid: 20167466
references.citation: Diabetes Technol Ther. 2006 Apr;8(2):244-52
references.pmid: 16734553
references.citation: Brain Res. 2009 Oct 13;1293:114-20
references.pmid: 19376097
references.citation: J Lab Clin Med. 1997 Oct;130(4):436-41
references.pmid: 9358083
references.citation: Clin Chem. 1997 Aug;43(8 Pt 1):1397-407
references.pmid: 9267320
references.citation: Anal Chem. 2004 Feb 15;76(4):1008-14
references.pmid: 14961732
references.citation: Physiol Behav. 2007 Jan 30;90(1):43-53
references.pmid: 17055006
references.citation: Analyst. 2007 Oct;132(10):1005-9
references.pmid: 17893804