title: [Immune polymorphism analysis of HLA class II antigens in ulcer diseases associated with Helicobacter pylori in children].
abstract: Previous investigations have linked specific HLA class II alleles DRB1 and DQB1 to H. pylori infection (Y. Huang et al., 2005).
  
  
  OBJECTIVE
  to investigate potential contribution of HLA-DRB1 and DQB1 alleles in H. pylori infection susceptibility in a Russian pediatric population.
  
  
  METHODS
  Polymerase chain reaction-sequence specific primer (PCR-SSP) method was used to study the HLA-DRB1, DQB1 allelic frequency distribution in 162 children (93 female) with H. pylori infection was determined by culture, breath test and histology.
  
  
  RESULTS
  The carrier frequency of DQB1*03 was higher among H. pylori--positive patients with chronic gastritis only compared with H. pylori-negative patients. The difference in carrier frequencies for HLA-DRB1*17 was higher in H. pylori-positive ulcer patients compared with an uninfected controls (chi2 = 3.69, p = 0.027). In addition, the frequency of genotypes that possess HLA-DQB1*07 allele in the H. pylori-positive children (with peptic ulcer/ chronic gastritis only) was significantly lower than that in the H. pylori-negative control group.
  
  
  CONCLUSIONS
  HLA-DQB1*07 allele may be associated with protection against H. pylori infection independently of clinical outcome. At the same time, HLA-DRB1*17 allele might be associated with susceptible gene to peptic ulcer formation among H. pylori-positive children.
authors.forename: A A
authors.initials: AA
authors.lastname: Nizhevich
authors.forename: P L
authors.initials: PL
authors.lastname: Shcherbakov
authors.forename: E N
authors.initials: EN
authors.lastname: Akhmadeeva
authors.forename: V U
authors.initials: VU
authors.lastname: Sataev
authors.forename: Z M
authors.initials: ZM
authors.lastname: Elicheva
authors.forename: I Z
authors.initials: IZ
authors.lastname: Usmanova
authors.forename: N P
authors.initials: NP
authors.lastname: Tsyglintseva
mesh_terms: D000293:Adolescent; D000483:Alleles; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D005260:Female; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics* / Q000276:immunology; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D059811:HLA-DRB1 Chains; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D010437:Peptic Ulcer / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D011110:Polymorphism, Genetic
chemical_list: D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains