Electrokinetic lab-on-a-biochip for multi-ligand/multi-analyte biosensing.
We present a simple electrokinetic lab-on-a-biochip (EKLB) with four microchannels integrated with a surface plasmon resonance imaging (iSPR) label-free biosensor that is operated using a single electrical voltage for the simultaneous transport of reagents in all microchannels without conventional fluidic plumbing. We demonstrate the utility of the simple approach with various biosensing experiments, including single injection kinetics (multiple varied ligand densities and single analyte concentration), one shot kinetics (single ligand densities and multiple varied analyte concentrations), and multi-ligand/multianalyte detection. In all cases, the binding kinetics and affinity were extracted using a conventional 1:1 interaction model. Since the reagent transport is done with a single electrical voltage source, scaling up to hundreds to thousands of simultaneous experiments is straightforward.
Krishnamoorthy
Ganeshram
G
BIOS Lab-on-a-Chip Group, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands. g.krishnamoorthy@tnw.utwente.nl
Carlen
Edwin T
ET
deBoer
Hans L
HL
van den Berg
Albert
A
Schasfoort
Richard B M
RB
D015374:Biosensing Techniques / Q000379:methods*; D007700:Kinetics; D008024:Ligands; D046228:Microarray Analysis; D013194:Staining and Labeling; D020349:Surface Plasmon Resonance / Q000379:methods*
D008024:Ligands