title: Hematopoietic activity of human short-term repopulating cells in mobilized peripheral blood cell transplants is restricted to the first 5 months after transplantation.
abstract: Kinetics of hematopoietic recovery driven by different types of human stem and progenitor cells after transplantation are not fully understood. Short-term repopulating cells (STRCs) dominate early hematopoiesis after transplantation. STRCs are highly enriched in adult mobilized peripheral blood compared with cord blood, but the length of their contribution to hematopoiesis remains unclear. To understand posttransplantation durability and lineage contribution of STRCs, we compared repopulation kinetics of mobilized peripheral blood (high STRC content) with cord blood transplants (low STRC content) in long-lived NOD.Cg-Prkdc(scid)Il2rg(tm1Wjl)/SzJ (IL2RG(-/-)) mice. This comparison demonstrates that quantitative contribution of human STRCs to hematopoiesis is restricted to the first 5 months after transplantation. The ratio of STRCs to long-term repopulating cells dramatically changes during ontogeny. This model enables to precisely determine early and late engraftment kinetics of defined human repopulating cell types and to preclinically assess the engraftment kinetics of engineered stem cell transplants.
authors.affiliation: Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center, Heidelberg, Germany.
authors.forename: Oksana
authors.initials: O
authors.lastname: Zavidij
authors.forename: Claudia R
authors.initials: CR
authors.lastname: Ball
authors.forename: Friederike
authors.initials: F
authors.lastname: Herbst
authors.forename: Sylvia
authors.initials: S
authors.lastname: Fessler
authors.forename: Manfred
authors.initials: M
authors.lastname: Schmidt
authors.forename: Christof
authors.initials: C
authors.lastname: von Kalle
authors.forename: Hanno
authors.initials: H
authors.lastname: Glimm
mesh_terms: D000818:Animals; D002455:Cell Division / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D036101:Cord Blood Stem Cell Transplantation; D006085:Graft Survival / Q000502:physiology*; D006410:Hematopoiesis / Q000502:physiology*; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D015375:Receptors, Interleukin-2 / Q000235:genetics; D020127:Recovery of Function / Q000502:physiology; D014183:Transplantation, Heterologous
chemical_list: D015375:Receptors, Interleukin-2