Low-level laser therapy (LLLT) attenuates RhoA mRNA expression in the rat bronchi smooth muscle exposed to tumor necrosis factor-alpha.
Low-level laser therapy (LLLT) has been found to produce anti-inflammatory effects in a variety of disorders. Bronchial smooth muscle (BSM) hyperreactivity is associated with increased Ca+2 sensitivity and increased RhoA mRNA expression. In the current study, we investigated if LLLT could reduce BSM contraction force and RhoA mRNA expression in tumor necrosis factor-alpha (TNF-alpha)-induced BSM hyperreactivity. In the study, 112 male Wistar rats were divided randomly into 16 groups, and BSM was harvested and suspended in TNF-alpha baths for 6 and 24 h, respectively. Irradiation with LLLT was performed with a wavelength of 660 nm for 42 s with a dose of 1.3 J/cm2. This LLLT dose was administered once in the 6-h group and twice in the 24-h group. LLLT significantly decreased contraction force in BSM at 6 h (TNF-alpha + LLLT: 11.65+/-1.10 g/100 mg of tissue) (F=3115) and at 24 h (TNF-alpha+ LLLT: 14.15+/-1.1 g/100 mg of tissue) (F=3245, p<0.05) after TNF-alpha, respectively, when compared to vehicle-bathed groups (control). LLLT also significantly decreased the expression of RhoA mRNA in BSM segments at 6 h (1.22+/-0.20) (F=2820, p<0.05) and 24 h (2.13+/-0.20) (F=3324, p<0.05) when compared to BSM segments incubated with TNF-alpha without LLLT irradiation. We conclude that LLLT administered with this protocol, reduces RhoA mRNA expression and BSM contraction force in TNF-alpha-induced BSM hyperreactivity.
de Lima
Flávia Mafra
FM
Instituto de Pesquisa e Desenvolvimento, IP&D, Av. Shishima Hifumi, 2911, São José dos Campos, CEP: 12244-000 São Paulo, SP, Brazil.
Bjordal
Jan M
JM
Albertini
Regiane
R
Santos
Fábio V
FV
Aimbire
Flavio
F
D000577:Amides / Q000494:pharmacology; D000818:Animals; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D016535:Bronchial Hyperreactivity / Q000139:chemically induced / Q000235:genetics / Q000532:radiotherapy; D020013:Calcium Signaling / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D066298:In Vitro Techniques; D028022:Low-Level Light Therapy; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D011725:Pyridines / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D020742:rhoA GTP-Binding Protein / Q000235:genetics*
D000577:Amides; D011725:Pyridines; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C108830:Y 27632; D020742:rhoA GTP-Binding Protein
Vopr Kurortol Fizioter Lech Fiz Kult. 2006 Mar-Apr;(2):8-10
16752817
J Smooth Muscle Res. 2004 Feb;40(1):25-34
15170075
Science. 1996 Jul 12;273(5272):245-8
8662509
Physiol Rev. 2003 Oct;83(4):1325-58
14506307
J Smooth Muscle Res. 2003 Dec;39(6):221-8
15048014
J Pharmacol Sci. 2004 Aug;95(4):479-82
15297770
Mol Pharmacol. 2000 May;57(5):976-83
10779382
Nature. 1997 Oct 30;389(6654):990-4
9353125
Am J Physiol. 1998 Oct;275(4):L748-55
9755107
Br J Pharmacol. 1999 Jun;127(3):597-600
10401547
Science. 1998 Jun 26;280(5372):2112-4
9641916
J Physiol. 1996 Oct 15;496 ( Pt 2):317-29
8910218
EMBO J. 1996 May 1;15(9):2208-16
8641286
Br J Pharmacol. 1995 Jan;114(1):4-5
7712026
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1995 Jul;111(3):351-7
8564779
Science. 1998 Jun 26;280(5372):2109-11
9641915
Pulm Pharmacol Ther. 2000;13(1):25-9
10718987
Lasers Surg Med. 2006 Sep;38(8):773-8
16868933
J Allergy Clin Immunol. 1991 Oct;88(4):561-71
1918723
Respir Res. 2006 Sep 26;7:121
17002806
J Smooth Muscle Res. 2004 Oct;40(4-5):155-67
15655303
Am J Physiol Lung Cell Mol Physiol. 2008 Jul;295(1):L214-9
18487358
Pflugers Arch. 1990 Sep;417(1):21-8
2127310
Am J Respir Cell Mol Biol. 1999 Jun;20(6):1190-200
10340938
Eur J Pharmacol. 2000 Feb 11;389(1):103-6
10686302
Drugs. 2005;65(10):1355-65
15977968
J Biol Chem. 1999 Dec 24;274(52):37385-90
10601309
Curr Opin Cell Biol. 1991 Feb;3(1):98-104
1854490
Thorax. 2002 Jun;57(6):524-7
12037228
Am J Physiol. 1994 Oct;267(4 Pt 1):L398-405
7943343
Clin Exp Allergy. 1995 Nov;25(11):1038-44
8581835
Br J Pharmacol. 2001 Jul;133(6):886-90
11454662
J Biochem. 2005 Jun;137(6):751-6
16002997
Nature. 1990 Jul 19;346(6281):274-6
2374592
Lasers Med Sci. 2005;20(1):11-20
15965713
Am J Respir Cell Mol Biol. 1992 Oct;7(4):385-92
1382477
Am J Physiol. 1997 Oct;273(4):L775-81
9357852
J Physiol. 1999 Jul 15;518 ( Pt 2):561-9
10381600
Lasers Surg Med. 2009 Jan;41(1):68-74
19143014
Lasers Med Sci. 2009 Jul;24(4):567-76
19005736
Am J Physiol. 1995 May;268(5 Pt 1):L739-46
7762676
J Clin Invest. 1993 Jun;91(6):2673-84
8514874
Res Commun Mol Pathol Pharmacol. 1999;106(1-2):77-85
11127810
J Biol Chem. 2002 Jun 28;277(26):23441-6
11976330