title: Human fibroblasts share immunosuppressive properties with bone marrow mesenchymal stem cells.
abstract: BACKGROUND
  Bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue-derived stem cells share immunosuppressive capacities, suggesting that the latter could be a general property of stromal cells.
  
  
  METHODS
  To check this hypothesis, we compared human BM-MSC and fibroblasts for their in vitro multi-potentiality, expandability and their immunomodulatory properties under normalized optimized culture conditions.
  
  
  RESULTS
  We report that, unlike BM-MSCs, fibroblasts cannot differentiate in vitro into adipocytes and osteoblasts and differ from BM-MSCs by the expression of membrane CD106, CD10 and CD26 and by the expression of collagen VII mRNA. Like BM-MSCs, fibroblasts are unable to provoke in vitro allogeneic reactions, but strongly suppress lymphocyte proliferation induced by allogeneic mixed lymphocyte culture (MLC) or mitogens. We show that fibroblasts' immunosuppressive capacity is independent from prostaglandin E2, IL-10 and the tryptophan catabolising enzyme indoleamine 2,3-dioxygenase and is not abrogated after the depletion of CD8+ T lymphocytes, NK cells and monocytes.
  
  
  CONCLUSIONS
  Finally, fibroblasts and BM-MSCs act at an early stage through blockage of lymphocyte activation, as demonstrated by down-regulation of GZMB (granzyme B) and IL2RA (CD25) expression.
authors.affiliation: Etablissement Français du sang Pyrénées-Méditerranée, Toulouse, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Cappellesso-Fleury
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Puissant-Lubrano
authors.forename: Pol-André
authors.initials: PA
authors.lastname: Apoil
authors.forename: Matthias
authors.initials: M
authors.lastname: Titeux
authors.forename: Peter
authors.initials: P
authors.lastname: Winterton
authors.forename: Louis
authors.initials: L
authors.lastname: Casteilla
authors.forename: Philippe
authors.initials: P
authors.lastname: Bourin
authors.forename: Antoine
authors.initials: A
authors.lastname: Blancher
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D015536:Down-Regulation / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology*; D053804:Granzymes / Q000235:genetics; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000235:genetics; D008213:Lymphocyte Activation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D017154:Stromal Cells / Q000276:immunology
chemical_list: C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D053804:Granzymes
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