IgA detection in human neurocysticercosis using different preparations of heterologous antigen.
Neurocysticercosis (NC) is the most important neurological disease of parasitic origin in humans. IgA and IgG detection in serum from neurocysticercosis patients was tested using some antigenic preparations of total saline extract from Taenia saginata: detergent (D) and aqueous (A) phases extracted with Triton X-114 and the jacalin bound (JBF) and unbound fractions (JUF) obtained by affinity chromatography using jacalin column. Samples were obtained from 45 patients with definitive NC, who were subdivided into active-NC group and inactive-NC group; 35 patients with other parasitoses; and 30 apparently healthy individuals. Sensitivity and specificity were calculated. Specificity to detect IgA and IgG for D phase, respectively, were 89.8% and 86.9% and for IgG detection 91.3% and 76.8% when using D phase and JUF, respectively. D phase and JBF proved to be specific and efficient and could be efficiently utilized as an alternative antigen for IgA detection in NC, with comparable results with IgG.
da S Ribeiro
Vanessa
V
Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará 1720, CEP 38400-902, Uberlândia, Minas Gerais, Brazil.
Manhani
Marianna N
MN
Costa-Cruz
Julia M
JM
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000302:isolation & purification; D015478:Antigens, Heterophile / Q000302:isolation & purification; D002458:Cell Fractionation; D002846:Chromatography, Affinity; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology; D010274:Parasitology / Q000379:methods*; D012680:Sensitivity and Specificity; D041202:Taenia saginata / Q000737:chemistry* / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015478:Antigens, Heterophile; D007070:Immunoglobulin A; D007074:Immunoglobulin G
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