title: Killing effect of interleukin-13 receptor alpha 2 (IL-13Ralpha2) sensitized DC-CTL cells on human glioblastoma U251 cells.
abstract: Recent studies show that IL-13Ralpha2, a brain tumor-associated antigen for IL-13, may play a role in immunotherapy for glioblastoma. Thus, we stimulated the lymphocyte by monocyte-derived dendritic cells (DCs). The DCs were pulsed with IL-13Ralpha2 in vitro and then co-cultured with lymphocytes. After inducing cytotoxic T cells (CTLs) and co-culturing with U251 cells for 24 h in 96 wells, Cell Count Kit-8 (CCK-8) was added to every well equally. The optical density (OD) value was detected and recorded after 2 h. The DCs efficiently presented the antigen to the CTLs, resulting in CTLs activation and proliferation. The induced CTLs showed specific cytotoxic against U251 cells (P<0.01). The results demonstrated that IL-13Ralpha2 induced CTLs could kill glioma U251 in vitro, which suggests that IL-13 Ralpha2 might have such an impact in vivo and thus recombinant IL-13Ra2 protein might be used as an anti-tumor vaccine, providing a promising new strategy for the treatment of brain malignant gliomas.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Xinjiang
authors.initials: X
authors.lastname: Yan
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Su
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhebao
authors.initials: Z
authors.lastname: Wu
authors.forename: Sheng
authors.initials: S
authors.lastname: Ye
authors.forename: Xianghe
authors.initials: X
authors.lastname: Lu
authors.forename: Jinsen
authors.initials: J
authors.lastname: Wu
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
authors.forename: Weiming
authors.initials: W
authors.lastname: Zheng
mesh_terms: D001932:Brain Neoplasms; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D005909:Glioblastoma; D006801:Humans; D053663:Interleukin-13 Receptor alpha2 Subunit / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000276:immunology*
chemical_list: D053663:Interleukin-13 Receptor alpha2 Subunit