Sporamin induce apoptosis in human tongue carcinoma cells by down-regulating Akt/GSK-3 signaling.
We investigated the effects of sporamin, the major soluble protein with a kunitz-type trypsin inhibitory activity in the root tuber of the sweet potato, on cell proliferation, apoptosis, Akt/GSK-3 signaling and its related genes to provide more insights in the mechanism behind the inhibitory effects of sporamin in a human tongue cancer line Tca8113. In this study, sporamin inhibited cell proliferation and induced apoptosis in Tca8113 cells in a concentration-dependent and time-dependent manner. Consistently, Bax was up-regulated and Bcl-2 was down-regulated in sporamin-treated cells. Furthermore, Akt/GSK-3 signaling was down-regulated in sporamin-treated cells. Consistently, the phosphorylated Bad was significantly declined in sporamin-treated Tca8113 cells. These results suggest the antiproliferative effects of sporamin in Tca8113 cells might result partly from induction of apoptosis by down-regulating Akt/GSK-3 pathway.
Yao
Jun
J
School of Medicine, Taizhou University, Jiaojiang District, Taizhou, Zhejiang 318000, China. yaojuntzu@yeah.net
Qian
Cuijuan
C
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D038362:Glycogen Synthase Kinase 3 / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation / Q000187:drug effects; D010940:Plant Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014062:Tongue Neoplasms / Q000188:drug therapy* / Q000473:pathology
D000970:Antineoplastic Agents; D010940:Plant Proteins; C067142:sporamin protein, Ipomoea batatas; D051057:Proto-Oncogene Proteins c-akt; D038362:Glycogen Synthase Kinase 3