title: Effects of increasing days of exposure to prepartum diets on the concentration of certain blood metabolites in dairy cows.
abstract: OBJECTIVE
  To evaluate the effect of the number of days exposure to a prepartum transition diet on mineral, energy and protein metabolism in dairy cows.
  
  
  METHODS
  A prospective cohort study.
  
  
  METHODS
  The prepartum transition diet consisted of ryegrass pasture, cereal hay, grain, grain by-product, protein meals, BioChlor, rumen modifiers, minerals and vitamins and contained 13.9% crude protein, 10.0 MJ metabolisable energy/kg and a dietary cation anion difference of -35 meq/kg dry matter. Forty cows were bled bi-weekly from their introduction to the prepartum transition diet until day 35 of lactation. Blood samples were submitted for estimation of a range of metabolites. Cubic smoothed splines were fitted to scatterplots of metabolite concentration as a function of day relative to calving and the area under the curve (AUC) was calculated. Linear regression modelling determined the effect of days exposed, age, body condition score (BSC) and calving day on AUC. Results The prepartum AUC of blood phosphorus and beta-hydroxybutyrate increased and the AUC of blood calcium and cholesterol decreased with increasing days exposed to the diet. The postpartum AUC of beta-hydroxybutyrate and non-esterified fatty acids decreased with increasing days exposed but increased with days exposed x age and days exposed x BCS, respectively. The AUC of other metabolites did not vary significantly with number of days exposed. Conclusions Increasing exposure to the prepartum diet significantly altered the AUC of blood metabolites associated with mineral, energy and protein metabolism in ways consistent with improvements in production and reproduction previously reported and the result suggests links between energy, protein and skeletal metabolism.
authors.affiliation: Veterinary Clinical Studies, University of Sydney, NSW, Australia.
authors.forename: P J
authors.initials: PJ
authors.lastname: DeGaris
authors.forename: I J
authors.initials: IJ
authors.lastname: Lean
authors.forename: A R
authors.initials: AR
authors.lastname: Rabiee
authors.forename: M A
authors.initials: MA
authors.lastname: Stevenson
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena / Q000502:physiology*; D000818:Animals; D019540:Area Under Curve; D002118:Calcium / Q000097:blood; D002417:Cattle / Q000097:blood* / Q000502:physiology; D002784:Cholesterol / Q000097:blood; D015331:Cohort Studies; D003612:Dairying / Q000379:methods; D004734:Energy Metabolism / Q000502:physiology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D007774:Lactation / Q000097:blood / Q000502:physiology*; D016014:Linear Models; D008903:Minerals / Q000378:metabolism; D010758:Phosphorus / Q000097:blood; D011247:Pregnancy; D011446:Prospective Studies; D011506:Proteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D005230:Fatty Acids, Nonesterified; D008903:Minerals; D011506:Proteins; D010758:Phosphorus; D002784:Cholesterol; D002118:Calcium; D020155:3-Hydroxybutyric Acid