Rapid biocompatibility analysis of materials via in vivo fluorescence imaging of mouse models.
BACKGROUND
Many materials are unsuitable for medical use because of poor biocompatibility. Recently, advances in the high throughput synthesis of biomaterials has significantly increased the number of potential biomaterials, however current biocompatibility analysis methods are slow and require histological analysis.


RESULTS
Here we develop rapid, non-invasive methods for in vivo quantification of the inflammatory response to implanted biomaterials. Materials were placed subcutaneously in an array format and monitored for host responses as per ISO 10993-6: 2001. Host cell activity in response to these materials was imaged kinetically, in vivo using fluorescent whole animal imaging. Data captured using whole animal imaging displayed similar temporal trends in cellular recruitment of phagocytes to the biomaterials compared to histological analysis.


CONCLUSIONS
Histological analysis similarity validates this technique as a novel, rapid approach for screening biocompatibility of implanted materials. Through this technique there exists the possibility to rapidly screen large libraries of polymers in vivo.
Bratlie
Kaitlin M
KM
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
Dang
Tram T
TT
Lyle
Stephen
S
Nahrendorf
Matthias
M
Weissleder
Ralph
R
Langer
Robert
R
Anderson
Daniel G
DG
D000818:Animals; D001672:Biocompatible Materials; D003952:Diagnostic Imaging / Q000379:methods*; D005453:Fluorescence; D008262:Macrophage Activation; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D023421:Models, Animal; D010447:Peptide Hydrolases / Q000378:metabolism; D010586:Phagocytes
D001672:Biocompatible Materials; D010447:Peptide Hydrolases
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