title: Effect of chemical modifications on modulation of gene expression by duplex antigene RNAs that are complementary to non-coding transcripts at gene promoters.
abstract: Antigene RNAs (agRNAs) are small RNA duplexes that target non-coding transcripts rather than mRNA and specifically suppress or activate gene expression in a sequence-dependent manner. For many applications in vivo, it is likely that agRNAs will require chemical modification. We have synthesized agRNAs that contain different classes of chemical modification and have tested their ability to modulate expression of the human progesterone receptor gene. We find that both silencing and activating agRNAs can retain activity after modification. Both guide and passenger strands can be modified and functional agRNAs can contain 2'F-RNA, 2'OMe-RNA, and locked nucleic acid substitutions, or combinations of multiple modifications. The mechanism of agRNA activity appears to be maintained after chemical modification: both native and modified agRNAs modulate recruitment of RNA polymerase II, have the same effect on promoter-derived antisense transcripts, and must be double-stranded. These data demonstrate that agRNA activity is compatible with a wide range of chemical modifications and may facilitate in vivo applications.
authors.affiliation: Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Jonathan K
authors.initials: JK
authors.lastname: Watts
authors.forename: Dongbo
authors.initials: D
authors.lastname: Yu
authors.forename: Klaus
authors.initials: K
authors.lastname: Charisse
authors.forename: Christophe
authors.initials: C
authors.lastname: Montaillier
authors.forename: Pierre
authors.initials: P
authors.lastname: Potier
authors.forename: Muthiah
authors.initials: M
authors.lastname: Manoharan
authors.forename: David R
authors.initials: DR
authors.lastname: Corey
mesh_terms: D045744:Cell Line, Tumor; D020868:Gene Silencing; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism; D016372:RNA, Antisense / Q000096:biosynthesis* / Q000235:genetics; D012330:RNA, Double-Stranded / Q000737:chemistry*; D022661:RNA, Untranslated / Q000096:biosynthesis / Q000235:genetics; D011980:Receptors, Progesterone / Q000096:biosynthesis / Q000235:genetics; D015533:Transcriptional Activation
chemical_list: D009841:Oligonucleotides; D016372:RNA, Antisense; D012330:RNA, Double-Stranded; D022661:RNA, Untranslated; D011980:Receptors, Progesterone; C477371:locked nucleic acid; D012319:RNA Polymerase II
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