microRNA/Argonaute 2 regulates nonsense-mediated messenger RNA decay.
Imperfect base-pairing between microRNA (miRNA) and the 3'-untranslated region of target messenger RNA (mRNA) triggers translational repression of the target mRNA. Here, we provide evidence that human Argonaute 2 targets cap-binding protein (CBP)80/20-bound mRNAs and exon junction complex-bound mRNAs and inhibits nonsense-mediated mRNA decay (NMD), which is restricted tightly to CBP80/20-bound mRNAs. Furthermore, microarray analyses reveal that a subset of cellular transcripts, which are expected to be targeted for NMD, is stabilized by miRNA-mediated gene silencing. The regulation of NMD by miRNAs will shed light on a new post-transcriptional regulation mechanism of gene expression in mammalian cells.
Choe
Junho
J
School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Cho
Hana
H
Lee
Hyung Chul
HC
Kim
Yoon Ki
YK
D020413:3' Untranslated Regions / Q000235:genetics; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D060565:Argonaute Proteins; D018797:Cell Cycle Proteins; D002460:Cell Line; D018389:Codon, Nonsense / Q000235:genetics*; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D039502:Nuclear Cap-Binding Protein Complex / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D020871:RNA Stability / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D034743:RNA-Induced Silencing Complex / Q000378:metabolism
D020413:3' Untranslated Regions; C466422:AGO2 protein, human; D048868:Adaptor Proteins, Signal Transducing; D060565:Argonaute Proteins; D018797:Cell Cycle Proteins; D018389:Codon, Nonsense; C087000:EIF4EBP1 protein, human; D015852:Eukaryotic Initiation Factor-2; C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D039502:Nuclear Cap-Binding Protein Complex; D010750:Phosphoproteins; D012333:RNA, Messenger; D034743:RNA-Induced Silencing Complex
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