title: Effects of unripe Citrus hassaku fruits extract and its flavanone glycosides on blood fluidity.
abstract: The enhancement of blood fluidity may lead to improvements in skin problems resulting from unsmooth circulation or blood stagnation. Since a 50% ethanolic extract (CH-ext) obtained from unripe Citrus hassaku fruits may be a useful ingredient in skin-whitening cosmetics, the present study was designed to examine the effect of CH-ext on blood fluidity. CH-ext concentration-dependently inhibited in vitro collagen-induced rabbit platelet aggregation and in vitro polybrene-induced rat erythrocyte aggregation. The CH-ext showed in vitro fibrinolysis activity in fibrin plate assay. Activity-guided fractionation of the CH-ext using antiplatelet activity, inhibitory activity of erythrocyte aggregation, and fibrinolysis activity revealed that these activities of CH-ext were attributable to naringenin-7-glycoside (prunin). Successive oral administration of CH-ext to rats inhibited the lipopolysaccharide (LPS)-induced decrease of blood platelets and fibrinogen, and LPS-induced increase of fibrin degradation products (FDP) in LPS-induced disseminated intravascular coagulation (DIC) model rats. Effects of CH-ext on blood fluidity were analyzed by a micro channel array flow analyzer (MC-FAN). Preventive oral administration of CH-ext to rats showed dose-dependent reduction of the passage time of whole blood flow of the DIC model rats in comparison with that of the vehicle control rats. These results imply that CH-ext may have effects which improve effects on blood fluidity.
authors.affiliation: Faculty of Pharmacy, Kinki University, Japan.
authors.forename: Kimihisa
authors.initials: K
authors.lastname: Itoh
authors.forename: Megumi
authors.initials: M
authors.lastname: Masuda
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Naruto
authors.forename: Kazuya
authors.initials: K
authors.lastname: Murata
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects; D002957:Citrus / Q000737:chemistry*; D003094:Collagen; D004305:Dose-Response Relationship, Drug; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D005337:Fibrin / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D044950:Flavanones / Q000494:pharmacology*; D005638:Fruit; D006027:Glycosides / Q000494:pharmacology*; D006401:Hematologic Agents / Q000494:pharmacology*; D006403:Hematologic Tests; D018056:Hemorheology / Q000187:drug effects; D008070:Lipopolysaccharides; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
chemical_list: D044950:Flavanones; D006027:Glycosides; D006401:Hematologic Agents; D008070:Lipopolysaccharides; D010936:Plant Extracts; D005337:Fibrin; D005340:Fibrinogen; D003094:Collagen; C005273:naringenin