title: Detection of premature segregation of centromeres in persons exposed to ionizing radiation.
abstract: We have analyzed the frequency of premature centromeric division (PCD) in medical personnel professionally exposed to low doses of radiation. They had chromosome aberrations (CAs) involving dicentric chromosomes, ring chromosomes, acentric fragments, chromosome breaks, and chromatid breaks. The study included 30 exposed subjects and 23 controls who were each analyzed by a conventional cytogenetics procedure and subsequently by fluorescent in situ hybridization (FISH). The latter was applied particularly in order to verify PCD in a specific chromosome (chromosome 18) in both metaphases and interphase nuclei. The results revealed a significant difference (p < 0.001) in frequencies between the two groups (exposed and controls) for all the observed variables (CAs), metaphases with PCD (MPCD), total number of chromosomes with PCD (TPCD), number of PCD metaphases in acrocentric chromosomes (MAPCD), and the total number of acrocentric chromosomes with PCD (TAPCD). The doses of ionizing radiation absorbed by the subjects' bodies were measured with thermoluminescent dosimeters once a month during the duration of occupational exposure. They were expressed in mSv, as mean annual effective doses for the period of exposure. The Spearman rank test showed a high positive correlation between total life effective dose and frequency of CAs and PCD. Based on the results obtained in this study, we suggest that PCD, as a phenomenon manifesting chromosomal instability (CIN), should be considered as a suitable cytogenetic biomarker for individuals occupationally exposed to ionizing radiation.
authors.affiliation: Faculty for Applied Ecology Futura, Singidunum University, 11000 Belgrade, Serbia. jduca@yubc.net
authors.forename: Dubravka
authors.initials: D
authors.lastname: Jovicić
authors.forename: Snezana
authors.initials: S
authors.lastname: Milacić
authors.forename: Tanja D
authors.initials: TD
authors.lastname: Vukov
authors.forename: Boban
authors.initials: B
authors.lastname: Rakić
authors.forename: Milena
authors.initials: M
authors.lastname: Stevanović
authors.forename: Danijela
authors.initials: D
authors.lastname: Drakulić
authors.forename: Rada
authors.initials: R
authors.lastname: Rakić
authors.forename: Nenad
authors.initials: N
authors.lastname: Bukvić
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations / Q000528:radiation effects*; D020090:Chromosome Segregation / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D008297:Male; D008503:Medical Staff / Q000706:statistics & numerical data*; D016273:Occupational Exposure / Q000032:analysis* / Q000706:statistics & numerical data*; D011829:Radiation Dosage; D018570:Risk Assessment