Analysis of the adhesion of Pseudomonas putida NCIB 9816-4 to a silica gel as a model soil using extended DLVO theory.
The extended Derjaguin-Landau-Verwey-Overbeek (DLVO) theory was applied to explain the hydrophobic interaction-mediated adhesion of Pseudomonas putida NCIB 9816-4 to soil. Soil particles are heterogeneous, and it is difficult to define consistent physico-chemical properties such as a contact angle and zeta potential. Hence, a silica gel and a silanized (3-aminopropyltriethoxysilane-coated) silica gel, which showed greater hydrophobicity than the unmodified silica gel, were used as model soils. Gibbs energies for the cell adhesion to the silica gels were calculated with the physico-chemical properties of the microbes and the silica gels and then plotted as a function of the separation distance. The extended DLVO theory successfully explained that the adhesion of P. putida NCIB 9816-4 to the silica gel, a model soil, was primarily caused by hydrophobic interaction.
Hwang
Geelsu
G
Department of Chemical Engineering, Yonsei University, 134 Shinchon-Dong, Seodaemoon-Gu, Seoul 120-749, South Korea.
Lee
Chang-Ha
CH
Ahn
Ik-Sung
IS
Mhin
Byung Jin
BJ
D000465:Algorithms; D000538:Aluminum Silicates; D001422:Bacterial Adhesion / Q000502:physiology*; D018441:Biofilms; D000077215:Clay; D004563:Electrochemistry; D008956:Models, Chemical; D016958:Pseudomonas putida / Q000737:chemistry*; D058428:Silica Gel; D012822:Silicon Dioxide; D012988:Soil Microbiology; D013499:Surface Properties; D014783:Viscosity
D000538:Aluminum Silicates; D058428:Silica Gel; D012822:Silicon Dioxide; D000077215:Clay