Long-term dietary isoflavone exposure enhances estrogen sensitivity of rat uterine responsiveness mediated through estrogen receptor alpha.
The outcome of long-term exposure to dietary isoflavones on estrogen sensitive tissues is discussed controversially. We performed a study on tissue specific effects of lifelong isoflavone exposure on the rat uterus with exposure being initiated prenatally. We compare the effects of the dietary isoflavones, genistein (GEN) and daidzein, or GEN alone to those of isoflavone free diet. Therefore, one group received a phytoestrogen-free diet (PE-free), one an isoflavone-high diet (ISO-high) and one the PE-free diet supplemented with GEN (GEN-rich) throughout their whole lifetime. In ovariectomized adult females a uterotrophic assay was performed, comparing 17beta-estradiol, GEN and two estrogen receptor subtype-specific agonists. The uterus wet weight, the uterine epithelial heights, and uterine markers for proliferation, estrogenicity and estrogen-dependent water channels were determined on mRNA and protein level. The dietary ISO pre-exposure results in a much stronger uterine weight increase following external ERalpha-mediated estrogenic stimuli than seen in the PE-free group. These strongly increased effects were not exclusively due to proliferation hence proliferation associated parameters were almost identical in all groups. Additionally, gene expression analysis showed that estrogen-dependent water channels are highly affected by ISO-containing diets. In conclusion, the lifelong dietary ISO ingestion enhances severely the uterine responsiveness to ERalpha-mediated estrogenic stimuli in female rats. While the uterine proliferation rate was not affected, the water homeostasis was highly affected. Our data clearly demonstrate that estrogen responsiveness is highly modulated by dietary isoflavones. Whether this estrogen sensitivity shift is beneficial or adverse to health remains to be elucidated. However, this is highly relevant for interpreting data from regional differences in endocrine cancer.
Möller
Frank Josef
FJ
Institute for Zoology, Technical University Dresden, 01062 Dresden, Germany. Frank.Moeller@tu-dresden.de
Diel
Patrick
P
Zierau
Oliver
O
Hertrampf
Torsten
T
Maass
Juliane
J
Vollmer
Günter
G
D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics; D051400:Aquaporin 3 / Q000235:genetics; D001835:Body Weight / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000502:physiology*; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D007529:Isoflavones / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D011980:Receptors, Progesterone / Q000235:genetics; D014599:Uterus / Q000187:drug effects*
C496022:Aqp1 protein, rat; C496029:Aqp3 protein, rat; D047628:Estrogen Receptor alpha; D007529:Isoflavones; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D051398:Aquaporin 1; D051400:Aquaporin 3; D004958:Estradiol; C004742:daidzein; D019833:Genistein