Analysis of interaction between tamoxifen and ctDNA in vitro by multi-spectroscopic methods.
Multi-spectroscopic methods including resonance light scattering (RLS), ultraviolet spectra (UV), fluorescence spectra, (1)H NMR spectroscopy, coupled with thermo-denaturation experiments were firstly used to study the interaction of antitumor drug tamoxifen (TMX) with calf thymus (ctDNA) in acetate buffer solutions (pH 4.55). The interaction of TMX with ctDNA could cause a significant enhancement of RLS intensity, the hyperchromic effect, red shift of absorption spectra and the fluorescence quenching of TMX, indicating that there is an inserting interaction between TMX and ctDNA. This inference was confirmed by (1)H NMR spectroscopy. The chemical shift of the benzene proton changes significantly which indicates that TMX could insert into the base pairs of ctDNA. These studies are valuable for a better understanding the mode of TMX-ctDNA interaction further, which are important and useful for designing of new ctDNA targeted drug. And the antitumor drug TMX inserted directly into ctDNA in vitro, which can provide a lot of useful information to explore the development of new and highly effective anti-cancer drugs.
Cai
Changqun
C
College of Chemistry, Xiangtan University, Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, Xiangtan, Hunan 411105, PR China.
Chen
Xiaoming
X
Ge
Fei
F
D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013629:Tamoxifen / Q000494:pharmacology*
D018931:Antineoplastic Agents, Hormonal; D004277:DNA, Single-Stranded; D013629:Tamoxifen; D004247:DNA; C427203:calf thymus DNA