Lack of association of Toll-Like Receptor 2 Arg753Gln with cutaneous leishmaniasis.
The aim of the present study was to investigate the frequency of Arg753Gln and Arg677Trp polymorphisms of TLR2 in patients with cutaneous leishmaniasis (CL) compared to healthy controls. The polymorphisms were genotyped by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system assay (ARMS-PCR). The results showed that the frequency of Arg753Gln genotype was 14.3% and 10.1% in CL patients and normal controls, respectively. No one in either group was homozygous for the mutation. There was no significant difference in the genotype frequency. In contrast to the results for Arg753Gln polymorphism, we did not detect any case with Arg677Trp polymorphism in either control or patient group. In conclusion the TLR2 mutations are found equally in CL patients and healthy subjects.
Ajdary
Soheila
S
Immunology Department, Pasteur Institute of Iran, Tehran, Islamic Republic of Iran. sohary@yahoo.com
Ghamilouie
Mir-Mostafa
MM
Alimohammadian
Mohammad Hossein
MH
Hosseini
Mansoureh
M
Pakzad
Saeed-Reza
SR
D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D002648:Child; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007492:Iran; D016773:Leishmaniasis, Cutaneous / Q000235:genetics* / Q000469:parasitology; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D055815:Young Adult
D051195:Toll-Like Receptor 2