Bacillus marcorestinctum sp. nov., a novel soil acylhomoserine lactone quorum-sensing signal quenching bacterium.
A Gram-positive, facultatively anaerobic, endospore-forming and rod-shaped bacterium was isolated from soil samples and designated strain LQQ. This organism strongly quenches the acylhomoserine lactone quorum-sensing signal. The LQQ strain exhibits phenotypic characteristics consistent with its classification in the genus Bacillus. It is positive in catalase and no special growth factor is needed. It uses glucose as sole carbon source. The DNA G + C content is 39.8 mol %. The closest relatives based on the 16S rRNA gene sequence are Bacillus anthracis, Bacillus thuringiensis, and Brevibacillus brevis (syn. Bacillus brevis) with the similarity of 96.5%. The DNA-DNA hybridization data indicates a low level of genomic relatedness with the relative type strains of Bacillus thuringiensis (6.1%), Bacillus anthracis (10.5%) and Brevibacillus brevis (8.7%). On the basis of the phenotypic and phylogenetic data together with the genomic distinctiveness, the LQQ strain represents a novel species of the genus Bacillus, for which the name Bacillus marcorestinctum sp. nov. is proposed. The type strain is LQQ(T).
Han
Yan
Y
Department of Bio & Food Engineering, Dalian College of Light Industry, Dalian 116034, China.
Chen
Fang
F
Department of Bio & Food Engineering, Dalian College of Light Industry, Dalian 116034, China.
Li
Nuo
N
Department of Bio & Food Engineering, Dalian College of Light Industry, Dalian 116034, China.
Zhu
Bo
B
Department of Bio & Food Engineering, Dalian College of Light Industry, Dalian 116034, China.
Li
Xianzhen
X
Department of Bio & Food Engineering, Dalian College of Light Industry, Dalian 116034, China.
D054742:Acyl-Butyrolactones / Q000378:metabolism*; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D053038:Quorum Sensing; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000378:metabolism; D017423:Sequence Analysis, RNA; D012988:Soil Microbiology
D054742:Acyl-Butyrolactones; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
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