title: [Isolation and determination of activity of IgA1 protease from Neisseria meningitidis].
abstract: A method of the isolation and purification of IgAl protease from a culture of Neisseria meningitidis serogroup A has been developed. Three inactivated intermediates of the production of the meningococcal vaccine, a culture liquid, as well as a supernatant and sediment obtained by the precipitation of bacterial cells by cetavlon, served as a starting material. The purity of IgA1 protease was determined by SDS-PAGE. An immunoenzyme assay for determining the IgA1 protease activity has been devised. The yield of the enzyme with a specific activity of 0.5 to 4 million units/mg from 103 g of the cetavlon precipitate (40 l of culture liquid) was about 600 mug. It was shown that IgAl protease isolated from serogroup A meningococcus is capable of protecting experimental animals (mice) infected with meningococcus of serogroup B.
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Iagudaeva
authors.forename: L S
authors.initials: LS
authors.lastname: Zhigis
authors.forename: O A
authors.initials: OA
authors.lastname: Razguliaeva
authors.forename: V S
authors.initials: VS
authors.lastname: Zueva
authors.forename: E E
authors.initials: EE
authors.lastname: Mel'nikov
authors.forename: V P
authors.initials: VP
authors.lastname: Zubov
authors.forename: L V
authors.initials: LV
authors.lastname: Kozlov
authors.forename: A M
authors.initials: AM
authors.lastname: Bichucher
authors.forename: O V
authors.initials: OV
authors.lastname: Kotel'nikova
authors.forename: A P
authors.initials: AP
authors.lastname: Alliluev
authors.forename: A E
authors.initials: AE
authors.lastname: Avakov
authors.forename: L D
authors.initials: LD
authors.lastname: Rumsh
mesh_terms: D000818:Animals; D007070:Immunoglobulin A / Q000378:metabolism*; D008585:Meningitis, Meningococcal / Q000276:immunology / Q000517:prevention & control; D022401:Meningococcal Vaccines / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009345:Neisseria meningitidis / Q000201:enzymology* / Q000502:physiology; D012697:Serine Endopeptidases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D007070:Immunoglobulin A; D022401:Meningococcal Vaccines; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase