title: Mug28, a meiosis-specific protein of Schizosaccharomyces pombe, regulates spore wall formation.
abstract: The meiosis-specific mug28(+) gene of Schizosaccharomyces pombe encodes a putative RNA-binding protein with three RNA recognition motifs (RRMs). Live observations of meiotic cells that express Mug28 tagged with green fluorescent protein (GFP) revealed that Mug28 is localized in the cytoplasm, and accumulates around the nucleus from metaphase I to anaphase II. Disruption of mug28(+) generated spores with low viability, due to the aberrant formation of the forespore membrane (FSM). Visualization of the FSM in living cells expressing GFP-tagged Psy1, an FSM protein, indicated that mug28Delta cells harbored abnormal FSMs that contained buds, and had a delayed disappearance of Meu14, a leading edge protein. Electron microscopic observation revealed that FSM formation was abnormal in mug28Delta cells, showing bifurcated spore walls that were thicker than the nonbifurcated spore walls of the wild type. Analysis of Mug28 mutants revealed that RRM3, in particular phenylalanin-466, is of primary importance for the proper localization of Mug28, spore viability, and FSM formation. Together, we conclude that Mug28 is essential for the proper maturation of the FSM and the spore wall.
authors.affiliation: Department of Molecular Genetics, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Shigehisa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Okuzaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Kasama
authors.forename: Hideki
authors.initials: H
authors.lastname: Tohda
authors.forename: Aiko
authors.initials: A
authors.lastname: Hirata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nojima
mesh_terms: D020816:Amino Acid Motifs; D019943:Amino Acid Substitution / Q000235:genetics; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002473:Cell Wall / Q000378:metabolism* / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008540:Meiosis; D050505:Mutant Proteins / Q000378:metabolism; D010641:Phenotype; D021381:Protein Transport; D012331:RNA, Fungal / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D012568:Schizosaccharomyces / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism*; D017384:Sequence Deletion / Q000235:genetics; D013172:Spores, Fungal / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors
chemical_list: D018797:Cell Cycle Proteins; C552176:Mug28 protein, S pombe; D050505:Mutant Proteins; D012331:RNA, Fungal; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins
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