Antifreeze protein suppresses spontaneous neural activity and protects neurons from hypothermia/re-warming injury.
Antifreeze proteins (AFP) are associated with protection from freezing. We measured the effect of type I antifreeze protein on spontaneous bursting of mixed neuronal/glial cultures using a multi-electrode array culture system. Antifreeze protein (10mg/ml) reversibly depressed bursting activity without inhibiting mitochondrial oxidative capacity. The effect of antifreeze protein on cold/re-warming injury was investigated in rat hippocampal slice cultures. Compared to bovine serum albumin at a similar concentration, antifreeze protein protected hippocampal neurons from 8h of profound hypothermia at (4 degrees C) followed by re-warming. The protection observed is believed to be associated with the inhibitory effect of antifreeze protein.
Rubinsky
Liel
L
Department of Anesthesia and Perioperative Care, University of California at San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0542, USA. rubinskyl@anesthesia.ucsf.edu
Raichman
Nadav
N
Lavee
Jacob
J
Frenk
Hanan
H
Ben-Jacob
Eshel
E
Bickler
Philip E
PE
D000818:Animals; D021322:Antifreeze Proteins, Type I / Q000494:pharmacology*; D003080:Cold Temperature / Q000009:adverse effects; D005432:Flounder / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology; D007036:Hypothermia, Induced / Q000009:adverse effects*; D007202:Indicators and Reagents; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009415:Nerve Net / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents; D009924:Organ Culture Techniques; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D017694:Rewarming / Q000009:adverse effects*; D013778:Tetrazolium Salts
D021322:Antifreeze Proteins, Type I; D007202:Indicators and Reagents; D018696:Neuroprotective Agents; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide