title: Serum Levels of TGF-beta1, TIMP-1 and TIMP-2 in Patients with Lumbar Spinal Stenosis and Disc Herniation.
abstract: METHODS
  The serum levels of transforming growth factor-beta 1 (TGF-beta1), tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) and TIMP-2 were measured by enzyme-linked immunosorbent assay.
  
  
  OBJECTIVE
  To compare the serum levels of TGF-beta1, TIMP-1 and TIMP-2 between patients with lumbar spinal stenosis and disc herniation.
  
  
  BACKGROUND
  It has been reported that increased concentrations of TGF-beta1, TIMP-1 and TIMP-2 in the ligamentum flavum might be a possible pathogenesis for ligamentum flavum hypertrophy in spinal stenosis. However, it is not determined whether this phenomenon in spinal stenosis is a local or systemic problem.
  
  
  METHODS
  The concentrations of TGF-beta1, TIMP-1 and TIMP-2 were quantitatively analyzed by ELISA in the ligamentum flavum and serum of patients with lumbar spinal stenosis (n=16) and disc herniation (n=16). The thickness of ligamentum flavum was measured on axial T1-weigted magnetic resonance image. The biochemical and radiological results were compared for the two conditions.
  
  
  RESULTS
  The thickness of the ligamentum flavum was larger in patients with spinal stenosis compared with that with disc herniation (p=0.001). The mean concentrations of TGF-beta1, TIMP-1, and TIMP-2 in the ligamentum flavum were significantly higher in patients with spinal stenosis than those with disc herniation (all, p < 0.05). However, the difference in serum levels of TGF-beta1 (p=0.464), TIMP-1 (p=0.146) and TIMP-2 (p=0.794) was not significant between the lumbar spinal stenosis and disc herniation patients.
  
  
  CONCLUSIONS
  Despite increased levels of TGF-beta1, TIMP-1, and TIMP-2 in the ligamentum flavum of spinal stenosis patients compared to disc herniation patients, the serum levels of TGF-beta1, TIMP-1 and TIMP-2 were very similar in both groups. These results indicate that the role of TGF-beta1, TIMP-1 and TIMP-2 on hypertrophy of the ligamentum flavum in spinal stenosis patients is a local phenomenon, not systemic.
authors.affiliation: Department of Orthopedic Surgery, The Catholic University of Korea School of Medicine, Seoul, Korea.
authors.forename: Hyung-Jun
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jong-Beom
authors.initials: JB
authors.lastname: Park
authors.forename: Ho-Yeon
authors.initials: HY
authors.lastname: Won
authors.forename: Han
authors.initials: H
authors.lastname: Chang
references.citation: Eur Spine J. 1999;8(4):323-8
references.pmid: 10483836
references.citation: J Spinal Disord. 1995 Apr;8(2):126-30
references.pmid: 7606119
references.citation: J Hepatol. 2001 Feb;34(2):239-47
references.pmid: 11281552
references.citation: Hepatology. 1991 Aug;14(2):269-73
references.pmid: 1713566
references.citation: Spine (Phila Pa 1976). 2001 Nov 1;26(21):E492-5
references.pmid: 11679833
references.citation: Spine (Phila Pa 1976). 1992 Nov;17(11):1353-60
references.pmid: 1462211
references.citation: Diabetes. 1993 Nov;42(11):1673-7
references.pmid: 8405711
references.citation: Alcohol Clin Exp Res. 1999 May;23(5):940-3
references.pmid: 10371419
references.citation: Am J Pathol. 1993 Jan;142(1):71-8
references.pmid: 8424467
references.citation: Circulation. 1997 Aug 5;96(3):874-81
references.pmid: 9264495
references.citation: Spine (Phila Pa 1976). 1990 Apr;15(4):262-8
references.pmid: 2353270
references.citation: Arch Surg. 1973 Mar;106(3):289-92
references.pmid: 4689802
references.citation: Gastroenterology. 1996 Feb;110(2):576-82
references.pmid: 8566606
references.citation: Am J Pathol. 1998 Dec;153(6):1895-902
references.pmid: 9846979
references.citation: Am J Pathol. 1998 Feb;152(2):485-93
references.pmid: 9466575
references.citation: Biol Chem. 2003 Jun;384(6):863-72
references.pmid: 12887053
references.citation: FEBS Lett. 1992 Feb 17;298(1):29-32
references.pmid: 1544418
references.citation: J Bone Joint Surg Am. 2005 Dec;87(12):2750-2757
references.pmid: 16322626
references.citation: Miner Electrolyte Metab. 1998;24(2-3):111-9
references.pmid: 9525693