title: A fast gas chromatography/mass spectrometry method for the determination of stimulants and narcotics in urine.
abstract: A fast method has been developed for the simultaneous determination of 52 stimulants and narcotics excreted unconjugated in urine by gas chromatography/mass spectrometry (GC/MS). The procedure involves the liquid/liquid extraction of the analytes from urine at strong alkaline pH and the injection of the extract into a GC/MS instrument with a fast GC column (10 m x 0.18 mm i.d.); the short column allows the complete separation of the 52 analytes in a chromatographic run of 8 min. The method has been fully validated giving lower limits of detection (LLODs) satisfactory for its application to antidoping analysis as well as to forensic toxicology. The repeatability of the concentrations and the retention times are good both for intra- and for inter-day experiments (%CV of concentrations always lower than 15 and %CV of retention times lower than 0.6). In addition, the analytical bias is satisfactory (A% always >15%). The method proposed here would be particularly useful whenever there are time constraints and the analyses have to be completed in the shortest possible time.
authors.affiliation: Laboratorio Antidoping, Federazione Medico Sportiva Italiana, Largo G. Onesti 1, 00197 Rome RM, Italy.
authors.forename: Sabina
authors.initials: S
authors.lastname: Strano Rossi
authors.forename: Xavier
authors.initials: X
authors.lastname: de la Torre
authors.forename: Francesco
authors.initials: F
authors.lastname: Botrè
mesh_terms: D000697:Central Nervous System Stimulants / Q000652:urine*; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D009294:Narcotics / Q000652:urine*; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000697:Central Nervous System Stimulants; D009294:Narcotics