Alteration of vaginal elastin metabolism in women with pelvic organ prolapse.
OBJECTIVE
To compare elastin metabolism in the vagina of women with and without pelvic organ prolapse and to define the regulation of this process by hormone therapy (HT).


METHODS
Eighty-seven histologically confirmed full-thickness vaginal biopsies were procured from study participants at time of surgery. Premenopausal women with no prolapse served as controls. Women with prolapse were divided into three groups: premenopausal, postmenopausal not on HT, and postmenopausal on HT. The epithelium was excised leaving the subepithelium, muscularis, and adventitia for analyses. The elastin precursor, tropoelastin, was measured by immunoblotting and mature elastin protein via a desmosine cross-link radioimmunoassay. Matrix metalloproteinases (MMPs)-2 and -9 were quantitated by gelatin zymography. Data were analyzed using Kruskal-Wallis test and post hoc analysis using the Mann-Whitney U test.


RESULTS
Tropoelastin (432%), mature elastin (55%), proMMP-9 (90%), and active MMP-9 (106%) were increased in women with prolapse relative to those in the control group while active MMP-2 (41%) was decreased. Comparison of tropoelastin and mature elastin values obtained from the same women showed them to be independently regulated (r=0.19). Interestingly, the highest amount of both proteins occurred in postmenopausal patients not on HT.


CONCLUSIONS
Elastin metabolism is altered in the vagina of women with prolapse relative to those in the control group, suggesting that vaginal tissue is rapidly remodeling in response to mechanical stretch. We found that elastin levels are highest in the absence of hormones.
Zong
Wenjun
W
From the Department of Obstetrics and Gynecology and Reproductive Sciences, Division of Urogynecology and Reconstructive Pelvic Surgery, and Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania; and Department of Biochemistry, University of Texas at Tyler, Tyler, Texas.
Stein
Suzan E
SE
Starcher
Barry
B
Meyn
Leslie A
LA
Moalli
Pamela A
PA
D000328:Adult; D004549:Elastin / Q000378:metabolism*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008875:Middle Aged; D014333:Tropoelastin / Q000378:metabolism; D014596:Uterine Prolapse / Q000378:metabolism*; D014621:Vagina / Q000737:chemistry*; D055815:Young Adult
D014333:Tropoelastin; D004549:Elastin; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
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