Isolation and characterisation of bacteria from the haloalkaline Lake Elmenteita, Kenya.
Culture-independent studies show that soda lake environments harbour diverse groups of bacteria and archaea. In this study different enrichment and isolation media were used in an attempt to isolate novel groups of bacteria from Lake Elmenteita. Different media were prepared using filter-sterilised water from the lake. The isolates recovered were purified on tryptic soy agar supplemented with 1% sodium carbonate and 4% sodium chloride. Phylogenetic analysis of 181 partial 16S rRNA gene sequences with excellent quality showed that the majority of the isolates were affiliated to the class Gammaproteobacteria and to the genus Bacillus. Isolates from the genus Halomonas and Bacillus constituted 37 and 31% of the total sequenced isolates, respectively. Other groups recovered were related to Marinospirillum, Idiomarina, Vibrio, Enterococcus, Alkalimonas, Alkalibacterium, Amphibacillus, Marinilactibacillus and the actinobacteria Nocardiopsis and Streptomyces. Fifty-one different genera were represented with 31 and 15 cultures scoring with their nearest neighbour similarities below 98 and 97%, respectively. Some novel taxa were identified which had not been isolated previously from the soda environment. The results show that the use of different media with varying compositions can help retrieve novel bacterial diversity from the soda lake environment.
Mwirichia
Romano
R
Institute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000, Nairobi, Kenya. romano.mwirichia@yahoo.com
Muigai
A W
AW
Tindall
B
B
Boga
H I
HI
Stackebrandt
E
E
D001419:Bacteria / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*; D001483:Base Sequence; D017931:DNA Primers; D005618:Fresh Water; D007630:Kenya; D010802:Phylogeny; D014871:Water Microbiology
D017931:DNA Primers
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1711-1714
16014506
C R Acad Hebd Seances Acad Sci D. 1965 Dec 8;261(23):5243-6
4954820
Appl Environ Microbiol. 1985 Oct;50(4):781-90
16346911
Int J Syst Evol Microbiol. 2007 Nov;57(Pt 11):2489-2492
17978206
Int J Syst Evol Microbiol. 2000 Mar;50 Pt 2:901-907
10758902
Oecologia. 1978 Jan;32(1):11-35
28308664
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2859-65
19060072
Extremophiles. 1999 May;3(2):97-102
10356995
Extremophiles. 1998 Aug;2(3):359-66
9783184
Int J Syst Evol Microbiol. 2003 May;53(Pt 3):711-720
12807191
Mol Biol Evol. 2007 Aug;24(8):1596-9
17488738
Mikrobiologiia. 2001 Nov-Dec;70(6):825-37
11785140
Int J Syst Bacteriol. 1990 Jan;40(1):92-7
2223602
Proc Natl Acad Sci U S A. 2004 Jul 27;101(30):11030-5
15258291
Curr Microbiol. 2010 Jan;60(1):47-52
19806396
Adv Microb Physiol. 1983;24:173-214
6364726
Glycobiology. 1991 Jun;1(3):257-63
1794038
Plant Physiol. 2000 Apr;122(4):1239-47
10759521
J Bacteriol. 1986 Sep;167(3):766-73
3745119
Appl Environ Microbiol. 2001 Sep;67(9):4293-304
11526036
FASEB J. 1993 Jan;7(1):113-23
8422957
Int J Syst Evol Microbiol. 2009 Jan;59(Pt 1):53-9
19126723
Extremophiles. 2004 Feb;8(1):63-71
15064991
Antonie Van Leeuwenhoek. 1997 Jul;72(1):3-19
9296259
Antonie Van Leeuwenhoek. 2006 Apr-May;89(3-4):395-403
16622791
Extremophiles. 2004 Jun;8(3):193-200
14986177
Syst Appl Microbiol. 2009 Sep;32(6):371-8
19625151
Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1723-1728
15388735
Int J Syst Bacteriol. 1997 Jul;47(3):788-94
9226911
Evolution. 1985 Jul;39(4):783-791
28561359
Extremophiles. 1998 Aug;2(3):191-200
9783165
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712