Comparative analysis of rabies virus isolates from Brazilian canids and bats based on the G gene and G-L intergenic region.
Rabies virus (RABV) isolates from two species of canids and three species of bats were analyzed by comparing the C-terminal region of the G gene and the G-L intergenic region of the virus genome. Intercluster identities for the genetic sequences of the isolates showed both regions to be poorly conserved. Phylogenetic trees were generated by the neighbor-joining and maximum parsimony methods, and the results were found to agree between the two methods for both regions. Putative amino acid sequences obtained from the G gene were also analyzed, and genetic markers were identified. Our results suggest that different genetic lineages of RABV are adapted to different animal species in Brazil.
Carnieli
Pedro
P
Pasteur Institute, Av. Paulista 393, São Paulo, SP, Brazil. pedrocarnielijunior@ymail.com
Fahl
Willian de Oliveira
Wde O
Brandão
Paulo Eduardo
PE
Oliveira
Rafael de Novaes
Rde N
Macedo
Carla Isabel
CI
Durymanova
Ekaterina
E
Castilho
Juliana Galera
JG
D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild / Q000821:virology; D000956:Antigens, Viral / Q000235:genetics*; D001938:Brazil / Q000453:epidemiology; D046028:Canidae / Q000821:virology*; D002685:Chiroptera / Q000821:virology*; D021901:DNA, Intergenic / Q000235:genetics*; D005814:Genes, Viral; D014644:Genetic Variation; D006023:Glycoproteins / Q000235:genetics*; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011818:Rabies / Q000453:epidemiology / Q000662:veterinary*; D011820:Rabies virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*
D000956:Antigens, Viral; D021901:DNA, Intergenic; D006023:Glycoproteins; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus