Dendrigraft poly-L-lysine: a non-immunogenic synthetic carrier for antibody production.
An easily synthesized DendriGraft poly-lysine DGL-G3 (third generation) was shown to act as an efficient carrier for raising antibodies directed against small molecules. The immunological properties of three different forms of DGL-G3 were investigated: the native form (molecular weight 22 kDa bearing a mean number of 123 surface amino groups as TFA salts), a form modified at the C-terminus by fluorescein (fluorescein-DGL-G3), and last a surface-modified form bearing histamine (DGL-G3-Histamine). Our studies demonstrate the native DGL-G3 to be inefficient in eliciting antibody production in rabbits. Immunizations of rabbits using the core-modified fluorescein-DGL-G3 or the surface-modified DGL-G3-histamine conjugate failed in eliciting antibody production. Conversely, following a primary immunization using a BSA-histamine conjugate, a second immunization with DGL-G3-histamine conjugate improved the production of specific hapten-directed antibodies, which demonstrates the utility of DGL-G3 as a carrier for the production of highly specific antibody against haptens.
Romestand
Bernard
B
IFREMER, Université Montpellier 2, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5119, Ecosystèmes Lagunaires, place E. Bataillon, CC80, 34095 Montpellier cedex 5, France.
Rolland
Jean-Luc
JL
Commeyras
Auguste
A
Coussot
Gaëlle
G
Desvignes
Isabelle
I
Pascal
Robert
R
Vandenabeele-Trambouze
Odile
O
D000818:Animals; D000917:Antibody Formation; D004337:Drug Carriers; D005260:Female; D011107:Polylysine / Q000008:administration & dosage*; D011817:Rabbits
D004337:Drug Carriers; D011107:Polylysine