Gold nanoparticle-fluorophore complexes: sensitive and discerning "noses" for biosystems sensing.
Gold nanoparticles (NPs) efficiently quench adsorbed fluorophores. Upon disruption of such complexes by an analyte, fluorescence turn-on is observed. By judicious choice of the functionalized NP and the fluorophore, these complexes display different responses to analytes, thus leading to versatile yet simple array-based sensor platforms. Using this strategy, we can identify proteins in buffer and serum, distinguish between both different species and different strains of bacteria, and differentiate between healthy, cancerous, and metastatic human and murine cells.
Bunz
Uwe H F
UH
School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332, USA. uwe.bunz@chemistry.gatech.edu
Rotello
Vincent M
VM
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D015374:Biosensing Techniques; D011756:Diphosphates / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D046228:Microarray Analysis; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry
D011756:Diphosphates; D005456:Fluorescent Dyes; D011108:Polymers; D011506:Proteins; D006046:Gold