Scattered light fluorescence microscopy: imaging through turbid layers.
A major limitation of any type of microscope is the penetration depth in turbid tissue. Here, we demonstrate a fundamentally novel kind of fluorescence microscope that images through optically thick turbid layers. The microscope uses scattered light, rather than light propagating along a straight path, for imaging with subwavelength resolution. Our method uses constructive interference to focus scattered laser light through the turbid layer. Microscopic fluorescent structures behind the layer were imaged by raster scanning the focus.
Vellekoop
Ivo M
IM
Physik Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland. ivo.vellekoop@physik.uzh.ch
Aegerter
Christof M
CM
D007368:Interferometry; D008027:Light; D008856:Microscopy, Fluorescence / Q000379:methods*; D012542:Scattering, Radiation