Single-molecule force spectroscopy approach to enzyme catalysis.
Enzyme catalysis has been traditionally studied using a diverse set of techniques such as bulk biochemistry, x-ray crystallography, and NMR. Recently, single-molecule force spectroscopy by atomic force microscopy has been used as a new tool to study the catalytic properties of an enzyme. In this approach, a mechanical force ranging up to hundreds of piconewtons is applied to the substrate of an enzymatic reaction, altering the conformational energy of the substrate-enzyme interactions during catalysis. From these measurements, the force dependence of an enzymatic reaction can be determined. The force dependence provides valuable new information about the dynamics of enzyme catalysis with sub-angstrom resolution, a feat unmatched by any other current technique. To date, single-molecule force spectroscopy has been applied to gain insight into the reduction of disulfide bonds by different enzymes of the thioredoxin family. This minireview aims to present a perspective on this new approach to study enzyme catalysis and to summarize the results that have already been obtained from it. Finally, the specific requirements that must be fulfilled to apply this new methodology to any other enzyme will be discussed.
Alegre-Cebollada
Jorge
J
Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
Perez-Jimenez
Raul
R
Kosuri
Pallav
P
Fernandez
Julio M
JM
D001703:Biophysics / Q000379:methods; D002384:Catalysis; D004220:Disulfides / Q000737:chemistry; D004798:Enzymes / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force / Q000379:methods*; D008968:Molecular Conformation; D010100:Oxygen / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013879:Thioredoxins / Q000737:chemistry
D004220:Disulfides; D004798:Enzymes; D011506:Proteins; D013438:Sulfhydryl Compounds; D013879:Thioredoxins; D010100:Oxygen
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