Dynamics of augmented soil system containing biphenyl with Dyellaginsengisoli LA-4.
One high efficient biphenyl-degrading strain Dyella ginsengisoli LA-4 was inoculated into biphenyl-contaminated soil for bioaugmentation in this study. The results showed that bioaugmentation could accelerate the startup period of the biphenyl bioremediation process compared with the non-augmented one. PCR-DGGE fingerprints demonstrated that both of the diversity and pattern of microbial community were affected by the addition of strain LA-4 and biphenyl. Biphenyl-utilizing populations gradually increased and become the dominant species. The introduced strain LA-4 could be persistent and co-exist well with the indigenous populations. However, both of the strain LA-4 and indigenous microorganisms in the bioaugmented system would be partially inhibited by Zn(2+) and Ni(2+). This study suggests that it is feasible and potentially useful to remediate biphenyl-contaminated soil using bioaugmentation with D. ginsengisoli LA-4.
Zhao
Li-jun
LJ
School of Chemical Engineering, China University of Petroleum, Beijing 102249, China. zlj@cup.edu.cn
Jia
Yu-hong
YH
Zhou
Ji-ti
JT
Li
Ang
A
Chen
Jin-fu
JF
D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000737:chemistry*; D016000:Cluster Analysis; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D016172:DNA Fingerprinting; D004586:Electrophoresis; D052918:Environmental Restoration and Remediation / Q000379:methods*; D008670:Metals / Q000737:chemistry; D011153:Population; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry*; D044166:Xanthomonadaceae / Q000235:genetics / Q000378:metabolism*
D001713:Biphenyl Compounds; D008670:Metals; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D004247:DNA