title: Visual detection of pandemic influenza A H1N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye.
abstract: A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of pandemic influenza A H1N1 virus infection. The reaction was performed in one step in a single tube at 65 degrees C for 60 min with the addition of hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was approximately 60 copies, and no cross-detection was observed. The assay was evaluated further with 50 clinical specimens diagnosed clinically with seasonal influenza or pandemic influenza A H1N1 virus infection. RT-LAMP with HNB dye was demonstrated to be a sensitive and easy assay for rapid detection of pandemic influenza A H1N1 virus.
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100, Xuan Wu District, Beijing 100052, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Ma
authors.forename: Yue-Long
authors.initials: YL
authors.lastname: Shu
authors.forename: Kai
authors.initials: K
authors.lastname: Nie
authors.forename: Meng
authors.initials: M
authors.lastname: Qin
authors.forename: Da-Yan
authors.initials: DY
authors.lastname: Wang
authors.forename: Rong-Bao
authors.initials: RB
authors.lastname: Gao
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.forename: Le-Ying
authors.initials: LY
authors.lastname: Wen
authors.forename: Feng
authors.initials: F
authors.lastname: Han
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: Zhou
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Cheng
authors.forename: De-Xin
authors.initials: DX
authors.lastname: Li
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Dong
mesh_terms: D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D009282:Naphthalenesulfonates / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*; D014773:Virology / Q000379:methods*
chemical_list: D009282:Naphthalenesulfonates; C540250:trisodium 3-hydroxy-4-((2Z)-2-(2-oxo-4-sulfonatonaphthalen-1-ylidene)hydrazinyl)naphthalene-2,7-disulfonate