title: Functional modules in the Arabidopsis core cell cycle binary protein-protein interaction network.
abstract: As in other eukaryotes, cell division in plants is highly conserved and regulated by cyclin-dependent kinases (CDKs) that are themselves predominantly regulated at the posttranscriptional level by their association with proteins such as cyclins. Although over the last years the knowledge of the plant cell cycle has considerably increased, little is known on the assembly and regulation of the different CDK complexes. To map protein-protein interactions between core cell cycle proteins of Arabidopsis thaliana, a binary protein-protein interactome network was generated using two complementary high-throughput interaction assays, yeast two-hybrid and bimolecular fluorescence complementation. Pairwise interactions among 58 core cell cycle proteins were tested, resulting in 357 interactions, of which 293 have not been reported before. Integration of the binary interaction results with cell cycle phase-dependent expression information and localization data allowed the construction of a dynamic interaction network. The obtained interaction map constitutes a framework for further in-depth analysis of the cell cycle machinery.
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium.
authors.forename: Joanna
authors.initials: J
authors.lastname: Boruc
authors.forename: Hilde
authors.initials: H
authors.lastname: Van den Daele
authors.forename: Jens
authors.initials: J
authors.lastname: Hollunder
authors.forename: Stephane
authors.initials: S
authors.lastname: Rombauts
authors.forename: Evelien
authors.initials: E
authors.lastname: Mylle
authors.forename: Pierre
authors.initials: P
authors.lastname: Hilson
authors.forename: Dirk
authors.initials: D
authors.lastname: Inzé
authors.forename: Lieven
authors.initials: L
authors.lastname: De Veylder
authors.forename: Eugenia
authors.initials: E
authors.lastname: Russinova
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002453:Cell Cycle; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D030562:Databases, Protein; D020411:Oligonucleotide Array Sequence Analysis; D025941:Protein Interaction Mapping; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D018844:Cyclin-Dependent Kinases
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