Functional studies on RNA-transfected cell microarrays.
RNA-transfected cell microarray shows great promise in functional genomics. By printing siRNA complexed with transfection reagent on glass slides, arrays of transfected cells are formed in which the phenotypic consequences of gene suppression can be investigated. Using reporter plasmids with fluorescence intensity as output data, we have developed a strategy for statistical analysis of the intensity data from medium-scale functional studies using data from several experimental replicates.
Fjeldbo
Christina Saeten
CS
Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
Misund
Kristine
K
Günther
Clara-Cecilie
CC
Langaas
Mette
M
Steigedal
Tonje Strømmen
TS
Thommesen
Liv
L
Laegreid
Astrid
A
Bruland
Torunn
T
D002460:Cell Line; D006801:Humans; D016014:Linear Models; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D034741:RNA, Small Interfering / Q000378:metabolism*; D012996:Solutions; D013223:Statistics as Topic; D016707:Tissue Fixation; D014162:Transfection / Q000379:methods*
D034741:RNA, Small Interfering; D012996:Solutions