Expression pattern of testis-specific expressed gene 2 in cryptorchidism model and its role in apoptosis of spermatogenic cells.
In our previous study, we identified a novel testis-specific expressed gene 2 (TSEG-2) from mouse testis. To further investigate its functions, 35 male Balb/c mice (8 weeks old) were divided into cryptorchidism group (n=20), sham group (n=10), and control group (n=5). In cryptorchidism group, the right testes were anchored to the inner lateral abdominal wall. In situ hybridization (ISH) was applied to measure the localization of TSEG-2 in mouse testis. Real-time quantitative PCR was performed to detect the expression of TSEG-2 gene. Meanwhile, under the mediation of polyethylenimine (PEI), the recombinant vector pEGFP-TSEG-2 (n=5) or empty vector (mock, n=5) was transfected into the testis of male mice. The transfection efficiencies were measured under a fluorescence microscope. The apoptosis of spermatogenic cells was detected by terminal deoxynuleotidyl-mediated nick end labeling (TUNEL). The results showed that TSEG-2 was expressed in convoluted seminiferous tubules, more precisely, in spermatogonia and spermatocytes. As compared with sham and control groups, the TSEG-2 transcription was significantly enhanced (P<0.05) and was correlated with apoptosis of spermatogenic cells in cryptorchid testes (P<0.05). PEI was efficient in mediating transfection of TSEG-2 into seminiferous tubules of testis. One week post-transfection, intratesticular injection of TSEG-2 resulted in increased apoptosis of spermatogenic cells in vivo (P<0.05). These results indicate that TSEG-2 may participate in the apoptosis of spermatogenic cells and the pathogenesis of cryptorchidism.
Hu
Tao
T
Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. hutao19771212@163.com
Wang
Zhiyu
Z
Zeng
Fuqing
F
Chen
Xiaochun
X
Gu
Zhaohui
Z
Zheng
Liduan
L
Tong
Qiangsong
Q
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D003456:Cryptorchidism / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism*; D013091:Spermatogenesis / Q000235:genetics*; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism*
Biol Reprod. 2001 Jun;64(6):1583-9
11369582
Asian J Androl. 2006 Jan;8(1):53-9
16372119
Biol Reprod. 2001 Jul;65(1):229-39
11420244
Nat Rev Drug Discov. 2005 Jul;4(7):581-93
16052241
Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7297-301
7638184
Gene Ther. 2000 Mar;7(5):401-7
10694822
Hum Gene Ther. 2003 Sep 1;14(13):1215-23
12952593
Int J Dev Biol. 1997 Feb;41(1):111-22
9074943
Biol Reprod. 2003 Jul;69(1):37-47
12606389
Mol Reprod Dev. 2001 Jul;59(3):285-93
11424214
J Androl. 1991 Sep-Oct;12(5):335-43
1684973
J Biomed Mater Res. 2000 Sep 5;51(3):321-8
10880073
Science. 2002 Dec 6;298(5600):1999-2002
12471261
Zhonghua Nan Ke Xue. 2009 Feb;15(2):99-105
19323366
Asian J Androl. 2003 Jun;5(2):83-8
12778315
Acta Paediatr. 2007 May;96(5):611-6
17462053