title: [Genetic analysis of HAV strains isolated from patients with acute hepatitis in Hebei Shijiazhuang of China].
abstract: OBJECTIVE
  To characterize the hepatitis A virus (HAV) wild type strains circulating in Hebei Shijiazhuang of China during 2005-2007, to provide the bases for further investigation of the sources of HAV infection.
  
  
  METHODS
  The VP1/P2A junction regions were detected by RT-PCR from HAV IgM positives serum samples during 2005 and 2007, the 34 RT-PCR positive samples were sequenced and subjected to phylogenetic analysis by Neighbor Joining (NJ) method.
  
  
  RESULTS
  All the detected HAV strains were identified as sub-genotype I A, the homology of nucleotide sequence in the VP1-2A imation region ranged from 95%-100%, the amino acid sequences of HAV strains almost had no difference.
  
  
  CONCLUSIONS
  There are different HAV strains existing in Hebei Shijiazhuang of China, same HAV strain may exist in different areas; or in one area, identical or different HAV strains may be detected. This work provides the bases for further investigation of the sources of HAV infection and also for effectively control measures to prevent the spread of the disease.
authors.affiliation: Department of Laboratory Medicine, the Fifth Hospital of Shijiazhuang, Hebei 050021, China.
authors.forename: Er-hei
authors.initials: EH
authors.lastname: Dai
authors.forename: Zi-jie
authors.initials: ZJ
authors.lastname: Guo
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Lu
authors.forename: Yu-zhen
authors.initials: YZ
authors.lastname: Lu
authors.forename: Jun-liang
authors.initials: JL
authors.lastname: Hou
authors.forename: Zhi-chen
authors.initials: ZC
authors.lastname: Cao
authors.forename: Qing-ling
authors.initials: QL
authors.lastname: Meng
authors.forename: Jing-yuan
authors.initials: JY
authors.lastname: Cao
authors.forename: Sheng-li
authors.initials: SL
authors.lastname: Bi
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D002648:Child; D002681:China; D005260:Female; D006506:Hepatitis A / Q000821:virology*; D017957:Hepatitis A Virus, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D015678:Viral Structural Proteins / Q000235:genetics; D055815:Young Adult
chemical_list: C096539:VP1 protein, hepatitis A virus; D015678:Viral Structural Proteins