title: Metabolic engineering of glyoxalase pathway for enhancing stress tolerance in plants.
abstract: Glyoxalase system consists of two enzymes glyoxalase I (Gly I) and glyoxalase II (Gly II). Gly I detoxifies methylglyoxal (MG), a cytotoxic byproduct of glycolysis, to S-lactoylglutathione (SLG) where it uses one molecule of reduced glutathione. Subsequently, SLG is converted to lactate by Gly II and one molecule of reduced glutathione is recycled back into the system. The level of MG, which is produced ubiquitously in all living organisms, is enhanced upon exposure to different abiotic stresses in plants. Overexpression of glyoxalase pathway genes in transgenic plants has been found to keep a check on the MG level under stress conditions, regulate glutathione homeostasis, and the transgenic plants are able to survive and grow under various abiotic stresses.
authors.affiliation: Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
authors.forename: Ananda
authors.initials: A
authors.lastname: Mustafiz
authors.forename: Khirod K
authors.initials: KK
authors.lastname: Sahoo
authors.forename: Sneh L
authors.initials: SL
authors.lastname: Singla-Pareek
authors.forename: Sudhir K
authors.initials: SK
authors.lastname: Sopory
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D057075:Enzyme Assays; D035264:Flowers / Q000187:drug effects / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005818:Genetic Engineering / Q000379:methods*; D018525:Germination / Q000187:drug effects; D007791:Lactoylglutathione Lyase / Q000235:genetics* / Q000302:isolation & purification; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D012275:Oryza / Q000187:drug effects / Q000235:genetics; D010944:Plants / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D030821:Plants, Genetically Modified; D011765:Pyruvaldehyde / Q000378:metabolism; D012231:Rhizobium / Q000187:drug effects / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects; D013869:Thiolester Hydrolases / Q000235:genetics* / Q000302:isolation & purification; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics; D014170:Transformation, Genetic / Q000187:drug effects; D000099301:Hydroxyacylglutathione Hydrolase
chemical_list: D007791:Lactoylglutathione Lyase; D011765:Pyruvaldehyde; D012965:Sodium Chloride; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase