title: [Cerebral lymphatic blockage aggravates apoptosis of cortical neurons after subarachnoid hemorrhage in rats in vivo].
abstract: This present study was performed to investigate the influence of cerebral lymphatic blockage (CLB) on apoptosis of cortical neurons after subarachnoid hemorrhage (SAH) in rats in vivo. Healthy adult Wistar rats were randomly divided into normal control group, SAH group and SAH+CLB group. SAH model was made by double injection of autologous blood into the cisterna magna. On the third day after the second cisternal injection, morphological changes of cortical cells were observed by hematoxylin-eosin (HE) combined with propidium iodide (PI) staining. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method was applied to determine in situ apoptosis in the cerebral cortex. Immunohistochemistry was conducted to detect the expression of Caspase-3 and Bcl-2 in cortical neurons. HE and PI staining showed that cortical neurons of SAH rats were partly shrinkable; the nuclei showed wavy, folded or wrinkled appearance, and some nuclei had the shape of crescent. The cortical neurons in SAH+CLB group distributed sparsely and the nuclear fragmentation, apoptotic bodies were found, surrounded by the formation of vacuoles. The numbers of TUNEL-positive cells in SAH group and SAH+CLB group were higher than that in the normal control group, while the number in SAH+CLB group was significantly higher than that in the SAH group. Caspase-3 expressions in SAH group and SAH+CLB group were higher than that in the normal control group, while the expression in SAH+CLB group was significantly higher than that in the SAH group. Bcl-2 expressions in SAH group and SAH+CLB group were higher than that in the normal control group, while the expression in the SAH+CLB group was significantly lower than that in SAH group. The results obtained suggest that CLB exacerbates the apoptosis of cortical neurons in rats after SAH by up-regulating Caspase-3 expression and down-regulating Bcl-2 expression.
authors.affiliation: Key Laboratory of Cerebral Microcirculation, University of Shandong, Taishan Medical College, Department of Neurology, Affiliated Hospital of Taishan Medical College, Taian 271000, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Gao
authors.forename: Bing
authors.initials: B
authors.lastname: Gao
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Jia
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Yang
authors.forename: Yan-Bo
authors.initials: YB
authors.lastname: Zhang
authors.forename: Bao-Liang
authors.initials: BL
authors.lastname: Sun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D002540:Cerebral Cortex / Q000473:pathology*; D005260:Female; D042601:Lymphatic Vessels / Q000293:injuries* / Q000473:pathology; D008297:Male; D009474:Neurons / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013345:Subarachnoid Hemorrhage / Q000473:pathology / Q000503:physiopathology*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; C505410:Casp3 protein, rat; D053148:Caspase 3