Characterization of virulence factors and genetic background of Staphylococcus aureus isolated from Peking University People's Hospital between 2005 and 2009.
The aim of this study was to investigate both the genetic features of MRSA strains and the occurrence of virulence factors produced by Staphylococcus aureus strains isolated from Peking University People's Hospital in Beijing, China, between 2005 and 2009. A total of 179 S. aureus strains were isolated, 139 of which were MRSA. The MRSA strains were characterized epidemiologically by SCCmec typing, spa typing and agr typing, then were classified into different genetic groups. The prevalence of genes coding for 14 exotoxins and eight adhesion factors among the S. aureus samples was assessed via polymerase chain reaction. Cluster analysis based on virulence factors-encoding gene content was performed to divide the MRSA isolates into valid clusters. Correspondence analysis was done to analyze the correlation between virulence factors clusters and genetic groups. JCSC1716-agrI-t030 (67.6%), SCCmec-IIIA-agrI-t030 (14.4%), SCCmec-IIIA-agrI-t037 (8.6%) and SCCmecII-agrII-t002 (2.2%) were four predominant MRSA clones. PVL was positive only in MSSA strains, there were at least three superantigenic toxins in our HA-MRSA clones, the prevalence of 16 virulence factors genes (sea, seb, sec, sed, seg, sei, sej, pvl, lukE-lukD, eta, bbp, can, ebp, clfA, fib, fnbB) in MRSA and MSSA was found to be significantly different from MSSA (P < 0.05). Results of correspondence analysis among clusters based on virulence factors genes and groups based on genetic typing illustrated not only the correspondence relationship between groups and clusters overall (P < 0.001), but also the genetic diversity of MRSA strains with respect to virulence factors genes.
Liu
Min
M
Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China.
Liu
Jingbo
J
Guo
Yu
Y
Zhang
Zheng
Z
D018829:Adhesins, Bacterial / Q000032:analysis / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism; D015373:Bacterial Typing Techniques; D002681:China / Q000453:epidemiology; D003428:Cross Infection / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005098:Exotoxins / Q000032:analysis / Q000235:genetics; D005787:Gene Frequency; D006761:Hospitals; D006785:Hospitals, University; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005098:Exotoxins; D037521:Virulence Factors
J Clin Microbiol. 2003 Dec;41(12):5442-8
14662923
J Clin Microbiol. 2006 Mar;44(3):847-53
16517865
Diagn Microbiol Infect Dis. 2008 Oct;62(2):226-9
18653301
J Clin Microbiol. 2007 Jan;45(1):127-33
17093021
J Clin Microbiol. 2008 Feb;46(2):574-81
18032612
Diagn Microbiol Infect Dis. 2009 Oct;65(2):116-22
19748421
Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):14130-5
18772392
Infect Genet Evol. 2009 Sep;9(5):941-7
19559820
Antimicrob Agents Chemother. 2002 Jul;46(7):2155-61
12069968
Trends Microbiol. 1998 Dec;6(12):484-8
10036727
Clin Microbiol Infect. 2008 Apr;14(4):381-4
18190580
J Hosp Infect. 2008 Jan;68(1):32-8
18069084
J Bacteriol. 2004 Jul;186(14):4665-84
15231800
Clin Microbiol Infect. 2009 Dec;15(12):1139-45
19392889
FEMS Microbiol Lett. 1998 Nov 15;168(2):227-33
9835033
Epidemiol Infect. 1994 Oct;113(2):259-66
7925664
J Clin Microbiol. 2002 Nov;40(11):4060-7
12409375
J Bacteriol. 2005 Dec;187(24):8312-21
16321935
Infect Immun. 2002 Feb;70(2):631-41
11796592
J Hosp Infect. 2007 Jun;65 Suppl 2:105-9
17540252
J Clin Microbiol. 2003 Sep;41(9):4465-7
12958296
Eur J Clin Microbiol Infect Dis. 2003 May;22(5):306-9
12743832
BMC Microbiol. 2007 Oct 29;7:98
17967176
Int J Infect Dis. 2006 Nov;10(6):482-3
16935539
J Med Microbiol. 2008 Apr;57(Pt 4):403-410
18349356
Clin Microbiol Infect. 2007 Sep;13(9):843-5
17686137