title: Detection of quinone oxidoreductase 1 (NQO1) single-nucleotide polymorphisms (SNP) related to benzene metabolism in immortalized B lymphocytes from a Chinese Han population.
abstract: Single-nucleotide polymorphisms (SNP) in genes coding metabolizing enzymes modulate gene functions and cellular toxicity in response to chemicals. Quinone oxidoreductase 1 (NQO1) is an important detoxification enzyme involved in the catabolism of 1,4-benzoquinone (1,4-BQ), a benzene metabolite believed to be associated with bone-marrow toxicity and leukemia. Gene function was evaluated in immortalized human B lymphocytes derived from a Chinese Han population with independent genotypes at 2 NQO1 SNP sites. 1,4-Benzoquinone was incubated with these immortalized lymphocytes of differing genotypes. Among the genotypes of 2 SNP examined, cell lines with rs1800566CC showed a higher NQO1 enzymic activity after a 48 h of treatment with 10 muM 1,4-BQ, and a lower comet rate compared with cells of CT/TT genotypes. Data suggested that NQO1 rs1800566 might serve as a functional genetic marker for benzene toxicity in the Chinese Han population. The immortalized B lymphocytes derived from different populations might thus be used as a biomarker to detect functional genetic markers related to exposure to environmental chemicals.
authors.affiliation: Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.forename: Lihong
authors.initials: L
authors.lastname: Yin
authors.forename: Geyu
authors.initials: G
authors.lastname: Liang
authors.forename: Ran
authors.initials: R
authors.lastname: Liu
authors.forename: Yuepu
authors.initials: Y
authors.lastname: Pu
mesh_terms: D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000201:enzymology* / Q000378:metabolism; D001554:Benzene / Q000378:metabolism* / Q000633:toxicity; D016227:Benzoquinones / Q000378:metabolism / Q000633:toxicity; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002681:China / Q000208:ethnology; D004249:DNA Damage; D016172:DNA Fingerprinting; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000378:metabolism; D055815:Young Adult
chemical_list: D016227:Benzoquinones; D012333:RNA, Messenger; C004532:quinone; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human; D001554:Benzene