A new label-free continuous fluorometric assay for trypsin and inhibitor screening with tetraphenylethene compounds.
A new label-free continuous assay with the ensemble of compound 1 and Arg(6) for trypsin and inhibitor screening was successfully developed by taking advantage of the aggregation-induced emission feature of tetraphenylethene compounds. Both CLSM and DLS studies confirm the formation of the aggregation complex of compound 1 and Arg(6) and disassembly after further addition of trypsin.
Xue
Wangxin
W
Beijing National Laboratory for Molecular Sciences, Organic Solids Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Zhang
Guanxin
G
Zhang
Deqing
D
Zhu
Daoben
D
D004353:Drug Evaluation, Preclinical; D005453:Fluorescence; D005470:Fluorometry / Q000379:methods*; D015394:Molecular Structure; D013267:Stilbenes / Q000737:chemistry* / Q000494:pharmacology; D014357:Trypsin / Q000737:chemistry* / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000737:chemistry* / Q000494:pharmacology
D013267:Stilbenes; D014361:Trypsin Inhibitors; D014357:Trypsin