Characterization of a wheat-Thinopyrum bessarabicum (T2JS-2BS.2BL) translocation line.
Thinopyrum bessarabicum (2n = 2x = 14, JJ or E(b)E(b)) is an important genetic resource for wheat improvement due to its salinity tolerance and disease resistance. Development of wheat-Th. bessarabicum translocation lines will facilitate its practical utilization in wheat improvement. In this study, a novel wheat-Th. bessarabicum translocation line T2JS-2BS.2BL, which carries a segment of Th. bessarabicum chromosome arm 2JS was identified and further characterized using sequential chromosome C-banding, genomic in situ hybridization (GISH), dual-color fluorescent in situ hybridization (FISH) and DNA markers. The translocation breakpoint was mapped within bin C-2BS1-0.53 of chromosome 2B through marker analysis. Compared to the Chinese Spring (CS) parent and to CS-type lines, the translocation line has more fertile spikes per plant, longer spikes, more grains per spike and higher yield per plant, which suggests that the alien segment carries yield-related genes. However, plants with the translocation are also taller, head later and have lower 1,000-kernel weight than CS or CS-type lines. By using markers specific to the barley photoperiod response gene Ppd-H1, it was determined that the late heading date was conferred by a recessive allele located on the 2JS segment. In addition, four markers specific for the translocated segment were identified, which can be used for marker-aided screening.
Qi
Zengjun
Z
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. zjqi@njau.edu.cn
Du
Pei
P
Qian
Baoli
B
Zhuang
Lifang
L
Chen
Huafeng
H
Chen
Tingting
T
Shen
Jian
J
Guo
Jie
J
Feng
Yigao
Y
Pei
Ziyou
Z
D002871:Chromosome Banding; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D002523:Edible Grain / Q000235:genetics / Q000254:growth & development*; D005819:Genetic Markers / Q000235:genetics; D006823:Hybrid Vigor; D017404:In Situ Hybridization, Fluorescence; D006109:Poaceae / Q000235:genetics*; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
D005819:Genetic Markers
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