title: Expression of ammonia transporter family members, Rh B glycoprotein and Rh C glycoprotein, in the developing rat kidney.
abstract: Ammonia metabolism is a primary component of acid-base homeostasis but is incompletely developed at time of birth. Rh B glycoprotein (Rhbg) and Rh C glycoprotein (Rhcg) are recently recognized ammonia transporter family members expressed in the mammalian kidney. This study's purpose was to establish the expression and localization of Rhbg and Rhcg during kidney development. We examined kidneys from fetal days 16 (E16), 18 (E18), and 20 (E20), and from the first 21 days of postnatal development. Rhbg was expressed initially at E18, with expression only in the connecting tubule (CNT); at E20, Rhbg was expressed in both the CNT and the medullary collecting duct (MCD). In contrast, Rhcg was first expressed at E16 with basal expression in the ureteric bud; at E18, it was expressed in a subset of CNT cells with an apical pattern, followed by apical and basolateral expression in the MCD at E20. In the cortex, Rhbg and Rhcg expression increased in the CNT before expression in the cortical collecting duct during fetal development. In the MCD, both Rhbg and Rhcg expression was initially in cells in the papillary tip, with gradual removal from the tip during the late fetal period and transition during the early neonatal period to an adult pattern with predominant expression in the outer MCD and only rare expression in cells in the initial inner MCD. Double-labeling with intercalated cell-specific markers identified that Rhbg and Rhcg were expressed initially in CNT cells, CNT A-type intercalated cells and non-A, non-B intercalated cells, and in MCD A-type intercalated cells. We conclude that expression of Rhbg and Rhcg parallels intercalated cell development and that immature Rhbg and Rhcg expression at birth contributes to incomplete ammonia excretion capacity.
authors.affiliation: Department of Anatomy, Ewha Womans University, Seoul, Korea.
authors.forename: Ki-Hwan
authors.initials: KH
authors.lastname: Han
authors.forename: Su-Youn
authors.initials: SY
authors.lastname: Lee
authors.forename: Wan-Young
authors.initials: WY
authors.lastname: Kim
authors.forename: Jung-A
authors.initials: JA
authors.lastname: Shin
authors.forename: Jin
authors.initials: J
authors.lastname: Kim
authors.forename: I David
authors.initials: ID
authors.lastname: Weiner
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D015415:Biomarkers / Q000378:metabolism; D027682:Cation Transport Proteins / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D007150:Immunohistochemistry; D007668:Kidney / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats
chemical_list: D015415:Biomarkers; D027682:Cation Transport Proteins; D008562:Membrane Glycoproteins; C508142:Rh type B glycoprotein, rat; C508143:Rh type C glycoprotein, rat; D000641:Ammonia
references.citation: Gastroenterology. 2003 May;124(5):1432-40
references.pmid: 12730882
references.citation: J Biol Chem. 2000 Aug 18;275(33):25641-51
references.pmid: 10852913
references.citation: Am J Physiol Gastrointest Liver Physiol. 2005 May;288(5):G1036-47
references.pmid: 15576624
references.citation: Proc R Soc Lond B Biol Sci. 1953 Sep;141(905):489-97
references.pmid: 13100406
references.citation: Am J Physiol Renal Physiol. 2006 Feb;290(2):F397-408
references.pmid: 16144966
references.citation: Biochem J. 1969 Aug;114(1):65-70
references.pmid: 5810067
references.citation: Pediatrics. 1948 Sep;2(3):290-302
references.pmid: 18880099
references.citation: EMBO J. 1998 Aug 10;17(5):1236-47
references.pmid: 9482721
references.citation: Adv Enzyme Regul. 1968;6:227-42
references.pmid: 5720337
references.citation: Am J Physiol. 1987 Oct;253(4 Pt 2):F595-605
references.pmid: 3310662
references.citation: Am J Physiol Renal Physiol. 2004 Nov;287(5):F878-85
references.pmid: 15226152
references.citation: Am J Physiol Renal Physiol. 2007 Oct;293(4):F1238-47
references.pmid: 17652373
references.citation: Biochem J. 1972 Nov;130(1):23P-24P
references.pmid: 4347787
references.citation: Am J Physiol. 1975 Mar;228(3):693-8
references.pmid: 1115233
references.citation: Am J Physiol Renal Physiol. 2006 Jun;290(6):F1443-52
references.pmid: 16434569
references.citation: J Am Soc Nephrol. 1991 May;1(11):1193-203
references.pmid: 1932632
references.citation: Development. 2006 Dec;133(23):4737-48
references.pmid: 17079272
references.citation: Biochem J. 1967 Jun;103(3):744-8
references.pmid: 6049392
references.citation: Am J Physiol Renal Physiol. 2005 Aug;289(2):F347-58
references.pmid: 15798090
references.citation: J Appl Physiol. 1968 Feb;24(2):135-41
references.pmid: 5637674
references.citation: Nature. 1959 Jan 10;183(4654):115-6
references.pmid: 13622711
references.citation: Pflugers Arch. 2004 Apr;448(1):16-28
references.pmid: 14758480
references.citation: Transfus Clin Biol. 2006 Mar-Apr;13(1-2):159-63
references.pmid: 16564723
references.citation: Development. 2003 May;130(9):2013-25
references.pmid: 12642503
references.citation: Nature. 2008 Nov 20;456(7220):339-43
references.pmid: 19020613
references.citation: Acta Paediatr (Stockh). 1961 Nov;50:603-16
references.pmid: 13905336
references.citation: J Biol Chem. 1997 May 23;272(21):13725-30
references.pmid: 9153225
references.citation: Am J Physiol Renal Physiol. 2009 Jun;296(6):F1364-75
references.pmid: 19321595
references.citation: Am J Physiol. 1994 Jun;266(6 Pt 2):F977-90
references.pmid: 8023977
references.citation: J Am Soc Nephrol. 2003 Mar;14(3):545-54
references.pmid: 12595489
references.citation: Acta Paediatr Scand. 1969 Jul;58(4):321-9
references.pmid: 5387933
references.citation: Biochem Biophys Res Commun. 1968 Aug 13;32(3):426-32
references.pmid: 4969950
references.citation: J Am Soc Nephrol. 2007 Oct;18(10):2672-82
references.pmid: 17855646
references.citation: Am J Physiol. 1970 May;218(5):1394-8
references.pmid: 5438267
references.citation: J Am Soc Nephrol. 2006 Oct;17(10):2670-9
references.pmid: 16928804
references.citation: Am J Physiol Regul Integr Comp Physiol. 2000 Apr;278(4):R931-6
references.pmid: 10749781
references.citation: Biochem Genet. 1999 Apr;37(3-4):119-38
references.pmid: 10495887
references.citation: J Physiol. 1954 May 28;124(2):358-69
references.pmid: 13175137
references.citation: Nat Genet. 2000 Nov;26(3):341-4
references.pmid: 11062476
references.citation: Nephron Exp Nephrol. 2008;110(2):e49-58
references.pmid: 18776723
references.citation: J Am Soc Nephrol. 2002 Aug;13(8):1999-2008
references.pmid: 12138130
references.citation: Am J Physiol Renal Physiol. 2004 Oct;287(4):F628-38
references.pmid: 15149971
references.citation: Kidney Int. 1981 Sep;20(3):353-8
references.pmid: 7300125
references.citation: Curr Opin Nephrol Hypertens. 2004 Sep;13(5):533-40
references.pmid: 15300160
references.citation: Am J Physiol Renal Physiol. 2003 Feb;284(2):F323-37
references.pmid: 12388412
references.citation: Am J Physiol Renal Physiol. 2009 Mar;296(3):F543-55
references.pmid: 19129254
references.citation: Am J Physiol Renal Physiol. 2007 Jan;292(1):F269-77
references.pmid: 16926446
references.citation: Annu Rev Physiol. 2007;69:317-40
references.pmid: 17002591
references.citation: Am J Physiol. 1996 Apr;270(4 Pt 2):F575-92
references.pmid: 8967336
references.citation: Am J Physiol Renal Physiol. 2007 Oct;293(4):F1342-54
references.pmid: 17686949
references.citation: J Clin Invest. 2004 Jun;113(11):1560-70
references.pmid: 15173882
references.citation: J Biol Chem. 2001 Jan 12;276(2):1424-33
references.pmid: 11024028
references.citation: Am J Physiol. 1971 Jan;220(1):213-7
references.pmid: 5538655
references.citation: Clin Chem. 1996 Nov;42(11):1855-60
references.pmid: 8906088
references.citation: Am J Physiol Lung Cell Mol Physiol. 2009 Jul;297(1):L153-63
references.pmid: 19429772
references.citation: Nephron Physiol. 2003;93(3):p76-86
references.pmid: 12660494
references.citation: Kidney Int Suppl. 1991 Jul;33:S95-102
references.pmid: 1890804