[Extraction, selenium-nanoparticle preparation and anti-virus bioactivity determination of polysaccharides from Caulerpa taxifolia].
OBJECTIVE
To extract polysaccharides from Caulerpa taxifolia and determine its anti-virus bioactivity.


METHODS
The crude polysaccharides were extracted by hot water and precipitated with ethanol, then they were further purified by DEAE-Cellulose and Sephadex G-200 chromatography. After determined by chemicophysical analysis, the polished polysaccharides were selenium treated and bio-assayed by MT and CPE methods.


RESULTS
The crude extraction from Caulerpa taxifolia was a kind of sulfated polysaccharides in which the average content of polysaccharides and sulfate were 27. 9% and 11.5%, respectively. The recovery rate of the polished polysaccharides was 66.3% after the 2-column purification. The IC50 and TI of the purified polysaccharide SCpl1 were 2.2 mg/mL and 3057.0, respectively. After treated with selenium, the average diameter of the nanoparticle was 28.6 nm, and its bioactivity and TI index were enhanced significantly although with a higher cytotoxicity.


CONCLUSIONS
The polysaccharides from Caulerpa taxifolia and its selenium particles was a kind of bioactive substance for anti-virus drug-candidate development.
Men
Xiao-yuan
XY
Department of PET-CT, Tianjin Cancer Hospital & Institute, Tianjin Medical University/Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, China.
Xu
Wen-gui
WG
Zhu
Xiang
X
Ma
Wen-chao
WC
D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology; D046068:Caulerpa / Q000737:chemistry*; D029822:Enterovirus B, Human / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D049329:Nanostructures; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012643:Selenium / Q000737:chemistry* / Q000494:pharmacology; D013678:Technology, Pharmaceutical / Q000379:methods*
D000998:Antiviral Agents; D011134:Polysaccharides; D012643:Selenium