title: Chemical changes during fermentation of Abhayarishta and its standardization by HPLC-DAD.
abstract: Abhayarishta is an Ayurvedic formulation prepared traditionally by the fermentation of the decoction of Terminalia chebula (pericarp), Vitis vinifera (fruits), Embelia ribes (fruits) and Madhuca indica (flowers). In the present communication, chemical changes occurring during fermentation in Abhayarishta have been studied for the purpose of its standardization. An HPLC-DAD method for quantitative estimation of selected marker constituents in the formulation has been developed and validated. A comparison of decoction and final processed formulation revealed that major polyphenolics (chebulagic and chebulinic acid) of T. chebula were hydrolyzed to their respective monomers and, consequently, there was an increase in the amount of chebulic acid, gallic acid, ellagic acid and ethyl gallate after fermentation. 5-Hydroxymethyl furfural (5-HMF) was also found in the formulation. Thus, emphasis is laid upon consideration of processing methods of formulation which has been lacking in the standardization of most of Ayurvedic formulations.
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, Punjab, India.
authors.forename: Uma Ranjan
authors.initials: UR
authors.lastname: Lal
authors.forename: Shailendra Mani
authors.initials: SM
authors.lastname: Tripathi
authors.forename: Sanjay M
authors.initials: SM
authors.lastname: Jachak
authors.forename: Kamlesh Kumar
authors.initials: KK
authors.lastname: Bhutani
authors.forename: Inder Pal
authors.initials: IP
authors.lastname: Singh
mesh_terms: D001578:Benzopyrans / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004610:Ellagic Acid / Q000032:analysis; D005285:Fermentation; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000032:analysis; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D047348:Hydrolyzable Tannins / Q000378:metabolism*; D008515:Medicine, Ayurvedic; D010936:Plant Extracts / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D001578:Benzopyrans; D005960:Glucosides; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; C103481:chebulinic acid; D004610:Ellagic Acid; C076178:chebulagic acid; C048734:ethyl gallate; D005707:Gallic Acid; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde