Degradation of nicotine in tobacco waste extract by newly isolated Pseudomonas sp. ZUTSKD.
The newly isolated Pseudomonas sp. ZUTSKD was evaluated for its ability to degrade nicotine in tobacco waste extract (TWE). The strain degraded nicotine completely when the concentration of reducing sugar in TWE was lower than 8 gL(-1). Yeast extract and phosphate additions improved nicotine degradation in 5% TWE. At 30 degrees C and pH 7.0, with additional 15 gL(-1) Na(2)HPO(4).6H(2)O and 6 gL(-1) KH(2)PO(4) in 5% TWE, Pseudomonas sp. ZUTSKD could degrade 97% of nicotine (1.6 gL(-1)) in 12h. The data showed that strain ZUTSKD could be useful to control the nicotine content in TWE.
Zhong
Weihong
W
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, PR China. whzhong@zjut.edu.cn
Zhu
Chenjing
C
Shu
Ming
M
Sun
Kedan
K
Zhao
Lei
L
Wang
Chang
C
Ye
Zhijuan
Z
Chen
Jianmeng
J
D001673:Biodegradation, Environmental; D007220:Industrial Waste / Q000517:prevention & control*; D009538:Nicotine / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D013045:Species Specificity; D014026:Nicotiana / Q000382:microbiology*
D007220:Industrial Waste; D009538:Nicotine