PsbS-specific zeaxanthin-independent changes in fluorescence emission spectrum as a signature of energy-dependent non-photochemical quenching in higher plants.
The PsbS protein of photosystem II is necessary for the development of energy-dependent quenching of chlorophyll (Chl) fluorescence (qE), and PsbS-deficient Arabidopsis plant leaves failed to show qE-specific changes in the steady-state 77 K fluorescence emission spectra observed in wild-type leaves. The difference spectrum between the quenched and un-quenched states showed a negative peak at 682 nm. Although the level of qE development in the zeaxanthin-less npq1-2 mutant plants, which lacked violaxanthin de-epoxidase enzyme, was only half that of wild type, there were no noticeable changes in this qE-dependent difference spectrum. This zeaxanthin-independent DeltaF682 signal was not dependent on state transition, and the signal was not due to photobleaching of pigments either. These results suggest that DeltaF682 signal is formed due to PsbS-specific conformational changes in the quenching site of qE and is a new signature of qE generation in higher plants.
Zulfugarov
Ismayil S
IS
Department of Molecular Biology, Pusan National University, Busan, 609-735, Korea. iszulfugarov@pusan.ac.kr
Tovuu
Altanzaya
A
Dogsom
Bolormaa
B
Lee
Chung Yeol
CY
Lee
Choon-Hwan
CH
D029681:Arabidopsis Proteins / Q000235:genetics; D005453:Fluorescence; D045342:Light-Harvesting Protein Complexes / Q000235:genetics; D009154:Mutation; D010777:Photochemistry; D045332:Photosystem II Protein Complex / Q000235:genetics; D013050:Spectrometry, Fluorescence / Q000379:methods; D024341:Xanthophylls / Q000737:chemistry*; D065146:Zeaxanthins
D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes; C489731:NPQ4 protein, Arabidopsis; D045332:Photosystem II Protein Complex; D024341:Xanthophylls; D065146:Zeaxanthins