Mycoplasma mucosicanis sp. nov., isolated from the mucosa of dogs.
Fourteen Mycoplasma strains were isolated from the oral cavity and genital tract of asymptomatic dogs. Isolates had been preliminarily identified by conventional serological testing as Mycoplasma bovigenitalium, but in 16S-23S rRNA intergenic spacer PCR-RFLP assays the isolates exhibited an RFLP pattern distinct from M. bovigenitalium PG11(T). Analysis of the 16S rRNA gene placed a representative of the isolates (strain 1642(T)) in the M. bovigenitalium subcluster of the Mycoplasma bovis cluster of mycoplasmas, with the highest sequence similarities to Mycoplasma californicum ST-6(T) (96.4 %), M. bovigenitalium PG11(T) (96.3 %) and Mycoplasma phocirhinis 852(T) (96.2 %). 16S rRNA gene sequence similarities almost equidistant from three recognized species and results obtained by sequence analysis of the 16S-23S rRNA intergenic spacer region, polar lipid profiles and serological reactions indicated that this organism represents a novel species of the genus Mycoplasma for which the name Mycoplasma mucosicanis sp. nov. is proposed, with strain 1642(T) ( = ATCC BAA-1895(T)  = DSM 22457(T)) as the type strain.
Spergser
Joachim
J
Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
Langer
Stefan
S
Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
Muck
Simone
S
Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
Macher
Kathrin
K
Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
Szostak
Michael
M
Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
Rosengarten
Renate
R
Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
Busse
Hans-Jürgen
HJ
Institute of Microbiology and Genetics, University of Vienna, A-1030 Vienna, Austria.|Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
D000818:Animals; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer; D004285:Dogs; D005835:Genitalia / Q000382:microbiology; D008969:Molecular Sequence Data; D058889:Molecular Typing; D009055:Mouth / Q000382:microbiology; D009092:Mucous Membrane / Q000382:microbiology*; D009174:Mycoplasma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012703:Serotyping
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S