title: Heterogeneity of Leishmania infantum chagasi kinetoplast DNA in Teresina (Brazil).
abstract: Leishmania infantum chagasi is the causative agent of visceral leishmaniasis in Brazil. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of kinetoplast DNA (kDNA) minicircles was used to evaluate genetic profiles of 48 Leishmania infantum chagasi strains from dog and human parasite cultures, fresh collected dog bone marrow aspirates, and from infected sand flies. Results revealed that heterogeneity in kDNA minicircles depends mostly on the source of the samples, with cultured parasites showing a high degree of homogeneity.
authors.affiliation: Department of Parasitology, Bioscience Institute, Sao Paulo State University, Botucatu, Brazil (UNESP).
authors.forename: Diego Peres
authors.initials: DP
authors.lastname: Alonso
authors.forename: Dorcas Lamounier
authors.initials: DL
authors.lastname: Costa
authors.forename: Ivete Lopes
authors.initials: IL
authors.lastname: de Mendonça
authors.forename: Carlos Henrique Nery
authors.initials: CH
authors.lastname: Costa
authors.forename: Paulo Eduardo Martins
authors.initials: PE
authors.lastname: Ribolla
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D018105:DNA, Kinetoplast / Q000235:genetics*; D004285:Dogs; D014644:Genetic Variation; D006801:Humans; D018314:Leishmania infantum / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000453:epidemiology / Q000469:parasitology*; D010802:Phylogeny
chemical_list: D018105:DNA, Kinetoplast