Local release of highly loaded antibodies from functionalized nanoporous support for cancer immunotherapy.
We report that antibodies can be spontaneously loaded in functionalized mesoporous silica (FMS) with superhigh density (0.4-0.8 mg of antibody/mg of FMS) due to their comprehensive noncovalent interaction. The superhigh loading density and noncovalent interaction between FMS and antibodies allow long-lasting local release of the immunoregulatory molecules from FMS under physiological conditions. Preliminary data indicate that FMS-anti-CTLA4 antibody injected directly into a mouse melanoma induces much greater and extended inhibition of tumor growth than the antibody given systemically. Our findings open up a novel approach for local delivery of therapeutically active proteins to tumors and, potentially, other diseases.
Lei
Chenghong
C
Pacific Northwest National Laboratory, Richland, Washington 99352, USA. chenghong.lei@pnl.gov
Liu
Pu
P
Chen
Baowei
B
Mao
Yumeng
Y
Engelmann
Heather
H
Shin
Yongsoon
Y
Jaffar
Jade
J
Hellstrom
Ingegerd
I
Liu
Jun
J
Hellstrom
Karl Erik
KE
D000818:Animals; D055910:Antibodies, Immobilized / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use*; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000627:therapeutic use; D007167:Immunotherapy; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D009369:Neoplasms / Q000628:therapy*; D016062:Porosity; D051381:Rats; D012822:Silicon Dioxide / Q000737:chemistry
D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D012822:Silicon Dioxide