title: Hypoxia during pregnancy in rats leads to the changes of the cerebral white matter in adult offspring.
abstract: The aim of the present study is to evaluate the effect of reduced fetal oxygen supply on cerebral white matter in the adult offspring and further assess its susceptibility to postnatal hypoxia and high-fat diet. Based on a 3 x 2 full factorial design consisting of three factors of maternal hypoxia, postnatal high-fat diet, and postnatal hypoxia, the ultrastructure of myelin, axon and capillaries were observed, and the expression of myelin basic protein (MBP), neurofilament-H+L(NF-H+L), and glial fibrillary acidic protein (GFAP) was analyzed in periventricular white matter of 16-month-old offspring. Demyelination, injured axon and damaged microvasculars were observed in maternal hypoxia offspring. The main effect of maternal hypoxia lead to decreased expression of MBP or NF-H+L, and increased expression of GFAP (all P<0.05). Moreover, there was positive three-way interaction among maternal hypoxia, high-fat diet and postnatal hypoxia on MBP, NF-H+L or GFAP expression (all P<0.05). In summary, our results indicated that maternal hypoxia during pregnancy in rats lead to changes of periventricular white matter in adult offspring, including demyelination, damaged axon and proliferated astroglia. This effect was amplified by high-fat diet and postnatal hypoxia.
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China.
authors.forename: Lingxing
authors.initials: L
authors.lastname: Wang
authors.forename: Ruowei
authors.initials: R
authors.lastname: Cai
authors.forename: Guorong
authors.initials: G
authors.lastname: Lv
authors.forename: Ziyang
authors.initials: Z
authors.lastname: Huang
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology*; D001369:Axons / Q000378:metabolism / Q000473:pathology*; D002552:Cerebral Ventricles / Q000378:metabolism / Q000473:pathology*; D004041:Dietary Fats / Q000008:administration & dosage; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism / Q000473:pathology*; D018811:Maternal Exposure; D008431:Maternal-Fetal Exchange; D004676:Myelin Basic Protein / Q000378:metabolism; D016900:Neurofilament Proteins / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004041:Dietary Fats; D005904:Glial Fibrillary Acidic Protein; D004676:Myelin Basic Protein; D016900:Neurofilament Proteins; C044882:neurofilament protein L; C051774:neurofilament protein H