title: Protection against Helicobacter pylori infection by a trivalent fusion vaccine based on a fragment of urease B-UreB414.
abstract: A multivalent fusion vaccine is a promising option for protection against Helicobacter pylori infection. In this study, UreB414 was identified as an antigenic fragment of urease B subunit (UreB) and it induced an antibody inhibiting urease activity. Immunization with UreB414 partially protected mice from H. pylori infection. Furthermore, a trivalent fusion vaccine was constructed by genetically linking heat shock protein A (HspA), H. pylori adhesin A (HpaA), and UreB414, resulting in recombinant HspA-HpaA-UreB414 (rHHU). Its protective effect against H. pylori infection was tested in BALB/c mice. Oral administration of rHHU significantly protected mice from H. pylori infection, which was associated with H. pylori-specific antibody production and Th1/Th2-type immune responses. The results show that a trivalent fusion vaccine efficiently combats H. pylori infection, and that an antigenic fragment of the protein can be used instead of the whole protein to construct a multivalent vaccine.
authors.affiliation: Department of Pharmacy, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Liu
authors.forename: Shi
authors.initials: S
authors.lastname: Yun
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Yuan
authors.forename: Xu-Hu
authors.initials: XH
authors.lastname: Mao
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Kai-Yun
authors.initials: KY
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Dong-Shui
authors.initials: DS
authors.lastname: Lu
authors.forename: Wen-De
authors.initials: WD
authors.lastname: Tong
authors.forename: Ai-Dong
authors.initials: AD
authors.lastname: Wen
authors.forename: Quan-Ming
authors.initials: QM
authors.lastname: Zou
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology*; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005260:Female; D006360:Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D016481:Helicobacter Infections / Q000276:immunology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013270:Stomach / Q000382:microbiology / Q000473:pathology; D014510:Urease / Q000235:genetics / Q000276:immunology*; D037521:Virulence Factors / Q000235:genetics / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins; C113725:HspA protein, bacteria; D011993:Recombinant Fusion Proteins; D037521:Virulence Factors; C095977:adhesin A, Helicobacter; D015847:Interleukin-4; D007371:Interferon-gamma; D014510:Urease