title: Experimental investigation of pulsatility effect on the deformability and hemolysis of blood cells.
abstract: In this study, we investigated the differences between pulsatile cardiopulmonary bypass (CPB) procedure and nonpulsatile CPB procedure in terms of their effects on hemolysis and deformability of red blood cells (RBCs) under various shear stress conditions. In order to research the effects on hemolysis and deformability, four parameters--free hemoglobin (fHb) concentration, normalized index of hemolysis (NIH), deformability index (DI) of RBCs, and elongation index of RBCs--have been deeply investigated. For these investigations, two randomly assigned adult mongrel dog groups-nonpulsatile group (NP, n = 6) and pulsatile group (P, n = 6)--were examined. According to our results, both types of perfusion did not show any statistical differences in terms of the concentrations of fHb as well as NIH. In addition, there were no significant differences in RBC deformability between perfusion types within an operation time of 3 h. Therefore, our studies suggest that pulsatile perfusion has no significant difference from nonpulsatile perfusion in terms of hemolysis and deformability of RBCs.
authors.affiliation: Department of Mechatronics, Gwangju Institute of Science and Technology, Buk-gu, Gwangju, Korea.
authors.forename: Yang Jun
authors.initials: YJ
authors.lastname: Kang
authors.forename: Myoung Gon
authors.initials: MG
authors.lastname: Kim
authors.forename: Kuk Hui
authors.initials: KH
authors.lastname: Son
authors.forename: Choon Hak
authors.initials: CH
authors.lastname: Lim
authors.forename: Ho Sung
authors.initials: HS
authors.lastname: Son
authors.forename: Sang Youl
authors.initials: SY
authors.lastname: Yoon
authors.forename: Hyuk Sang
authors.initials: HS
authors.lastname: Kwon
authors.forename: Sung
authors.initials: S
authors.lastname: Yang
mesh_terms: D000818:Animals; D002315:Cardiopulmonary Bypass / Q000009:adverse effects* / Q000379:methods*; D004285:Dogs; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000166:cytology*; D006461:Hemolysis; D007091:Image Processing, Computer-Assisted; D011673:Pulsatile Flow; D011897:Random Allocation; D018709:Statistics, Nonparametric