Bacillus anthracis genome organization in light of whole transcriptome sequencing.
Emerging knowledge of whole prokaryotic transcriptomes could validate a number of theoretical concepts introduced in the early days of genomics. What are the rules connecting gene expression levels with sequence determinants such as quantitative scores of promoters and terminators? Are translation efficiency measures, e.g. codon adaptation index and RBS score related to gene expression? We used the whole transcriptome shotgun sequencing of a bacterial pathogen Bacillus anthracis to assess correlation of gene expression level with promoter, terminator and RBS scores, codon adaptation index, as well as with a new measure of gene translational efficiency, average translation speed. We compared computational predictions of operon topologies with the transcript borders inferred from RNA-Seq reads. Transcriptome mapping may also improve existing gene annotation. Upon assessment of accuracy of current annotation of protein-coding genes in the B. anthracis genome we have shown that the transcriptome data indicate existence of more than a hundred genes missing in the annotation though predicted by an ab initio gene finder. Interestingly, we observed that many pseudogenes possess not only a sequence with detectable coding potential but also promoters that maintain transcriptional activity.
Martin
Jeffrey
J
DOE Joint Genome Institute, Walnut Creek, CA 94598, USA.
Zhu
Wenhan
W
Passalacqua
Karla D
KD
Bergman
Nicholas
N
Borodovsky
Mark
M
D000465:Algorithms; D001408:Bacillus anthracis / Q000235:genetics*; D002874:Chromosome Mapping; D003062:Codon; D020869:Gene Expression Profiling / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008390:Markov Chains; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D012270:Ribosomes; D013728:Terminator Regions, Genetic
D003062:Codon; D012329:RNA, Bacterial