P450Arom induction in isolated control endometrial cells by peritoneal fluid from women with endometriosis.
OBJECTIVE
To study the effect of peritoneal fluid from women with (PF-E) and without (PF-C) endometriosis on P(450)Arom expression in endometrial cells.


METHODS
Experimental study.


METHODS
University research unit.


METHODS
Forty women of reproductive age with (n = 22) or without (control; n = 18) endometriosis.


METHODS
Peritoneal fluid and eutopic endometrial samples were obtained during surgery from women with (n = 13 and 9, respectively) and without (n = 4 and 14, respectively) endometriosis.


METHODS
Expression study for P(450)Arom, steroid factor 1 (SF-1), chicken ovalbumin upstream transcription factor I (COUP-TFI), and COUP-TFII messenger RNA (reverse transcriptase-polymerase chain reacion) and/or protein (immunoblot) in isolated endometrial epithelial cells transfected or not with expression vector containing SF-1, COUP-TFI, or COUP-TFII complementary DNAs.


RESULTS
Basal messenger RNA and/or protein expression of P(450)Arom and SF-1 were augmented in endometriosis, and that of COUP-TF was diminished. In control cells, (Bu)(2)cAMP and PF-E increased P(450)Arom and SF-1 expression (but not COUP-TF expression) in a dose-dependent way, an effect not observed with PF-C, adsorbed PF-E, or 10(-5) M indomethacin. Transfected cells confirmed these results. Any treatments modified the studied molecules in endometriosis cells.


CONCLUSIONS
These data indicate that molecules contained in PF-E favor an estrogenic microenvironment, suggesting a role in the etiopathogenesis of endometriosis enabling the survival, maintenance, and growth of endometrial implants in the ectopic locations.
Castro
Jazmin
J
Institute of Maternal and Child Research, Faculty of Medicine, University of Chile, Santiago, Chile.
Torres
Marisa
M
Sovino
Hugo
H
Fuentes
Ariel
A
Boric
M Angélica
MA
Johnson
M Cecilia
MC
D000328:Adult; D001141:Aromatase / Q000096:biosynthesis* / Q000235:genetics; D001202:Ascitic Fluid / Q000473:pathology* / Q000502:physiology*; D051836:COUP Transcription Factors / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D002469:Cell Separation; D002478:Cells, Cultured; D004715:Endometriosis / Q000378:metabolism / Q000473:pathology*; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D004790:Enzyme Induction; D005260:Female; D006801:Humans; D008875:Middle Aged; D010532:Peritoneal Diseases / Q000378:metabolism / Q000473:pathology*; D054339:Steroidogenic Factor 1 / Q000235:genetics / Q000378:metabolism
D051836:COUP Transcription Factors; C515057:NR5A1 protein, human; D054339:Steroidogenic Factor 1; D001141:Aromatase