title: Complement as an endogenous adjuvant for dendritic cell-mediated induction of retrovirus-specific CTLs.
abstract: Previous studies have demonstrated the involvement of complement (C) in induction of efficient CTL responses against different viral infections, but the exact role of complement in this process has not been determined. We now show that C opsonization of retroviral particles enhances the ability of dendritic cells (DCs) to induce CTL responses both in vitro and in vivo. DCs exposed to C-opsonized HIV in vitro were able to stimulate CTLs to elicit antiviral activity significantly better than non-opsonized HIV. Furthermore, experiments using the Friend virus (FV) mouse model illustrated that the enhancing role of complement on DC-mediated CTL induction also occurred in vivo. Our results indicate that complement serves as natural adjuvant for DC-induced expansion and differentiation of specific CTLs against retroviruses.
authors.affiliation: Department of Hygiene, Microbiology and Social Medicine, Division of Virology, Innsbruck Medical University, Innsbruck, Tirol, Austria.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Bánki
authors.forename: Wilfried
authors.initials: W
authors.lastname: Posch
authors.forename: Asim
authors.initials: A
authors.lastname: Ejaz
authors.forename: Verena
authors.initials: V
authors.lastname: Oberhauser
authors.forename: Suzanne
authors.initials: S
authors.lastname: Willey
authors.forename: Christoph
authors.initials: C
authors.lastname: Gassner
authors.forename: Heribert
authors.initials: H
authors.lastname: Stoiber
authors.forename: Ulf
authors.initials: U
authors.lastname: Dittmer
authors.forename: Manfred P
authors.initials: MP
authors.lastname: Dierich
authors.forename: Kim J
authors.initials: KJ
authors.lastname: Hasenkrug
authors.forename: Doris
authors.initials: D
authors.lastname: Wilflingseder
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006678:HIV / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D012192:Retroviridae Infections / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D003165:Complement System Proteins