title: Surface functionalisation of PLGA nanoparticles for gene silencing.
abstract: This work presents a method for decorating the surface of poly (lactide-co-glycolide) (PLGA) nanoparticles with polyethyleneimine (PEI) utilising a cetyl derivative to improve surface functionalisation and siRNA delivery. Sub-micron particles were produced by an emulsion-diffusion method using benzyl alcohol. We demonstrate by x-ray photoelectron spectroscopy (XPS), 2.6 times higher surface presentation of amines using the cetyl derivative compared to non-cetylated-PEI formulations (6.5 and 2.5% surface nitrogen, respectively). The modified particles were shown by spectroscopy, fluorescent microscopy and flow cytometry to bind and mediate siRNA delivery into the human osteosarcoma cell line U2OS and the murine macrophage cell line J774.1. Specific reduction in the anti-apoptotic oncogene BCL-w in U2OS cells was achieved with particles containing cetylated-PEI (53%) with no cellular toxicity. In addition, particles containing cetylated-PEI achieved 64% silencing of TNFalpha in J774.1 cells. This rapid method for surface modification of PLGA nanoparticles promotes its application for alternative cetylated functional derivatives as a strategy to control specific biological properties of nanoparticles.
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Ny Munkegade 118, University of Aarhus, Aarhus C, Denmark.
authors.forename: Morten Ø
authors.initials: MØ
authors.lastname: Andersen
authors.forename: Agata
authors.initials: A
authors.lastname: Lichawska
authors.forename: Ayyoob
authors.initials: A
authors.lastname: Arpanaei
authors.forename: Stig Møller
authors.initials: SM
authors.lastname: Rask Jensen
authors.forename: Harpreet
authors.initials: H
authors.lastname: Kaur
authors.forename: David
authors.initials: D
authors.lastname: Oupicky
authors.forename: Flemming
authors.initials: F
authors.lastname: Besenbacher
authors.forename: Peter
authors.initials: P
authors.lastname: Kingshott
authors.forename: Jørgen
authors.initials: J
authors.lastname: Kjems
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Howard
mesh_terms: D000818:Animals; D002460:Cell Line; D020868:Gene Silencing; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D034741:RNA, Small Interfering / Q000378:metabolism; D013499:Surface Properties
chemical_list: D034741:RNA, Small Interfering; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011094:Polyethyleneimine