Study on molecular structural characteristics of tea polysaccharide.
Tea polysaccharide (TPS) is attracting more attention gradually due to its particular biological properties. However, molecular characteristics of TPS are unclear since appropriate method is still absent. So, study on the molecular characteristics of TPS was carried out by high-performance size-exclusion chromatography (HPSEC), multi-angle laser light scattering (MALLS) and viscosimetry. The results showed that the molar masses of TPS ranged from 2.287 x 10(5) to 2.762 x 10(5)gmol(-1), the RMS radii distributed from 132.1 to 145.9 nm, and M(w)/M(n) is 1.028. The Mark-Houwink equation was established as [eta]=0.5423 M(w)(0.5379), and the intrinsic viscosity and molecular chain parameters were as follows: [eta]=1.007 dL g(-1), k(H)=0.845, k(K)=0.387, alpha=0.5379, K=0.5423. In addition, based on the slope of the RMS radius versus molar mass conformational plot being 0.24+/-0.00, we suggest that the molecular morphology of TPS is a homogeneous and spherical polymer with branch in solution.
Guo
Li
L
Key Laboratory of Tea Biochemistry & Biotechnology, Anhui Agricultural University, 130 Western Changjiang Road, Hefei City, Anhui Province 230036, China.
Du
Xianfeng
X
Lan
Jing
J
Liang
Qin
Q
D000818:Animals; D028241:Camellia sinensis / Q000737:chemistry; D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D007834:Lasers; D008027:Light; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry*; D012542:Scattering, Radiation; D013057:Spectrum Analysis; D013662:Tea / Q000737:chemistry*; D014783:Viscosity
D011134:Polysaccharides; D013662:Tea