Chemokine ligand 2 genetic variants, serum monocyte chemoattractant protein-1 levels, and the risk of coronary artery disease.
OBJECTIVE
In humans, evidence about the association between levels of monocyte chemoattractant protein-1 (MCP-1), its coding gene chemokine (C-C motif) ligand 2 (CCL2), and risk of coronary artery disease (CAD) is contradictory.


RESULTS
We performed a nested case-control study in the prospective EPIC-Norfolk cohort investigating the relationship between CCL2 single-nucleotide polymorphisms (SNPs), MCP-1 concentrations, and the risk of future CAD. Cases (n=1138) were apparently healthy men and women aged 45 to 79 years who developed fatal or nonfatal CAD during a mean follow-up of 6 years. Controls (n=2237) were matched by age, sex, and enrollment time. Using linear regression analysis no association between CCL2 SNPs and MCP-1 serum concentrations became apparent, nor did we find a significant association between MCP-1 serum levels and risk of future CAD. Finally, Cox regression analysis showed no significant association between CCL2 SNPs and the future CAD risk. In addition, we did not find any robust associations between the CCL2 haplotypes and MCP-1 serum concentration or future CAD risk.


CONCLUSIONS
Our data do not support previous publications indicating that MCP-1 is involved in the pathogenesis of CAD.
van Wijk
Diederik F
DF
Department of Vascular Medicine, Academic Medical Center, Amsterdam, the Netherlands. d.f.vanwijk@amc.uva.nl
van Leuven
Sander I
SI
Sandhu
Manjinder S
MS
Tanck
Michael W
MW
Hutten
Barbara A
BA
Wareham
Nicholas J
NJ
Kastelein
John J P
JJ
Stroes
Erik S G
ES
Khaw
Kay-Tee
KT
Boekholdt
S Matthijs
SM
D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000097:blood / Q000235:genetics*; D003324:Coronary Artery Disease / Q000235:genetics* / Q000276:immunology; D004739:England; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D016016:Proportional Hazards Models; D011446:Prospective Studies; D018570:Risk Assessment; D012307:Risk Factors; D013997:Time Factors
D015415:Biomarkers; C111835:CCL2 protein, human; D018932:Chemokine CCL2