title: High prevalence, low counts and uncommon serotypes of Listeria monocytogenes in linguiça, a Brazilian fresh pork sausage.
abstract: Linguiça is a highly popular and appreciated pork product in Brazil, frequently consumed undercooked. Aiming at collection of data for a future risk assessment, this study evaluated the prevalence and counts of Listeria monocytogenes in linguiça samples collected at retail level in Sao Paulo, SP, Brazil. ISO methods were used for detection and enumeration of the pathogen (11290-1 and 11290-2, respectively). Isolates were submitted to Simplex-PCR for hlyA gene and those with biochemical features of L. monocytogenes and hlyA positive were serotyped using a Multiplex PCR. Ninety percent of the samples were positive for Listeria spp., and L. monocytogenes was detected in 42% of the samples, with counts below 10(2)CFU/g in all samples. A prevalence of uncommon serotypes 4a and 4c was observed.
authors.affiliation: University of Sao Paulo, Faculty of Pharmaceutical Sciences, Department of Food and Experimental Nutrition, Av. Prof. Lineu Prestes, 580, 05508-900 Sao Paulo, SP, Brazil.
authors.forename: Keila Naomi
authors.initials: KN
authors.lastname: Miyasaki
authors.forename: Eb
authors.initials: E
authors.lastname: Chiarini
authors.forename: Anderson de Souza
authors.initials: Ade S
authors.lastname: Sant Ana
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Destro
authors.forename: Mariza
authors.initials: M
authors.lastname: Landgraf
authors.forename: Bernadette Dora Gombossy de Melo
authors.initials: BD
authors.lastname: Franco