Targeted gene deletions in C. elegans using transposon excision.
We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.
Frøkjaer-Jensen
Christian
C
Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City, Utah, USA.
Davis
M Wayne
MW
Hollopeter
Gunther
G
Taylor
Jon
J
Harris
Todd W
TW
Nix
Paola
P
Lofgren
Rachel
R
Prestgard-Duke
Michael
M
Bastiani
Michael
M
Moerman
Donald G
DG
Jorgensen
Erik M
EM
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D004260:DNA Repair; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000502:physiology; D017353:Gene Deletion; D019895:Transposases / Q000502:physiology
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C111652:mariner transposases; D019895:Transposases