title: Expression of thermostable bacterial beta-glucosidase (BglB) in transgenic tobacco plants.
abstract: Transgenic tobacco plants expressing the hyperthermostable beta-glucosidase BglB of Thermotoga maritima were generated with the goal of cost-effective production of the enzyme for the application in bioconversion of lignocellulosic biomass. The enzyme was targeted to the cytosol and chloroplasts, where it accumulated to level of 4.5% and 5.8% of total soluble protein, respectively. The optimal temperature and pH of the plant-expressed BglB was 80 degrees C and 4.5, respectively. BglB activity was preserved in leaves after lyophilization, but decreased by over 70% with drying at room temperature. When BglB was synergistically supplied in a 1% (w/v) rice straw with Cel5A for efficient cellulase conversion, a 37% increase in glucose was observed. This report demonstrates the potential of utilizing transgenic tobacco for mass production of BglB.
authors.affiliation: Department of Forest Products and Technology (BK21 Program), Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Sera
authors.initials: S
authors.lastname: Jung
authors.forename: Suyeon
authors.initials: S
authors.lastname: Kim
authors.forename: Hanhong
authors.initials: H
authors.lastname: Bae
authors.forename: Hyoun-Sub
authors.initials: HS
authors.lastname: Lim
authors.forename: Hyeun-Jong
authors.initials: HJ
authors.lastname: Bae
mesh_terms: D004789:Enzyme Activation; D004795:Enzyme Stability; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism*; D013696:Temperature; D020124:Thermotoga maritima / Q000201:enzymology*; D001617:beta-Glucosidase / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D001617:beta-Glucosidase