Efficient solid-phase chemical synthesis of 5'-triphosphates of DNA, RNA, and their analogues.
A robust, reproducible, and scalable method for the solid-phase synthesis of 5'-triphosphates of DNA, RNA, and their chemically modified analogues using 5'-H-phosphonate intermediates is described. 5'-Triphosphates of oligonucleotides with varying lengths and sequences containing different 5'-terminal nucleotides, with and without internal sugar-backbone modifications, were efficiently prepared as crude products or further purified by HPLC.
Zlatev
Ivan
I
Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Université Montpellier 1 - Université Montpellier 2, Place Eugene Bataillon, CC1704, 34095 Montpellier Cedex 5, France.
Lavergne
Thomas
T
Debart
Françoise
F
Vasseur
Jean-Jacques
JJ
Manoharan
Muthiah
M
Morvan
François
F
D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D063065:Organophosphonates / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D052616:Solid Phase Extraction
D063065:Organophosphonates; D012313:RNA; D004247:DNA