title: Proteomic analysis of epicardial and subcutaneous adipose tissue reveals differences in proteins involved in oxidative stress.
abstract: Epicardial adipose tissue (EAT) is an endocrine organ adjacent to coronary arteries and myocardium without anatomy barriers. Locally produced adipokines may reflect or affect to cardiovascular physiology and pathology. Our aim was to study the protein expression profiles of EAT and subcutaneous adipose tissue (SAT) to identify local candidate molecules characterizing EAT in patients with cardiovascular disease. EAT and SAT samples were collected from 55 patients undergoing heart surgery. Proteins from these tissues were separated by two-dimensional (2D) gel electrophoresis, and differences between them were identified by MALDI-TOF/TOF spectra. Differences in protein levels were further investigated by real-time RT-PCR and Western blots, and production of reactive oxygen species (ROS) in EAT and SAT was evaluated by nitroblue tetrazolium chloride assays. ROS production was higher in EAT than SAT. We have found mRNA differences for catalase, glutathione S-transferase P, and protein disulfide isomerase, and 2D Western blots additionally showed post-translational differences for phosphoglycerate mutase 1; all four are related to oxidative stress pathways. EAT suffers greater oxidative stress than SAT in patients with cardiovascular diseases and exhibits associated proteomic differences that suggest the possibility of its association with myocardial stress in these patients.
authors.affiliation: Laboratory 6, Area Cardiovascular, Instituto de Investigaciones Sanitarias, Complejo Hospitalario Universitario de Santiago, Santiago de Compostela, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Salgado-Somoza
authors.forename: Elvis
authors.initials: E
authors.lastname: Teijeira-Fernández
authors.forename: Angel Luis
authors.initials: AL
authors.lastname: Fernández
authors.forename: José Ramón
authors.initials: JR
authors.lastname: González-Juanatey
authors.forename: Sonia
authors.initials: S
authors.lastname: Eiras
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002318:Cardiovascular Diseases / Q000235:genetics / Q000378:metabolism* / Q000601:surgery; D002374:Catalase / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005982:Glutathione Transferase / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008875:Middle Aged; D018384:Oxidative Stress; D010496:Pericardium / Q000737:chemistry*; D010736:Phosphoglycerate Mutase / Q000032:analysis; D019704:Protein Disulfide-Isomerases / Q000032:analysis; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D017382:Reactive Oxygen Species / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D050151:Subcutaneous Fat / Q000737:chemistry*
chemical_list: D011506:Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D002374:Catalase; D005982:Glutathione Transferase; D019704:Protein Disulfide-Isomerases; D010736:Phosphoglycerate Mutase