title: Immunoglobulin profiles in acute Brucellosis experimentally induced by Brucella canis in BALB/c mice.
abstract: This study evaluated profiles of immunoglobulin (Ig; IgA, IgG, IgG1, and IgG2a) response in experimental brucellosis induced with Brucella canis in BALB/c mice during an 8-week infection period. Six- to 8-week-old BALB/c mice (n = 36) were experimentally infected with 1 × 10(9) CFU of B. canis via the intraperitoneal route. Serial serum samples were collected from the mice at 0, 3, 7, 14, 21, 28, 35, 42, 49, and 56 days after inoculation. The sera were tested by the rapid slide agglutination test (RSAT) and 2-mercaptoethanol-RSAT and indirect enzyme-linked immunosorbent assay. Sera tested positive for B. canis by the RSAT and 2-mercaptoethanol-RSAT beginning from 7 days after inoculation until the end of the experiment. The IgA response was detected at 14 days after infection and reached peak levels at 21 days after infection. The IgG antibody responses were detected at 7 days after infection and reached the peak value at 35 days after infection. Data of our study demonstrated IgG2a-dominant responses over IgG1 during the course of infection (p > 0.05).
authors.affiliation: Division of Model Animal, Institute of Biomedical Science, Kansai Medical University, Osaka, Japan.
authors.forename: Sung-Il
authors.initials: SI
authors.lastname: Lee
authors.forename: Md Ariful
authors.initials: MA
authors.lastname: Islam
authors.forename: Mst Minara
authors.initials: MM
authors.lastname: Khatun
authors.forename: Gyu-Young
authors.initials: GY
authors.lastname: Choi
authors.forename: Jae-Myeong
authors.initials: JM
authors.lastname: Jung
authors.forename: Byeong-Kirl
authors.initials: BK
authors.lastname: Baek
authors.forename: Ibulaimu
authors.initials: I
authors.lastname: Kakoma
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D041002:Brucella canis / Q000276:immunology; D002006:Brucellosis / Q000097:blood*; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007136:Immunoglobulins / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007136:Immunoglobulins