title: Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and proteins.
abstract: We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomaterials for small analytes (e.g., glutathione); Pt nanosponges and Fe(3)O(4) NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomaterials have several advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations (fewer problems associated with "sweet spots"), when used in laser desorption/ionization mass spectrometry.
authors.affiliation: Department of Chemistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Cheng-Kang
authors.initials: CK
authors.lastname: Chiang
authors.forename: Ni-Chen
authors.initials: NC
authors.lastname: Chiang
authors.forename: Zong-Hong
authors.initials: ZH
authors.lastname: Lin
authors.forename: Guo-Yu
authors.initials: GY
authors.lastname: Lan
authors.forename: Yang-Wei
authors.initials: YW
authors.lastname: Lin
authors.forename: Huan-Tsung
authors.initials: HT
authors.lastname: Chang
mesh_terms: D045304:Cytochromes c / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D016014:Linear Models; D053768:Metal Nanoparticles / Q000737:chemistry; D008970:Molecular Weight; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D010455:Peptides; D011506:Proteins; D010984:Platinum; D006046:Gold; D045304:Cytochromes c; D052203:Ferrosoferric Oxide