title: In vitro cytotoxic activity of isolated acridones alkaloids from Zanthoxylum leprieurii Guill. et Perr.
abstract: Chemical investigation of the roots and fruits of Zanthoxylumleprieurii Guill. et Perr. led to the isolation of three new alkaloids including two acridone derivatives, 3-hydroxy-1,4-dimethoxy-10-methyl-9-acridone (2) and 3-hydroxy-1,2-dimethoxy-10-methyl-9-acridone (3) named helebelicine A and B, respectively, and one secobenzo[c]phenantridine, 10-O-demethyl-12-O-methylarnottianamide (10), together with thirteen other compounds. The structures of compounds 2, 3 and 10 as well as those of the known compounds were elucidated by using spectroscopic methods and by comparison with reported data. The brine-shrimp (artemia salina) lethality bioassay of the chloroform extract of the fruits showed modest cytotoxicity with LD(50) at 13.1microg/mL. Isolated compounds 1, 4-6 were found to be moderately active against lung carcinoma cells (A549), colorectal adenocarcinoma cells (DLD-1) and normal cells (WS1) with IC(50) values ranging from 27 to 77microM. In contrast to the positive control etoposide used, the cytotoxicity of the most active compound 4 was found to be selective against cancer cells in comparison to normal cells WS1 with IC(50) of 51+/-8microM and 4.3+/-0.4microM, respectively.
authors.affiliation: Department of Organic Chemistry, Faculty of Science, University of Yaoundé I, PO Box 812, Yaoundé, Cameroon. manfouo@yahoo.fr
authors.forename: Rostand M
authors.initials: RM
authors.lastname: Ngoumfo
authors.forename: Jean-Bosco
authors.initials: JB
authors.lastname: Jouda
authors.forename: Ferdinand T
authors.initials: FT
authors.lastname: Mouafo
authors.forename: Justin
authors.initials: J
authors.lastname: Komguem
authors.forename: Céline D
authors.initials: CD
authors.lastname: Mbazoa
authors.forename: Tze Chieh
authors.initials: TC
authors.lastname: Shiao
authors.forename: Mohammed I
authors.initials: MI
authors.lastname: Choudhary
authors.forename: Hartmut
authors.initials: H
authors.lastname: Laatsch
authors.forename: Jean
authors.initials: J
authors.lastname: Legault
authors.forename: André
authors.initials: A
authors.lastname: Pichette
authors.forename: René
authors.initials: R
authors.lastname: Roy
mesh_terms: D054831:Acridones / Q000494:pharmacology*; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001156:Artemia / Q000187:drug effects; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: D054831:Acridones; D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts