International multicenter study to assess a panel of reference materials for quantification of simian immunodeficiency virus RNA in plasma.
An international multicenter study was conducted to assess the performance of a panel of simian immunodeficiency virus (SIV) RNA reference materials for plasma viral load determinations. Reliable quantification was demonstrated across an approximately 6 log(10) dynamic range. Availability of external reference materials will enable independent calibration of SIV plasma viral load assays.
Ham
Claire
C
Division of Retrovirology, NIBSC/HPA, South Mimms, Potters Bar, Hertfordshire, United Kingdom.
Srinivasan
Priya
P
Thorstensson
Rigmor
R
Verschoor
Ernst
E
Fagrouche
Zahra
Z
Sernicola
Leonardo
L
Ramos
Artur
A
Titti
Fausto
F
Almond
Neil
N
Berry
Neil
N
D000818:Animals; D016014:Linear Models; D008252:Macaca fascicularis / Q000821:virology; D012367:RNA, Viral / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012680:Sensitivity and Specificity; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000302:isolation & purification
D012367:RNA, Viral