title: Continued persistence of a single genotype of dengue virus type-3 (DENV-3) in Delhi, India since its re-emergence over the last decade.
abstract: OBJECTIVE
  The re-emergence of an epidemic strain of dengue virus type-3 (DENV-3) in Delhi in 2003 and its persistence in subsequent years marked a changing trend in dengue virus circulation in this part of India. Its evolving phylogeny over the past decade has not been studied in detail as yet.
  
  
  METHODS
  Reverse transcription polymerase chain reaction and sequencing of the CprM gene junction of DENV-3 from different outbreaks since 2003 was carried out. Thirty CprM DENV-3 sequences from this study were compared with 46 other previously reported CprM DENV-3 sequences from India and other countries. Multiple sequence alignment and phylogenetic trees were constructed to determine the extent of genetic heterogeneity and trace the phylogeny of DENV-3.
  
  
  RESULTS
  Thirty CprM DENV-3 sequences (Accession numbers AY706096-99, DQ645945-52, EU181201-14, and EU846234-36) were submitted to GenBank. The CprM junction was found to be AT rich (approximately 53%). Nucleotide sequence alignment revealed only nucleotide substitutions. Phylogenetic analysis indicated sustained evolution of a distinct Indian lineage of DENV-3 genotype III in Delhi.
  
  
  CONCLUSIONS
  Active circulation of DENV-3 genotype III over the last decade in Delhi was evident and worrying. This genotype has been implicated in several outbreaks in South-East Asia and other parts of the world.
authors.affiliation: Division of Biochemistry and Biotechnology, National Institute of Communicable Diseases, Delhi, India.
authors.forename: Himani
authors.initials: H
authors.lastname: Kukreti
authors.forename: Veena
authors.initials: V
authors.lastname: Mittal
authors.forename: Artee
authors.initials: A
authors.lastname: Chaudhary
authors.forename: Rajendra Singh
authors.initials: RS
authors.lastname: Rautela
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.forename: Sheetal
authors.initials: S
authors.lastname: Chauhan
authors.forename: Sunil
authors.initials: S
authors.lastname: Bhat
authors.forename: Mala
authors.initials: M
authors.lastname: Chhabra
authors.forename: Dipesh
authors.initials: D
authors.lastname: Bhattacharya
authors.forename: Syed Tazeen
authors.initials: ST
authors.lastname: Pasha
authors.forename: Devinder
authors.initials: D
authors.lastname: Kumar
authors.forename: Sunil
authors.initials: S
authors.lastname: Gomber
authors.forename: Shiv
authors.initials: S
authors.lastname: Lal
authors.forename: Arvind
authors.initials: A
authors.lastname: Rai
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D003715:Dengue / Q000453:epidemiology* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D005260:Female; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D055815:Young Adult
chemical_list: D012367:RNA, Viral