Evaluation of hepcidin isoforms in hemodialysis patients by a proteomic approach based on SELDI-TOF MS.
The hepatic iron regulator hormone hepcidin consists, in its mature form, of 25 amino acids, but two other isoforms, hepcidin-20 and hepcidin-22, have been reported, whose biological meaning remains poorly understood. We evaluated hepcidin isoforms in sera from 57 control and 54 chronic haemodialysis patients using a quantitative proteomic approach based on SELDI-TOF-MS. Patients had elevated serum levels of both hepcidin-25 and hepcidin-20 as compared to controls (geometric means: 7.52 versus 4.69 nM, and 4.06 versus 1.76 nM, resp., P < .05 for both). The clearance effects of a single dialysis session by different dialysis techniques and membranes were also investigated, showing an average reduction by 51.3% +/- 29.2% for hepcidin-25 and 34.2% +/- 28.4% for hepcidin-20 but only minor differences among the different dialysis modalities. Measurement of hepcidin isoforms through MS-based techniques can be a useful tool for better understanding of their biological role in hemodialysis patients and other clinical conditions.
Campostrini
Natascia
N
Department of Medicine, University of Verona, 37134 Verona, Italy.
Castagna
Annalisa
A
Zaninotto
Federica
F
Bedogna
Valeria
V
Tessitore
Nicola
N
Poli
Albino
A
Martinelli
Nicola
N
Lupo
Antonio
A
Olivieri
Oliviero
O
Girelli
Domenico
D
D000328:Adult; D000368:Aged; D023181:Antimicrobial Cationic Peptides / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D064451:Hepcidins; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood*; D020033:Protein Isoforms; D040901:Proteomics / Q000379:methods; D006435:Renal Dialysis / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D023181:Antimicrobial Cationic Peptides; D064451:Hepcidins; D010446:Peptide Fragments; D020033:Protein Isoforms; C542573:hepcidin (6-25), human; C545475:hepcidin 25, human