Development of a convenient immunochromatographic strip for the diagnosis of infection with Japanese encephalitis virus in swine.
Japanese encephalitis (JE) is caused by the Japanese encephalitis virus (JEV). It is a major public health problem in Asia. JEV infects swine which results in fatal encephalitis, abortion and stillbirth in pregnant sow, and hypospermia in boars. Swine is a viral amplifier, and thus plays a critical role in JEV transmission. Thus, development of a rapid method for JEV detection in swine is required for clinical JE diagnosis, as well as to suppress viral spread. In this study, a convenient and rapid immunochromatographic strip (ICS) was developed for detecting JEV in swine using two monoclonal antibodies (MAbs) (2A2 and 4D1) against the E protein of JEV. Results showed that colloidal gold-conjugated MAbs 2A2 (CG-MAb) bond with JEV and the resulting complex was held by the other MAb 4D1 at the test line to give a reddish-purple band. Sensitivity tests demonstrated that ICS can detect 2.5x10(5)PFU of JEV. The clinical screening results showed that the specificity and sensitivity of the ICS were 99.3% and 85.7% respectively as compared to that of RT-PCR. This suggests that the MAbs-based ICS test can be used as a convenient method for the rapid detection of JEV in infected swine samples.
Li
Yaoming
Y
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Hou
Lidan
L
Ye
Jing
J
Liu
Xueqin
X
Dan
Hanbing
H
Jin
Meilin
M
Chen
Huanchun
H
Cao
Shengbo
S
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D004664:Encephalitis Virus, Japanese / Q000276:immunology / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017979:Gold Colloid; D007118:Immunoassay / Q000379:methods; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology*; D014773:Virology / Q000379:methods*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D017979:Gold Colloid