title: TP53 mutations in Korean patients with non-small cell lung cancer.
abstract: Although TP53 mutations have been widely studied in lung cancer, the majority of studies have focused on exons 5-8 of the gene. In addition, TP53 mutations in Korean patients with lung cancers have not been investigated. We searched for mutations in the entire coding exons, including splice sites of the gene, in Korean patients with non-small cell lung cancer (NSCLC). Mutations of the gene were determined by direct sequencing in 176 NSCLCs. Sixty-nine mutations (62 different mutations) were identified in 65 tumors. Of the 62 mutations, 12 were novel mutations. TP53 mutations were more frequent in males, ever-smokers and squamous cell carcinomas than in females, never-smokers and adenocarcinomas, respectively (all comparisons, P<0.001). Missense mutations were most common (52.2%), but frameshift, nonsense, and splice-site mutations were frequently observed at frequencies of 18.8%, 15.9% and 10.1%, respectively. Of the 69 mutations, 9 (13.0%) were found in the oligomerization domain. In addition, the proportion of mutations in the oligomerization domain was significantly higher in adenocarcinomas than in squamous cell carcinomas (23.5% vs. 2.9%, P=0.01). Our study provides clinical and molecular characteristics of TP53 mutations in Korean patients with NSCLCs.
authors.affiliation: Department of Thoracic Surgery, School of Medicine, Kyungpook National University, Daegu, Korea.
authors.forename: Eung Bae
authors.initials: EB
authors.lastname: Lee
authors.forename: Guang
authors.initials: G
authors.lastname: Jin
authors.forename: Shin Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Park
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Jin Eun
authors.initials: JE
authors.lastname: Choi
authors.forename: Hyo Sung
authors.initials: HS
authors.lastname: Jeon
authors.forename: Seung Ick
authors.initials: SI
authors.lastname: Cha
authors.forename: Sukki
authors.initials: S
authors.lastname: Cho
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Tae-In
authors.initials: TI
authors.lastname: Park
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Jung
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Son
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Park
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000453:epidemiology* / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000235:genetics*; D006801:Humans; D015994:Incidence; D007723:Korea / Q000453:epidemiology; D008175:Lung Neoplasms / Q000453:epidemiology* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018570:Risk Assessment / Q000379:methods; D012307:Risk Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53