OprF/I-vaccinated sera inhibit binding of human interferon-gamma to Pseudomonas aeruginosa.
The recombinant outer membrane protein OprF/I has been demonstrated in previous studies to protect against Pseudomonas aeruginosa infection through a mechanism of enhanced antibody-mediated opsonophagocytosis. Recent evidence indicates that P. aeruginosa enhances its virulence phenotype as a consequence of binding to human IFN-gamma through an outer membrane protein, OprF. In this study, we demonstrate that a single boost injection of OprF/I vaccine elicited a strong OprF/I-specific antibody response in individuals who were previously vaccinated with OprF/I in a clinical trial. The OprF/I-vaccinated sera inhibit P. aeruginosa binding to IFN-gamma, suggesting an alternative mechanism by which the OprF/I vaccine confers protection against P. aeruginosa infection.
Ding
Bin
B
Centre for Clinical Research, University of Freiburg, 79106 Freiburg, Germany.
von Specht
Bernd-Ulrich
BU
Li
Yuanyi
Y
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology*; D011485:Protein Binding; D011552:Pseudomonas Infections / Q000276:immunology / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007106:Immune Sera; C547605:OprF protein, Pseudomonas aeruginosa; D011994:Recombinant Proteins; D007371:Interferon-gamma