Diagnosis and phylogenetic analysis of Orf virus from goats in China: a case report.
BACKGROUND
Orf virus (ORFV) is the etiological agent of contagious pustular dermatitis and is the prototype of the genus Parapoxvirus (PPV). It causes a severe exanthematous dermatitis that afflicts domestic and wild small ruminants.


METHODS
In the present study, an outbreak of proliferative dermatitis in farmed goats. The presence of ORFV in tissue scrapings from the lips was confirmed by B2L gene polymerase chain reaction (PCR) amplification. The molecular characterization of the ORFV was performed using PCR amplification, DNA sequencing and phylogenetic analysis of the B2L gene.


CONCLUSIONS
The results of this investigation indicated that the outbreak was caused by infection with an ORFV that was closely related genetically to Nantou (DQ934351), which was isolated from the Tai wan province of China and Hoping (EU935106), which originated from South Korea in 2008. This is the first report of the phylogenetic analysis of ORFV from goats in China.
Zhang
Keshan
K
Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping No,1, Yanchangpu, Lanzhou, Gansu, 730046, China.
Shang
Youjun
Y
Jin
Ye
Y
Wang
Guangxiang
G
Zheng
Haixue
H
He
Jijun
J
Lu
Zhongxin
Z
Liu
Xiangtao
X
D000818:Animals; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D004474:Ecthyma, Contagious / Q000821:virology*; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D008046:Lip / Q000821:virology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009923:Orf virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D004279:DNA, Viral