Simultaneous determination of baicalin, baicalein, wogonin, berberine, palmatine and jatrorrhizine in rat plasma by liquid chromatography-tandem mass spectrometry and application in pharmacokinetic studies after oral administration of traditional Chinese medicinal preparations containing scutellaria-coptis herb couple.
A sensitive, rapid and selective liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed and validated for the simultaneous determination of baicalin, baicalein, wogonin, berberine, palmatine and jatrorrhizine in scutellaria-coptis herb couple in rat plasma. After protein precipitation with acetonitrile and 0.1% NaH(2)PO(4), chromatography was performed using a C(18) column, with gradient elution with 0.1% formic acid and acetonitrile at 0.25 ml/min. All analytes including internal standards were monitored under positive ionization conditions by selected reaction monitoring with an electrospray ionization source. The lower limit of quantification was 10 ng/ml for baicalin, baicalein and wogonin, and 0.6 ng/ml for berberine, palmatine and jatrorrhizine. The validated method was applied in pharmacokinetic studies after oral administration of Yiqing Capsule and Gegen-Qinlian Tablet to rats.
Feng
Jin
J
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, No 415 Fengyang Road, Shanghai 200003, PR China.
Xu
Wen
W
Tao
Xia
X
Wei
Hua
H
Cai
Fei
F
Jiang
Bo
B
Chen
Wansheng
W
D000284:Administration, Oral; D000818:Animals; D001599:Berberine / Q000031:analogs & derivatives / Q000097:blood; D001600:Berberine Alkaloids / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods*; D039323:Coptis; D044950:Flavanones / Q000097:blood; D005419:Flavonoids / Q000097:blood; D008297:Male; D008516:Medicine, Chinese Traditional; D051381:Rats; D017207:Rats, Sprague-Dawley; D031363:Scutellaria; D053719:Tandem Mass Spectrometry / Q000379:methods*
D001600:Berberine Alkaloids; D044950:Flavanones; D005419:Flavonoids; C055785:jatrorrhizine; D001599:Berberine; C038044:baicalin; C006680:baicalein; C005413:palmatine; C085514:wogonin