[Preparation and authentication of beta-cyclodextrin complex of volatile oil from Ledum palustre].
OBJECTIVE
To study the optimum inclusion process conditions for the beta-cyclodextrin complex of volatile oil from Ledum palustre.


METHODS
The utilization ratio of the volatile oil from Ledum palustre was set as the index, and four factors were selected including the ratio of the volatile oil to beta-cyclodextrin, the temperature of preparation, the inclusion time and the ratio of beta-cyclodextrin to water. The optimum conditions were investigated by the orthogonal test form L9 (3(4)). The inclusion compound was identified by the ways of Micro-imaging, Thin-layer chromatography (TLC), Ultraviolet Spectroscopy (UV)and Infrared Spectroscopy (IR).


RESULTS
The optimum preparation conditions were as follows: volatile oils: beta-cyclodextrin was 1:8, the inclusion temperature and time was 50 degrees C and 30 min, respectively. Beta-cyclodextrin: water was 1:15.


CONCLUSIONS
The inclusion technology is stable and its quality is adjustable.
Xue
Fei
F
College of Pharmacy, Yanbian University, Yanji 133000, China.
Yin
Shou-yu
SY
D000704:Analysis of Variance; D002855:Chromatography, Thin Layer; D004337:Drug Carriers; D004355:Drug Stability; D029791:Ericaceae / Q000737:chemistry*; D009822:Oils, Volatile / Q000008:administration & dosage* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D047392:beta-Cyclodextrins
D004337:Drug Carriers; D009822:Oils, Volatile; D047392:beta-Cyclodextrins