title: Local release of highly loaded antibodies from functionalized nanoporous support for cancer immunotherapy.
abstract: We report that antibodies can be spontaneously loaded in functionalized mesoporous silica (FMS) with superhigh density (0.4-0.8 mg of antibody/mg of FMS) due to their comprehensive noncovalent interaction. The superhigh loading density and noncovalent interaction between FMS and antibodies allow long-lasting local release of the immunoregulatory molecules from FMS under physiological conditions. Preliminary data indicate that FMS-anti-CTLA4 antibody injected directly into a mouse melanoma induces much greater and extended inhibition of tumor growth than the antibody given systemically. Our findings open up a novel approach for local delivery of therapeutically active proteins to tumors and, potentially, other diseases.
authors.affiliation: Pacific Northwest National Laboratory, Richland, Washington 99352, USA. chenghong.lei@pnl.gov
authors.forename: Chenghong
authors.initials: C
authors.lastname: Lei
authors.forename: Pu
authors.initials: P
authors.lastname: Liu
authors.forename: Baowei
authors.initials: B
authors.lastname: Chen
authors.forename: Yumeng
authors.initials: Y
authors.lastname: Mao
authors.forename: Heather
authors.initials: H
authors.lastname: Engelmann
authors.forename: Yongsoon
authors.initials: Y
authors.lastname: Shin
authors.forename: Jade
authors.initials: J
authors.lastname: Jaffar
authors.forename: Ingegerd
authors.initials: I
authors.lastname: Hellstrom
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Karl Erik
authors.initials: KE
authors.lastname: Hellstrom
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use*; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000627:therapeutic use; D007167:Immunotherapy; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D009369:Neoplasms / Q000628:therapy*; D016062:Porosity; D051381:Rats; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D012822:Silicon Dioxide