Synthesis of magnetic and fluorescent bifunctional nanocomposites and their applications in detection of lung cancer cells in humans.
We developed a novel strategy to detect lung cancer cells by utilizing magnetic and fluorescent bifunctional nanocomposites (BNPs) in combination with monoclonal anti-carcinoembryonic antigen (CEA) antibodies. The BNPs, consisting of silica-coated superparamagnetic nanoparticles and quantum dots (QDs), exhibited high luminescence and were easily separated in an external magnetic field. The binding specificity of the antibody-conjugated BNPs (immunonanoparticles) were confirmed via incubating with human lung adenocarcinoma SPCA-1 cells, human leukemic K562 cells and human embryonic lung fibroblasts MRC-5 cells. Further experiments demonstrated that the as-prepared immunonanoparticles can efficiently capture and detect cancer cells in pleural effusion from lung cancer patients. These results suggest that this method, of which the detection procedures are completed within 1h, could be applied to the rapid and cost-effective monitoring of cancer cells in clinical samples.
Ma
Jingwei
J
Department of Chemistry, Life and Environment Science College, Shanghai Normal University, Shanghai, PR China.
Fan
Qishi
Q
Wang
Lianhui
L
Jia
Nengqin
N
Gu
Zhidong
Z
Shen
Hebai
H
D000328:Adult; D002272:Carcinoembryonic Antigen / Q000737:chemistry; D045744:Cell Line, Tumor; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D020014:K562 Cells; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D008280:Magnetics; D008297:Male; D008875:Middle Aged; D053761:Nanocomposites / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D045663:Quantum Dots; D014376:Tuberculosis / Q000378:metabolism
D002272:Carcinoembryonic Antigen; D005456:Fluorescent Dyes