title: EZYprep LC-coupled MALDI-TOF/TOF MS: an improved matrix spray application for phosphopeptide characterisation.
abstract: The quality of MALDI-TOF mass spectrometric analysis is highly dependent on the matrix and its deposition strategy. Although different matrix-deposition methods have specific advantages, one major problem in the field of proteomics, particularly with respect to quantitation, is reproducibility between users or laboratories. Compounding this is the varying crystal homogeneity of matrices depending on the deposition strategy used. Here, we describe a novel optimised matrix-deposition strategy for LC-MALDI-TOF/TOF MS using an automated instrument that produces a nebulised matrix "mist" under controlled atmospheric conditions. Comparisons of this with previously reported strategies showed the method to be advantageous for the atypical matrix, 2,5-DHB, and improved phosphopeptide ionisation when compared with deposition strategies for CHCA. This optimised DHB matrix-deposition strategy with LC-MALDI-TOF/TOF MS, termed EZYprep LC, was subsequently optimised for phosphoproteome analysis and compared to LC-ESI-IT-MS and a previously reported approach for phosphotyrosine identification and characterisation. These methods were used to map phosphorylation on epidermal growth factor-stimulated epidermal growth factor receptor to gauge the sensitivity of the proposed method. EZYprep DHB LC-MALDI-TOF/TOF MS was able to identify more phosphopeptides and characterise more phosphorylation sites than the other two proteomic strategies, thus proving to be a sensitive approach for phosphoproteome analysis.
authors.affiliation: School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, SA, Australia.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Condina
authors.forename: Johan O R
authors.initials: JO
authors.lastname: Gustafsson
authors.forename: Manuela
authors.initials: M
authors.lastname: Klingler-Hoffmann
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Bagley
authors.forename: Shaun R
authors.initials: SR
authors.lastname: McColl
authors.forename: Peter
authors.initials: P
authors.lastname: Hoffmann
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D066246:ErbB Receptors / Q000032:analysis / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D010748:Phosphopeptides; D066246:ErbB Receptors