Experimental investigation of pulsatility effect on the deformability and hemolysis of blood cells.
In this study, we investigated the differences between pulsatile cardiopulmonary bypass (CPB) procedure and nonpulsatile CPB procedure in terms of their effects on hemolysis and deformability of red blood cells (RBCs) under various shear stress conditions. In order to research the effects on hemolysis and deformability, four parameters--free hemoglobin (fHb) concentration, normalized index of hemolysis (NIH), deformability index (DI) of RBCs, and elongation index of RBCs--have been deeply investigated. For these investigations, two randomly assigned adult mongrel dog groups-nonpulsatile group (NP, n = 6) and pulsatile group (P, n = 6)--were examined. According to our results, both types of perfusion did not show any statistical differences in terms of the concentrations of fHb as well as NIH. In addition, there were no significant differences in RBC deformability between perfusion types within an operation time of 3 h. Therefore, our studies suggest that pulsatile perfusion has no significant difference from nonpulsatile perfusion in terms of hemolysis and deformability of RBCs.
Kang
Yang Jun
YJ
Department of Mechatronics, Gwangju Institute of Science and Technology, Buk-gu, Gwangju, Korea.
Kim
Myoung Gon
MG
Son
Kuk Hui
KH
Lim
Choon Hak
CH
Son
Ho Sung
HS
Yoon
Sang Youl
SY
Kwon
Hyuk Sang
HS
Yang
Sung
S
D000818:Animals; D002315:Cardiopulmonary Bypass / Q000009:adverse effects* / Q000379:methods*; D004285:Dogs; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000166:cytology*; D006461:Hemolysis; D007091:Image Processing, Computer-Assisted; D011673:Pulsatile Flow; D011897:Random Allocation; D018709:Statistics, Nonparametric