The role of CD44 and matrix metalloproteinase-9 expression in predicting neck metastasis of supraglottic laryngeal carcinoma.
OBJECTIVE
The aim of this study is to evaluate the role of CD44 and matrix metalloproteinase (MMP)-9 expression in predicting neck metastasis of supraglottic laryngeal carcinoma.


METHODS
Two hundred ninety-four supraglottic laryngeal cancers were treated surgically from 1991 to 2005. Ninety-four of the 294 patients had pathologically metastatic lymph node (pN+). Among the 94 patients, 30 pN+ patients were selected via random sampling. Sex-, T value-, and differentiation-matched 30 patients who had pathologically negative neck were also selected. CD44 and MMP-9 antibodies were applied to the tumor representative sections that were derived from paraffin sections by using the streptavidin-biotin method. The association between immunohistochemical results and histopathologic lymph node metastasis was analyzed statistically. The association between immunostaining of CD44 and MMP-9 was also analyzed.


RESULTS
Overexpression of CD44 and MMP-9 was found to be significantly higher in pN+ patients. There was fair concordance between immunostaining of CD44 and MMP-9.


CONCLUSIONS
Although wider multiinstitutional and multidisciplinary studies are needed to draw specific conclusions, CD44 and MMP-9 can be useful in the prediction of neck metastasis in the supraglottic laryngeal carcinoma.
Yüce
Imdat
I
Department of Otorhinolaryngology and Head & Neck Surgery, Erciyes University, Kayseri, Turkey. imdatyuce@hotmail.com
Bayram
Ali
A
Cağlı
Sedat
S
Canöz
Ozlem
O
Bayram
Sevgi
S
Güney
Ercihan
E
D014408:Biomarkers, Tumor / Q000378:metabolism*; D016022:Case-Control Studies; D006258:Head and Neck Neoplasms / Q000556:secondary*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D007822:Laryngeal Neoplasms / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged; D011237:Predictive Value of Tests
D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors; D020780:Matrix Metalloproteinase 9