Multicenter collaborative study for standardization of Candida albicans genotyping using a polymorphic microsatellite marker.
Microsatellite-based genotyping for Candida albicans can give discrepant results between laboratories when expressed in fragment sizes, because their determination depends on electrophoretic conditions. The interlaboratory reproducibility was assessed in six laboratories provided with an allelic ladder. Despite variations in size determinations, alleles were correctly assigned, making data transportable between laboratories.
Garcia-Hermoso
Dea
D
Institut Pasteur, Unité de Mycologie Moléculaire, Centre National de Référence Mycologie et Antifongiques and CNRS URA3012, Paris, France. dea.garcia-hermoso@pasteur.fr
MacCallum
Donna M
DM
Lott
Timothy J
TJ
Sampaio
Paula
P
Serna
Maria-José Buitrago
MJ
Grenouillet
Fréderic
F
Klaassen
Corné H W
CH
Bretagne
Stéphane
S
D000483:Alleles; D015373:Bacterial Typing Techniques / Q000379:methods / Q000592:standards; D002176:Candida albicans / Q000235:genetics*; D005838:Genotype; D007753:Laboratories; D018895:Microsatellite Repeats / Q000235:genetics*; D025202:Molecular Diagnostic Techniques / Q000379:methods / Q000592:standards; D011110:Polymorphism, Genetic; D012015:Reference Standards; D015203:Reproducibility of Results