Effects of isolated compounds from Catalpa ovata on the T cell-mediated immune responses and proliferation of leukemic cells.
Three compounds were isolated from the ethyl acetate soluble fraction of the methanolic extract of the leaves of Catalpa ovata (Bignoniaceae) through repeated column chromatography. We investigated the effects of these compounds on T cell-mediated responses for tumor surveillance and proliferation in U937, HL60, and Molt-4 leukemia cells. Compounds 1-3 inhibited proliferation of those cells in a dose-dependent manner. Compound 3 showed mild effect in Molt-4 cell cytotoxicity. Compound 3 enhanced gene expressions of p53 and IL-4, but decreased IL-2 and IFN-Gamma genes in Molt-4 cell. Our findings indicate that compound 3 may enhance T cell-mediated immune responses and anticancer properties.
Oh
Chan-Ho
CH
Department of Food & Biotechnology, Woosuk University, Jeonju, 565-701, Korea.
Kim
Nam-Seok
NS
Yang
Jae Heon
JH
Lee
Hyunjin
H
Yang
Seokwon
S
Park
Saerom
S
So
Un-Ki
UK
Bae
Jin-Beom
JB
Eun
Jae Soon
JS
Jeon
Hoon
H
Lim
Jong Pil
JP
Kwon
Jin
J
Kim
Young-Suk
YS
Shin
Tae Yong
TY
Kim
Dae Keun
DK
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D029402:Bignoniaceae / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects* / Q000276:immunology; D055670:Medicine, Korean Traditional; D015394:Molecular Structure; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D020298:U937 Cells
D000972:Antineoplastic Agents, Phytogenic; D016207:Cytokines; D010936:Plant Extracts