Inhibitory effect of Jeju endemic seaweeds on the production of pro-inflammatory mediators in mouse macrophage cell line RAW 264.7.
Seaweed has been used in traditional cosmetics and as a herbal medicine in treatments for cough, boils, goiters, stomach ailments, and urinary diseases, and for reducing the incidence of tumors, ulcers, and headaches. Despite the fact that seaweeds are frequently used in the practice of human health, little is known about the role of seaweed in the context of inflammation. This study aimed to investigate the influence of Jeju endemic seaweed on a mouse macrophage cell line (RAW 264.7) under the stimulation of lipopolysaccharide (LPS). Ethyl acetate extracts obtained from 14 different kinds of Jeju seaweeds were screened for inhibitory effects on pro-inflammatory mediators. Our results revealed that extracts from five seaweeds, Laurencia okamurae, Grateloupia elliptica, Sargassum thunbergii, Gloiopeltis furcata, and Hizikia fusiformis, were potent inhibitors of the production of pro-inflammatory mediators such as nitric oxide (NO), prostaglandin E(2) (PGE(2)), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha). Based on these results, the anti-inflammatory effects and low cell toxicity of these seaweed extracts suggest potential therapeutic applications in the regulation of the inflammatory response.
Yang
Eun-Jin
EJ
Jeju Biodiversity Research Institute, Jeju High-Tech Development Institute, Jeju 699-943, Korea.
Moon
Ji-Young
JY
Kim
Min-Jin
MJ
Kim
Dong Sam
DS
Kim
Chan-Shick
CS
Lee
Wook Jae
WJ
Lee
Nam Ho
NH
Hyun
Chang-Gu
CG
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D012624:Seaweed / Q000145:classification*
D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D010936:Plant Extracts