title: Identification of protein-bound dinitrosyl iron complexes by nuclear resonance vibrational spectroscopy.
abstract: We have applied (57)Fe nuclear resonance vibrational spectroscopy (NRVS) to identify protein-bound dinitrosyl iron complexes. Intense NRVS peaks due to vibrations of the N-Fe-N unit can be observed between 500 and 700 cm(-1) and are diagnostic indicators of the type of iron dinitrosyl species present. NRVS spectra for four iron dinitrosyl model compounds are presented and used as benchmarks for the identification of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors.forename: Zachary J
authors.initials: ZJ
authors.lastname: Tonzetich
authors.forename: Hongxin
authors.initials: H
authors.lastname: Wang
authors.forename: Devrani
authors.initials: D
authors.lastname: Mitra
authors.forename: Christine E
authors.initials: CE
authors.lastname: Tinberg
authors.forename: Loi H
authors.initials: LH
authors.lastname: Do
authors.forename: Francis E
authors.initials: FE
authors.lastname: Jenney
authors.forename: Michael W W
authors.initials: MW
authors.lastname: Adams
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Cramer
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Lippard
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry / Q000378:metabolism; D005288:Ferredoxins / Q000737:chemistry / Q000378:metabolism; D007501:Iron / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D020027:Pyrococcus furiosus; D013057:Spectrum Analysis; D014732:Vibration
chemical_list: D019843:Archaeal Proteins; D005288:Ferredoxins; D009589:Nitrogen Oxides; D011506:Proteins; C073661:dinitrosyl iron complex; D007501:Iron