Screening of lipases for the synthesis of xylitol monoesters by chemoenzymatic esterification and the potential of microwave and ultrasound irradiations to enhance the reaction rate.
Lipases from different sources, Pseudomonas fluorescens (AK lipase), Burkholderia cepacia (PS lipase), Penicillium camembertii (lipase G) and Porcine pancreas lipase (PPL), previously immobilized on epoxy SiO(2)-PVA, were screened for the synthesis of xylitol monoesters by esterification of the protected xylitol using oleic acid as acyl donor group. Among all immobilized derivatives, the highest esterification yield was achieved by P. camembertii lipase, showing to be attractive alternative to bulk chemical routes to satisfy increasing commercial demands. Further experiments were performed to determine the influence of fatty acids chain size on the reaction yield and the feasibility of using non-conventional heating systems (microwave and ultrasound irradiations) to enhance the reaction rate.
Rufino
Alessandra R
AR
Engineering School of Lorena, University of São Paulo, PO Box 116, 12602-810 Lorena, São Paulo, Brazil.
Biaggio
Francisco C
FC
Santos
Julio C
JC
de Castro
Heizir F
HF
D000818:Animals; D016956:Burkholderia cepacia / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004952:Esters / Q000138:chemical synthesis; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D008872:Microwaves; D009829:Oleic Acids / Q000138:chemical synthesis*; D010407:Penicillium / Q000201:enzymology; D011551:Pseudomonas fluorescens / Q000201:enzymology; D013016:Sound; D013552:Swine; D014993:Xylitol / Q000737:chemistry*
D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins; D009829:Oleic Acids; C093667:Novozyme 435; D008049:Lipase; D014993:Xylitol