Enhancing chemotherapeutic drug inhibition on tumor growth by ultrasound: an in vivo experiment.
An in vivo study on enhancing epirubicin hydrochloride (EPI) inhibition on tumor growth by ultrasound (US) was reported. Five-week-old male nude mice were used and HL-60 cells were s.c. (subcutaneous injection) inoculated in axilla of these mice. Six groups were designed and five consecutive treatments were applied to investigate the inhibition on tumor growth and body weight growth. US applied locally to the tumor resulted in a substantially increased drug uptake in tumor cells. The inhibition on tumor growth depended on the position of drug injection and phospholipid-based microbubble (PMB) application. Tumor growth rate under group 1 (PMB+US) was similar to that of blank control. The order of the inhibition on tumor volume growth was: group 4 (s.c. EPI+PMB+US) > group 5 intraperitoneal (i.p. EPI+PMB+US) > group 2 (i.p. EPI) > group 3 (s.c. EPI+US) > group 1 (PMB+US). Similar conclusion was obtained from experimental measurements of tumor weight change. The order of animal survival status for EPI administration groups was: group 4 > group 5 > group 2 > group 3. Chemotherapeutic drug inhibition on tumor growth could be enhanced by local US combined with PMB, which might provide a potential application for US-mediated chemotherapy.
Zhao
Ying-Zheng
YZ
Pharmacy School, Wenzhou Medical College, Zhejiang Province 325035, China. lctuua@yahoo.com.cn
Lu
Cui-Tao
CT
Zhou
Zhi-Cai
ZC
Jin
Zhuo
Z
Zhang
Lu
L
Sun
Chang-Zheng
CZ
Xu
Yan-Yan
YY
Gao
Hui-Sheng
HS
Tian
Ji-Lai
JL
Gao
Feng-Hou
FH
Tang
Qin-Qin
QQ
Li
Wei
W
Xiang
Qi
Q
Li
Xiao-Kun
XK
Li
Wen-Feng
WF
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D016503:Drug Delivery Systems; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D015251:Epirubicin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D045423:Microbubbles; D010743:Phospholipids / Q000737:chemistry; D013534:Survival; D014465:Ultrasonics / Q000379:methods*
D000903:Antibiotics, Antineoplastic; D010743:Phospholipids; D015251:Epirubicin