title: The effect of the endothelial glycocalyx layer on concentration polarisation of low density lipoprotein in arteries.
abstract: It has been postulated that a flow-dependent (and hence spatially varying) low density lipoprotein (LDL) concentration polarisation layer forms on the luminal surface of the vascular endothelium. Such a layer has the potential to cause heterogeneity in the distribution of atherosclerotic lesions by spatially modulating the rate of LDL transport into the arterial wall. Theoretical analysis suggests that a transmural water flux which is spatially heterogeneous at the cellular scale can act to enhance LDL concentration polarisation in a shear dependent fashion. However, such an effect is only observed if a relevant Peclet number (i.e. the ratio of LDL convection to LDL diffusion) is of order unity or greater. Based on the diffusivity of LDL in blood plasma, such a Peclet number is found to be far less than unity, implying that the aforementioned enhancement and shear dependence will not occur. However, this conclusion ignores the existence of the endothelial glycocalyx layer (EGL), which may inhibit the diffusion of LDL near the luminal surface of the endothelium, and hence raise any Peclet number associated with the transport of LDL. The present study numerically investigates the effect of the EGL, as well as a heterogeneous transmural water flux, on arterial LDL concentration polarisation. Particular attention is paid to measures of LDL concentration polarisation thought relevant to the rate of transendothelial LDL transport. It is demonstrated that an EGL is unlikely to cause any additional shear dependence of such measures directly, irrespective of whether or not LDL can penetrate into the EGL. However, it is found that such measures depend significantly on the nature of the interaction between LDL and the EGL (parameterized by the height of the EGL, the depth to which LDL penetrates into the EGL, and the diffusivity of LDL in the EGL). Various processes may regulate the interaction of LDL with the EGL, possibly in a flow dependent and hence spatially non-uniform fashion. It is concluded that any such processes may be as important as vascular scale flow features in terms of spatially modulating transendothelial LDL transport via an LDL concentration polarisation mechanism.
authors.affiliation: Department of Aeronautics, Imperial College London, South Kensington, London SW7 2AZ, UK. pvincent@stanford.edu
authors.forename: P E
authors.initials: PE
authors.lastname: Vincent
authors.forename: S J
authors.initials: SJ
authors.lastname: Sherwin
authors.forename: P D
authors.initials: PD
authors.lastname: Weinberg
mesh_terms: D000818:Animals; D001158:Arteries / Q000166:cytology* / Q000378:metabolism*; D001692:Biological Transport; D042783:Endothelial Cells / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism*; D019276:Glycocalyx / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood* / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D012212:Rheology; D014867:Water / Q000502:physiology
chemical_list: D008077:Lipoproteins, LDL; D014867:Water