title: Mass spectrometry analysis of the post-translational modifications of alpha-enolase from pancreatic ductal adenocarcinoma cells.
abstract: Enolase is a key glycolytic enzyme that catalyzes the dehydration of 2-phosphoglycerate to phosphoenolpyruvate. Recently, enolase was revealed as an important protein in pathophysiological processes since it was found on the surface of hematopoietic cells and overexpressed in several tumor cells. Our previous studies demonstrated that alpha-enolase is up-regulated in pancreatic ductal adenocarcinoma (PDAC). In this present work, we further characterized the alpha-enolase from PDAC and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap and identified multiple post-translational modifications of alpha-enolase, such as phosphorylation, acetylation, and methylation. The result showed that more acetylated lysines, methylated aspartic acids, and glutamic acids were found in PDAC cells than that of normal pancreatic duct cells.
authors.affiliation: Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, Virginia 20110, USA. wzhou@gmu.edu
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhou
authors.forename: Michela
authors.initials: M
authors.lastname: Capello
authors.forename: Claudia
authors.initials: C
authors.lastname: Fredolini
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Piemonti
authors.forename: Lance A
authors.initials: LA
authors.lastname: Liotta
authors.forename: Francesco
authors.initials: F
authors.lastname: Novelli
authors.forename: Emanuel F
authors.initials: EF
authors.lastname: Petricoin
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D021441:Carcinoma, Pancreatic Ductal / Q000201:enzymology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D010190:Pancreatic Neoplasms / Q000201:enzymology*; D010751:Phosphopyruvate Hydratase / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010751:Phosphopyruvate Hydratase