The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration.
The RNA binding protein Larp1 was originally shown to be involved in spermatogenesis, embryogenesis and cell-cycle progression in Drosophila. Our data show that mammalian Larp1 is found in a complex with poly A binding protein and eukaryote initiation factor 4E and is associated with 60S and 80S ribosomal subunits. A reduction in Larp1 expression by siRNA inhibits global protein synthesis rates and results in mitotic arrest and delayed cell migration. Consistent with these data we show that Larp1 protein is present at the leading edge of migrating cells and interacts directly with cytoskeletal components. Taken together, these data suggest a role for Larp1 in facilitating the synthesis of proteins required for cellular remodelling and migration.
Burrows
Carla
C
Department of Molecular Oncology, Imperial College, Hammersmith Campus, Du Cane Road, London W12 0HS, UK.
Abd Latip
Normala
N
Lam
Sarah-Jane
SJ
Carpenter
Lee
L
Sawicka
Kirsty
K
Tzolovsky
George
G
Gabra
Hani
H
Bushell
Martin
M
Glover
David M
DM
Willis
Anne E
AE
Blagden
Sarah P
SP
D000199:Actins / Q000032:analysis; D017209:Apoptosis; D001324:Autoantigens / Q000378:metabolism / Q000502:physiology*; D002465:Cell Movement; D003598:Cytoskeletal Proteins / Q000378:metabolism; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D010442:Peptide Chain Initiation, Translational; D039102:Poly(A)-Binding Proteins / Q000378:metabolism; D011554:Pseudopodia / Q000737:chemistry / Q000648:ultrastructure; D012261:Ribonucleoproteins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D000097585:SS-B Antigen
D000199:Actins; D001324:Autoantigens; D003598:Cytoskeletal Proteins; D039561:Eukaryotic Initiation Factor-4E; D039102:Poly(A)-Binding Proteins; D012261:Ribonucleoproteins; D000097585:SS-B Antigen