Synthesis and characterization of pH sensitive carboxySNARF-1 nanoreactors.
A rapid response dual wavelength emission pH sensor consisting of carboxySNARF-1 nanoreactors has been synthesized and shown to provide accurate pH measurements even in complex biological media, where the unprotected pH responsive dyes have failed. The carboxySNARF-1 nanoreactor is made of a calcium phosphate shell covering phosphatidylcholine liposomes filled with the dye. Its mean diameter is 150 nm with dynamic light scattering, the shell thickness is 5-7 nm with TEM, and it contains about 10 dyes/particle. The nanoreactor's response time to pH change nearly equals that of the dye in solution. Its pH titration curves at two different wavelengths are equivalent to those of the dye in solution and fluorescence intensity ratio dependent pH analysis is possible using the modified Henderson-Hasselbalch equation. However, the pH dependent fluorescence ratios of the dye in solution in the presence of plasma and albumin are distorted, and application of the Henderson-Hasselbalch equation is not possible. We have found that the distortions may be restored using cSNARF-1 nanoreactors and the pK(a) of the dye in the nanoreactor then equals that in solution. These results suggest that the interference to the dye for the pH analyses with the environmental molecules may be reduced or prohibited by usage of cSNARF-1 nanoreactors.
Chen
Yen-Chi
YC
Department of Materials Science, University of Utah, Salt Lake City, UT, USA.
Ostafin
Agnes
A
Mizukami
Hiroshi
H
D001578:Benzopyrans / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D016014:Linear Models; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D009284:Naphthols / Q000138:chemical synthesis / Q000737:chemistry*; D010316:Particle Size; D038761:Photobleaching; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*
D001578:Benzopyrans; D009284:Naphthols; D012235:Rhodamines; C068301:carboxy-seminaphthorhodaminefluoride