title: Galactosylated chitosan-g-PEI/DNA complexes-loaded poly(organophosphazene) hydrogel as a hepatocyte targeting gene delivery system.
abstract: Hydrogels are widely used in drug delivery systems because they can control the release and thereby enhance the efficiency of locally delivered bioactive molecules such as therapeutic drugs, proteins, or genes. For gene delivery, localized release of plasmid DNA or polymer/DNA complexes can transfect cells and produce sustained protein production. We tested the galactosylated chitosan-graft-polyethylenimine (GC-g-PEI)/DNA complexes-loaded poly(organophosphazene) thermosensitive biodegradable hydrogel as a hepatocyte targeting gene delivery system. The poly(organophosphazene) hydrogel loaded with GC-g-PEI/DNA complexes showed low cytotoxicity and higher transfection efficiency than PEI/DNA complexes, as well as good hepatocyte specificity in vitro and in vivo. Our results indicate that poly(organophosphazene) hydrogels loaded with GC-g-PEI/DNA complexes may be a safe and efficient hepatocyte targeting gene delivery system.
authors.affiliation: College of Veterinary Medicine, Seoul National University, Seoul, 151-742, Korea.
authors.forename: Hu-Lin
authors.initials: HL
authors.lastname: Jiang
authors.forename: You-Kyoung
authors.initials: YK
authors.lastname: Kim
authors.forename: Sun-Mi
authors.initials: SM
authors.lastname: Lee
authors.forename: Mi-Ran
authors.initials: MR
authors.lastname: Park
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Kim
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Jin
authors.forename: Rohidas
authors.initials: R
authors.lastname: Arote
authors.forename: Hwan-Jeong
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Soo-Chang
authors.initials: SC
authors.lastname: Song
authors.forename: Myung-Haing
authors.initials: MH
authors.lastname: Cho
authors.forename: Chong-Su
authors.initials: CS
authors.lastname: Cho
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity; D004247:DNA / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D005260:Female; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D009943:Organophosphorus Compounds / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D011094:Polyethyleneimine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity; D011108:Polymers / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D012995:Solubility; D014162:Transfection; D014783:Viscosity
chemical_list: D004337:Drug Carriers; D020100:Hydrogels; D009943:Organophosphorus Compounds; D011108:Polymers; C551320:chitosan-graft-polyethylenimine; C515246:poly(organophosphazene); D011094:Polyethyleneimine; D004247:DNA; D048271:Chitosan; C427203:calf thymus DNA