Dynamic strength of titin's Z-disk end.
Titin is a giant filamentous protein traversing the half sarcomere of striated muscle with putative functions as diverse as providing structural template, generating elastic response, and sensing and relaying mechanical information. The Z-disk region of titin, which corresponds to the N-terminal end of the molecule, has been thought to be a hot spot for mechanosensing while also serving as anchorage for its sarcomeric attachment. Understanding the mechanics of titin's Z-disk region, particularly under the effect of binding proteins, is of great interest. Here we briefly review recent findings on the structure, molecular associations, and mechanics of titin's Z-disk region. In addition, we report experimental results on the dynamic strength of titin's Z1Z2 domains measured by nanomechanical manipulation of the chemical dimer of a recombinant protein fragment.
Kollár
Veronika
V
Department of Biophysics, Faculty of Medicine, University of Pécs, Szigeti ut 12, Pécs 7624, Hungary.
Szatmári
Dávid
D
Grama
László
L
Kellermayer
Miklós S Z
MS
D001696:Biomechanical Phenomena; D064211:Connectin; D004548:Elasticity; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D009010:Monte Carlo Method; D009124:Muscle Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017510:Protein Folding; D011494:Protein Kinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D033081:Shear Strength
D064211:Connectin; D009124:Muscle Proteins; D011994:Recombinant Proteins; C577292:TCAP protein, human; C577285:TTN protein, human; D011494:Protein Kinases