title: Effects of adenosine receptor antagonists on survival in amitriptyline-poisoned mice.
abstract: OBJECTIVE
  We investigated the effects of adenosine receptor antagonists on survival rates in a mouse model of amitriptyline poisoning.
  
  
  METHODS
  In the preliminary study, amitriptyline was given at doses of 75, 100, and 125 mg/ kg to mice intraperitoneally (i.p.; n = 20 for each dose) to determine the lethal dose at 50% (LD(50)). Different doses (1, 3, and 5 mg/kg) of DPCPX (selective adenosine A(1) antagonists, n = 10 for each dose, total n = 30) or CSC (selective adenosine A(2a) antagonists, n = 10 for each dose, total n = 30) were given i.p. to find the nonlethal dose. After the administration of the LD(50) dose of amitriptyline (125 mg/kg), mice were treated with DPCPX (3 mg/kg), CSC (3 mg/kg), saline, or DMSO (dimethyl sulfoxide) (n = 25 for each group). Mice were observed during a 24-hour period.
  
  
  RESULTS
  Kaplan-Meier estimates of the 24-hour survival rate was 52% (13/25) for saline and 68% (17/25), 52% (13/25), and 40% (10/25) for the DPCPX, CSC, and DMSO groups, respectively. There was no statistically significant difference in survival rates among the groups (P > 0.05).
  
  
  CONCLUSIONS
  Adenosine antagonists failed to increase the survival rates of amitriptyline-poisoned mice. Further studies are needed with repeated doses of adenosine antagonists.
authors.affiliation: Department of Pharmacology, Dokuz Eylul University School of Medicine, Izmir, Turkey. sule.kalkan@deu.edu.tr
authors.forename: Sule
authors.initials: S
authors.lastname: Kalkan
authors.forename: Nil
authors.initials: N
authors.lastname: Hocaoglu
authors.forename: Aylin Akgun
authors.initials: AA
authors.lastname: Arici
authors.forename: Kubilay
authors.initials: K
authors.lastname: Oransay
authors.forename: Gul
authors.initials: G
authors.lastname: Ergor
authors.forename: Yesim
authors.initials: Y
authors.lastname: Tuncok
mesh_terms: D058916:Adenosine A1 Receptor Antagonists; D058917:Adenosine A2 Receptor Antagonists; D000639:Amitriptyline / Q000506:poisoning*; D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000506:poisoning*; D004305:Dose-Response Relationship, Drug; D053208:Kaplan-Meier Estimate; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D043682:Receptor, Adenosine A1 / Q000378:metabolism; D043705:Receptor, Adenosine A2A / Q000378:metabolism; D014970:Xanthines / Q000494:pharmacology*
chemical_list: C515416:8-(3-Chlorostyryl)-1,3,7-trimethylxanthine; D058916:Adenosine A1 Receptor Antagonists; D058917:Adenosine A2 Receptor Antagonists; D000929:Antidepressive Agents, Tricyclic; D043682:Receptor, Adenosine A1; D043705:Receptor, Adenosine A2A; D014970:Xanthines; D000639:Amitriptyline; C051360:1,3-dipropyl-8-cyclopentylxanthine