The absence of cardiomyopathy is accompanied by increased activities of CAT, MnSOD and GST in long-term diabetes in rats.
The activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST), the incidence of DNA damage, the activation of poly (ADP-ribose) polymerase-1 (PARP-1), a marker of DNA repair, and connective tissue growth factor (CTGF), a marker of tissue fibrosis, were examined in the hearts of rats for 16 weeks after diabetes induction by streptozotocin (STZ) administration. A 150% increase in CAT activity was detected at the end of the 2nd week post-STZ administration, and CAT activity remained 80% above the control level throughout 16 weeks. While total SOD and CuZn-SOD exhibited progressively decreasing activities, those of Mn-SOD and GST were elevated. Neither DNA strand breaks (apoptosis or necrosis) nor changes in PARP-1 activity and in CTGF levels (fibrosis) were observed in the diabetic heart. The absence of cardiomyopathy is accompanied with increased activities of CAT, MnSOD and GST.
Ivanović-Matić
Svetlana
S
Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Despot Stephen Blvd. 142, 11060, Belgrade, Serbia. smatic@ibiss.bg.ac.rs
Mihailović
Mirjana
M
Dinić
Svetlana
S
Martinović
Vesna
V
Bogojević
Desanka
D
Grigorov
Ilijana
I
Poznanović
Goran
G
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D009202:Cardiomyopathies / Q000378:metabolism / Q000473:pathology; D002374:Catalase / Q000378:metabolism*; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D004249:DNA Damage; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology* / Q000473:pathology; D005982:Glutathione Transferase / Q000378:metabolism*; D008297:Male; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*
D000975:Antioxidants; C526316:CCN2 protein, rat; D055513:Connective Tissue Growth Factor; D002374:Catalase; D013482:Superoxide Dismutase; C499322:Parp1 protein, rat; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D005982:Glutathione Transferase