A revisit to the one form kinetic model of prothrombinase.
Thrombin is generated enzymatically from prothrombin by two pathways with the intermediates of meizothrombin and prethrombin-2. Experimental concentration profiles from two independent groups for these two pathways have been re-analyzed. By rationally combining the independent data sets, a simple mechanism can be established and rate constants determined. A structural model is consistent with the data-derived finding that mechanisms that feature channeling or ratcheting are not necessary to describe thrombin production.
Lee
Chang Jun
CJ
Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599-3290, USA.
Wu
Sangwook
S
Eun
Changsun
C
Pedersen
Lee G
LG
D001777:Blood Coagulation; D020134:Catalytic Domain; D007700:Kinetics; D056004:Molecular Dynamics Simulation; D017434:Protein Structure, Tertiary; D011516:Prothrombin / Q000737:chemistry / Q000378:metabolism; D013917:Thrombin / Q000737:chemistry / Q000378:metabolism; D013925:Thromboplastin / Q000737:chemistry* / Q000378:metabolism
D011516:Prothrombin; D013925:Thromboplastin; D013917:Thrombin