title: Oxidative stress in mice infected with Angiostrongylus cantonensis coincides with enhanced glutathione-dependent enzymes activity.
abstract: This study aimed to estimate reactive oxygen species (ROS) production, antioxidants activity, and biomarkers level of oxidative damage to protein and DNA in the cerebrospinal fluid (CSF) of C57BL/6 mice infected with Angiostrongylus cantonensis. The mean ROS concentration in the CSF of infected mice increased gradually, and the increase in ROS in CSF became statistical significance at days 12-30 post-infection compared to that before infection (P<0.001), and then ROS returned to normal level at day 45 after infection. In parallel with the increase in ROS in the CSF, infected mice showed similar of changes in reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx), and glutathione S-transferase (GST) as that in ROS in the CSF. GSH, GR, GPx, and GST in the CSF of infected mice were all significantly higher than they were before infection during days 12-30 post-infection. However, protein carbonyl content and 8-hydroxy-2'-deoxyguanosine, biomarkers of oxidative damage to protein and DNA, respectively, were also significantly higher in the CSF of infected mice during this period. These results suggest that oxidative stress occur in the cells of central nervous system of mice infected with A. cantonensis during days 12-30 after infection due to ROS overproduction in CSF despite the increase in antioxidants during this period.
authors.affiliation: Department of Parasitology and Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan, ROC.
authors.forename: Li-Yu
authors.initials: LY
authors.lastname: Chung
authors.forename: Chun-Hsiang
authors.initials: CH
authors.lastname: Chen
authors.forename: Lian-Chen
authors.initials: LC
authors.lastname: Wang
authors.forename: Shun-Jen
authors.initials: SJ
authors.lastname: Chang
authors.forename: Chuan-Min
authors.initials: CM
authors.lastname: Yen
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D017185:Angiostrongylus cantonensis / Q000378:metabolism*; D000818:Animals; D001700:Biomphalaria; D002490:Central Nervous System / Q000201:enzymology* / Q000378:metabolism / Q000469:parasitology; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000134:cerebrospinal fluid; D005978:Glutathione / Q000134:cerebrospinal fluid; D005979:Glutathione Peroxidase / Q000134:cerebrospinal fluid; D005980:Glutathione Reductase / Q000134:cerebrospinal fluid; D005982:Glutathione Transferase / Q000134:cerebrospinal fluid; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000502:physiology*; D050050:Protein Carbonylation; D017382:Reactive Oxygen Species / Q000134:cerebrospinal fluid; D017206:Strongylida Infections / Q000201:enzymology* / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D003849:Deoxyguanosine; D005978:Glutathione