title: [Effect of non-contact coculture on bone marrow stromal cells and neural stem cells].
abstract: OBJECTIVE
  To observe the effect of non-contact coculture on bone marrow stromal cells (MSCs) and neural stem cells (NSCs) in neural stem cell culture medium.
  
  
  METHODS
  MSCs and NSCs were cultured in non-contact coculture in Transwell plate, and cell morphology and immunocytochemical profile were investigated.
  
  
  RESULTS
  In the coculture, the NSCs showed adhering growth and extended long processes, and the migrating cells formed a network of cells within 7 days. The cells differentiated into neurons, astrocytes and oligodendrocytes as shown by immunocytochemistry. Most of the MSCs grew in a non-adherent manner, giving rise to large spherical cell masses which expressed neuronal, astrocyte, and oligodendrocyte phenotypes. In the control group, the NSCs grew in suspension, some of MSCs formed small non-adherent spherical cell masses, while some cells showed adherent growth.
  
  
  CONCLUSIONS
  MSCs and NSCs in the non-contact coculture can mutually promote the cell differentiation into neural cells in neural stem cell culture medium, indicating that both MSCs and NSCs can secrete some neurotrophic factors to provide a microenvironment suitable for survival and differentiation for each other.
authors.affiliation: Department of Neurology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. yangweixiao222@yahoo.com.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Qin
authors.initials: Q
authors.lastname: Yang
authors.forename: Yan-jie
authors.initials: YJ
authors.lastname: Jia
authors.forename: Ao
authors.initials: A
authors.lastname: Li
authors.forename: Zhi-lei
authors.initials: ZL
authors.lastname: Zeng
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
authors.forename: Jun-wei
authors.initials: JW
authors.lastname: Ren
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D005260:Female; D008297:Male; D058953:Neural Stem Cells / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology