A functional carbohydrate chip platform for analysis of carbohydrate-protein interaction.
A carbohydrate chip based on glass or other transparent surfaces has been suggested as a potential tool for high-throughput analysis of carbohydrate-protein interactions. Here we proposed a facile, efficient, and cost-effective method whereby diverse carbohydrate types are modified in a single step and directly immobilized onto a glass surface, with retention of functional orientation. We modified various types of carbohydrates by reductive amination, in which reducing sugar groups were coupled with 4-(2-aminoethyl)aniline, which has di-amine groups at both ends. The modified carbohydrates were covalently attached to an amino-reactive NHS-activated glass surface by formation of stable amide bonds. This proposed method was applied for efficient construction of a carbohydrate microarray to analyze carbohydrate-protein interactions. The carbohydrate chip prepared using our method can be successfully used in diverse biomimetic studies of carbohydrates, including carbohydrate-biomolecule interactions, and carbohydrate sensor chip or microarray development for diagnosis and screening.
Seo
Jeong Hyun
JH
Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Kim
Chang Sup
CS
Hwang
Byeong Hee
BH
Cha
Hyung Joon
HJ
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001427:Bacterial Toxins / Q000737:chemistry / Q000378:metabolism; D002241:Carbohydrates / Q000138:chemical synthesis / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D037102:Lectins / Q000737:chemistry; D046228:Microarray Analysis / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014734:Vibrio cholerae / Q000737:chemistry / Q000378:metabolism
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D002241:Carbohydrates; C519498:GM1 pentasaccharide; D037102:Lectins; D009844:Oligosaccharides; D011506:Proteins