title: ODDSOC2 is a MADS box floral repressor that is down-regulated by vernalization in temperate cereals.
abstract: In temperate cereals, such as wheat (Triticum aestivum) and barley (Hordeum vulgare), the transition to reproductive development can be accelerated by prolonged exposure to cold (vernalization). We examined the role of the grass-specific MADS box gene ODDSOC2 (OS2) in the vernalization response in cereals. The barley OS2 gene (HvOS2) is expressed in leaves and shoot apices but is repressed by vernalization. Vernalization represses OS2 independently of VERNALIZATION1 (VRN1) in a VRN1 deletion mutant of einkorn wheat (Triticum monococcum), but VRN1 is required to maintain down-regulation of OS2 in vernalized plants. Furthermore, barleys that carry active alleles of the VRN1 gene (HvVRN1) have reduced expression of HvOS2, suggesting that HvVRN1 down-regulates HvOS2 during development. Overexpression of HvOS2 delayed flowering and reduced spike, stem, and leaf length in transgenic barley plants. Plants overexpressing HvOS2 showed reduced expression of barley homologs of the Arabidopsis (Arabidopsis thaliana) gene FLOWERING PROMOTING FACTOR1 (FPF1) and increased expression of RNase-S-like genes. FPF1 promotes floral development and enhances cell elongation, so down-regulation of FPF1-like genes might explain the phenotypes of HvOS2 overexpression lines. We present an extended model of the genetic pathways controlling vernalization-induced flowering in cereals, which describes the regulatory relationships between VRN1, OS2, and FPF1-like genes. Overall, these findings highlight differences and similarities between the vernalization responses of temperate cereals and the model plant Arabidopsis.
authors.affiliation: Research School of Biology, Australian National University, Canberra, Australian Capital Territory 0200, Australia.
authors.forename: Aaron G
authors.initials: AG
authors.lastname: Greenup
authors.forename: Shahryar
authors.initials: S
authors.lastname: Sasani
authors.forename: Sandra N
authors.initials: SN
authors.lastname: Oliver
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Talbot
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Dennis
authors.forename: Megan N
authors.initials: MN
authors.lastname: Hemming
authors.forename: Ben
authors.initials: B
authors.lastname: Trevaskis
mesh_terms: D029681:Arabidopsis Proteins / Q000737:chemistry; D002980:Climate; D003080:Cold Temperature; D015536:Down-Regulation / Q000235:genetics*; D002523:Edible Grain / Q000166:cytology / Q000235:genetics / Q000502:physiology; D035264:Flowers / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D006657:Histones / Q000378:metabolism; D001467:Hordeum / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D008239:Lysine / Q000378:metabolism; D026161:MADS Domain Proteins / Q000378:metabolism*; D008745:Methylation; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010641:Phenotype; D017440:Photoperiod; D018515:Plant Leaves / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018547:Plant Stems / Q000254:growth & development; D011401:Promoter Regions, Genetic / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; C108185:FPF1 protein, Arabidopsis; D006657:Histones; D026161:MADS Domain Proteins; D010940:Plant Proteins; D012097:Repressor Proteins; D008239:Lysine