title: Suppression of CDK2 expression by siRNA induces cell cycle arrest and cell proliferation inhibition in human cancer cells.
abstract: Cyclin-dependent kinase 2 (CDK2) is a member of serine/ threonine protein kinases, which initiates the principal transitions of the eukaryotic cell cycle and is a promising target for cancer therapy. The present study was designed to inhibit cdk2 gene expression to induce cell cycle arrest and cell proliferation suppression. Here, we constructed a series of RNA interference (RNAi) plasmids which can successfully express small interference RNA (siRNA) in the transfected human cells. The results showed that the RNAi plasmids containing the coding sequences for siRNAs down-regulated the cdk2 gene expression in human cancer cells at the mRNA and the protein levels. Furthermore, we found that the cell cycle was arrested at G0G1 phases and the cell proliferation was inhibited by different siRNAs. These results demonstrate that suppression of CDK2 activity by RNAi may be an effective strategy for gene therapy in human cancers.
authors.affiliation: School of Medicine, Ningbo University, 818 Fenghua Road, Ningbo 315211, People's Republic of China.
authors.forename: Xiang-E
authors.initials: XE
authors.lastname: Long
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Gong
authors.forename: Lin
authors.initials: L
authors.lastname: Pan
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Zhong
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Le
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.forename: Jun-Ming
authors.initials: JM
authors.lastname: Guo
authors.forename: Jiu-Chang
authors.initials: JC
authors.lastname: Zhong
mesh_terms: D049109:Cell Proliferation; D051357:Cyclin-Dependent Kinase 2 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D016193:G1 Phase; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000201:enzymology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D016192:Resting Phase, Cell Cycle; D014407:Tumor Cells, Cultured
chemical_list: D034741:RNA, Small Interfering; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2