Structural and kinetic molecular dynamics study of electroporation in cholesterol-containing bilayers.
We present a numerical study of pore formation in lipid bilayers containing cholesterol (Chol) and subjected to a transverse electric field. Molecular dynamics simulations of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DOPC) membranes reveal the formation of a pore when an electric field of 325 mV/nm is applied. The minimum electric field needed for membrane permeabilization strongly increases with the addition of cholesterol above 10 mol %, reaching 750 mV/nm for 40 mol % Chol. Analysis of simulations of DOPC/Chol bilayers suggests this is caused by a substantial increment of membrane cohesion. Simulations also show that pore formation kinetics is much slower at high Chol contents.
Fernández
M Laura
ML
Laboratorio de Sistemas Complejos, Departamento de Computación, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina.
Marshall
Guillermo
G
Sagués
Francesc
F
Reigada
Ramon
R
D002784:Cholesterol / Q000737:chemistry*; D018274:Electroporation; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D010713:Phosphatidylcholines / Q000737:chemistry
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D002784:Cholesterol; C017251:1,2-oleoylphosphatidylcholine