[Experimental study on avascular necrosis of femoral head in chickens induced by different glucocorticoides].
OBJECTIVE
To study the effects of Methylprednisolone and Dexamethasone on the avascular necrosis of femoral head in chickens.


METHODS
Thirty-six chickens were randomly divided into 6 groups (n = 6): control group (group A), Methylprednisolone low dose group (group B), Methylprednisolone large dose group (group C), small dose Dexamethasone and horse serum group (group D), middle dose Dexamethasone and horse serum group (group E), and Dexamethasone large dose group (group F). On the 6th and 12th weeks, blood samples were obtained to determine the level of total cholesterol triglyeride (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL). On the 12th week, femoral heads were taken off. Paraffin tissue sections were prepared to detect histopathologic change with hematoxylin and eosin staining.


RESULTS
On the 6th week, compared with group A, the level of CHO increased significantly in group C and group F (P < 0.05), and TG increased in group B, C and group E, while HDL decreased in group B, C and group E. On the 12th week, the level of TG and CHO increased in group B, C, E and group F, and HDL decreased in group C, D and group E (P < 0.05). LDL was not detected in most chickens. The ratio of empty lacuna was higher in group C and group E compared with those of the control group (P < 0.05).


CONCLUSIONS
Methylprednisolone is easier to induce osteonecrosis of femoral head than Dexamethasone. The condition of metabolic disorder in blood may be the basic pathomechanism of steroid-induced necrosis of femoral head.
Xiao
Chun-Sheng
CS
Wangling Hospital Affiliated to China Academy of Chinese Medical Science, Beijing 100102, China.
Lin
Na
N
Lin
Shi-Fu
SF
Wan
Rong
R
Chen
Wei-Heng
WH
D000818:Animals; D002645:Chickens; D002784:Cholesterol / Q000097:blood; D003907:Dexamethasone / Q000009:adverse effects*; D004195:Disease Models, Animal; D005271:Femur Head Necrosis / Q000097:blood / Q000139:chemically induced*; D005938:Glucocorticoids / Q000009:adverse effects*; D006801:Humans; D008775:Methylprednisolone / Q000009:adverse effects*; D009842:Oligopeptides / Q000097:blood
D005938:Glucocorticoids; D009842:Oligopeptides; D003907:Dexamethasone; D002784:Cholesterol; C035647:glycyl-glycyl-glycine; D008775:Methylprednisolone