Amperometric glucose biosensor based on biocompatible poly(dimethylaminoethyl) methacrylate microparticles.
Glucose oxidase (GOx) has been immobilized within poly(dimethylamino) ethyl methacrylate (p-DMAEM) microparticles which were subsequently used as biological material in the fabrication of a glucose biosensor. The enzyme immobilization method was optimized in relation with the monomer concentration and cross-linker content. It was found that the best biosensor response corresponds to microparticles synthesized with 1.19M monomer and 0.37% cross-linking content. Furthermore, the influence on the biosensor response of parameters such as working potential, pH, temperature, and loaded enzyme were investigated. In addition, analytical properties such as sensitivity, linear range, response time, and detection limit were determined. The biosensor was used to analyze glucose in human serum samples with satisfactory results. The useful lifetime of the biosensor is at least 520 days.
Hervás Pérez
J P
JP
Sección Departamental de Química Analítica, Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria s/n, Madrid, Spain.
López-Cabarcos
E
E
López-Ruiz
B
B
D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation* / Q000379:methods; D003432:Cross-Linking Reagents; D004563:Electrochemistry / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009757:Nylons; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors
D001786:Blood Glucose; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D008689:Methacrylates; D009757:Nylons; D011108:Polymers; C407037:poly(2-(dimethylamino)ethyl methacrylate); D005949:Glucose Oxidase