Identification of glucosylceramides containing sphingatrienine in maize and rice using ion trap mass spectrometry.
We characterized the glucosylceramide moieties from maize and rice using liquid chromatography-ion trap mass spectrometry. Glucosylceramides containing 4,8-sphingadienine (d18:2) acylated to hydroxy-fatty acids were detected as the predominant molecules both in maize and in rice. In addition, 4-hydroxy-8-sphingenine (t18:1) and sphingatrienine (d18:3) were found in maize and rice glucosylceramides, and in the case of rice, sphingenine (d18:1) was also detected. Glucosylceramides containing d18:3 were acylated to hydroxyl fatty acids (16-24 carbon atoms). Our results indicate the presence of the triene type of sphingoid base in higher plants.
Sugawara
Tatsuya
T
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan. sugawara@kais.kyoto-u.ac.jp
Duan
Jingjing
J
Aida
Kazuhiko
K
Tsuduki
Tsuyoshi
T
Hirata
Takashi
T
D002851:Chromatography, High Pressure Liquid; D005963:Glucosylceramides / Q000032:analysis / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013110:Sphingosine / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
D005963:Glucosylceramides; D013110:Sphingosine