Practical method for extraction of PCR-quality DNA from environmental soil samples.
Methods for the extraction of PCR-quality DNA from environmental soil samples by using pairs of commercially available kits were evaluated. Coxiella burnetii DNA was detected in spiked soil samples at <1,000 genome equivalents per gram of soil and in 12 (16.4%) of 73 environmental soil samples.
Fitzpatrick
Kelly A
KA
Centers for Disease Control and Prevention, MS G-13, 1600 Clifton Rd., Atlanta, GA 30333, USA.
Kersh
Gilbert J
GJ
Massung
Robert F
RF
D016997:Coxiella burnetii / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic; D012987:Soil