Random mutagenesis of Clostridium cellulolyticum by using a Tn1545 derivative.
Further understanding of the plant cell wall degradation system of Clostridium cellulolyticum and the possibility of metabolic engineering in this species highlight the need for a means of random mutagenesis. Here, we report the construction of a Tn1545-derived delivery tool which allows monocopy random insertion within the genome.
Blouzard
Jean-Charles
JC
Centre National de la Recherche Scientifique, IFR88, UPR 9043, Laboratoire de Chimie Bactérienne, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France.
Valette
Odile
O
Tardif
Chantal
C
de Philip
Pascale
P
D046973:Ruminiclostridium cellulolyticum / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D019426:Integrases / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000379:methods*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D019426:Integrases