Determination of clonal status of pulmonary sclerosing hemangioma with X-chromosome inactivation mosaicism and polymorphism of phosphoglycerate kinase and androgen receptor genes.
It has been reported that both polygonal and cuboidal cells in pulmonary sclerosing hemangioma (PSH) are monoclonal in origin and represent a variable differentiation from a common progenitor cell. However, it remains unclear about the clonality of the entire PSH lesion composed of these two types of cells. Thus, we analyzed the clonality of 22 cases of PSH and the relationship between the entire PSH and two types of cells using laser microdissection and X-chromosomal inactivation mosaicism and polymorphism at the phosphoglycerate kinase (PGK) and androgen receptor (AR) loci in female somatic cells. The results demonstrated all 22 cases of PSH showed typical histopathologic characteristics, including characteristic round or polygonal cells within the stroma and surface cuboidal cells lining the papillary projections or cystic spaces. The rates of polymorphism were 31.8% (7/22) and 86.3% (19/22) for the PGK and AR gene, respectively. After digestion by Hpa II or Hha I, one of two PCR amplification bands disappeared from all the samples, while the other band was retained, indicating neoplastic characteristics. Thus, we concluded that the entire PSH lesion, polygonal and cuboidal cells were neoplastic hyperplasia and originated from a common progenitor cell.
Gong
Li
L
The Helmholtz Sino-German Research Laboratory for Cancer, Department of Pathology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, 710038, People's Republic of China.
Ren
Kai-Xi
KX
Li
Yan-Hong
YH
Liu
Xiao-Yan
XY
Zhang
Wen-Dong
WD
Yao
Li
L
Zhu
Shao-Jun
SJ
Han
Xiu-Juan
XJ
Zhang
Li
L
Lan
Miao
M
Zhang
Wei
W
D019070:Cell Lineage / Q000235:genetics; D002999:Clone Cells; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007834:Lasers; D042282:Microdissection; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D047868:Pulmonary Sclerosing Hemangioma / Q000235:genetics*; D011944:Receptors, Androgen / Q000235:genetics*; D049951:X Chromosome Inactivation / Q000235:genetics*
D011944:Receptors, Androgen; D010735:Phosphoglycerate Kinase