Visualization of G-quadruplexes by using a BODIPY-labeled macrocyclic heptaoxazole.
A BODIPY-labeled macrocyclic heptaoxazole, L1BOD-7OTD, was developed as a fluorescent ligand for G-quadruplexes. The results of the study show that L1BOD-7OTD both selectively induces the formation of intramolecular G-quadruplexes from some G-quadruplex forming oligonucleotides (GFOs). In addition, the labelled macrocyclic heptaozaxole strongly binds to and stabilizes intramolecular G-quadruplexes. Moreover, this substance can be used to directly visualize the G-quadruplexes in the form of green fluorescence. Finally, the possibility that G-quadruplexes form in the cells was demonstrated by using of L1BOD-7OTD.
Tera
Masayuki
M
Department of Biotechnology and Life Science Faculty of Technology, Tokyo University of Agriculture and Technology (TUAT), Koganei, Tokyo 184-8588, Japan.
Iida
Keisuke
K
Ikebukuro
Kazunori
K
Seimiya
Hiroyuki
H
Shin-Ya
Kazuo
K
Nagasawa
Kazuo
K
D001896:Boron Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D047028:Macrocyclic Compounds / Q000138:chemical synthesis; D008958:Models, Molecular; D009841:Oligonucleotides / Q000235:genetics; D010080:Oxazoles / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D008024:Ligands; D047028:Macrocyclic Compounds; D009841:Oligonucleotides; D010080:Oxazoles