title: A major subgroup of Beijing family Mycobacterium tuberculosis is associated with multidrug resistance and increased transmissibility.
abstract: This study investigated further the association between the Beijing family Mycobacterium tuberculosis circulating in rural China and anti-tuberculosis (TB) drug resistance. In total, 351 M. tuberculosis isolates were collected through a population-based epidemiological study, 223 (63·5%) of which were resistant to at least one anti-TB drug, including 53 (15·1%) multidrug-resistant (MDR) isolates. Spoligotyping found 243 isolates (69·2%) that belonged to the Beijing family. A major subgroup of the Beijing family identified by mycobacterial interspersed repetitive unit (MIRU) genotyping (223325173533), showed significantly higher frequencies of MDR (44·7% vs. 13·7%, OR 6·18, 95% CI 2·68-14·23), katG and rpoB mutations (31·6% vs. 9·3%, OR 4·27, 95% CI 1·86-9·80), and being clustered by IS6110 RFLP genotyping (60·5% vs. 21·0%, OR 6·14, 95% CI 2·82-13·37) in comparison with other Beijing family isolates. Our data suggest that MIRU genotype 223325173533 of the Beijing family is associated with MDR and increased transmissibility.
authors.affiliation: School of Public Health, Fudan University, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.forename: X
authors.initials: X
authors.lastname: Ma
authors.forename: E A
authors.initials: EA
authors.lastname: Graviss
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Jiang
authors.forename: B
authors.initials: B
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics; D002374:Catalase / Q000235:genetics; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D012321:DNA-Directed RNA Polymerases; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects* / Q000235:genetics; D016017:Odds Ratio; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission*; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; C505805:rpoB protein, Mycobacterium tuberculosis; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases