title: Calcium ion propagation in cultured keratinocytes and other cells in skin in response to hydraulic pressure stimulation.
abstract: We have previously suggested that a variety of environmental factors might be first sensed by epidermal keratinocytes, which represent the frontier of the body. To further examine this idea, in the present study, we examined the intracellular calcium responses of cultured keratinocytes to external hydraulic pressure. First, we compared the responses of undifferentiated and differentiated keratinocytes with those of fibroblasts, vascular endothelial cells (VEC), and lymphatic endothelial cells. Elevation of intracellular calcium was observed after application of pressure to keratinocytes, fibroblasts, and VEC. The calcium propagation extended over a larger area and continued for a longer period of time in differentiated keratinocytes, as compared with the other cells. The response of the keratinocytes was dramatically reduced when the cells were incubated in medium without calcium. Application of a non-selective transient receptor potential (TRP) channel blocker also attenuated the calcium response. These results suggest that differentiated keratinocytes are sensitive to external pressure and that TRP might be involved in the mechanism of their response.
authors.affiliation: Shiseido Research Center, Kanazawa-ku, Yokohama, Japan. makiko.goto@to.shiseido.co.jp
authors.forename: Makiko
authors.initials: M
authors.lastname: Goto
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Ikeyama
authors.forename: Moe
authors.initials: M
authors.lastname: Tsutsumi
authors.forename: Sumiko
authors.initials: S
authors.lastname: Denda
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Denda
mesh_terms: D020013:Calcium Signaling / Q000187:drug effects; D002454:Cell Differentiation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism; D004729:Endothelium, Lymphatic / Q000166:cytology / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism*; D040542:Mechanotransduction, Cellular / Q000187:drug effects; D049990:Membrane Transport Modulators / Q000494:pharmacology; D011312:Pressure; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D050052:TRPC Cation Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013997:Time Factors
chemical_list: D049990:Membrane Transport Modulators; D050052:TRPC Cation Channels