title: Effect of hepatic blood inflow occlusion without hemihepatic artery control on apoptosis of liver cells in rats.
abstract: OBJECTIVE
  To investigate the effect of hepatic blood inflow occlusion without hemihepatic artery control on the apoptosis of liver cells during ischemia-reperfusion injury in rats.
  
  
  METHODS
  Animal models of three hepatic vascular occlusion methods were established. Hepatic blood inflow occlusion without hemihepatic artery control (WH group), hemihepatic vascular occlusion (HH group) and Pringle maneuver (PR group) were compared.
  
  
  RESULTS
  At each time point after reperfusion, ALT, AST, and apoptosis rates were significantly higher in the PR group than in the HH group or the WH group (p < 0.01), and the pathology and hepatocyte ultrastructure at 6 hours after reperfusion were significantly worse in the PR group than in the HH group or the WH group (p < 0.01). ALT, AST, apoptosis rate, pathology and hepatocyte ultrastructure at 6 hours after reperfusion were not significantly different between the WH group and the HH group (p > 0.05).
  
  
  CONCLUSIONS
  Hepatic blood inflow occlusion without hemihepatic artery control can reduce hepatic ischemia-reperfusion injury, and the effect is similar to that of hemihepatic vascular occlusion.
authors.affiliation: Department of General Surgery, Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010050, China. altten_aull@yahoo.com.cn
authors.forename: Shan
authors.initials: S
authors.lastname: Jin
authors.forename: Chaoliu
authors.initials: C
authors.lastname: Dai
authors.forename: Yuntian
authors.initials: Y
authors.lastname: Yang
authors.forename: Ruiming
authors.initials: R
authors.lastname: Zhang
authors.forename: A
authors.initials: A
authors.lastname: Sileng
mesh_terms: D000818:Animals; D017209:Apoptosis; D003250:Constriction; D004195:Disease Models, Animal; D004210:Dissection; D006488:Hemostasis, Surgical; D006498:Hepatectomy / Q000009:adverse effects / Q000379:methods*; D006499:Hepatic Artery; D022781:Hepatocytes / Q000473:pathology* / Q000648:ultrastructure; D011169:Portal Vein; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology* / Q000517:prevention & control*