WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro.
OBJECTIVE
To investigate the mode of action of WSS45, one sulfated derivative of an alpha-D-glucan from the Gastrodia elata Bl, on the multiplication cycle of dengue virus serotype 2 (DV2), including initial infection and intracellular replication.


METHODS
Virus multiplication in BHK cells were monitored by qRT-PCR, plaque reduction assay, and flow cytometry. Initial virus infection was dissected into adsorption and penetration steps by converting temperature and treating by acid glycine. Surface bound virions were detected by immunofluorescence staining for Evelope protein.


RESULTS
WSS45 effectively inhibited DV2 infection in BHK cells with an EC(50) value of 0.68+/-0.17 microg/mL, mainly interfered with virus adsorption, in a very early stage of the virus cycle. However, WSS45 showed no viricidal effect. Moreover, WSS45 could increase the detaching of virus from cell surface in BHK cell line.


CONCLUSIONS
WSS45 exerted potent inhibitory effect on DV2 through interfering with the interaction between viruses and targeted cells. This activity was related to its molecular size.
Tong
Xian-kun
XK
Laboratory of Immunopharmacology and Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Qiu
Hong
H
Zhang
Xin
X
Shi
Li-ping
LP
Wang
Gui-feng
GF
Ji
Fei-hong
FH
Ding
Hui-yong
HY
Tang
Wei
W
Ding
Kan
K
Zuo
Jian-ping
JP
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003715:Dengue / Q000188:drug therapy*; D003716:Dengue Virus / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D031668:Gastrodia / Q000737:chemistry*; D005936:Glucans / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D005936:Glucans; C078962:glucan sulfate