[Effect of Ganoderma lucidum polysaccharides on hemodynamic and antioxidation in T2DM rats].
OBJECTIVE
To investigate the effect of Ganoderma lucidum polysaccharides (GLPs) on hemodynamic and antioxidation in the T2DM rats.


METHODS
SD rats were fed high-fat diet for 4 weeks and then were injected STZ (30 mg x kg(-1)) to induce the type 2 diabetes mellitus (T2DM). Once the T2DM models were set successfully, rats were randomized into six groups: normal group (NG), group of diabetes mellitus (DMG), groups of low dosage (GLPs-LG), middle dosage (GLPs-MG), high dosage (GLPs-HG) and berberine (BerG). They received GLPs with different dosages (200, 400, 800 mg x kg(-1)) and berberine (30 mg x kg(-1)) continually for 16 weeks. At 16th weekend, the following indices of rats were measured respectively: blood glucose, hemodynamic including LVSP, LVEDP, dp/dt(max) and -dp/dt(max) and the contents of NO, SOD, MDA, GSH-Px, CAT in cardiac tissue. Besides, myocardial ultrastructure was observed by electron microscope.


RESULTS
Both the middle dosage and the high dosage of GLPs could low blood glucose effectively, and they could reduce LVEP but increase -dp/dt(max). Meanwhile, they could activate GSH-Px, CAT, SOD, NO, but reduce MDA in cardiac tissue and improve the myocardial ultrastructure. Compared to the DM group, the middle dosage, high dosage of GLPs and berberine showed significant improvement. Compared to the berberine group, the middle dosage showed the same effect, but the high dosage was more effective than berberine.


CONCLUSIONS
There is a confirmed action of GLPs in improving the hemodynamic and antioxidation in cardiac tissue of T2DM rats.
Xue
Hua
H
Department of Pharmacology, Medical College, Nantong University, Nantong 226001, China. xuehua19841028@hotmail.com
Qiao
Jin
J
Meng
Guoliang
G
Wu
Feng
F
Luo
Jia
J
Chen
Hui
H
Zheng
Huihua
H
Xu
Jiliang
J
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006439:Hemodynamics / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000627:therapeutic use; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D009569:Nitric Oxide / Q000378:metabolism; D011134:Polysaccharides / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020940:Reishi / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D007004:Hypoglycemic Agents; D011134:Polysaccharides; D009569:Nitric Oxide; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione