Postnatal development of neurons, interneurons and glial cells in the substantia nigra of mice.
We investigated postnatal alterations of neurons, interneurons and glial cells in the mouse substantia nigra using immunohistochemistry. Tyrosine hydroxylase (TH), neuronal nuclei (NeuN), parvalbumin (PV), neuronal nitric oxide synthase (nNOS), glial fibrillary acidic protein (GFAP), ionized calcium-binding adaptor molecule 1 (Iba 1), CNPase (2',3'-cyclic nucleotide 3'-phosphodiesterase), brain-derived neurotrophic factor (BDNF) and glial cell-line-derived neurotrophic factor (GDNF) immunoreactivity were measured in 1-, 2-, 4- and 8-week-old mice. In the present study, the maturation of NeuN-immunopositive neurons preceded the production of TH in the substantia nigra during postnatal development in mice. Furthermore, the maturation of nNOS-immunopositive interneurons preceded the maturation of PV-immunopositive interneurons in the substantia nigra during postnatal development. Among astrocytes, microglia and oligodendrocytes, in contrast, the development process of oligodendrocytes is delayed in the substantia nigra. Our double-labeled immunohistochemical study suggests that the neurotrophic factors such as BDNF and GDNF secreted by GFAP-positive astrocytes may play some role in maturation of neurons, interneurons and glial cells of the substantia nigra during postnatal development in mice. Thus, our findings provide valuable information on the development processes of the substantia nigra.
Abe
Manami
M
Department of Neurobiology and Therapeutics, Graduate School and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78, Sho-machi, Tokushima, 770-8505, Japan.
Kimoto
Hiroki
H
Eto
Risa
R
Sasaki
Taeko
T
Kato
Hiroyuki
H
Kasahara
Jiro
J
Araki
Tsutomu
T
D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D000818:Animals; D000831:Animals, Newborn; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D004268:DNA-Binding Proteins; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007395:Interneurons / Q000166:cytology* / Q000201:enzymology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology* / Q000201:enzymology; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010320:Parvalbumins / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D013194:Staining and Labeling; D013378:Substantia Nigra / Q000166:cytology* / Q000201:enzymology / Q000254:growth & development*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
C491049:Aif1 protein, mouse; D019208:Brain-Derived Neurotrophic Factor; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D051100:Glial Cell Line-Derived Neurotrophic Factor; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D009687:Nuclear Proteins; D010320:Parvalbumins; D052248:Nitric Oxide Synthase Type I; D014446:Tyrosine 3-Monooxygenase; D010727:Phosphoric Diester Hydrolases; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; C571338:Cnp protein, mouse