title: Effect of jasmonic acid-methyl ester on the composition of carbohydrates and germination of yellow lupine (Lupinus luteus L.) seeds.
abstract: Mature seeds of yellow lupine contained sucrose, raffinose family oligosaccharides (RFOs), and galactosyl cyclitols as major soluble carbohydrates. The study showed that RFOs dominated in lupine seeds (16% DW). The disappearance of both types of alpha-d-galactosides in germinating lupine seeds was strongly inhibited by the presence of jasmonic acid-methyl ester (JA-Me) at a concentration of 10(-3)M in the incubation medium. JA-Me inhibited the activity of alpha-D-galactosidase (fraction I) during seed germination. Anatomical studies of lupine roots have shown certain cell structure differences between control and JA-Me-treated seedlings. The cross-sections of plant roots treated with JA-Me showed a characteristic folding of the cell walls in all root tissues, starting from the rhyzodermis, cortex and vascular cylinder. In water-treated (control) plants, the cell walls were rounded with no folding.
authors.affiliation: Department of Biochemistry, University of Warmia and Mazury, Olsztyn, Poland. k.zalewski@uwm.edu.pl
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Zalewski
authors.forename: Bartosz
authors.initials: B
authors.lastname: Nitkiewicz
authors.forename: Lesław B
authors.initials: LB
authors.lastname: Lahuta
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Głowacka
authors.forename: Aleksander
authors.initials: A
authors.lastname: Socha
authors.forename: Ryszard
authors.initials: R
authors.lastname: Amarowicz
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D002241:Carbohydrates / Q000032:analysis*; D003517:Cyclopentanes / Q000494:pharmacology*; D005696:Galactosidases / Q000378:metabolism; D018525:Germination / Q000187:drug effects*; D031297:Lupinus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D054883:Oxylipins / Q000494:pharmacology*; D010936:Plant Extracts / Q000378:metabolism; D018517:Plant Roots / Q000033:anatomy & histology / Q000166:cytology / Q000187:drug effects; D011887:Raffinose / Q000378:metabolism; D012639:Seeds / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D000085:Acetates; D002241:Carbohydrates; D003517:Cyclopentanes; D054883:Oxylipins; D010936:Plant Extracts; C011006:jasmonic acid; C072239:methyl jasmonate; D005696:Galactosidases; D011887:Raffinose