title: Teicoplanin-loaded borate bioactive glass implants for treating chronic bone infection in a rabbit tibia osteomyelitis model.
abstract: The treatment of chronic osteomyelitis (bone infection) remains a clinical challenge. In this work, pellets composed of a chitosan-bonded mixture of borate bioactive glass particles (<50microm) and teicoplanin powder (antibiotic), were evaluated in vitro and in vivo for treating chronic osteomyelitis induced by methicillin-resistant Staphylococcus aureus (MRSA) in a rabbit model. When immersed in phosphate-buffered saline, the pellets showed sustained release of teicoplanin over 20-30 days, while the bioactive glass converted to hydroxyapatite (HA) within 7 days, eventually forming a porous HA structure. Implantation of the teicoplanin-loaded pellets in a rabbit tibia osteomyelitis model resulted in the detection of teicoplanin in the blood for about 9 days. The implants converted to a bone-like HA graft, and supported the ingrowth of new bone into the tibia defects within 12 weeks of implantation. Microbiological, histological and scanning electron microscopy techniques showed that the implants provided a cure for the bone infection. The results indicate that the teicoplanin-loaded borate bioactive glass implant, combining sustained drug release with the ability to support new bone ingrowth, could provide a method for treating chronic osteomyelitis.
authors.affiliation: Institute of Bioengineering & Information Technology Materials, Tongji University, Shanghai 200092, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Weitao
authors.initials: W
authors.lastname: Jia
authors.forename: Yifei
authors.initials: Y
authors.lastname: Gu
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Deping
authors.initials: D
authors.lastname: Wang
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhang
authors.forename: Wenhai
authors.initials: W
authors.lastname: Huang
authors.forename: Mohamed N
authors.initials: MN
authors.lastname: Rahaman
authors.forename: Delbert E
authors.initials: DE
authors.lastname: Day
authors.forename: Nai
authors.initials: N
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D001881:Borates / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry; D002908:Chronic Disease; D004343:Drug Implants / Q000737:chemistry*; D005260:Female; D005898:Glass / Q000737:chemistry*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D010019:Osteomyelitis / Q000188:drug therapy*; D011817:Rabbits; D013203:Staphylococcal Infections / Q000188:drug therapy; D017334:Teicoplanin / Q000008:administration & dosage* / Q000627:therapeutic use; D013977:Tibia / Q000187:drug effects / Q000473:pathology
chemical_list: D000900:Anti-Bacterial Agents; D001881:Borates; D004343:Drug Implants; D017334:Teicoplanin; D048271:Chitosan