Calcineurin promotes proliferation, migration, and invasion of small cell lung cancer.
A novel role for calcineurin (Cn) has been reported recently regarding the oncogenic potential in pancreatic and colorectal cancer. The aim of this study was to investigate the putative causal role calcineurin could play in the development of lung cancer with bone metastases. We found that CnAalpha, an isoform of calcineurin, was significantly overexpressed in lung cancer tissues with bone metastasis as compared to tumors with non-bone metastases as investigated by RT-PCR. Strong nuclear staining of tumor cells was observed in small cell lung cancer tissues with bone metastasis. Conversely, cytoplasmic staining of tumor cells was observed in small cell lung cancer tissues with non-bone metastasis. Western blots of nuclear proteins from lung cancer tissues indicated that CnAalpha was highly expressed in lung cancer tissues with bone metastases, but not in those with non-bone metastases. In vitro, it was demonstrated that the CnAalpha gene obviously promoted cell proliferation and inhibited cell apotosis. The CnAalpha gene affected the cell cycle and promoted G1[Symbol: see text]S transition in SBC-3 cells. Transfection with the CnAalpha gene promoted cell migration and invasion. These results indicated that CnAalpha may affect the biological behavior of the human small cell lung cancer cell line SBC-3 in vitro and may be a candidate tumor promotor gene for developing bone metastases.
Liu
Yan
Y
Department of Oncology, Tangdu Hospital, Fourth Military Medical University, No.1 Xinsi Road, Ba Qiao District, Xi'an, 710038, Shaanxi Province, China.
Zhang
Ye
Y
Min
Jie
J
Liu
Li-Li
LL
Ma
Ning-Qiang
NQ
Feng
Ying-Ming
YM
Liu
Dong
D
Wang
Ping-zhong
PZ
Huang
De-Dong
DD
Zhuang
Yan
Y
Zhang
He-Long
HL
D001859:Bone Neoplasms / Q000556:secondary; D019703:Calcineurin / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D055752:Small Cell Lung Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D019703:Calcineurin