title: Aggressive nasal-type natural killer/T-cell lymphoma associated with Epstein Barr virus presenting as testicular tumor.
abstract: BACKGROUND
  Testicular lymphoma is a lethal disease with a median survival of approximately 12 to 24 months. Nasal-type natural killer/T-cell lymphoma of the testis is exceptional whether as a primary or secondary tumor.
  
  
  OBJECTIVE
  The authors report on the comprehensive histopathologic, immunohistochemical and molecular analysis of a case of primary testicular nasal type NK/T cell lymphoma and review the features of previously reported cases.
  
  
  METHODS
  We report a case of primary nasal-type natural killer/T-cell lymphoma of testis in a 28-year-old male. The histopathological examination of the surgical specimen, showed a large lymphoma cells with angioinvasion expressing CD3 (cytoplasmic), CD2, CD8, CD43, CD45, CD45Ro, CD56, T-cell intracellular antigen-1, perforine, Mib1 and granzyme. In situ hybridation for Epstein-Barr-virus- encoded mRNA was positive. Polymerase chain reaction study of formalin-fixed tissue showed lack of T-cell receptor gene rearrangements. The initial stage was I (EA) of Ann Arbor. This lymphoma was refractory to chemotherapy. The patient developed lymph node metastases in the out iliac and in the subclavicular region two months later. He died of disease after eight months.
  
  
  CONCLUSIONS
  This study confirms that testicular NK/T-cell lymphoma deserves to be distinguished from the other testicular lymphomas. In fact, this lymphoma tends to occur at young age, to disseminate early, to have an aggressive course, and is strongly associated with EBV.
authors.affiliation: Department of Pathology. Habib Bourguiba University Hospital, Sfax, Tunisia.
authors.forename: Lobna
authors.initials: L
authors.lastname: Ayadi
authors.forename: Saloua
authors.initials: S
authors.lastname: Makni
authors.forename: Nabil
authors.initials: N
authors.lastname: Toumi
authors.forename: Sameh
authors.initials: S
authors.lastname: Hammami
authors.forename: Slim
authors.initials: S
authors.lastname: Charfi
authors.forename: Mounir
authors.initials: M
authors.lastname: Frikha
authors.forename: Abdelmajid
authors.initials: A
authors.lastname: Khabir
authors.forename: Tahya
authors.initials: T
authors.lastname: Boudawara
mesh_terms: D000328:Adult; D017809:Fatal Outcome; D004854:Herpesvirus 4, Human; D006801:Humans; D054391:Lymphoma, Extranodal NK-T-Cell / Q000473:pathology* / Q000821:virology*; D008297:Male; D012367:RNA, Viral / Q000032:analysis; D013736:Testicular Neoplasms / Q000473:pathology*
chemical_list: D012367:RNA, Viral