title: Phosphorylation pattern of the NDUFS4 subunit of complex I of the mammalian respiratory chain.
abstract: The NDUFS4 subunit of complex I of the mammalian respiratory chain has a fully conserved carboxy-terminus with a canonical RVSTK phosphorylation site. Immunochemical analysis with specific antibodies shows that the serine in this site of the protein is natively present in complex I in both the phosphorylated and non-phosphorylated state. Two-dimensional IEF/SDS-PAGE electrophoresis, (32)P labelling and immunodetection show that "in vitro" PKA phosphorylates the serine in the C-terminus of the NDUFS4 subunit in isolated bovine complex I. (32)P labelling and TLC phosphoaminoacid mapping show that PKA phosphorylates serine and threonine residues in the purified heterologous human NDUFS4 protein.
authors.affiliation: Department of Medical Biochemistry, Biology and Physics (DIBIFIM), University of Bari, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: De Rasmo
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Palmisano
authors.forename: Salvatore
authors.initials: S
authors.lastname: Scacco
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Technikova-Dobrova
authors.forename: Damiano
authors.initials: D
authors.lastname: Panelli
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cocco
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Sardanelli
authors.forename: Antonio
authors.initials: A
authors.lastname: Gnoni
authors.forename: Loris
authors.initials: L
authors.lastname: Micelli
authors.forename: Antonio
authors.initials: A
authors.lastname: Trani
authors.forename: Aldo
authors.initials: A
authors.lastname: Di Luccia
authors.forename: Sergio
authors.initials: S
authors.lastname: Papa
mesh_terms: D000818:Animals; D002417:Cattle; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004579:Electron Transport; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007120:Immunochemistry; D009245:NADH Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D021122:Protein Subunits / Q000302:isolation & purification / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods
chemical_list: D010761:Phosphorus Radioisotopes; D021122:Protein Subunits; D009245:NADH Dehydrogenase; D017868:Cyclic AMP-Dependent Protein Kinases