Pharmacokinetic differences in exposure to camphor after intraruminal dosing in selectively bred lines of goats.
A pharmacokinetic dosing study with camphor was used to determine whether selection lines of high-juniper-consuming goats (HJC, n = 12) and low-juniper-consuming goats (LJC, n = 12) differed in their respective disposition kinetics. Postdosing plasma camphor concentrations were used to examine whether a timed single blood sample collected after intraruminal administration of camphor would be a useful screening test to aid in the identification of HJC. Yearling female Boer x Spanish goats (n = 24) received a single intraruminal dose of monoterpene cocktail (0.270 g/kg of BW) containing 4 different monoterpenes that represented their composition previously reported for Ashe juniper (Juniperus ashei). Camphor, the predominant monoterpene in Ashe juniper, was 49.6% of the mix and was the monoterpene analyzed for this study. Blood samples were taken at 15 time points from 0 to 8 h after dosing. Concentrations of camphor were measured in plasma using solid phase extraction and gas chromatography/flame-ionization detection analysis. Maximal plasma concentration of camphor was greater for LJC than HJC (P = 0.01), and area under the curve extrapolated to infinity was greater for LJC than HJC (P < 0.01). Total systemic exposure (area under the curve) to camphor was 5 times less in HJC goats. We conclude that 1) HJC goats possess internal mechanisms to reduce the bioavailability of camphor, and 2) a blood sample taken at 45 min or at 60 min after intraruminal administration of camphor may be useful for identifying HJC individual animals from within large populations of goats.
Campbell
E J
EJ
Texas AgriLife Research Center, Sonora, TX 76950, USA. erika@sonoratx.net
Frost
R A
RA
Mosley
T K
TK
Mosley
J C
JC
Lupton
C J
CJ
Taylor
C A
CA
Walker
J W
JW
Waldron
D F
DF
Musser
J
J
D000818:Animals; D001947:Breeding; D002164:Camphor / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D004777:Environment; D005247:Feeding Behavior; D005260:Female; D005502:Food; D006041:Goats / Q000378:metabolism*; D018653:Juniperus; D012417:Rumen; D013045:Species Specificity
D002164:Camphor