Serological evidence of bluetongue and a preliminary entomological study in southern Croatia.
In December 2001, bluetongue (BT) was confirmed serologically by the Croatian Veterinary Institute using the competitive enzyme-linked immunosorbent assay (c-ELISA). Results of the serological testing of blood samples from ruminants in the Dubrovacko-Neretvanska County are presented (3,318 sera of ruminants from 53 herds were examined). In total, 357 bovine sera (178 or 49.9% positive), 1,268 ovine sera (174 or 13.7% positive) and 1,693 caprine sera (270 or 15.9% positive) were tested. Antibodies to BT virus serotype 9 were detected in 212 of the positive sera by serum neutralisation. A preliminary light-trap survey for midges of the Culicoides genus was also performed in the Dubrovacko-Neretvanska County. Fourteen light-trap collections from seven locations were examined and yielded a total of 4,872 Culicoides of which 4,492 (92%) belonged to the Obsoletus Complex (including C. obsoletus and C. scoticus).
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Croatian Veterinary Institute, Institute Split, Poljicka cesta 33, Split, Croatia.
Bosnić
S
S
Benić
M
M
Lojkić
M
M
Cac
Z
Z
Cvetnić
Z
Z
Madić
J
J
Separović
S
S
Labrović
A
A
Savini
G
G
Goffredo
M
M