Phosphorylation pattern of the NDUFS4 subunit of complex I of the mammalian respiratory chain.
The NDUFS4 subunit of complex I of the mammalian respiratory chain has a fully conserved carboxy-terminus with a canonical RVSTK phosphorylation site. Immunochemical analysis with specific antibodies shows that the serine in this site of the protein is natively present in complex I in both the phosphorylated and non-phosphorylated state. Two-dimensional IEF/SDS-PAGE electrophoresis, (32)P labelling and immunodetection show that "in vitro" PKA phosphorylates the serine in the C-terminus of the NDUFS4 subunit in isolated bovine complex I. (32)P labelling and TLC phosphoaminoacid mapping show that PKA phosphorylates serine and threonine residues in the purified heterologous human NDUFS4 protein.
De Rasmo
Domenico
D
Department of Medical Biochemistry, Biology and Physics (DIBIFIM), University of Bari, Italy.
Palmisano
Giuseppe
G
Scacco
Salvatore
S
Technikova-Dobrova
Zuzana
Z
Panelli
Damiano
D
Cocco
Tiziana
T
Sardanelli
Anna Maria
AM
Gnoni
Antonio
A
Micelli
Loris
L
Trani
Antonio
A
Di Luccia
Aldo
A
Papa
Sergio
S
D000818:Animals; D002417:Cattle; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004579:Electron Transport; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007120:Immunochemistry; D009245:NADH Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D021122:Protein Subunits / Q000302:isolation & purification / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods
D010761:Phosphorus Radioisotopes; D021122:Protein Subunits; D009245:NADH Dehydrogenase; D017868:Cyclic AMP-Dependent Protein Kinases