title: Unique multipotent cells in adult human mesenchymal cell populations.
abstract: We found adult human stem cells that can generate, from a single cell, cells with the characteristics of the three germ layers. The cells are stress-tolerant and can be isolated from cultured skin fibroblasts or bone marrow stromal cells, or directly from bone marrow aspirates. These cells can self-renew; form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency; and can differentiate into endodermal, ectodermal, and mesodermal cells both in vitro and in vivo. When transplanted into immunodeficient mice by local or i.v. injection, the cells integrated into damaged skin, muscle, or liver and differentiated into cytokeratin 14-, dystrophin-, or albumin-positive cells in the respective tissues. Furthermore, they can be efficiently isolated as SSEA-3(+) cells. Unlike authentic ES cells, their proliferation activity is not very high and they do not form teratomas in immunodeficient mouse testes. Thus, nontumorigenic stem cells with the ability to generate the multiple cell types of the three germ layers can be obtained through easily accessible adult human mesenchymal cells without introducing exogenous genes. These unique cells will be beneficial for cell-based therapy and biomedical research.
authors.affiliation: Department of Stem Cell Biology and Histology, Graduate School of Medicine, Tohoku University, Sendai 980-8575, Japan.
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Kuroda
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kitada
authors.forename: Shohei
authors.initials: S
authors.lastname: Wakao
authors.forename: Kouki
authors.initials: K
authors.lastname: Nishikawa
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Tanimura
authors.forename: Hideki
authors.initials: H
authors.lastname: Makinoshima
authors.forename: Makoto
authors.initials: M
authors.lastname: Goda
authors.forename: Hideo
authors.initials: H
authors.lastname: Akashi
authors.forename: Ayumu
authors.initials: A
authors.lastname: Inutsuka
authors.forename: Akira
authors.initials: A
authors.lastname: Niwa
authors.forename: Taeko
authors.initials: T
authors.lastname: Shigemoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nabeshima
authors.forename: Tatsutoshi
authors.initials: T
authors.lastname: Nakahata
authors.forename: Yo-Ichi
authors.initials: Y
authors.lastname: Nabeshima
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Fujiyoshi
authors.forename: Mari
authors.initials: M
authors.lastname: Dezawa
mesh_terms: D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002449:Cell Aggregation; D002454:Cell Differentiation; D049109:Cell Proliferation; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology*