title: Evaluation of methicillin resistance in Staphylococcus intermedius isolated from dogs.
abstract: Methicillin and multi-drug resistance were investigated in 136 Staphylococcus intermedius strains of canine origin. The large majority of isolates were found to be mecA-negative by polymerase chain reaction, whereas only four strains were positive for the mecA gene. All mecA-positive strains were confirmed as methicillin-resistant by complementary tests, except for oxacillin disk diffusion, which yielded one false-negative result. A significantly higher resistance to fusidic acid, lincosamides, and cotrimoxazole was observed in methicillin-resistant S. intermedius (MRSI) compared with methicillin-susceptible strains. Although the prevalence of MRSI in dogs appeared to be low, methicillin resistance was confirmed to be associated with multi-drug resistance, suggesting the importance of antimicrobial susceptibility testing of canine S. intermedius strains.
authors.affiliation: Dipartimento di Clinica Veterinaria, Università di Pisa, Pisa, Italy. vmeucci@vet.unipi.it
authors.forename: V
authors.initials: V
authors.lastname: Meucci
authors.forename: M
authors.initials: M
authors.lastname: Vanni
authors.forename: L
authors.initials: L
authors.lastname: Guardabassi
authors.forename: A
authors.initials: A
authors.lastname: Moodley
authors.forename: G
authors.initials: G
authors.lastname: Soldani
authors.forename: L
authors.initials: L
authors.lastname: Intorre
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D016106:Methicillin Resistance; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D057789:Staphylococcus intermedius / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents