title: Hsa-mir-182 suppresses lung tumorigenesis through down regulation of RGS17 expression in vitro.
abstract: Lung cancer is one of the most devastating diseases worldwide. RGS17 is previously shown to be over-expressed in human lung adenocarcinomas and plays an important role in lung tumor growth. Here we have identified a miRNA, has-mir-182, involved in the regulation of RGS17 expression through two conserved sites located in its 3' UTR region. Consistently, endogenous RGS17 expression level is regulated by hsa-mir-182 in human lung cancer cell lines. Similar to the knockdown of RGS17, ectopic expression of hsa-mir-182 significantly inhibits lung cancer cell proliferation and anchorage-independent cell growth, which can be rescued by re-expression of RGS17. Taken together, these data have provided the first evidence of miRNA regulation of RGS17 expression in lung cancer.
authors.affiliation: Department of Thoracic Surgery, Fudan University, Shanghai Cancer Center, Shanghai Medical College, Fudan University, Shanghai 200032, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Sun
authors.forename: Rong
authors.initials: R
authors.lastname: Fang
authors.forename: Chenguang
authors.initials: C
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Ye
authors.forename: Haiquan
authors.initials: H
authors.lastname: Chen
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020710:RGS Proteins / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; C538984:Mirn182 microRNA, human; D020710:RGS Proteins; C550555:RGS17 protein, human