Real-time electrochemical monitoring of brain tissue oxygen: a surrogate for functional magnetic resonance imaging in rodents.
Long-term in-vivo electrochemistry (LIVE) enables real-time monitoring and measurement of brain metabolites. In this study we have simultaneously obtained blood oxygenation level dependent (BOLD) fMRI and amperometric tissue O(2) data from rat cerebral cortex, during both increases and decreases in inspired O(2) content. BOLD and tissue O(2) measurements demonstrated close correlation (r=0.7898) during complete (0%) O(2) removal, with marked negative responses occurring ca. 30s after the onset of O(2) removal. Conversely, when the inspired O(2) was increased (50, 70 and 100% O(2) for 1min) similar positive rapid changes (ca. 15s) in both the BOLD and tissue O(2) signals were observed. These findings demonstrate, for the first time, the practical feasibility of obtaining real-time metabolite information during fMRI acquisition, and that tissue O(2) concentration monitored using an O(2) sensor can serve as an index of changes in the magnitude of the BOLD response. As LIVE O(2) sensors can be used in awake animals performing specific behavioural tasks the technique provides a viable animal surrogate of human fMRI experimentation.
Lowry
John P
JP
Department of Chemistry, National University of Ireland, Maynooth, Co. Kildare, Ireland. John.Lowry@nuim.ie
Griffin
Karen
K
McHugh
Stephen B
SB
Lowe
Andrew S
AS
Tricklebank
Mark
M
Sibson
Nicola R
NR
D000818:Animals; D001921:Brain / Q000098:blood supply; D001923:Brain Chemistry; D001931:Brain Mapping / Q000379:methods*; D002560:Cerebrovascular Circulation; D055664:Electrochemical Techniques / Q000379:methods*; D004567:Electrodes, Implanted; D005240:Feasibility Studies; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D010100:Oxygen / Q000097:blood / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D010100:Oxygen