Isolation and characterization of biogenic amine-producing bacteria in fermented soybean pastes.
Biogenic amines (BAs) are produced primarily by microorganisms found in fermented foods and are often implicated in food poisoning. BA-producing bacteria found in fermented soybean pastes were isolated and characterized using a decarboxylating medium and multiplex PCR analysis. Two BA-producing bacteria were isolated from traditional soybean pastes: one was a histamine-producing Clostridium strain, and the other was a tyramine-producing Pseudomonas strain. The Clostridium strain was determined to be a potent histamine producer among the cultures tested. Synthesis of tyramine by Pseudomonas sp. T1 was observed for the first time in this study.
Moon
Jin Seok
JS
Department of Food Science and Technology, Research Center for Bio Resource and Health, Chungbuk National University, Cheongju, 361-763, Republic of Korea.
Cho
Seung Kee
SK
Choi
Hwa Young
HY
Kim
Ji Eun
JE
Kim
So-Young
SY
Cho
Kyung-Ju
KJ
Han
Nam Soo
NS
D001431:Bacteriological Techniques / Q000379:methods; D003013:Clostridium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005285:Fermentation; D006632:Histamine / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000382:microbiology*; D014439:Tyramine / Q000096:biosynthesis*
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D006632:Histamine; D014439:Tyramine