The role of NO and cGMP in antispasmodic activity of Ruta chalepensis leaf extract on rat ileum.
The aim of present study was to investigate the effect of hydroalcoholic extract of Ruta chalepensis (Rutaceae) leaves on rat ileum contractility and possible mechanism(s) involved. Ruta chalepensis extract was prepared by maceration method (ethanol 70%). Terminal portion of ileum (2 cm) was dissected out from male Wistar rats and mounted in an organ bath containing air bubbled Tyrode solution with 1 g initial tension and ileal contraction induced by KCl (60 mM) was recorded. The spasmolytic effect of the cumulative concentrations of extracts (0.01-0.07 mg mL(-1)) was reduced after tissue incubation with L-NAME (100 microM, 20 min). Methylene blue (30 microM) reduced the extracts (0.01-0.07 mg mL(-1)) spasmolytic effect (p < 0.001). Furthermore, it seems that the portion relaxatory effect of Rue extract on the rat ileum may be due to nitric oxide and the antispasmodic activity of the extract was mainly through a cGMP-dependent mechanism.
Moazedi
A A
AA
Department of Biology, School of Science, Shahid Chamran University, Ahwaz, Iran.
Dabir
N
N
Naseri
M K Gharib
MK
Zadkarami
M R
MR
D000818:Animals; D006152:Cyclic GMP / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects / Q000503:physiopathology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology*; D010276:Parasympatholytics / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D032105:Ruta / Q000033:anatomy & histology / Q000737:chemistry*
D004791:Enzyme Inhibitors; D010276:Parasympatholytics; D010936:Plant Extracts; D009569:Nitric Oxide; D011189:Potassium Chloride; D006152:Cyclic GMP; D019331:NG-Nitroarginine Methyl Ester