One-generation reproductive toxicity study of epichlorohydrin in Sprague-Dawley rats.
This study was conducted to evaluate the potential reproductive toxicity of epichlorohydrin in a one-generation reproduction toxicity study in compliance with OECD Test Guideline 415. Twenty-four male and female rats per group were given epichlorohydrin by gavage at 0, 3.3, 10, and 30 mg/kg/day. Males were dosed for 10 weeks prior to and during mating. Females were dosed from 2 weeks before mating to day 21 of lactation. At 30 mg/kg, an increase in the incidence of clinical signs (i.e., nasal discharge, soft feces, depression, and piloerection), gross necropsy findings (i.e., cystic pustule of the epididymidis and enlargement of the kidney) and the weights of heart, liver, and epididymidis, a decrease in male fertility, and an increased incidence of histopathological changes of the testis, epididymidis, and kidney were observed. At 10 mg/kg, decreased male fertility and increased kidney weight and incidence of histopathological changes of the epididymidis were found. There was a slight, but nonsignificant, reduction in the male fertility index at the dose of 3.3 mg/ kg. Under these experimental conditions, the lowest-observed-adverse-effect level of epichlorohydrin was 3.3 mg/kg/day for parent animals and their offspring. The absolute toxic dose for parent animals and their offspring was estimated to be 10 mg/kg/day.
Shin
In-Sik
IS
Animal Medical Center, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
Park
Na-Hyeong
NH
Lee
Jong-Chan
JC
Kim
Kang-Hyeon
KH
Moon
Changjong
C
Kim
Sung-Ho
SH
Shin
Dong-Ho
DH
Park
Seung-Chun
SC
Kim
Hyeon-Young
HY
Kim
Jong-Choon
JC
D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal; D001835:Body Weight; D004781:Environmental Exposure; D004811:Epichlorohydrin / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D006651:Histocytochemistry; D008297:Male; D019055:No-Observed-Adverse-Effect Level; D009929:Organ Size; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects*
D004811:Epichlorohydrin