title: Microfluidic Western blot.
abstract: We develop a novel method for Western blot based on microfluidics, incorporating the internal molecular weight marker, loading control, and antibody titration in the same protocol. Compared with the conventional method which could detect only one protein, the microfluidic Western blot could analyze at least 10 proteins simultaneously from a single sample, and it requires only about 1% of the amount of antibody used in conventional Western blot.
authors.forename: Wenying
authors.initials: W
authors.lastname: Pan
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000906:Antibodies; D015415:Biomarkers / Q000032:analysis*; D015153:Blotting, Western / Q000379:methods*; D044085:Microfluidics / Q000379:methods*; D011506:Proteins / Q000032:analysis*
chemical_list: D000906:Antibodies; D015415:Biomarkers; D011506:Proteins