Roles of charge interactions on astringency of whey proteins at low pH.
Whey proteins are a major ingredient in sports drink and functional beverages. At low pH, whey proteins are astringent, which may be undesirable in some applications. Understanding the astringency mechanism of whey proteins at low pH could lead to developing ways to minimize the astringency. This study compared the astringency of beta-lactoglobulin (beta-LG) at low pH with phosphate buffer controls having the same amount of phosphate and at similar pH. Results showed that beta-LG samples were more astringent than phosphate buffers, indicating that astringency was not caused by acid alone and that proteins contribute to astringency. When comparing among various whey protein isolates (WPI) and lactoferrin at pH 3.5, 4.5, and 7.0, lactoferrin was astringent at pH 7.0 where no acid was added. In contrast, astringency of all WPI decreased at pH 7.0. This can be explained by lactoferrin remaining positively charged at pH 7.0 and able to interact with negatively charged saliva proteins, whereas the negatively charged WPI would not interact. Charge interactions were further supported by beta-LG or lactoferrin and salivary proteins precipitating when mixed at conditions where beta-LG, lactoferrin, or saliva themselves did not precipitate. It can be concluded that interactions between positively charged whey proteins and salivary proteins play a role in astringency of proteins at low pH.
Vardhanabhuti
B
B
Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, NC 27695, USA. bvardha@ncsu.edu
Kelly
M A
MA
Luck
P J
PJ
Drake
M A
MA
Foegeding
E A
EA
D000328:Adult; D002021:Buffers; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007781:Lactoferrin / Q000737:chemistry; D007782:Lactoglobulins / Q000737:chemistry; D008297:Male; D008894:Milk Proteins / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry; D013649:Taste; D000067816:Whey Proteins; D055815:Young Adult
D002021:Buffers; D007782:Lactoglobulins; D008894:Milk Proteins; D000067816:Whey Proteins; D007781:Lactoferrin