Molecularly imprinted polymer-based chemiluminescence array sensor for the detection of proline.
A new molecularly imprinted polymer (MIP)-chemiluminescence (CL) method has been developed for detection of proline. The molecularly imprinted polymer microspheres were synthesized using precipitation polymerization with hydroxyproline, a structural analogue, as the template. Polymer microspheres were immobilized in microtiter plates (96 wells) which selectively adsorbed the analyte (dansyl-proline). After washing, the bound fraction was quantified based on peroxyoxalate chemiluminescence (PO-CL) reaction enhanced by imidazole. The cavity of MIP synthesized with hydroxyproline as template is smaller, which can avoid non-specific adsorption and lead to enhancement of specificity, response speed and sensitivity when recognizing dansyl-proline. Under the optimum conditions, the relative CL intensity has a linear relationship with the concentration of proline in the range of 1x10(-6) mol L(-1) to 4x10(-5) mol L(-1), with a limit of detection 3x10(-7) mol L(-1) (3sigma). The relative standard deviation (R.S.D.) for proline (1x10(-6) mol L(-1), n=7) was 3.7%. The MIP-CL method can become a useful analytical technology for determination of proline in real sample.
Chang
Pingping
P
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China.
Zhang
Zhujun
Z
Yang
Chunyan
C
D000327:Adsorption; D011232:Chemical Precipitation; D008163:Luminescent Measurements / Q000191:economics / Q000295:instrumentation*; D008863:Microspheres; D054802:Molecular Imprinting; D010070:Oxalates / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis*; D011392:Proline / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D010070:Oxalates; D011108:Polymers; C044622:peroxyoxalate; D011392:Proline