Occurrence and composition of class 1 and class 2 integrons in clinical and environmental O1 and non-O1/non-O139 Vibrio cholerae strains from the Brazilian Amazon.
This study identified and characterised class 1 and 2 integrons in clinical and environmental Vibrio cholerae O1 and non-O1/non-O139 strains isolated from the Brazilian Amazon. The aadA2 and aadA7 gene cassettes were found in class 1 integrons in two genotypes of environmental V. cholerae non-O1/non-O139. Empty integrons were found in strains from the Brazilian cholera epidemic. A class 2 integron was detected in one strain from the V. cholerae Amazonia lineage harbouring sat1 and aadA1 genes. All isolates were resistant to aminoglycosides, indicating aadA functionality. These findings suggest that environmental bacteria act as cassette reservoirs that favour the emergence of resistant pathogens.
Sá
Lena Lillian Canto de
LL
Laboratório de Microbiologia Ambiental, Seção de Meio Ambiente, Laboratório de Microbiologia Ambiental, Instituto Evandro Chagas, Belém, PA, Brasil.
Fonseca
Erica Lourenço da
EL
Pellegrini
Maíra
M
Freitas
Fernanda
F
Loureiro
Edvaldo Carlos Brito
EC
Vicente
Ana Carolina Paulo
AC
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil; D002771:Cholera / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D032023:Integrons / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D014734:Vibrio cholerae / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D014871:Water Microbiology
D000900:Anti-Bacterial Agents