Screening and evaluation of human intestinal lactobacilli for the development of novel gastrointestinal probiotics.
The aim of this study was to screen intestinal lactobacilli strains for their advantageous properties to select those that could be used for the development of novel gastrointestinal probiotics. Ninety-three isolates were subjected to screening procedures. Fifty-nine percent of the examined lactobacilli showed the ability to auto-aggregate, 97% tolerated a high concentration of bile (2% w/v), 50% survived for 4 h at pH 3.0, and all strains were unaffected by a high concentration of pancreatin (0.5% w/v). One Lactobacillus buchneri strain was resistant to tetracycline. None of the tested strains caused lysis of human erythrocytes. Six potential probiotic strains were selected for safety evaluation in a mouse model. Five of 6 strains caused no translocation, and were considered safe. In conclusion, several strains belonging to different species and fermentation groups were found that have properties required for a potential probiotic strain. This study was the first phase of a multi-phase study aimed to develop a novel, safe and efficient prophylactic and therapeutic treatment system against gastrointestinal infections using genetically modified probiotic lactobacilli.
Kõll
Piret
P
Department of Microbiology, University of Tartu, Tartu, Estonia.
Mändar
Reet
R
Smidt
Imbi
I
Hütt
Pirje
P
Truusalu
Kai
K
Mikelsaar
Raik-Hiio
RH
Shchepetova
Jelena
J
Krogh-Andersen
Kasper
K
Marcotte
Harold
H
Hammarström
Lennart
L
Mikelsaar
Marika
M
D000143:Acids / Q000633:toxicity; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity; D018988:Bacterial Translocation; D001646:Bile / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004912:Erythrocytes / Q000382:microbiology; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D010194:Pancreatin / Q000633:toxicity; D019936:Probiotics; D017422:Sequence Analysis, DNA
D000143:Acids; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D010194:Pancreatin