Lipopolysaccaride-binding peptides obtained by phage display method.
Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria. It has strong toxicity and might cause sepsis or septic shock. Thus early detection of LPS and neutralization of LPS toxicity are required. We obtained several new LPS-binding peptides using a phage display method. We synthesized 3 of these peptides and analyzed their binding affinity and capacity to LPS. One of these peptides, named Li5-001, showed high binding affinity to LPS and lipid A; the K(d) values were 10 and 1 nM, respectively. Li5-001 showed a high binding capacity to LPS, and was estimated to bind 130 ng LPS/mg, which is higher than that of polymyxin B (80 ng LPS/mg); however, its LPS-neutralizing activity was low. Li5-001 coupled with beads will be useful for eliminating endotoxin contamination from pharmaceuticals. Its low LPS-neutralizing activity allows to be used in the Limulus amebocyte lysate test without eluting LPS from the Li5-001 coupled beads.
Matsumoto
Megumi
M
Peptide Door Co. Ltd., Sangyo-sozo-kan 204, Shimonojo-machi 936-14, Takasaki, Gunma 370-0854, Japan.
Horiuchi
Yoshinobu
Y
Yamamoto
Akihiko
A
Ochiai
Masaki
M
Niwa
Makoto
M
Takagi
Takashi
T
Omi
Hiroyuki
H
Kobayashi
Tomomi
T
Suzuki
Masatsugu-Matt
MM
D001681:Biological Assay / Q000379:methods*; D004731:Endotoxins / Q000737:chemistry* / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000737:chemistry* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism
D004731:Endotoxins; D008070:Lipopolysaccharides; D019151:Peptide Library; D010455:Peptides