title: Friction transfer of polytetrafluoroethylene (PTFE) to produce nanoscale features and influence cellular response in vitro.
abstract: A large number of cell types are known to respond to chemical and topographical patterning of substrates. Friction transfer of polytetrafluoroethylene (PTFE) onto substrates has been shown to produce continuous, straight, parallel nanofibres. Ammonia plasma treatment can be used to defluorinate the PTFE, decreasing the dynamic contact angle. Fibroblast and epithelial cells were elongated and oriented with their long axis parallel to the fibres, both individually and in clusters. The fibres restricted cell migration. Cell alignment was slightly reduced on the plasma-treated fibres. These results indicated that although surface topography can affect cellular response, surface chemistry also mediates the extent of this response.
authors.affiliation: Clinical Sciences, University of Liverpool, Liverpool, L69 3GA, UK. vkearns@liverpool.ac.uk
authors.forename: V R
authors.initials: VR
authors.lastname: Kearns
authors.forename: P J
authors.initials: PJ
authors.lastname: Doherty
authors.forename: G
authors.initials: G
authors.lastname: Beamson
authors.forename: N
authors.initials: N
authors.lastname: Martin
authors.forename: R L
authors.initials: RL
authors.lastname: Williams
mesh_terms: D002460:Cell Line; D002465:Cell Movement; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D017276:Friction; D005881:Gingiva / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D057139:Nanofibers; D010006:Osteoblasts / Q000378:metabolism*; D011138:Polytetrafluoroethylene / Q000378:metabolism*; D013997:Time Factors
chemical_list: D011138:Polytetrafluoroethylene