title: Development of in situ hybridization for the detection of Mycoplasma hyorhinis in formalin-fixed paraffin-embedded tissues from naturally infected pigs with polyserositis.
abstract: The aim of this study was to develop in situ hybridization for detection of Mycoplasma hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from pigs with polyserositis. M. hyorhinis was isolated from the spleen (2 pigs) and pericardium (1 pig). M. hyorhinis DNA was detected 16 out of 20 pigs with polyserositis. In situ hybridization produced a distinct positive signal for the M. hyorhinis p37 gene in inflammatory cells in the polyserositis. In situ hybridization developed in the present study present diagnostic tools capable of detection of M. hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from the naturally infected pigs.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Gwanak-Gu, Seoul, Republic of Korea.
authors.forename: Bongtae
authors.initials: B
authors.lastname: Kim
authors.forename: Kichan
authors.initials: K
authors.lastname: Lee
authors.forename: Kiwon
authors.initials: K
authors.lastname: Han
authors.forename: Duyeol
authors.initials: D
authors.lastname: Kim
authors.forename: Yooncheol
authors.initials: Y
authors.lastname: Ha
authors.forename: Chung Hyun
authors.initials: CH
authors.lastname: Kim
authors.forename: Yeonsu
authors.initials: Y
authors.lastname: Oh
authors.forename: Ikjae
authors.initials: I
authors.lastname: Kang
authors.forename: Jeehoon
authors.initials: J
authors.lastname: Lee
authors.forename: Chanhee
authors.initials: C
authors.lastname: Chae
mesh_terms: D000818:Animals; D020029:Base Pairing; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification; D006321:Heart / Q000382:microbiology; D017403:In Situ Hybridization / Q000662:veterinary; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D045790:Mycoplasma hyorhinis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012700:Serositis / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial