title: Calcineurin promotes proliferation, migration, and invasion of small cell lung cancer.
abstract: A novel role for calcineurin (Cn) has been reported recently regarding the oncogenic potential in pancreatic and colorectal cancer. The aim of this study was to investigate the putative causal role calcineurin could play in the development of lung cancer with bone metastases. We found that CnAalpha, an isoform of calcineurin, was significantly overexpressed in lung cancer tissues with bone metastasis as compared to tumors with non-bone metastases as investigated by RT-PCR. Strong nuclear staining of tumor cells was observed in small cell lung cancer tissues with bone metastasis. Conversely, cytoplasmic staining of tumor cells was observed in small cell lung cancer tissues with non-bone metastasis. Western blots of nuclear proteins from lung cancer tissues indicated that CnAalpha was highly expressed in lung cancer tissues with bone metastases, but not in those with non-bone metastases. In vitro, it was demonstrated that the CnAalpha gene obviously promoted cell proliferation and inhibited cell apotosis. The CnAalpha gene affected the cell cycle and promoted G1[Symbol: see text]S transition in SBC-3 cells. Transfection with the CnAalpha gene promoted cell migration and invasion. These results indicated that CnAalpha may affect the biological behavior of the human small cell lung cancer cell line SBC-3 in vitro and may be a candidate tumor promotor gene for developing bone metastases.
authors.affiliation: Department of Oncology, Tangdu Hospital, Fourth Military Medical University, No.1 Xinsi Road, Ba Qiao District, Xi'an, 710038, Shaanxi Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Min
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Liu
authors.forename: Ning-Qiang
authors.initials: NQ
authors.lastname: Ma
authors.forename: Ying-Ming
authors.initials: YM
authors.lastname: Feng
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Ping-zhong
authors.initials: PZ
authors.lastname: Wang
authors.forename: De-Dong
authors.initials: DD
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhuang
authors.forename: He-Long
authors.initials: HL
authors.lastname: Zhang
mesh_terms: D001859:Bone Neoplasms / Q000556:secondary; D019703:Calcineurin / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D055752:Small Cell Lung Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D019703:Calcineurin