title: Functional multineuron calcium imaging for systems pharmacology.
abstract: Functional multineuron calcium imaging (fMCI) is a large-scale technique used to access brain function on a single-neuron scale. It detects the activity of individual neurons by imaging action potential-evoked transient calcium influxes into their cell bodies. fMCI has recently been used as a high-throughput research tool to examine how neuronal activity is altered in animal models of brain diseases, for example stroke, Alzheimer's disease, and epilepsy, and to estimate how pharmacological agents act on normal and abnormal states of neuronal networks. It offers unique opportunities to discover the mechanisms underlying neurological disorders and new therapeutic targets.
authors.affiliation: Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Takahashi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takahara
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ishikawa
authors.forename: Norio
authors.initials: N
authors.lastname: Matsuki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ikegaya
mesh_terms: D000200:Action Potentials; D000818:Animals; D001927:Brain Diseases / Q000175:diagnosis* / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D003952:Diagnostic Imaging; D006801:Humans; D009474:Neurons / Q000502:physiology*
chemical_list: D002118:Calcium