title: Involvement of cytoskeleton in orientation of cell division in contact guided cells.
abstract: Single human skin fibroblasts and the skin keratinocyte cell line HaCaT show contact guidance and elongate along narrow (1-2 microm) scratches in glass substratum. During cell division these cells orientate their mitotic spindles along the long axis of the cell. Immunofluorescence staining of actin, tubulin, chromatin, and the nuclear NuMA protein complex demonstrated that cell elongation along scratches is accompanied by a corresponding rearrangement in the cytoskeleton. The results and literature suggest the following steps in the interplay between outside-in and inside-out signalling in the regulation of cell division orientation by extracellular factors. The interaction of cell surface with an anisotropy in the local environment causes changes in F-actin organization, cell elongation and alignment of stress fibres along the cell axis. This is accompanied by a corresponding reorientation of microtubules. Microtubules mediate between cell shape changes dependent upon cell interaction with substratum or other cells, the cortical actin and the position of centrosomes. Centrosomes determine the position and orientation of the mitotic spindle. The astral and central microtubules of the mitotic spindle control the localization of contraction-relaxation in the cell cortex and the position of the constriction ring and cell division plane.
authors.affiliation: Department of Cell Biology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
authors.forename: Gracjana
authors.initials: G
authors.lastname: Krzysiek-Maczka
authors.forename: Marta
authors.initials: M
authors.lastname: Michalik
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Madeja
authors.forename: Wlodzimierz
authors.initials: W
authors.lastname: Korohoda
mesh_terms: D034961:Antigens, Nuclear / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D018797:Cell Cycle Proteins; D002455:Cell Division; D002460:Cell Line; D003599:Cytoskeleton / Q000502:physiology*; D011345:Fenofibrate; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D034521:Nuclear Matrix-Associated Proteins / Q000235:genetics / Q000378:metabolism; D013499:Surface Properties
chemical_list: D034961:Antigens, Nuclear; D018797:Cell Cycle Proteins; C050045:NUMA1 protein, human; D034521:Nuclear Matrix-Associated Proteins; D011345:Fenofibrate