[Impact of rhG-CSF on Sphingosine 1-phosphate receptor 1 expression in CD4+ T cells of donor peripheral blood].
CD4+ T cells mainly interact with Sphingosine 1-phosphate (S1P) to regulate immune function through Sphingosine 1-phosphate receptor 1 (S1P1). This study was aimed to investigate the effects of recombinant human granulocyte-colony-stimulating factor (rhG-CSF) mobilization on S1P1 expression in CD4+ T cells of donor's peripheral blood. The CD4+T cells of peripheral blood were isolated by magnetic beads from 17 allo-hematopoietic stem cell transplantation (allo-HSCT) donors before and at fourth day of mobilization with rhG-CSF. The S1P1 expression was detected by real time quantitative PCR in the RNA extracted from CD4+ T cells collected before and after rhG-CSF mobilization. The results showed that the expression of S1P1 was found in CD4+ cells before and after rhG-CSF mobilization, but the expression level of SIP1 in CD4+ cells after rhG-CSF mobilization was significantly lower than that before rhG-CSF mobilization (p<0.01). It is concluded that the mobilization with rhG-CSF obviously down-regulates the expression of S1P1 in CD4+ T cells of donor's peripheral blood.
Zhu
Hai-Yan
HY
Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
DA
Wan-Ming
WM
Gao
Chun-Ji
CJ
Li
Meng
M
Chen
Wei-Hua
WH
Yü
Li
L
Huang
Wen-Rong
WR
D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D049349:Receptors, Lysosphingolipid / Q000378:metabolism*; D011994:Recombinant Proteins; D000081025:Sphingosine-1-Phosphate Receptors
D049349:Receptors, Lysosphingolipid; D011994:Recombinant Proteins; C477362:S1PR1 protein, human; D000081025:Sphingosine-1-Phosphate Receptors; D016179:Granulocyte Colony-Stimulating Factor