The Scottish Structural Proteomics Facility: targets, methods and outputs.
The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology.
Oke
Muse
M
Biomedical Sciences Research Complex, University of St Andrews, St Andrews, KY16 9ST, UK.
Carter
Lester G
LG
Johnson
Kenneth A
KA
Liu
Huanting
H
McMahon
Stephen A
SA
Yan
Xuan
X
Kerou
Melina
M
Weikart
Nadine D
ND
Kadi
Nadia
N
Sheikh
Md Arif
MA
Schmelz
Stefan
S
Dorward
Mark
M
Zawadzki
Michal
M
Cozens
Christopher
C
Falconer
Helen
H
Powers
Helen
H
Overton
Ian M
IM
van Niekerk
C A Johannes
CA
Peng
Xu
X
Patel
Prakash
P
Garrett
Roger A
RA
Prangishvili
David
D
Botting
Catherine H
CH
Coote
Peter J
PJ
Dryden
David T F
DT
Barton
Geoffrey J
GJ
Schwarz-Linek
Ulrich
U
Challis
Gregory L
GL
Taylor
Garry L
GL
White
Malcolm F
MF
Naismith
James H
JH
D019295:Computational Biology; D003460:Crystallization; D006801:Humans; D007753:Laboratories / Q000458:organization & administration*; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000458:organization & administration*; D012606:Scotland
D011506:Proteins