Non-covalent attachment of proteins to single-walled carbon nanotubes.
A method for the non-covalent attachment of proteins to single-walled carbon nanotubes (SWNTs) is described. In this method, the protein is adsorbed to SWNTs that are suspended using sodium cholate, a surfactant and bile salt. The sodium cholate is then removed by dialysis with retention of the protein on the SWNTs. This method has resulted in good protein loadings and good retention of protein activity.
Neves
Luís F F
LF
School of Chemical, Biological and Materials Engineering, and Carbon Nanotube Technology Center, University of Oklahoma, Norman, OK, USA.
Tsai
Ta-Wei
TW
Palwai
Naveen R
NR
Martyn
David E
DE
Tan
Yongqiang
Y
Schmidtke
David W
DW
Resasco
Daniel E
DE
Harrison
Roger G
RG
D000327:Adsorption; D001647:Bile Acids and Salts / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D020358:Sodium Cholate / Q000737:chemistry; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry
D001647:Bile Acids and Salts; D037742:Nanotubes, Carbon; D011506:Proteins; D013501:Surface-Active Agents; D020358:Sodium Cholate