title: DamIP: a novel method to identify DNA binding sites in vivo.
abstract: Identifying binding sites and target genes of transcription factors is a major biologic problem. The most commonly used current technique, chromatin immunoprecipitation (ChIP), is dependent on a high quality antibody for each protein of interest, which is not always available, and is also cumbersome, involving sequential cross-linking and reversal of cross-linking. We have developed a novel strategy to study protein DNA binding sites in vivo, which we term DamIP. By tethering a mutant form of E. coli DNA adenine methyltransferase to the target protein, the fusion protein introduces N-6-adenosine methylation to sequences proximal to the protein binding sites. DNA fragments with this modification, which is absent in eukaryotes, are detected using an antibody directed against methylated adenosine. For an initial test of the method we used human estrogen receptor alpha (hERalpha), one of the best studied transcription factors. We found that expression of Dam-hERalpha fusion proteins in MCF-7 cells introduces adenosine methylation near a series of known direct hERalpha binding sites. Specific methylation tags are also found at indirect hERalpha binding sites, including both primary binding sites for the ER interactors AP-1 and SP1, and promoters that are activated by upstream ER bound enhancers. DamIP provides a new tool for the study of DNA interacting protein function in vivo.
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Rui
authors.initials: R
authors.lastname: Xiao
authors.forename: Ramon
authors.initials: R
authors.lastname: Roman-Sanchez
authors.forename: David D
authors.initials: DD
authors.lastname: Moore
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics*
chemical_list: D014157:Transcription Factors; D004247:DNA