Evaluation of methicillin resistance in Staphylococcus intermedius isolated from dogs.
Methicillin and multi-drug resistance were investigated in 136 Staphylococcus intermedius strains of canine origin. The large majority of isolates were found to be mecA-negative by polymerase chain reaction, whereas only four strains were positive for the mecA gene. All mecA-positive strains were confirmed as methicillin-resistant by complementary tests, except for oxacillin disk diffusion, which yielded one false-negative result. A significantly higher resistance to fusidic acid, lincosamides, and cotrimoxazole was observed in methicillin-resistant S. intermedius (MRSI) compared with methicillin-susceptible strains. Although the prevalence of MRSI in dogs appeared to be low, methicillin resistance was confirmed to be associated with multi-drug resistance, suggesting the importance of antimicrobial susceptibility testing of canine S. intermedius strains.
Meucci
V
V
Dipartimento di Clinica Veterinaria, Università di Pisa, Pisa, Italy. vmeucci@vet.unipi.it
Vanni
M
M
Guardabassi
L
L
Moodley
A
A
Soldani
G
G
Intorre
L
L
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D016106:Methicillin Resistance; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D057789:Staphylococcus intermedius / Q000187:drug effects* / Q000302:isolation & purification
D000900:Anti-Bacterial Agents