Gingival overgrowth in cyclosporine, tacrolimus, or sirolimus-based immunosuppressive regimens and the single nucleotide IL-6 (-174 G/C) gene polymorphism.
OBJECTIVE
Interleukin-6 (IL-6) may be involved in drug-induced gingival overgrowth (GO). The present study was conducted to assess the association between IL-6 (-174 G/C) gene polymorphism and GO in renal transplant recipients under cyclosporine (CsA), tacrolimus (Tcr), or sirolimus (Sir)-based regimens.


METHODS
Within an eligible population, 45 unrelated subjects were selected for each CsA, Tcr, and Sir group, totaling a sample of 135 subjects. GO was visually assessed and subjects were assigned as controls (non-responders) or cases (responders) in a post hoc definition. IL-6 gene polymorphism was assessed using the polymerase chain reaction amplification and digestion. The distribution of genotypes and allele frequencies in responders and non-responders were compared using the Chi-squared test.


RESULTS
The number of responders was 27 (60.0%), 13 (28.9%), and 7 (15.6%) in the CsA, Tcr, and Sir groups, respectively. No differences could be observed at frequencies of -174GG, -174CG, and -174CC genotypes when comparing responders to non-responders in the CsA, Tcr, and Sir groups. Similar to genotypes, allele frequencies showed no differences between responders and non-responders in all groups.


CONCLUSIONS
No association between IL-6 (-174 G/C) gene polymorphism and gingival overgrowth was observed in renal transplant recipients under CsA, Tcr, or Sir-based immunosuppressive maintenance regimens.
Cota
Luís Otávio Miranda
LO
Department of Dental Clinics, Oral Pathology and Oral Surgery, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Viana
Michelle Beatriz
MB
Moreira
Paula Rocha
PR
Gomez
Ricardo Santiago
RS
Cortelli
José Roberto
JR
Cortelli
Sheila Cavalca
SC
Costa
Fernando Oliveira
FO
D000328:Adult; D002121:Calcium Channel Blockers / Q000627:therapeutic use; D003430:Cross-Sectional Studies; D016572:Cyclosporine / Q000009:adverse effects*; D003596:Cytosine; D003774:Dental Plaque Index; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D019214:Gingival Overgrowth / Q000139:chemically induced* / Q000145:classification / Q000235:genetics; D006147:Guanine; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D015850:Interleukin-6 / Q000235:genetics*; D016030:Kidney Transplantation; D008297:Male; D010512:Periodontal Index; D011110:Polymorphism, Genetic / Q000235:genetics*; D020123:Sirolimus / Q000009:adverse effects*; D016559:Tacrolimus / Q000009:adverse effects*; D013997:Time Factors
D002121:Calcium Channel Blockers; D007166:Immunosuppressive Agents; D015850:Interleukin-6; D006147:Guanine; D016572:Cyclosporine; D003596:Cytosine; D020123:Sirolimus; D016559:Tacrolimus