title: [Effects of Selaginella doederleinii on human nasopharyngeal carcinoma TW03 cells in vitro and its mechanism].
abstract: OBJECTIVE
  To study the inhibition and mechanism of Selaginella doederleinii (SLG) on the growth of nasopharyngeal carcinoma (NPC) TW03 cells in vitro.
  
  
  METHODS
  Cell suppression rates were measured by MTT assay, cell cycles were measured by flow cytometry (FCM) and expressions of Bc1-2 and Bax protein were detected by immunohistochemistry.
  
  
  RESULTS
  Inhibition on the growth of NPC cell was intensed by SLG in a dose and time dependent manner in vitro (P < 0.05). The ratio of S phase cells increased and the ratios of G0/G1 and G2/M phase cells decreased, with the addition of SLG. Meanwhile, the expression of Bcl-2 protein was down-regulated and Bax protein was up-regulated.
  
  
  CONCLUSIONS
  SLG could inhibit the growth of human NPC TW03 cells by directing cell cycle arrested at S phase, down-regulating Bcl-2 protein expression and up-regulating Bax protein expression.
authors.affiliation: First Hospital Affiliated of Guangxi Medical University, Nanning 530021, China. jmywh@126.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Jing
authors.forename: An-zhou
authors.initials: AZ
authors.lastname: Tang
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Kang
authors.forename: Peng
authors.initials: P
authors.lastname: Deng
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D010946:Plants, Medicinal / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D032503:Selaginellaceae / Q000737:chemistry*; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein