title: [Reparative regeneration of rat skin under influence of hollow cathode lamp (HCL) with manganese and copper line spectrum emission].
abstract: Influence of local light exposure by hollow cathode lamp with typical manganese and copper (HCL-Mn, Cu) line emission spectrum on posttraumatic regeneration rate of rat skin has been investigated. We performed the comparative analysis of the morphology and the differentiation ability of rat skin on the 15th and 24th days after full-thickness skin wound had been inflicted on rat dorsums. On the 15th day after injury, the experimental group (daily 30 s exposure for two weeks) showed scab loss, re-epithelialization, and hair regrowth, in contrast to the control rats, where scabs were still observed on the 24th day. Histological analysis revealed that in contrast to the control group the treatment with HCL-Mn, Cu resulted in the increased number of hair follicles and sebaceous glands, the decreased number of blood vessels and horizontal orientation of collagen fibers. The immunohistochemistry for OX-62 revealed that the number of dermal dendritic cells in the experimental groups was maximal on the 15th day, and then decreased to the 24th day after injury. The number of dermal dendritic cells was significantly lower in the control group. The immunohistochemistry for pan-keratins in the control animals revealed a high number of cells expressing different types of keratins, distributed in the main part of the epidermis on the 15th day after surgery, whereas in the experimental group the number of such cells was significantly lower and the cells were concentrated more close to the external part of the epidermis. The number of cells stained for keratin 19 was higher in the experimental group on the 15th day after surgery, whereas this number decreased in this group on the 24th day after surgery as compared to the control group. Thus, typical manganese and copper line spectrum emission emitted by hollow cathode lamp stimulates innate immunity, accelerates restoration of derma, skin epithelium and other skin derivates, and stimulates wound healing in general.
authors.forename: V I
authors.initials: VI
authors.lastname: Mel'nikova
authors.forename: M S
authors.initials: MS
authors.lastname: Izvol'skaia
authors.forename: S N
authors.initials: SN
authors.lastname: Voronova
authors.forename: M M
authors.initials: MM
authors.lastname: Sharipova
authors.forename: E M
authors.initials: EM
authors.lastname: Rukin
authors.forename: L A
authors.initials: LA
authors.lastname: Zakharova
mesh_terms: D000818:Animals; D055984:Cathode Ray Tube; D002455:Cell Division; D003300:Copper / Q000737:chemistry*; D007633:Keratins / Q000378:metabolism; D007801:Langerhans Cells / Q000502:physiology / Q000528:radiation effects; D008297:Male; D008345:Manganese / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012879:Skin Physiological Phenomena / Q000528:radiation effects*; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D008345:Manganese; D007633:Keratins; D003300:Copper