[Establishment of serum peptidome pattern-based diagnostic model of endometriosis].
OBJECTIVE
To establish a diagnostic model of endometriosis by analyzing serum peptidome patterns in patients with endometriosis using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS).


METHODS
Serum samples from 21 endometriosis patients and 29 healthy control subjects were analyzed using MALDI-TOF-MS and Clinprotools 2.0 software to establish the diagnostic model of endometriosis, which was validated using the serum samples from 10 endometriosis patients and 15 healthy controls.


RESULTS
Eighteen statistically significant peptide peaks were obtained with the m/z value ranging from 1,000 to 10,000 (P<0.01). Among the peaks, 12 were down-regulated and 6 up-regulated. The sensitivity and specificity of the model was 90.9% and 100.0%, respectively, with a diagnostic accuracy of 96.2%.


CONCLUSIONS
This model shows the feasibility of using MALDI-TOF-MS and Clinprotools software to identify the biomarkers of endometriosis.
Li
Li-an
LA
General Hospital of PLA, Beijing 100853, China.
Wang
Na
N
Liang
Ting-ting
TT
Li
Ai-ling
AL
Li
Ya-li
YL
D000328:Adult; D015415:Biomarkers / Q000097:blood; D004715:Endometriosis / Q000097:blood / Q000175:diagnosis*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008875:Middle Aged; D008954:Models, Biological; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D055815:Young Adult
D015415:Biomarkers; D020543:Proteome