title: Cramoll 1,4 lectin increases ROS production, calcium levels, and cytokine expression in treated spleen cells of rats.
abstract: This study reports the in vivo stimulatory effects of Cramoll 1,4 on rat spleen lymphocytes as evidenced by an increase in intracellular reactive oxygen species (ROS) production, Ca(2+) levels, and interleukin (IL)-1beta expression. Cramoll 1,4 extracted from seeds of the Leguminosae Cratylia mollis Mart., is a lectin with antitumor and lymphocyte mitogenic activities. Animals (Nine-week-old male albino Wistar rats, Rattus norvegicus) were treated with intraperitoneal injection of Cramoll 1,4 (235 microg ml(-1) single dose) and, 7 days later, spleen lymphocytes were isolated and analyzed for intracellular ROS, cytosolic Ca(2+), and IL-6, IL-10, and IL-1 mRNAs. Cell viability was investigated by annexin V-FITC and 7-amino-actinomycin D staining. The data showed that in lymphocytes activated by Cramoll 1,4 the increase in cytosolic and mitochondrial ROS was related to higher cytosolic Ca(2+) levels. Apoptosis and necrosis were not detected in statistically significant values and thus the lectin effector activities did not induce lymphocyte death. In vivo Cramoll 1,4 treatment led to a significant increase in IL-1beta but IL-6 and -10 levels did not change. Cramoll 1,4 had modulator activities on spleen lymphocytes and stimulated the Th2 response.
authors.affiliation: Departamento de Imunologia, Instituto de Pesquisas Aggeu Magalhães (IAM/FIOCRUZ), Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil. crismout_melo@hotmail.com
authors.forename: Cristiane Moutinho Lagos
authors.initials: CM
authors.lastname: de Melo
authors.forename: Bruno Alves
authors.initials: BA
authors.lastname: Paim
authors.forename: Karina Gotardelo
authors.initials: KG
authors.lastname: Zecchin
authors.forename: Joseani
authors.initials: J
authors.lastname: Morari
authors.forename: Marcos R
authors.initials: MR
authors.lastname: Chiaratti
authors.forename: Maria Tereza Santos
authors.initials: MT
authors.lastname: Correia
authors.forename: Luana Cassandra B
authors.initials: LC
authors.lastname: Barroso Coelho
authors.forename: Patrícia Maria Guedes
authors.initials: PM
authors.lastname: Paiva
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D007274:Injections, Intraperitoneal; D016753:Interleukin-10 / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D037121:Plant Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D037121:Plant Lectins; D017382:Reactive Oxygen Species; D016753:Interleukin-10; D002118:Calcium