title: [Primary studies on chemical constituents from butanol extraction of the thorny elaeagnus leaf and their cytotoxicity].
abstract: OBJECTIVE
  To study the chemical constituents and test the cytotoxicity of the purified compounds from the Thorny Elaeagnus Leaf.
  
  
  METHODS
  The constituents were isolated by column chromatography and their structures were elucidated by chemical properties and spectroscopic analysis. The cytotoxicity of the chemical components for SGC-7901 and Hela cells was measured by MuT method.
  
  
  RESULTS
  Four components were isolated and identified as 3'-O-methylquercetin( 1),kaempferol-3-O-beta-D-6-O-(p-hydroxycinnamoyl) -glucopyranoside(2), kakkalide(3),3'-O-methyl-ellagic acid-4-O-alpha-L-rhamnicoside(4). These components had cytotoxicity for SGC-7901 and Hela cells.
  
  
  CONCLUSIONS
  Compounds 1,3 and 4 are isolated from Thorny Elaeagnus Leaf for the first time. The results of pharmacological experiments indicate that compounds 1 and 2 show strong cytotoxicity to Hela cells.
authors.affiliation: Institute of Materia Medica of Shandong Academy of Medical Sciences, Key Laboratory for Modern Medicine and Technology of Shan-dong Province, Jinan 250062, China.
authors.forename: Yi-cheng
authors.initials: YC
authors.lastname: Fu
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Wang
authors.forename: Xian-hui
authors.initials: XH
authors.lastname: Jia
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D031263:Elaeagnaceae / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D007529:Isoflavones; D044949:Kaempferols; C047368:3-methylquercetin; C108698:kakkalide; D011794:Quercetin