title: S100A10 regulates plasminogen-dependent macrophage invasion.
abstract: The plasminogen activation system plays an integral role in the migration of macrophages in response to an inflammatory stimulus, and the binding of plasminogen to its cell-surface receptor initiates this process. Although previous studies from our laboratory have shown the importance of the plasminogen receptor S100A10 in cancer cell plasmin production, the potential role of this protein in macrophage migration has not been investigated. Using thioglycollate to induce a peritoneal inflammatory response, we demonstrate, for the first time, that compared with wild-type (WT) mice, macrophage migration across the peritoneal membrane into the peritoneal cavity in S100A10-deficient (S100A10(-/-)) mice was decreased by up to 53% at 24, 48, and 72 hours. Furthermore, the number of S100A10-deficient macrophages that infiltrated Matrigel plugs was reduced by 8-fold compared with their WT counterpart in vivo. Compared with WT macrophages, macrophages from S100A10(-/-) mice demonstrated a 50% reduction in plasmin-dependent invasion across a Matrigel barrier and a 45% reduction in plasmin generation in vitro. This loss in plasmin-dependent invasion was in part the result of a decreased generation of plasmin and a decreased activation of pro-MMP-9 by S100A10-deficient macrophages. This study establishes a direct involvement of S100A10 in macrophage recruitment in response to inflammatory stimuli.
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: Paul A
authors.initials: PA
authors.lastname: O'Connell
authors.forename: Alexi P
authors.initials: AP
authors.lastname: Surette
authors.forename: Robert S
authors.initials: RS
authors.lastname: Liwski
authors.forename: Per
authors.initials: P
authors.lastname: Svenningsson
authors.forename: David M
authors.initials: DM
authors.lastname: Waisman
mesh_terms: D000818:Animals; D017306:Annexin A2 / Q000502:physiology*; D017209:Apoptosis; D015153:Blotting, Western; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism; D004338:Drug Combinations; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism; D005434:Flow Cytometry; D007124:Immunoenzyme Techniques; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D007797:Laminin / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010958:Plasminogen / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism; D009418:S100 Proteins / Q000502:physiology*; D013864:Thioglycolates / Q000633:toxicity; D000099248:S100 Calcium Binding Protein A10
chemical_list: D017306:Annexin A2; D003094:Collagen; D004338:Drug Combinations; D005341:Fibrinolysin; D007797:Laminin; D010958:Plasminogen; D011509:Proteoglycans; D009418:S100 Proteins; D013864:Thioglycolates; D000099248:S100 Calcium Binding Protein A10; C059485:matrigel