Minigenome-based reporter system suitable for high-throughput screening of compounds able to inhibit Ebolavirus replication and/or transcription.
We describe an Ebolavirus minigenome-based system that is suitable for high-throughput screening of compounds able to impair Ebolavirus virus replication and/or transcription. The assay is robust (Z' factor, >0.6) and can be carried out in low-biosafety containment. Results from a pilot screen of 960 compounds are presented.
Jasenosky
Luke D
LD
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA.
Neumann
Gabriele
G
Kawaoka
Yoshihiro
Y
D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D029043:Ebolavirus / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D014158:Transcription, Genetic / Q000187:drug effects* / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects* / Q000235:genetics
D000998:Antiviral Agents