Effects of dietary corn oil and vitamin E supplementation on fatty acid profiles and expression of acetyl CoA carboxylase and stearoyl-CoA desaturase gene in Hu sheep.
This study was conducted to assess the effects of dietary corn oil and vitamin E supplementation on fatty acid (FA) profiles and abundances of acetyl-CoA carboxylase (ACC) and Delta(9) stearoyl-CoA desaturase (SCD) mRNA of Hu sheep. Animals were allocated to three dietary treatments: basal and supplemented with 3% corn oil (CNO), or CNO plus 500 mg/kg vitamin E (COE). The experiment lasted for 10 weeks. No differences were observed in growth performance and carcass qualities among the three treatments (P > 0.05). Feeding CNO and COE diets increased polyunsaturated FAs including cis 9 trans 11 conjugated linoleic acid, and decreased saturated FA in longissimus muscle (P < 0.05). The mRNA abundances of ACC and SCD as detected by real-time PCR were reduced (P < 0.05) in liver and subcutaneous fat by supplementary oil, while the SCD mRNA level in longissimus muscle was also reduced (P < 0.05). Inclusion of vitamin E did not have further effects on mRNA abundances of these two enzymes. It is suggested that dietary corn oil supplementation may reduce FA biosynthesis and influence FA profiles in Hu sheep through decreased expression of both ACC and SCD genes.
Chen
Xue-jun
XJ
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
Mao
Hui-lin
HL
Ma
Xiao-mei
XM
Liu
Jian-xin
JX
D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000818:Animals; D003314:Corn Oil / Q000008:administration & dosage*; D019587:Dietary Supplements; D005227:Fatty Acids / Q000096:biosynthesis*; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012756:Sheep / Q000235:genetics* / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics*; D014810:Vitamin E / Q000008:administration & dosage*
D005227:Fatty Acids; D012333:RNA, Messenger; D014810:Vitamin E; D003314:Corn Oil; D013230:Stearoyl-CoA Desaturase; D000103:Acetyl-CoA Carboxylase