title: An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy.
abstract: Interest in developing robust, quicker and easier diagnostic tests for cancer has lead to an increased use of Fourier transform infrared (FTIR) spectroscopy to meet that need. In this study we present the use of different experimental modes of infrared spectroscopy to investigate the RWPE human prostate epithelial cell line family which are derived from the same source but differ in their mode of transformation and their mode of invasive phenotype. Importantly, analysis of the infrared spectra obtained using different experimental modes of infrared spectroscopy produces similar results. The RWPE family of cell lines can be separated into groups based upon the method of cell transformation rather than the resulting invasiveness/aggressiveness of the cell line. The study also demonstrates the possibility of using a genetic algorithm as a possible standardised pre-processing step and raises the important question of the usefulness of cell lines to create a biochemical model of prostate cancer progression.
authors.affiliation: Manchester Interdisciplinary Biocentre, Centre for Instrumentation and Analytical Science, School of Chemical Engineering and Analytical Science, The University of Manchester, 131 Princess Street, Manchester, UK M1 7DN.
authors.forename: M J
authors.initials: MJ
authors.lastname: Baker
authors.forename: C
authors.initials: C
authors.lastname: Clarke
authors.forename: D
authors.initials: D
authors.lastname: Démoulin
authors.forename: J M
authors.initials: JM
authors.lastname: Nicholson
authors.forename: F M
authors.initials: FM
authors.lastname: Lyng
authors.forename: H J
authors.initials: HJ
authors.lastname: Byrne
authors.forename: C A
authors.initials: CA
authors.lastname: Hart
authors.forename: M D
authors.initials: MD
authors.lastname: Brown
authors.forename: N W
authors.initials: NW
authors.lastname: Clarke
authors.forename: P
authors.initials: P
authors.lastname: Gardner
mesh_terms: D000465:Algorithms; D002461:Cell Line, Transformed; D016002:Discriminant Analysis; D004847:Epithelial Cells / Q000166:cytology; D005819:Genetic Markers; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D025341:Principal Component Analysis; D011467:Prostate / Q000166:cytology; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D005819:Genetic Markers