Insight in the transport behavior of copper glycinate complexes through the porcine gastrointestinal membrane using an Ussing chamber assisted by mass spectrometry analysis.
An Ussing chamber study was conducted in order to investigate the transport behavior of copper glycinate complexes through a porcine gastrointestinal membrane. Organic copper complexes such as copper tri- and tetraglycinates (GGG-Cu(II) and GGGG-Cu(II)) were used as model system. In a novel analytical approach the Ussing chamber was combined with mass spectrometry. Therefore, relevant analytical methods based on MALDI-MS and a coupling of capillary electrophoresis to ICP-MS and ESI-MS were developed for the determination of copper complexes in the mucosal and serosal half-chambers. It was found that 86.1+/-8.5% of copper triglycinate but only 20.8+/-9.9% of copper tetraglycinate penetrated the digestive membrane without modification. Furthermore, inorganic copper species were not detected but a new copper complex (m/z 442) was found to be formed in both compartments of the Ussing chamber.
Tastet
Laure
L
CNRS/Université de Pau, Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, UMR 5254, Hélioparc, 2 Av du Président Angot, F-64053 Pau, France.
Schaumlöffel
Dirk
D
Yiannikouris
Alexandros
A
Power
Ronan
R
Lobinski
Ryszard
R
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods; D041981:Gastrointestinal Tract / Q000033:anatomy & histology / Q000378:metabolism; D005998:Glycine / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D009942:Organometallic Compounds / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D034421:Sus scrofa
D009942:Organometallic Compounds; C018493:cupric glycinate; D005998:Glycine