Heterogeneity of Leishmania infantum chagasi kinetoplast DNA in Teresina (Brazil).
Leishmania infantum chagasi is the causative agent of visceral leishmaniasis in Brazil. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of kinetoplast DNA (kDNA) minicircles was used to evaluate genetic profiles of 48 Leishmania infantum chagasi strains from dog and human parasite cultures, fresh collected dog bone marrow aspirates, and from infected sand flies. Results revealed that heterogeneity in kDNA minicircles depends mostly on the source of the samples, with cultured parasites showing a high degree of homogeneity.
Alonso
Diego Peres
DP
Department of Parasitology, Bioscience Institute, Sao Paulo State University, Botucatu, Brazil (UNESP).
Costa
Dorcas Lamounier
DL
de Mendonça
Ivete Lopes
IL
Costa
Carlos Henrique Nery
CH
Ribolla
Paulo Eduardo Martins
PE
D000818:Animals; D001938:Brazil / Q000453:epidemiology; D018105:DNA, Kinetoplast / Q000235:genetics*; D004285:Dogs; D014644:Genetic Variation; D006801:Humans; D018314:Leishmania infantum / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000453:epidemiology / Q000469:parasitology*; D010802:Phylogeny
D018105:DNA, Kinetoplast