title: [Preparation of monoclonal antibodies against HN protein of Class I Newcastle disease virus virulent strain].
abstract: OBJECTIVE
  To prepare monoclonal antibodies (mAb) against HN protein of Class I Newcaslte disease virus.
  
  
  METHODS
  Several 6-week-old mice were immunized with ClassI 9a5b virulent strain. Hemagglutination inhibition (HI), indirect immunofluorescence assay (IFA), cell neutralization test, ELISA and Western blot analysis were used to characterize and identify these antibodies.
  
  
  RESULTS
  Four hybridoma cell lines were successfully prepared, designated as mAb 2F5, 2F12, 3H7 and 3H9. Among them, only mAb 3H7 and 3H9 recognized HN protein of the ClassI strain, specifically neutralizing NDV and inhibiting hemagglutination.
  
  
  CONCLUSIONS
  mAb 3H7 and 3H9 could be of use in identification of Class I and Class II strains, and in functional studies of HN and cell receptors for NDV.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
authors.forename: Ying-jie
authors.initials: YJ
authors.lastname: Sun
authors.forename: Hong-jun
authors.initials: HJ
authors.lastname: Chen
authors.forename: Cui-ping
authors.initials: CP
authors.lastname: Song
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Xu-sheng
authors.initials: XS
authors.lastname: Qiu
authors.forename: Sheng-qing
authors.initials: SQ
authors.lastname: Yu
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015707:HN Protein / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D009522:Newcastle disease virus / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*
chemical_list: D000911:Antibodies, Monoclonal; D015707:HN Protein