title: [Polymorphism in exon 2 of pig FIT1 gene and its association with fat-deposition-related traits].
abstract: In pig industry, fat deposition related traits such as back fat thickness and fat rate are of great economic importance. Thus, research on genes related with fat deposition can offer many useful values theoretically and practically. Gene FIT1 (Fat-inducing transcript 1) plays an important role in packaging lipid droplets. Here, we used FIT1 gene as the candidate gene for fat deposition. Sequence comparison revealed that an insertion/deletion mutation occurred at 590~595 bp of the second exon. We then carried out PCR-SSCP analysis followed by association analysis in F2 "Large white xMeishan" resource family. In all the individuals tested, all Meishan pigs possessed the insertion, which was designated allele A, while most Large white pigs possessed the deletion and was named as allele B. Association analysis in F2 resource family showed that this site was highly associated with fat percentage (FP), 6-7 rib fat thickness (RFT), buttock fat thickness (BFT), leaf fat weigh (LFW), total internal fat weigh (TFW), and internal fat rate (IFR) (Plt;0.01). These results indicated that FIT1 gene may have some important values for application. Further and deep research is necessary for revealing more information on this gene in order to provide a new marker for molecular marker-assisted selection breeding.
authors.affiliation: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. lidezhen85@163.com
authors.forename: De-Zhen
authors.initials: DZ
authors.lastname: Li
authors.forename: Jun-Xian
authors.initials: JX
authors.lastname: He
authors.forename: Ming-Gang
authors.initials: MG
authors.lastname: Lei
authors.forename: De-Quan
authors.initials: DQ
authors.lastname: Xu
authors.forename: Si-Wen
authors.initials: SW
authors.lastname: Jiang
authors.forename: Yuan-Zhu
authors.initials: YZ
authors.lastname: Xiong
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D005091:Exons / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D013552:Swine / Q000033:anatomy & histology* / Q000235:genetics* / Q000378:metabolism
chemical_list: D008565:Membrane Proteins