Antioxidant properties of Baccharis trimera in the neutrophils of Fisher rats.
BACKGROUND
Baccharis trimera (Less.) (Asteraceae) is a native plant of Brazil. Also known as "carqueja", it has been popularly used to treat liver diseases, diabetes, as well as digestive disorders. Other studies have described the hepatoprotective, antioxidant and anti-inflammatory activities of the species.


OBJECTIVE
The aim of the present study was to investigate the antioxidant properties of Baccharis trimera in the neutrophils of Fisher rats in both in vitro and in vivo experimental models.


METHODS
In the in vitro assay, the neutrophils of male rats were isolated and incubated with Baccharis trimera extract at concentrations of 0.5, 5.0 and 50.0 microg/mL. In the in vivo assay, male rats were first treated with crude extract 600 mg/kg body weight of Baccharis trimera or with 50 mg/kg body weight of quercetin (reference substance) and then treated with 835 mg/kg of acetaminophen (APAP) after 24 h.


RESULTS
The hydroethanolic extract of Baccharis trimera reduced the release of reactive oxygen species in the neutrophils in both the in vitro and in vivo experimental models. Therefore confirming its antioxidant effect.


CONCLUSIONS
The results of this study confirm the antioxidant effect of Baccharis trimera.
Pádua
Bruno da Cruz
Bda C
Programa de Pós-graduação em Ciências Biológicas do Núcleo de Pesquisas em Ciências Biológicas-NUPEB, Universidade Federal de Ouro Preto (UFOP), Ouro Preto, MG, Brazil.
Silva
Lucas Dornela
LD
Rossoni Júnior
Joamyr Victor
JV
Humberto
Jorge Luiz
JL
Chaves
Míriam Martins
MM
Silva
Marcelo Eustáquio
ME
Pedrosa
Maria Lucia
ML
Costa
Daniela Caldeira
DC
D000082:Acetaminophen / Q000494:pharmacology; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D031175:Baccharis / Q000737:chemistry*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013997:Time Factors; D015054:Zymosan / Q000494:pharmacology
D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D000082:Acetaminophen; D015054:Zymosan; D011794:Quercetin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase