Detection of Jeotgalicoccus spp. in poultry house air.
Investigations of bioaerosols collected from turkey, chicken and duck houses, as well as from a duck slaughterhouse, each in triplicate, revealed that 4-18% of 16S rRNA gene sequences in investigated 16S rRNA gene clone libraries were closely related to Jeotgalicoccus spp. J. halotolerans- and J. psychrophilus-related sequences were obtained in all investigated bioaerosol samples and formed a distinct group with sequences of both species type strains, which were collectively entitled Jeot-cluster-I. For a quantification of Jeot-cluster-I bacteria, a group specific PCR primer combination targeting the 16S rRNA genes was developed. Estimated concentrations by quantitative real-time PCR analyses revealed cell numbers between 10(4) and 10(6)Jeotgalicoccus cellsm(-3) air in turkey, duck, and chicken houses, respectively. These results indicated the remarkable proportion (1-39%) of total cell counts and the hitherto unknown wide distribution of Jeotgalicoccus spp. in the poultry rearing industry.
Martin
Elena
E
Bundesanstalt für Arbeitsschutz und Arbeitsmedizin, Nöldnerstrasse 40-42, 10317 Berlin, Germany.
Fallschissel
Kerstin
K
Kämpfer
Peter
P
Jäckel
Udo
U
D000336:Aerosols; D000391:Air Microbiology; D000818:Animals; D002645:Chickens; D016000:Cluster Analysis; D015169:Colony Count, Microbial / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004372:Ducks; D006799:Housing, Animal; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D040141:Staphylococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014422:Turkeys
D000336:Aerosols; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S