SWIFT detection of SPIO-labeled stem cells grafted in the myocardium.
We report initial results from studies using sweep imaging with Fourier transformation (SWIFT) to detect superparamagnetic iron oxide (SPIO) particle-labeled stem cells in the rat heart. In experiments performed on phantoms containing titanium balls or SPIO-labeled cells, frequency-shifted signals surrounding the paramagnetic objects produced a pileup artifact visualized by SWIFT. Total signal intensity was retained to a much greater extent by SWIFT as compared to gradient echo imaging. SWIFT imaging of excised and in vivo hearts showed (a) reduced blooming artifact as compared with gradient echo imaging, which helped reduce ambiguity in the detection of SPIO-labeled cells; (b) enhancement of off-resonance signals relative to the background in the imaginary component of images; and (c) detailed myocardial anatomy in magnitude images, which provided anatomic reference. These features suggest SWIFT can facilitate the detection of SPIO-laden cells in the cardiovascular system.
Zhou
Rong
R
Laboratories of Molecular Imaging, Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Idiyatullin
Djaudat
D
Moeller
Steen
S
Corum
Curt
C
Zhang
Hualei
H
Qiao
Hui
H
Zhong
Jia
J
Garwood
Michael
M
D000818:Animals; D006348:Cardiac Surgical Procedures; D002478:Cells, Cultured; D003911:Dextrans; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000637:transplantation*; D005260:Female; D052203:Ferrosoferric Oxide; D007089:Image Enhancement / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods*; D058185:Magnetite Nanoparticles; D051379:Mice; D008819:Mice, Nude; D009206:Myocardium / Q000166:cytology*; D019047:Phantoms, Imaging; D051381:Rats; D011923:Rats, Nude; D013194:Staining and Labeling / Q000379:methods
D003911:Dextrans; D058185:Magnetite Nanoparticles; C097921:ferumoxtran-10; D052203:Ferrosoferric Oxide