title: T-lymphoblastic lymphoma with an unusual t(8;14)(q24;q11)--case report.
abstract: Cytogenetic abnormalities seen at presentation of acute lymphoblastic leukemia or lymphoblastic lymphoma (ALL/ LBL) are associated with distinct clinical and hematologic disease entities. T-ALL/LBL are morphologically indistinguishable from those of B-ALL/LBL. An abnormal kariotype is found in 50-70% of cases of T-ALL/LBL. We present a 35-year old male patient with T-ALL/LBL and t(8;14)(q24;q11.2). Our patient presented with B-symptoms, bulky mediastinal disease and CNS infiltration. Bone marrow was morphologically normal and cytogenetically without clonal aberrations. Cytological findings of the supraclavicular lymph node showed numerous CD3 positive (100%) and CD2 positive (88%) lymphoblasts, negative for CD34 and CD10. Flow cytometry of lymph node revealed T cell phenotype of immature cells: CD45+CD2+CD5+CD7+CD4+CD8+CD3cyt +CD3TdT+CD10-CD34-HLAD/DR-. Cytogenetic analysis of lymph node showed translocation t(1;4)(p32;p12), t(8;14)(q24;q11.2). Southern blot analysis of extracted DNA from the supraclavicular lymph node demonstrated clonal rearrangement of the T cell antigen receptor (TCR/J) gene (region Vb+Jb2). Based on these findings, diagnosis of T lymphoblastic non Hodgkin lymphoma was established. Cerebrospinal fluid analysis showed CNS infiltration with 49% lymphoblasts positive for CD4 and CD8. The disease progressed rapidly with poor response to therapy. T-ALL/LBL with an unusual t(8;14)(q24;q11.2) is a very rare hematologic disorder with rapid disease progression and poor response to conventional therapy because of frequent central nervous system involvement and early relapses.
authors.affiliation: University Hospital "Merkur", Zagreb, Croatia. imandac@yahoo.com
authors.forename: Inga
authors.initials: I
authors.lastname: Mandac
authors.forename: Slobodanka Ostojić
authors.initials: SO
authors.lastname: Kolonić
authors.forename: Radovan
authors.initials: R
authors.lastname: Vrhovac
authors.forename: Ruzica
authors.initials: R
authors.lastname: Lasan-Trcić
authors.forename: Jasminka
authors.initials: J
authors.lastname: Jakelić-Pitesa
authors.forename: Ika
authors.initials: I
authors.lastname: Kardum-Skelin
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations; D002883:Chromosomes, Human, Pair 14; D002898:Chromosomes, Human, Pair 8; D017809:Fatal Outcome; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008198:Lymph Nodes / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000473:pathology*; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology*; D013601:T-Lymphocytes / Q000473:pathology; D014178:Translocation, Genetic