title: Mycoplasma mucosicanis sp. nov., isolated from the mucosa of dogs.
abstract: Fourteen Mycoplasma strains were isolated from the oral cavity and genital tract of asymptomatic dogs. Isolates had been preliminarily identified by conventional serological testing as Mycoplasma bovigenitalium, but in 16S-23S rRNA intergenic spacer PCR-RFLP assays the isolates exhibited an RFLP pattern distinct from M. bovigenitalium PG11(T). Analysis of the 16S rRNA gene placed a representative of the isolates (strain 1642(T)) in the M. bovigenitalium subcluster of the Mycoplasma bovis cluster of mycoplasmas, with the highest sequence similarities to Mycoplasma californicum ST-6(T) (96.4 %), M. bovigenitalium PG11(T) (96.3 %) and Mycoplasma phocirhinis 852(T) (96.2 %). 16S rRNA gene sequence similarities almost equidistant from three recognized species and results obtained by sequence analysis of the 16S-23S rRNA intergenic spacer region, polar lipid profiles and serological reactions indicated that this organism represents a novel species of the genus Mycoplasma for which the name Mycoplasma mucosicanis sp. nov. is proposed, with strain 1642(T) ( = ATCC BAA-1895(T)  = DSM 22457(T)) as the type strain.
authors.affiliation: Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
authors.forename: Joachim
authors.initials: J
authors.lastname: Spergser
authors.affiliation: Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Langer
authors.affiliation: Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
authors.forename: Simone
authors.initials: S
authors.lastname: Muck
authors.affiliation: Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Macher
authors.affiliation: Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Szostak
authors.affiliation: Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
authors.forename: Renate
authors.initials: R
authors.lastname: Rosengarten
authors.affiliation: Institute of Microbiology and Genetics, University of Vienna, A-1030 Vienna, Austria.|Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer; D004285:Dogs; D005835:Genitalia / Q000382:microbiology; D008969:Molecular Sequence Data; D058889:Molecular Typing; D009055:Mouth / Q000382:microbiology; D009092:Mucous Membrane / Q000382:microbiology*; D009174:Mycoplasma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S