title: Panacagrimonas perspica gen. nov., sp. nov., a novel member of Gammaproteobacteria isolated from soil of a ginseng field.
abstract: A taxonomic study was carried out on Gsoil 142(T), a bacterial strain isolated from the soil collected in a ginseng field in Pocheon province, South Korea. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this bacterium to the Gammaproteobacteria, and it was most closely related to Hydrocarboniphaga effusa ATCC BAA 332(T) (94.4%, 16S rRNA gene sequence similarity), Nevskia ramosa DSM 11499(T) (94.1%) and Alkanibacter difficilis MN154.3(T) (92.0%). Strain Gsoil 142(T) was a gram-negative, strictly aerobic, motile, and rod-shaped bacterium. The G+C content of the genomic DNA was 69.9% and predominant ubiquinone was Q-8. Major fatty acids were summed feature 8 (C(18:1) omega7c and/or omega6c, 36.3%), summed feature 3 (iso-C(15:0) 2-OH and/or C(16:1) omega7c, 20.6%) and C(16:0) (17.4%). The major polar lipids detected in strain Gsoil 142(T) were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and an unknown glycolipid. On the basis of polyphasic evidence, it is proposed that strain Gsoil 142(T) should be placed in a novel genus and species, for which the name Panacagrimonas perspica gen. nov., sp. nov. is proposed. The type strain is Gsoil 142(T) (= KCTC 12982(T) = LMG 23239(T)).
authors.affiliation: Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Wan-Taek
authors.initials: WT
authors.lastname: Im
authors.forename: Qing-Mei
authors.initials: QM
authors.lastname: Liu
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Yang
authors.forename: Min-Seok
authors.initials: MS
authors.lastname: Kim
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Sung-Taik
authors.initials: ST
authors.lastname: Lee
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D005894:Panax / Q000254:growth & development*; D010743:Phospholipids / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000032:analysis; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone