title: Organic electrochemical transistor based immunosensor for prostate specific antigen (PSA) detection using gold nanoparticles for signal amplification.
abstract: We demonstrated a highly sensitive organic electrochemical transistor (OECT) based immunosensor with a low detection limit for prostate specific antigen/alpha1-antichymotrypsin (PSA-ACT) complex. The poly(styrenesulfonate) doped poly(3,4-ethylenedioxythiophene) (PEDOT:PSS) based OECT with secondary antibody conjugated gold nanoparticles (AuNPs) provided a detection limit of the PSA-ACT complex as low as 1pg/ml, as well as improved sensitivity and a dynamic range, due to the role of AuNPs in the signal amplification. The sensor performances were particularly improved in the lower concentration range where the detection is clinically important for the preoperative diagnosis and screening of prostate cancer. This result shows that the OECT-based immunosensor can be used as a transducer platform acceptable to the point-of-care (POC) diagnostic systems and demonstrates adaptability of organic electronics to clinical applications.
authors.affiliation: Department of Advanced Materials Science & Engineering, Sungkyunkwan University, 300 Chunchun-dong, Jangan-gu, Suwon-si, Gyeonggi-do 440-746, South Korea.
authors.forename: Duck-Jin
authors.initials: DJ
authors.lastname: Kim
authors.forename: Nae-Eung
authors.initials: NE
authors.lastname: Lee
authors.forename: Joon-Shik
authors.initials: JS
authors.lastname: Park
authors.forename: In-Jun
authors.initials: IJ
authors.lastname: Park
authors.forename: Jung-Gu
authors.initials: JG
authors.lastname: Kim
authors.forename: Hyoung J
authors.initials: HJ
authors.lastname: Cho
mesh_terms: D000669:Amplifiers, Electronic; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009930:Organic Chemicals / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014173:Transistors, Electronic
chemical_list: D009930:Organic Chemicals; D006046:Gold; D017430:Prostate-Specific Antigen