Analysis of genetic diversity in banana cultivars (Musa cvs.) from the South of Oman using AFLP markers and classification by phylogenetic, hierarchical clustering and principal component analyses.
Banana is an important crop grown in Oman and there is a dearth of information on its genetic diversity to assist in crop breeding and improvement programs. This study employed amplified fragment length polymorphism (AFLP) to investigate the genetic variation in local banana cultivars from the southern region of Oman. Using 12 primer combinations, a total of 1094 bands were scored, of which 1012 were polymorphic. Eighty-two unique markers were identified, which revealed the distinct separation of the seven cultivars. The results obtained show that AFLP can be used to differentiate the banana cultivars. Further classification by phylogenetic, hierarchical clustering and principal component analyses showed significant differences between the clusters found with molecular markers and those clusters created by previous studies using morphological analysis. Based on the analytical results, a consensus dendrogram of the banana cultivars is presented.
Opara
Umezuruike Linus
UL
Department of Horticultural Science, Faculty of AgricSciences, University of Stellenbosch, Stellenbosch 7602, South Africa. opara@sun.ac.za
Jacobson
Dan
D
Al-Saady
Nadiya Abubakar
NA
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D002874:Chromosome Mapping / Q000379:methods; D016000:Cluster Analysis; D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics*; D028521:Musa / Q000145:classification* / Q000235:genetics*; D009850:Oman; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D025341:Principal Component Analysis
D005819:Genetic Markers