Cloning and functional analysis of the peanut iron transporter AhIRT1 during iron deficiency stress and intercropping with maize.
In previous research, iron-deficiency symptoms in peanut (Arachis hypgaea) were alleviated during anthesis by intercropping with maize. This benefit was associated with increased phytosiderophore secretion by maize and increased Fe(III)-chelate reductase activity by peanut. In the present study, we isolated the full-length cDNA of AhIRT1 (iron-regulated transporter 1) from peanut and characterized how iron deficiency and intercropping affected its iron-transporting ability. Functional complementation with AhIRT1 restored normal growth of the yeast mutant fet3fet4 (defective in both high- and low-affinity iron-uptake systems) under iron-deficiency conditions. Based on transient expression analysis, AhIRT1 was determined to be a membrane protein, which was consistent with a function in iron uptake. In peanut, transcript levels of AhIRT1 increased in both root and shoot under iron-deficiency conditions. In a pot experiment, AhIRT1 transcript levels in intercropped peanut were 10 times greater during anthesis than pre-anthesis, and transcript levels during anthesis were 40% greater in intercropped than in monocropped peanut.
Ding
Hong
H
Key Laboratory of Plant and Soil Interactions, Ministry of Education, College of Resources and Environmental Sciences, China Agricultural University, Beijing, China.
Duan
Lihong
L
Li
Jing
J
Yan
Huifeng
H
Zhao
Meng
M
Zhang
Fusuo
F
Li
Wen-Xue
WX
D000383:Agriculture / Q000379:methods*; D000595:Amino Acid Sequence; D010367:Arachis / Q000235:genetics*; D001692:Biological Transport; D018533:Biomass; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D000090463:Iron Deficiencies; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological; D003313:Zea mays / Q000254:growth & development*
D018744:DNA, Plant; D008565:Membrane Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins