[Immunosuppressive triterpenes from Tetraena mongolica].
OBJECTIVE
To study the immunosuppressive constituents from Tetraena mongolica.


METHODS
Chemical constituents were isolated and purified by repeated column chromatography( silica gel, Toyopearl HW40C and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The MTT assay was applied to evaluate the isolated compounds on the inhibition effect of lymphocyte transformation.


RESULTS
Six triterpenes were isolated and their structures were identified as follows: 3beta-hydroxy-11alpha, 12alpha:13beta,28-diepoxyoleanane(1), 3beta-(3, 4-dihydroxycinnamoyl)-erythrodi-ol(2), olean-28-al-3beta-yl-caffeate(3), erythrodiol (4), 12-oleanaen-3beta-caffeate(5), 3-O-(E) -coumaroylerythrodiol(6). Compound 24 exhibited the inhibition effects on lymphocyte transformation.


CONCLUSIONS
Compounds 1-6 were isolated from this plant for the first time, and compound 1 was a new nature product. Compound 2-4 showed significant immunosuppressive activity.
Ding
Linlin
L
Research Center of Basic Medical Sciences, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China.
Liu
Qiang
Q
Hu
Jiaxu
J
Tang
Shengan
S
Duan
Hongquan
H
D000818:Animals; D002478:Cells, Cultured; D007166:Immunosuppressive Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D028461:Zygophyllaceae / Q000737:chemistry*
D007166:Immunosuppressive Agents; D014315:Triterpenes