title: Mucositis does not lead to false-positivity of the Platelia Aspergillus enzyme-linked immunosorbent assay.
abstract: We are reporting a study evaluating the crossover of antigens reacting in Platelia Aspergillus (PA) enzyme-linked immunosorbent assay (ELISA) from faeces to vessels during mucositis as a possible cause of false-positivity of this test. In our series of 102 episodes of different grades of mucositis, we found strong reactivity of faeces in the PA ELISA test irrespective of the grade of mucositis, the percentage of oral food intake or the presence of total parenteral nutrition. However, none of the patients included in the study were positive in the serum (when the criterion of two samples with cut-off index of positivity [IP] > 0.5 was used).
authors.affiliation: Department of Internal Medicine Hemato-Oncology, Masaryk University and University Hospital Brno, Jihlavska 20, 625 00 Brno, Czech Republic. zracil@fnbrno.cz
authors.forename: Z
authors.initials: Z
authors.lastname: Racil
authors.forename: I
authors.initials: I
authors.lastname: Kocmanova
authors.forename: B
authors.initials: B
authors.lastname: Weinbergerova
authors.forename: M
authors.initials: M
authors.lastname: Toskova
authors.forename: J
authors.initials: J
authors.lastname: Winterova
authors.forename: M
authors.initials: M
authors.lastname: Lengerova
authors.forename: L
authors.initials: L
authors.lastname: Buresova
authors.forename: S
authors.initials: S
authors.lastname: Timilsina
authors.forename: J
authors.initials: J
authors.lastname: Mayer
mesh_terms: D000328:Adult; D000368:Aged; D001228:Aspergillosis / Q000175:diagnosis*; D001230:Aspergillus / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005189:False Positive Reactions; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D052016:Mucositis / Q000150:complications*; D012680:Sensitivity and Specificity