title: Recent insights in pre-mRNA 3'-end processing signals and proteins in the protozoan parasite Entamoeba histolytica.
abstract: The messenger RNA precursors (pre-mRNA) 3'-end processing occurs in a two-step co-transcriptional coupled reaction, denoted as cleavage and polyadenylation. Both processes depend on trans-acting factors interacting in a coordinated manner with cis-sequence motifs located at the 3' untranslated region of transcripts. In this paper, we reviewed mechanisms involved in pre-mRNA processing in eukaryotic organisms, including our own findings about sequences and proteins potentially involved in mRNA 3'-end formation in the protozoan parasite Entamoeba histolytica. Interestingly, protein sequence comparisons among E. histolytica, yeast, and human pre-mRNA processing machineries showed that amoeba pre-mRNA 3'-end processing machinery appears to be in an intermediate evolutionary position between mammals and yeast. In addition, the presence of non canonical poly(A) polymerases family recently identified in E. histolytica, adds more complexity to the mRNA 3'-end formation process in this ancient eukaryote.
authors.affiliation: Universidad Autónoma de la Ciudad de México, Posgrado en Ciencias Genómicas, México D.F., México. genomicas@yahoo.com.mx
authors.forename: César
authors.initials: C
authors.lastname: López-Camarillo
authors.forename: Olga N
authors.initials: ON
authors.lastname: Hernández de la Cruz
authors.forename: Jessica García
authors.initials: JG
authors.lastname: Vivas
authors.forename: Jorge Fernández
authors.initials: JF
authors.lastname: Retana
authors.forename: Marisol Pezet
authors.initials: MP
authors.lastname: Valdez
authors.forename: Itzel Lopez
authors.initials: IL
authors.lastname: Rosas
authors.forename: Elizbeth
authors.initials: E
authors.lastname: Alvarez-Sánchez
authors.forename: Laurence A
authors.initials: LA
authors.lastname: Marchat
mesh_terms: D020413:3' Untranslated Regions; D004748:Entamoeba histolytica / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity; D004749:Entamoebiasis / Q000469:parasitology; D018503:Genome, Protozoan; D006801:Humans; D008954:Models, Biological; D011062:Polynucleotide Adenylyltransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D016053:RNA, Protozoan / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D039221:mRNA Cleavage and Polyadenylation Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D015800:Protozoan Proteins; D012322:RNA Precursors; D016053:RNA, Protozoan; D039221:mRNA Cleavage and Polyadenylation Factors; D011062:Polynucleotide Adenylyltransferase