title: The pericyte and stromal cell marker CD248 (endosialin) is required for efficient lymph node expansion.
abstract: CD248 is a cell surface receptor that specifically identifies fibroblasts and pericytes during development and in association with cancer and inflammation. However, its function is poorly defined and its role in lymphoid organs not studied. Here, we used (4-hydroxy-3-nitrophenyl)acetyl chicken gamma-globulin immunisation and mice lacking CD248 to study whether CD248 modulates popliteal LN (pLN) expansion and subsequent immune responses. We have found that CD248 is required for complete pLN expansion but not for co-ordination of B and T cell compartmentalisation or antibody production following (4-hydroxy-3-nitrophenyl)acetyl chicken gamma-globulin immunisation. In vitro, we show that CD248 expression in human MG63 stromal cells and mouse embryonic fibroblasts leads to a pro-proliferative and pro-migratory phenotype. This correlates with a proliferating CD248(+) population observed in vivo during pLN expansion. Taken together, these data highlight a role for CD248 in secondary lymphoid organ remodelling during adaptive immune responses.
authors.affiliation: Rheumatology Research Group, School of Immunity and Infection, MRC Centre for Immune Regulation, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, UK.
authors.forename: Siân
authors.initials: S
authors.lastname: Lax
authors.forename: Debbie L
authors.initials: DL
authors.lastname: Hardie
authors.forename: Amy
authors.initials: A
authors.lastname: Wilson
authors.forename: Mike R
authors.initials: MR
authors.lastname: Douglas
authors.forename: Graham
authors.initials: G
authors.lastname: Anderson
authors.forename: David
authors.initials: D
authors.lastname: Huso
authors.forename: Clare M
authors.initials: CM
authors.lastname: Isacke
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Buckley
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000235:genetics; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000276:immunology / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology; D015415:Biomarkers / Q000378:metabolism; D002450:Cell Communication / Q000235:genetics; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002645:Chickens; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D006241:Haptens / Q000276:immunology; D006801:Humans; D007114:Immunization; D008198:Lymph Nodes / Q000254:growth & development / Q000276:immunology* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D009596:Nitrophenols / Q000276:immunology; D020286:Pericytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D010648:Phenylacetates / Q000276:immunology; D017154:Stromal Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; D015415:Biomarkers; C078203:CD248 protein, human; D006241:Haptens; D009363:Neoplasm Proteins; D009596:Nitrophenols; D010648:Phenylacetates; D005719:gamma-Globulins; C509258:tumor endothelial marker 1, mouse; C026242:4-hydroxy-5-nitrophenyl acetic acid