title: Immobilization of Pseudomonas cepacia lipase onto electrospun polyacrylonitrile fibers through physical adsorption and application to transesterification in nonaqueous solvent.
abstract: The lipase of Pseudomonas cepacia was immobilized onto electrospun polyacrylonitrile (PAN) fibers and used for the conversion of (S)-glycidol with vinyl n-butyrate to glycidyl n-butyrate in isooctane. The rate of reaction with the adsorbed lipase was 23-fold higher than the initial material. After 10 recyclings, the initial reaction rate was 80% of the original rate. This system of enzyme immobilization is therefore suitable for carrying out transesterification reactions in nonaqueous solvents.
authors.affiliation: Division of Chemical Engineering, Department of Materials Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan. sakai@cheng.es.osaka-u.ac.jp
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.forename: Yuping
authors.initials: Y
authors.lastname: Liu
authors.forename: Tetsu
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Rie
authors.initials: R
authors.lastname: Watanabe
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kawabe
authors.forename: Koei
authors.initials: K
authors.lastname: Kawakami
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D016956:Burkholderia cepacia / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000378:metabolism; D004951:Esterification; D007700:Kinetics; D012997:Solvents / Q000737:chemistry*; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D012997:Solvents; D014867:Water; C010504:polyacrylonitrile