title: Aromatase mRNA expression in the brain of adult Xenopus laevis exposed to Lambro river water and endocrine disrupting compounds.
abstract: Aromatase P450 (P450 arom; Cyp19) is a key enzyme for vertebrate reproduction and brain development that catalyzes the conversion of androgens to estrogens. The aim of this study was to improve the knowledge on EDC effects by analysing their potential impact on brain P450 arom in adult Xenopus laevis exposed for 4 weeks to an environmental sample, the water of the river Lambro (LAM), the most polluted tributary of the Po river in North Italy. Other groups were exposed to individual compounds 10(-8) M tamoxifen (TAM), ethinylestradiol (EE2), flutamide (FLU) and methyldihydrotestosterone (MDHT) known for their (anti)estrogenic and (anti)androgenic modes of action. Expression of CYP19 was evaluated in brain extracts by quantitative RT-PCR, using a pair of primers located in the open reading frame (ORF) that allowed the simultaneous amplification of all transcripts (Aro-ORF) and a pair of primers specific for brain aromatase (Aro-B). Significant increase in Aro-ORF and Aro-B mRNA levels were observed in both females and males exposed to LAM. Different changes were observed for the model compounds using two pairs of primers. Aro-ORF mRNA expression was significantly increased in EE2 and MDHT exposed males and in FLU-exposed females, while it was significantly decreased in TAM exposed females. Aro-B mRNA was significantly increased in both sexes exposed to FLU and decreased in TAM exposed females. In conclusion, aromatase mRNA in the brain of X. laevis was regulated differentially in a gender specific manner by certain (anti)estrogenic and (anti)androgenic EDCs, supporting previous hypotheses that diverse compounds present in the river Lambro may induce feminization and demasculinization effects.
authors.affiliation: Department of Biology, University of Genoa, Italy. massari@unige.it
authors.forename: A
authors.initials: A
authors.lastname: Massari
authors.forename: R
authors.initials: R
authors.lastname: Urbatzka
authors.forename: A
authors.initials: A
authors.lastname: Cevasco
authors.forename: L
authors.initials: L
authors.lastname: Canesi
authors.forename: C
authors.initials: C
authors.lastname: Lanza
authors.forename: L
authors.initials: L
authors.lastname: Scarabelli
authors.forename: W
authors.initials: W
authors.lastname: Kloas
authors.forename: A
authors.initials: A
authors.lastname: Mandich
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000633:toxicity*; D015971:Gene Expression Regulation, Enzymologic; D007558:Italy; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045483:Rivers; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014982:Xenopus laevis / Q000235:genetics*
chemical_list: D052244:Endocrine Disruptors; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D001141:Aromatase