Quantification of urinary PGEm, 6-keto PGF(1alpha) and 2,3-dinor-6-keto PGF(1alpha) by UFLC-MS/MS before and after exercise.
Eicosanoids play an important role in the evaluation of pro-inflammatory responses and in the safety and toxicity of novel therapeutic agents. This work describes a high-throughput UFLCMS/MS method for the analysis of three urinary prostanoid biomarkers of pro-inflammatory responses, tetranor PGEm, 6-keto PGF(1alpha) and 2,3-dinor-6-keto PFG(1alpha). Nine male volunteers of various age and fitness level participated in this study. Six provided pre- and post-exercise samples and three provided intraday samples. Tetranor PGEm and 6-keto PGF(1alpha) increased significantly in patients after exercise (p<0.017 and p<0.029). In individual patient sets, tetranor PGEm levels increased from 1.5- to 6-fold pre- vs. post-exercise, levels of 6-keto PGF(1alpha) increased more dramatically from 2- to 55-fold pre- vs. post-exercise. The prostanoid 2,3-dinor-6-keto PGF(1alpha) remained unchanged post-exercise. Data was normalized to urinary creatinine concentration, which increased approximately 40% post-exercise.
Blatnik
Matthew
M
Department of Pharmacokinetics, Dynamics & Metabolism, Pfizer Global Research and Development, Groton Laboratories. Eastern Point Rd, Groton, CT 06340, USA. matthew.blatnik@pfizer.com
Steenwyk
Rick C
RC
D015121:6-Ketoprostaglandin F1 alpha / Q000031:analogs & derivatives* / Q000652:urine*; D000328:Adult; D002853:Chromatography, Liquid; D003404:Creatinine / Q000652:urine; D015444:Exercise; D006801:Humans; D008297:Male; D008875:Middle Aged; D011453:Prostaglandins / Q000652:urine*; D053719:Tandem Mass Spectrometry
D011453:Prostaglandins; C532049:tetranor prostaglandin M; D015121:6-Ketoprostaglandin F1 alpha; C028290:2,3-dinor-6-ketoprostaglandin F1alpha; D003404:Creatinine