title: Two-dimensional reference map for the basic proteome of the human dorsolateral prefrontal cortex (dlPFC) of the prefrontal lobe region of the brain.
abstract: We describe a 2-DE proteomic reference map containing 227 basic proteins in the dorsolateral prefrontal cortex region of the human brain. Proteins were separated in the first dimension on pH 6-11 IPG strips using paper-bridge loading and on 12% SDS-PAGE in the second dimension. Proteins were subsequently identified by MS and spectra were analyzed using an in-house proteomics data analysis platform, Proline. The 2-DE reference map is available via the UCD 2-DE Proteome Database (http://proteomics-portal.ucd.ie:8082) and can also be accessed via the WORLD-2DPAGE Portal (http://www.expasy.ch/world-2dpage/). The associated protein identification data have been submitted to the PRIDE database (accession numbers 10018-10033). Separation of proteins in the basic region resolves more membrane associated proteins relevant to the synaptic pathology central to many neurological disorders. The 2-DE reference map will aid with further characterisation of neurological disorders such as bipolar and schizophrenia.
authors.affiliation: School of Medicine and Medical Science, University College Dublin, Dublin, Ireland. ciara.mcmanus@ucd.ie
authors.forename: Ciara A
authors.initials: CA
authors.lastname: McManus
authors.forename: Julie
authors.initials: J
authors.lastname: Polden
authors.forename: David R
authors.initials: DR
authors.lastname: Cotter
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Dunn
mesh_terms: D001923:Brain Chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D017397:Prefrontal Cortex / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*
chemical_list: D020543:Proteome