Identification of cellular proteins interacting with equine infectious anemia virus S2 protein.
The macrophage-tropic lentivirus, equine infectious anemia virus (EIAV), encodes the small auxiliary protein S2 from a short open reading frame that overlaps the amino terminus of env EIAV S2 is dispensable for virus replication in cultured cells but is required for disease production. S2 is approximately 7 kDa and has no overall amino acid sequence homology to other cellular or viral proteins. Therefore it is likely that S2 plays a role as an adaptor protein. To further investigate S2 function we performed a yeast-2-hybrid screen to identify cellular proteins that interact with EIAV S2. The screen identified two human cellular proteins, amplified in osteosarcoma (OS-9) and proteasome 26S ATPase subunit 3 (PSMC3) that interact with S2. The equine homologues of these proteins were cloned and their interactions with S2 confirmed using co-immunoprecipitation assays. We identified two OS-9 isoforms that interact with S2 and a third splice variant that does not, indicating a region of OS-9 apparently required for the S2 interaction. The roles of these cellular proteins during EIAV infection have not been determined.
Covaleda
Lina
L
Department of Pathobiology, Texas A&M University, Texas Veterinary Medical College, MS4467, College Station, TX 77843-4467, USA.
Gno
Bich-Ty
BT
Fuller
Fredrick J
FJ
Payne
Susan L
SL
D000818:Animals; D018397:Equidae; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D004860:Infectious Anemia Virus, Equine / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D025941:Protein Interaction Mapping; D017422:Sequence Analysis, DNA; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism*
C402643:S2 protein, Equine infectious anemia virus; D014764:Viral Proteins; D037521:Virulence Factors