title: Tissue reaction of the EndoREZ in root canal fillings short of or beyond an apical foramenlike communication.
abstract: This study evaluated the response of periapical tissues to the endodontic sealer EndoREZ in root canal fillings short of or beyond the apical foramenlike communication. Twenty root canals of premolars and incisors of 2 mongrel dogs were used. After coronal access and pulp extirpation, the canals were instrumented up to a size 55 K-file and the apical cemental barrier was penetrated with a size 15 K-file to create an apical foramenlike communication, which was widened to a size 25 K-file. The canals were irrigated with saline at each change of file. The root canals were obturated either short of or beyond the apical foramenlike opening by the lateral condensation of gutta-percha and EndoREZ, originating 2 experimental groups: G1, EndoREZ/short of the apical foramenlike opening, and G2, EndoREZ/beyond the apical foramenlike opening. The animals were killed by anesthetic overdose 90 days after endodontic treatment. The individual roots were obtained and serial histological sections were prepared for histomorphological analysis (H&E and Brown and Brenn techniques) under light microscopy. The following parameters were examined: closure of the apical foramenlike communication and apical opening of accessory canals, apical cementum resorptions, intensity of the inflammatory infiltrate, presence of giant cells, and thickness and organization of the apical periodontal ligament. Each parameter was scored 1 to 4, 1 being the best result and 4 the worst. Data were analyzed statistically by the Wilcoxon nonparametric tests (P = .05). Comparing the 2 groups, the best result (P = .05) was obtained with root canal filling with EndoREZ short of the apical foramenlike opening. In conclusion, limiting the filling material to the root canal space apically was important to determine the best treatment outcome when EndoREZ was used as the sealer.
authors.affiliation: Postgraduate Program in Endodontics, Dental School, University of Marília (UNIMAR), Marília, SP, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Suzuki
authors.forename: Valdir
authors.initials: V
authors.lastname: de Souza
authors.forename: Roberto
authors.initials: R
authors.lastname: Holland
authors.forename: Sueli Satomi
authors.initials: SS
authors.lastname: Murata
authors.forename: João Eduardo
authors.initials: JE
authors.lastname: Gomes-Filho
authors.forename: Eloi
authors.initials: E
authors.lastname: Dezan Junior
authors.forename: Thiago
authors.initials: T
authors.lastname: Rodrigues Dos Passos
mesh_terms: D000818:Animals; D056624:Apexification / Q000379:methods; D001641:Bicuspid / Q000473:pathology; D003188:Composite Resins / Q000627:therapeutic use*; D003723:Dental Amalgam; D003739:Dental Cementum / Q000187:drug effects; D003786:Dental Pulp Cavity / Q000473:pathology*; D003793:Dental Restoration, Permanent / Q000379:methods; D004285:Dogs; D005547:Foreign Bodies / Q000139:chemically induced; D015726:Giant Cells / Q000473:pathology; D005899:Glass Ionomer Cements; D006171:Gutta-Percha / Q000627:therapeutic use; D007180:Incisor / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008422:Materials Testing; D010486:Periapical Tissue / Q000187:drug effects*; D010513:Periodontal Ligament / Q000187:drug effects / Q000473:pathology; D012387:Root Canal Filling Materials / Q000627:therapeutic use*; D012389:Root Canal Obturation / Q000379:methods; D018915:Root Canal Preparation / Q000379:methods; D019227:Tooth Apex / Q000187:drug effects / Q000473:pathology*
chemical_list: D003188:Composite Resins; C481259:EndoRez; D005899:Glass Ionomer Cements; D012387:Root Canal Filling Materials; D003723:Dental Amalgam; D006171:Gutta-Percha