title: In vivo administration of acepromazine or promethazine to horse decreases the reactive oxygen species production response of subsequently isolated neutrophils to stimulation with phorbol myristate acetate.
abstract: The previous experiments have shown that some phenothiazines have antioxidant and anti-inflammatory properties in vitro. In this study the inhibition of the production of reactive oxygen species (ROS) by neutrophils was studied in two groups of horses, which received a dose of 0.1 mg/kg of either acepromazine or promethazine intravenously. Blood samples were collected before (T0) and 0.5, 1, 3 and 5 h after drug administration. The chemiluminescence (CML) response of neutrophils was measured ex vivo in the presence of luminol for a period of 10 min and the maximum CML value (peak value) recorded. There was a significant inhibition of the ROS production in the acepromazine treated group (49% inhibition) at 5 h after administration and in the promethazine group (24% inhibition) at 3 h after administration (P < 0.05 vs. T0). These findings are of therapeutic relevance in the use of phenothiazines in equine patients with inflammatory diseases where neutrophil activation and ROS production are implicated.
authors.affiliation: University of Liège, Faculty of Veterinary Medicine, Equine Clinic, B41, 4000 Liège 1, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Péters
authors.forename: T
authors.initials: T
authors.lastname: Franck
authors.forename: M
authors.initials: M
authors.lastname: Pequito
authors.forename: G
authors.initials: G
authors.lastname: de la Rebière
authors.forename: S
authors.initials: S
authors.lastname: Grulke
authors.forename: A
authors.initials: A
authors.lastname: Salccicia
authors.forename: D
authors.initials: D
authors.lastname: Verwilghen
authors.forename: L
authors.initials: L
authors.lastname: Chiavaccini
authors.forename: G
authors.initials: G
authors.lastname: Deby-Dupont
authors.forename: D
authors.initials: D
authors.lastname: Serteyn
mesh_terms: D000075:Acepromazine / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D018492:Dopamine Antagonists / Q000494:pharmacology; D006736:Horses; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011398:Promethazine / Q000008:administration & dosage / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D018492:Dopamine Antagonists; D017382:Reactive Oxygen Species; D000075:Acepromazine; D011398:Promethazine; D013755:Tetradecanoylphorbol Acetate