T- and B-cell-mediated protection induced by novel, live attenuated pertussis vaccine in mice. Cross protection against parapertussis.
BACKGROUND
Despite the extensive use of efficacious vaccines, pertussis still ranks among the major causes of childhood mortality worldwide. Two types of pertussis vaccines are currently available, whole-cell, and the more recent acellular vaccines. Because of reduced reactogenicity and comparable efficacy acellular vaccines progressively replace whole-cell vaccines. However, both types require repeated administrations for optimal efficacy. We have recently developed a live attenuated vaccine candidate, named BPZE1, able to protect infant mice after a single nasal administration.


RESULTS
We determined the protective mechanism of BPZE1-mediated immunity by using passive transfer of T cells and antibodies from BPZE1-immunized mice to SCID mice. Clearance of Bordetella pertussis from the lungs was mediated by both BPZE1-induced antibodies and CD4(+), but not by CD8(+) T cells. The protective CD4(+) T cells comprised IFN-gamma-producing and IL-17-producing subsets, indicating that BPZE1 induces both Th1 and Th17 CD4(+) T cells. In addition, and in contrast to acellular pertussis vaccines, BPZE1 also cross-protected against Bordetella parapertussis infection, but in this case only the transfer of CD4(+) T cells conferred protection. Serum from BPZE1-immunized mice was not able to kill B. parapertussis and did not protect SCID mice against B. parapertussis infection.


CONCLUSIONS
The novel live attenuated pertussis vaccine BPZE1 protects in a pre-clinical mouse model against B. pertussis challenge by both BPZE1-induced antibodies and CD4(+) T cell responses. It also protects against B. parapertussis infection. However, in this case protection is only T cell mediated.
Feunou
Pascal Feunou
PF
Inserm U1019, Lille, France.
Bertout
Julie
J
Locht
Camille
C
D000281:Administration, Intranasal; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D056738:Cross Protection; D004353:Drug Evaluation, Preclinical; D051379:Mice; D010567:Pertussis Vaccine / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology*; D014613:Vaccines, Attenuated
D010567:Pertussis Vaccine; D014613:Vaccines, Attenuated