[Protective effects of sini decoction on adriamycin-induced heart failure and its mechanism].
OBJECTIVE
To investigate the protective effects of Sini decoction (SND) on Adriamycin-induced heart failure and its mechanism.


METHODS
SD rats were randomly divided into three groups:control group,heart failure model group and SND group. ADR was injected in to the rats of model group and SND group by caudal vein. After injection,the rats in SND group were given SND [3.75 g/(kg x d), p.o.]. Three weeks later, protein expressions of Bid and Bcl-xl were detected by immunohistochemistry; mRNA expression ratio of Bcl-xl/Bcl-xs was detected by RT-PCR and apoptosis rate was determinated by flow cytometry.


RESULTS
Compared with control group, the protein expression of Bcl-xl and mRNA ratio of Bcl-xl/Bcl-xs obviously decreased,while the protein expression of Bid and apoptosis rate significantly increased in the model group. SND could decrease cell apoptosis, increase the protein expression of Bcl-xl, increase bcl-xl/bcl-xs mRNA ratio and decrease Bid protein expression.


CONCLUSIONS
Bcl-xl may play an important role in ADR-induced heart failure rats. The mechanism of SND on protecting cardiocyte may be related to apoptosis correlation factor, Bcl-xl and Bid.
Zhao
Ming-qi
MQ
Guangzhou women and Children's Medical Center, Guangzhou 510120, China.
Wu
Wei-kang
WK
Zhao
Dan-yang
DY
Duan
Xin-fen
XF
Liu
Ying
Y
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051023:BH3 Interacting Domain Death Agonist Protein / Q000235:genetics / Q000378:metabolism; D004317:Doxorubicin; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005434:Flow Cytometry; D006333:Heart Failure / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D006334:Heart Function Tests; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051020:bcl-X Protein / Q000235:genetics / Q000378:metabolism*
D051023:BH3 Interacting Domain Death Agonist Protein; C494813:Bcl2l1 protein, rat; C494816:Bid protein, rat; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D051020:bcl-X Protein; C478756:sini tang; D004317:Doxorubicin