Monoclonal antibodies MC3 and MC5 can be used as a tool for screening colorectal cancer.
MC3 and MC5 are both colorectal cancer-specific MAbs previously prepared in our laboratory that can detect colorectal cancer with high sensitivity and specificity. Thus far the distribution of MC3-Ag and MC5-Ag in colorectal cancer remains largely unknown. In the present study, we have firstly found that the expression of MC3-Ag and MC5-Ag was higher in moderate-differentiated and poor-differentiated colorectal cancer tissue than that in well-differentiated colorectal cancer tissue by immunohistochemistry. The expression of MC5-Ag in colorectal cancer tissue without metastasis was found to be significantly less than that in tissue accompanied with metastasis. However, the expression of MC3-Ag in colorectal cancer tissue without metastasis was found similar to that in tissue accompanied with metastasis. The results showed that MC3-Ag and MC5-Ag might play important roles in colorectal carcinogenesis and that MC3 and MC5 could be used as a tool for screening colorectal cancer.
Zhou
Yi
Y
Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
Zhang
Xipeng
X
Hua
Yang
Y
Zhao
Qingchuan
Q
Wang
Xin
X
Fan
Daiming
D
D000911:Antibodies, Monoclonal; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000276:immunology*; D055088:Early Detection of Cancer / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D009362:Neoplasm Metastasis / Q000276:immunology; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal