title: Detection of indoor and outdoor avian antigen in management of bird-related hypersensitivity pneumonitis.
abstract: BACKGROUND
  In the management of hypersensitivity pneumonitis (HP), antigen avoidance is crucial to prevent the progression of disease. Indirect and unrecognized exposure to the antigen may continue for a long time if persistence of the causative antigen is not recognized. To make a correct assessment of the patients' environment, we tried to establish the methods to detect indoor and outdoor avian antigens.
  
  
  METHODS
  Sixteen patients with bird-related HP, 4 asymptomatic breeders, and 6 healthy controls were examined. We prepared anti-pigeon dropping extracts (PDE) polyclonal antibody from rabbits. Air samples and house dust samples were analyzed by an antigen-capture ELISA with signal amplification using catalyzed reporter deposition.
  
  
  RESULTS
  In air samples, avian antigen could be detected in patients with HP (0.73 +/- 0.53 ng/m3) and asymptomatic breeders (0.63 +/- 0.23 ng/m3). In house dust samples, the amount of avian antigen was higher in patients with HP (2.4 +/- 1.8 microg/g) and asymptomatic breeders (4.1 +/- 2.3 microg/g) than in the controls (0.1 +/- 0.2 microg/g).
  
  
  CONCLUSIONS
  Detection of indoor and outdoor avian antigen might contribute to the correct diagnosis and appropriate managements of bird-related HP.
authors.affiliation: Department of Integrated Pulmonology, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Jin
authors.initials: J
authors.lastname: Kuramochi
authors.forename: Naohiko
authors.initials: N
authors.lastname: Inase
authors.forename: Koji
authors.initials: K
authors.lastname: Takayama
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Yoshizawa
mesh_terms: D000388:Air / Q000032:analysis; D016902:Air Pollution, Indoor / Q000009:adverse effects*; D000542:Alveolitis, Extrinsic Allergic / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology* / Q000628:therapy; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry* / Q000276:immunology; D010856:Columbidae / Q000276:immunology; D045424:Complex Mixtures; D004391:Dust / Q000032:analysis; D004781:Environmental Exposure / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005240:Feasibility Studies; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D011817:Rabbits
chemical_list: D030161:Avian Proteins; D045424:Complex Mixtures; D004391:Dust