Differentiation of cryopreserved human umbilical cord blood-derived stromal cells into cells with an oligodendrocyte phenotype.
In this study, we examined the phenotypic characteristics of human umbilical cord blood-derived mesenchymal stromal cells (UCB-derived MSCs) differentiated along an oligodendrocyte pathway. We induced human UCB-derived MSCs to form floating neurospheres, and these neurospheres were then induced to differentiate into oligodendrocyte progenitor-like cells using multiple induction factors. Differentiated UCB-derived MSCs showed morphologic characteristics of an oligodendrocyte phenotype. The expression of cell surface markers characteristic of oligodendrocyte progenitor cells or oligodendrocytes was determined by immunocytochemical staining. These results suggest that human UCB-derived MSCs can be induced to differentiate into cells with an oligodendrocyte phenotype and that these cells may have potential in the future cellular therapy of central neurological disorders.
Luo
Yong-Chun
YC
Department of Neurosurgery, Military General Hospital of Beijing PLA, Beijing 100700, China.
Zhang
Hong-Tian
HT
Cheng
Hao-Yu
HY
Yang
Zhi-Jun
ZJ
Dai
Yi-Wu
YW
Xu
Ru-Xiang
RX
D002454:Cell Differentiation; D048430:Cell Shape; D015925:Cryopreservation / Q000379:methods*; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology*; D010641:Phenotype; D017154:Stromal Cells / Q000166:cytology