Inhibitory activity of blasticidin A, a strong aflatoxin production inhibitor, on protein synthesis of yeast: selective inhibition of aflatoxin production by protein synthesis inhibitors.
Blasticidin A (BcA), an antibiotic produced by Streptomyces, inhibits aflatoxin production without strong growth inhibition toward aflatoxin-producing fungi. During the course of our study on the mode of action of BcA by two-dimensional differential gel electrophoresis (2D-DIGE), we found a decrease in the abundances of ribosomal proteins in Saccharomyces cerevisiae after exposure to BcA. This phenomenon was also observed by treatment with blasticidin S (BcS) or cycloheximide. BcA inhibited protein synthesis in a galactose-induced expression system in S. cerevisiae similar to BcS and cycloheximide. BcS, but not cycloheximide, inhibited aflatoxin production in Aspergillus parasiticus without inhibition of fungal growth, similar to BcA. A decrease in the abundances of aflatoxin biosynthetic enzymes was observed in 2D-DIGE experiments with Aspergillus flavus after exposure to BcA or BcS. These results suggested that protein synthesis inhibitors are useful to control aflatoxin production.
Yoshinari
Tomoya
T
Department of Applied Biological Chemistry, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Noda
Yoichi
Y
Yoda
Koji
K
Sezaki
Hiroshi
H
Nagasawa
Hiromichi
H
Sakuda
Shohei
S
D000348:Aflatoxins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D001230:Aspergillus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D009705:Nucleosides / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D011760:Pyrrolidinones / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D000348:Aflatoxins; D009705:Nucleosides; D011500:Protein Synthesis Inhibitors; D011760:Pyrrolidinones; C420726:blasticidin A; C004500:blasticidin S; D003513:Cycloheximide