title: Electricity generation from glucose by a Klebsiella sp. in microbial fuel cells.
abstract: As electrochemically active bacteria play an important role in microbial fuel cells (MFCs), it is necessary to get a comprehensive understanding of their electrogenesis mechanisms. In this study, a new electrochemically active bacterium, Klebsiella sp. ME17, was employed into an "H" typed MFC for electrogenesis, with glucose as the electron donor. The maximum power density was 1,209 mW/m2 at a resistance of 340 Omega and the maximum current was 1.47 mA. Given the original anode medium, fresh medium, and the supernatant of the anode medium in the same MFC, respectively, the polarization curves illustrated that the strain produced mediators to promote extracellular electron transfer. The anode medium supernatant was electrochemically active, based on cyclic voltammogram, and the supernatant was very likely to contain quinone-like substances, as indicated by spectrophotometric and excitation-emission matrix fluorescence spectroscopy analysis. Further investigation on the color and ultraviolet absorbance at 254 nm of the filtered anode medium showed that the redox states of mediators strongly associated with the electricity generation states in MFCs.
authors.affiliation: Department of Environmental Science and Engineering, State Key Joint Laboratory of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Xia
authors.forename: Xiao-xin
authors.initials: XX
authors.lastname: Cao
authors.forename: Peng
authors.initials: P
authors.lastname: Liang
authors.forename: Xia
authors.initials: X
authors.lastname: Huang
authors.forename: Su-ping
authors.initials: SP
authors.lastname: Yang
authors.forename: Gen-gui
authors.initials: GG
authors.lastname: Zhao
mesh_terms: D001674:Bioelectric Energy Sources / Q000382:microbiology*; D004560:Electricity; D005947:Glucose / Q000378:metabolism*; D007709:Klebsiella / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D005947:Glucose