Baculovirus as a gene delivery vector for cartilage and bone tissue engineering.
Baculovirus is an effective vector for gene delivery into various mammalian cells, including chondrocytes and mesenchymal stem cells, and has been employed for diverse applications. By gene delivery and expression of the growth factor, recombinant baculovirus has been shown to modulate the differentiation state of the cells and stimulates the production of extracellular matrix and tissue formation, hence repairing the damaged cartilage and bone in vivo. This article reviews the studies pertaining to the applications of baculovirus-mediated gene delivery in cartilage and bone tissue engineering and discusses recent progress, future applications and potential hurdles.
Lin
Chin-Yu
CY
Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.
Lu
Chia-Hsin
CH
Luo
Wen-Yi
WY
Chang
Yu-Han
YH
Sung
Li-Yu
LY
Chiu
Hsin-Yi
HY
Hu
Yu-Chen
YC
D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism; D002356:Cartilage / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism; D018014:Gene Transfer Techniques; D005796:Genes; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*