Herbidospora sakaeratensis sp. nov., isolated from soil, and reclassification of Streptosporangium claviforme as a later synonym of Herbidospora cretacea.
An actinomycete strain, DMKUA 205(T), was isolated from a soil sample collected from the Sakaerat Biosphere Reserve in Nakhonratchasima Province, Thailand. The novel strain produced short chains of non-motile spores on the tips of long sporophores branching from the vegetative hyphae. The morphological and chemotaxonomic properties of this new isolate corresponded to those of members of the genus Herbidospora. Furthermore, 16S rRNA gene sequence analysis showed that the strain was closely related to members of the genus Herbidospora. Phenotypic properties and DNA-DNA relatedness values differentiated the new strain from its closest phylogenetic relatives Herbidospora yilanensis 0351M-12(T) (35-54 % DNA-DNA relatedness) and Herbidospora daliensis 0385M-1(T) (58-65 % relatedness). On the basis of phenotypic, genotypic and phylogenetic data, strain DMKUA 205(T) could be clearly distinguished from the type strains of H. yilanensis and H. daliensis. Therefore, strain DMKUA 205(T) represents a novel species, for which the name Herbidospora sakaeratensis sp. nov. is proposed. The type strain is strain DMKUA 205(T) ( = BCC 11662(T) = NBRC 102641(T)). In addition, the DNA-DNA hybridization results from this study revealed that Streptosporangium claviforme is a later synonym of Herbidospora cretacea.
Boondaeng
Antika
A
Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Suriyachadkun
Chanwit
C
BIOTEC Culture Collection National Center for Genetic Engineering and Biotechnology, Pathumthani 12120, Thailand.
Ishida
Yuumi
Y
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, Chiba 292-0818, Japan.
Tamura
Tomohiko
T
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, Chiba 292-0818, Japan.
Tokuyama
Shinji
S
Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
Kitpreechavanich
Vichien
V
Center for Advanced Studies in Tropical Natural Resources, KU Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand.|Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000166:cytology; D013785:Thailand
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S