title: Meat species identification and Halal authentication analysis using mitochondrial DNA.
abstract: A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the mitochondrial genes was developed for beef (Bos taurus), pork (Sus scrofa), buffalo (Bubalus bubali), quail (Coturnix coturnix), chicken (Gallus gallus), goat (Capra hircus), rabbit (Oryctolagus cuniculus) species identification and Halal authentication. PCR products of 359-bp were successfully obtained from the cyt b gene of these six meats. AluI, BsaJI, RsaI, MseI, and BstUI enzymes were identified as potential restriction endonucleases to differentiate the meats. The genetic differences within the cyt b gene among the meat were successfully confirmed by PCR-RFLP. A reliable typing scheme of species which revealed the genetic differences among the species was developed.
authors.affiliation: School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan.
authors.forename: Chandrika
authors.initials: C
authors.lastname: Murugaiah
authors.forename: Zainon Mohd
authors.initials: ZM
authors.lastname: Noor
authors.forename: Maimunah
authors.initials: M
authors.lastname: Mastakim
authors.forename: Lesley Maurice
authors.initials: LM
authors.lastname: Bilung
authors.forename: Jinap
authors.initials: J
authors.lastname: Selamat
authors.forename: Son
authors.initials: S
authors.lastname: Radu