title: Toxicity of waterborne Copper in premetamorphic tadpoles of Lithobates catesbeianus (Shaw, 1802).
abstract: Toxicity parameters of Copper to pre-metamorphic larvae of Lithobates catesbeianus were evaluated in laboratory conditions. The acute toxicity (as LC-50 96 h) was 3.96 mg Cu(2+) L(-1) (95% confidence interval: 3.21-4.89); the bioconcentration of the metal after 96 h exposure followed an exponential increase. The potential genotoxicity effect evaluated with Micronucleus Test showed a reduced sensitivity of the animals to the assayed concentrations of the metal, exhibiting only a modest increase in the frequency of erythrocytes micronuclei, meanwhile larvae exposed to cyclophosphamide (positive control) showed significant increases. The Condition Factor was significantly reduced while the Hepatosomatic Index remained unaltered.
authors.affiliation: Programa de Ecofisiología Aplicada (PRODEA-INEDES), Departamento de Ciencias Básicas, Universidad Nacional de Luján, Casilla de Correo 221, B6700ZBA Luján, Argentina.
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Ossana
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Castañé
authors.forename: Gisela L
authors.initials: GL
authors.lastname: Poletta
authors.forename: Marta D
authors.initials: MD
authors.lastname: Mudry
authors.forename: Alfredo
authors.initials: A
authors.lastname: Salibián
mesh_terms: D000818:Animals; D003300:Copper / Q000493:pharmacokinetics / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D007928:Lethal Dose 50; D008675:Metamorphosis, Biological / Q000187:drug effects*; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D015162:Micronucleus Tests; D011898:Ranidae; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D003300:Copper