title: Overexpression of miR-152 leads to reduced expression of human leukocyte antigen-G and increased natural killer cell mediated cytolysis in JEG-3 cells.
abstract: OBJECTIVE
  The purpose of this study was to gain a further understanding of the relationship between miR-152 and human leukocyte antigen (HLA)-G in human trophoblast cell line (JEG-3).
  
  
  METHODS
  The JEG-3 cells were transfected with pre-miR-152. The effect of the overexpressed miR-152 on HLA-G expression, trophoblast invasion, and natural killer (NK) cell-mediated cytolysis were assessed by reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, transwell invasion assay, and NK cell cytotoxicity assay, respectively.
  
  
  RESULTS
  The miR-152 repressed HLA-G expression but exerted no effect on JEG-3 cell invasion, and overexpression of miR-152 led to increased NK cell-mediated cytolysis in JEG-3 cells.
  
  
  CONCLUSIONS
  The data indicate that miR-152 may function as an immune system enhancer through up-regulating NK cell-mediated cytolysis of host cells.
authors.affiliation: Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China. xiaomingzhu1981@hotmail.com
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Zhu
authors.forename: Tao
authors.initials: T
authors.lastname: Han
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Wang
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Li
authors.forename: Hua-guang
authors.initials: HG
authors.lastname: Yang
authors.forename: Ya-ning
authors.initials: YN
authors.lastname: Luo
authors.forename: Guo-wu
authors.initials: GW
authors.lastname: Yin
authors.forename: Yuan-qing
authors.initials: YQ
authors.lastname: Yao
mesh_terms: D000704:Analysis of Variance; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000235:genetics / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D020506:Untranslated Regions / Q000235:genetics / Q000276:immunology
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D035683:MicroRNAs; D020506:Untranslated Regions