Hollow-fiber ultrafiltration and PCR detection of human-associated genetic markers from various types of surface water in Florida.
Hollow-fiber ultrafiltration (HFUF) and PCR were combined to detect human-associated microbial source tracking marker genes in large volumes of fresh and estuarine Florida water. HFUF allowed marker detection when membrane filtration did not, demonstrating HFUF's ability to facilitate detection of diluted targets by PCR in a variety of water types.
Leskinen
Stephaney D
SD
Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, Florida 33620, USA.
Brownell
Miriam
M
Lim
Daniel V
DV
Harwood
Valerie J
VJ
D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D005431:Florida; D005819:Genetic Markers; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D014462:Ultrafiltration / Q000379:methods*; D014871:Water Microbiology
D004269:DNA, Bacterial; D005819:Genetic Markers