Relationships between reproductive characteristics in male Vimba vimba L. and the effects of osmolality on sperm motility.
In Vimba vimba, GSI, sperm volume, and spermatozoa concentration range from 3.4-7.4 %, 0.1-1.1 ml, and 13.3-34.8 x 10(9) spz ml(-1), respectively. Gonad mass (r = 0.90) and sperm volume (r = 0.35) significantly correlated with weight of males. Significant correlation was also found between gonad mass and length of males (r = 0.85). Sperm motility (r = 0.99) and velocity (r = 098) significantly decreased after activation in Tris-HCl 20 mM, pH 8.5. Osmolality of the seminal plasma was 273.2 mOsmol kg(-1). Sperm motility and velocity were significantly affected by the osmolality of the activation medium (P < 0.01). Hyper-osmolality compared to seminal plasma osmolality totally suppressed the sperm activation. At 15 s post-activation, the sperm motility significantly decreased at 240 mOsmol kg(-1) in KCl or NaCl media. The highest sperm motility and velocity (at 60 s post-activation) were observed at 200 mOsmol kg(-1) in NaCl, KCl, or sucrose media. In all treatments, the tip of the flagellum of spermatozoa became curled into a loop shape after activation of sperm in distilled water containing 20 mM Tris-HCl, pH 8.5 that shortened the flagellum.
Alavi
S M H
SM
University of South Bohemia, Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic. alavi@vurh.jcu.cz
Kozak
P
P
Hatef
A
A
Hamackova
J
J
Linhart
O
O
D000818:Animals; D049628:Body Size; D018929:Cell Culture Techniques; D003470:Culture Media; D003530:Cyprinidae / Q000033:anatomy & histology / Q000502:physiology*; D008297:Male; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000494:pharmacology; D012098:Reproduction; D012661:Semen / Q000187:drug effects; D012726:Sexual Behavior, Animal; D012965:Sodium Chloride / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
D003470:Culture Media; D012965:Sodium Chloride; D011189:Potassium Chloride