title: Investigations of fungal secondary metabolites with potential anticancer activity.
abstract: Fourteen metabolites, isolated from phytopathogenic and toxigenic fungi, were evaluated for their in vitro antigrowth activity for six distinct cancer cell lines, using the MTT colorimetric assay. Bislongiquinolide (1) and dihydrotrichodimerol (5), which belong to the bisorbicillinoid structural class, displayed significant growth inhibitory activity against the six cancer cell lines studied, while the remaining compounds displayed weak or no activity. The data show that 1 and 5 have similar growth inhibitory activities with respect to those cancer cell lines that display certain levels of resistance to pro-apoptotic stimuli or those that are sensitive to apoptosis. Quantitative videomicroscopy analysis revealed that 1 and 5 exert their antiproliferative effect through cytostatic and not cytotoxic activity. The preliminary results from the current study have stimulated further structure-activity investigations with respect to the growth inhibitory activity of compounds belonging to the bisorbicillinoid group.
authors.affiliation: Laboratoire de Toxicologie and Laboratoire de Chimie Analytique, Toxicologie et Chimie Physique Appliquee, Institut de Pharmacie, Universite Libre de Bruxelles (ULB), Brussels, Belgium.
authors.forename: ElHadj Saidou
authors.initials: ES
authors.lastname: Balde
authors.forename: Anna
authors.initials: A
authors.lastname: Andolfi
authors.forename: Céline
authors.initials: C
authors.lastname: Bruyère
authors.forename: Alessio
authors.initials: A
authors.lastname: Cimmino
authors.forename: Delphine
authors.initials: D
authors.lastname: Lamoral-Theys
authors.forename: Maurizio
authors.initials: M
authors.lastname: Vurro
authors.forename: Marc Van
authors.initials: MV
authors.lastname: Damme
authors.forename: Claudio
authors.initials: C
authors.lastname: Altomare
authors.forename: Véronique
authors.initials: V
authors.lastname: Mathieu
authors.forename: Robert
authors.initials: R
authors.lastname: Kiss
authors.forename: Antonio
authors.initials: A
authors.lastname: Evidente
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001231:Aspergillus flavus / Q000737:chemistry*; D001643:Bridged Bicyclo Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D005658:Fungi / Q000737:chemistry; D006801:Humans; D051379:Mice; D018715:Microscopy, Video; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D014242:Trichoderma / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; C473618:bisorbibutenolide; C508028:tetrahydrotrichodimerol; D015107:4-Butyrolactone