[Effect of non-contact coculture on bone marrow stromal cells and neural stem cells].
OBJECTIVE
To observe the effect of non-contact coculture on bone marrow stromal cells (MSCs) and neural stem cells (NSCs) in neural stem cell culture medium.


METHODS
MSCs and NSCs were cultured in non-contact coculture in Transwell plate, and cell morphology and immunocytochemical profile were investigated.


RESULTS
In the coculture, the NSCs showed adhering growth and extended long processes, and the migrating cells formed a network of cells within 7 days. The cells differentiated into neurons, astrocytes and oligodendrocytes as shown by immunocytochemistry. Most of the MSCs grew in a non-adherent manner, giving rise to large spherical cell masses which expressed neuronal, astrocyte, and oligodendrocyte phenotypes. In the control group, the NSCs grew in suspension, some of MSCs formed small non-adherent spherical cell masses, while some cells showed adherent growth.


CONCLUSIONS
MSCs and NSCs in the non-contact coculture can mutually promote the cell differentiation into neural cells in neural stem cell culture medium, indicating that both MSCs and NSCs can secrete some neurotrophic factors to provide a microenvironment suitable for survival and differentiation for each other.
Yang
Jun
J
Department of Neurology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. yangweixiao222@yahoo.com.cn
Yang
Qin
Q
Jia
Yan-jie
YJ
Li
Ao
A
Zeng
Zhi-lei
ZL
Xie
Peng
P
Ren
Jun-wei
JW
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D005260:Female; D008297:Male; D058953:Neural Stem Cells / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology