Proliferative activity of cells from remaining dental pulp in response to treatment with dental materials.
BACKGROUND
The biological examination of pulp injury, repair events and response of dental pulp stem cells to dental restorative materials is important to accomplish restorative treatment, especially to commonly used dental materials in paediatric dentistry, such as glass ionomer cement (GIC) and calcium hydroxide (Ca(OH)(2)) lining cement.


METHODS
Healthy patients aged between 9 to 11 years with carious primary molars without pulp exposure were selected and divided into two groups: Group 1 (teeth restored with GIC) and Group 2 (teeth lined using Ca(OH)(2) and restored with GIC). The proliferative activity of stem cells of teeth between these two groups was compared using colourimetric cell proliferation reagent, alamarBlue. Immunocytochemistry and flow cytometry confirmation were performed using mesenchymal stem cell markers, CD105 and CD166.


RESULTS
The proliferative activity using alamarBlue assay showed that cells derived from the remaining dental pulp of exfoliated deciduous teeth were positive for CD105 and CD166 and exhibited no difference between the two groups.


CONCLUSIONS
It can be concluded that the use of Ca(OH)(2) or GIC as a lining material in indirect pulp capping procedures has the same effect on cells derived from the remaining dental pulp of exfoliated deciduous teeth which have responded favourably to the restorative treatments.
Lutfi
A N
AN
School of Dental Sciences, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia.
Kannan
T P
TP
Fazliah
M N
MN
Jamaruddin
M A
MA
Saidi
J
J
D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002126:Calcium Hydroxide / Q000627:therapeutic use*; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis; D049109:Cell Proliferation / Q000187:drug effects; D002648:Child; D003124:Colorimetry; D003731:Dental Caries / Q000628:therapy; D003736:Dental Cavity Lining; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D003793:Dental Restoration, Permanent / Q000379:methods*; D000071063:Endoglin; D005326:Fetal Proteins / Q000032:analysis; D005434:Flow Cytometry; D005500:Follow-Up Studies; D005899:Glass Ionomer Cements; D006801:Humans; D007150:Immunohistochemistry; D007202:Indicators and Reagents; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000473:pathology; D008963:Molar / Q000473:pathology; D010078:Oxazines; D011956:Receptors, Cell Surface / Q000032:analysis; D013234:Stem Cells / Q000187:drug effects*; D014094:Tooth, Deciduous / Q000473:pathology; D014966:Xanthenes; D020410:Activated-Leukocyte Cell Adhesion Molecule
D015703:Antigens, CD; D015415:Biomarkers; D002126:Calcium Hydroxide; D015816:Cell Adhesion Molecules, Neuronal; D000071063:Endoglin; D005326:Fetal Proteins; D005899:Glass Ionomer Cements; D007202:Indicators and Reagents; D010078:Oxazines; D011956:Receptors, Cell Surface; D014966:Xanthenes; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule; C064206:ENG protein, human; C005843:resazurin