The nucleotide hydrolysis is altered in blood serum of streptozotocin-induced diabetic rats.
Ectonucleotidases and the nucleotide metabolism have been implicated as important regulators of various tissue functions in diabetes disease. Here we evaluated the ectonucleotidase activities and the profile of extracellular ATP metabolism in blood serum of streptozotocin (STZ)-induced diabetic rats. We observed a raise in ATP, ADP, AMP, and 5'-TMP hydrolysis in blood serum after 30 days of diabetes induction, when compared with the citrate group. However, in serum of rats treated 6 days with insulin, the hydrolysis returned to the control levels. Extracellular ATP metabolism estimated by HPLC analysis showed a rapid hydrolysis of extracellular ATP by diabetic animals, leading to the formation of high levels of adenosine when compared with citrate and insulin groups. Since in diabetes the vascular disease is frequently present, the alterations observed are important, because these enzymes control the nucleotides/nucleosides ratio in the circulation and thus the events related to haemostasis.
Rücker
Bárbara
B
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Abreu-Vieira
Gustavo
G
Bischoff
Lívia B
LB
Harthmann
Angela D'A
AD
Sarkis
João J F
JJ
Wink
Márcia R
MR
Casali
Emerson A
EA
D000241:Adenosine / Q000378:metabolism; D000251:Adenosine Triphosphatases; D000818:Animals; D003920:Diabetes Mellitus; D003924:Diabetes Mellitus, Type 2; D006868:Hydrolysis; D008297:Male; D009711:Nucleotides / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D044967:Serum / Q000378:metabolism*; D013311:Streptozocin
D009711:Nucleotides; D013311:Streptozocin; D000251:Adenosine Triphosphatases; C027201:ectoATPase; D000241:Adenosine