title: A sensitive method to extract DNA from biological traces present on ammunition for the purpose of genetic profiling.
abstract: Exploring technological limits is a common practice in forensic DNA research. Reliable genetic profiling based on only a few cells isolated from trace material retrieved from a crime scene is nowadays more and more the rule rather than the exception. On many crime scenes, cartridges, bullets, and casings (jointly abbreviated as CBCs) are regularly found, and even after firing, these potentially carry trace amounts of biological material. Since 2003, the Forensic Laboratory for DNA Research is routinely involved in the forensic investigation of CBCs in the Netherlands. Reliable DNA profiles were frequently obtained from CBCs and used to match suspects, victims, or other crime scene-related DNA traces. In this paper, we describe the sensitive method developed by us to extract DNA from CBCs. Using PCR-based genotyping of autosomal short tandem repeats, we were able to obtain reliable and reproducible DNA profiles in 163 out of 616 criminal cases (26.5%) and in 283 out of 4,085 individual CBC items (6.9%) during the period January 2003-December 2009. We discuss practical aspects of the method and the sometimes unexpected effects of using cell lysis buffer on the subsequent investigation of striation patterns on CBCs.
authors.affiliation: Forensic Laboratory for DNA Research, Department of Human Genetics, Leiden University Medical Center, Postzone S-05-P, PO Box 9600, 2300 RC Leiden, The Netherlands.
authors.forename: Patrick
authors.initials: P
authors.lastname: Dieltjes
authors.forename: René
authors.initials: R
authors.lastname: Mieremet
authors.forename: Sofia
authors.initials: S
authors.lastname: Zuniga
authors.forename: Thirsa
authors.initials: T
authors.lastname: Kraaijenbrink
authors.forename: Jeroen
authors.initials: J
authors.lastname: Pijpe
authors.forename: Peter
authors.initials: P
authors.lastname: de Knijff
mesh_terms: D003415:Crime / Q000331:legislation & jurisprudence*; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D005389:Firearms; D056426:Genetic Loci / Q000235:genetics; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D009426:Netherlands; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D004247:DNA