[Effects of human tissue kallikrein gene transfer on the migration of vascular smooth muscule cells].
OBJECTIVE
To investigate the effects of adenovirus-mediated human tissue kallikrein (Ad-hKLK1) gene transfer on platelet-derived growth factor-BB (PDGF-BB)-induced migration of vascular smooth muscle cells from spontaneously hypertensive rats (VSMC(SHR)).


METHODS
A bicistronic recombinant adenovirus vector (Ad-hKLK1) carrying the target hKLK1 gene and the reporter gene EGFP was constructed. VSMCs isolated from the thoracic aorta of male SHR were passaged, and the quiescent VSMC(SHR) in passages 3-6 seeded in 6-well plates were treated with Ad-hKLK1 and control virus. Human PDGF-BB or icatibant Hoe140, a BK B2 antagonistat, was used as the chemoattractant and placed in the bottom chamber of the Boyden chamber. The mRNA expressions of bradykinin B1 receptor and B2 receptor were detected by RT-PCR in VSMC(SHR).


RESULTS
hKLK1 gene transfer significantly inhibited PDGF-BB-induced migration of VSMC(SHR), with the peak inhibition rate of 34.6% (P<0.001). PDGF-BB significantly increased the mRNA expression of B2 receptor but not B1 receptor in VSMC(SHR).


CONCLUSIONS
hKLK1 gene transfer can inhibit the migration of VSMC(SHR) induced by PDGF-BB, and the inhibitory effects may be not mediated by bradykinin B2 receptor.
Yu
Hui-zhen
HZ
Fujian Provincial Institute of Hypertension, First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China. yhz200333@126.com
Xie
Liang-di
LD
Zhu
Peng-li
PL
Xu
Chang-sheng
CS
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D000077214:Becaplermin; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D006801:Humans; D006973:Hypertension / Q000473:pathology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D011918:Rats, Inbred SHR; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D020840:Tissue Kallikreins / Q000096:biosynthesis / Q000235:genetics*
D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D000077214:Becaplermin; D020840:Tissue Kallikreins