Anticancer activity of phenoxazines produced by bovine erythrocytes on colon cancer cells.
The present study investigated the anticancer activity of 2-aminophenoxazine-3-one (Phx-3) and 2-amino-4,4 alpha-dihydro-4 alpha,7-dimethyl-3H-phenoxazine-3-one (Phx-1), which were obtained by improved preparation methods using bovine erythrocyte suspension, on colon cancer cell lines COLO201, DLD1 and PMCO1 in vitro. The preparation methods for Phx-1 and Phx-3 had the advantages of extensively shortening reaction time and reducing sample volumes up to one-seventh during treatment, compared with the conventional method using bovine hemoglobin solution, resulting in extensive reduction of handling time. Phx-1 and Phx-3 thus obtained were identified as pure by the absorption spectra and NMR spectra. These phenoxazines exerted strong, dose-dependent anticancer activity against colon cancer cell lines COLO201, DLD1 and PMCO1 in vitro and induced apoptosis of these cells. The present results demonstrate that Phx-1 and Phx-3, which were prepared by extensively improved methods using bovine erythrocytes, may be useful as therapeutic drugs against colon cancer that is intractable to chemotherapy.
Nakachi
Takeshi
T
Fourth Department of Surgery, Tokyo Medical University, Ibaraki Medical Center, Ibaraki 300-0395, Japan.
Tabuchi
Takafumi
T
Takasaki
Akira
A
Arai
Sadao
S
Miyazawa
Keisuke
K
Tomoda
Akio
A
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects*; D002855:Chromatography, Thin Layer; D003110:Colonic Neoplasms / Q000188:drug therapy*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010078:Oxazines / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
C104202:2-amino-4,4alpha-dihydro-4alpha,7-dimethyl-3H-phenoxazine-3-one; D000970:Antineoplastic Agents; D010078:Oxazines; C005512:3-aminophenoxazone