High-stringency tandem affinity purification of proteins conjugated to ubiquitin-like moieties.
The post-translational modification of proteins with ubiquitin and ubiquitin-like proteins (Ubl) is vital to many cellular functions, and thus the identification of Ubl targets is key to understanding their function. In most cases, only a small proportion of the cellular pool of proteins is found conjugated to a particular Ubl, making identification of Ubl targets technically challenging. For the purposes of proteomic analyses, we have developed a protocol for the large-scale purification of Ubl-linked proteins that minimizes sample contamination with noncovalent interactors and prevents the cleavage of Ubl-substrate bonds catalyzed by Ubl-specific proteases. This is achieved by introducing a denaturing lysis step (in the presence of sodium dodecyl sulfate and alkylating agents that irreversibly inhibit Ubl proteases) before TAP (tandem affinity purification) that allows for efficient purification of putative Ubl-specific substrates in a form suitable for proteomic analysis. The timescale from cell lysis to purified protein sample is 5-6 d.
Golebiowski
Filip
F
Welcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, Scotland, UK. f.golebiowski@dundee.ac.uk
Tatham
Michael H
MH
Nakamura
Akihiro
A
Hay
Ronald T
RT
D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D006801:Humans; D011506:Proteins / Q000737:chemistry*; D014452:Ubiquitins / Q000737:chemistry*
D011506:Proteins; D014452:Ubiquitins