Multiplexed electrochemical DNA sensor for single-nucleotide polymorphism typing by using oligonucleotide-incorporated nonfouling surfaces.
In this work, we report a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection by using oligonucleotide-incorporated nonfouling surfaces (ONS). A typical "sandwich" scheme was employed to perform the SNP assay. The presence of the target DNA templated the ligation between the capture probe DNA anchored on gold electrodes and the tandem reporter probe tagged with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. This method could effectively differentiate SNP sites with only one-base mismatch. Importantly, the differentiation ratio was significantly higher than that with surfaces without the nonfouling property, which clearly demonstrated the superiority of the ONS strategy.
Wan
Ying
Y
Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China.
Lao
Ruojun
R
Liu
Gang
G
Song
Shiping
S
Wang
Lihua
L
Li
Di
D
Fan
Chunhai
C
D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide
D015342:DNA Probes; D006046:Gold; D004247:DNA; D010544:Peroxidases