Osteoblast response to commercially available demineralized bone matrices--an in-vitro study.
OBJECTIVE
Reconstruction of lost attachment apparatus is a major goal of periodontal therapy. Although various osteoinductive bone replacement grafts (BRGs) have been used with apparent clinical success, unequivocal evidence of osteoinductivity may be obtained only through the demonstration of increased osteoblastic/osteoclastic differentiation following exposure to these materials.


METHODS
Bone marrow stem cells (BMSCs) obtained from rat femur were cultured in Dulbecco's Modified Eagles Medium (DMEM) and 10% fetal bovine serum (FBS). They were then exposed to two demineralized bone matrices (DBM's)--Grafton and Osseograft, and divided into three groups, comprising of a negative control (BMSC + DMEM + 10% FBS), Grafton, Osseograft. An osteogenic medium (OM) (10 hm dexamethasone, 10 hm b-glycerophosphate, and 50 microg/ml ascorbic acid) was added to create three subgroups comprising of a positive control (OM), Grafton with OM, Osseograft with OM.


RESULTS
After an initial phase (up to day 5), both Grafton and Osseograft induced an increased proliferative activity in the BMSCs, which reached a plateau after day 10. These grafts also induced increased alkaline phosphatase activity when compared to the control groups and to BMSCs with an OM.


CONCLUSIONS
Both Osseograft and Grafton are capable of inducing osteoblastic proliferation and differentiation.
Kumaran
S Thanga
ST
Department of Periodontics, Ragas Dental College and Hospital, University of Madras, Taramani Campus, Chennai, India.
Arun
K V
KV
Sudarsan
Sabitha
S
Talwar
Avaneendra
A
Srinivasan
N
N
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D018786:Bone Substitutes / Q000494:pharmacology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen; D005269:Femur / Q000601:surgery; D005990:Glycerol / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D011509:Proteoglycans; D051381:Rats; D017208:Rats, Wistar
D018786:Bone Substitutes; C497176:Grafton demineralized bone matrix gel; D011509:Proteoglycans; D003094:Collagen; D000469:Alkaline Phosphatase; D005990:Glycerol