title: Nanoengineered electrically contacted enzymes on DNA scaffolds: functional assemblies for the selective analysis of Hg2+ ions.
abstract: A DNA construct consisting of a nucleic acid template, (1), on which a nucleic acid-modified glucose oxidase (GOx), (3), was hybridized by cooperative bridging of the T-Hg(2+)-T units, and a nucleic acid-functionalized ferrocene, (5), was directly hybridized on a Au electrode. The resulting nanostructure revealed bioelectrocatalytic activities, where the ferrocene units mediated electron transfer between the redox center of the enzyme and the electrode. The bioelectrocatalytic functions of the system are regulated by the concentration of Hg(2+) ions, which controls the content of the enzyme associated with the DNA template by means of the T-Hg(2+)-T bridging units. This phenomenon allowed the amperometric detection of Hg(2+) ions at a detection limit 1 x 10(-10) M with impressive selectivity.
authors.affiliation: Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors.forename: Gilad
authors.initials: G
authors.lastname: Mor-Piperberg
authors.forename: Ran
authors.initials: R
authors.lastname: Tel-Vered
authors.forename: Johann
authors.initials: J
authors.lastname: Elbaz
authors.forename: Itamar
authors.initials: I
authors.lastname: Willner
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004553:Electric Conductivity; D004563:Electrochemistry; D004566:Electrodes; D004738:Engineering; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D008628:Mercury / Q000032:analysis*; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization
chemical_list: D004800:Enzymes, Immobilized; D004247:DNA; D005949:Glucose Oxidase; D008628:Mercury