Determination of p-aminohippuric acid in rat plasma by liquid chromatography-tandem mass spectrometry.
A rapid, simple and sensitive method was developed for the determination of para-aminohippuric acid (PAH) in rat plasma using liquid chromatography tandem mass spectrometry (LC-MS-MS). Acetaminophen was used as the internal standard. Chromatographic separation was performed using a Symmetry C18 column and the mobile phase was composed of A: 2 mM ammonium formate and 0.1% formic acid in water and B: 2 mM ammonium formate and 0.1% formic acid in acetonitrile (ACN) (A:B, 30:70, v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer using positive ion mode electrospray ionization (ESI) in the multiple reaction monitoring (MRM) mode. The MS/MS ion transitions monitored were m/z 195.2-->120.2 and 152.1-->110.1 for PAH and acetaminophen, respectively. Good linearity is observed over the concentration range of 0.1-500 microg/ml. The method was proved to be accurate and reliable and was applied to a pharmacokinetic study in rat.
Fan
Hsien-Yuan
HY
School of Pharmacy, National Defense Medical Center, Taipei, Taiwan, ROC.
Lin
Chang-Ching
CC
Pao
Li-Heng
LH
D000082:Acetaminophen / Q000032:analysis; D000818:Animals; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D016014:Linear Models; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D010130:p-Aminohippuric Acid / Q000097:blood* / Q000493:pharmacokinetics
D000082:Acetaminophen; D010130:p-Aminohippuric Acid