Proteomic analysis in canine leishmaniasis.
The aim of this study was to perform a proteomic analysis on serum of dogs naturally infected with Leishmania parasite. Sera from 24 dogs, n. 8 with high IFAT titre of anti-Leishmania antibodies (>or= 1:640), n. 8 with uncertain titre (= 1:40), and n. 8 with IFAT negative were used. Sera of each group were pooled together to form three pools: P (high titre); U (uncertain titre); and N (negative). The P pool was analyzed, using a mass spectrometry-based approach to search for Leishmania proteins (qualitative analysis). In a second experiment, protein signal intensities of U and P pools were compared with the signal intensities of N pool by a quantitative mass spectrometry method based on isotopic dilution. The quantitative analysis detected a total of 70 proteins, of which 17 and 5 resulted over- and under-represented in sample P, respectively.
Britti
D
D
Dipartimento Medicina Sperimentale e Clinica Università degli Studi di Catanzaro Magna Graecia, Catanzaro, Italy. britti@unicz.it
Gaspari
M
M
Massimini
G
G
Casalinuovo
F
F
Morittu
V M
VM
Cuda
G
G
D000818:Animals; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D020869:Gene Expression Profiling / Q000662:veterinary*; D005786:Gene Expression Regulation; D007896:Leishmaniasis / Q000378:metabolism*; D008297:Male; D040901:Proteomics