title: Motility enhancement of bacteria actuated microstructures using selective bacteria adhesion.
abstract: Microrobots developed by the technological advances are useful for application in various fields. Nevertheless, they have limitations with respect to their actuator and motility. Our experiments aim to determine whether a bioactuator using the flagellated bacteria Serratia marcescens would enhance the motility of microrobots. In this study, we investigate that the flagellated bacteria Serratia marcescens could be utilized as actuators for SU-8 microstructures by bovine serum albumin-selective patterning. Firstly, we analyze the adherence of the bacteria to the SU-8 micro cube by selective patterning using 5% BSA. The results show that number of attached-bacteria in the uncoated side of the selectively- coated micro cube with BSA increased by 200% compared with that in all sides of the non treated micro cube. Secondly, the selectively BSA coated micro cube had 210% higher motility than the uncoated micro cube. The results revealed that the bacteria patterned to a specific site using 5% BSA significantly increase the motility of the bacteria actuated microstructure.
authors.affiliation: School of Mechanical Systems Engineering, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Sung Jun
authors.initials: SJ
authors.lastname: Park
authors.forename: Hyeoni
authors.initials: H
authors.lastname: Bae
authors.forename: Joonhwuy
authors.initials: J
authors.lastname: Kim
authors.forename: Byungjik
authors.initials: B
authors.lastname: Lim
authors.forename: Jongoh
authors.initials: J
authors.lastname: Park
authors.forename: Sukho
authors.initials: S
authors.lastname: Park
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002633:Chemotaxis / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D012371:Robotics / Q000295:instrumentation*; D012706:Serratia marcescens / Q000166:cytology / Q000502:physiology*