title: [Breeding of ammonium-tolerant mutants of Actinobacillus succinogenes for succinic acid production and effect of ammonium].
abstract: An ammonium-tolerant mutant of Actinobacillus succinogenes, YZ25, was obtained in the medium containing 61-242 mmol/L NH4+ after DES mutagenesis. Succinic acid produced by the mutant YZ25 reached 32.68 g/L when the medium contains 50 g/L glucose and 121 mmol/L ammonium, which was increased by 180.5% compared with that of the parent strain. The effects of different ammonium salts on the growth of the mutant and its metabolic response to high ammonium concentrations were investigated. The results showed that low ammonium concentration could improve the specific growth rates of the mutants, while high ammonium concentration inhibited cell growth. The ammonia-nitrogen half-inhibition constants (Ki) for different ammonium salts were as follows: 215 mmol/L for (NH4)2SO4, 265 mmol/L for NH4HCO3, 235 mmol/L for NH4Cl, and 210 mmol/L for NH4NO3. The process of ammonium inhibition on the mutant YZ25 was investigated in 3.0 L stirred fermenter. When NH4OH was used to buffer the pH, cell growth was not inhibited. However, production of succinic acid and consumption of glucose gradually decreased when cells entered the stationary phase, and the glucose could not be utilized completely at the end of fermentation. The possible ammonium inhibition mechanism was discussed based on the metabolic pathway of A. succinogenes.
authors.affiliation: State Key Laboratory of Materials-oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, China.
authors.forename: Guizi
authors.initials: G
authors.lastname: Ye
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Kequan
authors.initials: K
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Yonglan
authors.initials: Y
authors.lastname: Xi
authors.forename: Xiumei
authors.initials: X
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
mesh_terms: D000188:Actinobacillus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019149:Bioreactors; D004361:Drug Tolerance; D005285:Fermentation; D007218:Industrial Microbiology; D009154:Mutation; D000644:Quaternary Ammonium Compounds / Q000378:metabolism / Q000494:pharmacology*; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D000644:Quaternary Ammonium Compounds; D019802:Succinic Acid