title: bla(CTX-M-2) and bla(CTX-M-28) extended-spectrum beta-lactamase genes and class 1 integrons in clinical isolates of Klebsiella pneumoniae from Brazil.
abstract: Twenty-eight Klebsiella pneumoniae clinical isolates that exhibited an extended-spectrum cephalosporin-resistance profile from a city in the Northeast of Brazil were analysed by PCR and DNA sequencing in order to determine the occurrence of blaCTX-M genes and class 1 integrons. We determined the occurrence of the blaCTX-M-2 gene in six K. pneumoniae isolates and describe the first detection of the blaCTX-M-28 gene in South America. Seven isolates carried class 1 integrons. Partial sequencing analysis of the 5'-3'CS variable region in the class 1 integrons of three isolates revealed the presence of aadA1, blaOXA-2 and dfr22 gene cassettes.
authors.affiliation: Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, Brasil. ana.lopes.ufpe@gmail.com
authors.forename: Ana Catarina S
authors.initials: AC
authors.lastname: Lopes
authors.forename: Dyana Leal
authors.initials: DL
authors.lastname: Veras
authors.forename: Alexsandra Mariá S
authors.initials: AM
authors.lastname: Lima
authors.forename: Rita de Cássia Andrade
authors.initials: Rde C
authors.lastname: Melo
authors.forename: Juan
authors.initials: J
authors.lastname: Ayala
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D032023:Integrons / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C071167:beta-lactamase CTX-2; C544678:CTX-M-28 beta-lactamase, Klebsiella pneumoniae; D001618:beta-Lactamases