[Correction of oxidative stress in the rat brain cortical cellular culture with vitamines E and C].
The oxidative stress-induced alteration in concentration of oxygen, lipid and nitrogen free radicals in cultured brain cortex of the newborn albino rats and its correction with vitamins E and C was investigated. Modeling the oxidative stress was achieved with addition of H(2)O(2) into the nutrient medium. In order to prevent an oxidative stress-induced cytotoxic effect, concomitantly with H(2)O(2), vitamins E and C were added into the nutrient medium. Oxygen, lipid and nitrogen free radicals were registered by method Electron Paramagnetic Resonance (EPR) and spin-traps. The data, obtained in our study revealed decreasing of intensity of NO content and increasing of spin trapped superoxid- (O(2-)) and lipoperoxid-radicals (LOO.) in explants of brain cortex of the newborn albino rats cultured in oxidative stress conditions. These changes were attenuated following action of vitamins E and C (decreasing of intensity of EPR signals of O2- and LOO, increasing of free NO content).
Davitashvili
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Museridze
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Svanidze
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Pavliashvili
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Sanikidze
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D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000494:pharmacology*; D014815:Vitamins / Q000494:pharmacology*
D000975:Antioxidants; D014815:Vitamins; D014810:Vitamin E; D001205:Ascorbic Acid