title: [Kallikrein-binding protein promotes axonal regeneration and protect rat retinal ganglion cells following optic nerve injury].
abstract: OBJECTIVE
  To explore the effect of kallikrein-binding protein (KPB) in protecting retinal ganglion cells (RGCs) and promoting axonal regeneration following optical nerve injury in rats.
  
  
  METHODS
  Crush injury of the optic nerve at 0.5-1.0 mm from the eyeball was induced in rats, which received subsequent KBP injection into the vitreous cavity (experimental group) and PBS injection (control group). At 7, 14 and 21 days after the injury, the rats were sacrificed and frozen sections of the eyeball were prepared to observe the structure and thickness of the retina and count the number of survival RGCs with HE staining. The optic nerves were collected for Western blotting to assess the effect of KBP on the RGCs and axonal regeneration.
  
  
  RESULTS
  RGC counts and retinal thickness showed significant differences between the two groups. Western blotting also demonstrated a significant difference in the expression of the nerve regeneration marker protein GAP-43 between the two groups.
  
  
  CONCLUSIONS
  KBP offers protection on RGCs and promotes regeneration of the optic nerve axons after optic nerve injury in rats.
authors.affiliation: Department of Ophthalmology, Kunming Children's Hospital, Kunming Medical College, Kunming 650031, China.
authors.forename: Shun-xiang
authors.initials: SX
authors.lastname: Jin
authors.forename: Yong
authors.initials: Y
authors.lastname: Zeng
authors.forename: Jing
authors.initials: J
authors.lastname: Wan
authors.forename: Kun
authors.initials: K
authors.lastname: Wan
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Li
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Li
authors.forename: Ting-hua
authors.initials: TH
authors.lastname: Wang
authors.forename: Zhong-tang
authors.initials: ZT
authors.lastname: Feng
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020221:Optic Nerve Injuries / Q000188:drug therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000502:physiology; D015843:Serpins / Q000494:pharmacology*
chemical_list: D019922:GAP-43 Protein; D018696:Neuroprotective Agents; D015843:Serpins; C051324:kallistatin