title: The antiallergic effects and acute toxicity of Lactobacillus crispatus KT-11 cultured in food grade medium.
abstract: We synthesized a medium consisting of commercial food supplements (food grade medium) that could be used to cultivate Lactobacillus crispatus KT-11 (KT-11), and investigated the antiallergic effects and acute toxicity of KT-11 cultured in this medium. We found that the growth of KT-11 in the food grade medium was comparable to that in DeMan-Rogosa-Sharpe (MRS) medium. Sneezing event was reduced in ovalbumin (OVA)-sensitized BALB/c mice given a diet supplemented with KT-11 grown in the food grade medium (FG-KT-11 group) when compared to mice given a diet supplemented with KT-11 grown in MRS medium (MRS-KT-11 group). The number of CD80(+)CD11b(+) Peyer's patch cells was significantly lower in the FG-KT-11 group than in the MRS-KT-11 group, while IL-12(+)CD11b(+) Peyer's patch cells were higher in the FG-KT-11 group. Only minimal acute toxicity was observed in ICR mice given 1000 or 2000 mg of FG-KT-11/kg body weight. These results suggest that FG-KT-11 represents a safe antiallergic food material.
authors.affiliation: Interdisciplinary Graduate School of Science and Technology, Shinshu University, Minamiminowa-mura 8304, Kamiina-gun, Nagano 399-4598, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Tobita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yanaka
authors.forename: Hajime
authors.initials: H
authors.lastname: Otani
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage*; D018122:B7-1 Antigen / Q000032:analysis; D039481:CD11b Antigen / Q000032:analysis; D002452:Cell Count; D003470:Culture Media; D019587:Dietary Supplements / Q000633:toxicity*; D005260:Female; D006967:Hypersensitivity / Q000473:pathology / Q000517:prevention & control*; D018664:Interleukin-12 / Q000032:analysis; D007778:Lactobacillus / Q000254:growth & development* / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009929:Organ Size; D010047:Ovalbumin / Q000276:immunology; D010581:Peyer's Patches / Q000276:immunology / Q000473:pathology
chemical_list: D018926:Anti-Allergic Agents; D018122:B7-1 Antigen; D039481:CD11b Antigen; D003470:Culture Media; D018664:Interleukin-12; D010047:Ovalbumin