Induction of indoleamine 2,3-dioxygenase expression via heme oxygenase-1-dependant pathway during murine dendritic cell maturation.
Heme oxygenase (HO)-1 is expressed in a variety of conditions involved in the regulation of immune responses. In this study, we examined the role of HO-1 in dendritic cell (DC) maturation and expression of indoleamine 2,3-dioxygenase (IDO), a key enzyme that catalyzes the initial, rate-limiting step in tryptophan degradation. IDO deficiency led to diminished phenotypic and functional maturation of DCs in vitro and in vivo. IDO expression and DC maturation was abrogated by the HO inhibitor zinc protoporphrin, but increased by hemin, a potent inducer of HO-1. Moreover, LPS-induced HO-1 expression was mediated by an NF-kappaB-dependent pathway. Our findings provide additional insight into the immunological functions of IDO and HO-1, and suggest possible therapeutic adjuvants for the treatment of DC-related acute and chronic diseases.
Jung
In Duk
ID
Department of Microbiology, Pusan National University, Beom-eo Ri, Mulgum Eop, Yangsan, Gyeongsangnam-do, South Korea.
Lee
Jun Sik
JS
Lee
Chang-Min
CM
Noh
Kyung Tae
KT
Jeong
Yeong-Il
YI
Park
Won Sun
WS
Chun
Sung Hak
SH
Jeong
Soo Kyung
SK
Park
Jin Wook
JW
Son
Kwang Hee
KH
Heo
Deok Rim
DR
Lee
Min-Goo
MG
Shin
Yong Kyoo
YK
Kim
Han Wool
HW
Yun
Cheol-Heui
CH
Park
Yeong-Min
YM
D000818:Animals; D048708:Cell Growth Processes; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D003713:Dendritic Cells / Q000502:physiology*; D004790:Enzyme Induction; D051547:Heme Oxygenase-1 / Q000502:physiology*; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL
D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D051547:Heme Oxygenase-1