Discrimination of cyanobacterial strains isolated from saline soils in Nakhon Ratchasima, Thailand using attenuated total reflectance FTIR spectroscopy.
A method was developed whereby high quality FTIR spectra could be rapidly acquired from soil-borne filamentous cyanobacteria using ATR FTIR spectroscopy. Spectra of all strains displayed bands typical of those previously reported for microalgae and water-borne cyanobacteria, with each strain having a unique spectral profile. Most variation between strains occurred in the C-O stretching and the amide regions. Soft Independent Modelling by Class Analogy (SIMCA) was used to classify the strains with an accuracy of better than 93%, with best classification results using the spectral region from 1800-950 cm(-1). Despite this spectral region undergoing substantial changes, particularly in amide and C-O stretching bands, as cultures progressed through the early-, mid- to late-exponential growth phases, classification accuracy was still good (approximately 80%) with data from all growth phases combined. These results indicate that ATR/FTIR spectroscopy combined with chemometric classification methods constitute a rapid, reproducible, and potentially automated approach to classifying soil-borne filamentous cyanobacteria.
Bounphanmy
Somchanh
S
School of Biology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
Thammathaworn
Sompong
S
Thanee
Nathawut
N
Pirapathrungsuriya
Komson
K
Beardall
John
J
McNaughton
Don
D
Heraud
Philip
P
D000465:Algorithms; D015373:Bacterial Typing Techniques / Q000379:methods*; D000458:Cyanobacteria / Q000145:classification*; D054712:Salinity; D012988:Soil Microbiology; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013785:Thailand