Human umbilical cord blood mesenchymal stem cell-derived extracellular matrix prohibits metastatic cancer cell MDA-MB-231 proliferation.
It is not clear whether adult stem cell extracellular matrix (ECM) can regulate cancer cells. We demonstrated that the ECM produced by UCB-MSCs was able to arrest the growth of metastatic tumor cells by upregulating levels of PTEN in aggressive cancer cells. Human UCB-MSCs produced dickkopf (DKK1) are capable of inhibiting cancer cell proliferation but has no contribution to the tumor inhibition effect of UCB-MSC ECM. This study also provides an innovative approach to specifically examine the effect of stem cell microenvironments on cancer cells without the complexity of cell-cell interactions. In conclusion, human UCB-MSC ECM prohibits cancer cell proliferation.
Sun
Bo
B
Adult Stem Cell Research Center, Seoul National University, South Korea.
Yu
Kyung-Rok
KR
Bhandari
Dilli Ram
DR
Jung
Ji-Won
JW
Kang
Soo-Kyung
SK
Kang
Kyung-Sun
KS
D000328:Adult; D000818:Animals; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000187:drug effects / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D016685:Mitomycin / Q000494:pharmacology; D009362:Neoplasm Metastasis / Q000473:pathology / Q000517:prevention & control*; D051059:PTEN Phosphohydrolase / Q000235:genetics; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction
C501126:DKK1 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D049452:Green Fluorescent Proteins; D016685:Mitomycin; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human