title: Enantioseparation of amino acids and alpha-hydroxy acids on ligand-exchange continuous beds by capillary electrochromatography.
abstract: A new chiral stationary phase based on continuous bed (CB) technology using L-prolinamide as a chiral selector was prepared. Its ability for enantioseparation of amino acids and alpha-hydroxy acids by ligand-exchange CEC was compared with that of a CB containing L-4-hydroxyproline as chiral selector. Preparation was done by a one-step in situ copolyermerization procedure using methacrylamide as monomer, vinylsulfonic acid as charge providing agent and piperazine diacrylamide as crosslinker. L-Prolinamide showed marked enantioselective properties for the separation of amino acids and alpha-hydroxy acids. Furthermore, we show the possibility of simultaneous enantioseparation of amino acids and alpha-hydroxy acids using the CB with L-4-hydroxyproline as chiral selector.
authors.affiliation: Laboratory of Flow Analysis and Chromatography, Department of Chemistry, Warsaw University, Warsaw, Poland.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Puchalska
authors.forename: Elfriede
authors.initials: E
authors.lastname: Pittler
authors.forename: Marek
authors.initials: M
authors.lastname: Trojanowicz
authors.forename: Gerald
authors.initials: G
authors.lastname: Gübitz
authors.forename: Martin G
authors.initials: MG
authors.lastname: Schmid
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D053801:Capillary Electrochromatography / Q000379:methods*; D004295:Dihydroxyphenylalanine / Q000737:chemistry / Q000302:isolation & purification; D006880:Hydroxy Acids / Q000737:chemistry / Q000302:isolation & purification*; D006909:Hydroxyproline / Q000737:chemistry / Q000302:isolation & purification; D007773:Lactates / Q000737:chemistry / Q000302:isolation & purification; D010649:Phenylalanine / Q000737:chemistry / Q000302:isolation & purification; D011392:Proline / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D006880:Hydroxy Acids; D007773:Lactates; C017648:3-phenyllactic acid; D010649:Phenylalanine; D004295:Dihydroxyphenylalanine; D011392:Proline; D006909:Hydroxyproline; C056291:prolinamide