Toxoplasma gondii: flat-mounting of retina as a new tool for the observation of ocular infection in mice.
Ocular toxoplasmosis is the principal cause of posterior uveitis and a leading cause of blindness. Animal models are required to improve our understanding of the pathogenesis of this disease. The method currently used for the detection of retinal cysts in animals involves the observation, under a microscope, of all the sections from infected eyes. However, this method is time-consuming and lacks sensitivity. We have developed a rapid, sensitive method for observing retinal cysts in mice infected with Toxoplasma gondii. This method involves combining the flat-mounting of retina - a compromise between macroscopic observation and global analysis of this tissue - and the use of an avirulent recombinant strain of T. gondii expressing the Escherichia coli beta-galactosidase gene, visually detectable at the submacroscopic level. Single cyst unilateral infection was found in six out of 17 mice killed within 28 days of infection, whereas a bilateral infection was found in only one mouse. There was no correlation between brain cysts number and ocular infection.
Escoffier
P
P
Université Pierre et Marie Curie-Paris6, UMR S 945, Paris F-75013, France. patricia.escoffier@gmail.com
Jeanny
J C
JC
Marinach-Patrice
C
C
Jonet
L
L
Raoul
W
W
Behar-Cohen
F
F
Paris
L
L
Danis
M
M
Dubremetz
J F
JF
Mazier
D
D
D000818:Animals; D001921:Brain / Q000469:parasitology; D004195:Disease Models, Animal; D005260:Female; D005629:Frozen Sections; D051379:Mice; D012160:Retina / Q000469:parasitology*; D014122:Toxoplasma / Q000302:isolation & purification*; D014126:Toxoplasmosis, Ocular / Q000175:diagnosis / Q000469:parasitology*