title: Induction of DNA breakage in U937 cells by oxazaphosphorines.
abstract: Oxazaphosphorines are a class of DNA alkylating agents. The aim of the present study was to compare the possible influence of three new generation oxazaphosphorines, D-17272 (mafosfamide cyclohexylamine salt), D-18864 (4-hydro-peroxy-cyclophosphamide), and D-19575 (glufosfamide, beta-D-glucose-isophosphoramide mustard) on DNA damage induction in the human histiocytic lymphoma U937 cells. The flow cytometry APO-BRDU assay, based on the TUNEL method, was used for the in situ detection of DNA strand breaks. After exposure of U937 cells to the oxazaphosphorines, the patterns of temporary changes in the frequency of TUNEL positive U937 cells, expressing DNA breakage, were determined. The effects of the oxazaphosphorines on U937 cells were dependent on the agent tested and its dose, and the time intervals after the drug application. The different potential of D-17272, D-18864 and D-19575 to induce DNA strand breakage in the human histiocytic lymphoma U937 cells was shown.
authors.affiliation: Department of Experimental Hematology, Jagiellonian University, R. Ingardena 6, 30-060 Kraków, Poland. lidia.mazur@uj.edu.pl
authors.forename: Lidia
authors.initials: L
authors.lastname: Mazur
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Opydo-Chanek
authors.forename: Marta
authors.initials: M
authors.lastname: Stojak
authors.forename: Jarosław
authors.initials: J
authors.lastname: Baran
authors.forename: Ulf
authors.initials: U
authors.lastname: Niemeyer
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000494:pharmacology; D053960:DNA Breaks / Q000187:drug effects*; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D007069:Ifosfamide / Q000031:analogs & derivatives* / Q000494:pharmacology; D010752:Phosphoramide Mustards / Q000494:pharmacology*; D020298:U937 Cells
chemical_list: D018906:Antineoplastic Agents, Alkylating; D010752:Phosphoramide Mustards; C092028:beta-D-glucosylisophosphoramide mustard; C037933:Asta Z 7557; D003520:Cyclophosphamide; D005947:Glucose; D007069:Ifosfamide