Prevalence and antimicrobial resistance of Salmonella serotypes in chickens from retail markets in Yaounde (Cameroon).
From February 2006 to January 2007, 150 chickens were collected from eight retail markets in Yaounde, and 90 (60%) tested positive for Salmonella. Seventy-nine chickens were contaminated with only one Salmonella serotype, 10 with two different serotypes, and 1 with four serotypes. The most prevalent serotypes were Enteritidis (47 strains) and Hadar (29 strains). The isolates were tested for their susceptibilities to amoxicillin, amoxicillin/clavulanic acid, cefoxitin, cefotaxime, gentamicin, streptomycin, tetracycline, chloramphenicol, sulfonamides, nalidixic acid, ciprofloxacin, and trimethoprim/sulfamethazole by disk diffusion assay. Minimum inhibitory concentrations of ampicillin, streptomycin, tetracycline, sulfonamides, and nalidixic acid were determined for the resistant strains by agar dilution method. Eleven isolates (10.7%) of the 103 tested were susceptible to all antimicrobials. Resistance was most observed to tetracycline (84.5%), streptomycin (44.7%), and nalidixic acid (34%). Forty-one isolates (39.8%) were multidrug resistant (resistant to three or more antimicrobials from different classes), of which 68.3% were Hadar and 21.9% Enteritidis. The most frequent resistant pattern in Hadar was streptomycin-tetracycline-nalidixic acid. These results highlight once more the need for surveillance of Salmonella contamination in poultry.
Wouafo
Marguerite
M
Laboratory of Bacteriology, Pasteur Centre of Cameroon, Yaounde, Cameroon.
Nzouankeu
Ariane
A
Kinfack
Junie Atangana
JA
Fonkoua
Marie-Christine
MC
Ejenguele
Guy
G
Njine
Thomas
T
Ngandjio
Antoinette
A
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002163:Cameroon; D002645:Chickens / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005506:Food Contamination; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D012475:Salmonella / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D012703:Serotyping
D000900:Anti-Bacterial Agents