title: Modulation of blackspot seabream (Pagellus bogaraveo) intermediary metabolic pathways by dispensable amino acids.
abstract: The objective of the present work is to investigate the main metabolic pathways by which dispensable amino acids (DAA) are diverted towards lipid formation in blackspot seabream. For that purpose, a control diet was formulated to contain 45% of crude protein (7.2 g N/100 g dry matter) mainly supplied by fish meal (45P). In two other diets, 22.2% of the dietary nitrogen (1.6 g N/100 g dry matter) was replaced by an equivalent amount of nitrogen provided by two different mixtures of DAA: alanine and serine (diet AS) or aspartic and glutamic acid (diet AG). A fourth diet (diet 35P) only containing 35% of crude protein (5.6 g N/100 g dry matter) was included in order to analyze the possible additive effects of DAA. Compared to fish fed diet 35P, blackspot seabream appear to make a more efficient use of the nitrogen provided by alanine and serine than that provided by aspartic and glutamic acids in terms of growth. Contrary to fish fed AG, fish fed AS attained similar specific FAS activities as 45P fed fish, suggesting a further role of alanine and serine on this lipogenic pathway. Dietary nitrogen reduction (45P vs. 35P) or its replacement by a mixture of aspartic and glutamic acids (diet AG) were shown to up-regulate phosphoenolpyruvate carboxykinase (PEPCK) but without, however, any effect on plasma glucose levels. Dietary nitrogen level and nature seems to exert a complex regulation on energetic pathways through the gluconeogenesis/tricarboxylic acids cycle interaction.
authors.affiliation: CIMAR/CIIMAR-Centro Interdisciplinar de Investigação Marinha e Ambiental, and ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua dos Bragas, 289, 4050-123, Porto, Portugal.
authors.forename: A Cláudia
authors.initials: AC
authors.lastname: Figueiredo-Silva
authors.forename: Geneviève
authors.initials: G
authors.lastname: Corraze
authors.forename: Sadasivam
authors.initials: S
authors.lastname: Kaushik
authors.forename: José B
authors.initials: JB
authors.lastname: Peleteiro
authors.forename: Luísa M P
authors.initials: LM
authors.lastname: Valente
mesh_terms: D000596:Amino Acids / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage / Q000378:metabolism; D021541:Sea Bream / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D004044:Dietary Proteins