OsZIP5 is a plasma membrane zinc transporter in rice.
Zinc is essential for normal plant growth and development. To understand its transport in rice, we characterized OsZIP5, which is inducible under Zn deficiency. OsZIP5 complemented the growth defect of a yeast Zn-uptake mutant, indicating that OsZIP5 is a Zn transporter. The OsZIP5-GFP fusion protein was localized to the plasma membrane. Transgenic plants overexpressing the gene grew less well. Overexpression of the gene decreased the Zn concentration in shoots, but caused it to rise in the roots. Knockout plants showed no visible phenotypic changes under either normal or deficient conditions. However, they were tolerant to excess Zn and contained less Zn. In contrast, overexpressing transgenics were sensitive to excess Zn. These results indicate that OsZIP5 plays a role in Zn distribution within rice.
Lee
Sichul
S
Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, Republic of Korea.
Jeong
Hee Joong
HJ
Kim
Sun A
SA
Lee
Joohyun
J
Guerinot
Mary Lou
ML
An
Gynheung
G
D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005816:Genetic Complementation Test; D000090463:Iron Deficiencies; D016254:Mutagenesis, Insertional / Q000187:drug effects / Q000235:genetics; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified; D021381:Protein Transport / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D012639:Seeds / Q000187:drug effects / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D015032:Zinc / Q000172:deficiency / Q000378:metabolism / Q000494:pharmacology
D002352:Carrier Proteins; D004269:DNA, Bacterial; D010940:Plant Proteins; C036483:T-DNA; C017800:zinc-binding protein; D015032:Zinc