title: Bacterially expressed double-stranded RNAs against hot-spot sequences of tobacco mosaic virus or potato virus Y genome have different ability to protect tobacco from viral infection.
abstract: Posttranscriptional gene silencing, also known as RNA interference, involves degradation of homologous mRNA sequences in organisms. In plants, posttranscriptional gene silencing is part of a defense mechanism against virus infection, and double-stranded RNA is the pivotal factor that induces gene silencing. In this paper, we got seven hairpin RNAs (hpRNAs) constructs against different hot-spot sequences of Tobacco mosaic virus (TMV) or Potato virus Y (PVY) genome. After expression in Escherichia coli HT115, we extracted the seven hpRNAs for the test in tobacco against TMV or PVY infection. The data suggest that different hpRNAs against different hot-spot sequences of TMV or PVY genome had different ability to protect tobacco plants from viral infection. The resistance to TMV conferred by the hpRNA against the TMV movement protein was stronger than other TMV hpRNAs; the resistance to PVY conferred by the hpRNA against the PVY nuclear inclusion b was better than that induced by any other PVY hpRNAs. Northern blotting of siRNA showed that the resistance was indeed an RNA-mediated virus resistance.
authors.affiliation: Shandong Agricultural University, Tai'an, China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Yin
authors.forename: Zhao-Nan
authors.initials: ZN
authors.lastname: Sun
authors.forename: Yun-Zhi
authors.initials: YZ
authors.lastname: Song
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: An
authors.forename: Chang-Xiang
authors.initials: CX
authors.lastname: Zhu
authors.forename: Fu-Jiang
authors.initials: FJ
authors.lastname: Wen
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D055029:Inverted Repeat Sequences; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral