Mice cloned from induced pluripotent stem cells (iPSCs).
Differentiated somatic cells of various species can be reprogrammed into induced pluripotent stem cells (iPSCs) by ectopically expressing a combination of several transcription factors that are highly enriched in embryonic stem cells (ESCs). The generation of iPSCs in large animals has raised the possibility of producing genetically modified large animals through the nuclear transplantation approach. However, it remains unknown whether iPSCs could be used for generating cloned animals through the nuclear transfer method. Here, we show the successful production of viable cloned mice from inducible iPSCs through the nuclear transfer approach, and the efficiency is similar to that of using ESCs derived via normal fertilization. Furthermore, the cloned mice are fertile and can produce second-generation offspring. These efforts strengthen the possibility of utilizing iPSCs to generate gene-modified large animals for pharmaceutical purposes in the future.
Kou
Zhaohui
Z
National Institute of Biological Sciences, NIBS, Beijing, China.
Kang
Lan
L
Yuan
Ye
Y
Tao
Yu
Y
Zhang
Yu
Y
Wu
Tong
T
He
Jing
J
Wang
Jianle
J
Liu
Zhonghua
Z
Gao
Shaorong
S
D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics; D019976:Cloning, Organism / Q000379:methods*; D047108:Embryonic Development; D053595:Embryonic Stem Cells; D005260:Female; D005298:Fertility; D015870:Gene Expression; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D053652:Nuclear Transfer Techniques; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000648:ultrastructure*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014162:Transfection
D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D050814:Octamer Transcription Factor-3; D016271:Proto-Oncogene Proteins c-myc; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors