title: Determination of chromones in Dysophylla stellata by HPLC: method development, validation and comparison of different extraction methods.
abstract: An HPLC method with reflux as an efficient extraction method has been developed for quantification of chromones in Dysophylla stellata Benth. Separation was achieved on a C18 column with a mobile phase of 0.5% (v/v) acetic acid in water (A), and ACN (B) under gradient elution at 1 mL/min. Chromones (1 and 2) isolated from D. stellata were used as standards for method development and validation was achieved according to ICH guidelines. Extracts prepared by three different methods [reflux, ultrasonication and accelerated solvent extraction (ASE)] were used for analysis by the validated method. The proposed HPLC method is simple, accurate and selective for the separation and quantification of chromones in D. stellata.
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, Mohali (Punjab), India.
authors.forename: Raju
authors.initials: R
authors.lastname: Gautam
authors.forename: Amit
authors.initials: A
authors.lastname: Srivastava
authors.forename: Sanjay M
authors.initials: SM
authors.lastname: Jachak
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002867:Chromones / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D019686:Lamiaceae / Q000737:chemistry*; D057230:Limit of Detection; D015203:Reproducibility of Results
chemical_list: D002867:Chromones