Single nucleotide polymorphisms in the FADS gene cluster are associated with delta-5 and delta-6 desaturase activities estimated by serum fatty acid ratios.
Genetic variability in the FADS1-FADS2 gene cluster [encoding delta-5 (D5D) and delta-6 (D6D) desaturases] has been associated with plasma long-chain PUFA (LCPUFA) and lipid levels in adults. To better understand these relationships, we further characterized the association between FADS1-FADS2 genetic variability and D5D and D6D activities in adolescents. Thirteen single nucleotide polymorphisms (SNPs) were genotyped in 1,144 European adolescents (mean +/- SD age: 14.7 +/- 1.4 y). Serum phospholipid fatty acid levels were analyzed using gas chromatography. D5D and D6D activities were estimated from the C20:4n-6/C20:3n-6 and C20:3n-6/C18:2n-6 ratios, respectively. Minor alleles of nine SNPs were associated with higher 18:2n-6 levels (1.9E-18 <or= P <or= 6.1E-5), lower C20:4n-6 levels (7.1E-69 <or= P <or= 1.2E-12), and lower D5D activity (7.2E-44 <or= P <or= 4.4E-5). All haplotypes carrying the rs174546 minor allele were associated with lower D5D activity, suggesting that this SNP is in linkage disequilibrium with a functional SNP within FADS1. In contrast, only the rs968567 minor allele was associated with higher D6D activity (P = 1.5E-6). This finding agrees with an earlier in vitro study showing that the minor allele of rs968567 is associated with a higher FADS2 promoter activity. These results suggest that rare alleles of several SNPs in the FADS gene cluster are associated with higher D6D activity and lower D5D activity in European adolescents.
Bokor
Szilvia
S
Inserm U744, Institut Pasteur de Lille, Univ Lille Nord de France, UDSL, 59019 Lille, France.
Dumont
Julie
J
Spinneker
Andre
A
Gonzalez-Gross
Marcela
M
Nova
Esther
E
Widhalm
Kurt
K
Moschonis
George
G
Stehle
Peter
P
Amouyel
Philippe
P
De Henauw
Stefaan
S
Molnàr
Dènes
D
Moreno
Luis A
LA
Meirhaeghe
Aline
A
Dallongeville
Jean
J
D000293:Adolescent; D015992:Body Mass Index; D000090943:Delta-5 Fatty Acid Desaturase; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism*; D005227:Fatty Acids / Q000097:blood* / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000097:blood / Q000737:chemistry; D005260:Female; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D005810:Multigene Family / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
D000090943:Delta-5 Fatty Acid Desaturase; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D044943:Fatty Acid Desaturases; C000716847:FADS1 protein, human; C533241:FADS2 protein, human