Chemical and crystallographic study of remineralized surface on initial carious enamel treated with Galla chinensis.
To investigate the morphologic, chemical and crystallographic characters of remineralized surface on initial carious enamel treated with Galla chinensis, scanning electron microscopy equipped with energy dispersive analysis spectroscopy were used, and X-ray microdiffraction (microzone XRD) was used for the first time to analyze in situ the microzone crystallite of remineralized surface on carious enamel. Bovine sound enamel slabs were demineralized to produce initial carious lesion in vitro. Then, the lesions were exposed to a pH-cycling regime for 12 days of remineralization. Each daily cycle included 4x1 min applications with one of the three treatments: distilled and deionized water (DDW); 1 g/L NaF; 4 g/L G. chinensis extract (GCE). After the treatments, some rod-like deposits and many irregular prominences were found on GCE-treated enamel surface, and the intensities of Ca and P signals showed a tendency to increase; Ca:P ratio was significantly higher than that of DDW-treated enamel. X-ray microdiffraction showed hydroxyapatite was still the main component of GCE-treated enamel, and the crystallinity was increased, the crystal lattice changed gently with decreased lattice parameter a. These results indicated the potential of GCE in promoting the remineralization of initial enamel carious lesions, and supported the previous hypothesis about GCE mechanism. Combined with the anti-bacteria and demineralization inhibition properties of GCE, the natural G. chinensis may become one more promising agent for caries prevention.
Zhang
Ling-Lin
LL
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
Li
Ji-Yao
JY
Zhou
Xue-Dong
XD
Cui
Fu-Zhai
FZ
Wei
Li
L
D000818:Animals; D002417:Cattle; D018360:Crystallography, X-Ray; D003731:Dental Caries / Q000188:drug therapy*; D003743:Dental Enamel / Q000737:chemistry* / Q000648:ultrastructure*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D029045:Rhus / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission
D004365:Drugs, Chinese Herbal