title: Functional analysis of the inhibitor of apoptosis genes in Antheraea pernyi nucleopolyhedrovirus.
abstract: The inhibitor of apoptosis proteins (IAP) plays an important role in cell apoptosis. We cloned two novel IAP family members, Ap-iap1 and Ap-iap2, from Antheraea pernyi nucleopolyhedrovirus (ApNPV) genome. Ap-IAP1 contains two baculoviral IAP repeat (BIR) domains followed by a RING domain, but Ap-IAP2 has only one BIR domain and RING. The result of transient expression in Spodoptera frugiperda (Sf21) showed that Ap-iap1 blocked cell apoptosis induced by actinomycin D treatment and also rescued the p35 deficient Autographa californica nucleopolyhedrovirus (AcNPV) to replicate in Sf9 cells, while Ap-iap2 does not have this function. Several Ap-IAP1 truncations were constructed to test the activity of BIRs or RING motif to inhibit cell apoptosis. The results indicated that BIRs or RING of Ap-IAP1 had equally function to inhibit cell apoptosis. Therefore deletion of above both of the above domains could not block apoptosis induced by actinomycin D or rescue the replication of AcMNPV Delta p35. We also screened two phage-display peptides that might interact with Ap-IAP1.
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, Wuhan, 430030, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yan
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Deng
authors.forename: Junpeng
authors.initials: J
authors.lastname: Yan
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Wang
authors.forename: Lunguang
authors.initials: L
authors.lastname: Yao
authors.forename: Songya
authors.initials: S
authors.lastname: Lv
authors.forename: Yipeng
authors.initials: Y
authors.lastname: Qi
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity*; D016415:Sequence Alignment; D017384:Sequence Deletion; D018411:Spodoptera / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D051034:Inhibitor of Apoptosis Proteins; D014764:Viral Proteins; D037521:Virulence Factors