title: The transcription factor Mef2 is required for normal circadian behavior in Drosophila.
abstract: The transcription factor Mef2 has well established roles in muscle development in Drosophila and in the differentiation of many cell types in mammals, including neurons. Here, we describe a role for Mef2 in the Drosophila pacemaker neurons that regulate circadian behavioral rhythms. We found that Mef2 is normally produced in all adult clock neurons and that Mef2 overexpression in clock neurons leads to long period and complex rhythms of adult locomotor behavior. Knocking down Mef2 expression via RNAi or expressing a repressor form of Mef2 caused flies to lose circadian behavioral rhythms. These behavioral changes are correlated with altered molecular clocks in pacemaker neurons: Mef2 overexpression causes the oscillations in individual pacemaker neurons to become desynchronized, while Mef2 knockdown strongly dampens molecular rhythms. Thus, a normal level of Mef2 activity is required in clock neurons to maintain robust and accurate circadian behavioral rhythms.
authors.affiliation: Department of Biology, New York University, New York, New York 10003, USA.
authors.forename: Florence J
authors.initials: FJ
authors.lastname: Blanchard
authors.forename: Ben
authors.initials: B
authors.lastname: Collins
authors.forename: Shawn A
authors.initials: SA
authors.lastname: Cyran
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Hancock
authors.forename: Michael V
authors.initials: MV
authors.lastname: Taylor
authors.forename: Justin
authors.initials: J
authors.lastname: Blau
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000502:physiology; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology*; D004330:Drosophila / Q000235:genetics / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D009043:Motor Activity / Q000235:genetics / Q000502:physiology; D018006:Myogenic Regulatory Factors / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000502:physiology*; D010507:Periodicity; D010641:Phenotype; D034622:RNA Interference; D013997:Time Factors
chemical_list: D029721:Drosophila Proteins; C499409:Mef2 protein, Drosophila; D018006:Myogenic Regulatory Factors