Comparison of ganglioside expression between human adipose- and dental pulp-derived stem cell differentiation into osteoblasts.
Human adipose-derived stem cells (hADSCs) and dental pulp-derived stem cells (hDPSCs) have been considered alternative sources of adult stem cells because of their potential to trans-differentiate into multiple cell lineages. This study investigated the possible role of gangliosides in the osteoblast differentiation of hADSCs and hDPSCs. First, we investigated characterization of hADSCs and hDPSCs using FACS analysis. Mesenchymal stem cell specific markers, CD44 and CD105, were expressed but not hematopoietic markers, CD45 and CD117 in both of hADSCs and hDPSCs. High-performance thin-layer chromatography analysis showed that increased gangliosides were associated with differentiation of hADSCs and hDPSCs into osteoblasts. RT-PCR analysis confirmed that osteoblast specific genes, ALP, BMP-2, collagen were expressed in differentiated osteoblasts, however, the another osteoblast specific gene, osteocalcin, was not expressed. When hADSCs and hDPSCs were cultured under osteoblast-differentiation conditions, alkaline phosphatase (ALP) activity was increased in comparison to hADSCs and hDPSCs. Furthermore, specifically both ALP activity and ganglioside expression increased more in hDPSCs-derived osteoblasts than hADSCs-derived osteoblasts. These results suggest that gangliosides play a more important role in regulating the osteoblast-differentiation of hDPSCs compared to hADSCs.
Lee
So Hyun
SH
Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, 570-749, Korea.
Ryu
Jae-Sung
JS
Lee
Jeong-Woong
JW
Kwak
Dong Hoon
DH
Ko
Kisung
K
Choo
Young-Kug
YK
D000273:Adipose Tissue / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003782:Dental Pulp / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D005732:Gangliosides / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism
D005732:Gangliosides; D000469:Alkaline Phosphatase