[Catalase activity in lung, kidney and small bowel non-ischemic in rats after intestinal reperfusion].
OBJECTIVE
This study aimed to assess the catalase activity after ischemia and reperfusion and to study the changes of this antioxidant in organs located far from the initial insult.


METHODS
Eighteen Wistar rats were randomly divided into three groups. 1-Control, 2-Simulation and 3-Ischemia and Reperfusion. In the latter it was done an ischemia of the ileum for 60 minutes followed by reperfusion for 30 minutes. In group 2 only laparotomy was performed. From all animals it was taken segments of the reperfused and non reperfused intestine, as well of the right kidney and lung to be evaluated under light microscopy. Catalase activity was measured in spectrophotometer with a wavelength set to 240 nm. It was used Mann Whitney and Kruskal Wallis statistical tests.


RESULTS
There was a significant increase (p <0.05) in the catalase activity not only at small bowel ischemic and non-ischemic segments but also at lungs. However the enzymatic activity decreases in the kidney. In all organs studied at reperfusion group it was found a slight villi derangement, mild congestion and infiltration with inflammatory cells, and areas of pulmonary atelectasis.


CONCLUSIONS
The intestinal oxidative stress in rats causes biochemical changes at distance, with mobilization of antioxidant defense mechanisms in lung, non-ischemic intestinal segment and kidney, with early decrease in this last organ, however, with no relevant cellular damage.
Ferro
Camila de Oliveira
Cde O
Departamento de Cirurgia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BR. camyoliva@yahoo.com.br
Chagas
Vera Lucia Antunes
VL
de Oliveira
Marcus Fernandes
MF
de Oliveira
Pedro Lagerblad
PL
Schanaider
Alberto
A
D000818:Animals; D002374:Catalase / Q000378:metabolism*; D007421:Intestine, Small / Q000201:enzymology* / Q000473:pathology; D007668:Kidney / Q000201:enzymology* / Q000473:pathology; D008168:Lung / Q000201:enzymology* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000201:enzymology* / Q000473:pathology
D002374:Catalase