title: Isolation and characterization of a virulent bacteriophage AB1 of Acinetobacter baumannii.
abstract: BACKGROUND
  Acinetobacter baumannii is an emerging nosocomial pathogen worldwide with increasing prevalence of multi-drug and pan-drug resistance. A. baumannii exists widely in natural environment, especially in health care settings, and has been shown difficult to be eradicated. Bacteriophages are often considered alternative agent for controlling bacterial infection and contamination. In this study, we described the isolation and characterization of one virulent bacteriophage AB1 capable of specifically infecting A. baumannii.
  
  
  RESULTS
  A virulent bacteriophage AB1, specific for infecting a clinical strain A. baumannii KD311, was first isolated from marine sediment sample. Restriction analysis indicated that phage AB1 was a dsDNA virus with an approximate genome size of 45.2 kb to 46.9 kb. Transmission electron microscopy showed that phage AB1 had an icosahedral head with a non-contractile tail and collar or whisker structures, and might be tentatively classified as a member of the Siphoviridae family. Proteomic pattern of phage AB1, generated by SDS-PAGE using purified phage particles, revealed five major bands and six minor bands with molecular weight ranging from 14 to 80 kilo-dalton. Also determined was the adsorption rate of phage AB1 to the host bacterium, which was significantly enhanced by addition of 10 mM CaCl2. In a single step growth test, phage AB1 was shown having a latent period of 18 minutes and a burst size of 409. Moreover, pH and thermal stability of phage AB1 were also investigated. At the optimal pH 6.0, 73.2% of phages survived after 60 min incubation at 50 degrees C. When phage AB1 was used to infect four additional clinical isolates of A. baumannii, one clinical isolate of Stenotrophomonas maltophilia, and Pseudomonas aeruginosa lab strains PAK and PAO1, none of the tested strains was found susceptible, indicating a relatively narrow host range for phage AB1.
  
  
  CONCLUSIONS
  Phage AB1 was capable of eliciting efficient lysis of A. baumannii, revealing its potential as a non-toxic sanitizer for controlling A. baumannii infection and contamination in both hospital and other public environments.
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, PO Box 08, Tianjin University of Science & Technology, TEDA, Tianjin 300457, PR China. hongjiangyang@tust.edu.cn
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.forename: Shuxiang
authors.initials: S
authors.lastname: Lin
authors.forename: Shiru
authors.initials: S
authors.lastname: Jia
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000302:isolation & purification / Q000821:virology*; D001435:Bacteriophages / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification* / Q000648:ultrastructure; D002122:Calcium Chloride / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D019015:Geologic Sediments / Q000821:virology; D006863:Hydrogen-Ion Concentration; D050296:Microbial Viability; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020543:Proteome / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017906:Siphoviridae / Q000145:classification / Q000648:ultrastructure; D013696:Temperature; D014764:Viral Proteins / Q000032:analysis / Q000737:chemistry; D014771:Virion / Q000737:chemistry / Q000648:ultrastructure; D053585:Virus Attachment
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D020543:Proteome; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014764:Viral Proteins; D004247:DNA; D002122:Calcium Chloride