title: Effect of levofloxacin and pefloxacin on humoral immune response elicited by bovine serum albumin docked in gelatin microparticles and nanoparticles.
abstract: The aim of the present investigation was to study the effect of levofloxacin and pefloxacin on the humoral immune response elicited by bovine serum albumin (BSA) encapsulated in gelatin particulate systems. FITC-BSA (Fluoresceine isothiocynate-bovine serum albumin) was entrapped in gelatin microparticles (GM) and gelatin nanoparticles (GN) prepared by emulsion polymerization and nanoemulsion methods, respectively. The prepared particulate carriers were evaluated for particle size, surface morphology, entrapment efficiency, zeta potential and in vitro antigen release. The optimized formulation of FITC-BSA loaded GM and GN were administered s.c. to albino rats and humoral immune response was measured in terms of systemic IgG antibody titre by ELISA method. The serum IgG response elicited was compared to that was obtained by s.c. administration of either free antigen or antigen emulsified (1:1) with Freund's in complete adjuvant (FIA). The vaccination of 2.41 +/- 1.56 microm sized GM elicited significantly (P<0.05) higher serum IgG response than that obtained with administration of 107 +/- 25 nm sized GN. Similarly, levofloxacin significantly (P<0.05) decreased the antibody titre in rats immunized with BSA docked GM whereas pefloxacin did not reduce the antibody titre significantly. The study will help in programming a new drug management and in characterization of vaccine-drug interaction.
authors.affiliation: Department of Pharmaceutics, Hindu College of Pharmacy, Sonepat, India.
authors.forename: J
authors.initials: J
authors.lastname: Madan
authors.forename: D
authors.initials: D
authors.lastname: Kaushik
authors.forename: S
authors.initials: S
authors.lastname: Sardana
authors.forename: A
authors.initials: A
authors.lastname: Ali
authors.forename: M
authors.initials: M
authors.lastname: Sudhakaran Sudheesh
authors.forename: R
authors.initials: R
authors.lastname: Shankar Pandey
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004339:Drug Compounding; D004355:Drug Stability; D004563:Electrochemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005079:Excipients; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D005780:Gelatin; D005976:Glutaral / Q000737:chemistry; D056724:Immunity, Humoral / Q000187:drug effects*; D007073:Immunoglobulin E / Q000032:analysis / Q000096:biosynthesis; D007155:Immunologic Factors / Q000494:pharmacology; D007202:Indicators and Reagents; D064704:Levofloxacin; D008297:Male; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D015242:Ofloxacin / Q000494:pharmacology*; D010316:Particle Size; D015366:Pefloxacin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000276:immunology*
chemical_list: D000900:Anti-Bacterial Agents; D005079:Excipients; D005456:Fluorescent Dyes; D007155:Immunologic Factors; D007202:Indicators and Reagents; D012710:Serum Albumin, Bovine; D015366:Pefloxacin; D007073:Immunoglobulin E; D064704:Levofloxacin; D005780:Gelatin; D015242:Ofloxacin; D016650:Fluorescein-5-isothiocyanate; D005976:Glutaral