title: Enthalpic studies of xyloglucan-cellulose interactions.
abstract: We report a study of xyloglucan (XG)-cellulose interactions made possible by the preparation of various well-defined cellulosic and xyloglucosidic substrates. Bacterial microcrystalline cellulose (BMCC) as well as cellulose whiskers (CellWhisk) were used as cellulosic substrates. Xyloglucosidic substrates were obtained from Rubus cells and Tamarindus indica seeds. Different primary structure characteristics of XGs such as the backbone length and the nature of the side chains, as well as their repartition, were considered in order to examine the influence of the primary structure on their interaction capacity. Two complementary approaches were carried out: first, the determination of adsorption isotherms and its associated models, and second, an enthalpic study using isothermal titration calorimetry (ITC). This study highlighted that an increase of XG interaction capacity occurred with increasing XG molecular weight. Furthermore, we determined that a minimum of 12 glucosyl residues on the backbone is required to observe significant interactions. Moreover, both the presence of trisaccharidic side chains with fucosyl residues and an increase of unsubstituted glucosyl residues enhanced XG-cellulose interactions. The evolution of adsorption isotherms with temperature and ITC measurements showed that two different processes were occurring, one exothermic and one endothermic, respectively. Although the presence of an exothermic interaction mechanism has long been established, the presence of an endothermic interaction mechanism has never been reported.
authors.affiliation: INRA, UR1268 Biopolymères Interactions Assemblages, BP 71627, F-44316 Nantes, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Lopez
authors.forename: Hervé
authors.initials: H
authors.lastname: Bizot
authors.forename: Gérard
authors.initials: G
authors.lastname: Chambat
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Marais
authors.forename: Agata
authors.initials: A
authors.lastname: Zykwinska
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Ralet
authors.forename: Hugues
authors.initials: H
authors.lastname: Driguez
authors.forename: Alain
authors.initials: A
authors.lastname: Buléon
mesh_terms: D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry* / Q000302:isolation & purification; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification; D041822:Gluconacetobacter xylinus / Q000737:chemistry; D008958:Models, Molecular; D027824:Rosaceae / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D029861:Tamarindus / Q000737:chemistry; D013816:Thermodynamics; D014990:Xylans / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005936:Glucans; D014990:Xylans; C029353:xyloglucan; D002482:Cellulose; C109691:microcrystalline cellulose