title: Synthesis of magnetic and fluorescent bifunctional nanocomposites and their applications in detection of lung cancer cells in humans.
abstract: We developed a novel strategy to detect lung cancer cells by utilizing magnetic and fluorescent bifunctional nanocomposites (BNPs) in combination with monoclonal anti-carcinoembryonic antigen (CEA) antibodies. The BNPs, consisting of silica-coated superparamagnetic nanoparticles and quantum dots (QDs), exhibited high luminescence and were easily separated in an external magnetic field. The binding specificity of the antibody-conjugated BNPs (immunonanoparticles) were confirmed via incubating with human lung adenocarcinoma SPCA-1 cells, human leukemic K562 cells and human embryonic lung fibroblasts MRC-5 cells. Further experiments demonstrated that the as-prepared immunonanoparticles can efficiently capture and detect cancer cells in pleural effusion from lung cancer patients. These results suggest that this method, of which the detection procedures are completed within 1h, could be applied to the rapid and cost-effective monitoring of cancer cells in clinical samples.
authors.affiliation: Department of Chemistry, Life and Environment Science College, Shanghai Normal University, Shanghai, PR China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Ma
authors.forename: Qishi
authors.initials: Q
authors.lastname: Fan
authors.forename: Lianhui
authors.initials: L
authors.lastname: Wang
authors.forename: Nengqin
authors.initials: N
authors.lastname: Jia
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Gu
authors.forename: Hebai
authors.initials: H
authors.lastname: Shen
mesh_terms: D000328:Adult; D002272:Carcinoembryonic Antigen / Q000737:chemistry; D045744:Cell Line, Tumor; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D020014:K562 Cells; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D008280:Magnetics; D008297:Male; D008875:Middle Aged; D053761:Nanocomposites / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D045663:Quantum Dots; D014376:Tuberculosis / Q000378:metabolism
chemical_list: D002272:Carcinoembryonic Antigen; D005456:Fluorescent Dyes