Distinct circulating recombinant HIV-1 strains among injecting drug users and sex workers in Afghanistan.
Little information is available regarding a circulating HIV genotype among high-risk groups in Afghanistan; we describe HIV genotypes among injecting drug users (IDUs) and sex workers (SWs) in four Afghan cities. Participants completed behavioral questionnaires and HIV testing. Western blot-confirmed specimens had peripheral mononuclear blood cells isolated for genotyping. Analysis of recombinants was done by bootscanning and manual sequence alignment. The single SW sample harbored a CRF01_AE strain. Of 10 IDUs available for analysis, all were CRF35_AD and from Hirat. Analyzed subregions (gag p17 and env C1-C5) revealed close homology between the Hirat specimens. Three distinct subclusters comprising two or three strains were identified, whereas two other strains were generally equidistant from previously identified Kabul strains. Results suggest that the nascent HIV epidemic among IDUs in Hirat is largely, if not entirely, subtype CRF35_AD, and the close homology suggests recent infection; harm reduction should be supported to avert further transmission.
Sanders-Buell
Eric
E
United States Military HIV Research Program, Rockville, Maryland, USA.
Bose
Meera
M
Nasir
Abdul
A
Todd
Catherine S
CS
Stanekzai
M Raza
MR
Tovanabutra
Sodsai
S
Scott
Paul T
PT
Strathdee
Steffanie A
SA
Tjaden
Jeffrey
J
Michael
Nelson L
NL
McCutchan
Francine E
FE
D000328:Adult; D000346:Afghanistan / Q000453:epidemiology; D004196:Disease Outbreaks; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D011477:Sex Work; D015819:Substance Abuse, Intravenous / Q000150:complications*