title: Structural stability and surface activity of sunflower 2S albumins and nonspecific lipid transfer protein.
abstract: The structural and interfacial properties of five different fractions of sunflower ( Helianthus annuus L.) seed storage proteins were studied. The fractions comprised lipid transfer protein (LTP), the methionine-rich 2S albumin SFA8 (sunflower albumin 8), and three mixtures of non-methionine-rich 2S albumins called Alb1 and Alb2 proteins (sunflower albumins 1 and 2). Heating affected all of the proteins studied, with SFA8 and LTP becoming more surface active than the native proteins after heating and cooling. LTP appeared to be less thermostable than homologous LTPs from other plant species. SFA8 generated the greatest elastic modulus and formed the most stable emulsions, whereas LTP showed poorer emulsification properties. The mixed 2S albumin fractions showed moderate levels of surface activity but had the poorest emulsification properties among the proteins studied.
authors.affiliation: Department of Plant Physiology and Molecular Plant Biology, Eötvös Loránd University, Pázmány P stny 1/C, H-1117 Budapest, Hungary. bberecz@caesar.elte.hu
authors.forename: Bernadett
authors.initials: B
authors.lastname: Berecz
authors.forename: E N Clare
authors.initials: EN
authors.lastname: Mills
authors.forename: László
authors.initials: L
authors.lastname: Tamás
authors.forename: Ferenc
authors.initials: F
authors.lastname: Láng
authors.forename: Peter R
authors.initials: PR
authors.lastname: Shewry
authors.forename: Alan R
authors.initials: AR
authors.lastname: Mackie
mesh_terms: D055516:2S Albumins, Plant / Q000737:chemistry* / Q000302:isolation & purification; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002942:Circular Dichroism; D004355:Drug Stability; D004548:Elasticity; D004655:Emulsions / Q000737:chemistry; D006368:Helianthus / Q000737:chemistry*; D006358:Hot Temperature; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D017433:Protein Structure, Secondary; D012639:Seeds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D055516:2S Albumins, Plant; D002352:Carrier Proteins; D004655:Emulsions; D010940:Plant Proteins; C030363:lipid transfer protein