Identification of a functional medaka heat shock promoter and characterization of its ability to induce exogenous gene expression in medaka in vitro and in vivo.
Heat shock protein promoters (hsp promoters) are powerful tools for investigating gene functions, as the expression of targeted genes can be controlled simply by heating. However, there have been no reports of the utilization of an endogeneous medaka (Oryzias latipes) hsp promoter to induce exogenous gene expression in medaka. We identified and cloned a functional medaka hsp promoter (olphsp70.1) and verified its ability to act as an inducible promoter both in vitro and in vivo. The hsp promoter efficiently induced exogenous gene expression in cultured cells, developing embryos, and also in adult fishes. When used to control the expression of Venus, a variant of yellow fluorescent protein, in transgenic medaka, the hsp promoter was functional in all tissues except for the gonads of adults. These results indicate that the medaka hsp promoter can be a powerful tool for inducing exogenous gene expression and investigating gene functions both in vitro and in vivo in medaka.
Oda
Shoji
S
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwa-no-ha, Kashiwa, Chiba 277-8562, Japan. odasho@k.u-tokyo.ac.jp
Mikami
Sachi
S
Urushihara
Yusuke
Y
Murata
Yasuhiko
Y
Kamei
Yasuhiro
Y
Deguchi
Tomonori
T
Kitano
Takeshi
T
Fujimori
Kazuhiro E
KE
Yuba
Shunsuke
S
Todo
Takeshi
T
Mitani
Hiroshi
H
D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian; D005786:Gene Expression Regulation / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D009990:Oryzias / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic
D006360:Heat-Shock Proteins; D008156:Luciferases