[Mechanisms of decorin inhibiting epithelial-to-mesenchymal transition induced by transforming growth factor beta1 in renal tubular epithelial cells].
OBJECTIVE
To investigate the mechanisms of decorin inhibiting epithelial-to-mesenchymal transition (EMT) induced by transforming growth factor beta1 (TGF-beta1) in renal tubular epithelial cells.


METHODS
HK-2 cells in vitro were divided into 4 groups: (1) negative control group; (2) decorin group, added with decorin 100 ng/ml ; (3) TGF-beta1 group, added with TGF-beta1 10 ng/ml; (4) decorin and TGF-beta1 group, added with decorin 100 ng/ml and TGF-beta1 10 ng/ml. The protein level of phosphor-ERK, phosphor-PI3K, phosphor-Smad(3) and beta-catenin was detected by Western blotting method. The snail mRNA level was tested by real time-PCR, while the lymphoid enhancer factor-1 (LEF-1) mRNA level was measured by RT-PCR.


RESULTS
The snail (2.59 +/- 0.70:1.02 +/- 0.13) and LEF-1 mRNA (1.85 +/- 0.08:0.30 +/- 0.11) were significantly up-regulated, meanwhile the protein level of phosphor-ERK (1.11 +/- 0.09:0.47 +/- 0.07), phosphor-PI3K (14.79 +/- 1.02:2.48 +/- 0.06), phosphor-Smad(3) (0.95 +/- 0.02:0.08 +/- 0.01) and beta-catenin (1.46 +/- 0.20:0.49 +/- 0.05) were significantly increased in TGF-beta1 group compared to control group, while there were no statistically significant difference in all figures between control group and decorin group. The phosphor-ERK protein level (0.58 +/- 0.08) and the snail mRNA level (1.24 +/- 0.03) were significantly down-regulated in TGF-beta1 and decorin group compared to TGF-beta1 group, however there were no statistically significant differences in the level of phosphor-PI3K (15.84 +/- 1.64), phosphor-Smad(3) (0.90 +/- 0.04) and beta-catenin (1.42 +/- 0.09) between these two groups.


CONCLUSIONS
Decorin inhibited EMT induced by TGF-beta1 which may be through blocking the ERK signal transduction pathway.
Wang
Jin-ya
JY
Department of Pediatrics, Second Hospital Affiliated to Nanjing Medical University, Nanjing 210003, China.
Bao
Hua-ying
HY
Huang
Song-ming
SM
Zhang
Ai-hua
AH
Pan
Xiao-qin
XQ
Fei
Li
L
Chen
Rong-hua
RH
D054337:Cell Dedifferentiation / Q000187:drug effects*; D002478:Cells, Cultured; D058575:Decorin / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology; D005353:Fibronectins; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology / Q000473:pathology*; D011509:Proteoglycans; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D058575:Decorin; D005353:Fibronectins; D011509:Proteoglycans; D053773:Transforming Growth Factor beta1