[A comparative study on HPLC fingerprint of Semen Strychni and the processed].
OBJECTIVE
To establish the fingerprint of Semen Strychni and the processed by HPLC.


METHODS
The HPLC Method was used, the chromatgraphic conditions were as follows: Chromatographic column: Lichrospher C18 (250 mm x 4.6 mm, 5 microm), mobile phase: solvent A consisted of water-ethanoic acid-triethylamine (100: 0.2: 0.2), solvent B contained acetonitril (gradient elution), column temperature: 30 degrees C, flow rate: 1.0 mL/min, detection wavelength: 254 nm.


RESULTS
The similar degree of 10 batch samples buiding sharing mode of crude drug and processed product was above 0.9. 18 common peaks in chromatograms were separated from 10 batches of Semen Strychni samples and 21 common peaks in chromatograms were separated from 10 batches of the processed.


CONCLUSIONS
This study establishes the HPLC fingerprint commonmode, and researches the difference between Semen Strychni and the processed in HPLC fingerprint.
Li
Jun-song
JS
Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of TCM, Nanjing 210029, China. lijunsong1964@163.com
Wang
Dan-dan
DD
Cai
Bao-chang
BC
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D010598:Pharmacognosy; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D013331:Strychnine / Q000031:analogs & derivatives / Q000032:analysis; D031451:Strychnos / Q000737:chemistry* / Q000254:growth & development
D004365:Drugs, Chinese Herbal; C083806:brucine; D013331:Strychnine