Effects of low-intensity microwave radiation on Tribolium castaneum physiological and biochemical characteristics and survival.
The red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae) is a widespread pest that lives in, and feeds on, wheat flour. Here, we studied the effects of low-intensity microwave radiation (LIMR; <or=2.0kW/kg) on physiological and biochemical characteristics of T. castaneum, and compared them to the effects of heat conduction treatment, to provide a theoretical basis for using LIMR for pest control. Lethal model equations with respect to temperature were shown to provide acceptable fitting accuracy for the effects of LIMR treatment. Semi-lethal and lethal temperatures induced through LIMR (48 degrees C and 50 degrees C, respectively) for T. castaneum were lower than those induced through heat conduction (50 degrees C and 52 degrees C). When T. castaneum were subjected to LIMR, the insects' moisture content, pH values, alkaline phosphatase and acetyl cholinesterase activity were all lower than when the insects were subjected to heat conduction. Peroxide values and total free amino acid content were higher, and protein subunits molecular weights were lower when T. castaneum were exposed to LIMR than to heat; moreover, after LIMR exposure, the amino acid composition of T. castaneum was changed and the insect's DNA was damaged.
Lu
Huanghua
H
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province, PR China.
Zhou
Jicheng
J
Xiong
Shanbai
S
Zhao
Siming
S
D000110:Acetylcholinesterase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001834:Body Water; D004249:DNA Damage; D006863:Hydrogen-Ion Concentration; D008872:Microwaves / Q000009:adverse effects*; D009026:Mortality; D010545:Peroxides / Q000378:metabolism; D010571:Pest Control / Q000379:methods*; D016019:Survival Analysis; D013696:Temperature; D014231:Tribolium / Q000502:physiology* / Q000528:radiation effects*
D000596:Amino Acids; D010545:Peroxides; D000110:Acetylcholinesterase; D000469:Alkaline Phosphatase