title: Simultaneous measurement of pazufloxacin, ciprofloxacin, and levofloxacin in human serum by high-performance liquid chromatography with fluorescence detection.
abstract: In this study, three fluoroquinolones, pazufloxacin, ciprofloxacin and levofloxacin, were simultaneously determined in spiked human serum by high-performance liquid chromatography (HPLC) method with fluorescence detection. Chromatography was performed using a C8 column with an isocratic mobile phase consisting of 1% triethylamine (pH 3.0)/acetonitrile (86/14, v/v). Protein precipitation was conducted using perchloric acid and methanol. The calibration curves for the three fluoroquinolones were linear over concentrations ranging from 0.1 to 20.0 microg/mL. The within-day and between-day coefficients of variation obtained from three fluoroquinolones were less than 7%, and relative errors ranged from -1.6% to 9.3%. Mean recoveries of pazufloxacin, ciprofloxacin, and levofloxacin from spiked human serum were 97%, 88%, and 90%, respectively. The proposed method proved to be simple and reliable for the determination of three fluoroquinolones.
authors.affiliation: Pharmacokinetic Laboratory, Department of Clinical Pharmacy, Center for Clinical Pharmacy and Sciences, School of Pharmacy, Kitasato University, 5-9-1 Shirogane, Minato, Tokyo 108-8641, Japan. watabesa@gmail.com
authors.forename: Satoshi
authors.initials: S
authors.lastname: Watabe
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yokoyama
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Nakazawa
authors.forename: Kimikazu
authors.initials: K
authors.lastname: Shinozaki
authors.forename: Rika
authors.initials: R
authors.lastname: Hiraoka
authors.forename: Kei
authors.initials: K
authors.lastname: Takeshita
authors.forename: Yukio
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000097:blood* / Q000737:chemistry; D004355:Drug Stability; D024841:Fluoroquinolones / Q000097:blood* / Q000737:chemistry; D006801:Humans; D016018:Least-Squares Analysis; D064704:Levofloxacin; D015242:Ofloxacin / Q000097:blood* / Q000737:chemistry; D010078:Oxazines / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D024841:Fluoroquinolones; D010078:Oxazines; C078052:pazufloxacin; D002939:Ciprofloxacin; D064704:Levofloxacin; D015242:Ofloxacin