title: Synergistic induction of profibrotic PAI-1 by TGF-β and radiation depends on p53.
abstract: Radiation-induced fibrosis is a severe side effect of radiotherapy. TGF-β and radiation synergistically induce expression of the profibrotic PAI-1 gene and this cooperation potentially involves p53. Here, we demonstrate that p53 is both indispensable and sufficient for the radiation effect inducing synergistic activation of PAI-1 by radiation and TGF-β.
authors.affiliation: Department of Cell Biology, University Medical Center Groningen, University of Groningen, The Netherlands.
authors.forename: Maarten
authors.initials: M
authors.lastname: Niemantsverdriet
authors.forename: Edwin
authors.initials: E
authors.lastname: de Jong
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Langendijk
authors.forename: Harm H
authors.initials: HH
authors.lastname: Kampinga
authors.forename: Robert P
authors.initials: RP
authors.lastname: Coppes
mesh_terms: D045744:Cell Line, Tumor; D004307:Dose-Response Relationship, Radiation; D005355:Fibrosis / Q000209:etiology / Q000235:genetics; D015870:Gene Expression / Q000235:genetics / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D009369:Neoplasms / Q000235:genetics* / Q000532:radiotherapy*; D010766:Phosphorylation; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D011836:Radiation Tolerance / Q000235:genetics*; D011878:Radiotherapy / Q000009:adverse effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation / Q000235:genetics / Q000528:radiation effects; D053773:Transforming Growth Factor beta1 / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D017395:Plasminogen Activator Inhibitor 1; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D016159:Tumor Suppressor Protein p53