Influence of the lung mechanical ventilation with injurious parameters on 7-ketocholesterol synthesis in Sus Scrofa.
The aim of work was to investigate changes of 7-ketocholesterol synthesis in alveolar macrophages in the dynamic of lung mechanical ventilation with injurious parameters. The goal of in vitro part of work was to observe influence of 7-ketocholesterol on iNOS and MIP1 Beta production in bronchoalveolar lavage fluid (BALF) cells. We used 17 healthy domestic pigs randomly assigned into two treatment groups: group I with mechanical ventilation with physiological parameters; group || underwent injurious ventilation with high volume tidal (VT) and low positive end expiratory pressure (PEEP). ells were analyzed for CYP27A1 protein and gene expression levels, 7-ketocholesterol production. In alveolar macrophages of group ||, we obtained increase of production of CYP27A1 protein and 7-ketocholesterol, as well as the expression of the CYP27A1 gene at the 2nd hour of ventilation. In the in vitro experiments we show dose-dependent increase of MIP1 Beta and decrease of CYP27A1, iNOS protein production after 7-ketocholesterol treatment.
Klimenko
Oxana V
OV
Department of Pediatrics and Adolescent Medicine, 1st School of Medicine, Charles University, Prague, Czech Republic. O_klimenko@hotmail.com
Vobruba
Vaclav
V
Martasek
Pavel
P
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D053493:Cholestanetriol 26-Monooxygenase / Q000235:genetics / Q000378:metabolism; D007653:Ketocholesterols / Q000096:biosynthesis / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000201:enzymology; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D012121:Respiration, Artificial; D034421:Sus scrofa / Q000378:metabolism; D013997:Time Factors
D048868:Adaptor Proteins, Signal Transducing; D007653:Ketocholesterols; D052247:Nitric Oxide Synthase Type II; D053493:Cholestanetriol 26-Monooxygenase; C003001:7-ketocholesterol