title: A new mediator method for BOD measurement under non-deaerated condition.
abstract: Monitoring biochemical oxygen demand (BOD) by mediator method (BOD(Med)) has been developed for recent years and deaerated condition was generally adopted to avoid the effect of oxygen, but the deaerated condition was unfavorable in practical applications. Herein, we first proposed another way to explore non-deaerated BOD(Med) (called NDA-BOD(Med)) method utilizing ferricyanide, which was reduced by Escherichia coli upon catalyzing organic substrate to produce ferrocyanide. We attempted to explain the feasibility of NDA-BOD(Med) by the two aspects. Firstly, the obtained biodegradation efficiencies of the bacteria under the deaerated and non-deaerated conditions were similar, and the concentration of O(2) (0.25mM at 8mg/L O(2)) is 1-2 order of magnitude lower than that of mediator commonly used (55mM ferricyanide), so the effect of O(2) to measurements could be neglected. Secondly, the relationship between the artificial and the natural electron acceptor was investigated, and it was found that the oxygen consumption in the NDA-BOD(Med) measurement was mainly contributed to endogenous values. Furthermore, the performance of present NDA-BOD(Med) was reported, and this method was optimized for measuring the low-concentration samples, synthetic wastewater and real polluted wastewater. The NDA-BOD(Med) provides a simple and efficient way in rapid BOD determinations, especially advantageous for in situ monitoring of water system.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Shang
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Changyu
authors.initials: C
authors.lastname: Liu
authors.forename: Bailin
authors.initials: B
authors.lastname: Zhang
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dong
mesh_terms: D001673:Biodegradation, Environmental; D002384:Catalysis; D004563:Electrochemistry / Q000379:methods; D004583:Electrons; D004926:Escherichia coli / Q000378:metabolism; D005292:Ferricyanides / Q000737:chemistry; D005295:Ferrocyanides / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry*; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000032:analysis; D018508:Water Purification / Q000379:methods
chemical_list: D005292:Ferricyanides; D005295:Ferrocyanides; D009930:Organic Chemicals; D014874:Water Pollutants, Chemical; C007931:hexacyanoferrate III; D018698:Glutamic Acid; C020354:hexacyanoferrate II; D005947:Glucose; D010100:Oxygen