title: Prevalence and antimicrobial resistance of Salmonella serotypes in chickens from retail markets in Yaounde (Cameroon).
abstract: From February 2006 to January 2007, 150 chickens were collected from eight retail markets in Yaounde, and 90 (60%) tested positive for Salmonella. Seventy-nine chickens were contaminated with only one Salmonella serotype, 10 with two different serotypes, and 1 with four serotypes. The most prevalent serotypes were Enteritidis (47 strains) and Hadar (29 strains). The isolates were tested for their susceptibilities to amoxicillin, amoxicillin/clavulanic acid, cefoxitin, cefotaxime, gentamicin, streptomycin, tetracycline, chloramphenicol, sulfonamides, nalidixic acid, ciprofloxacin, and trimethoprim/sulfamethazole by disk diffusion assay. Minimum inhibitory concentrations of ampicillin, streptomycin, tetracycline, sulfonamides, and nalidixic acid were determined for the resistant strains by agar dilution method. Eleven isolates (10.7%) of the 103 tested were susceptible to all antimicrobials. Resistance was most observed to tetracycline (84.5%), streptomycin (44.7%), and nalidixic acid (34%). Forty-one isolates (39.8%) were multidrug resistant (resistant to three or more antimicrobials from different classes), of which 68.3% were Hadar and 21.9% Enteritidis. The most frequent resistant pattern in Hadar was streptomycin-tetracycline-nalidixic acid. These results highlight once more the need for surveillance of Salmonella contamination in poultry.
authors.affiliation: Laboratory of Bacteriology, Pasteur Centre of Cameroon, Yaounde, Cameroon.
authors.forename: Marguerite
authors.initials: M
authors.lastname: Wouafo
authors.forename: Ariane
authors.initials: A
authors.lastname: Nzouankeu
authors.forename: Junie Atangana
authors.initials: JA
authors.lastname: Kinfack
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Fonkoua
authors.forename: Guy
authors.initials: G
authors.lastname: Ejenguele
authors.forename: Thomas
authors.initials: T
authors.lastname: Njine
authors.forename: Antoinette
authors.initials: A
authors.lastname: Ngandjio
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002163:Cameroon; D002645:Chickens / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005506:Food Contamination; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D012475:Salmonella / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D012703:Serotyping
chemical_list: D000900:Anti-Bacterial Agents