Immunosuppressive activity of ethanolic extract of seeds of Moringa oleifera Lam. in experimental immune inflammation.
BACKGROUND
Traditionally, the plant Moringa oleifera Lam. (Moringaceae) is used for the treatment of ascites and rheumatism, while the dried seeds of the plant are used as an 'anti-allergic' agent.


OBJECTIVE
The aim of the study was to assess the efficacy of ethanolic extract of seeds from Moringa oleifera Lam. in experimental immune inflammation.


METHODS
Circulatory and splenic leukocyte counts, delayed-type hypersensitivity reactions and humoral antibody responses were measured in mice using SRBC as the antigen. In addition, macrophage phagocytosis was measured by the carbon clearance test.


RESULTS
The extract dose-dependently (50, 100 and 200mg/kg) inhibited spleen weight as well as circulatory leukocyte and splenocyte counts. The delayed-type hypersensitivity reaction was significantly inhibited (P<0.01) by decreasing the mean foot pad thickness at 48 h. The production of the humoral antibody titer was significantly ameliorated at a dose of 100 and 200mg/kg (P<0.05 and P<0.01, respectively). Furthermore, the extract caused a down-regulation of macrophage phagocytosis due to carbon particles.


CONCLUSIONS
Taken together, the above findings suggest that the seeds of Moringa oleifera have immunosuppressive activity.
Mahajan
Shailaja G
SG
Department of Pharmacology, L.M. College of Pharmacy, Ahmedabad 380009, India.
Mehta
Anita A
AA
D000818:Animals; D000917:Antibody Formation; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry*; D006968:Hypersensitivity, Delayed; D007166:Immunosuppressive Agents / Q000494:pharmacology* / Q000627:therapeutic use; D007249:Inflammation / Q000188:drug therapy* / Q000276:immunology; D007958:Leukocyte Count; D051379:Mice; D029588:Moringa oleifera / Q000196:embryology*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D012639:Seeds / Q000737:chemistry*
D007166:Immunosuppressive Agents; D010936:Plant Extracts; D000431:Ethanol