title: MVH in piRNA processing and gene silencing of retrotransposons.
abstract: VASA is an evolutionarily conserved RNA helicase essential for germ cell development. The mouse PIWI family proteins MILI and MIWI2 are involved in production of Piwi-interacting RNAs (piRNAs) in fetal male germ cells through a ping-pong amplification cycle. Expression of retrotransposons is elevated in MILI- and MIWI2-deficient male germ cells due to defective de novo DNA methylation, which is presumably caused by impaired piRNA expression. Here, we report that essentially the same abnormalities are observed in MVH (mouse VASA homolog)-deficient mice. Comprehensive analysis of piRNAs in MVH-deficient fetal male germ cells showed that MVH plays crucial roles in the early phase of the ping-pong amplification cycle.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Osaka 565-0871, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Kuramochi-Miyagawa
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Watanabe
authors.forename: Kengo
authors.initials: K
authors.lastname: Gotoh
authors.forename: Kana
authors.initials: K
authors.lastname: Takamatsu
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Chuma
authors.forename: Kanako
authors.initials: K
authors.lastname: Kojima-Kita
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Shiromoto
authors.forename: Noriko
authors.initials: N
authors.lastname: Asada
authors.forename: Atsushi
authors.initials: A
authors.lastname: Toyoda
authors.forename: Asao
authors.initials: A
authors.lastname: Fujiyama
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Totoki
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Shibata
authors.forename: Tohru
authors.initials: T
authors.lastname: Kimura
authors.forename: Norio
authors.initials: N
authors.lastname: Nakatsuji
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Noce
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sasaki
authors.forename: Toru
authors.initials: T
authors.lastname: Nakano
mesh_terms: D000818:Animals; D060565:Argonaute Proteins; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism*; D019175:DNA Methylation; D005786:Gene Expression Regulation; D020868:Gene Silencing; D007426:Genes, Intracisternal A-Particle / Q000235:genetics*; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology; D013737:Testis / Q000378:metabolism
chemical_list: D060565:Argonaute Proteins; C448522:Piwil1 protein, mouse; C448521:Piwil2 protein, mouse; D011506:Proteins; D034741:RNA, Small Interfering; C090864:Ddx4 protein, mouse; D053487:DEAD-box RNA Helicases