Fiber-modified adenovirus can mediate human adipose tissue-derived mesenchymal stem cell-based anti-angiogenic gene therapy.
A fiber-modified adenovirus (rAd5F11B), loaded with the Kringle1-5 gene (rAd-K1-5) was used to infect human adipose tissue-derived mesenchymal stem cells (HAMSCs). At a multiplicity of infection of 20, the transfection efficiency in HAMSCs was 90% and the cell expansion and differentiation of infected HAMSCs were not significantly suppressed. HAMSCs infected with rAd-K1-5 expressed the exogenous Kringle1-5 protein, an angiogenic inhibitor, and conditioned media from HAMSCs expressing the Kringle1-5 protein blocked VEGF-induced neovascularization both in vitro and in vivo. rAd5F11B may therefore be a promising gene transfer vector in HAMSCs-based anti-angiogenic gene therapy because of its low toxicity and high transfection efficiency.
Liu
Haixia
H
Department of Gynecological Oncology, Affiliated Tumor Hospital of Harbin Medical University, 150081 Harbin, China.
Chu
Yinzhu
Y
Lou
Ge
G
D000256:Adenoviridae / Q000235:genetics; D000273:Adipose Tissue / Q000166:cytology; D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D002470:Cell Survival; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude
D020533:Angiogenesis Inhibitors; D036022:Capsid Proteins