Production of neo-fructooligosaccharides using free-whole-cell biotransformation by Xanthophyllomyces dendrorhous.
The effects of production parameters on the biotransformation of sucrose were investigated to enhance the yield of neo-FOS by Xanthophyllomyce dendrorhous cells. Cells showed optimal beta-fructofuranosidase activity at neutral pH condition and the yield of neo-FOS showed no significant differences between buffer and buffer-free systems. Cell concentration negatively affected the maximum neo-FOS yield. Sucrose concentration positively increased the maximum yield of neo-FOS. Elevating the reaction temperature to 30 degrees C, the neo-FOS productivity increased 1.85-fold compared with that at 20 degrees C. Meanwhile, cell age of 32 h enabled the biotransformation of sucrose more efficiently. In addition, free cells exhibited a higher productivity over immobilized cells. The maximum neo-FOS concentration finally reached 227.72 g/l from 400 g/l sucrose under the optimal conditions.
Ning
Yawei
Y
State Key Laboratory of Food Science and Technology, Wuxi, Jiangsu, China.
Wang
Jinpeng
J
Chen
Jing
J
Yang
Na
N
Jin
Zhengyu
Z
Xu
Xueming
X
D001487:Basidiomycota / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D001711:Biotransformation / Q000187:drug effects; D002021:Buffers; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D009844:Oligosaccharides / Q000096:biosynthesis*; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D043324:beta-Fructofuranosidase / Q000378:metabolism
D002021:Buffers; D009844:Oligosaccharides; C116580:fructooligosaccharide; D013395:Sucrose; D043324:beta-Fructofuranosidase