title: Biochemical and enzymatic properties of a fibrinolytic enzyme from Pleurotus eryngii cultivated under solid-state conditions using corn cob.
abstract: Biochemical and enzymatic properties of a fibrinolytic enzyme purified from Pleurotus eryngii cultivated under solid-state conditions using corn cob as energy source were investigated. The molecular mass of the enzyme was estimated to be 14 kDa by SDS-PAGE. The enzyme exhibited the highest activity (28.96 mol/min/mg) for the substrate tosyl-Gly-Pro-Lys-p-nitroanilide. K(m) and V(max) values were 0.18 mM and 53.5 U/ml, respectively. The enzyme was completely inhibited by 1.0 mM phenylmethylsulfonyl fluoride (PMSF). The N-terminal sequence was A-M-D-S-Q-T-D-A-S-Y-G-LA-N-D. This sequence exhibited a high degree of similarity to the N-terminal sequences of the subtilisin-like serine proteases. The enzyme was very stable at pH 4.0-6.0 with an optimum pH 5.0 at 40 degrees C. The enzyme rapidly hydrolyzed the A alpha-chain of fibrinogen within 5 min of incubation, followed by the B beta-chain after 10 min. The fibrinolytic enzyme from P. eryngii cultivated under solid-state conditions using corn cob could be potentially exploited in thrombolytic therapy.
authors.affiliation: Department of Biochemical and Chemical Engineering, Chosun University, Gwangju, Republic of Korea.
authors.forename: Wol-Suk
authors.initials: WS
authors.lastname: Cha
authors.forename: Sang-Shin
authors.initials: SS
authors.lastname: Park
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: DuBok
authors.initials: D
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D005342:Fibrinolysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D020076:Pleurotus / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D003313:Zea mays
chemical_list: D004791:Enzyme Inhibitors; D004798:Enzymes; D010664:Phenylmethylsulfonyl Fluoride