title: [Impact of rhG-CSF on Sphingosine 1-phosphate receptor 1 expression in CD4+ T cells of donor peripheral blood].
abstract: CD4+ T cells mainly interact with Sphingosine 1-phosphate (S1P) to regulate immune function through Sphingosine 1-phosphate receptor 1 (S1P1). This study was aimed to investigate the effects of recombinant human granulocyte-colony-stimulating factor (rhG-CSF) mobilization on S1P1 expression in CD4+ T cells of donor's peripheral blood. The CD4+T cells of peripheral blood were isolated by magnetic beads from 17 allo-hematopoietic stem cell transplantation (allo-HSCT) donors before and at fourth day of mobilization with rhG-CSF. The S1P1 expression was detected by real time quantitative PCR in the RNA extracted from CD4+ T cells collected before and after rhG-CSF mobilization. The results showed that the expression of S1P1 was found in CD4+ cells before and after rhG-CSF mobilization, but the expression level of SIP1 in CD4+ cells after rhG-CSF mobilization was significantly lower than that before rhG-CSF mobilization (p<0.01). It is concluded that the mobilization with rhG-CSF obviously down-regulates the expression of S1P1 in CD4+ T cells of donor's peripheral blood.
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhu
authors.forename: Wan-Ming
authors.initials: WM
authors.lastname: DA
authors.forename: Chun-Ji
authors.initials: CJ
authors.lastname: Gao
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Yü
authors.forename: Wen-Rong
authors.initials: WR
authors.lastname: Huang
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D049349:Receptors, Lysosphingolipid / Q000378:metabolism*; D011994:Recombinant Proteins; D000081025:Sphingosine-1-Phosphate Receptors
chemical_list: D049349:Receptors, Lysosphingolipid; D011994:Recombinant Proteins; C477362:S1PR1 protein, human; D000081025:Sphingosine-1-Phosphate Receptors; D016179:Granulocyte Colony-Stimulating Factor