Ultrasensitive and selective DNA detection based on nicking endonuclease assisted signal amplification and its application in cancer cell detection.
By significantly circumventing the typical problem of high background induced by excess hemin itself, an ultrasensitive DNA amplified detection method is developed based on nicking endonuclease (NEase) assisted strand scission cycle, which is further successfully applied in cancer cell detection.
Bi
Sai
S
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Zhang
Jilei
J
Zhang
Shusheng
S
D045744:Cell Line, Tumor; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism*; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis*; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity
D004247:DNA; D004720:Endonucleases