title: Visualization of G-quadruplexes by using a BODIPY-labeled macrocyclic heptaoxazole.
abstract: A BODIPY-labeled macrocyclic heptaoxazole, L1BOD-7OTD, was developed as a fluorescent ligand for G-quadruplexes. The results of the study show that L1BOD-7OTD both selectively induces the formation of intramolecular G-quadruplexes from some G-quadruplex forming oligonucleotides (GFOs). In addition, the labelled macrocyclic heptaozaxole strongly binds to and stabilizes intramolecular G-quadruplexes. Moreover, this substance can be used to directly visualize the G-quadruplexes in the form of green fluorescence. Finally, the possibility that G-quadruplexes form in the cells was demonstrated by using of L1BOD-7OTD.
authors.affiliation: Department of Biotechnology and Life Science Faculty of Technology, Tokyo University of Agriculture and Technology (TUAT), Koganei, Tokyo 184-8588, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tera
authors.forename: Keisuke
authors.initials: K
authors.lastname: Iida
authors.forename: Kazunori
authors.initials: K
authors.lastname: Ikebukuro
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Seimiya
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shin-Ya
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nagasawa
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D047028:Macrocyclic Compounds / Q000138:chemical synthesis; D008958:Models, Molecular; D009841:Oligonucleotides / Q000235:genetics; D010080:Oxazoles / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D008024:Ligands; D047028:Macrocyclic Compounds; D009841:Oligonucleotides; D010080:Oxazoles