Antioxidant activity and phenylpropanoids of Phlomis lychnitis L.: a traditional herbal tea.
Phlomis lychnitis L. (Lamiaceae) is consumed as a traditional herbal tea in Spain. The antioxidant-protective effects as well as its phytoconstituents have never been established. The ability of the methanolic extract to protect cells from oxidative stress was evaluated in rat pheochromocytoma cells (PC12) using hydrogen peroxide as toxic agent. The viability of PC12 cells pre-treated with the methanolic extract of Phlomis lychnitis, determined by the MTT and LDH assays, was significantly improved at the highest dose (p < 0.01). The antioxidant activity was confirmed evaluating the capacity of the plant to scavenge ABTS, DPPH, O(2) . (-) radicals and to inhibit XO. Bioassay guided fractionation led to antioxidant compounds. Qualitative HPLC/DAD/ESI/MS analysis reported phenylpropanoids, verbascoside being the major antioxidant constituent.
López
Víctor
V
Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Navarra, Irunlarrea sn, 31080, Pamplona, Spain. vlopezra@alumni.unav.es
Jäger
Anna K
AK
Akerreta
Silvia
S
Cavero
Rita Yolanda
RY
Calvo
Maria Isabel
MI
D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D005960:Glucosides / Q000032:analysis / Q000494:pharmacology*; D006861:Hydrogen Peroxide; D016716:PC12 Cells; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D031345:Phlomis / Q000737:chemistry*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D020005:Propanols / Q000032:analysis / Q000494:pharmacology*; D051381:Rats; D059808:Polyphenols
D000975:Antioxidants; D005960:Glucosides; D006861:Hydrogen Peroxide; D010636:Phenols; D010936:Plant Extracts; D020005:Propanols; C058956:acteoside; D059808:Polyphenols