Evaluation of cytochrome b mtDNA sequences in genetic diversity studies of Channa marulius (Channidae: Perciformes).
Channa marulius (Hamilton, 1822) is a commercially important freshwater fish and a potential candidate species for aquaculture. The present study evaluated partial Cytochrome b gene sequence of mtDNA for determining the genetic variation in wild populations of C. marulius. Genomic DNA extracted from C. marulius samples (n = 23) belonging to 3 distant rivers; Mahanadi, Teesta and Yamuna was analyzed. Sequencing of 307 bp Cytochrome b mtDNA fragment revealed the presence of 5 haplotypes with haplotype diversity value of 0.763 and nucleotide diversity value of 0.0128. Single population specific haplotype was observed in Mahanadi and Yamuna samples and 3 haplotypes in Teesta samples. The analysis of data demonstrated the suitability of partial Cytochrome b sequence in determining the genetic diversity in C. marulius population.
Habib
Maria
M
National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, UP, 226002, India.
Lakra
W S
WS
Mohindra
Vindhya
V
Khare
Praveen
P
Barman
A S
AS
Singh
Akanksha
A
Lal
Kuldeep K
KK
Punia
Peyush
P
Khan
Asif A
AA
D000818:Animals; D045303:Cytochromes b / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes; D008928:Mitochondria / Q000235:genetics; D010473:Perciformes / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial; D045303:Cytochromes b