title: Proteolytic activity in Fasciola hepatica is reduced by the administration of cathepsin L mimotopes.
abstract: The objective of this study was to assess the proteolytic activity of Fasciola hepatica cathepsins in liver sections from mice vaccinated with phage clones of cathepsin L mimotopes, using the film in situ zymography technique. Female BALB/c mice were immunized three times with 2.5 x 10¹¹ phage particles without adjuvant. Animals vaccinated with phage clones produced high titres of anti-mimotope antibodies and a significant reduction in fluke burden was observed following challenge with metacercariae of F. hepatica. The proteolytic activity in hepatic tissue was reduced after the immunization with phage clones.
authors.affiliation: Laboratorio de Genética y Reproducción, Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, 4 Sur 304 Col. Centro, C.P. 75482, Tecamachalco Puebla, México. abel.villa@gmail.com
authors.forename: A
authors.initials: A
authors.lastname: Villa-Mancera
authors.forename: H
authors.initials: H
authors.lastname: Quiroz-Romero
authors.forename: D
authors.initials: D
authors.lastname: Correa
authors.forename: R A
authors.initials: RA
authors.lastname: Alonso
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D017104:Bacteriophage M13 / Q000235:genetics / Q000276:immunology; D056668:Cathepsin L / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005210:Fasciola hepatica / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D005260:Female; D007114:Immunization; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D019151:Peptide Library; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000939:Epitopes; D019151:Peptide Library; D014612:Vaccines; D056668:Cathepsin L