title: A revisit to the one form kinetic model of prothrombinase.
abstract: Thrombin is generated enzymatically from prothrombin by two pathways with the intermediates of meizothrombin and prethrombin-2. Experimental concentration profiles from two independent groups for these two pathways have been re-analyzed. By rationally combining the independent data sets, a simple mechanism can be established and rate constants determined. A structural model is consistent with the data-derived finding that mechanisms that feature channeling or ratcheting are not necessary to describe thrombin production.
authors.affiliation: Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599-3290, USA.
authors.forename: Chang Jun
authors.initials: CJ
authors.lastname: Lee
authors.forename: Sangwook
authors.initials: S
authors.lastname: Wu
authors.forename: Changsun
authors.initials: C
authors.lastname: Eun
authors.forename: Lee G
authors.initials: LG
authors.lastname: Pedersen
mesh_terms: D001777:Blood Coagulation; D020134:Catalytic Domain; D007700:Kinetics; D056004:Molecular Dynamics Simulation; D017434:Protein Structure, Tertiary; D011516:Prothrombin / Q000737:chemistry / Q000378:metabolism; D013917:Thrombin / Q000737:chemistry / Q000378:metabolism; D013925:Thromboplastin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011516:Prothrombin; D013925:Thromboplastin; D013917:Thrombin