title: Kif26b, a kinesin family gene, regulates adhesion of the embryonic kidney mesenchyme.
abstract: The kidney develops through reciprocal interactions between two precursor tissues: the metanephric mesenchyme and the ureteric bud. We previously demonstrated that the zinc finger protein Sall1 is essential for ureteric bud attraction toward the mesenchyme. Here, we show that Kif26b, a kinesin family gene, is a downstream target of Sall1 and that disruption of this gene causes kidney agenesis because of impaired ureteric bud attraction. In the Kif26b-null metanephros, compact adhesion between mesenchymal cells adjacent to the ureteric buds and the polarized distribution of integrin alpha8 were impaired, resulting in failed maintenance of Gdnf, a critical ureteric bud attractant. Overexpression of Kif26b in vitro caused increased cell adhesion through interactions with nonmuscle myosin. Thus, Kif26b is essential for kidney development because it regulates the adhesion of mesenchymal cells in contact with ureteric buds.
authors.affiliation: Department of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 860-0811, Japan.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Uchiyama
authors.forename: Masaji
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Terabayashi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Inenaga
authors.forename: Shuji
authors.initials: S
authors.lastname: Inoue
authors.forename: Chiyoko
authors.initials: C
authors.lastname: Kobayashi
authors.forename: Naoko
authors.initials: N
authors.lastname: Oshima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyonari
authors.forename: Naomi
authors.initials: N
authors.lastname: Nakagata
authors.forename: Yuya
authors.initials: Y
authors.lastname: Sato
authors.forename: Kiyotoshi
authors.initials: K
authors.lastname: Sekiguchi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Miki
authors.forename: Eiichi
authors.initials: E
authors.lastname: Araki
authors.forename: Sayoko
authors.initials: S
authors.lastname: Fujimura
authors.forename: Satomi S
authors.initials: SS
authors.lastname: Tanaka
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Nishinakamura
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002448:Cell Adhesion / Q000502:physiology*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005260:Female; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007668:Kidney / Q000166:cytology / Q000196:embryology*; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D008648:Mesoderm / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014162:Transfection
chemical_list: D017931:DNA Primers; C487946:KIF26B protein, mouse; D016547:Kinesins