[Preparation of monoclonal antibodies against HN protein of Class I Newcastle disease virus virulent strain].
OBJECTIVE
To prepare monoclonal antibodies (mAb) against HN protein of Class I Newcaslte disease virus.


METHODS
Several 6-week-old mice were immunized with ClassI 9a5b virulent strain. Hemagglutination inhibition (HI), indirect immunofluorescence assay (IFA), cell neutralization test, ELISA and Western blot analysis were used to characterize and identify these antibodies.


RESULTS
Four hybridoma cell lines were successfully prepared, designated as mAb 2F5, 2F12, 3H7 and 3H9. Among them, only mAb 3H7 and 3H9 recognized HN protein of the ClassI strain, specifically neutralizing NDV and inhibiting hemagglutination.


CONCLUSIONS
mAb 3H7 and 3H9 could be of use in identification of Class I and Class II strains, and in functional studies of HN and cell receptors for NDV.
Sun
Ying-jie
YJ
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
Chen
Hong-jun
HJ
Song
Cui-ping
CP
Yu
Yang
Y
Qiu
Xu-sheng
XS
Yu
Sheng-qing
SQ
Ding
Chan
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015707:HN Protein / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D009522:Newcastle disease virus / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*
D000911:Antibodies, Monoclonal; D015707:HN Protein