Preliminary quantitative proteomic characterization of glaucomatous rat retinal ganglion cells.
Quantitative proteomic analysis was pursued of retinal ganglion cells (RGCs) from rats with unilateral experimental glaucoma. RGCs were isolated from 22 animals by immunopanning after 8 weeks of sustained elevated intraocular pressure. Proteins were quantified by LC MS/MS iTRAQ technology. Of the 268 proteins quantified, approximately 8% appeared elevated and approximately 13% decreased in glaucomatous RGCs. Voltage-dependent anion channel protein 2, aldose reductase, and ubiquitin were among the significantly elevated proteins while prothymosin was among the significantly decreased. The results demonstrate the feasibility of identifying global proteomic differences in protein expression between purified glaucomatous and control in vivo RGCs.
Crabb
John W
JW
Cole Eye Institute i31, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA. crabbj@ccf.org
Yuan
Xianglin
X
Dvoriantchikova
Galina
G
Ivanov
Dmitry
D
Crabb
John S
JS
Shestopalov
Valery I
VI
D000818:Animals; D002469:Cell Separation; D002853:Chromatography, Liquid; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000378:metabolism*; D005901:Glaucoma / Q000378:metabolism*; D007429:Intraocular Pressure; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014065:Tonometry, Ocular
D005136:Eye Proteins; D020543:Proteome