title: The regulation of jejunal induction of the maltase-glucoamylase gene by a high-starch/low-fat diet in mice.
abstract: Maltase and glucoamylase are derived from the same mRNA and are responsible for digestion of starch in the small intestine. Their jejunal activities in rodents are induced by a high-starch/low-fat (HS)-diet. However, it is unknown whether jejunal expression of the maltase-glucoamylase (Mgam) gene is enhanced by the HS-diet. In this study, we found that jejunal Mgam mRNA was increased by a HS-diet in mice. We showed that the HS-diet increased acetylation of histones, bindings of a coactivator, Creb binding protein (CREBBP), and the transcriptional factors caudal type homeobox 2 (CDX2) and HNF1 homeobox (HNF1) in the promoter/enhancer and transcriptional regions of Mgam gene. This suggests that the increase in the jejunal activity of maltase and glucoamylase caused by a HS-diet in mice is regulated at the mRNA level through histone acetylation and binding of CREBBP, CDX2 and HNF1 in the promoter/enhancer and transcriptional regions of Mgam gene.
authors.affiliation: Laboratory of Nutritional Physiology, The University of Shizuoka, Graduate School of Nutritional and Environmental Sciences and Global COE, Shizuoka, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Mochizuki
authors.forename: Kazue
authors.initials: K
authors.lastname: Honma
authors.forename: Masaya
authors.initials: M
authors.lastname: Shimada
authors.forename: Toshinao
authors.initials: T
authors.lastname: Goda
mesh_terms: D000107:Acetylation; D000818:Animals; D000071616:CDX2 Transcription Factor; D050882:CREB-Binding Protein / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004032:Diet / Q000009:adverse effects; D018752:Diet, Fat-Restricted; D004790:Enzyme Induction; D051537:Hepatocyte Nuclear Factor 1 / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism; D007583:Jejunum / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013213:Starch / Q000008:administration & dosage* / Q000009:adverse effects; D014157:Transcription Factors / Q000378:metabolism; D000520:alpha-Glucosidases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000071616:CDX2 Transcription Factor; C487514:Cdx2 protein, mouse; D004268:DNA-Binding Proteins; D006657:Histones; D018398:Homeodomain Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D051537:Hepatocyte Nuclear Factor 1; D013213:Starch; D050882:CREB-Binding Protein; C494364:Crebbp protein, mouse; D000520:alpha-Glucosidases