Dielectric barrier discharge plasma as a novel approach for improving 1,3-propanediol production in Klebsiella pneumoniae.
Dielectric barrier discharge plasma was used to generate a stable strain of Klebsiella pneumoniae (designated to as Kp-M2) with improved 1,3-propanediol production. The specific activities of glycerol dehydrogenase, glycerol dehydratase and 1,3-propanediol oxidoreductase in the crude cell extract increased from 0.11, 9.2 and 0.15 U mg(-1), respectively, for wild type to 0.67, 14.4 and 1.6 U mg(-1) for Kp-M2. The glycerol flux of Kp-M2 was redistributed with the flux to the reductive pathway being increased by 20% in batch fermentation. The final 1,3-propanediol concentrations achieved by Kp-M2 in batch and fed-batch fermentations were 19.9 and 76.7 g l(-1), respectively, which were higher than those of wild type (16.2 and 49.2 g l(-1)). The results suggested that dielectric barrier discharge plasma could be used as an effective approach to improve 1,3-propanediol production in K. pneumoniae.
Dong
Xiao-Yu
XY
School of Life Science and Biotechnology, Dalian University of Technology, Linggong Road 2, Dalian 116024, People's Republic of China.
Xiu
Zhi-Long
ZL
Li
Shuang
S
Hou
Ying-Min
YM
Zhang
Dai-Jia
DJ
Ren
Chun-Sheng
CS
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D003593:Cytoplasm / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D010949:Plasma / Q000378:metabolism*; D011409:Propylene Glycols / Q000378:metabolism*; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism
D001426:Bacterial Proteins; D003470:Culture Media; D011409:Propylene Glycols; C041787:1,3-propanediol; D013401:Sugar Alcohol Dehydrogenases; D000426:Alcohol Dehydrogenase; C052370:1,3-propanediol dehydrogenase; C020672:glycerol dehydrogenase; D006836:Hydro-Lyases; C020671:glycerol dehydratase; D005990:Glycerol