RNA-based investigation of ammonia-oxidizing archaea in hot springs of Yunnan Province, China.
Using RNA-based techniques and hot spring samples collected from Yunnan Province, China, we show that the amoA gene of aerobic ammonia-oxidizing archaea can be transcribed at temperatures higher than 74 degrees C and up to 94 degrees C, suggesting that archaeal nitrification can potentially occur at near boiling temperatures.
Jiang
Hongchen
H
State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences, Beijing, Geomicrobiology Laboratory, Beijing 100083, China. jianghc@cugb.edu.cn
Huang
Qiuyuan
Q
Dong
Hailiang
H
Wang
Peng
P
Wang
Fengping
F
Li
Wenjun
W
Zhang
Chuanlun
C
D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D002681:China; D045482:Hot Springs / Q000737:chemistry / Q000382:microbiology*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D019642:RNA, Archaeal / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D019843:Archaeal Proteins; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D000641:Ammonia; D010088:Oxidoreductases; C045582:ammonia monooxygenase