title: The in vitro biocompatibility of self-assembled hyperbranched copolyphosphate nanocarriers.
abstract: Polymeric micelles are recognized as very promising nanocarriers for small-molecule drugs, DNA, and proteins delivery. Polyphosphates are an important class of eminent biomaterials and widely used in biomedicine due to their good biocompatibility, biodegradability, and flexibility in adjusting the pendant structures. Here, some full-polyphosphate nanocarriers have been constructed successfully through self-assembly of amphiphilic hyperbranched multiarm copolymers (denoted as HPHEEP-star-PPEPs). The hydrophilic core and hydrophobic multiarm of HPHEEP-star-PPEPs are composed by hyperbranched and linear polyphosphates respectively. HPHEEP-star-PPEPs can self-assembly into nanocarriers in aqueous media with controlled size from 48 to 74 nm by conveniently adjusting the length of hydrophobic arm. These nanocarriers possess excellent biocompatibility against NIH 3T3 cells and are easily internalized by vivid cells. Chlorambucil-loaded nanocarriers were investigated for proliferation inhibition of an MDA-MB-231 breast cancer cell line in vitro, and the chlorambucil dose required for 50% cellular growth inhibition was found to be 3 microg/mL.
authors.affiliation: School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, PR China.
authors.forename: Jinyao
authors.initials: J
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Pang
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Zhu
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Zhou
authors.forename: Deyue
authors.initials: D
authors.lastname: Yan
mesh_terms: D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000737:chemistry / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D002699:Chlorambucil / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008823:Micelles; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D010710:Phosphates / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism
chemical_list: D018906:Antineoplastic Agents, Alkylating; D001672:Biocompatible Materials; D004337:Drug Carriers; D008823:Micelles; D010710:Phosphates; D011108:Polymers; D002699:Chlorambucil