title: Alterations in differentially expressed genes in the head of Oryzias latipes following benzo[a]pyrene exposure.
abstract: Differentially expressed genes (DEGs) in the head of medaka (Oryzias latipes) were investigated to assess the biological effects of benzo[a]pyrene (BaP) on genomic alterations. Eight and ten DEGs were identified in response to short (48 h) and long-term (15 days) exposure to BaP (25 microg/L), respectively, using annealing control primer-based polymerase chain reaction. Among the DEGs, matrilin 1 and prothrombin were commonly detected, suggesting that BaP exposure can alter the expression of genes associated with the formation of extracellular matrix and blood coagulation in the head of medaka.
authors.affiliation: Department of Biological Sciences, Pusan National University, Pusan 609-735, Korea.
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Oh
authors.forename: Hyo-Bang
authors.initials: HB
authors.lastname: Moon
authors.forename: Eun Sang
authors.initials: ES
authors.lastname: Choe
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D005880:Gills; D006257:Head; D009990:Oryzias / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D012313:RNA