title: [Expression of protein arginine N-methyltransferases in E3 rat models of acute asthma].
abstract: OBJECTIVE
  To observe the expression of protein arginine N-methyltransferase (PRMT) genes in the lung and spleen of E3 rats with acute asthma.
  
  
  METHODS
  E3 rats with ovalbumin-induced pulmonary inflammation were divided into two groups (n=10), and the validity of the acute asthma model was evaluated by histological observation with HE and PAS staining and by measurement of NO production. Semi-quantitative RT-PCR was employed to detect the expressions of PRMT1-PRMT6 genes in the lung and spleen tissues of the rats.
  
  
  RESULTS
  In the lung tissue of the asthmatic rats, the gene expressions of PRMT1 (P<0.01), PRMT2 (P<0.01), PRMT3 (P<0.05) and PRMT5 (P<0.05) were significantly increased, but the expression of PRMT4 gene (P<0.05) was significantly decreased as compared with those in the control tissue. In the spleen tissue of the asthmatic rats, the expressions of PRMT2 (P<0.05) and PRMT5 genes (P<0.05) showed a significant increase as compared with those in the control rat tissue.
  
  
  CONCLUSIONS
  The gene expressions of PRMTs vary significantly between asthmatic rats and control rats, suggesting that PRMTs play an important role in the post-translational modification process of asthma-related genes.
authors.affiliation: Department of Medical Genetics and Molecular Biology, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China.
authors.forename: Qing-zhu
authors.initials: QZ
authors.lastname: Sun
authors.forename: Fang-fang
authors.initials: FF
authors.lastname: Jiao
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhong
authors.forename: Mei-hua
authors.initials: MH
authors.lastname: Jiang
authors.forename: Guo-liang
authors.initials: GL
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Lü
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
authors.forename: Qi-lan
authors.initials: QL
authors.lastname: Ning
authors.forename: Fu-jun
authors.initials: FJ
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: She-min
authors.initials: SM
authors.lastname: Lü
mesh_terms: D000208:Acute Disease; D000818:Animals; D001249:Asthma / Q000201:enzymology*; D005260:Female; D008297:Male; D011499:Protein Processing, Post-Translational; D011484:Protein-Arginine N-Methyltransferases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011484:Protein-Arginine N-Methyltransferases