title: Minigenome-based reporter system suitable for high-throughput screening of compounds able to inhibit Ebolavirus replication and/or transcription.
abstract: We describe an Ebolavirus minigenome-based system that is suitable for high-throughput screening of compounds able to impair Ebolavirus virus replication and/or transcription. The assay is robust (Z' factor, >0.6) and can be carried out in low-biosafety containment. Results from a pilot screen of 960 compounds are presented.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA.
authors.forename: Luke D
authors.initials: LD
authors.lastname: Jasenosky
authors.forename: Gabriele
authors.initials: G
authors.lastname: Neumann
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D029043:Ebolavirus / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D014158:Transcription, Genetic / Q000187:drug effects* / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects* / Q000235:genetics
chemical_list: D000998:Antiviral Agents