title: The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration.
abstract: The RNA binding protein Larp1 was originally shown to be involved in spermatogenesis, embryogenesis and cell-cycle progression in Drosophila. Our data show that mammalian Larp1 is found in a complex with poly A binding protein and eukaryote initiation factor 4E and is associated with 60S and 80S ribosomal subunits. A reduction in Larp1 expression by siRNA inhibits global protein synthesis rates and results in mitotic arrest and delayed cell migration. Consistent with these data we show that Larp1 protein is present at the leading edge of migrating cells and interacts directly with cytoskeletal components. Taken together, these data suggest a role for Larp1 in facilitating the synthesis of proteins required for cellular remodelling and migration.
authors.affiliation: Department of Molecular Oncology, Imperial College, Hammersmith Campus, Du Cane Road, London W12 0HS, UK.
authors.forename: Carla
authors.initials: C
authors.lastname: Burrows
authors.forename: Normala
authors.initials: N
authors.lastname: Abd Latip
authors.forename: Sarah-Jane
authors.initials: SJ
authors.lastname: Lam
authors.forename: Lee
authors.initials: L
authors.lastname: Carpenter
authors.forename: Kirsty
authors.initials: K
authors.lastname: Sawicka
authors.forename: George
authors.initials: G
authors.lastname: Tzolovsky
authors.forename: Hani
authors.initials: H
authors.lastname: Gabra
authors.forename: Martin
authors.initials: M
authors.lastname: Bushell
authors.forename: David M
authors.initials: DM
authors.lastname: Glover
authors.forename: Anne E
authors.initials: AE
authors.lastname: Willis
authors.forename: Sarah P
authors.initials: SP
authors.lastname: Blagden
mesh_terms: D000199:Actins / Q000032:analysis; D017209:Apoptosis; D001324:Autoantigens / Q000378:metabolism / Q000502:physiology*; D002465:Cell Movement; D003598:Cytoskeletal Proteins / Q000378:metabolism; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D010442:Peptide Chain Initiation, Translational; D039102:Poly(A)-Binding Proteins / Q000378:metabolism; D011554:Pseudopodia / Q000737:chemistry / Q000648:ultrastructure; D012261:Ribonucleoproteins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D000097585:SS-B Antigen
chemical_list: D000199:Actins; D001324:Autoantigens; D003598:Cytoskeletal Proteins; D039561:Eukaryotic Initiation Factor-4E; D039102:Poly(A)-Binding Proteins; D012261:Ribonucleoproteins; D000097585:SS-B Antigen