Thermodynamic investigation of the binding of dissymmetric pyrenyl-gemini surfactants to DNA.
Gemini surfactants have demonstrated significant potential for use in constructing non-viral transfection vectors for the delivery of genes into cells to induce protein expression. Previously, two asymmetric gemini surfactants containing pyrenyl groups in one of the alkyl tails of the surfactants were synthesized as fluorescence probes for use in mechanistic studies of the transfection process. Here we present the results of a thermodynamic investigation of the binding interaction(s) between the pyrenyl-modified surfactants and DNA. The thermodynamics of the interactions have been examined using isothermal titration calorimetry, light scattering, zeta potential, and circular dichroism measurements. Distinct differences are observed between the interaction of 12-s-12 vs. the pyrene modified py-s-12 surfactants with DNA; an intercalated binding is found for the py-s-12 surfactants that disrupts the typical interactions observed between DNA and gemini surfactants.
Wettig
Shawn D
SD
School of Pharmacy, University of Waterloo, 200 University Ave. W., Waterloo, ON, N2L 3G1, Canada. wettig@uwaterloo.ca
Deubry
Rubena
R
Akbar
Javed
J
Kaur
Tranum
T
Wang
Haitang
H
Sheinin
Tatiana
T
Joseph
Jamie W
JW
Slavcev
Roderick A
RA
D000818:Animals; D002151:Calorimetry; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D012474:Salmon; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism*; D013816:Thermodynamics
D013501:Surface-Active Agents; D004247:DNA