Development of in situ hybridization for the detection of Mycoplasma hyorhinis in formalin-fixed paraffin-embedded tissues from naturally infected pigs with polyserositis.
The aim of this study was to develop in situ hybridization for detection of Mycoplasma hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from pigs with polyserositis. M. hyorhinis was isolated from the spleen (2 pigs) and pericardium (1 pig). M. hyorhinis DNA was detected 16 out of 20 pigs with polyserositis. In situ hybridization produced a distinct positive signal for the M. hyorhinis p37 gene in inflammatory cells in the polyserositis. In situ hybridization developed in the present study present diagnostic tools capable of detection of M. hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from the naturally infected pigs.
Kim
Bongtae
B
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Gwanak-Gu, Seoul, Republic of Korea.
Lee
Kichan
K
Han
Kiwon
K
Kim
Duyeol
D
Ha
Yooncheol
Y
Kim
Chung Hyun
CH
Oh
Yeonsu
Y
Kang
Ikjae
I
Lee
Jeehoon
J
Chae
Chanhee
C
D000818:Animals; D020029:Base Pairing; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification; D006321:Heart / Q000382:microbiology; D017403:In Situ Hybridization / Q000662:veterinary; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D045790:Mycoplasma hyorhinis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012700:Serositis / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology*
D017931:DNA Primers; D004269:DNA, Bacterial