Light microscopic, electron microscopic, and immunohistochemical comparison of Bama minipig (Sus scrofa domestica) and human skin.
Here we sought to evaluate the possibility of using Chinese Bama miniature pig skin as a suitable animal model for human skin. Morphologic features of the skin of Bama miniature pigs resemble those of human skin, including skin layer thickness, development of a superficial vascular system, structure of the dermal-epidermal interface, and extracellular matrix. The characteristics and densities of Langerhans cells, fibroblasts, vascular endothelial cells, and mast cells were similar between Bama pig and human skin. Immunohistochemistry showed that miniature pigs and humans have the same antigenic determinants of human laminin, fibronectin, filaggrin, collagen I, collagen III, collagen IV, and keratin but not CD34, ICAM1, or S100. In addition, collagen type I from Bama miniature pig skin exhibited physicochemical characteristics resembling those of human skin, in regard to HPLC chromatography, UV spectroscopy, amino-acid composition, and SDS-PAGE analysis. Given these results, we concluded that Bama miniature pigs have great potential as a human skin model and for developing dermal substitute materials in wound repair. However, we also observed some disparities between the skin of Bama miniature pigs and humans, including pigment cell distribution, sweat gland types, and others. Therefore, further studies are needed to completely evaluate the effects of these interspecies differences on the actual application of the model.
Liu
Yu
Y
Department of Laboratory Animal Science, College of Basic Medicine, Third Military Medical University, Chongqing, China.
Chen
Jun-ying
JY
Shang
Hai-tao
HT
Liu
Chang-e
CE
Wang
Yong
Y
Niu
Rong
R
Wu
Jun
J
Wei
Hong
H
D000818:Animals; D024042:Collagen Type I / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005260:Female; D000091344:Filaggrin Proteins; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008853:Microscopy / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods; D023421:Models, Animal; D012867:Skin / Q000737:chemistry* / Q000648:ultrastructure*; D034421:Sus scrofa; D013552:Swine; D013556:Swine, Miniature / Q000033:anatomy & histology* / Q000378:metabolism; D014945:Wound Healing
D024042:Collagen Type I; C000717249:FLG protein, human; D000091344:Filaggrin Proteins