An event-specific DNA microarray to identify genetically modified organisms in processed foods.
We developed an event-specific DNA microarray system to identify 19 genetically modified organisms (GMOs), including two GM soybeans (GTS-40-3-2 and A2704-12), thirteen GM maizes (Bt176, Bt11, MON810, MON863, NK603, GA21, T25, TC1507, Bt10, DAS59122-7, TC6275, MIR604, and LY038), three GM canolas (GT73, MS8xRF3, and T45), and one GM cotton (LLcotton25). The microarray included 27 oligonucleotide probes optimized to identify endogenous reference targets, event-specific targets, screening targets (35S promoter and nos terminator), and an internal target (18S rRNA gene). Thirty-seven maize-containing food products purchased from South Korean and US markets were tested for the presence of GM maize using this microarray system. Thirteen GM maize events were simultaneously detected using multiplex PCR coupled with microarray on a single chip, at a limit of detection of approximately 0.5%. Using the system described here, we detected GM maize in 11 of the 37 food samples tested. These results suggest that an event-specific DNA microarray system can reliably detect GMOs in processed foods.
Kim
Jae-Hwan
JH
Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
Kim
Su-Youn
SY
Lee
Hyungjae
H
Kim
Young-Rok
YR
Kim
Hae-Yeong
HY
D029741:Brassica rapa / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D003368:Gossypium / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013025:Glycine max / Q000235:genetics; D003313:Zea mays / Q000235:genetics
D018744:DNA, Plant; D015345:Oligonucleotide Probes