title: Genetic characterisation and structural analysis of the O-specific polysaccharide of Yersinia pseudotuberculosis serotype O:1c.
abstract: Many, but not all, of the current 21 serotypes of Yersinia pseudotuberculosis have been investigated with regard to the chemical structures of their O-specific polysaccharide (OPS) and the genetic basis of their biosynthesis. Completion of the genetics and structures of the remaining serotypes will enhance our understanding of the emerging relationship between genetics and structures within this species. Here, we present a structural and genetic analysis of the Y. pseudotuberculosis serotype O:1c OPS. Our results showed that this OPS has the same backbone as Y. pseudotuberculosis O:2b, but with a 3,6-dideoxy-D-ribo-hexofuranose (paratofuranose, Parf) side-branch instead of a 3,6-dideoxy-D-xylo-hexopyranose (abequopyranose, Abep). The 3'-end of the gene cluster is the same as for O:2b and has the genes for synthesis of the backbone and for processing the completed repeat unit. The 5'-end of the cluster consists of the same genes as O:1b for synthesis of Parf and a related gene for its transfer to the repeating unit backbone.
authors.affiliation: Department of Organic Chemistry and Biochemistry, University Federico II of Naples, Naples, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: De Castro
authors.forename: Johanna J
authors.initials: JJ
authors.lastname: Kenyon
authors.forename: Monica M
authors.initials: MM
authors.lastname: Cunneen
authors.forename: Peter R
authors.initials: PR
authors.lastname: Reeves
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
authors.forename: Otto
authors.initials: O
authors.lastname: Holst
authors.forename: Mikael
authors.initials: M
authors.lastname: Skurnik
mesh_terms: D002240:Carbohydrate Sequence; D054884:Host-Pathogen Interactions; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D019081:O Antigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017421:Sequence Analysis; D012698:Serologic Tests; D013045:Species Specificity; D015011:Yersinia pseudotuberculosis / Q000276:immunology*; D015012:Yersinia pseudotuberculosis Infections / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology*
chemical_list: D019081:O Antigens