title: WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro.
abstract: OBJECTIVE
  To investigate the mode of action of WSS45, one sulfated derivative of an alpha-D-glucan from the Gastrodia elata Bl, on the multiplication cycle of dengue virus serotype 2 (DV2), including initial infection and intracellular replication.
  
  
  METHODS
  Virus multiplication in BHK cells were monitored by qRT-PCR, plaque reduction assay, and flow cytometry. Initial virus infection was dissected into adsorption and penetration steps by converting temperature and treating by acid glycine. Surface bound virions were detected by immunofluorescence staining for Evelope protein.
  
  
  RESULTS
  WSS45 effectively inhibited DV2 infection in BHK cells with an EC(50) value of 0.68+/-0.17 microg/mL, mainly interfered with virus adsorption, in a very early stage of the virus cycle. However, WSS45 showed no viricidal effect. Moreover, WSS45 could increase the detaching of virus from cell surface in BHK cell line.
  
  
  CONCLUSIONS
  WSS45 exerted potent inhibitory effect on DV2 through interfering with the interaction between viruses and targeted cells. This activity was related to its molecular size.
authors.affiliation: Laboratory of Immunopharmacology and Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Xian-kun
authors.initials: XK
authors.lastname: Tong
authors.forename: Hong
authors.initials: H
authors.lastname: Qiu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Shi
authors.forename: Gui-feng
authors.initials: GF
authors.lastname: Wang
authors.forename: Fei-hong
authors.initials: FH
authors.lastname: Ji
authors.forename: Hui-yong
authors.initials: HY
authors.lastname: Ding
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Kan
authors.initials: K
authors.lastname: Ding
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Zuo
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003715:Dengue / Q000188:drug therapy*; D003716:Dengue Virus / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D031668:Gastrodia / Q000737:chemistry*; D005936:Glucans / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D005936:Glucans; C078962:glucan sulfate