[Effect of proteasome inhibitor MG-132 on L1210 cell apoptosis and its mechanism].
This study was aimed to investigate the effect of proteasome inhibitor MG-132 on apoptosis of L1210 cells and its mechanism. L1210 cells were treated with MG-132 of different concentrations (0, 2.5, 5, 10, 10 micromol/L). Cell viability was tested by MTT assay, apoptosis rate was detected by using flow cytometry, activity of caspase 3 was detected by colorimetry, the expression of NF-kappaB nuclear protein was detected by Western blot. The results showed that the growth inhibition of L1210 cells treated for a same time (24 hours) was enhanced along with increasing of MG-132 concentrations (0, 2.5, 5, 10, 20 micromol/L); the inhibitory rate, apoptosis rate and activity of caspase 3 increased also along with raising of MG-132 concentrations; while the expression of NF-kappaB nuclear protein decreased along with raising of GM-132 concentrations. It is concluded that MG-132 can induce the apoptosis of L1210. The mechanism of apoptosis may be related to the down-regulation of the expression of NF-kappaB and the activation of caspase 3.
Li
Xiao-Hua
XH
Department of Pediatrics, Xuzhou Medical College Affiliated Hospital, Xuzhou 221002, Jiangsu Province, China.
Xue
Tian-Yang
TY
Xu
Wei
W
Gao
Ji-Zhao
JZ
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D007976:Leupeptins / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism
D004791:Enzyme Inhibitors; D007976:Leupeptins; D016328:NF-kappa B; C505407:CASP3 protein, human; D053148:Caspase 3; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde