Cohaesibacter marisflavi sp. nov., isolated from sediment of a seawater pond used for sea cucumber culture, and emended description of the genus Cohaesibacter.
A Gram-negative, catalase-negative, oxidase-positive, rod-shaped bacterium, strain DQHS21(T), was isolated from sediment of a seawater pond used for sea cucumber culture at Jimo in Qingdao province on the east coast of China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DQHS21(T) belonged to the genus Cohaesibacter, sharing the highest sequence similarity (96.1 %) with Cohaesibacter gelatinilyticus CL-GR15(T), while the similarity to other strains was below 93.0 %. The cellular fatty acids consisted mainly of C(18 : 1)ω7c (60.7 %), C(18 : 0) (17.8 %), C(16 : 0) (8.5 %) and summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH; 6.0 %), which together accounted for 93 % of the total fatty acids. Ubiquinone 10 was the major quinone. The G+C content of the chromosomal DNA of strain DQHS21(T) was 55.2 mol%. The combined genotypic and phenotypic data showed that strain DQHS21(T) represents a novel species of the genus Cohaesibacter, for which the name Cohaesibacter marisflavi sp. nov. is proposed, with the type strain DQHS21(T) ( = CGMCC 1.9157(T)  = NCCB 100300(T)).
Qu
Lingyun
L
First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, PR China.
Lai
Qiliang
Q
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
Zhu
Fengling
F
First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, PR China.
Hong
Xuguang
X
First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, PR China.
Sun
Xiuqin
X
First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, PR China.
Shao
Zongze
Z
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000818:Animals; D017756:Aquaculture; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012616:Sea Cucumbers / Q000254:growth & development; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; D002374:Catalase