Bifurcate effects of glucose on caspase-independent cell death during hypoxia.
We investigated the effect of glucose on hypoxic death of rat cardiomyocyte-derived H9c2 cells and found that there is an optimal glucose concentration for protection against hypoxic cell death. Hypoxic cell death in the absence of glucose is accompanied by rapid ATP depletion, release of apoptosis-inducing factor from mitochondria, and nuclear chromatin condensation, all of which are inhibited by glucose in a dose-dependent manner. In contrast, excessive glucose also induces hypoxic cell death that is not accompanied by these events, suggesting a change in the mode of cell death between hypoxic cells with and without glucose supplementation.
Aki
Toshihiko
T
Section of Forensic Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan. aki.legm@tmd.ac.jp
Nara
Akina
A
Funakoshi
Takeshi
T
Uemura
Koichi
K
D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism; D015687:Cell Hypoxia / Q000187:drug effects; D002460:Cell Line; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D000860:Hypoxia / Q000378:metabolism* / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000473:pathology; D051381:Rats
D020169:Caspases; D005947:Glucose