Deep-tissue imaging of intramolecular fluorescence resonance energy-transfer parameters.
We demonstrate the in vivo reconstruction of all fluorescence resonance energy transfer (FRET) parameters, including the nanometer donor-acceptor distance, in a mouse. The FRET chemical targets cancer cells, and on internalization, the acceptor is released, in lieu of a targeted anticancer drug in chemotherapy. Our method provides a new vehicle for studying disease by imaging FRET parameters in deep tissue.
Gaind
Vaibhav
V
School of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Kularatne
Sumith
S
Low
Philip S
PS
Webb
Kevin J
KJ
D000818:Animals; D004867:Equipment Design; D005260:Female; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation; D051379:Mice; D008819:Mice, Nude; D012878:Skin Neoplasms / Q000378:metabolism*