The protective effects of osmolytes on yeast alcohol dehydrogenase conformational stability and aggregation.
The protective effects of four osmolytes (trehalose, dimethysulfoxide, glycine and proline) on the conformational stability and aggregation of guanidine-denatured yeast alcohol dehydrogenase (YADH) have been investigated in this paper. The results show that the four osmolytes protect YADH against unfolding and inactivation by reducing ki (inactivation rate constants), increasing DeltaDeltaGi (transition free energy changes at 25 degrees C), increasing Cm (value for the midpoint of denaturation) and decreasing its ANS-binding fluorescence intensity. Furthermore, these osmolytes can prevent YADH aggregation in a concentration-dependent manner during YADH refolding.
Han
Hong-Yan
HY
Department of Biology, Medical College of Soochow University, Jiangsu, Suzhou 215123, PR China.
Yao
Zhi-Gang
ZG
Gong
Cheng-Liang
CL
Xu
Wei-An
WA
D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D004789:Enzyme Activation; D009997:Osmotic Pressure; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity; D056811:Unfolded Protein Response
D000426:Alcohol Dehydrogenase