[Inhibitory effect of flavonoids of puerarin on proliferation of different human acute myeloid leukemia cell lines in vitro].
This study was aimed to investigate the inhibitory effect of flavonoids of puerarin (PR) in different concentrations on proliferation of 4 kinds of acute myeloid leukemia (AML) cell lines (Kasumi-1, HL-60, NB4 and U937), and to explore its possible mechanism. The MTT method was used to detected the inhibitory effect of PR on proliferation of AML cell lines. The flow cytometry was adopted to determine the change of cell cycle in vitro. The results showed that a certain concentration of PR could inhibit the proliferation of these 4 cell lines effectively in time-and dose-dependent manners, and the intensity of inhibition on 4 kinds of AML cell lines was from high to low as follows: NB4>Kasumi-1>U937>HL-60. Meanwhile, PR could also change cycle process, cell proportion in G1/G0 phase decreased, cells in S phase increased and Sub-diploid peak also appeared. It is concluded that PR can selectively inhibit the proliferation of 4 AML cell lines and block cell cycle process, especially for NB4 cells.
Shao
Hua-Min
HM
Nanjing University of Chinese Medicine, Department of Hematology, The First Affiliated Hospital, Nanjing 210046, Jiangsu Province, China.
Tang
Yu-Hong
YH
Jiang
Peng-Jun
PJ
Zhu
Hong-Qing
HQ
Zhang
Ya-Cheng
YC
Ji
Jian-Min
JM
Ji
Ou
O
Shen
Qun
Q
D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D015470:Leukemia, Myeloid, Acute / Q000145:classification / Q000473:pathology; D020298:U937 Cells
D007529:Isoflavones; C033607:puerarin