Light-induced root hair formation in lettuce (Lactuca sativa L. cv. Grand Rapids) roots at low pH is brought by chlorogenic acid synthesis and sugar.
Previously, we reported that chlorogenic acid (CGA) facilitated root hair formation at pH 4.0 in lettuce (Lactuca sativa L. cv. Grand Rapids). Light was essential for this process. In the present study, we determined relationships between CGA, light, and sugar during root hair formation in lettuce seedlings. The amount of CGA increased with white light in intact seedlings. Exogenously applied CGA restored root hair formation in dark-grown intact seedlings at pH 4.0. However, no root hair formation was induced in decapitated seedlings regardless of light exposure and CGA application. Application of sucrose or glucose induced both root hair formation and CGA synthesis in light-grown decapitated seedlings at pH 4.0. Blue light was the most effective for both root hair formation and CGA synthesis when supplied with sucrose to decapitated seedlings. Addition of sucrose and CGA together induced root hair formation at pH 4.0 in dark-grown decapitated seedlings. Results suggest that light induced CGA synthesis from sugar in the roots. Sugar was also required for root hair formation other than starting material of CGA synthesis. In addition, an unknown low pH-induced factor was essential for lettuce root hair formation.
Narukawa
Megumi
M
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
Watanabe
Keiji
K
Inoue
Yasunori
Y
D050260:Carbohydrate Metabolism / Q000187:drug effects / Q000528:radiation effects*; D002241:Carbohydrates / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000378:metabolism* / Q000494:pharmacology; D003624:Darkness; D006863:Hydrogen-Ion Concentration / Q000187:drug effects / Q000528:radiation effects; D018545:Lactuca / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects*; D008027:Light; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects
D002241:Carbohydrates; D002726:Chlorogenic Acid; C019145:alpha-aminooxy-beta-phenylpropionic acid; D010649:Phenylalanine