title: Effect of follicle diameter on oocyte apoptosis, embryo development and chromosomal ploidy in prepubertal goats.
abstract: The aim of this study was to assess the following parameters in prepubertal goat oocytes of different follicle diameter (> or =3 mm, <3 mm, control): oocyte diameter, early (Annexin-V) and late (TUNEL) apoptosis, embryo development and chromosomal ploidy of these blastocysts using Fluorescence In Situ Hybridization (FISH). Before in vitro maturation, oocytes were measured and stained with Annexin-V or TUNEL. The rest of the oocytes were matured, fertilized, and cultured in vitro for 8 days. Oocytes from follicles of > or =3 mm showed greater mean oocyte diameter (128.27 +/- 7.20 microm vs. 125.35 +/- 7.59 microm), higher percentages of TUNEL positive (42.86 vs. 24.23%), higher cleavage (47.85 +/- 3.98 vs. 23.07 +/- 2.44 %) and blastocyst rates (19.77 +/- 3.04 vs. 4.11 +/- 1.10 %) than oocytes from follicles of <3 mm.. Blastocyst mean cell numbers did not show differences between follicular groups (123.83 +/- 49.62 vs. 104.29 +/- 36.09 for follicles of > or =3 mm and <3 mm, respectively). A total of 54 blastocysts with 7084 nuclei were hybridized with specific probes to chromosomes X and Y. Ninety-eight percent (98%) of the embryos presented at least one cell carrying an abnormal number of chromosomes, but 78% of them presented less than 25% of chromosomal abnormal cells. No differences in the percentage of blastocysts with abnormal ploidy were found in embryos produced from oocytes of different follicle diameter.
authors.affiliation: Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Romaguera
authors.forename: A
authors.initials: A
authors.lastname: Casanovas
authors.forename: R
authors.initials: R
authors.lastname: Morató
authors.forename: D
authors.initials: D
authors.lastname: Izquierdo
authors.forename: M
authors.initials: M
authors.lastname: Catalá
authors.forename: A R
authors.initials: AR
authors.lastname: Jimenez-Macedo
authors.forename: T
authors.initials: T
authors.lastname: Mogas
authors.forename: M T
authors.initials: MT
authors.lastname: Paramio
mesh_terms: D000818:Animals; D017209:Apoptosis; D033481:Chromosomes, Mammalian; D004622:Embryo, Mammalian / Q000166:cytology; D047108:Embryonic Development; D005260:Female; D006041:Goats / Q000196:embryology* / Q000235:genetics / Q000254:growth & development; D009865:Oocytes / Q000166:cytology*; D006080:Ovarian Follicle / Q000033:anatomy & histology*; D011003:Ploidies; D012741:Sexual Maturation