title: Molecular cytogenetic characterization of stem-like cancer cells isolated from established cell lines.
abstract: There is indication that tumor growth is sustained by subpopulation of cells with stem-like features but little is known on their genomic characterization and their genetic stability. We report a detailed molecular cytogenetic characterization using Spectral Karyotyping and fluorescent in situ hybridization of parental serum-cultured adherent cells and their sphere-growing stem-like counterpart before and after differentiation from six cell lines established from solid tumors. Our findings indicate increased cytogenetic complexity in sphere-growing stem-like and their differentiated adherent cells compared to parental adherent component suggesting the existence within cell lines of heterogeneous and genetically unstable subpopulations of cells endowed with stem-like features.
authors.affiliation: Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Gasparini
authors.forename: Giulia
authors.initials: G
authors.lastname: Bertolini
authors.forename: Mara
authors.initials: M
authors.lastname: Binda
authors.forename: Alessandra
authors.initials: A
authors.lastname: Magnifico
authors.forename: Luisa
authors.initials: L
authors.lastname: Albano
authors.forename: Monica
authors.initials: M
authors.lastname: Tortoreto
authors.forename: Graziella
authors.initials: G
authors.lastname: Pratesi
authors.forename: Federica
authors.initials: F
authors.lastname: Facchinetti
authors.forename: Gabriella
authors.initials: G
authors.lastname: Abolafio
authors.forename: Luca
authors.initials: L
authors.lastname: Roz
authors.forename: Elda
authors.initials: E
authors.lastname: Tagliabue
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Daidone
authors.forename: Gabriella
authors.initials: G
authors.lastname: Sozzi
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002455:Cell Division; D045744:Cell Line, Tumor / Q000473:pathology*; D002869:Chromosome Aberrations; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping / Q000379:methods; D009369:Neoplasms / Q000235:genetics; D013234:Stem Cells / Q000473:pathology*
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor