title: [Localization of polypeptides release factors and ribosome protein L11 in Euplotes octocarinatus].
abstract: Protozoan ciliates are a group of unicellular eukaryotes. The special characteristics of stop codons usage in termination of protein biosynthesis in ciliates cells makes them an ideal model to study the mechanism of stop codon recognition of polypeptides release factors. To localize the functional positions of biomolecules in ciliates cell, we constructed a macronuclear artificial chromosome containing a gene encoding red fluorescence protein (EoMAC_R) based on the structural characteristics of ciliates chromosome. Three factors, L11, eRF1a, and eRF3 that are involved in termination process of protein synthesis were colocalized in Euplotes octocarinatus cells by using novel EoMAC_R and the previously constructed EoMAC_G. The results indicated that protein synthesis mainly occurred inside the "C" shape macronucleus, suggesting that EoMAC could be a useful tool for localizing biomolecules in ciliates cell.
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, China. bfchai@sxu.edu.cn
authors.forename: Baofeng
authors.initials: B
authors.lastname: Chai
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Jingtao
authors.initials: J
authors.lastname: Wang
authors.forename: Quan
authors.initials: Q
authors.lastname: Shen
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Zhang
authors.forename: Aihua
authors.initials: A
authors.lastname: Liang
mesh_terms: D022201:Chromosomes, Artificial; D018388:Codon, Terminator / Q000378:metabolism; D016812:Euplotes / Q000737:chemistry*; D010454:Peptide Termination Factors / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000235:genetics; D015800:Protozoan Proteins / Q000032:analysis / Q000235:genetics; D012269:Ribosomal Proteins / Q000032:analysis* / Q000235:genetics
chemical_list: D018388:Codon, Terminator; D010454:Peptide Termination Factors; D010455:Peptides; D015800:Protozoan Proteins; D012269:Ribosomal Proteins; C027624:ribosomal protein L11