title: Enhanced reactivity to malondialdehyde-modified proteins by systemic lupus erythematosus autoantibodies.
abstract: OBJECTIVE
  Evaluation of the reactivity of autoantibodies of systemic lupus erythematosus (SLE) patients directed against malondialdehyde (MDA)-modified catalase, superoxide dismutase (SOD), and different Hep2 protein fractions (hydrophobic, hydrophilic, and nuclear).
  
  
  METHODS
  Thiol groups and MDA-protein adducts were first assessed among 65 SLE patients and 60 healthy controls. Then, the reactivities of SLE immunoglobulin (Ig)G autoantibodies towards MDA-modified and unmodified proteins were compared using a standard enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  An increase in the levels of MDA-modified proteins and a decrease in the concentration of thiol groups among SLE patients (p < 0.05) were observed. IgG circulating autoantibodies in the sera of SLE patients exhibited a significant enhanced reactivity (p < 0.05) against catalase and SOD-modified proteins. The same data were observed in the different protein fractions extracted from cultured cells (p < 0.05).
  
  
  CONCLUSIONS
  These data reinforce the role of oxidative stress and especially lipid peroxidation products in the progression of SLE disease.
authors.affiliation: Unit of Biotechnology and Pathology, Higher Institute of Biotechnology of Sfax, Tunisia. riadhbm2004@yahoo.fr
authors.forename: R
authors.initials: R
authors.lastname: Ben Mansour
authors.forename: S
authors.initials: S
authors.lastname: Lassoued
authors.forename: A
authors.initials: A
authors.lastname: Elgaied
authors.forename: S
authors.initials: S
authors.lastname: Haddouk
authors.forename: S
authors.initials: S
authors.lastname: Marzouk
authors.forename: Z
authors.initials: Z
authors.lastname: Bahloul
authors.forename: H
authors.initials: H
authors.lastname: Masmoudi
authors.forename: H
authors.initials: H
authors.lastname: Attia
authors.forename: M S
authors.initials: MS
authors.lastname: Aïfa
authors.forename: F
authors.initials: F
authors.lastname: Fakhfakh
mesh_terms: D000704:Analysis of Variance; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology*; D008315:Malondialdehyde / Q000276:immunology*; D011506:Proteins / Q000276:immunology*; D013482:Superoxide Dismutase / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D011506:Proteins; D008315:Malondialdehyde; D013482:Superoxide Dismutase