title: Functional identification of a novel 14-3-3 epsilon splicing variant suggests dimerization is not necessary for 14-3-3 epsilon to inhibit UV-induced apoptosis.
abstract: 14-3-3 proteins function as a dimer and have been identified to involve in diverse signaling pathways. Here we reported the identification of a novel splicing variant of human 14-3-3 epsilon (14-3-3 epsilon sv), which is derived from a novel exon 1' insertion. The insertion contains a stop codon and leads to a truncated splicing variant of 14-3-3 epsilon. The splicing variant is translated from the exon 2 and results in the deletion of an N-terminal alpha-helix which is crucial for the dimerization. Therefore, the 14-3-3 epsilon sv could not form a dimer with 14-3-3 zeta. However, after UV irradiation 14-3-3 epsilon sv could also support cell survival, suggesting monomer of 14-3-3 epsilon is sufficient to protect cell from apoptosis.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China.
authors.forename: Dingding
authors.initials: D
authors.lastname: Han
authors.forename: Guangming
authors.initials: G
authors.lastname: Ye
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.forename: Cong
authors.initials: C
authors.lastname: Chen
authors.forename: Xianmei
authors.initials: X
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Wan
authors.forename: Yuanwang
authors.initials: Y
authors.lastname: Pan
authors.forename: Long
authors.initials: L
authors.lastname: Yu
mesh_terms: D048948:14-3-3 Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D017209:Apoptosis; D017124:Conserved Sequence; D005091:Exons / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D014466:Ultraviolet Rays
chemical_list: D048948:14-3-3 Proteins; C482983:YWHAE protein, human