title: Apheresis induces oxidative stress in blood cells.
abstract: Oxidative stress mediates damage to various cells and is thought to be involved in various pathologies, including hereditary and acquired hemolytic anemias. It is induced by a multitude of physiological and environmental factors, including extracorporeal manipulation of blood. As a result, hemodialysis induces oxidative damage to red blood cells, thereby increasing their susceptibility to hemolysis and shortening their life span. We studied the effect of apheresis on the oxidative status of blood components. Using flow cytometric measurements, we showed that red blood cells, lymphocytes, monocytes, and polymorphonuclear cells undergo oxidative stress induced by the procedure. Their reactive oxygen species and externalization of phosphatidylserine increased, while their levels of reduced glutathione decreased. This oxidative stress, which may be caused by a direct interaction with the membranous system, may lead to cellular abnormalities with clinical consequences such as hemolysis and platelet hyperactivation.
authors.affiliation: Department of Health Professions, Al-Quds University, Abu Dies, Jerusalem, Israel. johnnyamer@hotmail.com
authors.forename: Johnny
authors.initials: J
authors.lastname: Amer
authors.forename: Shoshana
authors.initials: S
authors.lastname: Frankenburg
authors.forename: Eitan
authors.initials: E
authors.lastname: Fibach
mesh_terms: D001773:Blood Cells; D001781:Blood Component Removal / Q000009:adverse effects* / Q000379:methods; D005434:Flow Cytometry; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D018384:Oxidative Stress; D010718:Phosphatidylserines / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D010718:Phosphatidylserines; D017382:Reactive Oxygen Species; D005978:Glutathione