title: Microtensile bond strength of etch-and-rinse and self-etch adhesive systems to demineralized dentin after the use of a papain-based chemomechanical method.
abstract: OBJECTIVE
  To evaluate the in vitro microtensile bond strength (microTBS) of etch-and-rinse and self-etch adhesive systems to demineralized dentin after the use of a papain-based chemomechanical method.
  
  
  METHODS
  36 demineralized human dentin slabs were randomly distributed into two groups according to the method of caries removal: (1). Mechanical removal with manual excavators; (2) Chemomechanical removal with a papain-based gel (Papacárie). Subsequently, three adhesive systems were applied (n=6): (a) an etch-and-rinse adhesive system (Single Bond); (b) a two-step self-etch adhesive system (AdheSE); (c) a one-step self-etch adhesive system (Adper Prompt). The slabs were restored with a microhybrid resin composite and each resin-dentin block was sectioned into 1.0 mm2 thick slabs, which were kept in receptacles containing distilled water at relative humidity, for 24 hours, at 37 degrees C. After that, they were subjected to tensile stress in a universal testing machine at a speed of 0.5 mm/minute. Data were submitted to two-way ANOVA and Tukey's test at a 0.05 level of significance. The fractured specimens were observed under a stereomicroscope to assess the failure mode.
  
  
  RESULTS
  The application of both chemomechanical and mechanical methods on demineralized dentin yielded microTBS values that were statistically similar among them, regardless of the adhesive system used. Caries removal with a chemomechanical papain-based method did not interfere in the adhesion of the tested adhesive systems to demineralized dentin.
authors.affiliation: Faculty of Dentistry and Dental Research Center São Leopoldo Mandic, Campinas, São Paulo, Brazil.
authors.forename: Renato José
authors.initials: RJ
authors.lastname: Gianini
authors.forename: Flávia Lucisano Botelho
authors.initials: FL
authors.lastname: do Amaral
authors.forename: Flávia Martão
authors.initials: FM
authors.lastname: Flório
authors.forename: Roberta Tarkany
authors.initials: RT
authors.lastname: Basting
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D000180:Acrylic Resins; D017438:Bisphenol A-Glycidyl Methacrylate; D001840:Dental Bonding; D003737:Dental Cavity Preparation / Q000379:methods; D003799:Dental Stress Analysis; D003804:Dentin / Q000473:pathology; D017220:Dentin-Bonding Agents; D006801:Humans; D008422:Materials Testing; D010206:Papain; D019279:Resin Cements; D013718:Tensile Strength; D017001:Tooth Demineralization
chemical_list: D000180:Acrylic Resins; C489662:AdheSE cement; D017220:Dentin-Bonding Agents; C509557:Papacarie; C408584:Prompt L-Pop; D019279:Resin Cements; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; D010206:Papain