title: Magnetic nanoparticles for imaging dendritic cells.
abstract: We report the development of superparamagnetic iron oxide (SPIOs) nanoparticles and investigate the migration of SPIO-labeled dendritic cells (DCs) in a syngeneic mouse model using magnetic resonance (MR) imaging. The size of the dextran-coated SPIO is roughly 30 nm, and the DCs are capable of independent uptake of these particles, although not at levels comparable to particle uptake in the presence of a transfecting reagent. On average, with the assistance of polylysine, the particles were efficiently delivered inside DCs within one hour of incubation. The SPIO particles occupy approximately 0.35% of cell surface and are equivalent to 34.6 pg of iron per cell. In vivo imaging demonstrated that the labeled cells migrated from the injection site in the footpad to the corresponding popliteal lymph node. The homing of labeled cells in the lymph nodes resulted in a signal drop of up to 79%. Furthermore, labeling DCs with SPIO particles did not compromise cell function, we demonstrated that SPIO-enhanced MR imaging can be used to track the migration of DCs effectively in vivo.
authors.affiliation: Vanderbilt University, Institute of Imaging Science, Nashville, Tennessee 37232-2310, USA.
authors.forename: Saho
authors.initials: S
authors.lastname: Kobukai
authors.forename: Richard
authors.initials: R
authors.lastname: Baheza
authors.forename: Jared G
authors.initials: JG
authors.lastname: Cobb
authors.forename: Jack
authors.initials: J
authors.lastname: Virostko
authors.forename: Jingping
authors.initials: J
authors.lastname: Xie
authors.forename: Amelie
authors.initials: A
authors.lastname: Gillman
authors.forename: Dmitry
authors.initials: D
authors.lastname: Koktysh
authors.forename: Denny
authors.initials: D
authors.lastname: Kerns
authors.forename: Mark
authors.initials: M
authors.lastname: Does
authors.forename: John C
authors.initials: JC
authors.lastname: Gore
authors.forename: Wellington
authors.initials: W
authors.lastname: Pham
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003287:Contrast Media / Q000138:chemical synthesis; D003713:Dendritic Cells / Q000166:cytology*; D005290:Ferric Compounds / Q000138:chemical synthesis*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide