title: Footprinting of protein interactions by tritium labeling.
abstract: A new footprinting method for mapping protein interactions has been developed, using tritium as a radioactive label. As residues involved in an interaction are less labeled when the complex is formed, they can be identified via comparison of the tritium incorporation of each residue of the bound protein with that of the unbound one. Application of this footprinting method to the complex formed by the histone H3 fragment H3(122-135) and the protein hAsf1A(1-156) afforded data in good agreement with NMR results.
authors.affiliation: IBITECS, CNRS CEA, F-91191 Gif-sur-Yvette, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Mousseau
authors.forename: Quentin
authors.initials: Q
authors.lastname: Raffy
authors.forename: Olivier P
authors.initials: OP
authors.lastname: Thomas
authors.forename: Morgane
authors.initials: M
authors.lastname: Agez
authors.forename: Robert
authors.initials: R
authors.lastname: Thai
authors.forename: Jean Philippe
authors.initials: JP
authors.lastname: Renault
authors.forename: Serge
authors.initials: S
authors.lastname: Pin
authors.forename: Françoise
authors.initials: F
authors.lastname: Ochsenbein
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Cintrat
authors.forename: Bernard
authors.initials: B
authors.lastname: Rousseau
mesh_terms: D000595:Amino Acid Sequence; D004526:Efficiency; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D008954:Models, Biological; D019906:Nuclear Magnetic Resonance, Biomolecular; D009707:Nucleosomes / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D023302:Protein Footprinting / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014316:Tritium
chemical_list: D006657:Histones; D009707:Nucleosomes; D010446:Peptide Fragments; D011506:Proteins; D014316:Tritium