title: Simultaneous determination of amino acids in discrete brain areas in Suncus murinus by high performance liquid chromatography with electrochemical detection.
abstract: An improved and simple reversed-phase high performance liquid chromatography method with electrochemical detection for the simultaneous determination of amino acids in brain tissue of Suncus murinus was developed. Homogenates from 5 different brain areas were derivatized with o-phthalaldehyde in the presence of sodium sulphite. Subsequent separation was achieved using linear gradient elution over 30 min. The derivatives were stable for up to 20 h at 4 degrees C. The method was accurate, reproducible, and showed good linearity. The recoveries were >88% for aspartate, glutamine, glutamate, glycine and gamma-aminobutyric acid, with the limit of quantification varying from 5 to 30 pmol. The method was successfully applied for the measurement of amino acids under fed and fasted conditions.
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China. stellacsw@cuhk.edu.hk
authors.forename: Sze Wa
authors.initials: SW
authors.lastname: Chan
authors.forename: Ge
authors.initials: G
authors.lastname: Lin
authors.forename: Kouichi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: David Tai Wai
authors.initials: DT
authors.lastname: Yew
authors.forename: John Anthony
authors.initials: JA
authors.lastname: Rudd
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D005260:Female; D057230:Limit of Detection; D012788:Shrews / Q000378:metabolism*
chemical_list: D000596:Amino Acids