Diversity of thermophilic fungi in Tengchong Rehai National Park revealed by ITS nucleotide sequence analyses.
The geothermal sites near neutral and alkalescent thermal springs in Tengchong Rehai National Park were examined through cultivation-dependent approach to determine the diversity of thermophilic fungi in these environments. Here, we collected soils samples in this area, plated on agar media conducive for fungal growth, obtained pure cultures, and then employed the method of internal transcribed spacer (ITS) sequencing combined with morphological analysis for identification of thermophilic fungi to the species level. In total, 102 strains were isolated and identified as Rhizomucor miehei, Chaetomium sp., Talaromyces thermophilus, Talaromyces byssochlamydoides, Thermoascus aurantiacus Miehe var. levisporus, Thermomyces lanuginosus, Scytalidium thermophilum, Malbranchea flava, Myceliophthora sp. 1, Myceliophthora sp. 2, Myceliophthora sp. 3, and Coprinopsis sp. Two species, T. lanuginosus and S. thermophilum were the dominant species, representing 34.78% and 28.26% of the sample, respectively. Our results indicated a greater diversity of thermophilic fungi in neutral and alkaline geothermal sites than acidic sites around hot springs reported in previous studies. Most of our strains thrived at alkaline growth conditions.
Pan
Wen-Zheng
WZ
Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, PR China.
Huang
Xiao-Wei
XW
Wei
Kang-Bi
KB
Zhang
Chun-Mei
CM
Yang
Dong-Mei
DM
Ding
Jun-Mei
JM
Zhang
Ke-Qin
KQ
D044822:Biodiversity; D002681:China; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D045482:Hot Springs / Q000382:microbiology*; D054892:Metagenome; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer