title: Insight into the Sialome of the Bed Bug, Cimex lectularius.
abstract: The evolution of insects to a blood diet leads to the development of a saliva that antagonizes their hosts' hemostasis and inflammation. Hemostasis and inflammation are redundant processes, and thus a complex salivary potion composed of dozens or near 100 different polypeptides is commonly found by transcriptome or proteome analysis of these organisms. Several insect orders or families evolved independently to hematophagy, creating unique salivary potions in the form of novel pharmacological use of endogenous substances and in the form of unique proteins not matching other known proteins, these probably arriving by fast evolution of salivary proteins as they evade their hosts' immune response. In this work we present a preliminary description of the sialome (from the Greek Sialo = saliva) of the common bed bug Cimex lectularius, the first such work from a member of the Cimicidae family. This manuscript is a guide for the supplemental database files http://exon.niaid.nih.gov/transcriptome/C_lectularius/S1/Cimex-S1.zip and http://exon.niaid.nih.gov/transcriptome/C_lectularius/S2/Cimex-S2.xls.
authors.affiliation: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Rockville, Maryland 20852, USA.
authors.forename: Ivo M B
authors.initials: IM
authors.lastname: Francischetti
authors.forename: Eric
authors.initials: E
authors.lastname: Calvo
authors.forename: John F
authors.initials: JF
authors.lastname: Andersen
authors.forename: Van M
authors.initials: VM
authors.lastname: Pham
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Favreau
authors.forename: Kent D
authors.initials: KD
authors.lastname: Barbian
authors.forename: Alvaro
authors.initials: A
authors.lastname: Romero
authors.forename: Jesus G
authors.initials: JG
authors.lastname: Valenzuela
authors.forename: José M C
authors.initials: JM
authors.lastname: Ribeiro
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001511:Bedbugs / Q000235:genetics* / Q000502:physiology; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D004798:Enzymes / Q000235:genetics; D005247:Feeding Behavior / Q000502:physiology*; D005260:Female; D015723:Gene Library; D019476:Insect Proteins / Q000302:isolation & purification*; D008297:Male; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D040901:Proteomics / Q000379:methods*; D012471:Salivary Proteins and Peptides / Q000302:isolation & purification*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D053719:Tandem Mass Spectrometry
chemical_list: D017931:DNA Primers; D004798:Enzymes; D019476:Insect Proteins; D012471:Salivary Proteins and Peptides; D000110:Acetylcholinesterase