title: Effect of manganese on the secretion of manganese-peroxidase by the basidiomycete Ceriporiopsis subvermispora.
abstract: The ligninolytic machinery of the widely used model fungus Ceriporiopsis subvermispora includes the enzymes manganese-peroxidase (MnP) and laccase (Lcs). In this work the effect of Mn(II) on the secretion of MnP was studied. Cultures grown in the absence of Mn(II) showed high levels of mnp transcripts. However, almost no MnP enzyme was detected in the extracellular medium, either by enzymatic activity assays or Western blot hybridizations. In the corresponding mycelia, immuno-electron microscopy experiments showed high levels of MnP enzyme within intracellular compartments. These results suggest that in addition to its well-known effect on transcription regulation of mnp genes, manganese influences secretion of MnP to the extracellular medium. Experiments carried out in the presence of cycloheximide confirmed that the metal is required to secrete MnP already synthesized and retained within the cell.
authors.affiliation: Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Mancilla
authors.forename: Paulo
authors.initials: P
authors.lastname: Canessa
authors.forename: Augusto
authors.initials: A
authors.lastname: Manubens
authors.forename: Rafael
authors.initials: R
authors.lastname: Vicuña
mesh_terms: D055453:Coriolaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D005786:Gene Expression Regulation; D042845:Laccase / Q000235:genetics / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology*; D016253:Microscopy, Immunoelectron; D025282:Mycelium / Q000235:genetics / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*
chemical_list: D008345:Manganese; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase