title: Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells.
abstract: In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role.
authors.affiliation: Laboratório de Fisiologia de Insetos Hematófagos, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: Fernanda F
authors.initials: FF
authors.lastname: Rocha
authors.forename: Ricardo N
authors.initials: RN
authors.lastname: Araujo
authors.forename: Luciana M
authors.initials: LM
authors.lastname: Silva
authors.forename: Nelder F
authors.initials: NF
authors.lastname: Gontijo
authors.forename: Marcos H
authors.initials: MH
authors.lastname: Pereira
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D012239:Rhodnius / Q000166:cytology*; D012469:Salivary Glands / Q000166:cytology* / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000378:metabolism
chemical_list: D012471:Salivary Proteins and Peptides