Platelet-rich fibrin membranes as scaffolds for periosteal tissue engineering.
OBJECTIVE
Platelet-rich fibrin (PRF)-based membranes have been used for covering alveolar ridge augmentation side in several in vivo studies. Few in vitro studies on PRF and no studies using human periosteal cells for tissue engineering have been published. The aim is a comparison of PRF with the commonly used collagen membrane Bio-Gide as scaffolds for periosteal tissue engineering.


METHODS
Human periosteal cells were seeded on membrane pieces (collagen [Bio-Gide] and PRF) at a density of 10(4) cells/well. Cell vitality was assessed by fluorescein diacetate (FDA) and propidium iodide (PI) staining, biocompatibility with the lactate dehydrogenase (LDH) test and proliferation level with the MTT, WST and BrdU tests and scanning electron microscopy (SEM).


RESULTS
PRF membranes showed slightly inferior biocompatibility, as shown by the LDH test. The metabolic activity measured by the MTT and WST tests was higher for PRF than for collagen (BioGide). The proliferation level as measured by the BrdU test (quantitative) and SEM examinations (qualitative) revealed higher values for PRF.


CONCLUSIONS
PRF appears to be superior to collagen (Bio-Gide) as a scaffold for human periosteal cell proliferation. PRF membranes are suitable for in vitro cultivation of periosteal cells for bone tissue engineering.
Gassling
Volker
V
Department of Oral and Maxillofacial Surgery, University of Schleswig-Holstein, Kiel, Germany. gassling@mkg.uni-kiel.de
Douglas
Timothy
T
Warnke
Patrick H
PH
Açil
Yahya
Y
Wiltfang
Jörg
J
Becker
Stephan T
ST
D000328:Adult; D000540:Alveolar Ridge Augmentation / Q000379:methods*; D001672:Biocompatible Materials; D001792:Blood Platelets; D001973:Bromodeoxyuridine; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen; D003584:Cytological Techniques / Q000379:methods; D005337:Fibrin / Q000494:pharmacology*; D005452:Fluoresceins; D006801:Humans; D007770:L-Lactate Dehydrogenase; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010521:Periosteum / Q000166:cytology*; D011419:Propidium; D013194:Staining and Labeling; D013778:Tetrazolium Salts; D013844:Thiazoles; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
C106416:Bio-Gide; D001672:Biocompatible Materials; D005452:Fluoresceins; D008567:Membranes, Artificial; D013778:Tetrazolium Salts; D013844:Thiazoles; D011419:Propidium; D005337:Fibrin; D003094:Collagen; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue; D001973:Bromodeoxyuridine; C018506:diacetylfluorescein