Reduction of PrP(C) in human cerebrospinal fluid after spinal cord injury.
It has been estimated that cerebrospinal fluid (CSF) contains approximately 80 proteins that significantly increase or decrease in response to various clinical conditions. Here we have evaluated the CSF protein PrP(C) (cellular prion protein) for possible increases or decreases following spinal cord injury. The physiological function of PrP(C) is not yet completely understood; however, recent findings suggest that PrP(C) may have neuroprotective properties. Our results show that CSF PrP(C) is decreased in spinal cord injured patients 12 h following injury and is absent at 7 days. Given that normal PrP(C) has been proposed to be neuroprotective we speculate that the decrease in CSF PrP(C) levels may influence neuronal cell survival following spinal cord injury.
Carnini
Anna
A
Hotchkiss Brain Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AL, CA.
Casha
Steve
S
Yong
V Wee
VW
Hurlbert
R John
RJ
Braun
Janice E A
JE
D048948:14-3-3 Proteins / Q000134:cerebrospinal fluid; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002408:Catheters, Indwelling; D015170:Cerebrospinal Fluid Pressure; D003459:Crystallins / Q000134:cerebrospinal fluid; D005260:Female; D006360:Heat-Shock Proteins / Q000134:cerebrospinal fluid; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D008297:Male; D008875:Middle Aged; D008911:Minocycline / Q000627:therapeutic use*; D018832:Molecular Chaperones / Q000134:cerebrospinal fluid; D010477:Perfusion; D018621:PrPC Proteins / Q000134:cerebrospinal fluid*; D013119:Spinal Cord Injuries / Q000134:cerebrospinal fluid* / Q000188:drug therapy* / Q000378:metabolism; D055815:Young Adult
D048948:14-3-3 Proteins; D003459:Crystallins; D006360:Heat-Shock Proteins; D007074:Immunoglobulin G; D018832:Molecular Chaperones; D018621:PrPC Proteins; D008911:Minocycline