Magnetic nanoparticles for imaging dendritic cells.
We report the development of superparamagnetic iron oxide (SPIOs) nanoparticles and investigate the migration of SPIO-labeled dendritic cells (DCs) in a syngeneic mouse model using magnetic resonance (MR) imaging. The size of the dextran-coated SPIO is roughly 30 nm, and the DCs are capable of independent uptake of these particles, although not at levels comparable to particle uptake in the presence of a transfecting reagent. On average, with the assistance of polylysine, the particles were efficiently delivered inside DCs within one hour of incubation. The SPIO particles occupy approximately 0.35% of cell surface and are equivalent to 34.6 pg of iron per cell. In vivo imaging demonstrated that the labeled cells migrated from the injection site in the footpad to the corresponding popliteal lymph node. The homing of labeled cells in the lymph nodes resulted in a signal drop of up to 79%. Furthermore, labeling DCs with SPIO particles did not compromise cell function, we demonstrated that SPIO-enhanced MR imaging can be used to track the migration of DCs effectively in vivo.
Kobukai
Saho
S
Vanderbilt University, Institute of Imaging Science, Nashville, Tennessee 37232-2310, USA.
Baheza
Richard
R
Cobb
Jared G
JG
Virostko
Jack
J
Xie
Jingping
J
Gillman
Amelie
A
Koktysh
Dmitry
D
Kerns
Denny
D
Does
Mark
M
Gore
John C
JC
Pham
Wellington
W
D000818:Animals; D002478:Cells, Cultured; D003287:Contrast Media / Q000138:chemical synthesis; D003713:Dendritic Cells / Q000166:cytology*; D005290:Ferric Compounds / Q000138:chemical synthesis*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide