Molecular cytogenetic characterization of stem-like cancer cells isolated from established cell lines.
There is indication that tumor growth is sustained by subpopulation of cells with stem-like features but little is known on their genomic characterization and their genetic stability. We report a detailed molecular cytogenetic characterization using Spectral Karyotyping and fluorescent in situ hybridization of parental serum-cultured adherent cells and their sphere-growing stem-like counterpart before and after differentiation from six cell lines established from solid tumors. Our findings indicate increased cytogenetic complexity in sphere-growing stem-like and their differentiated adherent cells compared to parental adherent component suggesting the existence within cell lines of heterogeneous and genetically unstable subpopulations of cells endowed with stem-like features.
Gasparini
Patrizia
P
Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
Bertolini
Giulia
G
Binda
Mara
M
Magnifico
Alessandra
A
Albano
Luisa
L
Tortoreto
Monica
M
Pratesi
Graziella
G
Facchinetti
Federica
F
Abolafio
Gabriella
G
Roz
Luca
L
Tagliabue
Elda
E
Daidone
Maria Grazia
MG
Sozzi
Gabriella
G
D015703:Antigens, CD / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002455:Cell Division; D045744:Cell Line, Tumor / Q000473:pathology*; D002869:Chromosome Aberrations; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping / Q000379:methods; D009369:Neoplasms / Q000235:genetics; D013234:Stem Cells / Q000473:pathology*
D015703:Antigens, CD; D014408:Biomarkers, Tumor