title: Degradation of nicotine in tobacco waste extract by newly isolated Pseudomonas sp. ZUTSKD.
abstract: The newly isolated Pseudomonas sp. ZUTSKD was evaluated for its ability to degrade nicotine in tobacco waste extract (TWE). The strain degraded nicotine completely when the concentration of reducing sugar in TWE was lower than 8 gL(-1). Yeast extract and phosphate additions improved nicotine degradation in 5% TWE. At 30 degrees C and pH 7.0, with additional 15 gL(-1) Na(2)HPO(4).6H(2)O and 6 gL(-1) KH(2)PO(4) in 5% TWE, Pseudomonas sp. ZUTSKD could degrade 97% of nicotine (1.6 gL(-1)) in 12h. The data showed that strain ZUTSKD could be useful to control the nicotine content in TWE.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, PR China. whzhong@zjut.edu.cn
authors.forename: Weihong
authors.initials: W
authors.lastname: Zhong
authors.forename: Chenjing
authors.initials: C
authors.lastname: Zhu
authors.forename: Ming
authors.initials: M
authors.lastname: Shu
authors.forename: Kedan
authors.initials: K
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Chang
authors.initials: C
authors.lastname: Wang
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Ye
authors.forename: Jianmeng
authors.initials: J
authors.lastname: Chen
mesh_terms: D001673:Biodegradation, Environmental; D007220:Industrial Waste / Q000517:prevention & control*; D009538:Nicotine / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D013045:Species Specificity; D014026:Nicotiana / Q000382:microbiology*
chemical_list: D007220:Industrial Waste; D009538:Nicotine