Species-difference of cyclooxygenase-2 in the hippocampus of rodents.
Cyclooxygenase (COX) generates free radicals and it is important in inflammatory response. In this study, we observed the immunoreactivity in mice (ICR and C57BL/6 strain), rats and gerbils. In these animals, COX-2 immunoreactivity was mainly detected in pyramidal cells of the hippocampal CA2/3 region and in granule cells of the dentate gyrus. COX-2 immunoreactivity in the CA2/3 region was the highest in ICR mice, while in gerbils COX-2 immunoreactivity was the lowest; COX-2 immunoreactivity in the dentate gyrus was the highest in rats and the lowest in gerbils. The protein levels of COX-2 were similar to the immunohistochemical data. COX-2 mRNA transcript was the highest in the gerbil and the lowest in the rat. In brief, COX-2 protein, not mRNA, in the hippocampus is generally higher in mice (ICR and C57BL/6 strain) than rats and gerbils.
Kim
Sung Koo
SK
Department of Pediatrics, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, South Korea.
Choi
Ji Won
JW
Hwang
In Koo
IK
Yoo
Dae Young
DY
Yoo
Ki-Yeon
KY
Lee
Choong Hyun
CH
Choi
Jung Hoon
JH
Shin
Hyung-Cheul
HC
Seong
Je Kyung
JK
Yoon
Yeo Sung
YS
Won
Moo-Ho
MH
D000818:Animals; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D005849:Gerbillinae; D006624:Hippocampus / Q000201:enzymology*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D019579:Neocortex / Q000201:enzymology; D009474:Neurons / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity
D017931:DNA Primers; D051546:Cyclooxygenase 2