[Localization of polypeptides release factors and ribosome protein L11 in Euplotes octocarinatus].
Protozoan ciliates are a group of unicellular eukaryotes. The special characteristics of stop codons usage in termination of protein biosynthesis in ciliates cells makes them an ideal model to study the mechanism of stop codon recognition of polypeptides release factors. To localize the functional positions of biomolecules in ciliates cell, we constructed a macronuclear artificial chromosome containing a gene encoding red fluorescence protein (EoMAC_R) based on the structural characteristics of ciliates chromosome. Three factors, L11, eRF1a, and eRF3 that are involved in termination process of protein synthesis were colocalized in Euplotes octocarinatus cells by using novel EoMAC_R and the previously constructed EoMAC_G. The results indicated that protein synthesis mainly occurred inside the "C" shape macronucleus, suggesting that EoMAC could be a useful tool for localizing biomolecules in ciliates cell.
Chai
Baofeng
B
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, China. bfchai@sxu.edu.cn
Li
Na
N
Wang
Jingtao
J
Shen
Quan
Q
Zhang
Zhiyun
Z
Liang
Aihua
A
D022201:Chromosomes, Artificial; D018388:Codon, Terminator / Q000378:metabolism; D016812:Euplotes / Q000737:chemistry*; D010454:Peptide Termination Factors / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000235:genetics; D015800:Protozoan Proteins / Q000032:analysis / Q000235:genetics; D012269:Ribosomal Proteins / Q000032:analysis* / Q000235:genetics
D018388:Codon, Terminator; D010454:Peptide Termination Factors; D010455:Peptides; D015800:Protozoan Proteins; D012269:Ribosomal Proteins; C027624:ribosomal protein L11