Chronic sleep deprivation markedly reduces coagulation factor VII expression.
Chronic sleep loss, a common feature of human life in industrialized countries, is associated to cardiovascular disorders. Variations in functional parameters of coagulation might contribute to explain this relationship. By exploiting the mouse model and a specifically designed protocol, we demonstrated that seven days of partial sleep deprivation significantly decreases (-30.5%) the thrombin generation potential in plasma evaluated upon extrinsic (TF/FVIIa pathway) but not intrinsic activation of coagulation. This variation was consistent with a decrease (-49.8%) in the plasma activity levels of factor VII (FVII), the crucial physiologicalal trigger of coagulation, which was even more pronounced at the liver mRNA level (-85.7%). The recovery in normal sleep conditions for three days completely restored thrombin generation and FVII activity in plasma. For the first time, we demonstrate that chronic sleep deprivation on its own reduces, in a reversible manner, the FVII expression levels, thus influencing the TF/FVIIa activation pathway efficiency.
Pinotti
Mirko
M
Neuroscience Institute, Morehouse School of Medicine, 720 Westview Dr, Atlanta, GA 30310, USA.
Bertolucci
Cristiano
C
Frigato
Elena
E
Branchini
Alessio
A
Cavallari
Nicola
N
Baba
Kenkichi
K
Contreras-Alcantara
Susana
S
Ehlen
J Christopher
JC
Bernardi
Francesco
F
Paul
Ketema N
KN
Tosini
Gianluca
G
D000818:Animals; D002908:Chronic Disease; D005167:Factor VII / Q000235:genetics* / Q000378:metabolism; D015942:Factor VIIa / Q000378:metabolism; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D012892:Sleep Deprivation / Q000097:blood* / Q000503:physiopathology*; D013917:Thrombin / Q000378:metabolism; D013925:Thromboplastin / Q000378:metabolism; D013997:Time Factors; D015431:Weight Loss / Q000502:physiology
D005167:Factor VII; D013925:Thromboplastin; D015942:Factor VIIa; D013917:Thrombin