Cramoll 1,4 lectin increases ROS production, calcium levels, and cytokine expression in treated spleen cells of rats.
This study reports the in vivo stimulatory effects of Cramoll 1,4 on rat spleen lymphocytes as evidenced by an increase in intracellular reactive oxygen species (ROS) production, Ca(2+) levels, and interleukin (IL)-1beta expression. Cramoll 1,4 extracted from seeds of the Leguminosae Cratylia mollis Mart., is a lectin with antitumor and lymphocyte mitogenic activities. Animals (Nine-week-old male albino Wistar rats, Rattus norvegicus) were treated with intraperitoneal injection of Cramoll 1,4 (235 microg ml(-1) single dose) and, 7 days later, spleen lymphocytes were isolated and analyzed for intracellular ROS, cytosolic Ca(2+), and IL-6, IL-10, and IL-1 mRNAs. Cell viability was investigated by annexin V-FITC and 7-amino-actinomycin D staining. The data showed that in lymphocytes activated by Cramoll 1,4 the increase in cytosolic and mitochondrial ROS was related to higher cytosolic Ca(2+) levels. Apoptosis and necrosis were not detected in statistically significant values and thus the lectin effector activities did not induce lymphocyte death. In vivo Cramoll 1,4 treatment led to a significant increase in IL-1beta but IL-6 and -10 levels did not change. Cramoll 1,4 had modulator activities on spleen lymphocytes and stimulated the Th2 response.
de Melo
Cristiane Moutinho Lagos
CM
Departamento de Imunologia, Instituto de Pesquisas Aggeu Magalhães (IAM/FIOCRUZ), Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil. crismout_melo@hotmail.com
Paim
Bruno Alves
BA
Zecchin
Karina Gotardelo
KG
Morari
Joseani
J
Chiaratti
Marcos R
MR
Correia
Maria Tereza Santos
MT
Barroso Coelho
Luana Cassandra B
LC
Paiva
Patrícia Maria Guedes
PM
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D007274:Injections, Intraperitoneal; D016753:Interleukin-10 / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D037121:Plant Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D053583:Interleukin-1beta; D015850:Interleukin-6; D037121:Plant Lectins; D017382:Reactive Oxygen Species; D016753:Interleukin-10; D002118:Calcium