Chemoenzymatic synthesis of the sialyl Lewis X glycan and its derivatives.
A combination of recombinant FKP and alpha-(1-->3)-fucosyltransferase allows the facile synthesis of the sialyl Lewis X tetrasaccharide glycan and its derivatives in excellent yield. In this system, the universal fucosyl donor, guanidine 5'-diphosphate-beta-L-fucose (GDP-fucose), or its analogues can be generated in situ by cofactor recycling using pyruvate kinase.
Soriano del Amo
David
D
Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461, USA.
Wang
Wei
W
Besanceney
Christen
C
Zheng
Tianqing
T
He
Yizheng
Y
Gerwe
Brian
B
Seidel
Ronald D
RD
Wu
Peng
P
D000606:Amino Sugars / Q000737:chemistry / Q000378:metabolism; D002240:Carbohydrate Sequence; D005643:Fucose / Q000737:chemistry / Q000378:metabolism; D005647:Fucosyltransferases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000378:metabolism*; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D000080506:Sialyl Lewis X Antigen; D013237:Stereoisomerism; D013379:Substrate Specificity
D000606:Amino Sugars; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; C032233:lactosamine; D005643:Fucose; D005647:Fucosyltransferases; C115708:GDP-L-fucose pyrophosphorylase; D009713:Nucleotidyltransferases