Effects of lifestyle modification on oxidized LDL, reactive oxygen species production and endothelial cell viability in patients with coronary artery disease.
OBJECTIVE
We evaluated the effects of lifestyle modification (LM) on lipid profile, oxidative stress and serum-stimulated human coronary artery endothelial cell (HCAEC) viability in coronary artery disease (CAD) patients after 6months.


METHODS
Thirty patients with CAD were randomly assigned to LM intervention (n=15) and usual care control (n=15) groups. LM-intervened patients were instructed to consume low-fat, high-antioxidants and fiber diets. Moderate exercise and stress management were also advised. Group support to maintain patients' compliance was applied.


RESULTS
Serum cholesterol, triglyceride, oxidized LDL and protein carbonyl were decreased in LM group. Serum triglyceride was increased in control group. HCAEC viability was increased, while intracellular reactive oxygen species was decreased, by serum from the LM group.


CONCLUSIONS
LM is capable of improving lipid profile, reducing oxidative stress and increasing HCAEC survival in the patients with CAD, hence lowering a risk for the future cardiovascular event.
Srimahachota
Suphot
S
Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10130, Thailand.
Wunsuwan
Rattiporn
R
Siritantikorn
Atchasai
A
Boonla
Chanchai
C
Chaiwongkarjohn
Suttirak
S
Tosukhowong
Piyaratana
P
D000368:Aged; D000975:Antioxidants / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood / Q000378:metabolism*; D004032:Diet; D004043:Dietary Fiber / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D008019:Life Style; D008077:Lipoproteins, LDL / Q000097:blood* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D017382:Reactive Oxygen Species / Q000097:blood / Q000378:metabolism*; D012307:Risk Factors; D013315:Stress, Psychological / Q000628:therapy; D015996:Survival Rate; D014280:Triglycerides / Q000097:blood
D000975:Antioxidants; D004043:Dietary Fiber; D008077:Lipoproteins, LDL; D017382:Reactive Oxygen Species; D014280:Triglycerides; C099252:oxidized low density lipoprotein; D002784:Cholesterol