[Kallikrein-binding protein promotes axonal regeneration and protect rat retinal ganglion cells following optic nerve injury].
OBJECTIVE
To explore the effect of kallikrein-binding protein (KPB) in protecting retinal ganglion cells (RGCs) and promoting axonal regeneration following optical nerve injury in rats.


METHODS
Crush injury of the optic nerve at 0.5-1.0 mm from the eyeball was induced in rats, which received subsequent KBP injection into the vitreous cavity (experimental group) and PBS injection (control group). At 7, 14 and 21 days after the injury, the rats were sacrificed and frozen sections of the eyeball were prepared to observe the structure and thickness of the retina and count the number of survival RGCs with HE staining. The optic nerves were collected for Western blotting to assess the effect of KBP on the RGCs and axonal regeneration.


RESULTS
RGC counts and retinal thickness showed significant differences between the two groups. Western blotting also demonstrated a significant difference in the expression of the nerve regeneration marker protein GAP-43 between the two groups.


CONCLUSIONS
KBP offers protection on RGCs and promotes regeneration of the optic nerve axons after optic nerve injury in rats.
Jin
Shun-xiang
SX
Department of Ophthalmology, Kunming Children's Hospital, Kunming Medical College, Kunming 650031, China.
Zeng
Yong
Y
Wan
Jing
J
Wan
Kun
K
Li
Yuan-yuan
YY
Li
Li-yan
LY
Wang
Ting-hua
TH
Feng
Zhong-tang
ZT
Li
Yan
Y
D000818:Animals; D001369:Axons / Q000502:physiology; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020221:Optic Nerve Injuries / Q000188:drug therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000502:physiology; D015843:Serpins / Q000494:pharmacology*
D019922:GAP-43 Protein; D018696:Neuroprotective Agents; D015843:Serpins; C051324:kallistatin