title: Ovine PrP genotype is linked with lesion profile and immunohistochemistry patterns after primary transmission of classical scrapie to wild-type mice.
abstract: It is currently believed that primary transmission of classical scrapie to wild-type mice is inefficient and characterized by low attack rates and variable incubation periods and lesion profiles. Consequently, strain characterization of classical scrapie in these mice relies on subpassage. The aim of this study was to perform a retrospective analysis of lesion profiles and immunohistochemistry patterns after transmission of a large number of classical scrapie sources to wild-type mice and to investigate trends that might be used to characterize the agent without subpassaging. Scrapie field cases (n = 31) collected from individual farms between 1996 and 1999 were inoculated into RIII, C57BL, and VM mice and profiled using standard methodology and analyzed by immunohistochemistry. Using cluster analysis to resultant lesion profiles produced groups of similar lesion profiles in RIII and C57BL mice. We observed correlations between lesion profile clusters and the ovine prion protein (PrP) genotype. Immunohistochemistry indicated donor-mediated trends in the PrP pattern. These results indicate that ovine PrP genotype is a factor that is linked to both the lesion profile and the pattern of PrP deposition on primary transmission of classical scrapie to wild-type mice.
authors.affiliation: Neuropathology Unit, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey, United Kingdom.
authors.forename: Katy E
authors.initials: KE
authors.lastname: Beck
authors.forename: Rosemary E
authors.initials: RE
authors.lastname: Sallis
authors.forename: Richard
authors.initials: R
authors.lastname: Lockey
authors.forename: Marion M
authors.initials: MM
authors.lastname: Simmons
authors.forename: John
authors.initials: J
authors.lastname: Spiropoulos
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology*; D002452:Cell Count / Q000379:methods; D016000:Cluster Analysis; D004195:Disease Models, Animal; D005838:Genotype; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D018620:PrPSc Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D017452:Receptors, IgG / Q000235:genetics; D012189:Retrospective Studies; D012608:Scrapie / Q000235:genetics / Q000473:pathology / Q000635:transmission; D012756:Sheep
chemical_list: D009419:Nerve Tissue Proteins; D018620:PrPSc Proteins; D017452:Receptors, IgG