Synthesis of cyclopropane fatty acids in Lactobacillus helveticus and Lactobacillus sanfranciscensis and their cellular fatty acids changes following short term acid and cold stresses.
An implemented GC method to separate and quantify the cell cyclopropane fatty acids lactobacillic (C19cyc11) and dehydrosterculic (C19cyc9) was used to study the adaptive response to sublethal acid and cold stresses in Lactobacillus helveticus and Lactobacillus sanfranciscensis. The comparison of the composition of cellular fatty acids of the two strains and their changes after 2 h of stress exposure under micro-aerobic and anaerobic conditions indicated that the aerobic biosynthetic pathway for unsaturated fatty acids is prevalent in L. sanfranciscensis, while the anaerobic pathway is prevalent in L. helveticus. Indeed in the latter strain, in the presence of a source of oleic acid and under micro-aerobic conditions, C18:1n11 and its post-synthetic derivative C19cyc11 accounted for overall proportion ranging from 52 to 28% of the total FAs. On the other hand L. sanfranciscensis synthesizes by aerobic pathway C18:1n9 and transforms it to C19cyc9. However in this species the cumulative level of these two FAs did not exceed 30%. The relevant proportion of dodecanoic acid in the latter species suggests that carbon chain shortening is the principal strategy of L. sanfranciscensis to modulate fluidity or chemico-physical properties of the membranes.
Montanari
Chiara
C
Dipartimento di Scienze degli Alimenti, Alma Mater Studiorum, Università degli Studi di Bologna, Viale Fanin, 46, 40127 Bologna, Italy.
Sado Kamdem
Sylvain L
SL
Serrazanetti
Diana I
DI
Etoa
François-Xavier
FX
Guerzoni
M Elisabetta
ME
D000222:Adaptation, Physiological; D002849:Chromatography, Gas; D003080:Cold Temperature; D003521:Cyclopropanes / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis* / Q000096:biosynthesis*; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000378:metabolism / Q000502:physiology*; D052198:Lactobacillus helveticus / Q000378:metabolism / Q000502:physiology*; D008563:Membrane Lipids / Q000032:analysis; D050296:Microbial Viability; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*
D003521:Cyclopropanes; D005227:Fatty Acids; D008563:Membrane Lipids; C028775:cyclopropane fatty acids; D010100:Oxygen