Suppression of nitric oxide implicated in the protective effect of echinacoside on H2O2-induced PC12 cell injury.
We investigated whether suppression of nitric oxide (NO) implicated in the protective effect of echinacoside (ECH), a phenylethanoid glycoside, on H2O2-induced injury to the rat pheochromocytoma cell line (PC12 cells). Data show that application of ECH to H2O2-injured PC12 cells (HIPCs) increased cell viability and decreased the necrotic ratio. Laser scanning confocal microscopic (LSCM) analysis suggested that ECH exerted an inhibitory effect on the formation of NO. In addition, RT-PCR analysis revealed that ECH down-regulated p65 and iNOS mRNA expressions in HIPCs. In summary, suppression of NO is related to the protective effect of ECH on HIPCs.
Kuang
Rong
R
Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering, Ministry of Education, Zhejiang University, Hang Zhou, PR China.
Sun
Yiguo
Y
Zheng
Xiaoxiang
X
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D006027:Glycosides / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018613:Microscopy, Confocal; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D016716:PC12 Cells; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051996:Transcription Factor RelA / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D006027:Glycosides; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D052247:Nitric Oxide Synthase Type II; C060297:echinacoside