Genome survey sequence analysis and identification of homologs of major surface protease (gp63) genes in Trypanosoma rangeli.
In this study, 222 genome survey sequences were generated for Trypanosoma rangeli strain P07 isolated from an opossum (Didelphis albiventris) in Minas Gerais State, Brazil. T. rangeli sequences were compared by BLASTX (Basic Local Alignment Search Tool X) analysis with the assembled contigs of Leishmania braziliensis, Leishmania infantum, Leishmania major, Trypanosoma brucei, and Trypanosoma cruzi. Results revealed that 82% (182/222) of the sequences were associated with predicted proteins described, whereas 18% (40/222) of the sequences did not show significant identity with sequences deposited in databases, suggesting that they may represent T. rangeli-specific sequences. Among the 182 predicted sequences, 179 (80.6%) had the highest similarity with T. cruzi, 2 (0.9%) with T. brucei, and 1 (0.5%) with L. braziliensis. Computer analysis permitted the identification of members of various gene families described for trypanosomatids in the genome of T. rangeli, such as trans-sialidases, mucin-associated surface proteins, and major surface proteases (MSP or gp63). This is the first report identifying sequences of the MSP family in T. rangeli. Multiple sequence alignments showed that the predicted MSP of T. rangeli presented the typical characteristics of metalloproteases, such as the presence of the HEXXH motif, which corresponds to a region previously associated with the catalytic site of the enzyme, and various cysteine and proline residues, which are conserved among MSPs of different trypanosomatid species. Reverse transcriptase-polymerase chain reaction analysis revealed the presence of MSP transcripts in epimastigote forms of T. rangeli.
Ferreira
Keila A M
KA
Departamento de Ciências Biológicas, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.
Ruiz
Jeronimo C
JC
Dias
Fabrício C
FC
Lages-Silva
Eliane
E
Tosi
Luiz R O
LR
Ramírez
Luis E
LE
Pedrosa
André L
AL
D000595:Amino Acid Sequence; D030541:Databases, Genetic; D015971:Gene Expression Regulation, Enzymologic; D018503:Genome, Protozoan / Q000235:genetics*; D023281:Genomics; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017421:Sequence Analysis; D057509:Trypanosoma rangeli / Q000201:enzymology* / Q000235:genetics*
D010447:Peptide Hydrolases