title: [Preparation and characterization of specific monoclonal antibodies against HER2].
abstract: OBJECTIVE
  To prepare and characterize the specific monoclonal antibodies (mAbs) that can recognize the native conformation of HER2 so as to guide the clinical application of Herceptin.
  
  
  METHODS
  Hybridomas were generated by the fusion with the NS1 myeloma cell and spleen cell, which were from mice immunized by HER2 recombinant protein. After cell fusion, ELISA, Yeast cell base ELISA and immunohistochemistry were used to screening clones respectively.
  
  
  RESULTS
  Eight clones that could react with SK-Br-3 and yeast displaying HER2 were selected from 168 clones which were positive in initial screening by ELISA.
  
  
  CONCLUSIONS
  Successfully prepared 8 mAbs that can recognize the native conformation of HER2, which should provide useful reagent for personalized therapy against breast cancer with Herceptin.
authors.affiliation: Union Stem Cell &Gene Engineering Co., Ltd., Tianjin 300384, China.
authors.forename: Hong-qin
authors.initials: HQ
authors.lastname: Liu
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Qu
authors.forename: Lin
authors.initials: L
authors.lastname: Shi
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Huang
authors.forename: Chun-ling
authors.initials: CL
authors.lastname: Feng
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Zhang
authors.forename: Wei-bin
authors.initials: WB
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology* / Q000627:therapeutic use; D061067:Antibodies, Monoclonal, Humanized; D000918:Antibody Specificity; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006825:Hybridomas; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011487:Protein Conformation; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry / Q000276:immunology*; D000068878:Trastuzumab
chemical_list: D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D018719:Erb-b2 Receptor Tyrosine Kinases; D000068878:Trastuzumab