Identification of protein-bound dinitrosyl iron complexes by nuclear resonance vibrational spectroscopy.
We have applied (57)Fe nuclear resonance vibrational spectroscopy (NRVS) to identify protein-bound dinitrosyl iron complexes. Intense NRVS peaks due to vibrations of the N-Fe-N unit can be observed between 500 and 700 cm(-1) and are diagnostic indicators of the type of iron dinitrosyl species present. NRVS spectra for four iron dinitrosyl model compounds are presented and used as benchmarks for the identification of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.
Tonzetich
Zachary J
ZJ
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Wang
Hongxin
H
Mitra
Devrani
D
Tinberg
Christine E
CE
Do
Loi H
LH
Jenney
Francis E
FE
Adams
Michael W W
MW
Cramer
Stephen P
SP
Lippard
Stephen J
SJ
D019843:Archaeal Proteins / Q000737:chemistry / Q000378:metabolism; D005288:Ferredoxins / Q000737:chemistry / Q000378:metabolism; D007501:Iron / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D020027:Pyrococcus furiosus; D013057:Spectrum Analysis; D014732:Vibration
D019843:Archaeal Proteins; D005288:Ferredoxins; D009589:Nitrogen Oxides; D011506:Proteins; C073661:dinitrosyl iron complex; D007501:Iron