AFM imaging and analysis of electrostatic double layer forces on single DNA molecules.
Electrical double layer (EDL) forces develop between charged surfaces immersed in an electrolyte solution. Biological material surrounded by its physiological medium constitutes a case where these forces play a major role. Specifically, this work is focused on the study of the EDL force exerted by DNA molecules, a standard reference for the study of single biomolecules of nanometer size. The molecules deposited on plane substrates have been characterized by means of the atomic force microscope operated in the force spectroscopy imaging mode. Force spectroscopy imaging provides images of the topography of the DNA molecules, and of the EDL force spectrum. Due to the size of the molecule being much smaller than that of the tip, both the tip-substrate and tip-molecule interactions need to be considered in the analysis of the experimental results. We solve this problem by linearly superposing the two contributions. EDL force images are presented where DNA molecules are clearly resolved. The lateral resolution of the EDL force is discussed and compared with that of the topography. The method also allows the estimation of the DNA surface charge density, thereby obtaining reasonable values.
Sotres
J
J
Instituto de Ciencia de Materiales de Madrid (Consejo Superior de Investigaciones Científicas), Madrid, Spain.
Baró
A M
AM
D010582:Bacteriophage lambda; D001696:Biomechanical Phenomena; D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D055672:Static Electricity
D004247:DNA