[The telomerase activity of human adipose derived stem cells during proliferation and differentiation in vitro].
OBJECTIVE
To investigate the telomerase activity of human adipose derived stem cells (ADSCs) during proliferation and differentiation in vitro.


METHODS
ADSCs were highly purified and cultured in vitro. The morphology, phenotype and biological properties of the cultured ADSCs were observed by flow cytometer. Then ADSCs were induced to differentiate into adipocytes and osteoblast. The telomerase activity was detected by TRAP.


RESULTS
ADSCs had the ability of multi-directed differentiation, like adipocytes and osteoblast. It could also express the stem cell-related surface markers. The telomerase activity was negative or lowly expressed in ADSCs in vitro within 12 generations. The telomerase activity was up-regulated when ADSCs was adipogenic differentiated, but deceased 3-6 days later.


CONCLUSIONS
The telomerase activity of ADSCs is not changed during culture in vitro. It is up-regulated when ADSCs are induced to adipogenic differentiation, but decreased later.
Chen
Guang-ping
GP
Department of Aestethic and Plastic Surgery, Guangdong No. 2 Provincial People's Hospital, Guangzhou 510317, China.
Luo
Sheng-kang
SK
Wang
Hai-bin
HB
Sun
Zhong-sheng
ZS
Xu
Xiang
X
D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D000328:Adult; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*; D055815:Young Adult
D019098:Telomerase