The mechanism of vitexin-4''-O-glucoside protecting ECV-304 cells against tertbutyl hydroperoxide induced injury.
The aim of this article is to investigate the mechanism of vitexin-4''-O-glucoside (VOG) protecting ECV-304 cells against tertbutyl hydroperoxide (TBHP)-induced injury. ECV-304 cell viability was measured by MTT assay. Apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay. Cellular morphological changes were observed using phase contrast microscopy. The change of relative mitochondrial transmembrane potential in the ECV-304 cells was analysed with rhodamine 123 staining. Lipid peroxidation was measured by the HPLC method. The results showed that 128 µmol L(-1) VOG could effectively protect ECV-304 cells against cytotoxicity induced by TBHP. VOG protected TBHP-treated ECV-304 cells from death, significantly decreased MDA production, and increased superoxide dismutase (SOD) activity and mitochondrial membrane potential (ΔΨ). Taken together, VOG protects against TBHP-induced ECV-304 cell injury partially through resuming mitochondrial function.
Li
Hai-bo
HB
School of Basic Medical Sciences, Liaoning University of TCM, Shenyang 110032, China. lnlhb@hotmail.com
Ying
Xi-xiang
XX
Lu
Jia
J
D000704:Analysis of Variance; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D027825:Crataegus / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D047309:Flavones / Q000494:pharmacology*; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008858:Microscopy, Phase-Contrast; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D020112:Rhodamine 123 / Q000493:pharmacokinetics; D013482:Superoxide Dismutase / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
D004365:Drugs, Chinese Herbal; D047309:Flavones; D005960:Glucosides; D007529:Isoflavones; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; C477953:isovitexin-4'-O-glucoside; D020112:Rhodamine 123; D020122:tert-Butylhydroperoxide; D013482:Superoxide Dismutase; C022616:thiazolyl blue