Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3.
The characterization of new cell lines is an important tool to understand the biological processes involved in cancer treatments. In the present study, we used two newly established epithelial human breast cancer cell lines from primary sites MACL-1 and MGSO-3 and compared their susceptibility to the treatment with ionizing radiation (IR) with the commercial cell line MDA-MB-231. In the doses used (10 or 20 Gy), IR induced a reduction in cell viability and cell death, measured as DNA fragmentation, at 48 and 72 h after treatment. In addition, 48 h after treatment with IR, we observed an enhancement in the percentage of apoptotic cells. The broad-range caspases inhibitor zVAD-FMK inhibited cytotoxicity induced by IR. After 24 h, treatment with IR activated caspase-9 in MACL-1 and MDA-MB-231 but not in MGSO-3 cells. Thirty hours after treatment with IR (20 Gy), we observed an activation of caspases 8 and 3. These results suggest the involvement of caspases in the cell death induced by IR in two newly established cell lines. These cells may be useful in studies of breast cancer in defining basic mechanisms in molecular and cellular radiobiology and may contribute to the rational design of future models of cancer therapies.
Bertollo
Caryne Margotto
CM
Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Caixa Postal 486, CEP 31.270-901, Belo Horizonte, Minas Gerais, Brazil.
Correa
Cristiane Rodrigues
CR
Gomes
Dawidson Assis
DA
Souza-Fagundes
Elaine Maria
EM
Goes
Alfredo Miranda
AM
D001943:Breast Neoplasms / Q000378:metabolism / Q000532:radiotherapy*; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D053938:DNA Fragmentation / Q000528:radiation effects; D005260:Female; D006801:Humans; D010718:Phosphatidylserines / Q000378:metabolism
D010718:Phosphatidylserines; D020169:Caspases