title: Development of a recombinant epsilon toxoid vaccine against enterotoxemia and its use as a combination vaccine with live attenuated sheep pox virus against enterotoxemia and sheep pox.
abstract: Sheep pox and enterotoxemia are important diseases of sheep, and these diseases cause severe economic losses to sheep farmers. The present study was undertaken to evaluate the potential of formaldehyde-inactivated recombinant epsilon toxin as a vaccine candidate. The potency of the recombinant epsilon toxoid with aluminum hydroxide as an adjuvant in sheep was determined. Vaccinated sheep were protected against enterotoxemia, with potency values of >5 IU being protective. Further, the use of this construct in a combination vaccine against sheep pox resulted in the sheep being protected against both sheep pox and enterotoxemia.
authors.affiliation: Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad 500032, India.
authors.forename: Dev
authors.initials: D
authors.lastname: Chandran
authors.forename: Sureddi Satyam
authors.initials: SS
authors.lastname: Naidu
authors.forename: Parthasarathy
authors.initials: P
authors.lastname: Sugumar
authors.forename: Gudavalli Sudha
authors.initials: GS
authors.lastname: Rani
authors.forename: Shahana Pallichera
authors.initials: SP
authors.lastname: Vijayan
authors.forename: Deepika
authors.initials: D
authors.lastname: Mathur
authors.forename: Lalit C
authors.initials: LC
authors.lastname: Garg
authors.forename: Villuppanoor Alwar
authors.initials: VA
authors.lastname: Srinivasan
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D018151:Capripoxvirus / Q000276:immunology*; D004767:Enterotoxemia / Q000276:immunology / Q000517:prevention & control*; D005557:Formaldehyde / Q000494:pharmacology; D011213:Poxviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control* / Q000821:virology; D014121:Toxoids / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000276:immunology; D017778:Vaccines, Combined; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
chemical_list: D001427:Bacterial Toxins; D001428:Bacterial Vaccines; C039811:Clostridium perfringens epsilon-toxin; D014121:Toxoids; D014613:Vaccines, Attenuated; D017778:Vaccines, Combined; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D005557:Formaldehyde