Toxicological effects of ultraviolet radiation on lymphocyte cells with different manganese superoxide dismutase Ala16Val polymorphism genotypes.
The aim of this study was to investigate whether there is a differential response of lymphocytes from healthy MnSOD genotype subjects to oxidative stress. We used UV radiation as a toxic agent due to its genotoxic effects associated with chromosome aberrations caused by breaks in the DNA strands. Cellular growth rate, cell viability, mitotic index, chromosomal instability and biomarkers of oxidative metabolism were analysed in lymphocyte cells from healthy adults with different Ala16Val MnSOD polymorphisms that produce tree genotypes: AA, VV and AV. We found a differential response to UV exposure in cultures of lymphocyte cells from Ala16Val genotype donors. In general, AA cell cultures presented higher viability and mitotic index and lower TBARS levels than VV and AV cells for both the control and UV exposure groups. However, when we compared the DNA damage among the three genotypes, AA lymphocyte cells presented the highest damage from UV exposure. These data suggest that the Ala16Val polymorphism affects the response of cellular oxidative metabolism in different ways.
dos Santos Montagner
Greice Franciele Feyh
GF
Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Av Roraima 1000, Prédio 18, 97105900 Santa Maria, RS, Brazil. greiceijui@ibest.com.br
Sagrillo
Michele
M
Machado
Michel Mansur
MM
Almeida
Renata Chequeller
RC
Mostardeiro
Clarice Pinheiro
CP
Duarte
Marta Maria Medeiros Frescura
MM
da Cruz
Ivana Beatrice Mânica
IB
D000328:Adult; D015415:Biomarkers / Q000097:blood; D002470:Cell Survival / Q000528:radiation effects; D043171:Chromosomal Instability / Q000528:radiation effects; D020552:Comet Assay; D004249:DNA Damage; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D008940:Mitotic Index; D018384:Oxidative Stress / Q000528:radiation effects*; D020641:Polymorphism, Single Nucleotide; D013482:Superoxide Dismutase / Q000235:genetics*; D014466:Ultraviolet Rays; D055815:Young Adult
D015415:Biomarkers; D013482:Superoxide Dismutase