Thermosensitive micelles from PEG-based ether-anhydride triblock copolymers.
OBJECTIVE
The thermosensitive micelles based on the poly(PEG:CPP:SA) terpolymer composed of poly(ethylene glycol) (PEG), 1,3-bis(carboxyphenoxy) propane (CPP) and sebacic acid (SA) were fabricated for application as a promising drug carrier.


METHODS
The terpolymer can self-assemble into micelles in water by a precipitation technology. The sol-gel transition behaviors were investigated by the tube-tilting method and dynamic rheology. The drug release behaviors were investigated in phosphate-buffered solution (PBS) at 25, 37 and 45 degrees C, respectively, and the tumor cell growth inhibition assays were also evaluated.


RESULTS
The diameters of these micelles increased as the environmental temperature, and the length of CPP and SA chains increased. The micelles with a low concentration underwent sol-to-nanogel transition as temperature increased from the room temperature to the body temperature, while the polymer solutions with a high concentration underwent sol-to-gel transition as the temperature increased from 20 to 70 degrees C. In vitro release profiles consisted of a burst release followed a sustained release. The cytotoxicity results showed that the terpolymer micelles were biocompatible, and the encapsulated doxorubicin. HCl maintained its potent anti-tumor effect.


CONCLUSIONS
These micelles may bring the ether-anhydride family of polymers great potential as a novel carrier in nanomedicine.
Zhao
Aijun
A
Key Laboratory of Advanced Technologies of Materials Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
Zhou
Shaobing
S
Zhou
Qi
Q
Chen
Tao
T
D000812:Anhydrides / Q000138:chemical synthesis / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D002478:Cells, Cultured; D004317:Doxorubicin / Q000008:administration & dosage; D016503:Drug Delivery Systems; D004987:Ethers / Q000138:chemical synthesis / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D008823:Micelles; D020442:Poloxamer / Q000138:chemical synthesis / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D051381:Rats; D013696:Temperature
D000812:Anhydrides; D000970:Antineoplastic Agents; D004987:Ethers; D008823:Micelles; D020442:Poloxamer; D011092:Polyethylene Glycols; D004317:Doxorubicin; C514452:pluronic L64