title: [Quantity of total cell free and cell free fetal DNA in pregnancies with no complications and with preeclampsia].
abstract: Several researches focused on the factors which could influence the quantity of cell free DNA in case of normal and pathological pregnancies. The aim of our study was to evaluate the quantity of total cell free and cell free fetal DNA in case of normal pregnancies and preeclampsia.
  
  
  METHODS
  Plasma samples were obtained from 67 preeclamptic and 70 normotensive pregnant women. The quantity of total cell free DNA and cell free fetal DNA was measured using real-time polymerase chain reaction.
  
  
  RESULTS
  We confirmed that circulating total free and fetal DNA levels are significantly elevated in pregnancies complicated by preeclampsia (median: 0.0114 vs. 0.0325 and 0.001E-3 vs. 0.086E-3 ng/microl; P < 0.001). The quantity of total plasma-free DNA did not correlate with the body mass index.
  
  
  CONCLUSIONS
  The releases of both free fetal and maternal DNA were found to be affected in preeclampsia. Hepatocellular necrosis seems to be responsible - at least partly - for increased circulating total DNA levels in preeclampsia, and the abnormal trophoblast invasion could be responsible for increased trophoblast destruction and elevation of cell free fetal DNA level.
authors.affiliation: Semmelweis Egyetem, Altalános Orvostudományi Kar, I. Szülészeti és Nogyógyászati Klinika, Budapest. lazar_levente@hotmail.com
authors.forename: Levente
authors.initials: L
authors.lastname: Lázár
authors.forename: Bálint
authors.initials: B
authors.lastname: Nagy
authors.forename: Attila
authors.initials: A
authors.lastname: Molvarec
authors.forename: János
authors.initials: J
authors.lastname: Rigó
mesh_terms: D000328:Adult; D001794:Blood Pressure; D015992:Body Mass Index; D004247:DNA / Q000097:blood* / Q000302:isolation & purification; D005260:Female; D005333:Fetus / Q000378:metabolism; D006801:Humans; D016133:Polymerase Chain Reaction; D011225:Pre-Eclampsia / Q000097:blood* / Q000503:physiopathology; D011247:Pregnancy / Q000097:blood*; D012189:Retrospective Studies
chemical_list: D004247:DNA