title: Insight in the transport behavior of copper glycinate complexes through the porcine gastrointestinal membrane using an Ussing chamber assisted by mass spectrometry analysis.
abstract: An Ussing chamber study was conducted in order to investigate the transport behavior of copper glycinate complexes through a porcine gastrointestinal membrane. Organic copper complexes such as copper tri- and tetraglycinates (GGG-Cu(II) and GGGG-Cu(II)) were used as model system. In a novel analytical approach the Ussing chamber was combined with mass spectrometry. Therefore, relevant analytical methods based on MALDI-MS and a coupling of capillary electrophoresis to ICP-MS and ESI-MS were developed for the determination of copper complexes in the mucosal and serosal half-chambers. It was found that 86.1+/-8.5% of copper triglycinate but only 20.8+/-9.9% of copper tetraglycinate penetrated the digestive membrane without modification. Furthermore, inorganic copper species were not detected but a new copper complex (m/z 442) was found to be formed in both compartments of the Ussing chamber.
authors.affiliation: CNRS/Université de Pau, Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, UMR 5254, Hélioparc, 2 Av du Président Angot, F-64053 Pau, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Tastet
authors.forename: Dirk
authors.initials: D
authors.lastname: Schaumlöffel
authors.forename: Alexandros
authors.initials: A
authors.lastname: Yiannikouris
authors.forename: Ronan
authors.initials: R
authors.lastname: Power
authors.forename: Ryszard
authors.initials: R
authors.lastname: Lobinski
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods; D041981:Gastrointestinal Tract / Q000033:anatomy & histology / Q000378:metabolism; D005998:Glycine / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D009942:Organometallic Compounds / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D034421:Sus scrofa
chemical_list: D009942:Organometallic Compounds; C018493:cupric glycinate; D005998:Glycine