Relationship between polymorphonuclear leukocyte count in bronchoalveolar lavage fluid and bacterial content in Gram's stain and bacterial content in final microbiological report.
Eighty samples of bronchoalveolar lavage fluid (BALF) were obtained from the total of 48 patients (22 females and 26 males) and analyzed. Eighteen of those patients were organ transplant recipients. The relationship between polymorphonuclear leukocyte (PMN) count in direct sample and semi quantitative Gram-positive and Gram-negative bacterial content were analyzed in BALF samples. PMN count in direct sample and Gram-positive and Gram-negative bacterial content of the final microbiological report was compared as well. On the total number of samples PMN count in direct samples of BALF was statistically significant regarding the presence of Gram-positive bacteria in the same sample; it was nearly significant regarding the presence of Gram-negative bacteria; and it was statistically significant for the total bacterial content. If BALF samples are divided into those obtained from organ-transplant and those obtained from non-organ-transplant patients, positive, statistically significant relationship is found in the organ-transplant group, more specifically for the relationship between PMNs and total bacterial content. When PMN count in direct microbiological sample was compared with the results of the final microbiological report, statistically significant relationship was found neither with respect to all BALF samples, nor after dividing them into "organ-transplant" and "non-organ-transplant" group. We did not find differences caused by gender.
Cavrić
Gordana
G
Department of Internal Medicine, University Hospital "Merkur", Zagreb, Croatia. gcavric@yahoo.com
Mihalić
Slavica Naumovski
SN
Tesanović
Sanda Janković
SJ
Dvorsćak
Matea Bogdanović
MB
Erceg
Gorjana
G
Krkusek
Marijana Rehorić
MR
Bartolek
Dubravka
D
Jurić
Klara
K
Nassabain
Khaled
K
Budimir
Ivan
I
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000382:microbiology; D005260:Female; D005840:Gentian Violet; D006801:Humans; D016867:Immunocompromised Host / Q000276:immunology*; D007958:Leukocyte Count; D008172:Lung Diseases, Fungal / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology; D008297:Male; D008828:Microbiological Techniques; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology*; D016377:Organ Transplantation / Q000706:statistics & numerical data*; D010619:Phenazines; D018410:Pneumonia, Bacterial / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology; D012307:Risk Factors; D012727:Sex Characteristics; D017678:Sex Distribution; D055815:Young Adult
C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet