Quantitative determination of major phlorotannins in Ecklonia stolonifera.
Ecklonia stolonifera is a rich source of phlorotannins, which are responsible for the potent pharmacological effects associated with this seaweed. The purpose of this study was to develop a reversed-phase high-performance liquid chromatography method for the simultaneous determination of three major phlorotannins, eckol, dieckol, and phlorofucofuroeckol-A, in the extracts of Ecklonia stolonifera. The optimal chromatographic conditions were achieved on a Thermo Hypersil Gold C-18 column (250 x 4.6 mm i.d., 5 microm) using linear gradient elution of acetonitrile and water containing 0.1% formic acid at UV 254 nm. The separated phlorotannins were identified by liquid chromatography-mass spectrometry. The high-performance liquid chromatography method showed good linearity (r2 > 0.998), precision (1.4-9.5%), and accuracy (93.9-108.7%). The limits of detection ranged from 0.06 to 0.30 microg/mL and the lower limits of quantitation ranged from 0.2 to 1.0 microg/mL. Among phlorotannins, dieckol was the most abundant in both ethanol and ethyl acetate extracts of Ecklonia stolonifera.
Goo
Hyun Ryul
HR
College of Pharmacy, Kyungsung University, Busan, 608-736, Korea.
Choi
Jae Sue
JS
Na
Dong Hee
DH
D001572:Benzofurans / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004147:Dioxins / Q000302:isolation & purification*; D000459:Phaeophyceae / Q000737:chemistry*; D010946:Plants, Medicinal; D021241:Spectrometry, Mass, Electrospray Ionization; D013634:Tannins / Q000302:isolation & purification*
D001572:Benzofurans; D004147:Dioxins; D013634:Tannins; C503840:dieckol; C064093:phlorofucofuroeckol A; C060311:eckol