Differential diagnosis of liver diseases using serum biomarkers.
OBJECTIVE
Many human blood proteins are synthesised in the liver. Their serum levels may decrease or increase due to liver disorders and some of them serve as useful biomarkers. Determination of serum concentration of different biomarkers has important role in diagnosis of liver diseases and in monitoring the course of disease. In this work 3 serum markers associated with liver disorders were compared. The aim was to assess whether these biomarkers exhibit specific distribution pattern in different types of liver disease: liver neoplasia (primary hepatocellular carcinoma [HC] or metastatic liver disease [MLD] from colon cancer), viral hepatitis C (HCV) and the parasitic infection echinococcosis.


METHODS
Serum concentrations of alpha-fetoprotein (AFP), ferritin and insulin-like growth factor I (IGF-I) were determined in patients with liver disease and compared between patient groups and with healthy persons.


RESULTS
Serum AFP and ferritin levels exhibited similar pattern of change in patients with liver neoplasia or HCV, and concentrations of these 2 markers were significantly increased compared to the control group (p < 0.01 in each case). On the other hand, the concentration of IGF-I was significantly decreased in patients with liver neoplasia or echinococcosis compared to the control group (p < 0.05 for both). The concentration of IGF-I was significantly lower and the concentration of ferritin significantly higher in patients with HC than in patients with MLD from colorectal cancer (p < 0.01 for both).


CONCLUSIONS
The results have shown that each hepatic pathology studied exhibited specific profile of the analysed set of biomarkers. Therefore, the simultaneous determination of the 3 mentioned biomarkers may help in differential diagnosis of liver diseases.
Cujic
D
D
Institute for the Application of Nuclear Energy-INEP, University of Belgrade, Belgrade, Zemun, Serbia. danicac@inep.co.rs
Golubovic
S
S
Bojic-Trbojevic
Z
Z
Ilic
N
N
Baricevic
I
I
Nedic
O
O
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000175:diagnosis / Q000556:secondary; D015179:Colorectal Neoplasms / Q000473:pathology; D003937:Diagnosis, Differential; D004444:Echinococcosis, Hepatic / Q000097:blood / Q000175:diagnosis; D005260:Female; D005293:Ferritins / Q000097:blood*; D006509:Hepatitis B / Q000097:blood / Q000175:diagnosis; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D008107:Liver Diseases / Q000097:blood / Q000175:diagnosis*; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis / Q000556:secondary; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D055815:Young Adult; D000509:alpha-Fetoproteins / Q000032:analysis*
C097353:AFP protein, human; D015415:Biomarkers; D000509:alpha-Fetoproteins; D007334:Insulin-Like Growth Factor I; D005293:Ferritins