title: Capacitive micromachined ultrasound transducer (cMUT) for immunosensor design.
abstract: An affinity sensor based on a capacitive micromachined ultrasound transducer (cMUT) is reported by this Communication. The cMUT micromembrane arrays modified with adsorbed bovine leukemia virus protein gp51 were applied as a biological recognition part. The cMUT-based sensor is shown to be sensitive to the antibodies against bovine leukemia virus protein gp51 (anti-gp51). Two different concentrations of anti-gp51-containing samples and one blank sample without anti-gp51 were tested. The sensitivity of cMUT-based immunosensor is comparable with the sensitivity of a quartz microbalance-based immunosensor. The cMUT array provides a multi-channel system for the measurement of analytical signal. Moreover, two different characteristics--the resonance frequency shift (Deltaf) and the shift of the real part of the electromechanical impedance (DeltaRe)--could have been evaluated simultaneously. Both analytical signals are informative and can be applied for the estimation of immune complex formation. We found the performance of such a system being potentially superior over some other immunosensing techniques. It is more rapid than electrochemical techniques and provides two different informative parameters.
authors.affiliation: NanoTechnas-Centre of Nanotechnology and Material Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania.
authors.forename: Almira
authors.initials: A
authors.lastname: Ramanaviciene
authors.forename: Darius
authors.initials: D
authors.lastname: Virzonis
authors.forename: Gailius
authors.initials: G
authors.lastname: Vanagas
authors.forename: Arūnas
authors.initials: A
authors.lastname: Ramanavicius
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002417:Cattle; D004867:Equipment Design; D001909:Leukemia Virus, Bovine / Q000276:immunology; D014465:Ultrasonics; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014764:Viral Proteins