title: [Screen of a membrane protein molecule from Vero cells capable of binding Japanese encephalitis virus(JEV)].
abstract: OBJECTIVE
  Identification of membrane protein molecules Vero cells binding JEV.
  
  
  METHODS
  Membrane protein extract was subject to co-immunoprecipitation (Co-IP) with JEV, and identified by mass (MS) spectrometry. The binding between specific extract protein and JEV was measured by MS, flow cytometry (FCM) and immunofluorescence assay (IFA).
  
  
  RESULTS
  Heat shock protein 90 beta (HSP90 beta) was identified by Co-IP and MS, and its binding JEV activity was confirmed by FCM and IFA.
  
  
  CONCLUSIONS
  HSP 90 beta from Vero cells membrane could bind JEV.
authors.affiliation: Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.forename: Tian-bing
authors.initials: TB
authors.lastname: Ding
authors.forename: Zhi-kai
authors.initials: ZK
authors.lastname: Xu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D002522:Chlorocebus aethiops; D004664:Encephalitis Virus, Japanese / Q000378:metabolism*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D018841:HSP90 Heat-Shock Proteins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding; D014709:Vero Cells
chemical_list: D000911:Antibodies, Monoclonal; D018841:HSP90 Heat-Shock Proteins; C493165:HSP90AB1 protein, human; D008565:Membrane Proteins