Schistosoma mansoni Annexin 2: molecular characterization and immunolocalization.
We here describe the cloning and characterization of the Schistosoma mansoni Annexin 2, previously identified in the tegument by proteomic studies, and as an up-regulated gene in schistosomulum stage by microarray data. In silico analysis predicts a conserved core containing four repeat domains of Annexin (ANX) and a variable N-terminal region similar to that described for mammalian isoforms. Real-time RT-PCR and Western blot analysis determined that S. mansoni Annexin 2 is significantly up-regulated in the transition from free-living cercaria to schistosomulum and adult worm parasitic stages. Immunolocalization experiments and tegument membrane preparations confirmed Annexin 2 as a protein mainly localized in the tegument of schistosomula and adult worms. Furthermore, it binds to the tegument surface membranes in a calcium-dependent manner. These results suggest that S. mansoni Annexin 2 is closely associated to the tegument arrangement, being a potential target for immune intervention.
Tararam
Cibele Aparecida
CA
Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil, 1500, São Paulo, SP, Brazil.
Farias
Leonardo Paiva
LP
Wilson
R Alan
RA
Leite
Luciana Cezar de Cerqueira
LC
D000595:Amino Acid Sequence; D000818:Animals; D017306:Annexin A2 / Q000032:analysis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000909:Antibodies, Helminth / Q000096:biosynthesis; D015153:Blotting, Western; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D008018:Life Cycle Stages / Q000235:genetics; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012550:Schistosoma mansoni / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D016415:Sequence Alignment
D017306:Annexin A2; D000909:Antibodies, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth