title: Endoplasmic reticulum stress response is involved in Mycobacterium tuberculosis protein ESAT-6-mediated apoptosis.
abstract: Mycobacterium tuberculosis (Mtb) infection leads to the induction of the apoptotic response, which is associated with bacilli killing. The early secreted mycobacterial antigen ESAT-6 of Mtb has been shown to induce apoptosis in human macrophages and epithelial cells. In the present study, we demonstrate that the stimulation of human epithelial A549 cells by ESAT-6 induces the endoplasmic reticulum (ER) stress response. We observed that ESAT-6 treatment increases intracellular Ca(2+) concentration, which results in ROS accumulation, and therefore induces the onset of ER stress-induced apoptosis. Our results uncover a novel apoptotic mechanism of ESAT-6 through ER stress responses in pathologic conditions such as tuberculosis.
authors.affiliation: Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, South Korea.
authors.forename: Hong-Hee
authors.initials: HH
authors.lastname: Choi
authors.forename: Dong-Min
authors.initials: DM
authors.lastname: Shin
authors.forename: Gun
authors.initials: G
authors.lastname: Kang
authors.forename: Ki-Hye
authors.initials: KH
authors.lastname: Kim
authors.forename: Jin Bong
authors.initials: JB
authors.lastname: Park
authors.forename: Gang Min
authors.initials: GM
authors.lastname: Hur
authors.forename: Hye-Mi
authors.initials: HM
authors.lastname: Lee
authors.forename: Yun-Ji
authors.initials: YJ
authors.lastname: Lim
authors.forename: Jeong-Kyu
authors.initials: JK
authors.lastname: Park
authors.forename: Eun-Kyeong
authors.initials: EK
authors.lastname: Jo
authors.forename: Chang-Hwa
authors.initials: CH
authors.lastname: Song
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism*; D017209:Apoptosis; D001426:Bacterial Proteins / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014376:Tuberculosis / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D011506:Proteins; D017382:Reactive Oxygen Species