Recombinant Mycobacterium bovis BCG expressing chimaeric protein of Ag85B and ESAT-6 enhances immunostimulatory activity of human macrophages.
Recombinant BCG strain that secretes the chimaeric protein of Ag85B and ESAT-6 has been demonstrated to augment Th1 immune response in C57BL/6 mice. In this paper, we studied the immunostimulatory activity of the recombinant BCG strains in vitro and found out that rBCG-A(N)-E-A(C) activated THP-1 cells and induced higher expression levels of CD86, CD80, CD40 and HLA-DR, especially increased the ratio of CD86/CD80. Likewise, rBCG-A(N)-E-A(C) infection was able to stimulate an increase in TNF-alpha production of macrophages. Moreover, rBCG-A(N)-E-A(C) up-regulated the expression of EFHD2, ACTB and ACTG1 in the macrophages and improved the ability of antigen presentation and the CD8(+) T-cells immune response. Taken together, this rBCG-A(N)-E-A(C) strain enhanced the immunostimulatory activity of human macrophages and could be a potential vaccine against Mycobacterium tuberculosis.
Xu
Ying
Y
State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, China.
Liu
Wei
W
Shen
Hongbo
H
Yan
Jingran
J
Yang
Enzhuo
E
Wang
Honghai
H
D000199:Actins / Q000096:biosynthesis; D000217:Acyltransferases / Q000235:genetics / Q000276:immunology*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D015703:Antigens, CD / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D002135:Calcium-Binding Proteins / Q000096:biosynthesis; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D009163:Mycobacterium bovis / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D032581:Tuberculosis Vaccines / Q000235:genetics / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000199:Actins; D000942:Antigens, Bacterial; D015703:Antigens, CD; D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; C502299:EFHD2 protein, human; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D006684:HLA-DR Antigens; D011994:Recombinant Proteins; D032581:Tuberculosis Vaccines; D014409:Tumor Necrosis Factor-alpha; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis