title: [Influence of gemcitabine on expression of C-myc gene and its apoptosis-inducing effect on HL-60 cells].
abstract: The aim of this study was to investigate the effects of gemcitabine(GEM) on apoptosis and c-myc gene expression of HL-60 cells, and feasibility of using GEM in therapy of leukemia. The HL-60 cells were cultured in vitro. The expressions of the c-myc mRNA and C-MYC protein were detected by RT-PCR and Western-blot respectively. The cell apoptosis was analyzed by TUNEL staining. The results showed that after the HL-60 cells were treated with 1.0 microg/ml GEM for 12, 24, 36 and 48 hours, the expression of c-myc mRNA was inhibited to various degree. This inhibitory effect displayed time-dependent manner and the most optimal effective time was 24 hours. Compared GEM group with Ara-C group and blank control group, there were statistical differences (p<0.05). After the HL-60 cells were treated with 1.0 microg/ml GEM for 24, 48, 72 hours, C-MYC protein significantly decreased, and the expression of C-MYC protein reached to lowest level at 48 hours after treating with GEM, and with inhibition rate of 94.16%. Compared GEM group with Ara-C group and blank control group, the differences were significant (p<0.01). There was significant difference between cells treated with GEM for 24, 48 and 72 hours (p<0.01). After the HL-60 cells were treated with 1.0 microg/ml GEM for 24 hours, the apoptotic cells increased obviously. The positive rate was 83.67% in GEM-treated group. Compared GEM group with Ara-C group (positive rate 10.67%) and untreated group (positive rate 3.00%), the differences had statistical significance (p<0.01). It is concluded that GEM can induce the apoptosis and down-regulate c-myc gene expression significantly in HL-60 cells and it may be used as a new therapeutic drug for leukemia.
authors.affiliation: Department of Hematology, The First Affiliated Hospital, and Centern for Stem Cell REsearch, Zhengzhou University, Zhengzhou 450052, Henan Province, China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Gong
authors.forename: Ling
authors.initials: L
authors.lastname: Sun
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Ren
authors.forename: Liu
authors.initials: L
authors.lastname: Liu
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Han
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D000093542:Gemcitabine
chemical_list: D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D003841:Deoxycytidine; D000093542:Gemcitabine