title: [A comparative study on HPLC fingerprint of Semen Strychni and the processed].
abstract: OBJECTIVE
  To establish the fingerprint of Semen Strychni and the processed by HPLC.
  
  
  METHODS
  The HPLC Method was used, the chromatgraphic conditions were as follows: Chromatographic column: Lichrospher C18 (250 mm x 4.6 mm, 5 microm), mobile phase: solvent A consisted of water-ethanoic acid-triethylamine (100: 0.2: 0.2), solvent B contained acetonitril (gradient elution), column temperature: 30 degrees C, flow rate: 1.0 mL/min, detection wavelength: 254 nm.
  
  
  RESULTS
  The similar degree of 10 batch samples buiding sharing mode of crude drug and processed product was above 0.9. 18 common peaks in chromatograms were separated from 10 batches of Semen Strychni samples and 21 common peaks in chromatograms were separated from 10 batches of the processed.
  
  
  CONCLUSIONS
  This study establishes the HPLC fingerprint commonmode, and researches the difference between Semen Strychni and the processed in HPLC fingerprint.
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of TCM, Nanjing 210029, China. lijunsong1964@163.com
authors.forename: Jun-song
authors.initials: JS
authors.lastname: Li
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Wang
authors.forename: Bao-chang
authors.initials: BC
authors.lastname: Cai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D010598:Pharmacognosy; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D013331:Strychnine / Q000031:analogs & derivatives / Q000032:analysis; D031451:Strychnos / Q000737:chemistry* / Q000254:growth & development
chemical_list: D004365:Drugs, Chinese Herbal; C083806:brucine; D013331:Strychnine