title: Developmental iodine deficiency and hypothyroidism impair neural development in rat hippocampus: involvement of doublecortin and NCAM-180.
abstract: BACKGROUND
  Developmental iodine deficiency results in inadequate thyroid hormone (TH), which damages the hippocampus. Here, we explored the roles of hippocampal doublecortin and neural cell adhesion molecule (NCAM)-180 in developmental iodine deficiency and hypothyroidism.
  
  
  METHODS
  Two developmental rat models were established with either an iodine-deficient diet, or propylthiouracil (PTU)-adulterated water (5 ppm or 15 ppm) to impair thyroid function, in pregnant rats from gestational day 6 until postnatal day (PN) 28. Silver-stained neurons and protein levels of doublecortin and NCAM-180 in several hippocampal subregions were assessed on PN14, PN21, PN28, and PN42.
  
  
  RESULTS
  The results show that nerve fibers in iodine-deficient and 15 ppm PTU-treated rats were injured on PN28 and PN42. Downregulation of doublecortin and upregulation of NCAM-180 were observed in iodine-deficient and 15 ppm PTU-treated rats from PN14 on. These alterations were irreversible by the restoration of serum TH concentrations on PN42.
  
  
  CONCLUSIONS
  Developmental iodine deficiency and hypothyroidism impair the expression of doublecortin and NCAM-180, leading to nerve fiber malfunction and thus impairments in hippocampal development.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, China Medical University, Shenyang, PR China. chenjie@mail.cmu.edu.cn
authors.forename: Jian
authors.initials: J
authors.lastname: Gong
authors.forename: Wanyang
authors.initials: W
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Hongde
authors.initials: H
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Jiapeng
authors.initials: J
authors.lastname: Zhong
authors.forename: Qi
authors.initials: Q
authors.lastname: Xi
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000378:metabolism / Q000473:pathology; D001928:Brain Diseases, Metabolic / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D004195:Disease Models, Animal; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D015536:Down-Regulation / Q000502:physiology; D006624:Hippocampus / Q000254:growth & development* / Q000378:metabolism* / Q000503:physiopathology; D007037:Hypothyroidism / Q000150:complications*; D007455:Iodine / Q000172:deficiency*; D007859:Learning Disabilities / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology; D009479:Neuropeptides / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013963:Thyroid Hormones / Q000096:biosynthesis / Q000097:blood; D015854:Up-Regulation / Q000502:physiology
chemical_list: C000714848:Dcx protein, rat; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D019006:Neural Cell Adhesion Molecules; D009479:Neuropeptides; D013963:Thyroid Hormones; D007455:Iodine