Multiplex identification of microbes.
We have adapted molecular inversion probe technology to identify microbes in a highly multiplexed procedure. This procedure does not require growth of the microbes. Rather, the technology employs DNA homology twice: once for the molecular probe to hybridize to its homologous DNA and again for the 20-mer oligonucleotide barcode on the molecular probe to hybridize to a commercially available molecular barcode array. As proof of concept, we have designed, tested, and employed 192 molecular probes for 40 microbes. While these particular molecular probes are aimed at our interest in the microbes in the human vagina, this molecular probe method could be employed to identify the microbes in any ecological niche.
Hyman
Richard W
RW
Department of Biochemistry, Stanford Genome Technology Center, Stanford University Medical School, 855 California Avenue, Palo Alto, California 94304, USA. rhyman@stanford.edu
St Onge
Robert P
RP
Allen
Edward A
EA
Miranda
Molly
M
Aparicio
Ana Maria
AM
Fukushima
Marilyn
M
Davis
Ronald W
RW
D001431:Bacteriological Techniques / Q000379:methods*; D003141:Communicable Diseases / Q000175:diagnosis*; D005260:Female; D006801:Humans; D015336:Molecular Probe Techniques; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014621:Vagina / Q000382:microbiology