title: Purification of coagulation factor VIII using chromatographic methods. Direct chromatography of plasma in anion exchange resins.
abstract: Coagulation factor VIII (FVIII) concentrates are used in the treatment of patients with Hemophilia A. Human FVIII was purified directly from plasma using anion exchange chromatography followed by gel filtration. Three Q-Sepharose resins were tested, resulting in 40% recovery of FVIII activity using Q-Sepharose XL resin, about 80% using Q-Sepharose Fast Flow and 70% using the Q-Sepharose Big Beads. The vitamin K-dependent coagulation factors co-eluted with FVIII from the anion exchange columns. In the second step of purification, when Sepharose 6FF was used, 70% of FVIII activity was recovered free from vitamin K-dependent factors.
authors.affiliation: Centro de Biotecnologia, Instituto Butantan, São Paulo, Brazil.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Cheng
authors.forename: Daniela
authors.initials: D
authors.lastname: Jinzenji
authors.forename: Ana Paula Almeida Aranha
authors.initials: AP
authors.lastname: Lorthiois
authors.forename: Roberta Rodrigues
authors.initials: RR
authors.lastname: de Carvalho
authors.forename: Anita Mitiko
authors.initials: AM
authors.lastname: Tanaka-Azevedo
authors.forename: Isaías
authors.initials: I
authors.lastname: Raw
authors.forename: Elizabeth Angélica Leme
authors.initials: EA
authors.lastname: Martins
mesh_terms: D000837:Anion Exchange Resins; D001709:Biotechnology / Q000379:methods*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange / Q000379:methods; D005169:Factor VIII / Q000302:isolation & purification*; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000837:Anion Exchange Resins; C078147:F8 protein, human; D005169:Factor VIII