Inhibitory effect of caffeic acid phenethyl ester on mice bearing tumor involving angiostatic and apoptotic activities.
This study aims at investigating the anti-tumor effect of caffeic acid phenethyl ester (CAPE) against animal carcinogenesis. In order to substantiate this fact implanted tumor Ehrlich carcinoma cells were assessed in vivo to Swiss mice strain. We found that administrating of CAPE (15 mg/kg S.C.) showed that the tumor volume decreased significantly by 51%. As a result, it improved animal chances of survival and they became healthier. An anti-angiogenic effect of CAPE in vivo was observed, as determined by a significant serum matrix metalloproteinase (MMP-9) reduction (142.1 ng/ml), activation of endostatin serum level (1.9 ng/ml), as well as DNA fragmentation in tumor treated mice when compared with untreated ones.


CONCLUSIONS
CAPE has a significant inhibitory effect on tumor in vivo. This inhibition may be related to its angiostatic and apoptotic effects. It also reduced angiogenic factors which may shift the equilibrium to the angiostatic effect of CAPE. These findings provide the possibility for the future use of CAPE as tumor therapy in human clinical trials.
El-Refaei
Mohamed F
MF
Molecular Biology Department, Genetic Engineering and Biotechnology Institute, Menoufiya University, Egypt. melrefaei2000@yahoo.com
El-Naa
Mona M
MM
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002109:Caffeic Acids / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000097:blood / Q000098:blood supply / Q000188:drug therapy* / Q000473:pathology; D053938:DNA Fragmentation / Q000187:drug effects; D043169:Endostatins / Q000097:blood; D005260:Female; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000097:blood; D051379:Mice; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology
D000970:Antineoplastic Agents; D002109:Caffeic Acids; D043169:Endostatins; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol