title: YieJ (CbrC) mediates CreBC-dependent colicin E2 tolerance in Escherichia coli.
abstract: Colicin E2-tolerant (known as Cet2) Escherichia coli K-12 mutants overproduce an inner membrane protein, CreD, which is believed to cause the Cet2 phenotype. Here, we show that overproduction of CreD in a Cet2 strain results from hyperactivation of the CreBC two-component regulator, but CreD overproduction is not responsible for the Cet2 phenotype. Through microarray analysis and gene knockout and overexpression studies, we show that overexpression of another CreBC-regulated gene, yieJ (also known as cbrC), causes the Cet2 phenotype.
authors.affiliation: Department of Cellular and Molecular Medicine, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom.
authors.forename: S James L
authors.initials: SJ
authors.lastname: Cariss
authors.forename: Chrystala
authors.initials: C
authors.lastname: Constantinidou
authors.forename: Mala D
authors.initials: MD
authors.lastname: Patel
authors.forename: Yuiko
authors.initials: Y
authors.lastname: Takebayashi
authors.forename: Jon L
authors.initials: JL
authors.lastname: Hobman
authors.forename: Charles W
authors.initials: CW
authors.lastname: Penn
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Avison
mesh_terms: D003087:Colicins / Q000494:pharmacology*; D048168:Escherichia coli K12 / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D003087:Colicins; D029968:Escherichia coli Proteins