title: Lipopolysaccaride-binding peptides obtained by phage display method.
abstract: Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria. It has strong toxicity and might cause sepsis or septic shock. Thus early detection of LPS and neutralization of LPS toxicity are required. We obtained several new LPS-binding peptides using a phage display method. We synthesized 3 of these peptides and analyzed their binding affinity and capacity to LPS. One of these peptides, named Li5-001, showed high binding affinity to LPS and lipid A; the K(d) values were 10 and 1 nM, respectively. Li5-001 showed a high binding capacity to LPS, and was estimated to bind 130 ng LPS/mg, which is higher than that of polymyxin B (80 ng LPS/mg); however, its LPS-neutralizing activity was low. Li5-001 coupled with beads will be useful for eliminating endotoxin contamination from pharmaceuticals. Its low LPS-neutralizing activity allows to be used in the Limulus amebocyte lysate test without eluting LPS from the Li5-001 coupled beads.
authors.affiliation: Peptide Door Co. Ltd., Sangyo-sozo-kan 204, Shimonojo-machi 936-14, Takasaki, Gunma 370-0854, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Horiuchi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Masaki
authors.initials: M
authors.lastname: Ochiai
authors.forename: Makoto
authors.initials: M
authors.lastname: Niwa
authors.forename: Takashi
authors.initials: T
authors.lastname: Takagi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Omi
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Masatsugu-Matt
authors.initials: MM
authors.lastname: Suzuki
mesh_terms: D001681:Biological Assay / Q000379:methods*; D004731:Endotoxins / Q000737:chemistry* / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000737:chemistry* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides; D019151:Peptide Library; D010455:Peptides