title: Whole-animal senescent cytotoxic T cell removal using antibodies linked to magnetic nanoparticles.
abstract: A major type of unwanted cells that accumulate in aging are anergic cytotoxic T cells. These cells often have virus-specific T cell receptors, as well as other surface markers that distinguish them from their youthful counterparts, and they are thought to play a major role in the decline of the immune system with age. Here we consider two surface markers thought to define these cells in mice, CD8 and Killer cell lectin-like receptor G1 (KLRG1), and a means we developed to remove these cells from the blood of aged C57BL/6 mice. Using antibodies with magnetic nanoparticles linked to their Fc domains, we first developed a method to use magnets to filter out the unwanted cells from the blood and later constructed a device that does this automatically. We demonstrated that this device could reduce the KLRG1-positive CD8 cell count in aged mouse blood by a factor of 7.3 relative to the total CD8 cell compartment, reaching a level typically seen only in very young animals.
authors.affiliation: SENS Foundation Research Center, Sunnyvale, California, USA. justinrebo@eternaltraveler.org
authors.forename: Justin
authors.initials: J
authors.lastname: Rebo
authors.forename: Keith
authors.initials: K
authors.lastname: Causey
authors.forename: Ben
authors.initials: B
authors.lastname: Zealley
authors.forename: Tim
authors.initials: T
authors.lastname: Webb
authors.forename: Mark
authors.initials: M
authors.lastname: Hamalainen
authors.forename: Brian
authors.initials: B
authors.lastname: Cook
authors.forename: John
authors.initials: J
authors.lastname: Schloendorn
mesh_terms: D000375:Aging / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000961:Antilymphocyte Serum / Q000737:chemistry / Q000627:therapeutic use*; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology; D002469:Cell Separation / Q000379:methods; D016238:Cytapheresis / Q000379:methods; D037181:Lectins, C-Type; D008280:Magnetics; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000473:pathology*
chemical_list: D000961:Antilymphocyte Serum; C451496:Klrg1 protein, mouse; D037181:Lectins, C-Type; D011971:Receptors, Immunologic