title: Kinetics of lipase catalyzed isoamyl acetate synthesis at high hydrostatic pressure in hexane.
abstract: Lipase-catalyzed synthesis of isoamyl acetate in hexane at 10-250 MPa at 80 degrees C and 1-100 MPa at 40 degrees C resulted in activation volumes of -12.9 +/- 1.7 and -21.6 +/- 2.9 cm(3) mol(-1), respectively. Increasing pressure from 10 to 200 MPa resulted in approximately 10-fold increase in V(max) at both 40 and 80 degrees C. Pressure increased the K(m) from 2.4 +/- 0.004 to 38 +/- 0.78 mM at 40 degrees C. In contrast, at 80 degrees C the pressure did not affect the K(m).
authors.affiliation: Department of Food Science and Human Nutrition, University of Florida/IFAS, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Eisenmenger
authors.forename: José I
authors.initials: JI
authors.lastname: Reyes-De-Corcuera
mesh_terms: D006586:Hexanes / Q000378:metabolism*; D006874:Hydrostatic Pressure; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D000439:Pentanols / Q000378:metabolism*; D013696:Temperature
chemical_list: D006586:Hexanes; D000439:Pentanols; D008049:Lipase; C020377:isoamyl acetate