title: Quantitation of virus using laser-based scanning of near-infrared fluorophores replaces manual plate reading in a virus titration assay.
abstract: A method was developed for quantitation of a virus titration assay for minimally cytopathic and noncytopathic viruses that utilizes laser-based scanning of near-infrared (NIR) fluorophores. This automated method bypasses the need for manual plate reading thus eliminating human bias and error. The image data is translated by LI-COR's Odyssey software into numerical data which is used directly in the virus titer calculations.
authors.affiliation: LI-COR Biosciences, Lincoln, NE 68504, USA. sally.weldon@licor.com
authors.forename: Sally K
authors.initials: SK
authors.lastname: Weldon
authors.forename: Shawn L
authors.initials: SL
authors.lastname: Mischnick
authors.forename: Teresa M
authors.initials: TM
authors.lastname: Urlacher
authors.forename: Kristi L H
authors.initials: KL
authors.lastname: Ambroz
mesh_terms: D000818:Animals; D001331:Automation / Q000379:methods*; D002460:Cell Line; D028301:Feline Panleukopenia Virus / Q000302:isolation & purification*; D007834:Lasers; D019562:Viral Load / Q000379:methods*