Reporter alleles that inform on differences in Cre recombinase expression.
Alleles that express reporters after Cre recombination allow for fate-mapping studies when used in combination with appropriate cre alleles. In this study, we describe two fluorescent reporter alleles that differentially mark populations of cells as a function of their level of expression of Cre recombinase. Mice carrying these alleles were generated and used to demonstrate the usefulness of the reporter alleles for informing on prior Cre recombinase expression in lymphocytes. The alleles expand the range of genetic tools available for understanding how differences in gene expression result in divergent developmental fates during the development and differentiation of lymphocytes and other cells.
Klinger
Mark
M
Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143-0414, USA.
Chmura
Stephen A
SA
Killeen
Nigel
N
D000483:Alleles; D000818:Animals; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D017930:Genes, Reporter / Q000235:genetics*; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D019426:Integrases / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D014162:Transfection
D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases