Systemic transplantation of embryonic stem cells accelerates brain lesion decrease and angiogenesis.
As stem cells can regenerate damaged tissue, their therapeutic potential on brain damage has been investigated. In this study, the effects of embryonic stem cell transplantation on brain damage were investigated by using a photochemically induced thrombotic brain damage model. Mice with systemic transplantation of embryonic stem cells expressing enhanced green fluorescence protein on day 1 showed a smaller brain lesion size on day 8 than the control mice. The smaller lesion was accompanied by the increase in the number of microvessels at the border of the damaged area. Inside and around the damaged lesion, no EGFP-positive cells were observed. These findings suggested that embryonic stem cell transplantation reduced the brain lesion through the acceleration of angiogenesis by endogenous endothelial cells.
Nagai
Nobuo
N
Department of Physiology, Kinki University School of Medicine, Osaka, Japan.
Kawao
Naoyuki
N
Okada
Kiyotaka
K
Okumoto
Katsumi
K
Teramura
Takeshi
T
Ueshima
Shigeru
S
Umemura
Kazuo
K
Matsuo
Osamu
O
D000818:Animals; D001929:Brain Edema / Q000209:etiology / Q000503:physiopathology / Q000601:surgery; D020520:Brain Infarction / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D002196:Capillaries / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques; D002460:Cell Line; D004195:Disease Models, Animal; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D005456:Fluorescent Dyes / Q000528:radiation effects / Q000633:toxicity; D020767:Intracranial Thrombosis / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008833:Microcirculation / Q000502:physiology; D009369:Neoplasms / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology*; D010775:Photic Stimulation / Q000009:adverse effects; D011183:Postoperative Complications / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D018570:Risk Assessment; D012395:Rose Bengal / Q000528:radiation effects / Q000633:toxicity; D033581:Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D014184:Transplantation, Homologous / Q000009:adverse effects / Q000379:methods
D005456:Fluorescent Dyes; D012395:Rose Bengal