[Screen of a membrane protein molecule from Vero cells capable of binding Japanese encephalitis virus(JEV)].
OBJECTIVE
Identification of membrane protein molecules Vero cells binding JEV.


METHODS
Membrane protein extract was subject to co-immunoprecipitation (Co-IP) with JEV, and identified by mass (MS) spectrometry. The binding between specific extract protein and JEV was measured by MS, flow cytometry (FCM) and immunofluorescence assay (IFA).


RESULTS
Heat shock protein 90 beta (HSP90 beta) was identified by Co-IP and MS, and its binding JEV activity was confirmed by FCM and IFA.


CONCLUSIONS
HSP 90 beta from Vero cells membrane could bind JEV.
Li
Yan
Y
Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China.
Shi
Ying
Y
Ding
Tian-bing
TB
Xu
Zhi-kai
ZK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D002522:Chlorocebus aethiops; D004664:Encephalitis Virus, Japanese / Q000378:metabolism*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D018841:HSP90 Heat-Shock Proteins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D018841:HSP90 Heat-Shock Proteins; C493165:HSP90AB1 protein, human; D008565:Membrane Proteins