The apolipoprotein A-I level is downregulated in the granulosa cells of patients with polycystic ovary syndrome and affects steroidogenesis.
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder found in women. The etiology of PCOS is still not clear, and there are no available studies on the proteome analysis of granulosa cells (GCs) in PCOS patients. To identify the pathogenic mechanisms and potential diagnostic markers for PCOS, we conducted proteomic profiling of GCs in PCOS patients by two-dimensional gel electrophoresis and liquid chromatography coupled with mass spectrometry (LC-MS/MS) analyses. The proteomic analysis yielded eight downregulated and 12 upregulated proteins in PCOS patients, among which apolipoprotein A-I (ApoA-I) showed significant downregulation in PCOS patients as confirmed by Western blotting. Knockdown of ApoA-I decreased the number of transcripts of steroidogenic enzymes in a granulosa cell line (KGN), while its overexpression generally increased the level of expression of these enzymes. Furthermore, modulation of the expression level of ApoA-I in the granulosa cells altered progesterone production. Therefore, this study suggests that ApoA-I can be useful as a granulosa cell biomarker of PCOS patients and that downregulated ApoA-I may be related to the disturbed production of steroid hormones in PCOS patients.
Choi
Dong-Hee
DH
Department of Obstetrics and Gynecology, Bundang CHA Women's Hospital, and Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea 463-836.
Lee
Woo-Sik
WS
Won
Miae
M
Park
Mira
M
Park
Ho-Oak
HO
Kim
Eunju
E
Lee
Kyoung-Ah
KA
Bae
Jeehyeon
J
D000328:Adult; D016632:Apolipoprotein A-I / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D002853:Chromatography, Liquid; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D011085:Polycystic Ovary Syndrome / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011374:Progesterone / Q000096:biosynthesis* / Q000378:metabolism; D020543:Proteome / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
D016632:Apolipoprotein A-I; D020543:Proteome; D011374:Progesterone