Note: Dynamic point spread function for single and multiphoton fluorescence microscopy.
We propose and demonstrate a dynamic point spread function (PSF) for single and multiphoton fluorescence microscopy. The goal is to generate a PSF whose shape and size can be maneuvered from highly localized to elongated one, thereby allowing shallow-to-depth excitation capability during active imaging. The PSF is obtained by utilizing specially designed spatial filter and dynamically altering the filter parameters. We predict potential applications in nanobioimaging and fluorescence microscopy.
Mondal
Partha Pratim
PP
Department of Instrumentation, Indian Institute of Science, Bangalore 560012, India. partha@isu.iisc.ernet.in
Mandal
Subhra
S
Diaspro
Alberto
A
D000465:Algorithms; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*