Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.
Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front of a bright background. Here, we show this effect on a three-dimensional image stack of a hippocampus sample, focusing on the CA1 region. This method, called FIF-Ultramicroscopy, allows for the three-dimensional observation of cellular structures in various tissue types without complicated staining techniques.
Leischner
Ulrich
U
Max Planck Institute of Psychiatry, Munich, Germany. Ulrich.leischner@lrz.tum.de
Schierloh
Anja
A
Zieglgänsberger
Walter
W
Dodt
Hans-Ulrich
HU
D000465:Algorithms; D000818:Animals; D048430:Cell Shape; D005453:Fluorescence; D005557:Formaldehyde / Q000737:chemistry*; D006624:Hippocampus / Q000166:cytology; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000379:methods
D005557:Formaldehyde