Changes in mitochondrial respiratory enzyme activity after ischemia-reperfusion injury in living-donor liver transplantation.
BACKGROUND
Ischemia-reperfusion (I-R) injury plays an important role in the immediate graft function in living-donor liver transplantation (LDLT). There is growing evidence that mitochondria play a pivotal role in I-R injury. Our aim was to evaluate changes in mitochondrial respiratory enzyme activities after I-R injury in LDLT.


METHODS
Specimens from 8 donor recipient pairs enrolled in this study were obtained from the donor livers before harvest (before I-R injury) and after vascular anastomosis in the recipient (after I-R injury). Histidine-tryptophan-ketoglutarate solution was used to perfuse the organ during the cold ischemic period between harvesting and transplantation. We correlated changes in mitochondrial respiratory enzyme complex activity (succinate cytochrome c reductase [SCCR]; NADH cytochrome c reductase [NCCR]) after I-R injury with clinical data and graft status.


RESULTS
NCCR and SCCR activities did not uniformly decrease after I-R injury. Two of 8 recipients experienced graft dysfunction after transplantation. The decrease in neither NCCR nor SCCR activity correlated with graft dysfunction in these 2 patients. Among the clinical factors, grafts from older donors tended to show decreased NCCR activity after I-R injury.


CONCLUSIONS
In this study, changes in mitochondrial respiratory enzyme activity failed to predict the severity of I-R injury in LDLT. The organ preservation solution may play a protective role on mitochondrial respiratory enzymes during I-R injury.
Lin
N-C
NC
Division of Transplantation Surgery, Department of Surgery, Taipei Veterans General Hospital, National Yang-Ming University School of Medicine, Taipei, Taiwan.
Liu
C-S
CS
Chang
C-J
CJ
Loong
C-C
CC
Hsia
C-Y
CY
Tsai
H-L
HL
D000328:Adult; D000367:Age Factors; D000368:Aged; D015415:Biomarkers; D005260:Female; D006801:Humans; D007700:Kinetics; D016031:Liver Transplantation / Q000009:adverse effects*; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D008930:Mitochondria, Liver / Q000201:enzymology*; D009245:NADH Dehydrogenase / Q000378:metabolism*; D015427:Reperfusion Injury / Q000201:enzymology*; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism*; D017211:Treatment Failure; D016896:Treatment Outcome
D015415:Biomarkers; D013384:Succinate Cytochrome c Oxidoreductase; D009245:NADH Dehydrogenase