title: The fusion kinase ITK-SYK mimics a T cell receptor signal and drives oncogenesis in conditional mouse models of peripheral T cell lymphoma.
abstract: Peripheral T cell lymphomas (PTCLs) are highly aggressive malignancies with poor prognosis. Their molecular pathogenesis is not well understood and small animal models for the disease are lacking. Recently, the chromosomal translocation t(5;9)(q33;q22) generating the interleukin-2 (IL-2)-inducible T cell kinase (ITK)-spleen tyrosine kinase (SYK) fusion tyrosine kinase was identified as a recurrent event in PTCL. We show that ITK-SYK associates constitutively with lipid rafts in T cells and triggers antigen-independent phosphorylation of T cell receptor (TCR)-proximal proteins. These events lead to activation of downstream pathways and acute cellular outcomes that correspond to regular TCR ligation, including up-regulation of CD69 or production of IL-2 in vitro or deletion of thymocytes and activation of peripheral T cells in vivo. Ultimately, conditional expression of patient-derived ITK-SYK in mice induces highly malignant PTCLs with 100% penetrance that resemble the human disease. Our work demonstrates that constitutively enforced antigen receptor signaling can, in principle, act as a powerful oncogenic driver. Moreover, we establish a robust clinically relevant and genetically tractable model of human PTCL.
authors.affiliation: Third Medical Department, Technical University of Munich, Klinikum rechts der Isar, 81675 Munich, Germany.
authors.forename: Konstanze
authors.initials: K
authors.lastname: Pechloff
authors.forename: Julian
authors.initials: J
authors.lastname: Holch
authors.forename: Uta
authors.initials: U
authors.lastname: Ferch
authors.forename: Marc
authors.initials: M
authors.lastname: Schweneker
authors.forename: Kristina
authors.initials: K
authors.lastname: Brunner
authors.forename: Markus
authors.initials: M
authors.lastname: Kremer
authors.forename: Tim
authors.initials: T
authors.lastname: Sparwasser
authors.forename: Leticia
authors.initials: L
authors.lastname: Quintanilla-Martinez
authors.forename: Ursula
authors.initials: U
authors.lastname: Zimber-Strobl
authors.forename: Berthold
authors.initials: B
authors.lastname: Streubel
authors.forename: Andreas
authors.initials: A
authors.lastname: Gewies
authors.forename: Christian
authors.initials: C
authors.lastname: Peschel
authors.forename: Jürgen
authors.initials: J
authors.lastname: Ruland
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D004195:Disease Models, Animal; D053595:Embryonic Stem Cells / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D037181:Lectins, C-Type / Q000235:genetics; D016411:Lymphoma, T-Cell, Peripheral / Q000235:genetics* / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000235:genetics / Q000473:pathology; D051379:Mice; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D013154:Spleen / Q000201:enzymology; D000072377:Syk Kinase; D014178:Translocation, Genetic; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D007376:Interleukin-2; D047908:Intracellular Signaling Peptides and Proteins; D037181:Lectins, C-Type; D011505:Protein-Tyrosine Kinases; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins; D000072377:Syk Kinase; D000099192:CD69 Antigens; C000606896:SYK protein, human; C000606897:Syk protein, mouse; C080813:emt protein-tyrosine kinase