title: Note: Dynamic point spread function for single and multiphoton fluorescence microscopy.
abstract: We propose and demonstrate a dynamic point spread function (PSF) for single and multiphoton fluorescence microscopy. The goal is to generate a PSF whose shape and size can be maneuvered from highly localized to elongated one, thereby allowing shallow-to-depth excitation capability during active imaging. The PSF is obtained by utilizing specially designed spatial filter and dynamically altering the filter parameters. We predict potential applications in nanobioimaging and fluorescence microscopy.
authors.affiliation: Department of Instrumentation, Indian Institute of Science, Bangalore 560012, India. partha@isu.iisc.ernet.in
authors.forename: Partha Pratim
authors.initials: PP
authors.lastname: Mondal
authors.forename: Subhra
authors.initials: S
authors.lastname: Mandal
authors.forename: Alberto
authors.initials: A
authors.lastname: Diaspro
mesh_terms: D000465:Algorithms; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*