[Effect of hTERT antisense oligodeoxynucleotide on telomerase activity in bladder cancer cells in vitro].
OBJECTIVE
To investigate the effect of hTERT antisense oligodeoxynucleotide on telomerase activity in bladder cancer cells.


METHODS
Antisense phosphorothioate oligodeoxynucleotide (AS PS-ODN) was synthesized and purified. Telomerase activity was measured by telomerase PCR ELISA kit. hTERT mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR), and hTERT protein by immunohistochemistry and flow cytometry.


RESULTS
Telomerase activity was decreased in T24 cells 48 h after treatment with AS PS-ODN, and was significantly inhibited at 72 h.


CONCLUSIONS
AS PS-ODN can significantly inhibit telomerase activity by down-regulating hTERT mRNA and protein expression in bladder cancer cells.
Gao
Xiao-dong
XD
Department of Urology, Lanzhou General Hospital of Lanzhou Command, Lanzhou 730050, China. gxd58038@yahoo.com.cn
Chen
Yi-rong
YR
D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000201:enzymology* / Q000473:pathology
D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; C509186:TERT protein, human; D019098:Telomerase