Cell proliferation of HaCaT keratinocytes on collagen films modified by argon plasma treatment.
Argon plasma treatment was used to modify the surface of atelocollagen films using a plasmochemical reactor. To evaluate the effects of the treatment, the untreated and treated samples were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) imaging, and X-ray Photoelectron Spectroscopy (XPS) techniques. Cell growth was carried out by culturing human immortalized keratinocyte (HaCaT) cells and proliferation was measured via MTT assay. It was observed that argon plasma treatment significantly enhanced the extent of cell proliferation, which was ascribed to the favourable role of plasma treatment in inducing surface oxygen-containing entities together with increasing surface roughness. This can be considered as a potentially promising approach for tissue regeneration purposes.
García
Jorge López
JL
Polymer Centre, Faculty of Technology, Tomas Bata University in Zlín, T.G.M Sq. 275, Zlín, Czech Republic. vextropk@gmail.com
Asadinezhad
Ahmad
A
Pacherník
Jirí
J
Lehocký
Marián
M
Junkar
Ita
I
Humpolícek
Petr
P
Sáha
Petr
P
Valásek
Pavel
P
D001128:Argon / Q000737:chemistry*; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D056951:Photoelectron Spectroscopy; D012038:Regeneration; D017550:Spectroscopy, Fourier Transform Infrared
C059821:atelocollagen; D001128:Argon; D003094:Collagen