Structural allele-specific patterns adopted by epitopes in the MHC-I cleft and reconstruction of MHC:peptide complexes to cross-reactivity assessment.
The immune system is engaged in a constant antigenic surveillance through the Major Histocompatibility Complex (MHC) class I antigen presentation pathway. This is an efficient mechanism for detection of intracellular infections, especially viral ones. In this work we describe conformational patterns shared by epitopes presented by a given MHC allele and use these features to develop a docking approach that simulates the peptide loading into the MHC cleft. Our strategy, to construct in silico MHC:peptide complexes, was successfully tested by reproducing four different crystal structures of MHC-I molecules available at the Protein Data Bank (PDB). An in silico study of cross-reactivity potential was also performed between the wild-type complex HLA-A2-NS31073 and nine MHC:peptide complexes presenting alanine exchange peptides. This indicates that structural similarities among the complexes can give us important clues about cross reactivity. The approach used in this work allows the selection of epitopes with potential to induce cross-reactive immune responses, providing useful tools for studies in autoimmunity and to the development of more comprehensive vaccines.
Antunes
Dinler A
DA
Núcleo de Bioinformática do Laboratório de Imunogenética, Department of Genetics, Federal University of Rio Grande do Sul, Porto Alegre, Brasil.
Vieira
Gustavo F
GF
Rigo
Maurício M
MM
Cibulski
Samuel P
SP
Sinigaglia
Marialva
M
Chies
José A B
JA
D000483:Alleles; D017951:Antigen Presentation; D019295:Computational Biology / Q000379:methods; D003198:Computer Simulation; D003429:Cross Reactions / Q000276:immunology*; D030562:Databases, Protein; D000939:Epitopes / Q000737:chemistry*; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008285:Major Histocompatibility Complex; D008968:Molecular Conformation; D011485:Protein Binding
D000939:Epitopes; D015395:Histocompatibility Antigens Class I