title: Tissue depletion and concentration correlations between edible tissues and biological fluids of 3-amino-2-oxazolidinone in pigs fed with a furazolidone-medicated feed.
abstract: Furazolidone has been prohibited for use in food animal production worldwide for its carcinogenicity and mutagenicity, but it is still illegally used in some farms because of its effectiveness and cheap price. Because of the food safety and economical concerns, it is necessary to find an efficient and low-cost way to monitor the misuse of furazolidone in food-producing animals. For this regard, the tissue depletion and tissue-biological fluid concentration correlations of 3-amino-2-oxazolidinone (AOZ), which is the marker residue of furazolidone, were studied in pigs. Pigs were dosed with 400 mg/kg of furazolidone in feed for 7 days and were sacrificed at the withdrawal time of 0.5, 7, 21, 35, 56, and 63 days. Muscle, liver, kidney, urine, and plasma were collected to detect the AOZ by a simplified indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). Results showed that AOZ was widely distributed in pigs and eliminated slowly after the digestion of furazolidone. The half-lives of AOZ in the plasma, urine, liver, kidney, and muscle were 13.7, 14.7, 13.6, 13.6, and 15.0 days, respectively. Good correlations of the AOZ concentration were found between plasma and muscle, plasma and liver, urine and liver, and urine and kidney in the depletion period of 7-63 or 21-63 days, with correlation coefficients of more than 0.97 and p values less than 0.05. These correlations can provide a basis for a simple and economical way using plasma/urine to monitor the illegal use of furazolidone in pigs without slaughter.
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues HZAU)and MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan, Hubei 430070, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Lingli
authors.initials: L
authors.lastname: Huang
authors.forename: Yulian
authors.initials: Y
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Awais
authors.initials: A
authors.lastname: Ishan
authors.forename: Ke
authors.initials: K
authors.lastname: Fang
authors.forename: Dapeng
authors.initials: D
authors.lastname: Peng
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Liu
authors.forename: Menghong
authors.initials: M
authors.lastname: Dai
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000825:Animal Structures / Q000737:chemistry*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000493:pharmacokinetics*; D001826:Body Fluids / Q000737:chemistry*; D004350:Drug Residues / Q000032:analysis / Q000493:pharmacokinetics*; D008297:Male; D008460:Meat / Q000032:analysis; D023303:Oxazolidinones / Q000032:analysis / Q000493:pharmacokinetics*; D013552:Swine; D014018:Tissue Distribution; D019155:Veterinary Drugs / Q000032:analysis / Q000493:pharmacokinetics*
chemical_list: D000900:Anti-Bacterial Agents; D023303:Oxazolidinones; D019155:Veterinary Drugs; C000209:3-amino-2-oxazolidinone