Characterization of distinct mesenchymal-like cell populations from human skeletal muscle in situ and in vitro.
Human skeletal muscle is an essential source of various cellular progenitors with potential therapeutic perspectives. We first used extracellular markers to identify in situ the main cell types located in a satellite position or in the endomysium of the skeletal muscle. Immunohistology revealed labeling of cells by markers of mesenchymal (CD13, CD29, CD44, CD47, CD49, CD62, CD73, CD90, CD105, CD146, and CD15 in this study), myogenic (CD56), angiogenic (CD31, CD34, CD106, CD146), hematopoietic (CD10, CD15, CD34) lineages. We then analysed cell phenotypes and fates in short- and long-term cultures of dissociated muscle biopsies in a proliferation medium favouring the expansion of myogenic cells. While CD56(+) cells grew rapidly, a population of CD15(+) cells emerged, partly from CD56(+) cells, and became individualized. Both populations expressed mesenchymal markers similar to that harboured by human bone marrow-derived mesenchymal stem cells. In differentiation media, both CD56(+) and CD15(+) cells shared osteogenic and chondrogenic abilities, while CD56(+) cells presented a myogenic capacity and CD15(+) cells presented an adipogenic capacity. An important proportion of cells expressed the CD34 antigen in situ and immediately after muscle dissociation. However, CD34 antigen did not persist in culture and this initial population gave rise to adipogenic cells. These results underline the diversity of human muscle cells, and the shared or restricted commitment abilities of the main lineages under defined conditions.
Lecourt
Séverine
S
UPMC/AIM UMR S 974, Groupe Hospitalier Pitié-Salpêtrière, Paris, France. severine.lecourt@sls.aphp.fr
Marolleau
Jean-Pierre
JP
Fromigué
Olivia
O
Vauchez
Karine
K
Andriamanalijaona
Rina
R
Ternaux
Brigitte
B
Lacassagne
Marie-Noëlle
MN
Robert
Isabelle
I
Boumédiene
Karim
K
Chéreau
Frédéric
F
Marie
Pierre
P
Larghéro
Jérôme
J
Fiszman
Marc
M
Vilquin
Jean-Thomas
JT
D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002999:Clone Cells; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D008280:Magnetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D008863:Microspheres; D018482:Muscle, Skeletal / Q000166:cytology* / Q000276:immunology / Q000378:metabolism / Q000502:physiology
D015415:Biomarkers