title: Ammonia fiber expansion (AFEX) pretreatment, enzymatic hydrolysis, and fermentation on empty palm fruit bunch fiber (EPFBF) for cellulosic ethanol production.
abstract: Empty palm fruit bunch fiber (EPFBF), a readily available cellulosic biomass from palm processing facilities, is investigated as a potential carbohydrate source for cellulosic ethanol production. This feedstock was pretreated using ammonia fiber expansion (AFEX) and enzymatically hydrolyzed. The best tested AFEX conditions were at 135 °C, 45 min retention time, water to dry biomass loading of 1:1 (weight ratio), and ammonia to dry biomass loading of 1:1 (weight ratio). The particle size of the pretreated biomass was reduced post-AFEX. The optimized enzyme formulation consists of Accellerase (84 μL/g biomass), Multifect Xylanase (31 μL/g biomass), and Multifect Pectinase (24 μL/g biomass). This mixture achieved close to 90% of the total maximum yield within 72 h of enzymatic hydrolysis. Fermentation on the water extract of this biomass affirms that nutrients solely from the pretreated EPFBF can support yeast growth for complete glucose fermentation. These results suggest that AFEX-treated EPFBF can be used for cellulosic biofuels production because biomass recalcitrance has been overcome without reducing the fermentability of the pretreated materials.
authors.affiliation: Department of Chemical Engineering and Materials Science, Michigan State University, Lansing, 48910, USA.
authors.forename: Ming J
authors.initials: MJ
authors.lastname: Lau
authors.forename: Ming W
authors.initials: MW
authors.lastname: Lau
authors.forename: Christa
authors.initials: C
authors.lastname: Gunawan
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Dale
mesh_terms: D000641:Ammonia / Q000737:chemistry; D028023:Arecaceae / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005638:Fruit / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D006868:Hydrolysis; D007220:Industrial Waste / Q000032:analysis; D011096:Polygalacturonase / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D007220:Industrial Waste; D000431:Ethanol; D000641:Ammonia; D002482:Cellulose; D011096:Polygalacturonase; D043364:Endo-1,4-beta Xylanases