MALE GAMETOPHYTE DEFECTIVE 1, encoding the FAd subunit of mitochondrial F1F0-ATP synthase, is essential for pollen formation in Arabidopsis thaliana.
In flowering plants, pollen formation is a complex process and strictly controlled by genetic factors. Although thousands of genes have been identified to be highly or specifically expressed in pollen grains, little is known about the functions and regulatory mechanisms of the genes in pollen formation. Here we report the characterization of a novel gene, MALE GAMETOPHYTE DEFECTIVE 1 (MGP1), that is essential for pollen formation in Arabidopsis thaliana. MGP1 encodes the F(A)d subunit of mitochondrial F(1)F(0)-ATP synthase in Arabidopsis. It was highly expressed in pollen grains at the later developmental stage. Mutation in MGP1 led to destruction of the mitochondria in pollen grains at the dehydration stage and subsequently death of the pollen grains. These results suggested that MGP1 plays important roles in pollen formation, possibly by regulating the activity of mitochondrial F(1)F(0)-ATP synthase in Arabidopsis pollen grains.
Li
Wen-Qing
WQ
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Zhang
Xue-Qin
XQ
Xia
Chuan
C
Deng
Yi
Y
Ye
De
D
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000201:enzymology / Q000648:ultrastructure; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D011058:Pollen / Q000235:genetics / Q000254:growth & development*; D016415:Sequence Alignment
D029681:Arabidopsis Proteins; C474451:F1F0-ATP synthase; D025261:Mitochondrial Proton-Translocating ATPases