title: Assessment of CD8(+) T cell differentiation in Trypanosoma cruzi-infected children.
abstract: We previously reported that the T cell compartment in chronically Trypanosoma cruzi-infected adult subjects display functional and phenotypic signs of immune senescence. This study aimed to investigate the differentiation and the senescent profile of the overall CD8(+) T cell compartment in T. cruzi-infected children at the early stage of the disease. We found a lower percentage of naive (CD27(+)CD28(+)CD45RA(+)) and early antigen-experienced (CD45RA(-)CD27(+)CD28(+)), and higher percentages of late differentiated antigen-experienced (CD45RA(-)CD27(-)CD28(-)) CD8(+) T cells in T. cruzi-infected children as compared with age-matched uninfected controls. The expression of the interleukin (IL)-7R is also decreased on naive and on antigen-experienced total CD8(+) T cells with various degrees of differentiation. Conversely, the expression of HLA-DR, caspase-3, and CD57 did not vary on the total CD8(+) T cell compartment. These findings suggest that the duration of the infection is relevant in the process of immune senescent that this parasite can induce.
authors.affiliation: Instituto Nacional de Parasitología "Dr. M. Fatala Chaben," Ciudad Autónoma de Buenos Aires, Argentina. mcalbareda@gmail.com
authors.forename: María Cecilia
authors.initials: MC
authors.lastname: Albareda
authors.forename: Gabriela Carina
authors.initials: GC
authors.lastname: Olivera
authors.forename: Ana María
authors.initials: AM
authors.lastname: De Rissio
authors.forename: Miriam
authors.initials: M
authors.lastname: Postan
mesh_terms: D000293:Adolescent; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology*; D016022:Case-Control Studies; D002454:Cell Differentiation; D014355:Chagas Disease / Q000276:immunology*; D002648:Child; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D014349:Trypanosoma cruzi / Q000276:immunology
chemical_list: D015703:Antigens, CD; D016207:Cytokines