title: Characterization of distinct mesenchymal-like cell populations from human skeletal muscle in situ and in vitro.
abstract: Human skeletal muscle is an essential source of various cellular progenitors with potential therapeutic perspectives. We first used extracellular markers to identify in situ the main cell types located in a satellite position or in the endomysium of the skeletal muscle. Immunohistology revealed labeling of cells by markers of mesenchymal (CD13, CD29, CD44, CD47, CD49, CD62, CD73, CD90, CD105, CD146, and CD15 in this study), myogenic (CD56), angiogenic (CD31, CD34, CD106, CD146), hematopoietic (CD10, CD15, CD34) lineages. We then analysed cell phenotypes and fates in short- and long-term cultures of dissociated muscle biopsies in a proliferation medium favouring the expansion of myogenic cells. While CD56(+) cells grew rapidly, a population of CD15(+) cells emerged, partly from CD56(+) cells, and became individualized. Both populations expressed mesenchymal markers similar to that harboured by human bone marrow-derived mesenchymal stem cells. In differentiation media, both CD56(+) and CD15(+) cells shared osteogenic and chondrogenic abilities, while CD56(+) cells presented a myogenic capacity and CD15(+) cells presented an adipogenic capacity. An important proportion of cells expressed the CD34 antigen in situ and immediately after muscle dissociation. However, CD34 antigen did not persist in culture and this initial population gave rise to adipogenic cells. These results underline the diversity of human muscle cells, and the shared or restricted commitment abilities of the main lineages under defined conditions.
authors.affiliation: UPMC/AIM UMR S 974, Groupe Hospitalier Pitié-Salpêtrière, Paris, France. severine.lecourt@sls.aphp.fr
authors.forename: Séverine
authors.initials: S
authors.lastname: Lecourt
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Marolleau
authors.forename: Olivia
authors.initials: O
authors.lastname: Fromigué
authors.forename: Karine
authors.initials: K
authors.lastname: Vauchez
authors.forename: Rina
authors.initials: R
authors.lastname: Andriamanalijaona
authors.forename: Brigitte
authors.initials: B
authors.lastname: Ternaux
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Lacassagne
authors.forename: Isabelle
authors.initials: I
authors.lastname: Robert
authors.forename: Karim
authors.initials: K
authors.lastname: Boumédiene
authors.forename: Frédéric
authors.initials: F
authors.lastname: Chéreau
authors.forename: Pierre
authors.initials: P
authors.lastname: Marie
authors.forename: Jérôme
authors.initials: J
authors.lastname: Larghéro
authors.forename: Marc
authors.initials: M
authors.lastname: Fiszman
authors.forename: Jean-Thomas
authors.initials: JT
authors.lastname: Vilquin
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002999:Clone Cells; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D008280:Magnetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D008863:Microspheres; D018482:Muscle, Skeletal / Q000166:cytology* / Q000276:immunology / Q000378:metabolism / Q000502:physiology
chemical_list: D015415:Biomarkers