title: Changes in mitochondrial respiratory enzyme activity after ischemia-reperfusion injury in living-donor liver transplantation.
abstract: BACKGROUND
  Ischemia-reperfusion (I-R) injury plays an important role in the immediate graft function in living-donor liver transplantation (LDLT). There is growing evidence that mitochondria play a pivotal role in I-R injury. Our aim was to evaluate changes in mitochondrial respiratory enzyme activities after I-R injury in LDLT.
  
  
  METHODS
  Specimens from 8 donor recipient pairs enrolled in this study were obtained from the donor livers before harvest (before I-R injury) and after vascular anastomosis in the recipient (after I-R injury). Histidine-tryptophan-ketoglutarate solution was used to perfuse the organ during the cold ischemic period between harvesting and transplantation. We correlated changes in mitochondrial respiratory enzyme complex activity (succinate cytochrome c reductase [SCCR]; NADH cytochrome c reductase [NCCR]) after I-R injury with clinical data and graft status.
  
  
  RESULTS
  NCCR and SCCR activities did not uniformly decrease after I-R injury. Two of 8 recipients experienced graft dysfunction after transplantation. The decrease in neither NCCR nor SCCR activity correlated with graft dysfunction in these 2 patients. Among the clinical factors, grafts from older donors tended to show decreased NCCR activity after I-R injury.
  
  
  CONCLUSIONS
  In this study, changes in mitochondrial respiratory enzyme activity failed to predict the severity of I-R injury in LDLT. The organ preservation solution may play a protective role on mitochondrial respiratory enzymes during I-R injury.
authors.affiliation: Division of Transplantation Surgery, Department of Surgery, Taipei Veterans General Hospital, National Yang-Ming University School of Medicine, Taipei, Taiwan.
authors.forename: N-C
authors.initials: NC
authors.lastname: Lin
authors.forename: C-S
authors.initials: CS
authors.lastname: Liu
authors.forename: C-J
authors.initials: CJ
authors.lastname: Chang
authors.forename: C-C
authors.initials: CC
authors.lastname: Loong
authors.forename: C-Y
authors.initials: CY
authors.lastname: Hsia
authors.forename: H-L
authors.initials: HL
authors.lastname: Tsai
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D015415:Biomarkers; D005260:Female; D006801:Humans; D007700:Kinetics; D016031:Liver Transplantation / Q000009:adverse effects*; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D008930:Mitochondria, Liver / Q000201:enzymology*; D009245:NADH Dehydrogenase / Q000378:metabolism*; D015427:Reperfusion Injury / Q000201:enzymology*; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism*; D017211:Treatment Failure; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D013384:Succinate Cytochrome c Oxidoreductase; D009245:NADH Dehydrogenase