Effect of ketogenic diet on nucleotide hydrolysis and hepatic enzymes in blood serum of rats in a lithium-pilocarpine-induced status epilepticus.
The ketogenic diet (KD) is a high-fat and low-carbohydrate diet, used for treating refractory epilepsy in children. We have previously shown alterations in nucleotidase activities from the central nervous system and blood serum of rats submitted to different models of epilepsy. In this study we investigated the effect of KD on nucleotidase activities in the blood serum, as well if KD has any influence in the activity of liver enzymes such as alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase activities in Wistar rats submitted to the lithium-pilocarpine model of epilepsy. At 21 days of age, rats received an injection of lithium chloride and, 18-19 h later, they received an injection of pilocarpine hydrochloride for status epilepticus induction. The results reported herein show that seizures induced by lithium-pilocarpine elicit a significant increase in ATP hydrolysis and alkaline phosphatase activity, as well as a decrease in ADP hydrolysis and aspartate aminotransferase activity. The KD is a rigorous regimen that can be associated with hepatic damage, as shown herein by the elevated activities of liver enzymes and 5'-nucleotidase in blood serum. Further studies are necessary to investigate the mechanism of inhibition of lithium on nucleotidases in blood serum.
da Silveira
Vanessa Gass
VG
Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Anexo, Porto Alegre, Brazil.
de Paula Cognato
Giana
G
Müller
Alexandre Pastoris
AP
Figueiró
Fabrício
F
Bonan
Carla Denise
CD
Perry
Marcos L Santos
ML
Battastini
Ana Maria Oliveira
AM
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D003292:Convulsants / Q000008:administration & dosage; D055423:Diet, Ketogenic; D004195:Disease Models, Animal; D004798:Enzymes / Q000097:blood*; D005260:Female; D006868:Hydrolysis / Q000187:drug effects; D008094:Lithium / Q000008:administration & dosage*; D008099:Liver / Q000201:enzymology*; D009711:Nucleotides / Q000378:metabolism*; D010862:Pilocarpine / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D013226:Status Epilepticus / Q000097:blood* / Q000139:chemically induced / Q000178:diet therapy*
D003292:Convulsants; D004798:Enzymes; D009711:Nucleotides; D010862:Pilocarpine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D008094:Lithium; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase