title: Practical method for extraction of PCR-quality DNA from environmental soil samples.
abstract: Methods for the extraction of PCR-quality DNA from environmental soil samples by using pairs of commercially available kits were evaluated. Coxiella burnetii DNA was detected in spiked soil samples at <1,000 genome equivalents per gram of soil and in 12 (16.4%) of 73 environmental soil samples.
authors.affiliation: Centers for Disease Control and Prevention, MS G-13, 1600 Clifton Rd., Atlanta, GA 30333, USA.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Fitzpatrick
authors.forename: Gilbert J
authors.initials: GJ
authors.lastname: Kersh
authors.forename: Robert F
authors.initials: RF
authors.lastname: Massung
mesh_terms: D016997:Coxiella burnetii / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic; D012987:Soil