Characterization of triterpenic acids in fruits of ziziphus species by HPLC-ELSD-MS.
The fruits of Ziziphus species have been utilized as food as well as crude drugs for their health benefits in China for thousands of years. This paper reported a reversed-phase high-performance liquid chromatography (HPLC) method for the simultaneous characterization and quantitation of 11 triterpenic acids in chloroform extracts of jujube fruits by using an evaporative light scattering detector (ELSD) and electrospray ionization-mass spectrometry (ESI-MS). The results showed that the contents of triterpenic acids in the fruits of Ziziphus jujuba var. spinosa were higher than those in the fruits of Z. jujuba, especially for the compound pomonic acid. Differences were also found among the different parts of Z. jujuba var. spinosa fruits with the sarcocarp having a higher amount of triterpenic acids than the seed and hard core.
Guo
Sheng
S
Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, People's Republic of China.
Duan
Jin-Ao
JA
Tang
Yu-Ping
YP
Yang
Nian-Yun
NY
Qian
Da-Wei
DW
Su
Shu-Lan
SL
Shang
Er-Xin
EX
D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D008027:Light; D010936:Plant Extracts / Q000737:chemistry; D012542:Scattering, Radiation; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014315:Triterpenes / Q000032:analysis*; D031957:Ziziphus / Q000737:chemistry*
D010936:Plant Extracts; D014315:Triterpenes