Direct microfabrication of topographical and chemical cues for the guided growth of neural cell networks on polyamidoamine hydrogels.
Cell patterning is an important tool for organizing cells in surfaces and to reproduce in a simple way the tissue hierarchy and complexity of pluri-cellular life. The control of cell growth, proliferation and differentiation on solid surfaces is consequently important for prosthetics, biosensors, cell-based arrays, stem cell therapy and cell-based drug discovery concepts. We present a new electron beam lithography method for the direct and simultaneous fabrication of sub-micron topographical and chemical patterns, on a biocompatible and biodegradable PAA hydrogel. The localized e-beam modification of a hydrogel surface makes the pattern able to adsorb proteins in contrast with the anti-fouling surface. By also exploiting the selective attachment, growth and differentiation of PC12 cells, we fabricated a neural network of single cells connected by neuritis extending along microchannels. E-beam microlithography on PAA hydrogels opens up the opportunity of producing multifunctional microdevices incorporating complex topographies, allowing precise control of the growth and organization of individual cells.
Dos Reis
Gabriel
G
European School of Molecular Medicine - SEMM, IFOM-IEO campus, Via Adamello, 16 20139 Milan, Italy.
Fenili
Fabio
F
Gianfelice
Antonella
A
Bongiorno
Gero
G
Marchesi
Davide
D
Scopelliti
Pasquale Emanuele
PE
Borgonovo
Antonio
A
Podestà
Alessandro
A
Indrieri
Marco
M
Ranucci
Elisabetta
E
Ferruti
Paolo
P
Lenardi
Cristina
C
Milani
Paolo
P
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D020100:Hydrogels; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D009415:Nerve Net; D009757:Nylons; D016716:PC12 Cells; D011485:Protein Binding; D051381:Rats
D020100:Hydrogels; D009757:Nylons