title: Deep-tissue imaging of intramolecular fluorescence resonance energy-transfer parameters.
abstract: We demonstrate the in vivo reconstruction of all fluorescence resonance energy transfer (FRET) parameters, including the nanometer donor-acceptor distance, in a mouse. The FRET chemical targets cancer cells, and on internalization, the acceptor is released, in lieu of a targeted anticancer drug in chemotherapy. Our method provides a new vehicle for studying disease by imaging FRET parameters in deep tissue.
authors.affiliation: School of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Gaind
authors.forename: Sumith
authors.initials: S
authors.lastname: Kularatne
authors.forename: Philip S
authors.initials: PS
authors.lastname: Low
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Webb
mesh_terms: D000818:Animals; D004867:Equipment Design; D005260:Female; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation; D051379:Mice; D008819:Mice, Nude; D012878:Skin Neoplasms / Q000378:metabolism*