Metabolic imaging in the anesthetized rat brain using hyperpolarized [1-13C] pyruvate and [1-13C] ethyl pyruvate.
Formulation, polarization, and dissolution conditions were developed to obtain a stable hyperpolarized solution of [1-(13)C]-ethyl pyruvate. A maximum tolerated concentration and injection rate were determined, and (13)C spectroscopic imaging was used to compare the uptake of hyperpolarized [1-(13)C]-ethyl pyruvate relative to hyperpolarized [1-(13)C]-pyruvate into anesthetized rat brain. Hyperpolarized [1-(13)C]-ethyl pyruvate and [1-(13)C]-pyruvate metabolic imaging in normal brain is demonstrated and quantified in this feasibility and range-finding study.
Hurd
Ralph E
RE
Global Applied Science Laboratory, GE Healthcare, Menlo Park, California 94025, USA. ralph.hurd@ge.com
Yen
Yi-Fen
YF
Mayer
Dirk
D
Chen
Albert
A
Wilson
David
D
Kohler
Susan
S
Bok
Robert
R
Vigneron
Daniel
D
Kurhanewicz
John
J
Tropp
James
J
Spielman
Daniel
D
Pfefferbaum
Adolf
A
D018681:Anesthetics, General / Q000008:administration & dosage*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002247:Carbon Isotopes / Q000493:pharmacokinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D011773:Pyruvates / Q000493:pharmacokinetics*; D019289:Pyruvic Acid / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
D018681:Anesthetics, General; D002247:Carbon Isotopes; D011773:Pyruvates; C046522:ethyl pyruvate; D019289:Pyruvic Acid