A bioorthogonal chemistry strategy for probing protein lipidation in live cells.
We report a chemical lipidation model for the study of protein lipidations in vitro and in live mammalian cells based on a bioorthogonal, photoinduced tetrazole-alkene cycloaddition reaction.
Song
Wenjiao
W
Department of Chemistry, State University of New York at Buffalo, Buffalo, New York 14260, USA.
Yu
Zhipeng
Z
Madden
Michael M
MM
Lin
Qing
Q
D004591:Electrophoresis, Polyacrylamide Gel; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D015394:Molecular Structure; D010777:Photochemistry / Q000379:methods; D011506:Proteins / Q000737:chemistry*; D013777:Tetrazoles / Q000737:chemistry*
D011506:Proteins; D013777:Tetrazoles; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C045574:1H-tetrazole