[Effect of JAK2/STAT3/vimentin signaling pathway on proliferation and migration of human colon cancer cells].
OBJECTIVE
To investigate the regulatory role of JAK2/STAT3/vimentin signaling pathway on the proliferation and migration of human colon cancer cells.


METHODS
The human colon cancer cell Lovo was treated with Janus kinase inhibitor AG490. The proliferation of Lovo cells was quantified by MTT assay. The migration of Lovo cells was measured with scratch assay. The intracellular phosphorylation STAT3 (P-STAT3) and vimentin protein was detected by Western blot and immunofluorescence.


RESULTS
After AG490 treatment, the proliferative ability of Lovo cells decreased as compared with control group (P<0.05). This suppression was dose-independent and time-independent. At 24 hrs after scratching, scratch width in the AG490 group recovered to 20%, lower than that in the control group (60%, P<0.05). After AG490 treatment, the expression of P-STAT3 and vimentin in Lovo cells decreased significantly (P<0.05).


CONCLUSIONS
JAK2/STAT3/vimentin signaling pathway participates in regulating the proliferation and migration of human colon cancer cells.
Xu
Jian-hua
JH
Department of General Surgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
Zhang
Chao
C
Tang
Bo
B
Hao
Ying-xue
YX
Chen
Jun
J
Liu
Tao
T
Cui
Hao
H
D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D053614:Janus Kinase 2 / Q000378:metabolism*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D014746:Vimentin / Q000378:metabolism*
D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D014746:Vimentin; C507924:JAK2 protein, human; D053614:Janus Kinase 2