Multiresidue method for the determination of nitroimidazoles and their hydroxy-metabolites in poultry muscle, plasma and egg by isotope dilution liquid chromatography-mass spectrometry.
A multiresidue analytical procedure for the determination of four nitroimidazoles (metronidazole, dimetridazole, ronidazole, ipronidazole) and their hydroxy-metabolites in poultry muscle, plasma and egg is presented. The procedure is based on ion-exchange solid phase extraction with acetonitrile as an extractant followed by liquid chromatography-mass spectrometry. The separation of analytes was performed on a C18 column using a mobile phase of 0.1% formic acid in acetonitrile and 0.1% formic acid in water with gradient elution. The electrospray ionization was used to obtain the protonated molecules [M+H](+) and two product ions were monitored for each compound. For the quantification stable isotope-labelled analogues of the analytes were used as internal standards. The whole procedure was evaluated according to EU Commission Decision 2002/657/EC requirements. Specificity, decision limit (CCalpha), detection capacity (CCbeta), recovery and precision were determined during validation process. The overall recoveries ranged between 93 and 103% with a good coefficient of variation, less than 14.0% under within-laboratory reproducibility conditions. CCalpha and CCbeta were 0.05-0.44 and 0.08-0.90microgkg(-1) depending on analyte and matrix.
Mitrowska
Kamila
K
National Veterinary Research Institute, Department of Pharmacology and Toxicology, Al. Partyzantow 57, Pulawy, Poland. kamitro@piwet.pulawy.pl
Posyniak
Andrzej
A
Zmudzki
Jan
J
D000818:Animals; D002138:Calibration; D002623:Chemistry Techniques, Analytical; D002853:Chromatography, Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis*; D005504:Food Analysis; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D009593:Nitroimidazoles / Q000032:analysis* / Q000737:chemistry*; D011200:Poultry; D015203:Reproducibility of Results; D013997:Time Factors
D009593:Nitroimidazoles