title: Relationship between polymorphonuclear leukocyte count in bronchoalveolar lavage fluid and bacterial content in Gram's stain and bacterial content in final microbiological report.
abstract: Eighty samples of bronchoalveolar lavage fluid (BALF) were obtained from the total of 48 patients (22 females and 26 males) and analyzed. Eighteen of those patients were organ transplant recipients. The relationship between polymorphonuclear leukocyte (PMN) count in direct sample and semi quantitative Gram-positive and Gram-negative bacterial content were analyzed in BALF samples. PMN count in direct sample and Gram-positive and Gram-negative bacterial content of the final microbiological report was compared as well. On the total number of samples PMN count in direct samples of BALF was statistically significant regarding the presence of Gram-positive bacteria in the same sample; it was nearly significant regarding the presence of Gram-negative bacteria; and it was statistically significant for the total bacterial content. If BALF samples are divided into those obtained from organ-transplant and those obtained from non-organ-transplant patients, positive, statistically significant relationship is found in the organ-transplant group, more specifically for the relationship between PMNs and total bacterial content. When PMN count in direct microbiological sample was compared with the results of the final microbiological report, statistically significant relationship was found neither with respect to all BALF samples, nor after dividing them into "organ-transplant" and "non-organ-transplant" group. We did not find differences caused by gender.
authors.affiliation: Department of Internal Medicine, University Hospital "Merkur", Zagreb, Croatia. gcavric@yahoo.com
authors.forename: Gordana
authors.initials: G
authors.lastname: Cavrić
authors.forename: Slavica Naumovski
authors.initials: SN
authors.lastname: Mihalić
authors.forename: Sanda Janković
authors.initials: SJ
authors.lastname: Tesanović
authors.forename: Matea Bogdanović
authors.initials: MB
authors.lastname: Dvorsćak
authors.forename: Gorjana
authors.initials: G
authors.lastname: Erceg
authors.forename: Marijana Rehorić
authors.initials: MR
authors.lastname: Krkusek
authors.forename: Dubravka
authors.initials: D
authors.lastname: Bartolek
authors.forename: Klara
authors.initials: K
authors.lastname: Jurić
authors.forename: Khaled
authors.initials: K
authors.lastname: Nassabain
authors.forename: Ivan
authors.initials: I
authors.lastname: Budimir
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000382:microbiology; D005260:Female; D005840:Gentian Violet; D006801:Humans; D016867:Immunocompromised Host / Q000276:immunology*; D007958:Leukocyte Count; D008172:Lung Diseases, Fungal / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology; D008297:Male; D008828:Microbiological Techniques; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology*; D016377:Organ Transplantation / Q000706:statistics & numerical data*; D010619:Phenazines; D018410:Pneumonia, Bacterial / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology; D012307:Risk Factors; D012727:Sex Characteristics; D017678:Sex Distribution; D055815:Young Adult
chemical_list: C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet