Synthesis and characterization of a molecularly imprinted polymer and its application as SPE enrichment sorbent for determination of trace methimazole in pig samples using HPLC-UV.
A novel molecularly imprinted polymer that could be applied as enrichment sorbent was prepared using methimazole (MMZ) as the template molecule, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker. Though evaluated by static, kinetic and competitive adsorption tests, the polymer exhibited high adsorption capacity, fast kinetics and good selective ability. A method for determination of trace MMZ was developed using this polymer as enrichment sorbent coupled with high performance liquid chromatography focusing on complex biological matrices. Under the optimum experimental conditions, the MMZ standard is linear within the concentration range studied, that is, from 0.5 microg L(-1) to 150 microg L(-1) (r2=0.9941). Lower limits of detection (LOD, at S/N=3) and quantification (LOQ, at S/N=10) in pig samples were 0.63 microg kg(-1) and 2.10 microg kg(-1) for kidney, 0.51 microg kg(-1) and 1.70 microg kg(-1) for liver, 0.56 microg kg(-1) and 1.86 microg kg(-1) for muscle, respectively. Recoveries and relative standard deviation (RSD, n=9) values for precision in the developed method were from 71.14% to 88.41% and from 2.53% to 6.18%.
Pan
Mingfei
M
Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin, 300457, China.
Wang
Junping
J
Fang
Guozhen
G
Tang
Weiwei
W
Wang
Shuo
S
D000327:Adsorption; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007668:Kidney / Q000737:chemistry; D007700:Kinetics; D016014:Linear Models; D008099:Liver / Q000737:chemistry; D008713:Methimazole / Q000032:analysis*; D054802:Molecular Imprinting; D009132:Muscles / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D013552:Swine
D011108:Polymers; D008713:Methimazole