title: Transcriptional and post-transcriptional response of drug-metabolizing enzymes to PAHs contamination in red mullet (Mullus barbatus, Linnaeus, 1758): a field study.
abstract: Aim of this study was to evaluate the responsiveness of red mullet (Mullus barbatus) liver detoxification enzymes to PAHs at transcriptional and post-transcriptional levels in the field. Fish were captured in the north-eastern Adriatic Sea, close to an oil refinery. Sixteen PAHs (EPA) were determined in sediments and fish fillets; transcription levels of cyp1a, cyp3a and abcc2 genes and EROD, BROD, B(a)PMO, BFCOD, GST and UDPGT enzymatic activities were measured. Levels of PAHs in sediments reflect the oil pollution gradient of the area, with weak correspondence in fish fillets. cyp1a gene transcription and EROD, B(a)PMO and BFCOD activities were significantly induced in the oil refinery site, and a slight up-regulation of cyp3a and abcc2 was also observed. GST and UDPGT remained unchanged. The present study provides the first data on detoxification responses at transcriptional levels in the liver of red mullet and confirms phase I enzymes as suitable biomarkers of exposure to PAHs in field studies.
authors.affiliation: Department of Environmental Sciences "G. Sarfatti", University of Siena, Via Mattioli 4, 53100 Siena, Italy. dellatorre2@unisi.it
authors.forename: Camilla
authors.initials: C
authors.lastname: Della Torre
authors.forename: Ilaria
authors.initials: I
authors.lastname: Corsi
authors.forename: Francesco
authors.initials: F
authors.lastname: Nardi
authors.forename: Guido
authors.initials: G
authors.lastname: Perra
authors.forename: Maria Paola
authors.initials: MP
authors.lastname: Tomasino
authors.forename: Silvano
authors.initials: S
authors.lastname: Focardi
mesh_terms: D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D019362:Cytochrome P-450 CYP2B1 / Q000235:genetics / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D019015:Geologic Sediments / Q000737:chemistry; D014453:Glucuronosyltransferase / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000235:genetics / Q000378:metabolism; D008658:Inactivation, Metabolic; D008099:Liver / Q000378:metabolism; D010473:Perciformes / Q000235:genetics / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D014158:Transcription, Genetic / Q000187:drug effects*; D015533:Transcriptional Activation / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*
chemical_list: D029941:Fish Proteins; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1; D014453:Glucuronosyltransferase; D005978:Glutathione