The role of protein kinase C in the opening of blood-brain barrier induced by electromagnetic pulse.
The aim of this study was to determine the role of protein kinase C signaling in electromagnetic pulse (EMP)-induced blood-brain barrier (BBB) permeability change in rats. The protein level of total PKC and two PKC isoforms (PKC-alpha, and PKC-beta II) were determined in brain cerebral cortex microvessels by Western blot after exposing rats to EMP at 200kV/m for 200 pulses with 1Hz repetition rate. It was found that the protein level of PKC and PKC-betaII (but not PKC-alpha) in cerebral cortex microvessels increased significantly at 0.5h and 1h after EMP exposure compared with sham-exposed animals and then recovered at 3h. A specific PKC antagonist (H7) almost blocked EMP-induced BBB permeability change. EMP-induced BBB tight junction protein ZO-1 translocation was also inhibited. Our data indicated that PKC signaling was involved in EMP-induced BBB permeability change and ZO-1 translocation in rat.
Qiu
Lian-Bo
LB
Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an, China.
Ding
Gui-Rong
GR
Li
Kang-Chu
KC
Wang
Xiao-Wu
XW
Zhou
Yan
Y
Zhou
Yong-Chun
YC
Li
Yu-Rong
YR
Guo
Guo-Zhen
GZ
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D002540:Cerebral Cortex / Q000378:metabolism; D004574:Electromagnetic Fields; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D055806:Microvessels / Q000378:metabolism; D010539:Permeability / Q000187:drug effects / Q000528:radiation effects; D010750:Phosphoproteins / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D064546:Protein Kinase C beta; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D062826:Zonula Occludens-1 Protein
D008565:Membrane Proteins; D010750:Phosphoproteins; D047428:Protein Kinase Inhibitors; C569044:Tjp1 protein, rat; D062826:Zonula Occludens-1 Protein; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D064546:Protein Kinase C beta