Effect of the administration of Lactobacillus paracasei subsp. paracasei NTU 101 on Peyer's patch-mediated mucosal immunity.
The role of lactic acid bacteria in gut mucosal immunity was investigated by comparing the enhanced effects in the Peyer's patches and spleen of BALB/c mice fed daily with Lactobacillus paracasei subsp. paracasei NTU 101 for 3 to 9 weeks. After feeding with Lactobacillus, the percentage of CD4+ T cells in both Peyer's patches and the spleen was significantly increased; however, expression of CD 154 molecules, which play a pivotal role in cell-to-cell communication, on CD4+ T cells and the percentage of B220+ B cells increased only in Peyer's patches. Compared with systemic serum IgA, Peyer's patch-derived immunomodulation induced higher levels of intestinal IgA+-producing cells in the lamina propria. Our data also showed that feeding with Lactobacillus induced stronger CD4+ T cell-dendritic cell interaction, enhanced CD4+ T cell and B cell proliferation, and increased IL-1beta, IL-10, IL-12, IFN-gamma, and TNF-alpha mRNA expression in Peyer's patches, but not in the spleen. Here, we demonstrate that following Lactobacillus treatment, Peyer's patches exhibited a more distinct capacity to induce CD4+ T cell-dendritic cell interactions, lymphocyte proliferation, and cytokine secretion than the spleen, and thereby promoted greater intestinal IgA production that could enhance immunosurveillance to prevent intestinal infections or other intestinal pathologies.
Tsai
Yueh-Ting
YT
Institute of Microbiology and Biochemistry, College of Life Science, National Taiwan University, Taipei 10617, Taiwan.
Cheng
Po-Ching
PC
Liao
Jiunn-Wang
JW
Pan
Tzu-Ming
TM
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D023201:CD40 Ligand / Q000096:biosynthesis; D002450:Cell Communication; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D007778:Lactobacillus / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D010581:Peyer's Patches / Q000276:immunology / Q000382:microbiology / Q000473:pathology
D016207:Cytokines; D007070:Immunoglobulin A; D023201:CD40 Ligand; D017493:Leukocyte Common Antigens