Identification of acupuncture-specific proteins in the process of electro-acupuncture after spinal cord injury.
Spinal cord injury (SCI) is a serious condition often affecting young and healthy individuals around the world. Electro-acupuncture (EA) has been proven to contribute towards neurologic and functional recoveries in SCI, but the underlying mechanism remains largely unknown especially regarding neural specific proteins involved in the development of EA. The protein expression profile of spinal cord in both SCI and EA treatment models was analyzed by using two-dimensional electrophoresis-based proteomics. Using a MALDI-TOF/TOF MS and subsequent protein database searching, we identified changes in 15 proteins in the spinal cord following Governor Vessel (GV) EA treatment on SCI. These proteins are involved in inflammation, cell adhesion and migration, signal transduction and apoptosis processes. We selected 2 proteins (ANXA5 and CRMP2) beneficial to neuronal survival and axonal regeneration, and further identified these protein changes using Western blot analysis. Subsequently, Nissl staining and immunofluorescence double labeling approaches were used to explore possible role of the two neural specific proteins in the process of GV-EA treatment on SCI. Our results suggest that ANXA5 and CRMP2 may be neural specific proteins in the process of GV-EA treatment on SCI. This work might contribute to the better understanding of the mechanism involved in EA treatment on SCI at protein levels and provide a new therapeutic strategy for SCI.
Li
Wen-Jie
WJ
Division of Neuroscience, Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Pan
San-Qiang
SQ
Zeng
Yuan-Shan
YS
Su
Bao-Gui
BG
Li
Shu-Min
SM
Ding
Ying
Y
Li
Yan
Y
Ruan
Jing-Wen
JW
D000818:Animals; D017304:Annexin A5 / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology; D019771:Axotomy; D004195:Disease Models, Animal; D015671:Electroacupuncture / Q000379:methods*; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins; D009416:Nerve Regeneration / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D049488:Phosphatidylethanolamine Binding Protein / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013116:Spinal Cord / Q000737:chemistry / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000628:therapy*
D017304:Annexin A5; D055551:HSP27 Heat-Shock Proteins; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C550693:PEBP1 protein, rat; D049488:Phosphatidylethanolamine Binding Protein; C095114:collapsin response mediator protein-2