19F MRI of 3D CEM cells to study the effects of tocopherols and tocotrienols.
Oximetry of the human T-Lymphoblastoid (CEM) cells was measured using (19)F magnetic resonance imaging ((19)F MRI). The cells were treated with the analogues of vitamin E, alpha-, gamma-, delta-tocopherols and corresponding tocotrienols, ex vivo in three-dimensional (3D) cell culture. The study showed that (19)F MRI allows to measure the effect of the analogues due to changes of oxygenation, which were detected using MRI. Hexafluorobenzene was used as a (19)F MRI probe sensitive to oxygen concentrations. After 72h of treatment in HFBR with alpha-, gamma-, delta-tocopherols the oxygen concentration was 19.9+/-0.8%, 19.3+/-1.4%, 16+/-3.5%, respectively. The oxygen concentration in cells treated with alpha-, gamma-, delta-tocotrienols was found to be 14+/-1.5%, 10+/-1.2% and 8.8+/-1.1%, respectively whereas for the control cells it was 22.1+/-1%. The results show that delta-tocopherol and delta-tocotrienol are the most effective treatments in CEM cells among all the tested analogues.
Bartusik
Dorota
D
National Research Council Canada, Institute for Biodiagnostics West, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada. dorota.bartusik@gmail.com
Tomanek
Boguslaw
B
Siluk
Danuta
D
Kaliszan
Roman
R
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005462:Fluorine Radioisotopes / Q000032:analysis; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D013601:T-Lymphocytes / Q000187:drug effects*; D024505:Tocopherols / Q000494:pharmacology*; D024508:Tocotrienols / Q000494:pharmacology*
D005462:Fluorine Radioisotopes; D024508:Tocotrienols; D024505:Tocopherols