Expression of thermostable bacterial beta-glucosidase (BglB) in transgenic tobacco plants.
Transgenic tobacco plants expressing the hyperthermostable beta-glucosidase BglB of Thermotoga maritima were generated with the goal of cost-effective production of the enzyme for the application in bioconversion of lignocellulosic biomass. The enzyme was targeted to the cytosol and chloroplasts, where it accumulated to level of 4.5% and 5.8% of total soluble protein, respectively. The optimal temperature and pH of the plant-expressed BglB was 80 degrees C and 4.5, respectively. BglB activity was preserved in leaves after lyophilization, but decreased by over 70% with drying at room temperature. When BglB was synergistically supplied in a 1% (w/v) rice straw with Cel5A for efficient cellulase conversion, a 37% increase in glucose was observed. This report demonstrates the potential of utilizing transgenic tobacco for mass production of BglB.
Jung
Sera
S
Department of Forest Products and Technology (BK21 Program), Chonnam National University, Gwangju 500-757, Republic of Korea.
Kim
Suyeon
S
Bae
Hanhong
H
Lim
Hyoun-Sub
HS
Bae
Hyeun-Jong
HJ
D004789:Enzyme Activation; D004795:Enzyme Stability; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism*; D013696:Temperature; D020124:Thermotoga maritima / Q000201:enzymology*; D001617:beta-Glucosidase / Q000737:chemistry* / Q000378:metabolism*
D011994:Recombinant Proteins; D001617:beta-Glucosidase