Coop functions as a corepressor of Pangolin and antagonizes Wingless signaling.
Wingless (Wg) signaling regulates expression of its target genes via Pangolin and Armadillo, and their interacting cofactors. In the absence of Wg, Pangolin mediates transcriptional repression. In the presence of Wg, Pangolin, Armadillo, and a cohort of coactivators mediate transcriptional activation. Here we uncover Coop (corepressor of Pan) as a Pangolin-interacting protein. Coop and Pangolin form a complex on DNA containing a Pangolin/TCF-binding motif. Overexpression of Coop specifically represses Wg target genes, while loss of Coop function causes derepression. Finally, we show that Coop antagonizes the binding of Armadillo to Pangolin, providing a mechanism for Coop-mediated repression of Wg target gene transcription.
Song
Haiyun
H
Institute of Molecular Life Sciences, University of Zurich, CH-8057 Zurich, Switzerland.
Goetze
Sandra
S
Bischof
Johannes
J
Spichiger-Haeusermann
Chloe
C
Kuster
Marco
M
Brunner
Erich
E
Basler
Konrad
K
D000818:Animals; D051186:Armadillo Domain Proteins / Q000378:metabolism; D056970:Co-Repressor Proteins / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism; D051155:Wnt1 Protein / Q000378:metabolism*
C495305:ARM protein, Drosophila; D051186:Armadillo Domain Proteins; D056970:Co-Repressor Proteins; C549401:Coop protein, Drosophila; D029721:Drosophila Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D051155:Wnt1 Protein; C115159:pan protein, Drosophila; C074622:wg protein, Drosophila