title: The Scottish Structural Proteomics Facility: targets, methods and outputs.
abstract: The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology.
authors.affiliation: Biomedical Sciences Research Complex, University of St Andrews, St Andrews, KY16 9ST, UK.
authors.forename: Muse
authors.initials: M
authors.lastname: Oke
authors.forename: Lester G
authors.initials: LG
authors.lastname: Carter
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Johnson
authors.forename: Huanting
authors.initials: H
authors.lastname: Liu
authors.forename: Stephen A
authors.initials: SA
authors.lastname: McMahon
authors.forename: Xuan
authors.initials: X
authors.lastname: Yan
authors.forename: Melina
authors.initials: M
authors.lastname: Kerou
authors.forename: Nadine D
authors.initials: ND
authors.lastname: Weikart
authors.forename: Nadia
authors.initials: N
authors.lastname: Kadi
authors.forename: Md Arif
authors.initials: MA
authors.lastname: Sheikh
authors.forename: Stefan
authors.initials: S
authors.lastname: Schmelz
authors.forename: Mark
authors.initials: M
authors.lastname: Dorward
authors.forename: Michal
authors.initials: M
authors.lastname: Zawadzki
authors.forename: Christopher
authors.initials: C
authors.lastname: Cozens
authors.forename: Helen
authors.initials: H
authors.lastname: Falconer
authors.forename: Helen
authors.initials: H
authors.lastname: Powers
authors.forename: Ian M
authors.initials: IM
authors.lastname: Overton
authors.forename: C A Johannes
authors.initials: CA
authors.lastname: van Niekerk
authors.forename: Xu
authors.initials: X
authors.lastname: Peng
authors.forename: Prakash
authors.initials: P
authors.lastname: Patel
authors.forename: Roger A
authors.initials: RA
authors.lastname: Garrett
authors.forename: David
authors.initials: D
authors.lastname: Prangishvili
authors.forename: Catherine H
authors.initials: CH
authors.lastname: Botting
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Coote
authors.forename: David T F
authors.initials: DT
authors.lastname: Dryden
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Barton
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schwarz-Linek
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Challis
authors.forename: Garry L
authors.initials: GL
authors.lastname: Taylor
authors.forename: Malcolm F
authors.initials: MF
authors.lastname: White
authors.forename: James H
authors.initials: JH
authors.lastname: Naismith
mesh_terms: D019295:Computational Biology; D003460:Crystallization; D006801:Humans; D007753:Laboratories / Q000458:organization & administration*; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000458:organization & administration*; D012606:Scotland
chemical_list: D011506:Proteins