title: Direct acylation of carrier proteins with functionalized beta-lactones.
abstract: As the key component of many biosynthetic assemblies, acyl-carrier proteins offer a robust entry point for introduction of small molecule probes and pathway intermediates. Current labeling strategies primarily rely on modifications to the phosphopantetheine cofactor or its biosynthetic precursors followed by attachment to the apo form of a given carrier protein. As a greatly simplified alternative, direct and selective acylation of holo-acyl-carrier proteins using readily accessible beta-lactones as electrophilic partners for the phosphopantetheine-thiol has been demonstrated.
authors.affiliation: Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, Massachusetts 01003, USA.
authors.forename: Jon W
authors.initials: JW
authors.lastname: Amoroso
authors.forename: Lawrence S
authors.initials: LS
authors.lastname: Borketey
authors.forename: Gitanjeli
authors.initials: G
authors.lastname: Prasad
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Schnarr
mesh_terms: D002352:Carrier Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D007783:Lactones / Q000737:chemistry*; D015394:Molecular Structure
chemical_list: D002352:Carrier Proteins; D007783:Lactones