Identification of cells expressing cyclooxygenase-2 in response to interleukin-1beta in rat aortae.
Localization of cyclooxygenase-2 (COX-2) in rat aortae was investigated. In endothelium-intact aortic strips stimulated with interleukin-1beta (IL-1beta), COX-2 was detected in adventitial fibroblasts and macrophages but not in tunica media. IL-1beta-induced COX-2 expression was also detected in medial smooth muscle cells of endothelium-denuded aortae and cultured rat aortic smooth muscle cells. IL-1beta-induced COX-2 expression in all of the above cells was abolished in the presence of U0126, an inhibitor of extracellular signal-regulated kinases (ERK). Thus, IL-1beta induces COX-2 through the ERK-mediated pathway in adventitial fibroblasts and macrophages of healthy aortae; and in addition, medial smooth muscle cells are also responsible for COX-2 expression in remodeled aortae.
Nakano
Tomoyuki
T
Department of Environmental and Preventive Medicine, Hyogo College of Medicine, Japan.
Wakabayashi
Ichiro
I
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism*; D002070:Butadienes / Q000494:pharmacology; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000494:pharmacology; D066298:In Vitro Techniques; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D002070:Butadienes; D004791:Enzyme Inhibitors; D053583:Interleukin-1beta; D009570:Nitriles; C113580:U 0126; D051546:Cyclooxygenase 2