Development of a label free IGE sensitive aptasensor based on electrochemical impedance spectrometry.
A sensitive aptamer based electrochemical biosensor to detect human immunoglobulin E (IgE) is presented in this study. 5' Biotin labeled 45 mer DNA aptamer sequence was immobilized onto streptavidin coated graphite surfaces. Interaction between human IgE and DNA aptamer was monitored by Electrochemical Impedance Spectrometry (EIS) in a 0.48nM detection limit of IgE. EIS analysis are based on electron transfer resistance (Rct) in the presence of 5mM [Fe(CN)6]3-/4-.
Kara
Pinar
P
Department of Analytical Chemistry, Ege University, Bornova, Izmir, Turkey. pinar.kara@ege.edu.tr
Meric
Buket
B
Ozsoz
Mehmet
M
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017097:Electric Impedance; D004563:Electrochemistry; D006108:Graphite / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties
D052157:Aptamers, Nucleotide; D020099:Coated Materials, Biocompatible; D007073:Immunoglobulin E; D006108:Graphite; D019809:Streptavidin