Detection of feline panleukopenia virus using a commercial ELISA for canine parvovirus.
Feline panleukopenia virus (FPV) is a significant pathogen of cats. Rapid virus detection is critical for treatment and management, especially in populations in which spread may occur. This study investigated the ability of the SNAP Canine Parvovirus Antigen Test Kit (SNAP Parvo, IDEXX Laboratories) to detect FPV with confirmation of viral identity by polymerase chain reaction (PCR) assay and genetic sequencing on fecal samples (n = 97) from cats with suspected FPV infection. Fifty-five samples were positive by SNAP Parvo; 54 of 55 were also positive by conventional PCR assay and were identified as FPV by genetic sequencing. This study demonstrates that SNAP Parvo can detect FPV in clinical samples.
Abd-Eldaim
Mohamed
M
Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Knoxvill, TN 37996, USA.
Beall
Melissa J
MJ
Kennedy
Melissa A
MA
D000818:Animals; D002415:Cats; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005243:Feces / Q000821:virology; D005254:Feline Panleukopenia / Q000175:diagnosis / Q000821:virology*; D028301:Feline Panleukopenia Virus / Q000235:genetics / Q000302:isolation & purification*; D017992:Parvovirus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary