title: [Distraction osteogenesis for correction of cleft palate in rhesus-histological and fluorescent labeling study].
abstract: OBJECTIVE
  To investigate the mechanism of new bone formation in the distraction osteogenesis (DO) for correction of cleft palate (CP) in rhesus.
  
  
  METHODS
  CP was created by operation in 23 rhesus. The CP was corrected with DO in 21 animals as experimental group. The distraction rate was 0.8 mm per day, two times a day. The bone fragments were fixed after cleft closure, every 3 animals were sacrificed to get specimen after 1, 2, 4, 6, 8, 12, 24 weeks of fixation. 6 days before sacrifice, tetracycline was administrated for labeling (30 mg/kg).
  
  
  RESULTS
  The hard and soft tissue def of fixation. At the same time, the bone volume and calcification between the distraction gap increased. The cleft in the control group could not b ect was successfully closed with DO by intramembrane osteogenesis. The new formed bone was remodeling and became maturation during the period e corrected spontaneously.
  
  
  CONCLUSIONS
  The DO can successfully correct both the soft and hard tissue defect in CP by intramembrane osteogenesis. The fixation is important for remodeling and maturation of the new formed bone.
authors.affiliation: Department of Oral and Maxillofacial Surgery, the Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Yan-shan
authors.initials: YS
authors.lastname: Liu
authors.forename: Dai
authors.initials: D
authors.lastname: Shen
authors.forename: Tong
authors.initials: T
authors.lastname: Zhu
authors.forename: Zhi-qi
authors.initials: ZQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers; D002972:Cleft Palate / Q000473:pathology* / Q000601:surgery*; D008251:Macaca; D019857:Osteogenesis, Distraction; D021362:Palate, Hard / Q000473:pathology; D010160:Palate, Soft / Q000473:pathology
chemical_list: D015415:Biomarkers