title: Improving bacterial cellulose for blood vessel replacement: Functionalization with a chimeric protein containing a cellulose-binding module and an adhesion peptide.
abstract: Chimeric proteins containing a cellulose-binding module (CBM) and an adhesion peptide (RGD or GRGDY) were produced and used to improve the adhesion of human microvascular endothelial cells (HMEC) to bacterial cellulose (BC). The effect of these proteins on the HMEC-BC interaction was studied. The results obtained demonstrated that recombinant proteins containing adhesion sequences were able to significantly increase the attachment of HMEC to BC surfaces, especially the RGD sequence. The images obtained by scanning electron microscopy showed that the cells on the RGD-treated BC present a more elongated morphology 48h after cell seeding. The results also showed that RGD decreased the in-growth of HMEC cells through the BC and stimulated the early formation of cord-like structures by these endothelial cells. Thus, the use of recombinant proteins containing a CBM domain, with high affinity and specificity for cellulose surfaces allows control of the interaction of this material with cells. CBM may be combined with virtually any biologically active protein for the modification of cellulose-based materials, for in vitro or in vivo applications.
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal.
authors.forename: Fábia K
authors.initials: FK
authors.lastname: Andrade
authors.forename: Raquel
authors.initials: R
authors.lastname: Costa
authors.forename: Lucília
authors.initials: L
authors.lastname: Domingues
authors.forename: Raquel
authors.initials: R
authors.lastname: Soares
authors.forename: Miguel
authors.initials: M
authors.lastname: Gama
mesh_terms: D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000737:chemistry; D001419:Bacteria / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D002482:Cellulose / Q000737:chemistry* / Q000235:genetics; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D013499:Surface Properties
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; C047981:arginyl-glycyl-aspartic acid; D002482:Cellulose