Exogenous dendritic cell homing to draining lymph nodes can be boosted by mast cell degranulation.
Dendritic cells (DCs), as potent antigen presenting cells, are increasingly used for immunotherapeutic approaches, predominantly in oncology. Low efficiency of injected Ag-pulsed DC homing to draining lymph nodes (DLNs) is one of the factors that affect the efficacy of therapy. As Langerhans cell emigration was enhanced after skin mast cell degranulation, we investigated the effect of local mast cell activation on exogenous bone marrow-derived DCs (BM-DCs) homing to DLNs. Product of activated MC/9 mast cells enhanced chemotaxis of BM-DCs to CCL21 in vitro. Intradermal injection of compound 48/80 (c48/80) induced local skin mast cell obvious degranulation and boosted exogenous BM-DC homing to DLNs. Both Ag-specific lymphocyte proliferation and TH1/TH2 cytokine production increased after HBsAg-pulsed BM-DC was injected into c48/80 pretreated mice. These results suggest that transferred DC homing to DLNs promoted by local mast cell degranulation may have potential application to improve DC-based immunotherapy.
Ren
Shu-Rong
SR
Department of Immunology, Medical College of Qingdao University, Qingdao 266071, China.
Xu
Li-Bin
LB
Wu
Zhi-Yuan
ZY
Du
Jun
J
Gao
Mei-Hua
MH
Qu
Chun-Feng
CF
D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016201:Receptors, Lymphocyte Homing / Q000276:immunology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D016201:Receptors, Lymphocyte Homing; D003189:p-Methoxy-N-methylphenethylamine