title: KITENIN represents a more aggressive phenotype in a murine model of oral cavity squamous carcinoma.
abstract: OBJECTIVE
  To investigate the tumor invasiveness and early lung metastasis associated with KITENIN in a murine model of oral cavity squamous carcinoma.
  
  
  METHODS
  Cross-sectional study with planned data collection.
  
  
  METHODS
  Department of Otolaryngology-Head and Neck Surgery, Medical Research Center for Gene Regulation, and Pathology, Chonnam National University Medical School and Hwasun Hospital.
  
  
  METHODS
  The cDNA of KITENIN and vector only were transfected into the SCC VII (murine squamous cell line) cells. The suspension of 5 x 10(5)/mL viable KITENIN- or vector-transfected SCC VII cells was injected into the floor of the mouth of C3H/HeJ syngeneic mice, deep into the mylohyoid muscle via the intraoral approach. From the first to the sixth week after injection, tumor, lung, liver, and brain tissues were obtained.
  
  
  RESULTS
  For all groups, the tumor invaded the superficial musculature of the floor of the mouth, the deep musculature of the floor of the mouth, the salivary glands, perineural tissue, bone, and skin, sequentially. Lung metastases developed multiple focal nodular patterns at first and then became more extensive. Earlier and more aggressive tumor invasion into the deep floor of the mouth musculature, salivary glands, perineural tissue, bone, and skin was observed in the KITENIN-transfected group compared to the vector-only group. An earlier and more extensive lung metastasis was observed in the KITENIN group.
  
  
  CONCLUSIONS
  An antisense KITENIN strategy might inhibit distant metastasis and the progression of head and neck squamous cell carcinoma.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School and Hwasun Hospital, Hwasungun, Jeonnam, Korea. joonkyoo@chonnam.ac.kr
authors.forename: Joon Kyoo
authors.initials: JK
authors.lastname: Lee
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Lim
authors.forename: Hee Dae
authors.initials: HD
authors.lastname: Kim
authors.forename: Tae Mi
authors.initials: TM
authors.lastname: Yoon
authors.forename: Kag
authors.initials: K
authors.lastname: Kim
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Nam
authors.forename: Hyung Seok
authors.initials: HS
authors.lastname: Kim
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Shin
authors.forename: Kyung Keun
authors.initials: KK
authors.lastname: Kim
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006258:Head and Neck Neoplasms / Q000473:pathology; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000556:secondary*; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D009062:Mouth Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness; D014162:Transfection
chemical_list: D008565:Membrane Proteins