title: Response to experimentally induced infection with bovine respiratory syncytial virus following intranasal vaccination of seropositive and seronegative calves.
abstract: OBJECTIVE
  To determine whether a combination modified-live bovine respiratory syncytial virus (BRSV) vaccine can stimulate protective immunity in young BRSV-seropositive calves following intranasal (IN) administration.
  
  
  METHODS
  Controlled challenge study.
  
  
  METHODS
  66 Holstein bull calves, 3 to 8 days old.
  
  
  METHODS
  In experiment 1, BRSV-seropositive and -seronegative calves were vaccinated IN with a commercially available combination modified-live virus vaccine formulated for SC administration; calves underwent BRSV challenge 4.5 months later. In experiment 2, BRSV-seronegative calves were vaccinated IN or SC (to examine the effect of route of administration) with the same combination vaccine that instead had a 1/100 dose of BRSV (to examine the effect of dose); calves underwent BRSV challenge 21 days later.
  
  
  RESULTS
  In experiment 1, BRSV challenge resulted in severe respiratory tract disease with low arterial partial pressures of oxygen and lung lesions in most calves from all groups. Maximum change in rectal temperature was significantly greater in seropositive IN vaccinated calves, compared with seronegative IN vaccinated and seropositive control calves. Number of days of BRSV shedding was significantly lower in seronegative IN vaccinated calves than in seropositive IN vaccinated and seropositive control calves. In experiment 2, maximum change in rectal temperature was significantly greater in seronegative control calves, compared with seronegative IN and SC vaccinated calves. Shedding of BRSV was significantly reduced in seronegative IN and SC vaccinated calves, compared with control calves; also, lung lesions were reduced in seronegative IN and SC vaccinated calves.
  
  
  CONCLUSIONS
  Maternal antibodies may inhibit priming of protective responses by IN delivered BRSV vaccines.
authors.affiliation: Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada. john.ellis@usask.ca
authors.forename: John A
authors.initials: JA
authors.lastname: Ellis
authors.forename: Sheryl P
authors.initials: SP
authors.lastname: Gow
authors.forename: Noriko
authors.initials: N
authors.lastname: Goji
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control* / Q000821:virology; D008168:Lung / Q000473:pathology; D008297:Male; D009666:Nose / Q000821:virology; D011024:Pneumonia, Viral / Q000473:pathology / Q000662:veterinary; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control / Q000662:veterinary*; D018112:Respiratory Syncytial Virus, Bovine; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines