title: FNA based diagnosis of head and neck nodal lymphoma.
abstract: Fine-needle aspiration (FNA) biopsy has become a well established technique in the diagnosis, staging, and follow-up of patients with head and neck lesions. As in lymphoma diagnostics, FNA serves as a screening method in evaluating potentially affected lymph node for open or core biopsy. According to the World Health Organization classification of lymphoid neoplasms, today it is important to recognize cell morphology and reveal its phenotype, then combine it with different genotypic information and clinical data to provide appropriate therapy. The aim of this study was to assess the efficacy of FNA and immunocytochemistry based lymphoma diagnostic in head and neck region. We conducted a retrospective study during a period of three years where cases with either FNA diagnosis or clinical suspicion of newly recognized or relapsing lymphoma were reviewed. In the study were included patients that were referred to our laboratory from hematology department, in whom head and neck lymphadenopathia was found and lymph node FNA preceded other procedures. Two hundred eighty-five aspirations from 248 patients fulfilled study criteria. Adequate specimens were diagnosed as lymphoma in 100 cases (36%), in 65 male and 35 female patients, 76 in patients with newly discovered disease and 24 in patients with prior lymphoma diagnosis. Overall sensitivity of FNA specimens in the diagnosis of head and neck lymphomas was 90%, specificity 88%, predictive value of a positive result 97%, and predictive value of negative result 61%. Based on our results FNA corroborated with immunophenotyping by immunocytochemistry can be method of choice in primary lymphoma diagnosis as a method complementary to histopathology in lymphoma diagnostics.
authors.affiliation: Department of Pathology and Cytology, University Hospital Center Zagreb, Zagreb, Croatia. gjadrov@yahoo.co.uk
authors.forename: Koraljka Gjadrov
authors.initials: KG
authors.lastname: Kuvezdić
authors.forename: Igor
authors.initials: I
authors.lastname: Aurer
authors.forename: Suncica
authors.initials: S
authors.lastname: Ries
authors.forename: Mirna
authors.initials: M
authors.lastname: Sucić
authors.forename: Mirjana Marković
authors.initials: MM
authors.lastname: Glamocak
authors.forename: Ivana
authors.initials: I
authors.lastname: Ilić
authors.forename: Sandra
authors.initials: S
authors.lastname: Basić-Kinda
authors.forename: Ivo
authors.initials: I
authors.lastname: Radman
authors.forename: Boris
authors.initials: B
authors.lastname: Labar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044963:Biopsy, Fine-Needle / Q000592:standards*; D002648:Child; D002675:Child, Preschool; D003877:Dermatitis, Contact; D005260:Female; D005500:Follow-Up Studies; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008207:Lymphatic Metastasis / Q000473:pathology; D008223:Lymphoma / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000473:pathology*; D009367:Neoplasm Staging / Q000379:methods / Q000592:standards; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012189:Retrospective Studies; D014944:World Health Organization; D055815:Young Adult