Effects of infrasound on cell proliferation in the dentate gyrus of adult rats.
Adult rats were used to identify the effects of infrasound on neurogenesis in the hippocampal dentate gyrus. After 7 consecutive days' exposure to infrasound of 16 Hz at 130 dB, immunostaining of 5-bromo-2'-deoxyuridine (BrdU) and doublecortin (DCX) was preformed. Compared with those in normal groups, the numbers of BrdU+ and DCX+/BrdU+ cells in the subgranular zone in infrasound groups were significantly decreased at 3, 6, 10 and 14 days and returned to normal at 18 days. The percentage of BrdU+ cells that were co-labeled with DCX showed no significant differences between the infrasound and normal groups. These data suggest that infrasound inhibits the cell proliferation in adult rat dentate gyrus but has no effects on early migration and differentiation of these newborn cells.
Liu
Juanfang
J
Departments of aNeurology bOrthopaedics, Xijing Hospital cInstitute of Neuroscience, Fourth Military Medical University, Xi'an, China.
Lin
Tian
T
Yan
Xiaodong
X
Jiang
Wen
W
Shi
Ming
M
Ye
Ruidong
R
Rao
Zhiren
Z
Zhao
Gang
G
D000161:Acoustic Stimulation / Q000009:adverse effects*; D000162:Acoustics; D000818:Animals; D015415:Biomarkers; D001973:Bromodeoxyuridine; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000166:cytology / Q000502:physiology*; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008297:Male; D008869:Microtubule-Associated Proteins; D055495:Neurogenesis / Q000502:physiology*; D009479:Neuropeptides; D009622:Noise / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013016:Sound / Q000009:adverse effects*; D013997:Time Factors
D015415:Biomarkers; C000714848:Dcx protein, rat; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D001973:Bromodeoxyuridine