Pulmonary kinetic expression of the endothelin system in a swine model of endotoxic shock.
Endothelin (ET)-1 is a potent vasoconstrictor peptide involved in the derangement of respiratory mechanics during endotoxic shock. We measured the kinetics of pulmonary mRNA expression of the key components of the ET system [i.e., ET-1, ET-converting enzyme (ECE), and ETA and ETB receptors] by quantitative real-time reverse transcriptase-polymerase chain reaction in a swine model of endotoxic shock (0, 1, 2, 3, and 4 h of continuous LPS infusion at 40 microg/kg/hour; sham group, 4 hour saline infusion). A significant increase in mRNA expression levels was observed for ET-1 in LPS-treated piglets; the increase began as early as 1 hour. In contrast, no significant variations were observed for the ECE, ETA, or ETB genes. Small gene expression differences observed with respect to our previous results suggest a possible effect of the anesthesia or surgical protocol on ET system regulation.
Zannoni
A
A
Veterinary Medicine Faculty, Department of Veterinary Morphophisiology and Animal Production (DIMORFIPA), University of Bologna, Bologna, Italy. augusta.zannoni@unibo.it
Bernardini
C
C
Gentilini
F
F
Giunti
M
M
Bacci
M L
ML
Forni
M
M
D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000072396:Endothelin-Converting Enzymes; D016232:Endothelins / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D008168:Lung / Q000378:metabolism*; D008297:Male; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism; D044022:Receptor, Endothelin A / Q000235:genetics / Q000378:metabolism; D044023:Receptor, Endothelin B / Q000235:genetics / Q000378:metabolism; D012772:Shock, Septic / Q000378:metabolism / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000139:chemically induced* / Q000378:metabolism
D016232:Endothelins; D008070:Lipopolysaccharides; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; D000072396:Endothelin-Converting Enzymes