Well-defined poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) vectors with low toxicity and high gene transfection efficiency.
Successful gene delivery vectors for clinical translation should have high transfection efficiency and minimal toxicity. In this work, well-defined poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) (PGEA) vectors with flanking cationic secondary amine and nonionic hydroxyl units were prepared via the ring-opening reaction of the pendant epoxide groups of poly(glycidyl methacrylate) with the amine moieties of ethanolamine. It was found that PGEA carriers possess very low toxicity (<10% of the toxicity of branched polyethylenimine (PEI, 25 kDa), while exhibiting surprisingly excellent transfection efficiency (higher than or comparable to that of PEI (25 kDa)) in different cell lines. A series of transfection and cytotoxicity assays revealed that PGEAs are highly promising as a new class of safe and efficient gene delivery vectors for future clinical gene therapies.
Xu
F J
FJ
State Key Laboratory of Chemical Resource Engineering, Ministry of Education, College of Materials Science & Engineering, Beijing University of Chemical Technology, Beijing China 100029. xufj@mail.buct.edu.cn
Chai
M Y
MY
Li
W B
WB
Ping
Y
Y
Tang
G P
GP
Yang
W T
WT
Ma
J
J
Liu
F S
FS
D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003001:Cloning, Molecular; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D018014:Gene Transfer Techniques; D006801:Humans; D008156:Luciferases / Q000235:genetics; D015394:Molecular Structure; D010957:Plasmids; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D048790:Renilla / Q000235:genetics; D014162:Transfection
D004337:Drug Carriers; D011109:Polymethacrylic Acids; C553271:poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate); D004247:DNA; D008156:Luciferases