A closer look at the structure of sterically stabilized liposomes: a small-angle X-ray scattering study.
The evaluation of the radial electron density profile of a drug containing a sterically stabilized liposomal system is described. Using synchrotron small-angle X-ray scattering, we were able to characterize the hydrophilic shell of the polyethylene glycol chains. Using a Gaussian model for describing the electron density profile along the normal of the bilayer, we got an asymmetric distribution of PEGylated lipids in accordance with theoretical considerations. Moreover, we used anomalous X-ray scattering to study the localization of a hydrophobic drug (a kinase inhibitor), which revealed that these molecules are mainly located in the hydrocarbon chain region of the phospholipid bilayer.
Varga
Zoltán
Z
Institute of Nanochemistry and Catalysis, Chemical Research Center, Hungarian Academy of Sciences, Pusztaszeri út 59-67, H-1025 Budapest, Hungary.
Berényi
Szilvia
S
Szokol
Bálint
B
Orfi
László
L
Kéri
György
G
Peták
István
I
Hoell
Armin
A
Bóta
Attila
A
D008081:Liposomes / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
D008081:Liposomes; D010713:Phosphatidylcholines; D011092:Polyethylene Glycols; C028694:1-palmitoyl-2-oleoylphosphatidylcholine