Arsenite oxidase from Ralstonia sp. 22: characterization of the enzyme and its interaction with soluble cytochromes.
We characterized the aro arsenite oxidation system in the novel strain Ralstonia sp. 22, a beta-proteobacterium isolated from soil samples of the Salsigne mine in southern France. The inducible aro system consists of a heterodimeric membrane-associated enzyme reacting with a dedicated soluble cytochrome c(554). Our biochemical results suggest that the weak association of the enzyme to the membrane probably arises from a still unknown interaction partner. Analysis of the phylogeny of the aro gene cluster revealed that it results from a lateral gene transfer from a species closely related to Achromobacter sp. SY8. This constitutes the first clear cut case of such a transfer in the Aro phylogeny. The biochemical study of the enzyme demonstrates that it can accommodate in vitro various cytochromes, two of which, c(552) and c(554,) are from the parent species. Cytochrome c(552) belongs to the sox and not the aro system. Kinetic studies furthermore established that sulfite and sulfide, substrates of the sox system, are both inhibitors of Aro activity. These results reinforce the idea that sulfur and arsenic metabolism are linked.
Lieutaud
Aurélie
A
Laboratoire de Bioénergétique et Ingénierie des Protéines UPR 9036, IFR88, CNRS, F-13402 Marseille Cedex 20, France.
van Lis
Robert
R
Duval
Simon
S
Capowiez
Line
L
Muller
Daniel
D
Lebrun
Régine
R
Lignon
Sabrina
S
Fardeau
Marie-Laure
ML
Lett
Marie-Claire
MC
Nitschke
Wolfgang
W
Schoepp-Cothenet
Barbara
B
D000595:Amino Acid Sequence; D001149:Arsenates / Q000378:metabolism; D001151:Arsenic / Q000378:metabolism; D003580:Cytochromes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005784:Gene Amplification; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010088:Oxidoreductases / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics; D043367:Ralstonia / Q000201:enzymology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013104:Spheroplasts / Q000201:enzymology
D001149:Arsenates; D003580:Cytochromes; D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021122:Protein Subunits; D010088:Oxidoreductases; C078179:arsenite oxidase; D001151:Arsenic