title: Probiotic preparation reduces the faecal water genotoxicity in chickens fed with aflatoxin B1 contaminated fodder.
abstract: The aim of the study was to evaluate the influence of a probiotic preparation on the genotoxicity of faecal water of broiler chickens fed with a fodder contaminated with aflatoxin B(1) (AFB(1)) at 1 or 5mg per kg. Human blood lymphocytes were exposed to chicken's faecal water samples and DNA damage was measured using the comet assay. Genotoxicity of faecal water did not depend on the AFB(1) concentration in the fodder. The mean DNA damage, measured as the percentage of DNA in the tail of the comets, for chickens fed with fodder with AFB(1) at 1 mg/kg was 16.80±0.66, at 5 mg/kg - 16.73±1.51 and in the controls - 12.79±0.66. The supplementation of fodder with the probiotic preparation decreased the extent of DNA damage to 10.02±0.39 for 1 mg/kg AFB(1) and to 11.89±0.72 for 5 mg/kg.
authors.affiliation: Institute of Fermentation Technology and Microbiology, Department of Biotechnology and Food Sciences, Technical University of Lodz, ul. Wolczanska 171/173, 90-924 Lodz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Slizewska
authors.forename: Adriana
authors.initials: A
authors.lastname: Nowak
authors.forename: Zdzislawa
authors.initials: Z
authors.lastname: Libudzisz
authors.forename: Janusz
authors.initials: J
authors.lastname: Blasiak
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000821:Animal Feed / Q000009:adverse effects* / Q000382:microbiology; D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D002645:Chickens / Q000502:physiology*; D020552:Comet Assay / Q000662:veterinary; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics; D019587:Dietary Supplements; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009152:Mutagenicity Tests / Q000662:veterinary; D011201:Poultry Diseases / Q000517:prevention & control*; D019936:Probiotics / Q000627:therapeutic use*
chemical_list: D016604:Aflatoxin B1