[Distraction osteogenesis for correction of cleft palate in rhesus-histological and fluorescent labeling study].
OBJECTIVE
To investigate the mechanism of new bone formation in the distraction osteogenesis (DO) for correction of cleft palate (CP) in rhesus.


METHODS
CP was created by operation in 23 rhesus. The CP was corrected with DO in 21 animals as experimental group. The distraction rate was 0.8 mm per day, two times a day. The bone fragments were fixed after cleft closure, every 3 animals were sacrificed to get specimen after 1, 2, 4, 6, 8, 12, 24 weeks of fixation. 6 days before sacrifice, tetracycline was administrated for labeling (30 mg/kg).


RESULTS
The hard and soft tissue def of fixation. At the same time, the bone volume and calcification between the distraction gap increased. The cleft in the control group could not b ect was successfully closed with DO by intramembrane osteogenesis. The new formed bone was remodeling and became maturation during the period e corrected spontaneously.


CONCLUSIONS
The DO can successfully correct both the soft and hard tissue defect in CP by intramembrane osteogenesis. The fixation is important for remodeling and maturation of the new formed bone.
Liu
Yi
Y
Department of Oral and Maxillofacial Surgery, the Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China.
Chen
Gang
G
Liu
Yan-shan
YS
Shen
Dai
D
Zhu
Tong
T
Wang
Zhi-qi
ZQ
D000818:Animals; D015415:Biomarkers; D002972:Cleft Palate / Q000473:pathology* / Q000601:surgery*; D008251:Macaca; D019857:Osteogenesis, Distraction; D021362:Palate, Hard / Q000473:pathology; D010160:Palate, Soft / Q000473:pathology
D015415:Biomarkers