Proteomic and metabolomic profiles in atherothrombotic vascular disease.
Atherothrombosis remains a major cause of morbidity and mortality in the western world. The underlying processes associated with clinical expression of atherothrombosis include oxidative stress and proteolysis in relation to neovascularisation and intraplaque hemorrhages, leading to immuno-inflammatory response, cell death, and extracellular matrix breakdown. The complex biological multifactorial nature of atherothrombosis requires the development of novel technologies that allow the analysis of cellular and molecular processes responsible for the transition to disease phenotypes and the discovery of new diagnostic and prognostic biomarkers. In the present article, we have reviewed recent advances in the application of proteomic and metabolomic techniques to the study of atherothrombosis. We have focused on recent studies analyzing cells involved in hemo-thrombus formation (platelets, red blood cells, and polymorphonuclear cells), as well as tissues, tissue-conditioned media, and plasma of atherothrombotic patients. In the future, the application of these high-throughput technologies, along with imaging techniques, in systems biology approaches will help to individualize medicine.
Martinez-Pinna
Roxana
R
Vascular Research Laboratory, Instituto de Investigacion Sanitaria, Fundación Jimenez Diaz, Autonoma University, Av. Reyes Católicos 2, 28040, Madrid, Spain.
Barbas
Coral
C
Blanco-Colio
Luis Miguel
LM
Tunon
Jose
J
Ramos-Mozo
Priscila
P
Lopez
Juan Antonio
JA
Meilhac
Olivier
O
Michel
Jean-Baptiste
JB
Egido
Jesus
J
Martin-Ventura
José Luis
JL
D050197:Atherosclerosis / Q000150:complications* / Q000378:metabolism; D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D004730:Endothelium, Vascular / Q000737:chemistry; D006801:Humans; D055432:Metabolomics; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D013927:Thrombosis / Q000209:etiology* / Q000378:metabolism
D015415:Biomarkers; D001798:Blood Proteins; D011506:Proteins