Biosurfactants as agents to reduce adhesion of pathogenic bacteria to polystyrene surfaces: effect of temperature and hydrophobicity.
Polystyrene surfaces were conditioned with surfactin and rhamnolipid biosurfactants and then assessed regarding the attachment of Staphylococcus aureus, Listeria monocytogenes, and Micrococcus luteus. The effect of different temperatures (35, 25, and 4°C) on the anti-adhesive activity was also studied. Microbial adhesion to solvents and contact angle measurements were performed to characterize bacteria and material surfaces. The results showed that surfactin was able to inhibit bacterial adhesion in all the conditions analyzed, giving a 63-66% adhesion reduction in the bacterial strains at 4°C. Rhamnolipid promoted a slight decrease in the attachment of S. aureus. The anti-adhesive activity of surfactin increased with the decrease in temperature, showing that this is an important parameter to be considered in surface conditioning tests. Surfactin showed good potential as an anti-adhesive compound that can be explored to protect surfaces from microbial contamination.
Zeraik
Ana Eliza
AE
Chemistry Institute of São Carlos, University of São Paulo, Avenida Trabalhador São-Carlense, 400, PO Box 780, São Carlos, SP, CEP 13560-970, Brazil.
Nitschke
Marcia
M
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000187:drug effects*; D004783:Environmental Microbiology; D006017:Glycolipids; D057927:Hydrophobic and Hydrophilic Interactions; D055666:Lipopeptides / Q000378:metabolism; D008089:Listeria monocytogenes / Q000187:drug effects / Q000502:physiology; D016982:Micrococcus luteus / Q000187:drug effects / Q000502:physiology; D010456:Peptides, Cyclic / Q000378:metabolism; D011137:Polystyrenes; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology; D013501:Surface-Active Agents / Q000378:metabolism*; D013696:Temperature
D000900:Anti-Bacterial Agents; D006017:Glycolipids; D055666:Lipopeptides; D010456:Peptides, Cyclic; D011137:Polystyrenes; D013501:Surface-Active Agents; C418382:rhamnolipid; C009365:surfactin peptide