Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells.
MicroRNAs (miRNAs) are a family of endogenous small non-coding RNAs which regulate mRNAs at the post-transcriptional level. MiRNAs have been identified in both normal physiological and pathological conditions. To date, a limited number of miRNAs have been shown to be involved in the regulation of insulin secretion. We have identified a panel of 10 miRNAs down-regulated in glucose non-responsive MIN6 cells compared to glucose responsive cells using TaqMan Low Density miRNA Arrays. Of these 10 miRNA targets, subsequent functional investigations involving knockdown of mir-200a, mir-130a and mir-410 levels suggested that they may decrease the capability of MIN6 cells to secrete insulin in response to stimulatory levels of glucose. Conversely, experiment with over-expression of mir-410 suggest that it may enhance levels of glucose stimulated insulin secretion. In this study, we have also identified 21 miRNAs not previously known to have a potential murine homologue.
Hennessy
Erica
E
National Institute of Cellular Biotechnology, Dublin City University, Dublin 9, Ireland. Erica.hennessy4@mail.dcu.ie
Clynes
Martin
M
Jeppesen
Per Bendix
PB
O'Driscoll
Lorraine
L
D000818:Animals; D045744:Cell Line, Tumor; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000235:genetics / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
D007328:Insulin; D035683:MicroRNAs; D005947:Glucose