Size and dynamics of the Vibrio cholerae porins OmpU and OmpT probed by polymer exclusion.
The trimeric OmpU and OmpT porins form large, triple-barrel hydrophilic channels in the outer membrane of the pathogen Vibrio cholerae. They have distinct pore properties, such as conductance, block by deoxycholic acid, and sensitivity to acidic pH. Their three-dimensional structures are unknown, but they share significant sequence homologies. To gain insight into the molecular basis for the distinct functional properties of these two similar porins, we carried out polymer exclusion experiments using planar lipid bilayer and patch-clamp electrophysiology. By studying the partitioning of polyethylene glycols (PEGs) of different molecular weights into each porin, we determined an effective radius of 0.55 nm and 0.43 nm for OmpU and OmpT respectively, and found an increased OmpU effective radius at acidic pH. PEGs or high buffer ionic strength promotes the appearance of single step closures in OmpU similar to the acidic-pH induced closures we documented previously. In addition, these closing events can be triggered by nonpenetrating PEGs applied asymmetrically. We believe our results support a model whereby acidic pH, high ionic strength, or exposure to PEGs stabilizes a less conductive state that corresponds to the appearance of an additional resistive element on one side of the OmpU protein and common to the three monomers.
Duret
Guillaume
G
Department of Biology and Biochemistry, University of Houston, Houston, Texas, USA.
Delcour
Anne H
AH
D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D004553:Electric Conductivity; D006863:Hydrogen-Ion Concentration; D015640:Ion Channel Gating; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism*; D018272:Porins / Q000737:chemistry* / Q000378:metabolism*; D016062:Porosity; D014734:Vibrio cholerae
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; C096349:OmpU protein, Vibrio cholerae; D018272:Porins; C405564:ompT protein, bacteria; D011092:Polyethylene Glycols