Sequence analysis of the gene encoding H antigen in Escherichia coli isolated from food in Morocco.
In order to develop other molecular method useful for typing of motile and non motile Escherichia coli strains, a total of 207 strains of E. coli (133 reference strains, 74 food strains) were characterized by analysis of sequences of their amplified flagellin-encoding (fliC) gene products. The collection of reference strains was used for database building of fliC gene sequences. Application of this identification system to 74 E. coli food isolates revealed a reproducible and clear cut classification with very good correlation to results obtained by HhaI restriction of the amplified flagellin gene. The proposed determination of fliC sequences variations should be helpful for epidemiological studies.
Badri
Samira
S
Laboratoire de Microbiologie et d'Hygiène des Aliments et de l'Environnement, Institut Pasteur du Maroc, Casablanca, 20100, Morocco. samira_bdm@yahoo.fr
Fassouane
Aziz
A
Filliol
Ingrid
I
Hassar
Mohammed
M
Cohen
Nozha
N
D000942:Antigens, Bacterial / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005408:Flagellin; D005516:Food Microbiology; D005838:Genotype; D008969:Molecular Sequence Data; D009018:Morocco; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D017421:Sequence Analysis
D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; C022863:H antigen; D005408:Flagellin; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases