[Inhibitive effect of tetrandrine on the proliferation of diabetes mellitus rats vascular smooth muscle cells].
OBJECTIVE
To investigate the effects of tetrandrine on nuclear factor-kappaB (NF-kappaB) activity and proliferation of Diabetes Mellitus (DM) rats vascular smooth muscle cells (VSMCs).


METHODS
DM models were established,the culture of DM and normal VSMCs were done by modified tissue-piece incoculztion; Inhibitory effect of Tetrandrine was determined by cell counting kit (CCK-8). Immunofluorescence laser confocal microscopy was used to estimate the effect of NF-kappaB in DM VSMCs with Tet.


RESULTS
Tetrandrine inhibited the proliferation of DM and normal VSMCs,the IC50 value was (11.35 +/- 1.24) micromol/L and (28.01 +/- 4.35) micromol/L, respectively. Furthermore, Tetrandrine strongly inhibited the activation of NF-kappaB in DM VSMCs and reduced the nuclear translocation of NF-kappaB.


CONCLUSIONS
Tetrandrine inhibits the proliferation of DM VSMCs more effectively than that of normal, the mechanism may be related to reducing NF-kappaB nuclear translocation.
Liu
Ming-hua
MH
Department of pharmacology, Luzhou Medical College, Luzhou 646000, China. liumhvip@163.com
Xie
Wei-rong
WR
Zhang
Zhuo
Z
Cao
Yang
Y
Ren
Mei-ping
MP
Xiao
Shun-han
SH
D000818:Animals; D044182:Benzylisoquinolines / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D031609:Stephania tetrandra / Q000737:chemistry*; D013311:Streptozocin
D044182:Benzylisoquinolines; D002121:Calcium Channel Blockers; D016328:NF-kappa B; C009438:tetrandrine; D013311:Streptozocin