The effect of TCDD dioxin on the rat liver in biochemical and histological assessment.
Eighteen male Wistar rats were divided into 3 groups of 6 animals each. Two groups received different intraperitoneal doses of TCDD (0.75 and 8 microg) in DMSO solution and the third group (control) received only DMSO on days 0, 7 and 14. On day 21 the animals were sacrificed, and then blood tests, pathological examination and CYP1A1 activity measurement were performed. In rats that received a high dose of dioxin (8 microg) hepatic lobules revealed parenchymal degeneration and vacuolization of hepatocytes was observed, and also an increased CYP reaction was found in central parts of lobules, around the central vein. The reaction in control and low dose groups was weak. The resorufin level was significantly (P < 0.05) higher in the group receiving a low dose of dioxin as compared to the control group. The study confirmed that TCDD damages the rat liver in a dose-dependent manner. Administration of high TCDD doses causing major liver damage also damaged CYP1A1 (based on higher resorufin levels in epiluminescence). TCDD activates CYP1A1, which was confirmed by increased immunohistochemical reactivity of central areas ofhepatic lobules.
Czepiel
Jacek
J
Jagiellonian University Medical College, Sniadeckich 5, 31-501 Kraków, Poland. jacz@op.pl
Biesiada
Grazyna
G
Gajda
Mariusz
M
Szczepański
Wojciech
W
Szypuła
Kinga
K
Dabrowski
Zbigniew
Z
Mach
Tomasz
T
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism* / Q000473:pathology*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D004121:Dimethyl Sulfoxide; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry; D008297:Male; D010078:Oxazines / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar
D004785:Environmental Pollutants; D010078:Oxazines; D000072317:Polychlorinated Dibenzodioxins; C445379:1,2,7,8-tetrachlorodibenzo-p-dioxin; C014180:resorufin; D019363:Cytochrome P-450 CYP1A1; D004121:Dimethyl Sulfoxide