Rapid differentiation of seasonal and pandemic H1N1 influenza through proteotyping of viral neuraminidase with mass spectrometry.
Signature peptides of the neuraminidase antigen across all common circulating human subtypes of type A and B influenza are identified through the bioinformatic alignment of translated gene sequences. The detection of these peptides within the high-resolution mass spectra of whole antigen, virus, and vaccine digests enables the strains to be rapidly and directly typed and subtyped. Importantly, unique signature peptides for pandemic (H1N1) 2009 influenza are identified and detected that enable pandemic strains to be rapidly and directly differentiated from seasonal type A (H1N1) influenza strains. The detection of these peptides can enable the origins of the neuraminidase gene to be monitored in the case of reassorted strains.
Schwahn
Alexander B
AB
School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia.
Wong
Jason W H
JW
Downard
Kevin M
KM
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000032:analysis / Q000737:chemistry / Q000145:classification; D004196:Disease Outbreaks; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification* / Q000201:enzymology*; D053122:Influenza A Virus, H3N2 Subtype / Q000145:classification / Q000201:enzymology; D007251:Influenza, Human / Q000453:epidemiology*; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000032:analysis / Q000737:chemistry / Q000145:classification*; D012621:Seasons; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D000956:Antigens, Viral; D009439:Neuraminidase