Analytical procedure for the determination of eprinomectin in soil and cattle faeces.
A new analytical HPLC-fluorescence method was developed for the quantitative determination of eprinomectin (EPM) in soil and cattle faeces. EPM was extracted with acetone and acetonitrile from soil and cattle faeces, respectively. Solid phase extraction and derivatization reaction with N-methylimidazole in the presence of trifluoroacetic anhydride and acetic acid were applied. The limit of quantitation was 1 ng g(-1) air dried soil and 2.5 ng g(-1) moist cattle faeces. Overall recovery (RSD) was 89% (8) in soil and 85% (10) in cattle faeces and its good reproducibility (RSD<15%) allows the application of the method in advanced ecotoxicological studies, required for the environmental fate assessment of EPM.
Litskas
V D
VD
Laboratory of Ecology and Environmental Protection, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Greece. vlitskas@vet.auth.gr
Batzias
G C
GC
Karamanlis
X N
XN
Kamarianos
A P
AP
D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005243:Feces / Q000737:chemistry*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis; D015203:Reproducibility of Results; D012987:Soil / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods
D012987:Soil; D007559:Ivermectin; C101434:eprinomectin