Potentializing the effects of GM1 by hyperbaric oxygen therapy in acute experimental spinal cord lesion in rats.
METHODS
Experimental, controlled, animal study.


OBJECTIVE
To evaluate the effect of GM1 ganglioside, hyperbaric oxygen and both in combination, in the treatment of experimental spinal cord lesions in rats.


METHODS
Brazil.


METHODS
Thirty-two Wistar rats with spinal cord lesions were divided into four groups: one group received GM1 ganglioside, one was submitted to hyperbaric oxygen therapy (HBOT), the third received both treatments and the fourth received no treatment (control).


RESULTS
There were no significant differences between the groups in the histological analysis, for any of the variables (necrosis, hemorrhage, hyperemia, cystic degeneration, P>0.06). Neither were there any significant differences in the comparison of left and right sides in the functional tests (P>0.06 for all). No significant differences were found in the locomotor ratings, in the comparison of groups at 2, 7, 21 and 28 days after the surgical procedure. However, in the evaluation on day 14, group 3, which received the combined therapy, showed a significantly higher Basso Beattie and Bresnahan score than the other groups (P=0.015).


CONCLUSIONS
The therapeutic effect of GM1 in locomotor evaluation of rats submitted to spinal cord lesion is anticipated by HBOT.
Marcon
R M
RM
Department of Orthopaedics and Traumatology, University São Paulo Medicine School, São Paulo, Brazil. marconrm@me.com
Cristante
A F
AF
de Barros Filho
T E P
TE
de Oliveira
R P
RP
dos Santos
G B
GB
D000208:Acute Disease; D000818:Animals; D003131:Combined Modality Therapy / Q000379:methods; D004195:Disease Models, Animal; D005677:G(M1) Ganglioside / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D006931:Hyperbaric Oxygenation / Q000379:methods*; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism
D018696:Neuroprotective Agents; D005677:G(M1) Ganglioside