title: Screening and evaluation of human intestinal lactobacilli for the development of novel gastrointestinal probiotics.
abstract: The aim of this study was to screen intestinal lactobacilli strains for their advantageous properties to select those that could be used for the development of novel gastrointestinal probiotics. Ninety-three isolates were subjected to screening procedures. Fifty-nine percent of the examined lactobacilli showed the ability to auto-aggregate, 97% tolerated a high concentration of bile (2% w/v), 50% survived for 4 h at pH 3.0, and all strains were unaffected by a high concentration of pancreatin (0.5% w/v). One Lactobacillus buchneri strain was resistant to tetracycline. None of the tested strains caused lysis of human erythrocytes. Six potential probiotic strains were selected for safety evaluation in a mouse model. Five of 6 strains caused no translocation, and were considered safe. In conclusion, several strains belonging to different species and fermentation groups were found that have properties required for a potential probiotic strain. This study was the first phase of a multi-phase study aimed to develop a novel, safe and efficient prophylactic and therapeutic treatment system against gastrointestinal infections using genetically modified probiotic lactobacilli.
authors.affiliation: Department of Microbiology, University of Tartu, Tartu, Estonia.
authors.forename: Piret
authors.initials: P
authors.lastname: Kõll
authors.forename: Reet
authors.initials: R
authors.lastname: Mändar
authors.forename: Imbi
authors.initials: I
authors.lastname: Smidt
authors.forename: Pirje
authors.initials: P
authors.lastname: Hütt
authors.forename: Kai
authors.initials: K
authors.lastname: Truusalu
authors.forename: Raik-Hiio
authors.initials: RH
authors.lastname: Mikelsaar
authors.forename: Jelena
authors.initials: J
authors.lastname: Shchepetova
authors.forename: Kasper
authors.initials: K
authors.lastname: Krogh-Andersen
authors.forename: Harold
authors.initials: H
authors.lastname: Marcotte
authors.forename: Lennart
authors.initials: L
authors.lastname: Hammarström
authors.forename: Marika
authors.initials: M
authors.lastname: Mikelsaar
mesh_terms: D000143:Acids / Q000633:toxicity; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity; D018988:Bacterial Translocation; D001646:Bile / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004912:Erythrocytes / Q000382:microbiology; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D010194:Pancreatin / Q000633:toxicity; D019936:Probiotics; D017422:Sequence Analysis, DNA
chemical_list: D000143:Acids; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D010194:Pancreatin