Genomic structure and promoter analysis of the dsz operon for dibenzothiophene biodesulfurization from Gordonia alkanivorans RIPI90A.
The bacterium Gordonia alkanivorans RIPI90A has been previously reported as dibenzothiophene-desulfurizing strain. The present study provides a complete investigation of the dsz operon including dsz promoter analysis from desulfurization competent strain belonging to the genus Gordonia. PCR was used to amplify the dszABC genes and adaptor ligation-based PCR-walking strategy used to isolate the dsz promoter. Unlike the dsz operon of Rhodococcus erythropolis, the operon of RIPI90A was located on chromosome. Despite the remarkably high homology between dsz genes of G. alkanivorans RIPI90A and R. erythropolis IGST8, promoter sequences of the strains were not very similar. The dsz promoter of G. alkanivorans RIPI90A shows only 52.5% homology to that of R. erythropolis IGTS8 and Gordonia nitida. Deletion analysis of the dsz promoter from RIPI90A using luciferase as a reporter gene revealed that the dsz promoter was located in regions from -156 to -50.
Shavandi
Mahmoud
M
Department of Biotechnology, Biotechnology Research Center, Research Institute of Petroleum Industry, West Blvd. Azadi Sport Complex, Tehran, Iran. Shavandim@ripi.ir
Sadeghizadeh
Majid
M
Khajeh
Khosro
K
Mohebali
Ghasemali
G
Zomorodipour
Alireza
A
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D016680:Genome, Bacterial; D033601:Gordonia Bacterium / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D013876:Thiophenes / Q000378:metabolism*
D001426:Bacterial Proteins; D013876:Thiophenes; D010105:Oxygenases; C421210:dibenzothiophene sulfone monooxygenase; C016366:dibenzothiophene