title: Biogenic nanoporous silica-based sensor for enhanced electrochemical detection of cardiovascular biomarkers proteins.
abstract: The goal of our research is to demonstrate the feasibility of employing biogenic nanoporous silica as a key component in developing a biosensor platform for rapid label-free electrochemical detection of cardiovascular biomarkers from pure and commercial human serum samples with high sensitivity and selectivity. The biosensor platform consists of a silicon chip with an array of gold electrodes forming multiple sensor sites and works on the principle of electrochemical impedance spectroscopy. Each sensor site is overlaid with a biogenic nanoporous silica membrane that forms a high density of nanowells on top of each electrode. When specific protein biomarkers: C-reactive protein (CRP) and myeloperoxidase (MPO) from a test sample bind to antibodies conjugated to the surface of the gold surface at the base of each nanowell, a perturbation of electrical double layer occurs resulting in a change in the impedance. The performance of the biogenic silica membrane biosensor was tested in comparison with nanoporous alumina membrane-based biosensor and plain metallic thin film biosensor. Significant enhancement in the sensitivity and selectivity was achieved with the biogenic silica biosensor, in comparison to the other two, for detecting the two protein biomarkers from both pure and commercial human serum samples. The sensitivity of the biogenic silica biosensor is approximately 1 pg/ml and the linear dose response is observed over a large dynamic range from 1 pg/ml to 1 microg/ml. Based on its performance metrics, the biogenic silica biosensor has excellent potential for development as a point of care handheld electronic biosensor device for detection of protein biomarkers from clinical samples.
authors.affiliation: School of Mechanical, Aerospace, Chemical, and Materials Engineering, Arizona State University, Tempe, AZ 85287-6106, USA.
authors.forename: Kai-Chun
authors.initials: KC
authors.lastname: Lin
authors.forename: Vindhya
authors.initials: V
authors.lastname: Kunduru
authors.forename: Manish
authors.initials: M
authors.lastname: Bothara
authors.forename: Kaushal
authors.initials: K
authors.lastname: Rege
authors.forename: Shalini
authors.initials: S
authors.lastname: Prasad
authors.forename: B L
authors.initials: BL
authors.lastname: Ramakrishna
mesh_terms: D015415:Biomarkers / Q000097:blood; D040761:Biomimetic Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D002318:Cardiovascular Diseases / Q000097:blood* / Q000175:diagnosis*; D003217:Conductometry / Q000295:instrumentation*; D016207:Cytokines / Q000097:blood*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation; D016062:Porosity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D012822:Silicon Dioxide