N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride nanoparticle as a novel delivery system for parathyroid hormone-related protein 1-34.
Chitosan (CS) and epoxy propyl trimethyl ammonium chloride (EPTAC) were used to prepare the water-soluble N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC). HTCC and sodium tripolyphosphate (TPP) were mixed to form HTCC nanoparticles based on ionic gelation. Parathyroid hormone-related protein 1-34 (PTHrP1-34) was incorporated into the HTCC nanoparticles. The particle size and morphology of nanoparticles were determined by transmission electron microscopy (TEM). HTCC/PTHrP1-34 nanoparticles were 100-180 nm in size and their encapsulation efficiency and loading capacity were related to HTCC concentration, TPP concentration and initial concentration of PTHrP1-34. Relatively optimum encapsulation efficiency (78.4%) and loading capacity (13.7%) of PTHrP1-34 is achieved, and the in vitro release profile of PTHrP1-34 from nanoparticles has an initial burst, which is followed up by a slow release phase. These studies showed that HTCC/PTHrP1-34 nanoparticles are suitable for the treatment of osteoporosis, because of their slow-continuous-release properties, and the relevant in vivo experiments and clinical trials should be further studied.
Zhao
Sheng-hao
SH
Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Wu
Xiao-ting
XT
Guo
Wei-chun
WC
Du
Yu-min
YM
Yu
Ling
L
Tang
Jin
J
D050071:Bone Density Conservation Agents / Q000737:chemistry* / Q000627:therapeutic use; D002626:Chemistry, Pharmaceutical; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004339:Drug Compounding; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D010024:Osteoporosis / Q000188:drug therapy; D044162:Parathyroid Hormone-Related Protein / Q000737:chemistry* / Q000627:therapeutic use; D010316:Particle Size; D010446:Peptide Fragments / Q000737:chemistry* / Q000627:therapeutic use; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods; D014867:Water / Q000737:chemistry
D050071:Bone Density Conservation Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; C487756:N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments; D000644:Quaternary Ammonium Compounds; D014867:Water; C054296:parathyroid hormone-related protein (1-34); D002686:Chitin