title: Targeted gene deletions in C. elegans using transposon excision.
abstract: We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.
authors.affiliation: Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City, Utah, USA.
authors.forename: Christian
authors.initials: C
authors.lastname: Frøkjaer-Jensen
authors.forename: M Wayne
authors.initials: MW
authors.lastname: Davis
authors.forename: Gunther
authors.initials: G
authors.lastname: Hollopeter
authors.forename: Jon
authors.initials: J
authors.lastname: Taylor
authors.forename: Todd W
authors.initials: TW
authors.lastname: Harris
authors.forename: Paola
authors.initials: P
authors.lastname: Nix
authors.forename: Rachel
authors.initials: R
authors.lastname: Lofgren
authors.forename: Michael
authors.initials: M
authors.lastname: Prestgard-Duke
authors.forename: Michael
authors.initials: M
authors.lastname: Bastiani
authors.forename: Donald G
authors.initials: DG
authors.lastname: Moerman
authors.forename: Erik M
authors.initials: EM
authors.lastname: Jorgensen
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D004260:DNA Repair; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000502:physiology; D017353:Gene Deletion; D019895:Transposases / Q000502:physiology
chemical_list: D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C111652:mariner transposases; D019895:Transposases