Structure of the Newcastle disease virus F protein in the post-fusion conformation.
The paramyxovirus F protein is a class I viral membrane fusion protein which undergoes a significant refolding transition during virus entry. Previous studies of the Newcastle disease virus, human parainfluenza virus 3 and parainfluenza virus 5 F proteins revealed differences in the pre- and post-fusion structures. The NDV Queensland (Q) F structure lacked structural elements observed in the other two structures, which are key to the refolding and fusogenic activity of F. Here we present the NDV Australia-Victoria (AV) F protein post-fusion structure and provide EM evidence for its folding to a pre-fusion form. The NDV AV F structure contains heptad repeat elements missing in the previous NDV Q F structure, forming a post-fusion six-helix bundle (6HB) similar to the post-fusion hPIV3 F structure. Electrostatic and temperature factor analysis of the F structures points to regions of these proteins that may be functionally important in their membrane fusion activity.
Swanson
Kurt
K
Howard Hughes Medical Institute, USA.
Wen
Xiaolin
X
Leser
George P
GP
Paterson
Reay G
RG
Lamb
Robert A
RA
Jardetzky
Theodore S
TS
D018360:Crystallography, X-Ray; D008854:Microscopy, Electron; D008958:Models, Molecular; D009522:Newcastle disease virus / Q000737:chemistry* / Q000648:ultrastructure; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D014760:Viral Fusion Proteins / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure
D014760:Viral Fusion Proteins