Influence of the live cell DNA marker DRAQ5 on chromatin-associated processes.
In the last decade, live cell fluorescence microscopy experiments have revolutionized cellular and molecular biology, enabling the localization of proteins within cellular compartments to be analysed and to determine kinetic parameters of enzymatic reactions in living nuclei to be measured. Recently, in vivo DNA labelling by DNA-stains such as DRAQ5, has provided the opportunity to measure kinetic reactions of GFP-fused proteins in targeted areas of the nucleus with different chromatin compaction levels. To verify the suitability of combining DRAQ5-staining with protein dynamic measurements, we have tested the cellular consequences of DRAQ5 DNA intercalation. We show that DRAQ5 intercalation rapidly modifies both the localization and the mobility properties of several DNA-binding proteins such as histones, DNA repair, replication and transcription factors, by stimulating a release of these proteins from their substrate. Most importantly, the effect of DRAQ5 on the mobility of essential cellular enzymes results in a potent inhibition of the corresponding cellular functions. From these observations, we suggest that great caution must be used when interpreting live cell data obtained using DRAQ5.
Mari
Pierre-Olivier
PO
Department of Genetics, Erasmus MC, GE Rotterdam, The Netherlands.
Verbiest
Vincent
V
Sabbioneda
Simone
S
Gourdin
Audrey M
AM
Wijgers
Nils
N
Dinant
Christoffel
C
Lehmann
Alan R
AR
Vermeulen
Wim
W
Giglia-Mari
Giuseppina
G
D000880:Anthraquinones / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007364:Intercalating Agents / Q000737:chemistry* / Q000494:pharmacology; D013194:Staining and Labeling / Q000379:methods; D013312:Stress, Physiological; D014158:Transcription, Genetic / Q000187:drug effects
C520537:1,5-bis((2-(methylamino)ethyl)amino)-4,8-dihydroxyanthracene-9,10-dione; D000880:Anthraquinones; D002843:Chromatin; D004268:DNA-Binding Proteins; D006657:Histones; D007364:Intercalating Agents; D004247:DNA