Simultaneous determination of residues of trichlorfon and dichlorvos in animal tissues by LC-MS/MS.
Trichlorfon is a thermally unstable insecticide that can be easily decomposed to dichlorvos at high temperatures. In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of the residues of trichlorfon and dichlorvos in animal tissues. Trichlorfon and dichlorvos in animal tissues were extracted with dichloromethane, homogenized/shaken, concentrated, and determined by LC-MS/MS. The two insecticides can be effectively separated and individually determined. The limits of detection (LODs) of trichlorfon and dichlorvos were 0.04 and 0.07 microg kg(-1), respectively; the limits of quantification (LOQs) of the two insecticides were both 5 microg kg(-1). The average recoveries for three spiked blank samples at 10, 20 and 40 microg kg(-1) were in the range 85-106%, with relative standard deviations (RSDs) below 10.6%, which falls in a reasonable range for the analysis of the two insecticides in animal-derived foods. The method is fit-for-purpose for the simultaneous determination of residues of trichlorfon and dichlorvos in animal tissues.
Wang
G M
GM
Key Laboratory of Biorheology Science and Technology Under Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
Dai
H
H
Li
Y G
YG
Li
X L
XL
Zhang
J Z
JZ
Zhang
L
L
Fu
Y Y
YY
Li
Z G
ZG
D053000:Analytic Sample Preparation Methods; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004006:Dichlorvos / Q000032:analysis* / Q000737:chemistry; D005506:Food Contamination / Q000517:prevention & control; D005513:Food Inspection / Q000379:methods*; D007306:Insecticides / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis; D018482:Muscle, Skeletal / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D034421:Sus scrofa; D053719:Tandem Mass Spectrometry; D014236:Trichlorfon / Q000032:analysis* / Q000737:chemistry
D007306:Insecticides; D010573:Pesticide Residues; D004006:Dichlorvos; D014236:Trichlorfon