Peptidases of pinworms Syphacia muris and Passalurus ambiguus.
In this first report about pinworms peptidases we primarily characterize peptidases released during in vitro maintenance of two common pinworms of laboratory animals -Syphacia muris and Passalurus ambiguus. The peptidase activity obtained using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed the presence of peptidases from S. muris with a wide range of molecular size (25-110 kDa), which degrades gelatin and mucin at alkaline pH levels. P. ambiguus released serine and aspartyl peptidases degrading gelatin at all tested pH (3, 5, 7, and 9) and at acidic pH Passalurus released aspartyl and cysteine peptidases which are active against mucin.
Vadlejch
Jaroslav
J
Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21 Prague 6 - Suchdol, Czech Republic. vadlejch@af.czu.cz
Lytvynets
Andriy
A
Jankovská
Ivana
I
Langrová
Iva
I
D000818:Animals; D000830:Animals, Laboratory; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005780:Gelatin / Q000378:metabolism; D006790:Host-Parasite Interactions / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008297:Male; D008970:Molecular Weight; D009077:Mucins / Q000378:metabolism; D010123:Oxyuriasis / Q000469:parasitology*; D010124:Oxyuroidea / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D013379:Substrate Specificity; D013450:Sulfones / Q000494:pharmacology
D009077:Mucins; D011480:Protease Inhibitors; D013450:Sulfones; C002010:4-(2-aminoethyl)benzenesulfonylfluoride; D005780:Gelatin; D004492:Edetic Acid; D010447:Peptide Hydrolases