Comprehensive two-dimensional GC of supercritical fluid and pressurized liquid extracts of tobaccos.
The developments of an analytical strategy based on rapid extraction techniques coupled to comprehensive bi-dimensional gas chromatography (GCxGC) for the characterization of the volatile fraction of tobaccos are presented. The high-peak capacity of GCxGC allows considering global extraction techniques that do not focus on restricted chemical families of products. Thus, potential of pressurized fluid extraction and supercritical fluid extraction (SFE) to provide informative extracts is evaluated and compared. As expected, both techniques enable rapid extraction of compounds of interest. However, because of the easy removal of the extracting fluid, SFE provides more concentrated extracts, allowing a GCxGC direct injection without any concentration step. Moreover, the use of pure carbon dioxide with tuneable extraction power favors the extraction of the compounds of interest while reducing the extraction of interfering heavy compounds. GCxGC conditions, especially concerning the set of columns used, are selected thanks to the comparison of separations obtained on model compounds. The developed methodology is applied not only to compare the three main types of tobaccos but also to discriminate between different tobaccos of the same type.
Vial
Jérôme
J
Laboratoire Environnement et Chimie Analytique, ESPCI-CNRS UMR 7195 PECSA, 10 rue Vauquelin, 75005 Paris, France.
Thiébaut
Didier
D
Sassiat
Patrick
P
Beldean-Galea
Mihail Simion
MS
Gomez Ramos
Maria José
MJ
Cognon
Guillaume
G
Mallipattu
Sreedhar
S
Teillet
Beatrice
B
Bouzige
Marianne
M
D002245:Carbon Dioxide / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D002849:Chromatography, Gas / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D011312:Pressure; D014026:Nicotiana / Q000737:chemistry*
D010936:Plant Extracts; D002245:Carbon Dioxide