[miR-126 modulates the expression of epidermal growth factor-like domain 7 in human umbilical vein endothelial cells in vitro].
OBJECTIVE
To investigate the regulatory effect of miR-126 on epidermal growth factor-like domain 7 (EGFL7) in ECV-304 cells.


METHODS
The miR-126-expressing plasmid targeting EGFL7 (plegfp-N1-miR-126) was constructed and transiently transfected into ECV-304 cells via liposome. The changes in the mRNA and protein expressions of EGFL7 in the transfected cells were analyzed by fluorescence quantitative RT-PCR and Western blotting.


RESULTS
Transfection with the recombinant plasmid plegfp-N1/miR-126 resulted in decreased EGFL7 expression with the passage of time, and the expression reached the lowest level at 48 h after the transfection. The expression of EGFL7 protein was reduced by 67% following the transfection in comparison with the control level, while the transfection with the empty vector resulted in a reduction only by 6.5% relative to the control level.


CONCLUSIONS
miR-126 can downregulate EGFL7 expression at the protein level in ECV-304 cells.
Sun
Yan-qin
YQ
Department of Pathology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. celeryking@163.com
Zhang
Fan
F
Bai
Yi-feng
YF
Guo
Lin-lang
LL
D002135:Calcium-Binding Proteins; D002460:Cell Line; D015536:Down-Regulation / Q000235:genetics; D066255:EGF Family of Proteins; D042783:Endothelial Cells / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014471:Umbilical Veins / Q000166:cytology
D002135:Calcium-Binding Proteins; D066255:EGF Family of Proteins; C527877:EGFL7 protein, human; D016228:Endothelial Growth Factors; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger