Role of calcium signalling and phosphorylations in disruption of the epithelial junctions by Pseudomonas aeruginosa quorum sensing molecule.
In Pseudomonas aeruginosa, cell-cell communication based on acyl-homoserine lactone (HSL) quorum sensing molecules is known to coordinate the production of virulence factors and biofilms by the bacterium. Incidentally, these bacterial signals can also modulate mammalian cell behaviour. We demonstrate here that 3O-C(12)-HSL can induce changes in calcium signalling through influx and release of calcium from thapsigargin-sensitive stores and delocalization of inositol 1,4,5-trisphosphate receptors (IP(3)R), but not of ryanodine receptors (RyR). In parallel, P. aeruginosa 3O-C(12)-HSL disrupts junctions in human Caco-2 cells as evidenced by a reduction of the expression and distribution of ZO-3 and JAM-A. Using co-immunoprecipitation we also found an alteration in the binding of ZO-3 to JAM-A in protein complexes. Moreover, 3O-C(12)-HSL-treatment resulted in tyrosine hyperphosphorylation of ZO-3 and JAM-A. On the contrary, serine and threonine residues of ZO-1 and JAM-A became less phosphorylated after exposition of 3O-C(12)-HSL. The 3O-C(12)-HSL-induced intracellular calcium signalling and alteration in the phosphorylation status of junction proteins furthermore correlated with changes in the association between JAM-A-ZO-3. The calcium inhibitors thapsigargin, xestospongin C, and dantrolene partly prevented the 3O-C(12)-HSL-induced decreases in TER and increases in the paracellular flux of 10kDa dextran. These findings clearly suggest that P. aeruginosa 3O-C(12)-HSL can cause the loss of epithelial barrier function via calcium signalling and further alteration in the phosphorylation status of junction proteins; and that bacterial quorum sensing signals represent inter-kingdom signalling.
Vikström
Elena
E
Division of Medical Microbiology, Department of Clinical and Experimental Medicine, Linköping University, SE-58185 Linköping, Sweden. elena.vikstrom@liu.se
Bui
Lan
L
Konradsson
Peter
P
Magnusson
Karl-Eric
KE
D054742:Acyl-Butyrolactones; D001426:Bacterial Proteins / Q000502:physiology*; D015153:Blotting, Western; D018938:Caco-2 Cells; D020013:Calcium Signaling / Q000502:physiology*; D002352:Carrier Proteins / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D010766:Phosphorylation; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D053038:Quorum Sensing / Q000502:physiology*; D011956:Receptors, Cell Surface; D019108:Tight Junctions / Q000378:metabolism / Q000473:pathology*; D062825:Zonula Occludens Proteins
D054742:Acyl-Butyrolactones; D001426:Bacterial Proteins; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; C407243:F11R protein, human; D007136:Immunoglobulins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; C111873:TJP3 protein, human; D062825:Zonula Occludens Proteins