title: Cell saver for on-pump coronary operations reduces systemic inflammatory markers: a randomized trial.
abstract: BACKGROUND
  This study investigated whether intraoperative use of a cell saver reduces the systemic inflammatory response after coronary operations using cardiopulmonary bypass (CPB).
  
  
  METHODS
  The study randomized 29 patients, 15 to cell saving of pericardial suction blood and residual blood in the CPB circuit after perfusion (cell saver group) vs 14 who received direct retransfusion of the suction blood and the CPB circuit blood (control group). Outcome measures were plasma concentrations of the inflammatory markers interleukin (IL)-1beta, IL-6, IL-8, IL-10, IL-12, tumor necrosis factor-alpha, soluble tumor necrosis factor receptors I and II, and procalcitonin at 6, 24, and 72 hours postoperatively.
  
  
  RESULTS
  At 6 hours postoperatively, the cell saver group displayed significantly reduced plasma levels of IL-6 and IL-8 (p < 0.05). A reduction in IL-10 was also found (p = 0.05), along with nonsignificant reductions in the remaining markers. At 24 and 72 hours, significant differences between groups no longer existed. In the cell saver group, the suction blood and CPB circuit blood were cleared for tumor necrosis factor receptors (p < 0.005), and IL-6, IL-8, IL-10, and procalcitonin were significantly reduced (p < 0.05). Median intraoperative blood loss was 250 mL in the cell saver group vs 475 mL (p < 0.02). Immediately postoperatively the hemoglobin level was higher in the cell saver group (p < 0.03). Transfusion requirements were similar.
  
  
  CONCLUSIONS
  The cell saver reduced the systemic levels of the proinflammatory markers IL-6 and IL-8 at 6 hours after CPB. The role of the anti-inflammatory molecules IL-10 and soluble tumor necrosis factor receptors is undefined in this setting.
authors.affiliation: Department of Cardiothoracic Surgery, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark. sunedamgaard@dadlnet.dk
authors.forename: Sune
authors.initials: S
authors.lastname: Damgaard
authors.forename: Claus H
authors.initials: CH
authors.lastname: Nielsen
authors.forename: Lars W
authors.initials: LW
authors.lastname: Andersen
authors.forename: Klaus
authors.initials: K
authors.lastname: Bendtzen
authors.forename: Michael
authors.initials: M
authors.lastname: Tvede
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Steinbrüchel
mesh_terms: D000368:Aged; D001804:Blood Transfusion, Autologous / Q000379:methods*; D002116:Calcitonin / Q000097:blood; D015740:Calcitonin Gene-Related Peptide; D002315:Cardiopulmonary Bypass / Q000379:methods*; D001026:Coronary Artery Bypass / Q000379:methods*; D003327:Coronary Disease / Q000401:mortality / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D007378:Interleukins / Q000097:blood*; D007430:Intraoperative Care / Q000379:methods; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications / Q000097:blood / Q000401:mortality; D011336:Probability; D011446:Prospective Studies; D011498:Protein Precursors / Q000097:blood; D012016:Reference Values; D018570:Risk Assessment; D018709:Statistics, Nonparametric; D016019:Survival Analysis; D015996:Survival Rate; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: C000606589:CALCA protein, human; D018836:Inflammation Mediators; D007378:Interleukins; D011498:Protein Precursors; D014409:Tumor Necrosis Factor-alpha; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide