Subcellular localization of Mitf in monocytic cells.
Microphthalmia-associated transcription factor (Mitf) is a transcription factor that plays an important role in regulating the development of several cell lineages. The subcellular localization of Mitf is dynamic and is associated with its transcription activity. In this study, we examined factors that affect its subcellular localization in cells derived from the monocytic lineage since Mitf is present abundantly in these cells. We identified a domain encoded by Mitf exon 1B1b to be important for Mitf to commute between the cytoplasm and the nucleus. Deletion of this domain disrupts the shuttling of Mitf to the cytoplasm and results in its retention in the nucleus. M-CSF and RANKL both induce nuclear translocation of Mitf. We showed that Mitf nuclear transport is greatly influenced by ratio of M-CSF/Mitf protein expression. In addition, cell attachment to a solid surface also is needed for the nuclear transport of Mitf.
Lu
Ssu-Yi
SY
Department of Diagnostic and Surgical Sciences, School of Dentistry, University of California, Los Angeles, CA 90095, USA.
Wan
Hsiao-Ching
HC
Li
Mengtao
M
Lin
Yi-Ling
YL
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D020033:Protein Isoforms
D051739:Microphthalmia-Associated Transcription Factor; D020033:Protein Isoforms; D016173:Macrophage Colony-Stimulating Factor