Lead-contaminated soil induced oxidative stress, defense response and its indicative biomarkers in roots of Vicia faba seedlings.
Seeds of Vicia faba. L were grown in increasing concentrations of lead (Pb)-added soils (0-2,000 mg/kg). After germination of 25 days, roots were harvested to investigate oxidative stress, defense response and indicative biomarkers based upon chemical analyses and biological measurements. The results showed that higher concentrations of Pb-polluted soils led to seedling growth inhibition, indicative of phytotoxicity. O (2) (*-) and lipid peroxidation were increased with the increase of available Pb in soils and Pb contents in roots, displaying a "J"-shaped dose response curve, whereas H(2)O(2) showed a biphasic dose response curve (a consecutive "J"-shaped and inverted "U"-shaped curve). Superoxide dismutase (SOD), guaiacol peroxidase (POD) and ascorbate peroxidase (APX) enzymes were activated by soil Pb, displaying biphasic curves. The upregulated POD and APX enzymes might be major scavengers of excessive H(2)O(2) when CAT activities were drastically reduced with the increasing soil Pb. The enhanced glutathione (GSH) and APX activities suggested that GSH-ascorbate cycle also participated in eliminating H(2)O(2). Moreover, obvious changes were observed in SOD, CAT and POD isoenzyme patterns, but not in APX except increasing intensities of bands. HSP70 synthesis was significantly induced by extraneous Pb from 125 to 1,000 mg/kg and showed a biphasic curve in this experiment. Comparatively, HSP70 and lipid peroxidation might be more sensitive than other parameters in response to Pb stress, suggesting that these two parameters in the roots might be potential biomarkers for early bioassay of Pb-contaminated soils.
Wang
Chengrun
C
State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, 210093 Nanjing, People's Republic of China. chengrunw66@yahoo.com.cn
Tian
Yuan
Y
Wang
Xiaorong
X
Geng
Jinju
J
Jiang
Jinlin
J
Yu
Hongxia
H
Wang
Chen
C
D015415:Biomarkers / Q000378:metabolism; D004784:Environmental Monitoring; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D007854:Lead / Q000032:analysis / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress; D012639:Seeds / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D012987:Soil; D012989:Soil Pollutants / Q000032:analysis / Q000494:pharmacology*; D031307:Vicia faba / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism
D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D012987:Soil; D012989:Soil Pollutants; D007854:Lead