title: OmpA variants affecting the adherence of ulcerative colitis-derived Bacteroides vulgatus.
abstract: To investigate the adhesion factor of Bacteroides vulgatus derived from ulcerative colitis (UC), we isolated B. vulgatus strains from the large intestinal mucosa of UC patients and non-UC individuals, and examined their adherence to tissue-cultured cells. The adherence to tissue-cultured cells in UC-derived strains (36.5 +/- 7.9 %) was higher than that in non-UC-derived strains (13.2 +/- 7.7 %). PCR and sequencing analysis of outer membrane proteins revealed that the strains derived from five of seven (71.4 %) UC patients had ompA genes belonging to either ompA variant type A or B. The adherence rates in Escherichia coli DH5 a transformants with ompA type A variants (33.3 +/- 4.6 %) and type B variants (34.6 +/- 7.1 %) were higher than the rate in those with non-UC ompA (16.4 +/- 4.0 %). Our results suggest that B. vulgatus isolates in the mucosal flora of the large intestine of UC patients have a high frequency of ompA variants and that the variation of ompA variants is one of the factor causing an increase in the adherence of the bacterium.
authors.affiliation: Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan. ken-mi@tmd.ac.jp
authors.forename: Kenya
authors.initials: K
authors.lastname: Sato
authors.forename: Wakako
authors.initials: W
authors.lastname: Kumita
authors.forename: Takashi
authors.initials: T
authors.lastname: Ode
authors.forename: Shizuko
authors.initials: S
authors.lastname: Ichinose
authors.forename: Akira
authors.initials: A
authors.lastname: Ando
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Fujiyama
authors.forename: Toshio
authors.initials: T
authors.lastname: Chida
authors.forename: Noboru
authors.initials: N
authors.lastname: Okamura
mesh_terms: D001422:Bacterial Adhesion / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000502:physiology; D001439:Bacteroides / Q000235:genetics* / Q000502:physiology; D018938:Caco-2 Cells; D003093:Colitis, Ulcerative / Q000382:microbiology*; D014644:Genetic Variation; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins