title: Actions of melatonin mixed with collagenized porcine bone versus porcine bone only on osteointegration of dental implants.
abstract: This study evaluated the effect of the topical application of melatonin mixed with collagenized porcine bone on the osteointegration on the rough discrete calcium deposit (DCD) surface implants in Beagle dogs 3 months after their insertion. In preparation for subsequent insertion of dental implants, lower molars were extracted from 12 Beagle dogs. Each mandible received two parallel wall expanded platform implants with a DCD surface of 4 mm in diameter and 10 mm in length. The implants were randomly assigned to the distal sites on each mandible in the molar area and the gaps were filled with 5 mg lyophilized powdered melatonin and porcine bone and collagenized porcine bone alone. Ten histological sections per implant were obtained for histomorphometric studies. After a 4-wk treatment period, melatonin plus porcine bone significantly increased the perimeter of bone that was in direct contact with the treated implants (P < 0.0001), bone density (P < 0.0001), and new bone formation (P < 0.0001) in comparison with porcine bone alone around the implants. Melatonin plus collagenized porcine bone on DCD surface may act as a biomimetic agent in the placement of endo-osseous dental implants and enhance the osteointegration. Melatonin combined with porcine bone on DCD implants reveals more bone in implant contact at 12 wk (84.5 +/- 1.5%) compared with porcine bone alone treated area (67.17 +/- 1.2%).
authors.affiliation: Department of General and Implant Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Calvo-Guirado
authors.affiliation: Department of Pharmacological Interactions in Dentistry, Dental School, University of Granada, Granada, Spain.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Gómez-Moreno
authors.affiliation: Department of General and Implant Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: López-Marí
authors.affiliation: Department of Pharmacological Interactions in Dentistry, Dental School, University of Granada, Granada, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Guardia
authors.affiliation: Department of Oral Surgery, Dental School, University Complutense, Madrid, Spain.
authors.forename: José María
authors.initials: JM
authors.lastname: Marínez-González
authors.affiliation: Department of Oral Surgery, Faculty of Medicine, University of Genova, Genova, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Barone
authors.affiliation: Department of Physiology, Faculty of Medicine, University Complutense of Madrid, Madrid, Spain.
authors.forename: Isabel F
authors.initials: IF
authors.lastname: Tresguerres
authors.affiliation: Department of Cellular and Structural Biology, University of Texas, San Antonio, TX, USA.
authors.forename: Sergio D
authors.initials: SD
authors.lastname: Paredes
authors.affiliation: Department of Cellular and Structural Biology, University of Texas, San Antonio, TX, USA.
authors.forename: Lorena
authors.initials: L
authors.lastname: Fuentes-Breto
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001862:Bone Resorption / Q000378:metabolism; D016025:Bone Transplantation / Q000379:methods*; D002118:Calcium / Q000378:metabolism; D003094:Collagen / Q000494:pharmacology*; D015921:Dental Implants; D004285:Dogs; D006651:Histocytochemistry; D008297:Male; D008334:Mandible / Q000378:metabolism / Q000601:surgery; D008550:Melatonin / Q000494:pharmacology*; D016348:Osseointegration / Q000187:drug effects*; D010010:Osteoclasts / Q000378:metabolism; D011861:Radiography, Dental; D013552:Swine
chemical_list: D015921:Dental Implants; D003094:Collagen; D008550:Melatonin; D002118:Calcium