The DL1 repeats in the genome of Diphyllobothrium latum.
Diphyllobothrium latum is a widespread intestinal parasite, which has a great clinical relevance, but there are no sequences of its nuclear genome. In this paper, a repetitive element in the D. latum genome is firstly described. The adult D. latum was obtained in the result of expulsion from intestinum of a patient suffering from diphyllobothriasis. Genomic DNA was isolated from several proglottids of this individual. PstI restriction products of D. latum genomic DNA were sequenced. Polymerase chain reaction (PCR) amplification of these products using genomic DNA and selected primers was carried out. Thereby a cluster of a repetitive element, called DL1, was discovered. For precise identification of a beginning and an end of the repeat, a product of PCR amplification of D. latum genomic DNA with one specific primer was sequenced. In discussion, several evidences that DL1 repeat is a member of the SINE family of retroposons were adduced.
Usmanova
Nadezhda M
NM
Russian Academy of Sciences, Institute of Cytology, Tikchoretskii Avenue 4, 194064, St. Petersburg, Russia. nmusmanova@gmail.com
Kazakov
Vasiliy I
VI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics*; D004169:Diphyllobothriasis / Q000188:drug therapy / Q000469:parasitology*; D004170:Diphyllobothrium / Q000235:genetics* / Q000302:isolation & purification; D049751:Genome, Helminth; D006801:Humans; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018509:DNA, Helminth