A semi-quantitative FIA-ESI-MS method for the rapid screening of Hypericum perforatum crude extracts.
A method based on FIA-ESI-MS has been developed to profile the major constituents of Hypericum perforatum extracts. The objective was to obtain simultaneous semi-quantitative data on hypericin, chlorogenic acid, rutin, quercitrin, quercetin and hyperforin contents for a high-throughput screening of the raw plant material. The principal drawback of FIA-ESI analysis of complex mixtures involves ion suppression effects: the ionization of some components of the mixture can be severely suppressed by ionization of others. The results show that this problem can be alleviated using a new approach to generate calibration curves.
Piovan
Anna
A
Department of Biology, University of Padua, Via U. Bassi 58/B - 35131 Padova, Italy.
Filippini
Raffaella
R
Caniato
Rosy
R
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D020902:Hypericum / Q000737:chemistry*; D007202:Indicators and Reagents; D010936:Plant Extracts / Q000737:chemistry; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D010936:Plant Extracts