Species-specific type II restriction-modification system of Xylella fastidiosa temecula1.
The transformation efficiency of Xylella fastidiosa can be increased by interfering with restriction by the strain-specific type II system encoded by the PD1607 and PD1608 genes. Here, we report results for two strategies: in vitro methylation using M.SssI and isolation of DNA from an Escherichia coli strain expressing the methylase PD1607.
Matsumoto
Ayumi
A
Department of Microbiology, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
Igo
Michele M
MM
D004247:DNA / Q000378:metabolism; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics* / Q000378:metabolism*; D018014:Gene Transfer Techniques; D008745:Methylation; D014169:Transformation, Bacterial; D044167:Xylella / Q000201:enzymology*
D015280:DNA Restriction-Modification Enzymes; D004247:DNA