Identification of differential proteins in colon cancer SW480 cells with HIF1-alpha silence by proteome analysis.
Hypoxia is one of the basic characteristics of the colorectal cancer and HIF1 plays a central role in tumor hypoxia adaptation and controls the expression of a variety of genes. But it had not reached a comprehensive understanding of them in colorectal cancer in hypoxia. This study silenced HIF1-alpha, which is the regulatory subunit of HIF1, and established two dimensional gel electrophoresis profiles for human SW480siHIF1-alpha and SW480N cells to identified proteins with differential expressions. Ten down-regulated proteins and nine up-regulated proteins were identified by HPLC-Chip-MS/MS. Down-regulated proteins including PLD2, ANXA2. HIF1-alpha had a clear positive correlation with PLD2 and ANXA2 in colon adenocarcinoma samples. There proteins might have a direct or indirect contact HIF1-alpha, and play a critical role in apoptosis, signal transduction and hypoxia adaptation.
Wu
X Y
XY
Department of Gastrointestinal Surgery, First Affiliated Hosptial of Chongqing Medical University, Chongqing, China.
Fu
Z X
ZX
Wang
X H
XH
Shen
W
W
D000230:Adenocarcinoma / Q000737:chemistry* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D020868:Gene Silencing / Q000502:physiology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D009363:Neoplasm Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009363:Neoplasm Proteins; D020543:Proteome; D012333:RNA, Messenger