Detection of Trypanosoma cruzi and T. rangeli infections from Rhodnius pallescens bugs by loop-mediated isothermal amplification (LAMP).
We have developed two loop-mediated isothermal amplification (LAMP) assays for specific detection of Trypanosoma cruzi and Trypanosoma rangeli based on the 18S ribosomal RNA (rRNA) and the small nucleolar RNA (snoRNA) genes, respectively. The detection limit of the assays is 100 fg and 1 pg for T. cruzi and T. rangeli, respectively, with reactions conducted in 60 minutes. The two LAMP assays were used in detection of T. cruzi and T. rangeli infections in comparison with polymerase chain reaction (PCR) for DNA samples extracted from Rhodnius pallescens bugs collected from palm trees in Panama. Out of a total of 52 DNA samples from R. pallescens bugs 17 (33%) and 14 (27%) were T. cruzi-positive by LAMP and PCR, respectively, while, 7 (13%) and 4 (8%) were T. rangeli-positive by LAMP and PCR, respectively. Further evaluation of these LAMP assays is needed, especially with specimens collected from human patients as well as blood kept for transfusion purposes.
Thekisoe
Oriel M M
OM
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Rodriguez
Carol V
CV
Rivas
Francisco
F
Coronel-Servian
Andrea M
AM
Fukumoto
Shinya
S
Sugimoto
Chihiro
C
Kawazu
Shin-Ichiro
S
Inoue
Noboru
N
D000818:Animals; D016054:DNA, Protozoan / Q000302:isolation & purification; D007303:Insect Vectors / Q000469:parasitology*; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D012239:Rhodnius / Q000469:parasitology*; D013047:Specific Pathogen-Free Organisms; D014345:Trypanosoma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014352:Trypanosomiasis / Q000635:transmission*
D016054:DNA, Protozoan