Quantitative analysis of mRNA for 10 different drug resistance factors in dogs with lymphoma.
Expression levels of ABCB1, ABCC1, Lung resistance-associated protein (LRP), ABCG2, p53, p21(waf1), Bcl-2, CD40L, glutathione S-transferase alpha (GSTα), and O⁶-methylguanine-DNA-methyltransferase (MGMT) genes, and mutation of p53 gene were examined in 23 dogs with multicentric high-grade lymphoma to explore their association with drug resistance of the tumor cells. Dogs were divided into chemotherapy-sensitive (n=13) and -resistant (n=10) groups according to the response to a 6-month modified version of the University of Wisconsin (UW)-Madison chemotherapy protocol (UW-25), and expression levels of these factors and frequency of p53 gene mutation were compared between groups. No significant differences were observed in expression levels of each factor between groups. However, 4 dogs in the chemotherapy-resistant group showed high expression of ABCB1. No significant difference was observed in the frequency of p53 mutation between groups. A possible association of ABCB1 with resistance to UW-25 was shown, but no uniform mechanism associated with drug resistance could be identified in dogs with lymphoma.
Tomiyasu
Hirotaka
H
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113–8657, Japan. atsuji@mail.ecc.u-tokyo.ac.jp
Goto-Koshino
Yuko
Y
Takahashi
Masashi
M
Fujino
Yasuhito
Y
Ohno
Koichi
K
Tsujimoto
Hajime
H
D000818:Animals; D017931:DNA Primers; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D016158:Genes, p53; D008223:Lymphoma / Q000235:genetics / Q000662:veterinary*; D008297:Male; D009154:Mutation; D009919:Orchiectomy / Q000662:veterinary; D010052:Ovariectomy / Q000662:veterinary; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D012333:RNA, Messenger