title: [RH hybrid box identification in subjects with Rh negative phenotype from Mexico's Valley].
abstract: BACKGROUND
  The prevalence of the RhD and RhCE gene alleles is related to the ethnic mixture. The aim of this report is to describe the predominant molecular mechanisms in RhD negative subjects residents from Mexico's valley according to the phenotype of RhCE.
  
  
  METHODS
  Blood samples from RhD negative women and men were studied. The RhD/RhCE phenotype was identified by hemagglutination and Rh hybrid box by PCR-FRLP with PstI.
  
  
  RESULTS
  216 subjects were included. The RhD phenotypes were ccdee in 179 cases (82.8%), Ccdee in 15 cases (11.6%), ccdEe in seven (3.2%), CcdEe in four (1.9%), and CcdEE in a single subject (0.5%). In five cases, RhD hybrid box was not amplified (2.3%), 21 cases were hemizygotes (9.7%), and 188 cases homozygotes (87%), for RhD hybrid box. The homozygote condition was more frequent in those individuals with phenotype ccdee (87%). The allelic frequency of RhD hybrid box was 0.928. The frequency of Rhcc haplotype was higher in those subjects homozygotes for RhD hybrid box (chi2 = 4.658, p < 0.05).
  
  
  CONCLUSIONS
  In this population, RhD gene deletion is the main molecular mechanism to generate to RhD negative condition and this depends on the European mixture.
authors.affiliation: Coordinación de Hematología Perinatal, Subdirección de Investigación Clínica, Instituto Nacional de Perinatología, SSA, Medicina Transfusional y Banco de Sangre, Fundación Clínica Médica Sur, México D.F., México. baptista@infosel.net.mx
authors.forename: Héctor Alfredo
authors.initials: HA
authors.lastname: Baptista-González
authors.forename: Fany
authors.initials: F
authors.lastname: Rosenfeld-Mann
authors.forename: Rocío
authors.initials: R
authors.lastname: Trueba-Gómez
authors.forename: Elba
authors.initials: E
authors.lastname: Reyes-Maldonado
authors.forename: Luis A
authors.initials: LA
authors.lastname: Jiménez-Zamudio
mesh_terms: D005260:Female; D006801:Humans; D008297:Male; D008800:Mexico; D012204:Rh-Hr Blood-Group System / Q000235:genetics*
chemical_list: D012204:Rh-Hr Blood-Group System