Actinoallomurus oryzae sp. nov., an endophytic actinomycete isolated from roots of a Thai jasmine rice plant.
The novel strain GMKU 370(T) was isolated from roots of a Thai jasmine rice plant (Oryza sativa L. 'KDML 105') collected from Pathum Thani Rice Research Center, Pathum Thani province, Thailand. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GMKU 370(T) formed a distinct clade within the genus Actinoallomurus. Strain GMKU 370(T) contained meso-diaminopimelic acid and lysine in the cell-wall peptidoglycan and galactose and madurose as whole-cell sugars. No mycolic acids were detected. The predominant menaquinones were MK-9(H(6)) and MK-9(H(8)). The polar phospholipids consisted of phosphatidylglycerol and phosphatidylinositol. These chemical properties reveal that strain GMKU 370(T) belongs to the genus Actinoallomurus. Strain GMKU 370(T) is distinct from the phylogenetically closely related type strains Actinoallomurus iriomotensis NBRC 103685(T) and Actinoallomurus coprocola NBRC 103688(T) (99.20 % 16S rRNA gene sequence similarity to both) in terms of differences in phenotypic characteristics and DNA-DNA relatedness (51 and 17 %, respectively). It is proposed that strain GMKU 370(T) represents a novel species of the genus Actinoallomurus, for which the name Actinoallomurus oryzae sp. nov. is proposed. The type strain is GMKU 370(T) ( = BCC 31373(T)  = NBRC 105246(T)).
Indananda
Chantra
C
Department of Genetics, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Thamchaipenet
Arinthip
A
Department of Genetics, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Matsumoto
Atsuko
A
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Inahashi
Yuki
Y
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Duangmal
Kannika
K
Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Takahashi
Yoko
Y
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000382:microbiology*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013785:Thailand; D024482:Vitamin K 2 / Q000032:analysis
D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid