Improvement of the representation of bifidobacteria in fecal microbiota metagenomic libraries by application of the cpn60 universal primer cocktail.
Actinobacteria, particularly bifidobacteria, are widely observed to be underrepresented in metagenomic studies of microbial communities. We have compared human fecal microbiota clone libraries based on 16S rRNA and cpn60 PCR products. Taxonomic profiles were similar except that the cpn60 libraries contained large numbers of bifidobacterial sequences.
Hill
Janet E
JE
University of Saskatchewan, 52 Campus Drive, Saskatoon, SK, Canada S7N 5B4. Janet.Hill@usask.ca
Fernando
W M Ursla
WM
Zello
Gordon A
GA
Tyler
Robert T
RT
Dahl
Wendy J
WJ
Van Kessel
Andrew G
AG
D000293:Adolescent; D000328:Adult; D000368:Aged; D001644:Bifidobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D018834:Chaperonin 60 / Q000235:genetics*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D015723:Gene Library; D006801:Humans; D056186:Metagenomics; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D055815:Young Adult
D018834:Chaperonin 60; D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S