A simple approach to patterned protein immobilization on silicon via electrografting from diazonium salt solutions.
A highly versatile method utilizing diazonium salt chemistry has been developed for the fabrication of protein arrays. Conventional ultraviolet mask lithography was used to pattern micrometer sized regions into a commercial photoresist on a highly doped p-type silicon (100) substrate. These patterned regions were used as a template for the electrochemical grafting of the in situ generated p-aminobenzenediazonium cation to form patterns of aminophenyl film on silicon. Immobilization of biomolecules was demonstrated by coupling biotin to the aminophenyl regions followed by reaction with fluorescently labeled avidin and visualization with fluorescence microscopy. This simple patterning strategy is promising for future application in biosensor devices.
Flavel
Benjamin S
BS
Department of Chemistry, University of Canterbury, Christchurch 8140, New Zealand. ben.flavel@flinders.edu.au
Gross
Andrew J
AJ
Garrett
David J
DJ
Nock
Volker
V
Downard
Alison J
AJ
D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D002412:Cations; D003979:Diazonium Compounds / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D008027:Light; D018625:Microscopy, Atomic Force / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D011506:Proteins / Q000737:chemistry*; D012492:Salts / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*
D002412:Cations; D003979:Diazonium Compounds; D011506:Proteins; D012492:Salts; D001360:Avidin; C028214:4-dimethylaminobenzenediazonium fluoroborate; D001710:Biotin; D012825:Silicon