[Effects of Selaginella doederleinii on human nasopharyngeal carcinoma TW03 cells in vitro and its mechanism].
OBJECTIVE
To study the inhibition and mechanism of Selaginella doederleinii (SLG) on the growth of nasopharyngeal carcinoma (NPC) TW03 cells in vitro.


METHODS
Cell suppression rates were measured by MTT assay, cell cycles were measured by flow cytometry (FCM) and expressions of Bc1-2 and Bax protein were detected by immunohistochemistry.


RESULTS
Inhibition on the growth of NPC cell was intensed by SLG in a dose and time dependent manner in vitro (P < 0.05). The ratio of S phase cells increased and the ratios of G0/G1 and G2/M phase cells decreased, with the addition of SLG. Meanwhile, the expression of Bcl-2 protein was down-regulated and Bax protein was up-regulated.


CONCLUSIONS
SLG could inhibit the growth of human NPC TW03 cells by directing cell cycle arrested at S phase, down-regulating Bcl-2 protein expression and up-regulating Bax protein expression.
Jing
Yan
Y
First Hospital Affiliated of Guangxi Medical University, Nanning 530021, China. jmywh@126.com
Tang
An-zhou
AZ
Liu
Jin
J
Kang
Min
M
Deng
Peng
P
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D010946:Plants, Medicinal / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D032503:Selaginellaceae / Q000737:chemistry*; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein