Quantitation of virus using laser-based scanning of near-infrared fluorophores replaces manual plate reading in a virus titration assay.
A method was developed for quantitation of a virus titration assay for minimally cytopathic and noncytopathic viruses that utilizes laser-based scanning of near-infrared (NIR) fluorophores. This automated method bypasses the need for manual plate reading thus eliminating human bias and error. The image data is translated by LI-COR's Odyssey software into numerical data which is used directly in the virus titer calculations.
Weldon
Sally K
SK
LI-COR Biosciences, Lincoln, NE 68504, USA. sally.weldon@licor.com
Mischnick
Shawn L
SL
Urlacher
Teresa M
TM
Ambroz
Kristi L H
KL
D000818:Animals; D001331:Automation / Q000379:methods*; D002460:Cell Line; D028301:Feline Panleukopenia Virus / Q000302:isolation & purification*; D007834:Lasers; D019562:Viral Load / Q000379:methods*