title: Nano-TiO 2 enriched polymeric powder coatings support human mesenchymal cell attachment and growth.
abstract: The objective of this study was to utilize ultrafine powder coating technology to prepare (PPC) that can support human mesenchymal cell attachment and growth. Resins were modified with titanium dioxide and polytetrafluoroethylene (PTFE), and enriched with either SiO(2) or TiO(2) nanoparticles (nSiO(2) or nTiO(2)) to create continuous PPC. Scanning electron microscopy (SEM) revealed complex surface topographies with nano features, and energy dispersive X-ray (EDX) analysis with Ti mapping confirmed a homogenous dispersion of the material. SEM and inverted fluorescence microscopy showed that human embryonic palatal mesenchymal (HEPM) cells attached and spread out on the PPC surfaces, particularly those enriched with nTiO( 2). Cell counts were higher, and the MTT assay measured more metabolic activity from the nTiO(2) enriched PPCs. Furthermore, these cellular responses were enhanced on PPC surfaces that were enriched with a higher concentration of nTiO(2) (2% vs. 0.5%), and appeared comparable to that seen on commercially pure titanium (cpTi). Therefore the nTiO( 2) enrichment of PPC was shown to favor human mesenchymal cell attachment and growth. Indeed, this modification of the materials created continuous surface coatings that sustained a favorable cellular response.
authors.affiliation: Department of Chemical and Biochemical Engineering University of Western Ontario, London, Canada.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Sayem Mozumder
authors.forename: Jesse
authors.initials: J
authors.lastname: Zhu
authors.forename: Hiran
authors.initials: H
authors.lastname: Perinpanayagam
mesh_terms: D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D011138:Polytetrafluoroethylene / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; C009495:titanium dioxide; D011138:Polytetrafluoroethylene; D014025:Titanium