The utility of discovered on gastrointestinal stromal tumor 1 (DOG1) antibody in surgical pathology-the GIST of it.
DOG1 (discovered on GIST 1), known also as TMEM16A and ANO1, has emerged in recent years as a promising biomarker for gastrointestinal stromal tumors (GIST). It was originally discovered through microarray expression profiling analysis as gene that is highly expressed in GIST, and subsequent immunohistochemical studies have shown its use in its diagnosis. The results from several series have shown a high overall sensitivity and specificity for DOG1 in the detection of GISTs and about 6% of GISTs overall exhibiting a DOG1+/KIT-immunoprofile. DOG1 antibodies are more sensitive than KIT antibodies in detecting tumors of gastric origin, tumors with epithelioid morphology, and tumors harboring PDGFRA mutation. Furthermore, DOG1 immunoreactivity is rarely observed in other mesenchymal and nonmesenchymal tumor types. These results support the use of DOG1 as a diagnostic biomarker for GIST. When used in combination with KIT, this panel of diagnostic biomarkers can help pathologists and clinicians to identify more patients who may benefit from targeted therapies.
Lee
Cheng-Han
CH
Department of Pathology and Laboratory Medicine, Vancouver General Hospital, University of British Columbia, Canada. chenghan@interchange.ubc.ca
Liang
Cher-wei
CW
Espinosa
Inigo
I
D000075369:Anoctamin-1; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis; D018118:Chloride Channels; D046152:Gastrointestinal Stromal Tumors / Q000175:diagnosis* / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism*
C476213:ANO1 protein, human; D000075369:Anoctamin-1; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D018118:Chloride Channels; D008565:Membrane Proteins; D009363:Neoplasm Proteins