Protection against Helicobacter pylori infection by a trivalent fusion vaccine based on a fragment of urease B-UreB414.
A multivalent fusion vaccine is a promising option for protection against Helicobacter pylori infection. In this study, UreB414 was identified as an antigenic fragment of urease B subunit (UreB) and it induced an antibody inhibiting urease activity. Immunization with UreB414 partially protected mice from H. pylori infection. Furthermore, a trivalent fusion vaccine was constructed by genetically linking heat shock protein A (HspA), H. pylori adhesin A (HpaA), and UreB414, resulting in recombinant HspA-HpaA-UreB414 (rHHU). Its protective effect against H. pylori infection was tested in BALB/c mice. Oral administration of rHHU significantly protected mice from H. pylori infection, which was associated with H. pylori-specific antibody production and Th1/Th2-type immune responses. The results show that a trivalent fusion vaccine efficiently combats H. pylori infection, and that an antigenic fragment of the protein can be used instead of the whole protein to construct a multivalent vaccine.
Wang
Li
L
Department of Pharmacy, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032, PR China.
Liu
Xiao-Fei
XF
Yun
Shi
S
Yuan
Xiao-Peng
XP
Mao
Xu-Hu
XH
Wu
Chao
C
Zhang
Wei-Jun
WJ
Liu
Kai-Yun
KY
Guo
Gang
G
Lu
Dong-Shui
DS
Tong
Wen-De
WD
Wen
Ai-Dong
AD
Zou
Quan-Ming
QM
D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology*; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005260:Female; D006360:Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D016481:Helicobacter Infections / Q000276:immunology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013270:Stomach / Q000382:microbiology / Q000473:pathology; D014510:Urease / Q000235:genetics / Q000276:immunology*; D037521:Virulence Factors / Q000235:genetics / Q000276:immunology
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins; C113725:HspA protein, bacteria; D011993:Recombinant Fusion Proteins; D037521:Virulence Factors; C095977:adhesin A, Helicobacter; D015847:Interleukin-4; D007371:Interferon-gamma; D014510:Urease