Hantavirus-like particles generated in CHO cells induce specific immune responses in C57BL/6 mice.
A safe and effective hantavirus vaccine is highly desirable since hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, HFRS). Virus-like particles (VLPs) displaying functional viral proteins could provide effective vaccines against a few viruses, but their ability to induce hantavirus-specific immune response has not been adequately investigated. To measure the immunogenicity of Hantaan virus-like particles (HTN-VLPs) vaccine, we generated recombinant HTN-VLPs by co-expressing Hantaan virus nucleocapsid (N) protein and glycoproteins (Gn and Gc) in Chinese hamster ovary (CHO) cells. We compared intramuscular versus subcutaneous administration of HTN-VLPs for the ability to induce specific immune response against Hantaan virus infection. Mice that received both intramuscular and subcutaneous immunizations of HTN-VLPs were sufficiently stimulated specific antibody response against Hantaan virus N protein and glycoproteins, which was comparable to Chinese commercial inactivated bivalent hantaviruses vaccine. Moreover, vaccination with HTN-VLPs also resulted in the induction of higher levels of specific cellular response to N protein than that of inactivated vaccine. Our results provide an important insight towards the development of hantaviruses-like particles as a potential candidate vaccine for the control and prevention of hantaviruses infection.
Li
Chuan
C
State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC 100 Ying Xin Jie, Xuan Wu Qu, Beijing 100052, China.
Liu
Feng
F
Liang
Mifang
M
Zhang
Quanfu
Q
Wang
Xiaofang
X
Wang
Tao
T
Li
Jiandong
J
Li
Dexin
D
D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016466:CHO Cells; D036022:Capsid Proteins / Q000276:immunology; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D018063:Hantaan virus / Q000254:growth & development / Q000276:immunology*; D018778:Hantavirus Infections / Q000276:immunology / Q000517:prevention & control*; D007074:Immunoglobulin G / Q000097:blood; D007273:Injections, Intramuscular; D007279:Injections, Subcutaneous; D051379:Mice; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D014709:Vero Cells; D014758:Viral Core Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D014771:Virion / Q000254:growth & development* / Q000276:immunology
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; D014758:Viral Core Proteins; D014765:Viral Vaccines; C111782:nucleocapsid protein, Hantaan virus