Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and proteins.
We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomaterials for small analytes (e.g., glutathione); Pt nanosponges and Fe(3)O(4) NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomaterials have several advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations (fewer problems associated with "sweet spots"), when used in laser desorption/ionization mass spectrometry.
Chiang
Cheng-Kang
CK
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Chiang
Ni-Chen
NC
Lin
Zong-Hong
ZH
Lan
Guo-Yu
GY
Lin
Yang-Wei
YW
Chang
Huan-Tsung
HT
D045304:Cytochromes c / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D016014:Linear Models; D053768:Metal Nanoparticles / Q000737:chemistry; D008970:Molecular Weight; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D010455:Peptides; D011506:Proteins; D010984:Platinum; D006046:Gold; D045304:Cytochromes c; D052203:Ferrosoferric Oxide