title: Striking species difference in the contribution of concentrative nucleoside transporter 2 to nucleoside uptake between mouse and rat hepatocytes.
abstract: Concentrative nucleoside transporter 2 (CNT2) (encoded by the SLC28A2 gene) transports various antiviral or antitumor purine nucleoside analogs to be involved in their pharmacokinetics and pharmacological actions. The results of our study showed that mouse hepatocytes hardly expressed CNT2 mRNA and no CNT2-dependent nucleoside uptake was observed, while rat hepatocytes exhibited high CNT2-dependent nucleoside uptake activity levels with abundant CNT2 mRNA expression. We concluded that CNT2 contributes considerably to nucleoside uptake in rat hepatocytes but not in mouse hepatocytes.
authors.affiliation: Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuou-ku, Chiba-shi, Chiba, Japan 260-8675. tomomif@p.chiba-u.ac.jp
authors.forename: Tomomi
authors.initials: T
authors.lastname: Furihata
authors.forename: Yukina
authors.initials: Y
authors.lastname: Fukuchi
authors.forename: Minami
authors.initials: M
authors.lastname: Iikura
authors.forename: Misato
authors.initials: M
authors.lastname: Hashizume
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyajima
authors.forename: Miki
authors.initials: M
authors.lastname: Nagai
authors.forename: Kan
authors.initials: K
authors.lastname: Chiba
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D022781:Hepatocytes / Q000378:metabolism*; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009705:Nucleosides / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012254:Ribavirin / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
chemical_list: D026901:Membrane Transport Proteins; D009705:Nucleosides; C065171:cif nucleoside transporter; D012254:Ribavirin; D012964:Sodium; D000241:Adenosine