title: Metabolic imaging in the anesthetized rat brain using hyperpolarized [1-13C] pyruvate and [1-13C] ethyl pyruvate.
abstract: Formulation, polarization, and dissolution conditions were developed to obtain a stable hyperpolarized solution of [1-(13)C]-ethyl pyruvate. A maximum tolerated concentration and injection rate were determined, and (13)C spectroscopic imaging was used to compare the uptake of hyperpolarized [1-(13)C]-ethyl pyruvate relative to hyperpolarized [1-(13)C]-pyruvate into anesthetized rat brain. Hyperpolarized [1-(13)C]-ethyl pyruvate and [1-(13)C]-pyruvate metabolic imaging in normal brain is demonstrated and quantified in this feasibility and range-finding study.
authors.affiliation: Global Applied Science Laboratory, GE Healthcare, Menlo Park, California 94025, USA. ralph.hurd@ge.com
authors.forename: Ralph E
authors.initials: RE
authors.lastname: Hurd
authors.forename: Yi-Fen
authors.initials: YF
authors.lastname: Yen
authors.forename: Dirk
authors.initials: D
authors.lastname: Mayer
authors.forename: Albert
authors.initials: A
authors.lastname: Chen
authors.forename: David
authors.initials: D
authors.lastname: Wilson
authors.forename: Susan
authors.initials: S
authors.lastname: Kohler
authors.forename: Robert
authors.initials: R
authors.lastname: Bok
authors.forename: Daniel
authors.initials: D
authors.lastname: Vigneron
authors.forename: John
authors.initials: J
authors.lastname: Kurhanewicz
authors.forename: James
authors.initials: J
authors.lastname: Tropp
authors.forename: Daniel
authors.initials: D
authors.lastname: Spielman
authors.forename: Adolf
authors.initials: A
authors.lastname: Pfefferbaum
mesh_terms: D018681:Anesthetics, General / Q000008:administration & dosage*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002247:Carbon Isotopes / Q000493:pharmacokinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D011773:Pyruvates / Q000493:pharmacokinetics*; D019289:Pyruvic Acid / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D018681:Anesthetics, General; D002247:Carbon Isotopes; D011773:Pyruvates; C046522:ethyl pyruvate; D019289:Pyruvic Acid