Natural selection of human embryos: decidualizing endometrial stromal cells serve as sensors of embryo quality upon implantation.
BACKGROUND
Pregnancy is widely viewed as dependent upon an intimate dialogue, mediated by locally secreted factors between a developmentally competent embryo and a receptive endometrium. Reproductive success in humans is however limited, largely because of the high prevalence of chromosomally abnormal preimplantation embryos. Moreover, the transient period of endometrial receptivity in humans uniquely coincides with differentiation of endometrial stromal cells (ESCs) into highly specialized decidual cells, which in the absence of pregnancy invariably triggers menstruation. The role of cyclic decidualization of the endometrium in the implantation process and the nature of the decidual cytokines and growth factors that mediate the crosstalk with the embryo are unknown.


RESULTS
We employed a human co-culture model, consisting of decidualizing ESCs and single hatched blastocysts, to identify the soluble factors involved in implantation. Over the 3-day co-culture period, approximately 75% of embryos arrested whereas the remainder showed normal development. The levels of 14 implantation factors secreted by the stromal cells were determined by multiplex immunoassay. Surprisingly, the presence of a developing embryo had no significant effect on decidual secretions, apart from a modest reduction in IL-5 levels. In contrast, arresting embryos triggered a strong response, characterized by selective inhibition of IL-1beta, -6, -10, -17, -18, eotaxin, and HB-EGF secretion. Co-cultures were repeated with undifferentiated ESCs but none of the secreted cytokines were affected by the presence of a developing or arresting embryo.


CONCLUSIONS
Human ESCs become biosensors of embryo quality upon differentiation into decidual cells. In view of the high incidence of gross chromosomal errors in human preimplantation embryos, cyclic decidualization followed by menstrual shedding may represent a mechanism of natural embryo selection that limits maternal investment in developmentally impaired pregnancies.
Teklenburg
Gijs
G
Department of Reproductive Medicine and Gynecology, University Medical Center Utrecht, Utrecht, The Netherlands.
Salker
Madhuri
M
Molokhia
Mariam
M
Lavery
Stuart
S
Trew
Geoffrey
G
Aojanepong
Tepchongchit
T
Mardon
Helen J
HJ
Lokugamage
Amali U
AU
Rai
Raj
R
Landles
Christian
C
Roelen
Bernard A J
BA
Quenby
Siobhan
S
Kuijk
Ewart W
EW
Kavelaars
Annemieke
A
Heijnen
Cobi J
CJ
Regan
Lesley
L
Brosens
Jan J
JJ
Macklon
Nick S
NS
D015374:Biosensing Techniques; D018920:Coculture Techniques; D010064:Embryo Implantation; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000166:cytology; D004717:Endometrium / Q000166:cytology*; D005260:Female; D056084:Genetic Fitness; D006801:Humans; D011247:Pregnancy; D012641:Selection, Genetic; D017154:Stromal Cells / Q000166:cytology*