Gene expression in proliferating cells of the dinoflagellate Alexandrium catenella (Dinophyceae).
Understanding the conditions leading to harmful algal blooms, especially those produced by toxic dinoflagellate species, is important for environmental and health safety. In addition to investigations into the environmental conditions necessary for the formation of toxic blooms, we postulate that investigating gene expression in proliferating cells is essential for understanding bloom dynamics. Expressed sequence tags were produced from cultured cells of the toxic dinoflagellate Alexandrium catenella sampled during the initiation phase of growth using Sanger's method and by 454 pyrosequencing. A significant proportion of identified genes (ca. 25%) represented enzymes and proteins that participate in a variety of cellular regulatory mechanisms that may characterize proliferating cells, e.g., control of the cell cycle and division, regulation of transcription, translation and posttranslational protein modifications, signaling, intracellular trafficking, and transport. All of the several genes selected for gene expression assays due to their involvement in metabolism and the cell cycle were overexpressed during exponential growth. These data will be useful for investigating the mechanisms underlying growth and toxin production in toxic Alexandrium species and for studying and monitoring the development of toxic blooms.
Toulza
Eve
E
Laboratoire Ecosystèmes Lagunaires (UMR 5119 CNRS-UM2-IFREMER-IRD), Université Montpellier 2, CC 093, Place Eugène Bataillon, 34095 Montpellier Cedex 05, France.
Shin
Mi-Sun
MS
Blanc
Guillaume
G
Audic
Stéphane
S
Laabir
Mohamed
M
Collos
Yves
Y
Claverie
Jean-Michel
JM
Grzebyk
Daniel
D
D049109:Cell Proliferation; D019295:Computational Biology; D004141:Dinoflagellida / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005786:Gene Expression Regulation; D015723:Gene Library; D017125:Genes, Protozoan / Q000235:genetics; D010788:Photosynthesis; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D016053:RNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
D015800:Protozoan Proteins; D016053:RNA, Protozoan