title: Identification of Porphyromonas levii isolated from clinical cases of bovine interdigital necrobacillosis by 16S rRNA sequencing.
abstract: Laboratories use pigmentation, antibiotic susceptibility, and biochemical tests to identify anaerobic organisms that play a role in bovine interdigital necrobacillosis (bovine foot rot). In this study, 16S rRNA gene sequencing was used to identify strains to the species level that were originally classified as Prevotella or Porphyromonas spp by conventional phenotype assessment methods. Of 264 qualified strains from ceftiofur clinical trials, 241 isolates were definitively identified by 16S rRNA sequencing as Porphyromonas levii. Similarly, of 275 qualified strains from tulathromycin clinical trials, 156 isolates were definitively identified by 16S rRNA sequencing as P. levii. The predominance of P. levii in this study supports the role of this organism as an associative agent of bovine foot rot and may have implications for routine laboratory diagnosis.
authors.affiliation: Pfizer Animal Health, Kalamazoo, MI 49007, USA.
authors.forename: M
authors.initials: M
authors.lastname: Sweeney
authors.forename: J
authors.initials: J
authors.lastname: Watts
authors.forename: E
authors.initials: E
authors.lastname: Portis
authors.forename: M
authors.initials: M
authors.lastname: Lucas
authors.forename: R
authors.initials: R
authors.lastname: Nutsch
authors.forename: D
authors.initials: D
authors.lastname: Meeuwse
authors.forename: D
authors.initials: D
authors.lastname: Bade
authors.forename: V
authors.initials: V
authors.lastname: Oliver
authors.forename: D W
authors.initials: DW
authors.lastname: Morck
authors.forename: D
authors.initials: D
authors.lastname: Shinabarger
authors.forename: S
authors.initials: S
authors.lastname: Poppe
authors.forename: M
authors.initials: M
authors.lastname: Peterson
authors.forename: D
authors.initials: D
authors.lastname: Sweeney
authors.forename: M
authors.initials: M
authors.lastname: Knechtel
authors.forename: G
authors.initials: G
authors.lastname: Zurenko
mesh_terms: D000818:Animals; D016866:Bacteroidaceae Infections / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D005534:Foot Diseases / Q000382:microbiology / Q000662:veterinary*; D017861:Porphyromonas / Q000235:genetics* / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D012336:RNA, Ribosomal, 16S