Analysis of adulterated herbal medicines and dietary supplements marketed for weight loss by DOSY 1H-NMR.
Twenty herbal medicines or dietary supplements marketed as natural slimming products were analysed by diffusion ordered spectroscopy (DOSY) 1H-nuclear magnetic resonance (NMR) and DOSY-COSY 1H-NMR. The method allows analysis of the whole sample with the detection of both active and inactive ingredients in these complex matrices. Among the 20 formulations analysed, two were strictly herbal and four had a composition corresponding to declared ingredients on the packaging or the leaflet. The others were all adulterated. Eight formulations contain sibutramine alone at doses ranging from 4.4 to 30.5 mg/capsule. Five formulations contain sibutramine (from 5.0 to 19.6 mg/capsule or tablet) in combination with phenolphthalein (from 4.4 to 66.1 mg/capsule), and the last formulation was adulterated with synephrine (19.5 mg/capsule). Quantification of the actives was carried out with 1H-NMR. Several other compounds were also characterized including methylsynephrine, vitaberin, sugars, vitamins, etc. DOSY NMR is thus proposed as a useful tool for detection of unexpected adulteration.
Vaysse
J
J
Université de Toulouse, UPS, Laboratoire de Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique (SPCMIB), Groupe de RMN Biomédicale, Toulouse, France.
Balayssac
S
S
Gilard
V
V
Desoubdzanne
D
D
Malet-Martino
M
M
Martino
R
R
D019440:Anti-Obesity Agents / Q000032:analysis* / Q000737:chemistry*; D003503:Cyclobutanes / Q000032:analysis / Q000737:chemistry; D019587:Dietary Supplements / Q000032:analysis*; D004340:Drug Contamination; D004335:Drug and Narcotic Control / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005506:Food Contamination; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010592:Pharmaceutic Aids / Q000032:analysis / Q000737:chemistry; D020113:Phenolphthalein / Q000032:analysis / Q000737:chemistry; D013578:Synephrine; D053719:Tandem Mass Spectrometry
D019440:Anti-Obesity Agents; D003503:Cyclobutanes; D004365:Drugs, Chinese Herbal; D010592:Pharmaceutic Aids; D020113:Phenolphthalein; D013578:Synephrine; C058254:sibutramine