Striking species difference in the contribution of concentrative nucleoside transporter 2 to nucleoside uptake between mouse and rat hepatocytes.
Concentrative nucleoside transporter 2 (CNT2) (encoded by the SLC28A2 gene) transports various antiviral or antitumor purine nucleoside analogs to be involved in their pharmacokinetics and pharmacological actions. The results of our study showed that mouse hepatocytes hardly expressed CNT2 mRNA and no CNT2-dependent nucleoside uptake was observed, while rat hepatocytes exhibited high CNT2-dependent nucleoside uptake activity levels with abundant CNT2 mRNA expression. We concluded that CNT2 contributes considerably to nucleoside uptake in rat hepatocytes but not in mouse hepatocytes.
Furihata
Tomomi
T
Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuou-ku, Chiba-shi, Chiba, Japan 260-8675. tomomif@p.chiba-u.ac.jp
Fukuchi
Yukina
Y
Iikura
Minami
M
Hashizume
Misato
M
Miyajima
Atsushi
A
Nagai
Miki
M
Chiba
Kan
K
D000241:Adenosine / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D022781:Hepatocytes / Q000378:metabolism*; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009705:Nucleosides / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012254:Ribavirin / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
D026901:Membrane Transport Proteins; D009705:Nucleosides; C065171:cif nucleoside transporter; D012254:Ribavirin; D012964:Sodium; D000241:Adenosine