Assessment of CD8(+) T cell differentiation in Trypanosoma cruzi-infected children.
We previously reported that the T cell compartment in chronically Trypanosoma cruzi-infected adult subjects display functional and phenotypic signs of immune senescence. This study aimed to investigate the differentiation and the senescent profile of the overall CD8(+) T cell compartment in T. cruzi-infected children at the early stage of the disease. We found a lower percentage of naive (CD27(+)CD28(+)CD45RA(+)) and early antigen-experienced (CD45RA(-)CD27(+)CD28(+)), and higher percentages of late differentiated antigen-experienced (CD45RA(-)CD27(-)CD28(-)) CD8(+) T cells in T. cruzi-infected children as compared with age-matched uninfected controls. The expression of the interleukin (IL)-7R is also decreased on naive and on antigen-experienced total CD8(+) T cells with various degrees of differentiation. Conversely, the expression of HLA-DR, caspase-3, and CD57 did not vary on the total CD8(+) T cell compartment. These findings suggest that the duration of the infection is relevant in the process of immune senescent that this parasite can induce.
Albareda
María Cecilia
MC
Instituto Nacional de Parasitología "Dr. M. Fatala Chaben," Ciudad Autónoma de Buenos Aires, Argentina. mcalbareda@gmail.com
Olivera
Gabriela Carina
GC
De Rissio
Ana María
AM
Postan
Miriam
M
D000293:Adolescent; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology*; D016022:Case-Control Studies; D002454:Cell Differentiation; D014355:Chagas Disease / Q000276:immunology*; D002648:Child; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D014349:Trypanosoma cruzi / Q000276:immunology
D015703:Antigens, CD; D016207:Cytokines