title: Sequence analysis of RNA 2 and RNA 3 of lilac leaf chlorosis virus: a putative new member of the genus Ilarvirus.
abstract: RNA 2 and RNA 3 of lilac leaf chlorosis virus (LLCV) were sequenced and shown to be 2,762 nucleotides (nt) and 2,117 nts in length, respectively. RNA 2 encodes a putative 807-amino-acid (aa) RNA-dependent RNA polymerase associated protein with an estimated M (r) of 92.75 kDa. RNA 3 is bicistronic, with ORF1 encoding a putative movement protein (277 aa, M (r) 31.45 kDa) and ORF2 encoding the putative coat protein (221 aa, M (r) 24.37 kDa). The genome organization is similar to that typical for members of the genus Ilarvirus. Phylogenetic analyses indicate a close evolutionary relationship between LLCV, ApMV, and PNRSV.
authors.affiliation: Sidney Laboratory, Centre for Plant Health, Canadian Food Inspection Agency, 8801 East Saanich Road, Sidney, BC, Canada. Delano.James@inspection.gc.ca
authors.forename: D
authors.initials: D
authors.lastname: James
authors.forename: A
authors.initials: A
authors.lastname: Varga
authors.forename: L
authors.initials: L
authors.lastname: Leippi
authors.forename: S
authors.initials: S
authors.lastname: Godkin
authors.forename: C
authors.initials: C
authors.lastname: Masters
mesh_terms: D019179:Ilarvirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology*; D053830:Plant Viral Movement Proteins / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D031662:Syringa / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase