[Angiogenesis promoting effect of Morinda officinalis oligosaccharides on chicken embryo chorioallantoic membrane].
OBJECTIVE
To study the angiogenesis promoting effect of Morinda officinalis oligosaccharides(MOO) on chick embryo chorioallantoic membrane (CAM).


METHODS
Rats blood serum containing low, medium and high doses of MOO was prepared using Chinese herbs serum pharmacology method. 60 chick embryoes were randomly divided into low, medium and high doses of MOO groups, as well as NS group, blank serum group and bFGF group. Each group included 10 embryoes. CAM model was prepared after 7 days incubation. Then NS, blank serum, bFGF (2500 U x mL(-1)), three doses of serum containing MOO were added respectively onto the carriers on the CAM. CAM sample was prepared after 3 days incubation. The state of angiogenesis was observed and the number of new blood vessels was counted.


RESULTS
Compared with blank serum and NS group, a more specific CAM angiogenesis appearance could be observed in each MOO group and bFGF group. Compared with blank serum group, the number of new blood vessels in each MOO group increased significantly (P < 0.05). But the drug had a lower efficacy than bFGF (P < 0.05). Compared with low dose group, the number of new blood vessels increased significantly in medium and high doses groups (P < 0.05). But there was no significant difference between the latter two groups. The number of new blood vessels showed no significant difference between NS group and blank serum group.


CONCLUSIONS
MOO can obviously promote angiogenesis of CAM.
Yang
Jingke
J
Xiangya Second Hospital, Central South University, Changsha 410011, China.
Feng
Guoqing
G
Yu
Shuang
S
Qiao
Peng
P
D000818:Animals; D002642:Chick Embryo / Q000098:blood supply* / Q000187:drug effects*; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000098:blood supply* / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005260:Female; D008297:Male; D032066:Morinda / Q000737:chemistry*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009844:Oligosaccharides / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D009844:Oligosaccharides