PU.1-mediated upregulation of CSF1R is crucial for leukemia stem cell potential induced by MOZ-TIF2.
Leukemias and other cancers possess self-renewing stem cells that help to maintain the cancer. Cancer stem cell eradication is thought to be crucial for successful anticancer therapy. Using an acute myeloid leukemia (AML) model induced by the leukemia-associated monocytic leukemia zinc finger (MOZ)-TIF2 fusion protein, we show here that AML can be cured by the ablation of leukemia stem cells. The MOZ fusion proteins MOZ-TIF2 and MOZ-CBP interacted with the transcription factor PU.1 to stimulate the expression of macrophage colony-stimulating factor receptor (CSF1R, also known as M-CSFR, c-FMS or CD115). Studies using PU.1-deficient mice showed that PU.1 is essential for the ability of MOZ-TIF2 to establish and maintain AML stem cells. Cells expressing high amounts of CSF1R (CSF1R(high) cells), but not those expressing low amounts of CSF1R (CSF1R(low) cells), showed potent leukemia-initiating activity. Using transgenic mice expressing a drug-inducible suicide gene controlled by the CSF1R promoter, we cured AML by ablation of CSF1R(high) cells. Moreover, induction of AML was suppressed in CSF1R-deficient mice and CSF1R inhibitors slowed the progression of MOZ-TIF2-induced leukemia. Thus, in this subtype of AML, leukemia stem cells are contained within the CSF1R(high) cell population, and we suggest that targeting of PU.1-mediated upregulation of CSF1R expression might be a useful therapeutic approach.
Aikawa
Yukiko
Y
Molecular Oncology Division, National Cancer Center Research Institute, Tsukiji, Tokyo, Japan.
Katsumoto
Takuo
T
Zhang
Pu
P
Shima
Haruko
H
Shino
Mika
M
Terui
Kiminori
K
Ito
Etsuro
E
Ohno
Hiroaki
H
Stanley
E Richard
ER
Singh
Harinder
H
Tenen
Daniel G
DG
Kitabayashi
Issay
I
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D003115:Colony-Stimulating Factors / Q000235:genetics; D005796:Genes; D006801:Humans; D007938:Leukemia / Q000235:genetics; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D051958:Nuclear Receptor Coactivator 2 / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics; D016184:Receptors, Colony-Stimulating Factor / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*; D015854:Up-Regulation; D016335:Zinc Fingers / Q000235:genetics; D000099232:Proto-Oncogene Protein Spi-1
D003115:Colony-Stimulating Factors; D016173:Macrophage Colony-Stimulating Factor; D051958:Nuclear Receptor Coactivator 2; D011518:Proto-Oncogene Proteins; D016186:Receptor, Macrophage Colony-Stimulating Factor; D016184:Receptors, Colony-Stimulating Factor; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C498001:NCOA2 protein, human