In vitro assessment of antibacterial activity and cytocompatibility of silver-containing PHBV nanofibrous scaffolds for tissue engineering.
Infections with bacteria have become a serious problem in joint arthroplasty. This study reports about in vitro antibacterial activity and in vitro cell compatibility of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers loaded with metallic silver particles of a size of 5-13 nm. In vitro antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae was studied by microplate proliferation tests. The adhesion, viability, and proliferation properties of fibroblasts (NIH 3T3) and differentiation of osteoblasts (MC3T3-E1) were done to study in vitro cell compatibility of the scaffolds. As the results, only silver-containing PHBV nanofibrous scaffolds showed a high antibacterial activity and an inhibitory effect on the growth of both Staphylococcus aureus and Klebsiella pneumoniae bacteria. The nanofibrous scaffolds having silver nanoparticles <1.0% were free of in vitro cytotoxicity. To sum up, the PHBV nanofibrous scaffolds having nanoparticles <1.0 wt % showed not only good antibacterial activity but also good in vitro cell compatibility. It is considered that the PHBV nanofibrous scaffolds with silver nanoparticles <1.0 wt % have a potential to be used in joint arthroplasty.
Xing
Zhi-Cai
ZC
Department of Polymer Science and Engineering, Kyungpook National University, Daegu 702-701, Korea.
Chae
Won-Pyo
WP
Baek
Jin-Young
JY
Choi
Moon-Jeong
MJ
Jung
Yongsoo
Y
Kang
Inn-Kyu
IK
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001672:Biocompatible Materials; D007711:Klebsiella pneumoniae / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D049329:Nanostructures; D012834:Silver / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D023822:Tissue Engineering
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D012834:Silver