Growth and maintenance of baby hamster kidney (BHK) cells.
The BHK21 cell line was established in 1961 from the kidneys of 5 Syrian hamsters from litter number 21. Since this time, this cell line has been a laboratory standard for the growth of countless viruses and the study of many biological processes. The specific use for the growth of Sindbis virus is described, although it may apply to many types of viruses.
Hernandez
Raquel
R
North Carolina State University, Raleigh, North Carolina, USA.
Brown
Dennis T
DT
D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D006224:Cricetinae; D003470:Culture Media / Q000737:chemistry; D005347:Fibroblasts; D007668:Kidney / Q000166:cytology; D008647:Mesocricetus; D012845:Sindbis Virus / Q000254:growth & development; D014776:Virus Cultivation / Q000379:methods*
D003470:Culture Media