Pulmonary blast injury in mice: a novel model for studying blast injury in the laboratory using laser-induced stress waves.
OBJECTIVE
Primary blast injury is produced by shock waves. Blast injuries to lungs are extremely critical threats to survival, but their etiology is largely undefined. The majority of animal models for these injuries use explosive or complex experimental settings, limiting the laboratory study of blast injury. The aim of this study was to establish a small-animal model for blast injuries, using laser-induced stress waves (LISWs) with high controllability, high reproducibility, and easy experimental settings.


METHODS
LISWs were used to produce isolated pulmonary blast effects in mice. An LISW was generated by the irradiation of an elastic laser target with 532-nm nanosecond laser pulses of a Q-switched Nd:YAG laser. Histopathological evaluations of damage to lung tissue were conducted to estimate the relevance between peak pressure and trauma intensity. Blood pressure, heart rate, and percutaneous oxygen saturation were monitored for 60 minutes.


RESULTS
We could flexibly control the peak pressure of the shock wave by varying the laser energy. Non-lethal doses of LISWs caused pulmonary contusions with alveolar hemorrhages depending on peak pressure. Pulmonary contusion was observed only in areas that were exposed to LISWs, allowing study of isolated injuries without concomitant ones. These injuries caused decreased blood pressure, heart rate, and percutaneous oxygen saturation, immediately after LISW exposure.


CONCLUSIONS
Mice exposed to thoracic LISWs showed pathologic and physiologic changes similar to those seen in other studies in this area, and in clinical practice. Our newly developed model allows fine management of trauma intensity, and concomitant injuries of the exposed animals were limited. This novel mouse model of blast injury using LISWs is suitable for detailed studies of blast lung contusion and other blast injuries in the laboratory.
Satoh
Yasushi
Y
Department of Biochemistry, National Defense Medical College, 3-2 Namiki, Tokorozawa 359-8513, Japan. ys@ndmc.ac.jp
Sato
Shunichi
S
Saitoh
Daizoh
D
Tokuno
Shinichi
S
Hatano
Ben
B
Shimokawaji
Tadasuke
T
Kobayashi
Hideo
H
Takishima
Kunio
K
D000704:Analysis of Variance; D000818:Animals; D001753:Blast Injuries / Q000503:physiopathology*; D004195:Disease Models, Animal; D007834:Lasers; D055370:Lung Injury / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL