title: Histological changes of rat soft palate with exposure to experimental laryngopharyngeal reflux.
abstract: OBJECTIVE
  The possible effects of laryngopharyngeal reflux (LPR) on laryngeal and otologic disorders have been studied in the literature. There have been no reports explaining the possible effects of LPR on the soft palate. Therefore, in this study, we investigated the histopathologic changes in the rat soft palate using an experimental model of reflux.
  
  
  METHODS
  Eighteen healthy 200-220-g 20-week-old Sprague-Dawley rats were used. The animals were divided into three groups according to exposure time (1, 4, and 12 week exposures), and four rats were examined as controls who had undergone sham operation. An experimental model of gastroesophageal reflux was induced under general anesthesia. After exposure, the animals were sacrificed, and their soft palates were removed. The histopathological changes in the soft palates were observed under a light microscope.
  
  
  RESULTS
  Submucous gland hyperplasia, inflammation, subepithelial edema, vascular engorgement, muscular atrophy and dilated glandular excretory duct were compared among the groups. Submucous gland hyperplasia, subepithelial edema, inflammation, vascular engorgement, muscular atrophy and dilated glandular excretory duct were significantly different in the exposure groups compared with the control group.
  
  
  CONCLUSIONS
  On the basis of histopathological evaluations, our findings suggest that reflux affects the soft palate, which suggests that these pathological changes may reflect the relationship between LPR and airway obstruction.
authors.affiliation: Haydarpasa Numune Education and Research Hospital, Department of Otorhinolaryngology, Istanbul, Turkey. tulayhabesoglu@yahoo.com
authors.forename: Tulay Erden
authors.initials: TE
authors.lastname: Habesoglu
authors.forename: Mehmet
authors.initials: M
authors.lastname: Habesoglu
authors.forename: Mehmet
authors.initials: M
authors.lastname: Sürmeli
authors.forename: Ildem
authors.initials: I
authors.lastname: Deveci
authors.forename: Sema Zer
authors.initials: SZ
authors.lastname: Toros
authors.forename: Pempegul
authors.initials: P
authors.lastname: Gunes
authors.forename: Halil
authors.initials: H
authors.lastname: Tugtepe
authors.forename: Erol
authors.initials: E
authors.lastname: Egeli
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000473:pathology; D004487:Edema / Q000209:etiology / Q000473:pathology; D006965:Hyperplasia; D057045:Laryngopharyngeal Reflux / Q000150:complications / Q000473:pathology*; D009061:Mouth Mucosa / Q000473:pathology; D009133:Muscular Atrophy / Q000209:etiology / Q000473:pathology; D010156:Palatal Muscles / Q000473:pathology; D010160:Palate, Soft / Q000098:blood supply / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013280:Stomatitis / Q000209:etiology / Q000473:pathology; D013997:Time Factors