Biomineralization-assisted ultrasensitive detection of DNA.
The development of instrument-free, PCR-less, ultrasensitive and selective DNA detection methods is highly desired in chemical and life sciences. Herein we report on the utility of a biomineralization-assisted amplification methodology for the identification of DNA. Significantly, the diagnostic strategy has allowed the target detection at a concentration as low as 50 aM, equivalent to approximately 180 copies in the entire 6 microL sample. In addition, the DNA sequence with a single-base mismatch can be differentiated from the perfect target through a facile salt-based stringency wash. Substitution of the DNA structures with other recognition moieties should allow the translation of the strategy to the assay of different targets of interest. The visual readout format provides a sound basis for the broad applicability of the proposed strategy, especially in resource-poor settings.
Zhou
Xin
X
Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China.
Xia
Sijing
S
Lu
Zhiqiang
Z
Tian
Yuan
Y
Yan
Yishu
Y
Zhu
Jin
J
D020137:Base Pair Mismatch; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D008903:Minerals / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization
D008903:Minerals; D006046:Gold; D004247:DNA