KITENIN represents a more aggressive phenotype in a murine model of oral cavity squamous carcinoma.
OBJECTIVE
To investigate the tumor invasiveness and early lung metastasis associated with KITENIN in a murine model of oral cavity squamous carcinoma.


METHODS
Cross-sectional study with planned data collection.


METHODS
Department of Otolaryngology-Head and Neck Surgery, Medical Research Center for Gene Regulation, and Pathology, Chonnam National University Medical School and Hwasun Hospital.


METHODS
The cDNA of KITENIN and vector only were transfected into the SCC VII (murine squamous cell line) cells. The suspension of 5 x 10(5)/mL viable KITENIN- or vector-transfected SCC VII cells was injected into the floor of the mouth of C3H/HeJ syngeneic mice, deep into the mylohyoid muscle via the intraoral approach. From the first to the sixth week after injection, tumor, lung, liver, and brain tissues were obtained.


RESULTS
For all groups, the tumor invaded the superficial musculature of the floor of the mouth, the deep musculature of the floor of the mouth, the salivary glands, perineural tissue, bone, and skin, sequentially. Lung metastases developed multiple focal nodular patterns at first and then became more extensive. Earlier and more aggressive tumor invasion into the deep floor of the mouth musculature, salivary glands, perineural tissue, bone, and skin was observed in the KITENIN-transfected group compared to the vector-only group. An earlier and more extensive lung metastasis was observed in the KITENIN group.


CONCLUSIONS
An antisense KITENIN strategy might inhibit distant metastasis and the progression of head and neck squamous cell carcinoma.
Lee
Joon Kyoo
JK
Department of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School and Hwasun Hospital, Hwasungun, Jeonnam, Korea. joonkyoo@chonnam.ac.kr
Lim
Sang Chul
SC
Kim
Hee Dae
HD
Yoon
Tae Mi
TM
Kim
Kag
K
Nam
Jong Hee
JH
Kim
Hyung Seok
HS
Shin
Min-Ho
MH
Kim
Kyung Keun
KK
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006258:Head and Neck Neoplasms / Q000473:pathology; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000556:secondary*; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D009062:Mouth Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness; D014162:Transfection
D008565:Membrane Proteins