Effect of hepatic blood inflow occlusion without hemihepatic artery control on apoptosis of liver cells in rats.
OBJECTIVE
To investigate the effect of hepatic blood inflow occlusion without hemihepatic artery control on the apoptosis of liver cells during ischemia-reperfusion injury in rats.


METHODS
Animal models of three hepatic vascular occlusion methods were established. Hepatic blood inflow occlusion without hemihepatic artery control (WH group), hemihepatic vascular occlusion (HH group) and Pringle maneuver (PR group) were compared.


RESULTS
At each time point after reperfusion, ALT, AST, and apoptosis rates were significantly higher in the PR group than in the HH group or the WH group (p < 0.01), and the pathology and hepatocyte ultrastructure at 6 hours after reperfusion were significantly worse in the PR group than in the HH group or the WH group (p < 0.01). ALT, AST, apoptosis rate, pathology and hepatocyte ultrastructure at 6 hours after reperfusion were not significantly different between the WH group and the HH group (p > 0.05).


CONCLUSIONS
Hepatic blood inflow occlusion without hemihepatic artery control can reduce hepatic ischemia-reperfusion injury, and the effect is similar to that of hemihepatic vascular occlusion.
Jin
Shan
S
Department of General Surgery, Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010050, China. altten_aull@yahoo.com.cn
Dai
Chaoliu
C
Yang
Yuntian
Y
Zhang
Ruiming
R
Sileng
A
A
D000818:Animals; D017209:Apoptosis; D003250:Constriction; D004195:Disease Models, Animal; D004210:Dissection; D006488:Hemostasis, Surgical; D006498:Hepatectomy / Q000009:adverse effects / Q000379:methods*; D006499:Hepatic Artery; D022781:Hepatocytes / Q000473:pathology* / Q000648:ultrastructure; D011169:Portal Vein; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology* / Q000517:prevention & control*