title: Liquid chromatographic separation of pregabalin and its possible impurities with fluorescence detection after postcolumn derivatization with o-phtaldialdehyde.
abstract: A rapid procedure based on direct extraction and RP-HPLC separation of pregabalin and its possible impurities with fluorescence detection has been developed. The separation conditions and parameters of derivatization reaction for postcolumn derivatization of pregabalin with o-phtaldialdehyde/2-mercaptoethanol were studied. Purospher STAR RP-8e column with isocratic elution was employed. Fluorescence detection was performed at excitation and emission wavelength of 345 nm and 450 nm, respectively. The proposed method has an advantage of a simple sample pre-treatment and a quick and very sensitive HPLC method. The applicability of developed method was successfully verified during analysis of commercial samples of tablets of Lyrica (Pfizer, USA).
authors.affiliation: Zentiva, as Praha, U Kabelovny 130, 102 37 Praha 10, Czech Republic. hplc@hplc.cz
authors.forename: Michal
authors.initials: M
authors.lastname: Dousa
authors.forename: Petr
authors.initials: P
authors.lastname: Gibala
authors.forename: K
authors.initials: K
authors.lastname: Lemr
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004340:Drug Contamination; D057230:Limit of Detection; D000069583:Pregabalin; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D009764:o-Phthalaldehyde
chemical_list: D000069583:Pregabalin; D005680:gamma-Aminobutyric Acid; D009764:o-Phthalaldehyde