title: L-type calcium channel CaV 1.2 in transgenic mice overexpressing human AbetaPP751 with the London (V717I) and Swedish (K670M/N671L) mutations.
abstract: Cumulative evidence indicates that amyloid-beta peptides exert some of their neurodegenerative effects through modulation of L-type voltage gated calcium channels, which play key roles in a diverse range of CNS functions. In this study we examined the expression of CaV1.2 L-type voltage gated calcium channels in transgenic mice overexpressing human AbetaPP751 with the London (V717I) and Swedish (K670M/N671L) mutations by immunohistochemistry in light and electron microscopy. In hippocampal layers of wild type and transgenic mice, CaV1.2 channels were predominantly localized to somato-dendritic domains of neurons, and to astrocytic profiles with an age-dependent increase in labeling density. In transgenic animals, CaV1.2-like immunoreactive clusters were found in neuronal profiles in association with amyloid-beta plaques. Both the number and density of these clusters depended upon age of animals and number of plaques. The most striking difference between wild type and transgenic mice was the age-dependent expression of CaV1.2 channels in reactive astrocytes. At the age of 6 month, CaV1.2 channels were rarely detected in reactive astrocytes of transgenic mice, but an incremental number of CaV1.2 expressing reactive astrocytes was found with increasing age of animals and number of amyloid-beta plaques. This study demonstrates that CaV1.2 channels are highly expressed in reactive astrocytes of 12-months of age transgenic mice, which might be a consequence of the increasing amyloid burden. Further studies should clarify which functional implications are associated with the higher availability of CaV1.2 channels in late stage Alzheimer's disease.
authors.affiliation: Department of Psychiatry and Psychotherapy, University Clinic of General and Social Psychiatry, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Willis
authors.forename: Walter A
authors.initials: WA
authors.lastname: Kaufmann
authors.forename: Georg
authors.initials: G
authors.lastname: Wietzorrek
authors.forename: Birgit
authors.initials: B
authors.lastname: Hutter-Paier
authors.forename: Sven
authors.initials: S
authors.lastname: Moosmang
authors.forename: Christian
authors.initials: C
authors.lastname: Humpel
authors.forename: Franz
authors.initials: F
authors.lastname: Hofmann
authors.forename: Manfred
authors.initials: M
authors.lastname: Windisch
authors.forename: Hans-Günther
authors.initials: HG
authors.lastname: Knaus
authors.forename: Josef
authors.initials: J
authors.lastname: Marksteiner
mesh_terms: D000375:Aging / Q000473:pathology; D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis* / Q000235:genetics*; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D001921:Brain / Q000473:pathology / Q000648:ultrastructure; D020746:Calcium Channels, L-Type / Q000235:genetics* / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008854:Microscopy, Electron; D009154:Mutation; D016501:Neurites / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D016564:Amyloid beta-Protein Precursor; C514350:CACNA1C protein, mouse; D020746:Calcium Channels, L-Type