Fluorescence detection for DNA using hybridization chain reaction with enzyme-amplification.
A signal amplification based on a combination of hybridization chain reaction (HCR) and enzyme-enhancement was applied in the study of a novel biosensor. The target sequence was added as catalyst which was simply transmitted but did not disappear as the DNA double strand grew on the surface of magnetic beads (MBs).
Niu
Shuyan
S
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, PR China.
Jiang
Yu
Y
Zhang
Shusheng
S
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D057230:Limit of Detection; D009693:Nucleic Acid Hybridization / Q000379:methods*; D013050:Spectrometry, Fluorescence
D004247:DNA