title: Two-dimensional surface properties of an antimicrobial hydantoin at the air-water interface: an experimental and theoretical study.
abstract: 5,5-Acetamidomethyl-5-methylimidazolidine-2,4-dione shows antimicrobial activity against bacteria at millimolar concentrations well above its critical micellar concentration (cmc). Two-dimensional surface properties were investigated using Langmuir Film Balance to understand the membrane-active nature and nanomaterial behavior of this hydantoin derivative. Hydantoin forms an expanded nanofilm at air-water interface. The maximum limiting surface area (A(0)) and collapse pressure (pi(c)) are dependent on hydantoin concentration. Hydantoin undergoes a change in orientation at the interface, in the pressure region 2.5 and 7.5 mNm(-1), corresponding to surface areas 51-15 and 41-12A(2)molecule(-1), respectively. A large collapse pressure (pi(c)) in LB film indicates a role for hydrophobic interactions in the self-assembly of hydantoin. Surface areas computed using Connolly method, are in good agreement with the experimental results. Monolayer studies suggest a dispersed state for hydantoin when its concentration is below cmc, suggesting a mechanism for the observed bacteriostatic activity of hydantoin. In the present study, it has been found for the first time that the minimum inhibitory concentration (MIC) of the hydantoin is very close to its cmc.
authors.affiliation: Chemical Laboratory, Physical and Inorganic Chemistry Division, Central Leather Research Institute, Council of Scientific and Industrial Research (CSIR), Adyar, Chennai 600020, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Mandal
authors.forename: Ramya Santhana Gopala
authors.initials: RS
authors.lastname: Krishnan
authors.forename: Sathiah
authors.initials: S
authors.lastname: Thennarasu
authors.forename: Satya
authors.initials: S
authors.lastname: Panigrahi
authors.forename: Asit Baran
authors.initials: AB
authors.lastname: Mandal
mesh_terms: D000388:Air; D000465:Algorithms; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D006827:Hydantoins / Q000737:chemistry* / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D008567:Membranes, Artificial; D008826:Microbial Sensitivity Tests; D008956:Models, Chemical; D015394:Molecular Structure; D011312:Pressure; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D006827:Hydantoins; D008567:Membranes, Artificial; D014867:Water