Riboflavin protects mice against liposaccharide-induced shock through expression of heat shock protein 25.
Riboflavin (vitamin B2) is a water-soluble vitamin essential for normal cellular functions, growth and development. This study aimed to investigate the effects of vitamin B2 on the survival rate, and expressions of tissue heat shock protein 25 (HSP25) and heat shock factor 1 (HSF1) in mice undergoing lipopolysaccharide (LPS) induced shock. Mice were assigned to four groups, saline vehicle, LPS, LPS plus low dose of vitamin B2 (LB2) and LPS plus high dose of vitamin B2 (HB2). Vitamin B2 (1 and 10mg/kg BW) was administered intraperitoneally at 2 and 0 h before the i.p. administration of LPS. At the end of the experiment, the survival rate monitored was 10, 20, 60, and 100% for LPS, LB2, HB2, and saline mice, respectively. HSP25 expressions in the heart and lung were significantly enhanced in a time-dependent manner in the HB2 mice as compared to the saline mice (p < 0.05), but not altered in the LB2 mice. In the HB2 mice, plasma riboflavin concentrations reached 300 nM at 6h post LPS and returned to the 0 h level at 72 h. The results showed that high dose of riboflavin could decrease LPS-induced mortality through an increased expression of HSP25.
Shih
Chun-Kuang
CK
School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, Taiwan, ROC.
Chen
Chiao-Ming
CM
Chen
C-Y Oliver
CY
Liu
Jen-Fang
JF
Lin
Hui-Wen
HW
Chou
Hung-Tsung
HT
Li
Sing-Chung
SC
D000818:Animals; D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000502:physiology*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018832:Molecular Chaperones; D009206:Myocardium / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000502:physiology*; D009687:Nuclear Proteins / Q000096:biosynthesis; D012256:Riboflavin / Q000097:blood / Q000494:pharmacology*; D012772:Shock, Septic / Q000139:chemically induced / Q000517:prevention & control*; D015996:Survival Rate; D014157:Transcription Factors / Q000378:metabolism; D014815:Vitamins / Q000097:blood / Q000494:pharmacology*
D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; C486113:Hsbp1 protein, mouse; C490017:Hsf1 protein, mouse; D008070:Lipopolysaccharides; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; D014815:Vitamins; D012256:Riboflavin