In vivo administration of acepromazine or promethazine to horse decreases the reactive oxygen species production response of subsequently isolated neutrophils to stimulation with phorbol myristate acetate.
The previous experiments have shown that some phenothiazines have antioxidant and anti-inflammatory properties in vitro. In this study the inhibition of the production of reactive oxygen species (ROS) by neutrophils was studied in two groups of horses, which received a dose of 0.1 mg/kg of either acepromazine or promethazine intravenously. Blood samples were collected before (T0) and 0.5, 1, 3 and 5 h after drug administration. The chemiluminescence (CML) response of neutrophils was measured ex vivo in the presence of luminol for a period of 10 min and the maximum CML value (peak value) recorded. There was a significant inhibition of the ROS production in the acepromazine treated group (49% inhibition) at 5 h after administration and in the promethazine group (24% inhibition) at 3 h after administration (P < 0.05 vs. T0). These findings are of therapeutic relevance in the use of phenothiazines in equine patients with inflammatory diseases where neutrophil activation and ROS production are implicated.
Péters
F
F
University of Liège, Faculty of Veterinary Medicine, Equine Clinic, B41, 4000 Liège 1, Belgium.
Franck
T
T
Pequito
M
M
de la Rebière
G
G
Grulke
S
S
Salccicia
A
A
Verwilghen
D
D
Chiavaccini
L
L
Deby-Dupont
G
G
Serteyn
D
D
D000075:Acepromazine / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D018492:Dopamine Antagonists / Q000494:pharmacology; D006736:Horses; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011398:Promethazine / Q000008:administration & dosage / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000975:Antioxidants; D018492:Dopamine Antagonists; D017382:Reactive Oxygen Species; D000075:Acepromazine; D011398:Promethazine; D013755:Tetradecanoylphorbol Acetate