The effect of electrospun fibre alignment on the behaviour of rat periodontal ligament cells.
It is envisioned that for the regeneration of highly organized structures, like tendon and ligaments, only aligned fibrous scaffolds can provide adequate topographic guidance to cells. In this study, a novel method to electrospin an aligned scaffold is presented. Electrospun fibres were deposited into a water bath and then the fibres were drawn to a rotating mandrel in a controlled manner. In this way, parallel and cross-aligned fibrous poly (lactide-co-glycolide) (PLGA) scaffolds were fabricated, which were subsequently used to study their effect on the growth behaviour of rat periodontal ligament (PDL) cells. First, the scaffolds were characterized regarding mechanical properties, scaffold stability and degradation in vitro. Then, rat PDL cells were seeded and cultured on these scaffolds for up to 7 days. Randomly oriented PLGA and solvent cast plain PLGA films served as controls. Results showed that the alignment of fibres resulted in a higher tensile stress and Young's modulus. Aligned scaffolds maintained their structural stability better compared to the controls after incubation in phosphate-buffered saline for 6 weeks. Further, cells were observed to elongate along the fibre after 3 days of culture. Proliferation and migration of PDL cells was significantly more prevalent on the aligned fibres compared to the controls. It was concluded that aligned scaffolds seem to be able to promote the organized regeneration of periodontal tissue.
Shang
Shuhuan
S
Department of Biomaterials, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
Yang
Fang
F
Cheng
Xiangrong
X
Walboomers
X Frank
XF
Jansen
John A
JA
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques; D019344:Lactic Acid / Q000737:chemistry; D008968:Molecular Conformation; D010513:Periodontal Ligament / Q000166:cytology* / Q000502:physiology; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D012038:Regeneration; D044968:Regenerative Medicine / Q000379:methods; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry* / Q000592:standards
D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid