Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake.
In this study, structural features of Tricholoma matsutake polysaccharide (TMP-A) were investigated by a combination of infrared (IR) spectra, gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR) spectroscopy. The results indicated that TMP-A had a backbone of 1,4-beta-d-glucopyranose residue which branches at O-6 based on the experimental results. The branches were mainly composed of an (1-->3)-alpha-d-galactopyranose residue, and terminated with alpha-d-xylopyranose residue. The antioxidant activity of TMP-A was evaluated with several biochemical methods, including DPPH(-) radical scavenging, hydrogen peroxide scavenging, superoxide anion radical scavenging. The results indicated that TMP-A showed strong antioxidant. In the in vitro antioxidant assay by MTT method, TMP-A could attenuate PC12 cell damage significantly caused by hydrogen peroxide.
Ding
Xiang
X
Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, PR China.
Tang
Jie
J
Cao
Mei
M
Guo
Chun-Xiao
CX
Zhang
Xia
X
Zhong
Jing
J
Zhang
Jie
J
Sun
Qun
Q
Feng
Su
S
Yang
Zhi-Rong
ZR
Zhao
Jian
J
D001713:Biphenyl Compounds / Q000378:metabolism; D002240:Carbohydrate Sequence; D002845:Chromatography; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D017665:Hydroxyl Radical / Q000378:metabolism; D008969:Molecular Sequence Data; D010851:Picrates / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D012995:Solubility; D013481:Superoxides / Q000378:metabolism; D055439:Tricholoma / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D014867:Water; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl