5.4 nm spatial resolution in biological photoemission electron microscopy.
We report a spatial resolution of 5.4 nm in images of sarcoplasmic reticulum from rabbit muscle. The images were obtained in an aberration-corrected photoemission electron microscope with a hyperbolic mirror as the correcting element for spherical and chromatic aberration. In-situ measurements and numerical simulations confirm the low residual aberration in the instrument and indicate the ultimate resolution in this type of microscopy to be below 2 nm.
Könenkamp
R
R
Physics Department, Portland State University, 1719 SW 10th Avenue, Portland, OR 97201, USA. rkoe@pdx.edu
Word
Robert C
RC
Rempfer
G F
GF
Dixon
T
T
Almaraz
L
L
Jones
T
T
D000818:Animals; D004867:Equipment Design; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure