Development of a high-throughput method for the determination of ethosuximide in human plasma by liquid chromatography mass spectrometry.
A simple, rapid, sensitive and specific ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantification of ethosuximide in human plasma is described. Analyte was chromatographed on a Hypersil Gold C18 column (100 mm x 2.1 mm, i.d., 1.9 microm) with isocratic elution at a flow rate of 0.250 mL/min and pravastatin was used as the internal standard. The assay involves a simple solid-phase extraction procedure of 0.25 mL human plasma and the analysis was performed on a triple-quadrupole tandem mass spectrometer by MRM mode via electrospray ionization (ESI). The method was linear in the concentration range of 0.25-60.0 microg/mL. The lower limit of quantification (LLOQ) was 0.25 microg/mL. The within- and between-day precision and accuracy of the quality control samples were within 10.0%. The recovery was 95.1% and 94.4% for ethosuximide and pravastatin, respectively. The analysis time for each sample was 1.8 min. The method was highly reproducible and gave peaks with excellent chromatography properties.
Bhatt
Mitesh
M
Department of Chemistry, Shree U.P. Arts, Smt. M.G. Panchal Science & V.L. Shah Commerce College Pilvai, Vijapur, Gujarat State, India. mitu 070980@yahoo.co.in
Shah
Sanjay
S
Shivprakash
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005013:Ethosuximide / Q000097:blood* / Q000737:chemistry; D006801:Humans; D016018:Least-Squares Analysis; D017035:Pravastatin / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
D005013:Ethosuximide; D017035:Pravastatin