Oxygen-independent photonuclease activity of a new iron(II) complex.
A new iron(ii) complex was synthesized and its photonuclease activity against plasmid DNA was evaluated. After kinetic experiments a k(cat) of 18.86 +/- 2.9 h(-1) was obtained, one of the highest nuclease activities of synthetic metallonucleases, selectively activated by 365 nm UV light.
de Souza
Bernardo
B
Departamento de Química, Universidade Federal de Santa Catarina, 88040-900, Florianópolis, SC, Brazil.
Xavier
Fernando R
FR
Peralta
Rosely A
RA
Bortoluzzi
Adailton J
AJ
Conte
Gilmar
G
Gallardo
Hugo
H
Fischer
Franciele L
FL
Bussi
Giselle
G
Terenzi
Hernán
H
Neves
Ademir
A
D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D053837:DNA Cleavage; D003851:Deoxyribonucleases / Q000737:chemistry / Q000378:metabolism; D005296:Ferrous Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry*; D010782:Photolysis; D010957:Plasmids / Q000737:chemistry; D014466:Ultraviolet Rays
D005296:Ferrous Compounds; D008024:Ligands; D004247:DNA; D003851:Deoxyribonucleases; D010100:Oxygen