Genetic and biological characterization of avian influenza H5N1 viruses isolated from wild birds and poultry in Western Siberia.
Three viruses included in the study were isolated from dead birds (A/duck/Omsk/1822/2006, A/chicken/Reshoty/02/2006, and A/duck/Tuva/01/2006), whereas the virus A/common gull/Chany/P/2006 was isolated from an apparently healthy gull during outbreaks of highly pathogenic avian influenza in Russia in 2006. The intravenous pathogenicity index (IVPI) of viruses A/duck/Omsk/1822/2006, A/chicken/Reshoty/02/2006, and A/duck/Tuva/01/2006 ranged from 2.7 to 3.0, while the virus A/common gull/Chany/P/2006 had a markedly lower IVPI of 1.7. The virus A/common gull/Chany/P/2006 had a unique pattern of six amino acid substitutions in the regions of viral proteins crucial for virulence of H5N1 viruses. We hypothesize that these substitutions may affect the pathogenicity of A/common gull/Chany/P/2006.
Sharshov
Kirill
K
FSRI SRC VB Vector, Novosibirsk, Russia. sharshov@yandex.ru
Romanovskaya
Alesia
A
Uzhachenko
Roman
R
Durymanov
Alexander
A
Zaykovskaya
Anna
A
Kurskaya
Olga
O
Ilinykh
Philipp
P
Silko
Nikita
N
Kulak
Mikhail
M
Alekseev
Alexander
A
Zolotykh
Sergei
S
Shestopalov
Alexander
A
Drozdov
Iliya
I
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001717:Birds; D002645:Chickens; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000453:epidemiology / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010802:Phylogeny; D012800:Siberia / Q000453:epidemiology; D013047:Specific Pathogen-Free Organisms; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014774:Virulence
D014764:Viral Proteins