[Determination of paprazine in Polygonum orientale of different commodities by HPLC].
OBJECTIVE
To establish a method for the content determination of paprazine in Polygonum orientale, and provide a new evaluation index for the quality control for P. orientale.


METHODS
HPLC was carried out on Agilent C18 (4.6 mm x 150 mm, 5 microm) with methanol-water (40:60) as mobile phase. The flow rate was 1.0 mL x min(-1) and the wavelength of UV detector was set at 290 nm.


RESULTS
The calibration curve was linear in the range of 0. 038-0.190 microg (r = 0.9999). The average recovery was 98.7% with the RSD of 2.36%.


CONCLUSIONS
The method appears to be simple, accurate and reproducible, it can be used to determine the content of paprazine in P. orientale.
Song
Jie
J
Liaoning University of Traditional Chinese Medicine, Dalian 116600. linasongjie@126.com
Zhai
Yanjun
Y
Wang
Rongxiang
R
Li
Na
N
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D028502:Polygonum / Q000737:chemistry*
D004365:Drugs, Chinese Herbal