Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography.
Following its market introduction in 1982, the cross-linked 12% agarose gel media Superose 12 has become widely known as a tool for size exclusion chromatography of proteins and other biological macromolecules. In this review it is shown that, when appropriate mobile phases are used, Superose possesses adsorption properties similar to that of traditional media for hydrophilic interaction liquid chromatography (HILIC). This is illustrated by the separation and purification of low molecular weight compounds such as polyphenols including active components of traditional Chinese medicinal herbs and green tea. Structural features of the cross-linked agarose that likely cause the observed adsorption effects are discussed as well. These are identified as being primarily ether bonds acting as strong hydrogen bond acceptors as well as hydrophobic residues originating from the cross-linking reagents.
Tan
Tianwei
T
Department of Bioengineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
Su
Zhi-Guo
ZG
Gu
Ming
M
Xu
Jun
J
Janson
Jan-Christer
JC
D000097:Acetonitriles / Q000737:chemistry; D000327:Adsorption; D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D045468:Fallopia japonica / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008855:Microscopy, Electron, Scanning; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D059808:Polyphenols; D027561:Salvia miltiorrhiza / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry*; D013662:Tea / Q000737:chemistry
D000097:Acetonitriles; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D013662:Tea; D002392:Catechin; D012685:Sepharose; C049225:Superose 12; C045651:epigallocatechin gallate; C032159:acetonitrile