Correlation between images of silent brain infarction, carotid atherosclerosis and white matter hyperintensity, and plasma levels of acrolein, IL-6 and CRP.
OBJECTIVE
We found previously that the measurement of plasma levels of protein-conjugated acrolein (PC-Acro) together with IL-6 and CRP can be used to identify silent brain infarction (SBI) with high sensitivity and specificity. The aim of this study was to clarify how three biochemical markers are correlated to SBI, carotid atherosclerosis (CA) and white matter hyperintensity (WMH).


METHODS
The levels of PC-Acro, IL-6 and CRP in plasma were measured by ELISA. SBI and WMH were evaluated by MRI, and CA was evaluated by duplex carotid ultrasonography.


RESULTS
A total of 790 apparently healthy volunteers were classified into 260 control, 214 SBI, 263 CA and 245 WMH subjects, which included 187 subjects with two or three pathologies. When the combined measurements of PC-Acro, IL-6 and CRP were evaluated together with age, using a receiver operating characteristic curve and artificial neural networks, the relative risk value (RRV), an indicator of tissue damage, was in the order SBI with CA (0.90)>SBI (0.80)>CA (0.76)>WMH with CA (0.65)>WMH (0.46)>control (0.14). RRV was also correlated with severity in each group of SBI, CA and WMH.


CONCLUSIONS
The RRV supports the idea that the degree of risk to develop a stroke is in the order SBI>CA>WMH.
Yoshida
Madoka
M
Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba 260-8675, Japan.
Higashi
Kyohei
K
Kobayashi
Eiichi
E
Saeki
Naokatsu
N
Wakui
Kenji
K
Kusaka
Tadafumi
T
Takizawa
Hirotaka
H
Kashiwado
Koichi
K
Suzuki
Norikazu
N
Fukuda
Kazumasa
K
Nakamura
Takao
T
Watanabe
Sakae
S
Tada
Kei
K
Machi
Yoshiki
Y
Mizoi
Mutsumi
M
Toida
Toshihiko
T
Kanzaki
Tetsuto
T
Tomitori
Hideyuki
H
Kashiwagi
Keiko
K
Igarashi
Kazuei
K
D000171:Acrolein / Q000097:blood*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000473:pathology*; D001927:Brain Diseases / Q000473:pathology; D020520:Brain Infarction / Q000473:pathology*; D002097:C-Reactive Protein / Q000096:biosynthesis*; D002340:Carotid Artery Diseases / Q000473:pathology*; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000097:blood*; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D008875:Middle Aged; D009413:Nerve Fibers, Myelinated / Q000473:pathology*; D016571:Neural Networks, Computer; D012307:Risk Factors
D015850:Interleukin-6; D000171:Acrolein; D002097:C-Reactive Protein