title: International multicenter study to assess a panel of reference materials for quantification of simian immunodeficiency virus RNA in plasma.
abstract: An international multicenter study was conducted to assess the performance of a panel of simian immunodeficiency virus (SIV) RNA reference materials for plasma viral load determinations. Reliable quantification was demonstrated across an approximately 6 log(10) dynamic range. Availability of external reference materials will enable independent calibration of SIV plasma viral load assays.
authors.affiliation: Division of Retrovirology, NIBSC/HPA, South Mimms, Potters Bar, Hertfordshire, United Kingdom.
authors.forename: Claire
authors.initials: C
authors.lastname: Ham
authors.forename: Priya
authors.initials: P
authors.lastname: Srinivasan
authors.forename: Rigmor
authors.initials: R
authors.lastname: Thorstensson
authors.forename: Ernst
authors.initials: E
authors.lastname: Verschoor
authors.forename: Zahra
authors.initials: Z
authors.lastname: Fagrouche
authors.forename: Leonardo
authors.initials: L
authors.lastname: Sernicola
authors.forename: Artur
authors.initials: A
authors.lastname: Ramos
authors.forename: Fausto
authors.initials: F
authors.lastname: Titti
authors.forename: Neil
authors.initials: N
authors.lastname: Almond
authors.forename: Neil
authors.initials: N
authors.lastname: Berry
mesh_terms: D000818:Animals; D016014:Linear Models; D008252:Macaca fascicularis / Q000821:virology; D012367:RNA, Viral / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012680:Sensitivity and Specificity; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D012367:RNA, Viral