title: Isolation and characterization of biogenic amine-producing bacteria in fermented soybean pastes.
abstract: Biogenic amines (BAs) are produced primarily by microorganisms found in fermented foods and are often implicated in food poisoning. BA-producing bacteria found in fermented soybean pastes were isolated and characterized using a decarboxylating medium and multiplex PCR analysis. Two BA-producing bacteria were isolated from traditional soybean pastes: one was a histamine-producing Clostridium strain, and the other was a tyramine-producing Pseudomonas strain. The Clostridium strain was determined to be a potent histamine producer among the cultures tested. Synthesis of tyramine by Pseudomonas sp. T1 was observed for the first time in this study.
authors.affiliation: Department of Food Science and Technology, Research Center for Bio Resource and Health, Chungbuk National University, Cheongju, 361-763, Republic of Korea.
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Moon
authors.forename: Seung Kee
authors.initials: SK
authors.lastname: Cho
authors.forename: Hwa Young
authors.initials: HY
authors.lastname: Choi
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: So-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Kyung-Ju
authors.initials: KJ
authors.lastname: Cho
authors.forename: Nam Soo
authors.initials: NS
authors.lastname: Han
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods; D003013:Clostridium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005285:Fermentation; D006632:Histamine / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000382:microbiology*; D014439:Tyramine / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D006632:Histamine; D014439:Tyramine