title: Antioxidant properties of Baccharis trimera in the neutrophils of Fisher rats.
abstract: BACKGROUND
  Baccharis trimera (Less.) (Asteraceae) is a native plant of Brazil. Also known as "carqueja", it has been popularly used to treat liver diseases, diabetes, as well as digestive disorders. Other studies have described the hepatoprotective, antioxidant and anti-inflammatory activities of the species.
  
  
  OBJECTIVE
  The aim of the present study was to investigate the antioxidant properties of Baccharis trimera in the neutrophils of Fisher rats in both in vitro and in vivo experimental models.
  
  
  METHODS
  In the in vitro assay, the neutrophils of male rats were isolated and incubated with Baccharis trimera extract at concentrations of 0.5, 5.0 and 50.0 microg/mL. In the in vivo assay, male rats were first treated with crude extract 600 mg/kg body weight of Baccharis trimera or with 50 mg/kg body weight of quercetin (reference substance) and then treated with 835 mg/kg of acetaminophen (APAP) after 24 h.
  
  
  RESULTS
  The hydroethanolic extract of Baccharis trimera reduced the release of reactive oxygen species in the neutrophils in both the in vitro and in vivo experimental models. Therefore confirming its antioxidant effect.
  
  
  CONCLUSIONS
  The results of this study confirm the antioxidant effect of Baccharis trimera.
authors.affiliation: Programa de Pós-graduação em Ciências Biológicas do Núcleo de Pesquisas em Ciências Biológicas-NUPEB, Universidade Federal de Ouro Preto (UFOP), Ouro Preto, MG, Brazil.
authors.forename: Bruno da Cruz
authors.initials: Bda C
authors.lastname: Pádua
authors.forename: Lucas Dornela
authors.initials: LD
authors.lastname: Silva
authors.forename: Joamyr Victor
authors.initials: JV
authors.lastname: Rossoni Júnior
authors.forename: Jorge Luiz
authors.initials: JL
authors.lastname: Humberto
authors.forename: Míriam Martins
authors.initials: MM
authors.lastname: Chaves
authors.forename: Marcelo Eustáquio
authors.initials: ME
authors.lastname: Silva
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Pedrosa
authors.forename: Daniela Caldeira
authors.initials: DC
authors.lastname: Costa
mesh_terms: D000082:Acetaminophen / Q000494:pharmacology; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D031175:Baccharis / Q000737:chemistry*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013997:Time Factors; D015054:Zymosan / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D000082:Acetaminophen; D015054:Zymosan; D011794:Quercetin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase