title: The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation.
abstract: CD4(+) helper T cells acquire effector phenotypes that promote specialized inflammatory responses. We show that the ETS-family transcription factor PU.1 was required for the development of an interleukin 9 (IL-9)-secreting subset of helper T cells. Decreasing PU.1 expression either by conditional deletion in mouse T cells or the use of small interfering RNA in human T cells impaired IL-9 production, whereas ectopic PU.1 expression promoted IL-9 production. Mice with PU.1-deficient T cells developed normal T helper type 2 (T(H)2) responses in vivo but showed attenuated allergic pulmonary inflammation that corresponded to lower expression of Il9 and chemokines in peripheral T cells and in lungs than that of wild-type mice. Together our data suggest a critical role for PU.1 in generating the IL-9-producing (T(H)9) phenotype and in the development of allergic inflammation.
authors.affiliation: Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Hua-Chen
authors.initials: HC
authors.lastname: Chang
authors.forename: Sarita
authors.initials: S
authors.lastname: Sehra
authors.forename: Ritobrata
authors.initials: R
authors.lastname: Goswami
authors.forename: Weiguo
authors.initials: W
authors.lastname: Yao
authors.forename: Qing
authors.initials: Q
authors.lastname: Yu
authors.forename: Gretta L
authors.initials: GL
authors.lastname: Stritesky
authors.forename: Rukhsana
authors.initials: R
authors.lastname: Jabeen
authors.forename: Carl
authors.initials: C
authors.lastname: McKinley
authors.forename: Ayele-Nati
authors.initials: AN
authors.lastname: Ahyi
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.forename: Evelyn T
authors.initials: ET
authors.lastname: Nguyen
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Robertson
authors.forename: Narayanan B
authors.initials: NB
authors.lastname: Perumal
authors.forename: Robert S
authors.initials: RS
authors.lastname: Tepper
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Nutt
authors.forename: Mark H
authors.initials: MH
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D006967:Hypersensitivity; D007249:Inflammation; D016906:Interleukin-9 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011518:Proto-Oncogene Proteins / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology*; D015534:Trans-Activators / Q000276:immunology*; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D016906:Interleukin-9; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1