The effect of TAT conjugated platinum nanoparticles on lifespan in a nematode Caenorhabditis elegans model.
We have shown that platinum nanoparticle species (nano-Pt) is a superoxide dismutase/catalase mimetic that scavenges superoxide and hydrogen peroxide. In Caenorhabditis elegans, nano-Pt functions as an effective antioxidant that induces an extension in lifespan and strong resistance against excessive oxidative stress. Our study with C. elegans was the first trial to use nano-Pt as a bio-active substance. However, a high concentration of nano-Pt was required for these survival effects, probably due to limited membrane permeability. Here, we show that the conjugation of nano-Pt with an HIV-1 TAT fusion protein C-terminally linked to a peptide with high affinity for platinum improves internalization, eliciting a similar level of antioxidant effects at one hundredth the concentration of unconjugated nano-Pt. This approach is a potential method to facilitate translocation of bio-active nanoparticles into living organisms and could be a model assay for estimate the effects of antioxidant in living organism.
Kim
Juewon
J
Department of Integrated Biosciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
Shirasawa
Takuji
T
Miyamoto
Yusei
Y
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000494:pharmacology; D040761:Biomimetic Materials / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D017173:Caenorhabditis elegans / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry; D015497:HIV-1 / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010984:Platinum / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000494:pharmacology; D013499:Surface Properties; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry*
D000975:Antioxidants; D004337:Drug Carriers; D054322:tat Gene Products, Human Immunodeficiency Virus; D010984:Platinum