Decreased serine207 phosphorylation of troponin T as a biomarker for left ventricular remodelling after myocardial infarction.
OBJECTIVE
Chronic heart failure following myocardial infarction (MI) is characterized by progressive left ventricular remodelling (LVR). Despite significant improvements in MI management, LVR remains a frequent complication. Although several risk factors have been identified, such as infarct size, LVR is difficult to predict in clinical practice.


RESULTS
Using a rat model of MI and phosphoproteomic technology, we discovered that remodelling is associated with decreased levels of myocardial and plasma serine(208)-phosphorylated troponin T (TnT). To confirm the association in human plasma, we developed new specific polyclonal antibodies against human/rat serine(207/208)-phosphorylated TnT and tested plasma obtained in the first week after MI from patients with low, intermediate, and high remodelling a year later. We observed a significant decrease of serine(207)-phosphorylated TnT and of the serine(207)-phosphorylated TnT/total TnT ratio in those with intermediate or high LVR. These differences remained statistically significant when adjusted for other determinants of LVR. In contrast, baseline B-type natriuretic peptide levels were not associated with LVR.


CONCLUSIONS
The level of circulating phosphorylated TnT could be a new biomarker of LVR.
Dubois
Emilie
E
INSERM U744, Lille, France.
Richard
Vincent
V
Mulder
Paul
P
Lamblin
Nicolas
N
Drobecq
Hervé
H
Henry
Jean-Paul
JP
Amouyel
Philippe
P
Thuillez
Christian
C
Bauters
Christophe
C
Pinet
Florence
F
D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000378:metabolism; D002908:Chronic Disease; D006333:Heart Failure / Q000473:pathology*; D006801:Humans; D008297:Male; D009203:Myocardial Infarction / Q000473:pathology*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D020107:Troponin T / Q000276:immunology / Q000378:metabolism*; D020257:Ventricular Remodeling / Q000502:physiology*
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D020107:Troponin T