Evidence that highly conserved residues of transmembrane segment 6 of Escherichia coli MntH are important for transport activity.
Nramp (natural resistance-associated macrophage protein) family members have been characterized in mammals, yeast, and bacteria as divalent metal ion/H(+) symporters. In previous work, a bioinformatic approach was used for the identification of residues that are conserved within the Nramp family [Haemig, H. A., and Brooker, R. J. (2004) J. Membr. Biol. 201 (2), 97-107]. On the basis of site-directed mutagenesis of highly conserved negatively charged residues, a model was proposed for the metal binding site of the Escherichia coli homologue, MntH. In this study, we have focused on the highly conserved residues, including two histidines, of transmembrane segment 6 (TMS-6). Multiple mutants were made at the eight conserved sites (i.e., Gly-205, Ala-206, Met-209, Pro-210, His-211, Leu-215, His-216, and Ser-217) in TMS-6 of E. coli MntH. Double mutants involving His-211 and His-216 were also created. The results indicate the side chain volume of these residues is critically important for function. In most cases, only substitutions that are closest in side chain volume still permit transport. In addition, the K(m) for metal binding is largely unaffected by mutations in TMS-6, whereas V(max) values were decreased in all mutants characterized kinetically. Thus, these residues do not appear to play a role in metal binding. Instead, they may comprise an important face on TMS-6 that is critical for protein conformational changes during transport. Also, in contrast to other studies, our data do not strongly indicate that the conserved histidine residues play a role in the pH regulation of metal transport.
Haemig
Heather A H
HA
Department of Genetics, Cell Biology, and Development, University of Minnesota, 321 Church Street, Minneapolis, Minnesota 55455, USA.
Moen
Patrick J
PJ
Brooker
Robert J
RJ
D000595:Amino Acid Sequence; D027682:Cation Transport Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D017124:Conserved Sequence / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D006639:Histidine / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D017433:Protein Structure, Secondary / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D021381:Protein Transport / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity / Q000235:genetics; D000099173:Natural Resistance-associated Macrophage Protein 1
D027682:Cation Transport Proteins; D029968:Escherichia coli Proteins; D006639:Histidine; D000099173:Natural Resistance-associated Macrophage Protein 1; C492412:MntH protein, E coli