title: Enhancing chemotherapeutic drug inhibition on tumor growth by ultrasound: an in vivo experiment.
abstract: An in vivo study on enhancing epirubicin hydrochloride (EPI) inhibition on tumor growth by ultrasound (US) was reported. Five-week-old male nude mice were used and HL-60 cells were s.c. (subcutaneous injection) inoculated in axilla of these mice. Six groups were designed and five consecutive treatments were applied to investigate the inhibition on tumor growth and body weight growth. US applied locally to the tumor resulted in a substantially increased drug uptake in tumor cells. The inhibition on tumor growth depended on the position of drug injection and phospholipid-based microbubble (PMB) application. Tumor growth rate under group 1 (PMB+US) was similar to that of blank control. The order of the inhibition on tumor volume growth was: group 4 (s.c. EPI+PMB+US) > group 5 intraperitoneal (i.p. EPI+PMB+US) > group 2 (i.p. EPI) > group 3 (s.c. EPI+US) > group 1 (PMB+US). Similar conclusion was obtained from experimental measurements of tumor weight change. The order of animal survival status for EPI administration groups was: group 4 > group 5 > group 2 > group 3. Chemotherapeutic drug inhibition on tumor growth could be enhanced by local US combined with PMB, which might provide a potential application for US-mediated chemotherapy.
authors.affiliation: Pharmacy School, Wenzhou Medical College, Zhejiang Province 325035, China. lctuua@yahoo.com.cn
authors.forename: Ying-Zheng
authors.initials: YZ
authors.lastname: Zhao
authors.forename: Cui-Tao
authors.initials: CT
authors.lastname: Lu
authors.forename: Zhi-Cai
authors.initials: ZC
authors.lastname: Zhou
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Jin
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Chang-Zheng
authors.initials: CZ
authors.lastname: Sun
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Xu
authors.forename: Hui-Sheng
authors.initials: HS
authors.lastname: Gao
authors.forename: Ji-Lai
authors.initials: JL
authors.lastname: Tian
authors.forename: Feng-Hou
authors.initials: FH
authors.lastname: Gao
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Tang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiang
authors.forename: Xiao-Kun
authors.initials: XK
authors.lastname: Li
authors.forename: Wen-Feng
authors.initials: WF
authors.lastname: Li
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D016503:Drug Delivery Systems; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D015251:Epirubicin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D045423:Microbubbles; D010743:Phospholipids / Q000737:chemistry; D013534:Survival; D014465:Ultrasonics / Q000379:methods*
chemical_list: D000903:Antibiotics, Antineoplastic; D010743:Phospholipids; D015251:Epirubicin