title: Multicenter collaborative study for standardization of Candida albicans genotyping using a polymorphic microsatellite marker.
abstract: Microsatellite-based genotyping for Candida albicans can give discrepant results between laboratories when expressed in fragment sizes, because their determination depends on electrophoretic conditions. The interlaboratory reproducibility was assessed in six laboratories provided with an allelic ladder. Despite variations in size determinations, alleles were correctly assigned, making data transportable between laboratories.
authors.affiliation: Institut Pasteur, Unité de Mycologie Moléculaire, Centre National de Référence Mycologie et Antifongiques and CNRS URA3012, Paris, France. dea.garcia-hermoso@pasteur.fr
authors.forename: Dea
authors.initials: D
authors.lastname: Garcia-Hermoso
authors.forename: Donna M
authors.initials: DM
authors.lastname: MacCallum
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Lott
authors.forename: Paula
authors.initials: P
authors.lastname: Sampaio
authors.forename: Maria-José Buitrago
authors.initials: MJ
authors.lastname: Serna
authors.forename: Fréderic
authors.initials: F
authors.lastname: Grenouillet
authors.forename: Corné H W
authors.initials: CH
authors.lastname: Klaassen
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bretagne
mesh_terms: D000483:Alleles; D015373:Bacterial Typing Techniques / Q000379:methods / Q000592:standards; D002176:Candida albicans / Q000235:genetics*; D005838:Genotype; D007753:Laboratories; D018895:Microsatellite Repeats / Q000235:genetics*; D025202:Molecular Diagnostic Techniques / Q000379:methods / Q000592:standards; D011110:Polymorphism, Genetic; D012015:Reference Standards; D015203:Reproducibility of Results