title: Site-directed mutagenesis in the study of membrane transporters.
abstract: One of the major goals in membrane transporter research is to understand how transporter proteins work at the molecular level. Ideally, this research would be carried out with a detailed knowledge of the three-dimensional structure of the protein. However, in the absence of atomic resolution structures for many membrane transporters other molecular tools need to be employed. In vitro site-directed mutagenesis is one method that has the capacity to provide both structural information and identification of the role of individual residues and/or regions of a protein that are involved in function.
authors.affiliation: Transporter Biology Group, Discipline of Pharmacology and Bosch Institute, University of Sydney, Sydney, NSW, Australia.
authors.forename: Audra A
authors.initials: AA
authors.lastname: McKinzie
authors.forename: Renae M
authors.initials: RM
authors.lastname: Ryan
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Vandenberg
mesh_terms: D000818:Animals; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D026901:Membrane Transport Proteins; D011993:Recombinant Fusion Proteins