title: Differential roles of leptin in regulating cell migration in thyroid cancer cells.
abstract: Excess body weight is associated with a moderately increased risk of thyroid cancer. Adipocyte-derived hormone, leptin, has been shown to enhance cell growth and migration in many cancer types. Limited evidence suggests that leptin has direct actions on the thyroid gland, but there are no data available on the effect of leptin on thyroid cancer cells. We evaluated the action of leptin on gene expression, cell growth, cell cycle, and cell migration in anaplastic (ARO), follicular (WRO) and papillary (CGTH-W3) thyroid carcinoma cell lines. Expression of long-form leptin receptors was observed in all thyroid cancer cell lines. Leptin stimulation did not alter the expression levels of leptin, leptin receptor and sodium-iodide symporter. Cell growth and cell cycle were not changed after leptin treatment. However, leptin was able to promote cell migration of papillary thyroid cancer cells, but inhibited migration of anaplastic and follicular cancer cells. In summary, our study suggests that leptin modulates cell migration of thyroid cancer cells in a cell type-specific manner.
authors.affiliation: Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
authors.forename: Shih-Ping
authors.initials: SP
authors.lastname: Cheng
authors.forename: Pen-Hui
authors.initials: PH
authors.lastname: Yin
authors.forename: Yuan-Ching
authors.initials: YC
authors.lastname: Chang
authors.forename: Chen-Hsen
authors.initials: CH
authors.lastname: Lee
authors.forename: Shih-Yuan
authors.initials: SY
authors.lastname: Huang
authors.forename: Chin-Wen
authors.initials: CW
authors.lastname: Chi
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000378:metabolism* / Q000473:pathology; D017209:Apoptosis; D015153:Blotting, Western; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D002291:Carcinoma, Papillary / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D020738:Leptin / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D054411:Receptors, Leptin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D020738:Leptin; D012333:RNA, Messenger; D054411:Receptors, Leptin