Listeria monocytogenes efficiently invades Caco-2 cells after low-temperature storage in broth and on deli meat.
The objective of this study was to investigate how various growth conditions influence the virulence of Listeria monocytogenes monitored by its ability to invade the epithelial cell lines Caco-2 and INT-407. The growth conditions examined were modified atmosphere-packaged deli meat and brain heart infusion broth (BHI) with and without salt. Five strains of L. monocytogenes were selected to investigate their invasiveness and all strains invaded Caco-2 cells at higher levels than INT-407 cells. Further, the clinical strains (3443 and 3734) were more invasive (p < 0.05) than the strains isolated from meat and food-processing environments (3008, 3126, and 4140) after grown in BHI at 30 degrees C. This attenuation could not be ascribed to a defective Internalin A as all strains encoded an intact inlA gene. To determine the influence of food products on virulence, the ability of L. monocytogenes to invade Caco-2 cells was compared after growth on a fermented sausage and on cured cooked ham to that of bacteria grown in BHI broth supplemented with salt. Samples were stored under chilling conditions for up to 4 weeks. The results showed no difference (p > 0.05) in invasiveness after 7 days at 10 degrees C in BHI broth or on sausage, whereas a slight increase (p < 0.05) was observed after incubation on ham for 2 and 4 weeks compared to that in BHI broth. Most importantly, our results show that L. monocytogenes efficiently invade Caco-2 cells even after 4 weeks of storage at chilled temperature. This is highly relevant for safety assessment of this organism in food as these conditions reflect storage of ready-to-eat food products in domestic refrigerators.
Larsen
Marianne Halberg
MH
Department of Veterinary Disease Biology, Centre for Advanced Food Studies, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark. mhl@life.ku.dk
Koch
Anette Granly
AG
Ingmer
Hanne
H
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques; D018938:Caco-2 Cells; D002460:Cell Line; D003080:Cold Temperature; D003470:Culture Media; D004847:Epithelial Cells / Q000382:microbiology; D005511:Food Handling; D005516:Food Microbiology; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008089:Listeria monocytogenes / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D008088:Listeriosis / Q000382:microbiology; D008460:Meat / Q000382:microbiology; D008461:Meat Products / Q000382:microbiology; D013045:Species Specificity
D001426:Bacterial Proteins; D003470:Culture Media; C069761:internalin protein, Bacteria