title: 5.4 nm spatial resolution in biological photoemission electron microscopy.
abstract: We report a spatial resolution of 5.4 nm in images of sarcoplasmic reticulum from rabbit muscle. The images were obtained in an aberration-corrected photoemission electron microscope with a hyperbolic mirror as the correcting element for spherical and chromatic aberration. In-situ measurements and numerical simulations confirm the low residual aberration in the instrument and indicate the ultimate resolution in this type of microscopy to be below 2 nm.
authors.affiliation: Physics Department, Portland State University, 1719 SW 10th Avenue, Portland, OR 97201, USA. rkoe@pdx.edu
authors.forename: R
authors.initials: R
authors.lastname: Könenkamp
authors.forename: Robert C
authors.initials: RC
authors.lastname: Word
authors.forename: G F
authors.initials: GF
authors.lastname: Rempfer
authors.forename: T
authors.initials: T
authors.lastname: Dixon
authors.forename: L
authors.initials: L
authors.lastname: Almaraz
authors.forename: T
authors.initials: T
authors.lastname: Jones
mesh_terms: D000818:Animals; D004867:Equipment Design; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure