title: Detection of human papillomaviruses type 16, 18 and 33 in bronchial aspirates of lung carcinoma patients by polymerase chain reaction: a study of 84 cases in Croatia.
abstract: Besides its well-known role in cervical carcinoma, HPV is also suggested to be involved in lung cancer development. A number of authors have been investigating the presence of HPV in histological materials. We used routine bronchial aspirates from 84 patients with lung carcinoma for DNA extraction and then performed polymerase chain reaction for high-risk HPV types 16, 18 and 33. The results were compared to those obtained from buccal and eyelid mucosa. Only three patients were positive for HPV in bronchial aspirates: one for HPV 16 type, one for HPV 18 type, and one for HPV 33. Our data indicated the low prevalence of HPV in patients with lung carcinomas in Croatia, therefore it seems unlikely that HPV contributes to the development of lung carcinomas in this region.
authors.affiliation: Department of Cytology, University Hospital for Lung Diseases "Jordanovac", Zagreb, Croatia. bozica.vrabec.branica@zg.t-com.hr
authors.forename: Bozica Vrabec
authors.initials: BV
authors.lastname: Branica
authors.forename: Silvana
authors.initials: S
authors.lastname: Smojver-Jezek
authors.forename: Zrinka
authors.initials: Z
authors.lastname: Juros
authors.forename: Sandra
authors.initials: S
authors.lastname: Grgić
authors.forename: Nives
authors.initials: N
authors.lastname: Srpak
authors.forename: Dinko
authors.initials: D
authors.lastname: Mitrecić
authors.forename: Srećko
authors.initials: S
authors.lastname: Gajović
mesh_terms: D000230:Adenocarcinoma / Q000453:epidemiology / Q000473:pathology / Q000821:virology; D000368:Aged; D000369:Aged, 80 and over; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D018287:Carcinoma, Large Cell / Q000453:epidemiology / Q000473:pathology / Q000821:virology; D018288:Carcinoma, Small Cell / Q000453:epidemiology / Q000473:pathology / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000473:pathology / Q000821:virology; D017523:Croatia / Q000453:epidemiology; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000453:epidemiology*; D016133:Polymerase Chain Reaction; D015995:Prevalence
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral