title: Chemoenzymatic synthesis of the sialyl Lewis X glycan and its derivatives.
abstract: A combination of recombinant FKP and alpha-(1-->3)-fucosyltransferase allows the facile synthesis of the sialyl Lewis X tetrasaccharide glycan and its derivatives in excellent yield. In this system, the universal fucosyl donor, guanidine 5'-diphosphate-beta-L-fucose (GDP-fucose), or its analogues can be generated in situ by cofactor recycling using pyruvate kinase.
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461, USA.
authors.forename: David
authors.initials: D
authors.lastname: Soriano del Amo
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Christen
authors.initials: C
authors.lastname: Besanceney
authors.forename: Tianqing
authors.initials: T
authors.lastname: Zheng
authors.forename: Yizheng
authors.initials: Y
authors.lastname: He
authors.forename: Brian
authors.initials: B
authors.lastname: Gerwe
authors.forename: Ronald D
authors.initials: RD
authors.lastname: Seidel
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
mesh_terms: D000606:Amino Sugars / Q000737:chemistry / Q000378:metabolism; D002240:Carbohydrate Sequence; D005643:Fucose / Q000737:chemistry / Q000378:metabolism; D005647:Fucosyltransferases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000378:metabolism*; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D000080506:Sialyl Lewis X Antigen; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D000606:Amino Sugars; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; C032233:lactosamine; D005643:Fucose; D005647:Fucosyltransferases; C115708:GDP-L-fucose pyrophosphorylase; D009713:Nucleotidyltransferases