Inhibitory effect of Crocus sativus (saffron) on histamine (H1) receptors of guinea pig tracheal chains.
The inhibitory effects of aqueous-ethanolic extracts of Crocus sativus (Iridaceae), on histamine (H1) receptors was examined on tracheal chains of guinea pigs. The effects of three concentrations of aqueous-ethanolic extract, 10 nM chlorpheniramine, and saline on histamine (H1) receptors were tested on three groups of guinea pig tracheal chains as follows; incubated trachea with: 1) indomethacin, 2) indomethacin, propranolol, and atropine and 3) indomethacin and propranolol. The EC50 (effective concentration of histamine causing 50% of maximum response) obtained in the presence of chlorpheniramine and all concentrations of the extract in all three groups were significantly greater than those of saline (p<0.05 to p<0.001) except low concentration of the extract in groups 1 and 3. The EC50 obtained in the presence of two higher concentrations of extract in group 2 were greater than group 1 and 3 (p<0.05 to p<0.001). Maximum response obtained in the presence of two higher concentrations of extract in group 2 were greater than those of group 1 and group 3 (p<0.001 for all cases). There were parallel right ward shift in concentration response curves obtained in the presence of only low and medium concentrations of the extract in group 2 compared to the those of saline. These results indicated an inhibitory effect of Crocus sativus at histamine H1 receptors.
Boskabady
M H
MH
Departement of Physiology and Pharmacological Research Centre of Medicinal Plants, Medical School Mashhad University of Medical Sciences, Mashhad, Iran. boskabadymh@mums.ac.ir
Ghasemzadeh Rahbardar
M
M
Nemati
H
H
Esmaeilzadeh
M
M
D000465:Algorithms; D000818:Animals; D002744:Chlorpheniramine / Q000494:pharmacology; D027622:Crocus / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011969:Receptors, Histamine H1 / Q000187:drug effects*; D014132:Trachea / Q000187:drug effects*; D014867:Water
D006634:Histamine H1 Antagonists; D010936:Plant Extracts; D011969:Receptors, Histamine H1; D014867:Water; D000431:Ethanol; D002744:Chlorpheniramine; D006632:Histamine