Daidzein does affect progesterone secretion by pig cumulus cells but it does not impair oocytes IVM.
Daidzein, an isoflavone abundant in soybeans and other legumes, displays estrogen like properties. This study was aimed at evaluating the effect of daidzein (1 and 10 microM) on nuclear and cytoplasmic maturation of pig oocytes and on steroidogenic activity of cumulus cells. Daidzein supplementation during IVM had no effect on nuclear maturation and on fertilization traits. By contrast, both concentrations significantly (P < 0.05) inhibited progesterone production by cumulus cells after 24 and 48 h of culture while they did not induce any effect on estradiol production. Furthermore, daidzein did not exert any effect on the percentage of embryos that developed to blastocyst stage, on the number of blastomeres per blastocyst, or on the level of Hsp-70 and -90 gene transcript. Overall, our data demonstrate that daidzein added during oocyte maturation does not affect pig embryo development even if it markedly inhibits progesterone production by cumulus cells. Further studies are needed to evaluate the possible effect of daidzein during embryonic development.
Galeati
Giovanna
G
Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Università di Bologna, Via Tolara di Sopra, 50, 40064 Ozzano Emilia (BO), Italia. giovanna.galeati@unibo.it
Vallorani
Claudia
C
Bucci
Diego
D
Bernardini
Chiara
C
Tamanini
Carlo
C
Parmeggiani
Albamaria
A
Spinaci
Marcella
M
D000818:Animals; D054885:Cumulus Cells / Q000187:drug effects* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D011374:Progesterone / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D007529:Isoflavones; D012333:RNA, Messenger; D011374:Progesterone; C004742:daidzein