SNL fibroblast feeder layers support derivation and maintenance of human induced pluripotent stem cells.
Induced pluripotent stem (iPS) cells can be derived from human somatic cells by cellular reprogramming. This technology provides a potential source of non-controversial therapeutic cells for tissue repair, drug discovery, and opportunities for studying the molecular basis of human disease. Normally, mouse embryonic fibroblasts (MEFs) are used as feeder layers in the initial derivation of iPS lines. The purpose of this study was to determine whether SNL fibroblasts can be used to support the growth of human iPS cells reprogrammed from somatic cells using lentiviral expressed reprogramming factors. In our study, iPS cells expressed common pluripotency markers, displayed human embryonic stem cells (hESCs) morphology and unmethylated promoters of NANOG and OCT4. These data demonstrate that SNL feeder cells can support the derivation and maintenance of human iPS cells.
Pan
Chuanying
C
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, China.
Hicks
Amy
A
Guan
Xuan
X
Chen
Hong
H
Bishop
Colin E
CE
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000502:physiology
D050814:Octamer Transcription Factor-3