Elimination and detoxification of triclosan by manganese peroxidase from white rot fungus.
The antimicrobial and preservative agent triclosan (TCS) is an emerging and persistent pollutant with a ubiquitous presence in the aquatic environment. Thus, TCS was treated with manganese peroxidase (MnP), laccase and the laccase-mediator system with 1-hydroxybenzotriazole. MnP was most effective in eliminating TCS among the three enzymatic treatments, with TCS concentration being reduced by about 94% after 30 min following treatment with 0.5 nkat mL(-1) MnP and being almost completely eliminated after 60 min. Furthermore, MnP (0.5 nkat mL(-1)) caused the complete loss of bacterial growth inhibition by TCS after 30 min and reduced the algal growth inhibition of TCS by 75% and 90% after 30 and 60 min, respectively. These results strongly suggest that MnP is effective in removing the ecotoxicity of TCS.
Inoue
Yukiko
Y
Faculty of Agriculture, Shizuoka University, Surugaku, Shizuoka 422-8529, Japan.
Hata
Takayuki
T
Kawai
Shingo
S
Okamura
Hideo
H
Nishida
Tomoaki
T
D000890:Anti-Infective Agents / Q000378:metabolism*; D001419:Bacteria / Q000254:growth & development; D001487:Basidiomycota / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D042845:Laccase / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D014260:Triclosan / Q000378:metabolism*
D000890:Anti-Infective Agents; D014260:Triclosan; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase