[pCTLA4-Ig gene-modified porcine immature dendritic cells inhibit the proliferation of xenogeneic spleen cells].
OBJECTIVE
To establish a method for induced and amplified porcine monocyte-derived immature dendritic cells(imDCs).The aim of this study was to assess the pCTLA4-Ig gene-modified porcine imDCs could benefit for prevent the proliferation of xenogeneic spleen cells.


METHODS
Poricne peripheral blood mononuclear cells(PBMC) were cultured with rpGM- CSF(30 microg/L) and rpIL-4(20 microg/L). To evaluate the suspending cells by transmission electronic microscope and flow cytometer. Porcine imDCs were transduced with adenovirus mediated pCTLA4-Ig gene. The mixed lymphocyte reaction(MLR) was used to examine the proliferation of spleen cells of SD rat to gene-modified imDCs.


RESULTS
With rpGM-CSF and rpIL-4 induced porcine PBMC for 4-5 days, it was exhibited typical morphological characteristics and immunological phenotype of imDCs with high expression of SLA-DR, CD172a and low expression of CD80/CD86 on the cellular surface. The gene-modified imDCs were able to amplified the pCTLA4-Ig and IDO gene by RT-PCR. The stimulate index of gene-modified imDCs group were markedly inferior to that in unmodified imDCs group(P<0.05).


CONCLUSIONS
The pCTLA4-Ig gene-modified imDCs can significantly inhibit the proliferation of xenogeneic spleen cells, and there might was correlated with the expression of IDO reduce the immune response of xenogeneic antigen.
Tian
Min
M
Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
Lü
Yi
Y
Zhai
Chao
C
Zhu
Hai-tao
HT
Wang
Bo
B
Yu
Liang
L
Zhang
Xiao-gang
XG
D000069594:Abatacept; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D005818:Genetic Engineering; D006801:Humans; D018796:Immunoconjugates / Q000235:genetics / Q000378:metabolism*; D007959:Lymphocyte Culture Test, Mixed; D046529:Microscopy, Electron, Transmission; D051381:Rats; D013154:Spleen / Q000166:cytology* / Q000276:immunology / Q000637:transplantation; D013552:Swine; D014162:Transfection; D014183:Transplantation, Heterologous
D018796:Immunoconjugates; D000069594:Abatacept