title: Effects of isolated compounds from Catalpa ovata on the T cell-mediated immune responses and proliferation of leukemic cells.
abstract: Three compounds were isolated from the ethyl acetate soluble fraction of the methanolic extract of the leaves of Catalpa ovata (Bignoniaceae) through repeated column chromatography. We investigated the effects of these compounds on T cell-mediated responses for tumor surveillance and proliferation in U937, HL60, and Molt-4 leukemia cells. Compounds 1-3 inhibited proliferation of those cells in a dose-dependent manner. Compound 3 showed mild effect in Molt-4 cell cytotoxicity. Compound 3 enhanced gene expressions of p53 and IL-4, but decreased IL-2 and IFN-Gamma genes in Molt-4 cell. Our findings indicate that compound 3 may enhance T cell-mediated immune responses and anticancer properties.
authors.affiliation: Department of Food & Biotechnology, Woosuk University, Jeonju, 565-701, Korea.
authors.forename: Chan-Ho
authors.initials: CH
authors.lastname: Oh
authors.forename: Nam-Seok
authors.initials: NS
authors.lastname: Kim
authors.forename: Jae Heon
authors.initials: JH
authors.lastname: Yang
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Lee
authors.forename: Seokwon
authors.initials: S
authors.lastname: Yang
authors.forename: Saerom
authors.initials: S
authors.lastname: Park
authors.forename: Un-Ki
authors.initials: UK
authors.lastname: So
authors.forename: Jin-Beom
authors.initials: JB
authors.lastname: Bae
authors.forename: Jae Soon
authors.initials: JS
authors.lastname: Eun
authors.forename: Hoon
authors.initials: H
authors.lastname: Jeon
authors.forename: Jong Pil
authors.initials: JP
authors.lastname: Lim
authors.forename: Jin
authors.initials: J
authors.lastname: Kwon
authors.forename: Young-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Tae Yong
authors.initials: TY
authors.lastname: Shin
authors.forename: Dae Keun
authors.initials: DK
authors.lastname: Kim
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D029402:Bignoniaceae / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects* / Q000276:immunology; D055670:Medicine, Korean Traditional; D015394:Molecular Structure; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D020298:U937 Cells
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016207:Cytokines; D010936:Plant Extracts