title: Hantavirus-like particles generated in CHO cells induce specific immune responses in C57BL/6 mice.
abstract: A safe and effective hantavirus vaccine is highly desirable since hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, HFRS). Virus-like particles (VLPs) displaying functional viral proteins could provide effective vaccines against a few viruses, but their ability to induce hantavirus-specific immune response has not been adequately investigated. To measure the immunogenicity of Hantaan virus-like particles (HTN-VLPs) vaccine, we generated recombinant HTN-VLPs by co-expressing Hantaan virus nucleocapsid (N) protein and glycoproteins (Gn and Gc) in Chinese hamster ovary (CHO) cells. We compared intramuscular versus subcutaneous administration of HTN-VLPs for the ability to induce specific immune response against Hantaan virus infection. Mice that received both intramuscular and subcutaneous immunizations of HTN-VLPs were sufficiently stimulated specific antibody response against Hantaan virus N protein and glycoproteins, which was comparable to Chinese commercial inactivated bivalent hantaviruses vaccine. Moreover, vaccination with HTN-VLPs also resulted in the induction of higher levels of specific cellular response to N protein than that of inactivated vaccine. Our results provide an important insight towards the development of hantaviruses-like particles as a potential candidate vaccine for the control and prevention of hantaviruses infection.
authors.affiliation: State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC 100 Ying Xin Jie, Xuan Wu Qu, Beijing 100052, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Mifang
authors.initials: M
authors.lastname: Liang
authors.forename: Quanfu
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Jiandong
authors.initials: J
authors.lastname: Li
authors.forename: Dexin
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016466:CHO Cells; D036022:Capsid Proteins / Q000276:immunology; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D018063:Hantaan virus / Q000254:growth & development / Q000276:immunology*; D018778:Hantavirus Infections / Q000276:immunology / Q000517:prevention & control*; D007074:Immunoglobulin G / Q000097:blood; D007273:Injections, Intramuscular; D007279:Injections, Subcutaneous; D051379:Mice; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D014709:Vero Cells; D014758:Viral Core Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D014771:Virion / Q000254:growth & development* / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; D014758:Viral Core Proteins; D014765:Viral Vaccines; C111782:nucleocapsid protein, Hantaan virus