hSHIP induces S-phase arrest and growth inhibition in cervical cancer HeLa cells.
hSHIP, a human SH2-containing inositol-5-phosphatase, acts as a negative regulator of proliferation and survival in hematopoietic cells. Therefore, hSHIP may play a crucial role in suppression of cervical cancer HeLa cells. In this study, pcDNA3.1-hSHIP-GFP plasmid was constructed and transfected into HeLa cells with Lipofectamine2000, stably transfected HeLa cells were established and their responses were investigated by Flow cytometry, MTT, tumorigenicity in nude mice, RT-PCR and ELISA assays. The results showed that the expression of hSHIP significantly induced S-phase arrest, cell growth inhibition, and down-regulation of Akt1/2 mRNA and p-Akt in HeLa cells. Our study supports an important role for hSHIP in suppression of cervical cancer HeLa cells, which may prove to be a novel therapeutic option for non-hematopoietic cancers.
He
Kangxia
K
School of Life Science, Xiamen University, 422 Siming South Road, Xiamen 361005, China.
He
Jie
J
Wang
Shengyu
S
Yan
Jianghua
J
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation; D005260:Female; D006131:Growth Inhibitors / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases; D010744:Phosphoric Monoester Hydrolases / Q000494:pharmacology*; D016196:S Phase / Q000187:drug effects*; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
D006131:Growth Inhibitors; D010744:Phosphoric Monoester Hydrolases; C098706:INPPL1 protein, human; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases