title: Decreased serine207 phosphorylation of troponin T as a biomarker for left ventricular remodelling after myocardial infarction.
abstract: OBJECTIVE
  Chronic heart failure following myocardial infarction (MI) is characterized by progressive left ventricular remodelling (LVR). Despite significant improvements in MI management, LVR remains a frequent complication. Although several risk factors have been identified, such as infarct size, LVR is difficult to predict in clinical practice.
  
  
  RESULTS
  Using a rat model of MI and phosphoproteomic technology, we discovered that remodelling is associated with decreased levels of myocardial and plasma serine(208)-phosphorylated troponin T (TnT). To confirm the association in human plasma, we developed new specific polyclonal antibodies against human/rat serine(207/208)-phosphorylated TnT and tested plasma obtained in the first week after MI from patients with low, intermediate, and high remodelling a year later. We observed a significant decrease of serine(207)-phosphorylated TnT and of the serine(207)-phosphorylated TnT/total TnT ratio in those with intermediate or high LVR. These differences remained statistically significant when adjusted for other determinants of LVR. In contrast, baseline B-type natriuretic peptide levels were not associated with LVR.
  
  
  CONCLUSIONS
  The level of circulating phosphorylated TnT could be a new biomarker of LVR.
authors.affiliation: INSERM U744, Lille, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Dubois
authors.forename: Vincent
authors.initials: V
authors.lastname: Richard
authors.forename: Paul
authors.initials: P
authors.lastname: Mulder
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lamblin
authors.forename: Hervé
authors.initials: H
authors.lastname: Drobecq
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Henry
authors.forename: Philippe
authors.initials: P
authors.lastname: Amouyel
authors.forename: Christian
authors.initials: C
authors.lastname: Thuillez
authors.forename: Christophe
authors.initials: C
authors.lastname: Bauters
authors.forename: Florence
authors.initials: F
authors.lastname: Pinet
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000378:metabolism; D002908:Chronic Disease; D006333:Heart Failure / Q000473:pathology*; D006801:Humans; D008297:Male; D009203:Myocardial Infarction / Q000473:pathology*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D020107:Troponin T / Q000276:immunology / Q000378:metabolism*; D020257:Ventricular Remodeling / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D020107:Troponin T