A phylogenetic study of Boletus section Boletus in Europe.
A phylogenetic study of the species in Boletus sect. Boletus was undertaken using the molecular markers ITS1-5.8S-ITS2 and GAPDH. Four well-supported lineages, one comprising Boletus edulis s.l., the others referring to B. aereus, B. reticulatus and B. pinophilus have been distinguished. The ML and MP trees of ITS showed remarkably low resolution within the B. edulis clade, and confirmed earlier published results, despite the use of samples from a wider geographical area and different hosts. The results of GAPDH demonstrate clearly that this low resolution must be ascribed to a low genetic variability with the B. edulis clade, and make clear that morphological and ecological characters have been overestimated within this species complex. Boletus edulis is therefore defined as a variable species with a wide morphological, ecological and geographic range, and includes several specific and subspecific taxa described in the literature (e.g. B. betulicola, B. persoonii, B. quercicola and B. venturii). Three other European species (B. aereus, B. pinophilus and B. reticulatus) are well delimited species based on morphology and our genetic data.
Beugelsdijk
D C M
DC
National Herbarium of the Netherlands, Leiden University branch, P.O. Box 9514, 2300 RA Leiden, The Netherlands;
van der Linde
S
S
Zuccarello
G C
GC
den Bakker
H C
HC
Draisma
S G A
SG
Noordeloos
M E
ME
J Mol Evol. 1980 Dec;16(2):111-20
7463489
J Mol Evol. 1985;22(2):160-74
3934395
Bioinformatics. 2001 Aug;17(8):754-5
11524383
Mycologia. 2004 Jan-Feb;96(1):102-18
21148833
Bioinformatics. 1998;14(9):817-8
9918953
Mol Phylogenet Evol. 2002 Jun;23(3):357-400
12099793
Evolution. 1985 Jul;39(4):783-791
28561359
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
9396791
FEMS Microbiol Lett. 2005 Feb 15;243(2):411-6
15686843
New Phytol. 2004 Jul;163(1):201-215
33873790