[Effects of pravastatin on the proliferation and invasion of human hepatocarcinoma HepG2 cell line].
OBJECTIVE
To observe the effects of pravastatin on the proliferation and invasion of human hepatocarcinoma HepG2 cell line.


METHODS
The effects of pravastatin on the proliferation, migration and invasion of HepG2 cells was observed by MTT assay, Boyden chamber assay and motility assay. p38 activity was measured, and the expression of p-p38, MKP-1, RhoC and MMP-2 was analyzed by Western blot.


RESULTS
Pravastatin inhibited the proliferation of HepG2 cells. The intracellular p38 activity and expressions of p-p38, RhoC and MMP-2 were decreased, while MKP-1 expression was elevated in pravastatin treated cells. In addition, pravastatin inhibited the invasion and motility.


CONCLUSIONS
Pravastatin can inhibit the proliferation and invasion of HepG2 cells.
Zhang
Wen-jie
WJ
Department of Infectious Diseases, the Second Hospital of Gansu Province, Lanzhou, China. zhangwenjie718@163.com
Yang
Shao-hua
SH
Tian
Shu-ju
SJ
Li
Zhao-xia
ZX
Gong
Yan-xuan
YX
Qu
Yan
Y
Mao
Wei-wu
WW
D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D017035:Pravastatin / Q000494:pharmacology*
D020778:Matrix Metalloproteinase 2; D017035:Pravastatin