An antiproliferative ribonuclease from fruiting bodies of the wild mushroom Russula delica.
An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Russula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa as judged by fast protein liquid chromatography on Superdex 75 and SDS-polyacrylamide gel electrophoresis. Its optimal pH and optimal temperature were pH 5 and 60 degrees , respectively. The ranking of its activity toward various polyhomoribonucleotides was poly C > poly G > poly A > poly U. It could inhibit proliferation of HepG2 and MCF-7 cancer cells with an IC50 value of 8.6 microM and 7.2 microM, respectively. It was devoid of antifungal and HIV-1 reverse transcriptase inhibitory activity.
Zhao
Shuang
S
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193,China.
Zhao
Yongchang
Y
Li
Shuhong
S
Zhang
Guoqing
G
Wang
Hexiang
H
Ng
Tzi Bun
TB
D000363:Agaricales / Q000201:enzymology*; D000595:Amino Acid Sequence; D019380:Anti-HIV Agents / Q000302:isolation & purification / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048690:Fruiting Bodies, Fungal / Q000201:enzymology; D019427:HIV Integrase / Q000378:metabolism; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D012265:Ribonucleotides / Q000378:metabolism
D019380:Anti-HIV Agents; D000935:Antifungal Agents; D012265:Ribonucleotides; D019427:HIV Integrase; D054303:HIV Reverse Transcriptase; D012260:Ribonucleases