title: Growth hormone and two forms of insulin-like growth factors I in the giant grouper (Epinephelus lanceolatus): molecular cloning and characterization of tissue distribution.
abstract: Growth hormone and insulin-like growth factors play important roles in the growth, development and metabolism of vertebrates. In this study, we used reverse transcription and rapid amplification of cDNA ends (RACE) to obtain the three full-length cDNA sequences encoding GH and two forms of IGF-I from the giant grouper (Epinephelus lanceolatus), a coral fish of high commercial value cultured in Southeast Asia. GH precursor cDNA consists of 938 bp in size with an open-reading frame (ORF) encoding 204 amino acid (aa), a 65 bp 5'-untranslated region and a 236 bp 3'-untranslated region. The sequence of giant grouper GH shared 98.6% nucleotide sequence homology with orange-spotted grouper (E. coioides) GH. Two forms of IGF-I precursor cDNA were cloned from giant grouper, IGF-I a consisting of 159 aa, and IGF-I b with 186 aa. They shared 98.4 and 98.7% aa identity with IGF-I reported in the orange-spotted grouper, respectively. Giant grouper IGF-I a and b have the same signal peptide and B-C-A-D domains, but they are different in the E domain. Using real-time reverse transcription PCR strategy, tissue distribution profile showed that GH and IGF-I mRNA signals were all observed in pituitary, brain, liver, ovary and spleen. GH mRNA in pituitary was the most abundant, and IGF-I mRNA level in liver was found to be more abundant than that in other selected tissues. These findings will contribute to the understanding of the evolution of GH and IGF-I, and provide some basic information about the characterization of GH and IGF-I in the giant grouper.
authors.affiliation: State Key Laboratory of Biocontrol and Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Dong
authors.forename: Lingxian
authors.initials: L
authors.lastname: Zeng
authors.forename: Da
authors.initials: D
authors.lastname: Duan
authors.forename: Haifa
authors.initials: H
authors.lastname: Zhang
authors.forename: Yunxin
authors.initials: Y
authors.lastname: Wang
authors.forename: Wensheng
authors.initials: W
authors.lastname: Li
authors.forename: Haoran
authors.initials: H
authors.lastname: Lin
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism*; D013006:Growth Hormone / Q000235:genetics* / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D017931:DNA Primers; D018076:DNA, Complementary; D029941:Fish Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone
references.citation: Gen Comp Endocrinol. 2007 May 15;152(1):111-22
references.pmid: 17382945
references.citation: Gen Comp Endocrinol. 1999 Jan;113(1):155-64
references.pmid: 9882554
references.citation: Transgenic Res. 2007 Aug;16(4):479-89
references.pmid: 17431805
references.citation: J Biol Chem. 1978 Apr 25;253(8):2769-76
references.pmid: 632300
references.citation: Endocrinology. 2000 Aug;141(8):2886-94
references.pmid: 10919276
references.citation: Gen Comp Endocrinol. 1997 Apr;106(1):95-101
references.pmid: 9126469
references.citation: Endocrinology. 1995 Feb;136(2):446-52
references.pmid: 7835275
references.citation: Arch Biochem Biophys. 1996 Jun 1;330(1):19-23
references.pmid: 8651695
references.citation: Fish Physiol Biochem. 1993 Jul;11(1-6):363-9
references.pmid: 24202496
references.citation: Int Rev Cytol. 1998;183:1-94
references.pmid: 9666565
references.citation: Gen Comp Endocrinol. 2005 May 15;142(1-2):20-4
references.pmid: 15862544
references.citation: Neuroendocrinology. 2007;86(3):165-74
references.pmid: 17377370
references.citation: J Endocrinol. 2002 Oct;175(1):33-40
references.pmid: 12379488
references.citation: Gene. 2003 Dec 11;322:145-56
references.pmid: 14644506
references.citation: Mol Cell Endocrinol. 1998 Jun 25;141(1-2):187-94
references.pmid: 9723899
references.citation: Endocrinology. 1997 Sep;138(9):3613-9
references.pmid: 9275043
references.citation: Gen Comp Endocrinol. 1999 Jul;115(1):46-52
references.pmid: 10375463
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2007 Jun;147(2):325-39
references.pmid: 17341448
references.citation: Nature. 1994 Dec 1;372(6505):478-81
references.pmid: 7984244
references.citation: J Mol Endocrinol. 1996 Apr;16(2):123-32
references.pmid: 9156515
references.citation: Gen Comp Endocrinol. 2002 May;126(3):269-78
references.pmid: 12093114
references.citation: J Mol Endocrinol. 1994 Feb;12(1):25-37
references.pmid: 8185811
references.citation: Mol Reprod Dev. 1999 Jun;53(2):127-34
references.pmid: 10331450
references.citation: Gen Comp Endocrinol. 2005 Oct;144(1):78-89
references.pmid: 16055124
references.citation: Gen Comp Endocrinol. 2004 Jun;137(2):196-204
references.pmid: 15158131
references.citation: J Nutr. 1998 Feb;128(2 Suppl):306S-314S
references.pmid: 9478013