Small intestinal adenocarcinoma in common marmosets (Callithrix jacchus).
Small intestinal adenocarcinomas are uncommon neoplasms that are rarely reported in nonhuman primates. These neoplasms are also rare in humans, although they are thought to share a similar pathogenesis with the more common colorectal carcinoma. Herein the authors report the clinical, histologic, immunohistochemical, and molecular characteristics of small intestinal adenocarcinoma in 10 common marmosets (Callithrix jacchus). Retrospective analysis of necropsy records revealed small intestinal carcinoma to be the most common neoplastic cause of morbidity and mortality in aged common marmosets. The average age of affected animals was 6.6 years old, and there was no sex predilection. Nine of 10 (90%) tumors arose within the proximal small intestine near the interface with the duodenum. All cases were characterized by disorganization, loss of polarity, and proliferation of neoplastic epithelial cells along the crypt to midvillous interface. Two of 10 (20%) were defined as carcinoma in situ. Eight of 10 (80%) had some degree of invasion, with lymphatic invasion and lymph node metastasis present in 6 of 10 (60%) animals. Immunohistochemically, 10 of 10 (100%) expressed cytokeratin; 7 of 9 (77%) expressed E-cadherin; and 8 of 9 (88%) expressed beta-catenin. The expression of E-cadherin and beta-catenin was decreased in the cell membrane and increased in the cytoplasm. No Helicobacter-like bacteria were observed via silver stain, and callitrichine herpesvirus 3 was detected by polymerase chain reaction with equal frequency from neoplastic and nonneoplastic intestinal sections. The tumors described in this population illustrate comparable features to human cases of small intestine carcinoma and may serve as a potential animal model for small intestinal carcinomas.
Miller
A D
AD
New England Regional Primate Research Center, Harvard Medical School, One Pine Hill Drive, Southborough, MA 01760, USA. Andrew_Miller@hms.harvard.edu
Kramer
J A
JA
Lin
K C
KC
Knight
H
H
Martinot
A
A
Mansfield
K G
KG
D000230:Adenocarcinoma / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D002144:Callithrix; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D007414:Intestinal Neoplasms / Q000473:pathology / Q000662:veterinary*; D007421:Intestine, Small / Q000473:pathology*; D008207:Lymphatic Metastasis / Q000473:pathology*; D008297:Male; D008992:Monkey Diseases / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012189:Retrospective Studies
D004273:DNA, Neoplasm
Lab Anim Sci. 1990 Mar;40(2):210-2
2157108
Mol Carcinog. 2006 May;45(5):349-52
16493667
Cancer. 2004 Aug 1;101(3):518-26
15274064
Mod Pathol. 2006 Apr;19(4):573-80
16501564
J Exp Clin Cancer Res. 2009 Feb 03;28:14
19192297
Comp Med. 2000 Oct;50(5):540-4
11099139
FEMS Microbiol Lett. 2007 Jun;271(2):239-44
17439542
J Am Vet Med Assoc. 1984 Dec 1;185(11):1377-8
6511587
ANZ J Surg. 2001 Jul;71(7):428-37
11450920
Cancer. 1999 Dec 15;86(12):2693-706
10594865
Infect Immun. 2008 Jun;76(6):2758-66
18411292
J Natl Cancer Inst. 1972 Feb;48(2):325-46
4652379
Am J Primatol. 2005 Jun;66(2):111-8
15940705
J Med Primatol. 1998 Dec;27(6):319-24
10203013
J Virol. 1997 May;71(5):4138-44
9094697
Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):1224-9
11158621
Cell Cycle. 2009 Aug 15;8(16):2549-56
19597346
Gastroenterology. 1996 Jan;110(1):102-15
8536845
J Clin Microbiol. 1999 Jan;37(1):146-51
9854080
Science. 2004 Mar 5;303(5663):1483-7
15001769