Potyviral resistance derived from cultivars of Phaseolus vulgaris carrying bc-3 is associated with the homozygotic presence of a mutated eIF4E allele.
Eukaryotic translation initiation factors (eIFs) play a central role in potyviral infection. Accordingly, mutations in the gene encoding eIF4E have been identified as a source of recessive resistance in several plant species. In common bean, Phaseolus vulgaris, four recessive genes, bc-1, bc-2, bc-3 and bc-u, have been proposed to control resistance to the potyviruses Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus. In order to identify molecular entities for these genes, we cloned and sequenced P. vulgaris homologues of genes encoding the eIF proteins eIF4E, eIF(iso)4E and nCBP. Bean genotypes reported to carry bc-3 resistance were found specifically to carry non-silent mutations at codons 53, 65, 76 and 111 in eIF4E. This set of mutations closely resembled a pattern of eIF4E mutations determining potyvirus resistance in other plant species. The segregation of BCMV resistance and eIF4E genotype was subsequently analysed in an F(2) population derived from the P. vulgaris all-susceptible genotype and a genotype carrying bc-3. F(2) plants homozygous for the eIF4E mutant allele were found to display at least the same level of resistance to BCMV as the parental resistant genotype. At 6 weeks after inoculation, all F(2) plants found to be BCMV negative by enzyme-linked immunosorbent assay were found to be homozygous for the mutant eIF4E allele. In F(3) plants homozygous for the mutated allele, virus resistance was subsequently found to be stably maintained. In conclusion, allelic eIF4E appears to be associated with a major component of potyvirus resistance present in bc-3 genotypes of bean.
Naderpour
Masoud
M
Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark. m.naderpour@dias.kvl.dk
Lund
Ole Søgaard
OS
Larsen
Richard
R
Johansen
Elisabeth
E
D000483:Alleles; D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000276:immunology; D020090:Chromosome Segregation / Q000235:genetics; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D039561:Eukaryotic Initiation Factor-4E / Q000737:chemistry / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D005808:Genes, Recessive / Q000235:genetics; D006720:Homozygote; D007113:Immunity, Innate / Q000276:immunology; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D027805:Phaseolus / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D010935:Plant Diseases / Q000276:immunology* / Q000821:virology; D010940:Plant Proteins / Q000378:metabolism; D011110:Polymorphism, Genetic; D017800:Potyvirus / Q000276:immunology / Q000502:physiology*; D039381:RNA Cap-Binding Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA
D000956:Antigens, Viral; D018076:DNA, Complementary; D039561:Eukaryotic Initiation Factor-4E; D010940:Plant Proteins; D039381:RNA Cap-Binding Proteins
J Virol. 2003 Aug;77(15):8181-6
12857886
Curr Biol. 2002 Jun 25;12(12):1046-51
12123581
Virology. 1997 Jul 21;234(1):84-92
9234949
Plant J. 2002 Dec;32(6):1067-75
12492847
Mol Plant Microbe Interact. 1998 Feb;11(2):124-30
9450335
Mol Cells. 2009 Mar 31;27(3):329-36
19326080
Mol Plant Microbe Interact. 2004 Mar;17(3):322-9
15000399
J Gen Virol. 2006 Jul;87(Pt 7):2089-2098
16760413
Plant Physiol. 2003 Jul;132(3):1272-82
12857809
Plant J. 2005 Jun;42(6):912-22
15941403
Mol Plant Microbe Interact. 2000 Nov;13(11):1266-70
11059494
Trends Plant Sci. 2006 Jan;11(1):40-5
16343979
Phytopathology. 2005 Sep;95(9):1037-42
18943301
Plant J. 2005 May;42(3):392-405
15842624
Plant J. 2004 Nov;40(3):376-85
15469495
J Gen Virol. 2004 Apr;85(Pt 4):1055-1063
15039548
FEBS Lett. 2005 Feb 14;579(5):1167-71
15710407
Biochemistry. 1991 Feb 12;30(6):1624-7
1993179
J Gen Virol. 2007 Nov;88(Pt 11):3177-3186
17947545
J Virol. 2007 Jan;81(2):775-82
17079311
Virology. 2000 Aug 1;273(2):300-6
10915600
Plant Cell. 2007 Sep;19(9):2913-28
17890375
Genome. 1996 Dec;39(6):1216-9
8983191
Mol Plant Microbe Interact. 2007 Sep;20(9):1075-82
17849710
J Biol Chem. 1998 Apr 24;273(17):10325-30
9553087