title: Prime-boost vaccination using chemokine-fused gp120 DNA and HIV envelope peptides activates both immediate and long-term memory cellular responses in rhesus macaques.
abstract: HIV vaccine candidates with improved immunogenicity and induction of mucosal T-cell immunity are needed. A prime-boost strategy using a novel HIV glycoprotein 120 DNA vaccine was employed to immunize rhesus macaques. The DNA vaccine encoded a chimeric gp120 protein in fusion with monocyte chemoattractant protein-3, which was hypothesized to improve the ability of antigen-presenting cells to capture viral antigen through chemokine receptor-mediated endocytosis. DNA vaccination induced virus-reactive T cells in peripheral blood, detectable by T cell proliferation, INFgamma ELISPOT and sustained IL-6 production, without humoral responses. With a peptide-cocktail vaccine containing a set of conserved polypeptides of HIV-1 envelope protein, given by nasogastric administration, primed T-cell immunity was significantly boosted. Surprisingly, long-term and peptide-specific mucosal memory T-cell immunity was detected in both vaccinated macaques after one year. Therefore, data from this investigation offer proof-of-principle for potential effectiveness of the prime-boost strategy with a chemokine-fused gp120 DNA and warrant further testing in the nonhuman primate models for developing as a potential HIV vaccine candidate in humans.
authors.affiliation: Department of Lymphoma and Myeloma, M. D. Anderson Cancer Center, The University of Texas, Houston, TX 77030, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Qin
authors.forename: Pramod N
authors.initials: PN
authors.lastname: Nehete
authors.forename: Hong
authors.initials: H
authors.lastname: He
authors.forename: Bharti
authors.initials: B
authors.lastname: Nehete
authors.forename: Stephanie
authors.initials: S
authors.lastname: Buchl
authors.forename: Soung-Chul
authors.initials: SC
authors.lastname: Cha
authors.forename: Jagannadha K
authors.initials: JK
authors.lastname: Sastry
authors.forename: Larry W
authors.initials: LW
authors.lastname: Kwak
mesh_terms: D000818:Animals; D054410:Chemokine CCL7 / Q000276:immunology*; D004247:DNA; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D007117:Immunization, Secondary / Q000379:methods*; D007156:Immunologic Memory / Q000276:immunology*; D008253:Macaca mulatta / Q000276:immunology*; D010455:Peptides / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014611:Vaccination / Q000379:methods*
chemical_list: D054410:Chemokine CCL7; D015699:HIV Envelope Protein gp120; D010455:Peptides; D011993:Recombinant Fusion Proteins; D004247:DNA
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