Gold nanorods for fluorescence lifetime imaging in biology.
Two-photon luminescence (TPL) from gold nanorods shows considerable potential in biological imaging. We study the imaging of gold nanorods in Madin-Darby canine kidney (MDCK) cells using fluorescence lifetime imaging microscopy (FLIM). FLIM provides images with better contrast and sensitivity than intensity imaging. The characteristic fluorescence lifetime of gold nanorods is found to be less than 100 ps, which can be used to distinguish gold nanorods from other fluorescent labels and endogenous fluorophores in lifetime imaging.
Zhang
Yinan
Y
University of Strathclyde, SUPA - Scottish Universities Physics Alliance, Department of Physics, Centre for Molecular Nanometrology, Photophysics Group, John Anderson Building, 107 Rottenrow, Glasgow, G4 0NG United Kingdom.
Yu
Jun
J
Birch
David J S
DJ
Chen
Yu
Y
D000818:Animals; D002460:Cell Line; D003287:Contrast Media; D004285:Dogs; D006046:Gold; D007089:Image Enhancement / Q000379:methods*; D007668:Kidney / Q000166:cytology*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D043942:Nanotubes / Q000032:analysis*
D003287:Contrast Media; D006046:Gold