Enhanced production of gamma-aminobutyric acid using rice bran extracts by Lactobacillus sakei B2-16.
An efficient and simple fermentation process was developed for the production of gamma-amminobutyric acid (GABA) by Lactobacillus sakei B2-16. When the L. sakei B2-16 was cultivated in the rice bran extracts medium containing 4% sucrose, 1% yeast extract and 12% monosodium glutamate, the maximum GABA concentration reached 660.0 mM with 100% conversion yield, showing the 2.4-fold higher GABA concentration compared to the modified MRS medium without the rice bran extracts. The GABA production was scaled-up from a laboratory scale (5 L) to a pilot (300 L) and a plant scales (5,000 L) to investigate the application possibility of GABA production to industrial fields. The GABA production at the pilot and plant scales was similar to the laboratory scale using rice bran extracts medium which could be effective for the low-cost production of GABA.
Kook
Moo-Chang
MC
Graduate Program in Biomaterial Science and Engineering, Yonsei University, Seoul 120-749, Korea, R&D Division, Biovan Co. Ltd., YERC B120F, Yonsei University, Seoul 120-749, Korea.
Seo
Myung-Ji
MJ
Cheigh
Chan-Ick
CI
Pyun
Yu-Ryang
YR
Cho
Seok-Cheol
SC
Park
Hoon
H
D007218:Industrial Microbiology / Q000379:methods*; D007778:Lactobacillus / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid