title: Uncertainties in the classification of human cationic trypsinogen (PRSS1) variants as hereditary pancreatitis-associated mutations.
abstract: BACKGROUND
  Autosomal dominant hereditary pancreatitis has been conclusively linked with cationic trypsinogen (PRSS1) mutations p.R122H and p.N29I, which can be found in approximately 90% of mutation-positive cases. To date, 35 additional rare or private PRSS1 variants have been identified in subjects with hereditary or sporadic, idiopathic chronic pancreatitis. Despite the lack of sufficient genetic and functional evidence, many of these rare variants have been labelled as pancreatitis associated. This problematic trend is notably illustrated by two recent studies that classified the p.A121T PRSS1 variant as pancreatitis associated, in large part owing to its intimate proximity to arginine-122, the residue affected by the disease causing p.R122H mutation.
  
  
  RESULTS
  Here we demonstrate that the p.A121T variant is functionally innocuous and shows no verifiable association with hereditary pancreatitis, on the basis of the available inconclusive data.
  
  
  CONCLUSIONS
  This case cautions that assignment of clinical relevance to rare PRSS1 variants should not be based on a perceived analogy with genuine disease causing PRSS1 mutations, and further studies are required to prove or rule out possible low penetrance causality of rare PRSS1 variants.
authors.forename: Richárd
authors.initials: R
authors.lastname: Szmola
authors.forename: Miklós
authors.initials: M
authors.lastname: Sahin-Tóth
mesh_terms: D019943:Amino Acid Substitution; D005260:Female; D005799:Genes, Dominant; D014644:Genetic Variation; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009154:Mutation; D050500:Pancreatitis, Chronic / Q000201:enzymology* / Q000235:genetics*; D010375:Pedigree; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014357:Trypsin / Q000235:genetics* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; C489303:PRSS1 protein, human; D014357:Trypsin
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