Electrochemical DNA sensor for Neisseria meningitidis detection.
Meningitis sensor based on nucleic acid probe of Neisseria meningitidis has been fabricated by immobilization of 5'-thiol end labeled single stranded deoxyribonucleic acid probe (ssDNA-SH) onto gold (Au) coated glass electrode. This ssDNA-SH/Au electrode hybridized with the genomic DNA (G-dsDNA/Au) and amplified DNA (PCR-dsDNA/Au) has been characterized using atomic force microscopy (AFM), Fourier transforms infrared spectroscopy (FT-IR) and electrochemical techniques. The ssDNA-SH/Au electrode can specifically detect upto 10-60 ng/microl of G-dsDNA-SH/Au and PCR-dsDNA-SH/Au of meningitis within 60s of hybridization time at 25 degrees C by cyclic voltammetry (CV) using methylene blue (MB) as electro-active DNA hybridization indicator. The values of sensitivities of the G-dsDNA-SH/Au and PCR-dsDNA-SH/Au electrodes have been determined as 0.0115 microA/ng cm(-2) and 0.0056 microA/ng cm(-2), respectively with regression coefficient (R) as 0.999. This DNA bioelectrode is stable for about 4 months when stored at 4 degrees C.
Patel
Manoj K
MK
Institute of Genomics and Integrative Biology, Delhi, India.
Solanki
Pratima R
PR
Kumar
Ashok
A
Khare
Shashi
S
Gupta
Sunil
S
Malhotra
Bansi D
BD
D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics*; D055664:Electrochemical Techniques; D005798:Genes, Bacterial; D006046:Gold; D006801:Humans; D018625:Microscopy, Atomic Force; D009345:Neisseria meningitidis / Q000235:genetics* / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D017550:Spectroscopy, Fourier Transform Infrared
D015342:DNA Probes; D004269:DNA, Bacterial; D006046:Gold