Application of PRP gel alone or in combination with guided bone regeneration does not enhance bone healing process: An experimental study in rabbits.
BACKGROUND
The aim of this study was to assess the hypothesis that application of platelet-rich plasma (PRP) gel in mandibular defects in rabbits, alone or in combination with guided bone regeneration (GBR) techniques, could enhance the bone healing process.


METHODS
Thirty New Zealand white rabbits were used. Three groups of 10 animals each were assigned, and the animals were sacrificed after 12 weeks. During the operation, a rotating trephine bur was used to create circular defects 10-mm in diameter in the region anterior to the jaw angles. In group human fascia lata (HFL), a human fascia lata membrane was used. In group PRP, PRP gel was used to fill the defect, and in group HFL+PRP, PRP was used to fill the defect which after that was covered with a human fascia lata membrane.


RESULTS
In general, none of the control sides and the PRP treated sides had full development of bone or filling of the defect through bone bridging. Conversely, the sides on which the fascia lata membrane or the combination of membrane and PRP had been applied were characterized mostly by development of newly formed bone that bridged the gap.


CONCLUSIONS
Our results suggest that the application of PRP gel alone or in combination with GBR does not enhance bone healing process.
Kazakos
Konstantinos
K
Democritus University of Thrace, Department of Orthopaedics and Traumatology, Dragana, 68100 Alexandroupolis, Greece.
Lyras
Dimitrios Nikolaos
DN
Thomaidis
Vasilios
V
Agrogiannis
George
G
Botaitis
Sotirios
S
Drosos
George
G
Kokka
Anna
A
Verettas
Dionysios
D
D043925:Angiogenesis Inducing Agents / Q000032:analysis / Q000627:therapeutic use; D000818:Animals; D000077214:Becaplermin; D001861:Bone Regeneration / Q000502:physiology*; D003238:Connective Tissue / Q000473:pathology; D004815:Epidermal Growth Factor / Q000032:analysis / Q000627:therapeutic use; D005206:Fascia Lata; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D008297:Male; D008334:Mandible / Q000473:pathology / Q000601:surgery*; D008336:Mandibular Diseases / Q000473:pathology / Q000601:surgery; D008567:Membranes, Artificial; D018482:Muscle, Skeletal / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000627:therapeutic use; D053657:Platelet-Rich Plasma / Q000737:chemistry; D020574:Proto-Oncogene Proteins c-sis; D011817:Rabbits; D053773:Transforming Growth Factor beta1 / Q000032:analysis / Q000627:therapeutic use; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000627:therapeutic use; D014945:Wound Healing / Q000502:physiology
D043925:Angiogenesis Inducing Agents; D008567:Membranes, Artificial; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D000077214:Becaplermin; D004815:Epidermal Growth Factor