Characterization and alternative splicing of the complex I 19-kD subunit in Dunaliella salina: expression and mutual correlation of splice variants under diverse stresses.
Complex I is the first enzyme in the mitochondrial respiratory chain. It extracts energy from NADH, which is produced by the oxidation of sugars and fats, and traps the energy by virtue of a potential difference or voltage across the mitochondrial inner membrane. Herein, the genomic sequence and four splice variants encoding the complex I 19-kD subunit were isolated from Dunaliella salina. There were four transcripts coding for the complex I 19-kD subunit due to alternative splicing in algae, and the four transcripts were translated to two protein isoforms with varying C-terminals. We report the splicing pattern in the 3'-region of the D. salina 19-kD subunit, in which three of the exons (5, 6, and 7) could be alternatively spliced. Moreover, we found that four alternatively spliced variants were subject to coordinated transcription in response to different stresses by real-time quantitative PCR.
Cao
Yu
Y
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chendu, Sichuan, PR China.
Jin
Nan
N
Xu
Hui
H
Liu
Yi
Y
Zhu
Wei Hua
WH
Li
Xin Ran
XR
Qiao
Dai Rong
DR
Cao
Yi
Y
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D042967:Electron Transport Complex I / Q000737:chemistry / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics*; D022821:RNA Splice Sites / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*; D016823:Volvocida / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology
D007527:Isoenzymes; D021122:Protein Subunits; D022821:RNA Splice Sites; D042967:Electron Transport Complex I