[Roles of REV3 in proliferation and genomic stability of colon carcinoma cells].
To investigate the roles of human REV3 gene in proliferation and genomic stability, we experimentally suppressed the REV3 expression in human colon cancer cells (SW480) by the interference RNA technology (RNAi) as monitored by real-time RT-PCR. Compared to untreated or mock-treated cells, ablation of REV3 significantly reduced cell growth rate, micronucleus formation and the frequency of sister chromatid exchange. Whereas the differences between untreated and mock-treated controls were insignificant. Indicators of cell cycle, proliferation and the expression of genetic information in cells of case group, which displayed lower-level expression of REV3, were extremely lower than the control group, and the difference was significant (P<0.05). Differences in the two control groups were not significant. This suggested that the reduced expression of REV3 may affect the growth and proliferation of colon cancer cells (SW480), and, to some extent, contribute to suppression of the genetic instability occurred in micronuclei and sister chromatids. Based on the results from this study, REV3 plays an important role in different cellular growth periods and physiological conditions, and its differential expression directly affects the development of human colon cancer cells.
Sui
Yu
Y
Ningxia Key Laboratory of Reproductive and Genetic, Department of Medical Genetics and Cell Biology, Ningxia Medical College, Yinchuan 750004, China. yu.sui@hotmail.com
Li
Yuan-Jie
YJ
Jin
Cai-Xia
CX
Xu
Fang
F
D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D042822:Genomic Instability; D006801:Humans; D048629:Micronuclei, Chromosome-Defective; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012854:Sister Chromatid Exchange / Q000235:genetics
D004268:DNA-Binding Proteins; D004259:DNA-Directed DNA Polymerase; C118285:REV3L protein, human