Deposition of chemically reactive and repellent sites on biosensor chips for reduced non-specific binding.
The performances of new polymeric materials with excellent optical properties and good machinability have led the biomedical diagnostics industry to develop cheap disposable biosensor platforms appropriate for point of care applications. Zeonor, a type of cycloolefin polymer (COP), is one such polymer that presents an excellent platform for biosensor chips. These polymer substrates have to be modified to have suitable physico-chemical properties for immobilizing proteins. In this work, we have demonstrated the amine functionalization of COP substrates, by plasma enhanced chemical vapour deposition (PECVD), through codeposition of ethylene diamine and 3-aminopropyltriethoxysilane precursors, for building chemistries on the plastic chip. The elemental composition, adhesion, ageing and reactivity of the plasma polymerized film were examined. The Si-O functionality present in amino silane contributed for a good interfacial adhesion of the coating to COP substrates and also acted as a network building layer for plasma polymerization. Wet chemical modification was then carried out on the amine functionalized chips to create chemically reactive isothiocyanate sites and protein repellent fluorinated sites on the same chip. The density of the reactive and repellent sites was altered by choosing appropriate mixtures of homofunctional phenyldiisothiocyanate (PDITC), pentafluoroisothiocyanate (5FITC) and phenylisothiocyanate (PITC) compounds. By tailoring the density of reactive binding sites and protein repellent sites, the non-specific binding of ssDNA has been decreased to a significant extent.
Gandhiraman
R P
RP
Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland. ramprasad.gandhiraman@dcu.ie
Gubala
V
V
Le
N C H
NC
Nam
Le Cao Hoai
le CH
Volcke
C
C
Doyle
C
C
James
B
B
Daniels
S
S
Williams
D E
DE
D000588:Amines / Q000737:chemistry; D001667:Binding, Competitive; D015374:Biosensing Techniques / Q000379:methods*; D003516:Cycloparaffins / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D005029:Ethylenediamines / Q000737:chemistry; D046208:Microchip Analytical Procedures / Q000379:methods*; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry*; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D017155:Wettability
D000588:Amines; D003516:Cycloparaffins; D004277:DNA, Single-Stranded; D005029:Ethylenediamines; D011108:Polymers; D011437:Propylamines; D012821:Silanes; C031234:ethylenediamine; C477625:amino-propyl-triethoxysilane