title: PCR detection of Streptococcus mutans and Aggregatibacter actinomycetemcomitans in dental plaque samples from Haitian adolescents.
abstract: Streptococcus mutans and Aggregatibacter actinomycetemcomitans are oral pathogens associated with dental caries and periodontitis, respectively. The aim of this study was to determine the colonization of these two microorganisms in the dental plaque of a group of Haitian adolescents using two different polymerase chain reaction (PCR) methods, standard PCR, and quantitative real-time PCR (qPCR) assays. Fifty-four pooled supra-gingival plaque samples and 98 pooled sub-gingival plaque samples were obtained from 104 12- to19-year-old rural-dwelling Haitians. The total genomic DNA of bacteria was isolated from these samples, and all participants also received caries and periodontal examinations. Caries prevalence was 42.2%, and the mean decayed, missing, and filled surface (DMFS) was 2.67 ± 5.3. More than half of the adolescents (53.3%) experienced periodontal pockets (Community Periodontal Index score ≥3). S. mutans was detected in 67.3% by qPCR and 38.8% by PCR of the supra-gingival plaque samples (p < 0.01), and 36.6% by qPCR and 8.1% by PCR of the sub-gingival samples (p < 0.01). A. actinomycetemcomitans was detected in 85.1% by qPCR and 44.0% by PCR of the sub-gingival samples (p < 0.01), but the prevalence was similar, 67.3% by qPCR and 59.2% by PCR, in the supra-gingival plaque samples. Neither age nor gender was significantly correlated to the bacterial colonization. The results demonstrated a moderate-to-high prevalence of S. mutans and A. actinomycetemcomitans in the Haitian adolescent population, and qPCR is more sensitive than standard PCR in field conditions. These findings suggest that qPCR should be considered for field oral epidemiologic studies and may be necessary in investigations having major logistic challenges.
authors.affiliation: Department of Epidemiology and Health Promotion, New York University College of Dentistry, 345 E. 24th Street, New York, NY 10010-4086, USA.
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Psoter
authors.forename: Yao
authors.initials: Y
authors.lastname: Ge
authors.forename: Stefanie L
authors.initials: SL
authors.lastname: Russell
authors.forename: Zhou
authors.initials: Z
authors.lastname: Chen
authors.forename: Ralph V
authors.initials: RV
authors.lastname: Katz
authors.forename: Germain
authors.initials: G
authors.lastname: Jean-Charles
authors.forename: Yihong
authors.initials: Y
authors.lastname: Li
mesh_terms: D000293:Adolescent; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification*; D002648:Child; D004245:DMF Index; D004269:DNA, Bacterial / Q000032:analysis; D003731:Dental Caries / Q000145:classification / Q000382:microbiology; D003773:Dental Plaque / Q000382:microbiology*; D003793:Dental Restoration, Permanent / Q000706:statistics & numerical data; D005260:Female; D005888:Gingival Pocket / Q000145:classification / Q000382:microbiology; D006205:Haiti; D006801:Humans; D008297:Male; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012423:Rural Health; D013295:Streptococcus mutans / Q000302:isolation & purification*; D016388:Tooth Loss / Q000145:classification; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial
references.citation: J Clin Periodontol. 2005 Aug;32(8):860-6
references.pmid: 15998269
references.citation: Eur J Oral Sci. 1995 Feb;103(1):36-41
references.pmid: 7600248
references.citation: J Clin Microbiol. 2007 Jan;45(1):81-7
references.pmid: 17079495
references.citation: Acta Odontol Latinoam. 2009;22(1):15-20
references.pmid: 19601491
references.citation: J Clin Periodontol. 2007 Feb;34(2):156-63
references.pmid: 17309590
references.citation: Oral Microbiol Immunol. 2003 Feb;18(1):50-3
references.pmid: 12588459
references.citation: J Clin Periodontol. 2002;29 Suppl 3:10-21; discussion 37-8
references.pmid: 12787203
references.citation: J Clin Microbiol. 2002 Sep;40(9):3334-40
references.pmid: 12202575
references.citation: J Microbiol Methods. 2004 Oct;59(1):117-25
references.pmid: 15325758
references.citation: J Dent Res. 1994 Mar;73(3):672-81
references.pmid: 8163737
references.citation: Community Dent Oral Epidemiol. 2008 Apr;36(2):179-89
references.pmid: 18333882
references.citation: Oral Microbiol Immunol. 1986 Nov;1(1):15-22
references.pmid: 3295677
references.citation: J Clin Microbiol. 2007 Dec;45(12):3859-69
references.pmid: 17942658
references.citation: Clin Microbiol Rev. 2006 Jan;19(1):165-256
references.pmid: 16418529
references.citation: J Clin Periodontol. 2000 Aug;27(8):549-57
references.pmid: 10959780
references.citation: J Clin Periodontol. 1999 Dec;26(12):814-21
references.pmid: 10599910
references.citation: FEMS Microbiol Lett. 2007 Jul;272(2):154-62
references.pmid: 17521362
references.citation: J Periodontal Res. 2003 Oct;38(5):465-70
references.pmid: 12941069
references.citation: J Clin Microbiol. 2000 Mar;38(3):1196-9
references.pmid: 10699021
references.citation: Community Dent Oral Epidemiol. 2009 Jun;37(3):241-9
references.pmid: 19508271
references.citation: J Dent Res. 1980 Oct;59(10):1581-9
references.pmid: 6999044
references.citation: Eur J Oral Sci. 1997 Feb;105(1):9-14
references.pmid: 9085023
references.citation: Infect Immun. 1980 Sep;29(3):1013-20
references.pmid: 6968718
references.citation: J Dent Res. 1999 Apr;78(4):850-6
references.pmid: 10326729
references.citation: FEMS Microbiol Rev. 1995 Dec;17(4):365-79
references.pmid: 8845187
references.citation: J Periodontol. 1983 Dec;54(12):707-11
references.pmid: 6358452
references.citation: J Clin Microbiol. 2000 Jun;38(6):2362-5
references.pmid: 10835003
references.citation: Rev Med Chil. 1989 Aug;117(8):872-8
references.pmid: 2519445
references.citation: Genome Res. 1996 Oct;6(10):986-94
references.pmid: 8908518
references.citation: Arch Oral Biol. 2008 Mar;53(3):231-7
references.pmid: 17983611
references.citation: Microbiol Rev. 1986 Dec;50(4):353-80
references.pmid: 3540569
references.citation: P R Health Sci J. 2008 Mar;27(1):69-74
references.pmid: 18450236
references.citation: J Microbiol Methods. 2000 Jan;39(2):97-107
references.pmid: 10576699
references.citation: Annu Rev Microbiol. 2003;57:29-55
references.pmid: 14527274
references.citation: J Periodontal Res. 2005 Jun;40(3):277-85
references.pmid: 15853975
references.citation: Caries Res. 2006;40(5):366-74
references.pmid: 16946603
references.citation: J Dent Res. 2005 Jun;84(6):559-64
references.pmid: 15914595
references.citation: J Public Health Dent. 2005 Fall;65(4):209-14
references.pmid: 16468462
references.citation: Periodontol 2000. 1999 Jun;20:82-121
references.pmid: 10522224
references.citation: J Clin Periodontol. 2008 Sep;35(8 Suppl):346-61
references.pmid: 18724862
references.citation: BMC Infect Dis. 2006 Jun 13;6:98
references.pmid: 16772039