title: REG gamma: a potential marker in breast cancer and effect on cell cycle and proliferation of breast cancer cell.
abstract: To investigate the expression and significance of proteasomes reactivator REG gamma (γ) in breast cancer. First, we showed the expression of REGγ in breast cancer, metastatic lymph nodes and normal breast tissues. Meanwhile, we also analyzed the relationship between REGγ and estrogen receptor (ER), CerBb-2, lymph nodes metastasis and clinical stage of breast cancer. REGγ expression was determined by immunohistochemical staining and western blot. Secondly, we detected the expression of REGγ and REGγ-mRNA in human breast cancer cell lines (MDA-MB-231, MCF-7) and human breast ductal epithelial cell line (HBL-100) by western blot and real-time PCR. Finally, in order to identify effect of REGγ on breast cancer cell cycle and proliferation, we constructed recombinant plasmid of PcDNA3.1-REGγ and designed siRNA for REGγ in vitro. Cell cycle was assayed by flow cytometer (FCM), proliferation was measured by methyl thiazolyl tetrazolium (MTT). The results demonstrated abnormal high expression of REGγ in breast cancer and its metastatic lymph nodes. REGγ expression was related to breast cancer and its status of ER, CerBb-2 and lymph nodes metastasis. REGγ is one of the potential markers in breast cancer. REGγ could facilitate the growth of breast cancer cells.
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, 400016 Chongqing, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
authors.forename: Shengfen
authors.initials: S
authors.lastname: Tu
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Tan
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Liang
authors.initials: L
authors.lastname: Ran
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Rodier
authors.forename: Guosheng
authors.initials: G
authors.lastname: Ren
mesh_terms: D017209:Apoptosis; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D001940:Breast / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D002453:Cell Cycle; D049109:Cell Proliferation; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D061988:Proteasome Inhibitors; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D001324:Autoantigens; C099558:Ki antigen; D061988:Proteasome Inhibitors; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011960:Receptors, Estrogen; D046988:Proteasome Endopeptidase Complex
references.citation: J Biol Chem. 1999 Dec 31;274(53):38211-5
references.pmid: 10608895
references.citation: Eur J Biochem. 1998 Feb 15;252(1):169-77
references.pmid: 9523727
references.citation: Cell Death Differ. 2001 Mar;8(3):256-64
references.pmid: 11319608
references.citation: Eur J Cell Biol. 1995 Mar;66(3):282-92
references.pmid: 7539747
references.citation: J Clin Oncol. 1987 Jan;5(1):55-61
references.pmid: 3806159
references.citation: Exp Mol Med. 2007 Apr 30;39(2):213-21
references.pmid: 17464183
references.citation: Int J Cancer. 1998 Mar 2;75(5):750-6
references.pmid: 9495244
references.citation: FEBS Lett. 1997 Aug 11;413(1):27-34
references.pmid: 9287111
references.citation: J Surg Res. 2001 Sep;100(1):11-7
references.pmid: 11516199
references.citation: Nature. 1991 Jan 10;349(6305):132-8
references.pmid: 1846030
references.citation: Science. 1995 Apr 28;268(5210):533-9
references.pmid: 7725097
references.citation: Oncol Rep. 2001 Mar-Apr;8(2):425-9
references.pmid: 11182068
references.citation: Biochem J. 2000 Jan 1;345 Pt 1:1-15
references.pmid: 10600633
references.citation: Eur J Cell Biol. 1998 Oct;77(2):151-60
references.pmid: 9840465
references.citation: Mol Cell Biol. 1988 Jun;8(6):2504-12
references.pmid: 3043180
references.citation: Int J Cancer. 2001 Aug 15;93(4):481-8
references.pmid: 11477551
references.citation: Mol Biol Rep. 1999 Apr;26(1-2):45-51
references.pmid: 10363646
references.citation: Annu Rev Biochem. 1996;65:801-47
references.pmid: 8811196
references.citation: Arch Biochem Biophys. 2004 May 15;425(2):158-64
references.pmid: 15111123
references.citation: Biochem Biophys Res Commun. 2006 Aug 4;346(3):707-20
references.pmid: 16793020
references.citation: Nat Rev Cancer. 2001 Dec;1(3):222-31
references.pmid: 11902577
references.citation: Mol Reprod Dev. 2008 Feb;75(2):383-91
references.pmid: 17551976
references.citation: Eur J Surg Oncol. 2006 Mar;32(2):153-7
references.pmid: 16360296
references.citation: Cancer. 1993 Jul 1;72(1):120-5
references.pmid: 8099532
references.citation: J Biol Chem. 2001 Jan 12;276(2):1383-90
references.pmid: 11027688
references.citation: Mol Cell. 2007 Jun 22;26(6):843-52
references.pmid: 17588519
references.citation: Int J Biol Markers. 1999 Jan-Mar;14(1):16-26
references.pmid: 10367245
references.citation: Cancer Res. 1991 Dec 15;51(24):6677-85
references.pmid: 1660345
references.citation: J Clin Endocrinol Metab. 2003 Mar;88(3):1374-83
references.pmid: 12629132
references.citation: J Biol Chem. 1998 Sep 25;273(39):25015-9
references.pmid: 9737957
references.citation: J Biol Chem. 1996 Jul 26;271(30):18237-42
references.pmid: 8663520
references.citation: Proc Natl Acad Sci U S A. 1990 Sep;87(18):7071-5
references.pmid: 2205851