Mg2+ substituted calcium phosphate nano particles synthesis for non viral gene delivery application.
Gene therapy provides a unique approach to medicine as it can be adapted towards the treatment of both inherited and acquired diseases. Recently, calcium phosphate vectors as a new generation of the non viral gene delivery nano carriers have been studied because of their biocompatibility and DNA condensation and gene transfer ability. Substituting cations, like magnesium, affects physical and chemical properties of calcium phosphate nano particles. In this study, Mg(2+) substituted calcium phosphate nano particles have been prepared using the simple sol gel method. X-ray diffraction analysis, Fourier transform infra red spectroscopy, transmission electron microscopy, specific surface area analysis, zeta potential measurement and ion release evaluation were used for characterization of the samples. It was concluded that presence of Mg ions decrease particle size and crystallinity of the samples and increase positive surface charge as well as beta tricalcium phosphate fraction in chemical composition of calcium phosphate. These properties result in increasing the DNA condensation ability, specific surface area and dissolution rate of the samples which make them suitable particles for gene delivery application.
Hanifi
A
A
Materials Engineering Department, Isfahan University of Technology, Isfahan, Iran. a.hanifi@gmail.com
Fathi
M H
MH
Sadeghi
H Mir Mohammad
HM
Varshosaz
J
J
D001826:Body Fluids / Q000737:chemistry / Q000502:physiology; D002130:Calcium Phosphates / Q000138:chemical synthesis* / Q000737:chemistry; D003460:Crystallization; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D018014:Gene Transfer Techniques; D008274:Magnesium / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
D002130:Calcium Phosphates; D004337:Drug Carriers; C020243:calcium phosphate; D008274:Magnesium
Expert Rev Mol Diagn. 2005 Nov;5(6):893-905
16255631
Acta Biomater. 2009 Oct;5(8):3141-9
19446055
Acta Biomater. 2009 May;5(4):1233-40
19121609
Soil Sci Soc Am J. 1999 May-Jun;63(3):657-64
11543409
J Inorg Biochem. 1997 Apr;66(1):1-6
9076968
Curr Opin Biotechnol. 2002 Apr;13(2):128-31
11950563
J Osaka Univ Dent Sch. 1986 Dec;26:79-89
3470491
AAPS J. 2007 Mar 23;9(1):E92-104
17408239
Biomaterials. 2004 Aug;25(19):4647-57
15120511
Biomaterials. 2005 Jun;26(17):3379-84
15621226
J Mater Sci Mater Med. 2005 May;16(5):455-60
15875256
J Inorg Biochem. 2009 Dec;103(12):1666-74
19819556
Biomaterials. 2007 Feb;28(6):1267-79
17123600
Biomaterials. 2006 Jun;27(16):3147-53
16469375
Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7297-301
7638184
Acta Biomater. 2007 Sep;3(5):785-93
17512809