Photosynthetic responses of lettuce to downy mildew infection and cytokinin treatment.
Changes in primary metabolism of lettuce, Lactuca sativa L. (cv. Cobham Green), induced by compatible interaction with the biotrophic oomycete pathogen Bremia lactucae Regel (race BL 16), under two intensities of illumination in the presence and absence of exogenous cytokinins were studied by chlorophyll fluorescence imaging. Thirteen days post-inoculation leaf discs infected by B. lactucae exhibited impairments of photosynthesis associated with biotrophic infections, including: reductions in photosynthetic pigment contents and the maximum quantum yield of photosystem II photochemistry (F(V)/F(M)), inhibition of electron transport (Phi(PSII)) and increased non-photochemical chlorophyll fluorescence quenching (NPQ). Detected changes in photosynthetic parameters correlated with the leaf area colonized by the pathogen's intercellular hyphae. Applications of two cytokinins, benzylaminopurine and meta-topolin, previously shown to suppress B. lactucae sporulation if applied 24 h prior to inoculation at a concentration of 200 microM, retarded the pathogen's asexual reproduction with no apparent negative effects on the host's photosynthetic apparatus. However, long-lasting treatment of healthy tissues with this high concentration of exogenous cytokinin led to effects parallel to pathogenesis: reductions in photosynthetic pigment contents accompanied by inhibition of photosystem II photochemistry and electron transport. These effects of both prolonged exposure to cytokinins and the pathogenesis were weaker in discs exposed to the lower photosynthetic photon flux density. The role of cytokinins in plant-biotrophic pathogen interactions and their potential as disease control agents are discussed.
Prokopová
Jitka
J
Department of Experimental Physics, Faculty of Science, Palacký University, Tr. 17. listopadu 12, 771 46 Olomouc, Czech Republic. prokopova.jita@gmail.com
Spundová
Martina
M
Sedlárová
Michaela
M
Husicková
Alexandra
A
Novotný
Radko
R
Dolezal
Karel
K
Naus
Jan
J
Lebeda
Ales
A
D001593:Benzyl Compounds / Q000494:pharmacology; D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism / Q000648:ultrastructure; D003583:Cytokinins / Q000494:pharmacology*; D005453:Fluorescence; D054884:Host-Pathogen Interactions; D018545:Lactuca / Q000378:metabolism / Q000382:microbiology* / Q000502:physiology; D046529:Microscopy, Electron, Transmission; D009868:Oomycetes / Q000502:physiology*; D010788:Photosynthesis / Q000187:drug effects*; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology / Q000502:physiology; D011687:Purines / Q000494:pharmacology
D001593:Benzyl Compounds; D003583:Cytokinins; D045332:Photosystem II Protein Complex; D010937:Plant Growth Regulators; D011687:Purines; D002734:Chlorophyll; C480551:benzylaminopurine