title: Molecular detection of genotype 3 porcine hepatitis E virus in aborted fetuses and their sows.
abstract: By reverse transcription (RT)-nested polymerase chain reaction (PCR), hepatitis E virus (HEV) RNA was simultaneously detected in the livers of aborted fetuses and in fecal and serum samples from their sows, obtained from two of 14 farms tested. Sequence and phylogenetic analyses showed that these HEVs belonged to genotype 3 HEV. HEV RNA was detected by real-time RT-PCR at 2.0 x 10(3)-2.8 x 10(4) viral copies/microl in the HEV-positive samples. All HEV-positive fetuses tested positive for porcine circovirus type 2 (PCV2). These results indicate that transplacental infection of HEV with PCV2 coinfection may occur in sows with reproductive failure, which is suggestive of similarities to HEV infection in women.
authors.affiliation: Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Myra
authors.initials: M
authors.lastname: Hosmillo
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Jeong
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jun-Gyu
authors.initials: JG
authors.lastname: Park
authors.forename: Mukti Kant
authors.initials: MK
authors.lastname: Nayak
authors.forename: Mia Madel
authors.initials: MM
authors.lastname: Alfajaro
authors.forename: Therese Marie
authors.initials: TM
authors.lastname: Collantes
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ikuta
authors.forename: Mikihiro
authors.initials: M
authors.lastname: Yunoki
authors.forename: Mun-Il
authors.initials: MI
authors.lastname: Kang
authors.forename: Sang-Ik
authors.initials: SI
authors.lastname: Park
authors.forename: Kyoung-Oh
authors.initials: KO
authors.lastname: Cho
mesh_terms: D037881:Aborted Fetus / Q000821:virology*; D000034:Abortion, Veterinary / Q000821:virology; D000818:Animals; D005260:Female; D005838:Genotype; D016751:Hepatitis E / Q000662:veterinary* / Q000821:virology; D016752:Hepatitis E virus / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000662:veterinary* / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000821:virology*