Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain.
A technique developed to determine naphthalene dioxygenase (NDO) activity was optimized and used to study the biotransformation of indole to indigo by Pseudomonas sp. J26 whole cells. The maximum production of indigo was achieved at 25 degrees C using 2.5 mM indole when J26 was grown in the complex medium JPP, while indole concentrations higher than 4 mM proved toxic for cells. The maximum rate of indigo production was 0.56 nmol min(-1) mg dry biomass(-1), obtaining 75.5 microM of indigo after 8 h of incubation, while a maximal concentration (138.1 microM) of indigo was obtained after 20 h.
Mercadal
Juan Pablo Riva
JP
Planta Piloto de Procesos Industriales Microbiológicos PROIMI-CONICET Av Belgrano y Caseros T4001MVB Tucumán, Argentina.
Isaac
Paula
P
Siñeriz
Faustino
F
Ferrero
Marcela Alejandra
MA
D001118:Argentina; D003470:Culture Media / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D007203:Indigo Carmine; D007211:Indoles / Q000378:metabolism*; D009281:Naphthalenes / Q000378:metabolism*; D011549:Pseudomonas / Q000302:isolation & purification* / Q000378:metabolism*; D013696:Temperature
D003470:Culture Media; D007211:Indoles; D009281:Naphthalenes; C031721:naphthalene; C030374:indole; D007203:Indigo Carmine