Cloning, purification, crystallization and X-ray crystallographic analysis of Ignicoccus hospitalis neelaredoxin.
Superoxide reductases (SORs) are metalloproteins which constitute the most recently identified oxygen-detoxification system in anaerobic and microaerobic bacteria and archaea. SORs are involved in scavenging superoxide radicals from the cell by catalyzing the reduction of superoxide ({\rm O}_{2};{\bullet -}) to hydrogen peroxide and are characterized by a catalytic nonhaem iron centre coordinated by four histidine ligands and one cysteine ligand. Ignicoccus hospitalis, a hyperthermophilic crenarchaeon, is known to have a neelaredoxin-type SOR that keeps toxic oxygen species levels under control. Blue crystals of recombinant I. hospitalis oxidized neelaredoxin (14.1 kDa, 124 residues) were obtained. These crystals diffracted to 2.4 A resolution in-house at room temperature and belonged to the hexagonal space group P6(2)22 or P6(4)22, with unit-cell parameters a = b = 108, c = 64 A. Cell-content analysis indicated the presence of one monomer in the asymmetric unit.
Pinho
Filipa G
FG
Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2701-901 Oeiras, Portugal.
Romão
Célia V
CV
Pinto
Ana F
AF
Saraiva
Lígia M
LM
Huber
Harald
H
Matias
Pedro M
PM
Teixeira
Miguel
M
Bandeiras
Tiago M
TM
D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D019620:Desulfurococcaceae / Q000201:enzymology*; D033862:Iron-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D033862:Iron-Binding Proteins; D013482:Superoxide Dismutase
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