title: Human papillomavirus genotype in cervical intraepithelial neoplasia grades 2 and 3 of Taiwanese women.
abstract: We aimed to assess the distribution of human papillomavirus (HPV) genotypes in high-grade cervical lesions in Taiwan. The study included 1,086 paraffin-embedded, formaldehyde-fixed cervical intraepithelial neoplasia (CIN) 2/3 specimens. HPV genotyping was performed using polymerase chain reaction (PCR)-based methods. Multiple HPV types were validated by E6 type-specific PCR, direct sequencing and/or real-time PCR. HPV DNA was detected in 995 (91.6%) specimens, and multiple HPV types were identified in 192 (19.3%) samples. The leading HPV types were HPV16 (24%), HPV52 (20%), HPV58 (20%), HPV33 (13%), HPV31 (8%) and HPV18 (4.6%). Although the leading six types consisted of 87.6%, HPV16 or 18 comprised only 30.9%. The prevalence of different HPV types showed a significant association with age. In women older than 50 yr, HPV16 and 18 comprised 21.3% (83/389), while HPV52, 58 and 33 represented 55.5% (216/389). In women aged less than 50 yr, HPV16 and 18 comprised 32.1% (224/697, p < 0.0001), while HPV 52, 58 and 33 represented 47.9% (334/697, p = 0.02). The distribution of HPV genotypes was compared with previously reported findings for Taiwanese women with cervical cancer (CC). The overall HPV16 positivity rate was significantly higher in CC than in CIN 2/3 (odds ratio: 2.14, 95% CI: 1.91-2.40). In addition, HPV18, 39 and 45 were significantly overrepresented in CC, whereas HPV52, 58, 33, 31, 35, 51 and 53 were underrepresented. We concluded that an effective vaccine against the most common HPV types could prevent a significant proportion of cervical cancer cases that occur in Taiwan.
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan.
authors.forename: Angel
authors.initials: A
authors.lastname: Chao
authors.forename: Mei-Shan
authors.initials: MS
authors.lastname: Jao
authors.forename: Chu-Chun
authors.initials: CC
authors.lastname: Huang
authors.forename: Huei-Jean
authors.initials: HJ
authors.lastname: Huang
authors.forename: Hui-Hsin
authors.initials: HH
authors.lastname: Cheng
authors.forename: Jung-Erh
authors.initials: JE
authors.lastname: Yang
authors.forename: Swei
authors.initials: S
authors.lastname: Hsueh
authors.forename: Tse-Ching
authors.initials: TC
authors.lastname: Chen
authors.forename: Jian-Tai
authors.initials: JT
authors.lastname: Qiu
authors.forename: Cheng-Tao
authors.initials: CT
authors.lastname: Lin
authors.forename: Chang-Jui
authors.initials: CJ
authors.lastname: Fu
authors.forename: Hung-Hsueh
authors.initials: HH
authors.lastname: Chou
authors.forename: Chyong-Huey
authors.initials: CH
authors.lastname: Lai
mesh_terms: D000328:Adult; D000375:Aging; D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005784:Gene Amplification; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D013624:Taiwan; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000473:pathology / Q000821:virology*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral