Correlation of DNA ploidy with progression of cervical cancer.
The majority of squamous cell carcinomas of cervix are preceded by visible changes in the cervix, most often detected by cervical smear. As cervical cancer is preceded by long precancerous stages, identification of the high-risk population through detection of DNA ploidy may be of importance in effective management of this disease. Here we attempted to correlate aneuploid DNA patterns and their influence on biological behavior of flow-cytometry analysis of DNA ploidy which was carried out in cytologically diagnosed cases of mild (79), moderate (36), and severe (12) dysplasia, as well as "atypical squamous cells of unknown significance (ASCUS)" (57) along with controls (69), in order to understand its importance in malignant progression of disease. Cytologically diagnosed dysplasias, which were employed for DNA ploidy studies, 39 mild, 28 moderate, and 11 severe dysplasia cases were found to be aneuploid. Out of the 69 control subjects, 6 cases showed aneuploidy pattern and the rest 63 subjects were diploid. An aneuploidy pattern was observed in 8 out of 57 cases of cytologically evaluated ASCUS. The results of the followup studies showed that aberrant DNA content reliably predicts the occurrence of squamous cell carcinoma in cervical smear. Flow cytometric analysis of DNA ploidy may provide a strategic diagnostic tool for early detection of carcinoma cervix. Therefore, it is a concept of an HPV screening with reflex cytology in combination with DNA flow cytometry to detect progressive lesions with the greatest possible sensitivity and specificity.
Singh
M
M
Industrial Toxicology Research Center, P.O. Box 80, M.G. Marg, Lucknow 226001, India.
Mehrotra
S
S
Kalra
N
N
Singh
U
U
Shukla
Y
Y
Cell Oncol. 2004;26(3):143-50
15371650
Clin J Oncol Nurs. 2006 Aug;10(4):527-31
16927906
Eur J Gynaecol Oncol. 2003;24(1):13-7
12691309
Contemp Clin Trials. 2006 Oct;27(5):449-71
16765101
N Engl J Med. 2001 Apr 26;344(17):1270-8
11320386
Anal Quant Cytol Histol. 2002 Feb;24(1):23-9
11865946
Diagn Cytopathol. 2006 Aug;34(8):528-41
16850481
Anal Cell Pathol. 1998;17(4):201-8
10391372
Cancer. 2004 Jun 25;102(3):192-9
15211479
Anal Cell Pathol. 2001;23(2):89-95
11904464
Cancer. 2004 Feb 25;102(1):41-54
14968417
Clin Cancer Res. 2004 May 1;10(9):3059-63
15131043
Cancer. 2001 Jun 25;93(3):222-8
11391611
Am J Obstet Gynecol. 1999 Sep;181(3):560-6
10486464
JAMA. 2000 Jan 5;283(1):87-93
10632285
J Immunol Methods. 1991 Jun 3;139(2):271-9
1710634
Acta Cytol. 2001 Jul-Aug;45(4):499-501
11480708
Cancer Detect Prev. 1997;21(3):242-6
9167041
Curr Opin Oncol. 2000 Jan;12(1):82-8
10687734
Neoplasma. 1990;37(5):497-500
2234211
JAMA. 2000 May 17;283(19):2525-6
10815111
J Natl Cancer Inst. 2001 Jan 17;93(2):92-4
11208877
Int J Gynecol Pathol. 1993 Jul;12(3):193-207
7688352
Cell Cycle. 2004 Jun;3(6):823-8
15197343
Int J Cancer. 1993 Jun 19;54(4):594-606
8514451
J Clin Pathol. 1993 Aug;46(8):700-2
8408692
Virology. 1991 Sep;184(1):9-13
1651607
Cytometry. 2001 Jun 15;46(3):166-71
11449407
Methods Cell Biol. 1994;41:263-96
7861967
Biochim Biophys Acta. 1996 Oct 9;1288(2):F55-78
8876633
Diagn Cytopathol. 2001 Mar;24(3):153-6
11241896
Anal Cell Pathol. 1995 Jan;8(1):67-74
7734413
Cancer. 2003 Apr 25;99(2):113-7
12704691
Pathologe. 1999 Jan;20(1):25-33
10091229
JAMA. 2002 Apr 24;287(16):2114-9
11966386
Anal Quant Cytol Histol. 1990 Oct;12(5):306-13
2176502
J Clin Microbiol. 2003 Feb;41(2):608-12
12574254
Cancer Lett. 1998 Jan 16;123(1):47-52
9461017