[PHOSPHAN microplate technology-based microarray for the determination of IgG antibodies against West Nile and Crimean-Congo hemorrhagic fever viruses].
The paper demonstrates it possible to work out a phosphorescence analysis (PHOSPHAN) microplate technology-based microarray for concurrently examining human sera and detection of their specific IgG antibodies against two heterological West Nile and Crimean-Congo hemorrhagic fever viruses. The sensitivity and specificity of the microarray were comparable with those of enzyme immunoassay with separate sample testing. The advantages of PHOSPHAN were associated with the microplate format of an immunoassay and its enhanced multiplexity, which may contribute to the lower cost of clinical sample testing.
Pomelova
V G
VG
Bychenkova
T A
TA
Laricheva
S Iu
SIu
Osin
N S
NS
Butenko
A M
AM
Larichev
V F
VF
Khutoretskaia
N V
NV
D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D000918:Antibody Specificity; D005455:Fluorescent Antibody Technique; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000276:immunology / Q000302:isolation & purification*; D006479:Hemorrhagic Fever, Crimean / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D014901:West Nile Fever / Q000097:blood / Q000175:diagnosis*; D014902:West Nile virus / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral; D007074:Immunoglobulin G