A highly selective fluorescent probe for detection of biological samples thiol and its application in living cells.
Probe 1 was designed and synthesized as a new fluorescent molecular probe for thiols in PBS buffer at physiological condition. This fluorescent molecular probe consists of a thiol reaction moiety bound to a coumarin fluorophore. Its fluorescence quantum yield is low, but a drastic enhancement of fluorescence intensity was observed in the presence of thiols. Possible interference with other analytes was examined. Probe 1 displays a highly selective fluorescent enhancement with thiols, and the probe was successfully applied to thiols determination in intracellular, in human urine and blood samples.
Zuo
Qing-Ping
QP
Pharmaceutics Department, College of pharmacy, Central South University, Changsha, Hunan, 410013, People's Republic of China.
Li
Bing
B
Pei
Qi
Q
Li
Zuojun
Z
Liu
Shi-Kun
SK
D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D011789:Quantum Theory; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000032:analysis*; D014062:Tongue Neoplasms / Q000737:chemistry* / Q000473:pathology
D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds
Angew Chem Int Ed Engl. 2009;48(22):4034-7
19388016
Anal Chim Acta. 2008 Oct 10;627(2):254-7
18809081
Chemistry. 2009;15(20):5096-103
19343759
J Pharm Biomed Anal. 2008 Dec 15;48(5):1375-80
18926658
Chem Commun (Camb). 2005 Jul 7;(25):3189-91
15968367
Chem Commun (Camb). 2003 Jan 7;(1):146-7
12611008
J Chromatogr. 1985 Feb 27;338(1):209-12
4019645
Anal Chem. 2001 Dec 15;73(24):5972-8
11791568
Bioelectrochemistry. 2004 Jun;63(1-2):19-24
15110242
Anal Chem. 2002 Mar 15;74(6):1349-54
11922303
Amino Acids. 2009 Jul;37(2):395-400
18695935
N Engl J Med. 1997 Jul 24;337(4):230-6
9227928
Bioorg Med Chem Lett. 2008 Apr 1;18(7):2246-9
18358719
Chem Commun (Camb). 2009 Aug 14;(30):4530-2
19617973
Org Lett. 2007 Aug 16;9(17):3375-7
17645349
Angew Chem Int Ed Engl. 2005 May 6;44(19):2922-5
15818626
Anal Biochem. 2007 Dec 1;371(1):71-81
17727809
Anal Chem. 2007 Nov 15;79(22):8769-73
17924649
Chem Commun (Camb). 2004 Aug 7;(15):1762-3
15278174
J Nutr. 2006 Jun;136(6 Suppl):1682S-1693S
16702340
Anal Chim Acta. 2008 Jul 14;620(1-2):120-6
18558132
Org Lett. 2008 Jan 3;10(1):37-40
18062694
Talanta. 2003 Aug 29;60(6):1229-38
18969150
Clin Chem. 1990 Dec;36(12):2137-8
2253363
N Engl J Med. 2002 Feb 14;346(7):476-83
11844848
Clin Chem. 1998 Aug;44(8 Pt 2):1833-43
9702993
J Am Chem Soc. 2003 Jul 2;125(26):7802-3
12822989
Org Lett. 2009 Nov 5;11(21):4918-21
19788282
J Fluoresc. 2009 Jul;19(4):593-9
19050997
Neurosci Lett. 1990 Mar 2;110(1-2):216-20
2325885
Cancer Lett. 2004 May 28;208(2):143-53
15142672
J Am Chem Soc. 2007 Aug 29;129(34):10322-3
17672463
J Clin Invest. 1960 Nov;39(11):1633-8
16695834
Anal Chem. 2000 Dec 1;72(23):5755-60
11128932
Eur J Biochem. 2000 Aug;267(16):4904-11
10931172
Angew Chem Int Ed Engl. 2008;47(41):7883-7
18781571
Spectrochim Acta A Mol Biomol Spectrosc. 2001 Oct;57(12):2403-7
11767834