title: Development and evaluation of the quick anaero-system-a new disposable anaerobic culture system.
abstract: BACKGROUND
  We developed a new disposable anaerobic culture system, namely, the Quick anaero-system, for easy culturing of obligate anaerobes.
  
  
  METHODS
  Our system consists of 3 components: 1) new disposable anaerobic gas pack, 2) disposable culture-envelope and sealer, and 3) reusable stainless plate rack with mesh containing 10 g of palladium catalyst pellets. To evaluate the efficiency of our system, we used 12 anaerobic bacteria. We prepared 2 sets of ten-fold serial dilutions of the 12 anaerobes, and inoculated these samples on Luria-Bertani (LB) broth and LB blood agar plate (LB-BAP) (BD Diagnostic Systems, USA). Each set was incubated in the Quick anaero-system (DAS Tech, Korea) and BBL GasPak jar with BD GasPak EZ Anaerobe Container System (BD Diagnostic Systems) at 35-37 degrees C for 48 hr. The minimal inoculum size showing visible growth of 12 anaerobes when incubated in both the systems was compared.
  
  
  RESULTS
  The minimal inoculum size showing visible growth for 2 out of the 12 anaerobes in the LB broth and 9 out of the 12 anaerobes on LB-BAP was lower for the Quick anaero-system than in the BD GasPak EZ Anaerobe Container System. The mean time (+/-SD) required to achieve absolute anaerobic conditions of the Quick anaero-system was 17 min and 56 sec (+/-3 min and 25 sec).
  
  
  CONCLUSIONS
  The Quick anaero-system is a simple and effective method of culturing obligate anaerobes, and its performance is superior to that of the BD GasPak EZ Anaerobe Container System.
authors.affiliation: Department of Microbiology, Chosun University Medical School, Gwangju, Korea.
authors.forename: Nam Woong
authors.initials: NW
authors.lastname: Yang
authors.forename: Jin Man
authors.initials: JM
authors.lastname: Kim
authors.forename: Gwang Ju
authors.initials: GJ
authors.lastname: Choi
authors.forename: Sook Jin
authors.initials: SJ
authors.lastname: Jang
mesh_terms: D001421:Bacteria, Anaerobic / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D005740:Gases / Q000737:chemistry; D010165:Palladium / Q000737:chemistry; D011933:Reagent Kits, Diagnostic
chemical_list: D003470:Culture Media; D005740:Gases; D011933:Reagent Kits, Diagnostic; D010165:Palladium