Tree-shaped paper strip for semiquantitative colorimetric detection of protein with self-calibration.
This paper described a convenient semiquantitative method for colorimetric detection of protein with self-calibration integrated on the test strip. Hydrophilic paper was employed as microfluidic device for running colorimetric assay, tree-shaped design was developed to ensure uniform microfluidic flow for multiple branches. The approach was validated with bovine serum albumin (BSA) colorimetric detection, and colorimetric results observed by naked eyes were consistent with that from apparatus. The device could be coupled with digital transmission of images for remote monitoring system for diagnosis, food control, and environmental analysis.
Wang
Wei
W
Key Laboratory of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093, China. wangw@ycit.edu.cn
Wu
Wen-Ya
WY
Wang
Wei
W
Zhu
Jun-Jie
JJ
D000818:Animals; D002138:Calibration; D002417:Cattle; D002854:Chromatography, Paper / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000032:analysis
D011506:Proteins; D012710:Serum Albumin, Bovine