Overexpression of AtDOF4.7, an Arabidopsis DOF family transcription factor, induces floral organ abscission deficiency in Arabidopsis.
After flower pollination, a programmed process called abscission occurs in which unwanted floral organs are actively shed from the main plant body. We found that a member of the DOF (for DNA binding with one finger) transcription factor family, Arabidopsis (Arabidopsis thaliana) DOF4.7, was expressed robustly in the abscission zone. The Arabidopsis 35S::AtDOF4.7 lines with constitutive expression of AtDOF4.7 exhibited an ethylene-independent floral organ abscission deficiency. In these lines, anatomical analyses showed that the formation of the abscission zone was normal. However, dissolution of the middle lamella failed to separate between the cell walls. AtDOF4.7 was identified as a nucleus-localized transcription factor. This protein had both in vitro and in vivo binding activity to typical DOF cis-elements in the promoter of an abscission-related polygalacturonase (PG) gene, PGAZAT. Overexpression of AtDOF4.7 resulted in down-regulation of PGAZAT. AtDOF4.7 interacted with another abscission-related transcription factor, Arabidopsis ZINC FINGER PROTEIN2. Taken together, our results suggest that AtDOF4.7 participates in the control of abscission as part of the transcription complex that directly regulates the expression of cell wall hydrolysis enzymes.
Wei
Peng-Cheng
PC
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, People's Republic of China.
Tan
Feng
F
Gao
Xin-Qi
XQ
Zhang
Xiu-Qing
XQ
Wang
Gao-Qi
GQ
Xu
Heng
H
Li
Li-Juan
LJ
Chen
Jia
J
Wang
Xue-Chen
XC
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D005030:Ethylenes / Q000494:pharmacology; D035264:Flowers / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D010641:Phenotype; D030821:Plants, Genetically Modified; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015003:Yeasts / Q000187:drug effects / Q000378:metabolism
D029681:Arabidopsis Proteins; D005030:Ethylenes; D014157:Transcription Factors; C530165:zinc finger protein 2, Arabidopsis; C036216:ethylene
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