Regenerative effect of basic fibroblast growth factor on periodontal healing in two-wall intrabony defects in dogs.
OBJECTIVE
The aim of the present study was to evaluate the effect of a basic fibroblast growth factor (bFGF) candidate treatment on periodontal healing in two-wall intrabony defects in dogs.


METHODS
Two-wall intrabony defects (5 x 5 x 5 mm) were created surgically on the distal and mesial sides of bilateral mandibular second and fourth premolars in four Beagle dogs. bFGF, enamel matrix derivative (EMD) and platelet-derived growth factor with beta-tricalcium phosphate (PDGF/beta-TCP) treatments, and sham-surgery (OFD) were rotated among the four defects in each animal, EMD and PDGF/beta-TCP serving as benchmark controls. The animals were euthanized for radiographic and histologic evaluation at 8 weeks.


RESULTS
Bone formation was significantly greater in the bFGF group (4.11 +/- 0.77 mm) than in the EMD (3.32 +/- 0.71 mm; p<0.05) and OFD (3.09 +/- 0.52 mm; p<0.01) groups. The EMD (4.59 +/- 1.19 mm) and PDGF/beta-TCP (4.66 +/- 0.7 mm) groups exhibited significantly greater cementum regeneration with periodontal ligament-like tissue than the OFD group (2.96 +/- 0.69 mm; p<0.01). No significant differences were observed between the bFGF and the PDGF/beta-TCP groups in any of the histometric parameters.


CONCLUSIONS
The candidate bFGF treatment supported periodontal regeneration comparable with that of established benchmarks: EMD and PDGF/beta-TCP.
Shirakata
Yoshinori
Y
Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan. syoshi@dent.kagoshima-u.ac.jp
Taniyama
Katsuyoshi
K
Yoshimoto
Takehiko
T
Miyamoto
Motoharu
M
Takeuchi
Naoshi
N
Matsuyama
Takashi
T
Noguchi
Kazuyuki
K
D016301:Alveolar Bone Loss / Q000188:drug therapy*; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D018786:Bone Substitutes / Q000627:therapeutic use; D003746:Dental Enamel Proteins / Q000627:therapeutic use; D004195:Disease Models, Animal; D004285:Dogs; D016222:Fibroblast Growth Factor 2 / Q000502:physiology* / Q000627:therapeutic use; D008297:Male; D008334:Mandible / Q000187:drug effects* / Q000601:surgery; D010519:Periodontium / Q000187:drug effects* / Q000502:physiology; D018709:Statistics, Nonparametric
D018786:Bone Substitutes; D003746:Dental Enamel Proteins; C109069:enamel matrix proteins; D016222:Fibroblast Growth Factor 2