title: Overexpression of the recA gene decreases oral but not intraperitoneal fitness of Salmonella enterica.
abstract: Transcription of the Salmonella enterica recA gene is negatively controlled by the LexA protein, the repressor of the SOS response. The introduction of a mutation (recAo6869) in the LexA binding site, in the promoter region of the S. enterica ATCC 14028 recA gene, allowed the analysis of the effect that RecA protein overproduction has on the fitness of this virulent strain. The fitness of orally but not intraperitoneally inoculated recAo6869 cells decreased dramatically. However, the SOS response of this mutant was induced normally, and there was no increase in the sensitivity of the strain toward DNA-damaging agents, bile salts, or alterations in pH. Nevertheless, S. enterica recAo6869 cells were unable to swarm and their capacity to cross the intestinal epithelium was significantly reduced. The swarming deficiency in recAo6869 cells is independent of the flagellar phase. Moreover, swimming activity of the recAo6869 strain was not diminished with respect to the wild type, indicating that the flagellar synthesis is not affected by RecA protein overproduction. In contrast, swarming was recovered in a recAo6869 derivative that overproduced CheW, a protein known to be essential for this function. These data demonstrate that an equilibrium between the intracellular concentrations of RecA and CheW is necessary for swarming in S. enterica. Our results are the first to point out that the SOS response plays a critical role in the prevention of DNA damage by abolishing bacterial swarming in the presence of a genotoxic compound.
authors.affiliation: Departament de Genètica i Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Medina-Ruiz
authors.forename: Susana
authors.initials: S
authors.lastname: Campoy
authors.forename: Cristina
authors.initials: C
authors.lastname: Latasa
authors.forename: Paula
authors.initials: P
authors.lastname: Cardenas
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Alonso
authors.forename: Jordi
authors.initials: J
authors.lastname: Barbé
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018938:Caco-2 Cells / Q000382:microbiology; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007082:Ileum / Q000382:microbiology; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D011938:Rec A Recombinases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012480:Salmonella Infections / Q000382:microbiology*; D019779:Salmonella enterica / Q000235:genetics / Q000472:pathogenicity*
chemical_list: D011938:Rec A Recombinases
references.citation: Vet Microbiol. 2001 May 3;80(1):53-61
references.pmid: 11278123
references.citation: Bioinformatics. 2003 Nov 22;19(17):2225-36
references.pmid: 14630651
references.citation: Microb Pathog. 1999 Jul;27(1):13-23
references.pmid: 10371706
references.citation: J Bacteriol. 1988 Apr;170(4):1467-74
references.pmid: 3127374
references.citation: Bioessays. 2006 Jan;28(1):9-22
references.pmid: 16369945
references.citation: Cell. 2001 Sep 7;106(5):585-94
references.pmid: 11551506
references.citation: J Bacteriol. 2009 Apr;191(8):2843-50
references.pmid: 19218378
references.citation: Environ Microbiol. 2005 Aug;7(8):1065-73
references.pmid: 16011745
references.citation: J Bacteriol. 1989 Nov;171(11):6271-8
references.pmid: 2681160
references.citation: J Fish Dis. 2003 Feb;26(2):103-8
references.pmid: 12962218
references.citation: Nat Rev Mol Cell Biol. 2007 Feb;8(2):127-38
references.pmid: 17228330
references.citation: Infect Immun. 1992 Jun;60(6):2475-80
references.pmid: 1587617
references.citation: Nature. 2001 Oct 25;413(6858):852-6
references.pmid: 11677609
references.citation: PLoS One. 2009;4(3):e4975
references.pmid: 19305502
references.citation: Nucleic Acids Res. 2000 Nov 15;28(22):E97
references.pmid: 11071951
references.citation: Mol Microbiol. 2005 Oct;58(2):520-32
references.pmid: 16194237
references.citation: J Bacteriol. 2002 Nov;184(22):6235-49
references.pmid: 12399494
references.citation: Crit Rev Biochem Mol Biol. 2007 Jan-Feb;42(1):41-63
references.pmid: 17364684
references.citation: Microbiology (Reading). 1994 May;140 ( Pt 5):1217-22
references.pmid: 8025688
references.citation: BMC Biol. 2007 Mar 28;5:14
references.pmid: 17391508
references.citation: Biochimie. 1982 Aug-Sep;64(8-9):669-75
references.pmid: 6291636
references.citation: Mol Gen Genet. 1982;187(1):4-11
references.pmid: 6761542
references.citation: Annu Rev Microbiol. 2005;59:357-77
references.pmid: 16153173
references.citation: Infect Immun. 2002 Aug;70(8):4721-5
references.pmid: 12117991
references.citation: Virology. 2006 Aug 1;351(2):360-7
references.pmid: 16713610
references.citation: J Exp Med. 1994 Jul 1;180(1):15-23
references.pmid: 8006579
references.citation: Biochimie. 1991 Apr;73(4):411-21
references.pmid: 1911941
references.citation: Microbes Infect. 2001 Nov-Dec;3(14-15):1345-52
references.pmid: 11755424
references.citation: Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):3777-81
references.pmid: 17360429
references.citation: Microbiol Rev. 1984 Mar;48(1):60-93
references.pmid: 6371470
references.citation: J Food Sci. 2007 Mar;72(2):M72-8
references.pmid: 17995846
references.citation: Mol Microbiol. 2003 Aug;49(3):769-81
references.pmid: 12864858
references.citation: Annu Rev Genet. 1997;31:213-44
references.pmid: 9442895
references.citation: Infect Immun. 1999 Apr;67(4):1614-22
references.pmid: 10084994
references.citation: Int J Food Microbiol. 2009 Aug 31;134(1-2):104-12
references.pmid: 19356819
references.citation: Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5
references.pmid: 10829079
references.citation: Nat Rev Microbiol. 2009 Jan;7(1):73-80
references.pmid: 19079353
references.citation: Mol Microbiol. 1999 Jul;33(1):167-76
references.pmid: 10411733
references.citation: J Bacteriol. 1986 Sep;167(3):1055-7
references.pmid: 3528124
references.citation: J Biol Chem. 1998 Mar 6;273(10):5708-15
references.pmid: 9488703
references.citation: Infect Immun. 1992 Sep;60(9):3596-600
references.pmid: 1500167
references.citation: Mol Microbiol. 2006 Jun;60(6):1590-602
references.pmid: 16796690
references.citation: Biomed Res. 2008 Feb;29(1):9-18
references.pmid: 18344593
references.citation: J Mol Biol. 2009 Jul 3;390(1):1-9
references.pmid: 19422832
references.citation: Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8631-5
references.pmid: 8078935
references.citation: J Bacteriol. 1972 Nov;112(2):886-93
references.pmid: 4343824
references.citation: Genome Res. 2006 May;16(5):686-91
references.pmid: 16606699
references.citation: J Clin Invest. 1995 Mar;95(3):1047-53
references.pmid: 7883952
references.citation: Mol Microbiol. 2010 Mar;75(5):1171-81
references.pmid: 20487303
references.citation: FEMS Microbiol Rev. 2007 Nov;31(6):637-56
references.pmid: 17883408
references.citation: J Mol Biol. 1990 Mar 5;212(1):79-96
references.pmid: 2108251
references.citation: Mol Microbiol. 1993 Mar;7(6):933-6
references.pmid: 8387147
references.citation: Diagn Microbiol Infect Dis. 2006 Apr;54(4):315-7
references.pmid: 16466895
references.citation: Bioessays. 1996 Sep;18(9):757-65
references.pmid: 8831292