Simultaneous biosynthesis of two copolymers in Pseudomonas putida GPo1 using a two-stage continuous culture system.
The bacterial production of medium-chain-length polyhydroxyalkanoate (PHA) in continuous two-stage cultivation was investigated. Two chemostats were linked in sequence and Pseudomonas putida GPo1 was supplied with different concentrations of octanoic acid in the first and 10-undecenoic acid in the second reactor. PHA isolated from the second bioreactor was a blend of two copolymers typically consisting of poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) (PHO) and poly(3-hydroxy-10-undecenoate-co-3-hydroxy-8-nonenoate-co-3-hydroxy-6-heptenoate) (PHUE) with structural (monomeric) purities of 85-95 mol %, indicating simultaneous PHA accumulation and degradation inside the cell. The maximum PHA content was found to be 53.8% of total cell dry weight, which is very high for mcl-PHA.
Hartmann
René
R
Empa, Swiss Federal Laboratories for Materials Testing and Research, Laboratory for Biomaterials, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland.
Hany
Roland
R
Witholt
Bernard
B
Zinn
Manfred
M
D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media; D004867:Equipment Design; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D054813:Polyhydroxyalkanoates