title: Porphyrin-nanosensor conjugates. New tools for the measurement of intracellular response to reactive oxygen species.
abstract: Reactive oxygen species (ROS) have for some time been implicated in the onset and progression of medical conditions including cancer, ageing, heart disease and Alzheimer's disease. Recently, it has been postulated that ROS play a much more subtle role in intracellular signalling mechanisms as second messengers. Given the importance of these species in influencing cellular processes, it is surprising that tools for studying intracellular levels of ROS are extremely limited and devices for studying the cells' response to internally generated ROS are virtually non-existent. In order to study the response of cells to intracellular ROS we have designed a nano-scale device that can both generate ROS and simultaneously monitor the cells' reaction as a function of changes in the important signalling ion, calcium. Here we report the synthesis, characterisation, and calibration of a new ROS nano-probe and demonstrate its ability to detect cellular response to elevated levels of intracellular ROS.
authors.affiliation: Department of Chemistry, University of Hull, Kingston-upon-Hull, HU6 7RX, UK.
authors.forename: Leanne B
authors.initials: LB
authors.lastname: Josefsen
authors.forename: Jonathan W
authors.initials: JW
authors.lastname: Aylott
authors.forename: Andrew
authors.initials: A
authors.lastname: Beeby
authors.forename: Philip
authors.initials: P
authors.lastname: Warburton
authors.forename: John P
authors.initials: JP
authors.lastname: Boyle
authors.forename: Chris
authors.initials: C
authors.lastname: Peers
authors.forename: Ross W
authors.initials: RW
authors.lastname: Boyle
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D015374:Biosensing Techniques; D002118:Calcium / Q000737:chemistry; D002460:Cell Line; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry* / Q000378:metabolism; D015290:Second Messenger Systems; D015398:Signal Transduction; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D002118:Calcium; D011166:Porphyrins; D017382:Reactive Oxygen Species; D000099166:Polyacrylamides