[Effect of fipronil on apoptosis rate and Bcl-2 of PC12 cells].
OBJECTIVE
To study the apoptosis and mechanism of Fipronil on PC12 Cells.


METHODS
The effect of fipronil on the apoptosis and necrosis of PC12 cells of 3.13 x 10(-6), 1.25 x 10(-5) and 5.00 x 10(-5) mol/L three dose groups after 24 h treatment was detected by morphology and the apoptosis rate was detected by flow cytometer (FCM). The expression of Bcl-2 protein in PC12 cells of 3.13 x 10(-6), 1.25 x 10(-5) and 5.00 x 10(-5) mol/L three dose groups after 24 h treatment was measured by immunofluorescence.


RESULTS
The number of apoptotic cells of 3.13 x 10(-6) mol/L group was more than the control group examined by fluorescence microscope, and the number of dead cells of 5.00 x 10(-5) mol/L group was more than the control group. The apoptotic rates of PC12 cells was higher in the 3.13 x 10(-6) mol/L group than the control group measured by FCM, and the dead rates of PC12 cells was higher in the 5.00 x 10(-5) mol/L group than the control group (P < 0.05). Immunofluorescence cytochemistry experiment demonstrated that the level of Bcl-2 expression was significantly lower in the 3.13 x 10(-6) mol/L group than the control group.


CONCLUSIONS
At low dosage, fipronil increases the apoptotic rates of PC12 cells possibly by decreasing the expression of Bcl-2 protein while at high dosage, fipronil only increases the amount of necrotic cells.
Hu
Xiong-Fei
XF
Department of Health Toxicology and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
Li
Jing
J
Zhuang
Wan
W
Li
Long
L
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D016716:PC12 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011720:Pyrazoles / Q000633:toxicity*; D051381:Rats
D019253:Proto-Oncogene Proteins c-bcl-2; D011720:Pyrazoles; C082360:fipronil