title: Electrospun nanofibrous mats containing quaternized chitosan and polylactide with in vitro antitumor activity against HeLa cells.
abstract: Nanofibrous materials containing the antitumor drug doxorubicin hydrochloride (DOX) were easily prepared using a one-step method by electrospinning of DOX/poly(L-lactide-co-D,L-lactide) (coPLA) and DOX/quaternized chitosan (QCh)/coPLA solutions. The pristine and DOX-containing mats were characterized by ATR-FTIR and X-ray photoelectron spectroscopy (XPS). The release rate of DOX from the prepared fibers increased with the increase in DOX content. The DOX release process was diffusion-controlled. MTT cell viability studies revealed that incorporation of DOX and QCh in the nanofibrous mats led to a significant reduction in the HeLa cells viability. It was found, that the antitumor efficacy of the DOX-containing mats at 6 h was higher than that of the free DOX. SEM, TEM, and fluorescence microscopic observations confirmed that the antitumor effect of QCh-based and DOX-containing fibrous mats was mainly due to induction of apoptosis in the HeLa cells.
authors.affiliation: Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St. bl. 103A, BG-1113 Sofia, Bulgaria.
authors.forename: Milena G
authors.initials: MG
authors.lastname: Ignatova
authors.forename: Nevena E
authors.initials: NE
authors.lastname: Manolova
authors.forename: Reneta A
authors.initials: RA
authors.lastname: Toshkova
authors.forename: Iliya B
authors.initials: IB
authors.lastname: Rashkov
authors.forename: Elena G
authors.initials: EG
authors.lastname: Gardeva
authors.forename: Lilia S
authors.initials: LS
authors.lastname: Yossifova
authors.forename: Marin T
authors.initials: MT
authors.lastname: Alexandrov
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D055664:Electrochemical Techniques; D006367:HeLa Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D057139:Nanofibers / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; C118071:N-trimethyl chitosan chloride; D011091:Polyesters; C033616:poly(lactide); D004317:Doxorubicin; D048271:Chitosan