title: Quantitative proteomics analysis of the nuclear fraction of human CD4+ cells in the early phases of IL-4-induced Th2 differentiation.
abstract: We used stable isotope labeling with 4-plex iTRAQ (isobaric tags for relative and absolute quantification) reagents and LC-MS/MS to investigate proteomic changes in the nucleus of activated human CD4(+) cells during the early stages of Th2 cell differentiation. The effects of IL-4 stimulation upon activated naïve CD4(+) cells were measured in the nuclear fractions from 6 and 24 h in three biological replicates, each using pooled cord blood samples derived from seven or more individuals. In these analyses, in the order of 800 proteins were detected with two or more peptides and quantified in three biological replicates. In addition to consistent differences observed with the nuclear localization/expression of established human Th2 and Th1 markers, there were changes that suggested the involvement of several proteins either only recently reported or otherwise not known in this context. These included SATB1 and among the novel changes detected and validated an IL-4-induced increase in the level of YB1. This unique data set from human cord blood CD4(+) T cells details an extensive list of protein determinations that compares with and complements previous data determined from the Jurkat cell nucleus.
authors.affiliation: Turku Centre for Biotechnology, Turku, Finland.
authors.forename: Robert
authors.initials: R
authors.lastname: Moulder
authors.forename: Tapio
authors.initials: T
authors.lastname: Lönnberg
authors.forename: Laura L
authors.initials: LL
authors.lastname: Elo
authors.forename: Jan-Jonas
authors.initials: JJ
authors.lastname: Filén
authors.forename: Eeva
authors.initials: E
authors.lastname: Rainio
authors.forename: Garry
authors.initials: G
authors.lastname: Corthals
authors.forename: Matej
authors.initials: M
authors.lastname: Oresic
authors.forename: Tuula A
authors.initials: TA
authors.lastname: Nyman
authors.forename: Tero
authors.initials: T
authors.lastname: Aittokallio
authors.forename: Riitta
authors.initials: R
authors.lastname: Lahesmaa
mesh_terms: D015153:Blotting, Western; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects*; D002467:Cell Nucleus / Q000737:chemistry*; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry; D018418:Th2 Cells / Q000737:chemistry* / Q000166:cytology
chemical_list: D015847:Interleukin-4
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