[Molecular genetic analysis of Pseudomonas aeruginosa strains isolated from environment and patients in health care facilities].
OBJECTIVE
Analysis of genetic heterogeneity of Pseudomonas aeruginosa strains isolated in and out of hospitals.


METHODS
To study the genetic diversity of 36 strains of P. aeruginosa plasmid analysis, random amplified polymorphic DNA (RAPD) technique as well as polymerase chain reaction for detection of virulence genes algD, lasB, toxA, plcH, plcN, exoS, nan1, and nan2.


RESULTS
Epidemically important strains were found in different ecological niches. It was shown that these virulence factors could play important roles in pathogenesis of infection.


CONCLUSIONS
RAPD technique was effective for analysis of P. aeruginosa isolates. Number of studied typing bands differed between related isolates for each random primer.
Antonov
V A
VA
Altukhova
V V
VV
Savchenko
S S
SS
Tkachenko
G A
GA
Zamaraev
V S
VS
Zhukova
S I
SI
Kramar'
O G
OG
Matveeva
N L
NL
Ostrovskiĭ
O V
OV
Dudchenko
G P
GP
D017931:DNA Primers; D006801:Humans; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique; D012426:Russia / Q000453:epidemiology; D012680:Sensitivity and Specificity; D014505:Urban Population; D037521:Virulence Factors / Q000235:genetics
D017931:DNA Primers; D037521:Virulence Factors