Calcium activates a chloride conductance likely involved in olfactory receptor neuron repolarization in the moth Spodoptera littoralis.
The response of insect olfactory receptor neurons (ORNs) to odorants involves the opening of Ca(2+)-permeable channels, generating an increase in intracellular Ca(2+) concentration. Here, we studied the downstream effect of this Ca(2+) rise in cultured ORNs of the moth Spodoptera littoralis. Intracellular dialysis of Ca(2+) from the patch pipette in whole-cell patch-clamp configuration activated a conductance with a K(1/2) of 2.8 microm. Intracellular and extracellular anionic and cationic substitutions demonstrated that Cl(-) carries this current. The anion permeability sequence I(-) > NO(3)(-) > Br(-) > Cl(-) > CH(3)SO(3)(-) >> gluconate(-) of the Ca(2+)-activated Cl(-) channel suggests a weak electrical field pore of the channel. The Ca(2+)-activated current partly inactivated over time and did not depend on protein kinase C (PKC) and CaMKII activity or on calmodulin. Application of Cl(-) channel blockers, flufenamic acid, 5-nitro-2-(3-phenylpropylamino) benzoic acid, or niflumic acid reversibly blocked the Ca(2+)-activated current. In addition, lowering Cl(-) concentration in the sensillar lymph bathing the ORN outer dendrites caused a significant delay in pheromone response termination in vivo. The present work identifies a new Cl(-) conductance activated by Ca(2+) in insect ORNs presumably required for ORN repolarization.
Pézier
Adeline
A
Unité Mixte de Recherche 1272 Physiologie de l'Insecte, Signalisation et Communication, Institut National de la Recherche Agronomique, F-78000 Versailles, France.
Grauso
Marta
M
Acquistapace
Adrien
A
Monsempes
Christelle
C
Rospars
Jean-Pierre
JP
Lucas
Philippe
P
D000818:Animals; D000838:Anions / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology / Q000502:physiology*; D020013:Calcium Signaling / Q000187:drug effects; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000037:antagonists & inhibitors; D002147:Calmodulin / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D049990:Membrane Transport Modulators / Q000494:pharmacology; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000502:physiology*; D010539:Permeability; D010675:Pheromones / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D018411:Spodoptera / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D000838:Anions; D002147:Calmodulin; D018118:Chloride Channels; D004791:Enzyme Inhibitors; D049990:Membrane Transport Modulators; D010675:Pheromones; D011493:Protein Kinase C; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D002118:Calcium
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