title: Short- and long-term memory are modulated by multiple isoforms of the fragile X mental retardation protein.
abstract: The diversity of protein isoforms arising from alternative splicing is thought to modulate fine-tuning of synaptic plasticity. Fragile X mental retardation protein (FMRP), a neuronal RNA binding protein, exists in isoforms as a result of alternative splicing, but the contribution of these isoforms to neural plasticity are not well understood. We show that two isoforms of Drosophila melanogaster FMRP (dFMR1) have differential roles in mediating neural development and behavior functions conferred by the dfmr1 gene. These isoforms differ in the presence of a protein interaction module that is related to prion domains and is functionally conserved between FMRPs. Expression of both isoforms is necessary for optimal performance in tests of short- and long-term memory of courtship training. The presence or absence of the protein interaction domain may govern the types of ribonucleoprotein (RNP) complexes dFMR1 assembles into, with different RNPs regulating gene expression in a manner necessary for establishing distinct phases of memory formation.
authors.affiliation: Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.
authors.forename: Paromita
authors.initials: P
authors.lastname: Banerjee
authors.forename: Brian P
authors.initials: BP
authors.lastname: Schoenfeld
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Bell
authors.forename: Catherine H
authors.initials: CH
authors.lastname: Choi
authors.forename: Michael P
authors.initials: MP
authors.lastname: Bradley
authors.forename: Paul
authors.initials: P
authors.lastname: Hinchey
authors.forename: Maria
authors.initials: M
authors.lastname: Kollaros
authors.forename: Jae H
authors.initials: JH
authors.lastname: Park
authors.forename: Sean M J
authors.initials: SM
authors.lastname: McBride
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Dockendorff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002940:Circadian Rhythm / Q000502:physiology; D004252:DNA Mutational Analysis; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D005260:Female; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008568:Memory / Q000502:physiology*; D008570:Memory, Short-Term / Q000502:physiology*; D008969:Molecular Sequence Data; D009043:Motor Activity / Q000502:physiology; D009483:Neuropsychological Tests; D020033:Protein Isoforms / Q000378:metabolism; D012726:Sexual Behavior, Animal / Q000502:physiology; D013997:Time Factors
chemical_list: D029721:Drosophila Proteins; D051860:Fragile X Messenger Ribonucleoprotein 1; D020033:Protein Isoforms; C467328:FMR1 protein, Drosophila
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