title: Systematic genetic analysis of the Plasmodium falciparum MSP7-like family reveals differences in protein expression, location, and importance in asexual growth of the blood-stage parasite.
abstract: Proteins located on Plasmodium falciparum merozoites, the invasive form of the parasite's asexual blood stage, are of considerable interest in vaccine research. Merozoite surface protein 7 (MSP7) forms a complex with MSP1 and is encoded by a member of a multigene family located on chromosome 13. The family codes for MSP7 and five MSP7-related proteins (MSRPs). In the present study, we have investigated the expression and the effect of msrp gene deletion at the asexual blood stage. In addition to msp7, msrp2, msrp3, and msrp5 are transcribed, and mRNA was easily detected by hybridization analysis, whereas mRNA for msrp1 and msrp4 could be detected only by reverse transcription (RT)-PCR. Notwithstanding evidence of transcription, antibodies to recombinant MSRPs failed to detect specific proteins, except for antibodies to MSRP2. Sequential proteolytic cleavages of MSRP2 resulted in 28- and 25-kDa forms. However, MSRP2 was absent from merozoites; the 25-kDa MSRP2 protein (MSRP2(25)) was soluble and secreted upon merozoite egress. The msrp genes were deleted by targeted disruption in the 3D7 line, leading to ablation of full-length transcripts. MSRP deletion mutants had no detectable phenotype, with growth and invasion characteristics comparable to those of the parental parasite; only the deletion of MSP7 led to a detectable growth phenotype. Thus, within this family some of the genes are transcribed at a significant level in asexual blood stages, but the corresponding proteins may or may not be detectable. Interactions of the expressed proteins with the merozoite also differ. These results highlight the potential for unexpected differences of protein expression levels within gene families.
authors.affiliation: Division of Parasitology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom. mkadeko@nimr.mrc.ac.uk
authors.forename: Madhusudan
authors.initials: M
authors.lastname: Kadekoppala
authors.forename: Solabomi A
authors.initials: SA
authors.lastname: Ogun
authors.forename: Steven
authors.initials: S
authors.lastname: Howell
authors.forename: Ruwani S
authors.initials: RS
authors.lastname: Gunaratne
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Holder
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000469:parasitology; D017353:Gene Deletion; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques; D017125:Genes, Protozoan; D056426:Genetic Loci / Q000235:genetics; D006801:Humans; D008018:Life Cycle Stages / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D010963:Plasmodium falciparum / Q000235:genetics* / Q000254:growth & development*; D021381:Protein Transport; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D012100:Reproduction, Asexual / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D008565:Membrane Proteins; D015800:Protozoan Proteins; C439571:merozoite surface protein 7, Plasmodium
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