title: Effect of human beta-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models.
abstract: The purpose of the present study was to investigate the efficiency of embryo cryopreservation for four transgenic (TG) thalassaemic mouse strains, which is a key element of the ongoing gene banking efforts for these high-value animals. Heterozygous TG embryos were produced by breeding four lines of TG males to wild-type (WT) females (C57BL/6J). Intact two-cell embryos were cryopreserved by vitrification in straws using 35% ethylene glycol. Survival rates of cryopreserved embryos ranged between 91.1% (102/112) and 93.6% (176/188) without significant differences between the lines. In contrast, the paternal line had a significant effect on the development of these embryos to the blastocyst stage, which ranged from 50.6% (92/182) to 77.5% (79/102). This effect was also noted following embryo transfers, with implantation rates varying from 17.3% (19/110) to 78.1% (35/45). The results demonstrate that the in vivo developmental potential is significantly influenced by TG line and reveal a specific line effect on cryosurvival. All bacterial artificial chromosome transgenic fetuses developed from vitrified-warmed embryos showed expression of the human beta-globin transgene. In conclusion, the present study shows a strong TG line effect on developmental competence following cryopreservation and the vitrification method was successful to bank the human beta-globin TG-expressing mouse strains.
authors.affiliation: Department of Biology, Faculty of Science, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand.
authors.forename: Duangjai
authors.initials: D
authors.lastname: Boonkusol
authors.forename: Andras
authors.initials: A
authors.lastname: Dinnyes
authors.forename: Tassanee
authors.initials: T
authors.lastname: Faisaikarm
authors.forename: Parisatcha
authors.initials: P
authors.lastname: Sangsuwan
authors.forename: Nathnapith
authors.initials: N
authors.lastname: Pratipnatalang
authors.forename: Mayurachat
authors.initials: M
authors.lastname: Sa-Ardrit
authors.forename: Kulnasan
authors.initials: K
authors.lastname: Saikhun
authors.forename: Saovaros
authors.initials: S
authors.lastname: Svasti
authors.forename: Jim
authors.initials: J
authors.lastname: Vadolas
authors.forename: Pranee
authors.initials: P
authors.lastname: Winichagoon
authors.forename: Suthat
authors.initials: S
authors.lastname: Fucharoen
authors.forename: Yindee
authors.initials: Y
authors.lastname: Kitiyanant
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D015925:Cryopreservation; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006579:Heterozygote; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D008822:Mice, Transgenic; D013789:Thalassemia / Q000235:genetics; D055544:beta-Globins / Q000235:genetics*
chemical_list: D055544:beta-Globins