Inactivation and augmentation of the primary 3-ketosteroid-{delta}1- dehydrogenase in Mycobacterium neoaurum NwIB-01: biotransformation of soybean phytosterols to 4-androstene- 3,17-dione or 1,4-androstadiene-3,17-dione.
3-Ketosteroid-Delta(1)-dehydrogenase, KsdD(M), was identified by targeted gene disruption and augmentation from Mycobacterium neoaurum NwIB-01, a newly isolated strain. The difficulty of separating 4-androstene-3,17-dione (AD) from 1,4-androstadiene-3,17-dione (ADD) is a key bottleneck to the microbial transformation of phytosterols in industry. This problem was tackled via genetic manipulation of the KsdD-encoding gene. Mutants in which KsdD(M) was inactivated or augmented proved to be good AD(D)-producing strains.
Wei
Wei
W
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
Wang
Feng-Qing
FQ
Fan
Shu-Yue
SY
Wei
Dong-Zhi
DZ
D000595:Amino Acid Sequence; D000730:Androstadienes / Q000378:metabolism*; D000735:Androstenedione / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D003001:Cloning, Molecular; D017353:Gene Deletion; D005818:Genetic Engineering / Q000379:methods; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010840:Phytosterols / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D013025:Glycine max / Q000378:metabolism*
D000730:Androstadienes; D010840:Phytosterols; C006573:1,4-androstadiene-3,17-dione; D000735:Androstenedione; D010088:Oxidoreductases; C022656:3-oxosteroid delta(1) dehydrogenase
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