[Cloning and characterization of an aldehyde dehydrogenase gene GmALDH3-1 from soybean].
To research the mechanism of soybean reproductive development, we identified a number of flower development related genes in soybean by microarray hybridization. A gene predominately expressed in soybean flowers was chosen for further analysis. Through bioinformatic and RT-PCR approaches, the full-length gene was cloned from soybean flowers. The results of BLAST searching indicated that this gene encoded for an aldehyde dehydrogenase and was named as GmALDH3-1. GmALDH3-1 contains a complete open reading frame of 1485 bp in length, which encodes for a peptide of 494 amino acids. The product encoded by GmALDH3-1 shows 83% similarity and 68% identity with Populus tomentosa PtALDH3, respectively, and 39% and 59% with human ALDH3B. Phylogenetic analysis shows that GmALDH3-1 and other ALDH3 subfamily members are grouped into the same branch and GmALDH3-1 is close to PtALDH3 and Arabidopsis AtALDH3F1. Real-time RT-PCR analysis demonstrated that the highest expression level of GmALDH3-1 occurred in flowers, but the expression of this gene was almost undetectable in leaves and roots. We further analyzed GmALDH3-1 expression during the course of seed development based on publicly available microarray data and found that GmALDH3-1 was highly expressed in the seed endothelium, epidermis, outer integument, and hilum.
Huang
Fang
F
National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. fhuang@njau.edu.cn
Chi
Ying-Jun
YJ
He
Hui
H
Yu
De-Yue
DY
D000444:Aldehyde Dehydrogenase / Q000145:classification / Q000235:genetics*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D035264:Flowers / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D030262:Soybean Proteins / Q000235:genetics*; D013025:Glycine max / Q000201:enzymology / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins; D030262:Soybean Proteins; C553864:ALDH3-1 protein, soybean; D000444:Aldehyde Dehydrogenase