Human selenophosphate synthetase 1 has five splice variants with unique interactions, subcellular localizations and expression patterns.
Selenophosphate synthetase 1 (SPS1) is an essential cellular gene in higher eukaryotes. Five alternative splice variants of human SPS1 (major type, DeltaE2, DeltaE8, +E9, +E9a) were identified wherein +E9 and +E9a make the same protein. The major type was localized in both the nuclear and plasma membranes, and the others in the cytoplasm. All variants form homodimers, and in addition, the major type forms a heterodimer with DeltaE2, and DeltaE8 with +E9. The level of expression of each splice variant was different in various cell lines. The expression of each alternative splice variant was regulated during the cell cycle. The levels of the major type and DeltaE8 were gradually increased until G2/M phase and then gradually decreased. DeltaE2 expression peaked at mid-S phase and then gradually decreased. However, +E9/+E9a expression decreased gradually after cell cycle arrest. The possible involvement of SPS1 splice variants in cell cycle regulation is discussed.
Kim
Jin Young
JY
Laboratory of Molecular Genetics and Genomics, School of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Republic of Korea.
Lee
Kwang Hee
KH
Shim
Myoung Sup
MS
Shin
Hyein
H
Xu
Xue-Ming
XM
Carlson
Bradley A
BA
Hatfield
Dolph L
DL
Lee
Byeong Jae
BJ
D017398:Alternative Splicing; D002453:Cell Cycle; D002460:Cell Line; D006367:HeLa Cells; D006801:Humans; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D010770:Phosphotransferases; C062762:selenophosphate synthetase
Protein Sci. 2008 Jan;17(1):176-82
18156471
J Biol Chem. 1991 Apr 5;266(10):6324-8
2007585
Proc Natl Acad Sci U S A. 1996 Dec 24;93(26):15086-91
8986768
Biochem J. 2007 May 15;404(1):115-20
17346238
Proc Natl Acad Sci U S A. 1990 Jan;87(2):543-7
2405383
J Biol Chem. 1995 Sep 15;270(37):21659-64
7665581
PLoS Biol. 2007 Jan;5(1):e4
17194211
Dev Biol. 2001 Oct 1;238(1):145-56
11784000
Antioxid Redox Signal. 2007 Jul;9(7):775-806
17508906
Biol Chem. 2007 Oct;388(10):987-95
17937612
Mol Gen Genet. 1998 Jan;257(2):113-23
9491069
Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16162-7
15534230
J Biol Chem. 1989 Jun 15;264(17):9720-3
2524495
J Biol Chem. 2009 Nov 20;284(47):32881-94
19755423
Eur J Biochem. 1992 Jun 15;206(3):767-73
1606960
J Cell Sci. 2003 Nov 15;116(Pt 22):4597-604
14576353
J Biol Chem. 1989 Jun 15;264(17):9724-7
2498338
Biol Trace Elem Res. 2007 Dec;119(3):234-41
17916946
Nutr Clin Pract. 2008 Apr-May;23(2):152-60
18390782
J Mol Biol. 2009 Jul 24;390(4):747-59
19477186
Biochemistry. 1993 Nov 30;32(47):12555-9
8251472
Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12848-53
15317934
J Cell Sci. 1999 Sep;112 ( Pt 17):2875-84
10444382
Mol Cell Biol. 2006 Mar;26(6):2337-46
16508009
J Natl Cancer Inst. 2004 Apr 7;96(7):504-5
15069108