Regional divergence of phospholipase A(2)-like protein cDNAs between New Guinean and Australian Pseudechis australis.
Snake Phospholipase A(2) (PLA(2)) exhibits diverse pharmacological effects, such as hemolysis, myotoxicity, and neurotoxicity. In this study, we identified 10 novel PLA(2)-like protein cDNAs, which we named Pr 1-10, from the venom gland cDNA library of Papuan pigmy mulga snake (New Guinean Pseudechis australis). The deduced amino acid sequence of Pr 1, which ortholog has not been reported in mulaga snake (Australian P. australis) yet, shows 78.8% identity with the ortholog in Australian tiger snake (Notechis scutatus scutatus). The amino acid sequences of Pr 2-10 are 92.4-99.3% identical with their orthologs and paralogs in Australian P. australis.
Inagaki
Hidetoshi
H
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. h-inagaki@aist.go.jp
Yamauchi
Yoko
Y
Toriba
Michihisa
M
Kubo
Tai
T
D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000737:chemistry; D004546:Elapid Venoms / Q000737:chemistry* / Q000235:genetics; D017815:Elapidae / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D014644:Genetic Variation; D054467:Phospholipases A2 / Q000737:chemistry* / Q000235:genetics; D010802:Phylogeny; D030162:Reptilian Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
D018076:DNA, Complementary; D004546:Elapid Venoms; D030162:Reptilian Proteins; D054467:Phospholipases A2