title: A highly selective fluorescent probe for detection of biological samples thiol and its application in living cells.
abstract: Probe 1 was designed and synthesized as a new fluorescent molecular probe for thiols in PBS buffer at physiological condition. This fluorescent molecular probe consists of a thiol reaction moiety bound to a coumarin fluorophore. Its fluorescence quantum yield is low, but a drastic enhancement of fluorescence intensity was observed in the presence of thiols. Possible interference with other analytes was examined. Probe 1 displays a highly selective fluorescent enhancement with thiols, and the probe was successfully applied to thiols determination in intracellular, in human urine and blood samples.
authors.affiliation: Pharmaceutics Department, College of pharmacy, Central South University, Changsha, Hunan, 410013, People's Republic of China.
authors.forename: Qing-Ping
authors.initials: QP
authors.lastname: Zuo
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Pei
authors.forename: Zuojun
authors.initials: Z
authors.lastname: Li
authors.forename: Shi-Kun
authors.initials: SK
authors.lastname: Liu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D011789:Quantum Theory; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000032:analysis*; D014062:Tongue Neoplasms / Q000737:chemistry* / Q000473:pathology
chemical_list: D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds
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