Reprogramming bacteria to seek and destroy an herbicide.
A major goal of synthetic biology is to reprogram cells to perform complex tasks. Here we show how a combination of in vitro and in vivo selection rapidly identifies a synthetic riboswitch that activates protein translation in response to the herbicide atrazine. We further demonstrate that this riboswitch can reprogram bacteria to migrate in the presence of atrazine. Finally, we show that incorporating a gene from an atrazine catabolic pathway allows these cells to seek and destroy atrazine.
Sinha
Joy
J
Department of Chemistry and Center for Fundamental and Applied Molecular Evolution, Emory University, Atlanta, Georgia, USA.
Reyes
Samuel J
SJ
Gallivan
Justin P
JP
D000096:Acetone / Q000378:metabolism; D001280:Atrazine / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D018407:Bacterial Physiological Phenomena; D001483:Base Sequence; D002465:Cell Movement; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004983:Ethanolamines / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006540:Herbicides / Q000138:chemical synthesis / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D004269:DNA, Bacterial; D004983:Ethanolamines; D006540:Herbicides; D012329:RNA, Bacterial; D000096:Acetone; D001616:beta-Galactosidase; D001280:Atrazine
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