title: Laser scanning microscopy combined with image restoration to analyse a 3D model of the human epithelial airway barrier.
abstract: A laser scanning microscope collects information from a thin, focal plane and ignores out of focus information. During the past few years it has become the standard imaging method to characterise cellular morphology and structures in static as well as in living samples. Laser scanning microscopy combined with digital image restoration is an excellent tool for analysing the cellular cytoarchitecture, expression of specific proteins and interactions of various cell types, thus defining valid criteria for the optimisation of cell culture models. We have used this tool to establish and evaluate a three dimensional model of the human epithelial airway wall.
authors.affiliation: Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland. rothen@ana.unibe.ch
authors.forename: Barbara
authors.initials: B
authors.lastname: Rothen-Rutishauser
authors.forename: Andrea D
authors.initials: AD
authors.lastname: Lehmann
authors.forename: Martin J D
authors.initials: MJ
authors.lastname: Clift
authors.forename: Fabian
authors.initials: F
authors.lastname: Blank
authors.forename: Peter
authors.initials: P
authors.lastname: Gehr
mesh_terms: D002450:Cell Communication / Q000502:physiology; D018920:Coculture Techniques; D003198:Computer Simulation; D003713:Dendritic Cells / Q000648:ultrastructure; D004847:Epithelial Cells / Q000648:ultrastructure; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008264:Macrophages / Q000648:ultrastructure; D018613:Microscopy, Confocal / Q000379:methods*; D020545:Respiratory Mucosa / Q000033:anatomy & histology*