Spatiotemporal Regulation of Signaling in and out of Dendritic Spines: CaMKII and Ras.
Recent advances in 2-photon fluorescence lifetime imaging microscopy (2pFLIM) in combination with 2-photon photochemistry have enabled the visualization of neuronal signaling during synaptic plasticity at the level of single dendritic spines in light scattering tissue. Using these techniques, the activity of Ca(2+)/Calmodulin-dependent kinase II (CaMKII) and Ras have been imaged in single spines during synaptic plasticity and associated spine enlargement. These provide two contrasting examples of spatiotemporal regulation of spine signaling: Ras signaling is diffusive and spread over ~10 μm along the dendrites, while CaMKII activation is restricted to the spine undergoing plasticity. In this review, we will discuss the mechanisms and roles of the different spatiotemporal regulation of signaling in neurons, and the impact of the spine structure upon these biochemical signaling processes.
Lee
Seok-Jin R
SJ
Department of Neurobiology, Duke University Medical Center, Durham, NC 27710.
Yasuda
Ryohei
R
Trends Pharmacol Sci. 2005 Dec;26(12):645-53
16253351
Annu Rev Physiol. 2002;64:313-53
11826272
FEBS J. 2006 Feb;273(4):682-94
16441656
Nature. 2009 Mar 19;458(7236):299-304
19295602
J Cell Biol. 1985 Mar;100(3):835-42
2982886
Hippocampus. 2002;12(5):667-79
12440581
Nat Neurosci. 1999 Jul;2(7):597-604
10404178
Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):341-6
18162541
Nature. 2000 Apr 20;404(6780):876-81
10786794
Cell Calcium. 1998 Nov-Dec;24(5-6):345-57
10091004
Neuron. 2004 Sep 30;44(1):5-21
15450156
J Neurosci. 2004 Feb 25;24(8):2054-64
14985448
Nat Neurosci. 2006 Feb;9(2):283-91
16429133
Curr Opin Neurobiol. 2001 Jun;11(3):349-56
11399434
Nature. 1999 May 13;399(6732):151-5
10335844
Neuron. 2004 Oct 14;44(2):351-64
15473972
Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):8366-71
12048251
Science. 2008 Jul 4;321(5885):136-40
18556515
J Neurosci. 2003 Jun 1;23(11):4428-36
12805283
Nature. 1999 May 6;399(6731):66-70
10331391
J Neurosci. 1997 Jul 15;17(14):5357-65
9204920
Cell. 2005 Dec 2;123(5):849-60
16325579
Neuron. 2004 Dec 2;44(5):759-67
15572108
Nat Rev Neurosci. 2004 Mar;5(3):173-83
14976517
Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6418-23
17404223
Cell. 1986 Mar 28;44(6):861-70
3006921
Curr Biol. 2008 Oct 28;18(20):1546-54
18929487
Biophys J. 2005 Jun;88(6):4319-36
15792970
Eur J Neurosci. 2004 Dec;20(11):3063-72
15579161
Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6369-74
19339497
Nature. 1984 Jul 5-11;310(5972):74-6
6330568
J Neurosci. 2008 Dec 10;28(50):13457-66
19074019
Nat Rev Neurosci. 2002 Mar;3(3):175-90
11994750
Acta Neuropathol. 1980;50(3):207-10
6447982
Nat Neurosci. 2004 Sep;7(9):917-8
15322548
Front Neural Circuits. 2008 Aug 13;2:2
18946542
Neuron. 2009 Feb 12;61(3):340-50
19217372
Neuron. 2008 Aug 14;59(3):359-74
18701063
Science. 1998 Feb 6;279(5352):870-3
9452388
J Cell Biol. 1991 Nov;115(4):1049-60
1659571
Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18799-804
17132736
Trends Neurosci. 2004 Feb;27(2):77-83
15102486
Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4089-94
16537489
Science. 2005 Nov 4;310(5749):866-9
16272125
Annu Rev Neurosci. 2007;30:79-97
17280523
Neuron. 2009 Feb 12;61(3):425-38
19217379
J Neurosci. 2008 Dec 10;28(50):13592-608
19074033
Annu Rev Physiol. 2009;71:261-82
19575680
Neuron. 2008 Dec 10;60(5):788-802
19081375
Nat Rev Neurosci. 2004 Dec;5(12):931-42
15550948
Nature. 2002 Apr 4;416(6880):535-9
11932745
Neuron. 2008 Mar 13;57(5):719-29
18341992
Annu Rev Neurosci. 2001;24:1071-89
11520928
J Neurosci. 2007 May 9;27(19):5190-9
17494705
Science. 1990 Apr 6;248(4951):73-6
2321027
Neuron. 2002 Jan 31;33(3):439-52
11832230
Science. 2002 Sep 13;297(5588):1873-7
12228718
Nature. 2004 Jun 17;429(6993):761-6
15190253
Science. 1997 Jun 27;276(5321):2042-5
9197267
Nature. 2001 Jun 14;411(6839):801-5
11459059
J Biol Chem. 1999 Sep 10;274(37):26199-208
10473573
Adv Biochem Eng Biotechnol. 2010;119:79-97
19499207
Science. 1989 Aug 25;245(4920):862-6
2549638
J Neurosci. 2000 Nov 15;20(22):8262-8
11069932
Cell. 2002 Aug 23;110(4):443-55
12202034
Nature. 2006 Apr 13;440(7086):935-9
16612384
J Cell Biol. 2005 Nov 7;171(3):537-47
16275756
Neuron. 2005 May 19;46(4):609-22
15944129
Science. 1999 Mar 19;283(5409):1923-7
10082466
Neuron. 2003 Nov 13;40(4):807-21
14622584
Neuron. 2001 Aug 2;31(2):191-201
11502252
Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17961-6
17093040
Annu Rev Biochem. 2007;76:823-47
17243894
Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):14080-4
19666491
Science. 2008 May 16;320(5878):903-9
18487186
Nat Rev Neurosci. 2005 Jun;6(6):423-34
15928715
J Neurol Neurosurg Psychiatry. 1998 Oct;65(4):446-53
9771764
Neuron. 2002 Sep 12;35(6):1019-27
12354393
Annu Rev Neurosci. 2008;31:47-67
18284372
Biophys J. 2006 Aug 1;91(3):1090-7
16920696
Acta Neuropathol. 1990;79(6):680-5
2141748
J Neurosci. 2007 Mar 14;27(11):2866-75
17360908
J Neurochem. 2000 Dec;75(6):2277-87
11080179
Neuron. 2004 Dec 2;44(5):749-57
15572107
Annu Rev Neurosci. 1994;17:341-71
8210179
Neuron. 2005 Oct 20;48(2):189-99
16242400
Neuron. 2006 Jun 15;50(6):823-39
16772166
J Biol Chem. 1998 Oct 23;273(43):28424-9
9774470
J Neurophysiol. 2001 Apr;85(4):1368-76
11287461
Nature. 1997 Aug 28;388(6645):882-7
9278050
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9497-501
3467320
Genes Dev. 2005 Sep 1;19(17):2000-15
16107614
Cell. 2008 Jun 27;133(7):1228-40
18585356
J Neurosci. 2007 Jun 20;27(25):6601-4
17581946
Nat Neurosci. 2001 Nov;4(11):1086-92
11687814
J Neurosci. 2005 Jun 29;25(26):6037-46
15987933
Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):4287-92
12646704
Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):11175-9
7479960
Brain Cell Biol. 2008 Aug;36(1-4):31-42
18512154
J Biol Chem. 1993 Apr 15;268(11):7863-7
8385124
Nat Neurosci. 2001 Feb;4(2):151-8
11175875
Nature. 2002 Dec 19-26;420(6917):788-94
12490942
Science. 1996 May 3;272(5262):716-9
8614831
Science. 2004 Apr 16;304(5669):432-5
15087548
J Biol Chem. 2009 Oct 9;284(41):28146-28155
19654320
Nature. 2009 Feb 26;457(7233):1128-32
19242475
Curr Opin Neurobiol. 2006 Oct;16(5):551-61
16971112
Brain Res Brain Res Rev. 2002 Jun;39(1):29-54
12086707
Nat Neurosci. 2004 Oct;7(10):1104-12
15361876
Nat Neurosci. 2006 Jun;9(6):798-806
16680164
Science. 2006 Sep 15;313(5793):1642-5
16902090
Nature. 1998 Apr 30;392(6679):936-41
9582076
Nat Rev Neurosci. 2002 Dec;3(12):921-31
12461549
Nat Methods. 2006 Oct;3(10):793-5
16896339
Annu Rev Physiol. 1995;57:417-45
7778873
J Neurosci. 2005 Dec 7;25(49):11288-99
16339024
J Neurosci. 1998 Nov 1;18(21):8900-11
9786995
J Cell Biol. 2008 Dec 1;183(5):849-63
19047462
Curr Opin Neurobiol. 1999 Jun;9(3):343-8
10395574
J Neurosci. 2007 Jan 24;27(4):796-807
17251419
Proc Natl Acad Sci U S A. 2004 May 11;101(19):7317-22
15123831
Front Neural Circuits. 2009 May 27;3:2
19506708
Nature. 1987 May 21-27;327(6119):249-52
3494954
Nat Neurosci. 2003 Sep;6(9):948-55
12937422
J Neurosci. 2005 Mar 23;25(12):3107-12
15788767
Neuron. 2008 Sep 25;59(6):882-901
18817729
Nature. 2007 Dec 20;450(7173):1195-200
18097401
J Neurosci. 2003 Nov 5;23(31):10116-21
14602827
Neuron. 2006 Dec 7;52(5):831-43
17145504