c-Fos expression during temporal order judgment in mice.
The neuronal mechanisms for ordering sensory signals in time still need to be clarified despite a long history of research. To address this issue, we recently developed a behavioral task of temporal order judgment in mice. In the present study, we examined the expression of c-Fos, a marker of neural activation, in mice just after they carried out the temporal order judgment task. The expression of c-Fos was examined in C57BL/6N mice (male, n = 5) that were trained to judge the order of two air-puff stimuli delivered bilaterally to the right and left whiskers with stimulation intervals of 50-750 ms. The mice were rewarded with a food pellet when they responded by orienting their head toward the first stimulus (n = 2) or toward the second stimulus (n = 3) after a visual "go" signal. c-Fos-stained cell densities of these mice (test group) were compared with those of two control groups in coronal brain sections prepared at bregma -2, -1, 0, +1, and +2 mm by applying statistical parametric mapping to the c-Fos immuno-stained sections. The expression of c-Fos was significantly higher in the test group than in the other groups in the bilateral barrel fields of the primary somatosensory cortex, the left secondary somatosensory cortex, the dorsal part of the right secondary auditory cortex. Laminar analyses in the primary somatosensory cortex revealed that c-Fos expression in the test group was most evident in layers II and III, where callosal fibers project. The results suggest that temporal order judgment involves processing bilateral somatosensory signals through the supragranular layers of the primary sensory cortex and in the multimodal sensory areas, including marginal zone between the primary somatosensory cortex and the secondary sensory cortex.
Wada
Makoto
M
Department of Physiology, Juntendo University School of Medicine, Tokyo, Japan. m-wada@juntendo.ac.jp
Higo
Noriyuki
N
Moizumi
Shunjiro
S
Kitazawa
Shigeru
S
D000818:Animals; D000918:Antibody Specificity; D001522:Behavior, Animal / Q000502:physiology; D002452:Cell Count; D007600:Judgment / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D013003:Somatosensory Cortex / Q000166:cytology / Q000378:metabolism; D013194:Staining and Labeling; D013997:Time Factors
D016760:Proto-Oncogene Proteins c-fos
Learn Mem. 2000 Mar-Apr;7(2):116-22
10753978
Neuroreport. 1998 Aug 24;9(12):2733-6
9760111
J Neurophysiol. 2005 May;93(5):2856-63
15634708
Eur J Neurosci. 2002 Feb;15(4):735-43
11886453
J Neurosci. 2001 Jul 15;21(14):5251-61
11438600
Naunyn Schmiedebergs Arch Pharmacol. 2003 Nov;368(5):331-41
14574439
J Comp Neurol. 2003 Dec 8;467(2):135-49
14595765
Neuroscience. 1997 Mar;77(1):175-86
9044385
Behav Brain Res. 1991 Aug 29;44(2):133-43
1751004
Brain Res Cogn Brain Res. 1996 Jun;3(3-4):227-42
8806025
Acta Neurobiol Exp (Wars). 2005;65(2):191-4
15960305
Trends Neurosci. 1989 Nov;12(11):459-62
2479148
Nature. 1987 Aug 13-19;328(6131):632-4
3112583
Nat Neurosci. 2005 Jul;8(7):850-1
16136670
Behav Brain Res. 2005 Feb 10;157(1):167-75
15617783
J Neurosci. 2003 Nov 26;23(34):10963-70
14645492
Behav Brain Res. 2010 Mar 17;208(1):158-62
19931567
Nature. 2003 Feb 27;421(6926):911
12606990
Exp Brain Res. 2007 Jun;179(4):709-21
17216148
Neuroreport. 1997 Sep 8;8(13):iii-vii
9376510
Nat Neurosci. 2001 Jul;4(7):759-65
11426234
J Comp Neurol. 2004 Nov 22;479(4):360-73
15514982
J Comp Neurol. 2003 Nov 24;466(4):525-44
14566947
J Neurosci. 1994 Feb;14(2):611-29
8301355
Neurobiol Aging. 1997 Jan-Feb;18(1):45-55
8983032
Behav Brain Res. 2003 Nov 30;146(1-2):65-75
14643460
Nat Neurosci. 2001 Oct;4(10):979-80
11544483
Neuroscience. 2005;135(1):285-97
16084664
Neuropsychologia. 2004;42(14):1887-95
15381018
Behav Brain Res. 2000 Jan;107(1-2):123-32
10628736
Laterality. 1996 Jul;1(2):97-137
15513031
Brain Res Cogn Brain Res. 2002 Jun;14(1):153-63
12063139
Neuroscience. 2008 Jun 2;153(4):1402-17
18440715
Neurosci Res. 2006 Sep;56(1):96-102
16846658
Curr Biol. 2002 Mar 5;12(5):359-68
11882286
Science. 1988 Jun 3;240(4857):1328-31
3131879
PLoS One. 2008 Sep 25;3(9):e3266
18815614
Neuron. 1990 Apr;4(4):477-85
1969743
Neuroscience. 1998 Mar;83(2):501-15
9460758
J Exp Psychol. 1961 Nov;62:423-32
13907740
Curr Opin Neurobiol. 2008 Apr;18(2):131-6
18639634
Eur J Neurosci. 1999 Nov;11(11):4089-98
10583497
Brain Res Mol Brain Res. 1992 Jan;12(1-3):141-7
1312199
Trends Cogn Sci. 2002 Aug 1;6(8):323-325
12140076
Cereb Cortex. 2005 Nov;15(11):1690-6
15703251
J Neurosci Methods. 1989 Sep;29(3):261-5
2507830
J Comp Neurol. 1997 Mar 31;380(1):145-53
9073089
J Comp Neurol. 2005 May 23;486(1):48-60
15834958
Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):2167-72
14766982
Nat Rev Neurosci. 2005 Oct;6(10):755-65
16163383
Hippocampus. 1998;8(5):533-47
9825963
J Neurosci. 2000 Aug 15;20(16):6030-8
10934252