title: Transfused stored platelets have the same haemostatic function as circulating native platelets.
abstract: OBJECTIVE
  As thrombelastography (TEG) measures haemostasis in whole blood, we used this instrument to study whether transfused platelets (PLTs) have the same haemostatic function compared to native circulating PLTs. Further, we studied the effect of storage time on the haemostatic potential of platelet concentrates (PCs).
  
  
  METHODS
  During the decrease in PLT count after chemotherapy, TEG parameters were measured serially until the transfusion trigger was reached in 92 patients. TEG parameters for different ranges of native circulating PLTs could be assessed, which were compared to ranges obtained in the thrombocytopenic period in which the patient received PLT transfusions. Finally, we compared the haemostatic potential of fresh PCs (1-3 days) with PCs with longer storage time (4-5 days).
  
  
  RESULTS
  No differences could be found in haemostatic potential between native PLTs and transfused stored PLTs (all P-values > or = 0.1). The transfusion of fresh PLTs demonstrated better haemostatic effects than longer stored PLTs, measured 1 h after transfusion. Both the time until a fixed level of clot firmness was reached (K-time) and the rate of clot growth (alpha angle) were superior for fresh PCs.
  
  
  CONCLUSIONS
  TEG is able to monitor the haemostatic effects of PLT transfusion, with comparable haemostatic properties of native circulating and transfused stored-PLTs. Further, our data suggest that limited storage time is associated with a better haemostatic capacity. However, before TEG can be applied as a qualitative test in PLT transfusion, further research is needed with focus on clinical outcomes like bleeding episodes.
authors.affiliation: Department of Hematology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
authors.forename: W W H
authors.initials: WW
authors.lastname: Roeloffzen
authors.forename: H C
authors.initials: HC
authors.lastname: Kluin-Nelemans
authors.forename: N J G M
authors.initials: NJ
authors.lastname: Veeger
authors.forename: L
authors.initials: L
authors.lastname: Bosman
authors.forename: J Th M
authors.initials: JT
authors.lastname: de Wolf
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D005260:Female; D006487:Hemostasis; D006801:Humans; D008297:Male; D008875:Middle Aged; D017713:Platelet Transfusion / Q000295:instrumentation / Q000379:methods*; D010983:Plateletpheresis; D013916:Thrombelastography / Q000379:methods; D055815:Young Adult