Feasibility of in vivo 15N MRS detection of hyperpolarized 15N labeled choline in rats.
The increase of total choline in tumors has become an important biomarker in cancer diagnosis. Choline and choline metabolites can be measured in vivo and in vitro using multinuclear MRS. Recent in vivo(13)C MRS studies using labeled substrates enhanced via dynamic nuclear polarization demonstrated the tremendous potential of hyperpolarization for real-time metabolic studies. The present study demonstrates the feasibility of detecting hyperpolarized (15)N labeled choline in vivo in a rat head at 9.4 T. We furthermore report the in vitro (172 +/- 16 s) and in vivo (126 +/- 15 s) longitudinal relaxation times. We conclude that with appropriate infusion protocols it is feasible to detect hyperpolarized (15)N labeled choline in live animals.
Cudalbu
Cristina
C
Laboratory for Functional and Metabolic Imaging, Ecole Polytechnique Fédérale de Lausanne, EPFL-SB-IPMC-LIFMET, Office F1 602, Station 6, CH-1015 Lausanne, Switzerland. cristina.cudalbu@epfl.ch
Comment
Arnaud
A
Kurdzesau
Fiodar
F
van Heeswijk
Ruud B
RB
Uffmann
Kai
K
Jannin
Sami
S
Denisov
Vladimir
V
Kirik
Deniz
D
Gruetter
Rolf
R
D000818:Animals; D002794:Choline / Q000737:chemistry*; D007553:Isotope Labeling; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D009587:Nitrogen Isotopes / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D009587:Nitrogen Isotopes; D002794:Choline