title: Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis.
abstract: Efficacy of IS900 blood PCR was evaluated for the presence of MAP infection. Serum, fecal, and blood samples of kids, young, and adult goats from farm and farmer's herds in Mathura district were also screened by ELISA, microscopy and culture. Of 111 goats (kids: 40, young: 14, adults: 57) screened, 77.5% were positive by blood PCR. Of 76 goats, 90.8% (kids: 87.5% and adults: 94.4%) were positive by PCR. From 21 kids and 14 young goats, 42.8 and 57.1% were positive. gDNA from goats was genotyped as MAP "Indian Bison type". Of 21 fecal samples of kids examined by microscopy, 66.7% were positive. In ELISA, 9.5 and 57.1% kids were positives as "type I" and "type II" reactors, respectively. Screening 14 young goats by culture of blood clots, 28.6% were positive. Agreement was substantial between PCR and microscopy. It was fair and moderate when PCR and microscopy were compared with type I and type II reactors, respectively. Presence of MAP in non-clinical kids and young goats indicate early or subclinical infection. Blood PCR was rapid, sensitive, and specific assay for detection of MAP in any stage (early, subclinical, and clinical) and age (kids, young, and adult) of goats.
authors.affiliation: Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO - Farah, Mathura (UP), Uttar Pradesh 281 122, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Singh
authors.forename: S V
authors.initials: SV
authors.lastname: Singh
authors.forename: H
authors.initials: H
authors.lastname: Kumar
authors.forename: J S
authors.initials: JS
authors.lastname: Sohal
authors.forename: A V
authors.initials: AV
authors.lastname: Singh
references.citation: Vet Microbiol. 1997 Sep;57(2-3):233-44
references.pmid: 9355258
references.citation: Prev Vet Med. 1999 Jun 11;40(3-4):179-92
references.pmid: 10423773
references.citation: J Clin Microbiol. 1992 Jan;30(1):166-71
references.pmid: 1734049
references.citation: Proc Natl Acad Sci U S A. 2005 Aug 30;102(35):12344-9
references.pmid: 16116077
references.citation: Dig Dis Sci. 1986 Dec;31(12):1351-60
references.pmid: 3803136
references.citation: Rev Sci Tech. 2001 Apr;20(1):133-50
references.pmid: 11288509
references.citation: J Vet Diagn Invest. 1992 Jan;4(1):23-7
references.pmid: 1554765
references.citation: Lancet. 2004 Sep 18-24;364(9439):1039-44
references.pmid: 15380962
references.citation: Can J Vet Res. 1991 Jul;55(3):252-9
references.pmid: 1909601
references.citation: J Am Vet Med Assoc. 1989 May 15;194(10):1423-6
references.pmid: 2722634
references.citation: J Am Vet Med Assoc. 1987 Mar 15;190(6):681-3
references.pmid: 3553118
references.citation: Aust Vet J. 2001 Apr;79(4):267-78
references.pmid: 11349414
references.citation: Anal Biochem. 1995 Apr 10;226(2):325-30
references.pmid: 7793635
references.citation: Vet Microbiol. 2006 Jan 10;112(1):33-41
references.pmid: 16300910
references.citation: Vet Immunol Immunopathol. 1998 Dec 11;66(3-4):343-58
references.pmid: 9880110
references.citation: Aust Vet J. 1999 Feb;77(2):113-9
references.pmid: 10078359
references.citation: Rev Sci Tech. 2005 Dec;24(3):1061-6
references.pmid: 16642774
references.citation: J Comp Pathol. 1997 Apr;116(3):217-61
references.pmid: 9147244
references.citation: J Vet Diagn Invest. 1995 Jul;7(3):400-1
references.pmid: 7578461
references.citation: FEMS Microbiol Lett. 2001 Mar 1;196(1):31-7
references.pmid: 11257544
references.citation: Infect Immun. 1987 Jul;55(7):1588-93
references.pmid: 3596802
references.citation: Am J Epidemiol. 1987 Aug;126(2):161-9
references.pmid: 3300279
references.citation: J Comp Pathol. 1996 May;114(4):419-37
references.pmid: 8814536
references.citation: FEMS Microbiol Lett. 2004 Aug 1;237(1):119-26
references.pmid: 15268946
references.citation: Clin Diagn Lab Immunol. 2002 Nov;9(6):1367-71
references.pmid: 12414776
references.citation: Clin Microbiol Rev. 2001 Jul;14(3):489-512
references.pmid: 11432810
references.citation: J Vet Diagn Invest. 2005 Jul;17(4):354-9
references.pmid: 16130994
references.citation: Comp Immunol Microbiol Infect Dis. 2008 Sep;31(5):419-33
references.pmid: 17854892
references.citation: Vet Rec. 1993 Dec 11;133(24):599-600
references.pmid: 8116172
references.citation: Indian J Microbiol. 2007 Sep;47(3):251-8
references.pmid: 23100673
references.citation: FEMS Microbiol Lett. 2002 Apr 9;209(2):267-71
references.pmid: 12007816
references.citation: Mol Cell Probes. 2001 Jun;15(3):139-45
references.pmid: 11352594
references.citation: Aust Vet J. 2000 Aug;78(8):560-6
references.pmid: 10979513
references.citation: Immunol Cell Biol. 1999 Aug;77(4):364-72
references.pmid: 10457205
references.citation: Vet Rec. 2005 Jun 25;156(26):825-31
references.pmid: 15980134
references.citation: J Vet Diagn Invest. 2003 Mar;15(2):195-200
references.pmid: 12661735
references.citation: J Vet Diagn Invest. 2005 Jul;17(4):350-4
references.pmid: 16130993
references.citation: Aust Vet J. 2004 Aug;82(8):497-503
references.pmid: 15359966
references.citation: Biometrics. 1977 Mar;33(1):159-74
references.pmid: 843571
references.citation: J Clin Microbiol. 2000 Aug;38(8):3048-54
references.pmid: 10921976
references.citation: J Biol Chem. 1951 Nov;193(1):265-75
references.pmid: 14907713