Immunohistochemical localization of AMPA-type glutamate receptor subunits in the striatum of rhesus monkey.
Corticostriatal and thalamostriatal projections utilize glutamate as their neurotransmitter. Their influence on striatum is mediated, in part, by ionotropic AMPA-type glutamate receptors, which are heteromers composed of GluR1-4 subunits. While the cellular localization of AMPA-type subunits in the basal ganglia has been well characterized in rodents, the cellular localization of AMPA subunits in primate basal ganglia is not. We thus carried out immunohistochemical studies of GluR1-4 distribution in rhesus monkey basal ganglia in conjunction with characterization of each major neuron type. In striatum, about 65% of striatal neurons immunolabeled for GluR1, 75%-79% immunolabeled for GluR2 or GluR2/3, and only 2.5% possessed GluR4. All neurons the large size of cholinergic interneurons (mean diameter 26.1 microm) were moderately labeled for GluR1, while all neurons in the size range of parvalbuminergic interneurons (mean diameter 13.8 microm) were intensely rich in GluR1. Additionally, somewhat more than half of the neurons in the size range of projection neurons (mean diameter 11.6 microm) immunolabeled for GluR1, and about one third of these were very rich in GluR1. About half of the neurons the size of cholinergic interneurons were immunolabeled for GluR2, and the remainder of the neurons that were immunolabeled for GluR2 coincided with projection neurons in size and shape (GluR2 diameter=10.7 microm), indicating that the vast majority of striatal projection neurons possess immunodectible GluR2. Similar results were observed with GluR2/3 immunolabeling. Half of the neurons the size of cholinergic interneurons immunolabeled for GluR4 and seemingly all neurons in the size range of parvalbuminergic interneurons possessed GluR4. These results indicate that AMPA receptor subunit combinations for striatal projection neurons in rhesus monkey are similar to those for the corresponding neuron types in rodents, and thus their AMPA responses to glutamate are likely to be similar to those demonstrated in rodents.
Deng
Yun-Ping
YP
Department of Anatomy & Neurobiology, University of Tennessee Health Science Center, 855 Monroe Avenue, Memphis, TN 38163, USA. ydeng@uthsc.edu
Shelby
Evan
E
Reiner
Anton J
AJ
D000818:Animals; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism*; D008253:Macaca mulatta / Q000033:anatomy & histology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000145:classification / Q000378:metabolism*; D021122:Protein Subunits / Q000378:metabolism*; D018091:Receptors, AMPA / Q000145:classification / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D021122:Protein Subunits; D018091:Receptors, AMPA
Brain Res. 1993 May 7;610(2):305-10
8100472
Brain Res Mol Brain Res. 1997 Jun;46(1-2):281-9
9191103
Exp Neurol. 1994 Sep;129(1):37-56
7925841
Exp Neurol. 1999 Sep;159(1):283-96
10486197
Neuroscience. 2009 Jan 12;158(1):45-54
18304745
Neuroscience. 1998 Apr;83(3):749-61
9483559
Development. 1992 Sep;116(1):201-11
1483388
Neuroscience. 1994 Aug;61(3):509-19
7969927
Cell Calcium. 2002 Jan;31(1):13-25
11990296
J Neurosci. 2002 Mar 1;22(5):1967-75
11880527
J Neurosci. 2001 Apr 1;21(7):2224-39
11264298
J Gerontol. 1974 Jul;29(4):366-81
4209545
J Comp Neurol. 1996 Jan 01;364(1):5
8789271
J Neurosci. 1993 Feb;13(2):782-92
7678861
Nat Neurosci. 1999 Jan;2(1):57-64
10195181
J Chem Neuroanat. 2004 Jun;27(3):143-64
15183201
Neurosci Lett. 1989 May 22;100(1-3):99-104
2761790
Trends Neurosci. 1990 Jul;13(7):286-9
1695405
J Neurochem. 2000 Feb;74(2):839-46
10646537
J Comp Neurol. 1992 Apr 15;318(3):329-54
1374769
Science. 1994 Dec 9;266(5191):1709-13
7992055
J Comp Neurol. 2004 Feb 9;469(3):325-39
14730585
J Neurosci. 1991 Jun;11(6):1649-59
1710657
Brain Res. 1993 Apr 23;609(1-2):137-48
8508297
J Histochem Cytochem. 1999 Jun;47(6):719-30
10330448
Anat Rec. 1946 Feb;94:239-47
21015608
J Neuropathol Exp Neurol. 1980 Sep;39(5):598-605
7218001
Nat Neurosci. 1999 May;2(5):467-72
10321252
Neuroscience. 1996 Aug;73(3):715-31
8809793
Exp Neurol. 1996 Dec;142(2):296-312
8934561
Eur J Neurosci. 2000 Aug;12(8):2871-80
10971630
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8780-4
3909155
Neurosci Lett. 1994 Nov 21;182(1):51-4
7891886
Trends Neurosci. 1997 Oct;20(10):464-70
9347614
Trends Neurosci. 1995 Dec;18(12):527-35
8638293
J Chem Neuroanat. 2007 Jul;33(4):167-92
17446041
Eur J Neurosci. 1996 Jan;8(1):229-33
8713467
Neuroreport. 1993 Dec 13;5(3):205-8
7507722
J Neurosci. 1997 Apr 1;17(7):2477-91
9065508
Brain Res. 1996 Aug 19;730(1-2):232-7
8883909
J Chem Neuroanat. 2001 Jul;22(1-2):13-42
11470552
Exp Neurol. 1997 Aug;146(2):444-57
9270055
Trends Neurosci. 1993 Sep;16(9):359-65
7694406
Brain Res. 1997 Dec 5;778(1):43-55
9462876
Life Sci. 1984 Jul 2;35(1):19-32
6234446
J Comp Neurol. 1987 Jan 8;255(2):245-58
3819015
J Neurosci. 1997 Mar 1;17(5):1570-81
9030617
Cell Calcium. 1990 Oct;11(9):599-602
2285928
Brain Res. 1990 Dec 17;536(1-2):1-15
2085740
J Neurosci. 2008 Jun 18;28(25):6483-92
18562619
Acta Neurobiol Exp (Wars). 1996;56(1):313-22
8787192
Annu Rev Neurosci. 1992;15:285-320
1575444
Brain Res Mol Brain Res. 1997 Dec 15;52(2):235-41
9495544
J Neurosci Methods. 1988 Nov;26(1):1-23
3199846
Neuron. 1995 Nov;15(5):987-90
7576666
Trends Neurosci. 2004 Sep;27(9):520-7
15331233
J Comp Neurol. 1983 Jul 1;217(3):264-70
6136532
Neurosci Res. 1996 Aug;25(4):309-34
8866512
Ann Neurol. 1990 Jun;27(6):620-5
1972876
Nature. 1986 May 8-14;321(6066):168-71
2422561
Exp Neurol. 1993 Jan;119(1):46-71
8432351
Science. 2006 Mar 3;311(5765):1253-6
16513974
Brain Res. 1996 Feb 12;709(1):145-50
8869567
Science. 1990 Aug 3;249(4968):556-60
2166337
J Comp Neurol. 1994 Sep 15;347(3):340-56
7822489
Mol Cell Neurosci. 2001 Jun;17(6):1025-33
11414791
Lancet. 1988 Jun 4;1(8597):1252-5
2897519
Brain Res Bull. 1987 Mar;18(3):447-55
3580914
Nature. 2007 Feb 8;445(7128):643-7
17287809
J Neurosci Methods. 1994 Oct;54(2):219-37
7869754
Exp Neurol. 1990 Nov;110(2):209-18
1977609
Neuroscience. 1987 Feb;20(2):577-87
2438593
Crit Rev Neurobiol. 1996;10(2):239-63
8971131
Annu Rev Physiol. 2004;66:161-81
14977400
Neurobiol Aging. 2000 Jul-Aug;21(4):599-606
10924778
J Neurosci. 1995 Nov;15(11):7046-61
7472460
Neuron. 1995 Jul;15(1):193-204
7619522
Neuroscience. 2009 Jan 12;158(1):105-25
18424006
Brain Res Brain Res Rev. 2000 Nov;34(1-2):80-101
11086188
Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10499-503
1438239
Neuroscience. 1993 Mar;53(2):327-58
8388083
J Neurosci. 1997 Jan 15;17(2):819-33
8987803
J Comp Neurol. 1981 Feb 1;195(4):567-84
6161949
Prog Brain Res. 1993;99:51-72
8108557
Brain Res. 1995 Nov 20;699(2):297-304
8616634
J Neurosci. 2000 Jan 1;20(1):123-32
10627588
Brain Res. 2000 Apr 28;863(1-2):182-91
10773206
J Neurosci. 1997 Dec 15;17(24):9393-406
9390995
Exp Neurol. 1998 Feb;149(2):356-72
9500958
J Neurosci. 2000 Jun 15;20(12):4480-8
10844017
Neurosci Res. 2008 Dec;62(4):216-24
18801393
Brain Res. 1992 May 15;580(1-2):215-24
1504801
Annu Rev Neurosci. 1994;17:31-108
8210177
J Neurosci. 1993 Nov;13(11):4908-23
7693897
Ann Neurol. 1998 May;43(5):586-97
9585352
J Neurol Sci. 2000 Nov 1;180(1-2):29-34
11090861
Neuron. 2004 Oct 28;44(3):483-93
15504328
Prog Neurobiol. 2007 Apr;81(5-6):272-93
17188796
J Comp Neurol. 1996 May 13;368(4):553-68
8744443
Brain Res. 1994 Jan 7;633(1-2):331-6
8137167
Brain Dev. 1997 Sep;19(6):388-92
9339865
Neuroscience. 1999;93(1):89-97
10430473
J Biol Chem. 1992 Jan 5;267(1):501-7
1309749
J Neurosci. 1985 Mar;5(3):776-94
2983048
Exp Neurol. 1995 Dec;136(2):212-24
7498411
J Neurosci. 2008 Oct 22;28(43):10814-24
18945889
Brain Res. 1996 Oct 7;735(2):317-24
8911672
Neuroscience. 1999;88(3):775-93
10363817
J Neurosci. 1997 Jan 1;17(1):204-15
8987749
Neuroscience. 1992 Dec;51(3):533-45
1488113
J Comp Neurol. 1998 Jan 26;390(4):552-63
9450535
J Neurosci. 2004 Sep 22;24(38):8289-99
15385612
Brain Res. 1991 Dec 24;568(1-2):235-43
1839966
Eur J Neurosci. 1991;3(12):1316-1329
12106229
J Biol Chem. 2003 Aug 29;278(35):33364-9
12810713
Dev Neurosci. 1998;20(2-3):242-52
9691198
Exp Neurol. 2000 Dec;166(2):307-23
11085896
J Comp Neurol. 2003 Mar 17;457(4):420-40
12561080
Brain Res. 1986 May 7;372(2):241-52
2871900