Improvement and optimization of a multiplex real-time reverse transcription polymerase chain reaction assay for the detection and typing of Vesicular stomatitis virus.
An improvement to a previously reported real-time reverse transcription polymerase chain reaction (real-time RT-PCR) assay for the detection of Vesicular stomatitis virus (VSV) is described. Results indicate that the new assay is capable of detecting a panel of genetically representative strains of VSV present in North, Central, and South America. The assay is specific for VSV and allows for simultaneous differentiation between Vesicular stomatitis Indiana virus and Vesicular stomatitis New Jersey virus. This real-time RT-PCR is able to detect current circulating strains of VSV and can be used for rapid diagnosis of VSV and differentiation of VSV from other vesicular diseases, such as foot-and-mouth disease.
Hole
Kate
K
National Centre for Foreign Animal Disease, 1015 Arlington St, Winnipeg MB R3E 3M4, Canada.
Velazquez-Salinas
Lauro
L
Velazques-Salinas
Lauro
L
Clavijo
Alfonso
A
D000818:Animals; D020137:Base Pair Mismatch; D001483:Base Sequence; D002489:Central America / Q000453:epidemiology; D017931:DNA Primers; D015342:DNA Probes; D005784:Gene Amplification; D008800:Mexico / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012689:Sequence Homology, Nucleic Acid; D013020:South America / Q000453:epidemiology; D014481:United States / Q000453:epidemiology; D054243:Vesicular Stomatitis / Q000453:epidemiology*; D014721:Vesicular stomatitis Indiana virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D054260:Vesicular stomatitis New Jersey virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018116:Vesiculovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers; D015342:DNA Probes