title: Wiring of pyranose dehydrogenase with osmium polymers of different redox potentials.
abstract: In this study, five different flexible osmium based redox polymers were investigated for their ability to efficiently "wire" the oxidoreductase pyranose dehydrogenase (PDH, EC 1.1.99.29) from Agaricus meleagris, on graphite electrodes for possible applications in biofuel cells. A series of newly synthesised osmium based redox polymers covering the potential range between -270 and +160 mV vs. Ag|AgCl (0.1M KCl) was used. The performance of the redox polymers for enzyme wiring was investigated using glucose as substrate. The optimal operational conditions such as pH and potential were investigated.
authors.affiliation: Department of Analytical Chemistry, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
authors.forename: Muhammad Nadeem
authors.initials: MN
authors.lastname: Zafar
authors.forename: Federico
authors.initials: F
authors.lastname: Tasca
authors.forename: Susan
authors.initials: S
authors.lastname: Boland
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kujawa
authors.forename: Ilabahen
authors.initials: I
authors.lastname: Patel
authors.forename: Clemens K
authors.initials: CK
authors.lastname: Peterbauer
authors.forename: Dónal
authors.initials: D
authors.lastname: Leech
authors.forename: Lo
authors.initials: L
authors.lastname: Gorton
mesh_terms: D000364:Agaricus / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009942:Organometallic Compounds / Q000737:chemistry*; D009992:Osmium / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*; D012834:Silver / Q000737:chemistry; D018030:Silver Compounds / Q000737:chemistry
chemical_list: D009942:Organometallic Compounds; D011108:Polymers; D018030:Silver Compounds; D009992:Osmium; D012834:Silver; D006108:Graphite; D002237:Carbohydrate Dehydrogenases; D005947:Glucose; C037548:silver chloride