title: Crystallization and preliminary crystallographic studies of a flavin-dependent thymidylate synthase from Helicobacter pylori.
abstract: The ThyX enzymes that have recently been identified in various bacteria, including some important human pathogens such as Helicobacter pylori and Mycobacterium tuberculosis, are flavin-dependent thymidylate synthases that function in the place of classic thymidylate synthase enzymes in the biosynthesis of dTMP, which is one of the building blocks of DNA. They are promising targets for the development of novel antibiotics because they utilize catalytic mechanisms that are distinct from those of the classic thymidylate synthases found in most organisms, including humans. In this study, H. pylori ThyX was purified and crystallized in complex with flavin adenine dinucleotide (FAD) and a diffraction data set was collected to 2.5 A resolution. The crystals belonged to space group C2, with unit-cell parameters a = 221.92, b = 49.43, c = 143.02 A, beta = 98.84 degrees .
authors.affiliation: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, People's Republic of China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.forename: Jinyong
authors.initials: J
authors.lastname: Zhang
authors.forename: Xuhu
authors.initials: X
authors.lastname: Mao
authors.forename: Quanming
authors.initials: Q
authors.lastname: Zou
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Hu
authors.forename: Da Cheng
authors.initials: da C
authors.lastname: Wang
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D005415:Flavins / Q000378:metabolism; D016480:Helicobacter pylori / Q000201:enzymology*; D013940:Thymidylate Synthase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005415:Flavins; D013940:Thymidylate Synthase
references.citation: Annu Rev Biochem. 1995;64:721-62
references.pmid: 7574499
references.citation: Structure. 2003 Jun;11(6):677-90
references.pmid: 12791256
references.citation: Biochemistry. 2004 Aug 17;43(32):10295-301
references.pmid: 15301527
references.citation: Science. 2002 Jul 5;297(5578):105-7
references.pmid: 12029065
references.citation: Biochem Soc Trans. 2004 Apr;32(Pt 2):231-5
references.pmid: 15046578
references.citation: Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):48-57
references.pmid: 16369093
references.citation: Nature. 2009 Apr 16;458(7240):919-23
references.pmid: 19370033
references.citation: J Biol Chem. 2006 Aug 18;281(33):24048-57
references.pmid: 16707489