title: Isolation and partial sequencing of Equid herpesvirus 5 from a horse in Iceland.
abstract: Horses are hosts to 2 types of gammaherpesviruses, Equid herpesvirus 2 and 5 (EHV-2 and EHV-5, respectively). Both EHV-2 and EHV-5 are common in horses in Iceland. An Icelandic EHV-5 isolate was recovered by sequential culture in primary fetal horse kidney and rabbit kidney cells. Glycoprotein B, glycoprotein H, and DNA terminase genes of the isolate were fully sequenced, and the DNA polymerase gene was partly sequenced. To date, the glycoprotein B gene of EHV-5 was the only gene that has been reported to be completely sequenced in addition to small parts of the glycoprotein H, DNA polymerase, and DNA terminase genes. The present report, therefore, is a significant addition to previously reported EHV-5 sequences.
authors.affiliation: Institute for Experimental Pathology University of Iceland, Keldur v/Vesturlandsveg, IS-112 Reykjavík, Iceland.
authors.forename: Lilja
authors.initials: L
authors.lastname: Thorsteinsdóttir
authors.forename: Einar G
authors.initials: EG
authors.lastname: Torfason
authors.forename: Sigurbjörg
authors.initials: S
authors.lastname: Torsteinsdóttir
authors.forename: Vilhjálmur
authors.initials: V
authors.lastname: Svansson
mesh_terms: D000818:Animals; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005260:Female; D006023:Glycoproteins / Q000235:genetics; D006566:Herpesviridae Infections / Q000235:genetics / Q000662:veterinary*; D006734:Horse Diseases / Q000382:microbiology / Q000821:virology*; D006736:Horses / Q000821:virology*; D007055:Iceland; D007668:Kidney / Q000821:virology; D008089:Listeria monocytogenes; D008088:Listeriosis / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits; D018144:Rhadinovirus / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D018140:Varicellovirus / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000235:genetics; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics
chemical_list: D006023:Glycoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C121062:glycoprotein B, Equid herpesvirus 5; D004259:DNA-Directed DNA Polymerase