Transferability, amplification quality, and genome specificity of microsatellites in Brassica carinata and related species.
No information is available on the transferability and amplification quality of microsatellite (SSR) markers of the public domain in Brassica carinata A. Braun. The objective of the presented research was to study the amplification of a set of 73 SSRs from B. nigra (L.) Koch and B. napus L. in B. carinata, and to compare the results with those obtained in the amplification of the same markers in other Brassica species of the U triangle. This set of SSRs from B. nigra (B genome) and B. napus (AC genome) allows the identification of the 3 basic genomes of the Brassica species tested. 94.3% of the SSR markers from B. nigra and 97.4% of those from B. napus amplified SSR-specific products in B. carinata. Very high-quality amplification with a strong signal and easy scoring in B. carinata was recorded for 52.8% of the specific loci from B. nigra SSRs and 59.3% of the specific loci from B. napus SSRs, compared to 66.7% in B. nigra and 62.8% in B. napus. Genome specificity and amplification quality of B. nigra and B. napus SSR markers in the 6 species under study is reported. High-quality transferable SSR markers provide an efficient and cost-effective platform to advance in molecular research in B. carinata.
Marquez-Lema
A
A
Institute for Sustainable Agriculture (CSIC), Córdoba, Spain.
Velasco
L
L
Perez-Vich
B
B
D001937:Brassica / Q000235:genetics*; D029688:Brassica napus / Q000235:genetics; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D005784:Gene Amplification / Q000235:genetics*; D018014:Gene Transfer Techniques; D018745:Genome, Plant; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods
D018744:DNA, Plant
Hereditas. 2004;140(3):245-8
15198715
Theor Appl Genet. 2005 May;110(7):1210-7
15806349
Theor Appl Genet. 1992 Nov;85(2-3):331-40
24197323
Cancer Res. 1967 Feb;27(2):209-20
6018555
Theor Appl Genet. 2004 Apr;108(6):1103-12
15067397
Theor Appl Genet. 1995 Jul;91(2):195-9
24169763
Theor Appl Genet. 1991 Jul;82(1):81-92
24212864