title: Glucose parameters are altered in mouse offspring produced by assisted reproductive technologies and somatic cell nuclear transfer.
abstract: Fortunately, the majority of children conceived through assisted reproductive technologies (ARTs) appear healthy; however, metabolic abnormalities, including elevated glucose and increased and altered adipose tissue deposition, have been reported in adolescents. To parse out factors that may be responsible, we investigated the effects of two different ARTs--in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI)--as well as somatic cell nuclear transfer (SCNT) on glucose clearance, body weight, and body composition of young adult mice. Female and male mice generated through ART weighed more than control (naturally conceived [STOCK]) mice at birth. No differences in body weight were observed in males up to 8 wk of age. ART females took longer than control mice to clear a glucose bolus, with glucose clearance most impaired in SCNT females. IVF females secreted more insulin and had a higher insulin peak 15 min after glucose injection compared with all other groups. Male mice exhibited no differences in glucose clearance, but IVF males required more insulin to do so. SCNT females weighed more than IVF, ICSI, and STOCK females, and they had higher fat content than ICSI females and higher leptin levels than all other groups. These results show that glucose parameters are altered in young adult mice conceived through techniques associated with ART before onset of obesity and may be responsible for its development later in life. The present study suggests that more investigation regarding the long-term effects of manipulations associated with ART is warranted.
authors.affiliation: Department of Psychiatry, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA. scottk2@mail.uc.edu
authors.forename: Karen A
authors.initials: KA
authors.lastname: Scott
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Miyuki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Yanling
authors.initials: Y
authors.lastname: Lin
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Melhorn
authors.forename: Eric G
authors.initials: EG
authors.lastname: Krause
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Woods
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Yanagimachi
authors.forename: Randall R
authors.initials: RR
authors.lastname: Sakai
authors.forename: Kellie L K
authors.initials: KL
authors.lastname: Tamashiro
mesh_terms: D050154:Adiposity; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001823:Body Composition; D001835:Body Weight; D005260:Female; D005307:Fertilization in Vitro / Q000009:adverse effects; D005947:Glucose / Q000378:metabolism*; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008810:Mice, Inbred C57BL; D053652:Nuclear Transfer Techniques / Q000009:adverse effects*; D009765:Obesity / Q000209:etiology; D011247:Pregnancy; D027724:Reproductive Techniques, Assisted / Q000009:adverse effects*; D012737:Sex Factors; D020554:Sperm Injections, Intracytoplasmic / Q000009:adverse effects
chemical_list: D001786:Blood Glucose; D007328:Insulin; D020738:Leptin; D005947:Glucose
references.citation: J Clin Endocrinol Metab. 2007 Sep;92(9):3417-23
references.pmid: 17595253
references.citation: Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1595-600
references.pmid: 14747652
references.citation: Reprod Biomed Online. 2005 Feb;10(2):247-88
references.pmid: 15823233
references.citation: BMC Dev Biol. 2007 Oct 18;7:116
references.pmid: 17949482
references.citation: Hum Reprod. 2009 Nov;24(11):2788-95
references.pmid: 19648588
references.citation: Biol Reprod. 2008 Apr;78(4):761-72
references.pmid: 18199884
references.citation: Fertil Steril. 2008 Nov;90(5):1662-73
references.pmid: 18163998
references.citation: Dev Neurosci. 2009;31(4):285-92
references.pmid: 19546565
references.citation: Mol Cell Endocrinol. 1998 May 25;140(1-2):115-20
references.pmid: 9722178
references.citation: Stem Cells. 2007 May;25(5):1279-85
references.pmid: 17255518
references.citation: Hum Reprod. 2000 Dec;15 Suppl 5:68-86
references.pmid: 11263539
references.citation: Biol Reprod. 1995 Oct;53(4):855-62
references.pmid: 8547481
references.citation: Zygote. 2001 Nov;9(4):277-82
references.pmid: 11771892
references.citation: Biol Reprod. 2003 Oct;69(4):1394-400
references.pmid: 12801984
references.citation: Horm Metab Res. 2002 Nov-Dec;34(11-12):616-21
references.pmid: 12660870
references.citation: Hum Fertil (Camb). 2000;3(4):238-246
references.pmid: 11844385
references.citation: Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):5880-5
references.pmid: 15079084
references.citation: Reprod Fertil Dev. 1991;3(5):501-17
references.pmid: 1788391
references.citation: Nat Med. 2002 Mar;8(3):262-7
references.pmid: 11875497
references.citation: Biol Reprod. 2000 Jul;63(1):328-34
references.pmid: 10859275
references.citation: Biochem Biophys Res Commun. 2006 Feb 3;340(1):183-9
references.pmid: 16356478
references.citation: Exp Biol Med (Maywood). 2003 Nov;228(10):1193-200
references.pmid: 14610260
references.citation: Reprod Domest Anim. 2009 Aug;44(4):634-7
references.pmid: 19019063
references.citation: BJOG. 2005 Dec;112(12):1589-94
references.pmid: 16305560
references.citation: Mt Sinai J Med. 2009 Dec;76(6):506-20
references.pmid: 20014419
references.citation: Biol Reprod. 2008 Feb;78(2):299-306
references.pmid: 17989357
references.citation: Rev Reprod. 1998 Sep;3(3):155-63
references.pmid: 9829550
references.citation: J Clin Endocrinol Metab. 2008 May;93(5):1682-8
references.pmid: 18285409
references.citation: Nature. 1998 Jul 23;394(6691):369-74
references.pmid: 9690471
references.citation: Gend Med. 2009;6 Suppl 1:60-75
references.pmid: 19318219