[Anti-adenovirus activity of a substance and medical form of ribamydil in cell culture].
The inhibiting effect of ribamydil on adenovirus reproduction was studied under the determination of the number of cells with virus- induced DNA-containing intranucleus inclusion bodies and hexone antigen, the synthesis of adenovirus proteins and the infection virus by t he investigation. EC50 of ribamydil substance is 4-8 microg/ml, but complete suppression of adenovirus genome expression was found when adding ribamydil after the virus adsorption, in concentrations of 125-500 microg/ml. The original effect of ribamydil on the expression of adenovirus genome was found under its effect in concentration of 31 microg/ml. Intranucleus virus-induced inclusion bodies of the early type only were found under these conditions. Synthesis of the structural virus polypeptides, including hexone polypeptide (II) and non-structural polypeptide 100K, taking part in hexone trimerization, proceed intensively but without formation of immunologically active hexone. The inhibiting effect of officinal form of ribamydil was less expressed as compared with the substance (EC50: 62 microg/ml). The work results prove that the therapeutic effect of ribamydil (ribavirin) under treatment of adenovirus infections may be achieved in case when it is used in a dose excluding the expression of the adenovirus genome.
Nosach
L N
LN
Diachenko
N S
NS
Zhovnovataia
V L
VL
D000260:Adenoviruses, Human / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D000956:Antigens, Viral / Q000096:biosynthesis; D000998:Antiviral Agents / Q000494:pharmacology*; D036022:Capsid Proteins / Q000096:biosynthesis; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology; D004279:DNA, Viral / Q000096:biosynthesis; D004847:Epithelial Cells / Q000821:virology*; D006367:HeLa Cells; D006801:Humans; D012254:Ribavirin / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
D000956:Antigens, Viral; D000998:Antiviral Agents; D036022:Capsid Proteins; D004279:DNA, Viral; C018315:hexon capsid protein, Adenovirus; D012254:Ribavirin