[Establishment of insulin-resistant muscle cell model induced by tumor necrosis factor-alpha].
OBJECTIVE
To establish a convenient and reliable insulin-resistant cell model induced by TNF-alpha for studying the mechanisms of insulin-resistance and screening medical drugs or functional foods assisted in improving insulin-resistance.


METHODS
L6 cells in TNF-alpha group, insulin group and TNF-alpha plus insulin group were improving incubated in culture medium containing 10 ng/ml TNF-alpha, 100 nmol/L insulin and both 10 ng/ml TNF-alpha and 100 nmol/L insulin respectively for 24 hours. The glucose remained in culture medium was measured by the method of glucose oxidizes peroxides (GOD-POD). The glucose uptake of L6 cells was measured by [3H]-2-deoxyglucose.


RESULTS
With no insulin stimulation, the glucose remained in culture medium in TNF-alpha group was equal to that in the control group (P > 0.05). With insulin stimulation, the glucose remained in culture medium in insulin group was much lower than that in TNF-alpha group (P < 0.05).


CONCLUSIONS
The insulin resistance in L6 cells could be induced by high concentration of TNF-alpha. The IR model of skeletal muscle cell could be widely used for studying the mechanism of insulin resistance and for screening medical drugs and functional food assisted in reducing blood sugar.
Zhang
Zhaofeng
Z
Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.
Zhao
Ming
M
Wang
Junbo
J
Dai
Xiaoqian
X
Ding
Ye
Y
Li
Yong
Y
D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D007333:Insulin Resistance / Q000502:physiology*; D032342:Muscle Cells / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha