title: [Phosphorylation of ATM/ATR substrates in eukaryotic cells after infection with Helicobacter pylori].
abstract: Infection with Helicobacter pylori has been associated with the development of gastric adenocarcinoma in humans, but influence on genetic material of the host cell is still unknown. Here we characterize phosphorylation of ATM-kinase substrates in HeLa and AGS cells in response of Helicobacter pylori attack. Infection with wild-type (cag PAI-positive) and corresponding isogenic cag PAI negative mutant induces activation of Chk1 and Chk2 kinases. Only Chk1 is activated directly by ATM-kinase. We identified group of proteins which are activated by the Helicobacter pylori in AGS cells, using MALDI analyze and two-dimentional gel electrophoresis.
mesh_terms: D064007:Ataxia Telangiectasia Mutated Proteins; D018797:Cell Cycle Proteins / Q000378:metabolism*; D000071877:Checkpoint Kinase 1; D064447:Checkpoint Kinase 2; D004268:DNA-Binding Proteins / Q000378:metabolism; D004789:Enzyme Activation; D006367:HeLa Cells; D016481:Helicobacter Infections / Q000201:enzymology* / Q000235:genetics; D016480:Helicobacter pylori; D006801:Humans; D009154:Mutation; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D025521:Tumor Suppressor Proteins; D011494:Protein Kinases; D064447:Checkpoint Kinase 2; C576324:ATM protein, human; C576597:ATR protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; C000605867:CHEK1 protein, human; C578634:CHEK2 protein, human; D000071877:Checkpoint Kinase 1; D017346:Protein Serine-Threonine Kinases