title: Activation of NMDA receptors is necessary for the recovery of cortical binocularity.
abstract: Classic experiments have indicated that monocular deprivation (MD) for a few days during a critical period of development results in a decrease in the strength of connections mediating responses to the deprived eye, leading to a dramatic breakdown of cortical neuron binocularity. Despite the substantial functional change in the visual cortex, recovery from the effects of MD can be obtained if binocular vision is promptly restored. While great efforts have been made to elucidate the mechanisms regulating loss of deprived eye function, the mechanisms that underlie the recovery of cortical binocularity are poorly understood. Here, we examined whether activation of the N-methyl-d-aspartate receptor (NMDAR) is required for the recovery of cortical binocularity by pharmacologically blocking the NMDAR using d,l-2-amino-5-phosphonopentanoic (APV). Ferrets (n = 10) were monocularly deprived for 6 days, and osmotic minipumps, filled with APV (5.6 mg/ml) or saline, were surgically implanted into the primary visual cortex. One day after surgery, the deprived eye was reopened, and the animals were allowed 24 h of binocular vision. Extracellular recordings showed that intracortical infusion of the NMDAR antagonist, APV, prevented recovery of cortical binocularity while preserving neuronal responsiveness. These findings provide an important new insight for a specific role of NMDARs in the recovery of cortical binocularity from the effects of MD.
authors.affiliation: Department of Anatomy and Neurobiology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298-0709, USA. tekrahe@vcu.edu
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Krahe
authors.forename: Alexandre E
authors.initials: AE
authors.lastname: Medina
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002404:Catheterization; D002452:Cell Count; D002491:Central Nervous System Agents / Q000008:administration & dosage / Q000494:pharmacology; D005289:Ferrets; D008839:Microelectrodes; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010784:Photomicrography; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012683:Sensory Deprivation / Q000502:physiology*; D014633:Valine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D015348:Vision, Binocular / Q000502:physiology*; D015349:Vision, Monocular / Q000502:physiology; D014793:Visual Cortex / Q000187:drug effects / Q000502:physiology*; D014796:Visual Perception / Q000187:drug effects / Q000502:physiology*
chemical_list: D002491:Central Nervous System Agents; D016194:Receptors, N-Methyl-D-Aspartate; C056639:2-amino-5-phosphopentanoic acid; D014633:Valine
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