Kinetic bacteriochlorophyll fluorometer.
A pump and probe fluorometer with a laser diode as single light source has been constructed for measurement of fast induction and relaxation of the fluorescence yield in intact cells, chromatophores and isolated reaction centers of photosynthetic bacteria. The time resolution of the fluorometer is limited by the repetition time of the probing flashes to 20 micros. The apparatus offers high sensitivity, excellent performance and can become a versatile device for a range of demanding applications. Some of them are demonstrated here including fast and easy investigation of the (1) organization and redox state of the photosynthetic apparatus of the intact cells of different bacterial strains and mutants and (2) electron transfer reactions on donor and acceptor sides of isolated reaction centers. The compact design of the mechanics, optics, electronics, and data processing makes the device easy to use as outdoor instrument or to integrate into larger measuring systems.
Kocsis
Péter
P
Department of Experimental Physics, University of Szeged, Rerrich Béla tér 1, Szeged 6720, Hungary.
Asztalos
Emese
E
Gingl
Zoltán
Z
Maróti
Péter
P
D001419:Bacteria / Q000378:metabolism; D001429:Bacteriochlorophylls / Q000378:metabolism*; D005470:Fluorometry / Q000295:instrumentation* / Q000379:methods*; D010788:Photosynthesis / Q000502:physiology
D001429:Bacteriochlorophylls
Photosynth Res. 2007 Jul-Sep;93(1-3):193-203
17487568
Photosynth Res. 2008 Apr;96(1):83-97
18197465
Biochemistry. 2009 Jun 9;48(22):4753-61
19397367
Photosynth Res. 1990 Dec;26(3):181-93
24420583
Biochim Biophys Acta. 2005 Feb 17;1706(3):220-31
15694350
FEBS Lett. 2008 Oct 29;582(25-26):3657-62
18840436
J Phys Chem B. 2007 Apr 26;111(16):4261-70
17394306
J Gen Microbiol. 1962 Sep;28:607-16
13913485
Biochim Biophys Acta. 1999 May 26;1412(1):1-28
10354490
Photosynth Res. 1996 Jan;47(1):41-9
24301706
Nature. 1963 Jan 26;197:355-7
13998015
Nature. 2000 Sep 14;407(6801):177-9
11001053
Photosynth Res. 2006 Jun;88(3):351-6
16763876
Biophys J. 2000 Jul;79(1):14-25
10866934
EMBO J. 1986 Oct;5(10):2445-51
16453713
Biochemistry. 1994 Aug 9;33(31):9237-44
8049225
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3491-5
1081231
Eur Biophys J. 2008 Sep;37(7):1207-17
18351330
J Cell Biochem. 1984;24(3):243-59
6376526
Science. 2001 Jun 29;292(5526):2492-5
11431568
Biochemistry. 1997 Dec 9;36(49):15269-76
9398255
Biochim Biophys Acta. 2007 Jan;1767(1):56-65
17123461
Photosynth Res. 2009 Feb;99(2):115-25
19199074
Biochim Biophys Acta. 2009 Dec;1787(12):1444-50
19555655
Biochim Biophys Acta. 2009 Dec;1787(12):1468-78
19576167
Biochim Biophys Acta. 1998 Oct 5;1367(1-3):88-106
9784616
Photosynth Res. 2001;68(2):141-52
16228337
Photosynth Res. 1996 Feb;47(2):175-85
24301825
Biochim Biophys Acta. 1999 Jun 30;1412(2):149-72
10393258
Biochim Biophys Acta. 1967 Jul 5;143(1):108-28
6048849
Biochemistry. 1999 Dec 21;38(51):16866-75
10606520
Biochim Biophys Acta. 2007 Jan;1767(1):106-13
17189610
Appl Environ Microbiol. 1998 Jan;64(1):337-41
16349490
Eur Biophys J. 2008 Sep;37(7):1175-84
18351331