title: Nucleosome rotational setting is associated with transcriptional regulation in promoters of tissue-specific human genes.
abstract: BACKGROUND
  The position of a nucleosome, both translational along the DNA molecule and rotational between the histone core and the DNA, is controlled by many factors, including the regular occurrence of specific dinucleotides with a period of approximately 10 bp, important for the rotational setting of the DNA around the histone octamer.
  
  
  RESULTS
  We show that such a 10 bp periodic signal of purine-purine dinucleotides occurs in phase with the transcription start site (TSS) of human genes and is centered on the position of the first (+1) nucleosome downstream of the TSS. These data support a direct link between transcription and the rotational setting of the nucleosome. The periodic signal is most prevalent in genes that contain CpG islands that are expressed at low levels in a tissue-specific manner and are involved in the control of transcription.
  
  
  CONCLUSIONS
  These results, together with several lines of evidence from the recent literature, support a new model whereby the +1 nucleosome could be more efficiently disassembled from gene promoters by H3K56 acetylation marks if the periodic signal specifies an optimal rotational setting.
authors.affiliation: Dyogen Group, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), 46 rue d'Ulm, CNRS UMR8197, INSERM U1024, 75005 Paris Cedex 05, France. charles.hebert@gmail.com
authors.forename: Charles
authors.initials: C
authors.lastname: Hebert
authors.forename: Hugues
authors.initials: H
authors.lastname: Roest Crollius
mesh_terms: D000107:Acetylation; D020029:Base Pairing / Q000235:genetics; D017124:Conserved Sequence; D018899:CpG Islands / Q000235:genetics; D004247:DNA / Q000235:genetics; D050881:E1A-Associated p300 Protein / Q000378:metabolism; D056890:Eukaryota / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016678:Genome / Q000235:genetics; D006657:Histones / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000378:metabolism; D008957:Models, Genetic; D009707:Nucleosomes / Q000235:genetics*; D009711:Nucleotides / Q000235:genetics; D009928:Organ Specificity / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012319:RNA Polymerase II / Q000378:metabolism; D012399:Rotation; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D006657:Histones; D009707:Nucleosomes; D009711:Nucleotides; D004247:DNA; D050881:E1A-Associated p300 Protein; C494360:EP300 protein, human; D012319:RNA Polymerase II; D008239:Lysine
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