MicroRNA expression in the embryonic mouse inner ear.
Although microRNA (miRNA) is expressed extensively in the postnatal mouse inner ear, its expression in the sensory epithelium during embryogenesis has not been well characterized. We investigated miRNA expression at E13.5 and E16.5 by microarray analysis, quantitative real-time-PCR, and in-situ hybridization. MiRNA-182, miRNA-140, miRNA-200c, and others showed distinct temporal and spatial expression patterns. MiRNA-194, whose expression in zebrafish seems to play an important role in the differentiation of the intestinal epithelium, was also expressed in the spiral ganglia of the mouse inner ear, where it may play a similar role in neuronal differentiation. Our results indicate that miRNAs are widely expressed in the developing inner ear, with more species recruited as hair cells differentiate, suggesting an important developmental role.
Wang
Xian-Ren
XR
Department of Otorhinolaryngology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, PR China.
Zhang
Xue-Mei
XM
Zhen
Jing
J
Zhang
Pen-Xing
PX
Xu
Geng
G
Jiang
Hongyan
H
D000465:Algorithms; D000818:Animals; D007758:Ear, Inner / Q000196:embryology* / Q000378:metabolism*; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000378:metabolism*; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012984:Software; D013136:Spiral Ganglion / Q000196:embryology / Q000378:metabolism
C547828:MIRN140 microRNA, mouse; D035683:MicroRNAs; C540524:Mirn182 microRNA, mouse