title: Detection of EBV, HBV, HCV, HIV-1, HTLV-I and -II, and SMRV in human and other primate cell lines.
abstract: The high prevalence of contaminated cell cultures suggests that viral contaminations might be distributed among cultures. We investigated more than 460 primate cell lines for Epstein-Barr (EBV), hepatitis B (HBV), hepatitis C (HCV), human immunodeficiency virus type 1 (HIV-1), human T-cell leukemia/lymphoma virus I and II (HTLV-I/-II), and squirrel monkey retrovirus (SMRV) infections for risk assessment. None of the cell lines were infected with HCV, HIV-1, or HTLV-I/-II. However, one cell line displayed reverse transcriptase activity. Thirty-nine cell lines harbored EBV DNA sequences. Studies on the lytic phase of EBV revealed that five cell lines produce EBV particles and six further cell lines produced EBV upon stimulation. One cell line contained an integrated HBV genome fragment but showed no virus production. Six cell lines were SMRV-infected. Newly established cell lines should be tested for EBV infections to detect B-lymphoblastoid cell lines (B-LCL). B-LCLs established with EBV from cell line B95-8 should be tested for SMRV infections.
authors.affiliation: Department of Human and Animal Cell Lines, German Collection of Microorganisms and Cell Cultures DSMZ, Inhoffenstrasse 7b, 38124 Braunschweig, Germany. cup@dsmz.de
authors.forename: Cord C
authors.initials: CC
authors.lastname: Uphoff
authors.forename: Sabine A
authors.initials: SA
authors.lastname: Denkmann
authors.forename: Klaus G
authors.initials: KG
authors.lastname: Steube
authors.forename: Hans G
authors.initials: HG
authors.lastname: Drexler
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D004270:DNA, Circular / Q000032:analysis; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000302:isolation & purification; D015367:Human T-lymphotropic virus 2 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction; D011323:Primates / Q000821:virology*; D015301:Retroviruses, Simian / Q000235:genetics / Q000302:isolation & purification; D012453:Saimiri / Q000821:virology; D014764:Viral Proteins / Q000032:analysis; D014780:Viruses / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D004270:DNA, Circular; D014764:Viral Proteins
references.citation: J Hepatol. 1998 Mar;28(3):396-403
references.pmid: 9551676
references.citation: Leuk Res. 2002 Apr;26(4):329-33
references.pmid: 11839374
references.citation: Virology. 1980 Mar;101(2):553-7
references.pmid: 6244705
references.citation: Proc Natl Acad Sci U S A. 1983 Apr;80(7):1987-91
references.pmid: 6300885
references.citation: J Virol. 2002 Jun;76(11):5612-26
references.pmid: 11991990
references.citation: Leukemia. 1998 Nov;12(11):1806-9
references.pmid: 9823957
references.citation: Int J Cancer. 1992 Sep 30;52(3):451-4
references.pmid: 1339415
references.citation: Int J Cancer. 1997 Nov 14;73(4):562-9
references.pmid: 9389573
references.citation: Leukemia. 1991 May;5(5):399-407
references.pmid: 1851909
references.citation: Eur J Biochem. 1987 Jun 1;165(2):393-401
references.pmid: 2439332
references.citation: DNA Cell Biol. 1998 May;17(5):415-25
references.pmid: 9628585
references.citation: Clin Exp Immunol. 1988 Jul;73(1):23-8
references.pmid: 3262465
references.citation: J Virol Methods. 2006 Sep;136(1-2):193-9
references.pmid: 16806502
references.citation: Leuk Res. 1990;14(4):381-7
references.pmid: 2159091
references.citation: Proc Natl Acad Sci U S A. 1987 Feb;84(4):1005-9
references.pmid: 3029758
references.citation: Biochem Biophys Res Commun. 1999 Oct 14;264(1):33-6
references.pmid: 10527836
references.citation: Gene. 1984 Mar;27(3):279-88
references.pmid: 6329907
references.citation: Adv Cancer Res. 2007;97:81-109
references.pmid: 17419942
references.citation: Eur J Haematol. 1999 Jan;62(1):1-18
references.pmid: 9918306
references.citation: J Virol Methods. 1992 Dec 1;40(3):347-56
references.pmid: 1282132
references.citation: Leuk Lymphoma. 2004 Mar;45(3):567-74
references.pmid: 15160920
references.citation: J Virol. 1990 Mar;64(3):1143-55
references.pmid: 2154599
references.citation: Neoplasma. 1974;21(6):619-27
references.pmid: 4141066
references.citation: J Hepatol. 1999 Nov;31(5):967-8
references.pmid: 10580600
references.citation: Virology. 1988 Dec;167(2):468-76
references.pmid: 3201749
references.citation: J Virol Methods. 1992 Sep;39(3):259-68
references.pmid: 1430070
references.citation: Int J Cancer. 1987 Jan 15;39(1):89-93
references.pmid: 3098690
references.citation: Int J Cancer. 1982 Jul 15;30(1):93-9
references.pmid: 6288581
references.citation: Int J Cancer. 1974 Mar 15;13(3):363-76
references.pmid: 4362611
references.citation: Beitr Pathol. 1975 May;155(1):72-8
references.pmid: 168861
references.citation: Proc Natl Acad Sci U S A. 1987 Mar;84(5):1332-6
references.pmid: 3029778
references.citation: Nature. 1973 Mar 2;242(5392):44-7
references.pmid: 4348458
references.citation: Cell Mol Biol. 1986;32(3):343-50
references.pmid: 3017561
references.citation: Leukemia. 1994 Apr;8(4):652-8
references.pmid: 8152260
references.citation: Br J Haematol. 1998 Sep;102(4):1081-9
references.pmid: 9734661
references.citation: J Med Virol. 1990 Apr;30(4):266-71
references.pmid: 2164560
references.citation: Braz J Med Biol Res. 2004 Nov;37(11):1707-12
references.pmid: 15517087
references.citation: ALTEX. 2005;22(2):103-9
references.pmid: 15953965
references.citation: Nature. 1981 Dec 24;294(5843):770-1
references.pmid: 6275274
references.citation: Eur J Cancer. 1999 Dec;35(14):1878-85
references.pmid: 10711230
references.citation: Genes Chromosomes Cancer. 1992 Apr;4(3):205-10
references.pmid: 1382560
references.citation: Leukemia. 1994 Sep;8(9):1510-26
references.pmid: 8090031
references.citation: Gastroenterology. 1993 Nov;105(5):1529-33
references.pmid: 8224658
references.citation: J Virol. 1984 Apr;50(1):248-54
references.pmid: 6321792
references.citation: Leukemia. 1991 Mar;5(3):221-4
references.pmid: 1849602
references.citation: J Infect Dis. 1988 Dec;158(6):1193-7
references.pmid: 3198935
references.citation: J Exp Med. 1989 Jun 1;169(6):2191-8
references.pmid: 2543732
references.citation: Science. 1977 Jan 21;195(4275):289-92
references.pmid: 63993
references.citation: Leukemia. 1994 Aug;8(8):1385-91
references.pmid: 8057678
references.citation: Haemophilia. 2002 May;8(3):236-43
references.pmid: 12010417
references.citation: In Vitro Cell Dev Biol Anim. 2002 Feb;38(2):79-85
references.pmid: 11928999
references.citation: Blood. 1987 Nov;70(5):1665-72
references.pmid: 3478106