Proteomic analysis of human ovarian cancer paclitaxel-resistant cell lines.
OBJECTIVE
To study the proteins related to paclitaxel-resistant of ovarian cancer cell line.


METHODS
The total proteins of paclitaxel-resistant and paclitaxel-sensitive human ovarian cancer cell lines were separated by 2-dimensional gel electrophoresis (2-DE). The differentially expressed proteins were analyzed using image analysis software. The differential proteins were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Western blot was used to determine the differential expression levels of the 2 proteins.


RESULTS
Forty differentially expressed proteins were found by image analysis software, and 24 differential proteins were identified by mass spectrometry. These proteins included proliferation cell nuclear antigen (PCNA), nm23, prohibitin (PHB), annexin, alpha-enolase, heat shock protein (HSP), and so on.


CONCLUSIONS
Twenty-four proteins in human ovarian cancer cell lines of paclitaxel-resistant and paclitaxel-sensitive are found by proteomic techniques, which may be involved in the paclitaxel-resistance of human ovarian cancer cells.
Cao
Lanqin
L
Department of Gynecology and Obstetrics, Xiangya Hospital, Central South University, Changsha 410008, China. caolanqin@163.com
Li
Xin
X
Zhang
Yi
Y
Peng
Fang
F
Yi
Hong
H
Xu
Yan
Y
Li
Xinguo
X
Wang
Qian
Q
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D054778:NM23 Nucleoside Diphosphate Kinases / Q000032:analysis; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017239:Paclitaxel / Q000494:pharmacology*; D000091402:Prohibitins; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012097:Repressor Proteins / Q000032:analysis
D000972:Antineoplastic Agents, Phytogenic; D054778:NM23 Nucleoside Diphosphate Kinases; C000717328:PHB protein, human; D000091402:Prohibitins; D018809:Proliferating Cell Nuclear Antigen; D020543:Proteome; D012097:Repressor Proteins; C517938:NME1 protein, human; D017239:Paclitaxel