title: Validation of novel promoter sequences derived from two endogenous ubiquitin genes in transgenic Aedes aegypti.
abstract: To date, only a limited number of promoter sequences have been described to drive transgene expression in the disease vector Aedes aegypti. We sought to increase this repertoire by characterizing the ability of upstream sequences derived from the Ae. aegypti Ub(L40) and polyubiquitin genes to drive the expression of marker proteins. Both genomic fragments were able to drive robust expression of luciferase in cultured mosquito cells. Following Mos1-transformation, the Ub(L40) promoter drove strong expression of a fluorescent marker in early larvae and in ovaries, while the polyubiquitin promoter drove robust EGFP expression in all stages of development, including constitutive expression throughout the midgut. These promoter fragments provide two new expression profiles for future Ae. aegypti genetic experiments.
authors.affiliation: Fralin Life Science Institute and Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
authors.forename: M A E
authors.initials: MA
authors.lastname: Anderson
authors.forename: T L
authors.initials: TL
authors.lastname: Gross
authors.forename: K M
authors.initials: KM
authors.lastname: Myles
authors.forename: Z N
authors.initials: ZN
authors.lastname: Adelman
mesh_terms: D000330:Aedes / Q000166:cytology / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D005786:Gene Expression Regulation; D017344:Genes, Insect / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D025821:Polyubiquitin / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015203:Reproducibility of Results; D014170:Transformation, Genetic; D025801:Ubiquitin / Q000235:genetics*
chemical_list: D025801:Ubiquitin; C485184:enhanced green fluorescent protein; D025821:Polyubiquitin; D049452:Green Fluorescent Proteins
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