title: HOXA9 regulates miR-155 in hematopoietic cells.
abstract: HOXA9-mediated up-regulation of miR-155 was noted during an array-based analysis of microRNA expression in Hoxa9(-/-)bone marrow (BM) cells. HOXA9 induction of miR-155 was confirmed in these samples, as well as in wild-type versus Hoxa9-deficient marrow, using northern analysis and qRT-PCR. Infection of wild-type BM with HOXA9 expressing or GFP(+) control virus further confirmed HOXA9-mediated regulation of miR-155. miR-155 expression paralleled Hoxa9 mRNA expression in fractionated BM progenitors, being highest in the stem cell enriched pools. HOXA9 capacity to induce myeloid colony formation was blunted in miR-155-deficient BM cells, indicating that miR-155 is a downstream mediator of HOXA9 function in blood cells. Pu.1, an important regulator of myelopoiesis, was identified as a putative down stream target for miR-155. Although miR-155 was shown to down-regulate the Pu.1 protein, HOXA9 did not appear to modulate Pu.1 expression in murine BM cells.
authors.affiliation: Department of Medicine, Department of Veterans Affairs Medical Center and University of California, San Francisco, CA 94143, USA.
authors.forename: Yu-Long
authors.initials: YL
authors.lastname: Hu
authors.forename: Stephen
authors.initials: S
authors.lastname: Fong
authors.forename: Corey
authors.initials: C
authors.lastname: Largman
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Shen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D015854:Up-Regulation; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D018398:Homeodomain Proteins; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C532623:Mirn155 microRNA, mouse; C097899:homeobox protein HOXA9
references.citation: Mol Cancer Res. 2008 Dec;6(12):1830-40
references.pmid: 19074828
references.citation: Mol Cell Biol. 1997 Nov;17(11):6448-58
references.pmid: 9343407
references.citation: Curr Opin Genet Dev. 1998 Aug;8(4):423-9
references.pmid: 9729718
references.citation: Mol Cell Biol. 2000 May;20(9):3274-85
references.pmid: 10757811
references.citation: J Cell Biochem. 2008 Jun 1;104(3):805-17
references.pmid: 18189265
references.citation: Mol Cell Biol. 2008 Jul;28(14):4609-19
references.pmid: 18474618
references.citation: EMBO J. 2001 Feb 1;20(3):350-61
references.pmid: 11157742
references.citation: Ann Rheum Dis. 2008 Dec;67 Suppl 3:iii50-5
references.pmid: 19022814
references.citation: Cell. 2003 Dec 26;115(7):787-98
references.pmid: 14697198
references.citation: Mol Cell Biol. 1999 Nov;19(11):7577-88
references.pmid: 10523646
references.citation: Genome Res. 2008 Nov;18(11):1787-97
references.pmid: 18849523
references.citation: Blood. 1997 Mar 15;89(6):1922-30
references.pmid: 9058712
references.citation: Immunity. 2007 Dec;27(6):847-59
references.pmid: 18055230
references.citation: J Biol Chem. 1997 Mar 28;272(13):8198-206
references.pmid: 9079637
references.citation: Biochem Soc Trans. 2008 Jun;36(Pt 3):531-3
references.pmid: 18481999
references.citation: Blood. 2009 Aug 13;114(7):1374-82
references.pmid: 19520806
references.citation: Semin Oncol. 2006 Apr;33(2):167-73
references.pmid: 16616063
references.citation: Leukemia. 2002 Feb;16(2):186-95
references.pmid: 11840284
references.citation: Proc Natl Acad Sci U S A. 2005 Mar 8;102(10):3627-32
references.pmid: 15738415
references.citation: Blood. 1998 Jul 15;92(2):383-93
references.pmid: 9657735
references.citation: Blood. 2007 Jun 1;109(11):4732-8
references.pmid: 17327400
references.citation: EMBO J. 1998 Jul 1;17(13):3714-25
references.pmid: 9649441
references.citation: Blood. 2004 Mar 1;103(5):1676-84
references.pmid: 14604967
references.citation: Circ Res. 2004 Apr 2;94(6):743-51
references.pmid: 14764452
references.citation: Leuk Lymphoma. 2009 Feb;50(2):160-70
references.pmid: 19160125
references.citation: Science. 2007 Apr 27;316(5824):604-8
references.pmid: 17463289
references.citation: Leukemia. 1999 Dec;13(12):1993-9
references.pmid: 10602420
references.citation: Genes Dev. 1995 Mar 15;9(6):663-74
references.pmid: 7729685
references.citation: J Biol Chem. 2005 Apr 1;280(13):12359-70
references.pmid: 15681849
references.citation: EMBO J. 1998 Mar 2;17(5):1434-45
references.pmid: 9482740
references.citation: Dev Biol. 1997 Jan 15;181(2):186-96
references.pmid: 9013929
references.citation: Mol Cell Biol. 1999 Apr;19(4):3051-61
references.pmid: 10082572
references.citation: Mol Cell Biol. 2009 Sep;29(18):5181-92
references.pmid: 19620287
references.citation: Development. 1996 Feb;122(2):461-72
references.pmid: 8625797
references.citation: Blood. 2005 Feb 15;105(4):1456-66
references.pmid: 15522959
references.citation: Blood. 2005 Dec 1;106(12):3988-94
references.pmid: 16091451
references.citation: Leukemia. 2009 Jul;23(7):1257-63
references.pmid: 19148134
references.citation: Int J Biochem Cell Biol. 2008;40(1):22-7
references.pmid: 17374502
references.citation: Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):541-6
references.pmid: 9892669
references.citation: Nature. 2003 Oct 30;425(6961):926-33
references.pmid: 14586461
references.citation: Nat Med. 2005 May;11(5):531-7
references.pmid: 15821746
references.citation: Science. 2004 Jan 2;303(5654):83-6
references.pmid: 14657504
references.citation: Eur J Haematol. 2008 Oct;81(4):304-10
references.pmid: 18573170
references.citation: Cell. 2006 Jul 28;126(2):415-26
references.pmid: 16873070
references.citation: Science. 1999 Oct 15;286(5439):531-7
references.pmid: 10521349
references.citation: Genome Biol. 2005;6(8):R71
references.pmid: 16086853
references.citation: Exp Hematol. 2007 Apr;35(4):551-64
references.pmid: 17379065
references.citation: Blood. 1996 Aug 15;88(4):1234-47
references.pmid: 8695841
references.citation: Leukemia. 1999 May;13(5):687-98
references.pmid: 10374871
references.citation: Curr Opin Hematol. 2008 Jul;15(4):352-8
references.pmid: 18536574
references.citation: J Exp Med. 2008 Mar 17;205(3):585-94
references.pmid: 18299402