title: Two-hybrid analysis of Ty3 capsid subdomain interactions.
abstract: BACKGROUND
  The yeast retrotransposon Ty3 forms stable virus-like particles. Gag3, the major structural protein, is composed of capsid, spacer and nucleocapsid domains. The capsid domain of Gag3 was previously modeled as a structure similar to retrovirus capsid.
  
  
  RESULTS
  Two-hybrid analysis was used to understand the interactions that contribute to particle assembly. Gag3 interacted with itself as predicted based on its role as the major structural protein. The N-terminal subdomain (NTD) of the capsid was able to interact with itself and with the C-terminal subdomain (CTD) of the capsid, but interacted less well with intact Gag3. Mutations previously shown to block particle assembly disrupted Gag3 interactions more than subdomain interactions.
  
  
  CONCLUSIONS
  The findings that the NTD interacts with itself and with the CTD are consistent with previous modeling and a role similar to that of the capsid in retrovirus particle structure. These results are consistent with a model in which the Gag3-Gag3 interactions that initiate assembly differ from the subdomain interactions that potentially underlie particle stability.
authors.affiliation: Department of Biological Chemistry, University of California, Irvine, CA, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Biological Chemistry, University of California, Irvine, CA, USA.
authors.forename: Liza Sz
authors.initials: LS
authors.lastname: Larsen
authors.affiliation: Department of Biological Chemistry, University of California, Irvine, CA, USA.
authors.forename: Becky
authors.initials: B
authors.lastname: Irwin
authors.affiliation: Department of Biological Chemistry, University of California, Irvine, CA, USA.
authors.forename: Virginia
authors.initials: V
authors.lastname: Bilanchone
authors.affiliation: Department of Biological Chemistry, University of California, Irvine, CA, USA.|Department of Microbiology and Molecular Genetics, University of California, Irvine, CA, USA.|Institute for Genomics and Bioinformatics, University of California, Irvine, CA, USA.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Sandmeyer
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