title: Screening and sequence analysis of the hemolysin gene of Fusobacterium necrophorum.
abstract: Fusobacterium necrophorum is the main pathogen that causes numerous necrobacilloses. Hemolysin is one of the major virulence factors involved in fusobacterial infections. In order to investigate the genetic basis of hemolytic activity and the regulation mechanism of the hemolysin expression, a genomic library was constructed from F. necrophorum DNA by ligating DNA fragments generated by partial HindIII digestion with pUC18 vector. The screening of the genomic library with polymerase chain reaction, DNA sequencing and sequence assembly led to a 7.45 kb sequence which includes the putative hly gene and upstream sequence. Clustered putative genes encoding short chain acyl-CoA dehydrogenase (Scad) and electron transfer flavoprotein (Etf) alpha and beta subunits locate upstream of hly. A 535 bp non-coding sequence, possibly with some cis-regulatory elements involved in the regulation of the hemolysin expression in F. necrophorum, locates between etf-beta and hly. The nucleotide sequence of the hly gene indicates it encodes hemolysin. It is the first characterized hemolysin coding gene in F. necrophorum.
authors.affiliation: Institute of Special Wild Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 15 Luming Street, Jilin, China. Mliguang66@sohu.com
authors.forename: Liguang
authors.initials: L
authors.lastname: Miao
authors.forename: Yanhuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Qingchao
authors.initials: Q
authors.lastname: Li
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.forename: Guanglei
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D005675:Fusobacterium necrophorum / Q000235:genetics*; D015723:Gene Library; D023061:Gene Order; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D006460:Hemolysin Proteins