title: The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow.
abstract: Through the preparation of a novel controlled pore glass-poly(pyrrole) material we have developed a conducting support that is not only suitable for the co-immobilisation of enzymes and co-factors, but also enables the facile electrochemical regeneration of the co-factor during a reaction. Employing the selective reduction of (rac)-2-phenylpropionaldehyde to (S)-phenyl-1-propanol as a model, we have demonstrated the successful co-immobilisation of the HLADH enzyme and co-factor NAD(H); with incorporation of the material into a continuous flow reactor facilitating the in situ electrochemical regeneration of NAD(H) for in excess of 100 h. Using this approach we have developed a reagent-less, atom efficient system applicable to the cost-effective, continuous biosynthesis of chiral compounds.
authors.affiliation: Department of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
authors.forename: B
authors.initials: B
authors.lastname: Ngamsom
authors.forename: A M
authors.initials: AM
authors.lastname: Hickey
authors.forename: G M
authors.initials: GM
authors.lastname: Greenway
authors.forename: J A
authors.initials: JA
authors.lastname: Littlechild
authors.forename: T
authors.initials: T
authors.lastname: McCreedy
authors.forename: P
authors.initials: P
authors.lastname: Watts
authors.forename: C
authors.initials: C
authors.lastname: Wiles
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D055162:Biocatalysis; D004553:Electric Conductivity; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005898:Glass / Q000737:chemistry; D007700:Kinetics; D009243:NAD / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D011758:Pyrroles / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D011758:Pyrroles; D009243:NAD; C067635:polypyrrole; D000426:Alcohol Dehydrogenase