title: An efficient method for the expression and reconstitution of thermostable Mn/Fe superoxide dismutase from Aeropyrum pernix K1.
abstract: The gene APE0743 encoding the superoxide dismutase (ApSOD) of a hyperthermophilic archaeon Aeropyrum pernix K1 was cloned and over-expressed as a GST fusion protein at a high level in Escherichia coli. The expressed protein was simply purified by the process of glutathione affinity chromatography and thrombin treatment. The ApSOD was a homodimer of 25 kDa subunits and a cambialistic SOD which was active with either Fe(II) or Mn(II) as a cofactor. The ApSOD was highly stable against high temperature. This thermostable ApSOD is expected to be applicable as a useful biocatalyst for medicine and bio-industrial processes.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Chung-Ang University, Seoul, Korea.
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Hye-Won
authors.initials: HW
authors.lastname: Kwon
authors.forename: Jong-Uk
authors.initials: JU
authors.lastname: Koh
authors.forename: Dong-Kuk
authors.initials: DK
authors.lastname: Lee
authors.forename: Ja-Young
authors.initials: JY
authors.lastname: Moon
authors.forename: Kwang-Hoon
authors.initials: KH
authors.lastname: Kong
mesh_terms: D041522:Aeropyrum / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007218:Industrial Microbiology / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D016415:Sequence Alignment; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D019641:DNA, Archaeal; D011994:Recombinant Proteins; D013482:Superoxide Dismutase