Association of TGF-beta1 gene polymorphisms in exon1 and blood levels with essential hypertension.
OBJECTIVE
Based on a case-control study, we investigated the relationship between +869T/C and +915G/C gene polymorphisms in transforming growth factor-beta1 (TGF-beta1), protein levels and essential hypertension (EH) in the Kazakh and Han Chinese populations selected from the Boertonggu countryside of Shawan region in the Xinjiang Uygur Autonomous Region of China (n=1600). The polymorphisms of TGF-beta1 and the blood levels were detected using polymerase chain reaction-restriction fragment length polymorphism assays and sandwich ELISA, respectively.


RESULTS
An association was found between +869C-allele with higher risk of EH in these two populations. We also found that the CG haplotype of the two polymorphisms was associated with EH in the Kazakh EH patients. The levels of TGF-beta(1) in the blood were positively correlated with diastolic blood pressure both in the Kazakh and Han EH patients. Levels of the TGF-beta1 protein in the Kazakh EH patients were significantly higher than those in the Han EH patients.


CONCLUSIONS
These results suggest that the TGF-beta1 +869 C allele is potentially a genetic factor of EH in these two ethnicities, the CG haplotype can be a genetic marker of EH in the Kazakh Chinese and the high concentration of TGF-beta1 is possibly associated with EH, especially in the Kazakh population.
He
Fang
F
Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology/Key Laboratory of Respiratory Diseases, Health Ministry of China, Wuhan, China.
Zhao
Dan
D
Deng
Fengmei
F
Zhong
Hua
H
Shi
Xiaopeng
X
Yang
Jianfeng
J
Guo
Shuxia
S
Cheng
Jiang
J
Huang
Gang
G
Tang
Bin
B
Wang
Zhenhuan
Z
Chen
Xiongying
X
Wang
Gang
G
Zhang
Wanjiang
W
Zhang
Chunjun
C
Wang
Xuewen
X
Hu
Qinghua
Q
D000293:Adolescent; D000328:Adult; D000368:Aged; D001794:Blood Pressure / Q000235:genetics; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005091:Exons; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000453:epidemiology / Q000235:genetics*; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D016212:Transforming Growth Factor beta / Q000097:blood* / Q000235:genetics*; D055815:Young Adult
D016212:Transforming Growth Factor beta