Immunohistochemical localization and mRNA expression of aquaporins in the macula utriculi of patients with Meniere's disease and acoustic neuroma.
Meniere's disease is nearly invariably associated with endolymphatic hydrops (the net accumulation of water in the inner ear endolymphatic space). Vestibular maculae utriculi were acquired from patients undergoing surgery for Meniere's disease and acoustic neuroma and from autopsy (subjects with normal hearing and balance). Quantitative immunostaining was conducted with antibodies against aquaporins (AQPs) 1, 4, and 6, Na(+)K(+)ATPase, Na(+)K(+)2Cl co-transporter (NKCC1), and alpha-syntrophin. mRNA was extracted from the surgically acquired utricles from subjects with Meniere's disease and acoustic neuroma to conduct quantitative real-time reverse transcription with polymerase chain reaction for AQP1, AQP4, and AQP6. AQP1 immunoreactivity (-IR) was located in blood vessels and fibrocytes in the underlying stroma, without any apparent alteration in Meniere's specimens when compared with acoustic neuroma and autopsy specimens. AQP4-IR localized to the epithelial basolateral supporting cells in Meniere's disease, acoustic neuroma, and autopsy. In specimens from subjects with Meniere's disease, AQP4-IR was significantly decreased compared with autopsy and acoustic neuroma specimens. AQP6-IR occurred in the sub-apical vestibular supporting cells in acoustic neuroma and autopsy samples. However, in Meniere's disease specimens, AQP6-IR was significantly increased and diffusely redistributed throughout the supporting cell cytoplasm. Na(+)K(+)ATPase, NKCC1, and alpha-syntrophin were expressed within sensory epithelia and were unaltered in Meniere's disease specimens. Expression of AQP1, AQP4, or AQP6 mRNA did not differ in vestibular endorgans from patients with Meniere's disease. Changes in AQP4 (decreased) and AQP6 (increased) expression in Meniere's disease specimens suggest that the supporting cell might be a cellular target.
Ishiyama
Gail
G
Neurology Department, Division of Head and Neck, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA. ishiyama@ucla.edu
Lopez
Ivan A
IA
Beltran-Parrazal
Luis
L
Ishiyama
Akira
A
D008267:Acoustic Maculae / Q000378:metabolism* / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D020346:Aquaporins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006706:Homeostasis; D006801:Humans; D007150:Immunohistochemistry; D007477:Ions; D008297:Male; D008575:Meniere Disease / Q000150:complications* / Q000235:genetics*; D008875:Middle Aged; D009464:Neuroma, Acoustic / Q000150:complications* / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020346:Aquaporins; D007477:Ions; D020033:Protein Isoforms; D012333:RNA, Messenger
Neuron. 2000 May;26(2):417-30
10839360
Ann Otol Rhinol Laryngol. 1989 Nov;98(11):873-83
2817679
Brain Res. 2009 Feb 9;1253:27-34
19070604
Curr Opin Otolaryngol Head Neck Surg. 2006 Oct;14(5):332-6
16974147
J Biol Chem. 2001 Aug 17;276(33):31233-7
11406631
Hum Mol Genet. 1999 Aug;8(8):1579-84
10401008
J Biol Chem. 2002 Oct 18;277(42):39873-9
12177001
Otol Neurotol. 2005 Jan;26(1):74-81
15699723
J Assoc Res Otolaryngol. 2003 Jun;4(2):264-75
12943377
Curr Opin Otolaryngol Head Neck Surg. 2004 Oct;12(5):408-12
15377953
Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):14108-13
11717465
Biochem Biophys Res Commun. 2002 Oct 4;297(4):987-96
12359252
Physiology (Bethesda). 2006 Oct;21:336-45
16990454
Cell Tissue Res. 2007 Jun;328(3):453-60
17318586
Tohoku J Exp Med. 2002 Apr;196(4):247-57
12086153
Otol Neurotol. 2008 Jan;29(1):33-8
18199956
Eur J Neurosci. 1998 Dec;10(12):3584-95
9875338
Nature. 1999 Nov 11;402(6758):184-7
10647010
Am J Physiol. 1995 Dec;269(6 Pt 1):C1450-6
8572173
Neuroscience. 2004;129(4):999-1010
15561415
Arch Histol Cytol. 2001 Aug;64(3):329-38
11575429
Am J Physiol Cell Physiol. 2007 Oct;293(4):C1187-208
17670895
PLoS One. 2009;4(2):e4471
19214237
Hear Res. 1997 Dec;114(1-2):21-34
9447915
Curr Opin Neurol. 2004 Feb;17(1):9-16
15090872
Nat Genet. 1999 Jun;22(2):192-5
10369265
Nat Rev Neurosci. 2003 Dec;4(12):991-1001
14682361
Hear Res. 2002 Mar;165(1-2):85-95
12031518
Cell Mol Neurobiol. 2003 Jun;23(3):315-29
12825830
Neurochem Int. 2004 Dec;45(7):963-70
15337294
Hear Res. 2002 Sep;171(1-2):51-65
12204349
Ann N Y Acad Sci. 2002 May;962:260-74
12076980
Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):2106-11
12578959
J Histochem Cytochem. 1997 Jun;45(6):773-8
9199662
J Physiol. 2002 Jul 1;542(Pt 1):3-16
12096044
Pediatr Res. 2005 May;57(5 Pt 2):47R-53R
15817503
Acta Otolaryngol. 2008 Aug;128(8):832-40
18607959
Hear Res. 1999 Jun;132(1-2):76-84
10392550
Proteomics. 2006 Oct;6(20):5637-49
17044001
Curr Opin Otolaryngol Head Neck Surg. 2005 Oct;13(5):301-7
16160525
Laryngoscope. 1987 Aug;97(8 Pt 1):984-91
3613802
BMC Ear Nose Throat Disord. 2009 Jun 03;9:4
19493357
J Neurosurg. 2010 May;112(5):1095-104
19731985
Proc R Soc Med. 1938 Sep;31(11):1317-36
19991672
Hear Res. 2009 Aug;254(1-2):1-14
19348877
BMC Neurosci. 2009 May 27;10:53
19580685
Neuroreport. 2002 Jul 2;13(9):1127-9
12151754
Physiol Rev. 2000 Jan;80(1):211-76
10617769
Am J Otolaryngol. 1982 Sep-Oct;3(5):353-60
6293330
Methods. 2001 Dec;25(4):402-8
11846609
J Biomed Biotechnol. 2010;2010:264704
20111742
Am J Physiol Renal Physiol. 2000 Dec;279(6):F1014-26
11097619
J Neurosci. 2006 Jul 26;26(30):7811-9
16870726
J Biol Chem. 1999 Sep 17;274(38):26946-55
10480906
Acta Otolaryngol Suppl. 1984;406:10-25
6591683
Am J Physiol Renal Physiol. 2000 Jan;278(1):F13-28
10644652
Trends Pharmacol Sci. 2002 Dec;23(12):558-62
12457773
Arch Neurol. 2001 Jul;58(7):1151-6
11448308
Hear Res. 2007 Jan;223(1-2):93-104
17158005
Nephrology (Carlton). 2007 Dec;12(6):553-8
17995580
Otolaryngol Head Neck Surg. 1995 Sep;113(3):181-5
7675476