Carbon metabolism of intracellular bacterial pathogens and possible links to virulence.
New technologies such as high-throughput methods and 13C-isotopologue-profiling analysis are beginning to provide us with insight into the in vivo metabolism of microorganisms, especially in the host cell compartments that are colonized by intracellular bacterial pathogens. In this Review, we discuss the recent progress made in determining the major carbon sources and metabolic pathways used by model intracellular bacterial pathogens that replicate either in the cytosol or in vacuoles of infected host cells. Furthermore, we highlight the possible links between intracellular carbon metabolism and the expression of virulence genes.
Eisenreich
Wolfgang
W
Lehrstuhl für Biochemie, Technische Universität München, D-85747 Garching, Germany.
Dandekar
Thomas
T
Heesemann
Jürgen
J
Goebel
Werner
W
D001419:Bacteria / Q000378:metabolism* / Q000472:pathogenicity; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D003600:Cytosol / Q000382:microbiology; D044404:Genomic Islands; D054884:Host-Pathogen Interactions; D008089:Listeria monocytogenes / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism; D012485:Salmonella typhi / Q000378:metabolism / Q000472:pathogenicity; D012763:Shigella flexneri / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D014617:Vacuoles / Q000382:microbiology; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D010454:Peptide Termination Factors; C545356:PrfA protein, Listeria monocytogenes; D037521:Virulence Factors; D002244:Carbon
Microbiology (Reading). 2007 Apr;153(Pt 4):1221-1228
17379731
Nucleic Acids Res. 2002 Oct 15;30(20):4432-41
12384590
Nat Rev Microbiol. 2005 Jan;3(1):70-80
15608701
Infect Immun. 2005 Jan;73(1):88-102
15618144
Microbiology (Reading). 2010 Apr;156(Pt 4):1069-1083
20056707
Cell Microbiol. 2006 Jan;8(1):10-22
16367862
Nature. 2001 Oct 25;413(6858):852-6
11677609
J Bacteriol. 2009 Apr;191(7):2187-96
19181817
Mol Microbiol. 2009 Apr;72(1):41-52
19210624
Infect Immun. 2003 Dec;71(12):7002-13
14638790
BMC Microbiol. 2008 Feb 27;8:38
18304349
Nat Med. 2005 Jun;11(6):638-44
15895072
Cell Microbiol. 2007 Sep;9(9):2181-91
17490410
Eur J Biochem. 2003 Mar;270(5):880-91
12603321
Nat Rev Mol Cell Biol. 2008 Jan;9(1):72-81
17987043
Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11760-5
16868085
Mol Microbiol. 1999 Apr;32(2):223-32
10231480
Nature. 2008 Oct 23;455(7216):1054-6
18948945
Nat Rev Microbiol. 2008 Jan;6(1):53-66
18026123
PLoS One. 2008 Jan 02;3(1):e1403
18167562
FEMS Microbiol Rev. 2008 May;32(3):440-60
18266740
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11470-4
1763061
Clin Microbiol Infect. 2009 Jan;15 Suppl 1:47-50
19220355
Nat Rev Microbiol. 2009 Sep;7(9):623-8
19648949
FEMS Microbiol Rev. 1996 Dec;19(2):85-116
8988566
Am J Physiol. 1992 Sep;263(3 Pt 2):R482-8
1415631
Curr Opin Microbiol. 2005 Oct;8(5):612-9
16126452
Microbiology (Reading). 2009 Jul;155(Pt 7):2245-2255
19389781
Cell Microbiol. 2000 Jun;2(3):187-93
11207575
Scand J Gastroenterol Suppl. 1997;222:3-9
9145437
J Bacteriol. 1998 Dec;180(23):6316-24
9829942
Science. 2001 Oct 26;294(5543):849-52
11679669
J Bacteriol. 2009 Apr;191(7):2218-27
19181806
EMBO J. 2000 Jul 3;19(13):3235-49
10880437
Science. 1992 Sep 11;257(5076):1496-502
1523409
J Exp Biol. 1994 Nov;196:197-212
7823022
Science. 2003 Oct 17;302(5644):462-4
14564012
Nat Rev Microbiol. 2008 Sep;6(9):657-66
18679171
Infect Immun. 2006 Feb;74(2):1323-38
16428782
Infect Immun. 2006 Feb;74(2):1130-40
16428761
Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12989-94
14569030
Nat Rev Microbiol. 2009 May;7(5):355-66
19369951
J Microbiol. 2005 Feb;43 Spec No:85-92
15765061
Mol Microbiol. 2006 Aug;61(4):940-7
16879647
Nat Rev Microbiol. 2007 Jan;5(1):39-47
17160001
Nat Rev Microbiol. 2008 Aug;6(8):613-24
18628769
Appl Environ Microbiol. 1991 Oct;57(10):3046-8
1746963
Nat Rev Microbiol. 2007 Dec;5(12):917-27
17982469
Microbiology (Reading). 2008 Mar;154(Pt 3):679-692
18310015
Nat Rev Cancer. 2002 Jan;2(1):38-47
11902584
Res Microbiol. 1998 Jan;149(1):15-25
9766205
J Bacteriol. 2008 Aug;190(15):5412-30
18502850
Infect Immun. 2002 Aug;70(8):4379-88
12117948
Mol Microbiol. 2003 Feb;47(3):825-38
12535079
Adv Appl Microbiol. 2009;67:47-64
19245936
Curr Opin Microbiol. 2009 Feb;12(1):110-6
19157960
Microbes Infect. 2001 Jun;3(7):549-59
11418329
Nat Biotechnol. 2008 Oct;26(10):1179-86
18820684
Nat Protoc. 2007;2(4):770-7
17446876
Nature. 2008 Oct 23;455(7216):1114-8
18806773
Biosci Biotechnol Biochem. 2009 Feb;73(2):245-59
19202299
Proc Natl Acad Sci U S A. 2007 Feb 6;104(6):1947-52
17264217
Int Rev Cell Mol Biol. 2008;270:1-38
19081533
J Microbiol. 2005 Feb;43 Spec No:110-7
15765064
Microbiology (Reading). 2003 Jul;149(Pt 7):1829-1835
12855734
Science. 2009 May 22;324(5930):1029-33
19460998
Mol Microbiol. 1998 Oct;30(1):175-88
9786194
Proc Natl Acad Sci U S A. 2005 Jun 7;102(23):8327-32
15928073
Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):431-6
11756655
J Infect Dis. 2004 Feb 1;189(3):393-401
14745696
Mol Microbiol. 2006 Jun;60(5):1109-22
16689789
Mol Microbiol. 1995 Nov;18(4):715-27
8817493
Nat Protoc. 2009;4(6):878-92
19478804
Cell Host Microbe. 2008 Jun 12;3(6):399-407
18541216
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5189-93
3523484
Infect Immun. 2005 Apr;73(4):2547-9
15784602
Curr Opin Microbiol. 2000 Feb;3(1):54-9
10679413
Infect Immun. 1993 Sep;61(9):3756-60
8359896
Cell. 2002 Sep 6;110(5):551-61
12230973
Biochem Soc Trans. 2007 Jun;35(Pt 3):544-9
17511648
J Bacteriol. 2007 Jan;189(2):473-90
17085572
Exp Cell Res. 2009 Jul 1;315(11):1791-7
19332053
J Bacteriol. 2009 Jan;191(2):461-76
18978044
Curr Opin Microbiol. 2006 Apr;9(2):138-42
16459132
Nature. 1998 Jun 11;393(6685):537-44
9634230
Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4602-7
15070764
Mol Microbiol. 1999 Feb;31(3):971-82
10048039
Microbes Infect. 2007 Aug;9(10):1196-207
17764998
Mol Microbiol. 1996 Aug;21(4):823-37
8878044
Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):12221-6
11572936
Microbiology (Reading). 2005 Mar;151(Pt 3):951-962
15758240
Mol Microbiol. 2007 Aug;65(3):684-99
17635188
Cell Microbiol. 2006 Feb;8(2):177-84
16441429
PLoS One. 2010 May 11;5(5):e10586
20485672
J Bacteriol. 2006 Jan;188(2):556-68
16385046
Nat Rev Microbiol. 2009 May;7(5):333-40
19369949
Mol Microbiol. 2002 Dec;46(5):1451-64
12453229
Infect Immun. 2009 Apr;77(4):1397-405
19168732
Mol Syst Biol. 2006;2:62
17102807
Nat Protoc. 2009;4(6):799-810
19444238
J Exp Med. 2003 Sep 1;198(5):693-704
12953091
Eur J Biochem. 1995 Sep 1;232(2):433-48
7556192
Nat Rev Microbiol. 2008 Dec;6(12):883-92
18955984
Infect Immun. 2004 Oct;72(10):5622-9
15385459
Infect Immun. 2009 Jul;77(7):3117-26
19380470
Curr Opin Microbiol. 2009 Feb;12(1):31-6
19135408
Nature. 2000 Aug 17;406(6797):735-8
10963599
J Bacteriol. 2007 Jun;189(11):4265-74
17384189
Br J Nutr. 2003 Jan;89(1):3-9
12568659
Curr Opin Gastroenterol. 2008 Jan;24(1):22-6
18043228
Mol Microbiol. 2000 May;36(3):749-61
10844662
Curr Genomics. 2007 Apr;8(2):113-28
18660845
Curr Opin Microbiol. 2007 Feb;10(1):24-9
17208038
Microbiology (Reading). 2002 Sep;148(Pt 9):2705-2715
12213917
Infect Immun. 1996 Aug;64(8):3055-61
8757833
Curr Opin Microbiol. 2008 Feb;11(1):46-52
18282735
Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2040-5
16461909
Nat Cell Biol. 2009 Oct;11(10):1212-8
19767742
Int J Med Microbiol. 2001 May;291(2):171-82
11437339
Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4376-80
18334639
Trends Biotechnol. 2005 Nov;23(11):544-6
16154652
Cell Microbiol. 2008 Apr;10(4):958-84
18031307
Mol Microbiol. 2003 Jan;47(1):103-18
12492857
Mol Microbiol. 2005 Apr;56(1):163-74
15773987
Res Microbiol. 2006 Jun;157(5):445-55
16483748
Mol Microbiol. 2008 Aug;69(4):1008-17
18627458
Microbiology (Reading). 2008 Dec;154(Pt 12):3856-3876
19047753
Vet Microbiol. 2002 Dec 20;90(1-4):299-309
12414150
Microbiol Mol Biol Rev. 2006 Dec;70(4):939-1031
17158705
Curr Opin Genet Dev. 2001 Jun;11(3):293-9
11377966
Microb Pathog. 1987 Aug;3(2):129-41
2849016
Annu Rev Microbiol. 2009;63:411-29
19514853
Philos Trans R Soc Lond B Biol Sci. 2000 May 29;355(1397):551-64
10874729
Nucleic Acids Res. 2007;35(5):1432-40
17284458
Microbes Infect. 2008 Jul;10(9):1041-50
18775788
Curr Opin Microbiol. 2009 Feb;12(1):117-24
19157959
Microbes Infect. 2007 Aug;9(10):1188-95
17719818
Nat Rev Microbiol. 2008 Feb;6(2):156-61
18026122
Microbes Infect. 2005 Mar;7(3):325-34
15804490
Cell. 2007 Jun 29;129(7):1287-98
17604718
Genes Dev. 2008 Nov 1;22(21):2914-25
18981470
Curr Opin Microbiol. 2009 Oct;12(5):559-67
19744878
Immunol Cell Biol. 2007 Feb-Mar;85(2):119-29
17213832
Mol Microbiol. 2004 Apr;52(2):601-11
15066044
J Bacteriol. 2010 Apr;192(7):1774-84
20097852
BMC Genomics. 2007 Feb 26;8:60
17324287