title: Singly and doubly labeled base-discriminating fluorescent oligonucleotide probes containing oxo-pyrene chromophore.
abstract: We have developed new oxo-pyrene labeled fluorescent nucleoside, (Oxo-Py)U which showed a strong fluorescence dependency on solvent polarity at long wavelength. The designed singly and doubly (Oxo-Py)U labeled fluorescent oligonucleotide probes were found highly efficient for the discrimination of A and consecutive AA bases of target DNA opposite to the labeled base via generation of enhanced fluorescence signal.
authors.affiliation: Bioorganic Chemistry Laboratory, Department of Chemistry, Indian Institute of Technology, Guwahati 781039, Assam, India. ssbag75@iitg.ernet.in
authors.forename: Subhendu Sekhar
authors.initials: SS
authors.lastname: Bag
authors.forename: Rajen
authors.initials: R
authors.lastname: Kundu
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Saito
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011721:Pyrenes; C030984:pyrene