title: RpoS, the stress response sigma factor, plays a dual role in the regulation of Escherichia coli's error-prone DNA polymerase IV.
abstract: RpoS, Escherichia coli's general stress response sigma factor, regulates error-prone DNA polymerase IV (Pol IV) (encoded by the dinB gene). Pol IV is induced in stationary-phase cells, and thereafter, levels of the protein remain elevated for several days of continuous incubation. This induction and persistence in stationary-phase cells are dependent on RpoS. Data presented here show that this regulation is direct via the RpoS-directed transcription of the dinB gene. However, a loss of RpoS also results in a decrease in Pol IV-dependent mutation when Pol IV is overexpressed from an RpoS-independent promoter in exponentially growing cells. The loss of RpoS also increases cell sensitivity to 4-nitroquinoline-1-oxide, indicating that RpoS affects the ability of Pol IV to bypass DNA lesions. Thus, in addition to directly driving the transcription of the dinB gene in stationary-phase cells, RpoS regulates the activity of Pol IV in exponentially growing cells via a second, indirect pathway.
authors.affiliation: Department of Biology, Indiana University, 1001 East Third Street, Bloomington, IN 47405, USA.
authors.forename: Kimberly A M
authors.initials: KA
authors.lastname: Storvik
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Foster
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C094399:DinB protein, E coli; D029968:Escherichia coli Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria
references.citation: Bioinformatics. 2009 Jun 15;25(12):1564-5
references.pmid: 19369502
references.citation: EMBO J. 2000 Jun 15;19(12):3028-37
references.pmid: 10856246
references.citation: J Bacteriol. 2004 Nov;186(21):7186-95
references.pmid: 15489429
references.citation: Mol Microbiol. 2003 Oct;50(2):549-61
references.pmid: 14617178
references.citation: Genetics. 2007 Oct;177(2):723-35
references.pmid: 17720921
references.citation: Genetics. 2001 May;158(1):41-64
references.pmid: 11333217
references.citation: Genetics. 1991 Aug;128(4):695-701
references.pmid: 1916241
references.citation: Annu Rev Microbiol. 2006;60:231-53
references.pmid: 16719715
references.citation: Mol Microbiol. 2000 Oct;38(2):299-307
references.pmid: 11069656
references.citation: Mol Cell. 2001 Mar;7(3):571-9
references.pmid: 11463382
references.citation: J Bacteriol. 2004 Jul;186(14):4802-7
references.pmid: 15231812
references.citation: J Bacteriol. 1997 Mar;179(5):1550-4
references.pmid: 9045812
references.citation: J Bacteriol. 1980 Aug;143(2):971-80
references.pmid: 6162838
references.citation: J Bacteriol. 2000 Feb;182(3):842-7
references.pmid: 10633125
references.citation: Genetica. 1992;85(2):173-9
references.pmid: 1624139
references.citation: Mol Cell. 2007 Dec 28;28(6):1058-70
references.pmid: 18158902
references.citation: J Bacteriol. 2004 May;186(9):2900-5
references.pmid: 15090533
references.citation: J Bacteriol. 2006 Jul;188(13):4992-5
references.pmid: 16788208
references.citation: Proc Natl Acad Sci U S A. 2007 Oct 2;104(40):15591-8
references.pmid: 17898175
references.citation: Biochem Biophys Res Commun. 2001 Nov 9;288(4):1052-8
references.pmid: 11689018
references.citation: J Bacteriol. 2004 Dec;186(24):8499-507
references.pmid: 15576800
references.citation: Mol Microbiol. 2005 Aug;57(3):751-61
references.pmid: 16045619
references.citation: Cold Spring Harb Symp Quant Biol. 2000;65:21-9
references.pmid: 12760017
references.citation: Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13792-7
references.pmid: 9391106
references.citation: Genetics. 2007 Jul;176(3):1431-40
references.pmid: 17483416
references.citation: Proc Natl Acad Sci U S A. 1980 May;77(5):2819-23
references.pmid: 6771759
references.citation: Mutat Res. 1995 Jun;347(1):1-7
references.pmid: 7596361
references.citation: J Bacteriol. 2005 Jan;187(2):449-57
references.pmid: 15629916
references.citation: J Bacteriol. 2000 Dec;182(23):6742-50
references.pmid: 11073920
references.citation: Curr Biol. 2010 Jan 12;20(1):80-5
references.pmid: 20036541
references.citation: Gene. 1987;53(1):85-96
references.pmid: 3596251
references.citation: Mol Genet Genomics. 2001 Oct;266(2):207-15
references.pmid: 11683261
references.citation: J Bacteriol. 2005 Mar;187(5):1591-603
references.pmid: 15716429
references.citation: Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8737-41
references.pmid: 12060704
references.citation: Annu Rev Biochem. 2002;71:17-50
references.pmid: 12045089
references.citation: Nature. 2006 Jan 12;439(7073):225-8
references.pmid: 16407906
references.citation: J Biol Chem. 1996 Jan 26;271(4):1998-2004
references.pmid: 8567650
references.citation: Mol Genet Genomics. 2004 Dec;272(5):580-91
references.pmid: 15558318
references.citation: J Bacteriol. 1995 Jul;177(14):4121-30
references.pmid: 7608087
references.citation: Curr Opin Microbiol. 2002 Dec;5(6):591-5
references.pmid: 12457703
references.citation: Nature. 2000 Apr 27;404(6781):1014-8
references.pmid: 10801133