title: Cloning, characterization, and expression analysis of a CC chemokine gene from turbot (Scophthalmus maximus).
abstract: The chemokines are a superfamily of chemotactic cytokines playing an important role in leukocyte chemotaxis. Here, a turbot head kidney cDNA library was constructed in which KC70 was identified as a CC chemokine. Unknown 5' and 3' parts of the cDNA were amplified by 5' and 3' rapid amplification of cDNA ends (RACE). The complete cDNA of KC70 contains a 59-bp 5' UTR, a 336-bp ORF, and a 152-bp 3' UTR. Four exons and three introns were identified in KC70. Phylogenetic analysis showed that KC70 was similar to CCL19. In normal turbot KC70 was expressed in all tissues except brain and skin. Infection of turbot with pathogenic bacteria significantly increased expression of KC70 in the liver. Expression of KC70 in head kidney first increased and then decreased after bacterial challenge. No significant change was observed in the spleen after bacterial challenge. During embryonic development, KC70 was highly expressed after the gastrula stage. These results indicated KC70 plays important and multiple roles in turbot immune response.
authors.affiliation: Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Nanjing Road 106, Qingdao 266071, China. chensl@ysfri.ac.cn
authors.forename: S L
authors.initials: SL
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: X L
authors.initials: XL
authors.lastname: Dong
authors.forename: L
authors.initials: L
authors.lastname: Meng
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019742:Chemokines, CC / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D029941:Fish Proteins / Q000235:genetics*; D005412:Flatfishes / Q000196:embryology / Q000235:genetics* / Q000276:immunology*; D018507:Gene Expression Regulation, Developmental; D007113:Immunity, Innate / Q000235:genetics; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D014018:Tissue Distribution
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D019742:Chemokines, CC; D017931:DNA Primers; D018076:DNA, Complementary; D029941:Fish Proteins
references.citation: Immunogenetics. 2005 Jul;57(6):409-19
references.pmid: 16001127
references.citation: Genomics. 2006 Sep;88(3):381-5
references.pmid: 16697551
references.citation: Immunogenetics. 1999 Sep;49(10):909-14
references.pmid: 10436187
references.citation: Immunol Rev. 1998 Dec;166:341-8
references.pmid: 9914924
references.citation: Dev Comp Immunol. 2001 Oct-Dec;25(8-9):827-39
references.pmid: 11602198
references.citation: Immunogenetics. 2007 Aug;59(8):613-23
references.pmid: 17541578
references.citation: Immunogenetics. 2003 Mar;54(12):884-95
references.pmid: 12671740
references.citation: Fish Shellfish Immunol. 2007 Mar;22(3):172-81
references.pmid: 16908195
references.citation: Fish Shellfish Immunol. 2006 Feb;20(2):137-51
references.pmid: 15950491
references.citation: Mol Genet Genomics. 2006 Mar;275(3):297-309
references.pmid: 16341548
references.citation: Immunogenetics. 2004 Feb;55(11):763-9
references.pmid: 14722688
references.citation: Nature. 1982 Mar 25;296(5855):311-3
references.pmid: 7063031
references.citation: Nature. 2002 Dec 5;420(6915):520-62
references.pmid: 12466850
references.citation: J Immunol. 2006 Jan 1;176(1):401-15
references.pmid: 16365434
references.citation: Gene. 2001 Feb 21;264(2):197-203
references.pmid: 11250074
references.citation: Dev Comp Immunol. 2004 May 3;28(5):443-60
references.pmid: 15062643
references.citation: Mol Immunol. 2004 Jul;41(8):793-808
references.pmid: 15234559
references.citation: Fish Shellfish Immunol. 2007 Oct;23(4):711-20
references.pmid: 17604647
references.citation: Brief Bioinform. 2004 Jun;5(2):150-63
references.pmid: 15260895
references.citation: Protein Eng. 1997 Jan;10(1):1-6
references.pmid: 9051728
references.citation: Immunogenetics. 2004 Aug;56(5):379-87
references.pmid: 15309344
references.citation: Dev Comp Immunol. 2006;30(9):783-96
references.pmid: 16510183