Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43.
Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.
Lee
Hyun Kyoung
HK
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Lee
Byoung Hee
BH
Seok
Seung Hyeok
SH
Baek
Min Won
MW
Lee
Hui Young
HY
Kim
Dong Jae
DJ
Na
Yi Rang
YR
Noh
Kyoung Jin
KJ
Park
Sung Hoon
SH
Kumar
Dutta Noton
DN
Kariwa
Hiroaki
H
Nakauchi
Mina
M
Heo
Suk Jin
SJ
Park
Jae Hak
JH
D000914:Antibodies, Viral / Q000276:immunology*; D015153:Blotting, Western; D028941:Coronavirus 229E, Human / Q000276:immunology*; D028962:Coronavirus OC43, Human / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis / Q000276:immunology*
D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins
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