title: Use of a hand-held meter for detecting subclinical ketosis in dairy cows.
abstract: The Optium Xceed is a new hand-held meter for determining blood β-hydroxybutyrate (BHBA) and glucose in human medicine. The objective of this study was to compare BHBA and glucose results obtained using the hand-held meter with those results made with a laboratory method and to evaluate its usefulness as a cowside test in the diagnosis of subclinical ketosis (SCK) in dairy cows. Seventy-eight blood samples from clinically healthy Holstein cows between 5 and 60 days post-calving were analysed. BHBA and glucose values were significantly higher with the hand-held meter versus laboratory methods. Correlation coefficients (r) for BHBA and glucose with the Optium Xceed versus laboratory methods were 0.97 and 0.63, respectively. Based on Bland-Altman plot and Passing-Bablok regression, agreement between two methods was good for BHBA but the agreement for glucose was only fair. When SCK was defined as plasma BHBA levels ≥ 1200 μmol/L, the sensitivity and specificity of the hand-held meter ketone testing in determining SCK were 85% and 94%, respectively. Raising the threshold of the laboratory method to ≥ 1400 μmol/L, the sensitivity and specificity incremented to 0.90 and 0.98, respectively. In conclusion, the blood ketone-monitoring device can be used as a rapid and reliable diagnostic test to detect SCK under field conditions.
authors.affiliation: Department of Internal Medicine, Faculty of Veterinary Medicine, Adnan Menderes University, PK 17, 09016 Aydin, Turkey. hvoyvoda@adu.edu.tr
authors.forename: Huseyin
authors.initials: H
authors.lastname: Voyvoda
authors.forename: Hasan
authors.initials: H
authors.lastname: Erdogan
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000175:diagnosis; D019411:Clinical Laboratory Techniques / Q000295:instrumentation / Q000662:veterinary; D005260:Female; D007662:Ketosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity; D014730:Veterinary Medicine / Q000295:instrumentation / Q000379:methods
chemical_list: D001786:Blood Glucose; D020155:3-Hydroxybutyric Acid