title: The genetic basis of laboratory adaptation in Caulobacter crescentus.
abstract: The dimorphic bacterium Caulobacter crescentus has evolved marked phenotypic changes during its 50-year history of culture in the laboratory environment, providing an excellent system for the study of natural selection and phenotypic microevolution in prokaryotes. Combining whole-genome sequencing with classical molecular genetic tools, we have comprehensively mapped a set of polymorphisms underlying multiple derived phenotypes, several of which arose independently in separate strain lineages. The genetic basis of phenotypic differences in growth rate, mucoidy, adhesion, sedimentation, phage susceptibility, and stationary-phase survival between C. crescentus strain CB15 and its derivative NA1000 is determined by coding, regulatory, and insertion/deletion polymorphisms at five chromosomal loci. This study evidences multiple genetic mechanisms of bacterial evolution as driven by selection for growth and survival in a new selective environment and identifies a common polymorphic locus, zwf, between lab-adapted C. crescentus and clinical isolates of Pseudomonas aeruginosa that have adapted to a human host during chronic infection.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.
authors.forename: Melissa E
authors.initials: ME
authors.lastname: Marks
authors.forename: Cyd Marie
authors.initials: CM
authors.lastname: Castro-Rojas
authors.forename: Clotilde
authors.initials: C
authors.lastname: Teiling
authors.forename: Lei
authors.initials: L
authors.lastname: Du
authors.forename: Vinayak
authors.initials: V
authors.lastname: Kapatral
authors.forename: Theresa L
authors.initials: TL
authors.lastname: Walunas
authors.forename: Sean
authors.initials: S
authors.lastname: Crosson
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001435:Bacteriophages / Q000502:physiology; D016935:Caulobacter crescentus / Q000235:genetics* / Q000378:metabolism* / Q000821:virology; D019143:Evolution, Molecular; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D001426:Bacterial Proteins
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