title: Vif of feline immunodeficiency virus from domestic cats protects against APOBEC3 restriction factors from many felids.
abstract: To get more insight into the role of APOBEC3 (A3) cytidine deaminases in the species-specific restriction of feline immunodeficiency virus (FIV) of the domestic cat, we tested the A3 proteins present in big cats (puma, lion, tiger, and lynx). These A3 proteins were analyzed for expression and sensitivity to the Vif protein of FIV. While A3Z3s and A3Z2-Z3s inhibited Deltavif FIV, felid A3Z2s did not show any antiviral activity against Deltavif FIV or wild-type (wt) FIV. All felid A3Z3s and A3Z2-Z3s were sensitive to Vif of the domestic cat FIV. Vif also induced depletion of felid A3Z2s. Tiger A3s showed a moderate degree of resistance against the Vif-mediated counter defense. These findings may imply that the A3 restriction system does not play a major role to prevent domestic cat FIV transmission to other Felidae. In contrast to the sensitive felid A3s, many nonfelid A3s actively restricted wt FIV replication. To test whether Vif(FIV) can protect also the distantly related human immunodeficiency virus type 1 (HIV-1), a chimeric HIV-1.Vif(FIV) was constructed. This HIV-1.Vif(FIV) was replication competent in nonpermissive feline cells expressing human CD4/CCR5 that did not support the replication of wt HIV-1. We conclude that the replication of HIV-1 in some feline cells is inhibited only by feline A3 restriction factors and the absence of the appropriate receptor or coreceptor.
authors.affiliation: Clinic for Gastroenterology, Hepatology and Infectiology, Heinrich-Heine-University, Building 23.12.U1.87, Moorenstr. 5, 40225 Düsseldorf, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Zielonka
authors.forename: Daniela
authors.initials: D
authors.lastname: Marino
authors.forename: Henning
authors.initials: H
authors.lastname: Hofmann
authors.forename: Naoya
authors.initials: N
authors.lastname: Yuhki
authors.forename: Martin
authors.initials: M
authors.lastname: Löchelt
authors.forename: Carsten
authors.initials: C
authors.lastname: Münk
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D043525:Cytosine Deaminase / Q000235:genetics / Q000378:metabolism*; D045989:Felidae / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D016181:Feline Acquired Immunodeficiency Syndrome / Q000821:virology*; D016346:Gene Products, vif / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D012326:RNA Splicing; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D016346:Gene Products, vif; D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; D043525:Cytosine Deaminase
references.citation: Virology. 1995 Feb 1;206(2):935-44
references.pmid: 7531918
references.citation: J Gen Virol. 2007 Oct;88(Pt 10):2793-2799
references.pmid: 17872533
references.citation: Virology. 1994 Nov 1;204(2):860-7
references.pmid: 7941360
references.citation: J Virol. 2007 Oct;81(20):10961-9
references.pmid: 17670835
references.citation: Curr Biol. 2005 Jan 26;15(2):166-70
references.pmid: 15668174
references.citation: Nat Med. 2003 Nov;9(11):1398-403
references.pmid: 14528301
references.citation: Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3770-4
references.pmid: 14999100
references.citation: J Virol. 2007 Apr;81(8):3807-15
references.pmid: 17267497
references.citation: Genes Dev. 2004 Dec 1;18(23):2867-72
references.pmid: 15574593
references.citation: J Biol Chem. 2006 Aug 4;281(31):22161-22172
references.pmid: 16735504
references.citation: Trends Biochem Sci. 2007 Mar;32(3):118-28
references.pmid: 17303427
references.citation: J Virol. 2005 Jul;79(13):8282-94
references.pmid: 15956574
references.citation: J Virol. 2007 Dec;81(24):13932-7
references.pmid: 17942564
references.citation: Cell Host Microbe. 2008 Sep 11;4(3):249-59
references.pmid: 18779051
references.citation: Nat Med. 2003 Nov;9(11):1404-7
references.pmid: 14528300
references.citation: Nucleic Acids Res. 2006;34(19):5683-94
references.pmid: 17038330
references.citation: Mol Ther. 2000 Jan;1(1):31-8
references.pmid: 10933909
references.citation: J Virol. 2009 Feb;83(4):1992-2003
references.pmid: 19036809
references.citation: Genome Biol. 2008;9(3):R48
references.pmid: 18315870
references.citation: J Virol. 2009 Jan;83(2):494-7
references.pmid: 18987154
references.citation: Clin Microbiol Rev. 2006 Oct;19(4):728-62
references.pmid: 17041142
references.citation: J Virol. 2006 Mar;80(6):2728-37
references.pmid: 16501082
references.citation: J Virol. 2009 Apr;83(8):3486-95
references.pmid: 19153238
references.citation: J Virol. 1996 Oct;70(10):6682-93
references.pmid: 8794304
references.citation: J Virol. 2007 Jul;81(13):7099-110
references.pmid: 17428871
references.citation: J Virol. 2006 Nov;80(21):10522-33
references.pmid: 16920826
references.citation: Vet Immunol Immunopathol. 2008 May 15;123(1-2):32-44
references.pmid: 18359092
references.citation: J Virol. 2009 Jan;83(1):295-303
references.pmid: 18945781
references.citation: J Biol Chem. 2004 Apr 9;279(15):14481-3
references.pmid: 14966139
references.citation: J Virol. 2007 Jul;81(13):7048-60
references.pmid: 17459941
references.citation: Virology. 2007 Apr 25;361(1):112-22
references.pmid: 17169394
references.citation: J Virol. 1990 Oct;64(10):4605-13
references.pmid: 1697907
references.citation: J Virol. 2009 Aug;83(16):8270-5
references.pmid: 19494015
references.citation: Annu Rev Immunol. 2008;26:317-53
references.pmid: 18304004
references.citation: J Virol. 2006 Sep;80(17):8450-8
references.pmid: 16912295
references.citation: J Virol. 2004 Oct;78(20):11070-6
references.pmid: 15452227
references.citation: Proc Natl Acad Sci U S A. 2005 May 31;102(22):7982-7
references.pmid: 15911774
references.citation: Virology. 1997 Jun 23;233(1):185-92
references.pmid: 9201228
references.citation: Proc Natl Acad Sci U S A. 2004 Apr 13;101(15):5652-7
references.pmid: 15054139
references.citation: Proc Natl Acad Sci U S A. 1989 Aug;86(15):5743-7
references.pmid: 2762293
references.citation: J Virol. 2008 Dec;82(23):11889-901
references.pmid: 18818324
references.citation: Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3927-32
references.pmid: 14978281
references.citation: Vet Immunol Immunopathol. 2010 Mar 15;134(1-2):25-32
references.pmid: 19896218
references.citation: J Biol Chem. 2007 Jan 26;282(4):2587-95
references.pmid: 17121840
references.citation: Virology. 2008 Jan 5;370(1):113-21
references.pmid: 17916373
references.citation: Science. 2003 Nov 7;302(5647):1056-60
references.pmid: 14564014
references.citation: Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6261-5
references.pmid: 17384150
references.citation: Cell. 2003 Jul 11;114(1):21-31
references.pmid: 12859895
references.citation: PLoS One. 2008;3(12):e3963
references.pmid: 19088851
references.citation: Vet Immunol Immunopathol. 2010 Mar 15;134(1-2):14-24
references.pmid: 19896724
references.citation: J Virol. 2009 Aug;83(15):7547-59
references.pmid: 19458006
references.citation: J Wildl Dis. 2007 Oct;43(4):700-10
references.pmid: 17984266
references.citation: Nucleic Acids Res. 2007;35(21):7096-108
references.pmid: 17942420
references.citation: J Virol. 1999 Sep;73(9):7916-21
references.pmid: 10438892
references.citation: J Virol. 2001 Apr;75(7):3141-51
references.pmid: 11238841
references.citation: J Virol. 1998 Jun;72(6):5085-92
references.pmid: 9573279
references.citation: Science. 1992 Dec 18;258(5090):1938-41
references.pmid: 1470917
references.citation: Methods Mol Biol. 2003;229:251-71
references.pmid: 12824636