Investigation of the dynamical properties of water in elastin by deuterium Double Quantum Filtered NMR.
The anisotropic motion of tightly bound waters of hydration in bovine nuchal ligament elastin has been studied by deuterium Double Quantum Filtered (DQF) NMR. The experiments have allowed for a direct measurement of the degree of anisotropy within pores of elastin over a time scale ranging from 100 micros to 30 ms, corresponding to a tortuous spatial displacement ranging from 0.2 to 7 microm. We studied the anisotropic motion of deuterium nuclei in D2O hydrated elastin over a temperature of -15 degrees C to 37 degrees C and in solvents with varying dielectric constants. Our experimental measurements of the residual quadrupolar interaction as a function of temperature are correlated to the existing notion of hydrophobic collapse near 20 degrees C.
Sun
Cheng
C
Brooklyn College, Department of Physics, 2900 Bedford Avenue Brooklyn, NY 11210, United States.
Boutis
Gregory S
GS
D000465:Algorithms; D000818:Animals; D016880:Anisotropy; D002417:Cattle; D003903:Deuterium / Q000737:chemistry*; D004549:Elastin / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011789:Quantum Theory; D013696:Temperature; D014867:Water / Q000737:chemistry*
D014867:Water; D004549:Elastin; D003903:Deuterium
Biochemistry. 1975 Nov 18;14(23):5175-83
1191633
Biopolymers. 1974 Apr;13(4):677-86
4847581
Science. 1990 Oct 12;250(4978):297-8
2218535
Biochem Biophys Res Commun. 1985 Jul 16;130(1):50-7
4026843
J Phys Chem. 1971 Jan 7;75(1):142-9
5167451
Biopolymers. 1985 Dec;24(12):2345-56
4092092
Biopolymers. 1986;25 Suppl:S209-28
3779026
Magn Reson Med. 2007 Jun;57(6):990-9
17534920
NMR Biomed. 2001 Apr;14(2):112-32
11320537
Biopolymers. 1976 May;15(5):813-32
1260105
Nature. 1973 Dec 21-28;246(5434):461-6
4762195
Biopolymers. 2007 Dec 5-15;87(5-6):352-9
17806105
Biopolymers. 1991 Apr;31(5):465-75
1868163
J Mol Biol. 2001 Jan 19;305(3):581-92
11152614
Biophys J. 2009 Jul 8;97(1):312-20
19580769
J Mol Biol. 1998;283(1):255-64
9761688
Nature. 1970 Aug 15;227(5259):718-21
5432073
Biochem Biophys Res Commun. 1986 Dec 15;141(2):749-55
3801025
J Protein Chem. 1988 Feb;7(1):1-34
3076447
Ultrastruct Pathol. 1983 Mar-Apr;4(2-3):227-51
6683883
J Magn Reson. 1997 Nov;129(1):98-104
9405221
CRC Crit Rev Biochem. 1976 Jun;4(1):1-45
782788
Biochem J. 1955 Sep;61(1):11-21
13260170
Biorheology. 1993 May-Aug;30(3-4):229-42
7506945