Production of glutaminase(E.C. 3.2.1.5) from Zygosaccharomyces rouxii in solid-state fermentation and modeling the growth of Z. rouxii therein.
Glutaminase production in Zygosacchromyces rouxii by solid-state fermentation (SSF) is detailed. Substrates screening showed best results with oatmeal (OM) and wheatbran (WB). Further, a 1:1 combination of OM: WB gave 0.614units/gds with artificial sea water (ASW) as moistening agent. Evaluation of additional carbon, nitrogen, aminoacids and minerals supplementation was done. A central composite design was employed to investigate effects of four variables, viz. moisture content, glucose, corn steep liquor and glutamine on production. A 4-fold increase in enzyme production was obtained. Studies were undertaken to analyze the time course model the microbial growth and nutrient utilization during SSF. Logistic equation (R2=0.8973), describing the growth model of Z.rouxii was obtained, with maximum values of micronm and Xm at 0.326h-1 and 7.35% of dry matter weight loss, respectively. A good-fit model to describe utilization of total carbohydrate (R2=0.9906) nitrogen concentration (R2=0.9869) with time was obtained. The model was used successfully to predict enzyme production (R2=0.7950).
Iyer
Padma
P
Food Engeering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai, India. iyer_padma@rediffmail.com
Singhal
Rekha S
RS
D005285:Fermentation; D005972:Glutaminase / Q000096:biosynthesis* / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D008954:Models, Biological; D015233:Models, Statistical; D020068:Zygosaccharomyces / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism
D005972:Glutaminase