Comparative transcriptomics and proteomics of p-hydroxybenzoate producing Pseudomonas putida S12: novel responses and implications for strain improvement.
A transcriptomics and proteomics approach was employed to study the expression changes associated with p-hydroxybenzoate production by the engineered Pseudomonas putida strain S12palB1. To establish p-hydroxybenzoate production, phenylalanine-tyrosine ammonia lyase (pal/tal) was introduced to connect the tyrosine biosynthetic and p-coumarate degradation pathways. In agreement with the efficient p-hydroxybenzoate production, the tyrosine biosynthetic and p-coumarate catabolic pathways were upregulated. Also many transporters were differentially expressed, one of which--a previously uncharacterized multidrug efflux transporter with locus tags PP1271-PP1273--was found to be associated with p-hydroxybenzoate export. In addition to tyrosine biosynthesis, also tyrosine degradative pathways were upregulated. Eliminating the most prominent of these resulted in a 22% p-hydroxybenzoate yield improvement. Remarkably, the upregulation of genes contributing to p-hydroxybenzoate formation was much higher in glucose than in glycerol-cultured cells.
Verhoef
Suzanne
S
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628, BC, Delft, the Netherlands.
Ballerstedt
Hendrik
H
Volkers
Rita J M
RJ
de Winde
Johannes H
JH
Ruijssenaars
Harald J
HJ
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D010226:Parabens / Q000378:metabolism*; D040901:Proteomics; D016958:Pseudomonas putida / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D010226:Parabens; C038193:4-hydroxybenzoic acid
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