title: [Effects of differentially expressed proteins in hepatocellular carcinoma cell treated by different telomerase inhibitors].
abstract: OBJECTIVE
  To detect differentially expressed proteins in hepatocellular carcinoma cell line SMMC-7721 treated separately by eight telomerase inhibitors including antisense oligodeoxynuclectide of human telomerase RNA (hTR-ASODN), sense oligodeoxynuclectide of hTR (hTR-SODN), ASODN of human telomerase reverse transcriptase (hTERT-ASODN), SODN of hTERT (hTERT-SODN), epigallocatechin gallate (EGCG), 3'-azido-3'-deoxythymidine (AZT), all trans-retinoic acid (ATRA) and adriamycin (ADM) using surface enhanced laser desorption/ionization time of flight-mass spectrom (SELDI-TOF-MS) technology.
  
  
  METHODS
  SELDI-TOF-MS technology and weak cation exchanger (WCX-2) protein chip were used to detect differentially expressed secretory and cytoplasmic proteins of SMMC-7721 cell treated separately by eight telomerase inhibitors. The control group was hepatocellular carcinoma SMMC-7721 cell without any disposal.
  
  
  RESULTS
  The results of WCX-2 protein chip showed that the secretory and cytoplasmic proteins were differentially expressed in SMMC-7721 cell treated separately by eight telomerase inhibitors. But some proteins were down-regulated or up-regulated together in all experimental groups. The molecular weight of these differential proteins were all less than 10,000 Da.
  
  
  CONCLUSIONS
  Differentially expressed and common changes of proteins in SMMC-7721 cell treated separately by eight telomerase inhibitors would associate with telomerase activity.
authors.affiliation: Key Laboratory of Public Health and Safety,Ministry of Education, School of Public Health, Fudan University, Shanghai 200032, China. wx_smile@126.com
authors.forename: Xiao
authors.initials: X
authors.lastname: Wei
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zheng
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis*; D019098:Telomerase / Q000037:antagonists & inhibitors*; D014212:Tretinoin / Q000494:pharmacology; D015215:Zidovudine / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D020543:Proteome; D015215:Zidovudine; D014212:Tretinoin; D002392:Catechin; C045651:epigallocatechin gallate; D019098:Telomerase