title: Impact of reattaching various Aeromonas salmonicida A-layer proteins on vaccine efficacy in Atlantic cod (Gadus morhua).
abstract: Atypical Aeromonas salmonicida causes atypical furunculosis in a whole range of farmed fish species. The bacterium comprises a heterogeneous group differing in surface components such as the A-layer protein and O-chain polysaccharide structures. Previously, the A-layer protein was shown to contribute to protective immunity as a vaccine with A. salmonicida cells with reattached A-layer protein protecting significantly better than the corresponding A-layer deficient bacteria used in the Atlantic cod (Gadus morhua) vaccine. In the present study, genetically different A. salmonicida A-proteins, either as preformed A-layer sheets from culture supernatants or as purified preparations, were shown to attach to A-layer deficient isolates with a different O-chain structure. Only vaccines containing A. salmonicida cells with reattached A-protein genetically homologous to the challenge isolate, elicited protection comparable to that of the homologous vaccine.
authors.affiliation: Nofima Marin, P.O. Box 6122, N-9291 Tromsø, Norway. kathrine.ryvold.arnesen@nofima.no
authors.forename: Kathrine R
authors.initials: KR
authors.lastname: Arnesen
authors.forename: Helene
authors.initials: H
authors.lastname: Mikkelsen
authors.forename: Merete B
authors.initials: MB
authors.lastname: Schrøder
authors.forename: Vera
authors.initials: V
authors.lastname: Lund
mesh_terms: D048409:Aeromonas salmonicida / Q000737:chemistry* / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D002626:Chemistry, Pharmaceutical; D048389:Gadus morhua / Q000276:immunology*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology* / Q000517:prevention & control / Q000662:veterinary*; D007274:Injections, Intraperitoneal; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology; D014611:Vaccination / Q000379:methods / Q000662:veterinary
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D011134:Polysaccharides