[DNA damage of mice endometrial cells at implantation phase exposed to carbon disulfide].
OBJECTIVE
To detect the DNA damage of mice endometrial cells induced by carbon disulfide with single cell gel electrophoresis (SCGE) and explore the mechanism of embryo implantation disorder.


METHODS
Endometrial cells, obtained by mechanical scrape, were used to test cell viability by trypan blue. Single cell suspension was exposed to the different concentrations of carbon disulfide (CS(2)) at four dose groups (0, 500, 1000, 2500 micromol/L). DNA damage was detected by SCGE. The SCGE results were analyzed by CASP software.


RESULTS
Different dosages of CS(2) concentration induced different varying degrees of damage, forming typical normal cell and comet cell images. Compared with the control group, HDNA% decreased by 7.49%, 12.19% and 24.36% respectively, and TDNA%, TL, OTM increased by 7.13, 11.60, 23.18, 3.68, 5.98, 9.62, and 9.16, 16.84, 39.32 times respectively, in the groups of 500, 1000, 2500 micromol/L CS(2) (P < 0.01). Compared with the group of 500, 1000 micromol/L CS(2), TDNA%, TL, CL, TM, OTM in the group of 2500 micromol/L CS(2) increased by 1.98, 0.92, 1.27, 0.52, 0.37 and 0.17, 5.31, 1.90, 2.97, 1.26 times respectively(P < 0.01). Compared with the group of 500 micromol/L CS(2), TDNA%, TL, CL, TM, OTM in the group of 1000 micromol/L CS(2) increased by 0.55, 0.49, 0.16, 1.18, 0.76 times respectively (P < 0.01). The result of regression analysis showed that regression coefficients between HDNA%, TDNA%, TL, TM, OTM and the doses were -13.78, 13.78, 0.05, 4.38 and 3.23 respectively.


CONCLUSIONS
CS(2) exposure could induce DNA damage in the endometrial cells of mice at the phase of implantation. The degree of DNA damage increases with the increasing CS(2) concentration. CS(2) might affect the implantation of embryo by doing harm to the endometrial cells.
Shen
Chun-Zi
CZ
Institute of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan 250012, China.
Wang
Zhi-Ping
ZP
Sun
Shu-Ai
SA
D000818:Animals; D002246:Carbon Disulfide / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D010064:Embryo Implantation / Q000187:drug effects*; D004717:Endometrium / Q000166:cytology / Q000187:drug effects*; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains
D002246:Carbon Disulfide