Identification of uricase as a potential target of plant thioredoxin: Implication in the regulation of nodule development.
During symbiotic nodule development in legume roots, early signaling events between host and rhizobia serve critical determinants for the proper onset of nodule morphogenesis, nitrogen fixation, and assimilation. Previously we isolated thioredoxin from soybean nodules as one of differentially expressed genes during nodulation and noted its positive role in nitrogen fixation. To identify the target proteins of thioredoxin in nodules, we used thioredoxin affinity chromatography followed by mass spectrometry. Nodulin-35, a subunit of uricase, was found to be a target of thioredoxin. Their interaction was confirmed by pull-down assay and by bimolecular fluorescent complementation. With an increased uricase activity observed also in the presence of thioredoxin, these results appear to implicate a novel role of thioredoxin in the regulation of enzyme activities involved in nodule development and nitrogen fixation.
Du
Hui
H
Department of Biological Science, Sookmyung Women's University, Seoul, Republic of Korea.
Kim
Sunghan
S
Nam
Kyoung Hee
KH
Lee
Myung-Sok
MS
Son
Ora
O
Lee
Suk-Ha
SH
Cheon
Choong-Ill
CI
D008565:Membrane Proteins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D053204:Root Nodules, Plant / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology; D013025:Glycine max / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology; D013559:Symbiosis; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*
D008565:Membrane Proteins; D010940:Plant Proteins; C045625:nodulin; D013879:Thioredoxins