title: Structural analysis and biomedical applications of dextran produced by a new isolate Pediococcus pentosaceus screened from biodiversity hot spot Assam.
abstract: Dextran produced by a natural isolate of Pediococcus pentosaceus, screened from Assam, in the Northeastern region of India, was estimated, purified, structure characterised and functionality analysed. The dextran concentration in the cell free supernatant of the isolate P. pentosaceus was 10.2mg/ml. FT-IR analysis revealed the hydroxyl and carboxyl functional groups present in the dextran. (1)H NMR and (13)C NMR spectral data revealed that the dextran has a linear backbone of alpha-(1-->6) linked D-glucose residues. The decrease in viscosity of dextran solution with the increase in shear rate, threw light on its typical non-Newtonian pseudoplastic behaviour. The cytotoxicity tests on human cervical cancer (HeLa) cell line was studied which showed the dextran is non-toxic and biocompatible, rendering it safe for drug delivery, tissue engineering and various other biomedical applications.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
authors.forename: Seema
authors.initials: S
authors.lastname: Patel
authors.forename: Naresh
authors.initials: N
authors.lastname: Kasoju
authors.forename: Utpal
authors.initials: U
authors.lastname: Bora
authors.forename: Arun
authors.initials: A
authors.lastname: Goyal
mesh_terms: D044822:Biodiversity; D003911:Dextrans / Q000096:biosynthesis* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010376:Pediococcus / Q000378:metabolism*; D012212:Rheology; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003911:Dextrans