title: BCG vaccination in the cotton rat (Sigmodon hispidus) infected by the pulmonary route with virulent Mycobacterium tuberculosis.
abstract: To evaluate the usefulness of the American cotton rat (Sigmodon hispidus) in the evaluation of vaccine-induced resistance, we infected BCG-vaccinated and non-vaccinated cotton rats with Mycobacterium tuberculosis (H37Rv) via the respiratory route. Lung histopathology of these animals showed loose, disorganized granulomas which were non-necrotic up to 8 weeks post-infection. Moreover, we were not able to detect a DTH response after intradermal injection with PPD antigen. Prior BCG vaccination significantly reduced lung and spleen bacterial loads by 1-1.5log CFU and upregulated PPD-induced proliferation and production of IFNgamma in lymphocyte cultures. We conclude that pulmonary infection of the cotton rat with Mtb more closely resembles the phenotype seen in mice rather than guinea pigs.
authors.affiliation: Department of Microbial and Molecular Pathogenesis, Texas A&M Health Science Center, College Station, TX 77843-1114, USA.
authors.forename: Christine T
authors.initials: CT
authors.lastname: McFarland
authors.forename: Lan
authors.initials: L
authors.lastname: Ly
authors.forename: Amminikutty
authors.initials: A
authors.lastname: Jeevan
authors.forename: Toshiko
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Bradley
authors.initials: B
authors.lastname: Weeks
authors.forename: Angelo
authors.initials: A
authors.lastname: Izzo
authors.forename: David
authors.initials: D
authors.lastname: McMurray
mesh_terms: D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage* / Q000276:immunology; D058491:Bacterial Load; D049109:Cell Proliferation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D006099:Granuloma / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000096:biosynthesis; D008168:Lung / Q000382:microbiology; D008297:Male; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity*; D016205:Sigmodontinae; D013154:Spleen / Q000276:immunology / Q000382:microbiology; D014373:Tuberculin / Q000276:immunology; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D014611:Vaccination / Q000379:methods; D014774:Virulence
chemical_list: D000336:Aerosols; D001500:BCG Vaccine; D014373:Tuberculin; D007371:Interferon-gamma
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