title: Structural disorder in proteins of the rhabdoviridae replication complex.
abstract: Rhabdoviridae are single stranded negative sense RNA viruses. The viral RNA condensed by the nucleoprotein (N), the phosphoprotein (P) and the large subunit (L) of the RNA-dependent RNA polymerase are the viral components of the transcription/replication machineries. Both P and N contain intrinsically disordered regions (IDRs) that play different roles in the virus life cycle. Here, we describe the modular organization of P based on recent structural, biophysical and bioinformatics data. We show how flexible loops in N participate in the attachment of P to the N-RNA template by an induced-fit mechanism. Finally, we discuss the roles of IDRs in the mechanism of replication/transcription, and propose a new model for the interaction of the L subunit with its N-RNA template.
authors.affiliation: Unit of Virus Host Cell Interactions, UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Leyrat
authors.forename: Francine C A
authors.initials: FC
authors.lastname: Gérard
authors.forename: Euripedes
authors.initials: E
authors.lastname: de Almeida Ribeiro
authors.forename: Ivan
authors.initials: I
authors.lastname: Ivanov
authors.forename: Rob W H
authors.initials: RW
authors.lastname: Ruigrok
authors.forename: Marc
authors.initials: M
authors.lastname: Jamin
mesh_terms: D016679:Genome, Viral; D008958:Models, Molecular; D009698:Nucleoproteins / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D012209:Rhabdoviridae / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D009698:Nucleoproteins; D010750:Phosphoproteins