Detection of pathogenic Streptococcus suis bacteria using magnetic glycoparticles.
Detection of the zoonotic bacterial pathogen Streptococcus suis was achieved using magnetic glycoparticles. The bacteria contain an adhesion protein for the carbohydrate sequence Galalpha1,4Gal. After incubation with various amounts of the pathogen, magnetic concentration and ATP detection, bacterial levels down to 10(5) cfu could be detected. Submicrometer particles were needed, since with the larger microparticles the method did not succeed.
Pera
Núria Parera
NP
Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands.
Kouki
Annika
A
Haataja
Sauli
S
Branderhorst
Hilbert M
HM
Liskamp
Rob M J
RM
Visser
Gerben M
GM
Finne
Jukka
J
Pieters
Roland J
RJ
D000255:Adenosine Triphosphate / Q000032:analysis; D001426:Bacterial Proteins / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D008163:Luminescent Measurements; D008280:Magnetics; D010316:Particle Size; D017011:Streptococcus suis / Q000302:isolation & purification* / Q000378:metabolism
D001426:Bacterial Proteins; D002241:Carbohydrates; D000255:Adenosine Triphosphate