Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays.
We demonstrate the feasibility of a nanopore based single-molecule DNA sequencing method, which employs multicolor readout. Target DNA is converted according to a binary code, which is recognized by molecular beacons with two types of fluorophores. Solid-state nanopores are then used to sequentially strip off the beacons, leading to a series of detectable photon bursts, at high speed. We show that signals from multiple nanopores can be detected simultaneously, allowing straightforward parallelization to large nanopore arrays.
McNally
Ben
B
Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA.
Singer
Alon
A
Yu
Zhiliang
Z
Sun
Yingjie
Y
Weng
Zhiping
Z
Meller
Amit
A
D004270:DNA, Circular / Q000737:chemistry*; D036103:Nanotechnology; D017422:Sequence Analysis, DNA / Q000379:methods*
D004270:DNA, Circular
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