title: Assessment of the chemical changes induced in human melanoma cells by boric acid treatment using infrared imaging.
abstract: Boron is found in everyday foods and drinking water in trace quantities. Boron exists as boric acid (BA) within plants and animals, where low levels have been linked to cancer incidence. However, this correlation is not well characterized. In this study, we examined the chemical and morphological effects of BA on human skin melanoma cells (SK-MEL28) using Fourier Transform InfraRed Imaging (FTIRI) with a Focal Plane Array (FPA) detector. Cells were grown under concentrations of BA ranging from 0 to 50 mM. Cell viability was determined after 1, 2, 3, 5, 7 and 10 days using trypan blue staining. With FTIRI, images of approximately twenty cells per time point per condition were collected. Principal components analysis (PCA) was used to evaluate changes in cell composition, with particular focus on the lipid, protein, and nucleic acid spectral components. Results from trypan blue staining revealed decreased cell viability as BA concentration increased. FTIRI data indicated that the protein and lipid contents (as indicated by the lipid/protein ratio) did not undergo substantial changes due to BA treatment. In contrast, the nucleic acid/protein ratio significantly decreased with BA treatment. PCA results showed an increase in beta-sheet protein at higher concentrations of BA (12.5, 25, and 50 mM). Together, these results suggest that high concentrations of BA have an anti-proliferative effect and show signs consistent with apoptosis.
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794, USA.
authors.forename: Alvin S
authors.initials: AS
authors.lastname: Acerbo
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Miller
mesh_terms: D001888:Boric Acids / Q000494:pharmacology*; D003952:Diagnostic Imaging; D006801:Humans; D007259:Infrared Rays; D008545:Melanoma / Q000737:chemistry / Q000378:metabolism*; D009696:Nucleic Acids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012878:Skin Neoplasms / Q000473:pathology*; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014018:Tissue Distribution / Q000187:drug effects
chemical_list: D001888:Boric Acids; D009696:Nucleic Acids; D011506:Proteins; C032688:boric acid