title: A simple and fast method to analyze the orientation of c-type cytochromes in the outer membrane of Gram-negative bacteria.
abstract: Outer membrane cytochromes catalyze the final reduction step of respiratory chains to electron acceptors that cannot diffuse through the outer membrane of Gram-negative bacteria. We developed an in vivo method to detect the orientation of outer membrane cytochromes via analysis of electron transfer reactions between these enzymes and riboflavin.
authors.affiliation: Department of Microbiology, University of Freiburg, D-79104 Freiburg, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Richter
authors.forename: Clemens
authors.initials: C
authors.lastname: Bücking
authors.forename: Marcus
authors.initials: M
authors.lastname: Schicklberger
authors.forename: Johannes
authors.initials: J
authors.lastname: Gescher
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D045304:Cytochromes c / Q000032:analysis*; D004579:Electron Transport; D006090:Gram-Negative Bacteria / Q000201:enzymology*; D010084:Oxidation-Reduction; D012256:Riboflavin / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D045304:Cytochromes c; D012256:Riboflavin