Intrahepatic growth and maturation of Gnathostoma turgidum in the natural definitive opossum host, Didelphis virginiana.
Gnathostoma turgidum is a gastric nematode parasite of opossums found in the Americas. We recently found that G. turgidum juveniles appear in the liver of the opossums where they become mature adults and almost synchronously move to the stomach during certain months of the year, suggesting the importance of the liver for the growth and maturation of this species in the final hosts. In this study we attempted to detect G. turgidum larvae in the liver of opossums, Didelphis virginiana that are the natural final hosts. The results show that tiny (<3mm in length) third stage larvae (L3) appeared in the liver of opossums around November and December. Also in the liver, we found large L3 of up to about 10mm in length together with juveniles and mature adults from February to March. In spite of their length, large L3 have 4 rows of hooklets, and their gonads remained undeveloped. Morphological features of the small and large L3 of G. turgidum are described including scanning electron microscope images. The seasonal switching of the several growth stages of G. turgidum from small L3 to adult worms in the liver and eventual migration to the stomach in opossums suggests the unique feature of G. turgidum utilizing the liver as the maturation site.
Díaz-Camacho
Sylvia Páz
SP
Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México.
Delgado-Vargas
Francisco
F
Willms
Kaethe
K
de la Cruz-Otero
María del Carmen
Mdel C
Guadalupe Rendón-Maldonado
José
J
Robert
Lilia
L
Antuna
Silvia
S
Nawa
Yukifumi
Y
D000818:Animals; D018509:DNA, Helminth / Q000032:analysis / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D048293:Didelphis / Q000469:parasitology*; D006039:Gnathostoma / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D006790:Host-Parasite Interactions; D007814:Larva / Q000254:growth & development / Q000648:ultrastructure; D008099:Liver / Q000469:parasitology*; D008855:Microscopy, Electron, Scanning; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D017205:Spirurida Infections / Q000469:parasitology / Q000662:veterinary*; D013270:Stomach / Q000469:parasitology
D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer