title: Complete genomic sequence analysis of the temperate bacteriophage phiSASD1 of Streptomyces avermitilis.
abstract: The bacteriophage phiSASD1, isolated from a failed industrial avermectin fermentation, belongs to the Siphoviridae family. Its four predominant structural proteins, which include the major capsid, portal and two tail-related proteins, were separated and identified by SDS-PAGE and N-terminal sequence analysis. The entire double-stranded DNA genome of phiSASD1 consists of 37,068 bp, with 3'-protruding cohesive ends of nine nucleotides. Putative biological functions have been assigned to 24 of the 43 potential open reading frames. Comparative analysis shows perfect assembly of three "core" gene modules: the morphogenesis and head module, the tail module and the right arm gene module, which displays obvious similarity to the right arm genes of Streptomyces phage phiC31 in function and arrangement. Meanwhile, structural module flexibility within phiSASD1 suggests that assignment of phage taxonomy based on comparative genomics of structural genes will be more complex than expected due to the exchangeability of functional genetic elements.
authors.affiliation: Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing, PR China. wswwsw2001@hotmail.com
authors.forename: Shiwei
authors.initials: S
authors.lastname: Wang
authors.forename: Xuewei
authors.initials: X
authors.lastname: Qiao
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Xuejin
authors.initials: X
authors.lastname: Zhao
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Wen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Song
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017421:Sequence Analysis; D020539:Sequence Analysis, Protein; D017906:Siphoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D013302:Streptomyces / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D004247:DNA