title: L-methionine-induced alterations in molecular signatures in MCF-7 and LNCaP cancer cells.
abstract: BACKGROUND
  Methionine inhibits proliferation of breast and prostate cancer cells. Here, we determined the influence of L-methionine on functional molecular signatures in these cell lines.
  
  
  METHODS
  MCF-7 and LNCaP cells were treated with L-methionine (5 mg/ml) for 72 h. Changes in molecular signatures of these cells were examined by microarray analysis of 15,814 probes in triplicate experiments.
  
  
  RESULTS
  In LNCaP cells, 325 genes were up-regulated by methionine, and 517 genes down-regulated. In MCF-7 cells, 86 genes were up-regulated and 135 genes down-regulated. Ninety-eight genes were regulated in the same direction by methionine in both cells lines, and five other genes were changed in expression in opposite directions.
  
  
  CONCLUSIONS
  Several of the up-regulated genes encode proteins involved in cellular redox regulation, suggesting that methionine may enhance antioxidant mechanisms. Many of the down-regulated genes belong to protein kinase families that may be related to the anti-proliferative effects of methionine on breast and prostate cancer cells.
authors.affiliation: Department of Pathology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA, maxbenav@uic.edu
authors.forename: Maximo A
authors.initials: MA
authors.lastname: Benavides
authors.forename: Dong
authors.initials: D
authors.lastname: Hu
authors.forename: Marie Kristine
authors.initials: MK
authors.lastname: Baraoidan
authors.forename: Annette
authors.initials: A
authors.lastname: Bruno
authors.forename: Pan
authors.initials: P
authors.lastname: Du
authors.forename: Simon
authors.initials: S
authors.lastname: Lin
authors.forename: Wancai
authors.initials: W
authors.lastname: Yang
authors.forename: Kirby I
authors.initials: KI
authors.lastname: Bland
authors.forename: William E
authors.initials: WE
authors.lastname: Grizzle
authors.forename: Maarten C
authors.initials: MC
authors.lastname: Bosland
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D008297:Male; D008715:Methionine / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D008715:Methionine
references.citation: Int J Cancer. 1993 Sep 30;55(3):453-8
references.pmid: 8375929
references.citation: Cancer Res. 1989 Sep 1;49(17):4859-65
references.pmid: 2503245
references.citation: Antioxid Redox Signal. 2008 Sep;10(9):1501-14
references.pmid: 18498225
references.citation: Cell Mol Life Sci. 2001 Feb;58(2):244-58
references.pmid: 11289306
references.citation: Biol Direct. 2007 May 31;2:16
references.pmid: 17540033
references.citation: Nat Genet. 2008 Jul;40(7):844-53
references.pmid: 18511948
references.citation: Chem Biol Interact. 1998 Apr 24;111-112:1-14
references.pmid: 9679538
references.citation: Nat Genet. 2005 Jan;37(1):48-55
references.pmid: 15608639
references.citation: Nat Genet. 2003 Jun;34(2):226-30
references.pmid: 12754511
references.citation: Gene. 2002 May 29;291(1-2):233-9
references.pmid: 12095696
references.citation: J Cell Mol Med. 2004 Apr-Jun;8(2):201-12
references.pmid: 15256068
references.citation: Prostate. 2003 Mar 1;54(4):275-89
references.pmid: 12539226
references.citation: Stat Appl Genet Mol Biol. 2004;3:Article3
references.pmid: 16646809
references.citation: Curr Protoc Protein Sci. 2001 May;Appendix 4:Appendix 4K
references.pmid: 18429085
references.citation: Trends Biochem Sci. 2009 Feb;34(2):85-96
references.pmid: 19135374
references.citation: Nucleic Acids Res. 2008 Feb;36(2):e11
references.pmid: 18178591
references.citation: Mol Pharmacol. 2006 May;69(5):1662-72
references.pmid: 16478829
references.citation: Cancer Res. 2004 Oct 15;64(20):7610-7
references.pmid: 15492289
references.citation: J Nutr Biochem. 2008 Apr;19(4):207-15
references.pmid: 17707628
references.citation: Neurobiol Aging. 2004 Jan;25(1):63-9
references.pmid: 14675732
references.citation: Anticancer Res. 2010 Jun;30(6):1881-5
references.pmid: 20651330
references.citation: J Biol Chem. 2001 Nov 30;276(48):44379-84
references.pmid: 11567026
references.citation: Cancer Res. 1983 Apr;43(4):1809-18
references.pmid: 6831420
references.citation: Am J Surg. 2007 Feb;193(2):274-83
references.pmid: 17236862
references.citation: J Bioenerg Biomembr. 2002 Jun;34(3):209-19
references.pmid: 12171070
references.citation: Curr Pharm Des. 2009;15(6):675-81
references.pmid: 19199988
references.citation: Cancer Res. 1993 Nov 1;53(21):5193-8
references.pmid: 7693333
references.citation: Curr Pharm Des. 2009;15(8):893-916
references.pmid: 19275653
references.citation: Proteins. 2005 Oct 1;61(1):213-6
references.pmid: 16080146