title: The behaviour of both Listeria monocytogenes and rat ciliated ependymal cells is altered during their co-culture.
abstract: BACKGROUND
  Ciliated ependymal cells line the cerebral ventricles and aqueducts separating the infected CSF from the brain parenchyma in meningitis.
  
  
  RESULTS
  Investigation of the interaction of Listeria monocytogenes with cultured rat brain ependymal cells showed that certain strains reduced the beat frequency of the cilia but all the strains studied significantly reduced the ciliary beat amplitude (the linear distance travelled by the tip of each cilium per beat cycle).
  
  
  CONCLUSIONS
  The presence of the ependyma caused aggregation of some listeria strains and in some cases extracellular material also was seen in association with bacterial aggregates. These observations were dependent on the expression of genes required for invasion, intracellular survival and listerial cell to cell spread that are regulated by the transcriptional activator, positive regulatory factor A (PrfA).
authors.affiliation: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom.
authors.forename: Mina J
authors.initials: MJ
authors.lastname: Fadaee-Shohada
authors.forename: Robert A
authors.initials: RA
authors.lastname: Hirst
authors.forename: Andrew
authors.initials: A
authors.lastname: Rutman
authors.forename: Ian S
authors.initials: IS
authors.lastname: Roberts
authors.forename: Chris
authors.initials: C
authors.lastname: O'Callaghan
authors.forename: Peter W
authors.initials: PW
authors.lastname: Andrew
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D002417:Cattle; D002450:Cell Communication; D002923:Cilia / Q000378:metabolism* / Q000382:microbiology / Q000648:ultrastructure; D018920:Coculture Techniques / Q000379:methods*; D004805:Ependyma / Q000166:cytology* / Q000382:microbiology / Q000648:ultrastructure; D008089:Listeria monocytogenes / Q000166:cytology*; D010454:Peptide Termination Factors / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001426:Bacterial Proteins; D010454:Peptide Termination Factors; C545356:PrfA protein, Listeria monocytogenes
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