Identification of the functional domain of duck interleukin 2 binding to duck interleukin 2 receptor alpha chain.
Interleukin 2 (IL-2) plays an important role in the growth and differentiation of lymphocytes. To identify the functional domains of duck IL-2 (duIL-2), 4 neutralizing monoclonal antibodies (mAbs) to duIL-2 were used to finely map the functional domains of the duIL-2 protein. The mimotopes of 4 anti-duIL-2 mAbs, including LVXGSMPS, KPHKHHXHHSHM, WXXXKAKP, and HVPNERYPLR, were identified by phage display and peptide-competitive ELISA. These mimotopes constitute an important functional domain, Y(32) approximately T(44) (domain I), in the duIL-2 molecule. The bioactivity of the domain I peptide on in vitro lymphocyte proliferation was inhibited by duIL-2Ralpha. A tertiary structure model of duIL-2 showed that domain I is positioned in the long A-B loop and the N-terminal of Helix B. These data provided experimental evidence for elucidating the interaction between duIL-2 and duIL-2Ralpha.
Gu
Jianyou
J
Key Laboratory of Animal Epidemic Etiology & Immunological Prevention of Ministry of Agriculture, Zhejiang University, Hangzhou 310029, PR China.
Luo
Min
M
Ma
Guangpeng
G
Zhou
Jiyong
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D003198:Computer Simulation; D004372:Ducks / Q000276:immunology*; D018604:Epitope Mapping; D007376:Interleukin-2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D019151:Peptide Library; D011485:Protein Binding; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000276:immunology / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D019151:Peptide Library; D015375:Receptors, Interleukin-2