Complete genomic sequence analysis of the temperate bacteriophage phiSASD1 of Streptomyces avermitilis.
The bacteriophage phiSASD1, isolated from a failed industrial avermectin fermentation, belongs to the Siphoviridae family. Its four predominant structural proteins, which include the major capsid, portal and two tail-related proteins, were separated and identified by SDS-PAGE and N-terminal sequence analysis. The entire double-stranded DNA genome of phiSASD1 consists of 37,068 bp, with 3'-protruding cohesive ends of nine nucleotides. Putative biological functions have been assigned to 24 of the 43 potential open reading frames. Comparative analysis shows perfect assembly of three "core" gene modules: the morphogenesis and head module, the tail module and the right arm gene module, which displays obvious similarity to the right arm genes of Streptomyces phage phiC31 in function and arrangement. Meanwhile, structural module flexibility within phiSASD1 suggests that assignment of phage taxonomy based on comparative genomics of structural genes will be more complex than expected due to the exchangeability of functional genetic elements.
Wang
Shiwei
S
Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing, PR China. wswwsw2001@hotmail.com
Qiao
Xuewei
X
Liu
Xiaoxi
X
Zhang
Xiaolin
X
Wang
Chao
C
Zhao
Xuejin
X
Chen
Zhi
Z
Wen
Ying
Y
Song
Yuan
Y
D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017421:Sequence Analysis; D020539:Sequence Analysis, Protein; D017906:Siphoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D013302:Streptomyces / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000302:isolation & purification
D004279:DNA, Viral; D014764:Viral Proteins; D004247:DNA