In situ detection of pterins by SERS.
Surface enhanced Raman scattering (SERS) has been used to detect specific pterin molecules at sub-nanomolar concentrations. SERS is fast becoming a widely used technique for the sensitive and specific detection of multiple analytes. The information-rich and concentration-dependent spectra obtained from SERS make the technique ideally placed for high speed, low cost analysis of almost any analyte. Further, to show the feasibility of SERS in the detection of biologically relevant targets, a synthetic pterin analogue of the naturally occurring pterin cofactor, tetrahydrobiopterin, has been detected at a series of concentrations and the method used for the successful detection of the synthetic pterin in mouse serum. In this analysis, spectroscopic collection was optimized for water-based pteridine derivatives using two visible wavelengths of excitation (514.5 and 632.8 nm) and differing mesoscopic metal nanoparticles allowing the limits of detection to be calculated.
Stevenson
Ross
R
Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK G1 1XL.
Stokes
Robert J
RJ
MacMillan
Donna
D
Armstrong
David
D
Faulds
Karen
K
Wadsworth
Roger
R
Kunuthur
Suma
S
Suckling
Colin J
CJ
Graham
Duncan
D
D000818:Animals; D005240:Feasibility Studies; D057230:Limit of Detection; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D011622:Pterins / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013059:Spectrum Analysis, Raman / Q000379:methods*
D011622:Pterins