title: Response of human fibroblasts to implant surface coated with titanium dioxide photocatalytic films.
abstract: OBJECTIVE
  This study was to develop a titanium dioxide (TiO(2))-coated implant abutment, surface with ultraviolet (UV) light-induced hydrophilicity and investigate the initial response of human, fibroblasts to the surface modification.
  
  
  METHODS
  Commercially pure titanium (JIS 2 grade) disks were coated with TiO(2) to various, thicknesses (1, 2 or 3 μm) using peroxotitanium acid solution. The surface characteristics of each disk, were examined with X-ray diffraction (XRD), surface roughness equipment and scanning electron, microscopy (SEM). The hydrophilic change of each disk was determined by the contact angles at 0-24h, after 24-h UV irradiation. The biological response at the surface of each disk was examined by using, human periodontal ligament fibroblasts (HPLFs). The data were statistically analyzed with analysis of variance (ANOVA) and multiple-comparison tests.
  
  
  RESULTS
  The TiO(2)-coated disk surface had an anatase structure. Surface roughness did not differ, significantly among the disks; the surface morphology was smooth and had a hydrophilic or superhydrophilic, status. HPLF proliferation significantly increased on the TiO(2)-coated disks compared with the uncoated disks and depended upon the coated film thickness.
  
  
  CONCLUSIONS
  An anatase TiO(2)-coated surface under UV irradiation markedly improves the initial response of human fibroblasts.
authors.affiliation: Division of Fixed Prosthodontics, Department of Oral & Maxillofacial Rehabilitation, Kanagawa Dental College, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan. star@kdcnet.ac.jp
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Hoshi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Negishi
authors.forename: Shusaku
authors.initials: S
authors.lastname: Okada
authors.forename: Toru
authors.initials: T
authors.lastname: Nonami
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kimoto
mesh_terms: D001672:Biocompatible Materials; D002478:Cells, Cultured; D000044:Dental Abutments; D015921:Dental Implants; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D013499:Surface Properties; D014025:Titanium; D014466:Ultraviolet Rays
chemical_list: D001672:Biocompatible Materials; D015921:Dental Implants; C009495:titanium dioxide; D014025:Titanium