Time course of serum S100B protein and neuron-specific enolase levels of a single dose of chlorpyrifos in rats.
Organophosphate (OP) compounds are a large class of chemicals, many of which are used as pesticides. It is suggested that OPs specifically affect glia and neurons. Effects of acute exposure to chlorpyrifos (CPF), which is a common organophosphorus pesticide used worldwide, on neuron-specific enolase (NSE) and S100B levels in rat blood during 7 days were assessed. Rats were evaluated either before (0 hr) or 2, 12, 24, 48 and 168 hr (7 days) after injection of CPF (279 mg/kg, s.c.) or vehicle (peanut oil, 2 ml/kg, s.c.) for clinical signs of toxicity. Immediately after the evaluation of toxicity, blood samples were taken for biochemical assays. CPF administration produced decreases in body-weight and temperature, which were observed for first time at 12 hr after CPF administration and continued for 168 hr (p < 0.05-0.001). Serum S100B and NSE levels were acutely increased 2 hr after CPF administration and remained high at 12 hr (p < 0.01-0.001). NSE and S100B levels were not different in either CPF or vehicle groups at following time points. Serum butyrylcholinesterase (EC 3.1.1.8; BuChE) activity was dramatically reduced at 2 hr after CPF and remained low at each time points during 7 days (p < 0.01-0.001). Our results suggest that the usefulness of serum levels of these glia- and neuron-specific marker proteins in assessing OP toxicity, specifically CPF-induced toxicity.
Bozkurt
Ayhan
A
Department of Physiology, Ondokuz Mayıs University, School of Medicine, Samsun, Turkey. abozkurt@omu.edu.tr
Yardan
Turker
T
Ciftcioglu
Engin
E
Baydin
Ahmet
A
Hakligor
Aylin
A
Bitigic
Medine
M
Bilge
Sirri
S
D000818:Animals; D002091:Butyrylcholinesterase / Q000378:metabolism; D004390:Chlorpyrifos / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D007150:Immunohistochemistry; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D009414:Nerve Growth Factors / Q000097:blood*; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D020258:Neurotoxicity Syndromes / Q000097:blood / Q000201:enzymology / Q000209:etiology* / Q000503:physiopathology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000097:blood*; D013997:Time Factors
D007306:Insecticides; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579158:S100b protein, rat; D002091:Butyrylcholinesterase; D010751:Phosphopyruvate Hydratase; D004390:Chlorpyrifos