Additional layers of gene regulatory complexity from recently discovered microRNA mechanisms.
In recent years microRNAs have become recognized as pervasive, versatile agents of gene regulation. Some widely embraced rules involving Watson-Crick hybridization of microRNAs with mRNAs have generated great interest as scientists envision potential RNA cargoes for gene therapy and other experimental systems. However, while researchers ardently seek simplifying principles, nature seems very uncooperative. This article reviews some small RNA mechanisms that potentially regulate genes and which are not covered by previous microRNAs characterizations. In addition, we report here results of fluorescence microscopy experiments to directly demonstrate nuclear import of small RNAs equal in length to typical mature microRNAs, implying that gene regulation at the locus of transcription might be possible.
Jeffries
Clark D
CD
Renaissance Computing Institute and Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, CB 360, Chapel Hill, NC 27599, USA. clark_jeffries@med.unc.edu
Fried
Howard M
HM
Perkins
Diana O
DO
D021581:Active Transport, Cell Nucleus; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009831:Olfactory Mucosa / Q000166:cytology / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism
C527114:MIRN373 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering
J Theor Biol. 2006 Jul 21;241(2):205-15
16403535
Nat Struct Mol Biol. 2006 Sep;13(9):763-71
16936727
Genome Biol. 2008;9(8):R127
18700987
Cell Cycle. 2008 Jun 1;7(11):1545-9
18469529
Science. 2008 Jul 25;321(5888):537-41
18653886
Cell. 2008 Aug 22;134(4):577-86
18691745
Curr Top Microbiol Immunol. 2008;320:211-24
18268846
Nature. 2008 May 22;453(7194):534-8
18404147
Science. 2007 Dec 21;318(5858):1931-4
18048652
Nat Methods. 2008 Feb;5(2):117
18235428
Dev Biol. 2005 Oct 15;286(2):464-71
16154556
Nature. 2008 May 22;453(7194):539-43
18404146
Nature. 2008 Aug 7;454(7205):780-3
18596690
Cell Mol Life Sci. 2006 Jan;63(2):246-54
16395542
Nat Rev Mol Cell Biol. 2008 Sep;9(9):673-8
18719707
RNA. 2006 Feb;12(2):256-62
16373483
Nat Immunol. 2008 Sep;9(9):1065-73
18677316
Science. 2005 Sep 2;309(5740):1564-6
16141073
Nat Struct Mol Biol. 2008 Aug;15(8):842-8
18604220
Nucleic Acids Res. 2008 Nov;36(20):6439-49
18842624
J Biol Chem. 2007 Jan 26;282(4):2130-4
17135268
Nature. 2007 Jun 14;447(7146):799-816
17571346
Nature. 2008 Oct 23;455(7216):1124-8
18806776
Nature. 2005 Dec 1;438(7068):685-9
16258535
Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1608-13
18227514
Biochem Biophys Res Commun. 2006 Aug 18;347(1):36-43
16815300
BMC Genomics. 2006 Jun 01;7:133
16740159
Science. 2007 Jan 5;315(5808):97-100
17204650
Mol Cell. 2007 Jun 8;26(5):745-52
17540599
Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13421-6
18755897
Curr Biol. 2005 Jan 26;15(2):R61-4
15668159
Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17337-42
17085592
Nature. 2008 Sep 4;455(7209):58-63
18668040
Trends Biochem Sci. 2008 Oct;33(10):474-81
18774719
Biol Cell. 2008 Apr;100(4):201-17
18341479
Nature. 2004 Nov 11;432(7014):235-40
15531877
Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13556-61
18768788
Nat Chem Biol. 2007 Mar;3(3):166-73
17259978
Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):13906-11
18784367
Nat Struct Mol Biol. 2006 Sep;13(9):787-92
16936728
Trends Genet. 2006 Oct;22(10):532-6
16914224
Nature. 2008 Sep 4;455(7209):64-71
18668037