[Evaluation on a fast weight reduction model in vitro].
OBJECTIVE
To establish a fast and effective model in vitro for screening weight-reducing drugs and taking preliminary evaluation of the model.


METHODS
Mature adipocytes of SD rat induced by oleic acid were used to establish a obesity model in vitro. Isoprel, genistein, caffeine were selected as positive agents and curcumine as negative agent to evaluate the obesity model.


RESULTS
Lipolysis of adipocytes was stimulated significantly by isoprel, genistein and caffeine rather than curcumine.


CONCLUSIONS
This model could be used efficiently for screening weight-losing drugs.
Li
Songtao
S
Department of Nutrition and Food Hygene, College of Public Health, Harbin Medical University, Harbin 150086, China. lisongtaoklds@126.com
Li
Ying
Y
Wen
Ying
Y
Sun
Changhao
C
D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology; D002478:Cells, Cultured; D003474:Curcumin / Q000494:pharmacology; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical / Q000379:methods; D019833:Genistein / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008066:Lipolysis; D008297:Male; D019301:Oleic Acid / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D019440:Anti-Obesity Agents; D019301:Oleic Acid; D002110:Caffeine; D019833:Genistein; D003474:Curcumin; D007545:Isoproterenol