Fluorescent reporters for studies of cellular localization of proteins in Staphylococcus aureus.
We have constructed a set of plasmids that allow expression, from their native chromosomal loci, of Staphylococcus aureus proteins fused to one of four different fluorescent proteins (green fluorescent protein [GFP], cyan fluorescent protein [CFP], yellow fluorescent protein [YFP], and mCherry), using two different resistance markers (kanamycin and erythromycin). We have also constructed a plasmid that allows expression of proteins from the ectopic spa locus in the S. aureus chromosome. This toolbox can be used for studies of the localization of proteins in S. aureus, a prominent pathogen in both health care and community settings.
Pereira
Pedro M
PM
Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República, Apartado 127, 2781-901 Oeiras, Portugal.
Veiga
Helena
H
Jorge
Ana M
AM
Pinho
Mariana G
MG
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D013211:Staphylococcus aureus / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D001426:Bacterial Proteins; D008164:Luminescent Proteins
Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9642-7
10449747
Microbiol Mol Biol Rev. 1999 Mar;63(1):174-229
10066836
Appl Environ Microbiol. 2004 Nov;70(11):6887-91
15528558
Methods Enzymol. 1990;185:223-8
2116574
Nat Rev Microbiol. 2005 Dec;3(12):948-58
16322743
Infect Immun. 2001 Dec;69(12):7851-7
11705967
J Microbiol Methods. 2009 Jun;77(3):251-60
19264102
Appl Environ Microbiol. 2009 May;75(10):3034-8
19304835
Cell. 2008 Oct 3;135(1):74-84
18854156
J Bacteriol. 2005 Apr;187(7):2426-38
15774886
FEMS Microbiol Rev. 2008 Mar;32(2):345-60
18266741
Emerg Infect Dis. 2001 Mar-Apr;7(2):178-82
11294701
Eur J Biochem. 1981 Oct;119(2):389-93
7308191
Gene. 1995 Dec 29;167(1-2):335-6
8566804
J Bacteriol. 1977 Jan;129(1):343-50
830642
Appl Environ Microbiol. 2002 May;68(5):2624-8
11976148
Mol Microbiol. 2003 Nov;50(3):871-81
14617148
Mol Microbiol. 2004 May;52(3):801-14
15101985
Infect Immun. 2002 Mar;70(3):1326-33
11854217
Annu Rev Biochem. 1998;67:509-44
9759496
J Bacteriol. 1992 Aug;174(15):4952-9
1321119
Infect Immun. 1998 Dec;66(12):5988-93
9826382