Knockdown of transmembrane protein 132A by RNA interference facilitates serum starvation-induced cell death in Neuro2a cells.
Transmembrane protein 132A (TMEM132A) is a novel GRP78 binding protein that we recently discovered. However, the biological functions of TMEM132A are merely characterized because it does not encode any known structural domains. In this study, we down regulated intrinsic TMEM132A by RNA interference and identified a variety of genes that fluctuated during TMEM132A gene silencing using microarray analysis. TMEM132A-knockdown in Neuro2a cells caused neurite-like projection without any stimuli and enhanced the expression of ATF6 mRNA, an ER stress transducer, and GADD153 mRNA, a stress inducible gene. Under serum-deprived condition, TMEM132A-knockdown cells gradually retarded neurite-like projection and decreased cell viability. Moreover, TMEM132A knockdown markedly induced GADD153 expression due to serum starvation without affecting the level of cleaved caspase-3. Our data suggest that TMEM132A is an important factor of cell survival in regulating certain ER stress-related gene expression in neuronal cells.
Oh-hashi
Kentaro
K
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido, Gifu, Japan. oohashi@gifu-u.ac.jp
Imai
Kazuhide
K
Koga
Hisashi
H
Hirata
Yoko
Y
Kiuchi
Kazutoshi
K
D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D000091342:Endoplasmic Reticulum Chaperone BiP; D020869:Gene Expression Profiling; D020868:Gene Silencing / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013217:Starvation; D051742:Transcription Factor CHOP / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D016895:Culture Media, Serum-Free; C497250:Ddit3 protein, mouse; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D008565:Membrane Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C554679:TMEM132A protein, mouse; D051742:Transcription Factor CHOP; D053148:Caspase 3
Development. 2006 Nov;133(21):4151-62
17021036
FEBS Lett. 2006 Jul 10;580(16):3943-7
16806201
Genomics. 1999 Apr 1;57(1):120-9
10191091
Mol Cell Biol. 2007 Dec;27(23):8178-89
17893326
Genes Dev. 1998 Apr 1;12(7):982-95
9531536
Mol Cell Biol. 1997 Sep;17(9):4957-66
9271374
Cell Death Differ. 2007 Mar;14(3):625-33
16932756
Biochem J. 2002 Sep 1;366(Pt 2):585-94
12014989
Oncogene. 2001 Apr 5;20(15):1803-15
11313928
Nature. 2006 May 25;441(7092):513-7
16724068
Nature. 2001 Jul 5;412(6842):95-9
11452314
Nature. 2000 Jan 6;403(6765):98-103
10638761
Nature. 1999 Jan 21;397(6716):271-4
9930704
Cell Death Differ. 2004 Apr;11(4):381-9
14685163
Dev Cell. 2003 Apr;4(4):442-4
12689582
J Biol Chem. 2002 Feb 15;277(7):5460-7
11733516
Apoptosis. 2009 Nov;14(11):1274-88
19757066
Mol Biol Cell. 2008 Apr;19(4):1594-604
18256285
J Neurochem. 2005 Nov;95(4):974-86
16135078
Cell. 1997 Aug 8;90(3):405-13
9267021
Biochim Biophys Acta. 2002 Jun 13;1583(1):108-16
12069855
Biochim Biophys Acta. 2004 Oct 11;1685(1-3):48-62
15465426
Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):15920-5
12446838
J Biol Chem. 2003 Mar 21;278(12):10531-7
12514190
J Neurochem. 2006 Nov;99(3):952-64
16942595
Genes Dev. 1992 Mar;6(3):439-53
1547942
Neurobiol Aging. 2004 Jan;25(1):83-91
14675734
Biochem Pharmacol. 2008 Dec 1;76(11):1490-502
18761000
Am J Pathol. 2008 Feb;172(2):454-69
18187572
J Biol Chem. 2004 Dec 10;279(50):52007-15
15466475
Cell Death Differ. 2006 Mar;13(3):385-92
16397584
Biochem Biophys Res Commun. 2007 Apr 6;355(2):543-8
17307147
Nature. 2002 Jan 3;415(6867):92-6
11780124
Redox Rep. 2004;9(3):173-8
15327748