title: On-line vapor-phase generation followed by Fourier-transform infrared spectrometry for the quantitative analysis of water-soluble penicillin G in pharmaceutical formulations.
abstract: The combination of vapor-phase generation (VPG) and Fourier-transform infrared (FTIR) spectrometry was performed as an alternative analytical technique for the determination of water-soluble penicillin G (PENG). Samples were transferred into a heated reactor, and a potassium iodate solution was injected into the reactor. Carbon monoxide (CO) generated under these conditions was carried via a N(2) gas carrier stream inside the IR gas cell, and the corresponding FTIR spectra were continuously recorded as a function of time. Analytical measurements were made using the maximum absorbance of the CO band at 2170 +/- 4 cm(-1). Various factors influencing the analytical signals were evaluated and selected. The figures of merit of the proposed method involve a linear calibration curve over the range of 3 to 320 mg L(-1), a limit of detection of 0.5 mg L(-1) and a precision of 2.6%. The method was successfully applied to PENG determination in pharmaceutical preparations.
authors.affiliation: Agricultural, Medical and Industrial Research School, Nuclear Science and Technology Research Institute, P. O. Box 31485/498, Karaj, Iran. msadeghi@nrcam.org
authors.forename: Mahdi
authors.initials: M
authors.lastname: Sadeghi
authors.forename: Mohsen
authors.initials: M
authors.lastname: Zeeb
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Kalaee
mesh_terms: D002248:Carbon Monoxide / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D007452:Iodates / Q000737:chemistry; D057230:Limit of Detection; D009584:Nitrogen / Q000737:chemistry; D010400:Penicillin G / Q000032:analysis* / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D007452:Iodates; D004364:Pharmaceutical Preparations; D017680:Potassium Compounds; D014867:Water; D002248:Carbon Monoxide; C039693:potassium iodate; D009584:Nitrogen; D010400:Penicillin G