title: Optical microangiography provides depth-resolved images of directional ocular blood perfusion in posterior eye segment.
abstract: In this paper, we demonstrate that the optical microangiography (OAMG) is capable of depth-resolved imaging of directional blood perfusion within both retinal and choroid in the posterior segment of human eye. The study uses an OMAG system operating at 840 nm with an imaging speed at 27,000 A-scans per second. Sequentially registered multiple OMAG projection maps of small areas ( approximately 1 mm x 1 mm) are combined to provide directional blood flow images for a larger field of view. It takes approximately 3.7 sec to image a small area (1 x 1 mm(2)), and approximately 2.5 min for a larger field (3 x 3 mm(2)). Finally, we show superior performance of OMAG in providing functional images of capillary level microcirculation at different depths of retina and choroid that correlate well with the standard retinal pathology.
authors.affiliation: Oregon Health & Science University, Department of Biomedical Engineering, 3303 South West Bond Avenue, Portland, Oregon 97239, USA. wangr@ohsu.edu
authors.forename: Ruikang K
authors.initials: RK
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: An
authors.forename: Spencer
authors.initials: S
authors.lastname: Saunders
authors.forename: David J
authors.initials: DJ
authors.lastname: Wilson
mesh_terms: D000792:Angiography / Q000379:methods*; D001783:Blood Flow Velocity / Q000502:physiology; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D055420:Perfusion Imaging / Q000379:methods*; D012161:Retinal Artery / Q000166:cytology* / Q000502:physiology*
references.citation: Phys Med Biol. 2007 Oct 7;52(19):5897-907
references.pmid: 17881807
references.citation: Opt Express. 2009 May 25;17(11):8926-40
references.pmid: 19466142
references.citation: Opt Express. 2008 Jul 21;16(15):11438-52
references.pmid: 18648464
references.citation: Am J Ophthalmol. 1997 Nov;124(5):677-82
references.pmid: 9372722
references.citation: BMJ. 1992 Sep 19;305(6855):678-83
references.pmid: 1393111
references.citation: Prog Retin Eye Res. 2002 Jul;21(4):359-93
references.pmid: 12150988
references.citation: Opt Express. 2007 Sep 3;15(18):11402-12
references.pmid: 19547498
references.citation: Opt Express. 2007 Apr 2;15(7):4083-97
references.pmid: 19532651