[Synthesis of immunomagnetic sorbents for separation of hepatitis B and C viruses].
This study was devoted to the creation of biomagnetic sorbents, capable of removing the virus particles from the human blood serum. With this purpose specific globulins were isolated from reconvalescents sera after acute hepatitis B virus (HBV) and hepatitis C virus (HCV), which were later used as molecules-vectors (ligands). Ligands were covalently bound to magnetite encapsulated by the matrix, carrying aminopropyl groups on its surface. The dynamics of immunoglobulin sorption with magnet controllable bearer was studied. Sera which were positive by DNA HBV, by RNA HCVin polymerase-chain reaction (PCR) and by surface antigen HBsAg in immunosorbent assay (ELISA) were approbated with the purpose of virus separation by immunomagnetic sorbents. Completed virus decontamination of the serum was achieved according to results of PCR on DNA HBV, at the same time the results of ELISA testify to the presence of HBsAg in the tested samples of the serum, it can be associated with the presence of many defect virus particles in population or with the lack of biomagnetic sorbent for complete elimination. Results ofquality PCR on RNA HCV of sera after decontamination testify to the absence of complete HCV elimination.
Vergun
L Iu
LIu
Klimchuk
D A
DA
Gorbik
P P
PP
Bondar'
L A
LA
Perekhristenko
P M
PM
D000327:Adsorption; D052203:Ferrosoferric Oxide / Q000737:chemistry; D016174:Hepacivirus / Q000302:isolation & purification*; D006509:Hepatitis B / Q000097:blood / Q000628:therapy / Q000821:virology*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006526:Hepatitis C / Q000097:blood / Q000628:therapy / Q000821:virology*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D018189:Immunomagnetic Separation / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D013499:Surface Properties
D007074:Immunoglobulin G; D011437:Propylamines; D012821:Silanes; C477625:amino-propyl-triethoxysilane; D052203:Ferrosoferric Oxide