LEAFPROCESSOR: a new leaf phenotyping tool using contour bending energy and shape cluster analysis.
*Significant progress has been made in the identification of the genetic factors controlling leaf shape. However, no integrated solution for the quantification and categorization of leaf form has been developed. In particular, the analysis of local changes in margin growth, which define many of the differences in shape, remains problematical. *Here, we report on a software package (LEAFPROCESSOR) which provides a semi-automatic and landmark-free method for the analysis of a range of leaf-shape parameters, combining both single metrics and principal component analysis. In particular, we explore the use of bending energy as a tool for the analysis of global and local leaf perimeter deformation. *As a test case for the implementation of the LEAFPROCESSOR program, we show that this integrated analysis leads to deeper insights into the morphogenic changes underpinning a series of previously identified Arabidopsis leaf-shape mutants. Our analysis reveals that many of these mutants which, at first sight, show similar leaf morphology, can be distinguished via our shape analysis. *The LEAFPROCESSOR program provides a novel integrated tool for the analysis of leaf shape.
Backhaus
Andreas
A
Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
Kuwabara
Asuka
A
Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
Bauch
Marion
M
Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
Monk
Nick
N
School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
Sanguinetti
Guido
G
Department of Computer Science, University of Sheffield, Regent Court, 211 Portobello, Sheffield S1 4DP, UK.|Department of Chemical and Process Engineering, University of Sheffield, Mappin Street, Sheffield S1 3JD, UK.
Fleming
Andrew
A
Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
D017360:Arabidopsis / Q000033:anatomy & histology* / Q000502:physiology*; D016000:Cluster Analysis; D016002:Discriminant Analysis; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D009154:Mutation / Q000235:genetics; D009929:Organ Size; D010641:Phenotype; D018515:Plant Leaves / Q000033:anatomy & histology* / Q000502:physiology*; D025341:Principal Component Analysis; D012984:Software; D013816:Thermodynamics
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