title: Role of BC loop residues in structure, function and antigenicity of the West Nile virus envelope protein receptor-binding domain III.
abstract: Site-directed mutagenesis of residues in the BC loop (residues 329-333) of the envelope (E) protein domain III in a West Nile virus (WNV) infectious clone and in plasmids encoding recombinant WNV and dengue type 2 virus domain III proteins demonstrated a critical role for residues in this loop in the function and antigenicity of the E protein. This included a strict requirement for the tyrosine at residue 329 of WNV for virus viability and E domain III folding. The absence of an equivalent residue in this region of yellow fever group viruses and most tick-borne flavivirus suggests there is an evolutionary divergence in the molecular mechanisms of domain III folding employed by different flaviviruses.
authors.affiliation: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Shuliu
authors.initials: S
authors.lastname: Zhang
authors.forename: Evgeniy I
authors.initials: EI
authors.lastname: Bovshik
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Maillard
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Gromowski
authors.forename: David E
authors.initials: DE
authors.lastname: Volk
authors.forename: Catherine H
authors.initials: CH
authors.lastname: Schein
authors.forename: Claire Y-H
authors.initials: CY
authors.lastname: Huang
authors.forename: David G
authors.initials: DG
authors.lastname: Gorenstein
authors.forename: James C
authors.initials: JC
authors.lastname: Lee
authors.forename: Alan D T
authors.initials: AD
authors.lastname: Barrett
authors.forename: David W C
authors.initials: DW
authors.lastname: Beasley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D057134:Antibodies, Neutralizing / Q000276:immunology / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D002522:Chlorocebus aethiops; D005260:Female; D006801:Humans; D051379:Mice; D050296:Microbial Viability; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009500:Neutralization Tests; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D053585:Virus Attachment; D014902:West Nile virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins
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