title: An uncoupling screen for autonomous embryo mutants in Arabidopsis thaliana.
abstract: Simple de novo screens in Arabidopsis thaliana have previously identified mutants that affect endosperm development but viable-embryo mutants have not been identified. Our strategy to identify autonomous embryo development was to uncouple embryo and endosperm fertilisation. This involved a male-sterile mutant population being crossed with a distinct pollen parent--the pollen was needed to initiate endosperm development and because it was distinct, the maternal progeny could be selected from the hybrid population. This process was refined over three stages, resulting in a viable approach to screen for autonomous embryo mutants. From 8,000 screened plants, a mutation was isolated in which the integument cells extended from the ovule and proliferated into a second complete twinned ovule. Some embryos from the mutant were normal but others developed fused cotyledons. In addition, a proportion of the progeny lacked paternal genes.
authors.affiliation: Department of Biology and Biochemistry, Bath University, Bath BA2 7AY, UK.
authors.forename: Nick
authors.initials: N
authors.lastname: Fenby
authors.forename: Hong
authors.initials: H
authors.lastname: Pu
authors.forename: Roger
authors.initials: R
authors.lastname: Pennell
authors.forename: Uta
authors.initials: U
authors.lastname: Praekelt
authors.forename: Rob
authors.initials: R
authors.lastname: Day
authors.forename: Rod
authors.initials: R
authors.lastname: Scott
mesh_terms: D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D056625:Endosperm / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D005306:Fertilization; D005821:Genetic Techniques; D009154:Mutation; D056771:Ovule / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011058:Pollen / Q000196:embryology / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins
references.citation: Development. 2006 Jan;133(2):251-61
references.pmid: 16339187
references.citation: Plant Physiol. 2001 Nov;127(3):803-16
references.pmid: 11706164
references.citation: Plant Cell. 1993 Jun;5(6):621-630
references.pmid: 12271078
references.citation: Curr Biol. 2005 Apr 26;15(8):750-4
references.pmid: 15854908
references.citation: Development. 1997 May;124(10):2049-62
references.pmid: 9169851
references.citation: Plant Cell. 1996 Feb;8(2):155-68
references.pmid: 8742707
references.citation: Plant Cell. 1991 Jul;3(7):677-684
references.pmid: 12324609
references.citation: Nature. 2008 Feb 28;451(7182):1121-4
references.pmid: 18272967
references.citation: Sex Plant Reprod. 2001 Dec;14(4):195-200
references.pmid: 24573426
references.citation: Plant Biotechnol J. 2003 Jan;1(1):33-42
references.pmid: 17147678
references.citation: Science. 1997 Jul 4;277(5322):91-4
references.pmid: 9204906
references.citation: Development. 2000 Jun;127(11):2493-502
references.pmid: 10804189
references.citation: Genetics. 1997 Apr;145(4):1109-24
references.pmid: 9093862
references.citation: Development. 2003 Jun;130(12):2555-65
references.pmid: 12736201
references.citation: Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13839-44
references.pmid: 10570159
references.citation: Plant Cell. 1994 Dec;6(12):1731-1745
references.pmid: 12244233
references.citation: Am J Bot. 2005 Nov;92(11):1797-810
references.pmid: 21646097
references.citation: Theor Appl Genet. 1995 Jun;90(7-8):1198-203
references.pmid: 24173084
references.citation: Theor Appl Genet. 1993 Jan;85(5):632-8
references.pmid: 24195939
references.citation: Plant Cell. 1994 Mar;6(3):385-392
references.pmid: 12244241
references.citation: Proc Natl Acad Sci U S A. 1996 May 28;93(11):5319-24
references.pmid: 11607683
references.citation: EMBO J. 1998 Mar 2;17(5):1405-11
references.pmid: 9482737
references.citation: Plant Cell. 1994 Aug;6(8):1049-1064
references.pmid: 12244265
references.citation: Science. 1998 Apr 17;280(5362):446-50
references.pmid: 9545225
references.citation: Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):4223-8
references.pmid: 9108133
references.citation: Development. 2004 Jan;131(2):389-400
references.pmid: 14681187
references.citation: Plant J. 2002 Mar;29(5):581-94
references.pmid: 11874571
references.citation: Plant Cell. 2003 Jun;15(6):1430-42
references.pmid: 12782734
references.citation: Plant Cell. 1996 Feb;8(2):137-53
references.pmid: 8742706
references.citation: Plant Cell. 1995 Mar;7(3):333-345
references.pmid: 12242374
references.citation: Nucleic Acids Res. 1991 Mar 25;19(6):1349
references.pmid: 2030957
references.citation: Plant Cell. 1992 Oct;4(10):1237-1249
references.pmid: 12297633
references.citation: Plant J. 2006 May;46(3):522-31
references.pmid: 16623911
references.citation: Development. 2004 Jun;131(12):2971-81
references.pmid: 15151989
references.citation: Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11806-11
references.pmid: 11573014
references.citation: Cell. 1998 Jun 26;93(7):1195-205
references.pmid: 9657152