Facile and rapid manipulation of DNA surface density on gold nanoparticles using mononucleotide-mediated conjugation.
We report here a method to manipulate the amount of thiolated DNA attached per gold nanoparticle from a few to tens of strands in less than an hour, which involves the tuning of the respective amount of salt and time of addition of thiolated oligo(ethylene glycol) during mononucleotide-mediated conjugation process.
Zhao
Wenting
W
Bioengineering Graduate Program, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
Hsing
I-Ming
IM
D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D009841:Oligonucleotides; D013438:Sulfhydryl Compounds; D006046:Gold; D004247:DNA