spa typing and enterotoxin gene profile of Staphylococcus aureus isolated from bovine raw milk in Korea.
Staphylococcus aureus is a major etiological pathogen of bovine mastitis, which triggers significant economic losses in dairy herds worldwide. In this study, S. aureus strains isolated from the milk of cows suffering from mastitis in Korea were investigated by spa typing and staphylococcal enterotoxin (SE) gene profiling. Forty-four S. aureus strains were isolated from 26 farms in five provinces. All isolates grouped into five clusters and two singletons based on 14 spa types. Cluster 1 and 2 isolates comprised 38.6% and 36.4% of total isolates, respectively, which were distributed in more than four provinces. SE and SE-like toxin genes were detected in 34 (77.3%) isolates and the most frequently detected SE gene profile was seg, sei, selm, seln, and selo genes (16 isolates, 36.3%), which was comparable to one of the genomic islands, Type I nuSabeta. This is a first report of spa types and the prevalence of the recently described SE and SE-like toxin genes among S. aureus isolates from bovine raw milk in Korea. Two predominant spa groups were distributed widely and recently described SE and SE-like toxin genes were detected frequently.
Hwang
Sun Young
SY
Department of Microbiology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
Park
Young Kyung
YK
Koo
Hye Cheong
HC
Park
Yong Ho
YH
D000818:Animals; D002417:Cattle; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004768:Enterotoxins / Q000737:chemistry / Q000235:genetics*; D005260:Female; D005838:Genotype; D008414:Mastitis, Bovine / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology*; D018895:Microsatellite Repeats; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D056910:Republic of Korea / Q000453:epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D004768:Enterotoxins
Vet Microbiol. 2009 May 28;137(1-2):202-6
19195799
Foodborne Pathog Dis. 2006 Fall;3(3):274-83
16972776
Mol Microbiol. 2001 Jul;41(2):365-77
11489124
Lancet. 2002 May 25;359(9320):1819-27
12044378
J Clin Microbiol. 1992 Jul;30(7):1654-60
1629319
Lancet. 2001 Apr 21;357(9264):1225-40
11418146
Infect Immun. 2006 Aug;74(8):4724-34
16861660
J Clin Microbiol. 1995 Feb;33(2):376-80
7714195
J Clin Microbiol. 1993 Mar;31(3):706-7
8458968
J Nat Toxins. 1998 Oct;7(3):193-213
9783259
Vet Microbiol. 1999 Sep 15;69(3):217-24
10512046
J Med Microbiol. 2008 Sep;57(Pt 9):1106-1112
18719180
Appl Environ Microbiol. 2007 Jun;73(12):3845-9
17449696
J Immunol. 2001 Jan 1;166(1):669-77
11123352
J Clin Microbiol. 2003 Dec;41(12):5442-8
14662923
Infect Immun. 2003 Oct;71(10):6088-94
14500536
J Appl Microbiol. 2006 Oct;101(4):864-71
16968298
Int J Food Microbiol. 2007 Jun 10;117(1):99-105
17439826
Eur J Clin Microbiol Infect Dis. 1996 Jan;15(1):60-4
8641305
J Clin Microbiol. 2000 Mar;38(3):1008-15
10698988
FEMS Microbiol Lett. 1998 Nov 15;168(2):227-33
9835033
Vet Microbiol. 2005 Feb 25;105(3-4):301-5
15708828
J Biol Chem. 2002 Apr 12;277(15):13138-47
11821418
Infect Immun. 1997 Oct;65(10):4048-54
9317006
J Dairy Sci. 2007 Apr;90(4):1716-24
17369211
J Clin Microbiol. 2006 Jul;44(7):2533-40
16825376
J Bacteriol. 2005 Apr;187(7):2426-38
15774886
J Appl Microbiol. 2000 Jun;88(6):1028-37
10849179
Vet Microbiol. 2001 Feb 26;78(4):373-82
11182503
Epidemiol Infect. 1997 Oct;119(2):261-9
9363026
J Korean Med Sci. 2009 Aug;24(4):585-91
19654937
J Clin Microbiol. 2004 Feb;42(2):792-9
14766855
J Appl Bacteriol. 1992 Jun;72(6):486-9
1644704
J Clin Microbiol. 1999 Nov;37(11):3556-63
10523551
Int J Food Microbiol. 2000 Oct 1;61(1):1-10
11028954
J Dairy Sci. 1992 Feb;75(2):596-605
1560148
J Dairy Sci. 2007 Dec;90(12):5405-14
18024731
FEMS Microbiol Lett. 2005 May 15;246(2):191-8
15899405
Clin Microbiol Rev. 2000 Jan;13(1):16-34, table of contents
10627489
Infect Immun. 2005 Sep;73(9):5540-6
16113270
J Clin Microbiol. 2008 Aug;46(8):2805-8
18524961
Clin Microbiol Rev. 2004 Jan;17(1):14-56
14726454
Appl Environ Microbiol. 1995 Apr;61(4):1438-43
7747964