Separation/determination of flavonoids and ascorbic acid in rat serum and excrement by capillary electrophoresis with electrochemical detection.
A capillary electrophoresis with electrochemical detection was developed for the simultaneous determination of three flavonoids (naringenin, rutin, quercetin) and ascorbic acid. It was found that naringenin, rutin, quercetin and ascorbic acid were well separated within 5 min in borate buffer solution (pH 8.6, 24 mM). The detection limit was 1.0 microM for naringenin, 8.0 microM for rutin, 2.0 microM for ascorbic acid and 0.5 microM for quercetin. The protocol was successfully applied for the determination of the analytes in rat serum and excrement. Recovery results ranged from 90.9 to 108.6%.
Qian
Xiaomin
X
Institute of Analytical Chemistry, Department of Chemistry, Dushu Lake Campus, Suzhou University, Suzhou 215123, P. R. China.
Zhang
Qianli
Q
Zhang
Ying
Y
Tu
Yifeng
Y
D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis / Q000097:blood* / Q000302:isolation & purification*; D002021:Buffers; D004563:Electrochemistry; D004566:Electrodes; D019075:Electrophoresis, Capillary; D005243:Feces / Q000737:chemistry*; D044950:Flavanones; D005419:Flavonoids / Q000032:analysis / Q000097:blood* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D011794:Quercetin; D051381:Rats; D015203:Reproducibility of Results; D012431:Rutin; D013997:Time Factors
D002021:Buffers; D044950:Flavanones; D005419:Flavonoids; D012431:Rutin; D011794:Quercetin; C005273:naringenin; D001205:Ascorbic Acid