title: Epidemiology of Escherichia coli clinical isolates producing AmpC plasmidic beta-lactamase during a 5-year period in a French teaching Hospital.
abstract: We investigated the prevalence and epidemiology of AmpC plasmidic cephalosporinases in Escherichia coli clinical strains resistant to third-generation cephalosporins during a 5-year period at Nantes University Hospital, France (3100 beds). The prevalence and diversity of plasmidic cephalosporinase did not increase during the study period (0.09% of 25 861 E. coli isolates); only CMY-2 producers were detected (and 1 new variant, with a Y-to-C substitution at position 219). CMY-2-producing strains belonged to the 4 main phylogenetic groups and to 11 different sequence types. Three sequence types included more than 1 isolate (ST156, ST46, and ST354).
authors.affiliation: CHU de Nantes, Service de Bactériologie-Hygiène, Nantes, France. stephane.corvec@chu-nantes.fr
authors.forename: Stéphane
authors.initials: S
authors.lastname: Corvec
authors.forename: Lise
authors.initials: L
authors.lastname: Crémet
authors.forename: Céline
authors.initials: C
authors.lastname: Leprince
authors.forename: Sandie
authors.initials: S
authors.lastname: Dauvergne
authors.forename: Alain
authors.initials: A
authors.lastname: Reynaud
authors.forename: Didier
authors.initials: D
authors.lastname: Lepelletier
authors.forename: Nathalie
authors.initials: N
authors.lastname: Caroff
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis*; D015373:Bacterial Typing Techniques; D002511:Cephalosporins / Q000494:pharmacology; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology; D005602:France / Q000453:epidemiology; D005838:Genotype; D006784:Hospitals, Teaching; D006801:Humans; D010957:Plasmids; D015995:Prevalence; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002511:Cephalosporins; D004269:DNA, Bacterial; C048795:AmpC beta-lactamases; D001618:beta-Lactamases