Molecular cloning and expression of a new gene, GON-SJTU1 in the rat testis.
BACKGROUND
Spermatogenesis is a complex process involving cell development, differentiation and apoptosis. This process is governed by a series of genes whose expressions are highly regulated. Male infertility can be attributed to multiple genetic defects or alterations that are related to spermatogenesis. The discovery, cloning and further functional study of genes related to spermatogenesis is of great importance to the elucidation of the molecular mechanism of spermatogenesis. It is also physiologically and pathologically significant to the therapy of male infertility.


METHODS
GON-SJTU1 was identified and cloned from rat testis by cDNA library screening and 3'-and 5'-RACE. The products of GON-SJTU1 were assessed by Northern and Western blotting. The expression of GON-SJTU1 was also examined by In situ hybridization and immunohistochemistry.


RESULTS
Here we identified and cloned a new gene, GON-SJTU1, with the biological process of spermatogenesis. GON-SJTU1 is highly expressed in the testis from day 1 to 15 and then decreased, suggesting that GON-SJTU1 might be a time-related gene and involved in the early stage of spermatogenesis. And the expression of GON-SJTU1 in the testis occurred in some male germ cells, particularly in gonocytes and spermatogonial stem cells.


CONCLUSIONS
GON-SJTU1 may play a role in the biological process of spermatogenesis.
Yang
Zhao-juan
ZJ
School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Sun
Ning
N
Wang
Shu-qin
SQ
Tian
Geng G
GG
Wu
Ji
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D008297:Male; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D051381:Rats; D029589:Seminal Plasma Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution
C552517:GON-SJTU1 protein, rat; D010750:Phosphoproteins; D029589:Seminal Plasma Proteins
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