title: Physico-chemical and biological properties of solvent/detergent treated immunoglobuin G preparations.
abstract: Protein composition homogeneity, fraction composition, molecular weight composition, anticomplementary activity, anti-A and anti-B haemagglutinins, specific activity, and pyrogens were studied in intramuscular and intravenous immunoglobulin G preparations, treated and non-treated with solvent/detergent mixture for virus inactivation. Physico-chemical and biological properties retention is shown. Quality of immunoglobulin preparations was improved due to purification by the methods of anion- and cation- exchange preparative low pressure liquid chromatography.
authors.affiliation: Zabolotny Institute of Microbiology and Virology National Academy of Sciences, 154 Zabolotny St., Kyiv, MSP, DO3680, Ukraine
authors.forename: N V
authors.initials: NV
authors.lastname: Nesterova
authors.forename: O V
authors.initials: OV
authors.lastname: Kurkina
authors.forename: V A
authors.initials: VA
authors.lastname: Samoilenko
authors.forename: M M
authors.initials: MM
authors.lastname: Skrynnyk
mesh_terms: D001681:Biological Assay; D055598:Chemical Phenomena; D002851:Chromatography, High Pressure Liquid; D003165:Complement System Proteins / Q000378:metabolism; D003902:Detergents / Q000737:chemistry*; D004339:Drug Compounding; D004340:Drug Contamination / Q000517:prevention & control; D004591:Electrophoresis, Polyacrylamide Gel; D006388:Hemagglutinins / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*; D016756:Immunoglobulins, Intravenous / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry*; D038301:Virus Inactivation; D014780:Viruses / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D003902:Detergents; D006388:Hemagglutinins; D006514:Hepatitis B Surface Antigens; D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous; D012997:Solvents; D003165:Complement System Proteins