Short-chain fructooligosaccharide regulates hepatic peroxisome proliferator-activated receptor alpha and farnesoid X receptor target gene expression in rats.
Prebiotic short-chain fructooligosaccharide (scFOS) is known to have various beneficial effects in humans and animals. Using a nutrigenomic approach, we have previously identified marker genes for the intestinal immunomodulatory and lipid-lowering effects of scFOS. The present study aimed to predict novel physiological effects of scFOS through nutrigenomic analyses. DNA microarray analysis revealed that administration of scFOS changed the expression of the nuclear receptors peroxisome proliferator-activated receptor alpha (PPARalpha) and farnesoid X receptor (FXR) target genes in the rat liver. Gene expression analysis provided some new interesting hypotheses, for instance, the possible improvement of bile secretion via FXR target genes, and regulation of amino acid metabolism and the urea cycle via PPARalpha and/or FXR target genes. Our findings clearly indicated that nutrigenomics may make it possible to screen for novel physiological effects of dietary ingredients.
Fukasawa
Tomoyuki
T
Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd, 5-3-1 Chiyoda, Sakado-shi, Saitama 350-0289, Japan. tomoyuki_fukasawa@meiji.co.jp
Kamei
Asuka
A
Watanabe
Yuki
Y
Koga
Jinichiro
J
Abe
Keiko
K
D000818:Animals; D015870:Gene Expression / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology*; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D000099150:Receptor, Farnesoid X-Activated
D009844:Oligosaccharides; D047493:PPAR alpha; D018160:Receptors, Cytoplasmic and Nuclear; D000099150:Receptor, Farnesoid X-Activated; C116580:fructooligosaccharide