Hemopoietic and angiogenetic progenitors in healthy athletes: different responses to endurance and maximal exercise.
The effects of endurance or maximal exercise on mobilization of bone marrow-derived hemopoietic and angiogenetic progenitors in healthy subjects are poorly defined. In 10 healthy amateur runners, we collected venous blood before, at the end of, and the day after a marathon race (n = 9), and before and at the end of a 1.5-km field test (n = 8), and measured hemopoietic and angiogenetic progenitors by flow cytometry and culture assays, as well as plasma or serum concentrations of several cytokines/growth factors. After the marathon, CD34(+) cells were unchanged, whereas clonogenetic assays showed decreased number of colonies for both erythropoietic (BFU-E) and granulocyte-monocyte (CFU-GM) series, returning to baseline the morning post-race. Conversely, CD34(+) cells, BFU-E, and CFU-GM increased after the field test. Angiogenetic progenitors, assessed as CD34(+)KDR(+) and CD133(+)VE-cadherin(+) cells or as adherent cells in culture expressing endothelial markers, increased after both endurance and maximal exercise but showed a different pattern between protocols. Interleukin-6 increased more after the marathon than after the field test, whereas hepatocyte growth factor and stem cell factor increased similarly in both protocols. Plasma levels of angiopoietin (Ang) 1 and 2 increased after both types of exercise, whereas the Ang-1-to-Ang-2 ratio or vascular endothelial growth factor-A were little affected. These data suggest that circulating hemopoietic progenitors may be utilized in peripheral tissues during prolonged endurance exercise. Endothelial progenitor mobilization after exercise in healthy trained subjects appears modulated by the type of exercise. Exercise-induced increase in growth factors suggests a physiological trophic effect of exercise on the bone marrow.
Bonsignore
Maria R
MR
Biomedical Department, Internal and Specialistic Medicine (DIBIMIS), Section of Pneumology, University of Palermo, Via Trabucco, 180, 90146 Palermo, Italy. marisa@ibim.cnr.it
Morici
Giuseppe
G
Riccioni
Roberta
R
Huertas
Alice
A
Petrucci
Eleonora
E
Veca
Mario
M
Mariani
Gualtiero
G
Bonanno
Anna
A
Chimenti
Laura
L
Gioia
Maria
M
Palange
Paolo
P
Testa
Ugo
U
D000071916:AC133 Antigen; D000328:Adult; D043925:Angiogenesis Inducing Agents / Q000097:blood; D015703:Antigens, CD / Q000097:blood; D018952:Antigens, CD34 / Q000097:blood; D056352:Athletes; D015820:Cadherins / Q000097:blood; D016207:Cytokines / Q000097:blood; D042783:Endothelial Cells / Q000502:physiology*; D015672:Erythroid Precursor Cells / Q000502:physiology*; D006023:Glycoproteins / Q000097:blood; D006098:Granulocytes / Q000502:physiology; D016298:Hematopoietic Cell Growth Factors / Q000097:blood; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D010455:Peptides / Q000097:blood; D010807:Physical Endurance / Q000502:physiology*; D012420:Running / Q000502:physiology
D000071916:AC133 Antigen; D043925:Angiogenesis Inducing Agents; D015703:Antigens, CD; D018952:Antigens, CD34; D015820:Cadherins; D016207:Cytokines; D006023:Glycoproteins; D016298:Hematopoietic Cell Growth Factors; C000606023:PROM1 protein, human; D010455:Peptides