title: Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials.
abstract: The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro. The in vivo study consisted in the analysis of the elimination and biodistribution kinetics of SPIONs after intravenous administration in Wistar rats. The results were corroborated by X-ray fluorescence. For the in vitro study, a quantitative analysis of the concentration of SPIONs bound to the specific AC133 monoclonal antibodies was carried out in order to detect the expression of the antigenic epitopes (CD133) in stem cells from human umbilical cord blood. In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).
authors.affiliation: Instituto Israelita de Ensino e Pesquisa Albert Einstein, IIEPAE, São Paulo, Brazil. lgamarra@einstein.br
authors.forename: Lionel F
authors.initials: LF
authors.lastname: Gamarra
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: daCosta-Filho
authors.forename: Javier B
authors.initials: JB
authors.lastname: Mamani
authors.forename: Rita
authors.initials: R
authors.lastname: de Cassia Ruiz
authors.forename: Lorena F
authors.initials: LF
authors.lastname: Pavon
authors.forename: Tatiana T
authors.initials: TT
authors.lastname: Sibov
authors.forename: Ernanni D
authors.initials: ED
authors.lastname: Vieira
authors.forename: André C
authors.initials: AC
authors.lastname: Silva
authors.forename: Walter M
authors.initials: WM
authors.lastname: Pontuschka
authors.forename: Edson
authors.initials: E
authors.lastname: Amaro
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000493:pharmacokinetics*; D003911:Dextrans / Q000493:pharmacokinetics*; D052203:Ferrosoferric Oxide / Q000493:pharmacokinetics*; D008280:Magnetics; D058185:Magnetite Nanoparticles; D008297:Male; D008657:Metabolic Clearance Rate; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; C097921:ferumoxtran-10; D052203:Ferrosoferric Oxide
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