ISOLATION OF CAFFEIC ACID FROM EUPATORIUM ADENOPHORUM SPRENG BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY AND SYNTHESIS OF CAFFEIC ACID-INTERCALATED LAYERED DOUBLE HYDROXIDE.
Preparative high-speed countercurrent chromatography (HSCCC) was successfully used for isolation and purification of caffeic acid from Eupatorium adenophorum Spreng with a solvent system composed of ethyl acetate-methanol-water at a volume ratio of 10:1:10, v/v. Using a preparative unit of the HSCCC centrifuge, about a 938 mg amount of the crude extract was separated, yielding 63.2 mg of caffeic acid at purity of 96.0%. Then, the anti-microbial and anti-virus drug caffeic acid (C(9)H(8)O(4)) was intercalated into layered double hydroxides for the first time by anion exchange under a nitrogen atmosphere. The product caffeic acid-LDH has been characterized by powder X-ray diffraction (XRD), Fourier transform-infrared (FT-IR), Scanning electron micrographs (SEM), indicating that the drug has been successfully intercalated into LDH.
Wei
Yun
Y
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, China.
Gao
Yali
Y
Zhang
Kai
K
Ito
Yoichiro
Y
J Control Release. 2002 Aug 21;82(2-3):417-27
12175754
Bioresour Technol. 2008 Jun;99(9):3729-36
17709243
Zhongguo Zhong Yao Za Zhi. 2001 Jan;26(1):45-7
12525120
J Chromatogr A. 2007 Nov 16;1172(1):40-6
17942106
J Chromatogr. 1991 Jan 18;538(1):3-25
2050794