Comparative assessment of selenite (SeIV) detoxification to elemental selenium (Se0) by Bacillus sp.
Bacillus licheniformis, B. subtilis, B. cereus, Bacillus pumilus and Exiguobacterium sp., which were resistant up to 20 mg Na(2)SeO(3)/ml in nutrient broth and 40 mg/ml on nutrient agar plates, were isolated from contaminated soil and water. They grew from 25 to 45 degrees C and pH 5 to 9. They had multiple metal and antibiotic resistances. All strains reduced selenite (SeIV) to elemental selenium (Se0) aerobically with a maximum reduction of 97% by B. pumilus after 144 h with Na(2)SeO(3) at 500 microg/ml.
Ikram
Madiha
M
Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore 54590, Pakistan.
Faisal
Muhammad
M
D000332:Aerobiosis; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D001407:Bacillus / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004783:Environmental Microbiology; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012643:Selenium / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D018038:Sodium Selenite / Q000378:metabolism* / Q000494:pharmacology; D013696:Temperature
D000900:Anti-Bacterial Agents; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012643:Selenium; D018038:Sodium Selenite