title: New stepwise cooling system for short-term porcine islet preservation.
abstract: OBJECTIVE
  Porcine islets are the most suitable for xeno-islet transplantation. However, it is necessary to establish an effective preservation method against its fragility. Recently, we developed a new cooling and preservation (Keep and Fresh [KFC]; FUJIYA Co, Tokushima, Japan) system, which can maintain viability of hepatocyte. In this study, we examined the KFC for porcine islet preservation.
  
  
  METHODS
  Isolated porcine islets were preserved in CMRL 1066 culture media with bovine serum at 37°C, 22°C, and 4°C and KFC for 24, 48, and 72 hours. Islet recovery rate, purity, and viability were evaluated.
  
  
  RESULTS
  After 24-hour preservation, the recovery rate was the highest in the KFC, but no significant difference was found. After 48-hour preservation, the recovery rate by the KFC was 73.9% ± 17.3%, which was significantly higher than the other groups (48.7% ± 28.6% at 37°C, P < 0.01; 46.6% ± 15.5% at 22°C, P < 0.01; 61.5% ± 20.0% at 4°C, P < 0.05). After 72-hour preservation, the difference of recovery rate was clearer. In the KFC group, purities and viabilities were the highest among the groups after 24-, 48-, and 72-hour preservation.
  
  
  CONCLUSIONS
  The KFC system significantly improved porcine islet preservation; therefore, the KFC might be useful for porcine islet preservation.
authors.affiliation: Baylor Institute for Immunology Research, Baylor Research Institute, Dallas, TX, USA.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ikemoto
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Noguchi
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Fujita
authors.forename: Morihito
authors.initials: M
authors.lastname: Takita
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimoda
authors.forename: Koji
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Andrew
authors.initials: A
authors.lastname: Jackson
authors.forename: Bashoo
authors.initials: B
authors.lastname: Naziruddin
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Shimada
authors.forename: Marlon F
authors.initials: MF
authors.lastname: Levy
authors.forename: Shinichi
authors.initials: S
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D002470:Cell Survival; D007515:Islets of Langerhans / Q000502:physiology*; D016381:Islets of Langerhans Transplantation; D009926:Organ Preservation / Q000379:methods*; D013552:Swine