New technology for rapid molecular diagnosis of bloodstream infections.
Technologies for the correct and timely diagnosis of bloodstream infections are urgently needed. Molecular diagnostic methods have yet to have a major impact on the diagnosis of bloodstream infections; however, new methods are being developed that are beginning to address key issues. In this article, we discuss the key needs and objectives of molecular diagnostics for bloodstream infections and review some of the currently available methods and how these techniques meet key needs. We then focus on a new method that combines nucleic acid amplification with mass spectrometry in a novel approach to molecular diagnosis of bloodstream infections.
Ecker
David J
DJ
Ibis Biosciences, a subsidiary of Abbott Molecular, Inc., 2251 Faraday Ave, Carlsbad, CA 92008, USA.
Sampath
Rangarajan
R
Li
Haijing
H
Massire
Christian
C
Matthews
Heather E
HE
Toleno
Donna
D
Hall
Thomas A
TA
Blyn
Lawrence B
LB
Eshoo
Mark W
MW
Ranken
Raymond
R
Hofstadler
Steven A
SA
Tang
Yi-Wei
YW
D000368:Aged; D016470:Bacteremia / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000097:blood*; D004271:DNA, Fungal / Q000097:blood*; D005260:Female; D016469:Fungemia / Q000175:diagnosis*; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation / Q000379:methods; D021141:Nucleic Acid Amplification Techniques; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D055815:Young Adult
D004269:DNA, Bacterial; D004271:DNA, Fungal