[Effect of epigallocatechin-3-gallate on the activity of alpha-glucosidase in vitro].
OBJECTIVE
To investigate the effect of epigallocatechin-3-gallate (EGCG) on the activity of alpha-glucosidase derived from bakers' yeast and small intestinal mucosa of Wistar rats, in order to establish an evaluation system for the activity of alpha-glucosidase inhibitor.


METHODS
alpha-glucosidase was prepared from the bakers' yeast and the small intestinal mucosa of Wistar rats. The inhibitory activity of EGCG on alpha-glucosidase was based on the concentration of glucose in the reaction system.


RESULTS
EGCG was shown to be an alpha-glucosidase inhibitor. IC50 was 0.150 mg/ml and 0.837 mg/ml for alpha-glucosidase derived from bakers' yeast and small intestinal mucosa respectively.


CONCLUSIONS
The establishment of an in vitro evaluation system for AGI activity is conductive for screening alpha-glucosidase inhibitory substance. The AGI activity of EGCG is strong in vitro.
Zhu
Yuming
Y
Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China. woshizhuyuming@163.com
Ding
Hong
H
Wang
Zhu
Z
Yang
Yuexin
Y
Xiang
Xuesong
X
D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D065089:Glycoside Hydrolase Inhibitors; D007413:Intestinal Mucosa / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D015003:Yeasts / Q000201:enzymology; D000520:alpha-Glucosidases / Q000378:metabolism*
D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D002392:Catechin; C045651:epigallocatechin gallate; D000520:alpha-Glucosidases