Ultrasound-array-based real-time photoacoustic microscopy of human pulsatile dynamics in vivo.
With a refined ultrasound-array-based real-time photoacoustic microscopy (UA-PAM) system, we demonstrate the feasibility of noninvasive in vivo imaging of human pulsatile dynamics. The system, capable of real-time B-scan imaging at 50 Hz and high-speed 3-D imaging, is validated by imaging the subcutaneous microvasculature in rats and humans. After the validation, a human artery around the palm-wrist area is imaged, and its pulsatile dynamics, including the arterial pulsatile motion and changes in hemoglobin concentration, is monitored with 20-ms B-scan imaging temporal resolution. To our knowledge, this is the first demonstration of real-time photoacoustic imaging of human physiological dynamics. Our results show that UA-PAM can potentially enable many new possibilities for studying functional and physiological dynamics in both preclinical and clinical imaging settings.
Song
Liang
L
Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130, USA.
Maslov
Konstantin
K
Shung
K Kirk
KK
Wang
Lihong V
LV
D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D008833:Microcirculation / Q000502:physiology*; D033401:Microscopy, Acoustic / Q000295:instrumentation*; D011673:Pulsatile Flow / Q000502:physiology*; D051381:Rats; D015203:Reproducibility of Results; D012212:Rheology / Q000295:instrumentation*; D012680:Sensitivity and Specificity
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