title: Aflatoxin M1 contamination and antibiotic residue in milk in Khorasan province, Iran.
abstract: The aim of this study was to evaluate aflatoxin M(1) (AFM(1)) contamination and antibiotic presence in milk samples in Khorasan province in Iran. During 4 months (March to June 2008), one hundred ninety-six milk samples were collected from seven dairies. The occurrence and concentration range of AFM(1) in the samples were investigated by competitive enzyme-linked immunoabsorbent assay (ELISA) method. Antibiotic presence was determined using Copan test, a broad-spectrum test capable of detecting beta-lactams, tetracyclines, sulfonamides, aminoglycosides and macrolydes. AFM(1) was found in 100% of the examined milk samples by average concentration of 77.92 ng/kg. The concentration of AFM(1) in all of the samples were lower than Iranian national standard and FDA limit (500 ng/L), but 80.6% of the samples had AFM(1) greater than the maximum tolerance limit (50 ng/L) accepted by European Union and Codex Alimentarius Commission. Statistical evaluation showed no significant difference between the mean concentrations of AFM(1) of milk samples taken from different factories (P>0.05). Copan milk test was positive for 40.8% of the samples.
authors.affiliation: Food Science and Technology Department, Islamic Azad University-Quchan Branch, Khorasan Province, Iran. mohamadisani@yahoo.com
authors.forename: A
authors.initials: A
authors.lastname: Mohamadi Sani
authors.forename: H
authors.initials: H
authors.lastname: Nikpooyan
authors.forename: R
authors.initials: R
authors.lastname: Moshiri
mesh_terms: D016607:Aflatoxin M1 / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002138:Calibration; D002417:Cattle; D004350:Drug Residues / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis*; D007202:Indicators and Reagents; D007492:Iran; D008892:Milk / Q000737:chemistry*; D013242:Sterilization
chemical_list: D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D016607:Aflatoxin M1