title: Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
abstract: We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeast--the only other eukaryote for which a comprehensive knockout library exists--revealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Dong-Uk
authors.initials: DU
authors.lastname: Kim
authors.affiliation: Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Hayles
authors.affiliation: Department of Bio and Brain Engineering, Korea Advanced Institute of Science & Technology (KAIST), Yuseong, Daejeon, Korea.
authors.forename: Dongsup
authors.initials: D
authors.lastname: Kim
authors.affiliation: Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.|Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1HH, UK.
authors.forename: Valerie
authors.initials: V
authors.lastname: Wood
authors.affiliation: Bioneer Corporation, Daedeok, Daejeon, Korea.
authors.forename: Han-Oh
authors.initials: HO
authors.lastname: Park
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Misun
authors.initials: M
authors.lastname: Won
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Hyang-Sook
authors.initials: HS
authors.lastname: Yoo
authors.affiliation: Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.
authors.forename: Trevor
authors.initials: T
authors.lastname: Duhig
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Miyoung
authors.initials: M
authors.lastname: Nam
authors.affiliation: Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.
authors.forename: Georgia
authors.initials: G
authors.lastname: Palmer
authors.affiliation: Department of Bio and Brain Engineering, Korea Advanced Institute of Science & Technology (KAIST), Yuseong, Daejeon, Korea.
authors.forename: Sangjo
authors.initials: S
authors.lastname: Han
authors.affiliation: Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.
authors.forename: Linda
authors.initials: L
authors.lastname: Jeffery
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Seung-Tae
authors.initials: ST
authors.lastname: Baek
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Hyemi
authors.initials: H
authors.lastname: Lee
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Young Sam
authors.initials: YS
authors.lastname: Shim
authors.affiliation: Department of Bio and Brain Engineering, Korea Advanced Institute of Science & Technology (KAIST), Yuseong, Daejeon, Korea.
authors.forename: Minho
authors.initials: M
authors.lastname: Lee
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Lila
authors.initials: L
authors.lastname: Kim
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Kyung-Sun
authors.initials: KS
authors.lastname: Heo
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Noh
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Ah-Reum
authors.initials: AR
authors.lastname: Lee
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Jang
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Kyung-Sook
authors.initials: KS
authors.lastname: Chung
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Shin-Jung
authors.initials: SJ
authors.lastname: Choi
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Jo-Young
authors.initials: JY
authors.lastname: Park
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Youngwoo
authors.initials: Y
authors.lastname: Park
authors.affiliation: Bioevaluation Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang, Chungcheongbuk-do, Korea.
authors.forename: Hwan Mook
authors.initials: HM
authors.lastname: Kim
authors.affiliation: Bioevaluation Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang, Chungcheongbuk-do, Korea.
authors.forename: Song-Kyu
authors.initials: SK
authors.lastname: Park
authors.affiliation: Bioneer Corporation, Daedeok, Daejeon, Korea.
authors.forename: Hae-Joon
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Bioneer Corporation, Daedeok, Daejeon, Korea.
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Kang
authors.affiliation: Department of Bioinformatics & Biotechnology, Korea University, Jochiwon, Chungnam, Korea.
authors.forename: Hyong Bai
authors.initials: HB
authors.lastname: Kim
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, Korea.
authors.forename: Hyun-Sam
authors.initials: HS
authors.lastname: Kang
authors.affiliation: Department of Microbiology, Chungnam National University, Yuseong, Daejeon, Korea.
authors.forename: Hee-Moon
authors.initials: HM
authors.lastname: Park
authors.affiliation: Division of Life Sciences, Kangwon National University, Chuncheon, Kangwon-do, Korea.
authors.forename: Kyunghoon
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Yonsei University, Seoul, Korea.
authors.forename: Kiwon
authors.initials: K
authors.lastname: Song
authors.affiliation: Department of Food and Nutrition, Chungnam National University, Yuseong, Daejeon, Korea.
authors.forename: Kyung Bin
authors.initials: KB
authors.lastname: Song
authors.affiliation: Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.|The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Nurse
authors.affiliation: Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.|Bioevaluation Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang, Chungcheongbuk-do, Korea.
authors.forename: Kwang-Lae
authors.initials: KL
authors.lastname: Hoe
mesh_terms: D004171:Diploidy; D017353:Gene Deletion; D020043:Genes, Essential / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics; D016681:Genome, Fungal / Q000235:genetics*; D006238:Haploidy; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
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