title: Distinct regulatory mechanism of immunoglobulin gene transcription in epithelial cancer cells.
abstract: The restriction of immunoglobulin (Ig) expression to B lymphocytes is well established. However, several reports have confirmed that the Ig gene can be expressed in many non-B cancer cells and/or some normal cells. Our aim is to determine whether the Ig gene promoter can be activated in non-B cancer cells and to identify the regulatory mechanism for Ig gene expression. Our results show that the Ig promoter of VH4-59 was activated in several non-B cancer cell lines. Moreover, two novel positive regulatory elements, an enhancer-like element at -800 to -610 bp and a copromoter-like element at -610 to -300 bp, were identified in two epithelial cancer cell lines, HeLa S3 and HT-29. The octamer element (5'-ATGCAAAT-3') located in the Ig promoter, a crucial element for B-cell-derived Ig gene transcription, was also very important for non-B-cell-derived Ig gene transcription. More importantly, we confirmed that octamer-related protein-1 (Oct-1), but not Oct-2, was a crucial transcriptional factor for Ig gene transcription due to its ability to bind to the octamer element of the Ig promoter in epithelial cancer cells. These results suggested the presence of a distinct regulatory mechanism for Ig gene expression in non-B cancer cells.
authors.affiliation: Peking University Center for Human Disease Genomics, Beijing, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhu
authors.forename: Lina
authors.initials: L
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Hao
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Yinan
authors.initials: Y
authors.lastname: Liu
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhou
authors.forename: Youhui
authors.initials: Y
authors.lastname: Zhang
authors.forename: C Cameron
authors.initials: CC
authors.lastname: Yin
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qiu
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D047369:Chromatin Immunoprecipitation; D024202:Electrophoretic Mobility Shift Assay; D004847:Epithelial Cells / Q000276:immunology* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D005803:Genes, Immunoglobulin / Q000235:genetics*; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000235:genetics* / Q000276:immunology*; D050811:Octamer Transcription Factor-1 / Q000378:metabolism; D050813:Octamer Transcription Factor-2 / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D014158:Transcription, Genetic
chemical_list: D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; C494165:POU2F1 protein, human; C058435:POU2F2 protein, human
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