Heat shock cognate protein 70 gene is required for prevention of apoptosis induced by WSSV infection.
Here, we show that heat shock cognate protein 70 (Hsc70) in shrimp cells can inhibit apoptosis induced by white spot syndrome virus (WSSV) infection. Caspase-3 protease activity of hemocytes increased significantly, correlating with a reduction in endogenous Hsc70 late in WSSV infection. Hsc70 dsRNA caused a significant increase in caspase-3 activity in the hemocytes of non-infected shrimp and WSSV-infected shrimp. We propose that upregulation of Hsc70 expression early in WSSV infection may also be used to prevent premature apoptotic cell death, and the precipitous downregulation of Hsc70 late in WSSV infection may lead to the timed induction of apoptosis.
Yan
Feng
F
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, People's Republic of China.
Xia
Ding
D
Hu
Jia
J
Yuan
Huixing
H
Zou
Tingting
T
Zhou
Qing
Q
Liang
Lihua
L
Qi
Yipeng
Y
Xu
Hua
H
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001562:Benzimidazoles; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D020868:Gene Silencing; D050883:HSC70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006434:Hemocytes / Q000821:virology; D006458:Hemolymph; D046849:Nimaviridae / Q000235:genetics / Q000378:metabolism*; D033561:Penaeidae / Q000166:cytology; D012367:RNA, Viral; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D001562:Benzimidazoles; D050883:HSC70 Heat-Shock Proteins; D012367:RNA, Viral; D014764:Viral Proteins; D053148:Caspase 3; C017807:bisbenzimide ethoxide trihydrochloride