Clock gene expression in gravid uterus and extra-embryonic tissues during late gestation in the mouse.
Evidence in humans and rodents suggests the importance of circadian rhythmicity in parturition. A molecular clock underlies the generation of circadian rhythmicity. While this molecular clock has been identified in numerous tissues, the expression and regulation of clock genes in tissues relevant to parturition is largely undefined. Here, the expression and regulation of the clock genes Bmal1, Clock, cryptochrome (Cry1/2) and period (Per1/2) was examined in the murine gravid uterus, placenta and fetal membranes during late gestation. All clock genes examined were expressed in the tissues of interest throughout the last third of gestation. Upregulation of a subset of these clock genes was observed in each of these tissues in the final two days of gestation. Oscillating expression of mRNA for a subset of the examined clock genes was detected in the gravid uterus, placenta and fetal membranes. Furthermore, bioluminescence recording on explants from gravid Per2::luciferase mice indicated rhythmic expression of PER2 protein in these tissues. These data demonstrate expression and rhythmicity of clock genes in tissues relevant to parturition indicating a potential contribution of peripheral molecular clocks to this process.
Ratajczak
Christine K
CK
Molecular Cell Biology Program, Washington University, St Louis, MO 63110, USA.
Herzog
Erik D
ED
Muglia
Louis J
LJ
D056930:ARNTL Transcription Factors / Q000235:genetics; D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics; D002940:Circadian Rhythm / Q000235:genetics*; D056925:Circadian Rhythm Signaling Peptides and Proteins / Q000235:genetics*; D056931:Cryptochromes / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D005865:Gestational Age; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D036801:Parturition / Q000235:genetics / Q000502:physiology*; D056950:Period Circadian Proteins / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D014599:Uterus / Q000737:chemistry*
D056930:ARNTL Transcription Factors; C538961:Bmal1 protein, mouse; D056925:Circadian Rhythm Signaling Peptides and Proteins; C538964:Cry1 protein, mouse; C538967:Cry2 protein, mouse; D056931:Cryptochromes; C539023:Per1 protein, mouse; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D056926:CLOCK Proteins; C538944:Clock protein, mouse
Curr Biol. 2004 Aug 10;14(15):1367-73
15296754
J Neurosci. 2006 Jul 5;26(27):7201-11
16822977
Reprod Fertil Dev. 2009;21(1):1-9
19152739
J Neurosci. 2003 Dec 3;23(35):11202-13
14657179
Reprod Fertil Dev. 2004;16(8):801-10
15740704
Endocrinology. 2009 Apr;150(4):1879-85
19056819
Occup Med (Lond). 2003 Mar;53(2):103-8
12637594
Proc Natl Acad Sci U S A. 2004 Apr 13;101(15):5339-46
14963227
Cell. 2007 Aug 24;130(4):730-741
17719549
Hum Reprod. 2006 Jan;21(1):68-79
16210390
Nat Genet. 2006 Mar;38(3):369-74
16474407
J Biol Rhythms. 2005 Oct;20(5):404-18
16267380
Biol Reprod. 2006 Oct;75(4):624-32
16807384
Eur J Neurosci. 2001 Feb;13(4):687-93
11207804
J Biol Rhythms. 2008 Feb;23(1):26-36
18258755
Mol Hum Reprod. 2003 Sep;9(9):503-7
12900508
Brain Res. 2008 Feb 21;1195:20-7
18201688
Science. 2006 Nov 24;314(5803):1304-8
17124323
Nature. 2002 Aug 29;418(6901):935-41
12198538
Methods. 2001 Dec;25(4):402-8
11846609
Genes Cells. 2006 May;11(5):525-30
16629904
J Comp Neurol. 2000 Aug 14;424(1):86-98
10888741
Cell. 2006 Aug 25;126(4):801-10
16923398
Brain Res. 1987 Feb 17;403(2):398-402
3828831
J Biol Chem. 2005 Dec 23;280(51):42036-43
16249183
Mol Imaging. 2007 Jan-Feb;6(1):68-72
17311766
J Neurosci. 2002 Jan 1;22(1):350-6
11756518