title: Polyphenolic constituents of methanolic and aqueous extracts of Vitex negundo render protection to Hep3B cells against oxidative cytotoxicity.
abstract: This study was aimed at investigating the phenolic constituents of methanolic and aqueous extracts of Vitex negundo in context of antioxidant potential and inhibition of oxidative stress-induced cytotoxicity. Antioxidant efficacies of both extracts were estimated by their abilities to scavenge DPPH(*) and to reduce Fe(+3) to Fe(+2). Their protective potential against H(2)O(2)-driven oxidative damage to cells were evaluated by XTT assay in Hep3B (human hepatoma) cells. Total phenolic content of both extracts were estimated, and were tested for correlation with antioxidant potential and protection against oxidative cytotoxicity. Polyphenolic levels in both extracts showed significant (P<0.05) positive correlations with DPPH(*) scavenging, Fe(+3)-Fe(+2) reduction and percentage decrease in H(2)O(2)-induced cytotoxicity. Major phenolic compounds in the extracts were identified by HPLC. It was concluded that both V. negundo extracts hold considerable potential as antitheses of free radical toxicity by virtue of their polyphenolic constituents, and might have significant clinical roles in prospect.
authors.affiliation: Division of Biomedical Sciences, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu 632 014, India.
authors.forename: Gunjan
authors.initials: G
authors.lastname: Guha
authors.forename: V
authors.initials: V
authors.lastname: Rajkumar
authors.forename: R
authors.initials: R
authors.lastname: Ashok Kumar
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005290:Ferric Compounds / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D007202:Indicators and Reagents; D015227:Lipid Peroxidation / Q000187:drug effects; D000432:Methanol; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000032:analysis* / Q000494:pharmacology*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D059808:Polyphenols; D020011:Protective Agents / Q000032:analysis* / Q000494:pharmacology*; D012997:Solvents; D013451:Sulfonic Acids / Q000737:chemistry; D020950:Vitex / Q000737:chemistry*; D014867:Water
chemical_list: D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005290:Ferric Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D020011:Protective Agents; D012997:Solvents; D013451:Sulfonic Acids; D014867:Water; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol