Formation of virus-like particles from O-type foot-and-mouth disease virus in insect cells using codon-optimized synthetic genes.
A recombinant baculovirus was constructed to simultaneously express codon-optimized virus-like particles (VLP), A VP1-2A-VP3 and VP0 of serotype O foot-and-mouth disease virus (FMDV), from individual promoters. The target proteins were expressed in insect cells at high level, as shown by indirect sandwich ELISA; and the expressed VP1-2A-VP3 could autocatalytically be cleaved into the individual proteins, VP1-2A and VP3, as shown by Western-blot analyses. In addition, in the insect cells, the structural proteins, VP0, VP3 and VP1-2A, self-assembled into virus-like particles resembling the authentic FMDV particles. This information should prove useful for the development of more efficient VLP assembly using shorter genes.
Cao
Yimei
Y
Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping No. 1, Yanchangpu, Lanzhou 730046, Gansu, The People's Republic of China.
Sun
Pu
P
Fu
Yuanfang
Y
Bai
Xingwen
X
Tian
Feipen
F
Liu
Xiangtao
X
Lu
Zengjun
Z
Liu
Zaixin
Z
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D002460:Cell Line; D003062:Codon / Q000235:genetics; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics*; D005813:Genes, Synthetic; D005814:Genes, Viral; D005822:Genetic Vectors; D007313:Insecta; D011485:Protein Binding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics; D022701:Virosomes / Q000096:biosynthesis*; D019065:Virus Assembly
D003062:Codon; D011994:Recombinant Proteins; D014764:Viral Proteins; D022701:Virosomes