Pre-analytical in-vitro stability of [-2]proPSA in blood and serum.
OBJECTIVE
[-2]proPSA may discriminate prostate cancer from benign biopsy results. We characterized the pre-analytical stability of [-2]proPSA.


METHODS
22 volunteers, total PSA of 4.5-19.3microg/L, had blood drawn simultaneously. Baseline measurements were performed and samples were stored under various conditions prior to measurements. Freeze-thaw cycles were performed. [-2]proPSA was measured with the p2PSA automated research use only immunoassay on the Access analyzer.


RESULTS
Mean [-2]proPSA increases with clotting time, exceeding 10% change in recovery after 3h. In serum, [-2]proPSA values decline over time under investigated storage conditions. Serum samples kept frozen show less than 10% variation in recoveries over the course of 2 freeze-thaw cycles.


CONCLUSIONS
For proper measurement of [-2]proPSA, blood samples should be centrifuged within 3h of blood draw. Serum may be stored at RT or refrigerated (+4 degrees C) for a maximum of 48h and should be frozen if stored for a longer period. Two freeze-thaw cycles have no effect on [-2]proPSA stability.
Semjonow
Axel
A
Dept. of Urology, Prostate Center, University Clinic Münster, Albert-Schweitzer-Strasse 33, D-48149 Münster, Germany. semjono@uni-muenster.de
Köpke
Thomas
T
Eltze
Elke
E
Pepping-Schefers
Beate
B
Bürgel
Hilla
H
Darte
Claude
C
D001793:Blood Preservation; D001800:Blood Specimen Collection; D006801:Humans; D007118:Immunoassay; D016014:Linear Models; D008297:Male; D011237:Predictive Value of Tests; D017430:Prostate-Specific Antigen / Q000097:blood*; D012016:Reference Values
D017430:Prostate-Specific Antigen