mRNA profiling for the identification of blood--results of a collaborative EDNAP exercise.
A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, using different kits, chemistries and instrumentation. The results demonstrate that HBB is expressed abundantly in blood, SPTB moderately and PBGD significantly less. All but one of the 16 participating laboratories were able to successfully isolate and detect RNA from the dried bloodstains even though a majority of the laboratories had no prior experience with RNA. Despite some expected variation in sensitivity between laboratories, the method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise support the potential use of mRNA profiling as an alternative to conventional serological tests.
Haas
C
C
Institute of Legal Medicine, University of Zürich, Zürich, Switzerland. cordula.haas@irm.uzh.ch
Hanson
E
E
Bär
W
W
Banemann
R
R
Bento
A M
AM
Berti
A
A
Borges
E
E
Bouakaze
C
C
Carracedo
A
A
Carvalho
M
M
Choma
A
A
Dötsch
M
M
Durianciková
M
M
Hoff-Olsen
P
P
Hohoff
C
C
Johansen
P
P
Lindenbergh
P A
PA
Loddenkötter
B
B
Ludes
B
B
Maroñas
O
O
Morling
N
N
Niederstätter
H
H
Parson
W
W
Patel
G
G
Popielarz
C
C
Salata
E
E
Schneider
P M
PM
Sijen
T
T
Sviezená
B
B
Zatkalíková
L
L
Ballantyne
J
J
D015415:Biomarkers / Q000097:blood; D001801:Blood Stains; D003299:Cooperative Behavior; D016172:DNA Fingerprinting / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary; D006801:Humans; D011163:Hydroxymethylbilane Synthase / Q000032:analysis; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques; D012313:RNA / Q000097:blood / Q000302:isolation & purification; D012333:RNA, Messenger / Q000097:blood* / Q000737:chemistry; D011933:Reagent Kits, Diagnostic / Q000706:statistics & numerical data; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013049:Spectrin / Q000032:analysis; D044465:White People / Q000235:genetics*; D055544:beta-Globins / Q000032:analysis
D015415:Biomarkers; D012333:RNA, Messenger; D011933:Reagent Kits, Diagnostic; D055544:beta-Globins; D013049:Spectrin; D012313:RNA; D011163:Hydroxymethylbilane Synthase