title: Drosophila indirect flight muscle specific Act88F actin mutants as a model system for studying congenital myopathies of the human ACTA1 skeletal muscle actin gene.
abstract: Most human ACTA1 skeletal actin gene mutations cause dominant, congenital myopathies often with severely reduced muscle function and neonatal mortality. High sequence conservation of actin means many mutated ACTA1 residues are identical to those in the DrosophilaAct88F, an indirect flight muscle specific sarcomeric actin. Four known Act88F mutations occur at the same actin residues mutated in ten ACTA1 nemaline mutations, A138D/P, R256H/L, G268C/D/R/S and R372C/S. These Act88F mutants were examined for similar muscle phenotypes. Mutant homozygotes show phenotypes ranging from a lack of myofibrils to almost normal sarcomeres at eclosion. Aberrant Z-disc-like structures and serial Z-disc arrays, 'zebra bodies', are observed in homozygotes and heterozygotes of all four Act88F mutants. These electron-dense structures show homologies to human nemaline bodies/rods, but are much smaller than those typically found in the human myopathy. We conclude that the Drosophila indirect flight muscles provide a good model system for studying ACTA1 mutations.
authors.affiliation: Department of Biology, University of York, York YO10 5DD, UK.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Haigh
authors.forename: Sheetal S
authors.initials: SS
authors.lastname: Salvi
authors.forename: Maria
authors.initials: M
authors.lastname: Sevdali
authors.forename: Meg
authors.initials: M
authors.lastname: Stark
authors.forename: David
authors.initials: D
authors.lastname: Goulding
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Clayton
authors.forename: Belinda
authors.initials: B
authors.lastname: Bullard
authors.forename: John C
authors.initials: JC
authors.lastname: Sparrow
authors.forename: Upendra
authors.initials: U
authors.lastname: Nongthomba
mesh_terms: D000199:Actins / Q000235:genetics* / Q000502:physiology; D000818:Animals; D015153:Blotting, Western; D004247:DNA / Q000235:genetics; D004330:Drosophila / Q000502:physiology*; D005426:Flight, Animal / Q000502:physiology*; D006801:Humans; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008958:Models, Molecular; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D009132:Muscles / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D009135:Muscular Diseases / Q000151:congenital / Q000235:genetics* / Q000473:pathology; D009154:Mutation; D009210:Myofibrils / Q000473:pathology; D011679:Pupa
chemical_list: D000199:Actins; D004247:DNA