Agrobacterium-mediated transformation of Euphorbia tirucalli callus.
In order to establish a basis for transformation technology in the petroleum plant Euphorbia tirucalli, the callus of the plant was infected with Agrobacterium, washed with distilled water, sterilized with distilled water containing 100 mg/l of carbenicillin, selected on solidified B5 medium containing 13 mg/l of G418 and 100 mg/l of carbenicillin, and then on solidified B5 medium containing 25 mg/l of G418 and 100 mg/l of carbenicillin for the transgenic calli, and then the callus lines were subcultured successively on solidified B5 medium containing 50 mg/l of G418. We performed PCR analysis of sterilized G418-resistant callus line DNA and concluded that the gene introduced was integrated into the callus genome.
Uchida
Hidenobu
H
School of Environmental Science and Engineering, Kochi University of Technology, Japan. uchida.hidenobu@kochi-tech.ac.jp
Yamashita
Hirofumi
H
Anai
Toyoaki
T
Muranaka
Toshiya
T
Ohyama
Kanji
K
D002228:Carbenicillin / Q000494:pharmacology; D028481:Euphorbia / Q000235:genetics; D005786:Gene Expression Regulation; D005839:Gentamicins; D012231:Rhizobium / Q000235:genetics*; D014162:Transfection / Q000379:methods*
D005839:Gentamicins; C010680:antibiotic G 418; D002228:Carbenicillin