Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice.
Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 5' and 3' alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.
Llères
David
D
Wellcome Trust Centre for Gene Regulation & Expression, College of Life Sciences, University of Dundee, MSI/WTB/JBC Complex, Dow Street, Dundee DD1 5EH, UK.
Denegri
Marco
M
Biggiogera
Marco
M
Ajuh
Paul
P
Lamond
Angus I
AI
D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D018869:Heat-Shock Response; D034761:Heterogeneous-Nuclear Ribonucleoprotein Group M / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000378:metabolism*; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D022821:RNA Splice Sites; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins
C508357:CDC5L protein, human; D018797:Cell Cycle Proteins; D034761:Heterogeneous-Nuclear Ribonucleoprotein Group M; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; C479410:PLRG1 protein, human; D022821:RNA Splice Sites; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
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