Herpes simplex virus type 1 entry into epithelial MDCKII cells: role of VASP activities.
VASP is an actin-regulatory protein that links signalling to remodelling of the cytoskeleton. We investigated the role of VASP during entry of herpes simplex viruses into epithelial MDCKII cells. As VASP functions are regulated by phosphorylations, the phosphorylation pattern was determined upon infection. Phosphorylated VASP decreased temporarily at 15 and 30 min after infection. The impact of phosphorylated VASP was addressed by overexpression of phosphomimetic VASP mutants. Our results revealed that phosphorylated VASP slightly reduced the number of infected cells. Expression studies with deletion mutants further indicated minor effects of VASP on infection efficiency, whereas RNA interference studies demonstrated that reduced VASP expression did not suppress infection. We conclude that VASP activities alone may contribute to herpes simplex virus infection to only a minor extent.
Jaeger
Verena
V
Max-Planck-Institute for Neurological Research, D-50931 Cologne, Germany.
Hoppe
Sven
S
Petermann
Philipp
P
Liebig
Timo
T
Jansen
Matthias K
MK
Renné
Thomas
T
Knebel-Mörsdorf
Dagmar
D
D019943:Amino Acid Substitution; D000818:Animals; D015815:Cell Adhesion Molecules / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D004285:Dogs; D017353:Gene Deletion; D015870:Gene Expression; D006561:Herpes Simplex / Q000209:etiology / Q000503:physiopathology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000472:pathogenicity* / Q000502:physiology*; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010750:Phosphoproteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D010766:Phosphorylation; D034622:RNA Interference; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012694:Serine / Q000737:chemistry; D014162:Transfection; D053586:Virus Internalization; D000098662:Vasodilator-Stimulated Phosphoprotein
D015815:Cell Adhesion Molecules; D008840:Microfilament Proteins; D010750:Phosphoproteins; D011994:Recombinant Proteins; D000098662:Vasodilator-Stimulated Phosphoprotein; D012694:Serine