The effect of acetaminophen nanoparticles on liver toxicity in a rat model.
Acetaminophen, a pain-reliever, is one of the most widely used medications in the world. Acetaminophen with normal dosage is considered a nontoxic drug for therapeutic applications, but when taken at overdose levels it produces liver damage in human and various animal species. By a high energy mechanically activated method, we produced acetaminophen in a nanometer crystalline size (24 nm). Forty-eight hours after injection of crystalline particles with normal and reduced size of our drug, the effect of liver toxicity was compared by determination of liver transferase enzymes such as alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase (ALT). These enzymes were examined by routine colorimetric methods using commercial kits and pathologic investigations. Statistical analysis and pathological figures indicated that ALT delivery and toxicity in reduced size acetaminophen was significantly reduced when compared with normal size acetaminophen. Pathology figures exhibited reduced necrosis effects, especially the confluent necrosis, in the central part of the lobule in the reduced size acetaminophen samples when compared with the normal samples.
Biazar
Esmaeil
E
Department of Chemistry, Islamic Azad University, Tonekabon Branch, Mazandaran, Iran. e.biazar@tonekaboniau.ac.ir
Rezayat
S Mahdi
SM
Montazeri
Naser
N
Pourshamsian
Khalil
K
Zeinali
Reza
R
Asefnejad
Azadeh
A
Rahimi
Mehdi
M
Zadehzare
Mohammadmajid
M
Mahmoudi
Mehran
M
Mazinani
Rohollah
R
Ziaei
Mehdi
M
D000082:Acetaminophen / Q000633:toxicity*; D018712:Analgesics, Non-Narcotic / Q000633:toxicity; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology* / Q000473:pathology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000473:pathology*; D053758:Nanoparticles / Q000633:toxicity*; D051381:Rats
D018712:Analgesics, Non-Narcotic; D000082:Acetaminophen
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