Comparative studies on lectin-carbohydrate interactions in low and high density homo- and heteroglycoclusters.
A versatile synthetic procedure to construct series of high- and low-density homo- and heteroglycoclusters is reported. The binding properties of these synthetic multivalent glycoconjugates to concanavalin A (Con A), a model lectin, have been assessed by using a range of competitive and non-competitive binding assays including enzyme-linked lectin assays (ELLA), isothermal titration microcalorimetry (ITC) and surface plasmon resonance (SPR). In all cases, highly dense glycoclusters showed a substantial amplification of the lectin-binding strength in comparison with low-density counterparts. Interestingly, highly-dense glycoligand presentations, regardless of their homo- or heteroglycoligand pattern, furnished similar Con A binding properties, supporting the existence of a synergic effect (heterocluster effect) due to secondary interactions of "non-active" structural motifs in the presence of a certain density of "active" glycoligands.
Gómez-García
Marta
M
Departamento de Química Orgánica, Facultad de Química, Universidad de Sevilla, Apartado 1203, E-41012 Sevilla, Spain.
Benito
Juan M
JM
Gutiérrez-Gallego
Ricardo
R
Maestre
Alfredo
A
Mellet
Carmen Ortiz
CO
Fernández
José M García
JM
Blanco
José L Jiménez
JL
D001665:Binding Sites; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000138:chemical synthesis / Q000737:chemistry; D003208:Concanavalin A / Q000378:metabolism*; D057075:Enzyme Assays; D006001:Glycoconjugates / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020349:Surface Plasmon Resonance
D002241:Carbohydrates; D006001:Glycoconjugates; D037102:Lectins; D003208:Concanavalin A