title: Characterization of the transcriptome of chorioamniotic membranes at the site of rupture in spontaneous labor at term.
abstract: OBJECTIVE
  The purpose of this study was to compare the transcriptome between the site of membrane rupture and the chorioamniotic membranes away from the site of rupture.
  
  
  METHODS
  The transcriptome of amnion and chorion (n=20 each) from and distal to the site of rupture from women with spontaneous labor and vaginal delivery at term after spontaneous rupture of membranes was profiled with Illumina HumanHT-12 microarrays. Selected genes were validated with the use of quantitative reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  Six hundred seventy-seven genes were differentially expressed in the chorion between the rupture and nonrupture sites (false discovery rate<0.1; fold change>1.5). Quantitative reverse transcription-polymerase chain reaction confirmed the differential expression in 10 of 14 genes. Enriched biological processes included anatomic structure development, cell adhesion and signal transduction. Extracellular matrix-receptor interaction was the most impacted signaling pathway.
  
  
  CONCLUSIONS
  The transcriptome of fetal membranes after spontaneous rupture of membranes in term labor is characterized by region- and tissue-specific differential expression of genes that are involved in signature pathways, which include extracellular matrix-receptor interactions.
authors.affiliation: Perinatology Research Branch, Intramural Division, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NICHD/NIH/DHHS, Bethesda, MD, USA.
authors.forename: Chia-Ling
authors.initials: CL
authors.lastname: Nhan-Chang
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero
authors.forename: Adi L
authors.initials: AL
authors.lastname: Tarca
authors.forename: Pooja
authors.initials: P
authors.lastname: Mittal
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Kusanovic
authors.forename: Offer
authors.initials: O
authors.lastname: Erez
authors.forename: Shali
authors.initials: S
authors.lastname: Mazaki-Tovi
authors.forename: Tinnakorn
authors.initials: T
authors.lastname: Chaiworapongsa
authors.forename: John
authors.initials: J
authors.lastname: Hotra
authors.forename: Nandor Gabor
authors.initials: NG
authors.lastname: Than
authors.forename: Jung-Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Sonia S
authors.initials: SS
authors.lastname: Hassan
authors.forename: Chong Jai
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000328:Adult; D000650:Amnion / Q000502:physiology; D002823:Chorion / Q000502:physiology; D005321:Extraembryonic Membranes / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007743:Labor, Obstetric / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D011446:Prospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D055815:Young Adult
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