title: A small nucleolar RNA functions in rRNA processing in Caenorhabditis elegans.
abstract: CeR-2 RNA is one of the newly identified Caenorhabditis elegans noncoding RNAs (ncRNAs). The characterization of CeR-2 by RNomic studies has failed to classify it into any known ncRNA family. In this study, we examined the spatiotemporal expression patterns of CeR-2 to gain insight into its function. CeR-2 is expressed in most cells from the early embryo to adult stages. The subcellular localization of this RNA is analogous to that of fibrillarin, a major protein of the nucleolus. It was observed that knockdown of C/D small nucleolar ribonucleoproteins (snoRNPs), but not of H/ACA snoRNPs, resulted in the aberrant nucleolar localization of CeR-2 RNA. A mutant worm with a reduced amount of cellular CeR-2 RNA showed changes in its pre-rRNA processing pattern compared with that of the wild-type strain N2. These results suggest that CeR-2 RNA is a C/D snoRNA involved in the processing of rRNAs.
authors.affiliation: Functional Genomics and Technology, United Graduate School of Agricultural Science, Iwate University, 18-8 Ueda 3-chome, Morioka 020-8550.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Hokii
authors.forename: Yumi
authors.initials: Y
authors.lastname: Sasano
authors.forename: Mayu
authors.initials: M
authors.lastname: Sato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakamoto
authors.forename: Kazumi
authors.initials: K
authors.lastname: Sakata
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Shingai
authors.forename: Akito
authors.initials: A
authors.lastname: Taneda
authors.forename: Shigenori
authors.initials: S
authors.lastname: Oka
authors.forename: Hyouta
authors.initials: H
authors.lastname: Himeno
authors.forename: Akira
authors.initials: A
authors.lastname: Muto
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Chisato
authors.initials: C
authors.lastname: Ushida
mesh_terms: D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012322:RNA Precursors / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000378:metabolism*; D020537:RNA, Small Nucleolar / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D020718:Ribonucleoproteins, Small Nucleolar / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D012322:RNA Precursors; D012335:RNA, Ribosomal; D020537:RNA, Small Nucleolar; D020718:Ribonucleoproteins, Small Nucleolar
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