Depressive-like-behavior and proinflamatory interleukine levels in the brain of rats submitted to pneumococcal meningitis.
Bacterial meningitis due to Streptococcus pneumoniae is associated with a significant mortality rate and persisting neurologic sequelae including sensorymotor deficits, seizures, and impairments of learning and memory. The presence of proliferating bacteria within the subarachnoid and ventricular space compartments triggers an intense inflammatory host response. Proinflammatory mediators released in the process include tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-1beta, IL-6, and all of which have been shown to contribute to the development of brain injury in bacterial meningitis. The animals underwent a magna cistern tap receiving either 10muL sterile saline as a placebo or an equivalent volume of a S. pneumoniae suspension at the concentration 5x10(9)cfu/mL. Ten days after induction we evaluated depressive-like behavior by using the forced swimming test and verified the levels of the TNF-alpha, IL-1beta, IL-6 and CINC-1 in the brain of rats induced to pneumococcal meningitis. In the forced swimming test we observed a significant increase in the immobility time in the meningitis group compared to the sham group (p<0.05). The TNFlevels were found increased in the prefrontal cortex (p<0.05, F=4.921), but not hippocampus. The IL-6, CINC-1 and IL-1beta levels presented no alteration in both prefrontal cortex and hippocampus 10 days after meningitis induction by S. pneumoniae. These findings suggest that the meningitis model could be a good research tool for the study of the biological mechanisms involved in the behavioral alterations secondary to pneumococcal meningitis.
Barichello
Tatiana
T
Laboratory of Experimental Microbiology and National Institute for Translational Medicine (INCT-TM), Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000 Criciúma, SC, Brazil. tba@unesc.net
Dos Santos
Ivonete
I
Savi
Geovana D
GD
Simões
Lutiana R
LR
Generoso
Jaqueline S
JS
Comim
Clarissa M
CM
Sachs
Daniela
D
Teixeira
Antonio L
AL
Quevedo
João
J
D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D054360:Chemokine CXCL1 / Q000378:metabolism; D003863:Depression / Q000209:etiology*; D004195:Disease Models, Animal; D005106:Exploratory Behavior / Q000502:physiology; D046353:Immobility Response, Tonic / Q000502:physiology; D007378:Interleukins / Q000378:metabolism*; D008297:Male; D008586:Meningitis, Pneumococcal / Q000150:complications / Q000276:immunology / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013550:Swimming / Q000523:psychology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D054360:Chemokine CXCL1; C513297:Cxcl1 protein, rat; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha