title: Transdermal drug delivery aided by an ultrasound contrast agent: an in vitro experimental study.
abstract: Sonophoresis temporarily increases skin permeability such that medicine can be delivered transdermally. Cavitation is believed to be the predominant mechanism in sonophoresis. In this study, an ultrasound contrast agent (UCA) strategy was adopted instead of low frequency ultrasound to assure that cavitation occurred, and the efficacy of sonophoresis with UCA was quantitatively analyzed by optical measurements. The target drug used in this study was 0.1 % Definity(R) in 70% glycerol, which was delivered into porcine skin samples. Glycerol was used because it is an optical clearing agent, and the efficiency of glycerol delivery could be analyzed with optical measurements. The applied acoustic pressure was approximately 600 kPa at 1 MHz ultrasound with a 10% duty cycle for 60 minutes. Experimental results indicated that the measured relative contrast (RC) after sonophoresis with UCA was approximately 80% higher than RC after sonophoresis without UCA. In addition, the variance of RC was also reduced by more than 50% with the addition of a UCA. The use of a UCA appeared to increase cavitation, demonstrating that the use of a UCA can be effective in transdermal drug delivery (TDD).
authors.affiliation: Department of Biomedical Engineering, Yonsei University, 234 Maeji, Heungup, Wonju, Kangwon, 220-710, S. Korea.
authors.forename: Donghee
authors.initials: D
authors.lastname: Park
authors.forename: Jinhee
authors.initials: J
authors.lastname: Yoon
authors.forename: Jingam
authors.initials: J
authors.lastname: Park
authors.forename: Byungjo
authors.initials: B
authors.lastname: Jung
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Park
authors.forename: Jongbum
authors.initials: J
authors.lastname: Seo
references.citation: Adv Drug Deliv Rev. 1999 Jan 4;35(1):61-76
references.pmid: 10837689
references.citation: Altern Lab Anim. 2006 Oct;34(5):499-513
references.pmid: 17121474
references.citation: J Control Release. 2006 Feb 21;110(3):557-65
references.pmid: 16356576
references.citation: J Control Release. 2000 Feb 14;64(1-3):129-32
references.pmid: 10640651
references.citation: Annu Rev Biomed Eng. 2007;9:415-47
references.pmid: 17651012
references.citation: Adv Drug Deliv Rev. 2004 Mar 27;56(5):659-74
references.pmid: 15019751
references.citation: Lasers Surg Med. 2010 Jul;42(5):412-7
references.pmid: 20583247
references.citation: Science. 1990 Sep 28;249(4976):1527-33
references.pmid: 2218494
references.citation: J Biomed Opt. 2006 Sep-Oct;11(5):054027
references.pmid: 17092176
references.citation: Radiology. 2001 Sep;220(3):640-6
references.pmid: 11526261
references.citation: Biochim Biophys Acta. 2004 Oct 11;1665(1-2):134-41
references.pmid: 15471579
references.citation: Adv Drug Deliv Rev. 1999 Apr 5;37(1-3):139-157
references.pmid: 10837732
references.citation: Ultrasound Med Biol. 2007 Jan;33(1):95-104
references.pmid: 17189051
references.citation: Adv Drug Deliv Rev. 2004 Mar 27;56(5):589-601
references.pmid: 15019748
references.citation: J Control Release. 2002 Nov 7;84(1-2):49-57
references.pmid: 12399167
references.citation: Crit Rev Ther Drug Carrier Syst. 1994;11(2-3):161-213
references.pmid: 7600587
references.citation: IEEE Trans Ultrason Ferroelectr Freq Control. 2003 Oct;50(10):1296-304
references.pmid: 14609069
references.citation: J Biomed Opt. 2008 Mar-Apr;13(2):021105
references.pmid: 18465954
references.citation: Adv Drug Deliv Rev. 1999 Jan 4;35(1):77-88
references.pmid: 10837690
references.citation: J Control Release. 2003 Jun 24;90(2):171-9
references.pmid: 12810300
references.citation: Pharm Res. 1996 Mar;13(3):411-20
references.pmid: 8692734
references.citation: Drug Discov Today. 2004 Aug 1;9(15):670-6
references.pmid: 15279850