Yaniella soli sp. nov., a new actinobacterium isolated from non-saline forest soil in China.
A novel Gram-stain-positive, slightly halophilic, facultatively alkaliphilic, non-motile, non-sporulating, catalase-positive, oxidase-negative, aerobic bacterium, designated strain JSM 070026(T), was isolated from non-saline forest soil in China. Growth occurred with 0-20% (w/v) NaCl (optimum, 2-4%) and at pH 6.0-10.5 (optimum, pH 8.0) and 5-40 degrees C (optimum, 30 degrees C). Good growth also occurred in the presence of 0-28% (w/v) KCl (optimum, 2-5%) or 0-25% (w/v) MgCl(2)*6H(2)O (optimum, 1-4%). The peptidoglycan type was A4alpha (L: -Lys-Gly-L: -Glu). Cell-wall sugars contained mannose and xylose. The major cellular fatty acids were anteiso-C15:0 and iso-C15:0. Strain JSM 070026(T) contained menaquinone 8 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major polar lipids. The DNA G + C content of strain JSM 070026(T) was 56.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JSM 070026(T) was a member of the suborder Micrococcineae and most closely related to Yaniella flava YIM 70178(T) (sequence similarity 99.4%) and Yaniella halotolerans YIM 70085(T) (97.9%). The three strains formed a distinct branch in the phylogenetic tree. The combination of phylogenetic analysis, DNA-DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the proposal that strain JSM 070026(T) represents a novel species of the genus Yaniella, for which the name Yaniella soli sp. nov. is proposed. The type strain is JSM 070026(T) (=DSM 22211(T) = KCTC 13527(T)).
Chen
Yi-Guang
YG
College of Biology and Environmental Sciences, Department of Financial Management, Jishou University, 416000 Jishou, People's Republic of China.
Chen
Jun
J
Chen
Qi-Hui
QH
Tang
Shu-Kun
SK
Zhang
Yu-Qin
YQ
He
Jian-Wu
JW
Li
Wen-Jun
WJ
Liu
Yan-Qi
YQ
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D020459:Genes, rRNA; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride