title: Herpes simplex virus type 1 entry into epithelial MDCKII cells: role of VASP activities.
abstract: VASP is an actin-regulatory protein that links signalling to remodelling of the cytoskeleton. We investigated the role of VASP during entry of herpes simplex viruses into epithelial MDCKII cells. As VASP functions are regulated by phosphorylations, the phosphorylation pattern was determined upon infection. Phosphorylated VASP decreased temporarily at 15 and 30 min after infection. The impact of phosphorylated VASP was addressed by overexpression of phosphomimetic VASP mutants. Our results revealed that phosphorylated VASP slightly reduced the number of infected cells. Expression studies with deletion mutants further indicated minor effects of VASP on infection efficiency, whereas RNA interference studies demonstrated that reduced VASP expression did not suppress infection. We conclude that VASP activities alone may contribute to herpes simplex virus infection to only a minor extent.
authors.affiliation: Max-Planck-Institute for Neurological Research, D-50931 Cologne, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Jaeger
authors.forename: Sven
authors.initials: S
authors.lastname: Hoppe
authors.forename: Philipp
authors.initials: P
authors.lastname: Petermann
authors.forename: Timo
authors.initials: T
authors.lastname: Liebig
authors.forename: Matthias K
authors.initials: MK
authors.lastname: Jansen
authors.forename: Thomas
authors.initials: T
authors.lastname: Renné
authors.forename: Dagmar
authors.initials: D
authors.lastname: Knebel-Mörsdorf
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D015815:Cell Adhesion Molecules / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D004285:Dogs; D017353:Gene Deletion; D015870:Gene Expression; D006561:Herpes Simplex / Q000209:etiology / Q000503:physiopathology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000472:pathogenicity* / Q000502:physiology*; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010750:Phosphoproteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D010766:Phosphorylation; D034622:RNA Interference; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012694:Serine / Q000737:chemistry; D014162:Transfection; D053586:Virus Internalization; D000098662:Vasodilator-Stimulated Phosphoprotein
chemical_list: D015815:Cell Adhesion Molecules; D008840:Microfilament Proteins; D010750:Phosphoproteins; D011994:Recombinant Proteins; D000098662:Vasodilator-Stimulated Phosphoprotein; D012694:Serine