A call to standardize teratoma assays used to define human pluripotent cell lines.
The teratoma assay is the gold standard for documenting pluripotency of human stem cells. However, reports of new human ESC and iPSC lines vary widely in both methods and analysis of teratoma data. We call for consensus standards to be established to make this assay worthy of its "golden" status.
Müller
Franz-Josef
FJ
Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA. fj.mueller@zip.kiel.de
Goldmann
Johanna
J
Löser
Peter
P
Loring
Jeanne F
JF
D000818:Animals; D001681:Biological Assay / Q000379:methods* / Q000592:standards*; D002460:Cell Line; D032921:Consensus; D006801:Humans; D015201:Meta-Analysis as Topic; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology*; D012015:Reference Standards; D013724:Teratoma / Q000473:pathology*