title: Comparison of the automated Phoenix with the Vitek 2 for the identification of Streptococcus pneumoniae.
abstract: Rapid and accurate identification of Streptococcus pneumoniae is a critical component in the optimal management of infected patients. The performance of the BD Phoenix Automated Microbiology System (BD Diagnostic Systems, Sparks, Md.) was evaluated for identification of S. pneumoniae (n = 311) and was compared to the Vitek 2 (bioMérieux, Marcy l'Etoile, France). Strains with discordant identification between methods were resolved with 16S rRNA gene sequencing as the gold standard. The Phoenix and the Vitek 2 correctly identified 96.8% (n = 301) and 95.2% (n = 296) of S. pneumoniae strains, respectively. Overall, there was no statistically significant difference in the performance of the 2 automated systems for the identification of S. pneumoniae in this study. The Vitek 2 mean time-to-results for all streptococcal identification was 1.5 h faster than that for the Phoenix. We conclude that the automated Phoenix and the Vitek 2 systems are comparable in their ability to identify S. pneumoniae and are preferable to the use of routine biochemical assays, which have delayed time-to-results and are not dependably accurate.
authors.affiliation: Clinical Microbiology Service, NewYork-Presbyterian Hospital, Columbia University Medical Center, New York, NY 10032, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Mittman
authors.forename: Richard C
authors.initials: RC
authors.lastname: Huard
authors.forename: Phyllis
authors.initials: P
authors.lastname: Della-Latta
authors.forename: Susan
authors.initials: S
authors.lastname: Whittier
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D006801:Humans; D011008:Pneumococcal Infections / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000302:isolation & purification*