Intragastric administration of a superoxide dismutase-producing recombinant Lactobacillus casei BL23 strain attenuates DSS colitis in mice.
Human immune cells release large amounts of reactive oxygen species (ROS) such as superoxide radical and hydrogen peroxide via respiratory burst. In inflammatory bowel diseases, a sustained and abnormal activation of the immune response results in oxidative stress of the digestive tract and in a loss of intestinal homeostasis. We previously reported that heterologous production of the Lactobacillus plantarum manganese catalase (MnKat) enhances the survival of Lb. casei BL23 when exposed to oxidative stress. Anti-inflammatory effects were observed after Lb. casei BL23 oral administrations in moderate murine dextran sodium sulfate (DSS)-induced colitis, without added effects of the MnKat production. Here, we evaluated the protective effects obtained by an improved antioxidative strategy. The Lactococcus lactis sodA gene was expressed in Lb. casei BL23 which acquired an efficient manganese superoxide dismutase (MnSOD) activity. The effects of Lb. casei MnSOD alone or in combination with Lb. casei MnKat were compared first in eukaryotic cell PMA-induced oxidative stress model and then in severe murine DSS-induced colitis. Based on ROS production assays as well as colonic histological scores, a significant reduction of both oxidative stress and inflammation was observed with Lb. casei MnSOD either alone or in combination with Lb. casei MnKat. No added effect of the presence of Lb. casei MnKat was observed. These results suggest that Lb. casei BL23 MnSOD could have anti-inflammatory effects on gut inflammation.
Watterlot
Laurie
L
Unité d'Ecologie et de Physiologie du Système Digestif, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France.
Rochat
Tatiana
T
Sokol
Harry
H
Cherbuy
Claire
C
Bouloufa
Ismael
I
Lefèvre
François
F
Gratadoux
Jean-Jacques
JJ
Honvo-Hueto
Edith
E
Chilmonczyk
Stefan
S
Blugeon
Sébastien
S
Corthier
Gérard
G
Langella
Philippe
P
Bermúdez-Humarán
Luis G
LG
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D002374:Catalase / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use; D002478:Cells, Cultured; D003092:Colitis / Q000139:chemically induced / Q000201:enzymology / Q000382:microbiology* / Q000473:pathology / Q000628:therapy*; D003106:Colon / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000473:pathology; D007780:Lacticaseibacillus casei / Q000201:enzymology* / Q000235:genetics*; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress; D011994:Recombinant Proteins / Q000235:genetics / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use
D000893:Anti-Inflammatory Agents; D011994:Recombinant Proteins; D016264:Dextran Sulfate; D002374:Catalase; D013482:Superoxide Dismutase