title: Cytokine mRNA profile of Epstein-Barr virus-stimulated highly differentiated T cells in multiple sclerosis: a pilot study.
abstract: The reason why EBV-specific cellular immune responses are abnormal in multiple sclerosis (MS) patients is still missing. In this exploratory pilot study, we assessed IL-1beta, IL-2, IL-4, IL-6, IL-10, IL-17, IFN-gamma, TGF-beta1 and FOXP3 mRNA expression in EBV-stimulated highly differentiated T cells (T(HD)) of MS patients and healthy controls (HC). We found increased levels of IFN-gamma and IL-4 mRNA in CD8+ T(HD) cells of MS patients. All the other tested molecules were expressed similarly in MS patients and HC. Interestingly, increased IFN-gamma and IL-4 suggest that the control of EBV replication may be insufficient in MS patients.
authors.affiliation: Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
authors.forename: Emilie
authors.initials: E
authors.lastname: Jaquiéry
authors.forename: Samantha
authors.initials: S
authors.lastname: Jilek
authors.forename: Myriam
authors.initials: M
authors.lastname: Schluep
authors.forename: Géraldine
authors.initials: G
authors.lastname: Le Goff
authors.forename: Miguel
authors.initials: M
authors.lastname: Garcia
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pantaleo
authors.forename: Renaud A
authors.initials: RA
authors.lastname: Du Pasquier
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D020031:Epstein-Barr Virus Infections / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D004854:Herpesvirus 4, Human; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000276:immunology / Q000473:pathology / Q000821:virology; D010865:Pilot Projects; D012333:RNA, Messenger / Q000378:metabolism*; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology
chemical_list: D015703:Antigens, CD; D016207:Cytokines; D012333:RNA, Messenger