title: Chemoselective immobilization of peptides on abiotic and cell surfaces at controlled densities.
abstract: We report herein a new and enabling approach for decorating both abiotic and cell surfaces with the extracellular matrix IKVAV peptide in a site-specific manner using strain promoted azide-alkyne cycloaddition. A cyclooctyne-derivatized IKVAV peptide was synthesized and immobilized on the surface of pancreatic islets through strain-promoted azide-alkyne cycloaddition with cell surface azides generated by the electrostatic adsorption of a cytocompatible poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) copolymer bearing azido groups (PP-N(3)). Both "one-pot" and sequential addition of PP-N(3) and a cyclooctyne-derivatized IKVAV peptide conjugate enabled efficient modification of the pancreatic islet surface in less than 60 min. The ability to bind peptides at controlled surface densities was demonstrated in a quantitative manner using microarrays. Additionally, the technique is remarkably rapid and highly efficient, opening new avenues for the molecular engineering of cellular interfaces and protein and peptide microarrays.
authors.affiliation: Department of Biomedical Engineering, Emory University, Atlanta, Georgia 30322, USA.
authors.forename: Venkata R
authors.initials: VR
authors.lastname: Krishnamurthy
authors.forename: John T
authors.initials: JT
authors.lastname: Wilson
authors.forename: Wanxing
authors.initials: W
authors.lastname: Cui
authors.forename: XueZheng
authors.initials: X
authors.lastname: Song
authors.forename: Yi
authors.initials: Y
authors.lastname: Lasanajak
authors.forename: Richard D
authors.initials: RD
authors.lastname: Cummings
authors.forename: Elliot L
authors.initials: EL
authors.lastname: Chaikof
mesh_terms: D002462:Cell Membrane / Q000737:chemistry; D007515:Islets of Langerhans / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D010455:Peptides
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