Ultrasensitive detection of antibodies using a new Tus-Ter-lock immunoPCR system.
A system consisting of a protein LG coated surface for the capture of mammalian antibodies (target), and an antigen fused to Tus and stoichiometrically linked to a DNA template via the Tus-Ter-lock sequence allowed the ultrasensitive detection of 5.5 attomol of target by real-time immunoPCR in complex media.
Morin
Isabelle
I
Comparative Genomics Centre, School of Pharmacy & Molecular Sciences, DB 21, James Cook Drive, James Cook University, Townsville, QLD 4811, Australia.
Dixon
Nicholas E
NE
Schaeffer
Patrick M
PM
D000906:Antibodies / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D000941:Antigens / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D011485:Protein Binding; D015203:Reproducibility of Results
D000906:Antibodies; D000941:Antigens; C062011:DNA replication terminus site-binding protein, E coli; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C489236:tus protein, E coli; D049452:Green Fluorescent Proteins