Immunopathological studies of Leishmania amazonensis infection in resistant and in susceptible mice.
Leishmania amazonensis infection was studied in mice to evaluate the evolution of leishmaniasis. The association of different methods, such as lesion kinetics, limiting dilution analysis, and immunohistochemistry, established different levels of susceptibility and resistance. Mice were arranged in 3 groups: susceptible (C57BL/10 and CBA), relatively resistant (DBA/2), and resistant (C3H.He). The histopathological analysis of primary lesions and draining lymph nodes showed a predominance of eosinophils and mast cells in the initial phase of infection in all mice. However, the most susceptible mice presented a greater number of amastigotes and higher tissue damage. The immunoglobulin analysis showed that susceptible mice produced high levels of antibodies, whereas resistant and relatively resistant mice exhibited low production of antibodies. Resistant mice showed parasite persistency in the skin and lymph nodes, suggesting that the infection in these mice can be sustained through the infection of cells such as dendritic cells, fibroblasts, and other cells present in these organs.
de Oliveira Cardoso
Flávia
F
Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, Brazil.
de Souza
Celeste da Silva Freitas
Cda S
Mendes
Verônica Gonçalves
VG
Abreu-Silva
Ana Lúcia
AL
Gonçalves da Costa
Sylvio Celso
SC
Calabrese
Kátia da Silva
KS
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004198:Disease Susceptibility; D004804:Eosinophils / Q000276:immunology; D005260:Female; D006651:Histocytochemistry; D007113:Immunity, Innate; D007894:Leishmania mexicana / Q000276:immunology* / Q000472:pathogenicity*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000469:parasitology / Q000473:pathology*; D008198:Lymph Nodes / Q000469:parasitology / Q000473:pathology; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D012867:Skin / Q000469:parasitology / Q000473:pathology
D000913:Antibodies, Protozoan