title: Inhibitory effects of phytoncide solution on melanin biosynthesis.
abstract: To determine the component-activity relationships of phytoncide solutions on inhibitory activity in melanin biosynthesis, four types of phytoncide solution (A-type, AB-type, D-type, and G-type) were evaluated for inhibition of mushroom tyrosinase activity and melanin synthesis on murine B-16 melanoma cells and a human reconstituted skin model. The A-type, AB-type, D-type, and G-type of phytoncide solution treatment resulted in significant inhibition of tyrosinase activity. The amount of melanin was increased by treatment with phytoncide solutions in a concentration-dependent manner on murine B-16 melanoma cells without affecting cell growth. Furthermore, phytoncide solutions also suppressed melanin synthesis in a concentration-dependent manner on a human reconstituted skin model. These effects of A-type solution were superior to those of other solutions.
authors.affiliation: Department of Pharmacy, School of Pharmacy, Kinki University, Higashi-osaka, Osaka, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Fujimori
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Hisama
authors.forename: Hiroharu
authors.initials: H
authors.lastname: Shibayama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kawase
authors.forename: Masahiro
authors.initials: M
authors.lastname: Iwaki
mesh_terms: D000363:Agaricales / Q000201:enzymology; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D008543:Melanins / Q000096:biosynthesis*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D012996:Solutions; D014835:Volatilization
chemical_list: D000975:Antioxidants; D008543:Melanins; D010936:Plant Extracts; D012996:Solutions; D014442:Monophenol Monooxygenase