Development and optimization of a method for the separation of platycosides in Platycodi Radix by comprehensive two-dimensional liquid chromatography with mass spectrometric detection.
Comprehensive two-dimensional chromatography (LCxLC) using combinations of two columns (C(18) x CN and C(18) x NH(2)) was employed with electrospray (ESI) mass spectrometry to analyze platycosides from root extract. Based on the capability of the C(18), CN and NH(2) columns to separate the platycosides, the orthogonality in two-dimensional space according to each combination of columns was predicted from the correlation coefficients between the retention times of the 17 compounds separated by the independent CN and C(18) columns, and NH(2) and C(18) columns. The expected distribution of the peaks was also compared with the two-dimensional plots obtained by practical separation in an LCxLC system. The increased peak capacities using C(18) x NH(2) allowed three minor components and five isomers of the platycosides to be newly separated, which were not identified with 1D-LC using the individual C(18) column, whereas the combination of C(18) x CN did not result in any improvement of the separation performance.
Jeong
Eun-Kyung
EK
Seoul Center, Korea Basic Science Institute, 126-16 Anam-dong, Sungbuk-gu, Seoul 136-713, South Korea.
Cha
Hyun-Jeong
HJ
Ha
Young Wan
YW
Kim
Yeong Shik
YS
Ha
In Jin
IJ
Na
Yun-Cheol
YC
D029422:Campanulaceae / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D006027:Glycosides / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D006027:Glycosides; D010936:Plant Extracts