A porous 3D cell culture micro device for cell migration study.
Cell migration under chemoattractant is an important biological step in cancer metastasis that causes the spread of malignant tumor cells. Porous polymeric materials are widely used to mimic the extracellular matrix (ECM) environment for applications such as three dimensional (3D) cell culturing and tissue engineering. In this paper we report a novel 3D cell culture device based on porous polymeric material to study cancer migration. We fabricated a porous channel on a polymeric chip using a selective ultrasonic foaming method. We demonstrate that a chemical concentration gradient could be established through the porous channel due to the slow diffusion process. We show that significant cell migration could be observed through the porous channel within 1-2 weeks of cell culturing when metastatic M4A4-GFP breast cancer cells were induced by 20% fetal bovine serum (FBS).We also developed a mathematical model to evaluate the diffusivity and concentration gradient through the fabricated porous structure.
Ma
Liang
L
Department of Mechanical Engineering, University of Washington, Seattle, WA 98195-2600, USA.
Zhou
Changchun
C
Lin
Biaoyang
B
Li
Wei
W
D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D054443:Cell Migration Assays / Q000295:instrumentation*; D002465:Cell Movement; D049109:Cell Proliferation; D004058:Diffusion; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry; D016062:Porosity
D011108:Polymers
Biomed Microdevices. 2007 Aug;9(4):597-602
17505887
Lab Chip. 2006 Mar;6(3):381-8
16511621
J Biomed Mater Res A. 2009 Jan;88(1):23-33
18257085
J Cell Sci. 1996 Apr;109 ( Pt 4):793-803
8718671
J Biomater Sci Polym Ed. 2005;16(8):933-47
16128229
Sci Am. 1996 Sep;275(3):72-7
8701296
Methods Mol Biol. 1997;75:449-57
9276291
Cell. 1996 Feb 9;84(3):359-69
8608589
Curr Opin Cell Biol. 2005 Oct;17(5):524-32
16112853
Biomaterials. 2007 Sep;28(26):3815-23
17544499
J Biomed Mater Res A. 2008 Aug;86(2):552-9
18186056
Biomaterials. 2008 Jul;29(20):2962-8
18433863
Exp Cell Res. 2004 Aug 1;298(1):178-87
15242772
Microvasc Res. 2006 Nov;72(3):153-60
17081570
J Exp Med. 1962 Mar 1;115:453-66
13872176
Stem Cells Dev. 2005 Apr;14(2):231-5
15910250
Med Res Rev. 2007 Mar;27(2):149-76
16888756
Methods Mol Biol. 2000;138:113-20
10840748
J Biomed Mater Res B Appl Biomater. 2008 Oct;87(1):121-31
18437710
J Cell Physiol. 2007 Mar;210(3):602-8
17149708
Nat Rev Cancer. 2002 Aug;2(8):563-72
12154349
AAPS PharmSciTech. 2009;10(3):772-9
19499343