title: The development of a mRNA multiplex RT-PCR assay for the definitive identification of body fluids.
abstract: With current methodology, DNA profiling can identify an individual from a sample of biological material but it does not reveal what body fluid or tissue source the DNA profile originated from. We have developed a multiplex PCR system using messenger RNA (mRNA) that can identify blood, saliva, semen and menstrual blood in individual stains or in mixtures of body fluids. Messenger RNA transcripts specific to each type of body fluid have been identified and a multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) system developed to identify these body fluids along with three housekeeping genes. This multiplex can detect semen and seminal fluid (semen without spermatozoa present). Furthermore, we have targeted the co-isolation of RNA and DNA from the same sample and, with the RT-PCR multiplex, we can determine the type of body fluid present as well as generate a DNA profile(s) from the same stain.
authors.affiliation: Institute of Environmental Science and Research Ltd. Mt. Albert Science Centre, Auckland, New Zealand.
authors.forename: Rachel I
authors.initials: RI
authors.lastname: Fleming
authors.forename: SallyAnn
authors.initials: S
authors.lastname: Harbison
mesh_terms: D000818:Animals; D001769:Blood; D002415:Cats / Q000235:genetics; D002645:Chickens / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D016172:DNA Fingerprinting; D004285:Dogs / Q000235:genetics; D004586:Electrophoresis; D005260:Female; D005952:Glucose-6-Phosphatase / Q000235:genetics; D006021:Glycophorins / Q000235:genetics; D006168:Guinea Pigs / Q000235:genetics; D054808:Histatins / Q000235:genetics; D006801:Humans; D008297:Male; D053507:Matrix Metalloproteinase 11 / Q000235:genetics; D051379:Mice / Q000235:genetics; D011479:Protamines / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification*; D011817:Rabbits / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012463:Saliva; D012471:Salivary Proteins and Peptides / Q000235:genetics; D012661:Semen; D013552:Swine / Q000235:genetics; D035602:Transcriptional Elongation Factors / Q000235:genetics; D011503:Transglutaminases / Q000235:genetics; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics
chemical_list: D006021:Glycophorins; C068075:HTN3 protein, human; D054808:Histatins; D011479:Protamines; D012333:RNA, Messenger; C018149:STATH protein, human; D012471:Salivary Proteins and Peptides; D035602:Transcriptional Elongation Factors; C068621:protamine 2; C018648:transcription factor S-II; D004247:DNA; C418702:transglutaminase 4; D011503:Transglutaminases; D044763:Ubiquitin-Conjugating Enzymes; D005952:Glucose-6-Phosphatase; D053507:Matrix Metalloproteinase 11