[Separation of derivatized aliphatic aldehydes in beverage by nonaqueous capillary electrophoresis].
A simple and mild method for the separation of aliphatic aldehydes based on a condensation reaction with 9,10-phenanthrenequinone as labeling reagent with nonaqueous capillary electrophoresis has been developed. The detection was performed with a diode array detector (DAD). A 58.5 cm (50 cm effective length) x 50 microm i.d. untreated fused-silica capillary was used. To optimize the conditions, the background electrolyte concentration, column temperature, voltage and other factors were evaluated. The results indicated that the buffer concentration had a great impact on the separation, but the influences of temperature and added additives on the resolution were not obvious. The optimized conditions were as follows: 80 mmol/L ammonium acetate, 1.4 mol/L acetic acid, voltage of 28 kV, column temperature of 20 degrees C and DAD detection at 254 nm. The samples were introduced atmospherically with the injection at 5 kPa (50 mbar) for 8 s. The results indicate that seven aliphatic aldehyde derivatives and actual samples can be achieved baseline resolution under the proposed conditions.
Bai
Xinwei
X
Key Laboratory of Life-Organic Analysis of Shandong Province, College of Chemistry Science, Qufu Normal University, Qufu 273165, China.
Wang
Yanbao
Y
Zhao
Huaixin
H
Sun
Zhiwei
Z
Xia
Lian
L
Fu
Yanyan
Y
Suo
Yourui
Y
Li
Yulin
Y
You
Jinmao
J
D000447:Aldehydes / Q000302:isolation & purification*; D001628:Beverages / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*
D000447:Aldehydes