title: A Microfluidic Platform for Characterization of Protein-Protein Interactions.
abstract: Traditionally, expensive and time consuming techniques such as mass spectrometry and Western Blotting have been used for characterization of protein-protein interactions. In this paper, we describe the design, fabrication, and testing of a rapid and inexpensive sensor, involving the use of microelectrodes in a microchannel, which can be used for real-time electrical detection of specific interactions between proteins. We have successfully demonstrated detection of target glycoprotein-glycoprotein interactions, antigen-antibody interactions, and glycoprotein-antigen interactions. We have also demonstrated the ability of this technique to distinguish between strong and weak interactions. Using this approach, it may be possible to multiplex an array of these sensors onto a chip and probe a complex mixture for various types of interactions involving protein molecules.
authors.affiliation: M. Javanmard and F. Pease are with the Department of Electrical Engineering, Stanford University, Stanford, CA 94305 USA ( mehdij@stanford.edu ; pease@cis.stanford.edu ). A. H. Talasaz, M. Nemat-Gorgani, D. E. Huber, and R. W. Davis are with the Stanford Genome Technology Center, Palo Alto, CA 94304 USA ( atalasaz@stanford.edu ; mohsenn@stanford.edu ; david.huber@stanford.edu ; dbowe@stanford.edu ). M. Ronaghi is with Illumina Inc., San Diego, CA 92121 USA ( mronaghi@illumina.com ).
authors.forename: Mehdi
authors.initials: M
authors.lastname: Javanmard
authors.forename: Amirali H
authors.initials: AH
authors.lastname: Talasaz
authors.forename: Mohsen
authors.initials: M
authors.lastname: Nemat-Gorgani
authors.forename: David E
authors.initials: DE
authors.lastname: Huber
authors.forename: Fabian
authors.initials: F
authors.lastname: Pease
authors.forename: Mostafa
authors.initials: M
authors.lastname: Ronaghi
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Davis
references.citation: Nat Genet. 2002 Dec;32 Suppl:526-32
references.pmid: 12454649
references.citation: J Colloid Interface Sci. 2006 Jan 15;293(2):500-4
references.pmid: 16102778
references.citation: Lab Chip. 2009 May 21;9(10):1429-34
references.pmid: 19417910
references.citation: Electrophoresis. 2000 Jan;21(1):27-40
references.pmid: 10634468
references.citation: Biosens Bioelectron. 2005 Jan 15;20(7):1320-6
references.pmid: 15590285
references.citation: Biosens Bioelectron. 2004 May 15;19(10):1139-47
references.pmid: 15046744
references.citation: J Colloid Interface Sci. 2007 Jun 1;310(1):74-82
references.pmid: 17346726
references.citation: Anal Biochem. 1981 Apr;112(2):195-203
references.pmid: 6266278
references.citation: Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):820-4
references.pmid: 12552089
references.citation: Biomicrofluidics. 2007 Nov 30;1(4):44103
references.pmid: 19693402
references.citation: Lab Chip. 2005 Oct;5(10):1155-60
references.pmid: 16175273
references.citation: Lab Chip. 2007 May;7(5):603-10
references.pmid: 17476379
references.citation: Biomicrofluidics. 2007 May 10;1(2):21503
references.pmid: 19693376
references.citation: Lab Chip. 2001 Sep;1(1):76-82
references.pmid: 15100895
references.citation: Biosens Bioelectron. 2003 Dec 30;19(5):495-502
references.pmid: 14623474
references.citation: J Colloid Interface Sci. 2002 Feb 15;246(2):223-6
references.pmid: 16290405