Development of efficient tools for genetic manipulation of dermatophytes.
Molecular biological approaches have recently begun to be applied to molecular genetics studies of dermatophytes. High-throughput gene analysis methodologies, such as EST sequencing, differential cDNA screening, and cDNA-based microarray analysis have been used to obtain information on many dermatophyte genes and their expression profiles under different experimental conditions. In addition, whole genome sequencing projects are underway for several important dermatophytes, such as Trichophyton rubrum and Microsporum canis. These studies will provide large amounts of valuable information for elucidating the molecular basis of host invasion by dermatophytes and their virulence. Targeted gene disruption by homologous recombination is one of the most common approaches for determining the functions and roles of numerous genes isolated from pathogenic fungi. However, the difficulty of genetic manipulation due to low transformation frequency of dermatophytes may limit the successful production of null mutants by targeted gene disruption via homologous recombination. To overcome these problems, our group has developed useful genetic manipulation systems for dermatophytes using the clinically important dermatophyte, T. mentagrophytes.
Yamada
Tsuyoshi
T
Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan.
D003883:Arthrodermataceae / Q000235:genetics* / Q000472:pathogenicity*; D018390:Gene Targeting / Q000379:methods; D005818:Genetic Engineering / Q000379:methods*; D005821:Genetic Techniques; D014170:Transformation, Genetic