The static magnetic field accelerates the osteogenic differentiation and mineralization of dental pulp cells.
Dental pulp cells (DPCs) can differentiate into osteoblasts and are deemed a promising cell source for bone regeneration. Static magnetic field (SMF) stimulates osteoblast differentiation but the effect in DPCs remains unknown. The aim of this study was to investigate the effect of SMF exposure on the osteogenic differentiation and mineralization of rat DPCs in vitro. Cells were continuously exposed to SMF at 290 mT in the presence/absence of osteogenic induction [dexamethasone (Dex)/beta-glycerophosphate (beta-GP)]. Results showed that SMF alone did not impair the cell cycle and proliferation. On the other hand, obvious condensation in the metachromatic staining of the extracellular matrix with toluidine blue was observed for SMF-exposed cells as well as the Dex/beta-GP treated cells. SMF in combination with Dex/beta-GP significantly increased the mRNA expression of osteogenic genes, as well as the ALP activity and extracellular calcium concentration at the early stage, followed by obvious calcium deposits later. Besides, SMF exposure increased the activity of extracellular signal-regulated kinase 1/2 (ERK1/2) at 3 h and accelerated the mRNA expression of osteogenic transcription factor, Cbfa1, advancing its activation time from 168 to 72 h under osteogenic induction. In summary, SMF exposure in combination of Dex/beta-GP induction could significantly accelerate the osteogenic differentiation and mineralization of DPCs.
Hsu
Shan-Hui
SH
Institute of Polymer Science and Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 10617, Taipei, Taiwan, Republic of China, shhsu@ntu.edu.tw.
Chang
Jui-Chih
JC
J Dent Res. 2002 Aug;81(8):531-5
12147742
Prog Biophys Mol Biol. 2005 Feb-Apr;87(2-3):213-23
15556660
Bioelectromagnetics. 2006 Jan;27(1):35-42
16283651
J Toxicol Environ Health A. 1998 May 8;54(1):63-76
9588349
Connect Tissue Res. 2007;48(5):229-38
17882698
J Cell Physiol. 2006 Aug;208(2):319-25
16622855
Int J Adult Orthodon Orthognath Surg. 1997;12(1):43-53
9456617
Bioelectromagnetics. 1999;20(3):177-82
10194560
Mol Endocrinol. 1997 Jul;11(8):1103-13
9212058
Exp Cell Res. 2007 Jan 1;313(1):22-37
17081517
Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13625-30
11087820
J Cell Sci. 1993 Jun;105 ( Pt 2):317-31
8408268
Biochem Biophys Res Commun. 2003 Nov 7;311(1):32-8
14575691
Bone. 2000 Aug;27(2):227-31
10913915
J Bone Miner Res. 2002 Oct;17(10):1814-21
12369785
J Dent Res. 2003 Dec;82(12):976-81
14630898
J Biol Chem. 1998 Nov 13;273(46):30509-16
9804819
Phys Med Biol. 2007 Feb 21;52(4):889-98
17264359
Stem Cell Rev. 2008 Spring;4(1):21-6
18300003
J Tissue Eng Regen Med. 2007 Jan-Feb;1(1):66-73
18038394
Br J Orthod. 1999 Mar;26(1):29-37
10333885
Arch Oral Biol. 1967 May;12(5):639-44
4225693
J Bone Miner Res. 2005 Aug;20(8):1394-402
16007337
J Craniofac Surg. 2008 Jan;19(1):204-10
18216690
J Cell Biochem. 1993 Apr;51(4):387-93
8496242
Bioelectrochemistry. 2008 Feb;72(1):41-6
18160349
Tissue Eng. 2005 Mar-Apr;11(3-4):357-68
15869416
Arch Oral Biol. 1993 Sep;38(9):769-77
8240085
Cell Tissue Res. 2005 Sep;321(3):391-400
15988617
Cells Tissues Organs. 2004;176(1-3):109-19
14745240
Biochem J. 1984 May 1;219(3):1057-9
6743242
Am J Orthod Dentofacial Orthop. 1995 Jun;107(6):578-88
7771362
J Biol Chem. 2000 Feb 11;275(6):4453-9
10660618
Am J Orthod Dentofacial Orthop. 1993 Mar;103(3):240-6
8456781
Endocrinology. 2003 Feb;144(2):412-22
12538600
Electromagn Biol Med. 2008;27(1):55-64
18327714
J Biol Chem. 1998 Dec 4;273(49):32988-94
9830051
Sci STKE. 2004 Aug 31;2004(249):RE12
15353762
Med Eng Phys. 1998 Sep;20(6):397-402
9796945
Calcif Tissue Int. 2000 Feb;66(2):139-44
10652962
Arch Oral Biol. 1971 Oct;16(10):1143-54
4109159
J Dent Res. 2003 Dec;82(12):962-6
14630895
J Histochem Cytochem. 2002 Jul;50(7):911-21
12070270
Development. 1999 Jul;126(13):2911-20
10357935
Radiat Res. 2000 May;153(5 Pt 2):706-14
10790296
Exp Cell Res. 1995 May;218(1):255-62
7737363
J Endod. 2001 Mar;27(3):180-2
11487147
Int J Dev Biol. 1995 Dec;39(6):881-93
8901191
Eur Cell Mater. 2009 Nov 12;18:75-83
19908196
J Cell Biochem. 2007 Aug 1;101(5):1266-77
17265428
J Cell Physiol. 2008 Jan;214(1):166-72
17565721