Evaluation of cytotoxic activity of Schisandra chinensis lignans.
Using exhaustive chromatographic separation we have isolated (-)-tigloyl-deangeloyl-gomisin F as a novel dibenzocyclooctadiene lignan from schisandra chinensis. With the help of HPLC, we further isolated (+)-schisandrin, (+)-deoxyschisandrin, (+)-γ-schisandrin, (-)-gomisin J, (+)-gomisin A, (-)-gomisin N, (-)-tigloyl-gomisin P, (-)-wuweizisu C, (-)-gomisin D, rubrisandrin A, (-)-gomisin G, (+)-gomisin K (3) and (-)-schisantherin C. A full NMR description of (-)-schisantherin C was carried out with the aim to confirm previous reports of its structure. Compounds isolated were identified on the basis of UV, IR, (1)H- and (13)C-NMR and MS. The cytotoxicity of lignans was tested for the BY-2 cell line alone and as a synergistic effect with the cytotoxic agent camptothecin. Lignans showed various toxicity and synergistic and antagonistic effects on camptothecin-induced cytotoxicity. Cytotoxicity against colon cancer cell line LoVo was also tested.
Smejkal
Karel
K
Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. karel.mejkal@post.cz
Slapetová
Tereza
T
Krmenčík
Pavel
P
Babula
Petr
P
Dall'Acqua
Stefano
S
Innocenti
Gabbriella
G
Vančo
Ján
J
Casarin
Elisabetta
E
Carrara
Maria
M
Kalvarová
Karolína
K
Dvorská
Margita
M
Slanina
Jiří
J
Kramářová
Eva
E
Julínek
Ondřej
O
Urbanová
Marie
M
D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry; D032141:Schisandra / Q000737:chemistry*; D014026:Nicotiana / Q000187:drug effects
D003603:Cytotoxins; D017705:Lignans; D010936:Plant Extracts