Avian metapneumovirus (AMPV) attachment protein involvement in probable virus evolution concurrent with mass live vaccine introduction.
Avian metapneumoviruses detected in Northern Italy between 1987 and 2007 were sequenced in their fusion (F) and attachment (G) genes together with the same genes from isolates collected throughout western European prior to 1994. Fusion protein genes sequences were highly conserved while G protein sequences showed much greater heterogeneity. Phylogenetic studies based on both genes clearly showed that later Italian viruses were significantly different to all earlier virus detections, including early detections from Italy. Furthermore a serine residue in the G proteins and lysine residue in the fusion protein were exclusive to Italian viruses, indicating that later viruses probably arose within the country and the notion that these later viruses evolved from earlier Italian progenitors cannot be discounted. Biocomputing analysis applied to F and G proteins of later Italian viruses predicted that only G contained altered T cell epitopes. It appears likely that Italian field viruses evolved in response to selection pressure from vaccine induced immunity.
Cecchinato
Mattia
M
Dipartimento di Sanità Pubblica, Patologia Comparata e Igiene Veterinaria, Faculty of Veterinary Medicine, University of Padua, Viale dell'Università 16, 35020 Legnaro, PD, Italy. mattia.cecchinato@unipd.it
Catelli
Elena
E
Lupini
Caterina
C
Ricchizzi
Enrico
E
Clubbe
Jayne
J
Battilani
Mara
M
Naylor
Clive J
CJ
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D005060:Europe; D019204:GTP-Binding Proteins / Q000235:genetics; D007558:Italy; D029121:Metapneumovirus / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D018184:Paramyxoviridae Infections / Q000517:prevention & control / Q000821:virology; D010802:Phylogeny; D011201:Poultry Diseases / Q000517:prevention & control / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014422:Turkeys / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics*; D014765:Viral Vaccines / Q000494:pharmacology*
D014759:Viral Envelope Proteins; D014765:Viral Vaccines; D019204:GTP-Binding Proteins