[Comparison of two nonalcoholic hepatocellular steatosis models].
OBJECTIVE
To compare two models of nonalcoholic hepatocellular steatosis.


METHODS
HL-7702 cells were incubated with a mixture of of unsaturated oleate acid or 50% fetal bovine serum to induce fat-overloading. Significant fat accumulation was documented by Oil Red O staining , and intracellular triglyceride levels was detected by triglyceride enzymatic assay.


RESULTS
The results showed that both 0.5 mmol/ml oleate acid and 50% FBS were able to induce nonalcoholic hepatocellular steatosis.


CONCLUSIONS
A nonalcoholic hepatocellular steatosis was induced by 0.5 mmol/ml oleate acid.
Wu
Hong-ru
HR
Department of Gastroenterology, the First Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou, China.
Chen
Shao-hua
SH
Lu
Yi
Y
Li
You-ming
YM
D002460:Cell Line; D005234:Fatty Liver; D022781:Hepatocytes / Q000473:pathology*; D006801:Humans; D008954:Models, Biological