title: [Induction of IL-8 by Chlamydia trachomatis through MAPK pathway rather than NF-kappaB pathway].
abstract: OBJECTIVE
  To determine the signaling pathway required for Chlamydial induction of IL-8 expression in epithelial cells.
  
  
  METHODS
  The production and localization of IL-8 in Chlamydia-infected Hela 229 cells were monitored using Western blot, immunoflourescence, and ELISA. Activation of MAPK and NF-kappaB signaling pathways were detected by Western blot and immunoflourescence. The effect of different signaling pathways on Chlamydia-induced Il-8 was measured by experiments of chemical inhibitors.
  
  
  RESULTS
  IL-8 was induced by Chlamydia and was time-dependant. Chlamydial infection activated MAPK/ERK and MAPK/p38 pathways but not NF-kappaB pathway. Chlamydial induction of IL-8 was blocked by small molecule inhibitors targeting the ERK and p38 pathways.
  
  
  CONCLUSIONS
  Chlamydia-induced IL-8 in cervical epithelial cells, the natural target cell type of Chlamydia trachomatis infection, is dependent on MAPK pathway but not NF-kappaB pathway, which provides important information for further understanding the molecular mechanism of Chlamydia-induced inflammatory pathologies.
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Cheng
authors.forename: Saidan
authors.initials: S
authors.lastname: Zhang
authors.forename: Guangming
authors.initials: G
authors.lastname: Zhong
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
mesh_terms: D002690:Chlamydia Infections / Q000378:metabolism; D002692:Chlamydia trachomatis / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology; D006367:HeLa Cells; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8; D016328:NF-kappa B; D048051:p38 Mitogen-Activated Protein Kinases