A simple and fast method to analyze the orientation of c-type cytochromes in the outer membrane of Gram-negative bacteria.
Outer membrane cytochromes catalyze the final reduction step of respiratory chains to electron acceptors that cannot diffuse through the outer membrane of Gram-negative bacteria. We developed an in vivo method to detect the orientation of outer membrane cytochromes via analysis of electron transfer reactions between these enzymes and riboflavin.
Richter
Katrin
K
Department of Microbiology, University of Freiburg, D-79104 Freiburg, Germany.
Bücking
Clemens
C
Schicklberger
Marcus
M
Gescher
Johannes
J
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D045304:Cytochromes c / Q000032:analysis*; D004579:Electron Transport; D006090:Gram-Negative Bacteria / Q000201:enzymology*; D010084:Oxidation-Reduction; D012256:Riboflavin / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D045304:Cytochromes c; D012256:Riboflavin