[Research the role of gulonic acid lactone in the five kinds of organic phosphorus pesticide detection].
OBJECTIVE
To evaluate the protective effect of gulonic acid lactone in the gas chromatography-mass spectrometry system for detecting phorate, diazinon, dimethoate, methyl parathion, malathion five kinds of organic phosphorus pesticide.


METHODS
By comparing the peak area changes of organic phosphorus pesticide configured with different concentrations (0, 0.3, 0.6, 0.9 and 1.2 mg/ml) of the gulonic acid lactone-methanol solution, and the concentrations (500 ng/ml) of organic phosphorus pesticide are same, to determine the best dosage of gulonic acid lactone. In the concentration, evaluate the protective effect of gulonic acid lactone on five kinds of organic phosphorus pesticide testing.


RESULTS
When the concentration of gulonic acid lactone-methanol solution <1 mg/ml, the selected ion peak area of the five kinds organophosphorus pesticides detected by gas chromatography-mass spectrometry in the same conditions increases with the upward trend of its concentration ; when the concentration is equal to 1 mg/ml, the peak area reached the highest level, and then with the concentrations increased, no significant change in it. Diazinon's sensitivity increased by 4 times, phorate's sensitivity increased by 5 times, dimethoate, methyl parathion and malathion's sensitivity increased by 10 times, and the peak shape improved distinctly, tailing phenomenon disappears.


CONCLUSIONS
Gulonic acid lactone can effectively improve the five kinds of organic phosphorus pesticide's sensitivity, improving the peak-type tailing and asymmetric phenomenon by the result of the active site. Quantitative bias can be effectively corrected caused by matrix effects. When the concentration is 1 mg/ml, it has the best effect of the compensation effect.
Pan
Xingfu
X
Shandong Academy of Occupational Health and Occupational Medicine, Ji' nan 250062, China.
Du
Huifang
H
Yan
Huifang
H
Shao
Hua
H
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005942:Gluconates; D007783:Lactones; D008294:Malathion / Q000032:analysis; D008743:Methyl Parathion / Q000032:analysis; D009943:Organophosphorus Compounds / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D010702:Phorate / Q000032:analysis
D005942:Gluconates; D007783:Lactones; D009943:Organophosphorus Compounds; D010573:Pesticide Residues; D010702:Phorate; D008743:Methyl Parathion; D008294:Malathion; C010730:beta-glucono-1,5-lactone