Chemical characteristics and hemostatic  performances of ordered mesoporous  calcium-doped silica xerogels.
A well-ordered mesoporous silicon dioxide calcium-doped xerogel (m-SXC) was synthesized by a sol–gel process. The in vitro bioactivity of the m-SXC was investigated by analyzing the apatite-forming ability in simulated body fluid (SBF) and MC3T3-E1 cell culture with materials, and the hemostatic activity of the m-SXC was determined by measuring the activated partial thromboplastin time (APTT) and prothrombin time (PT) in vitro. The results suggested that the m-SXC exhibited excellent in vitro bioactivity, with surface apatite formation for them-SXC containing 10 w% Ca (m-SXC10) exceeding that containing 5 w% Ca (n-SXC5) and 0% Ca (m-SXC0) at 7 days, and the cell response test (MTT assay) and cell morphology observation demonstrated that these materials were biocompatible with no obvious negative effects on cellular viability. Moreover, the m-SXC with a high surface area (m-SXC0: 489.9 m(2) g(-1), m-SXC5: 466.5 m2 g(-1), m-SXC10: 438.1 m(2) g(-1)) possessed good hemostatic property because it could absorb a large amount of water from the blood, which could concentrate the components of blood and reduce the clotting time. Furthermore, the addition of calcium in the m-SXC had significant effects on the clotting time, in which the APTT(m-SXC0: 8 s, m-SXC5: 7 s, m-SXC10: 6 s, respectively) and PT (m-SXC0: 16 s, m-SXC5:11 s, m-SXC10: 10 s, respectively) were shortened with the increase of Ca amounts in them-SXC, indicating that incorporation of Ca in the m-SXC could improve its hemostatic activity.
Wu
Xiaohui
X
Key Laboratory for Ultrafine Materials of Ministry of Education, and Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People’s Republic of China.
Wei
Jie
J
Lu
Xun
X
Lv
Yang
Y
Chen
Fangping
F
Zhang
Yanglan
Y
Liu
Changsheng
C
D016475:3T3 Cells; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects / Q000502:physiology*; D001826:Body Fluids / Q000737:chemistry; D002118:Calcium / Q000008:administration & dosage / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects*; D006490:Hemostatics / Q000008:administration & dosage* / Q000138:chemical synthesis*; D020100:Hydrogels / Q000008:administration & dosage / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D016062:Porosity; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000737:chemistry*
D006490:Hemostatics; D020100:Hydrogels; D012822:Silicon Dioxide; D002118:Calcium