A small nucleolar RNA functions in rRNA processing in Caenorhabditis elegans.
CeR-2 RNA is one of the newly identified Caenorhabditis elegans noncoding RNAs (ncRNAs). The characterization of CeR-2 by RNomic studies has failed to classify it into any known ncRNA family. In this study, we examined the spatiotemporal expression patterns of CeR-2 to gain insight into its function. CeR-2 is expressed in most cells from the early embryo to adult stages. The subcellular localization of this RNA is analogous to that of fibrillarin, a major protein of the nucleolus. It was observed that knockdown of C/D small nucleolar ribonucleoproteins (snoRNPs), but not of H/ACA snoRNPs, resulted in the aberrant nucleolar localization of CeR-2 RNA. A mutant worm with a reduced amount of cellular CeR-2 RNA showed changes in its pre-rRNA processing pattern compared with that of the wild-type strain N2. These results suggest that CeR-2 RNA is a C/D snoRNA involved in the processing of rRNAs.
Hokii
Yusuke
Y
Functional Genomics and Technology, United Graduate School of Agricultural Science, Iwate University, 18-8 Ueda 3-chome, Morioka 020-8550.
Sasano
Yumi
Y
Sato
Mayu
M
Sakamoto
Hiroshi
H
Sakata
Kazumi
K
Shingai
Ryuzo
R
Taneda
Akito
A
Oka
Shigenori
S
Himeno
Hyouta
H
Muto
Akira
A
Fujiwara
Toshinobu
T
Ushida
Chisato
C
D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012322:RNA Precursors / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000378:metabolism*; D020537:RNA, Small Nucleolar / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D020718:Ribonucleoproteins, Small Nucleolar / Q000235:genetics; D016415:Sequence Alignment
D012322:RNA Precursors; D012335:RNA, Ribosomal; D020537:RNA, Small Nucleolar; D020718:Ribonucleoproteins, Small Nucleolar
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