title: [Relationship between genetic polymorphisms of DAT1 VNTR and cancer with abnormal Hilit].
abstract: OBJECTIVE
  This study was to investigate the relationship between the 40 bp VNTR polymorphism of dopamine transporter gene (DAT1) and cancer with Uighur medicine abnormal Hilit on Chinese Uighur population of Xinjiang province.
  
  
  METHODS
  Divided the cancer patients into four body fluids according to Uighur medicine theory, the polymerase chain reaction and VNTR polymorphism technique was employed to detect genotype and allele frequencies of a 40 bp VNTR polymorphism situated in 3' untranslated region of the DAT1 gene in 47 cancer patients with abnormal black Savda, 26 cancer patients with other abnormal Hilit and 57 normal control subjects in Uighur population of Xinjiang Province.
  
  
  RESULTS
  (1) In our sample,the repeat numbers of 40 bp were 6 to 11 (PCR product length of 320 bp to 520 bp) and 10-repeats allele (480 bp) detected was the dominant allele of DAT1 gene polymorphisms with the allele frequency 90.4%. Six kinds of genotype were detected in this study and the genotype 480 bp/480 bp was the most common genotype with genotype frequency 80.7%. (2) The susceptibility to cancer patients with abnormal black Savda among the subjects with 10 repeat (R) allele and 10/10 genotypes and the subjects with non-10 repeat (R) allele and non-10/10 genotypes were similar (P = 0.158, OR = 1.994, 95% CI = 0.754-5.275; P = 0.138, OR = 2.143, 95% CI = 0.772-5.947). No significant differences for the genotype frequency or the allele frequency of the 40 bp VNTR polymorphism of DAT1 were revealed between cancer patients with abnormal black Savda and cancer patients with other abnormal Hilit (P = 0.729, P = 0.782).
  
  
  CONCLUSIONS
  The 40 bp VNTR polymorphism of DAT1 may not be correlated to the susceptibility to cancer with Uighur medicine abnormal Hilit.
authors.affiliation: College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. woshipengxiaomei@163.com
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Peng
authors.forename: Hamulati
authors.initials: H
authors.lastname: Wupuer
authors.forename: Dolkun
authors.initials: D
authors.lastname: Maitiyusup
authors.forename: Palida
authors.initials: P
authors.lastname: Kurban
authors.forename: Wencheng
authors.initials: W
authors.lastname: Dai
authors.forename: Tursun
authors.initials: T
authors.lastname: Mamat
mesh_terms: D000328:Adult; D000368:Aged; D002681:China; D050483:Dopamine Plasma Membrane Transport Proteins / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008519:Medicine, Traditional; D008875:Middle Aged; D018598:Minisatellite Repeats; D009369:Neoplasms / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; C493214:SLC6A3 protein, human