title: MicroRNA expression in the embryonic mouse inner ear.
abstract: Although microRNA (miRNA) is expressed extensively in the postnatal mouse inner ear, its expression in the sensory epithelium during embryogenesis has not been well characterized. We investigated miRNA expression at E13.5 and E16.5 by microarray analysis, quantitative real-time-PCR, and in-situ hybridization. MiRNA-182, miRNA-140, miRNA-200c, and others showed distinct temporal and spatial expression patterns. MiRNA-194, whose expression in zebrafish seems to play an important role in the differentiation of the intestinal epithelium, was also expressed in the spiral ganglia of the mouse inner ear, where it may play a similar role in neuronal differentiation. Our results indicate that miRNAs are widely expressed in the developing inner ear, with more species recruited as hair cells differentiate, suggesting an important developmental role.
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, PR China.
authors.forename: Xian-Ren
authors.initials: XR
authors.lastname: Wang
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhen
authors.forename: Pen-Xing
authors.initials: PX
authors.lastname: Zhang
authors.forename: Geng
authors.initials: G
authors.lastname: Xu
authors.forename: Hongyan
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000465:Algorithms; D000818:Animals; D007758:Ear, Inner / Q000196:embryology* / Q000378:metabolism*; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000378:metabolism*; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012984:Software; D013136:Spiral Ganglion / Q000196:embryology / Q000378:metabolism
chemical_list: C547828:MIRN140 microRNA, mouse; D035683:MicroRNAs; C540524:Mirn182 microRNA, mouse