title: Immunohistochemical labeling of the inhibin/activin betaC subunit in normal human placental tissue and chorionic carcinoma cell lines.
abstract: Inhibins and activins are important regulators of the female reproductive system. A novel inhibin subunit, named betaC, has been identified and demonstrated to be expressed in several human tissues. We demonstrate here that inhibin betaC is expressed in human placenta. Expression of the inhibin betaC subunit was demonstrated at the protein level by means of immunohistochemical evaluation and at the transcriptional level by an inhibin betaC-specific RT-PCR analysis. Expression of inhibin betaC was detected in the human chorionic carcinoma cell lines JEG and BeWo. Although the precise role of this novel inhibin subunit in human placenta development and homeostasis is unclear, analogies with other inhibin subunits and the strong expression of betaC in normal human trophoblast cells and chorionic carcinoma cells suggest that betaC may be involved in autocrine/paracrine signaling pathways, angiogenesis, decidualization, and tissue remodeling under normal and malignant conditions. Additionally, JEG and BeWo express betaC and, therefore, can be used as a cell culture model for further functional analysis of this subunit in the human placenta.
authors.affiliation: First Department of Obstetrics and Gynecology, Ludwig-Maximilians-University Munich, Munich, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Weissenbacher
authors.forename: Ansgar
authors.initials: A
authors.lastname: Brüning
authors.forename: Tanja
authors.initials: T
authors.lastname: Kimmich
authors.forename: Josef
authors.initials: J
authors.lastname: Makovitzky
authors.forename: Andrea
authors.initials: A
authors.lastname: Gingelmaier
authors.forename: Ioannis
authors.initials: I
authors.lastname: Mylonas
mesh_terms: D002277:Carcinoma / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D028322:Inhibin-beta Subunits / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism* / Q000648:ultrastructure; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D028322:Inhibin-beta Subunits
references.citation: Cytokine Growth Factor Rev. 2005 Aug-Oct;16(4-5):377-85
references.pmid: 15925536
references.citation: Placenta. 2000 Mar-Apr;21 Suppl A:S113-9
references.pmid: 10831135
references.citation: J Reprod Dev. 2006 Aug;52(4):487-95
references.pmid: 16627954
references.citation: Endocr Rev. 2002 Dec;23(6):787-823
references.pmid: 12466190
references.citation: Mol Cell Endocrinol. 2001 Jun 30;180(1-2):123-30
references.pmid: 11451581
references.citation: Hum Reprod. 2007 May;22(5):1304-9
references.pmid: 17307808
references.citation: Am J Pathol. 2009 Jan;174(1):184-95
references.pmid: 19095948
references.citation: J Mol Histol. 2008 Oct;39(5):535-41
references.pmid: 18781389
references.citation: Histochem Cell Biol. 2004 Nov;122(5):461-71
references.pmid: 15480736
references.citation: Endocrinology. 1997 Sep;138(9):3976-86
references.pmid: 9275089
references.citation: Exp Biol Med (Maywood). 2002 Feb;227(2):75-87
references.pmid: 11815670
references.citation: N Engl J Med. 1996 May 9;334(19):1231-6
references.pmid: 8606718
references.citation: Cell Commun Signal. 2009 Jun 18;7:15
references.pmid: 19538713
references.citation: Biochem Biophys Res Commun. 1996 Nov 21;228(3):669-74
references.pmid: 8941337
references.citation: Placenta. 2000 Jan;21(1):38-43
references.pmid: 10692249
references.citation: Mol Hum Reprod. 2003 Apr;9(4):199-203
references.pmid: 12651901
references.citation: J Clin Endocrinol Metab. 2000 Dec;85(12):4851-8
references.pmid: 11134153
references.citation: Placenta. 2007 Feb-Mar;28(2-3):107-17
references.pmid: 16712928
references.citation: Reprod Biol Endocrinol. 2009 Jul 14;7:70
references.pmid: 19602262
references.citation: Eur J Cancer. 2009 May;45(7):1304-1314
references.pmid: 19231155
references.citation: Mol Cell Endocrinol. 2004 Jul 30;222(1-2):61-9
references.pmid: 15249126
references.citation: Genomics. 1996 Mar 15;32(3):358-66
references.pmid: 8838799
references.citation: Aust N Z J Obstet Gynaecol. 2006 Jun;46(3):245-9
references.pmid: 16704482
references.citation: Endocr J. 2005 Apr;52(2):169-75
references.pmid: 15863943
references.citation: Am J Obstet Gynecol. 2000 Mar;182(3):737-9
references.pmid: 10739543
references.citation: Mol Hum Reprod. 2000 Aug;6(8):743-9
references.pmid: 10908285
references.citation: Exp Biol Med (Maywood). 2002 Oct;227(9):724-52
references.pmid: 12324653
references.citation: Mol Cell Endocrinol. 2004 Dec 30;228(1-2):103-19
references.pmid: 15541575
references.citation: J Mol Histol. 2006 Jan;37(1-2):43-52
references.pmid: 16670820
references.citation: Histopathology. 1998 Mar;32(3):252-6
references.pmid: 9568511
references.citation: Lancet. 1997 May 3;349(9061):1285-8
references.pmid: 9142063
references.citation: Am J Obstet Gynecol. 1991 Sep;165(3):750-8
references.pmid: 1892206
references.citation: Anticancer Res. 2007 Jul-Aug;27(4A):1995-2000
references.pmid: 17649811
references.citation: Recent Prog Horm Res. 1988;44:1-34
references.pmid: 3064204
references.citation: Zentralbl Gynakol. 2004 Jun;126(3):148-53
references.pmid: 15236099
references.citation: Carcinogenesis. 2003 Nov;24(11):1801-9
references.pmid: 12949049
references.citation: Endocr Pathol. 2006 Spring;17(1):19-33
references.pmid: 16760577
references.citation: Mol Cell Biol. 2000 Aug;20(16):6127-37
references.pmid: 10913194
references.citation: Biochem Biophys Res Commun. 1997 Jun 27;235(3):553-6
references.pmid: 9207194
references.citation: Oncol Rep. 2007 Jan;17(1):97-104
references.pmid: 17143484
references.citation: Arch Gynecol Obstet. 2010 Aug;282(2):185-91
references.pmid: 20012305
references.citation: Endocrinology. 2003 Aug;144(8):3497-504
references.pmid: 12865331
references.citation: Histochem Cell Biol. 2009 Jul;132(1):33-8
references.pmid: 19363681
references.citation: J Mol Endocrinol. 1996 Aug;17(1):61-5
references.pmid: 8863188
references.citation: J Mol Endocrinol. 2005 Apr;34(2):505-15
references.pmid: 15821113
references.citation: Endocr J. 1997 Oct;44(5):759-64
references.pmid: 9466335
references.citation: Endocrinology. 2003 Oct;144(10):4410-9
references.pmid: 12960042
references.citation: Biochem Biophys Res Commun. 1995 Jan 17;206(2):608-13
references.pmid: 7826378
references.citation: Biol Reprod. 2000 May;62(5):1224-30
references.pmid: 10775170