Assessing the potential of an induced-mutation strategy for avermectin overproducers.
Mutant libraries of avermectin-producing Streptomyces avermitilis strains were constructed by different mutagenesis strategies. A metric was applied to assess the mutation spectrum by calculating the distribution of average phenotypic distance of each population. The results showed for the first time that a microgravity environment could introduce larger phenotype distribution and diversity than UV and N-methyl-N-nitro-N-nitrosoguanidine (NTG) could.
Gao
Hong
H
Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing 100190, People's Republic of China.
Liu
Mei
M
Zhuo
Ying
Y
Zhou
Xianlong
X
Liu
Jintao
J
Chen
Difei
D
Zhang
Wenquan
W
Gou
Zhongxuan
Z
Shang
Peng
P
Zhang
Lixin
L
D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000378:metabolism; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016296:Mutagenesis; D009154:Mutation; D010641:Phenotype; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014893:Weightlessness
D003470:Culture Media; D008769:Methylnitronitrosoguanidine; D007559:Ivermectin; C019264:avermectin
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