title: Natural Leishmania infantum infection in Migonemyia migonei (França, 1920) (Diptera:Psychodidae:Phlebotominae) the putative vector of visceral leishmaniasis in Pernambuco State, Brazil.
abstract: A study of the natural infection of phlebotomine sand flies by Leishmania (Leishmania) infantum was conducted in an area of visceral leishmaniasis in São Vicente Férrer, located in the northern part of the Atlantic rain forest region in the State of Pernambuco, Brazil. In a previous study, Migonemyia migonei have been found predominantly in peridomiciles and houses in this endemic area. The analysis of M. migonei, collected by CDC light trap, by multiplex PCR assay coupled to non-isotopic hybridization showed that 2 females out of 50 were infected by L. infantum. This is the first finding of natural infection of M. migonei by L. infantum suggesting that M. migonei may be the vector of L. infantum in areas of visceral leishmaniasis where Lutzomyia longipalpis, the usual vector, is absent.
authors.affiliation: Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, FIOCRUZ, Recife, PE 50670-420, Brazil.
authors.forename: Maria Rosimery
authors.initials: MR
authors.lastname: de Carvalho
authors.forename: Helio França
authors.initials: HF
authors.lastname: Valença
authors.forename: Fernando José
authors.initials: FJ
authors.lastname: da Silva
authors.forename: Daniela
authors.initials: D
authors.lastname: de Pita-Pereira
authors.forename: Thaís
authors.initials: T
authors.lastname: de Araújo Pereira
authors.forename: Constança
authors.initials: C
authors.lastname: Britto
authors.forename: Reginaldo Peçanha
authors.initials: RP
authors.lastname: Brazil
authors.forename: Sinval Pinto
authors.initials: SP
authors.lastname: Brandão Filho
mesh_terms: D000818:Animals; D001938:Brazil; D017931:DNA Primers; D005260:Female; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology*; D018314:Leishmania infantum / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000635:transmission*; D008297:Male; D016133:Polymerase Chain Reaction; D011576:Psychodidae / Q000469:parasitology*
chemical_list: D017931:DNA Primers