title: Neutralizing antibodies to Shiga toxin type 2 (Stx2) reduce colonization of mice by Stx2-expressing Escherichia coli O157:H7.
abstract: Previously, we showed that the Shiga toxin type 2 (Stx2)-expressing Escherichia coli O157:H7 strain 86-24 colonized mice better than did its isogenic stx(2) negative mutant. Here, we confirmed that finding by demonstrating that Stx2 given orally to mice increased the levels of the 86-24 stx(2) mutant shed in feces. Then we assessed the impact of Stx2-neutralizing antibodies, administered passively or generated by immunization with an Stx2 toxoid, on E. coli O157:H7 colonization of mice. We found that such antibodies reduced the E. coli O157:H7 burden in infected mice and, as anticipated, also protected them from weight loss and death.
authors.affiliation: Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
authors.forename: Krystle L
authors.initials: KL
authors.lastname: Mohawk
authors.forename: Angela R
authors.initials: AR
authors.lastname: Melton-Celsa
authors.forename: Cory M
authors.initials: CM
authors.lastname: Robinson
authors.forename: Alison D
authors.initials: AD
authors.lastname: O'Brien
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control*; D019453:Escherichia coli O157 / Q000235:genetics* / Q000276:immunology*; D005243:Feces / Q000737:chemistry; D005260:Female; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011817:Rabbits; D022641:Shiga Toxin 2 / Q000235:genetics / Q000276:immunology*; D014121:Toxoids / Q000276:immunology / Q000627:therapeutic use; D014611:Vaccination; D015431:Weight Loss
chemical_list: D000907:Antibodies, Bacterial; D057134:Antibodies, Neutralizing; D022641:Shiga Toxin 2; D014121:Toxoids
references.citation: Vet Microbiol. 1991 Nov;29(3-4):309-18
references.pmid: 1837635
references.citation: J Infect Dis. 1985 May;151(5):775-82
references.pmid: 3886804
references.citation: Infect Immun. 1999 Jul;67(7):3645-8
references.pmid: 10377152
references.citation: Infect Immun. 2003 Dec;71(12):7129-39
references.pmid: 14638803
references.citation: J Clin Invest. 1993 Sep;92(3):1418-24
references.pmid: 8376595
references.citation: Proc Natl Acad Sci U S A. 2006 May 2;103(18):7082-7
references.pmid: 16641102
references.citation: Infect Immun. 2005 Aug;73(8):4607-13
references.pmid: 16040972
references.citation: Clin Microbiol Rev. 1998 Jan;11(1):142-201
references.pmid: 9457432
references.citation: Gastroenterology. 1994 Feb;106(2):306-17
references.pmid: 8299898
references.citation: J Infect Dis. 1997 Mar;175(3):611-20
references.pmid: 9041333
references.citation: Infect Immun. 2001 Feb;69(2):730-6
references.pmid: 11159961
references.citation: Appl Environ Microbiol. 2009 Aug;75(15):4975-83
references.pmid: 19525268
references.citation: Emerg Infect Dis. 2002 Aug;8(8):833-41
references.pmid: 12141970
references.citation: Appl Environ Microbiol. 2007 Jan;73(1):344-6
references.pmid: 17085690
references.citation: J Biol Chem. 2000 Feb 4;275(5):3713-21
references.pmid: 10652371
references.citation: Infect Immun. 1990 Dec;58(12):3959-65
references.pmid: 2254023
references.citation: Appl Environ Microbiol. 2009 Aug;75(15):5074-81
references.pmid: 19525271
references.citation: Proc Natl Acad Sci U S A. 2006 Jun 20;103(25):9667-72
references.pmid: 16766659
references.citation: Infect Immun. 1996 Jan;64(1):55-60
references.pmid: 8557374
references.citation: Appl Environ Microbiol. 2008 Oct;74(20):6378-84
references.pmid: 18723644
references.citation: Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4868-73
references.pmid: 18332430
references.citation: Infect Immun. 1998 May;66(5):2337-41
references.pmid: 9573126
references.citation: Infect Immun. 2008 Jul;76(7):3054-63
references.pmid: 18443087
references.citation: Clin Infect Dis. 2009 Nov 15;49(10):1480-5
references.pmid: 19827953
references.citation: Clin Microbiol Rev. 2000 Oct;13(4):602-14
references.pmid: 11023960
references.citation: Infect Immun. 2007 Oct;75(10):5043-51
references.pmid: 17698571
references.citation: PLoS Pathog. 2009 Oct;5(10):e1000626
references.pmid: 19834554
references.citation: Am J Epidemiol. 1990 Aug;132(2):239-47
references.pmid: 2196790
references.citation: Nat Rev Microbiol. 2005 Apr;3(4):320-32
references.pmid: 15759043
references.citation: Am J Pathol. 2008 Nov;173(5):1428-39
references.pmid: 18832584
references.citation: Infect Immun. 1992 Feb;60(2):485-90
references.pmid: 1730480
references.citation: Infect Immun. 2006 Aug;74(8):4685-93
references.pmid: 16861656
references.citation: Infect Immun. 1995 Sep;63(9):3739-44
references.pmid: 7642319
references.citation: Clin Infect Dis. 2012 Feb 15;54(4):511-8
references.pmid: 22157169
references.citation: J Infect Dis. 2001 Sep 15;184(6):738-42
references.pmid: 11517435
references.citation: Front Biosci. 1997 Dec 15;2:d635-42
references.pmid: 9392626
references.citation: Clin Vaccine Immunol. 2006 Dec;13(12):1322-7
references.pmid: 17050743
references.citation: Emerg Infect Dis. 1999 Sep-Oct;5(5):607-25
references.pmid: 10511517
references.citation: Clin Vaccine Immunol. 2008 Feb;15(2):359-66
references.pmid: 18003816
references.citation: Vet Microbiol. 2003 May 29;93(3):207-22
references.pmid: 12695045
references.citation: Microb Pathog. 2010 Mar-Apr;48(3-4):131-42
references.pmid: 20096770
references.citation: Infect Immun. 2006 Feb;74(2):869-75
references.pmid: 16428729
references.citation: Infect Immun. 2004 Jul;72(7):3914-24
references.pmid: 15213135
references.citation: J Infect Dis. 2001 Jan 15;183(2):347-350
references.pmid: 11110647
references.citation: Infect Immun. 2006 Feb;74(2):1255-65
references.pmid: 16428775
references.citation: J Biol Chem. 2002 Jan 25;277(4):2876-85
references.pmid: 11704679
references.citation: J Biol Chem. 2004 Aug 6;279(32):33751-8
references.pmid: 15173179