title: Initiation of the TORC1-regulated G0 program requires Igo1/2, which license specific mRNAs to evade degradation via the 5'-3' mRNA decay pathway.
abstract: Eukaryotic cell proliferation is controlled by growth factors and essential nutrients, in the absence of which cells may enter into a quiescent (G(0)) state. In yeast, nitrogen and/or carbon limitation causes downregulation of the conserved TORC1 and PKA signaling pathways and, consequently, activation of the PAS kinase Rim15, which orchestrates G(0) program initiation and ensures proper life span by controlling distal readouts, including the expression of specific genes. Here, we report that Rim15 coordinates transcription with posttranscriptional mRNA protection by phosphorylating the paralogous Igo1 and Igo2 proteins. This event, which stimulates Igo proteins to associate with the mRNA decapping activator Dhh1, shelters newly expressed mRNAs from degradation via the 5'-3' mRNA decay pathway, thereby enabling their proper translation during initiation of the G(0) program. These results delineate a likely conserved mechanism by which nutrient limitation leads to stabilization of specific mRNAs that are critical for cell differentiation and life span.
authors.affiliation: Department of Medicine, Division of Biochemistry, University of Fribourg, CH-1700 Fribourg, Switzerland.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Talarek
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Cameroni
authors.forename: Malika
authors.initials: M
authors.lastname: Jaquenoud
authors.forename: Xuan
authors.initials: X
authors.lastname: Luo
authors.forename: Séverine
authors.initials: S
authors.lastname: Bontron
authors.forename: Soyeon
authors.initials: S
authors.lastname: Lippman
authors.forename: Geeta
authors.initials: G
authors.lastname: Devgan
authors.forename: Michael
authors.initials: M
authors.lastname: Snyder
authors.forename: James R
authors.initials: JR
authors.lastname: Broach
authors.forename: Claudio
authors.initials: C
authors.lastname: De Virgilio
mesh_terms: D020413:3' Untranslated Regions; D002244:Carbon / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000172:deficiency; D006360:Heat-Shock Proteins / Q000235:genetics; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D020871:RNA Stability; D012331:RNA, Fungal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016192:Resting Phase, Cell Cycle / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D020413:3' Untranslated Regions; D018797:Cell Cycle Proteins; C499528:HSP26 protein, S cerevisiae; D006360:Heat-Shock Proteins; C549818:Igo1 protein, S cerevisiae; C549819:Igo2 protein, S cerevisiae; D012331:RNA, Fungal; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D002244:Carbon; D011494:Protein Kinases; C410736:Rim15 protein, S cerevisiae; C445083:DHH1 protein, S cerevisiae; D053487:DEAD-box RNA Helicases; D005947:Glucose; D009584:Nitrogen
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