Concordance and population studies along with stutter and peak height ratio analysis for the PowerPlex ® ESX 17 and ESI 17 Systems.
The PowerPlex(®) ESX 17 and ESI 17 Systems for short tandem repeat (STR) amplification were developed by the Promega Corporation to meet the European Network of Forensic Science Institutes (ENFSI) and the European DNA Profiling (EDNAP) Group recommendations for increasing the number of STR loci included in the European Standard Set (ESS). The PowerPlex ESX 17 and ESI 17 Systems utilize different PCR primer combinations to co-amplify the following 17 loci: D1S1656, D2S441, D2S1338, D3S1358, D8S1179, D10S1248, D12S391, D16S539, D18S51, D19S433, D21S11, D22S1045, FGA, TH01, vWA, SE33, and the sex-typing locus amelogenin. A total of 1443 U.S. population samples were evaluated with pre-commercialization versions of both kits. Stutter and heterozygote peak height ratios have been used to characterize kit performance. Typing results have been used to estimate the match probabilities provided by the chosen loci as well as in concordance studies. Full concordance between the typing results for the two kits was observed in 99.994% (49,055 out of 49,062) STR allele calls compared. All genotyping discrepancies were confirmed by DNA sequence analysis. As a result of these comparisons, a second forward primer for the D22S1045 locus has been added to the PowerPlex ESX 17 System to address a primer binding site mutation and the D1S1656 locus reverse primer in the PowerPlex ESI 17 System was modified to eliminate an amplification-efficiency reducing primer dimer.
Hill
Carolyn R
CR
National Institute of Standards and Technology, Chemical Science and Technology Laboratory, Gaithersburg, MD 20899-8312, USA. becky.hill@nist.gov
Duewer
David L
DL
Kline
Margaret C
MC
Sprecher
Cynthia J
CJ
McLaren
Robert S
RS
Rabbach
Dawn R
DR
Krenke
Benjamin E
BE
Ensenberger
Martin G
MG
Fulmer
Patricia M
PM
Storts
Douglas R
DR
Butler
John M
JM
D016172:DNA Fingerprinting / Q000295:instrumentation*; D017931:DNA Primers; D030561:Databases, Nucleic Acid; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D044469:Racial Groups / Q000235:genetics; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences
D017931:DNA Primers