title: Oligosaccharide-assisted direct immunosensing of small molecules.
abstract: "Sandwich-type" noncompetitive (immunometric) assays allow for high-sensitivity high-throughput macromolecule sensing and determination but cannot be used on small molecules (haptens). Here, we isolated single-chain Fv fragments from a phage-display library, which bound to complexes of particular haptens (vitamin D and A derivatives) with immobilized beta-cyclodextrin or beta-maltosyl residues, and formed ternary complexes. These scFvs enabled novel "semisandwich-type" immunometric assays of haptens with nanomole-range sensitivities.
authors.affiliation: Kobe Pharmaceutical University, 4-19-1, Motoyama-Kitamachi, Higashinada-ku, Kobe 658-8558, Japan. no-kobay@kobepharma-u.ac.jp
authors.forename: Norihiro
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Oyama
authors.forename: Iwao
authors.initials: I
authors.lastname: Suzuki
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kato
authors.forename: Takeo
authors.initials: T
authors.lastname: Umemura
authors.forename: Junichi
authors.initials: J
authors.lastname: Goto
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002236:Carbohydrate Conformation; D002417:Cattle; D006241:Haptens / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D057166:High-Throughput Screening Assays; D007118:Immunoassay / Q000379:methods*; D008958:Models, Molecular; D009844:Oligosaccharides / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D057127:Single-Chain Antibodies / Q000276:immunology / Q000378:metabolism; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000378:metabolism
chemical_list: D006241:Haptens; D009844:Oligosaccharides; D057127:Single-Chain Antibodies; D047392:beta-Cyclodextrins; D012710:Serum Albumin, Bovine