Host-guest chemistry of dendrimer-drug complexes. 4. An in-depth look into the binding/encapsulation of guanosine monophosphate by dendrimers.
In the present study, we investigated the host-guest chemistry of dendrimer/guanosine monophosphate (GMP) and present an in-depth look into the binding/encapsulation of GMP by dendrimers using NMR studies. (1)H NMR spectra showed a significant downfield shift of methylene protons in the outmost layer of the G5 dendrimer, indicating the formation of ion pairs between cationic amine groups of dendrimer and anionic phosphate groups of GMP. Chemical shift titration results showed that the binding constant between G5 dendrimer and GMP is 17,400 M(-1) and each G5 dendrimer has 107 binding sites. The binding of GMP to dendrimers prevents its aggregation in aqueous solutions and thereby enhances its stability. Nuclear Overhauser effect measurements indicated that a GMP binding and encapsulation balance occurs on the surface and in the interior of dendrimer. The binding/encapsulation transitions can be easily tailored by altering the surface and interior charge densities of the dendrimer. All these findings provide a new insight into the host-guest chemistry of dendrimer/guest complexes and may play important roles in the study of dendrimer/DNA aggregates by a "bottom-up" strategy.
Hu
Jingjing
J
CAS Key Laboratory of Soft Matter Chemistry, School of Chemistry and Material Science, University of Science and Technology of China, Hefei, Anhui, 230026, People's Republic of China.
Fang
Min
M
Cheng
Yiyun
Y
Zhang
Jiahai
J
Wu
Qinglin
Q
Xu
Tongwen
T
D001665:Binding Sites; D050091:Dendrimers / Q000737:chemistry*; D006157:Guanosine Monophosphate / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D004364:Pharmaceutical Preparations / Q000737:chemistry*
D050091:Dendrimers; D004364:Pharmaceutical Preparations; D006157:Guanosine Monophosphate