[No correlation between the sensitivity to 5-aza-dC and the global DNA methylation level in hepatocellular carcinoma cells].
OBJECTIVE
To compare the sensitivity of different hepatocellular carcinoma (HCC) cell lines (HepG2, QGY7701, HepG2.2.15) and the normal liver cell line L02 to 5-aza-dC, an DNA methyltransferase inhibitor, and to explore the relationship between global DNA methylation level and the sensitivity to 5-aza-dC.


METHODS
HepG2, QGY7701, HepG2.2.15 and L02 cells were treated with 5-aza-dC at different concentration, cell proliferation was measured by MTT method, cell apoptosis was detected by measuring caspase 3 activity and cellular DNA fragmentation ELISA.


RESULTS
Compared to HepG2 and QGY7701 cells, HepG2.2.15 were less sensitive to the treatment of 5-aza-dC; the normal liver cell line L02 was less sensitive to 5-aza-dC than the HCC cell lines.


CONCLUSIONS
The sensitivity to 5-aza-dC of HCC cell lines and normal liver cells is not correlated with the global DNA methylation level.
Li
Xiao-ping
XP
Department of Gastroenterology, the Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing, China.
Huang
Ai-long
AL
Yang
Mei
M
D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D019175:DNA Methylation / Q000187:drug effects*; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors*; D000077209:Decitabine; D056945:Hep G2 Cells; D006801:Humans
D000077209:Decitabine; D015254:DNA Modification Methylases; D053148:Caspase 3; D001374:Azacitidine