[Identification of genes regulated by HBV preS1-transactivated protein 2 binding protein 1].
OBJECTIVE
To identify genes regulated by HBV preS1-transactivated protein 2 binding protein 1 (PS1TP2BP1).


METHODS
PS1TP2BP1 gene was amplified by polymerase chain reaction (PCR) technique and cloned into the eukaryotic expression vector pcDNA 3.1/my-c-His A. The mRNAs isolated from HepG2 cells transfected recombinant eukaryotic expression vector pcDNA 3.1/myc-HisA-PS1TP2BP1 and pcDNA 3.1/myc-HisA empty vector were used to construct subtractive library. The differentially expressed genes were identified and analyzed.


RESULTS
35 differentially expressed clones were obtained. Colony PCR identified 15 clones with 200-1000 bp inserts. Sequence analysis identified 15 differentially expressed genes.


CONCLUSIONS
This study provides data for further characterize the function of PS1TP2BP1.
Zhang
Huan-hu
HH
Hepatopathy Center, Second Hospital, Shanxi Medical University, Taiyuan, China.
Wang
Dan-qiong
DQ
Cheng
Jun
J
Zhao
Jie
J
Zhao
Xin-kai
XK
Gao
Hong
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins; D003001:Cloning, Molecular; D015723:Gene Library; D005822:Genetic Vectors; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus; D006801:Humans; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*
D002352:Carrier Proteins; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D015534:Trans-Activators; C050806:presurface protein 1, hepatitis B surface antigen