miR-20a promotes proliferation and invasion by targeting APP in human ovarian cancer cells.
MicroRNAs (miRNAs) are emerging as a class of small regulated RNAs, and the alterations of miRNAs are implicated in the initiation and progression of human cancers. Our study shows that inhibition of miR-20a in OVCAR3 ovarian cancer cell line could suppress, whereas overexpression of miR-20a could enhance cell long-term proliferation and invasion. We also confirmed amyloid precursor protein (APP) as a direct target gene of miR- 20a. Furthermore, suppression of APP expression could also promote ovarian cancer cell proliferation and invasion, which is consistent with the results of miR-20a overexpression. Therefore, we concluded that the regulation of APP is an important mechanism for miR-20a to promote proliferation and invasion in ovarian cancer cells.
Fan
Xingxing
X
Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin 300070, China.
Liu
Yankun
Y
Jiang
Jiechun
J
Ma
Zhuoya
Z
Wu
Haidong
H
Liu
Tao
T
Liu
Min
M
Li
Xin
X
Tang
Hua
H
D016564:Amyloid beta-Protein Precursor / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D035683:MicroRNAs / Q000494:pharmacology*; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms / Q000473:pathology*
D016564:Amyloid beta-Protein Precursor; C534451:MIRN20a microRNA, human; D035683:MicroRNAs