DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases.
DNA-scaffolded oligodeoxyriboside fluorophores (ODFs) were used as the reporters in turn-on sensing of enzymatic bond-cleaving activity. A tetramer ODF of pyrene deoxynucleosides displayed high quenching efficiency when conjugated via ester linkages with a dabcyl quencher, and yielded large signal increases with several enzymes in vitro and in intact human cells.
Dai
Nan
N
Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
Teo
Yin Nah
YN
Kool
Eric T
ET
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry; D003853:Deoxyribonucleosides / Q000737:chemistry* / Q000378:metabolism; D004950:Esterases / Q000032:analysis / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008049:Lipase / Q000032:analysis / Q000378:metabolism*; D015394:Molecular Structure
D003853:Deoxyribonucleosides; D005456:Fluorescent Dyes; D004247:DNA; D004950:Esterases; D008049:Lipase
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