Water-in-ionic liquid microemulsion-based organogels as novel matrices for enzyme immobilization.
The use of water-in-ionic liquid microemulsion-based organogels (w/IL MBGs) as novel supports for the immobilization of lipase B from Candida antarctica and lipase from Chromobacterium viscosum was investigated. These novel lipase-containing w/IL MBGs can be effectively used as solid phase biocatalysts in various polar and non-polar organic solvents or ILs, exhibiting up to 4.4-fold higher esterification activity compared to water-in-oil microemulsion-based organogels. The immobilized lipases retain their activity for several hours at 70 degrees C, while their half life time is up to 25-fold higher compared to that observed in w/IL microemulsions. Fourier-transform infrared spectroscopy data indicate that immobilized lipases adopt a more rigid structure, referring to the structure in aqueous solution, which is in correlation with their enhanced catalytic behavior observed.
Pavlidis
Ioannis V
IV
Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece.
Tzafestas
Kyriakos
K
Stamatis
Haralambos
H
D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods*; D004655:Emulsions / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D017743:Equipment Reuse; D005583:Fourier Analysis; D005656:Fungal Proteins; D005782:Gels / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D013696:Temperature
D004655:Emulsions; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D005782:Gels; D052578:Ionic Liquids; D008049:Lipase; C431349:lipase B, Candida antarctica; C454481:lipase, Chromobacterium viscosum