Characterization of antibody responses to the Sj23 antigen of Schistosoma japonicum after infection and immunization.
Sj23, a member of the tetraspanin protein family, is a 23-kDa surface-exposed protein of Schistosoma japonicum and expressed in all infective parasite stages, which has been regarded as a potential candidate in vaccine development for schistosomiasis. In this study, we found that, in the BALB/c mouse model, Sj23 elicited rapid humoral responses after parasite infection and the dominant antibodies were of IgG2a subclass. Immunization with Sj23 by priming with recombinant SFV RNA virus particles followed by a boost with recombinant protein also generated strong IgG2a responses which did not provide protection against challenge with cercariae. Our data indicated that one of the functions of Sj23 of S. japonicum is to facilitate parasite immune regulation. Sj23 antigen-based vaccine may require strong adjuvant that can drive IgG1 responses which are more critical in resistance to helminth infection.
Jiang
Ning
N
Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China.
Cai
Pengfei
P
Yin
Jigang
J
Hao
Lili
L
Lu
Huijun
H
Wang
Xinrui
X
Wang
Heng
H
Chen
Qijun
Q
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis / Q000096:biosynthesis*; D000947:Antigens, Helminth / Q000276:immunology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015801:Helminth Proteins / Q000276:immunology*; D007074:Immunoglobulin G; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D011817:Rabbits; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000517:prevention & control; D013047:Specific Pathogen-Free Organisms; D014614:Vaccines, Synthetic
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G; D008565:Membrane Proteins; D014614:Vaccines, Synthetic; C072633:Sj23 antigen, Schistosoma japonicum