title: The route of priming influences the ability of respiratory virus-specific memory CD8+ T cells to be activated by residual antigen.
abstract: After respiratory virus infections, memory CD8+ T cells are maintained in the lung airways by a process of continual recruitment. Previous studies have suggested that this process is controlled, at least in the initial weeks after virus clearance, by residual antigen in the lung-draining mediastinal lymph nodes (MLNs). We used mouse models of influenza and parainfluenza virus infection to show that intranasally (i.n.) primed memory CD8+ T cells possess a unique ability to be reactivated by residual antigen in the MLN compared with intraperitoneally (i.p.) primed CD8+ T cells, resulting in the preferential recruitment of i.n.-primed memory CD8+ T cells to the lung airways. Furthermore, we demonstrate that the inability of i.p.-primed memory CD8+ T cells to access residual antigen can be corrected by a subsequent i.n. virus infection. Thus, two independent factors, initial CD8+ T cell priming in the MLN and prolonged presentation of residual antigen in the MLN, are required to maintain large numbers of antigen-specific memory CD8+ T cells in the lung airways.
authors.affiliation: Trudeau Institute, Saranac Lake, NY 12983, USA. stakamura@immunol.med.kindai.ac.jp
authors.forename: Shiki
authors.initials: S
authors.lastname: Takamura
authors.forename: Alan D
authors.initials: AD
authors.lastname: Roberts
authors.forename: Dawn M
authors.initials: DM
authors.lastname: Jelley-Gibbs
authors.forename: Susan T
authors.initials: ST
authors.lastname: Wittmer
authors.forename: Jacob E
authors.initials: JE
authors.lastname: Kohlmeier
authors.forename: David L
authors.initials: DL
authors.lastname: Woodland
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000821:virology*; D002465:Cell Movement; D045142:Cross-Priming / Q000276:immunology*; D000939:Epitopes; D007156:Immunologic Memory / Q000276:immunology*; D007274:Injections, Intraperitoneal; D008168:Lung / Q000276:immunology* / Q000821:virology; D008198:Lymph Nodes / Q000276:immunology / Q000821:virology; D008482:Mediastinum / Q000821:virology; D051379:Mice; D009975:Orthomyxoviridae / Q000276:immunology*; D029082:Sendai virus / Q000276:immunology*; D013045:Species Specificity
chemical_list: D000956:Antigens, Viral; D000939:Epitopes
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