[Prevention of ulcerogenesis at experimental pancreatitis].
OBJECTIVE
We wanted to study the mechanisms of "Dimefosfon" influence on ulcerogenesis prevention in acute pancreatitis.


METHODS
The study was conducted on 36 dogs. We were modeling of edematous form of pancreatitis: fulfilled the median laparotomy, punctured of the gall bladder bile were taken, it was administered at 5 points of the pancreas parenchyma to 0.5 ml. The animals of experimental group on a daily basis once a day for 5 days were administered intravenously 15% solution of dimefosfon the rate of 50 mg/kg body weight. In the tissues of the duodenum was examined the intensity of lipid peroxidation, determined indicators of hypoxia, transcapillar exchange and bioenergy, and conducted their microscopic examination.


RESULTS
Dimefosfon helped to reduce the severity of inflammation in the pancreas parenchyma, as well as the destructive changes in the duodenum. Dimefosfon effectiveness in correction of microcirculation, trophic system and bio-energy disorders in the tissues of intestine was shown by the third day of the drug using. At this period the capillary filtrate, the diffusion coefficient of oxygen, the rate of electrogenesis duodenal tissues significantly exceeded results of the control group study. Indicator protein loss wasn't significantly different from controls. Efficiency of dimefosfon in correction indicators of hypoxia was also seen with the third day of therapy. In duodenal tissue during this period there was a decrease of lactate and pyruvic acid as compared with control. Significant changes in indicators of intensity of lipid peroxidation and the activity of phosfolipase A2 in duodenal tissues were observed on the third day of dimefosfon using. At this stage of observation resultes were compared with the control and had decreased of malondialdehyde content and activity of phosfolipase A2. Catalase activity was lower compared with the control and did not differ from the norm.


CONCLUSIONS
One of the ulcerogenesis mechanisms in duodenum in acute pancreatitis is a violation of microcirculation, deterioration trophics tissues of the intestinal wall that contribute to the launch of free radical reactions and lead to membranodestructive processes in the intestinal mucosa. Dimefosfon has cytoprotect effect as a result of decreased activity of lipid peroxidation and restoration of microcirculation and trophics tissues of intestinal wall. Positive therapeutic effect of the drug was observed with the third day of therapy.
Mosina
L M
LM
Tarasova
T V
TV
Avdeĭkina
O I
OI
Arsent'eva
E V
EV
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D004285:Dogs; D004381:Duodenal Ulcer / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D004386:Duodenum / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009943:Organophosphorus Compounds; D010179:Pancreas / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D010195:Pancreatitis / Q000097:blood / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology
D000897:Anti-Ulcer Agents; D009943:Organophosphorus Compounds; C049638:dimephosphon