title: Characterization of abdominal appendages in the sawfly, Athalia rosae (Hymenoptera), by morphological and gene expression analyses.
abstract: Larvae of the sawfly, Athalia rosae, have remarkable abdominal prolegs. We analyzed the morphogenesis of appendages and the expression of decapentaplegic and Distal-less genes during embryonic development to characterize the origin of prolegs. Proleg primordia in abdominal segments A1-A9 appeared shortly after the inner lobes (endites) of gnathal appendages were formed. These were located on the ventral plates, medioventral to the appendages of the other segments in light of serial homology. Nothing was seen where the main axis of the appendage should develop in abdominal segments. The primordia in A1 and A9 disappeared before larval hatching. Anal prolegs appeared separate from cerci, the main axes of appendages, which were formed temporarily in A11. The expression of decapentaplegic, which reflects the primary determination of appendages, was detected in the lateral juxtaposition with the prolegs. Distal-less was expressed in the main axes of appendages, protruding endites and the cerci, but not in prolegs and anal prolegs or the gnathal endites which do not protrude. These findings suggest a possibility that the abdominal and anal prolegs of A. rosae are outgrowths of ventral plates which derived from coxopodal elements, but not main axes of appendages.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Oka
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Yoshiyama
authors.forename: Koji
authors.initials: K
authors.lastname: Tojo
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Machida
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Hatakeyama
mesh_terms: D000005:Abdomen / Q000196:embryology; D000818:Animals; D019521:Body Patterning; D004625:Embryo, Nonmammalian / Q000378:metabolism / Q000648:ultrastructure; D005121:Extremities / Q000196:embryology; D015870:Gene Expression; D006927:Hymenoptera / Q000196:embryology* / Q000235:genetics / Q000648:ultrastructure*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000254:growth & development
chemical_list: D019476:Insect Proteins
references.citation: Dev Genes Evol. 2006 Nov;216(11):655-65
references.pmid: 16773338
references.citation: J Morphol. 2005 Nov;266(2):182-207
references.pmid: 16155878
references.citation: Dev Genes Evol. 2001 Oct;211(10):486-92
references.pmid: 11702198
references.citation: Dev Biol. 2005 Oct 1;286(1):57-77
references.pmid: 16112665
references.citation: Nature. 1994 Dec 1;372(6505):458-61
references.pmid: 7840822
references.citation: J Morphol. 2009 Aug;270(8):984-95
references.pmid: 19274641
references.citation: Evol Dev. 2004 Nov-Dec;6(6):402-10
references.pmid: 15509222
references.citation: Evol Dev. 2002 Nov-Dec;4(6):459-99
references.pmid: 12492146
references.citation: Dev Genes Evol. 2004 Mar;214(3):128-33
references.pmid: 14872342