Chocolate matrix factors modulate the pharmacokinetic behavior of cocoa flavan-3-ol phase II metabolites following oral consumption by Sprague-Dawley rats.
The impact of carbohydrates and milk on the bioavailability of catechin (C) and epicatechin (EC) from chocolate has been previously studied. However, little data exist regarding potential modulation of the phase II metabolism by these chocolate matrix factors. The objectives of this study were to assess the impact of matrix composition on qualitative and quantitative profiles of circulating catechins and their metabolites following administration of commercially relevant chocolate confections. Sprague-Dawley rats were administered 1.5 g of a confection (reference dark, high sucrose, or milk chocolate) by intragastric gavage, and plasma samples were collected over 8 h. High-performance liquid chromatography-mass spectrometry analysis was performed to quantify C, EC, and their metabolites. The predominant metabolites were O-glucuronides (two metabolites) and O-Me-O-glucuronides (three metabolites). Plasma concentrations of metabolites were generally the highest for high sucrose treatment and lowest for milk treatment, while the reference dark treatment generally resulted in intermediate concentrations. The O-Me-(+/-)-C/EC-O-beta-glucuronide (peak 4) was significantly higher for the high sucrose treatment (2325 nM h) versus the milk treatment (1300 nM h). Additionally, C(MAX) values for (+/-)-C/EC-O-beta-glucuronide (peak 3) and two O-Me-(+/-)-C/EC-O-beta-glucuronides (peaks 4 and 6) were significantly higher for the high sucrose treatment (4012, 518, and 2518 nM, respectively) versus the milk treatment (2590, 240, and 1670 nM, respectively). Milk and sucrose appear to modulate both metabolism and plasma pharmacokinetics and, to a lesser extent, the overall bioavailability of catechins from chocolate confections.
Neilson
Andrew P
AP
Department of Food Science, Purdue University, West Lafayette, Indiana 47907, USA.
Sapper
Teryn N
TN
Janle
Elsa M
EM
Rudolph
Ralf
R
Matusheski
Nathan V
NV
Ferruzzi
Mario G
MG
D000284:Administration, Oral; D000818:Animals; D003059:Cocos / Q000737:chemistry*; D005419:Flavonoids / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005419:Flavonoids; D010936:Plant Extracts; C404987:flavan-3-ol
Free Radic Biol Med. 2002 Dec 15;33(12):1693-702
12488137
Br J Nutr. 2008 Oct;100(4):846-51
18257943
Am J Clin Nutr. 2005 Mar;81(3):611-4
15755830
Hypertension. 2005 Aug;46(2):398-405
16027246
J Agric Food Chem. 2001 Dec;49(12):6050-6
11743807
Br J Nutr. 2000 Nov;84(5):673-80
11177180
J Agric Food Chem. 2009 Oct 28;57(20):9418-26
19780539
J Am Coll Nutr. 2004 Jun;23(3):197-204
15190043
Br J Nutr. 2002 Jan;87(1):31-7
11895312
J Agric Food Chem. 2006 May 31;54(11):4057-61
16719534
Free Radic Biol Med. 2004 Nov 1;37(9):1351-9
15454274
Br J Nutr. 2005 Aug;94(2):170-81
16115350
J Nutr. 2008 Aug;138(8):1535S-1542S
18641203
Nature. 2003 Aug 28;424(6952):1013
12944955
Am J Clin Nutr. 2001 Nov;74(5):596-602
11684527
Am J Clin Nutr. 2009 Jun;89(6):1784-91
19403635
J Food Sci. 2007 Apr;72(3):S230-3
17995820
J Agric Food Chem. 2002 Aug 14;50(17):4852-60
12166971
Ann Nutr Metab. 2007;51(6):493-8
18032884
J Nutr. 2000 Aug;130(8S Suppl):2115S-9S
10917932
Am J Clin Nutr. 2004 Dec;80(6):1558-64
15585768
J Agric Food Chem. 2005 Aug 10;53(16):6190-4
16076092
J Agric Food Chem. 2007 Apr 18;55(8):2841-7
17362030
J Nutr. 2001 Nov;131(11):2885-91
11694613
J Agric Food Chem. 2003 Dec 3;51(25):7292-5
14640573
Biosci Biotechnol Biochem. 2000 Dec;64(12):2581-7
11210120
J Am Coll Cardiol. 2005 Oct 4;46(7):1276-83
16198843
Free Radic Res. 2006 Oct;40(10):1029-34
17015247
Am J Clin Nutr. 2008 Jul;88(1):58-63
18614724
Biosci Biotechnol Biochem. 1999 Jun;63(6):973-7
10427682
Proc Natl Acad Sci U S A. 2006 Jan 24;103(4):1024-9
16418281
J Mass Spectrom. 2003 Jan;38(1):35-42
12526004
Am J Clin Nutr. 2007 Mar;85(3):709-17
17344491
Life Sci. 2003 Jul 4;73(7):857-69
12798412
Nature. 2003 Dec 18;426(6968):787-8; discussion 788
14685225
Circulation. 2007 Nov 20;116(21):2376-82
17984375
J Nutr. 2008 Sep;138(9):1671-6
18716168
Drug Metab Dispos. 1999 Feb;27(2):309-16
9929521