title: Comparative proteomics analysis of differentially expressed proteins in soybean cell wall during flooding stress.
abstract: Flooding is a major problem for soybean crop as it reduces the growth and grain yield. To investigate the function of the soybean cell wall in the response to flooding stress, cell wall proteins were analyzed. Cell wall proteins from roots and hypocotyls of soybeans, which were germinated for 2 days and subjected to 2 days of flooding, were purified, separated by two-dimensional polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue. Sixteen out of 204 cell wall proteins showed responses to flooding stress. Of these, two lipoxygenases, four germin-like protein precursors, three stem 28/31 kDa glycoprotein precursors, and one superoxide dismutase [Cu-Zn] were downregulated. A copper amine oxidase was found to have shifted from the basic to acidic zone following flooding stress. Based on these results, it was confirmed by the lignin staining that the lignification was suppressed in the root of soybean under the flooding stress. These results suggest that the roots and hypocotyls of soybean caused the suppression of lignification through decrease of these proteins by downregulation of reactive oxygen species and jasmonate biosynthesis under flooding stress.
authors.affiliation: National Institute of Crop Science, Kannondai 2-1-18, Tsukuba, 305-8518, Japan. skomatsu@affrc.go.jp
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
authors.forename: Yukito
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Keito
authors.initials: K
authors.lastname: Nishizawa
authors.forename: Yohei
authors.initials: Y
authors.lastname: Nanjo
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D000085:Acetates / Q000378:metabolism; D000595:Amino Acid Sequence; D002473:Cell Wall / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D015536:Down-Regulation; D055868:Floods; D018506:Gene Expression Regulation, Plant; D018546:Hypocotyl / Q000737:chemistry / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism; D030262:Soybean Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D013025:Glycine max / Q000166:cytology*; D013312:Stress, Physiological
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D017382:Reactive Oxygen Species; D030262:Soybean Proteins; C072239:methyl jasmonate