A Bayesian statistical analysis of mouse dermal tumor promotion assay data for evaluating cigarette smoke condensate.
The mouse dermal assay has long been used to assess the dermal tumorigenicity of cigarette smoke condensate (CSC). This mouse skin model has been developed for use in carcinogenicity testing utilizing the SENCAR mouse as the standard strain. Though the model has limitations, it remains as the most relevant method available to study the dermal tumor promoting potential of mainstream cigarette smoke. In the typical SENCAR mouse CSC bioassay, CSC is applied for 29 weeks following the application of a tumor initiator such as 7,12-dimethylbenz[a]anthracene (DMBA). Several endpoints are considered for analysis including: the percentage of animals with at least one mass, latency, and number of masses per animal. In this paper, a relatively straightforward analytic model and procedure is presented for analyzing the time course of the incidence of masses. The procedure considered here takes advantage of Bayesian statistical techniques, which provide powerful methods for model fitting and simulation. Two datasets are analyzed to illustrate how the model fits the data, how well the model may perform in predicting data from such trials, and how the model may be used as a decision tool when comparing the dermal tumorigenicity of cigarette smoke condensate from multiple cigarette types. The analysis presented here was developed as a statistical decision tool for differentiating between two or more prototype products based on the dermal tumorigenicity.
Kathman
Steven J
SJ
RJ Reynolds Tobacco Co, Research and Development, Winston-Salem, NC 27102, USA. kathmas@rjrt.com
Potts
Ryan J
RJ
Ayres
Paul H
PH
Harp
Paul R
PR
Wilson
Cody L
CL
Garner
Charles D
CD
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D001499:Bayes Theorem; D015197:Carcinogenicity Tests / Q000379:methods*; D002273:Carcinogens / Q000633:toxicity; D005260:Female; D051379:Mice; D018632:Mice, Inbred SENCAR; D008954:Models, Biological; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000453:epidemiology; D014026:Nicotiana / Q000633:toxicity*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
D002273:Carcinogens; D014028:Tobacco Smoke Pollution; D015127:9,10-Dimethyl-1,2-benzanthracene