title: Intrahepatic growth and maturation of Gnathostoma turgidum in the natural definitive opossum host, Didelphis virginiana.
abstract: Gnathostoma turgidum is a gastric nematode parasite of opossums found in the Americas. We recently found that G. turgidum juveniles appear in the liver of the opossums where they become mature adults and almost synchronously move to the stomach during certain months of the year, suggesting the importance of the liver for the growth and maturation of this species in the final hosts. In this study we attempted to detect G. turgidum larvae in the liver of opossums, Didelphis virginiana that are the natural final hosts. The results show that tiny (<3mm in length) third stage larvae (L3) appeared in the liver of opossums around November and December. Also in the liver, we found large L3 of up to about 10mm in length together with juveniles and mature adults from February to March. In spite of their length, large L3 have 4 rows of hooklets, and their gonads remained undeveloped. Morphological features of the small and large L3 of G. turgidum are described including scanning electron microscope images. The seasonal switching of the several growth stages of G. turgidum from small L3 to adult worms in the liver and eventual migration to the stomach in opossums suggests the unique feature of G. turgidum utilizing the liver as the maturation site.
authors.affiliation: Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México.
authors.forename: Sylvia Páz
authors.initials: SP
authors.lastname: Díaz-Camacho
authors.forename: Francisco
authors.initials: F
authors.lastname: Delgado-Vargas
authors.forename: Kaethe
authors.initials: K
authors.lastname: Willms
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: de la Cruz-Otero
authors.forename: José
authors.initials: J
authors.lastname: Guadalupe Rendón-Maldonado
authors.forename: Lilia
authors.initials: L
authors.lastname: Robert
authors.forename: Silvia
authors.initials: S
authors.lastname: Antuna
authors.forename: Yukifumi
authors.initials: Y
authors.lastname: Nawa
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000032:analysis / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D048293:Didelphis / Q000469:parasitology*; D006039:Gnathostoma / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D006790:Host-Parasite Interactions; D007814:Larva / Q000254:growth & development / Q000648:ultrastructure; D008099:Liver / Q000469:parasitology*; D008855:Microscopy, Electron, Scanning; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D017205:Spirurida Infections / Q000469:parasitology / Q000662:veterinary*; D013270:Stomach / Q000469:parasitology
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer