title: [Effect of ultrasound microbubble carrying herpes simplex virus thymidine kinase on hepatocellular carcinoma in mice].
abstract: OBJECTIVE
  To observe the effect of ultrasound microbubble carrying herpes simplex virus thymidine kinase hepatocellular carcinoma in mice.
  
  
  METHODS
  Kunming mice were inoculated subcutaneously with H22 tumor cells. 40 male mice bearing subcutaneous hepatoma were randomized into 4 groups: PBS (group A), HSV1-TK (group B), HSV1-TK (group C), and microbubble carrying HSV1-TK (group D) were injected into the tail vein every 3 days. Mice in group C and D were exposed to ultrasound. The expression of TK protein was detected by western blot. Ganciclovir (GCV) was intraperitoneally injected at a dose of 100 mg x kg (-1) x d(-1) in group B, group C and group D. The tumor size was measured every 2 days.
  
  
  RESULTS
  TK gene could be injected precisely into hepatocellular carcinoma with ultrasound monitor, and the expression of TK protein was found in all 4 groups. Expression in group D was higher than others (P < 0.05). The rate of tumor growth inhibition were 0 in group A, 3.90%+/-1.80% in group B, 22.70%+/-2.86% in group C, 41.25%+/-3.20% in group D (group B vs group C, P < 0.05; group D vs group C, P < 0.05; group D vs group B, P < 0.05).
  
  
  CONCLUSIONS
  Ultrasound microbubble not only improve target gene therapy, but also enhance transfection efficiency.
authors.affiliation: Department of Hepatobiliary Surgery, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Shi-ji
authors.initials: SJ
authors.lastname: Zhou
authors.forename: Chan-an
authors.initials: CA
authors.lastname: Liu
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Gong
authors.forename: Zuo-jin
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
authors.forename: Sheng-wei
authors.initials: SW
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Ai
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000628:therapy*; D045744:Cell Line, Tumor; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy; D008113:Liver Neoplasms / Q000628:therapy*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D045423:Microbubbles; D018139:Simplexvirus / Q000235:genetics / Q000378:metabolism; D013937:Thymidine Kinase / Q000235:genetics*; D016896:Treatment Outcome; D014465:Ultrasonics
chemical_list: D013937:Thymidine Kinase