Platform technology to identify potential disease markers and establish heritability and environmental determinants of the human serum N-glycome.
Glycomics has been proven to be challenging when compared to genomics, transcriptomics, or proteomics. Understanding glycans is difficult as they have a non-template driven biosynthesis and their structural characterization requires highly efficient techniques. This review will describe the robotic platform developed in our laboratory for high-throughput analysis of N-glycans, and some of the advances obtained in the Glyco-biomarker field obtained in the last three years, including the first Genome-Wide Association Study showing a direct link between DNA polymorphisms and glycosylation.
Tharmalingam
Tharmala
T
National Institute for Bioprocessing Research and Training, Dublin-Oxford Glycobiology Laboratory, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland.
Mariño
Karina
K
Rudd
Pauline M
PM
D015415:Biomarkers / Q000097:blood; D004194:Disease / Q000235:genetics*; D004777:Environment; D054794:Glycomics / Q000379:methods*; D006801:Humans; D011134:Polysaccharides / Q000097:blood* / Q000235:genetics*; D012371:Robotics
D015415:Biomarkers; D011134:Polysaccharides