[Effect of bone morphogenetic protein-7 on aristolochic acid induced renal tubular epithelial cells transdifferentiation].
OBJECTIVE
To observe the effect of bone morphogenetic protein-7 (BMP-7) on aristolchic acid induced renal tubular epithelial cell trans-differentiation to look for new therapeutic approach for aristolchic acid nephropathy (AAN).


METHODS
In vitro cultured human proximal renal tubular epithelial cell line HK-2 cells were treated with different concentrations of BMP-7 (75 ng/mL, 150 ng/mL and 300 ng/mL) after trans-differentiation of the cells was induced by AA (10 microg/mL). Levels of alpha-SMA mRNA and protein expressions were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting respectively.


RESULTS
BMP-7 reversed the AA inducing alpha-SMA expressions in HK-2 cells in a dose-dependent manner.


CONCLUSIONS
BMP-7 can inhibit the trans-differentiation of human renal tubular epithelial cell induced by AA, thereby might be a new potential drug for AAN prevention and treatment.
Xu
Yong-zhi
YZ
Institute of Nephrology, Afiliated Hospital of Guangdong Medical College.
Huang
Hao
H
Liu
Hua-feng
HF
D000199:Actins / Q000378:metabolism; D034341:Aristolochic Acids / Q000009:adverse effects*; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology*; D002460:Cell Line; D054338:Cell Transdifferentiation / Q000187:drug effects*; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000166:cytology
C541116:ACTA2 protein, human; D000199:Actins; D034341:Aristolochic Acids; D055419:Bone Morphogenetic Protein 7; C000228:aristolochic acid I