N-cadherin-dependent neuron-neuron interaction is required for the maintenance of activity-induced dendrite growth.
Formation of neural circuits depends on stable contacts between neuronal processes, mediated by interaction of cell adhesion molecules, including N-cadherin. In the present study, we found that activity-dependent dendrite arborization specifically requires N-cadherin-mediated extracellular neuron-neuron interaction, because the enhancement did not occur for neurons cultured in isolation or plated on an astrocyte monolayer and was abolished by a recombinant soluble N-cadherin ectodomain. Furthermore, depolarization elevated the level of membrane-associated cadherin/catenin complexes and surface N-cadherin. Importantly, surface N-cadherin elevation is specifically required for the maintenance of nascent dendrite arbors. Through loss- and gain-of-function approaches, we showed that N-cadherin-mediated dendrite growth requires association of the cadherin/catenin complex with the actin cytoskeleton. In summary, these results identify a previously unexplored and specific function for activity-induced, N-cadherin-mediated neuron-neuron contacts in the maintenance of dendrite arbors.
Tan
Zhu-Jun
ZJ
Institute of Neuroscience and State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Shanghai 200031, China. yuxiang@ion.ac.cn
Peng
Yun
Y
Song
He-Ling
HL
Zheng
Jing-Jing
JJ
Yu
Xiang
X
D000199:Actins / Q000378:metabolism; D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D051177:Catenins / Q000378:metabolism; D002448:Cell Adhesion; D002450:Cell Communication; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley
D000199:Actins; D015820:Cadherins; D051177:Catenins
J Biol Chem. 1995 May 5;270(18):10999-1003
7738042
Neuron. 2009 Feb 12;61(3):340-50
19217372
Trends Cell Biol. 2005 Feb;15(2):64-7
15695092
Nat Rev Mol Cell Biol. 2007 Jan;8(1):37-48
17183359
J Cell Biol. 1990 Apr;110(4):1253-60
2324197
Nat Neurosci. 2003 Nov;6(11):1169-77
14528308
Neuron. 2002 Jul 3;35(1):91-105
12123611
J Comp Neurol. 2006 Mar 20;495(3):324-35
16440298
Cell. 2005 Dec 2;123(5):889-901
16325582
Neuron. 2002 Jul 3;35(1):77-89
12123610
Traffic. 2005 Dec;6(12):1142-56
16262725
Neuron. 2007 Jun 7;54(5):771-85
17553425
Annu Rev Neurosci. 2007;30:399-423
17378766
Nat Rev Neurosci. 2007 Mar;8(3):206-20
17299456
J Neurosci. 2006 Mar 29;26(13):3594-603
16571768
Annu Rev Neurosci. 2007;30:425-50
17417940
Brain Res. 1981 Aug 10;218(1-2):299-318
7272738
Neuron. 1991 Feb;6(2):247-58
1993122
Curr Opin Neurobiol. 2008 Feb;18(1):53-9
18508258
Biochem Soc Trans. 2008 Apr;36(Pt 2):149-55
18363555
Annu Rev Cell Dev Biol. 2007;23:613-43
17506699
J Neurocytol. 2000 Mar;29(3):215-23
11428051
Neuropharmacology. 2004 Oct;47(5):779-86
15458849
Annu Rev Cell Dev Biol. 2008;24:597-620
18837673
J Physiol. 2008 Mar 15;586(6):1509-17
18202093
Neuron. 2002 Jun 13;34(6):999-1010
12086646
Acta Neurobiol Exp (Wars). 2008;68(2):264-88
18511961
Cell. 2005 Dec 2;123(5):903-15
16325583
Trends Neurosci. 1990 Aug;13(8):312-8
1699312
Hippocampus. 2008;18(4):349-63
18064706
J Cell Biol. 1994 Oct;127(1):235-45
7929566
Science. 2004 Sep 24;305(5692):1972-5
15448273
J Neurobiol. 2005 Jul;64(1):4-10
15884010
Synapse. 1988;2(1):69-78
2458630
J Neurosci. 2004 Oct 13;24(41):9027-34
15483121
J Neurosci. 2006 Mar 29;26(13):3604-14
16571769
Trends Cell Biol. 2006 Sep;16(9):453-60
16893648
Nat Neurosci. 2004 Mar;7(3):254-60
14758365
J Neurosci. 1998 Sep 1;18(17):6892-904
9712659