Multiphysics simulation of a microfluidic perfusion chamber for brain slice physiology.
Understanding and optimizing fluid flows through in vitro microfluidic perfusion systems is essential in mimicking in vivo conditions for biological research. In a previous study a microfluidic brain slice device (microBSD) was developed for microscale electrophysiology investigations. The device consisted of a standard perfusion chamber bonded to a polydimethylsiloxane (PDMS) microchannel substrate. Our objective in this study is to characterize the flows through the microBSD by using multiphysics simulations of injections into a pourous matrix to identify optimal spacing of ports. Three-dimensional computational fluid dynamic (CFD) simulations are performed with CFD-ACE + software to model, simulate, and assess the transport of soluble factors through the perfusion bath, the microchannels, and a material that mimics the porosity, permeability and tortuosity of brain tissue. Additionally, experimental soluble factor transport through a brain slice is predicted by and compared to simulated fluid flow in a volume that represents a porous matrix material. The computational results are validated with fluorescent dye experiments.
Caicedo
Hector H
HH
Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, USA.
Hernandez
Maximiliano
M
Fall
Christopher P
CP
Eddington
David T
DT
D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D051379:Mice; D046210:Microfluidic Analytical Techniques; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D010477:Perfusion / Q000295:instrumentation*; D010825:Physics; D016062:Porosity
D004129:Dimethylpolysiloxanes; C013830:baysilon
J Neurosci. 2005 Aug 31;25(35):8027-36
16135760
Lab Chip. 2007 Nov;7(11):1475-8
17960274
Lab Chip. 2010 Jan 7;10(1):23-6
20024045
Am J Physiol. 1997 Nov;273(5):R1810-21
9374827
Trends Neurosci. 1988 Nov;11(11):484-9
2469173
J Neurosci Methods. 1981 Dec;4(4):329-42
7033675
J Control Release. 2006 Apr 10;111(3):252-62
16476500
Lab Chip. 2007 Jul;7(7):842-9
17594002
Brain Res Bull. 1983 Jun;10(6):853-7
6311362
Brain Res. 2006 May 9;1088(1):73-82
16626659
Lab Chip. 2004 Apr;4(2):91-7
15052346
Lab Chip. 2008 Jul;8(7):1048-55
18584078
Synapse. 1989;3(4):331-8
2740992
Biomed Microdevices. 2007 Feb;9(1):7-13
17091392
J Theor Biol. 2008 Jan 7;250(1):125-38
17950757
J Neurosci Methods. 1980 Aug;2(4):323-62
6106092
J Neurosci Methods. 1979 Dec;1(4):323-5
544974