title: [Gene characteristic of VP1 region of poliovirus isolates from acute flaccid paralysis in Hebei Province].
abstract: OBJECTIVE
  In order to provide the evidence for polio free status, the VP1 gene characteristic analysis of poliovirus isolated from acute flaccid paralysis (AFP) cases and close contacts were conducted in Hebei Province during 2007-2008.
  
  
  METHODS
  Virus isolation and identification were conducted according to the 4th edition of WHO polio laboratory manual. The sequence of VP1 region were amplified and sequenced. The phylogenetic trees based on VP1 region were constructed.
  
  
  RESULTS
  The total number of poliovirus isolates were 42. Most isolates were type II and III. No wild poliovirus and vaccine derived poliovirus (VDPVs) was detected.
  
  
  CONCLUSIONS
  Gene characteristic of VP1 region of poliovirus can be used to identify the polio virus transmission and guide the work of immunization program.
authors.affiliation: Hebei Provincial Center for Disease Control and Prevention, Shijiazhuang 050021, Hebei, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Yan
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002681:China / Q000453:epidemiology; D006801:Humans; D051379:Mice; D010264:Paraplegia / Q000453:epidemiology / Q000821:virology*; D010802:Phylogeny; D017955:Poliovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins; C442540:VP1 protein, Poliovirus