Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O12.
The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O12. Its structure was studied by sugar analysis using GLC of the alditol acetates and (S)-2-octyl glycosides, methylation analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy, including 2D (1)H-(1)H COSY, TOCSY, ROESY, (1)H-(13)C HSQC, and HMBC experiments. It was found that the polymer is a neutral heteropolysaccharide and has a branched heptasaccharide repeating unit with the following structure:
Parkhomchuk
Angelina A
AA
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation. lina_chuk@mail.ru
Kocharova
Nina A
NA
Białczak-Kokot
Magdalena
M
Shashkov
Alexander S
AS
Chizhov
Alexander O
AO
Knirel
Yuriy A
YA
Rozalski
Antoni
A
D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011532:Providencia / Q000737:chemistry*
D019081:O Antigens