title: [Relationship between T704C polymorphism of angiotensinogen gene and cerebral hemorrhage in Han people in Changsha].
abstract: OBJECTIVE
  To investigate the relationship between T704C polymorphism of angiotensinogen (AGT) gene and cerebral hemorrhage and its impact on the levels of blood pressure in Han people in Changsha.
  
  
  METHODS
  A total of 273 cerebral hemorrhage patients (the cerebral hemorrhage group) and 140 normal controls (the control group) were collected from Jan. 2005 to Jan. 2009. DNA was extracted from their peripheral blood samples. The polymorphism of AGT-T704C was analyzed by SNaPshot and direct DNA sequencing. The possible risk factors of cerebral hemorrhage were investigated at the same time. Each group was divided into 2 subgroups (a high blood pressure subgroup and a normal blood pressure subgroup) according to whether they had essential hypertension. Logistic regression analysis was used to detect the relationship between cerebral hemorrhage and all its possible risk factors and AGT-T704C polymorphism.
  
  
  RESULTS
  The drinking history, coronary heart disease history, essential hypertension history, and blood levels of lipids were shown significant difference between the cerebral hemorrhage group and the control group (P<0.05). Logistic regression analysis showed that hypertension history, systolic blood pressure level, and high density lipoprotein cholesterol level were independent risk factors for cerebral hemorrhage in Han people in Changsha. The genotype C/C, C/T, and T/T frequencies of AGT-T704C polymorphism in the cerebral hemorrhage group and the control group were 0.692, 0.279, 0.029 and 0.629, 0.350, 0.021, respectively. The allele C and T frequencies of AGT-T704C polymorphism in the 2 groups were 0.832, 0.168 and 0.804, 0.196, respectively. The frequencies of all the genotypes and alleles had no significant difference between the 2 groups and their subgroups (P>0.05).
  
  
  CONCLUSIONS
  The polymorphism of AGT-T704C may not be associated with cerebral hemorrhage and not related to the levels of lipids and blood pressure in Han people in Changsha. Hypertension history, systolic blood pressure level, and high density lipoprotein cholesterol level are the main risk factors of cerebral hemorrhage in Han people in Changsha.
authors.affiliation: Department of Geriatrics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
authors.forename: Mingming
authors.initials: M
authors.lastname: Ma
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Hu
authors.forename: Baoqiong
authors.initials: B
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
authors.forename: Yunhai
authors.initials: Y
authors.lastname: Liu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Xu
authors.forename: Hongxing
authors.initials: H
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000808:Angiotensinogen / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D002543:Cerebral Hemorrhage / Q000235:genetics*; D002681:China / Q000208:ethnology; D005260:Female; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000150:complications; D008075:Lipoproteins, HDL / Q000097:blood; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012307:Risk Factors; D055815:Young Adult
chemical_list: D008075:Lipoproteins, HDL; D000808:Angiotensinogen