title: The immunoexpression of FSH-R in the ductuli efferentes and the epididymis of men and rat: effect of FSH on the morphology and steroidogenic activity of rat epididymal epithelial cells in vitro.
abstract: The Sertoli cells were regarded as the only target for FSH in male reproductive system. The expression of FSH receptor (FSH-R) was detected also in epithelial cells of the caput epididymis of rat and monkey. We showed in the immunohistochemistry study the expression of FSH-R in rat and human ductuli efferentes and the caput, corpus, and cauda epididymis, moreover, by Western blot analysis in the caput and cauda epididymis of rat. Additionally, we presented that the morphology of rat epididymal epithelial cells in vitro was affected by FSH, and FSH stimulation resulted in the increase of 17beta-estradiol synthesis by rat caput epididymal cells in dose-depended manner. In conclusion, the identification of FSH receptors in human and rat epididymides supports our results that the epididymis is a target organ not only for LH but additionally for FSH. On the basis of the results we showed for the first time that morphology of epididymal epithelial cells and epididymal steroidogenesis can be regulated by FSH.
authors.affiliation: Department of Histology and Embryology, Pomeranian Medical University, 72 Powstańców Wlkp. Street, 70-111 Szczecin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Swider-Al-Amawi
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kolasa
authors.forename: Andrzej
authors.initials: A
authors.lastname: Sikorski
authors.forename: Mariola
authors.initials: M
authors.lastname: Marchlewicz
authors.forename: Irena
authors.initials: I
authors.lastname: Baranowska-Bosiacka
authors.forename: Barbara
authors.initials: B
authors.lastname: Wiszniewska
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004822:Epididymis / Q000378:metabolism*; D004958:Estradiol; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011962:Receptors, FSH / Q000378:metabolism*; D012670:Seminiferous Epithelium / Q000166:cytology / Q000378:metabolism; D012671:Seminiferous Tubules / Q000378:metabolism*; D018709:Statistics, Nonparametric
chemical_list: D011962:Receptors, FSH; D004958:Estradiol; D005640:Follicle Stimulating Hormone
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