Effects of surfactant and salt species in reverse micellar forward extraction efficiency of isoflavones with enriched protein from soy flour.
Suitability of reverse micelles of anionic surfactant sodium bis(2-ethyl hexyl) sulfosuccinate (AOT) and sodium dodecyl sulfate (SDS), cationic surfactant hexadecyl trimethyl ammonium bromide (CTAB) and nonionic surfactant polyoxyethylene p-t-octylphenol (TritonX-100) in organic solvent isooctane for extraction of soy isoflavone-enriching proteins was investigated. The results showed that the order of combined isoflavone contents was SDS>CTAB>Triton X-100>AOT, while the order of protein recovery was SDS>AOT>TritonX-100>CTAB. As compared with ACN-HCl extraction, the total amount of isoflavones was lower than reverse micellar extraction. Ion strength was one of the important conditions to control extraction of isoflavone-enriching proteins with AOT reversed micelles. For the six salt systems, KNO(3), KCl, MgCl(2), CaCl(2), NaCl, and Na(2)SO(4), extracted fraction of isoflavone-enriching proteins was measured. Salt solutions greatly influenced the extraction efficiency of isoflavones in an order of KNO(3)>MgCl(2)>CaCl(2)>KCl>NaCl>Na(2)SO(4), while protein in an order of MgCl(2)>CaCl(2)>NaCl>KNO(3)>Na(2)SO(4)>KCl.
Zhao
Xiaoyan
X
Institute Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, China. zhaoxiaoy@yahoo.cn
Wei
Zhiyi
Z
Du
Fangling
F
Zhu
Junqing
J
D005591:Chemical Fractionation / Q000379:methods*; D005433:Flour / Q000032:analysis*; D007529:Isoflavones / Q000032:analysis / Q000302:isolation & purification*; D008823:Micelles; D009994:Osmolar Concentration; D012492:Salts / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
D007529:Isoflavones; D008823:Micelles; D012492:Salts; D013501:Surface-Active Agents