Guidelines for tunneling in enzymes.
Here we extend the engineering descriptions of simple, single-electron-tunneling chains common in oxidoreductases to quantify sequential oxidation-reduction rates of two-or-more electron cofactors and substrates. We identify when nicotinamides may be vulnerable to radical mediated oxidation-reduction and merge electron-tunneling expressions with the chemical rate expressions of Eyring. The work provides guidelines for the construction of new artificial oxidoreductases inspired by Nature but adopting independent design and redox engineering.
Moser
Christopher C
CC
Dept. Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA. moserc@mail.med.upenn.edu
Anderson
J L Ross
JL
Dutton
P Leslie
PL
D004579:Electron Transport; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry*; D010788:Photosynthesis; D015202:Protein Engineering
D010088:Oxidoreductases
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