Multimodal nonlinear optical imaging of atherosclerotic plaque development in myocardial infarction-prone rabbits.
Label-free imaging of bulk arterial tissue is demonstrated using a multimodal nonlinear optical microscope based on a photonic crystal fiber and a single femtosecond oscillator operating at 800 nm. Colocalized imaging of extracellular elastin fibers, fibrillar collagen, and lipid-rich structures within aortic tissue obtained from atherosclerosis-prone myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits is demonstrated through two-photon excited fluorescence, second harmonic generation, and coherent anti-Stokes Raman scattering, respectively. These images are shown to differentiate healthy arterial wall, early atherosclerotic lesions, and advanced plaques. Clear pathological changes are observed in the extracellular matrix of the arterial wall and correlated with progression of atherosclerotic disease as represented by the age of the WHHLMI rabbits.
Ko
Alex C T
AC
National Research Council Canada, Institute for Biodiagnostics, 435 Ellice Avenue, Winnipeg, Manitoba, Canada R3B 1Y6. alex.ko@nrc-cnrc.gc.ca
Ridsdale
Andrew
A
Smith
Michael S D
MS
Mostaço-Guidolin
Leila B
LB
Hewko
Mark D
MD
Pegoraro
Adrian F
AF
Kohlenberg
Elicia K
EK
Schattka
Bernie
B
Shiomi
Masashi
M
Stolow
Albert
A
Sowa
Michael G
MG
D000818:Animals; D003324:Coronary Artery Disease / Q000150:complications* / Q000473:pathology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D018613:Microscopy, Confocal / Q000295:instrumentation*; D009203:Myocardial Infarction / Q000209:etiology* / Q000473:pathology*; D017711:Nonlinear Dynamics; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity