The need for imaging methods in bioengineering of three-dimensional cell cultures.
The analysis of live cells and tissue is gaining increasing importance in research and development. A number of demands are thus being made on the cultivation and analysis of cell response as well. For certain research approaches, in vitro three-dimensional (3D) cultivation best mimics natural conditions. Nevertheless, the imaging of such 3D environments is still a problem. To understand cell function in 3D, a number of in vivo imaging methods have been developed. This article introduces this field and proposes some interesting and promising imaging techniques for this approach.
Freimark
Denise
D
Institute of Biopharmaceutical Technology, University of Applied Sciences Giessen-Friedberg, Giessen - Germany. denixe.freimark@tg.fh-giessen.de
Ehlicke
Franziska
F
Czermak
Peter
P
D057005:Bioengineering; D018929:Cell Culture Techniques / Q000379:methods*; D021621:Imaging, Three-Dimensional; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods