Transverse flow imaging based on photoacoustic Doppler bandwidth broadening.
We propose a new method to measure transverse flow velocity based on photoacoustic Doppler bandwidth broadening, which is determined by the geometry of the probe-beam and the velocity of the transverse flow. By exploiting pulsed laser excitation and raster motor scanning, three-dimensional structure and flow velocity can be imaged simultaneously. In addition, the flow direction can be determined with bidirectional scanning. In a flowing suspension of red-dyed microspheres (diameter: 6 microm), transverse flow speeds ranging from 0 to 2.5 mms as well as flow direction were measured. A cross-sectional flow image was also obtained with the tube laid in a zigzag pattern.
Yao
Junjie
J
Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, Campus Box 1097, One Brookings Drive, St. Louis, Missouri 63130, USA.
Wang
Lihong V
LV
D001783:Blood Flow Velocity / Q000502:physiology*; D017076:Computer-Aided Design; D054459:Elasticity Imaging Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017078:Laser-Doppler Flowmetry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
Med Phys. 2008 Dec;35(12):5758-67
19175133
Cytometry A. 2008 Oct;73(10):884-94
18677768
Opt Lett. 2000 Jan 15;25(2):114-6
18059800
J Biomed Opt. 2007 Sep-Oct;12(5):051503
17994867
Phys Med Biol. 2003 Aug 7;48(15):2405-18
12953906
Ann Thorac Surg. 2003 Jan;75(1):204-9
12537217
IEEE Trans Ultrason Ferroelectr Freq Control. 1998;45(3):574-80
18244209
J Physiol. 2001 Jun 15;533(Pt 3):773-85
11410634
Nat Biotechnol. 2003 Jul;21(7):803-6
12808463
Opt Lett. 2002 Mar 15;27(6):409-11
18007817
Opt Lett. 2009 Mar 1;34(5):671-3
19252588
Opt Lett. 2005 Dec 15;30(24):3341-3
16389825
Opt Lett. 2006 Dec 15;31(24):3623-5
17130924
Cancer Res. 1989 Dec 1;49(23):6449-65
2684393
Phys Rev Lett. 2007 Nov 2;99(18):184501
17995411
Nat Biotechnol. 2006 Jul;24(7):848-51
16823374
Opt Lett. 2000 Sep 15;25(18):1358-60
18066216
Crit Rev Biomed Eng. 1993;21(5):399-483
8269708
Opt Lett. 2008 May 1;33(9):929-31
18451942
J Invest Dermatol. 1977 Sep;69(3):306-9
894068
IEEE Trans Biomed Eng. 1987 Oct;34(10):779-89
3679261