Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells.
We present the light scattering of individual Plasmodium falciparum-parasitized human red blood cells (Pf-RBCs), and demonstrate progressive alterations to the scattering signal arising from the development of malaria-inducing parasites. By selectively imaging the electric fields using quantitative phase microscopy and a Fourier transform light scattering technique, we calculate the light scattering maps of individual Pf-RBCs. We show that the onset and progression of pathological states of the Pf-RBCs can be clearly identified by the static scattering maps. Progressive changes to the biophysical properties of the Pf-RBC membrane are captured from dynamic light scattering.
Park
YongKeun
Y
Massachusetts Institute of Technology, G. R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. ykpark@mit.edu
Diez-Silva
Monica
M
Fu
Dan
D
Popescu
Gabriel
G
Choi
Wonshik
W
Barman
Ishan
I
Suresh
Subra
S
Feld
Michael S
MS
D004912:Erythrocytes / Q000469:parasitology* / Q000473:pathology*; D006801:Humans; D008027:Light; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D010963:Plasmodium falciparum / Q000472:pathogenicity / Q000502:physiology*; D012016:Reference Values; D012542:Scattering, Radiation
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