title: Rhizobium borbori sp. nov., aniline-degrading bacteria isolated from activated sludge.
abstract: Three aniline-degrading bacteria, strains DN316(T), DN316-1 and DN365, were isolated from activated sludge. According to 16S rRNA gene sequence-based phylogenetic analysis, the isolates belonged to the genus Rhizobium, with Rhizobium ( = Agrobacterium) radiobacter LMG 140(T) as the closest relative, with 96.5 % sequence similarity. Phylogenetic analysis of the representative strain DN316(T) using sequences of the glnA, thrC and recA genes and the 16S-23S intergenic spacer region confirmed the phylogenetic arrangement obtained from analysis of the 16S rRNA gene. DNA-DNA relatedness between DN316(T) and R. radiobacter LMG 140(T) was 43.7 %, clearly indicating that the representative strain DN316(T) represents a novel species. Phenotypic and biochemical characterization of the isolates and insertion sequence-PCR fingerprinting patterns showed several distinctive features that differentiated them from closely related species. The major components of the cellular fatty acids were C(18 : 1)ω7c (57.10 %), C(16 : 0) (11.31 %) and C(19 : 0) cyclo ω8c (10.13 %). Based on our taxonomic analysis, the three isolates from activated sludge represent a novel species of the genus Rhizobium, for which the name Rhizobium borbori sp. nov. is proposed. The type strain is DN316(T) ( = CICC 10378(T)  = LMG 23925(T)).
authors.affiliation: Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, PR China.|Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
authors.forename: Guo Xia
authors.initials: GX
authors.lastname: Zhang
authors.affiliation: Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
authors.forename: Sui Zhou
authors.initials: SZ
authors.lastname: Ren
authors.affiliation: Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
authors.forename: Mei Ying
authors.initials: MY
authors.lastname: Xu
authors.affiliation: Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
authors.forename: Guo Qu
authors.initials: GQ
authors.lastname: Zeng
authors.affiliation: Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
authors.forename: Hui Dong
authors.initials: HD
authors.lastname: Luo
authors.affiliation: Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
authors.forename: Jin Lin
authors.initials: JL
authors.lastname: Chen
authors.affiliation: Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Zhi Yuan
authors.initials: ZY
authors.lastname: Tan
authors.affiliation: Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
authors.forename: Guo Ping
authors.initials: GP
authors.lastname: Sun
mesh_terms: D000814:Aniline Compounds / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D005974:Glutamate-Ammonia Ligase; D008969:Molecular Sequence Data; D058889:Molecular Typing; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
chemical_list: D000814:Aniline Compounds; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D011938:Rec A Recombinases; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase; C023650:aniline