title: Cardiospheres and tissue engineering for myocardial regeneration: potential for clinical application.
abstract: Tissue engineering is an increasingly expanding area of research in the cardiovascular field that involves engineering, chemistry, biology and medicine. Cardiac tissue engineering (CTE) aims to regenerate myocardial damage by combining cells, matrix, biological active molecules and physiological stimuli. The rationale behind CTE applications is that in order to regenerate the ventricular wall after a myocardial infarction it is necessary to combine procedures that regenerate both cardiomyocytes and the extracellular matrix. The application of (stem) cells together with a matrix could represent an environment protected from the inflammatory and pro-apoptotic signals, a stemness/survival reservoir slowly releasing cells and factors promoting tissue regeneration and angiogenesis. This review will focus on the applications and advantages that CTE application could offer compared to conventional cell therapy.
authors.affiliation: Department of Experimental Medicine, Cenci-Bolognetti Foundation, Pasteur Institute, University of Rome Sapienza, Rome, Italy. R.Gaetani@umcutrecht.nl
authors.forename: Roberto
authors.initials: R
authors.lastname: Gaetani
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Rizzitelli
authors.forename: Isotta
authors.initials: I
authors.lastname: Chimenti
authors.forename: Lucio
authors.initials: L
authors.lastname: Barile
authors.forename: Elvira
authors.initials: E
authors.lastname: Forte
authors.forename: Vittoria
authors.initials: V
authors.lastname: Ionta
authors.forename: Francesco
authors.initials: F
authors.lastname: Angelini
authors.forename: Joost P G
authors.initials: JP
authors.lastname: Sluijter
authors.forename: Andrea
authors.initials: A
authors.lastname: Barbetta
authors.forename: Elisa
authors.initials: E
authors.lastname: Messina
authors.forename: Giacomo
authors.initials: G
authors.lastname: Frati
mesh_terms: D006801:Humans; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism*; D012038:Regeneration / Q000502:physiology*; D018874:Spheroids, Cellular / Q000166:cytology* / Q000378:metabolism; D033581:Stem Cell Transplantation; D023822:Tissue Engineering / Q000379:methods*
references.citation: J Am Coll Cardiol. 2005 Aug 16;46(4):714-9
references.pmid: 16098441
references.citation: J Am Coll Cardiol. 2009 Oct 20;54(17):1619-26
references.pmid: 19833262
references.citation: Cell Transplant. 2002;11(7):621-30
references.pmid: 12518889
references.citation: Circulation. 2007 Feb 20;115(7):896-908
references.pmid: 17283259
references.citation: Nat Med. 2006 Apr;12(4):452-8
references.pmid: 16582915
references.citation: Nat Protoc. 2009;4(2):232-43
references.pmid: 19197267
references.citation: Proc Natl Acad Sci U S A. 2006 May 23;103(21):8155-60
references.pmid: 16698918
references.citation: Cardiovasc Hematol Agents Med Chem. 2009 Apr;7(2):91-107
references.pmid: 19355872
references.citation: Cell Transplant. 2009;18(3):297-304
references.pmid: 19558778
references.citation: Neth Heart J. 2008 May;16(5):163-9
references.pmid: 18566670
references.citation: Circulation. 2008 Mar 18;117(11):1388-96
references.pmid: 18316487
references.citation: J Clin Invest. 2006 Jan;116(1):237-48
references.pmid: 16357943
references.citation: Cell Transplant. 2007;16(9):927-34
references.pmid: 18293891
references.citation: J Thorac Cardiovasc Surg. 2009 Jan;137(1):180-7
references.pmid: 19154923
references.citation: Circ Res. 2004 Oct 29;95(9):911-21
references.pmid: 15472116
references.citation: J Mol Cell Cardiol. 2008 Oct;45(4):567-81
references.pmid: 18466917
references.citation: Nat Clin Pract Cardiovasc Med. 2009 Mar;6(3):240-9
references.pmid: 19234502
references.citation: Circulation. 2009 Sep 8;120(10):876-87
references.pmid: 19704095
references.citation: J Mol Cell Cardiol. 2006 Nov;41(5):876-84
references.pmid: 16973174
references.citation: Circ Res. 2010 Mar 19;106(5):971-80
references.pmid: 20110532
references.citation: Tissue Eng. 2005 May-Jun;11(5-6):803-13
references.pmid: 15998220
references.citation: Biomacromolecules. 2009 Dec 14;10(12):3188-92
references.pmid: 19891500
references.citation: J Am Coll Cardiol. 2004 Aug 4;44(3):654-60
references.pmid: 15358036
references.citation: Cardiovasc Res. 2007 Jul 1;75(1):178-85
references.pmid: 17481597
references.citation: J Mol Cell Cardiol. 2001 May;33(5):907-21
references.pmid: 11343414
references.citation: Tissue Eng. 2003 Jun;9(3):517-23
references.pmid: 12857419
references.citation: Tissue Eng. 2005 Nov-Dec;11(11-12):1860-6
references.pmid: 16411832
references.citation: Tissue Eng. 2007 Nov;13(11):2681-7
references.pmid: 17691866
references.citation: Circulation. 2006 Jul 4;114(1 Suppl):I167-73
references.pmid: 16820568
references.citation: Circulation. 2005 Aug 30;112(9 Suppl):I150-6
references.pmid: 16159808
references.citation: Biomaterials. 2009 Oct;30(29):5409-16
references.pmid: 19608268