Quercetin attenuates oxidative damage induced by treatment of embryonic chicken spermatogonial cells with 4-nitro-3-phenylphenol in diesel exhaust particles.
Quercetin, an antioxidant flavonoid, is considered beneficial for human and animal health. In this study, the protective effect of quercetin on oxidative damage to testicular cells was studied in embryonic chickens after treatment with 4-nitro-3-phenylphenol (PNMPP) derived from diesel exhaust particles. Testicular cells were challenged with PNMPP (10(-8)-10(-6) M) alone and in combination with quercetin for 48 h. The results showed that quercetin manifested no deleterious effect on spermatogonial cells up to 1.0 microg/ml. Exposure to PNMPP (10(-6) M) induced condensed nuclei and vacuolated cytoplasm and reductions in testicular cell viability and spermatogonial cell numbers (p<0.05). It also induced lipid peroxidation by an elevation of thiobarbituric acid reactive substances and decreased glutathione peroxidase activity and superoxide dismutase activity (p<0.05). Simultaneous supplementation with quercetin restored these parameters to the same levels as in the control. These data indicate that quercetin protects spermatogonial cells from oxidative damage in embryonic chickens intoxicated with PNMPP.
Mi
Yuling
Y
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Zhang
Caiqiao
C
Li
ChunMei
C
Taneda
Shinji
S
Watanabe
Gen
G
Suzuki
Akira K
AK
Taya
Kazuyoshi
K
D000818:Animals; D001713:Biphenyl Compounds / Q000633:toxicity*; D002452:Cell Count; D002645:Chickens; D018920:Coculture Techniques; D004625:Embryo, Nonmammalian; D004785:Environmental Pollutants / Q000633:toxicity; D016166:Free Radical Scavengers / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009596:Nitrophenols / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology*; D012098:Reproduction / Q000187:drug effects; D013093:Spermatogonia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D001335:Vehicle Emissions
C494060:4-nitro-3-phenylphenol; D001713:Biphenyl Compounds; D004785:Environmental Pollutants; D016166:Free Radical Scavengers; D009596:Nitrophenols; D001335:Vehicle Emissions; D008315:Malondialdehyde; D011794:Quercetin; D013482:Superoxide Dismutase; D005978:Glutathione