Biomimetic hybrid scaffolds for engineering human tooth-ligament interfaces.
A major clinical challenge in the reconstruction of large oral and craniofacial defects is the neogenesis of osseous and ligamentous interfacial structures. Currently, oral regenerative medicine strategies are unpredictable for repair of tooth-supporting tissues destroyed as a consequence of trauma, chronic infection or surgical resection. Here, we demonstrate multi-scale computational design and fabrication of composite hybrid polymeric scaffolds for targeted cell transplantation of genetically modified human cells for the formation of human tooth dentin-ligament-bone complexes in vivo. The newly-formed tissues demonstrate the interfacial generation of parallel- and obliquely-oriented fibers that grow and traverse within the polycaprolactone (PCL)-poly(glycolic acid) (PGA) designed constructs forming tooth cementum-like tissue, ligament, and bone structures. This approach offers potential for the clinical implementation of customized periodontal scaffolds that may enable regeneration of multi-tissue interfaces required for oral, dental and craniofacial engineering applications.
Park
Chan Ho
CH
Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109-1078, USA.
Rios
Hector F
HF
Jin
Qiming
Q
Bland
Megan E
ME
Flanagan
Colleen L
CL
Hollister
Scott J
SJ
Giannobile
William V
WV
D006801:Humans; D008022:Ligaments / Q000502:physiology*; D018716:Molecular Mimicry; D023822:Tissue Engineering; D014057:Tomography, X-Ray Computed; D014070:Tooth / Q000502:physiology*
J Biomech. 2009 Aug 25;42(12):1961-6
19501361
J Clin Periodontol. 1998 Feb;25(2):134-44
9495612
Cell. 2007 Aug 24;130(4):601-10
17719539
Nat Biotechnol. 2003 Sep;21(9):1025-32
12949568
J Dent Res. 2000 Dec;79(12):1962-8
11201046
J Cell Biol. 2009 Feb 23;184(4):481-90
19221195
Adv Mater. 2009 Sep 4;21(32-33):3330-42
20882500
Science. 2009 Jun 26;324(5935):1673-7
19556500
Nat Rev Mol Cell Biol. 2009 Aug;10(8):538-49
19603038
Science. 2005 Nov 18;310(5751):1139-43
16293750
Med Res Rev. 2009 May;29(3):472-513
19260070
J Dent Res. 2004 Oct;83(10):751-6
15381713
Lancet. 1999 Jul;354 Suppl 1:SI32-4
10437854
Biomaterials. 2003 Jan;24(1):181-94
12417192
Curr Stem Cell Res Ther. 2010 Jun;5(2):168-74
19941449
Nat Mater. 2009 Jun;8(6):457-70
19458646
Periodontol 2000. 2006;40:11-28
16398683
Lancet. 2005 Nov 19;366(9499):1809-20
16298220
J Periodontal Res. 2006 Aug;41(4):311-5
16827725
Dent Clin North Am. 2006 Apr;50(2):245-63, ix
16530061
Nat Mater. 2009 Jan;8(1):15-23
19096389
Bone. 2009 Dec;45(6):1104-16
19651256
J Bone Miner Res. 2009 Sep;24(9):1507-11
19653806
Science. 1993 May 14;260(5110):920-6
8493529
J Dent Res. 2009 Sep;88(9):792-806
19767575
J Biomed Mater Res A. 2008 Jul;86(1):1-12
18442111
Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13661-5
9391082
Proc Natl Acad Sci U S A. 2008 Jun 10;105(23):7947-52
18541916
Nat Mater. 2005 Jul;4(7):518-24
16003400