title: Chlorella vulgaris aldehyde reductase is capable of functioning as ferric reductase and of driving the fenton reaction in the presence of free flavin.
abstract: The free flavin-dependent Fenton reaction was detected in cell-free extracts of Chlorella. The corresponding enzyme was purified to homogeneity, and its N-terminal sequence was highly homologous to those of aldo-keto reductase family enzymes. The purified enzyme displayed aldehyde reductase activity in the presence of NADPH. Additionally, it showed ferric reductase activity and drove the Fenton reaction in the presence of free FAD and NADH.
authors.affiliation: Department of Bioscience, Tokyo University of Agriculture, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Sato
authors.forename: Kouji
authors.initials: K
authors.lastname: Takeda
authors.forename: Rika
authors.initials: R
authors.lastname: Nishiyama
authors.forename: Kouichi
authors.initials: K
authors.lastname: Fusayama
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Arai
authors.forename: Takumi
authors.initials: T
authors.lastname: Sato
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Watanabe
authors.forename: Akira
authors.initials: A
authors.lastname: Abe
authors.forename: Junichi
authors.initials: J
authors.lastname: Nakagawa
authors.forename: Shinji
authors.initials: S
authors.lastname: Kawasaki
authors.forename: Youichi
authors.initials: Y
authors.lastname: Niimura
mesh_terms: D000429:Alcohol Oxidoreductases; D000449:Aldehyde Reductase / Q000235:genetics / Q000378:metabolism*; D000074408:Aldo-Keto Reductases; D001483:Base Sequence; D048408:Chlorella vulgaris / Q000235:genetics / Q000378:metabolism; D038181:FMN Reductase; D005415:Flavins / Q000235:genetics; D009243:NAD / Q000235:genetics / Q000378:metabolism; D009249:NADP / Q000235:genetics / Q000378:metabolism; D009930:Organic Chemicals
chemical_list: D005415:Flavins; D009930:Organic Chemicals; D009243:NAD; D009249:NADP; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase; D038181:FMN Reductase; C023903:ferric citrate iron reductase