Changes in the porcine peripheral blood mononuclear cell proteome induced by infection with highly virulent classical swine fever virus.
Leukopenia and immunosuppression are characteristic clinical manifestations of classical swine fever and peripheral blood mononuclear cells (PBMCs) are major targets of classical swine fever virus. To investigate proteomic expression changes in swine PBMCs during lethal CSFV infection, proteins of PBMCs from five lethally CSFV-infected pigs were resolved by two-dimensional electrophoresis followed by mass spectrometry. Quantitative intensity analysis revealed that 66 protein spots showed altered expression, 44 of which were identified as 34 unique proteins by MALDI-TOF-MS/MS. Cellular functions of these proteins included cytoskeletal, energy metabolism, protein translation and processing, antioxidative stress, heat shock and blood clotting. Western blot analysis confirmed the upregulation of annexin A1 and downregulation of cofilin. Identification of these changed levels of expression provides an understanding at the molecular level of the response of in vivo target cells to CSFV infection and of the pathogenic mechanisms of leukopenia and immunosuppression induced by the virus.
Sun
Jinfu
J
College of Science, Northeastern University, Shenyang, People's Republic of China.
Shi
Zixue
Z
Guo
Huancheng
H
Tu
Changchun
C
D051339:Actin Depolymerizing Factors / Q000235:genetics / Q000378:metabolism; D000818:Animals; D017305:Annexin A1 / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D006691:Classical Swine Fever / Q000097:blood* / Q000235:genetics / Q000276:immunology* / Q000821:virology; D006692:Classical Swine Fever Virus / Q000472:pathogenicity*; D017931:DNA Primers / Q000235:genetics; D007108:Immune Tolerance; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D007970:Leukopenia / Q000209:etiology; D020543:Proteome / Q000302:isolation & purification; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D034421:Sus scrofa; D013552:Swine; D053719:Tandem Mass Spectrometry
D051339:Actin Depolymerizing Factors; D017305:Annexin A1; D017931:DNA Primers; D020543:Proteome; D012333:RNA, Messenger; D012367:RNA, Viral