[Dot blot for the diagnosis of porcine cysticercosis].
The Taenia solium larva causes cysticercosis in pigs, as well as economic losses for farmers and taeniosis in humans, constituting a public health problem. Infested pigs must therefore be identified before they enter the food chain. To this end, a dot blot assay was developed for the immunodiagnosis of porcine cysticercosis. A study was made of 44 pigs from different areas of Colombia that had all tested positive to cysticercosis, both by necropsy and the Western blot technique. Another group was formed comprising 44 pigs that had all tested negative to Western blot and necropsy. After analysing these 88 samples to validate the diagnostic assay, the result was a sensitivity of 86.4% and a specificity of 93.2%. The dot blot assay proved useful in diagnosing porcine cysticercosis. As the assay is easy to use in laboratories in endemic areas, as well as under field conditions, it is also appropriate for epidemiological studies.
Agudelo-Flórez
P
P
Grupo de Investigación Medicina Tropical, Instituto Colombiano de Medicina Tropical, Universidad CES, Sabaneta, Colombia. pagudelo@ces.edu.co
Palacio
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LG
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D003551:Cysticercosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D003552:Cysticercus / Q000276:immunology*; D015151:Immunoblotting / Q000662:veterinary*; D015995:Prevalence; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D000909:Antibodies, Helminth