Constriction of the vasa recta, the vessels supplying the area at risk for acute kidney injury, by four different iodinated contrast media, evaluating ionic, nonionic, monomeric and dimeric agents.
OBJECTIVE
Iodinated contrast media (CM) can potentially cause contrast-induced nephropathy (CIN). It is not clear, however, whether particular types of CM are more prone to cause CIN than others. In this study we compare 4 types of CM (ionic vs. nonionic; monomer vs. dimer) on their effects on the microvessels that supply the area at risk for renal damage in CIN (outer medullary descending vasa recta-DVR).


METHODS
Using microdissection techniques, single DVR were isolated from rats and perfused using a set of concentric pipettes. After stabilization, perfusate was exchanged for a buffered solution containing either vehicle, or amidotrizoate (an ionic/monomeric CM), ioxaglate (an ionic/dimeric CM), iopromide (a nonionic/monomeric CM), and iodixanol (a nonionic/dimeric CM). The final iodine concentration was 23 mg iodine/mL, a concentration similar to that expected for coronary interventions. At this dilution, properties of CM solutions like viscosity and osmolarity are similar to the vehicle solution. To rule out further influence of CM-osmolarity and viscosity, the DVR bath solution was kept isoosmolar to the perfusate. Angiotensin II dose response curves were performed after the 20 minutes of perfusion. Digital videomicroscopy was used for measurements of luminal diameter.


RESULTS
All types of CM reduced luminal diameter of perfused DVR in a similar manner. After 20 minutes of perfusion, size of DVR were: 45% +/- 7% of initial diameter for the amidotrizoate-group; 53% +/- 6% for the ioxaglate-group; 63% +/- 11% for the iopromide-group; and 49% +/- 8% for the iodixanol-group. Control group remained at 96% +/- 4% of initial diameter. The angiotensin II dose response curves showed greater reactivity for amidotrizoate, iopromide and iodixanol, when compared with controls.


CONCLUSIONS
Under conditions where effects of osmolarity and viscosity are kept insignificant, perfusion of DVR using different types of iodinated CM leads to similar constriction of DVR. The response to angiotensin II was enhanced in 3 of the tested CM. This may be an important mechanism in the pathophysiology of CIN.
Sendeski
Mauricio
M
Department of Vessel Physiology Group, Institute of Vegetative Physiology, Charité-Universitätsmedizin Berlin, Berlin, Germany. mauricio.sendeski@charite.de
Patzak
Andreas
A
Persson
Pontus B
PB
D000704:Analysis of Variance; D000804:Angiotensin II / Q000633:toxicity; D000818:Animals; D003287:Contrast Media / Q000009:adverse effects* / Q000145:classification; D003973:Diatrizoate / Q000009:adverse effects / Q000145:classification; D006801:Humans; D007472:Iohexol / Q000009:adverse effects / Q000031:analogs & derivatives / Q000145:classification; D007485:Ioxaglic Acid / Q000009:adverse effects / Q000145:classification; D007668:Kidney / Q000098:blood supply / Q000187:drug effects*; D007674:Kidney Diseases / Q000139:chemically induced*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014283:Triiodobenzoic Acids / Q000009:adverse effects / Q000145:classification; D014661:Vasoconstriction / Q000187:drug effects*
D003287:Contrast Media; D014283:Triiodobenzoic Acids; D000804:Angiotensin II; D003973:Diatrizoate; D007472:Iohexol; C038192:iopromide; C044834:iodixanol; D007485:Ioxaglic Acid