title: FTIR study on the light sensitivity of the [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F: Ni-C to Ni-L photoconversion, kinetics of proton rebinding and H/D isotope effect.
abstract: The light-induced Ni-C to Ni-L transition results in the dissociation of a hydrogenic species, originating from the dihydrogen splitting at the active site. Back conversion in the dark to form Ni-C was investigated by studying the rebinding kinetics of this ligand in protonated (H(2)/H(2)O) and deuterated (D(2)/D(2)O) samples using time resolved FTIR spectroscopy.
authors.affiliation: Max-Planck-Institut für Bioanorganische Chemie, Stiftstrasse 34-36, D-45470 Mülheim an der Ruhr, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Kellers
authors.forename: Maria-Eirini
authors.initials: ME
authors.lastname: Pandelia
authors.forename: Leslie J
authors.initials: LJ
authors.lastname: Currell
authors.forename: Helmut
authors.initials: H
authors.lastname: Görner
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Lubitz
mesh_terms: D020134:Catalytic Domain; D016969:Desulfovibrio vulgaris / Q000201:enzymology*; D003903:Deuterium / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D006864:Hydrogenase / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008027:Light; D008958:Models, Molecular; D010777:Photochemistry; D011522:Protons; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D011522:Protons; D014867:Water; D006859:Hydrogen; D003903:Deuterium; C043296:nickel-iron hydrogenase; D006864:Hydrogenase