[Remyelination properties of human embryonic nerve cells in conditions of long-term culture].
We have examined the mitogenic and differentiation potential and remyelination properties of human embryonic nerve cells in culture. After 1 month of cultivation without differentiation agents CNP-positive cells (the mitotically-active precursors of oligodendrocytes) were expanded at 3,6 times. At the same time the amount of GalC-positive cells (mature oligodendrocytes) remained low. So, the remyelination properties of embryonic nerve cells can be explained by high concentration of oligodendrocytes precursors. Cell population after cultivation maintained the increased remyelination potential by increasing the number of CNP-positive cells that was confirmed by using experimental demyelination.
Tsymbaliuk
V I
VI
Vasyl'ieva
I H
IH
Oleksenko
N P
NP
Chopyk
N H
NH
Tsiubko
O I
OI
Halanta
O S
OS
D001921:Brain / Q000166:cytology* / Q000196:embryology; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D003470:Culture Media; D003711:Demyelinating Diseases / Q000628:therapy; D005699:Galactosylceramides / Q000378:metabolism; D006801:Humans; D008938:Mitosis / Q000502:physiology; D009186:Myelin Sheath / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D033581:Stem Cell Transplantation; D013997:Time Factors
D003470:Culture Media; D005699:Galactosylceramides; C002454:galactocerebroside