title: Purification of FANCD2 sub-complexes.
abstract: Fanconi anaemia (FA) is a recessive genetic disorder characterized by bone marrow failure, birth defects and cancer. Cells from FA patients are particularly defective in removing DNA interstrand crosslinks. We have developed a working chromatography purification scheme for FANCD2, a pivotal player in the FA DNA repair pathway, to facilitate identification of FANCD2 interacting partners. In doing so, at least three distinct FANCD2 subcomplexes were found to be present, designated as large, middle, and small complexes. The small complex is composed of tetramer of FANCD2 polypeptides, which may be the building block for other FANCD2 subcomplexes.
authors.affiliation: Department of Microbiology, University of Virginia Health System, Virginia, Charlottesville, VA, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhi
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Chen
authors.forename: William
authors.initials: W
authors.lastname: Newcomb
authors.forename: Jay
authors.initials: J
authors.lastname: Brown
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: Semmes
authors.forename: Gary M
authors.initials: GM
authors.lastname: Kupfer
mesh_terms: D002850:Chromatography, Gel / Q000379:methods; D005199:Fanconi Anemia / Q000378:metabolism*; D052236:Fanconi Anemia Complementation Group D2 Protein / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D008854:Microscopy, Electron
chemical_list: C499905:FANCD2 protein, human; D052236:Fanconi Anemia Complementation Group D2 Protein
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