[Expression of BDNF mRNA in nasal mucosa with allergic rhinitis rat model].
OBJECTIVE
To evaluate the role of brain-derived neurotrophic factor (BDNF) in allergic rhinitis of rat.


METHODS
Thirty SD rats free of disease were randomly divided into two groups. A model of allergic rhinitis rat was established by using ovalbumin intraperitoneal immunization and intranasal antigen challenge. The nasal mucosa from 18 out of 20 AR models as well as 10 normal controls were studied for expression of BDNF mRNA by reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
BDNF/beta-actin ratio in AR models was significantly higher than control (0.49+/-0.07 vs 0.28+/-0.01, P<0.05).


CONCLUSIONS
BDNF played an important role in the development of allergic rhinitis of rat.
Tao
Feng
F
Department of Otorhinolaryngology, Zhongda Hospital, Southeast University, Nanjing, 210009, China.
Zhang
Fengbing
F
Huang
Zhichun
Z
Sun
Baobin
B
Feng
Xu
X
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D009297:Nasal Mucosa / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012221:Rhinitis, Allergic, Perennial / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger