[Protein micro-crystallography with a new micro-beam beamline].
Recently, researchers' demands for high-quality data collection from protein micro-crystals, which would usually result from crystallization difficulty, have been remarkably growing. In order to collect efficient diffraction data for structure determination from micro-crystals, an expert data collection system providing high signal-to-noise ratio should be essential. A small sized and highly brilliant X-ray beam with size of a few micrometers has been proved to achieve protein micro-crystallography by both increasing reflection intensities and reducing background scattering from sample environments. Two micro-beam beamlines at SPring-8 and KEK-PF are currently under construction for Targeted Proteins Research Program by MEXT of Japan. At SPring-8, a new undulator beamline for protein micro-crystallography, named RIKEN Targeted Proteins Beamline (BL32XU), will start operation in May, 2010. The beam size at sample position of BL32XU will correspond to 1 x 1 microm(2) with the photon flux density over 6 x 10(10) photons/s/microm(2). Furthermore, research and developments of indispensable components to achieve protein micro-crystallography with micro-beam, such as high-precision diffractometer, automated micro-crystal handling, advanced data collection system to suppress radiation damage and so on, are progressing. The present status of the research and development for protein micro-crystallography will be presented.
Yamamoto
Masaki
M
RIKEN SPring-8 Center, Hyogo, Japan. yamamoto@riken.jp
Hirata
Kunio
K
Hikima
Takaaki
T
Kawano
Yoshiaki
Y
Ueno
Go
G
D003460:Crystallization; D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods*; D010316:Particle Size; D011506:Proteins / Q000737:chemistry*; D017356:Synchrotrons / Q000295:instrumentation*
D011506:Proteins