title: Epigenetic regulation of miR-184 by MBD1 governs neural stem cell proliferation and differentiation.
abstract: Methyl-CpG binding protein 1 (MBD1) regulates gene expression via a DNA methylation-mediated epigenetic mechanism. We have previously demonstrated that MBD1 deficiency impairs adult neural stem/progenitor cell (aNSC) differentiation and neurogenesis, but the underlying mechanism was unclear. Here, we show that MBD1 regulates the expression of several microRNAs in aNSCs and, specifically, that miR-184 is directly repressed by MBD1. High levels of miR-184 promoted proliferation but inhibited differentiation of aNSCs, whereas inhibition of miR-184 rescued the phenotypes associated with MBD1 deficiency. We further found that miR-184 regulates the expression of Numblike (Numbl), a known regulator of brain development, by binding to the 3'-UTR of Numbl mRNA and affecting its translation. Expression of exogenous Numbl could rescue the aNSC defects that result from either miR-184 overexpression or MBD1 deficiency. Therefore, MBD1, miR-184, and Numbl form a regulatory network that helps control the balance between proliferation and differentiation of aNSCs.
authors.affiliation: Department of Neurosciences, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
authors.forename: Changmei
authors.initials: C
authors.lastname: Liu
authors.forename: Zhao-Qian
authors.initials: ZQ
authors.lastname: Teng
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Santistevan
authors.forename: Keith E
authors.initials: KE
authors.lastname: Szulwach
authors.forename: Weixiang
authors.initials: W
authors.lastname: Guo
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhao
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000378:metabolism*; D044127:Epigenesis, Genetic; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C552233:MIRN184 microRNA, mouse; C489323:Mbd1 protein, mouse; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; C106292:Numbl protein, mouse
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