title: Decolorization and biodegradation of reactive blue 220 textile dye by Lentinus crinitus extracellular extract.
abstract: Studies were carried on the decolorization of the textile dye reactive blue 220 (RB220) by a novel isolate of Lentinus crinitus fungi. The optimal conditions for the production of destaining activity were obtained in media containing intermediate concentrations of ammonium oxalate and glucose (10 g L(-1)) as nitrogen and carbon sources, respectively, at 28 degrees C and pH 5.5. Maximum decolorization efficiency against RB220 achieved in this study was around 95%. Ultra-violet and visible (UV-vis) spectrophotometric analyses, before and after decolorization, suggest that decolorization was due to biodegradation. This effect was associated with a putative low molecular weight laccase (41 kDa) displaying good tolerance to a wide range of pH values, salt concentrations and temperatures, suggesting a potential role for this organism in the remediation of real dye containing effluents.
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Paraná, Setor Ciências Biológicas, Centro Politécnico, Caixa Postal, CEP 81531990, Curitiba, Paraná, Brazil.
authors.forename: Carolina Heyse
authors.initials: CH
authors.lastname: Niebisch
authors.forename: Alexandre Knoll
authors.initials: AK
authors.lastname: Malinowski
authors.forename: Ruth
authors.initials: R
authors.lastname: Schadeck
authors.forename: David A
authors.initials: DA
authors.lastname: Mitchell
authors.forename: Vanessa
authors.initials: V
authors.lastname: Kava-Cordeiro
authors.forename: Jaime
authors.initials: J
authors.lastname: Paba
mesh_terms: D001673:Biodegradation, Environmental; D002244:Carbon; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D003300:Copper / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D020074:Lentinula / Q000378:metabolism*; D009584:Nitrogen; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013784:Textiles
chemical_list: D004396:Coloring Agents; D002244:Carbon; D003300:Copper; D009584:Nitrogen