Yin Yang 1 phosphorylation contributes to the differential effects of mu-opioid receptor agonists on microRNA-190 expression.
Mu-opioid receptor regulates microRNA-190 (miR-190) in an agonist-dependent manner; fentanyl, but not morphine, decreases the miR-190 level in rat primary hippocampal neuron cultures and in mouse hippocampi. In this study, the correlation between the cellular content of miR-190 and the mRNA level of its host gene, talin2, suggested that fentanyl decreases the miR-190 level by inhibiting the transcription of talin2. Fentanyl-induced beta-arrestin2-mediated ERK phosphorylation led to the phosphorylation of Yin Yang 1 (YY1). In addition, YY1 phosphorylation impaired the association of YY1 with the -208 to -200 region on the Talin2 promoter, and this association was essential for YY1 to stimulate the transcription of talin2. Thus, fentanyl decreased the transcription of talin2 and subsequently the cellular level of miR-190 by inducing YY1 phosphorylation. In contrast, because morphine induces ERK phosphorylation via the protein kinase C pathway, morphine did not induce YY1 phosphorylation and had no effect on the transcription of talin2 and the cellular content of miR-190. This study therefore delineates a signaling pathway that mediates the effects of fentanyl on miR-190 expression.
Zheng
Hui
H
Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455-0217, USA. zhen0091@umn.edu
Chu
Ji
J
Zeng
Yan
Y
Loh
Horace H
HH
Law
Ping-Yee
PY
D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005283:Fentanyl / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D009020:Morphine / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017450:Receptors, Opioid, mu / Q000819:agonists* / Q000378:metabolism; D016608:Talin / Q000235:genetics / Q000378:metabolism; D050986:YY1 Transcription Factor / Q000378:metabolism*
D051792:Basic Helix-Loop-Helix Proteins; D004247:DNA; D048049:Extracellular Signal-Regulated MAP Kinases; D005283:Fentanyl; D035683:MicroRNAs; D009020:Morphine; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D017450:Receptors, Opioid, mu; D016608:Talin; D050986:YY1 Transcription Factor; C551254:MIRN190 microRNA, rat; C093564:Neurogenic differentiation factor 1
J Neurosci. 1998 Dec 1;18(23):9835-44
9822742
Nat Cell Biol. 2003 Aug;5(8):694-7
12894175
Neuron. 1998 Sep;21(3):593-606
9768845
J Cell Sci. 2007 Jan 15;120(Pt 2):213-8
17215450
Genes Dev. 2006 Mar 1;20(5):515-24
16510870
IUBMB Life. 2010 Feb;62(2):112-9
20058265
Gene. 1999 Aug 20;236(2):197-208
10452940
EMBO J. 2007 Dec 12;26(24):5093-108
18007592
Mol Cell Biol. 1999 Oct;19(10):7237-44
10490658
Oncogene. 2006 Feb 23;25(8):1125-42
16314846
Cell Motil Cytoskeleton. 2007 Mar;64(3):157-73
17183545
Biochemistry. 2004 Dec 14;43(49):15418-28
15581353
J Neurochem. 2006 Mar;96(6):1696-707
16539685
Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6418-23
17404223
J Neurosci. 1996 Jul 1;16(13):4102-12
8753872
Neuron. 2003 May 8;38(3):447-60
12741991
Mol Pharmacol. 2010 Jan;77(1):102-9
19854889
Cell Mol Life Sci. 2008 Oct;65(20):3265-89
18695942
Gene. 1994 Dec 15;150(2):259-66
7821790
J Neurochem. 2008 Aug;106(4):1493-502
18485096
Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7579-84
10840056
Neural Dev. 2008 Nov 05;3:29
18986510
Trends Pharmacol Sci. 2007 Aug;28(8):416-22
17644195
Cell. 2009 Oct 30;139(3):535-46
19879840
Nucleic Acids Res. 2008 Jan;36(Database issue):D154-8
17991681
Proc Natl Acad Sci U S A. 2008 Jul 8;105(27):9421-6
18599439
Genome Res. 2004 Oct;14(10A):1902-10
15364901
Oncogene. 2006 Oct 9;25(46):6156-62
17028594
Nat Rev Neurosci. 2006 Dec;7(12):911-20
17115073
Nature. 2006 Jan 19;439(7074):283-9
16421561
Biochem Biophys Res Commun. 2001 Sep 7;286(5):880-5
11527381
Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5078-83
16549775
J Pharmacol Sci. 2006 Aug;101(4):267-70
16921236
Biochim Biophys Acta. 1997 Apr 18;1332(2):F49-66
9141463
Neuron. 2004 Jan 22;41(2):229-41
14741104
Mol Pharmacol. 2008 Jan;73(1):178-90
17947509
Curr Opin Neurobiol. 2007 Feb;17(1):15-21
17254765
Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1725-30
15659552
Mol Cell. 2004 Dec 22;16(6):861-5
15610730
Hippocampus. 2006;16(3):199-207
16411231
Cell. 2004 Jan 23;116(2):281-97
14744438
J Comp Neurol. 2001 Jun 25;435(2):156-70
11391638