title: Dual reporter system for in situ detection of plasmid transfer under aerobic and anaerobic conditions.
abstract: We designed a new genetic tool to detect plasmid transfer under anaerobic and aerobic conditions. The system is based on the T7 RNA polymerase gene and a T7 promoter-driven oxygen-independent green fluorescent protein, evoglow, alone or in combination with red fluorescent protein DsRed. Constructs are available as plasmids and mini-mariner transposons.
authors.affiliation: Department of Biological Sciences, Initiative for Bioinformatics and Evolutionary Studies, University of Idaho, P.O. Box 443051, Moscow, ID 83844-3051, USA.
authors.forename: Jaroslaw E
authors.initials: JE
authors.lastname: Król
authors.forename: Linda M
authors.initials: LM
authors.lastname: Rogers
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Krone
authors.forename: Eva M
authors.initials: EM
authors.lastname: Top
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D017123:Bacteriophage T7; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D012321:DNA-Directed RNA Polymerases; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development* / Q000821:virology; D005821:Genetic Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014764:Viral Proteins; D000097573:Red Fluorescent Protein
chemical_list: D004251:DNA Transposable Elements; D012321:DNA-Directed RNA Polymerases; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D014764:Viral Proteins; D000097573:Red Fluorescent Protein; C062210:bacteriophage T7 RNA polymerase
references.citation: J Bacteriol. 1986 Jun;166(3):959-65
references.pmid: 3519587
references.citation: Nat Med. 2004 Dec;10(12 Suppl):S122-9
references.pmid: 15577930
references.citation: FEMS Microbiol Ecol. 2002 Nov 1;42(2):187-97
references.pmid: 19709278
references.citation: J Bacteriol. 1972 Jul;111(1):294-5
references.pmid: 4591482
references.citation: Curr Opin Biotechnol. 2003 Jun;14(3):262-9
references.pmid: 12849778
references.citation: Nat Rev Microbiol. 2005 Sep;3(9):700-10
references.pmid: 16138098
references.citation: J Bacteriol. 1969 Nov;100(2):1091-104
references.pmid: 4902383
references.citation: J Gen Microbiol. 1984 Apr;130(4):951-7
references.pmid: 6145751
references.citation: Biotechniques. 1994 May;16(5):800-2
references.pmid: 8068328
references.citation: Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11984-9
references.pmid: 11050229
references.citation: Biochemistry. 1997 Jun 3;36(22):6786-91
references.pmid: 9184161
references.citation: J Mol Biol. 1998 Mar 27;277(2):309-16
references.pmid: 9514749
references.citation: Protein Expr Purif. 2007 Oct;55(2):325-33
references.pmid: 17716915
references.citation: Genes Dev. 1996 Mar 15;10(6):755-61
references.pmid: 8598301
references.citation: Nat Biotechnol. 2007 Apr;25(4):443-5
references.pmid: 17351616