Morphological and genetic diversity of symbiotic cyanobacteria from cycads.
The morphological and genetic diversity of cyanobacteria associated with cycads was examined using PCR amplification techniques and 16S rRNA gene sequence analysis. Eighteen symbiotic cyanobacteria were isolated from different cycad species. One of the symbiotic isolates was a species of Calothrix, a genus not previously reported to form symbioses with Cycadaceae family, and the remainder were Nostoc spp. Axenic cyanobacterial strains were compared by DNA amplification using PCR with either short arbitrary primers or primers specific for the repetitive sequences. Based on fingerprint patterns and phenograms, it was revealed that cyanobacterial symbionts exhibit important genetic diversity among host plants, both within and between cycad populations. A phylogenetic analysis based on 16S rRNA gene sequence analysis revealed that most of the symbiotic cyanobacterial isolates fell into well-separated clades.
Thajuddin
Nooruddin
N
Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli Tamilnadu, India.
Muralitharan
Gangatharan
G
Sundaramoorthy
Mariappan
M
Ramamoorthy
Rengasamy
R
Ramachandran
Srinivasan
S
Akbarsha
Mohamed Abdulkadar
MA
Gunasekaran
Muthukumaran
M
D016000:Cluster Analysis; D000458:Cyanobacteria / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D032462:Cycadopsida / Q000382:microbiology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S