Multiplex Amplicon Quantification (MAQ), a fast and efficient method for the simultaneous detection of copy number alterations in neuroblastoma.
BACKGROUND
Cancer genomes display characteristic patterns of chromosomal imbalances, often with diagnostic and prognostic relevance. Therefore assays for genome-wide copy number screening and simultaneous detection of copy number alterations in specific chromosomal regions are of increasing importance in the diagnostic work-up of tumors.


RESULTS
We tested the performance of Multiplex Amplicon Quantification, a newly developed low-cost, closed-tube and high-throughput PCR-based technique for detection of copy number alterations in regions with prognostic relevance for neuroblastoma. Comparison with array CGH and the established Multiplex Ligation-dependent Probe Amplification method on 52 neuroblastoma tumors showed that Multiplex Amplicon Quantification can reliably detect the important genomic aberrations.


CONCLUSIONS
Multiplex Amplicon Quantification is a low-cost and high-throughput PCR-based technique that can reliably detect copy number alterations in regions with prognostic relevance for neuroblastoma.
Kumps
Candy
C
Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium.
Van Roy
Nadine
N
Heyrman
Lien
L
Goossens
Dirk
D
Del-Favero
Jurgen
J
Noguera
Rosa
R
Vandesompele
Jo
J
Speleman
Frank
F
De Preter
Katleen
K
D056915:DNA Copy Number Variations; D006801:Humans; D009447:Neuroblastoma / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods*; D016133:Polymerase Chain Reaction
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