title: Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis.
abstract: Shoot branching and growth are controlled by phytohormones such as auxin and other components in Arabidopsis. We identified a mutant (igi1) showing decreased height and bunchy branching patterns. The phenotypes reverted to the wild type in response to RNA interference with the IGI1 gene. Histochemical analysis by GUS assay revealed tissue-specific gene expression in the anther and showed that the expression levels of the IGI1 gene in apical parts, including flowers, were higher than in other parts of the plants. The auxin biosynthesis component gene, CYP79B2, was up-regulated in igi1 mutants and the IGI1 gene was down-regulated by IAA treatment. These results indicated that there is an interplay regulation between IGI1 and phytohormone auxin. Moreover, the expression of the auxin-related shoot branching regulation genes, MAX3 and MAX4, was down-regulated in igi1 mutants. Taken together, these results indicate that the overexpression of the IGI1 influenced MAX pathway in the shoot branching regulation.
authors.affiliation: Department of Biotechnology and BK21 Research Team for Protein Activity Control, Chosun University, Gwangju 501-759, Korea.
authors.forename: Indeok
authors.initials: I
authors.lastname: Hwang
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Cheol Soo
authors.initials: CS
authors.lastname: Kim
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Park
authors.forename: Giri Raj
authors.initials: GR
authors.lastname: Tripathi
authors.forename: Seong-Ki
authors.initials: SK
authors.lastname: Kim
authors.forename: Hyeonsook
authors.initials: H
authors.lastname: Cheong
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D049308:Dioxygenases; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007211:Indoles / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D010105:Oxygenases / Q000235:genetics*; D010641:Phenotype; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D007211:Indoles; D010937:Plant Growth Regulators; C001446:indoleacrylic acid; C475746:MAX4 protein, Arabidopsis; D010105:Oxygenases; C494441:CCD7 protein, Arabidopsis; D049308:Dioxygenases
references.citation: Plant J. 2001 Sep;27(6):581-90
references.pmid: 11576441
references.citation: Nature. 2008 Sep 11;455(7210):195-200
references.pmid: 18690207
references.citation: Genes Dev. 2003 Jun 15;17(12):1469-74
references.pmid: 12815068
references.citation: Plant Cell. 2007 Feb;19(2):458-72
references.pmid: 17307924
references.citation: Chembiochem. 2009 Jan 26;10(2):221-3
references.pmid: 19072818
references.citation: Plant Physiol. 2009 May;150(1):482-93
references.pmid: 19321710
references.citation: Plant Physiol. 1992 Nov;100(3):1494-502
references.pmid: 16653149
references.citation: Plant Physiol. 2009 Apr;149(4):1929-44
references.pmid: 19218361
references.citation: Plant Cell. 1990 Nov;2(11):1071-80
references.pmid: 1983791
references.citation: Genes Dev. 2001 Jun 15;15(12):1577-88
references.pmid: 11410537
references.citation: Plant Physiol. 2000 Apr;122(4):1003-13
references.pmid: 10759496
references.citation: Nature. 2008 Sep 11;455(7210):189-94
references.pmid: 18690209
references.citation: Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8821-6
references.pmid: 15166290
references.citation: Planta. 1977 Jan;136(1):91-6
references.pmid: 24420232
references.citation: Development. 2005 Oct;132(20):4521-31
references.pmid: 16192309
references.citation: Plant Cell Physiol. 2004 Dec;45(12):1875-81
references.pmid: 15653807
references.citation: Plant Cell. 2005 Feb;17(2):444-63
references.pmid: 15659631
references.citation: Curr Biol. 2006 Mar 21;16(6):553-63
references.pmid: 16546078
references.citation: Plant J. 2007 Sep;51(6):1019-29
references.pmid: 17655651
references.citation: Plant Physiol. 2005 Jul;138(3):1665-72
references.pmid: 15965021
references.citation: J Biol Chem. 2009 Feb 20;284(8):5257-64
references.pmid: 19098002
references.citation: Am J Bot. 1997 Aug;84(8):1064
references.pmid: 21708661
references.citation: Plant Physiol. 1999 Nov;121(3):839-47
references.pmid: 10557232
references.citation: Plant Mol Biol. 2010 May;73(1-2):27-36
references.pmid: 20112050
references.citation: Curr Biol. 2004 Jul 27;14(14):1232-8
references.pmid: 15268852
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Genes Dev. 2002 Dec 1;16(23):3100-12
references.pmid: 12464638
references.citation: Curr Opin Plant Biol. 2004 Feb;7(1):73-8
references.pmid: 14732444
references.citation: Plant Physiol. 2006 Nov;142(3):812-9
references.pmid: 17093134
references.citation: Plant Cell. 2005 Mar;17(3):746-59
references.pmid: 15705953
references.citation: Plant Cell. 1989 Apr;1(4):403-413
references.pmid: 12359893
references.citation: Proc Natl Acad Sci U S A. 1933 Jul;19(7):714-6
references.pmid: 16577553
references.citation: Plant J. 1995 Sep;8(3):457-63
references.pmid: 7550382
references.citation: Science. 1998 Dec 18;282(5397):2226-30
references.pmid: 9856939
references.citation: Plant J. 2005 Nov;44(4):569-80
references.pmid: 16262707
references.citation: Dev Cell. 2005 Mar;8(3):443-9
references.pmid: 15737939
references.citation: Plant Physiol. 2009 Sep;151(1):400-12
references.pmid: 19641034
references.citation: Science. 2006 May 12;312(5775):914-8
references.pmid: 16601150
references.citation: Development. 2002 Mar;129(5):1131-41
references.pmid: 11874909
references.citation: Plant Physiol. 2001 Dec;127(4):1405-13
references.pmid: 11743082