Neutralizing antibodies to Shiga toxin type 2 (Stx2) reduce colonization of mice by Stx2-expressing Escherichia coli O157:H7.
Previously, we showed that the Shiga toxin type 2 (Stx2)-expressing Escherichia coli O157:H7 strain 86-24 colonized mice better than did its isogenic stx(2) negative mutant. Here, we confirmed that finding by demonstrating that Stx2 given orally to mice increased the levels of the 86-24 stx(2) mutant shed in feces. Then we assessed the impact of Stx2-neutralizing antibodies, administered passively or generated by immunization with an Stx2 toxoid, on E. coli O157:H7 colonization of mice. We found that such antibodies reduced the E. coli O157:H7 burden in infected mice and, as anticipated, also protected them from weight loss and death.
Mohawk
Krystle L
KL
Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
Melton-Celsa
Angela R
AR
Robinson
Cory M
CM
O'Brien
Alison D
AD
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control*; D019453:Escherichia coli O157 / Q000235:genetics* / Q000276:immunology*; D005243:Feces / Q000737:chemistry; D005260:Female; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011817:Rabbits; D022641:Shiga Toxin 2 / Q000235:genetics / Q000276:immunology*; D014121:Toxoids / Q000276:immunology / Q000627:therapeutic use; D014611:Vaccination; D015431:Weight Loss
D000907:Antibodies, Bacterial; D057134:Antibodies, Neutralizing; D022641:Shiga Toxin 2; D014121:Toxoids
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