title: Genetic interactions show the importance of rRNA modification machinery for the role of Rps15p during ribosome biogenesis in S. cerevisiae.
abstract: Rps15p, an essential ribosomal protein, was previously shown to be critical for nuclear export of small subunit pre-particles. We have designed a synthetic lethal screen in Saccharomyces cerevisiae to identify its genetic partners and further elucidate its role during ribosomal biogenesis. Our screen revealed interactions with mutants affected at various stages during ribosome biogenesis, from early nucleolar steps to nuclear export. Mutations were identified in genes encoding proteins involved in early ribosome biogenesis steps, like the small subunit processome component Utp15p, the 90S pre-ribosome factor Slx9p and the H/ACA snoRNP core protein Nhp2p. In addition, we found a synthetic lethality with BUD23, a gene encoding a methyltransferase involved both in rRNA modification and small subunit nuclear export. Interestingly, deletion of snR36 or snR85, two H/ACA snoRNAs that direct modifications close to Rps15p's binding site on the rRNA, produces mild and opposite effects on growth in an rps15 hypomorphic background. These data uncover an unreported link between a ribosomal protein and rRNA modification machinery.
authors.affiliation: Laboratoire de Biologie Moléculaire Eucaryote, Université de Toulouse, Toulouse, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Bellemer
authors.forename: Pauline
authors.initials: P
authors.lastname: Chabosseau
authors.forename: Franck
authors.initials: F
authors.lastname: Gallardo
authors.forename: Pierre-Emmanuel
authors.initials: PE
authors.lastname: Gleizes
authors.forename: Guillaume
authors.initials: G
authors.lastname: Stahl
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D005816:Genetic Complementation Test; D005820:Genetic Testing; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009690:Nucleic Acid Conformation; D010641:Phenotype; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020537:RNA, Small Nucleolar / Q000235:genetics; D012269:Ribosomal Proteins / Q000378:metabolism*; D054682:Ribosome Subunits, Small, Eukaryotic / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D012335:RNA, Ribosomal; D020537:RNA, Small Nucleolar; D012269:Ribosomal Proteins; C032643:ribosomal protein S21
references.citation: Mol Cell Biol. 2008 May;28(10):3151-61
references.pmid: 18332120
references.citation: J Cell Biol. 1990 Dec;111(6 Pt 1):2261-74
references.pmid: 2277060
references.citation: RNA. 2006 Nov;12(11):2005-13
references.pmid: 17018574
references.citation: Mol Cell. 2007 Dec 28;28(6):965-77
references.pmid: 18158895
references.citation: RNA. 2009 Sep;15(9):1716-28
references.pmid: 19628622
references.citation: Nature. 2002 Jun 27;417(6892):967-70
references.pmid: 12068309
references.citation: Mol Cell. 2004 May 7;14(3):331-42
references.pmid: 15125836
references.citation: EMBO J. 2001 Aug 1;20(15):4204-13
references.pmid: 11483523
references.citation: Genes Dev. 2004 Jan 15;18(2):196-209
references.pmid: 14729571
references.citation: Yeast. 1997 Jul;13(9):837-48
references.pmid: 9234672
references.citation: Genes Dev. 2009 Apr 15;23(8):963-74
references.pmid: 19390089
references.citation: EMBO J. 1998 Dec 1;17(23):7078-90
references.pmid: 9843512
references.citation: Genes Dev. 2004 Oct 15;18(20):2506-17
references.pmid: 15489292
references.citation: RNA. 2005 Jun;11(6):928-38
references.pmid: 15923376
references.citation: Mol Biol Cell. 2004 Nov;15(11):5038-46
references.pmid: 15356263
references.citation: J Mol Biol. 1988 Mar 20;200(2):309-19
references.pmid: 3373531
references.citation: Cell. 2005 Jun 3;121(5):713-24
references.pmid: 15935758
references.citation: Cell Mol Life Sci. 2008 Aug;65(15):2334-59
references.pmid: 18408888
references.citation: Mol Cell. 2005 Oct 28;20(2):263-75
references.pmid: 16246728
references.citation: Cell. 1997 May 30;89(5):799-809
references.pmid: 9182768
references.citation: EMBO J. 2005 Aug 17;24(16):2862-72
references.pmid: 16037817
references.citation: Int Rev Cytol. 2004;239:99-178
references.pmid: 15464853
references.citation: EMBO J. 1987 Dec 20;6(13):4169-75
references.pmid: 3327689
references.citation: EMBO J. 2004 Jun 16;23(12):2336-47
references.pmid: 15167894
references.citation: Genetics. 2006 Oct;174(2):679-91
references.pmid: 16888326
references.citation: J Cell Biol. 2001 Dec 10;155(6):923-36
references.pmid: 11739405
references.citation: Genetics. 1989 May;122(1):19-27
references.pmid: 2659436
references.citation: BMC Bioinformatics. 2002;3:2
references.pmid: 11869452
references.citation: J Mol Biol. 2002 Feb 22;316(3):725-68
references.pmid: 11866529