[Phenotypic and epigenetic adaptation of the moss clone to mercury].
On agar-Knop medium containing 0.5 microM HgCl2 about one third of microregenerants of the clone from the individual gametophyte cell of the moss Pottia intermedia survived and gave rise to protonemal mats. The high survival percentage testifies to epigenetic nature of adaptation. The latter proved to be correlated to the increase of leaf cell number and of peroxidase activity as well as to intensification of activity zone of peroxidase isoform with MM in limits of 66 kD and to appearance of two isoforms of the enzyme on electrophoregrams. The increase of peroxidase activity, though considerably weaker expressed, has been stated at 0.2 microM HgCl2 when practically all regenerants survived and on the mercury-free medium epigenetically adapted regenerants differed from physiologically adapted ones only in intensification of activity zone of peroxidase isoform with 66 kD. This gives reason to regard the adaptation of the regenerants to 0.5 microM HgCl2 as intensified epigenocopy of modification and indicates the generality of mechanisms of both types of adaptation.
Khorkavtsiv
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D000220:Adaptation, Biological / Q000187:drug effects / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004785:Environmental Pollutants / Q000633:toxicity*; D044127:Epigenesis, Genetic / Q000187:drug effects*; D055993:Germ Cells, Plant / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D007527:Isoenzymes; D008627:Mercuric Chloride / Q000633:toxicity*; D008970:Molecular Weight; D009195:Peroxidase / Q000378:metabolism; D010641:Phenotype; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D044003:Sphagnopsida / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development
D004785:Environmental Pollutants; D007527:Isoenzymes; D008627:Mercuric Chloride; D009195:Peroxidase