title: Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
abstract: Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via Agrobacterium-mediated transformation, and putative transgenic lines were developed. The presence of the transgene in these putative transgenic lines was determined using polymerase chain reaction (PCR), and transgene integration and transgene copy number were confirmed following Southern blot analysis. The presence of specific F1-V transcripts was confirmed by reverse-transcriptase (RT)-PCR. Using monoclonal antibodies, ELISA and western blot analysis revealed that the expected antigenic F1-V protein was successfully expressed in transgenic lines. Mice immunized subcutaneously with lettuce expressing the F1-V antigen developed systemic humoral responses as 'proof of concept' of using lettuce as a production platform for the F1-V immunogen that could be used as a candidate plant-based vaccine against plague.
authors.affiliation: Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosi, Mexico.
authors.forename: Sergio
authors.initials: S
authors.lastname: Rosales-Mendoza
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Soria-Guerra
authors.forename: Leticia
authors.initials: L
authors.lastname: Moreno-Fierros
authors.forename: Angel G
authors.initials: AG
authors.lastname: Alpuche-Solís
authors.forename: Luzmila
authors.initials: L
authors.lastname: Martínez-González
authors.forename: Schuyler S
authors.initials: SS
authors.lastname: Korban
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D018545:Lactuca / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010930:Plague / Q000276:immunology* / Q000382:microbiology; D010931:Plague Vaccine / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D052899:Pore Forming Cytotoxic Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics; D015010:Yersinia pestis / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D000942:Antigens, Bacterial; C043908:LcrV protein, Yersinia; D010931:Plague Vaccine; D052899:Pore Forming Cytotoxic Proteins; D011993:Recombinant Fusion Proteins
references.citation: Nat Med. 1998 May;4(5):607-9
references.pmid: 9585236
references.citation: Vaccine. 2000 Oct 15;19(4-5):566-71
references.pmid: 11027822
references.citation: Exp Biol Med (Maywood). 2006 Sep;231(8):1346-52
references.pmid: 16946403
references.citation: Curr Opin Biotechnol. 1998 Apr 1;9(2):227-32
references.pmid: 9664052
references.citation: Proc Natl Acad Sci U S A. 2006 Jan 24;103(4):861-6
references.pmid: 16410352
references.citation: Transgenic Res. 2006 Apr;15(2):205-17
references.pmid: 16604461
references.citation: Plant Cell Rep. 2004 May;22(10):711-20
references.pmid: 14997337
references.citation: Braz J Infect Dis. 2008 Dec;12(6):469-71
references.pmid: 19287831
references.citation: Adv Exp Med Biol. 2007;603:415-24
references.pmid: 17966437
references.citation: Vaccine. 2006 Mar 24;24(14):2477-90
references.pmid: 16442673
references.citation: Exp Cell Res. 1968 Apr;50(1):151-8
references.pmid: 5650857
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: J Infect Dis. 1974 May;129:Suppl:S13-8
references.pmid: 4596516
references.citation: Expert Rev Vaccines. 2008 Mar;7(2):209-21
references.pmid: 18324890
references.citation: Vaccine. 2005 Mar 7;23(15):1753-6
references.pmid: 15734036
references.citation: Plant Mol Biol. 2005 Aug;58(6):763-774
references.pmid: 16240172
references.citation: Plant Mol Biol. 2010 Jan;72(1-2):75-89
references.pmid: 19789982
references.citation: Methods Mol Biol. 2006;343:449-58
references.pmid: 16988367
references.citation: FASEB J. 1999 Oct;13(13):1796-9
references.pmid: 10506582
references.citation: Infect Immun. 2008 Aug;76(8):3640-50
references.pmid: 18505806
references.citation: Infect Immun. 2005 Jun;73(6):3598-608
references.pmid: 15908389
references.citation: Science. 1995 May 5;268(5211):714-6
references.pmid: 7732379