title: Novel carbohydrate-binding module identified in a ruminal metagenomic endoglucanase.
abstract: Endoglucanase C5614-1 comprises a catalytic module (CM) and an X module (XM). The XM showed no significant homology with known carbohydrate-binding modules (CBMs). Recombinant full-length endoglucanase could bind Avicel, whereas the CM could not. The XM could bind various polysaccharides. The results demonstrated that the XM was a new CBM.
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Cheng-Jie
authors.initials: CJ
authors.lastname: Duan
authors.forename: Jun-Liang
authors.initials: JL
authors.lastname: Liu
authors.forename: Xi
authors.initials: X
authors.lastname: Wu
authors.forename: Ji-Liang
authors.initials: JL
authors.lastname: Tang
authors.forename: Jia-Xun
authors.initials: JX
authors.lastname: Feng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002020:Buffaloes; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism*; D054892:Metagenome; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding; D012417:Rumen / Q000382:microbiology*; D016415:Sequence Alignment
chemical_list: D011134:Polysaccharides; D002480:Cellulase
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