[Molecular-genetic polymorphism of cellular lines of wheat resistant to cultural filtrate Gaeumannomyces graminis var. tritici and plant-regenerants from them].
DNA polymorphism of wheat cellular lines resistant to culture filtrate of G. graminis var. tritici and regenerated plants has been investigated using ISSR-analysis. Specific changes in DNA sequence were revealed in resistant calluses. It was established, that all resistant cellular lines differed by the presence of specific amplicons of 2347 bp (primer 5'-TCTCTCTCTCTCTCTCG-3') and 1745 bp (primer 5'-AGAGAGAGAGAGAGAGTC-3'), as well as the absence of the amplicon of 1108 bp (primer 5'-ACACACACACACACACC-3') from the initial callus and the callus not exposed to the action of selective factor. These changes have been also revealed in R0 and R1 plants.
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D001203:Ascomycota / Q000166:cytology / Q000254:growth & development*; D001483:Base Sequence; D046508:Culture Techniques; D018744:DNA, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology / Q000517:prevention & control*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012038:Regeneration; D014908:Triticum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D018744:DNA, Plant