title: High-oxygen modified atmosphere packaging system induces lipid and myoglobin oxidation and protein polymerization.
abstract: Beef steaks from longissimus lumborum, semimembranosus, and adductor muscles (n=10; respectively) were cut at 24h postmortem, randomly assigned to either high-oxygen modified atmosphere packaging (HiOx-MAP; 80% O(2), 20% CO(2)) or vacuum (VAC), and displayed for 9days at 1 degrees C. HiOx-MAP packaged beef steaks had a rapid increase in lipid oxidation and a decrease in color stability during display. The steaks in HiOx-MAP had significantly lower tenderness and juiciness scores, and higher off-flavor scores compared to steaks in VAC. HiOx-MAP condition did not affect the postmortem degradation of troponin-T or desmin. Furthermore, autolysis of micro-calpain was not influenced by packaging. SDS-PAGE, immunoblotting, and diagonal-PAGE revealed oxidative cross-linking of myosin heavy chain in meat packaged in HiOx-MAP. These results suggest that the HiOx-MAP system may negatively affect meat quality characteristics by inducing lipid and myoglobin oxidation and cross-linking/aggregation of myosin by protein oxidation.
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA 50011, United States.
authors.forename: Yuan H
authors.initials: YH
authors.lastname: Kim
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Huff-Lonergan
authors.forename: Joseph G
authors.initials: JG
authors.lastname: Sebranek
authors.forename: Steven M
authors.initials: SM
authors.lastname: Lonergan
mesh_terms: D000818:Animals; D001329:Autolysis; D002154:Calpain / Q000378:metabolism; D002417:Cattle; D003116:Color; D003893:Desmin / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005511:Food Handling / Q000379:methods*; D005519:Food Preservation / Q000379:methods*; D005524:Food Technology; D006801:Humans; D015151:Immunoblotting; D015227:Lipid Peroxidation; D008460:Meat / Q000592:standards*; D018482:Muscle, Skeletal / Q000378:metabolism; D009211:Myoglobin / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen; D011108:Polymers; D011180:Postmortem Changes; D050050:Protein Carbonylation; D011897:Random Allocation; D013649:Taste; D020107:Troponin T / Q000378:metabolism; D014618:Vacuum
chemical_list: D003893:Desmin; D009211:Myoglobin; D011108:Polymers; D020107:Troponin T; D002154:Calpain; D018995:Myosin Heavy Chains; D010100:Oxygen