Measurement of vulnerability to water stress-induced cavitation in grapevine: a comparison of four techniques applied to a long-vesseled species.
Among woody plants, grapevines are often described as highly vulnerable to water-stress induced cavitation with emboli forming at slight tensions. However, we found native embolism never exceeded 30% despite low xylem water potentials (Psi(x)) for stems of field grown vines. The discrepancy between native embolism measurements and those of previous reports led us to assess vulnerability curve generation using four separate methods and alterations (i.e. segment length and with/without flushing to remove embolism prior to measurement) of each. Centrifuge, dehydration and air-injection methods, which rely on measurement of percentage loss of hydraulic conductivity (PLC) in detached stems, were compared against non-invasive monitoring of xylem cavitation with nuclear magnetic resonance (NMR) imaging. Short segment air-injection and flushed centrifuge stems reached >90 PLC at Psi(x) of-0.5 and -1.5 MPa, respectively, whereas dehydration and long-segment air-injection measurements indicated no significant embolism at Psi(x) > -2.0 MPa. Observations from NMR agreed with the dehydration and long segment air-injection methods, showing the majority of vessels were still water-filled at Psi(x) > -1.5 MPa. Our findings show V. vinifera stems are far less vulnerable to water stress-induced cavitation than previously reported, and dehydration and long segment air-injection techniques are more appropriate for long-vesseled species and organs.
Choat
Brendan
B
Plant Science Division, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia.
Drayton
William M
WM
Brodersen
Craig
C
Matthews
Mark A
MA
Shackel
Ken A
KA
Wada
Hiroshi
H
McElrone
Andrew J
AJ
D002498:Centrifugation / Q000379:methods; D003681:Dehydration; D003890:Desiccation / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D018547:Plant Stems / Q000502:physiology; D027843:Vitis / Q000502:physiology*; D014867:Water / Q000502:physiology*; D052584:Xylem / Q000502:physiology*
D014867:Water