title: Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor.
abstract: We report the one-pot amplification and isolation of a nanomolar receptor in a multibuilding block aqueous dynamic combinatorial library using a polymer-bound template. By appropriate choice of a poly(N,N-dimethylacrylamide)-based support, nonselective ion-exchange type behaviour between the oppositely charged cationic guest and polyanionic hosts was overcome, such that the selective molecular recognition arising in aqueous solution reactions is manifest also in the analogous templated solid phase DCL syntheses. The ability of a polymer bound template to identify and isolate a synthetic receptor via dynamic combinatorial chemistry was not compromised by the large size of the library, consisting of well over 140 theoretical members, demonstrating the practical advantages of a polymer-supported DCL methodology.
authors.affiliation: WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UK G1 1XL.
authors.forename: Pol
authors.initials: P
authors.lastname: Besenius
authors.forename: Peter A G
authors.initials: PA
authors.lastname: Cormack
authors.forename: R Frederick
authors.initials: RF
authors.lastname: Ludlow
authors.forename: Sijbren
authors.initials: S
authors.lastname: Otto
authors.forename: David C
authors.initials: DC
authors.lastname: Sherrington
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000547:Amantadine / Q000737:chemistry; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004220:Disulfides / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000178:Acrylamides; D004220:Disulfides; C429790:poly(N,N-dimethylacrylamide); D014867:Water; D000547:Amantadine