Constructing an improved pyrroloquinoline quinone glucose dehydrogenase binding aptamer for enzyme labeling.
Motif analysis of PGa4 indicated that eight consecutive guanine residues were most important for pyrroloquinoline quinine glucose dehydrogenase (PQQGDH) recognition. Because the truncated mutants minimized unexpected interactions between PGa4 and the target-binding aptamer, they will now facilitate the construction of a highly sensitive detection system using a PQQGDH-labeled diagnostic marker-binding aptamer.
Abe
Koichi
K
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, Japan.
Sode
Koji
K
Ikebukuro
Kazunori
K
D052157:Aptamers, Nucleotide / Q000378:metabolism*; D005948:Glucose Dehydrogenases / Q000378:metabolism*; D011485:Protein Binding; D013194:Staining and Labeling / Q000379:methods*
D052157:Aptamers, Nucleotide; D005948:Glucose Dehydrogenases; C033955:glucose dehydrogenase (pyrroloquinoline-quinone)