Proteins required for lipopolysaccharide assembly in Escherichia coli form a transenvelope complex.
The viability of Gram-negative organisms is dependent on the proper placement of lipopolysaccharide (LPS) in the outer leaflet of its outer membrane. LPS is synthesized inside the cell and transported to the surface by seven essential lipopolysaccharide transport (Lpt) proteins. How these proteins cooperate to transport LPS is unknown. We show that these Lpt proteins can be found in a membrane fraction that contains inner and outer membranes and that they copurify. This constitutes the first evidence that the Lpt proteins form a transenvelope complex. We suggest that this protein bridge provides a route for LPS transport across the cell envelope.
Chng
Shu-Sin
SS
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Gronenberg
Luisa S
LS
Kahne
Daniel
D
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000502:physiology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000502:physiology; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000502:physiology; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*
D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D008070:Lipopolysaccharides; C532155:LptA protein, E coli; C543317:LptB protein, E coli; C543318:LptC protein, E coli; C529275:LptF protein, E coli; C529276:LptG protein, E coli; D008565:Membrane Proteins; D046912:Multiprotein Complexes; C043426:inner membrane protein, E coli
Proc Natl Acad Sci U S A. 2008 Apr 8;105(14):5537-42
18375759
Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):13823-8
18768814
J Bacteriol. 2007 Jan;189(1):244-53
17056748
J Biol Chem. 1986 Jan 5;261(1):428-43
3510202
J Bacteriol. 2008 Jul;190(13):4460-9
18424520
Biochemistry. 1976 Jun 15;15(12):2561-70
820368
Annu Rev Biochem. 2002;71:635-700
12045108
Eur J Biochem. 1975 Feb 21;51(2):343-52
807474
Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5363-8
20203010
Mol Microbiol. 2002 Sep;45(5):1289-302
12207697
Eur J Biochem. 1973 Jun 15;35(3):471-81
4581267
J Gen Microbiol. 1968 Oct;53(3):395-404
4181162
Science. 2010 Feb 19;327(5968):1010-3
20167788
Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11754-9
16861298
Nat Rev Microbiol. 2009 Sep;7(9):677-83
19633680
J Biol Chem. 2005 Feb 11;280(6):4504-9
15576375
J Am Chem Soc. 2010 Mar 3;132(8):2518-9
20136079
Biosci Biotechnol Biochem. 2009 Mar 23;73(3):465-73
19270402
J Mol Biol. 2008 Jul 11;380(3):476-88
18534617
Biochim Biophys Acta. 2009 Jul;1791(7):594-602
19416651
J Struct Biol. 1991 Dec;107(3):268-80
1807357
Cell. 2005 Apr 22;121(2):235-45
15851030
J Biol Chem. 2008 Jul 18;283(29):20342-9
18480051
Microbiol Mol Biol Rev. 2003 Dec;67(4):593-656
14665678
Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9417-22
15192148
Nucleic Acids Res. 2008 Jan;36(Database issue):D281-8
18039703
Annu Rev Microbiol. 2007;61:191-214
17506684
FEBS Lett. 2009 Jul 7;583(13):2160-4
19500581