The cyclic AMP (cAMP)-cAMP receptor protein signaling system mediates resistance of Vibrio cholerae O1 strains to multiple environmental bacteriophages.
Toxigenic Vibrio cholerae, the causative agent of the epidemic diarrheal disease cholera, interacts with diverse environmental bacteriophages. These interactions promote genetic diversity or cause selective enrichment of phage-resistant bacterial clones. To identify bacterial genes involved in mediating the phage-resistant phenotype, we screened a transposon insertion library of V. cholerae O1 El Tor biotype strain C6706 to identify mutants showing altered susceptibility to a panel of phages isolated from surface waters in Bangladesh. Mutants with insertion in cyaA or crp genes encoding adenylate cyclase or cyclic AMP (cAMP) receptor protein (CRP), respectively, were susceptible to a phage designated JSF9 to which the parent strain was completely resistant. Application of the cyaA mutant as an indicator strain in environmental phage monitoring enhanced phage detection, and we identified 3 additional phages to which the parent strain was resistant. Incorporation of the cyaA or crp mutations into other V. cholerae O1 strains caused similar alterations in their phage susceptibility patterns, and the susceptibility correlated with the ability of the bacteria to adsorb these phages. Our results suggest that cAMP-CRP-mediated downregulation of phage adsorption may contribute to a mechanism for the V. cholerae O1 strains to survive predation by multiple environmental phages. Furthermore, the cyaA or crp mutant strains may be used as suitable indicators in monitoring cholera phages in the water.
Zahid
M Shamim Hasan
MS
Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh.
Waise
T M Zaved
TM
Kamruzzaman
M
M
Ghosh
Amar N
AN
Nair
G Balakrish
GB
Mekalanos
John J
JJ
Faruque
Shah M
SM
D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism; D001435:Bacteriophages / Q000302:isolation & purification / Q000502:physiology*; D001459:Bangladesh; D002373:Cyclic AMP Receptor Protein / Q000235:genetics* / Q000378:metabolism*; D015536:Down-Regulation; D009154:Mutation; D045483:Rivers / Q000821:virology*; D015398:Signal Transduction; D041241:Vibrio cholerae O1 / Q000502:physiology / Q000821:virology*
D002373:Cyclic AMP Receptor Protein; D000262:Adenylyl Cyclases
Nat Rev Microbiol. 2009 Oct;7(10):693-702
19756008
Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):265-70
8990197
Arch Biochem Biophys. 1976 Jul;175(1):295-302
182080
Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11951-6
18689675
Microbiol Mol Biol Rev. 1998 Dec;62(4):1301-14
9841673
Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4652-7
16537404
Microbiol Mol Biol Rev. 2004 Sep;68(3):560-602, table of contents
15353570
Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1702-7
15653771
Clin Microbiol Rev. 1995 Jan;8(1):48-86
7704895
DNA Cell Biol. 2008 Jul;27(7):347-55
18462070
Microbiol Sci. 1987 Jul;4(7):214-8
3153614
J Mol Biol. 2008 Oct 31;383(1):1-9
18619465
J Clin Microbiol. 1996 Jul;34(7):1843-5
8784608
J Bacteriol. 1978 Jan;133(1):149-57
201606
Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):6119-24
15829587
Mol Microbiol. 2002 Mar;43(6):1577-89
11952906
Infect Immun. 2008 Nov;76(11):5266-73
18794293
J Bacteriol. 2008 Oct;190(20):6646-59
18708497
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2258-62
4332251
Infect Immun. 2006 Jun;74(6):3633-42
16714595
Science. 1996 Jun 28;272(5270):1910-4
8658163
J Bacteriol. 1978 Apr;134(1):356-8
206537
Science. 2005 Dec 16;310(5755):1824-7
16357262
FEMS Microbiol Lett. 2004 Mar 12;232(1):1-6
15061140
Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8736-41
18574146