Biodegradation of bisphenol A and its halogenated analogues by Cunninghamella elegans ATCC36112.
Bisphenol A and its halogenated analogues are commonly used industrial chemicals with strong toxicological effects over many organisms. In this study, metabolic fate of bisphenol A and its halogenated analogues were evaluated with Cunninghamella elegans ATCC36112. Bisphenol A and related analogues were rapidly transformed into several metabolites by C. elegans within 2-4 days. Detailed analysis of metabolites reveals that both phase I and II metabolism occurred in C. elegans. Cytochrome P450-dependent hydroxylation was observed in BPA. However, major reaction with bisphenol A and analogues with 1-2 halogen atoms were the formation of glucose-conjugate, not being inhibited by cytochrome P450 inhibitor. Overall metabolic rates decreased with increasing number of substitution at 2- and 6-position of BPA structures, which may be consequences of limited bioavailability or steric hindrance to conjugate-forming reaction. Information from the current study will provide detailed insights over the fungal metabolism of BPA and analogues.
Keum
Young Soo
YS
Department of Agricultural Biotechnology, Seoul National University, Gwanak-Gu, Seoul, Republic of Korea.
Lee
Hye Ri
HR
Park
Hee Won
HW
Kim
Jeong-Han
JH
D001559:Benzhydryl Compounds; D001673:Biodegradation, Environmental; D020093:Cunninghamella / Q000201:enzymology / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D007700:Kinetics; D010636:Phenols / Q000737:chemistry* / Q000378:metabolism*; D010882:Piperonyl Butoxide / Q000378:metabolism; D013997:Time Factors; D001617:beta-Glucosidase / Q000378:metabolism; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D010636:Phenols; D010882:Piperonyl Butoxide; D001617:beta-Glucosidase; C006780:bisphenol A; D000098826:Bisphenol A Compounds