Molecular identification of the vaccine strain from the inactivated oil emulsion H9N2 low pathogenic avian influenza vaccine.
In order to control the H9N2 subtype low pathogenic avian influenza (LPAI), an inactivated vaccine has been used in Korea since 2007. The Korean veterinary authority permitted the use of a single H9N2 LPAI vaccine strain to simplify the evolution of the circulating virus due to the immune pressure caused by the vaccine use. It is therefore important to determine the suitability of the vaccine strain in the final inactivated oil emulsion LPAI vaccine. In this study, we applied molecular rather than biological methods to verify the suitability of the vaccine strain used in commercial vaccines and successfully identified the strain by comparing the nucleotide sequences of the hemagglutinin and neuraminidase genes with that of the permitted Korean LPAI vaccine strain. It is thought that the method used in this study might be successfully applied to other viral genes of the LPAI vaccine strain and perhaps to other veterinary oil emulsion vaccines.
Choi
Jun Gu
JG
National Veterinary Research and Quarantine Service, Anyang 430-757, Korea.
Lee
Youn Jeong
YJ
Kim
Ji Yeon
JY
Kim
Yeon Hee
YH
Paek
Mi Ra
MR
Yang
Dong Kun
DK
Son
Seong Wan
SW
Kim
Jae Hong
JH
D000818:Animals; D001483:Base Sequence; D001717:Birds; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D056910:Republic of Korea; D016415:Sequence Alignment; D015164:Vaccines, Inactivated / Q000235:genetics / Q000276:immunology
D004279:DNA, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated; D009439:Neuraminidase
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