title: poly is required for nurse-cell chromosome dispersal and oocyte polarity in Drosophila.
abstract: During Drosophila oogenesis, nurse cells undergo changes in chromosomal morphology, first from the polytenic form to a transient condensed phase known as the five-blob configuration, then into a diffuse polytenic-polyploid state for the remainder of oogenesis. The mechanism by which nurse-cell chromosome dispersal is regulated remains elusive. Mutations in several genes, including the heterogeneous ribonucleoprotein genes squid (sqd) and hrb27C, the alternative splicing factor gene poly U binding factor 68 kDa (pUf68, also known as half-pint), and the germ-line-specific gene ovarian tumor (otu), that produce defects in nurse-cell chromosome dispersal also produce defects in oocyte polarity, suggesting a link between these two processes. Here, we characterize a novel gene named poly, which, when mutated in the germ line, disrupts nurse-cell chromosome dispersal, as well as localization of anteroposterior and dorsoventral determinants in the oocyte. We also show that poly interacts genetically with hrb27C and otu. We conclude that poly is required for nurse-cell chromosome dispersal and oocyte polarization in the Drosophila germ-line. In addition, our interaction data suggest that poly is probably a member of the characterized mRNP complex that mediates both processes.
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Klusza
authors.forename: Wu-Min
authors.initials: WM
authors.lastname: Deng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D016764:Cell Polarity / Q000235:genetics / Q000502:physiology; D002875:Chromosomes / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005260:Female; D017344:Genes, Insect; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D009030:Mosaicism; D009154:Mutation; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D009866:Oogenesis / Q000235:genetics / Q000502:physiology; D010641:Phenotype; D012261:Ribonucleoproteins / Q000235:genetics / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D016211:Transforming Growth Factor alpha / Q000235:genetics / Q000502:physiology; D014921:Wings, Animal / Q000254:growth & development
chemical_list: D029721:Drosophila Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C487427:Hrb27C protein, Drosophila; D012261:Ribonucleoproteins; D016211:Transforming Growth Factor alpha; C083730:grk protein, Drosophila; C026452:messenger ribonucleoprotein; C061992:otu protein, Drosophila; C552342:poly protein, Drosophila; D049452:Green Fluorescent Proteins
references.citation: Mech Dev. 2002 Dec;119(2):225-37
references.pmid: 12464435
references.citation: Development. 1999 Jan;126(2):293-303
references.pmid: 9847243
references.citation: Genesis. 2007 Jun;45(6):369-81
references.pmid: 17492752
references.citation: Dev Dyn. 2004 Jul;230(3):535-45
references.pmid: 15188438
references.citation: Curr Biol. 2000 Jun 1;10(11):683-6
references.pmid: 10837250
references.citation: Curr Opin Genet Dev. 2001 Aug;11(4):374-83
references.pmid: 11448623
references.citation: Cell. 1993 Oct 8;75(1):165-74
references.pmid: 7691414
references.citation: Genetics. 1997 Dec;147(4):1697-722
references.pmid: 9409831
references.citation: Dev Cell. 2004 May;6(5):625-35
references.pmid: 15130488
references.citation: Genes Dev. 1998 Jun 15;12(12):1847-57
references.pmid: 9637686
references.citation: Cell. 1991 Jul 12;66(1):51-63
references.pmid: 1712672
references.citation: Dev Cell. 2002 Mar;2(3):343-53
references.pmid: 11879639
references.citation: Int J Dev Biol. 2002 Jan;46(1):149-57
references.pmid: 11902676
references.citation: Development. 1992 Aug;115(4):923-36
references.pmid: 1451668
references.citation: Development. 2004 Feb;131(4):775-86
references.pmid: 14724122
references.citation: Dev Dyn. 2006 Jun;235(6):1455-68
references.pmid: 16586443
references.citation: Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12775-80
references.pmid: 16908845
references.citation: Science. 2000 Sep 22;289(5487):2120-2
references.pmid: 11000113
references.citation: Development. 2003 Sep;130(17):4201-15
references.pmid: 12874138
references.citation: Genes Dev. 2006 Apr 15;20(8):977-89
references.pmid: 16598038
references.citation: Science. 1994 Oct 28;266(5185):590-6
references.pmid: 7939713
references.citation: Mech Dev. 1998 Nov;78(1-2):113-8
references.pmid: 9858703
references.citation: Development. 1997 Jan;124(2):461-70
references.pmid: 9053322
references.citation: Dev Biol. 2007 Nov 1;311(1):1-10
references.pmid: 17884037
references.citation: Development. 2001 Dec;128(23):4737-46
references.pmid: 11731454
references.citation: Genes Dev. 2001 Jun 1;15(11):1393-405
references.pmid: 11390359
references.citation: Dev Cell. 2004 Jan;6(1):69-78
references.pmid: 14723848
references.citation: Genetics. 2001 Nov;159(3):1117-34
references.pmid: 11729157
references.citation: Development. 2004 May;131(9):1949-58
references.pmid: 15056611