title: Evidence for the involvement of Lfc and Tctex-1 in axon formation.
abstract: RhoA and Rac play key and opposite roles during neuronal polarization. We now show that Lfc, a guanosine nucleotide exchange factor (GEF), localizes to the Golgi apparatus and growth cones of developing neurons and negatively regulates neurite sprouting and axon formation through a Rho signaling pathway. Tctex-1, a dynein light chain implicated in axon outgrowth by modulating actin dynamics and Rac activity, colocalizes and physically interacts with Lfc, thus inhibiting its GEF activity, decreasing Rho-GTP levels, and functionally antagonizing Lfc during neurite formation.
authors.affiliation: Instituto de Investigación Médica Mercedes y Martín Ferreyra-Consejo Nacional de Investigaciones Científicas y Técnicas, 5016 Cordoba, Argentina.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Conde
authors.forename: Cristina
authors.initials: C
authors.lastname: Arias
authors.forename: Maria
authors.initials: M
authors.lastname: Robin
authors.forename: Aiqun
authors.initials: A
authors.lastname: Li
authors.forename: Masaki
authors.initials: M
authors.lastname: Saito
authors.forename: Jen-Zen
authors.initials: JZ
authors.lastname: Chuang
authors.forename: Angus C
authors.initials: AC
authors.lastname: Nairn
authors.forename: Ching-Hwa
authors.initials: CH
authors.lastname: Sung
authors.forename: Alfredo
authors.initials: A
authors.lastname: Cáceres
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D016466:CHO Cells; D049108:Cell Enlargement; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004398:Dyneins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D020439:Growth Cones / Q000502:physiology; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000502:physiology; D051379:Mice; D016501:Neurites / Q000502:physiology; D051381:Rats; D015398:Signal Transduction; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: C533388:Dynlt1 protein, rat; D020662:Guanine Nucleotide Exchange Factors; C549918:Lfc protein, rat; D004398:Dyneins; D020742:rhoA GTP-Binding Protein
references.citation: Nat Cell Biol. 2005 Mar;7(3):270-7
references.pmid: 15723051
references.citation: Neuron. 2000 May;26(2):431-41
references.pmid: 10839361
references.citation: Brain Res Mol Brain Res. 2005 Oct 3;139(2):277-87
references.pmid: 16024133
references.citation: Nat Neurosci. 2006 Aug;9(8):993-5
references.pmid: 16845384
references.citation: Dev Cell. 2008 Jul;15(1):163-9
references.pmid: 18606149
references.citation: Neuron. 1997 Dec;19(6):1175-86
references.pmid: 9427242
references.citation: J Neurosci. 2008 Sep 10;28(37):9297-308
references.pmid: 18784310
references.citation: J Neurosci. 2007 Aug 29;27(35):9458-68
references.pmid: 17728459
references.citation: Mol Biol Cell. 2004 Jul;15(7):3433-49
references.pmid: 15090620
references.citation: Dev Cell. 2005 Jul;9(1):75-86
references.pmid: 15992542
references.citation: J Biol Chem. 1995 Jul 28;270(30):17656-9
references.pmid: 7629060
references.citation: Dev Cell. 2005 May;8(5):777-86
references.pmid: 15866167
references.citation: Neuron. 2003 Dec 4;40(5):931-44
references.pmid: 14659092
references.citation: Mol Cell Neurosci. 2003 Mar;22(3):405-16
references.pmid: 12691741
references.citation: Trends Cell Biol. 2008 May;18(5):210-9
references.pmid: 18394899
references.citation: J Neurosci. 2001 Apr 1;21(7):2361-72
references.pmid: 11264310
references.citation: Science. 1999 Mar 19;283(5409):1931-4
references.pmid: 10082468
references.citation: Cell. 2003 Jan 10;112(1):63-75
references.pmid: 12526794
references.citation: Neuron. 2006 Sep 21;51(6):727-39
references.pmid: 16982419
references.citation: Dev Cell. 2008 Feb;14(2):205-15
references.pmid: 18267089
references.citation: Nature. 1997 Dec 11;390(6660):632-6
references.pmid: 9403696
references.citation: J Cell Biol. 2003 Sep 29;162(7):1267-79
references.pmid: 14517206
references.citation: Nat Neurosci. 2009 Jun;12(6):735-44
references.pmid: 19448628
references.citation: Nat Rev Neurosci. 2007 Mar;8(3):194-205
references.pmid: 17311006
references.citation: Annu Rev Neurosci. 2009;32:347-81
references.pmid: 19400726
references.citation: Nat Rev Neurosci. 2009 May;10(5):319-32
references.pmid: 19377501
references.citation: J Neurosci. 2001 Aug 1;21(15):5501-12
references.pmid: 11466421
references.citation: Methods Enzymol. 2008;439:285-302
references.pmid: 18374172
references.citation: Dev Cell. 2008 Feb;14(2):150-2
references.pmid: 18267081
references.citation: Cell. 1999 Jun 25;97(7):877-87
references.pmid: 10399916
references.citation: J Biol Chem. 1998 Jul 31;273(31):19639-49
references.pmid: 9677391
references.citation: Cancer Cell. 2002 Mar;1(2):181-91
references.pmid: 12086876
references.citation: Nat Neurosci. 2004 Sep;7(9):923-9
references.pmid: 15286792
references.citation: Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3549-54
references.pmid: 19208802
references.citation: J Neurobiol. 2004 Feb 5;58(2):189-200
references.pmid: 14704951
references.citation: J Biol Chem. 2009 Apr 3;284(14):9489-97
references.pmid: 19158085
references.citation: Neuron. 2005 Jul 7;47(1):85-100
references.pmid: 15996550
references.citation: Genes Dev. 2005 Jan 1;19(1):1-49
references.pmid: 15630019
references.citation: Dev Cell. 2008 Feb;14(2):216-26
references.pmid: 18267090
references.citation: Mol Cell Biol. 2009 Nov;29(21):5963-73
references.pmid: 19667072
references.citation: Toxicol Sci. 2008 Aug;104(2):352-61
references.pmid: 18480073