Isolation and characterization of Bacteroides host strain HB-73 used to detect sewage specific phages in Hawaii.
Previous studies have shown that Escherichia coli and enterococci are unreliable indicators of fecal contamination in Hawaii because of their ability to multiply in environmental soils. In this study, the method of detecting Bacteroides phages as specific markers of sewage contamination in Hawaii's recreational waters was evaluated because these sewage specific phages cannot multiply under environmental conditions. Bacteroides hosts (GB-124, GA-17), were recovered from sewage samples in Europe and were reported to be effective in detecting phages from sewage samples obtained in certain geographical areas. However, GB-124 and GA-17 hosts were ineffective in detecting phages from sewage samples obtained in Hawaii. Bacteroides host HB-73 was isolated from a sewage sample in Hawaii, confirmed as a Bacteroides sp. and shown to recover phages from multiple sources of sewage produced in Hawaii at high concentrations (5.2-7.3 x 10(5) PFU/100 mL). These Bacteroides phages were considered as potential markers of sewage because they also survived for three days in fresh stream water and two days in marine water. Water samples from Hawaii's coastal swimming beaches and harbors, which were known to be contaminated with discharges from streams, were shown to contain moderate (20-187 CFU/100 mL) to elevated (173-816 CFU/100 mL) concentrations of enterococci. These same samples contained undetectable levels (<10 PFU/100 mL) of F+ coliphage and Bacteroides phages and provided evidence to suggest that these enterococci may not necessarily be associated with the presence of raw sewage. These results support previous conclusions that discharges from streams are the major sources of enterococci in coastal waters of Hawaii and the most likely source of these enterococci is from environmental soil rather than from sewage.
Vijayavel
Kannappan
K
Water Resources Research Center, University of Hawaii, 2540 Dole Street, Honolulu, HI 96822, USA.
Fujioka
Roger
R
Ebdon
James
J
Taylor
Huw
H
D001435:Bacteriophages / Q000302:isolation & purification*; D001439:Bacteroides / Q000302:isolation & purification* / Q000821:virology*; D005243:Feces / Q000382:microbiology; D005618:Fresh Water / Q000382:microbiology; D005843:Geography; D006254:Hawaii; D050296:Microbial Viability; D009391:Nephelometry and Turbidimetry; D011998:Recreation; D054712:Salinity; D012623:Seawater / Q000382:microbiology; D012722:Sewage / Q000821:virology*; D013550:Swimming; D014871:Water Microbiology; D014876:Water Pollution / Q000032:analysis
D012722:Sewage