title: Dynamic flexibility of DNA repair pathways in growth arrested Escherichia coli.
abstract: The DNA of all organisms is constantly damaged by exogenous and endogenous agents. Base excision repair (BER) is important for the removal of several non-bulky lesions from the DNA, however not much is known about the contributions of other DNA repair pathways to the processing of non-bulky lesions. Here we utilized a luciferase reporter system to assess the contributions of transcription-coupled repair (TCR), BER and nucleotide excision repair (NER) to the repair of two non-bulky lesions, 8-oxoguanine (8OG) and uracil (U), in vivo under non-growth conditions. We demonstrate that both TCR and NER are utilized by Escherichia coli to repair 8OG and U. Additionally, the relative level of recognition of these lesions by BER and NER suggests that TCR can utilize components of either pathway for lesion removal, depending upon their availability. These findings indicate a dynamic flexibility of DNA repair pathways in the removal of non-bulky DNA lesions in prokaryotes, and reveal their respective contributions to the repair of 8OG and U in vivo.
authors.affiliation: Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Clauson
authors.forename: Tina T
authors.initials: TT
authors.lastname: Saxowsky
authors.forename: Paul W
authors.initials: PW
authors.lastname: Doetsch
mesh_terms: D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development*; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D008957:Models, Genetic; D014158:Transcription, Genetic; D014498:Uracil / Q000378:metabolism
chemical_list: C024829:8-hydroxyguanine; D014498:Uracil; D006147:Guanine
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