title: Constructing an improved pyrroloquinoline quinone glucose dehydrogenase binding aptamer for enzyme labeling.
abstract: Motif analysis of PGa4 indicated that eight consecutive guanine residues were most important for pyrroloquinoline quinine glucose dehydrogenase (PQQGDH) recognition. Because the truncated mutants minimized unexpected interactions between PGa4 and the target-binding aptamer, they will now facilitate the construction of a highly sensitive detection system using a PQQGDH-labeled diagnostic marker-binding aptamer.
authors.affiliation: Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Abe
authors.forename: Koji
authors.initials: K
authors.lastname: Sode
authors.forename: Kazunori
authors.initials: K
authors.lastname: Ikebukuro
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism*; D005948:Glucose Dehydrogenases / Q000378:metabolism*; D011485:Protein Binding; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D005948:Glucose Dehydrogenases; C033955:glucose dehydrogenase (pyrroloquinoline-quinone)