An automated two-phase microfluidic system for kinetic analyses and the screening of compound libraries.
Droplet-based microfluidic systems allow biological and chemical reactions to be performed on a drastically decreased scale. However, interfacing the outside world with such systems and generating high numbers of microdroplets of distinct chemical composition remain challenging. We describe here an automated system in which arrays of chemically distinct plugs are generated from microtiter plates. Each array can be split into multiple small-volume copies, thus allowing several screens of the same library. The system is fully compatible with further on-chip manipulation(s) and allows monitoring of individual plugs over time (e.g. for recording reaction kinetics). Hence the technology eliminates several bottlenecks of current droplet-based microfluidic systems and should open the way for (bio-)chemical and cell-based screens.
Clausell-Tormos
Jenifer
J
Institut de Science et d'Ingénierie Supramoléculaires (ISIS), Université de Strasbourg, CNRS UMR 7006, 8, allée Gaspard Monge, BP 70028, 67083, Strasbourg Cedex, France.
Griffiths
Andrew D
AD
Merten
Christoph A
CA
D001331:Automation; D003374:Coumarins / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007700:Kinetics; D046210:Microfluidic Analytical Techniques; D001616:beta-Galactosidase / Q000037:antagonists & inhibitors / Q000378:metabolism
D003374:Coumarins; D004791:Enzyme Inhibitors; D001616:beta-Galactosidase; C080488:7-aminocoumarin