title: Effects of hot-water extract of Toona sinensis on immune response and resistance to Aeromonas hydrophila in Oreochromis mossambicus.
abstract: Respiratory burst, lysozyme and phagocytic activities, and immunoglobulin levels in response to the pathogen Aeromonas hydrophila were examined in tilapia (Oreochromis mossanbicus, 10.65 +/- 2.5 g) injected individually with hot-water extract of Toona sinensis at 4 or 8 microg g(-1). Tilapia receiving the hot-water extract of T. sinensis at either dose had significantly increased respiratory burst, phagocytic activity and lysozyme activity towards A. hydrophila by 1 and 2 days post injection. No significant differences in total immunoglobulin levels were observed among the tilapia that received the two different doses of hot-water extract of T. sinensis at 4 and 8 microg g(-1). In another experiment, a Tilapia that had been injected with hot-water extract of T. sinensis was challenged with A. hydrophila at 5 x 10(7) colony-forming units (cfu) fish(-1). The survival of tilapia that received the hot-water extract of T. sinensis at 8 microg g(-1) was significantly higher than fish that received phosphate buffered saline and the control fish after 2 days, and at the termination of the experiment (7 days after the challenge). It was concluded that the hot-water extract of T. sinensis at 8 microg g(-1) or less had increased the immune response and resistance to A. hydrophila infection in tilapia.
authors.affiliation: Department of Nutrition and Health Science, Chang Jung Christian University, Tainan 711, Taiwan.
authors.forename: Chih-Chung
authors.initials: CC
authors.lastname: Wu
authors.forename: Chun-Hung
authors.initials: CH
authors.lastname: Liu
authors.forename: Yueh-Ping
authors.initials: YP
authors.lastname: Chang
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Hsieh
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D016980:Aeromonas hydrophila; D000818:Animals; D005393:Fish Diseases / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000662:veterinary*; D007113:Immunity, Innate / Q000187:drug effects*; D029582:Meliaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D017210:Tilapia / Q000276:immunology / Q000502:physiology*; D014867:Water / Q000737:chemistry
chemical_list: D000276:Adjuvants, Immunologic; D010936:Plant Extracts; D014867:Water