title: Microbial production of 2,3-butanediol by a surfactant (serrawettin)-deficient mutant of Serratia marcescens H30.
abstract: Serrawettin W1 produced by Serratia marcescens is a surface-active exolipid resulting in a lot foam formation during the 2,3-butanediol (2,3-BD) fermentation process. In order to avoid excessive addition of antifoam agent and microbial contamination, S. marcescens mutants deficient in serrawettin W1 formation were successfully constructed through insertional inactivation of the swrW gene coding for serrawettin W1 synthase. The shake flask and batch experiments suggested that disruption of the swrW gene led to significant reduction of the foam formation and improved 2,3-BD production a little. Ultimately, fed-batch culturing of the mutant afforded a maximum 2,3-BD concentration of 152 g l(-1) with a productivity of 2.67 g l(-1) h(-1) and a yield of 92.6% at 57 h.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
authors.forename: Liaoyuan
authors.initials: L
authors.lastname: Zhang
authors.forename: Jian'an
authors.initials: J
authors.lastname: Sun
authors.forename: Yingli
authors.initials: Y
authors.lastname: Hao
authors.forename: Jiawen
authors.initials: J
authors.lastname: Zhu
authors.forename: Ju
authors.initials: J
authors.lastname: Chu
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
authors.forename: Yaling
authors.initials: Y
authors.lastname: Shen
mesh_terms: D002072:Butylene Glycols / Q000378:metabolism*; D047630:Depsipeptides / Q000172:deficiency*; D055786:Gene Knockout Techniques; D016254:Mutagenesis, Insertional; D012706:Serratia marcescens / Q000235:genetics* / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; D047630:Depsipeptides; C026978:2,3-butylene glycol; C009246:serratamolide
references.citation: Biotechnol Bioeng. 1988 Mar;31(4):366-77
references.pmid: 18584617
references.citation: Appl Microbiol Biotechnol. 2010 Feb;85(6):1751-8
references.pmid: 19756578
references.citation: Arch Mikrobiol. 1958;32(1):16-9
references.pmid: 13617968
references.citation: J Bacteriol. 1998 Dec;180(23):6384-8
references.pmid: 9829950
references.citation: Appl Environ Microbiol. 1983 Sep;46(3):630-5
references.pmid: 6357080
references.citation: Microbiol Immunol. 2005;49(4):303-10
references.pmid: 15840955
references.citation: Appl Microbiol Biotechnol. 2008 Apr;78(6):917-26
references.pmid: 18320188
references.citation: Biotechnol Adv. 2009 Nov-Dec;27(6):715-725
references.pmid: 19442714
references.citation: J Bacteriol. 1990 Nov;172(11):6568-72
references.pmid: 2172217
references.citation: J Bacteriol. 2006 Jun;188(12):4570-2
references.pmid: 16740963
references.citation: J Bacteriol. 1992 Mar;174(6):1769-76
references.pmid: 1548227
references.citation: Bioresour Technol. 2010 Mar;101(6):1961-7
references.pmid: 19932023
references.citation: FEMS Microbiol Lett. 1989 Oct 15;52(3):243-6
references.pmid: 2693193