title: Global transcription engineering of brewer's yeast enhances the fermentation performance under high-gravity conditions.
abstract: Global transcription engineering was developed as a tool to reprogram gene transcription for eliciting new phenotypes important for technological applications (Science 2006, 314(5805):1565-1568). A recent report indicated that the beneficial growth advantage of yeast cells expressing the SPT15-300 mutation is the result of enhanced uptake and/or improved utilization of leucine and thus was seen only on defined media with low concentrations of leucine (Appl Environ Microbiol 2009, 75(19):6055-6061). Further investigation towards a leucine-prototrophic strain of industrial lager brewer's yeast indicated that integration one copy of SPT15-300 in SPT15 allele, however, did lead to an increased ethanol tolerance on complex rich medium at high gravity fermentation condition. Under brewing conditions, the SPT15-300 mutant produced 80.78 g/L ethanol from 200 g/L carbohydrates after 384 h, almost twice as much as that of the wild-type strain. The results convinced us that the effect of global regulator modification of yeast is at multi-genes level and is extremely complicated.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Cuijuan
authors.initials: C
authors.lastname: Gao
authors.forename: Zhikun
authors.initials: Z
authors.lastname: Wang
authors.forename: Quanfeng
authors.initials: Q
authors.lastname: Liang
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Qi
mesh_terms: D000431:Ethanol / Q000378:metabolism / Q000633:toxicity; D005285:Fermentation; D005818:Genetic Engineering; D018471:Hypergravity; D050505:Mutant Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics; D035181:TATA-Box Binding Protein / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D050505:Mutant Proteins; C115017:SPT15 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; D000431:Ethanol