title: Quantitative analysis of creatinine in urine by metalized nanostructured parylene.
abstract: A highly accurate, real-time multisensor agent monitor for biomarker detection is required for early detection of kidney diseases. Urine creatinine level can provide useful information on the status of the kidney. We prepare nanostructured surface-enhanced Raman spectroscopy (SERS) substrates without template or lithography, which provides controllable, well-organized nanostructures on the surface, for the quantitative analysis of creatinine concentration in urine. We present our work on sensitivity of the SERS substrate to urine samples collected from diabetic patients and healthy persons. We report the preparation of a new type of SERS substrate, which provides fast (<10 s), highly sensitive (creatinine concentration <0.5 microg/mL) and reproducible (<5% variation) detection of urine. Our method to analyze the creatinine level in urine is in good agreement with the enzymatic method.
authors.affiliation: Pennsylvania State University, Department of Engineering Science, University Park, Pennsylvania 16802, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Niranjan
authors.initials: N
authors.lastname: Malvadkar
authors.forename: S
authors.initials: S
authors.lastname: Koytek
authors.forename: J
authors.initials: J
authors.lastname: Bylander
authors.forename: W Brian
authors.initials: WB
authors.lastname: Reeves
authors.forename: Melik C
authors.initials: MC
authors.lastname: Demirel
mesh_terms: D003404:Creatinine / Q000652:urine*; D006801:Humans; D008670:Metals / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*; D016482:Urinalysis / Q000379:methods*; D014992:Xylenes / Q000737:chemistry*
chemical_list: D008670:Metals; D011108:Polymers; D014992:Xylenes; C011055:parylene; D003404:Creatinine