FTIR study on the light sensitivity of the [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F: Ni-C to Ni-L photoconversion, kinetics of proton rebinding and H/D isotope effect.
The light-induced Ni-C to Ni-L transition results in the dissociation of a hydrogenic species, originating from the dihydrogen splitting at the active site. Back conversion in the dark to form Ni-C was investigated by studying the rebinding kinetics of this ligand in protonated (H(2)/H(2)O) and deuterated (D(2)/D(2)O) samples using time resolved FTIR spectroscopy.
Kellers
Petra
P
Max-Planck-Institut für Bioanorganische Chemie, Stiftstrasse 34-36, D-45470 Mülheim an der Ruhr, Germany.
Pandelia
Maria-Eirini
ME
Currell
Leslie J
LJ
Görner
Helmut
H
Lubitz
Wolfgang
W
D020134:Catalytic Domain; D016969:Desulfovibrio vulgaris / Q000201:enzymology*; D003903:Deuterium / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D006864:Hydrogenase / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008027:Light; D008958:Models, Molecular; D010777:Photochemistry; D011522:Protons; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014867:Water / Q000737:chemistry
D011522:Protons; D014867:Water; D006859:Hydrogen; D003903:Deuterium; C043296:nickel-iron hydrogenase; D006864:Hydrogenase