title: Integrity and biological activity of DNA after UV exposure.
abstract: The field of astrobiology lacks a universal marker with which to indicate the presence of life. This study supports the proposal to use nucleic acids, specifically DNA, as a signature of life (biosignature). In addition to its specificity to living organisms, DNA is a functional molecule that can confer new activities and characteristics to other organisms, following the molecular biology dogma, that is, DNA is transcribed to RNA, which is translated into proteins. Previous criticisms of the use of DNA as a biosignature have asserted that DNA molecules would be destroyed by UV radiation in space. To address this concern, DNA in plasmid form was deposited onto different surfaces and exposed to UVC radiation. The surviving DNA was quantified via the quantitative polymerase chain reaction (qPCR). Results demonstrate increased survivability of DNA attached to surfaces versus non-adsorbed DNA. The DNA was also tested for biological activity via transformation into the bacterium Acinetobacter sp. and assaying for antibiotic resistance conferred by genes encoded by the plasmid. The success of these methods to detect DNA and its gene products after UV exposure (254 nm, 3.5 J/m(2)s) not only supports the use of the DNA molecule as a biosignature on mineral surfaces but also demonstrates that the DNA retained biological activity.
authors.affiliation: Environmental Microbial Genomics Group, Laboratoire Ampère, Ecole Centrale de Lyon, Université de Lyon, Ecully, France.
authors.forename: Delina Y
authors.initials: DY
authors.lastname: Lyon
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Monier
authors.forename: Sébastien
authors.initials: S
authors.lastname: Dupraz
authors.forename: Caroline
authors.initials: C
authors.lastname: Freissinet
authors.forename: Pascal
authors.initials: P
authors.lastname: Simonet
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Vogel
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry; D000077215:Clay; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects*; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic; D014466:Ultraviolet Rays
chemical_list: D000538:Aluminum Silicates; D004247:DNA; D000077215:Clay