title: Filled and glycosylated carbon nanotubes for in vivo radioemitter localization and imaging.
abstract: Functionalization of nanomaterials for precise biomedical function is an emerging trend in nanotechnology. Carbon nanotubes are attractive as multifunctional carrier systems because payload can be encapsulated in internal space whilst outer surfaces can be chemically modified. Yet, despite potential as drug delivery systems and radiotracers, such filled-and-functionalized carbon nanotubes have not been previously investigated in vivo. Here we report covalent functionalization of radionuclide-filled single-walled carbon nanotubes and their use as radioprobes. Metal halides, including Na(125)I, were sealed inside single-walled carbon nanotubes to create high-density radioemitting crystals and then surfaces of these filled-sealed nanotubes were covalently modified with biantennary carbohydrates, improving dispersibility and biocompatibility. Intravenous administration of Na(125)I-filled glyco-single-walled carbon nanotubes in mice was tracked in vivo using single-photon emission computed tomography. Specific tissue accumulation (here lung) coupled with high in vivo stability prevented leakage of radionuclide to high-affinity organs (thyroid/stomach) or excretion, and resulted in ultrasensitive imaging and delivery of unprecedented radiodose density. Nanoencapsulation of iodide within single-walled carbon nanotubes enabled its biodistribution to be completely redirected from tissue with innate affinity (thyroid) to lung. Surface functionalization of (125)I-filled single-walled carbon nanotubes offers versatility towards modulation of biodistribution of these radioemitting crystals in a manner determined by the capsule that delivers them. We envisage that organ-specific therapeutics and diagnostics can be developed on the basis of the nanocapsule model described here.
authors.affiliation: Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, UK.
authors.forename: Sung You
authors.initials: SY
authors.lastname: Hong
authors.forename: Gerard
authors.initials: G
authors.lastname: Tobias
authors.forename: Khuloud T
authors.initials: KT
authors.lastname: Al-Jamal
authors.forename: Belén
authors.initials: B
authors.lastname: Ballesteros
authors.forename: Hanene
authors.initials: H
authors.lastname: Ali-Boucetta
authors.forename: Sergio
authors.initials: S
authors.lastname: Lozano-Perez
authors.forename: Peter D
authors.initials: PD
authors.lastname: Nellist
authors.forename: Robert B
authors.initials: RB
authors.lastname: Sim
authors.forename: Ciara
authors.initials: C
authors.lastname: Finucane
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Mather
authors.forename: Malcolm L H
authors.initials: ML
authors.lastname: Green
authors.forename: Kostas
authors.initials: K
authors.lastname: Kostarelos
authors.forename: Benjamin G
authors.initials: BG
authors.lastname: Davis
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D050260:Carbohydrate Metabolism; D006031:Glycosylation; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D017348:Microscopy, Electron, Scanning Transmission / Q000379:methods; D036103:Nanotechnology / Q000379:methods / Q000639:trends*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D011868:Radioisotopes / Q000378:metabolism / Q000493:pharmacokinetics; D013270:Stomach / Q000000981:diagnostic imaging; D013961:Thyroid Gland / Q000000981:diagnostic imaging; D014018:Tissue Distribution; D014057:Tomography, X-Ray Computed / Q000379:methods
chemical_list: D037742:Nanotubes, Carbon; D011868:Radioisotopes; D000117:Acetylglucosamine
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