Role of collapsin response mediator protein-2 in neurite outgrowth of PC12 cells.
Collapsin response mediator proteins (CRMPs) are involved in cell differentiation and axonal growth. In this study, we investigated neurite outgrowth and the expression of CRMP-2 in PC12 cells. Nondifferentiated PC12 cells hardly express CRMP-2, but the expression of CRMP-2 increases with neurite outgrowth. We established a stable CRMP-2-overexpressing PC12 cell line and found that PC12 cells, which constitutively overexpress CRMP-2, were unable to extend neurites after stimulation with the nerve growth factor or the neural cell adhesion molecule, a procedure that promotes neurite outgrowth in untransfected cells. In contrast, a PC12 cell line deficient in CRMP-2 extends neurites after the stimulation of nerve growth factor or neural cell adhesion molecule.
Bork
Kaya
K
Institute of Physiological Chemistry, Martin-Luther University of Halle-Wittenberg, Halle/Saale 06114, Germany.
Karbe
Yvonne
Y
Pollscheit
Juliane
J
Glaubitz
Nicole
N
Nöhring
Sabine
S
Horstkorte
Rüdiger
R
D000818:Animals; D015153:Blotting, Western; D002452:Cell Count; D049108:Cell Enlargement; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018920:Coculture Techniques; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D020932:Nerve Growth Factor / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D016501:Neurites / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D014162:Transfection
D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D019006:Neural Cell Adhesion Molecules; C095114:collapsin response mediator protein-2; D020932:Nerve Growth Factor