Chemical analysis of Agaricus blazei polysaccharides and effect of the polysaccharides on IL-1beta mRNA expression in skin of burn wound-treated rats.
Agaricus blazei polysaccharides were analyzed by GC-MS. Results indicated that the polysaccharides contained glucose (93.87%), mannose (3.54%), and arabinose (2.25%). The compositional analysis was completed by the methylation data. These data indicated that Agaricus blazei polysaccharides are glucans. Compared to model rats, rats fed with Agaricus blazei polysaccharides showed a decrease of ratio of IL-1beta/beta-actin and IL-1beta level in skin of burn wound. Recovery rate of wound skin increased with increasing dose of polysaccharides. The results indicated that Agaricus blazei polysaccharides could be useful in promote burn wound healing.
Sui
ZhiFu
Z
Aesthetic and Plastic Surgery Center, Dermatology Diagnosis and Treatment Center of PLA, General Hospital of Beijing Military Region, Beijing 100125, China.
Yang
RongYa
R
Liu
Biao
B
Gu
TingMin
T
Zhao
Zhili
Z
Shi
Dongfang
D
Chang
DongQing
D
D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D002056:Burns / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D053583:Interleukin-1beta / Q000235:genetics*; D011134:Polysaccharides / Q000032:analysis* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects / Q000293:injuries / Q000378:metabolism*
D053583:Interleukin-1beta; D011134:Polysaccharides; D012333:RNA, Messenger