PCR based rapid detection of Salmonella from poultry samples.
Salmonella is very important from the zoonotic point of view, as it causes many diseases in animals and humans. This study was conducted during September 2005 to February 2006 to develop rapid detection system for Salmonella from poultry samples. In the present study 300 poultry samples were screened for Salmonella. Earlier, isolation and identification of Salmonella from clinical samples by traditional cultural techniques required laborious procedures which can last upto 7 days, whereas amplification of DNA sequences unique to an organism using the PCR improves both the speed of detection and the level of sensitivity at which organisms can be detected and has been increasingly used to identify several bacterial species from food and clinical samples. In this study Salmonella were rapidly detected by targeting invA gene, giving PCR product of 284 bp size. Therefore this technique can be used for the screening of Salmonella in the routine testing.
Siddique
R A
RA
Veterinary Biochemistry, C.V.Sc. & A.H., NavsariAgricultural University, Gujarat, India. riazndri@gmail.com
Saxena
M
M
Lakhchaura
B D
BD
D000818:Animals; D002645:Chickens / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000382:microbiology*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*