title: Characterisation of a novel cell line (CSQT-2) with high metastatic activity derived from portal vein tumour thrombus of hepatocellular carcinoma.
abstract: BACKGROUND
  Portal vein tumour thrombus (PVTT) is highly associated with the progression and metastasis of hepatocellular carcinoma (HCC). However, there are no appropriate cell models of PVTT with which to study the biological and physiological characteristics of PVTT.
  
  
  METHODS
  Primary cell culture was performed by the use of a successive xenograft line called PVTT-#1, which was obtained from a 60-year-old male HCC patient accompanied by PVTT.
  
  
  RESULTS
  A successive cell line named CSQT-2 was established. The cell line showed aggressive phenotypes in terms of cell growth, survival, migration, xenograft and metastasis. Moreover, an orthotopic transplantation assay showed that PVTT can be generated in nude mice when CSQT-2 cells were inoculated in the liver and that it shows a typical migratory tendency in the vascular branches of portal vein. Moreover, the established CSQT-2 cells also showed varied expression of tumour-initiating cell (TIC) markers such as CD133, CD90 and EpCAM.
  
  
  CONCLUSIONS
  Establishment of CSQT-2 may provide a suitable model with which to investigate the molecular mechanisms of PVTT-related HCC.
authors.affiliation: Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China.
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.forename: H S
authors.initials: HS
authors.lastname: Hu
authors.forename: Y X
authors.initials: YX
authors.lastname: Feng
authors.forename: J
authors.initials: J
authors.lastname: Shi
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.forename: W X
authors.initials: WX
authors.lastname: Guo
authors.forename: J
authors.initials: J
authors.lastname: Xue
authors.forename: D
authors.initials: D
authors.lastname: Xie
authors.forename: S R
authors.initials: SR
authors.lastname: Liu
authors.forename: M C
authors.initials: MC
authors.lastname: Wu
authors.forename: S Q
authors.initials: SQ
authors.lastname: Cheng
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000150:complications / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor / Q000473:pathology*; D002465:Cell Movement / Q000502:physiology; D020732:Cytogenetic Analysis; D006801:Humans; D008113:Liver Neoplasms / Q000150:complications / Q000235:genetics / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009362:Neoplasm Metastasis; D014411:Neoplastic Stem Cells / Q000473:pathology; D011169:Portal Vein / Q000473:pathology*; D013927:Thrombosis / Q000150:complications / Q000209:etiology / Q000473:pathology*; D014183:Transplantation, Heterologous; D019043:Vascular Neoplasms / Q000150:complications / Q000235:genetics / Q000473:pathology / Q000556:secondary*
references.citation: Lancet. 1971 Oct 30;2(7731):971-2
references.pmid: 4107917
references.citation: Hepatology. 2008 Mar;47(3):919-28
references.pmid: 18275073
references.citation: World J Gastroenterol. 2009 Oct 21;15(39):4896-906
references.pmid: 19842219
references.citation: Surgery. 2009 Jan;145(1):9-19
references.pmid: 19081470
references.citation: Hepatogastroenterology. 2006 Mar-Apr;53(68):249-52
references.pmid: 16608033
references.citation: Curr Stem Cell Res Ther. 2009 May;4(2):147-53
references.pmid: 19442199
references.citation: Br J Cancer. 2007 Jul 2;97(1):50-7
references.pmid: 17551499
references.citation: Ann Surg Oncol. 2010 Jan;17(1):179-85
references.pmid: 19727956
references.citation: Int J Cancer. 2010 May 1;126(9):2067-78
references.pmid: 19711346
references.citation: Ann Surg Oncol. 2009 May;16(5):1254
references.pmid: 19277788
references.citation: J Nippon Med Sch. 2009 Aug;76(4):217-20
references.pmid: 19755798
references.citation: Curr Pharm Biotechnol. 2009 Feb;10(2):192-6
references.pmid: 19199951
references.citation: Cancer Cell. 2008 Feb;13(2):153-66
references.pmid: 18242515
references.citation: J Gastroenterol Hepatol. 2006 Oct;21(10):1561-6
references.pmid: 16928217
references.citation: J Hepatobiliary Pancreat Surg. 2009;16(5):688-91
references.pmid: 19290461
references.citation: Surg Clin North Am. 2009 Feb;89(1):97-113, ix
references.pmid: 19186233
references.citation: Br J Cancer. 1999 Nov;81(5):814-21
references.pmid: 10555751
references.citation: Dig Dis Sci. 2007 Nov;52(11):3290-5
references.pmid: 17394062
references.citation: Gastroenterology. 2009 Mar;136(3):1012-24
references.pmid: 19150350
references.citation: Surg Oncol Clin N Am. 2003 Jan;12(1):25-34, viii
references.pmid: 12735127
references.citation: J Cancer Res Clin Oncol. 2008 Mar;134(3):299-305
references.pmid: 17828420
references.citation: Hepatogastroenterology. 2007 Mar;54(74):499-502
references.pmid: 17523307
references.citation: Ann Surg. 2006 Feb;243(2):229-35
references.pmid: 16432356
references.citation: J Surg Res. 2010 Mar;159(1):538-44
references.pmid: 19111323
references.citation: Gastroenterology. 2008 Dec;135(6):2128-40, 2140.e1-8
references.pmid: 18801366
references.citation: Methods Enzymol. 1979;58:141-52
references.pmid: 423756
references.citation: J Cancer Res Clin Oncol. 2004 Apr;130(4):187-96
references.pmid: 14685850
references.citation: J Hepatobiliary Pancreat Surg. 1998;5(2):212-6
references.pmid: 9745091
references.citation: World J Surg Oncol. 2005 May 20;3:27
references.pmid: 15907199
references.citation: World J Surg. 2008 Apr;32(4):627-31
references.pmid: 18228094
references.citation: Int J Cancer. 1996 Apr 10;66(2):239-43
references.pmid: 8603818
references.citation: BMC Cancer. 2008 Nov 27;8:351
references.pmid: 19038025
references.citation: Prostate. 2009 Jun 1;69(8):827-37
references.pmid: 19204913
references.citation: J Cancer Res Clin Oncol. 2009 May;135(5):715-22
references.pmid: 19002496
references.citation: Clin Liver Dis. 2005 May;9(2):191-211, v
references.pmid: 15831268
references.citation: J Clin Invest. 1986 Aug;78(2):424-30
references.pmid: 3461004
references.citation: Hepatogastroenterology. 2004 Sep-Oct;51(59):1445-7
references.pmid: 15362772
references.citation: Langenbecks Arch Surg. 2005 Jun;390(3):259-65
references.pmid: 15875211
references.citation: Int J Cancer. 2006 Jun 15;118(12):3030-44
references.pmid: 16404738
references.citation: Indian J Pathol Microbiol. 2008 Jul-Sep;51(3):323-8
references.pmid: 18723951
references.citation: BMC Cancer. 2009 Sep 11;9:324
references.pmid: 19744348
references.citation: Cancer Res. 2008 Mar 1;68(5):1451-61
references.pmid: 18316609
references.citation: Liver Int. 2008 Apr;28(4):509-18
references.pmid: 18339078
references.citation: Toxicol Sci. 2008 May;103(1):4-13
references.pmid: 17890765