The role of heat shock protein 27 in bronchiolitis obliterans syndrome after lung transplantation.
BACKGROUND
Heat shock proteins (Hsps) are a family of evolutionary conserved proteins classified according to their size as small and large Hsps. They have a cytoprotective role and have been shown to be immunogenic molecules. In addition, self-reactivity to Hsps has been implicated in various autoimmune diseases and in the development of alloimmunity. This study examined the relationship of large and small Hsps and anti-Hsp antibodies in lung transplant (LTx) recipients who have bronchiolitis obliterans syndrome (BOS).


METHODS
Anti-Hsp27 and Hsp70 antibodies, and Hsp27, Hsp60, and Hsp70 protein expression levels were evaluated in serum and bronchoalveolar lavage samples collected from 8 LTx recipients with established BOS and 8 recipients without BOS (controls). Serum from 8 healthy individuals was examined for Hsp levels as a comparison.


RESULTS
Elevated serum Hsp27 levels were observed in recipients with BOS compared with controls or healthy individuals, whereas Hsp70 and Hsp60 expression showed no difference. Anti-Hsp27 antibody levels were significantly higher in the BAL of recipients with BOS than in those without BOS. In contrast, anti-Hsp70 antibodies levels in serum or BAL showed no difference between groups.


CONCLUSIONS
These results support the novel concept that Hsp27, but not the classic Hsp60 and Hsp70, may be associated with the development BOS. The expression of anti-Hsp27 antibodies found only in the BAL fluid suggests a local response occurring at the level of the alveoli and terminal airways.
Wood
Karen L
KL
The Ohio State University College of Medicine, Columbus, OH, USA. karen.wood@osumc.edu
Nunley
David R
DR
Moffatt-Bruce
Susan
S
Pope-Harman
Amy
A
Huang
Qin
Q
Shamo
Eric N
EN
Phillips
Gary S
GS
Baran
Chris
C
Batra
Sanjay
S
Marsh
Clay B
CB
Doseff
Andrea I
AI
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000378:metabolism; D001989:Bronchiolitis Obliterans / Q000276:immunology / Q000503:physiopathology*; D001992:Bronchoalveolar Lavage Fluid; D016022:Case-Control Studies; D018834:Chaperonin 60 / Q000276:immunology / Q000378:metabolism; D005260:Female; D006084:Graft Rejection / Q000276:immunology / Q000503:physiopathology; D055551:HSP27 Heat-Shock Proteins / Q000276:immunology / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016040:Lung Transplantation / Q000276:immunology / Q000502:physiology*; D008297:Male
D000888:Antibodies, Anti-Idiotypic; D018834:Chaperonin 60; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins
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