[Preparative isolation and purification of ergosterol from a strain of Paecilomyces hepialid by high-speed counter-current chromatography].
To develop an effective and rapid method for the preparation of ergosterol from a strain of Paecilomyces hepialid, the ethyl acetate extract of the mycelia was injected into a high-speed counter-current chromatograph (HSCCC) directly, and eluted with different solvent systems. The result showed that the solvent system composed of n-hexane-ethyl acetate-methanol-water (6:1.7:6:0.3, v/v/v/v) was the best. The lower phase was used as the mobile phase and performed at a flow rate of 2 mL/min, while the apparatus rotated at 850 r/min, and detected at 280 nm. The prepared ergosterol was identified with ultraviolet detection (UV), high resolution mass spectrometer (HRMS) and standard, and its purity was 99.2% analyzed by high performance liquid chromatography. The established method is relatively simple, fast, and suitable for the large-scale isolation and separation of ergosterol.
Zhang
Nengsheng
N
Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei 230036, China.
Wang
Jinbin
J
Wang
Xiaoyan
X
Wang
Xiaodong
X
Hu
Fenglin
F
D000818:Animals; D003377:Countercurrent Distribution / Q000379:methods*; D004875:Ergosterol / Q000096:biosynthesis / Q000302:isolation & purification*; D010143:Paecilomyces / Q000737:chemistry*
D004875:Ergosterol