title: Characterization of low molecular weight chemical fractions of dry bean (Phaseolus vulgaris) for bioactivity using Caenorhabditis elegans longevity and metabolite fingerprinting.
abstract: Dry bean consumption has been reported to be associated with reduced risk for a number of chronic diseases including cancer. The extent to which these benefits are associated with primary versus secondary plant metabolites is not known. The work reported herein focuses on low molecular weight secondary metabolites and uses longevity extension of wild-type Caenorhabditis elegans nematodes as a surrogate marker for human health benefits. A modified Bligh and Dyer technique was used to extract freeze-dried bean, and the resulting fractions were evaluated for longevity extension and metabolite fingerprinting using ultra performance liquid chromatography-mass spectrometry (UPLC-MS). Dry bean extracts extended adult C. elegans lifespan by as much as 16%. Hydrophilic fractions increased lifespan, whereas the hydrophobic fraction induced longevity reduction. Metabolite fingerprinting revealed distinguishing spectral differences among the four chemical fractions evaluated and demonstrated that within each fraction chemical composition differed significantly based on dry bean genetic heritage.
authors.affiliation: Cancer Prevention Laboratory, Department of Horticulture, Colorado State University, Fort Collins, Colorado 80523, USA.
authors.forename: Meghan M
authors.initials: MM
authors.lastname: Mensack
authors.forename: Vanessa K
authors.initials: VK
authors.lastname: Fitzgerald
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Lewis
authors.forename: Henry J
authors.initials: HJ
authors.lastname: Thompson
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008136:Longevity / Q000187:drug effects*; D013058:Mass Spectrometry; D023421:Models, Animal; D008970:Molecular Weight; D027805:Phaseolus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts
references.citation: Pharmacogenomics. 2007 Jul;8(7):731-41
references.pmid: 18240906
references.citation: J Clin Oncol. 2007 May 10;25(14):1844-51
references.pmid: 17488982
references.citation: Cancer Epidemiol Biomarkers Prev. 2000 Aug;9(8):795-804
references.pmid: 10952096
references.citation: Mech Ageing Dev. 2009 Aug;130(8):477-86
references.pmid: 19501612
references.citation: Free Radic Biol Med. 2009 Feb 1;46(3):414-21
references.pmid: 19061950
references.citation: Aging Cell. 2008 Jun;7(3):305-17
references.pmid: 18248662
references.citation: J Cell Biochem. 2008 May 1;104(1):339-56
references.pmid: 18092339
references.citation: Cancer. 1989 Aug 1;64(3):598-604
references.pmid: 2743254
references.citation: Eur J Nutr. 2008 May;47 Suppl 2:51-9
references.pmid: 18458834
references.citation: Cancer Res. 1981 Sep;41(9 Pt 2):3685-90
references.pmid: 6266659
references.citation: Cancer Metastasis Rev. 1997 Sep-Dec;16(3-4):377-92
references.pmid: 9433646
references.citation: Exp Gerontol. 2006 Oct;41(10):1007-13
references.pmid: 16930903
references.citation: Mech Ageing Dev. 1977 Nov-Dec;6(6):413-29
references.pmid: 926867
references.citation: Biochem Soc Trans. 2007 Nov;35(Pt 5):1358-63
references.pmid: 17956351
references.citation: Age (Dordr). 2005 Sep;27(3):213-23
references.pmid: 23598654
references.citation: Drug Dev Res. 2008 Nov 1;69(7):460-471
references.pmid: 19884979
references.citation: ACS Chem Biol. 2007 Apr 24;2(4):231-6
references.pmid: 17455900
references.citation: Biogerontology. 2006 Jun;7(3):127-33
references.pmid: 16732407
references.citation: Mech Ageing Dev. 2007 Jan;128(1):45-9
references.pmid: 17118426
references.citation: Int J Cancer. 2005 Apr 20;114(4):628-33
references.pmid: 15609322
references.citation: Ann N Y Acad Sci. 2007 Apr;1100:442-8
references.pmid: 17460209
references.citation: Hormones (Athens). 2008 Jan-Mar;7(1):46-61
references.pmid: 18359744
references.citation: Cell Mol Biol (Noisy-le-grand). 2002 Sep;48(6):725-31
references.pmid: 12396085
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Aug 15;871(2):314-21
references.pmid: 18495560
references.citation: Ann N Y Acad Sci. 2006 May;1067:120-8
references.pmid: 16803977
references.citation: Am J Clin Nutr. 2008 Apr;87(4):978-84
references.pmid: 18400722
references.citation: Trends Biotechnol. 2002 Apr;20(4):147-8
references.pmid: 11906745
references.citation: Nutr Cancer. 2008;60(3):373-81
references.pmid: 18444172
references.citation: Genetics. 1974 May;77(1):71-94
references.pmid: 4366476
references.citation: Science. 2006 Aug 18;313(5789):971-5
references.pmid: 16917064
references.citation: PLoS Genet. 2009 Mar;5(3):e1000414
references.pmid: 19293945
references.citation: Aging Cell. 2006 Dec;5(6):487-94
references.pmid: 17081160
references.citation: Biotechniques. 2009 Dec;47(6 Suppl):ix-xv
references.pmid: 20041852
references.citation: Annu Rev Med. 2003;54:131-52
references.pmid: 12525670
references.citation: Nutrition. 2004 Jan;20(1):26-31
references.pmid: 14698010
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2008 Feb;149(2):314-23
references.pmid: 18024103
references.citation: J Nutr. 2008 Nov;138(11):2091-7
references.pmid: 18936203
references.citation: Mech Ageing Dev. 2007 Oct;128(10):546-52
references.pmid: 17875315
references.citation: Nature. 2004 Aug 5;430(7000):686-9
references.pmid: 15254550
references.citation: Am J Clin Nutr. 2005 Sep;82(3):497-503
references.pmid: 16155259
references.citation: ACS Chem Biol. 2006 Jun 20;1(5):277-8
references.pmid: 17163755
references.citation: Curr Mol Med. 2005 Aug;5(5):537-46
references.pmid: 16101482
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 15;853(1-2):234-41
references.pmid: 17416558
references.citation: Mini Rev Med Chem. 2005 Jan;5(1):13-20
references.pmid: 15638788
references.citation: Science. 1997 Jan 10;275(5297):218-20
references.pmid: 8985016
references.citation: Am J Clin Nutr. 2003 Sep;78(3):361-9
references.pmid: 12936916
references.citation: Curr Aging Sci. 2008 Dec;1(3):145-51
references.pmid: 20021385