Kiwifruit EIL and ERF genes involved in regulating fruit ripening.
Kiwifruit (Actinidia deliciosa) is a climacteric fruit sensitive to low concentrations of ethylene. To investigate the transcriptional mechanisms underlying kiwifruit ethylene response, transcription factors encoding four EIN3-Like (EILs) and 14 Ethylene Response Factors (ERFs) were cloned from kiwifruit. Expression of these transcription factors was examined during fruit development. The expression of transcripts of most AdERFs was higher during early fruit development, with the exception of AdERF3, which increased with maturity. Several AdERFs were apparently down-regulated by ethylene, as they were affected by the ethylene inhibitor 1-methylcyclopropene and by antisense suppression of ACO (for 1-aminocyclopropane-1-carboxylic acid oxidase) in the fruit. In contrast, AdEILs were constitutively expressed during fruit development and ripening. The transcription factors AdEIL2 and AdEIL3 activated transcription of the ripening-related genes AdACO1 and AdXET5 (xyloglucan endotransglycosylase gene) and, when overexpressed in Arabidopsis (Arabidopsis thaliana), stimulated ethylene production. The potential repressor AdERF9 suppressed this promoter activity. These results support a role for kiwifruit EILs and ERFs in transcriptional regulation of ripening-related genes and in the regulation of kiwifruit fruit-ripening processes.
Yin
Xue-Ren
XR
New Zealand Institute for Plant and Food Research, Auckland, New Zealand.
Allan
Andrew C
AC
Chen
Kun-song
KS
Ferguson
Ian B
IB
D029042:Actinidia / Q000235:genetics* / Q000254:growth & development*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D005030:Ethylenes / Q000096:biosynthesis; D005638:Fruit / Q000235:genetics* / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D017434:Protein Structure, Tertiary; D017384:Sequence Deletion; D013997:Time Factors
D005030:Ethylenes; D010940:Plant Proteins; C036216:ethylene
Plant Physiol. 2009 May;150(1):365-77
19261734
Plant Physiol. 2002 Jan;128(1):30-7
11788750
Plant Cell. 2008 Jun;20(6):1693-707
18552202
FEBS Lett. 2004 Aug 27;573(1-3):110-6
15327984
Plant Physiol. 1993 Oct;103(2):669-70
8029342
Plant Cell. 2000 May;12(5):771-86
10810149
Biochemistry. 2002 Apr 2;41(13):4202-8
11914065
Plant J. 2001 Apr;26(1):47-58
11359609
Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18822-7
17030801
Plant Cell. 1998 Sep;10(9):1571-80
9724702
Annu Rev Cell Dev Biol. 2000;16:1-18
11031228
Plant J. 1999 Nov;20(4):475-83
10607299
Curr Opin Plant Biol. 2008 Oct;11(5):479-85
18692429
Plant Mol Biol. 2005 Jul;58(4):585-96
16021341
J Exp Bot. 2007;58(13):3671-8
17965144
J Exp Bot. 2008;59(15):4195-204
18953072
Plant Mol Biol. 2006 May;61(1-2):141-52
16786297
Plant Physiol. 2007 Jan;143(1):400-9
17114278
Mol Plant Pathol. 2009 Jul;10(4):471-85
19523101
J Plant Physiol. 2008 Apr 18;165(6):662-70
17570560
J Biol Chem. 2008 Mar 7;283(10):6261-71
18089556
Planta. 1998 Feb;204(2):242-51
9487728
Plant Cell Physiol. 2008 Apr;49(4):512-25
18281696
Plant Physiol. 2006 Feb;140(2):411-32
16407444
Plant Cell Rep. 2007 Nov;26(11):1999-2008
17639404
Plant Cell. 2003 Dec;15(12):3033-50
14630974
Brief Bioinform. 2004 Jun;5(2):150-63
15260895
Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3985-90
15004278
J Agric Food Chem. 2009 Apr 8;57(7):2875-81
19334761
Plant Cell. 2007 Apr;19(4):1278-94
17449808
Physiol Plant. 2008 Jun;133(2):435-48
18346078
Plant Methods. 2005 Dec 18;1:13
16359558
J Exp Bot. 2008;59(8):2097-108
18535296
Plant Cell. 2000 Mar;12(3):393-404
10715325
J Exp Bot. 2009;60(3):907-22
19213809
Genes Dev. 1998 Dec 1;12(23):3703-14
9851977
Plant Physiol. 2003 Jul;132(3):1475-88
12857828
J Exp Bot. 2003 Oct;54(391):2239-44
12909687
J Exp Bot. 2007;58(13):3743-8
18057044
J Exp Bot. 2003 Dec;54(393):2775-6
14623944
Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):4062-7
18316719
Plant Methods. 2008 Jul 07;4:17
18601751
Plant Cell. 1995 Feb;7(2):173-82
7756828
Plant Cell. 2001 Aug;13(8):1959-68
11487705
Plant Cell. 2004 Sep;16(9):2463-80
15319479
Cell. 2003 Dec 12;115(6):679-89
14675533
Plant J. 2007 Feb;49(3):414-27
17181777
Nature. 2003 Oct 2;425(6957):521-5
14523448
Curr Opin Plant Biol. 2004 Feb;7(1):40-9
14732440
BMC Genomics. 2008 Jul 27;9:351
18655731
J Exp Bot. 2006;57(14):3825-36
17032731
Plant Mol Biol. 1997 May;34(1):45-55
9177311
FEBS Lett. 2003 Aug 28;550(1-3):149-54
12935902
Nucleic Acids Res. 2000 Feb 15;28(4):960-7
10648789
Science. 2000 Dec 15;290(5499):2105-10
11118137
Cell. 1997 Jun 27;89(7):1133-44
9215635
Planta. 2009 May;229(6):1323-34
19306102