title: [Prokaryote diversity in water environment of land-ocean ecotone of Zhuhai City].
abstract: By constructing 16S rDNA clone library with PCR-RFLP, the prokaryote diversity in the seawater and groundwater of land-ocean ecotone of Zhuhai City was investigated, and the similarity and cluster analyses were implemented with the database of the sequences in Genbank. In the seawater, Proteobacteria was dominant, followed by Archaeon, Gemmatimonadetes, Candidate division OP3 and OP8, and Planctomycetes, etc.; while in the groundwater, Archaeon was dominant, followed by Proteobacteria, Sphingobacteria, Candidate division OP3, Actinobacterium, and Pseudomonas. The dominant taxa in the groundwater had high similarity to the unculturable groups of marine microorganisms. Large amount of bacteria capable of degrading organic matter and purifying water body existed in the groundwater, suggesting that after long-term evolution, the land-ocean ecotone of Zhuhai City had the characteristics of both land and ocean.
authors.affiliation: School of Geography and Planning, Sun Yat-sen University, Guangzhou 510275, China. huangxiaolanlan@163.com
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Huang
authors.forename: Jian-Yao
authors.initials: JY
authors.lastname: Chen
authors.forename: Shi-Ning
authors.initials: SN
authors.lastname: Zhou
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Xie
authors.forename: Cong-Sheng
authors.initials: CS
authors.lastname: Fu
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D002681:China; D019641:DNA, Archaeal / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000235:genetics; D004784:Environmental Monitoring; D005618:Fresh Water; D009792:Oceans and Seas; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020560:Proteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal