Polymer microlenses for quantifying cell sheet mechanics.
Mechanical interactions between individual cells and their substrate have been studied extensively over the past decade; however, understanding how these interactions change as cells interact with neighboring cells in the development of a cell sheet, or early stage tissue, is less developed. We use a recently developed experimental technique for quantifying the mechanics of confluent cell sheets. Living cells are cultured on a thin film of polystyrene [PS], which is attached to a patterned substrate of crosslinked poly(dimethyl siloxane) [PDMS] microwells. As cells attach to the substrate and begin to form a sheet, they apply sufficient contractile force to buckle the PS film over individual microwells to form a microlens array. The curvature for each microlens is measured by confocal microscopy and can be related to the strain and stress applied by the cell sheet using simple mechanical analysis for the buckling of thin films. We demonstrate that this technique can provide insight into the important materials properties and length scales that govern cell sheet responses, especially the role of stiffness of the substrate. We show that intercellular forces can lead to significantly different behaviors than the ones observed for individual cells, where focal adhesion is the relevant parameter.
Miquelard-Garnier
Guillaume
G
Polymer Science & Engineering Department, University of Massachusetts, 120 Governors Drive, Amherst, 01002, MA, USA.
Zimberlin
Jessica A
JA
Sikora
Christian B
CB
Wadsworth
Patricia
P
Crosby
Alfred
A
Science. 2005 Nov 18;310(5751):1139-43
16293750
Science. 1995 Feb 10;267(5199):883-5
7846531
Nat Cell Biol. 2001 May;3(5):466-72
11331874
Biophys J. 2009 Mar 4;96(5):2009-22
19254561
Cell Motil Cytoskeleton. 2008 Sep;65(9):762-7
18615631
Biophys J. 2000 Jul;79(1):144-52
10866943
Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1484-9
12552122
Int J Radiat Biol. 1998 Sep;74(3):397-403
9737542
J Cell Biol. 1994 Dec;127(6 Pt 2):1957-64
7806573
J Anat. 1971 Apr;108(Pt 3):509-17
5103420
Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2390-5
15695588
Cell Motil Cytoskeleton. 2005 Jan;60(1):24-34
15573414
Biophys J. 2007 Dec 15;93(12):4453-61
18045965
Nat Mater. 2004 Aug;3(8):545-50
15247909
Cell Motil Cytoskeleton. 1996;35(3):269-80
8913646
Curr Biol. 2005 Apr 26;15(8):724-31
15854904
Science. 1980 Apr 11;208(4440):177-9
6987736
J Exp Med. 1972 Apr 1;135(4):719-34
4336123
Exp Cell Res. 1969 Jan;54(1):107-10
5771088