title: The distribution and expression of claudin-5 and occludin at the rat blood-optic nerve barrier after borneol treatment.
abstract: The purpose of this study is to investigate the distribution and expression of the tight junction membrane proteins, claudin-5 and occludin, in rat blood-optic nerve barrier after borneol treatment. Seventy-two female Wistar rats were randomly divided into the borneol gastric lavage group and the equal volume solvent gastric lavage control group. The bilateral optic nerve from the retrobulbar region to the optic chiasma was collected from the rats in the two groups before gastric lavage and at 30 min, 1, 2, 4, and 8 h after gastric lavage. The distribution and expression of claudin-5 and occludin were detected using immunofluorescence staining, Western blotting, and reverse transcription polymerase chain reaction (RT-PCR). Results showed that claudin-5 translocated from the cell membrane to the cytoplasm at 30 min following initiation of borneol treatment, and this translocation peaked at 1 h. During this period of time, a small amount of occludin also translocated from the cell membrane to the cytoplasm. Four hours after initiation of treatment, claudin-5 and occludin levels in the cytoplasm began to decrease and were restored to their normal pattern 8 h after initiation of treatment. There were no significant differences in the levels of claudin-5 or occludin before or after treatment in either group. It was concluded that claudin-5 and occludin translocate within cells of the rat blood-optic nerve barrier after borneol treatment, and this translocation was reversible. Claudin-5 may play a potential role in permeability of the blood-optic nerve barrier following borneol treatment.
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital of Harbin Medical University, No.23 Youzheng Street, Harbin, 150001, China.
authors.forename: Di
authors.initials: D
authors.lastname: Jin
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Lijun
authors.initials: L
authors.lastname: Qu
authors.forename: Zhituo
authors.initials: Z
authors.lastname: Li
authors.forename: Lianhong
authors.initials: L
authors.lastname: Jin
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Xu
authors.forename: Hao
authors.initials: H
authors.lastname: Cui
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D049428:Blood-Nerve Barrier; D001892:Camphanes / Q000627:therapeutic use*; D062507:Claudin-5; D005260:Female; D005751:Gastric Lavage; D005786:Gene Expression Regulation; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000378:metabolism; D062793:Occludin; D009900:Optic Nerve / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry; D019108:Tight Junctions / Q000378:metabolism
chemical_list: D001892:Camphanes; D062507:Claudin-5; C491510:Cldn5 protein, rat; D008565:Membrane Proteins; D062793:Occludin; C568987:Ocln protein, rat; D012997:Solvents; C022871:isoborneol
references.citation: Zhongguo Yao Li Xue Bao. 1990 Jan;11(1):42-4
references.pmid: 2403013
references.citation: Trends Neurosci. 2001 Dec;24(12):719-25
references.pmid: 11718877
references.citation: Drug Metab Dispos. 1994 Jan-Feb;22(1):99-105
references.pmid: 8149897
references.citation: J Am Soc Nephrol. 1999 Jun;10(6):1337-45
references.pmid: 10361874
references.citation: Zhong Yao Cai. 2004 Dec;27(12):937-9
references.pmid: 15807248
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2004 Jul;24(7):632-4
references.pmid: 15307705
references.citation: J Cell Biol. 1993 Dec;123(6 Pt 2):1777-88
references.pmid: 8276896
references.citation: J Neuroimmunol. 2006 Apr;173(1-2):45-55
references.pmid: 16406118
references.citation: Vascul Pharmacol. 2002 Jun;38(6):323-37
references.pmid: 12529927
references.citation: Exp Cell Res. 2004 Oct 15;300(1):202-12
references.pmid: 15383327
references.citation: Exp Cell Res. 2003 Nov 1;290(2):275-88
references.pmid: 14567987
references.citation: Graefes Arch Clin Exp Ophthalmol. 2010 Jan;248(1):59-63
references.pmid: 19816703
references.citation: Eur J Cell Biol. 2000 Oct;79(10):707-17
references.pmid: 11089919
references.citation: Adv Drug Deliv Rev. 2001 Mar 1;46(1-3):247-79
references.pmid: 11259843
references.citation: Prog Neurobiol. 2002 Dec;68(5):311-23
references.pmid: 12531232
references.citation: J Cell Biol. 2002 Mar 18;156(6):1099-111
references.pmid: 11889141
references.citation: J Comp Neurol. 1966 Jan;126(1):109-21
references.pmid: 4956780
references.citation: J Cell Biol. 1999 Dec 13;147(6):1351-63
references.pmid: 10601346