Production and genetic improvement of a novel antimycotic agent, saadamycin, against dermatophytes and other clinical fungi from endophytic Streptomyces sp. Hedaya48.
As a part of our ongoing efforts towards finding novel antimycotic agents from marine microflora of the Red Sea, vanillin, 5,7-dimethoxy-4-p-methoxylphenylcoumarin and the new antimycotic compound saadamycin were isolated from endophytic Streptomyces sp. Hedaya48. The producing strain was isolated from the Egyptian sponge Aplysina fistularis and subjected to different UV irradiation doses. A mutant strain Ah22 with 10.5-fold (420 mg/l as compared to 40 mg/l produced by the parental strain) improved saadamycin production was isolated. Production of saadamycin from mutant Ah22 was enhanced to 2.26-fold (950 mg/l) and 2.38-fold (1000 mg/l) under optimized culture conditions in batch culture and bioreactors, respectively. Both saadamycin and 5,7-dimethoxy-4-p-methoxylphenylcoumarin exhibited significant antimycotic activity against dermatophytes and other clinical fungi.
El-Gendy
Mervat M A
MM
Department of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, Giza, Egypt.
El-Bondkly
Ahmed M A
AM
D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects*; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D016296:Mutagenesis; D011161:Porifera / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
D000935:Antifungal Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
Arch Microbiol. 2001 Nov;176(5):386-90
11702082
J Nat Prod. 1988 Sep-Oct;51(5):851-6
3204377
Microbes Infect. 2003 May;5(6):535-44
12758283
J Antibiot (Tokyo). 1996 Aug;49(8):775-80
8823510
J Antibiot (Tokyo). 2008 Mar;61(3):149-57
18503193
J Antibiot (Tokyo). 2008 Jun;61(6):379-86
18667786
Dermatol Clin. 2003 Jul;21(3):577-87
12956209
Nat Prod Rep. 2005 Jun;22(3):369-85
16010346
J Antibiot (Tokyo). 1997 Mar;50(3):206-11
9127191
Appl Environ Microbiol. 2003 Jan;69(1):634-40
12514051
Arch Immunol Ther Exp (Warsz). 1976;24(6):811-20
13763
FEMS Microbiol Ecol. 2007 Jan;59(1):47-63
17059482
Antimicrob Agents Chemother. 1996 Feb;40(2):279-91
8834867
Appl Biochem Biotechnol. 2008 Jul;150(1):85-96
18551256