title: Isolation and characterization of enantioselective DNA aptamers for ibuprofen.
abstract: Single stranded DNA aptamers that can bind to ibuprofen, a widely used anti-inflammation drug, were selected from random DNA library of 10(15) nucleotides by FluMag-SELEX process. Five different sequences were selected and their enantioselectivity and affinity were characterized. Three out of five aptamer candidates did not show any affinity to (S)-ibuprofen, but only to racemic form of ibuprofen, suggesting that they are (R)-ibuprofen specific aptamers. Another two aptamer candidates showed affinity to both racemic form and (S)-ibuprofen, which were considered as (S)-ibuprofen specific aptamers. The affinity of five ssDNA aptamers isolated was in a range of 1.5-5.2microM. In addition, all of these five aptamers did not show any affinity to analogues of ibuprofen in its profen's group (fenoprofen, flubiprofen, and naproxen) and the antibiotics of oxytetracycline, another control.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-Gu, Seoul 136-701, South Korea.
authors.forename: Yeon Seok
authors.initials: YS
authors.lastname: Kim
authors.forename: Chang Jun
authors.initials: CJ
authors.lastname: Hyun
authors.forename: In Ae
authors.initials: IA
authors.lastname: Kim
authors.forename: Man Bock
authors.initials: MB
authors.lastname: Gu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000302:isolation & purification*; D001483:Base Sequence / Q000187:drug effects; D001665:Binding Sites; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded / Q000378:metabolism*; D015723:Gene Library; D007052:Ibuprofen / Q000737:chemistry* / Q000494:pharmacology; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D009841:Oligonucleotides / Q000737:chemistry*; D052156:SELEX Aptamer Technique; D013329:Structure-Activity Relationship
chemical_list: D052157:Aptamers, Nucleotide; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D007052:Ibuprofen