BOLD fMRI of visual and somatosensory-motor stimulations in baboons.
Baboon, with its large brain size and extensive cortical folding compared to other non-human primates, serves as a good model for neuroscience research. This study reports the implementation of a baboon model for blood oxygenation level-dependent (BOLD) fMRI studies (1.5 x 1.5 x 4 mm resolution) on a clinical 3T-MRI scanner. BOLD fMRI responses to hypercapnic (5% CO(2)) challenge, 10 Hz flicker visual, and vibrotactile somatosensory-motor stimulations were investigated in baboons anesthetized sequentially with isoflurane and ketamine. Hypercapnia evoked robust BOLD increases. Paralysis was determined to be necessary to achieve reproducible functional activations within and between subjects under our experimental conditions. With optimized anesthetic doses (0.8-1.0% isoflurane or 6-8 mg/kg/h ketamine) and adequate paralysis (vecuronium, 0.2 mg/kg), robust activations were detected in the visual (V), primary (S1) and secondary (S2) somatosensory, primary motor (M cortices), supplementary motor area (SMA), lateral geniculate nucleus (LGN) and thalamus (Th). Data were tabulated for 11 trials under isoflurane and 10 trials under ketamine on 5 baboons. S1, S2, M, and V activations were detected in essentially all trials (90-100% of the time, except 82% for S2 under isoflurane and 70% for M under ketamine). LGN activations were detected 64-70% of the time under both anesthetics. SMA and Th activations were detected 36-45% of the time under isoflurane and 60% of the time under ketamine. BOLD percent changes among different structures were slightly higher under ketamine than isoflurane (0.75% versus 0.58% averaging all structures), but none was statistically different (P>0.05). This baboon model offers an opportunity to non-invasively image brain functions and dysfunctions in large non-human primates.
Wey
Hsiao-Ying
HY
Research Imaging Institute, University of Texas Health Science Center, San Antonio, TX 78229, USA.
Li
Jinqi
J
Szabó
C Akos
CA
Fox
Peter T
PT
Leland
M Michelle
MM
Jones
Lisa
L
Duong
Timothy Q
TQ
D000818:Animals; D005073:Evoked Potentials, Somatosensory / Q000502:physiology*; D005074:Evoked Potentials, Visual / Q000502:physiology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D010101:Oxygen Consumption / Q000502:physiology*; D048529:Papio hamadryas; D010775:Photic Stimulation; D010812:Physical Stimulation; D013003:Somatosensory Cortex / Q000502:physiology*; D014793:Visual Cortex / Q000502:physiology*
Methods. 2010 Mar;50(3):125-35
19665567
Methods. 2006 Mar;38(3):210-20
16481198
Neuron. 1998 Jun;20(6):1051-7
9655492
J Cereb Blood Flow Metab. 2000 Jan;20(1):201-6
10616809
Stroke. 1986 Nov-Dec;17(6):1254-65
3810730
J Neurosci Methods. 2001 May 30;107(1-2):71-80
11389943
Magn Reson Med. 2004 Aug;52(2):277-85
15282809
Nat Neurosci. 1999 Jun;2(6):555-62
10448221
Neuroreport. 2001 Jul 20;12(10):2231-6
11447340
Neuroimage. 2004;23 Suppl 1:S220-33
15501093
Philos Trans R Soc Lond B Biol Sci. 2002 Aug 29;357(1424):1003-37
12217171
Fed Proc. 1979 Sep;38(10):2429-33
39008
J Neurophysiol. 1969 Mar;32(2):170-83
4975532
Hum Brain Mapp. 2009 Apr;30(4):1328-37
18537113
Neuroimage. 2004 Jul;22(3):1351-70
15219607
J Neurosci Methods. 2005 Oct 30;148(2):154-60
15964078
Eur J Neurosci. 1999 Dec;11(12):4451-6
10594672
Neuron. 2001 Nov 20;32(4):565-77
11719199
Magn Reson Med. 2000 Aug;44(2):231-42
10918322
Magn Reson Med. 2001 Sep;46(3):436-42
11550233
Neuroreport. 2001 Aug 8;12(11):2335-40
11496106
Am J Primatol. 2004 Mar;62(3):139-64
15027089
J Neurosci Methods. 2002 Aug 30;118(2):141-52
12204305
Respir Med. 2003 May;97(5):476-81
12735663
J Magn Reson Imaging. 2004 Feb;19(2):168-75
14745749
J Neurosci. 2009 May 27;29(21):7031-9
19474330
Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9718-23
10931954
J Neurophysiol. 2007 Jan;97(1):264-71
17065253
Neuroimage. 2009 Jul 1;46(3):559-68
19303450
Am J Hum Genet. 1997 Sep;61(3):489-93
9326312
NMR Biomed. 2001 Nov-Dec;14(7-8):408-12
11746933
J Neurosci. 2009 Mar 11;29(10):3036-44
19279240
Anesthesiology. 1999 Sep;91(3):677-80
10485778
Stroke. 1999 Dec;30(12):2752-8
10583007
Epilepsia. 2007 Feb;48(2):245-53
17295617
Magn Reson Med. 2000 Mar;43(3):383-92
10725881
Neuroimage. 2007 Apr 15;35(3):1044-63
17321759
Immunol Rev. 2001 Oct;183:127-40
11782253
J Appl Physiol Respir Environ Exerc Physiol. 1979 Nov;47(5):954-60
511720
Brain. 1999 Oct;122 ( Pt 10):1989-97
10506099
J Neurosurg. 1987 Jul;67(1):34-43
3298565
J Neurosci. 2007 Aug 22;27(34):9181-91
17715354
Methods. 2010 Mar;50(3):136-46
19665566
Magn Reson Med. 2002 Apr;47(4):736-41
11948735
Neuroimage. 2004;23 Suppl 1:S208-19
15501092
J Cereb Blood Flow Metab. 2004 Jul;24(7):764-70
15241184
Brain Res Bull. 2002 Aug 15;58(4):429-38
12183022
Nat Neurosci. 2000 Feb;3(2):105-7
10649563
Neuroimage. 2008 Sep 1;42(3):1110-7
18598771
Brain Res Brain Res Rev. 1989 Jul-Sep;14(3):245-78
2679941
ILAR J. 2003;44(2):96-104
12652004
Neurosci Lett. 2002 May 17;324(2):125-8
11988343
Brain Res. 1999 May 22;829(1-2):167-73
10350543
Neuroimage. 2008 Jan 1;39(1):248-60
17904868
Nature. 2001 Jul 12;412(6843):150-7
11449264
Proc Natl Acad Sci U S A. 2005 May 10;102(19):6996-7001
15860578
Neuroimage. 2002 Sep;17(1):421-30
12482095
Magn Reson Med. 2004 Jul;52(1):89-99
15236371
Magn Reson Med. 1998 Apr;39(4):615-24
9543424
Hum Brain Mapp. 2005 Mar;24(3):248-57
15654698
Neuroimage. 2009 Mar;45(1 Suppl):S173-86
19059349
Folia Primatol (Basel). 1981;35(1):1-29
7014398
Vaccine. 1997 Jun;15(8):903-8
9234544
J Neurosci Methods. 2009 Oct 30;184(1):129-35
19631690
Cereb Cortex. 2001 May;11(5):463-73
11313298
Nat Neurosci. 2000 Feb;3(2):164-9
10649572
J Cereb Blood Flow Metab. 2003 Apr;23(4):472-81
12679724
Neuroimage. 2003 Dec;20(4):2040-50
14683708