title: Transcriptomes of the desiccation-tolerant resurrection plant Craterostigma plantagineum.
abstract: Studies of the resurrection plant Craterostigma plantagineum have revealed some of the mechanisms which these desiccation-tolerant plants use to survive environments with extreme dehydration and restricted seasonal water. Most resurrection plants are polyploid with large genomes, which has hindered efforts to obtain whole genome sequences and perform mutational analysis. However, the application of deep sequencing technologies to transcriptomics now permits large-scale analyses of gene expression patterns despite the lack of a reference genome. Here we use pyro-sequencing to characterize the transcriptomes of C. plantagineum leaves at four stages of dehydration and rehydration. This reveals that genes involved in several pathways, such as those required for vitamin K and thiamin biosynthesis, are tightly regulated at the level of gene expression. Our analysis also provides a comprehensive picture of the array of cellular responses controlled by gene expression that allow resurrection plants to survive desiccation.
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes vej. 5, 2200, Copenhagen, Denmark.
authors.forename: Maria C Suarez
authors.initials: MCS
authors.lastname: Rodriguez
authors.affiliation: Department of Systems Biology, Technical University of Denmark, Kemitorvet 208, 2800 Lyngby, Denmark.
authors.forename: Daniel
authors.initials: D
authors.lastname: Edsgärd
authors.affiliation: Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, Kirschallee 1, D-53115 Bonn, Germany.
authors.forename: Syed S
authors.initials: SS
authors.lastname: Hussain
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes vej. 5, 2200, Copenhagen, Denmark.
authors.forename: David
authors.initials: D
authors.lastname: Alquezar
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes vej. 5, 2200, Copenhagen, Denmark.
authors.forename: Morten
authors.initials: M
authors.lastname: Rasmussen
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes vej. 5, 2200, Copenhagen, Denmark.
authors.forename: Thomas
authors.initials: T
authors.lastname: Gilbert
authors.affiliation: Department of Systems Biology, Technical University of Denmark, Kemitorvet 208, 2800 Lyngby, Denmark.
authors.forename: Bjørn H
authors.initials: BH
authors.lastname: Nielsen
authors.affiliation: Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, Kirschallee 1, D-53115 Bonn, Germany.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Bartels
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes vej. 5, 2200, Copenhagen, Denmark.
authors.forename: John
authors.initials: J
authors.lastname: Mundy
mesh_terms: D032181:Craterostigma / Q000235:genetics / Q000378:metabolism*; D003681:Dehydration; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D018749:RNA, Plant / Q000235:genetics; D017422:Sequence Analysis, DNA; D014867:Water / Q000378:metabolism
chemical_list: D018749:RNA, Plant; D014867:Water
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