Gbeta5 recruits R7 RGS proteins to GIRK channels to regulate the timing of neuronal inhibitory signaling.
The type 5 G protein beta subunit (Gbeta5) can form complexes with members of the regulator of G protein signaling 7 (RGS7) family, but its relevance to neuronal G protein signaling is unclear. We found that mouse RGS7-Gbeta5 complexes bound to G protein-gated potassium channels and facilitated their functional coupling to GABA(B) receptors in neurons. Our findings identify a compartmentalization mechanism that is critical for ensuring high temporal resolution of neuronal G protein signaling.
Xie
Keqiang
K
Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota, USA.
Allen
Kevin L
KL
Kourrich
Saïd
S
Colón-Saez
José
J
Thomas
Mark J
MJ
Wickman
Kevin
K
Martemyanov
Kirill A
KA
D000818:Animals; D001418:Baclofen / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels / Q000235:genetics / Q000378:metabolism*; D018755:GABA Agonists / Q000494:pharmacology; D044387:GTP-Binding Protein beta Subunits / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology; D006801:Humans; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D020710:RGS Proteins / Q000378:metabolism*; D018080:Receptors, GABA-B / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; D018755:GABA Agonists; D044387:GTP-Binding Protein beta Subunits; C094668:Gnb5 protein, mouse; D020710:RGS Proteins; D018080:Receptors, GABA-B; C492038:Rgs7 protein, mouse; D001418:Baclofen
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