Physiological and pharmacological characterizations of the larval Anopheles albimanus rectum support a change in protein distribution and/or function in varying salinities.
Ion regulation is a biological process crucial to the survival of mosquito larvae and a major organ responsible for this regulation is the rectum. The recta of anopheline larvae are distinct from other subfamilies of mosquitoes in several ways, yet have not yet been characterized extensively. Here we characterize the two major cell types of the anopheline rectum, DAR and non-DAR cells, using histological, physiological, and pharmacological analyses. Proton flux was measured at the basal membrane of 2%- and 50%-artificial sea water-reared An. albimanus larvae using self-referencing ion-selective microelectrodes, and the two cell types were found to differ in basal membrane proton flux. Additionally, differences in the response of that flux to pharmacological inhibitors in larvae reared in 2% versus 50% ASW indicate changes in protein function between the two rearing conditions. Finally, histological analyses suggest that the non-DAR cells are structurally suited for mediating ion transport. These data support a model of rectal ion regulation in which the non-DAR cells have a resorptive function in freshwater-reared larvae and a secretive function in saline water-reared larvae. In this way, anopheline larvae may adapt to varying salinities.
Smith
Kristin E
KE
The Whitney Laboratory for Marine Bioscience, University of Florida, 9505 OceanShore Boulevard, St. Augustine, FL 32080, USA.
Raymond
Steven L
SL
Valenti
Micheala L
ML
Smith
Peter J S
PJ
Linser
Paul J
PJ
D000223:Adaptation, Psychological / Q000187:drug effects / Q000502:physiology; D000818:Animals; D000852:Anopheles / Q000033:anatomy & histology / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005618:Fresh Water; D019476:Insect Proteins / Q000032:analysis / Q000378:metabolism*; D017136:Ion Transport / Q000187:drug effects; D007814:Larva / Q000033:anatomy & histology / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism; D008839:Microelectrodes; D017494:Proton Pumps / Q000187:drug effects / Q000378:metabolism; D011522:Protons; D012007:Rectum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D054712:Salinity; D012623:Seawater; D012965:Sodium Chloride / Q000494:pharmacology
D019476:Insect Proteins; D008565:Membrane Proteins; D017494:Proton Pumps; D011522:Protons; D012965:Sodium Chloride
J Exp Biol. 2008 Oct;211(Pt 19):3067-76
18805805
J Biol Chem. 2001 Dec 21;276(51):47886-94
11606574
Insect Mol Biol. 2008 Feb;17(1):61-72
18237285
Biochim Biophys Acta. 2001 May 1;1505(1):144-57
11248196
J Biol Chem. 2002 Oct 25;277(43):40544-8
12186879
J Exp Biol. 1992 Nov 1;172(Pt 1):417-429
9874752
J Exp Biol. 1977 Feb;66(1):111-26
858991
J Exp Biol. 2006 Sep;209(Pt 18):3529-44
16943493
J Biol Chem. 1986 Jul 5;261(19):8778-83
3013862
JOP. 2001 Jul;2(4 Suppl):176-81
11875256
J Enzyme Inhib Med Chem. 2004 Jun;19(3):231-6
15499994
Annu Rev Entomol. 1987;32:439-62
2880552
Biophys J. 2005 Sep;89(3):1721-30
15994902
J Virol. 1977 Jun;22(3):778-93
559779
J Exp Biol. 2008 Mar;211(Pt 6):957-68
18310121
Methods Enzymol. 1990;192:608-16
2150092
J Exp Biol. 2007 Nov;210(Pt 21):3848-61
17951426
Am J Physiol Renal Physiol. 2007 May;292(5):F1501-12
17287198
J Exp Biol. 2007 Nov;210(Pt 22):3919-30
17981859
Z Zellforsch Mikrosk Anat. 1973 Mar 21;138(1):1-22
4348887
Proc Natl Acad Sci U S A. 1964 May;51:757-65
14172989
Nature. 2002 Feb 7;415(6872):680-5
11832956
World Health Forum. 1997;18(1):1-8
9233055
J Exp Biol. 1999 Feb;202(Pt 3):247-52
9882637
Pflugers Arch. 2004 Feb;447(5):495-509
14722772
Pflugers Arch. 2004 Feb;447(5):549-65
12845533
Microsc Res Tech. 1999 Sep 15;46(6):398-417
10504217
J Biol Chem. 1991 Aug 15;266(23):15340-7
1831202
Exp Eye Res. 1988 Oct;47(4):515-23
3181332