Facile preparation of deuterium-labeled N-acylhomoserine lactones as internal standards for isotope dilution mass spectrometry.
N-Acylhomoserine lactones (AHLs) are widely conserved signal molecules that mediate quorum sensing in Gram-negative bacteria. In this study, deuterium-labeled AHLs were prepared for use as internal standards for isotope dilution mass spectrometry. Their utility in the sensitive and precise quantification of AHLs in culture supernatants of bacteria by GC/MS was demonstrated.
Kai
Kenji
K
Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. kai@biochem.osakafu-u.ac.jp
Tani
Ayaka
A
Hayashi
Hideo
H
D054742:Acyl-Butyrolactones / Q000138:chemical synthesis / Q000592:standards*; D001419:Bacteria / Q000737:chemistry; D017077:Culture Media, Conditioned / Q000032:analysis; D003903:Deuterium / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007201:Indicator Dilution Techniques; D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000379:methods* / Q000592:standards; D053038:Quorum Sensing; D012015:Reference Standards
D054742:Acyl-Butyrolactones; D017077:Culture Media, Conditioned; D003903:Deuterium