Expression of microRNA-451 in normal and thalassemic erythropoiesis.
MicroRNAs (miRNAs) are negative regulators of gene expression that play an important role in hematopoiesis. Thalassemia, a defective globin synthesis leading to precipitate of excess unbound globins in red blood cell precursors, results in defective erythroid precursors and ineffective erythropoiesis. Expression pattern of miR-451, an erythroid-specific miRNA, was analyzed during differentiation of erythroid progenitors derived from normal and thalassemic peripheral blood CD34-positive cells, after 14 days of culture. A biphasic expression with transient up-regulation of miRNA-451 on day 3 of cultures was observed during thalassemic erythroid differentiation. In contrast, the expression pattern of the miR-451 in erythroid cells obtained from the other extravascular hemolytic anemia, i.e., hereditary spherocytosis patients showed no transient up-regulation of miR-451 on day 3 of cultures. Our results suggest that early erythroid progenitors in beta-thalassemia have a dysregulated miRNA-451 expression program, and analysis of microRNA is a relevant approach to determine abnormalities of erythropoiesis.
Svasti
Saovaros
S
Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakornpathom, Thailand.
Masaki
Shizuka
S
Penglong
Tipparat
T
Abe
Yasunobu
Y
Winichagoon
Pranee
P
Fucharoen
Suthat
S
Umemura
Tsukuru
T
D000328:Adult; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004920:Erythropoiesis / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D017086:beta-Thalassemia / Q000235:genetics* / Q000378:metabolism
C531201:MIRN451 microRNA, human; D035683:MicroRNAs; D005914:Globins