1Identification of genes differentially expressed in the embryonic pig cerebral cortex before and after appearance of gyration.
BACKGROUND
Mammalian evolution is characterized by a progressive expansion of the surface area of the cerebral cortex, an increase that is accompanied by gyration of the cortical surface. The mechanisms controlling this gyration process are not well characterized but mutational analyses indicate that genes involved in neuronal migration play an important function. Due to the lack of gyration of the rodent brain it is important to establish alternative models to examine brain development during the gyration process. The pig brain is gyrated and accordingly is a candidate alternative model.


RESULTS
In this study we have identified genes differentially expressed in the pig cerebral cortex before and after appearance of gyration. Pig cortical tissue from two time points in development representing a non-folded, lissencephalic, brain (embryonic day 60) and primary-folded, gyrencephalic, brain (embryonic day 80) were examined by whole genome expression microarray studies. 91 differentially expressed transcripts (fold change >3) were identified. 84 transcripts were annotated and encoding proteins involved in for example neuronal migration, calcium binding, and cytoskeletal structuring. Quantitative real-time PCR was used to confirm the regulation of a subset of the identified genes.


CONCLUSIONS
This study provides identification of genes which are differentially expressed in the pig cerebral cortex before and after appearance of brain gyration. The identified genes include novel candidate genes which could have functional importance for brain development.
Nielsen
Karsten B
KB
Department of Human Genetics, University of Aarhus, The Bartholin Building, DK-8000 Aarhus C, Denmark. bork@ki.au.dk.
Kruhøffer
Mogens
M
Holm
Ida E
IE
Jørgensen
Arne L
AL
Nielsen
Anders L
AL
Genome Biol. 2002 Jun 18;3(7):RESEARCH0034
12184808
Trends Neurosci. 1995 Sep;18(9):379-83
7482802
Trends Neurosci. 1995 Sep;18(9):383-8
7482803
Nature. 1993 Aug 19;364(6439):717-21
8355785
Nat Rev Neurosci. 2006 Nov;7(11):883-90
17033683
Hum Genet. 2002 Aug;111(2):214-8
12189496
Hum Mutat. 2007 Nov;28(11):1055-64
17584854
Mol Biol Cell. 2008 Mar;19(3):1152-61
18199681
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3196-201
14963232
Nat Genet. 2000 Sep;26(1):93-6
10973257
Science. 1988 Jul 8;241(4862):170-6
3291116
Hum Genet. 2002 Mar;110(3):279-83
11935341
Cereb Cortex. 2006 Jul;16 Suppl 1:i152-61
16766701
Cell. 2007 Jan 12;128(1):29-43
17218253
J Comp Neurol. 1972 May;145(1):61-83
4624784
Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1242-6
9448316
PLoS Comput Biol. 2006 Mar;2(3):e22
16557292
Anim Genet. 2006 Aug;37(4):423-4
16879364
J Cell Sci. 2008 Sep 1;121(Pt 17):2783-93
18716282
Cell. 2007 Jan 12;128(1):45-57
17218254
Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12593-8
18689685
Nature. 1997 Jan 23;385(6614):313-8
9002514
Cereb Cortex. 2005 Dec;15(12):1900-13
15758198
Cereb Cortex. 2002 Jan;12(1):37-53
11734531
Dev Neurosci. 2008;30(1-3):24-32
18075251
J Anat. 2007 Aug;211(2):164-76
17634059
Neuron. 2005 Aug 4;47(3):353-64
16055060
Trends Neurosci. 2004 Jul;27(7):392-9
15219738