Enhancing the virulence of Paecilomyces lilacinus against Meloidogyne incognita eggs by overexpression of a serine protease.
To enhance the virulence of Paecilomyces lilacinus against Meloidogyne incognita eggs, a serine protease was overexpressed in P. lilacinus 9410 (PL9410). The cDNA of a mature serine protease gene was cloned from PL9410 and integrated into the genomes of PL9410 transformants through Agrobacterium tumefaciens-mediated transformation. Our results confirmed that the serine protease gene was overexpressed at the transcriptional level, and that the serine protease activities were enhanced in the transformants when compared to the parent strain. The bioassay results indicated that the relative parasitizing rates of the transformants against M. incognita eggs increased by about 20% in both conidial suspension and mycelium treatment groups.
Wang
Jieping
J
School of Life Science, Xiamen University, Xiamen, 361005 Fujian, China.
Wang
Jiaxu
J
Liu
Fan
F
Pan
Cangsang
C
D000818:Animals; D010063:Ovum / Q000382:microbiology*; D010143:Paecilomyces / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity*; D010572:Pest Control, Biological; D018517:Plant Roots / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D057057:Serine Proteases / Q000378:metabolism*; D014431:Tylenchoidea / Q000382:microbiology*; D015854:Up-Regulation
D057057:Serine Proteases