title: N-cadherin-dependent neuron-neuron interaction is required for the maintenance of activity-induced dendrite growth.
abstract: Formation of neural circuits depends on stable contacts between neuronal processes, mediated by interaction of cell adhesion molecules, including N-cadherin. In the present study, we found that activity-dependent dendrite arborization specifically requires N-cadherin-mediated extracellular neuron-neuron interaction, because the enhancement did not occur for neurons cultured in isolation or plated on an astrocyte monolayer and was abolished by a recombinant soluble N-cadherin ectodomain. Furthermore, depolarization elevated the level of membrane-associated cadherin/catenin complexes and surface N-cadherin. Importantly, surface N-cadherin elevation is specifically required for the maintenance of nascent dendrite arbors. Through loss- and gain-of-function approaches, we showed that N-cadherin-mediated dendrite growth requires association of the cadherin/catenin complex with the actin cytoskeleton. In summary, these results identify a previously unexplored and specific function for activity-induced, N-cadherin-mediated neuron-neuron contacts in the maintenance of dendrite arbors.
authors.affiliation: Institute of Neuroscience and State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Shanghai 200031, China. yuxiang@ion.ac.cn
authors.forename: Zhu-Jun
authors.initials: ZJ
authors.lastname: Tan
authors.forename: Yun
authors.initials: Y
authors.lastname: Peng
authors.forename: He-Ling
authors.initials: HL
authors.lastname: Song
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zheng
authors.forename: Xiang
authors.initials: X
authors.lastname: Yu
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D051177:Catenins / Q000378:metabolism; D002448:Cell Adhesion; D002450:Cell Communication; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000199:Actins; D015820:Cadherins; D051177:Catenins
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