Crystallization and preliminary X-ray diffraction analysis of West Nile virus.
West Nile virus, a human pathogen, is closely related to other medically important flaviviruses of global impact such as dengue virus. The infectious virus was purified from cell culture using polyethylene glycol (PEG) precipitation and density-gradient centrifugation. Thin amorphously shaped crystals of the lipid-enveloped virus were grown in quartz capillaries equilibrated by vapor diffusion. Crystal diffraction extended at best to a resolution of about 25 A using synchrotron radiation. A preliminary analysis of the diffraction images indicated that the crystals had unit-cell parameters a approximately b approximately 480 A, gamma = 120 degrees , suggesting a tight hexagonal packing of one virus particle per unit cell.
Kaufmann
Bärbel
B
Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907-2054, USA.
Plevka
Pavel
P
Kuhn
Richard J
RJ
Rossmann
Michael G
MG
D020285:Cryoelectron Microscopy; D003460:Crystallization; D018360:Crystallography, X-Ray; D014902:West Nile virus / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure
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