Validation of novel promoter sequences derived from two endogenous ubiquitin genes in transgenic Aedes aegypti.
To date, only a limited number of promoter sequences have been described to drive transgene expression in the disease vector Aedes aegypti. We sought to increase this repertoire by characterizing the ability of upstream sequences derived from the Ae. aegypti Ub(L40) and polyubiquitin genes to drive the expression of marker proteins. Both genomic fragments were able to drive robust expression of luciferase in cultured mosquito cells. Following Mos1-transformation, the Ub(L40) promoter drove strong expression of a fluorescent marker in early larvae and in ovaries, while the polyubiquitin promoter drove robust EGFP expression in all stages of development, including constitutive expression throughout the midgut. These promoter fragments provide two new expression profiles for future Ae. aegypti genetic experiments.
Anderson
M A E
MA
Fralin Life Science Institute and Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
Gross
T L
TL
Myles
K M
KM
Adelman
Z N
ZN
D000330:Aedes / Q000166:cytology / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D005786:Gene Expression Regulation; D017344:Genes, Insect / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D025821:Polyubiquitin / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015203:Reproducibility of Results; D014170:Transformation, Genetic; D025801:Ubiquitin / Q000235:genetics*
D025801:Ubiquitin; C485184:enhanced green fluorescent protein; D025821:Polyubiquitin; D049452:Green Fluorescent Proteins
Proc Natl Acad Sci U S A. 2007 Jun 12;104(24):9970-5
17548819
J Virol. 2002 Dec;76(24):12925-33
12438618
Gene. 1999 Jan 21;226(2):317-25
9931506
Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3743-7
9520437
Dev Biol. 1995 Aug;170(2):726-9
7649398
Med Vet Entomol. 2009 Jun;23 Suppl 1:98-105
19335836
Mol Cell Biol. 1988 Nov;8(11):4727-35
2463465
Insect Mol Biol. 2009 Oct;18(5):623-33
19754740
Insect Biochem Mol Biol. 2006 Apr;36(4):273-81
16551541
Nucleic Acids Res. 2003 Nov 15;31(22):e147
14602940
Biochem J. 1992 Aug 15;286 ( Pt 1):281-8
1381584
Dev Genes Evol. 2000 Dec;210(12):630-7
11151300
Insect Mol Biol. 1996 May;5(2):109-17
8673261
Dev Genes Evol. 2000 Dec;210(12):623-9
11151299
Insect Biochem Mol Biol. 2008 Jul;38(7):705-13
18549956
Insect Mol Biol. 2007 Feb;16(1):61-71
17257209
Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10895-8
11005862
Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):9144-9
10908672
Lancet. 2007 Nov 10;370(9599):1644-52
17993365
Insect Biochem Mol Biol. 2001 Nov 1;31(12):1137-43
11583926
Insect Biochem Mol Biol. 2009 Apr;39(4):303-14
19552893
Insect Mol Biol. 2002 Aug;11(4):291-7
12144693
Nature. 1989 Mar 30;338(6214):394-401
2538753
Insect Mol Biol. 2006 Aug;15(4):393-401
16907826
Insect Mol Biol. 1999 Nov;8(4):449-57
10634970
Proc Natl Acad Sci U S A. 2005 Sep 20;102(38):13568-73
16157887
Insect Mol Biol. 2000 Feb;9(1):1-10
10672065
Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4198-203
16537508
Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3748-51
9520438
Insect Mol Biol. 2002 Oct;11(5):399-407
12230539
Insect Biochem Mol Biol. 2001 Feb;31(2):199-205
11164342