title: Occasional involvement of the ovary in Ewing sarcoma.
abstract: BACKGROUND
  Ewing sarcoma (EWS) is a highly metastatic malignancy in young patients. Ovarian cryopreservation is often an option for fertility preservation in cancer patients of reproductive age, specifically in minors. Thus, the possibility of ovarian involvement in EWS needs to be elucidated.
  
  
  METHODS
  Eight patients aged 13-20 years with EWS participated in the study. Ovarian samples were fixed and prepared for light microscopy, and frozen in liquid nitrogen for RNA extraction followed by RT-PCR. Histological studies, including immunostaining for the adhesion receptor CD99, were used to detect histopathological features. Sensitive molecular methods were used to detect translocations causing the formation of tumor-specific EWS-Friend leukemia virus integration site 1 fusion gene (EWS-FLI1).
  
  
  RESULTS
  In seven patients, there was no evidence of EWS in the ovaries from pathological/molecular studies. However, in one patient, the RT-PCR showed the EWS translocation, although there was no pathological evidence.
  
  
  CONCLUSIONS
  Ovarian involvement is possible in EWS. Therefore, in patients with EWS ovarian tissue should be examined for traces of malignancy at both the pathological and molecular levels prior to the grafting of cryopreserved tissue in order to minimize the risk of reseeding the cancer.
authors.affiliation: Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petah Tikva, Israel. ronita@clalit.org.il
authors.forename: Ronit
authors.initials: R
authors.lastname: Abir
authors.forename: Meora
authors.initials: M
authors.lastname: Feinmesser
authors.forename: Isaac
authors.initials: I
authors.lastname: Yaniv
authors.forename: Benjamin
authors.initials: B
authors.lastname: Fisch
authors.forename: Ian J
authors.initials: IJ
authors.lastname: Cohen
authors.forename: Avi
authors.initials: A
authors.lastname: Ben-Haroush
authors.forename: Dror
authors.initials: D
authors.lastname: Meirow
authors.forename: Carmela
authors.initials: C
authors.lastname: Felz
authors.forename: Smadar
authors.initials: S
authors.lastname: Avigad
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D015514:Oncogene Proteins, Fusion / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000453:epidemiology / Q000378:metabolism / Q000556:secondary*; D010053:Ovary / Q000378:metabolism / Q000637:transplantation; D051789:Proto-Oncogene Protein c-fli-1 / Q000378:metabolism; D034802:RNA-Binding Protein EWS; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018570:Risk Assessment; D012512:Sarcoma, Ewing / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019737:Transplants
chemical_list: C106031:EWS-FLI fusion protein; D015514:Oncogene Proteins, Fusion; D051789:Proto-Oncogene Protein c-fli-1; D034802:RNA-Binding Protein EWS