Association of -2548 G/A polymorphism in the leptin gene with preeclampsia/pregnancy-induced hypertension.
OBJECTIVE
To investigate −2548 G/A polymorphism of LEP gene, plasma leptin and soluble leptin receptor in preeclampsia/pregnancy induced hypertension.


METHODS
Sixty-two patients and 63 controls (normal pregnancies) studied in the third trimester. Leptin and soluble leptin receptor measured by enzyme immunosorbent assay and −2548 G/A polymorphism by polymerase chain reaction followed by restriction enzyme digestion.


RESULTS
Frequency of the AA genotype was significantly (p < 0.001) higher in patients. The A allele conferred a relative risk of 1.67 for the disease (p < 0.0001). Preeclampsia/pregnancy induced hypertension significantly (p < 0.001 to 0.0001) affected leptin and soluble leptin receptor concentrations, free leptin index and leptin normalized to body mass index. Genotype significantly (p < 0.05) influenced only the soluble leptin receptor concentrations.


CONCLUSIONS
Preeclampsia/pregnancy induced hypertension appears to be associated with higher circulating leptin and lower SLR levels, and with the AA genotype of −2548 G/A polymorphism of the leptin gene.
Sugathadasa
B H K R
BH
Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Sri Lanka.
Tennekoon
K H
KH
Karunanayake
E H
EH
Kumarasiri
J M
JM
Wijesundere
A P D S
AP
D000328:Adult; D000483:Alleles; D000704:Analysis of Variance; D005260:Female; D005787:Gene Frequency; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000097:blood / Q000235:genetics*; D020738:Leptin / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D054411:Receptors, Leptin / Q000097:blood / Q000235:genetics; D018709:Statistics, Nonparametric
D020738:Leptin; D054411:Receptors, Leptin