title: Assessing the potential of an induced-mutation strategy for avermectin overproducers.
abstract: Mutant libraries of avermectin-producing Streptomyces avermitilis strains were constructed by different mutagenesis strategies. A metric was applied to assess the mutation spectrum by calculating the distribution of average phenotypic distance of each population. The results showed for the first time that a microgravity environment could introduce larger phenotype distribution and diversity than UV and N-methyl-N-nitro-N-nitrosoguanidine (NTG) could.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing 100190, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.forename: Xianlong
authors.initials: X
authors.lastname: Zhou
authors.forename: Jintao
authors.initials: J
authors.lastname: Liu
authors.forename: Difei
authors.initials: D
authors.lastname: Chen
authors.forename: Wenquan
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhongxuan
authors.initials: Z
authors.lastname: Gou
authors.forename: Peng
authors.initials: P
authors.lastname: Shang
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000378:metabolism; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016296:Mutagenesis; D009154:Mutation; D010641:Phenotype; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014893:Weightlessness
chemical_list: D003470:Culture Media; D008769:Methylnitronitrosoguanidine; D007559:Ivermectin; C019264:avermectin
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