Radiation sterilization of fluoroquinolones in solid state: investigation of effect of gamma radiation and electron beam.
The effect of gamma radiation from (60)Co source and 2MeV electron beam was studied on two fluoroquinolone antibiotics viz norfloxacin and gatifloxacin in the solid state. The changes in reflectance spectrum, yellowness index, vibrational characteristics, thermal behavior, UV spectrum, chemical potency (HPLC) and microbiological potency were investigated. ESR measurement gave the number of free radical species formed and their population. The nature of final radiolytic impurities was assessed by studying the HPLC impurity profile. Both norfloxacin and gatifloxacin were observed to be radiation resistant, and did not show significant changes in their physico-chemical properties. They could be radiation sterilized at a dose of 25kGy.
Singh
Babita K
BK
Department of Chemistry, RTM Nagpur University Campus, Amravati Road, Nagpur, India. singhbab2001@rediffmail.com
Parwate
Dilip V
DV
Dassarma
Indrani B
IB
Shukla
Sudhir K
SK
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000528:radiation effects*; D018407:Bacterial Physiological Phenomena / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004340:Drug Contamination / Q000517:prevention & control*; D004583:Electrons; D024841:Fluoroquinolones / Q000737:chemistry* / Q000528:radiation effects*; D005720:Gamma Rays; D011208:Powders; D011829:Radiation Dosage; D013242:Sterilization / Q000379:methods*
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D011208:Powders