Recovery of heat shock-triggered released apoplastic Ca2+ accompanied by pectin methylesterase activity is required for thermotolerance in soybean seedlings.
Synthesis of heat shock proteins (HSPs) in response to heat shock (HS) is essential for thermotolerance. The effect of a Ca(2+) chelator, EGTA, was investigated before a lethal HS treatment in soybean (Glycine max) seedlings with acquired thermotolerance induced by preheating. Such seedlings became non-thermotolerant with EGTA treatment. The addition of Ca(2+), Sr(2+) or Ba(2+) to the EGTA-treated samples rescued the seedlings from death by preventing the increased cellular leakage of electrolytes, amino acids, and sugars caused by EGTA. It was confirmed that EGTA did not affect HSP accumulation and physiological functions but interfered with the recovery of HS-released Ca(2+) concentration which was required for thermotolerance. Pectin methylesterase (PME, EC 3.1.1.11), a cell wall remodelling enzyme, was activated in response to HS, and its elevated activity caused an increased level of demethylesterified pectin which was related to the recovery of the HS-released Ca(2+) concentration. Thus, the recovery of HS-released Ca(2+) in Ca(2+)-pectate reconstitution through PME activity is required for cell wall remodelling during HS in soybean which, in turn, retains plasma membrane integrity and co-ordinates with HSPs to confer thermotolerance.
Wu
Hui-Chen
HC
Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei, Taiwan.
Hsu
Shih-Feng
SF
Luo
Dan-Li
DL
Chen
Shiang-Jiuun
SJ
Huang
Wen-Dar
WD
Lur
Huu-Sheng
HS
Jinn
Tsung-Luo
TL
D000222:Adaptation, Physiological / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D004951:Esterification / Q000187:drug effects; D018869:Heat-Shock Response / Q000187:drug effects; D008954:Models, Biological; D015388:Organelles / Q000187:drug effects / Q000378:metabolism; D010368:Pectins / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism; D055550:Protein Stability / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology*; D012995:Solubility / Q000187:drug effects; D013025:Glycine max / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013054:Spectrophotometry, Atomic; D013194:Staining and Labeling; D013696:Temperature
D004533:Egtazic Acid; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D011096:Polygalacturonase; D002118:Calcium; C003181:polygalacturonic acid
Plant J. 2009 Mar;57(6):1015-26
19036034
Plant Physiol. 2000 Sep;124(1):231-42
10982438
Plant Cell. 2009 Sep;21(9):2829-43
19773386
Food Chem. 2008 Jul 15;109(2):368-78
26003360
Plant Physiol. 2002 May;129(1):201-10
12011351
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13504-9
12368478
Plant Physiol. 1984 Jan;74(1):152-60
16663370
Mol Biol Evol. 1999 Jan;16(1):127-39
10331257
Plant Physiol. 1996 Oct;112(2):747-57
8883386
Curr Opin Plant Biol. 1998 Oct;1(5):428-33
10066614
Plant Cell. 2005 Feb;17(2):584-96
15659637
Plant Physiol. 2004 Sep;136(1):2438-42
15375199
Nature. 1970 Aug 15;227(5259):680-5
5432063
Plant Physiol. 2006 Nov;142(3):1004-13
16980565
Trends Plant Sci. 2001 Sep;6(9):414-9
11544130
Planta. 2005 Oct;222(2):355-71
15887026
Ann Bot. 2008 Mar;101(4):521-30
18222909
Plant Mol Biol. 2001 Sep;47(1-2):9-27
11554482
Plant Physiol. 1992 Jul;99(3):1099-104
16668976
Plant Physiol. 1992 Aug;99(4):1279-84
16669033
Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet. 2002;67(4):23-6
12510581
Plant Cell Physiol. 2004 Dec;45(12):1759-67
15557296
Plant Cell. 1999 Apr;11(4):691-706
10213787
Plant Physiol. 1983 Nov;73(3):698-702
16663285
Plant Physiol. 1997 Jun;114(2):429-438
12223717
Plant Physiol. 2009 Apr;149(4):1773-84
19211698
Plant Physiol. 1991 Aug;96(4):1038-47
16668295
Plant Physiol. 2002 Feb;128(2):682-95
11842171
J Agric Food Chem. 2001 Nov;49(11):5566-75
11714361
Trends Plant Sci. 2007 Jun;12(6):267-77
17499007
Plant Physiol. 1989 Feb;89(2):617-21
16666591
Int Rev Cytol. 1994;148:171-227
8119782
J Biol Chem. 1954 Dec;211(2):907-13
13221596
Annu Rev Genet. 1993;27:437-96
8122909