Comparison of single-copy and multicopy real-time PCR targets for detection of Mycobacterium tuberculosis in paraffin-embedded tissue.
Real-time PCR can rapidly identify Mycobacterium tuberculosis in paraffin-embedded tissue in the absence of microbiological culture. In a comparison of single-copy and multicopy PCR targets in 70 tissue samples, the sensitivities were 26% and 54%, respectively, with 100% specificity. Sensitivity was 75% for newer samples and was not decreased for acid-fast bacillus (AFB) stain-negative specimens.
Luo
Robert F
RF
Stanford University School of Medicine, Department of Pathology, Stanford, CA 94305-5324, USA. rluo2@stanford.edu
Scahill
Michael D
MD
Banaei
Niaz
N
D001842:Bone and Bones / Q000382:microbiology; D006651:Histocytochemistry; D006801:Humans; D008168:Lung / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D012867:Skin / Q000382:microbiology
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