Pilot-scale production of fatty acid ethyl esters by an engineered Escherichia coli strain harboring the p(Microdiesel) plasmid.
Fatty acid ethyl esters (FAEEs) were produced in this study by the use of an engineered Escherichia coli p(Microdiesel) strain. Four fed-batch pilot scale cultivations were carried out by first using glycerol as sole carbon source for biomass production before glucose and oleic acid were added as carbon sources. Cultivations yielded a cell density of up to 61 +/- 3.1 g of cell dry mass (CDM) per liter and a maximal FAEE content of 25.4% +/- 1.1% (wt/wt) of CDM.
Elbahloul
Yasser
Y
Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, D-48149 Munster, Germany.
Steinbüchel
Alexander
A
D001674:Bioelectric Energy Sources; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004951:Esterification; D004952:Esters / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism; D010865:Pilot Projects; D010957:Plasmids / Q000235:genetics*
D003470:Culture Media; D004952:Esters; D005227:Fatty Acids; D019301:Oleic Acid; D005947:Glucose; D005990:Glycerol
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