Erythropoietin and soluble CD44 levels in patients with primary open-angle glaucoma.
OBJECTIVE
To investigate the levels of erythropoietin (EPO) and soluble CD44 (sCD44) in the aqueous and plasma of human eyes with primary open-angle glaucoma (POAG), and to correlate their concentration with severity of glaucoma.


METHODS
A total of 30 patients with POAG and 25 patients with senile cataract (control group) of matched age and gender were included in the study prospectively. Aqueous samples were obtained by paracentesis from glaucoma and cataract patients who were undergoing elective surgery. Aqueous and corresponding plasma samples were analysed for EPO and sCD44 concentrations by enzyme-linked immunosorbent assay.


RESULTS
The EPO and sCD44 levels were significantly higher in aqueous of POAG patients with respect to the comparative group of cataract patients (P < 0.001). No significant difference in the levels of EPO and sCD44 in plasma of POAG and cataract patients. A high positive correlation was found between EPO and sCD44 in aqueous of POAG patients (P < 0.001). Significant correlation was found between EPO or sCD44 levels and severity of visual field loss in mild and moderate stages (P < 0.001).


CONCLUSIONS
Increased levels of aqueous EPO and sCD44 may be associated with POAG. In addition, EPO and sCD44 may be useful proteins levels in aqueous of POAG patients as a result of glaucoma damage and not a cause. EPO and sCD44 concentrations in aqueous are a possible biomarkers for visual field loss in patients with POAG.
Mokbel
Tharwart H
TH
Ophthalmology Center, Faculty of Medicene, Mansoura University, Mansoura, Egypt.
Ghanem
Asaad A
AA
Kishk
Hanem
H
Arafa
Lamiaa F
LF
El-Baiomy
Azza A
AA
D000368:Aged; D001082:Aqueous Humor / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004921:Erythropoietin / Q000097:blood*; D005260:Female; D005902:Glaucoma, Open-Angle / Q000097:blood* / Q000503:physiopathology; D006801:Humans; D018960:Hyaluronan Receptors / Q000097:blood*; D007429:Intraocular Pressure; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies
C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D004921:Erythropoietin