title: Porphyromonas gingivalis induces RANKL in T-cells.
abstract: Porphyromonas gingivalis is an oral pathogen highly implicated in chronic periodontitis, a disease characterized by inflammatory destruction of the tooth-supporting alveolar bone and eventually, tooth loss. T-cell innate immune responses are actively involved in this pathological process. Receptor activator of NF-κB Ligand (RANKL) is a cytokine that stimulates bone resorption, while its soluble decoy receptor osteoprotegerin (OPG) blocks its action. This study aimed to investigate in Jurkat T-cells the effects of P. gingivalis on the RANKL-OPG system and the major inflammatory mediator of bone resorption prostaglandin E(2) (PGE(2)). P. gingivalis caused concentration-dependent up-regulation of RANKL gene expression and protein production, assessed by quantitative PCR and ELISA, respectively. PGE(2) production was also enhanced. However, OPG was not detected. In conclusion, P. gingivalis induces RANKL and PGE(2) in T-cells, potentially favoring bone resorption. These T-cell responses to P. gingivalis may contribute to the pathogenesis of inflammatory alveolar bone destruction occurring in chronic periodontitis.
authors.affiliation: Institute of Oral Biology, Center of Dental and Oral Medicine and Cranio-Maxillofacial Surgery, University of Zürich, Plattenstrasse 11, Zürich, Switzerland. george.belibasakis@zzmk.uzh.ch
authors.forename: Georgios N
authors.initials: GN
authors.lastname: Belibasakis
authors.forename: Durga
authors.initials: D
authors.lastname: Reddi
authors.forename: Nagihan
authors.initials: N
authors.lastname: Bostanci
mesh_terms: D001862:Bone Resorption; D015232:Dinoprostone / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D006801:Humans; D007249:Inflammation; D019169:Jurkat Cells; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D053244:Osteoprotegerin / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*; D053245:RANK Ligand / Q000235:genetics* / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D053244:Osteoprotegerin; D053245:RANK Ligand; D015232:Dinoprostone
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