Ultrasensitive optical detection of hydrogen peroxide by triggered activation of horseradish peroxidase.
Hydrogen peroxide is a very reactive byproduct of many metabolic pathways. We describe an ultra-sensitive colorimetric method to detect hydrogen peroxide based on the reconstitution of apo-horseradish peroxidase with the hemin derivative, hemin di(N,N'-acetyl-hydrazide). Oxidation of the latter by hydrogen peroxide yields hemin, which is able to reconstitute apo-horseradish peroxidase, forming an active peroxidase. We have also applied this analyte-triggered reconstitution of horseradish peroxidase to detect the activity of the enzyme glucose oxidase.
Virel
Ana
A
CIC biomaGUNE, Parque Tecnologico de San Sebastian, Paseo Miramon 182, 20009 San Sebastian, Spain.
Saa
Laura
L
Köster
Stephan David
SD
Pavlov
Valeri
V
D003124:Colorimetry / Q000379:methods*; D005949:Glucose Oxidase / Q000378:metabolism; D006427:Hemin / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis*; D010084:Oxidation-Reduction
D006427:Hemin; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase