Siliceous mesocellular foam for high-performance liquid chromatography: Effect of morphology and pore structure.
Spherical siliceous mesocellular foam (MCF) particles with an average particle size of 4.8 microm have been successfully prepared. These spherical particles were tailored in pore sizes and surface areas. They were functionalized with C(8) or C(18) groups, and applied towards reversed phase high-performance liquid chromatography (HPLC) column separations. Their high surface areas gave rise to very good retention characteristics, as illustrated in the separation of a series of alkylbenzene solutes with increasing chain length. The highly interconnected porous structure and ultralarge pore size of MCF allowed the columns to be used at high flow rates without much loss in column efficiency. The column efficiency and peak symmetry were further improved by eliminating the micropores of the stationary phase. The reversed phase column packed with C(18)-modified spherical MCF particles provided for excellent separation of different deoxynucleosides, illustrating the broad applicability of these materials due to their controlled pore size.
Han
Yu
Y
Institute of Bioengineering and Nanotechnology, The Nanos, Singapore 138669, Singapore.
Lee
Su Seong
SS
Ying
Jackie Y
JY
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D010316:Particle Size; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*
D012117:Resins, Synthetic; D058428:Silica Gel; D012822:Silicon Dioxide