Utility of a Luminex-based assay for multiplexed, rapid species identification of Candida isolates from an ongoing candidemia surveillance.
A Candida-specific Luminex-based assay with 11 probes was employed for multiplexed, rapid identification of 1182 Candida sp. isolates that were received as part of an ongoing population-based surveillance. All the Candida isolates were previously identified by a combination of methods, including phenotype and sequence analysis. Results showed that the Luminex assay was an attractive alternative to reference methods, as it is rapid, yields correct species identification, and is user friendly.
Deak
Eszter
E
Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Etienne
Kizee A
KA
Lockhart
Shawn R
SR
Gade
Lalitha
L
Chiller
Tom
T
Balajee
S Arunmozhi
SA
D002175:Candida / Q000145:classification* / Q000235:genetics; D002177:Candidiasis / Q000382:microbiology*; D015342:DNA Probes / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods*
D015342:DNA Probes; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer