title: Immortalized mouse floxed Bmp2 dental papilla mesenchymal cell lines preserve odontoblastic phenotype and respond to BMP2.
abstract: Bone morphogenetic protein 2 (Bmp2) is essential for odontogensis and dentin mineralization. Generation of floxed Bmp2 dental mesenchymal cell lines is a valuable application for studying the effects of Bmp2 on dental mesenchymal cell differentiation and its signaling pathways during dentinogenesis. Limitation of the primary culture of dental mesenchymal cells has led to the development of cell lines that serve as good surrogate models for the study of dental mesenchymal cell differentiation into odontoblasts and mineralization. In this study, we established and characterized immortalized mouse floxed Bmp2 dental papilla mesenchymal cell lines, which were isolated from 1st mouse mandibular molars at postnatal day 1 and immortalized with pSV40 and clonally selected. These transfected cell lines were characterized by RT-PCR, immunohistochemistry, and analyzed for alkaline phosphatase activity and mineralization nodule formation. One of these immortalized cell lines, iBmp2-dp, displayed a higher proliferation rate, but retained the genotypic and phenotypic characteristics similar to primary cells as determined by expression of tooth-specific markers as well as demonstrated the ability to differentiate and form mineralized nodules. In addition, iBmp2-dp cells were inducible and responded to BMP2 stimulation. Thus, we for the first time described the establishment of an immortalized mouse floxed Bmp2 dental papilla mesenchyma cell line that might be used for studying the mechanisms of dental cell differentiation and dentin mineralization mediated by Bmp2 and other growth factor signaling pathways.
authors.affiliation: Department of Pediatric Dentistry, Dental School, The University of Texas Health Science Center, San Antonio, Texas 78229-3900, USA.
authors.forename: Li-an
authors.initials: LA
authors.lastname: Wu
authors.forename: Junsheng
authors.initials: J
authors.lastname: Feng
authors.forename: Lynn
authors.initials: L
authors.lastname: Wang
authors.forename: Yan-dong
authors.initials: YD
authors.lastname: Mu
authors.forename: Andrew
authors.initials: A
authors.lastname: Baker
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Donly
authors.forename: Jelica
authors.initials: J
authors.lastname: Gluhak-Heinrich
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Harris
authors.forename: Mary
authors.initials: M
authors.lastname: MacDougall
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism*; D002113:Calcification, Physiologic; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D048430:Cell Shape; D003771:Dental Papilla / Q000166:cytology* / Q000502:physiology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D009804:Odontoblasts / Q000166:cytology* / Q000502:physiology*; D010641:Phenotype
chemical_list: D015415:Biomarkers; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2
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