title: Effect of storage conditions on prothrombin time, activated partial thromboplastin time and fibrinogen concentration on canine plasma samples.
abstract: The present study was to assess the effect of storage conditions on prothrombin time (PT), activated partial thromboplastin time (aPTT) and fibrinogen concentration in blood samples of healthy dogs. Thirty-five dogs of various breeds were included in the study. Citrated blood samples were obtained and plasma was divided into four aliquots to assess selected clotting parameters by means of a coagulometer. The first aliquot was analysed within 1 h after collection, while the remaining 3 were stored at 8 degrees C for 4, 8 and 24 h, respectively. One-way repeated measures analysis of variance documented a significant decreasing effect on PT at 24 h compared to 8 h and on fibrinogen concentration after 8 and 24 h compared to sampling time and at 4 and 24 h compared to 8 h post sampling. In conclusion, the results of this study indicate that only fibrinogen appears prone to significant decrease. In fact, aPTT is not substantially affected by refrigeration for at least 24 h post sampling and PT showed a statistical difference that does not necessary indicate biological significance as the results obtained were within reference intervals for the dog.
authors.affiliation: Dipartimento di Scienze Sperimentali e Biotecnologie Applicate, Sezione di Fisiologia Applicata ed Etologia Comparata, Facoltà di Medicina Veterinaria, Università degli Studi di Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy. giuseppe.piccione@unime.it
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Piccione
authors.forename: Stefania
authors.initials: S
authors.lastname: Casella
authors.forename: Claudia
authors.initials: C
authors.lastname: Giannetto
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Giudice
mesh_terms: D000818:Animals; D001793:Blood Preservation / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000097:blood*; D005340:Fibrinogen / Q000032:analysis*; D010314:Partial Thromboplastin Time / Q000662:veterinary*; D010949:Plasma / Q000737:chemistry; D011517:Prothrombin Time / Q000662:veterinary*; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*; D013997:Time Factors
chemical_list: D005340:Fibrinogen
references.citation: Vet Surg. 2001 Jan-Feb;30(1):97-104
references.pmid: 11172465
references.citation: Clin Chem Lab Med. 1998 Jun;36(7):459-62
references.pmid: 9746270
references.citation: Clin Chim Acta. 2000 Oct;300(1-2):13-21
references.pmid: 10958859
references.citation: Semin Vet Med Surg Small Anim. 1992 Nov;7(4):315-22
references.pmid: 1470774
references.citation: Transfusion. 1999 May;39(5):488-91
references.pmid: 10335998
references.citation: Res Vet Sci. 2008 Aug;85(1):166-70
references.pmid: 17998140
references.citation: Thromb Res. 2009 May;124(1):121-6
references.pmid: 19128820
references.citation: Can J Vet Res. 1999 Jul;63(3):216-20
references.pmid: 10480465
references.citation: Vet Clin North Am Small Anim Pract. 1988 Jan;18(1):87-93
references.pmid: 3282387
references.citation: Thromb Res. 2002 Sep 1;107(5):263-9
references.pmid: 12479888
references.citation: J Vet Diagn Invest. 2000 Sep;12(5):433-7
references.pmid: 11021430
references.citation: J Vet Intern Med. 2006 Nov-Dec;20(6):1319-26
references.pmid: 17186844
references.citation: J Vet Diagn Invest. 2008 Nov;20(6):774-9
references.pmid: 18987227
references.citation: Can Vet J. 1991 Mar;32(3):173-5
references.pmid: 17423756
references.citation: Vet Clin Pathol. 2006 Jun;35(2):204-7
references.pmid: 16783714
references.citation: J Am Vet Med Assoc. 1981 Jul 15;179(2):151-4
references.pmid: 7263467