Extracellular purine metabolism in blood vessels (Part II): Activity of ecto-enzymes in blood vessels of patients with abdominal aortic aneurysm.
Both platelet aggregation and high blood pressure are associated with development of atherosclerosis. Among other factors that modulate platelet aggregation and blood pressure, extracellular purines (e-purines) influence these processes via purinoceptors P1 and P2 for which they are natural ligands. We hypothesized that ecto-enzymes such as nucleoside triphosphate diphosphohydrolases (NTPDases), adenylate kinase, 5'-nucleotidase, and adenosine deaminase that regulate the level of e-purines may be involved in the development of atherosclerosis. The enzymatic assays were performed either on the fragments of human abdominal aortas obtained after death or on abdominal aneurysm samples collected during surgery. The substrates and products such as adenine nucleosides and nucleotides were analyzed using reverse phase high-performance liquid chromatography (HPLC) method. Here, we estimated and demonstrated the activities of these ecto-enzymes in the patients with atherosclerosis or atherosclerosis-like diseases such as abdominal aneurysm, myocardial infarction, or Leriche syndrome (LS) with worse thrombosis of extremities. In particular, we noticed reduction in activity of NTPDase1(app), NTPDase2(app), ecto-adenylate kinase( app), and ecto-adenosine deaminase(app); however, ecto-5'-nucleotidase(app) that hydrolyzed e-adenosine monophosphate (e-AMP) into e-adenosine did not show any significant changes. This led us to suggest that alteration of the activity of examined ecto-enzymes is responsible for the development of atherosclerosis or atherosclerosis-like diseases.
Lecka
Joanna
J
Department of Biochemistry, Nicolaus Copernicus University, Torun, Poland. joanna.lecka@crchul.ulaval.ca
Bloch-Boguslawska
Elzbieta
E
Molski
Stanislaw
S
Komoszynski
Michal
M
D015720:5'-Nucleotidase / Q000378:metabolism; D000243:Adenosine Deaminase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000263:Adenylate Kinase / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D017544:Aortic Aneurysm, Abdominal / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D001081:Apyrase / Q000378:metabolism; D050197:Atherosclerosis / Q000201:enzymology / Q000378:metabolism; D001808:Blood Vessels / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D002470:Cell Survival / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D007925:Leriche Syndrome / Q000097:blood / Q000201:enzymology / Q000378:metabolism*; D011687:Purines / Q000097:blood* / Q000378:metabolism*
D015703:Antigens, CD; D011687:Purines; D000263:Adenylate Kinase; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase; D000251:Adenosine Triphosphatases; C027201:ectoATPase; D001081:Apyrase; C067869:CD39 antigen