title: Polymorphism Trp64Arg of beta 3 adrenoreceptor gene: allelic frequencies and influence on insulin resistance in a multicenter study of Castilla-León.
abstract: OBJECTIVE
  The genetic variant (Trp64Arg) is a missense mutation located within the beta3 adrenoreceptor (Beta3AR). The aim of our study was to investigate the influence of Trp64Arg polymorphism in the Beta3AR gene on insulin resistance in obese patients and the allelic distribution of this polymorphismin a geographic area of Spain.
  
  
  METHODS
  A population of 264 obese patients was analyzed. A bioimpedance, blood pressure, an assessment of nutritional intake, and biochemical parameters were measured. The beta 3 adrenoreceptor gene polymorphism(Trp64Arg) was genotyped.
  
  
  RESULTS
  Two hundred and twenty six patients (77 males/149 females) (85.6%) had the genotype Trp64/Trp64 (wild type group) with and average age of 41.12 +/- 13.1 years and 38 patients (16 males/22 females) Trp64/Arg64 (14.4%) (mutant type group) with an average age of 40.5 +/- 12.7 years. High frequencies of Arg64 allele were observed in Salamanca and Valladolid. In the mutant type group, HOMA (3.75 +/- 2.77 vs 5.27 +/- 5.4; p < 0.05) was higher than wild type group.
  
  
  CONCLUSIONS
  The finding of this study is the association of the Trp64/Arg64 Beta3AR with higher levels of HOMA. Frequencies of this polymorphism are different among geographic areas.
authors.affiliation: Institute of Endocrinology and Nutrition, Medicine School, University of Valladolid, Spain. dadluis@yahoo.es
authors.forename: D A
authors.initials: DA
authors.lastname: de Luis
authors.forename: M
authors.initials: M
authors.lastname: Ballesteros
authors.forename: E
authors.initials: E
authors.lastname: Ruiz
authors.forename: C
authors.initials: C
authors.lastname: Muñoz
authors.forename: A
authors.initials: A
authors.lastname: Penacho
authors.forename: P
authors.initials: P
authors.lastname: Iglesias
authors.forename: A
authors.initials: A
authors.lastname: López Guzmán
authors.forename: A
authors.initials: A
authors.lastname: Maldonado
authors.forename: M
authors.initials: M
authors.lastname: Cordero
authors.forename: L
authors.initials: L
authors.lastname: San Martín
authors.forename: V
authors.initials: V
authors.lastname: Puigdevall
authors.forename: E
authors.initials: E
authors.lastname: Romero
authors.forename: M
authors.initials: M
authors.lastname: González Sagrado
authors.forename: O
authors.initials: O
authors.lastname: Izaola
authors.forename: R
authors.initials: R
authors.lastname: Conde
mesh_terms: D000328:Adult; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics*; D013030:Spain
chemical_list: D022702:Receptors, Adrenergic, beta-3