[Cloning of PCR-copies of Streptomyces coelicolor A3(2) chromosome fragment].
Copies of chromosome Streptomyces coelicolor A3(2) fragment (gene coding polyketide syntase of III type) were obtained to use polymerase cycle reaction. Amplified DNA was cloned in shuttle vector pWHM4 on sites for HindIII and EcoRI. The restriction analysis of plasmid DNAs of transformants has demonstrated that molecular size of cloned DNA fragment was 1134 bp.
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D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D048630:Polyketide Synthases / Q000235:genetics; D016133:Polymerase Chain Reaction; D048372:Streptomyces coelicolor / Q000201:enzymology / Q000235:genetics*
D004269:DNA, Bacterial; D048630:Polyketide Synthases; D004262:DNA Restriction Enzymes