Abundance of some skeletal muscle mitochondrial proteins is associated with increased blood serum insulin in bovine fetuses.
The aim of this study was to investigate the evolution of the abundance of cytochrome oxidase c subunit IV (NCOIV) and beta subunit of ATP synthase (β-ATP) during the last third of gestation in bovine skeletal muscles. Semitendinosus, longissimus thoracis and rectus abdominis muscles were chosen for the immunoblotting of the respective protein levels. Muscle and blood samples from bovine fetuses of randomly selected breeds were collected at 180, 210, and 260 days post-conception (dpc). The muscle tissue expressions of NCOIV, β-ATP were compared to blood glucose and insulin. At 260 dpc, protein levels of NCOIV raised in skeletal muscles. Additionally, β-ATP in semitendinosus and longissimus thoracis were elevated and paralleled by higher concentrations of blood serum insulin. It corroborates our previous observations indicating that accelerated metabolic differentiation of bovine skeletal muscles is associated with elevated blood insulin and occurs during the last trimester of gestation. Our observations point to the connection between insulin-sensitivity and the molecular mechanisms of mitochondrial contribution to ontogenesis of skeletal muscles.
Pajak
Beata
B
Mossakowski Medical Research Center, Polish Academy of Sciences, Pawinskiego 5, 02-106 Warsaw, Poland.
Pawlikowska
Patrycja
P
Cassar-Malek
Isabelle
I
Picard
Brigitte
B
Hocquette
Jean-François
JF
Orzechowski
Arkadiusz
A
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002417:Cattle; D003576:Electron Transport Complex IV / Q000378:metabolism / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D005333:Fetus / Q000378:metabolism* / Q000502:physiology; D015151:Immunoblotting / Q000662:veterinary; D007328:Insulin / Q000097:blood*; D008931:Mitochondria, Muscle / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism / Q000502:physiology; D018482:Muscle, Skeletal / Q000196:embryology; D011247:Pregnancy; D021122:Protein Subunits
D001786:Blood Glucose; D007328:Insulin; D021122:Protein Subunits; D003576:Electron Transport Complex IV; D025261:Mitochondrial Proton-Translocating ATPases