Evidence for the involvement of Lfc and Tctex-1 in axon formation.
RhoA and Rac play key and opposite roles during neuronal polarization. We now show that Lfc, a guanosine nucleotide exchange factor (GEF), localizes to the Golgi apparatus and growth cones of developing neurons and negatively regulates neurite sprouting and axon formation through a Rho signaling pathway. Tctex-1, a dynein light chain implicated in axon outgrowth by modulating actin dynamics and Rac activity, colocalizes and physically interacts with Lfc, thus inhibiting its GEF activity, decreasing Rho-GTP levels, and functionally antagonizing Lfc during neurite formation.
Conde
Cecilia
C
Instituto de Investigación Médica Mercedes y Martín Ferreyra-Consejo Nacional de Investigaciones Científicas y Técnicas, 5016 Cordoba, Argentina.
Arias
Cristina
C
Robin
Maria
M
Li
Aiqun
A
Saito
Masaki
M
Chuang
Jen-Zen
JZ
Nairn
Angus C
AC
Sung
Ching-Hwa
CH
Cáceres
Alfredo
A
D000818:Animals; D001369:Axons / Q000502:physiology*; D016466:CHO Cells; D049108:Cell Enlargement; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004398:Dyneins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D020439:Growth Cones / Q000502:physiology; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000502:physiology; D051379:Mice; D016501:Neurites / Q000502:physiology; D051381:Rats; D015398:Signal Transduction; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
C533388:Dynlt1 protein, rat; D020662:Guanine Nucleotide Exchange Factors; C549918:Lfc protein, rat; D004398:Dyneins; D020742:rhoA GTP-Binding Protein
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