DNA typing for the identification of old skeletal remains from Korean War victims.
The identification of missing casualties of the Korean War (1950-1953) has been performed using mitochondrial DNA (mtDNA) profiles, but recent advances in DNA extraction techniques and approaches using smaller amplicons have significantly increased the possibility of obtaining DNA profiles from highly degraded skeletal remains. Therefore, 21 skeletal remains of Korean War victims and 24 samples from biological relatives of the supposed victims were selected based on circumstantial evidence and/or mtDNA-matching results and were analyzed to confirm the alleged relationship. Cumulative likelihood ratios were obtained from autosomal short tandem repeat, Y-chromosomal STR, and mtDNA-genotyping results, and mainly confirmed the alleged relationship with values over 10⁵. The present analysis emphasizes the value of mini- and Y-STR systems as well as an efficient DNA extraction method in DNA testing for the identification of old skeletal remains.
Lee
Hwan Young
HY
Department of Forensic Medicine and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodaemun-Gu, Seoul 120-752, Korea.
Kim
Na Young
NY
Park
Myung Jin
MJ
Sim
Jeong Eun
JE
Yang
Woo Ick
WI
Shin
Kyoung-Jin
KJ
D041322:Chromosomes, Human, Y; D022801:Complementarity Determining Regions / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D047869:Korean War; D016013:Likelihood Functions; D008297:Male; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences
D022801:Complementarity Determining Regions; D017931:DNA Primers; D004272:DNA, Mitochondrial