title: DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases.
abstract: DNA-scaffolded oligodeoxyriboside fluorophores (ODFs) were used as the reporters in turn-on sensing of enzymatic bond-cleaving activity. A tetramer ODF of pyrene deoxynucleosides displayed high quenching efficiency when conjugated via ester linkages with a dabcyl quencher, and yielded large signal increases with several enzymes in vitro and in intact human cells.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
authors.forename: Nan
authors.initials: N
authors.lastname: Dai
authors.forename: Yin Nah
authors.initials: YN
authors.lastname: Teo
authors.forename: Eric T
authors.initials: ET
authors.lastname: Kool
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry; D003853:Deoxyribonucleosides / Q000737:chemistry* / Q000378:metabolism; D004950:Esterases / Q000032:analysis / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008049:Lipase / Q000032:analysis / Q000378:metabolism*; D015394:Molecular Structure
chemical_list: D003853:Deoxyribonucleosides; D005456:Fluorescent Dyes; D004247:DNA; D004950:Esterases; D008049:Lipase
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