Date seed oil inhibits hydrogen peroxide-induced oxidative stress in human epidermal keratinocytes.
BACKGROUND
Oxidative stress has been implicated in various skin diseases through the generation of reactive oxygen species and the depletion of endogenous antioxidant systems. The administration of antioxidants is reportedly helpful, notably to enhance the healing process. To protect the skin against oxidative damages, we have studied the effect of new oil: "date seed oil" (DSO). This oil, may serve as a potential source of natural antioxidants such as phenols and tocopherols.


METHODS
Here, we report the protective effect of DSO against hydrogen peroxide (H(2)O(2))-induced oxidative stress in terms of lipid peroxidation, depletion of endogenous antioxidant defense enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) using normal human epidermal keratinocytes (NHEK).


RESULTS
In the investigated model system, DSO has significant chemoprotective effect, by inhibition of damage caused by H(2)O(2) compared with cells without such addition endowing with a radical scavenging ability. Treatment of NHEK with DSO inhibited H(2)O(2)-induced lipid peroxidation. In addition, this oil inhibited H(2)O(2)-induced depletion of antioxidant defense components, such as SOD, CAT and GPx.


CONCLUSIONS
Our findings demonstrate that DSO is an efficient extract that is able to prevent keratinocytes oxidative damage induced by H(2)O(2) exposure and may thus be a potential promising candidate, as a chemopreventive agent, in the development of keratinocytes-related pathologies.
Ines
Dammak
D
Unité de recherche, Pathologies Humaines et stress oxydatif, Institut Supérieur de Biotechnologie de Sfax, Sfax, Tunisie. inesdammak@yahoo.fr
Sonia
Boudaya
B
Fatma
Ben Abdallah
BA
Souhail
Besbes
B
Hamadi
Attia
A
Hamida
Turki
T
Basma
Hentati
H
D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D028023:Arecaceae / Q000737:chemistry*; D002374:Catalase / Q000032:analysis; D004817:Epidermis / Q000187:drug effects* / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000032:analysis; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D015603:Keratinocytes / Q000187:drug effects* / Q000201:enzymology; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010938:Plant Oils / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D013482:Superoxide Dismutase / Q000032:analysis
D000975:Antioxidants; D010938:Plant Oils; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase