title: Protective effect of green tea (-)-epigallocatechin-3-gallate against the monoamine oxidase B enzyme activity increase in adult rat brains.
abstract: OBJECTIVE
  Monoamine oxidase B (MAO-B) levels were observed increasing during aging in rat brains. (-)-Epigallocatechin-3-gallate (EGCG) is the major polyphenolic constituent in green tea. The objective of the present study was to investigate the EGCG compound for its effect on preventing an increase in MAO-B activity in rat brains. The total antioxidant capacity and lipid peroxidation of rats were also assessed.
  
  
  METHODS
  Rats were assigned to three groups: Control, VE (α-tocopherol), and EGCG. Twenty-four male Long-Evans rats were fed normal diets for a total of 11 wk and test diets for a total of 12 wk. The serum analysis, serum total antioxidant capacity, tissue lipid peroxidation, and monoamine oxidase B enzyme activity were measured. The differences between the groups and between the control and experimental groups were analyzed. The correlation among the experimental results was also analyzed.
  
  
  RESULTS
  The serum total antioxidant capacity of the EGCG group was higher than that observed in the Control and VE groups. In rat brains and livers, the lipid peroxidation levels were lower in the VE and EGCG groups compared with Control groups. EGCG and VE groups showed lower MAO-B enzyme activity in rat brains compared with Control groups. In contrast to the brain findings, there were no significant differences in the MAO-B enzyme activity among groups in rat livers.
  
  
  CONCLUSIONS
  The present study first indicates that EGCG supplementation was able to execute a tissue-selective decrease in the brain MAO-B enzyme activity in adult rats, in which it was actualized by way of preventing physiological peroxidation.
authors.affiliation: Department of Food and Nutrition, Chung Hwa University of Medical Technology, Taiwan, ROC.
authors.forename: Shyh-Mirn
authors.initials: SM
authors.lastname: Lin
authors.forename: Shih-Wei
authors.initials: SW
authors.lastname: Wang
authors.forename: Su-Chen
authors.initials: SC
authors.lastname: Ho
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Tang
mesh_terms: D000375:Aging / Q000097:blood / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000627:therapeutic use*; D001921:Brain / Q000201:enzymology* / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D019587:Dietary Supplements; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism*; D019636:Neurodegenerative Diseases / Q000517:prevention & control; D009474:Neurons / Q000201:enzymology / Q000378:metabolism; D009928:Organ Specificity; D011897:Random Allocation; D051381:Rats; D020318:Rats, Long-Evans; D013662:Tea / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D013662:Tea; D017392:Thiobarbituric Acid Reactive Substances; D002392:Catechin; C045651:epigallocatechin gallate; D008995:Monoamine Oxidase