Nuclear-export-signal-dependent protein translocation of dUTPase encoded by Singapore grouper iridovirus.
The dUTPase is a ubiquitous and crucial enzyme responsible for regulating cellular levels of dUTP. In the present study, the expression pattern and translocation of a dUTPase homolog encoded by Singapore grouper iridovirus (SGIV) were elucidated. The SGIV ORF049R encodes a dUTPase homolog, which is a peptide of 155 amino acids that contains five conserved motifs. The temporal expression pattern during infection in vitro revealed that the SGIV dUTPase was an early transcript. A leucine-rich nuclear export signal (NES) at the C-terminus was predicted using CBS Online Servers. Subcellular location analysis showed that SGIV dUTPase is a cytoplasmic protein. Site-direct mutagenesis by overlap extension-PCR indicated that the NES is crucial for the translocation of SGIV dUTPase from the nucleus to the cytoplasm. We have discovered for the first time that the NES-dependent translocation of dUTPase is different for SGIV than for members of other species, which depend on a nuclear localization signal. These results provide new insights into the pathogenesis of fish iridoviruses.
Gong
Jie
J
State Key Laboratory of Biocontrol, School of Life Sciences, SunYat-Sen (Zhongshan) University, Guangzhou 510275, People's Republic of China.
Huang
You-Hua
YH
Huang
Xiao-Hong
XH
Zhang
Rong
R
Qin
Qi-Wei
QW
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D019295:Computational Biology; D005399:Fishes; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D017994:Iridovirus / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011755:Pyrophosphatases / Q000378:metabolism*; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D014764:Viral Proteins; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase