title: Extended-spectrum beta-lactamase-producing Salmonella enterica serovar Oranienburg (CTX-M-2 group) in a pediatric hospital in Tucumán, Argentina.
abstract: BACKGROUND
  Salmonella sp infections have been reported over recent years in hospitals in Argentina and other countries due to multiresistant strains. The aim of this study was to characterize the extended-spectrum beta-lactamases in third-generation cephalosporin-resistant strains of Salmonella enterica serovar Oranienburg.
  
  
  METHODS
  We studied 60 strains isolated from children with gastroenteritis and/or extraintestinal complications. The antibiotic susceptibility patterns of the isolates were analyzed and the beta-lactamases were characterized using phenotyping and genotyping methods.
  
  
  RESULTS
  All the strains were resistant to ampicillin, cefotaxime, cefepime and aztreonam and partially susceptible to ceftazidime, thus corresponding well with the resistance phenotype conferred by CTX-M-type beta-lactamases. An isoelectric point enzyme (pI = 7.9) was detected in all of the strains, and this was confirmed by PCR as a member of the CTX-M-2 group.
  
  
  CONCLUSIONS
  This is the first report of Salmonella enterica serovar Oranienburg producing beta-lactamases of the CTX-M-2 group in a pediatric hospital in Tucumán, Argentina.
authors.affiliation: Instituto de Microbiología Dr. Luis C Verna, Facultat de Bioquímica, Química, Farmacia y Biotecnología, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina.
authors.forename: María Angela
authors.initials: MA
authors.lastname: Jure
authors.forename: Olga
authors.initials: O
authors.lastname: Aulet
authors.forename: Ana
authors.initials: A
authors.lastname: Trejo
authors.forename: Marta
authors.initials: M
authors.lastname: Castillo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001118:Argentina; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics; D005759:Gastroenteritis / Q000382:microbiology; D005838:Genotype; D006776:Hospitals, Pediatric; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D016133:Polymerase Chain Reaction; D019779:Salmonella enterica / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C071167:beta-lactamase CTX-2; D001618:beta-Lactamases