title: Capillary electrophoretic determination of selected phenolic compounds in humic substances of well waters and fertilizers.
abstract: Humic substances (HS) from well waters, fertilizers, and synthetic phenolic polymers were characterized by elemental and UV-VIS spectroscopic analyses. Capillary zone electrophoresis (CZE) with UV absorption detection was used to analyze the lignin-derived phenolic distribution in the degradation residues after alkaline CuO oxidation of HS samples. Eleven phenols with p-acetyl, vanillyl and syringyl substituents were selected to optimize the CZE parameters. For well waters and fertilizers, the content of phenolic fragments was in agreement with the findings of the elemental and spectroscopic measurements. Additionally, parameters derived from the vanillic acid/vanilline, syringyl acid/syringaldehyde, p-hydroxyl/vanillyl and syringyl/vanillyl ratios matched analogous studies on dissolved organic matter from natural waters and on humic acids from terrestrial substances. The amount of phenolic monomer bonded within two synthetic HS polymers was found to be 25.9% protocatechuic acid and 71.3% gallic acid.
authors.affiliation: Department of Family Medicine, E-Da Hospital, No. 1, Yi-Da Rd, Jiau-Shu Tsuen, Yan-Chau Shiang, Kaohsiung County 42445, Taiwan.
authors.forename: Mei-Ying
authors.initials: MY
authors.lastname: Chen
authors.forename: Yan-Zin
authors.initials: YZ
authors.lastname: Chang
authors.forename: Fung-Jou
authors.initials: FJ
authors.lastname: Lu
authors.forename: Jian-Lian
authors.initials: JL
authors.lastname: Chen
mesh_terms: D000042:Absorption; D001547:Benzaldehydes / Q000032:analysis; D003300:Copper / Q000737:chemistry; D019075:Electrophoresis, Capillary; D005308:Fertilizers / Q000032:analysis*; D005707:Gallic Acid / Q000032:analysis; D006812:Humic Substances / Q000032:analysis*; D062385:Hydroxybenzoates / Q000032:analysis; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis*; D011108:Polymers / Q000032:analysis* / Q000138:chemical synthesis; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014641:Vanillic Acid / Q000032:analysis; D014881:Water Supply / Q000032:analysis*
chemical_list: D001547:Benzaldehydes; D005308:Fertilizers; D006812:Humic Substances; D062385:Hydroxybenzoates; D010636:Phenols; D011108:Polymers; C069665:syringaldehyde; C009091:protocatechuic acid; D005707:Gallic Acid; D003300:Copper; D014641:Vanillic Acid; C030973:cupric oxide