title: The decapping activator HPat a novel factor co-purifying with GW182 from Drosophila cells.
abstract: miRNAs post-transcriptionally regulate gene expression in many eukaryotes and thereby affect a wide range of biological processes. GW182 is a key factor in translation repression and mRNA degradation by miRNAs. In this study we investigate the potential interaction of GW182 and translation or mRNA degradation factors in Drosophila S2 cells. We have identified the decapping activator HP at as a novel factor co-purifying with GW182. Furthermore, we show that the C-terminal domain of GW182, important for gene silencing, is sufficient to form a complex with HP at. Our findings implicate a potential interaction of the miRNA effector component GW182 with the decapping machinery.
authors.affiliation: University of Vienna, Department of Biochemistry and Cell Biology, Vienna, Austria.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Jäger
authors.forename: Silke
authors.initials: S
authors.lastname: Dorner
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D053487:DEAD-box RNA Helicases / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins / Q000302:isolation & purification / Q000378:metabolism*; D047468:Immunoprecipitation; D008954:Models, Biological; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000502:physiology; D012315:RNA Caps / Q000378:metabolism; D020871:RNA Stability / Q000502:physiology; D016601:RNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; C513405:Gw protein, Drosophila; C549399:Patr-1 protein, Drosophila; D012315:RNA Caps; D016601:RNA-Binding Proteins; C532748:Me31B protein, Drosophila; D053487:DEAD-box RNA Helicases
references.citation: Nature. 2003 Dec 4;426(6966):579-84
references.pmid: 14654845
references.citation: Nat Struct Mol Biol. 2008 Apr;15(4):346-53
references.pmid: 18345015
references.citation: Curr Biol. 2000 Sep 21;10(18):1147-50
references.pmid: 10996799
references.citation: J Biol Chem. 2010 Apr 30;285(18):13599-606
references.pmid: 20181956
references.citation: Mol Cell Biol. 2010 Mar;30(6):1486-94
references.pmid: 20065043
references.citation: RNA. 2005 Nov;11(11):1640-7
references.pmid: 16177138
references.citation: Genes Dev. 2007 Aug 1;21(15):1857-62
references.pmid: 17671087
references.citation: RNA. 2001 Dec;7(12):1717-27
references.pmid: 11780629
references.citation: Mol Cell Biol. 2008 Feb;28(4):1298-312
references.pmid: 18086885
references.citation: RNA. 2009 May;15(5):794-803
references.pmid: 19304924
references.citation: Nat Rev Genet. 2008 Feb;9(2):102-14
references.pmid: 18197166
references.citation: RNA. 2010 Jan;16(1):239-50
references.pmid: 19934229
references.citation: EMBO J. 1998 Dec 15;17(24):7480-9
references.pmid: 9857202
references.citation: Mol Cell. 2008 Jan 18;29(1):1-7
references.pmid: 18206964
references.citation: Cell. 2005 Sep 23;122(6):875-86
references.pmid: 16179257
references.citation: RNA. 2003 Oct;9(10):1171-3
references.pmid: 13130130
references.citation: Mol Cell. 2009 Sep 24;35(6):868-80
references.pmid: 19716330
references.citation: RNA. 2008 Dec;14(12):2580-96
references.pmid: 18978028
references.citation: Mol Cell Biol. 2009 Dec;29(23):6220-31
references.pmid: 19797087
references.citation: Nat Struct Mol Biol. 2010 Feb;17(2):238-40
references.pmid: 20098421
references.citation: J Biol Chem. 1984 Oct 10;259(19):11930-5
references.pmid: 6566675
references.citation: RNA. 2009 Jun;15(6):1067-77
references.pmid: 19383769
references.citation: Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):16961-6
references.pmid: 16287976
references.citation: Biochim Biophys Acta. 2007 Dec;1773(12):1786-92
references.pmid: 17936923
references.citation: Nature. 2008 Sep 4;455(7209):58-63
references.pmid: 18668040
references.citation: Mol Cell. 1998 Jul;2(1):135-40
references.pmid: 9702200
references.citation: Science. 2006 Apr 7;312(5770):75-9
references.pmid: 16484454
references.citation: PLoS One. 2009 Aug 27;4(8):e6783
references.pmid: 19710908
references.citation: EMBO Rep. 2007 Nov;8(11):1052-60
references.pmid: 17932509
references.citation: Genes Dev. 2006 Jul 15;20(14):1885-98
references.pmid: 16815998
references.citation: Mol Cell. 2009 Apr 10;34(1):58-67
references.pmid: 19268617
references.citation: Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4034-9
references.pmid: 16495412
references.citation: Mol Cell. 2009 Sep 24;35(6):881-8
references.pmid: 19782035
references.citation: Nature. 2007 Jun 14;447(7146):823-8
references.pmid: 17507929
references.citation: Genes Dev. 2007 Oct 15;21(20):2558-70
references.pmid: 17901217
references.citation: EMBO J. 2000 Apr 3;19(7):1661-71
references.pmid: 10747033
references.citation: Nature. 2000 Mar 30;404(6777):515-8
references.pmid: 10761922
references.citation: Cell. 2005 Aug 26;122(4):553-63
references.pmid: 16122423
references.citation: Nature. 2008 Sep 4;455(7209):64-71
references.pmid: 18668037
references.citation: Nat Struct Mol Biol. 2009 Nov;16(11):1160-6
references.pmid: 19838187
references.citation: Mol Cell Biol. 2007 Jun;27(11):3970-81
references.pmid: 17403906
references.citation: Mol Cell Biol. 2000 Aug;20(16):5939-46
references.pmid: 10913177
references.citation: RNA. 2009 Jan;15(1):21-32
references.pmid: 19029310
references.citation: RNA. 2009 May;15(5):781-93
references.pmid: 19304925