title: Cloning, expression, and induction by 17-beta estradiol (E2) of a vitellogenin gene in the white cloud mountain minnow Tanichthys albonubes.
abstract: Vitellogenins (Vtgs), the precursors for the yolk proteins, are very important for the embryonic development of teleosts, and have also been studied extensively as biomarkers for environmental estrogenic mimics. The cDNA for a Vtg was isolated from the liver of the female white cloud mountain minnow (Tanichthys albonubes) by 3'- and 5'-RACE methods. It is 4,171 bp in full length, and encodes a putative protein of 1,326 amino acids. This putative Vtg, designated as wcmmVtg, contains complete portions of LVI and PV, but lacks the C-terminal half of LVII and thus belongs to type I vitellogenin. In addition to the liver of the female fish, wcmmVtg was also shown to be expressed in the ovary. During ovarian development, the mRNA expression of wcmmVtg in both the liver and ovary was continuously increased from the previtellogenic to late vitellogenic stages, but then decreased significantly at post-spawning stage. In the male fish, expression of wcmmVtg mRNA was induced in the liver by treatment with E2 (10 and 100 ng/l) for 14 days. These results suggest that the Vtg originated from the ovary of the white cloud mountain minnow may also contribute to the accumulation of yolk proteins during oocyte growth, and that the male white cloud mountain minnow is sensitive to the estrogenic treatment in terms of Vtg mRNA expression, which could also be applied to monitor the environmental estrogenic mimics.
authors.affiliation: Institute of Aquatic Economic Animals, School of Life Sciences, Zhongshan (Sun Yat-Sen) University, Guangzhou 510275, People's Republic of China.
authors.forename: Ruilong
authors.initials: R
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhang
authors.forename: Yike
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhanqiang
authors.initials: Z
authors.lastname: Fang
authors.forename: Jianguo
authors.initials: J
authors.lastname: He
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhang
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Ma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D029941:Fish Proteins / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014819:Vitellogenins / Q000235:genetics*
chemical_list: D017931:DNA Primers; D029941:Fish Proteins; D012333:RNA, Messenger; D014819:Vitellogenins; D004958:Estradiol
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