title: Association study of 182 candidate genes in anorexia nervosa.
abstract: We performed association studies with 5,151 SNPs that were judged as likely candidate genetic variations conferring susceptibility to anorexia nervosa (AN) based on location under reported linkage peaks, previous results in the literature (182 candidate genes), brain expression, biological plausibility, and estrogen responsivity. We employed a case-control design that tested each SNP individually as well as haplotypes derived from these SNPs in 1,085 case individuals with AN diagnoses and 677 control individuals. We also performed separate association analyses using three increasingly restrictive case definitions for AN: all individuals with any subtype of AN (All AN: n = 1,085); individuals with AN with no binge eating behavior (AN with No Binge Eating: n = 687); and individuals with the restricting subtype of AN (Restricting AN: n = 421). After accounting for multiple comparisons, there were no statistically significant associations for any individual SNP or haplotype block with any definition of illness. These results underscore the importance of large samples to yield appropriate power to detect genotypic differences in individuals with AN and also motivate complementary approaches involving Genome-Wide Association (GWA) studies, Copy Number Variation (CNV) analyses, sequencing-based rare variant discovery assays, and pathway-based analysis in order to make up for deficiencies in traditional candidate gene approaches to AN.
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7160, USA.
authors.forename: Andrea Poyastro
authors.initials: AP
authors.lastname: Pinheiro
authors.forename: Cynthia M
authors.initials: CM
authors.lastname: Bulik
authors.forename: Laura M
authors.initials: LM
authors.lastname: Thornton
authors.forename: Patrick F
authors.initials: PF
authors.lastname: Sullivan
authors.forename: Tammy L
authors.initials: TL
authors.lastname: Root
authors.forename: Cinnamon S
authors.initials: CS
authors.lastname: Bloss
authors.forename: Wade H
authors.initials: WH
authors.lastname: Berrettini
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Schork
authors.forename: Walter H
authors.initials: WH
authors.lastname: Kaye
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Bergen
authors.forename: Pierre
authors.initials: P
authors.lastname: Magistretti
authors.forename: Harry
authors.initials: H
authors.lastname: Brandt
authors.forename: Steve
authors.initials: S
authors.lastname: Crawford
authors.forename: Scott
authors.initials: S
authors.lastname: Crow
authors.forename: Manfred M
authors.initials: MM
authors.lastname: Fichter
authors.forename: David
authors.initials: D
authors.lastname: Goldman
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Halmi
authors.forename: Craig
authors.initials: C
authors.lastname: Johnson
authors.forename: Allan S
authors.initials: AS
authors.lastname: Kaplan
authors.forename: Pamela K
authors.initials: PK
authors.lastname: Keel
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Klump
authors.forename: Maria
authors.initials: M
authors.lastname: La Via
authors.forename: James E
authors.initials: JE
authors.lastname: Mitchell
authors.forename: Michael
authors.initials: M
authors.lastname: Strober
authors.forename: Alessandro
authors.initials: A
authors.lastname: Rotondo
authors.forename: Janet
authors.initials: J
authors.lastname: Treasure
authors.forename: D Blake
authors.initials: DB
authors.lastname: Woodside
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000856:Anorexia Nervosa / Q000235:genetics*; D002032:Bulimia / Q000235:genetics; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D055815:Young Adult
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