Local and global mobility in the ClpA AAA+ chaperone detected by cryo-electron microscopy: functional connotations.
The ClpA chaperone combines with the ClpP peptidase to perform targeted proteolysis in the bacterial cytoplasm. ClpA monomer has an N-terminal substrate-binding domain and two AAA+ ATPase domains (D1 and D2). ClpA hexamers stack axially on ClpP heptamers to form the symmetry-mismatched protease. We used cryo-electron microscopy to visualize the ClpA-ATPgammaS hexamer, in the context of ClpAP complexes. Two segments lining the axial channel show anomalously low density, indicating that these motifs, which have been implicated in substrate translocation, are mobile. We infer that ATP hydrolysis is accompanied by substantial structural changes in the D2 but not the D1 tier. The entire N domain is rendered invisible by large-scale fluctuations. When deletions of 10 and 15 residues were introduced into the linker, N domain mobility was reduced but not eliminated and changes were observed in enzymatic activities. Based on these observations, we present a pseudo-atomic model of ClpAP holoenzyme, a dynamic proteolytic nanomachine.
Effantin
Grégory
G
Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Ishikawa
Takashi
T
De Donatis
Gian Marco
GM
Maurizi
Michael R
MR
Steven
Alasdair C
AC
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives; D000951:Antigens, Neoplasm; D020285:Cryoelectron Microscopy; D004250:DNA Topoisomerases, Type II; D004268:DNA-Binding Proteins; D049071:Endopeptidase Clp / Q000737:chemistry*; D010450:Endopeptidases / Q000378:metabolism*; D006868:Hydrolysis; D046911:Macromolecular Substances; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary
D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D018832:Molecular Chaperones; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate; D010450:Endopeptidases; D049071:Endopeptidase Clp; D004250:DNA Topoisomerases, Type II
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