[Peculiarities of surface-active trehalose mycolates synthesis of Rhodococcus erythropolis EK-1].
It has been established that activity of trehalosephosphate synthase (key enzyme of trehalosemycolates synthesis) under cultivation of Rhodococcus erythropolis EK-1 on the medium with n-hexadecane was 10 times higher, and amount of synthesized surface-active substances 4 times higher as compared to the growing of EK-1 strain on ethanol. Introduction of 0.2% of fumarate and 0.1% of citrate to the cultivation medium of R. erythropolis EK-1 with 2% of n-hexadecane, as well as 0.2% of fumarate to the medium with 2% of ethanol was accompanied by intensification of synthesis of surface-active trehalosemycolates, that is evidenced by the 1.3-1.6-fold increase of the amount of synthesized surface-active substances under such conditions, and by 3-5-fold increase of activity of phosphoenolpyruvate synthetase (key enzyme ofgluconeogenesis) and trehalosephosphate synthase. The paper is presented in Ukrainian.
Pyroh
T P
TP
Shevchuk
T A
TA
Klymenko
Iu O
IuO
D000473:Alkanes / Q000737:chemistry; D002951:Citrates / Q000737:chemistry; D003470:Culture Media; D000431:Ethanol / Q000737:chemistry; D005650:Fumarates / Q000737:chemistry; D005964:Glucosyltransferases / Q000378:metabolism; D006017:Glycolipids / Q000096:biosynthesis*; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000378:metabolism; D017866:Phosphotransferases (Paired Acceptors) / Q000378:metabolism; D012240:Rhodococcus / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013501:Surface-Active Agents
D000473:Alkanes; D002951:Citrates; D003470:Culture Media; D005650:Fumarates; D006017:Glycolipids; D013501:Surface-Active Agents; D000431:Ethanol; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase; D017866:Phosphotransferases (Paired Acceptors); C022509:pyruvate, water dikinase; D019764:Phosphoenolpyruvate Carboxykinase (ATP); C007932:n-hexadecane