Mechanical properties of cellularly responsive hydrogels and their experimental determination.
Hydrogels are increasingly employed as multidimensional cell culture platforms often with a necessity that they respond to or control the cellular environment. Specifically, synthetic hydrogels, such as poly(ethylene glycol) (PEG)-based gels, are frequently utilized for probing the microenvironment's influence on cell function, as the gel properties can be precisely controlled in space and time. Synthetically tunable parameters, such as monomer structure and concentration, facilitate initial gel property control, while incorporation of responsive degradable units enables cell- and/or user-directed degradation. Such responsive gel systems are complex with dynamic changes occurring over multiple time-scales, and cells encapsulated in these synthetic hydrogels often experience and dictate local property changes profoundly different from those in the bulk material. Consequently, advances in bulk and local measurement techniques are needed to monitor property evolution quantatively and understand its effect on cell function. Here, recent progress in cell-responsive PEG hydrogel synthesis and mechanical property characterization is reviewed.
Kloxin
April M
AM
Department of Chemical and Biological Engineering, University of Colorado, Boulder, 80309, USA.
Kloxin
Christopher J
CJ
Bowman
Christopher N
CN
Anseth
Kristi S
KS
D002468:Cell Physiological Phenomena; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D055595:Mechanical Phenomena; D011092:Polyethylene Glycols / Q000737:chemistry
D020100:Hydrogels; D011092:Polyethylene Glycols
Langmuir. 2005 Mar 29;21(7):3084-9
15779988
Science. 1997 May 30;276(5317):1425-8
9162012
Langmuir. 2009 Jan 20;25(2):716-23
19138157
Biomaterials. 2006 Apr;27(10):2265-74
16318872
Biomaterials. 2009 Oct;30(30):6048-54
19674784
Soft Matter. 2009;5:1918-1924
19672325
Biophys J. 2009 Aug 19;97(4):1013-21
19686648
J Orthop Res. 2004 Sep;22(5):1143-9
15304291
Adv Mater. 2009 Dec 28;21(48):5005-5010
25377720
J Biomater Sci Polym Ed. 2004;15(6):701-26
15255521
Nat Mater. 2010 Jan;9(1):82-8
19838182
Biomaterials. 2008 Jul;29(20):2962-8
18433863
Biomaterials. 2008 Jan;29(3):314-26
17953986
J Biomater Sci Polym Ed. 2000;11(5):439-57
10896041
Adv Mater. 2010 Jan 5;22(1):61-6
20217698
J Biomech. 2010 Jan 5;43(1):45-54
19819457
Clin Exp Metastasis. 2009;26(4):289-98
18600304
Nat Biotechnol. 2005 Jan;23(1):47-55
15637621
Phys Rev Lett. 1995 Dec 11;75(24):4425-4428
10059905
Methods Cell Biol. 2007;83:47-65
17613304
J Biol Chem. 2004 Jun 18;279(25):26323-30
15096506
Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22108-13
20018765
Soft Matter. 2009 Jan 1;5(4):740-742
20046915
Science. 2004 Feb 27;303(5662):1352-5
14739465
Tissue Eng. 2003 Aug;9(4):679-88
13678446
Biomaterials. 2007 Jul;28(20):3163-70
17395258
Prog Biophys Mol Biol. 2008 Jun-Jul;97(2-3):163-79
18406455
Acta Biomater. 2007 Sep;3(5):615-29
17572164
Curr Opin Cell Biol. 2009 Dec;21(6):864-70
19740640
Biomaterials. 2009 Sep;30(26):4287-96
19464053
Biomacromolecules. 2003 May-Jun;4(3):713-22
12741789
J Cell Sci. 2003 Jun 15;116(Pt 12):2377-88
12766184
Adv Mater. 2009 Sep 4;21(32-33):3307-29
20882499
Langmuir. 2004 Jun 22;20(13):5153-6
15986641
Faraday Discuss. 2003;123:323-34; discussion 401-21
12638868
Cell Stem Cell. 2009 Jul 2;5(1):17-26
19570510
Biophys J. 2005 Aug;89(2):1374-88
15923238
Science. 2006 Oct 13;314(5797):298-300
17038622
Nat Rev Mol Cell Biol. 2002 Dec;3(12):906-18
12461557
Biomacromolecules. 2008 Mar;9(3):842-9
18288800
Macromolecules. 2009 Jul 28;42(14):5310-5316
21494422
Tissue Eng. 2006 Sep;12(9):2695-706
16995803
Science. 2006 Apr 14;312(5771):217-24
16614209
Nat Cell Biol. 2007 Aug;9(8):893-904
17618273
Biomaterials. 2003 Oct;24(23):4245-52
12853256
Biomaterials. 2008 Jun;29(17):2637-45
18377982
J Am Chem Soc. 2007 Apr 18;129(15):4518-9
17381090
Biomaterials. 2006 Oct;27(28):4881-93
16762407
Proc Natl Acad Sci U S A. 2008 Aug 12;105(32):11305-10
18685096
Biomacromolecules. 2005 Jan-Feb;6(1):290-301
15638532
J Chem Phys. 2009 Oct 7;131(13):134904
19814572
Mol Biol Cell. 2004 Dec;15(12):5231-41
15371537
J Cell Sci. 2008 Oct 15;121(Pt 20):3285-92
18843115
Cell. 2006 Aug 25;126(4):677-89
16923388
Curr Opin Chem Biol. 2008 Feb;12(1):60-5
18282482
Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5413-8
12686696
Phys Rev Lett. 2000 Jul 24;85(4):888-91
10991424
Mol Cancer Ther. 2007 Feb;6(2):552-61
17267658
Science. 2008 Mar 28;319(5871):1812-6
18369143
Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18457-62
19846768
Annu Rev Biophys. 2009;38:301-26
19416071
Biophys J. 2004 Jun;86(6):4004-14
15189896
Biomacromolecules. 2009 Jun 8;10(6):1484-9
19408953
Biophys J. 2008 Dec 15;95(12):6044-51
18775964
Biophys J. 2008 Nov 1;95(9):4426-38
18658232
Biopolymers. 1996;40(4):399-416
8765610
Biophys J. 1999 Feb;76(2):1063-71
9916038
Tissue Eng. 2007 Oct;13(10):2369-85
17658993
Nat Rev Mol Cell Biol. 2002 May;3(5):349-63
11988769
Soft Matter. 2008 Apr 15;4(5):1048-1058
32907138
Biophys J. 2007 Dec 15;93(12):4453-61
18045965
Nat Mater. 2009 Aug;8(8):659-64
19543279
Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13661-5
9391082
Phys Rev Lett. 1995 Feb 13;74(7):1250-1253
10058972
Biophys J. 2002 Dec;83(6):3162-76
12496086
Adv Drug Deliv Rev. 2007 Nov 10;59(13):1306-18
17920155
Biomaterials. 2001 Nov;22(22):3045-51
11575479
Phys Rev Lett. 2003 Oct 10;91(15):158302
14611506
Phys Rev Lett. 2000 Aug 21;85(8):1774-7
10970611
Langmuir. 2009 Oct 6;25(19):11827-34
19788229
Science. 1994 Apr 29;264(5159):696-8
8171320
Proc Natl Acad Sci U S A. 2008 Jun 10;105(23):7947-52
18541916
Curr Opin Biotechnol. 2005 Aug;16(4):422-6
16006115