Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeast--the only other eukaryote for which a comprehensive knockout library exists--revealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.
Kim
Dong-Uk
DU
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Hayles
Jacqueline
J
Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.
Kim
Dongsup
D
Department of Bio and Brain Engineering, Korea Advanced Institute of Science & Technology (KAIST), Yuseong, Daejeon, Korea.
Wood
Valerie
V
Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.|Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1HH, UK.
Park
Han-Oh
HO
Bioneer Corporation, Daedeok, Daejeon, Korea.
Won
Misun
M
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Yoo
Hyang-Sook
HS
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Duhig
Trevor
T
Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.
Nam
Miyoung
M
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Palmer
Georgia
G
Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.
Han
Sangjo
S
Department of Bio and Brain Engineering, Korea Advanced Institute of Science & Technology (KAIST), Yuseong, Daejeon, Korea.
Jeffery
Linda
L
Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.
Baek
Seung-Tae
ST
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Lee
Hyemi
H
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Shim
Young Sam
YS
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Lee
Minho
M
Department of Bio and Brain Engineering, Korea Advanced Institute of Science & Technology (KAIST), Yuseong, Daejeon, Korea.
Kim
Lila
L
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Heo
Kyung-Sun
KS
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Noh
Eun Joo
EJ
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Lee
Ah-Reum
AR
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Jang
Young-Joo
YJ
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Chung
Kyung-Sook
KS
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Choi
Shin-Jung
SJ
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Park
Jo-Young
JY
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Park
Youngwoo
Y
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Kim
Hwan Mook
HM
Bioevaluation Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang, Chungcheongbuk-do, Korea.
Park
Song-Kyu
SK
Bioevaluation Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang, Chungcheongbuk-do, Korea.
Park
Hae-Joon
HJ
Bioneer Corporation, Daedeok, Daejeon, Korea.
Kang
Eun-Jung
EJ
Bioneer Corporation, Daedeok, Daejeon, Korea.
Kim
Hyong Bai
HB
Department of Bioinformatics & Biotechnology, Korea University, Jochiwon, Chungnam, Korea.
Kang
Hyun-Sam
HS
School of Biological Sciences, Seoul National University, Seoul, Korea.
Park
Hee-Moon
HM
Department of Microbiology, Chungnam National University, Yuseong, Daejeon, Korea.
Kim
Kyunghoon
K
Division of Life Sciences, Kangwon National University, Chuncheon, Kangwon-do, Korea.
Song
Kiwon
K
Department of Biochemistry, Yonsei University, Seoul, Korea.
Song
Kyung Bin
KB
Department of Food and Nutrition, Chungnam National University, Yuseong, Daejeon, Korea.
Nurse
Paul
P
Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.|The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA.
Hoe
Kwang-Lae
KL
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.|Bioevaluation Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang, Chungcheongbuk-do, Korea.
D004171:Diploidy; D017353:Gene Deletion; D020043:Genes, Essential / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics; D016681:Genome, Fungal / Q000235:genetics*; D006238:Haploidy; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
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