title: [Features of epidemiology and diagnostics of toxoplasmosis during HIV-infection].
abstract: OBJECTIVE
  Comparative assessment of effectiveness of serologic methods for toxoplasmosis diagnostics in patients with HIV-infection.
  
  
  METHODS
  Sera and CSF samples from 166 patients with AIDS stage IIIB were tested for antibodies to Toxoplasma gondii by indirect immunofluorescence, ELISA and immunoblotting. Results of serological tests were compared with clinical, pathological data as well as with results of MRI and PCR.
  
  
  RESULTS
  Clinical value of IgG detection in blood and CSF by ELISA was shown--high level of antibodies marked reactivation of the invasion. IgG antibodies in CSF were detected only if high levels of IgG were present in the blood. Detection of antigenic determinants with molecular mass 18 - 20 and 65 - 70 kDa in immunoblotting was proposed as a criterion of cerebral toxoplasmosis reactivation.
  
  
  CONCLUSIONS
  Complex laboratory serologic tests along with data obtained by MRI, PCR and microscopy of T. gondii cysts enhances the effectiveness of toxoplasmosis diagnostics. Knowledge of toxoplasmosis reactivation criteria in patients with AIDS will allow to develop the optimal protocol of toxoplasmosis diagnostics and substantiate measures for its prevention.
authors.forename: E V
authors.initials: EV
authors.lastname: Gubareva
authors.forename: D B
authors.initials: DB
authors.lastname: Goncharov
authors.forename: A B
authors.initials: AB
authors.lastname: Peregudova
authors.forename: Iu G
authors.initials: IuG
authors.lastname: Parkhomenko
authors.forename: I M
authors.initials: IM
authors.lastname: Tishkevich
authors.forename: O A
authors.initials: OA
authors.lastname: Tishkevich
authors.forename: N V
authors.initials: NV
authors.lastname: Kobets
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology*; D000328:Adult; D000368:Aged; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D000953:Antigens, Protozoan / Q000032:analysis / Q000276:immunology; D015153:Blotting, Western / Q000379:methods; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D019084:Fluorescent Antibody Technique, Indirect; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D008875:Middle Aged; D012426:Russia / Q000453:epidemiology; D014122:Toxoplasma / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology*; D016781:Toxoplasmosis, Cerebral / Q000175:diagnosis
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000939:Epitopes; D007074:Immunoglobulin G