title: The fusogen EFF-1 controls sculpting of mechanosensory dendrites.
abstract: The mechanisms controlling the formation and maintenance of neuronal trees are poorly understood. We examined the dynamic development of two arborized mechanoreceptor neurons (PVDs) required for reception of strong mechanical stimuli in Caenorhabditis elegans. The PVDs elaborated dendritic trees comprising structural units we call "menorahs." We studied how the number, structure, and function of menorahs were maintained. EFF-1, an essential protein mediating cell fusion, acted autonomously in the PVDs to trim developing menorahs. eff-1 mutants displayed hyperbranched, disorganized menorahs. Overexpression of EFF-1 in the PVD reduced branching. Neuronal pruning appeared to involve EFF-1-dependent branch retraction and neurite-neurite autofusion. Thus, EFF-1 activities may act as a quality control mechanism during the sculpting of dendritic trees.
authors.affiliation: Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel.
authors.forename: Meital
authors.initials: M
authors.lastname: Oren-Suissa
authors.forename: David H
authors.initials: DH
authors.lastname: Hall
authors.forename: Millet
authors.initials: M
authors.lastname: Treinin
authors.forename: Gidi
authors.initials: G
authors.lastname: Shemer
authors.forename: Benjamin
authors.initials: B
authors.lastname: Podbilewicz
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D021621:Imaging, Three-Dimensional; D008465:Mechanoreceptors / Q000378:metabolism* / Q000648:ultrastructure*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D008959:Models, Neurological; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure*; D013696:Temperature
chemical_list: D029742:Caenorhabditis elegans Proteins; C470734:EFF-1 protein, C elegans; D008562:Membrane Glycoproteins; D050505:Mutant Proteins
references.citation: Nat Neurosci. 2001 Apr;4(4):359-65
references.pmid: 11276225
references.citation: Curr Opin Neurobiol. 2004 Feb;14(1):74-82
references.pmid: 15018941
references.citation: Dev Biol. 2003 Nov 1;263(1):81-102
references.pmid: 14568548
references.citation: Genes Dev. 1989 Dec;3(12A):1823-33
references.pmid: 2576011
references.citation: Nature. 2009 Aug 20;460(7258):978-83
references.pmid: 19633650
references.citation: Annu Rev Biochem. 2003;72:175-207
references.pmid: 14527322
references.citation: J Neurosci. 2010 Mar 3;30(9):3175-83
references.pmid: 20203177
references.citation: Philos Trans R Soc Lond B Biol Sci. 1986 Nov 12;314(1165):1-340
references.pmid: 22462104
references.citation: Brain Res. 1999 Nov 6;846(2):243-52
references.pmid: 10556642
references.citation: Nat Rev Mol Cell Biol. 2008 Jul;9(7):543-56
references.pmid: 18496517
references.citation: Cell. 2008 Dec 26;135(7):1276-86
references.pmid: 19084269
references.citation: Cell. 2009 Aug 7;138(3):549-61
references.pmid: 19665976
references.citation: J Neurobiol. 2004 Oct;61(1):161-74
references.pmid: 15362159
references.citation: Dev Cell. 2006 Oct;11(4):471-81
references.pmid: 17011487
references.citation: EMBO J. 2002 Mar 1;21(5):1012-20
references.pmid: 11867529
references.citation: Dev Cell. 2002 Mar;2(3):355-62
references.pmid: 11879640
references.citation: Dev Cell. 2007 May;12(5):683-98
references.pmid: 17488621