Demineralization Depth Using QLF and a Novel Image Processing Software.
Quantitative Light-Induced fluorescence (QLF) has been widely used to detect tooth demineralization indicated by fluorescence loss with respect to surrounding sound enamel. The correlation between fluorescence loss and demineralization depth is not fully understood. The purpose of this project was to study this correlation to estimate demineralization depth. Extracted teeth were collected. Artificial caries-like lesions were created and imaged with QLF. Novel image processing software was developed to measure the largest percent of fluorescence loss in the region of interest. All teeth were then sectioned and imaged by polarized light microscopy. The largest depth of demineralization was measured by NIH ImageJ software. The statistical linear regression method was applied to analyze these data. The linear regression model was Y = 0.32X + 0.17, where X was the percent loss of fluorescence and Y was the depth of demineralization. The correlation coefficient was 0.9696. The two-tailed t-test for coefficient was 7.93, indicating the P-value = .0014. The F test for the entire model was 62.86, which shows the P-value = .0013. The results indicated statistically significant linear correlation between the percent loss of fluorescence and depth of the enamel demineralization.
Wu
Jun
J
Dental Branch, University of Texas Health Science Center at Houston, 6516 John Freeman Boulevard, Houston, TX 77030-3402, USA.
Donly
Zachary R
ZR
Donly
Kevin J
KJ
Hackmyer
Steven
S
Dent Clin North Am. 2000 Oct;44(4):955-69, ix
11048282
Eur J Orthod. 2007 Jun;29(3):294-8
17483494
Caries Res. 1987;21(4):368-74
3475183
Caries Res. 1997;31(2):103-10
9118181
J Dent Res. 1984 Nov;63(11):1271-5
6094633
Oper Dent. 2009 Mar-Apr;34(2):136-41
19363968
Adv Dent Res. 1997 Nov;11(4):502-6
9470510
Dentomaxillofac Radiol. 2001 Nov;30(6):298-307
11641727
J Dent Educ. 1993 Jun;57(6):439-43
8315101
Pediatr Dent. 1999 Mar-Apr;21(2):109-13
10197335
J Dent. 2008 Dec;36(12):1033-40
18930575
J Appl Oral Sci. 2008 Jan-Feb;16(1):18-23
19089284
Eur J Orthod. 2003 Jun;25(3):217-23
12831210
Spec Care Dentist. 2003;23(1):18-21
12887149
J Dent Educ. 1998 Oct;62(10):771-80
9847881
Caries Res. 2003 Jan-Feb;37(1):8-16
12566633
Caries Res. 1990;24(6):381-96
2289223
J Dent. 2009 May;37(5):336-41
19237234
Int Dent J. 2008 Aug;58(4):208-12
18783113
Community Dent Oral Epidemiol. 2005 Aug;33(4):265-73
16008633
Community Dent Oral Epidemiol. 1987 Apr;15(2):90-4
3552397
Caries Res. 2007;41(5):358-64
17713335
Eur J Oral Sci. 1996 Aug;104(4 ( Pt 2)):470-6
8930599
Caries Res. 1993;27(4):307-11
8402807
Caries Res. 1996;30(6):381-8
8946093
Caries Res. 1997;31(2):125-31
9118184
J Am Dent Assoc. 2005 Dec;136(12):1682-7
16383050
J Am Dent Assoc. 2008 Oct;139(10):1374-81
18832273
Pediatrics. 2000 Dec;106(6):E84
11099627
Caries Res. 1999 May-Jun;33(3):220-6
10207198
J Dent. 2009 May;37(5):397-405
19250731
J Biol Buccale. 1988 Mar;16(1):13-7
3165088
Dent Mater J. 2009 Sep;28(5):523-9
19822981
Caries Res. 1991;25(4):296-303
1913769
J Dent Res. 1997 Apr;76(4):875-82
9126184
ASDC J Dent Child. 1988 Sep-Oct;55(5):343-7
3170872
Caries Res. 2005 May-Jun;39(3):173-7
15914977
Community Dent Oral Epidemiol. 1992 Jun;20(3):113-7
1623699
Pediatr Dent. 2006 Sep-Oct;28(5):431-7
17036709
Am J Dent. 2006 Feb;19(1):15-8
16555651
Caries Res. 1982;16(3):201-10
6953998
Caries Res. 1992;26(6):445-9
1294305
Dent Mater. 2003 Jul;19(5):368-74
12742431
Caries Res. 2009;43(6):442-8
19907175
J Public Health Dent. 1995 Summer;55(3):181-8
7562733
MMWR Surveill Summ. 2005 Aug 26;54(3):1-43
16121123
Dent Mater. 2009 Jun;25(6):703-8
19150126
Br Dent J. 1983 Apr 9;154(7):201-6
6573898