Cytogenetic map of common bean (Phaseolus vulgaris L.).
A cytogenetic map of common bean was built by in situ hybridization of 35 bacterial artificial chromosomes (BACs) selected with markers mapping to eight linkage groups, plus two plasmids for 5S and 45S ribosomal DNA and one bacteriophage. Together with three previously mapped chromosomes (chromosomes 3, 4, and 7), 43 anchoring points between the genetic map and the cytogenetic map of the species are now available. Furthermore, a subset of four BAC clones was proposed to identify the 11 chromosome pairs of the standard cultivar BAT93. Three of these BACs labelled more than a single chromosome pair, indicating the presence of repetitive DNA in their inserts. A repetitive distribution pattern was observed for most of the BACs; for 38% of them, highly repetitive pericentromeric or subtelomeric signals were observed. These distribution patterns corresponded to pericentromeric and subtelomeric heterochromatin blocks observed with other staining methods. Altogether, the results indicate that around half of the common bean genome is heterochromatic and that genes and repetitive sequences are intermingled in the euchromatin and heterochromatin of the species.
Fonsêca
Artur
A
Laboratory of Plant Cytogenetics, Department of Botany, Federal University of Pernambuco, Recife, PE, 50670-420, Brazil.
Ferreira
Joana
J
dos Santos
Tiago Ribeiro Barros
TR
Mosiolek
Magdalena
M
Bellucci
Elisa
E
Kami
James
J
Gepts
Paul
P
Geffroy
Valérie
V
Schweizer
Dieter
D
dos Santos
Karla G B
KG
Pedrosa-Harand
Andrea
A
D002874:Chromosome Mapping / Q000379:methods*; D020732:Cytogenetic Analysis; D022041:Euchromatin / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics*; D006570:Heterochromatin / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
D022041:Euchromatin; D006570:Heterochromatin
Trends Genet. 2007 Mar;23(3):134-9
17275131
Genetics. 2008 Aug;179(4):2299-312
18689902
Chromosome Res. 2007;15(1):77-84
17295128
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W516-20
15980525
Trends Genet. 1992 Nov;8(11):372-3
1440872
Genetics. 2002 Aug;161(4):1661-72
12196409
Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W670-5
16845095
Theor Appl Genet. 1993 Jan;85(5):513-20
24195923
Plant J. 2001 Jul;27(1):49-58
11489182
Genetics. 2009 Feb;181(2):405-19
19087965
BMC Genomics. 2006 Aug 14;7:207
16907970
Mol Plant Microbe Interact. 2000 Mar;13(3):287-96
10707354
Methods Mol Biol. 2000;132:365-86
10547847
Plant Mol Biol. 1999 Aug;40(6):977-83
10527422
Theor Appl Genet. 2003 Jan;106(2):205-12
12582845
Genetics. 2005 Feb;169(2):955-65
15489513
Genetics. 2005 Dec;171(4):1963-76
16143604
Chromosome Res. 2008;16(7):919-33
18688733
Genetics. 2002 May;161(1):345-53
12019248
Theor Appl Genet. 2004 Aug;109(4):690-9
15221144
Plant Physiol. 2005 Apr;137(4):1228-35
15824285
Chromosoma. 1996 Oct;105(4):231-6
8854882
Genome. 1994 Dec;37(6):915-24
7828839
Theor Appl Genet. 2006 Apr;112(6):987-98
16404584
Cytogenet Genome Res. 2008;120(3-4):194-201
18504347
Curr Opin Plant Biol. 2006 Apr;9(2):104-9
16458041
Theor Appl Genet. 1993 Oct;87(1-2):38-43
24190189
Plant Physiol. 2005 Apr;137(4):1174-81
15824279
Plant Physiol. 2009 Nov;151(3):1048-65
19776165
Chromosome Res. 2009;17(3):405-17
19330455
Plant J. 1997 May;11(5):1007-16
9193072
Plant J. 2003 Mar;33(6):967-73
12631322
Genome. 1997 Feb;40(1):138-42
18464813
Biotech Histochem. 2010 Apr;85(2):115-25
19657781
Genome. 2006 Sep;49(9):1057-68
17110986
Theor Appl Genet. 2006 Mar;112(5):924-33
16397788
Theor Appl Genet. 2003 Jan;106(2):251-61
12582850
Genome. 1999 Dec;42(6):1224-33
10659791
Nat Genet. 2004 Feb;36(2):138-45
14716315
Genetics. 2001 Apr;157(4):1749-57
11290728
Genetics. 1992 Jul;131(3):733-40
1352759
Plant J. 2009 Jun;58(5):857-69
19207213
Chromosoma. 2004 Dec;113(6):276-83
15480726
Theor Appl Genet. 2010 Jun;121(1):87-103
20182695