[Change of gonad gene expression profile in male F344 rats after exposure to 1-bromopropane].
OBJECTIVE
To study the 1-bromopropane (1-BP)-induced altered gene expression profiles in rat gonad, and explore its male reproductive toxicity-related mRNA changes.


METHODS
Twelve F344/NSIc male rats were randomly divided into two groups of 6 each. Rats were exposed to either fresh air or 5030 mg/m3 1-BP through inhalation for 8 h. Rats were sacrificed and testes were removed at 16 h after exposure. Global changes in gene expression were determined by microarray analysis using rat genital chip followed by Real-time PCR validation.


RESULTS
Among the 5082 genes and ESTs in the genital chip, 62 genes were down-regulated and 3 genes were up-regulated by 1-BP, which include synthetic sex hormone-related genes cytochrome P450 aromatase (CYP19a), glutathione S-transferase (GSTT1), creatine kinase (Ckb), myelin and lymphocyte protein (Mal) and S100 calcium-binding protein (S100a4). Classification analysis revealed that the majority of gene changes was involved protein/lipid metabolism, followed by the stress-associated defense response. Real-time PCR confirmed the down-regulation of CYP19a, GSTT1, Mal and S100a4 genes.


CONCLUSIONS
Acute high-dose exposure to 1-BP causes the down-regulation of testicular CYP19a, S100a4, GSTT1 and Mal. This altered gene profiles might reflect the toxic mechanism which suggested that 1-BP disrupt the metabolics and endocrine balance.
Li
Weihua
W
School of Public Health, MO Key Lab. for Public Health Safety, Fudan University, Shanghai 200032, China.
Ichihara
Gaku
G
Wang
Hailan
H
Kitakawa
Emiko
E
Iwahashi
Hitoshi
H
Ding
Xuncheng
X
Zhou
Zhijun
Z
D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D004781:Environmental Exposure / Q000009:adverse effects*; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006842:Hydrocarbons, Brominated / Q000633:toxicity; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D060888:Real-Time Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism*
D006842:Hydrocarbons, Brominated; D012333:RNA, Messenger; D001141:Aromatase; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase; C118559:1-bromopropane