The NucleoSpin® DNA Clean-up XS kit for the concentration and purification of genomic DNA extracts: an alternative to microdialysis filtration.
Traditionally, DNA extracts from biological evidence items have been concentrated and rinsed using microdialysis filtration units, including the Centricon(®) and Microcon(®) centrifugal filter devices. As an alternative to microdialysis filtration, we present an optimized method for using NucleoSpin(®) XS silica columns to concentrate and clean-up aqueous extracts from the organic extraction of DNA from biological samples. The method can be used with standard organic extraction and dithiothreitol (DTT)-based differential extraction methods with no modifications to these methods prior to the concentration and clean-up step. Extracts from laboratory-prepared bloodstains, saliva and semen stains have been successfully amplified with both qPCR and STR assays. Finally, the total time to process a set of samples with the NucleoSpin(®) XS column is approximately 30 min vs. approximately 1.5h with the Centricon(®) YM-100 filter device.
Hudlow
William R
WR
California Department of Justice Jan Bashinski DNA Laboratory, 1001 W. Cutting Blvd., Suite 110, Richmond, CA 94804, USA. Bill.Hudlow@doj.ca.gov
Krieger
Robert
R
Meusel
Markus
M
Sehhat
Joshua C
JC
Timken
Mark D
MD
Buoncristiani
Martin R
MR
D004247:DNA / Q000302:isolation & purification*; D005374:Filtration / Q000379:methods*; D006801:Humans; D017551:Microdialysis / Q000379:methods*; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction
D004247:DNA