title: Fluorescent reporters for studies of cellular localization of proteins in Staphylococcus aureus.
abstract: We have constructed a set of plasmids that allow expression, from their native chromosomal loci, of Staphylococcus aureus proteins fused to one of four different fluorescent proteins (green fluorescent protein [GFP], cyan fluorescent protein [CFP], yellow fluorescent protein [YFP], and mCherry), using two different resistance markers (kanamycin and erythromycin). We have also constructed a plasmid that allows expression of proteins from the ectopic spa locus in the S. aureus chromosome. This toolbox can be used for studies of the localization of proteins in S. aureus, a prominent pathogen in both health care and community settings.
authors.affiliation: Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República, Apartado 127, 2781-901 Oeiras, Portugal.
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Pereira
authors.forename: Helena
authors.initials: H
authors.lastname: Veiga
authors.forename: Ana M
authors.initials: AM
authors.lastname: Jorge
authors.forename: Mariana G
authors.initials: MG
authors.lastname: Pinho
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D013211:Staphylococcus aureus / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins
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