title: [Comparison of two nonalcoholic hepatocellular steatosis models].
abstract: OBJECTIVE
  To compare two models of nonalcoholic hepatocellular steatosis.
  
  
  METHODS
  HL-7702 cells were incubated with a mixture of of unsaturated oleate acid or 50% fetal bovine serum to induce fat-overloading. Significant fat accumulation was documented by Oil Red O staining , and intracellular triglyceride levels was detected by triglyceride enzymatic assay.
  
  
  RESULTS
  The results showed that both 0.5 mmol/ml oleate acid and 50% FBS were able to induce nonalcoholic hepatocellular steatosis.
  
  
  CONCLUSIONS
  A nonalcoholic hepatocellular steatosis was induced by 0.5 mmol/ml oleate acid.
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Hong-ru
authors.initials: HR
authors.lastname: Wu
authors.forename: Shao-hua
authors.initials: SH
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: You-ming
authors.initials: YM
authors.lastname: Li
mesh_terms: D002460:Cell Line; D005234:Fatty Liver; D022781:Hepatocytes / Q000473:pathology*; D006801:Humans; D008954:Models, Biological