Sow vaccination modulates the colonization of piglets by Haemophilus parasuis.
Haemophilus parasuis is the etiologic agent of Glässer's disease in pigs and a colonizer of the upper respiratory tract of healthy pigs. A good balance between colonization and immunity is important to avoid a disease outbreak. This work studied the colonization of H. parasuis in healthy piglets coming from vaccinated and non-vaccinated sows. Piglets from vaccinated sows had higher IgG levels at early time points and subsequently were colonized later and to a lower degree than piglets from non-vaccinated ones. The variability of H. parasuis isolates was investigated by 2 genotyping methods: enterobacterial repetitive intergenic consensus (ERIC)-PCR and multilocus sequence typing (MLST). A high turnover of strains was found in both groups of piglets, with few strains found on more than one sampling occasion. We found a higher number of H. parasuis strains (16 strains) within a given farm than previously thought. Overall, more H. parasuis diversity was found in piglets from non-vaccinated sows than in those from vaccinated sows. These results indicate that vaccination of sows in a farm delays the colonization of piglets and reduces the carriage and heterogeneity of H. parasuis strains.
Cerdà-Cuéllar
M
M
Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus Univ. Autònoma de Barcelona, Bellaterra, Barcelona, Spain. marta.cerda@cresa.uab.cat
Naranjo
J F
JF
Verge
A
A
Nofrarías
M
M
Cortey
M
M
Olvera
A
A
Segalés
J
J
Aragon
V
V
D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology* / Q000494:pharmacology; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods / Q000662:veterinary; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006192:Haemophilus Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000235:genetics / Q000276:immunology*; D007112:Immunity, Maternally-Acquired / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012140:Respiratory Tract Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D014611:Vaccination / Q000379:methods / Q000662:veterinary
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines