Novel carbohydrate-binding module identified in a ruminal metagenomic endoglucanase.
Endoglucanase C5614-1 comprises a catalytic module (CM) and an X module (XM). The XM showed no significant homology with known carbohydrate-binding modules (CBMs). Recombinant full-length endoglucanase could bind Avicel, whereas the CM could not. The XM could bind various polysaccharides. The results demonstrated that the XM was a new CBM.
Duan
Cheng-Jie
CJ
College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530004, People's Republic of China.
Liu
Jun-Liang
JL
Wu
Xi
X
Tang
Ji-Liang
JL
Feng
Jia-Xun
JX
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002020:Buffaloes; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism*; D054892:Metagenome; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding; D012417:Rumen / Q000382:microbiology*; D016415:Sequence Alignment
D011134:Polysaccharides; D002480:Cellulase
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