title: Molecular characterization and differential expression of beta-1,3-glucanase during ripening in banana fruit in response to ethylene, auxin, ABA, wounding, cold and light-dark cycles.
abstract: beta-1,3-Glucanases (E.C. 3.2.1.39) are widely distributed enzyme among bacteria, fungi, and higher plants. Analyses of accumulation levels of beta-1,3-glucanase protein in various tissues in banana have clearly indicated abundance of beta-1,3-glucanase protein accumulation in ripe pulp tissue. After cloning of beta-1,3-glucanase from banana pulp (cultivar Cavendish), we have carried out an in silico analysis to investigate the sequential, structural, and phylogenetic characteristics of the putative banana beta-1,3-glucanase protein. As like other ripening specific genes, beta-1,3-glucanase is regulated in response to a wide variety of factors. Therefore, we have analyzed the transcript accumulation pattern and protein levels of beta-1,3-glucanase in response to ethylene, auxin, ABA, wounding and, low temperature in preclimacteric banana fruit. Expression profile analyses have indicated that whereas exogenous application of ethylene strongly stimulated beta-1,3-glucanase transcript accumulation, ABA partially induced the expression of the gene. On the other hand, wound treatment did not induce beta-1,3-glucanase expression. Conversely, auxin and cold treatment negatively regulated beta-1,3-glucanase gene expression and thus inhibited glucanase activity. In addition, beta-1,3-glucanase transcript level was markedly decreased by constant exposure to white light. Protein level and enzymatic activity of beta-1,3-glucanase were substantially increased with considerable decrease in fruit firmness by ethylene treatment and reduced exposure to white light conditions as compared with other treatments. Together, the overall study of beta-1,3-glucanase expression pattern, glucanase activity, and changes in fruit firmness during ripening in various conditions suggest the possible physiological function of beta-1,3-glucanase in fruit pulp softening.
authors.affiliation: Division of Plant Biology, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Kolkata, West Bengal, 700 009, India. src.bose@gmail.com
authors.forename: Swarup
authors.initials: S
authors.lastname: Roy Choudhury
authors.forename: Sujit
authors.initials: S
authors.lastname: Roy
authors.forename: Sanjay Kumar
authors.initials: SK
authors.lastname: Singh
authors.forename: Dibyendu N
authors.initials: DN
authors.lastname: Sengupta
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D005030:Ethylenes / Q000494:pharmacology; D005638:Fruit / Q000201:enzymology*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D043326:Glucan 1,3-beta-Glucosidase / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000494:pharmacology; D008969:Molecular Sequence Data; D028521:Musa / Q000201:enzymology*; D017440:Photoperiod; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism; D017434:Protein Structure, Tertiary; D018749:RNA, Plant / Q000235:genetics; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological
chemical_list: D005030:Ethylenes; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D018749:RNA, Plant; D000040:Abscisic Acid; C036216:ethylene; D043326:Glucan 1,3-beta-Glucosidase
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