Competitive adsorption of thiolated polyethylene glycol and mercaptopropionic acid on gold nanoparticles measured by physical characterization methods.
Competitive adsorption kinetics between thiolated polyethylene glycol (SH-PEG) and mercaptopropionic acid (MPA) on gold nanoparticles (Au-NPs) were studied using a prototype physical characterization approach that combines dynamic light scattering (DLS) and electrospray differential mobility analysis (ES-DMA). The change in hydrodynamic particle size (intensity average) due to the formation of SH-PEG coatings on Au-NPs was measured by DLS in both two-component (Au-NP + MPA or Au-NP + SH-PEG) and three-component (Au-NP +MPA + SH-PEG) systems. ES-DMA was employed to quantify the surface coverage of SH-PEG and establish a correlation between surface coverage and the change in particle size measured by DLS. A change in the equilibrium binding constant for SH-PEG on Au-NPs at various concentrations of SH-PEG and MPA showed that the presence of MPA reduced the binding affinity of SH-PEG to the Au-NP surface. Kinetic studies showed that SH-PEG was desorbed from the Au-NP surface following a second-order desorption model after subsequently introducing MPA. The desorption rate constant of SH-PEG from the Au-NP surface by MPA displacement was strongly affected by the concentration of MPA and the excess SH-PEG in solution.
Tsai
De-Hao
DH
Ceramics Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA.
DelRio
Frank W
FW
MacCuspie
Robert I
RI
Cho
Tae Joon
TJ
Zachariah
Michael R
MR
Hackley
Vincent A
VA
D000327:Adsorption; D006046:Gold; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D008722:Methods; D011092:Polyethylene Glycols / Q000737:chemistry*; D011422:Propionates / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry
D011422:Propionates; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols; D006046:Gold
Biotechnol Bioeng. 2009 Feb 15;102(3):845-55
18958863
Mol Cancer Ther. 2006 Apr;5(4):1014-20
16648573
J Biol Chem. 1987 May 25;262(15):6951-4
3034874
J Am Chem Soc. 2005 Feb 23;127(7):2172-83
15713095
Biochemistry. 1974 Feb 26;13(5):952-60
4591621
Small. 2009 May;5(9):1030-4
19291731
J Colloid Interface Sci. 2005 Feb 1;282(1):46-57
15576080
Nanomedicine. 2009 Jun;5(2):106-17
19071065
Mol Cancer Ther. 2007 Jul;6(7):2039-47
17620433
Langmuir. 2009 Jan 6;25(1):140-6
19063636
J Biol Chem. 1986 Nov 15;261(32):14871-4
3021754
Int J Hyperthermia. 2007 Sep;23(6):501-11
17952764
Anal Chem. 2000 Nov 15;72(22):5535-41
11101228
Langmuir. 2008 Aug 19;24(16):8483-90
18661963
ACS Nano. 2008 Nov 25;2(11):2263-72
19206392
J Biol Chem. 1989 Sep 5;264(25):14646-52
2549028
Drug Deliv. 2004 May-Jun;11(3):169-83
15204636
Int J Cancer. 2007 Jun 1;120(11):2474-80
17330231
Phys Rev E Stat Nonlin Soft Matter Phys. 2004 Nov;70(5 Pt 1):051406
15600617
J Phys Chem B. 2009 Aug 6;113(31):11016-22
19603779