The endolysins of bacteriophages CMP1 and CN77 are specific for the lysis of Clavibacter michiganensis strains.
Putative endolysin genes of bacteriophages CMP1 and CN77, which infect Clavibacter michiganensis subsp. michiganensis and C. michiganensis subsp. nebraskensis, respectively, were cloned and expressed in Escherichia coli. The His-tagged endolysin of CMP1 consists of 306 amino acids and has a calculated molecular mass of 34.8 kDa, while the His-tagged endolysin of CN77 has 290 amino acids with a molecular mass of 31.9 kDa. The proteins were purified and their bacteriolytic activity was demonstrated. The bacteriolytic activity of both enzymes showed a host range which was limited to the respective C. michiganensis subspecies and did not affect other bacteria, even those closely related to Clavibacter. Due to the high specificity of the CMP1 and CN77 endolysins they may be useful tools for biocontrol of plant-pathogenic C. michiganensis without affecting other bacteria in the soil.
Wittmann
Johannes
J
Department of Microbiology/Genetechnology, Faculty of Biology, University of Bielefeld, Universitaetsstr. 25, 33615 Bielefeld, Germany.
Eichenlaub
Rudolf
R
Department of Microbiology/Genetechnology, Faculty of Biology, University of Bielefeld, Universitaetsstr. 25, 33615 Bielefeld, Germany.
Dreiseikelmann
Brigitte
B
Department of Microbiology/Genetechnology, Faculty of Biology, University of Bielefeld, Universitaetsstr. 25, 33615 Bielefeld, Germany.
D000192:Actinomycetales / Q000821:virology*; D001433:Bacteriolysis; D001435:Bacteriophages / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D019295:Computational Biology; D004279:DNA, Viral / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D020539:Sequence Analysis, Protein; D013045:Species Specificity
D004279:DNA, Viral; D010450:Endopeptidases; C001143:endolysin