DNA types of aspermic Fasciola species in Japan.
In order to reveal DNA types of aspermic Fasciola forms in Japan, Fasciola specimens obtained from eight prefectures that had not been previously reported were analyzed for DNA of ribosomal internal transcribed spacer 1 (ITS1) and mitochondrial NADH dehydrogenase 1 (ND1) gene. Five combinations in DNA types of both ITS1 and ND1 were revealed from the results of this study and previous studies. The DNA type Fsp2, which is identical to that of F. gigantica in both ITS1 and ND1, was the most predominant in Japan, followed by Fsp1, which is the same DNA type as that of F. hepatica. Fasciola forms with Fsp1 mainly occurred in the northern region of Japan and those with Fsp2 were mainly in the western region. The founder effect related to migration of definitive host and susceptibility of intermediate host snail might play an important role in both geographical distribution and frequency of DNA types in Japanese Fasciola specimens.
Ichikawa
Madoka
M
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University.
Iwata
Noriyuki
N
Itagaki
Tadashi
T
D000818:Animals; D002417:Cattle; D016172:DNA Fingerprinting; D018509:DNA, Helminth / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics; D005209:Fasciola / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D005211:Fascioliasis / Q000662:veterinary*; D005843:Geography; D007564:Japan; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012669:Seminal Vesicles / Q000469:parasitology; D013094:Spermatozoa / Q000469:parasitology
D018509:DNA, Helminth; D021901:DNA, Intergenic