title: Immobilization of cross-linked lipase aggregates onto magnetic beads for enzymatic degradation of polycaprolactone.
abstract: Candida rugosa lipase was immobilized on amino-functionalized magnetic supports via cross-linked enzyme aggregates (CLEA) and used to enhance the enzymatic degradation of polycaprolactone (PCL). The maximum amounts of lipase immobilized on the magnetic beads using glutaraldehyde as a coupling agent were determined to be 33.7 mg/g of beads with an 81% recovery of activity after immobilization. Compared to the free enzyme, the immobilized lipase showed the optimum pH at 1 unit higher (pH 8.0) and also retained its enzymatic activity at higher temperatures. There was 62.9% retention of lipase activity after 30 consecutive reuses, indicating its stability and reusability in aqueous media. Moreover, the immobilized lipase maintained more than 80% of its initial activity during 30 days storage period, while the free lipase lost all under same condition. In addition, the immobilized lipase showed a more than 6-fold increase in biodegradability over the free lipase when the immobilized lipase was used to degrade PCL in a batch system. Higher thermal and storage stability, as well as good durability after repeated use of the immobilized lipase CLEA, highlights its potential applicability as large scale continuous systems for the enzymatic degradation of PCL.
authors.affiliation: Department of Microbiology, Chungbuk National University Sungbong-Ro, Heungduk-Gu, Cheongju, South Korea.
authors.forename: Mina
authors.initials: M
authors.lastname: Kim
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Park
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Um
authors.forename: Dong-Hun
authors.initials: DH
authors.lastname: Lee
authors.forename: Kyu-Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Fumihisa
authors.initials: F
authors.lastname: Kobayashi
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Iwasaka
authors.forename: Chun-Sang
authors.initials: CS
authors.lastname: Hong
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
mesh_terms: D001711:Biotransformation; D002175:Candida / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005976:Glutaral / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008280:Magnetics; D008863:Microspheres; D011091:Polyesters / Q000378:metabolism*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D011091:Polyesters; C016240:polycaprolactone; D008049:Lipase; D005976:Glutaral