Entrapment of proteins in glycogen-capped and hydrazide-activated supports.
A method is described for the entrapment of proteins in hydrazide-activated supports using oxidized glycogen as a capping agent. This approach is demonstrated using human serum albumin (HSA) as a model binding agent. After optimization of this method, a protein content of 43 (+/-1)mg of HSA/g support was obtained for porous silica. The entrapped HSA supports could retain a low-mass drug (S-warfarin) and had activities and equilibrium constants comparable to those for soluble HSA. It was also found that this approach could be used with other proteins and binding agents that had masses between 5.8 and 150kDa.
Jackson
Abby J
AJ
Department of Chemistry, University of Nebraska, Lincoln, 68588, USA.
Xuan
Hai
H
Hage
David S
DS
D006003:Glycogen / Q000737:chemistry*; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D016062:Porosity; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D014859:Warfarin / Q000737:chemistry
D006834:Hydrazines; D012709:Serum Albumin; C029424:hydrazine; D014859:Warfarin; D012822:Silicon Dioxide; D006003:Glycogen
J Chromatogr A. 1994 May 27;669(1-2):9-19
8055106
Biotechniques. 1994 May;16(5):894-7
8068345
Methods Mol Biol. 2000;147:1-6
10857080
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Feb 25;768(1):3-30
11939555
Anal Chem. 1994 Nov 1;66(21):3814-22
7802261
Anal Chem. 1985 Sep;57(11):1099A-1101A, 1102A-1106A passim
4051185
Anal Biochem. 2005 Nov 15;346(2):300-10
16225836
Anal Chem. 2007 Feb 15;79(4):1411-24
17297940
Trends Biotechnol. 2008 Oct;26(10):566-72
18757108