Depletion of CD147 sensitizes human malignant melanoma cells to hydrogen peroxide-induced oxidative stress.
BACKGROUND
Increased sensitivity to reactive oxygen species (ROS) contributes to the effectiveness of therapeutic strategies in patients with malignant melanoma (MM). CD147, a cell surface receptor for cyclophilin A (CypA), is thought to exert antioxidant activities.


OBJECTIVE
To understand the influences and mechanisms of CD147 on proliferation, apoptosis and redox state of A375 cells under H(2)O(2)-induced oxidative stress.


METHODS
The effect of CD147 silencing on cell viability, apoptosis, the generation of ROS, superoxide dismutase (SOD) activity, and the malondialdehyde (MDA) level that reflects oxidative damage, was measured in human malignant melanoma cell line A375 treated or untreated with hydrogen peroxide (H(2)O(2)).


RESULTS
In A375 cells, CD147 silencing increased the H(2)O(2)-induced inhibition of cell viability, H(2)O(2)-induced apoptosis, H(2)O(2)-mediated ROS- and MDA generation, and the H(2)O(2)-triggered decrease in SOD activity.


CONCLUSIONS
Our results demonstrated that CD147 silencing increased cellular ROS and destroyed the intrinsic antioxidant defenses in A375, indicating that CD147 exerts a cytoprotective effect against H(2)O(2)-induced oxidative damage.
Li
Ji
J
Department of Dermatology, XiangYa Hospital, Central South University, Changsha, Hunan, China.
Peng
Lefang
L
Wu
Lisha
L
Kuang
Yehong
Y
Su
Juan
J
Yi
Mei
M
Hu
Xinglin
X
Li
Dai
D
Xie
Hongfu
H
Kanekura
Takuro
T
Chen
Xiang
X
D017209:Apoptosis / Q000502:physiology; D051926:Basigin / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D008315:Malondialdehyde / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D016877:Oxidants / Q000378:metabolism / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species / Q000378:metabolism; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000378:metabolism
C497886:BSG protein, human; D016877:Oxidants; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D051926:Basigin; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase