title: In vitro lung slices: a powerful approach for assessment of lung pathophysiology.
abstract: This review summarizes the existing literature on the use of in vitro lung slices to study pulmonary physiology, pharmacology, pathogenesis and toxicity. Since in vitro lung slices maintain cell-cell and cell-matrix relationships in a highly controllable and accessible setting, they offer many advantages over both in vivo and single-cell culture systems. With the advent of high-production slicers, lung slices can be rapidly and reproducibly generated, including from animals treated in vivo. Slices can then be treated in vitro and analyzed using high-throughput technology. Therefore, the lung-slice system offers broad, current and unrealized potential for the detection of toxicity and the delineation of pathophysiologic and therapeutic mechanisms.
authors.affiliation: Southern Illinois University School of Medicine, 825 North Rutledge St., Springfield, IL 62794, USA. tliberati@siumed.edu
authors.forename: Teresa A
authors.initials: TA
authors.lastname: Liberati
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Randle
authors.forename: Linda A
authors.initials: LA
authors.lastname: Toth
mesh_terms: D000818:Animals; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D008168:Lung / Q000033:anatomy & histology / Q000502:physiology / Q000503:physiopathology; D009924:Organ Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media