title: Functional characterization of bursicon receptor and genome-wide analysis for identification of genes affected by bursicon receptor RNAi.
abstract: Bursicon is an insect neuropeptide hormone that is secreted from the central nervous system into the hemolymph and initiates cuticle tanning. The receptor for bursicon is encoded by the rickets (rk) gene and belongs to the G protein-coupled receptor (GPCR) superfamily. The bursicon and its receptor regulate cuticle tanning as well as wing expansion after adult eclosion. However, the molecular action of bursicon signaling remains unclear. We utilized RNA interference (RNAi) and microarray to study the function of the bursicon receptor (Tcrk) in the model insect, Tribolium castaneum. The data included here showed that in addition to cuticle tanning and wing expansion reported previously, Tcrk is also required for development and expansion of integumentary structures and adult eclosion. Using custom microarrays, we identified 24 genes that are differentially expressed between Tcrk RNAi and control insects. Knockdown in the expression of one of these genes, TC004091, resulted in the arrest of adult eclosion. Identification of genes that are involved in bursicon receptor mediated biological processes will provide tools for future studies on mechanisms of bursicon action.
authors.affiliation: Department of Entomology, S-225 Agriculture Science Bldg N, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Bai
authors.forename: Subba R
authors.initials: SR
authors.lastname: Palli
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D018076:DNA, Complementary / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D016678:Genome; D007447:Invertebrate Hormones / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014231:Tribolium / Q000378:metabolism; D014921:Wings, Animal / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D007447:Invertebrate Hormones; D012330:RNA, Double-Stranded; D043562:Receptors, G-Protein-Coupled; C002295:bursicon
references.citation: J Exp Biol. 2002 Sep;205(Pt 17):2555-65
references.pmid: 12151362
references.citation: Development. 2002 May;129(9):2259-69
references.pmid: 11959833
references.citation: Mech Dev. 2008 Nov-Dec;125(11-12):984-95
references.pmid: 18835439
references.citation: Bioinformatics. 2004 Jun 12;20(9):1453-4
references.pmid: 14871861
references.citation: Development. 2007 Dec;134(24):4395-404
references.pmid: 18003740
references.citation: BMC Genomics. 2008 Sep 18;9:424
references.pmid: 18801173
references.citation: Cell Signal. 1997 Dec;9(8):551-73
references.pmid: 9429760
references.citation: Nucleic Acids Res. 2008 Jun;36(10):3420-35
references.pmid: 18445632
references.citation: Science. 1962 Oct 5;138(3536):27-9
references.pmid: 13894424
references.citation: BMC Bioinformatics. 2005 Mar 21;6:62
references.pmid: 15780134
references.citation: Development. 2003 Jun;130(12):2645-56
references.pmid: 12736209
references.citation: J Neurosci. 2008 Dec 31;28(53):14379-91
references.pmid: 19118171
references.citation: J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2008 Dec;194(12):989-1005
references.pmid: 19005656
references.citation: FEBS Lett. 2007 Feb 20;581(4):697-701
references.pmid: 17270178
references.citation: J Comp Neurol. 2008 Feb 10;506(5):759-74
references.pmid: 18076057
references.citation: J Neurosci. 2006 Jan 11;26(2):573-84
references.pmid: 16407556
references.citation: Genome Res. 2000 Jul;10(7):924-38
references.pmid: 10899142
references.citation: Curr Biol. 2004 Jul 13;14(13):1208-13
references.pmid: 15242619
references.citation: Proc Natl Acad Sci U S A. 2005 Feb 22;102(8):2820-5
references.pmid: 15703293
references.citation: Bioinformatics. 2004 Nov 22;20(17):3246-8
references.pmid: 15180930
references.citation: Nucleic Acids Res. 2003 Aug 15;31(16):e93
references.pmid: 12907745
references.citation: Pharmacol Rev. 2005 Dec;57(4):509-26
references.pmid: 16382105
references.citation: Genetics. 2008 Oct;180(2):885-93
references.pmid: 18780731
references.citation: Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11337-42
references.pmid: 16076951
references.citation: Insect Biochem Mol Biol. 2010 Mar;40(3):267-73
references.pmid: 20080183
references.citation: FEBS Lett. 2005 Apr 11;579(10):2171-6
references.pmid: 15811337
references.citation: Development. 2004 Apr;131(7):1597-606
references.pmid: 14998927