title: Deletion of the N-terminal dirigent domain in maize beta-glucosidase aggregating factor and its homolog sorghum lectin dramatically alters the sugar-specificities of their lectin domains.
abstract: Maize beta-glucosidase aggregating factor (BGAF) and its homolog Sorghum Lectin (SL) are modular proteins consisting of an N-terminal dirigent domain and a C-terminal jacalin-related lectin (JRL) domain. BGAF is a polyspecific lectin with a monosaccharide preference for galactose, whereas SL displays preference for GalNAc. Here, we report that deletion of the N-terminal dirigent domain in the above lectins dramatically changes their sugar-specificities. Deletions in the N-terminal region of the dirigent domain of BGAF abolished binding to galactose/lactose, but binding to mannose was unaffected. Glucose, which was a poor inhibitor of hemagglutinating activity of BGAF, displayed higher inhibitory effect on the hemagglutinating activity of deletion mutants. Deletion of the dirigent domain in SL abolished binding to GalNAc, but binding to mannose was not affected. Surprisingly, fructose, an extremely poor inhibitor (minimum inhibitory concentration (MIC) = 125 mM) of SL hemagglutinating activity, was found to be a very potent inhibitor (MIC = 1 mM) of hemagglutinating activity of its JRL domain. These results indicate that the dirigent domain in this class of modular lectins, at least in the case of maize BGAF and SL, influences sugar specificity.
authors.affiliation: Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
authors.forename: Farooqahmed S
authors.initials: FS
authors.lastname: Kittur
authors.forename: Hyun Young
authors.initials: HY
authors.lastname: Yu
authors.forename: David R
authors.initials: DR
authors.lastname: Bevan
authors.forename: Asim
authors.initials: A
authors.lastname: Esen
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007785:Lactose / Q000378:metabolism*; D037102:Lectins / Q000235:genetics* / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D017384:Sequence Deletion; D045868:Sorghum / Q000235:genetics
chemical_list: C426244:BGAF protein, Zea mays; D002352:Carrier Proteins; D037102:Lectins; D010940:Plant Proteins; D005632:Fructose; D005947:Glucose; D007785:Lactose; D000116:Acetylgalactosamine; D008358:Mannose; D005690:Galactose