title: Expression and function of laminin and integrins on adhesion/migration of primary culture cells derived from rat oral epithelium.
abstract: OBJECTIVE
  It remains controversial whether or not the junctional epithelium cells that are directly attached to teeth migrate on the enamel surface, as those cells are able to adhere firmly to the enamel. The aim of this study was to investigate the expression patterns of laminin gamma(2), integrin beta(4) and integrin alpha(3), and to examine their potential function in cell migration.
  
  
  METHODS
  Oral epithelium cells obtained from Sprague-Dawley rats were established in primary culture. We employed a wound-healing assay to characterize the direction of cell extension at the start of cell migration, and observed different localizations of laminin and integrins using immunofluorescence. For functional analyses of integrins, we employed a phosphatidylinositol-3-kinase (PI3K) activator to promote integrin beta(4) function and used P1B5 to inhibit integrin alpha(3) function, and we analyzed the percentage of re-epithelialization as the migration function.
  
  
  RESULTS
  Marked accumulation of laminin gamma(2) was detected in the peripheral cytoplasm of cells adjacent to the wound area, as shown by the results of the migration assay. Integrin beta(4) was detected in the distal cell processes of actively migrating cells, while integrin alpha(3) was found in cell membranes of cells adjacent to the wound area. In the functional analyses, the percentage of re-epithelialization was significantly lower in the PI3K-activator group and in the P1B5-treated group (2.5% and 7.2%, respectively) than in the control group (39.0%) (p < 0.01).
  
  
  CONCLUSIONS
  The results suggest that laminin gamma(2) is secreted as a foothold for cell migration, that integrin beta(4) participates in cell adhesion and that integrin alpha(3) is involved in cell migration in the primary culture cells.
authors.affiliation: Oral Health Science Center HRC7.
authors.forename: M
authors.initials: M
authors.lastname: Sugisawa
authors.forename: T
authors.initials: T
authors.lastname: Masaoka
authors.forename: Y
authors.initials: Y
authors.lastname: Enokiya
authors.forename: T
authors.initials: T
authors.lastname: Muramatsu
authors.forename: S
authors.initials: S
authors.lastname: Hashimoto
authors.forename: S
authors.initials: S
authors.lastname: Yamada
authors.forename: M
authors.initials: M
authors.lastname: Shimono
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000502:physiology*; D002462:Cell Membrane / Q000648:ultrastructure; D002465:Cell Movement / Q000502:physiology; D002467:Cell Nucleus / Q000648:ultrastructure; D022081:Cell Surface Extensions / Q000648:ultrastructure; D002478:Cells, Cultured; D004396:Coloring Agents; D003593:Cytoplasm / Q000648:ultrastructure; D004789:Enzyme Activation; D004846:Epithelial Attachment / Q000166:cytology* / Q000502:physiology; D004847:Epithelial Cells / Q000502:physiology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D039422:Integrin alpha3 / Q000032:analysis / Q000187:drug effects / Q000502:physiology*; D039663:Integrin beta4 / Q000032:analysis / Q000187:drug effects / Q000502:physiology*; D018613:Microscopy, Confocal; D019869:Phosphatidylinositol 3-Kinases / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000502:physiology; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D004396:Coloring Agents; D039422:Integrin alpha3; D039663:Integrin beta4; D019869:Phosphatidylinositol 3-Kinases; D000097794:Kalinin