Genista tinctoria microsymbionts from Poland are new members of Bradyrhizobium japonicum bv. genistearum.
The phylogeny and taxonomic position of slow-growing Genista tinctoria rhizobia from Poland, Ukraine and England were estimated by comparative 16S rDNA, atpD, and dnaK sequence analyses, PCR-RFLP of 16S rDNA, DNA G+C content, and DNA-DNA hybridization. Each core gene studied placed the G. tinctoria rhizobia in the genus Bradyrhizobium cluster with unequivocal bootstrap support. G. tinctoria symbionts and bradyrhizobial strains shared 96-99% similarity in 16S rDNA sequences. Their similarity for atpD and dnaK sequences was 93-99% and 89-99%, respectively. These data clearly showed that G. tinctoria rhizobia belonged to the genus Bradyrhizobium. 16S rDNA sequence analysis was in good agreement with the results of the PCR-RFLP of the 16S rRNA gene. Although the tested strains formed separate lineages to the reference bradyrhizobia their RFLP 16S rDNA patterns were quite similar. The genomic DNA G+C content of three G. tinctoria rhizobia was in the range from 60.64 to 62.83 mol%. Data for species identification were obtained from DNA-DNA hybridization experiments. G. tinctoria microsymbionts from Poland were classified within Bradyrhizobium japonicum genomospecies based on 56-82% DNA-DNA similarity.
Kalita
Michał
M
Department of Genetics and Microbiology, M. Curie-Skłodowska University, 20-033 Lublin, Poland.
Małek
Wanda
W
D001482:Base Composition; D020369:Bradyrhizobium / Q000235:genetics*; D016000:Cluster Analysis; D055028:Comparative Genomic Hybridization; D005798:Genes, Bacterial / Q000235:genetics; D039761:Genista / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011044:Poland; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013559:Symbiosis
D012336:RNA, Ribosomal, 16S