Redox modulation of Ecto-NOX1 in human platelets.
By modulating the cellular redox state, the plasma membrane electron transport (PMET) is important in platelet biology; indeed, the oxidant/antioxidant balance plays a central role during activation of the coagulation pathway. None the less, in human platelets, the PMET system has not yet been fully characterized and the molecular identities of most components are unknown. Here, for the first time, the presence of the plasma membrane hydroquinone (NADH) oxidase Ecto-NOX1 in human platelets has been described. We found that Ecto-NOX1 expression is modulated by capsaicin: Indeed, it is positively regulated through a mechanism requiring binding of capsaicin to its receptor, namely the transient receptor potential vanilloid subtype 1 (TRPV1). Ligand-receptor interaction triggers a signalling cascade leading to ROS production, which in turn enhances expression and activity of Ecto-NOX1. Redox regulation of Ecto-NOX1 may be important to platelet recruitment and activation during inflammatory diseases.
Savini
Isabella
I
Department of Experimental Medicine & Biochemical Sciences, University of Rome "Tor Vergata", Rome, Italy.
Arnone
Rosaria
R
Rossi
Antonello
A
Catani
M Valeria
MV
Del Principe
Domenico
D
Avigliano
Luciana
L
D000328:Adult; D000704:Analysis of Variance; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology*; D015153:Blotting, Western; D002211:Capsaicin / Q000494:pharmacology; D004579:Electron Transport; D006801:Humans; D008565:Membrane Proteins / Q000097:blood* / Q000737:chemistry; D009243:NAD / Q000097:blood; D009249:NADP / Q000097:blood; D000074624:NADPH Oxidase 1; D019255:NADPH Oxidases / Q000096:biosynthesis / Q000097:blood* / Q000737:chemistry; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D050916:TRPV Cation Channels / Q000097:blood; D055815:Young Adult
D008565:Membrane Proteins; D017382:Reactive Oxygen Species; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; D009243:NAD; D009249:NADP; D000074624:NADPH Oxidase 1; D019255:NADPH Oxidases; C402799:NOX1 protein, human; D002211:Capsaicin