title: Morphology of the cockerel's comb after androgen administration.
abstract: 1. Androgen receptor (AR) expression and morphological changes in blood capillaries were investigated in the comb of cockerels, both untreated controls and after the administration of testosterone (T) or the androgen antagonist flutamide (F) for 7 weeks. 2. Twenty-six male Single Comb White Leghorn roosters were divided into T-treated, F-treated and untreated groups. Tissue sections were examined histologically, immunohistochemically, and comb blood vessel castings were analysed by scanning electron microscopy. 3. Histologically, the capillaries of the peripheral dermis layer in the T-treated group were dilated compared with controls. Many red blood cells were seen in the lumen. Although the capillary diameter in the F-treated group did not show a significant difference as compared with control, blood capillaries with small diameters were often observed, and there were few red blood cells in the capillaries. Some capillary castings were extended markedly in the T treated group, and small blood vessels were observed arborising from the extended blood capillaries. In contrast, all capillaries were slender in the F-treated group, and the casting surface was rough. 4. Immunoreactivity for AR was found in capillary endothelial cells in the peripheral dermis layer of the comb. The intensity of staining in these cells was increased in the T-treated group but was reduced in the F-treated group. 5. It is concluded that immunoreactivity for AR was found in capillary endothelial cells in the peripheral dermis layer of the rooster's comb. The intensity of staining in these cells was increased in the T-treated group but reduced in the F-treated group. Thus, the capillary endothelial cells in the peripheral dermis layer of the comb are androgen targets.
authors.affiliation: Department of Veterinary Anatomy, School of Veterinary Medicine, Kitasato University, Aomori, Japan. yoshioka@vmas.kitasato-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Yoshioka
authors.forename: Y
authors.initials: Y
authors.lastname: Watahiki
authors.forename: A
authors.initials: A
authors.lastname: Kanie
authors.forename: M
authors.initials: M
authors.lastname: Tsujio
authors.forename: H
authors.initials: H
authors.lastname: Ikadai
authors.forename: T
authors.initials: T
authors.lastname: Kashimoto
authors.forename: K
authors.initials: K
authors.lastname: Mutoh
mesh_terms: D000726:Androgen Antagonists / Q000494:pharmacology; D000728:Androgens / Q000494:pharmacology; D000818:Animals; D002645:Chickens / Q000033:anatomy & histology* / Q000502:physiology*; D003129:Comb and Wattles / Q000254:growth & development*; D005485:Flutamide / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D000726:Androgen Antagonists; D000728:Androgens; D011944:Receptors, Androgen; D013739:Testosterone; D005485:Flutamide