title: Multimodal nonlinear optical imaging of atherosclerotic plaque development in myocardial infarction-prone rabbits.
abstract: Label-free imaging of bulk arterial tissue is demonstrated using a multimodal nonlinear optical microscope based on a photonic crystal fiber and a single femtosecond oscillator operating at 800 nm. Colocalized imaging of extracellular elastin fibers, fibrillar collagen, and lipid-rich structures within aortic tissue obtained from atherosclerosis-prone myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits is demonstrated through two-photon excited fluorescence, second harmonic generation, and coherent anti-Stokes Raman scattering, respectively. These images are shown to differentiate healthy arterial wall, early atherosclerotic lesions, and advanced plaques. Clear pathological changes are observed in the extracellular matrix of the arterial wall and correlated with progression of atherosclerotic disease as represented by the age of the WHHLMI rabbits.
authors.affiliation: National Research Council Canada, Institute for Biodiagnostics, 435 Ellice Avenue, Winnipeg, Manitoba, Canada R3B 1Y6. alex.ko@nrc-cnrc.gc.ca
authors.forename: Alex C T
authors.initials: AC
authors.lastname: Ko
authors.forename: Andrew
authors.initials: A
authors.lastname: Ridsdale
authors.forename: Michael S D
authors.initials: MS
authors.lastname: Smith
authors.forename: Leila B
authors.initials: LB
authors.lastname: Mostaço-Guidolin
authors.forename: Mark D
authors.initials: MD
authors.lastname: Hewko
authors.forename: Adrian F
authors.initials: AF
authors.lastname: Pegoraro
authors.forename: Elicia K
authors.initials: EK
authors.lastname: Kohlenberg
authors.forename: Bernie
authors.initials: B
authors.lastname: Schattka
authors.forename: Masashi
authors.initials: M
authors.lastname: Shiomi
authors.forename: Albert
authors.initials: A
authors.lastname: Stolow
authors.forename: Michael G
authors.initials: MG
authors.lastname: Sowa
mesh_terms: D000818:Animals; D003324:Coronary Artery Disease / Q000150:complications* / Q000473:pathology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D018613:Microscopy, Confocal / Q000295:instrumentation*; D009203:Myocardial Infarction / Q000209:etiology* / Q000473:pathology*; D017711:Nonlinear Dynamics; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity