title: Ultrasensitive aptamer-based protein detection via a dual amplified biocatalytic strategy.
abstract: We present an ultrasensitive aptasensor for the electronic monitoring of proteins through a dual amplified strategy in this paper. The target protein thrombin is sandwiched between an electrode surface confined aptamer and an aptamer-enzyme-carbon nanotube bioconjugate. The analytical signal amplification is achieved by coupling the signal amplification nature of multiple enzymes with the biocatalytic signal enhancement of redox-recycling. Our novel dramatic signal amplification strategy, with a detection limit of 8.3fM, shows about 4 orders of magnitude improvement in the sensitivity for thrombin detection compared to other universal single enzyme-based assay. This makes our approach an attractive alternative to other common PCR-based signal amplification in ultralow level of protein detection.
authors.affiliation: Key Laboratory of Ministry of Education on Luminescence and Real-Time Analysis, School of Chemistry and Chemical Engineering, Southwest University, #2 Tiansheng Road, Chongqing 400715, PR China. yunatswu@swu.edu.cn
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.forename: Yuyong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Qian
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.forename: Joseph
authors.initials: J
authors.lastname: Wang
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
mesh_terms: D052158:Aptamers, Peptide / Q000737:chemistry*; D002384:Catalysis; D004867:Equipment Design; D019544:Equipment Failure Analysis; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D052158:Aptamers, Peptide; D011506:Proteins
references.citation: Analyst. 2005 Apr;130(4):421-6
references.pmid: 15846872
references.citation: Chem Rev. 2005 Apr;105(4):1547-62
references.pmid: 15826019
references.citation: Nature. 2002 Dec 19-26;420(6917):761
references.pmid: 12490938
references.citation: Anal Chem. 2006 Jun 1;78(11):3638-42
references.pmid: 16737218
references.citation: J Am Chem Soc. 2006 Aug 30;128(34):11199-205
references.pmid: 16925438
references.citation: Biosens Bioelectron. 2005 Apr 15;20(10):2168-72
references.pmid: 15741093
references.citation: Biosens Bioelectron. 2004 Dec 15;20(6):1149-56
references.pmid: 15556361
references.citation: Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9134-7
references.pmid: 12867594
references.citation: Angew Chem Int Ed Engl. 2005 Aug 26;44(34):5456-9
references.pmid: 16044476
references.citation: Angew Chem Int Ed Engl. 2004 Aug 27;43(34):4519-22
references.pmid: 15340959
references.citation: Anal Chem. 2005 Oct 1;77(19):6320-3
references.pmid: 16194094
references.citation: J Am Chem Soc. 2007 Apr 4;129(13):3808-9
references.pmid: 17355135
references.citation: Angew Chem Int Ed Engl. 2006 Feb 27;45(10):1576-80
references.pmid: 16440380
references.citation: Angew Chem Int Ed Engl. 2008;47(34):6330-7
references.pmid: 18680110
references.citation: Angew Chem Int Ed Engl. 2007;46(34):6408-18
references.pmid: 17600802
references.citation: Anal Chem. 2007 Feb 15;79(4):1466-73
references.pmid: 17297945
references.citation: J Am Chem Soc. 2004 Mar 17;126(10):3010-1
references.pmid: 15012105
references.citation: Small. 2005 Nov;1(11):1036-43
references.pmid: 17193390
references.citation: Anal Bioanal Chem. 2009 May;394(1):207-14
references.pmid: 19148626
references.citation: Anal Chem. 2004 Jul 15;76(14):4066-75
references.pmid: 15253644
references.citation: J Am Chem Soc. 2005 May 11;127(18):6522-3
references.pmid: 15869259
references.citation: J Am Chem Soc. 2005 Dec 28;127(51):17990-1
references.pmid: 16366535
references.citation: J Am Chem Soc. 2006 May 10;128(18):6014-5
references.pmid: 16669652
references.citation: Anal Chem. 2007 Oct 1;79(19):7492-500
references.pmid: 17722881
references.citation: Analyst. 2008 Nov;133(11):1599-604
references.pmid: 18936839
references.citation: J Am Chem Soc. 2008 Jun 11;130(23):7276-85
references.pmid: 18491854
references.citation: J Am Chem Soc. 2008 Aug 27;130(34):11268-9
references.pmid: 18680291
references.citation: J Am Chem Soc. 2006 Feb 22;128(7):2228-9
references.pmid: 16478173
references.citation: Biosens Bioelectron. 2007 Sep 30;23(2):218-24
references.pmid: 17512185
references.citation: Anal Chem. 1996 Aug 1;68(15):2453-8
references.pmid: 8694255
references.citation: Biosens Bioelectron. 2003 Aug 15;18(9):1095-100
references.pmid: 12788551
references.citation: Angew Chem Int Ed Engl. 2007;46(47):9054-6
references.pmid: 17957666
references.citation: Anal Chem. 2005 Jul 15;77(14):4662-6
references.pmid: 16013886
references.citation: Biosens Bioelectron. 2009 Jun 15;24(10):2961-6
references.pmid: 19345084