Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation.
Boron nitride nanotubes (BNNTs) have generated considerable interest within the scientific community by virtue of their unique physical properties, which can be exploited in the biomedical field. In the present in vitro study, we investigated the interactions of poly-l-lysine-coated BNNTs with C2C12 cells, as a model of muscle cells, in terms of cytocompatibility and BNNT internalization. The latter was performed using both confocal and transmission electron microscopy. Finally, we investigated myoblast differentiation in the presence of BNNTs, evaluating the protein synthesis of differentiating cells, myotube formation, and expression of some constitutive myoblastic markers, such as MyoD and Cx43, by reverse transcription - polymerase chain reaction and Western blot analysis. We demonstrated that BNNTs are highly internalized by C2C12 cells, with neither adversely affecting C2C12 myoblast viability nor significantly interfering with myotube formation.
Ciofani
G
G
Scuola Superiore Sant'Anna, Pisa, Italy.
Ricotti
L
L
Danti
S
S
Moscato
S
S
Nesti
C
C
D'Alessandro
D
D
Dinucci
D
D
Chiellini
F
F
Pietrabissa
A
A
Petrini
M
M
Menciassi
A
A
D000818:Animals; D001896:Boron Compounds / Q000008:administration & dosage* / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage* / Q000737:chemistry*; D008422:Materials Testing; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D011107:Polylysine / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats
D001896:Boron Compounds; D020099:Coated Materials, Biocompatible; D011107:Polylysine; C017282:boron nitride
Methods Enzymol. 1990;182:50-68
2314256
Nano Lett. 2007 Mar;7(3):632-7
17288485
J Am Chem Soc. 2005 Dec 14;127(49):17144-5
16332036
J Thorac Cardiovasc Surg. 2001 Oct;122(4):759-66
11581610
J Nanobiotechnology. 2007 Oct 23;5:8
17956629
Chem Asian J. 2007 Dec 3;2(12):1581-5
18041790
Biochem Biophys Res Commun. 2009 May 1;382(2):473-7
19289111
Science. 1995 Aug 18;269(5226):966-7
17807732
J Exp Med. 1995 Nov 1;182(5):1545-56
7595224
Dev Biol. 2009 Aug 1;332(1):131-41
19464281
Mol Biol Cell. 2007 May;18(5):1734-43
17332503
J Nanosci Nanotechnol. 2008 Dec;8(12):6223-31
19205187
Nanoscale Res Lett. 2008 Nov 25;4(2):113-121
20596476
Adv Drug Deliv Rev. 2006 Dec 1;58(14):1460-70
17113677
Biotechnol Bioeng. 2008 Nov 1;101(4):850-8
18512259
Life Sci. 2009 Mar 27;84(13-14):415-20
19302823
Nanotechnology. 2009 Jun 24;20(25):255101
19487801
Angew Chem Int Ed Engl. 2006 Jan 16;45(4):577-81
16345107
Am J Physiol Endocrinol Metab. 2008 Nov;295(5):E1191-204
18780777
Anal Biochem. 1997 Jul 1;249(2):228-38
9212875