title: Fine mapping of the soybean aphid-resistance gene Rag2 in soybean PI 200538.
abstract: The discovery of biotype diversity of soybean aphid (SA: Aphis glycines Matsumura) in North America emphasizes the necessity to identify new aphid-resistance genes. The soybean [Glycine max (L.) Merr.] plant introduction (PI) 200538 is a promising source of SA resistance because it shows a high level of resistance to a SA biotype that can overcome the SA-resistance gene Rag1 from 'Dowling'. The SA-resistance gene Rag2 was previously mapped from PI 200538 to a 10-cM marker interval on soybean chromosome 13 [formerly linkage group (LG) F]. The objective of this study was to fine map Rag2. This fine mapping was carried out using lines derived from 5,783 F(2) plants at different levels of backcrossing that were screened with flanking genetic markers for the presence of recombination in the Rag2 interval. Fifteen single nucleotide polymorphism (SNP) markers and two dominant polymerase chain reaction-based markers near Rag2 were developed by re-sequencing target intervals and sequence-tagged sites. These efforts resulted in the mapping of Rag2 to a 54-kb interval on the Williams 82 8x assembly (Glyma1). This Williams 82 interval contains seven predicted genes, which includes one nucleotide-binding site-leucine-rich repeat gene. SNP marker and candidate gene information identified in this study will be an important resource in marker-assisted selection for aphid resistance and for cloning the gene.
authors.affiliation: Department of Crop Sciences, University of Illinois, Urbana, IL 61801, USA.
authors.forename: Ki-Seung
authors.initials: KS
authors.lastname: Kim
authors.forename: Curtis B
authors.initials: CB
authors.lastname: Hill
authors.forename: Glen L
authors.initials: GL
authors.lastname: Hartman
authors.forename: David L
authors.initials: DL
authors.lastname: Hyten
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Hudson
authors.forename: Brian W
authors.initials: BW
authors.lastname: Diers
mesh_terms: D000818:Animals; D001042:Aphids / Q000502:physiology*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers; D007113:Immunity, Innate / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013025:Glycine max / Q000235:genetics* / Q000276:immunology / Q000469:parasitology
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers
references.citation: Theor Appl Genet. 2002 May;104(6-7):1055-1063
references.pmid: 12582612
references.citation: J Econ Entomol. 2004 Jun;97(3):1071-7
references.pmid: 15279293
references.citation: Theor Appl Genet. 2010 Mar;120(5):1063-71
references.pmid: 20035316
references.citation: Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9750-4
references.pmid: 9707547
references.citation: Plant Physiol. 2005 Apr;137(4):1445-55
references.pmid: 15778464
references.citation: J Econ Entomol. 2010 Apr;103(2):509-15
references.pmid: 20429468
references.citation: Mol Plant Microbe Interact. 2003 Sep;16(9):817-26
references.pmid: 12971605
references.citation: Environ Entomol. 2009 Aug;38(4):1301-11
references.pmid: 19689913
references.citation: Theor Appl Genet. 1995 Jul;91(1):178-88
references.pmid: 24169684
references.citation: Mol Gen Genet. 1991 May;226(3):377-82
references.pmid: 1674815
references.citation: Genetics. 1996 Sep;144(1):329-38
references.pmid: 8878696
references.citation: Nature. 2010 Jan 14;463(7278):178-83
references.pmid: 20075913
references.citation: New Phytol. 2008;179(1):185-195
references.pmid: 18422900
references.citation: J Econ Entomol. 2004 Jun;97(3):1106-11
references.pmid: 15279297
references.citation: Annu Rev Plant Biol. 2003;54:23-61
references.pmid: 14502984
references.citation: Theor Appl Genet. 2009 Feb;118(3):473-82
references.pmid: 18982306
references.citation: Mol Plant Microbe Interact. 2005 Dec;18(12):1285-95
references.pmid: 16478048
references.citation: Genetics. 2007 May;176(1):685-96
references.pmid: 17339218
references.citation: Theor Appl Genet. 2008 Oct;117(6):955-62
references.pmid: 18626623
references.citation: Plant Cell. 1998 Aug;10(8):1307-19
references.pmid: 9707531
references.citation: Genetics. 1999 Dec;153(4):1929-48
references.pmid: 10581297
references.citation: Theor Appl Genet. 2004 Jun;109(1):122-8
references.pmid: 14991109