Wheat dehydrin DHN-5 exerts a heat-protective effect on beta-glucosidase and glucose oxidase activities.
Group-2 late embryogenesis abundant (LEA) proteins, also known as dehydrins, are claimed to stabilize macromolecules against damage caused by freezing, dehydration, ionic or osmotic stresses. However, their precise function remains unknown. Here, we investigated the effect of wheat dehydrin (DHN-5) protein on the activity and thermostability of two distinct enzymes, beta-glucosidase (bglG) and glucose oxidase/peroxidase (GOD/POD) in vitro. The purified DHN-5 protein had the capacity to preserve and stabilize the activity of bglG subjected to heat treatment. In addition, DHN-5 stabilized oxidizing enzymes, as it improved reliability in measuring glucose concentrations with a glucose oxidase/peroxidase (GOD/POD) kit while the temperature increased from 37 to 70 degrees C. All together the data presented provide evidence that DHN-5 is a dehydrin able to preserve enzyme activities in vitro from adverse effects induced by heating.
Brini
Faiçal
F
Plant Molecular Genetics Laboratory, Centre of Biotechnology of Sfax, Sfax, Tunisia.
Saibi
Walid
W
Amara
Imen
I
Gargouri
Ali
A
Masmoudi
Khaled
K
Hanin
Moez
M
D004789:Enzyme Activation; D004795:Enzyme Stability; D005949:Glucose Oxidase / Q000378:metabolism*; D006358:Hot Temperature; D010544:Peroxidases / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D013191:Stachybotrys / Q000201:enzymology; D013312:Stress, Physiological; D014908:Triticum; D001617:beta-Glucosidase / Q000378:metabolism*
D010940:Plant Proteins; D011994:Recombinant Proteins; C070556:dehydrin proteins, plant; D005949:Glucose Oxidase; D010544:Peroxidases; C023057:glucose peroxidase; D001617:beta-Glucosidase