title: Natural killer cell cytotoxicity and immunosuppressive cytokines (IL-10, TGF-beta1) in patients with gastric cancer.
abstract: Cytotoxic activity of NK cells was estimated as related to IL-10 and TGF-beta1 serum levels and Helicobacter pylori infection in gastric cancer patients. Moreover, we sought to determine whether human gastric adenocarcinoma cells in vitro release IL-10, TGF-beta1 or factor(s) affecting NK cytotoxicity. The studies were conducted on 42 patients with gastric cancer (14 with I-II stage-group 1; 28 with III-IV stage-group 2) and on 20 healthy volunteers. The cytotoxicity was tested on NK cells isolated from peripheral blood. IL-10 and TGF-beta1 levels were determined by ELISA. H. pylori was detected in cultures of gastric mucosa biopsies and in direct preparations. In 71.4% patients of group 1 NK cytotoxicity and IL-10 serum levels remained within a normal range while in 68% patients of group 2 a marked decrease was noted in cytotoxic function of NK cells, accompanied by increased levels of IL-10 in serum. In turn, in most patients of either group, independently of NK cytotoxicity and stage grouping in the patients, elevated serum levels of TGF-beta1 were detected. Presence of H. pylori infection manifested no relationship with NK cytotoxicity, IL-10, or the TGF-beta1 serum levels. In cultures of tumour cells presence of IL-10 and TGF-beta1 was demonstrated. Nevertheless, supernatants of the cultures did not change cytotoxic activity of NK cells. Development of gastric carcinoma is accompanied by markedly decreased cytotoxic function of NK cells and by elevated IL-10 and TGF-beta1 serum levels. Gastric carcinoma cells may release IL-10, the suppressive activity of which may in a secondary manner decrease NK cytotoxicity.
authors.affiliation: Department of Medical Microbiology, University of Medical Sciences in Poznań, Wieniawskiego 3 Street, 61-712 Poznań, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Szkaradkiewicz
authors.forename: Tomasz M
authors.initials: TM
authors.lastname: Karpiński
authors.forename: Michał
authors.initials: M
authors.lastname: Drews
authors.forename: Maciej
authors.initials: M
authors.lastname: Borejsza-Wysocki
authors.forename: Przemysław
authors.initials: P
authors.lastname: Majewski
authors.forename: Ewa
authors.initials: E
authors.lastname: Andrzejewska
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D003602:Cytotoxicity, Immunologic; D005260:Female; D016481:Helicobacter Infections / Q000097:blood / Q000276:immunology; D016480:Helicobacter pylori; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018709:Statistics, Nonparametric; D013274:Stomach Neoplasms / Q000097:blood / Q000276:immunology*; D053773:Transforming Growth Factor beta1 / Q000097:blood / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: C508609:IL10 protein, human; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10
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