title: Q-FISH analysis of telomere and chromosome instability in the oesophagus with and without squamous cell carcinoma in situ.
abstract: Chromosomal and genomic instability due to telomere dysfunction is known to play an important role in carcinogenesis. To study telomere dysfunction in the surrounding background epithelium of squamous cell carcinoma in situ (CIS) of the oesophagus, we measured telomere lengths of basal and parabasal cells of epithelia with and without CIS using quantitative fluorescence in situ hybridization (Q-FISH) and our original software, Tissue Telo. Additionally, we assessed histological inflammation and the anaphase bridge index. In non-cancerous epithelium, telomeres in basal cells were significantly longer than those in parabasal cells, whereas CIS showed a homogeneous telomere pattern in the basal and parabasal cells. Telomeres in basal and parabasal cells were significantly shorter in the background with CIS than in epithelium from age-matched normal controls. Significant negative correlation was observed between the normalized telomere : centromere ratio (reflected telomere length) and the anaphase bridge index in non-cancerous epithelia from both normal controls and the CIS background with no histological inflammation. These findings indicate that tissue stem cells may be located among basal cells, and that telomere length distribution in component cell types differs between CIS and non-cancerous epithelium. We have demonstrated conclusively that oesophageal CIS arises from epithelium with short telomeres and chromosomal instability in the absence of histological inflammation.
authors.affiliation: Research Team for Geriatric Pathology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015, Japan. takubo@tmig.or.jp
authors.forename: Kaiyo
authors.initials: K
authors.lastname: Takubo
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fujita
authors.forename: Naotaka
authors.initials: N
authors.lastname: Izumiyama
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Nakamura
authors.forename: Naoshi
authors.initials: N
authors.lastname: Ishikawa
authors.forename: Steven S
authors.initials: SS
authors.lastname: Poon
authors.forename: Mutsunori
authors.initials: M
authors.lastname: Fujiwara
authors.forename: Motoji
authors.initials: M
authors.lastname: Sawabe
authors.forename: Masaaki
authors.initials: M
authors.lastname: Matsuura
authors.forename: Heike
authors.initials: H
authors.lastname: Grabsch
authors.forename: Tomio
authors.initials: T
authors.lastname: Arai
authors.forename: Junko
authors.initials: J
authors.lastname: Aida
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002278:Carcinoma in Situ / Q000235:genetics* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D043171:Chromosomal Instability / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D008875:Middle Aged; D016615:Telomere / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured