[Effect of alpha-naphthlcetic acid on the proliferation and apoptosis of testis cell].
OBJECTIVE
To investigate the effect of alpha-naphthlcetic acid on the proliferation and apoptosis of testis cell.


METHODS
Twenty five mice were divided into five groups, 5 mice per group. Basal animal food was fed to the mice in the normal control and positive control groups. Five thousand, 1000, 200 mg/kg of alpha-naphthlcetic acid were added into another three groups. Three days before sacrifice, the mice in positive control group were intraperitoneally injected with cyclophosphamide of 20 mg/kg each day. Four weeks later, all the mice were sacrificed. The proliferation activity of the testis cells was determined by MTT assay, and the levels of Caspase-3 and PCNA protein expression in testis cell were measured by immunohistochemistry method.


RESULTS
The testis cell proliferation activity of the high dose group and the normal control group were 0.501 +/- 0.069 and 0.875 +/- 0.082. The difference was significant (P < 0.05). The expression level of PCNA protein and Caspase-3 of the high dose group were 8.9% and 41.3%, respectively, and those of the normal control group were 34.9% and 9.1%. The differences between two groups were significant (P < 0.05).


CONCLUSIONS
alpha-naphthlcetic acid can inhibit the proliferation and induce the apoptosis of testis cells effectively.
Sun
Qiaoping
Q
Medical College of Qingdao University, Qingdao 266021, China. qpsun0532@126.com
Zhong
Jinyi
J
Li
Lei
L
Cui
Jing
J
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D008297:Male; D051379:Mice; D009280:Naphthaleneacetic Acids / Q000633:toxicity*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011897:Random Allocation; D013737:Testis / Q000166:cytology*
D009280:Naphthaleneacetic Acids; D018809:Proliferating Cell Nuclear Antigen; C505409:Casp3 protein, mouse; D053148:Caspase 3