Induction of oxidative stress and cytotoxicity by PCB126 in JEG-3 human choriocarcinoma cells.
The compound 3,3',4,4',5-pentachlorobiphenyl (PCB126) exists in various environmental media, which may have adverse effects on human health. In the present study, induction of the oxidative stress and cytotoxicity by PCB126 were investigated in the human choriocarcinoma cell line JEG-3 cells. Both the reactive oxygen species (ROS) generation and lipid peroxidation production of malondialdehyde (MDA) were obviously increased. Whereas the activity of antioxidant enzymes (superoxide dismutase [SOD] and catalase [CAT]) and glutathione (GSH) content were declined with dose-dependent manners. Furthermore, the result of the cytotoxicity assay showed a clear, dose-dependent growth inhibition effect of PCB126 in JEG-3 cells. Our present results revealed that PCB126 exhibited significant oxidative stress and cytotoxicity in human trophoblast. Given the widespread use of PCBs, a more comprehensive understanding of the significance of reproductive toxicity of PBCs is imperative for improving risk assessment and regulation of these chemicals.
Chen
Fang
F
College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, China.
D002374:Catalase / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002822:Choriocarcinoma / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D017382:Reactive Oxygen Species; D011078:Polychlorinated Biphenyls; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; C023035:3,4,5,3',4'-pentachlorobiphenyl