The impact of larval and adult dietary restriction on lifespan, reproduction and growth in the mosquito Aedes aegypti.
Dietary restriction extends lifespan in many organisms, but little is known about how it affects hematophagous arthropods. We demonstrated that diet restriction during either larval or adult stages extends Aedes aegypti lifespan. A. aegypti females fed either single or no blood meals survived 30-40% longer than those given weekly blood meals. However, mosquitoes given weekly blood meals produced far more eggs. To minimize reproduction's impact on lifespan, adult mosquitoes were fed artificial blood meals containing <10% of the protein in normal human blood, minimizing egg production. A. aegypti fed artificial blood meals containing 25mg/ml of BSA had significantly shorter lifespans than those fed either 10 or 5mg/ml. To assess the impact of larval dietary restriction on adult lifespan, we maintained larval A. aegypti on 2X, 1X (normal diet), 0.5X or 0.25X diets. Adult mosquitoes fed 0.5X and 0.25X larval diets survived significantly longer than those fed the 2X larval diet regardless of adult diet. In summary, dietary restriction during both larval and adult stages extends lifespan. This diet-mediated lifespan extension has important consequences for understanding how dietary restriction regulates lifespan and disease transmission.
Joy
Teresa K
TK
Department of Entomology, University of Arizona, Tucson, AZ 85721, United States.
Arik
Anam J
AJ
Corby-Harris
Vanessa
V
Johnson
Adiv A
AA
Riehle
Michael A
MA
D000330:Aedes / Q000254:growth & development* / Q000502:physiology; D000818:Animals; D001769:Blood; D004032:Diet; D004038:Diet, Reducing; D002149:Energy Intake; D005260:Female; D006801:Humans; D007814:Larva / Q000502:physiology*; D008017:Life Expectancy; D008136:Longevity / Q000502:physiology*; D008297:Male; D012098:Reproduction / Q000502:physiology*
J Med Entomol. 1998 Sep;35(5):809-13
9775613
Aging Cell. 2003 Dec;2(6):327-33
14677635
Annu Rev Biochem. 2008;77:727-54
18373439
Aging Cell. 2002 Dec;1(2):140-8
12882344
J Med Entomol. 1993 Sep;30(5):922-7
8254642
Acta Vet Scand. 2007 Nov 09;49:33
17996082
PLoS Biol. 2005 Jul;3(7):e223
16000018
Exp Gerontol. 2009 Jun-Jul;44(6-7):413-9
19328225
PLoS One. 2009;4(1):e4067
19119322
Med Vet Entomol. 2006 Mar;20(1):53-9
16608490
Science. 1928 May 18;67(1742):515-6
17771549
Am J Trop Med Hyg. 2007 Jan;76(1):111-7
17255238
PLoS Genet. 2007 Apr 27;3(4):e57
17465680
Am J Trop Med Hyg. 1978 Jul;27(4):827-31
686250
J Gerontol A Biol Sci Med Sci. 1996 Jul;51(4):B280-3
8680993
Exp Gerontol. 2004 Jul;39(7):1011-9
15236760
Vector Borne Zoonotic Dis. 2007 Spring;7(1):86-98
17417961
Med Vet Entomol. 2009 Mar;23(1):62-8
19239615
Mech Ageing Dev. 2005 Sep;126(9):938-50
15935441
J Med Entomol. 1997 Nov;34(6):589-93
9439110
J Med Entomol. 2001 Jul;38(4):537-47
11476334
FASEB J. 2004 Oct;18(13):1591-3
15319362
J Am Mosq Control Assoc. 2008 Dec;24(4):496-501
19181055