title: Transaminations with isopropyl amine: equilibrium displacement with yeast alcohol dehydrogenase coupled to in situ cofactor regeneration.
abstract: Enantiopure chiral amines synthesis using omega-transaminases is hindered by an unfavourable equilibrium, but when using isopropylamine as the amine donor the equilibrium can be completely displaced by using a specific dehydrogenase in situ for removal of formed acetone.
authors.affiliation: Division of Biochemistry, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, SE-106 91 Stockholm, Sweden.
authors.forename: Karim Engelmark
authors.initials: KE
authors.lastname: Cassimjee
authors.forename: Cecilia
authors.initials: C
authors.lastname: Branneby
authors.forename: Vahak
authors.initials: V
authors.lastname: Abedi
authors.forename: Andrew
authors.initials: A
authors.lastname: Wells
authors.forename: Per
authors.initials: P
authors.lastname: Berglund
mesh_terms: D000096:Acetone / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000586:Amination; D000588:Amines / Q000138:chemical synthesis* / Q000378:metabolism; D011437:Propylamines / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013237:Stereoisomerism
chemical_list: D000588:Amines; D011437:Propylamines; D000096:Acetone; D000426:Alcohol Dehydrogenase; C035263:2-propylamine