title: Affinity and specificity of ciprofloxacin-bovine serum albumin interactions: spectroscopic approach.
abstract: Fluorescence spectroscopy in combination with UV-Vis absorption spectroscopy were employed to investigate the binding of an antibacterial drug Ciprofloxacin (CPFX) to bovine serum albumin (BSA) under the physiological conditions. In the discussion of the quenching mechanism, it was proved that the fluorescence quenching of BSA by CPFX is a result of the formation of CPFX-BSA complex. Binding parameters were determined using the modified Stern-Volmer equation and Scatchard equation to provide a measure of the binding affinity between CPFX and BSA. The results of thermodynamic parameters DeltaG, DeltaH, DeltaS, at different temperatures indicate that the electrostatic interactions play a major role for CPFX-BSA association. Site marker competitive experiments indicated that the binding of CPFX to BSA primarily took place in site I. Furthermore, the effect of metal ions to CPFX-BSA system was studied, and the distance r between donor (BSA) and acceptor (CPFX) was obtained according to fluorescence resonance energy transfer (FRET). The conformation of BSA upon CPFX binding was evaluated by measuring synchronous fluorescence properties of the CPFX-BSA complex.
authors.affiliation: Department of Chemistry, Hubei Normal University, Huangshi, People's Republic of China. yjhu@263.net
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Hu
authors.forename: Yu
authors.initials: Y
authors.lastname: Ou-Yang
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000465:Algorithms; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002939:Ciprofloxacin / Q000737:chemistry* / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature; D013816:Thermodynamics
chemical_list: D012710:Serum Albumin, Bovine; D002939:Ciprofloxacin
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