Characterization of the transcriptome of chorioamniotic membranes at the site of rupture in spontaneous labor at term.
OBJECTIVE
The purpose of this study was to compare the transcriptome between the site of membrane rupture and the chorioamniotic membranes away from the site of rupture.


METHODS
The transcriptome of amnion and chorion (n=20 each) from and distal to the site of rupture from women with spontaneous labor and vaginal delivery at term after spontaneous rupture of membranes was profiled with Illumina HumanHT-12 microarrays. Selected genes were validated with the use of quantitative reverse transcription-polymerase chain reaction.


RESULTS
Six hundred seventy-seven genes were differentially expressed in the chorion between the rupture and nonrupture sites (false discovery rate<0.1; fold change>1.5). Quantitative reverse transcription-polymerase chain reaction confirmed the differential expression in 10 of 14 genes. Enriched biological processes included anatomic structure development, cell adhesion and signal transduction. Extracellular matrix-receptor interaction was the most impacted signaling pathway.


CONCLUSIONS
The transcriptome of fetal membranes after spontaneous rupture of membranes in term labor is characterized by region- and tissue-specific differential expression of genes that are involved in signature pathways, which include extracellular matrix-receptor interactions.
Nhan-Chang
Chia-Ling
CL
Perinatology Research Branch, Intramural Division, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NICHD/NIH/DHHS, Bethesda, MD, USA.
Romero
Roberto
R
Tarca
Adi L
AL
Mittal
Pooja
P
Kusanovic
Juan Pedro
JP
Erez
Offer
O
Mazaki-Tovi
Shali
S
Chaiworapongsa
Tinnakorn
T
Hotra
John
J
Than
Nandor Gabor
NG
Kim
Jung-Sun
JS
Hassan
Sonia S
SS
Kim
Chong Jai
CJ
D000328:Adult; D000650:Amnion / Q000502:physiology; D002823:Chorion / Q000502:physiology; D005321:Extraembryonic Membranes / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007743:Labor, Obstetric / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D011446:Prospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D055815:Young Adult
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