title: Expression and crystallization of SeDsbA, SeDsbL and SeSrgA from Salmonella enterica serovar Typhimurium.
abstract: Pathogens require protein-folding enzymes to produce functional virulence determinants. These foldases include the Dsb family of proteins, which catalyze oxidative folding in bacteria. Bacterial disulfide catalytic processes have been well characterized in Escherichia coli K-12 and these mechanisms have been extrapolated to other organisms. However, recent research indicates that the K-12 complement of Dsb proteins is not common to all bacteria. Importantly, many pathogenic bacteria have an extended arsenal of Dsb catalysts that is linked to their virulence. To help to elucidate the process of oxidative folding in pathogens containing a wide repertoire of Dsb proteins, Salmonella enterica serovar Typhimurium has been focused on. This Gram-negative bacterium contains three DsbA proteins: SeDsbA, SeDsbL and SeSrgA. Here, the expression, purification, crystallization and preliminary diffraction analysis of these three proteins are reported. SeDsbA, SeDsbL and SeSrgA crystals diffracted to resolution limits of 1.55, 1.57 and 2.6 A and belonged to space groups P2(1), P2(1)2(1)2 and C2, respectively.
authors.affiliation: The University of Queensland, Institute for Molecular Bioscience, QLD 4072, Australia.
authors.forename: R
authors.initials: R
authors.lastname: Jarrott
authors.forename: S R
authors.initials: SR
authors.lastname: Shouldice
authors.forename: G
authors.initials: G
authors.lastname: Guncar
authors.forename: M
authors.initials: M
authors.lastname: Totsika
authors.forename: M A
authors.initials: MA
authors.lastname: Schembri
authors.forename: B
authors.initials: B
authors.lastname: Heras
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D019704:Protein Disulfide-Isomerases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D050862:Oxidoreductases Acting on Sulfur Group Donors; C490374:SrgA protein, Salmonella typhimurium; D019704:Protein Disulfide-Isomerases
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