title: Detection of pathogenic Streptococcus suis bacteria using magnetic glycoparticles.
abstract: Detection of the zoonotic bacterial pathogen Streptococcus suis was achieved using magnetic glycoparticles. The bacteria contain an adhesion protein for the carbohydrate sequence Galalpha1,4Gal. After incubation with various amounts of the pathogen, magnetic concentration and ATP detection, bacterial levels down to 10(5) cfu could be detected. Submicrometer particles were needed, since with the larger microparticles the method did not succeed.
authors.affiliation: Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands.
authors.forename: Núria Parera
authors.initials: NP
authors.lastname: Pera
authors.forename: Annika
authors.initials: A
authors.lastname: Kouki
authors.forename: Sauli
authors.initials: S
authors.lastname: Haataja
authors.forename: Hilbert M
authors.initials: HM
authors.lastname: Branderhorst
authors.forename: Rob M J
authors.initials: RM
authors.lastname: Liskamp
authors.forename: Gerben M
authors.initials: GM
authors.lastname: Visser
authors.forename: Jukka
authors.initials: J
authors.lastname: Finne
authors.forename: Roland J
authors.initials: RJ
authors.lastname: Pieters
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D001426:Bacterial Proteins / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D008163:Luminescent Measurements; D008280:Magnetics; D010316:Particle Size; D017011:Streptococcus suis / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; D000255:Adenosine Triphosphate