title: Fluctuations in the gene expression of peripheral blood mononuclear cells between the active and inactive phases of systemic lupus erythematosus.
abstract: OBJECTIVE
  The changes in the gene expression in peripheral blood mononuclear cells (PBMC) associated with disease progression in systemic lupus erythematosus (SLE) patients with their diseases activities were examined and genes related to the pathogenesis and/or disease activities of SLE were investigated.
  
  
  METHODS
  Analyses of gene expression were performed by both DNA microarray and real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) methods.
  
  
  RESULTS
  Nine known genes showing either significantly increased or decreased expression were detected between patients with active and inactive disease phases of SLE or normal volunteers.
  
  
  CONCLUSIONS
  Among these nine genes, three genes were related to interferon (IFN) regulatory factor and four genes associated with ribosomal proteins (RPs), and two genes were associated with genetic translation factor (GTF), respectively. These three gene groups appear to contribute to the pathogenesis and/or disease progression of SLE.
authors.affiliation: Institute for Environmental and Gender Specific Medicine, Juntendo University Graduate School of Medicine, Chiba, Japan.
authors.forename: Mikiko
authors.initials: M
authors.lastname: Kawasaki
authors.forename: Maki
authors.initials: M
authors.lastname: Fujishiro
authors.forename: Ayako
authors.initials: A
authors.lastname: Yamaguchi
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Nozawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kaneko
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Takasaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Takamori
authors.forename: Hideoki
authors.initials: H
authors.lastname: Ogawa
authors.forename: Iwao
authors.initials: I
authors.lastname: Sekigawa
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D002908:Chronic Disease; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult