L-methionine-induced alterations in molecular signatures in MCF-7 and LNCaP cancer cells.
BACKGROUND
Methionine inhibits proliferation of breast and prostate cancer cells. Here, we determined the influence of L-methionine on functional molecular signatures in these cell lines.


METHODS
MCF-7 and LNCaP cells were treated with L-methionine (5 mg/ml) for 72 h. Changes in molecular signatures of these cells were examined by microarray analysis of 15,814 probes in triplicate experiments.


RESULTS
In LNCaP cells, 325 genes were up-regulated by methionine, and 517 genes down-regulated. In MCF-7 cells, 86 genes were up-regulated and 135 genes down-regulated. Ninety-eight genes were regulated in the same direction by methionine in both cells lines, and five other genes were changed in expression in opposite directions.


CONCLUSIONS
Several of the up-regulated genes encode proteins involved in cellular redox regulation, suggesting that methionine may enhance antioxidant mechanisms. Many of the down-regulated genes belong to protein kinase families that may be related to the anti-proliferative effects of methionine on breast and prostate cancer cells.
Benavides
Maximo A
MA
Department of Pathology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA, maxbenav@uic.edu
Hu
Dong
D
Baraoidan
Marie Kristine
MK
Bruno
Annette
A
Du
Pan
P
Lin
Simon
S
Yang
Wancai
W
Bland
Kirby I
KI
Grizzle
William E
WE
Bosland
Maarten C
MC
D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D008297:Male; D008715:Methionine / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D008715:Methionine
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