Neuroprotective effects of a novel peptide purified from venison protein.
A novel antioxidative peptide (APVPH I, antioxidative peptides from venison protein hydrolysates I) was purified from venison by enzymatic hydrolysis, column chromatography of DEAE-Sephacel and high performance liquid chromatography. The molecular weight of the purified peptide was found to be 9,853 Da and the amino acid sequences of the purified peptide was Met-Gln-Ile-Phe-Val-Lys-Thr-Leu-Thr-Gly. The purpose of this study was to evaluate the effects of APVPH I against H2O2-induced neuronal cells damage in PC-12 cells. Antioxidative enzyme levels in cultured neuronal cells were increased in the presence of the peptide. In addition, APVPH I inhibited productions of nitric oxide (NO), reactive oxygen species (ROS), malondialdehyde (MDA) and cell death against H2O2-induced neuronal cells damage in PC-12 cells. It was presumed to be APVPH I involved in regulating the apoptosis-related gene expression in the cell environment. Present results indicate APVPH I substantially contribute to antioxidative properties in neuronal cells.
Kim
Eun-Kyung
EK
Department of Biotechnology, Konkuk University, Chungju 380-701 Korea.
Lee
Seung-Jae
SJ
Moon
Sang-Ho
SH
Jeon
Byong-Tae
BT
Kim
Bokyung
B
Park
Tae-Kyu
TK
Han
Ji-Sook
JS
Park
Pyo-Jam
PJ
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism; D002460:Cell Line; D045304:Cytochromes c / Q000378:metabolism; D003670:Deer; D005434:Flow Cytometry; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000378:metabolism; D007150:Immunohistochemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000037:antagonists & inhibitors / Q000378:metabolism; D008460:Meat; D009124:Muscle Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism
D000975:Antioxidants; D009124:Muscle Proteins; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D008315:Malondialdehyde; D045304:Cytochromes c; D002374:Catalase; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase