title: Isolation and characterization of Psacotheasin, a novel Knottin-type antimicrobial peptide, from Psacothea hilaris.
abstract: We report the isolation and characterization of a novel knottin-type antimicrobial peptide from the yellow-spotted long-horned beetle Psacothea hilaris. A cDNA encoding a 56-mer knottin-type propeptide was identified and its predicted molecular mass and pI was 5.92 kDa and 8.28, respectively. A 34-mer mature peptide was also selected and named herein as psacotheasin. The antimicrobial activity of chemically synthesized psacotheasin against human bacterial pathogens was subsequently investigated. The results showed that psacotheasin exerted potent activities against both Gram-positive and Gram-negative bacterial strains. The present study suggests that psacotheasin can be applied to develop novel therapeutic agents.
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon 441-100, Korea.
authors.forename: Jae-Sam
authors.initials: JS
authors.lastname: Hwang
authors.forename: Juneyoung
authors.initials: J
authors.lastname: Lee
authors.forename: Bomi
authors.initials: B
authors.lastname: Hwang
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Nam
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Yun
authors.forename: Seong-Ryul
authors.initials: SR
authors.lastname: Kim
authors.forename: Dong Gun
authors.initials: DG
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D001517:Coleoptera / Q000737:chemistry*; D047168:Cystine Knot Motifs; D020869:Gene Expression Profiling; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D023181:Antimicrobial Cationic Peptides; D012333:RNA, Messenger