Development of a peptide-based immunochromatographic strip for differentiation of serotype O Foot-and-mouth disease virus-infected pigs from vaccinated pigs.
An immunochromatographic strip for discriminating Foot-and-mouth disease virus (FMDV) infected from vaccinated pigs was developed based on synthetic peptide. Five peptides designed from the amino acid sequences of nonstructural proteins (NSP) of FMDV were synthesized, and pep5 located in NSP 3B reacted strongly with serum from FMDV-infected pigs but did not react with serum samples from healthy vaccinated pigs. An immunochromatographic strip was developed by using colloidal gold labeled with pep5 as the detector. Staphylococcal protein A and rabbit against peptide-conjugated ovalbumin antibody immunoglobulin G were blotted on the nitrocellulose membrane for the test and control lines. In comparison with 2 commercial NSP enzyme-linked immunosorbent assays, the peptide-based strip showed good specificity and sensitivity. The apparent agreements of this new assay with Ceditest(R) ELISA and UBI(R) ELISA were 98.59% and 96.63%, respectively. These results indicate that the strip can be adequately used to discriminate FMDV-infected animals from vaccinated animals.
Yang
Suzhen
S
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, PR, China.
Yang
Jifei
J
Zhang
Gaiping
G
Qiao
Songlin
S
Wang
Xuannian
X
Zhao
Dong
D
Li
Xuewu
X
Deng
Ruiguang
R
Zhi
Aimin
A
You
Leiming
L
Chai
Sujun
S
Teng
Man
M
D000595:Amino Acid Sequence; D000818:Animals; D002353:Carrier State / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control / Q000635:transmission / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D015511:Goat Diseases / Q000276:immunology / Q000821:virology; D006041:Goats; D007074:Immunoglobulin G / Q000276:immunology; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000276:immunology; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000821:virology*; D014611:Vaccination / Q000379:methods / Q000662:veterinary*; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
D007074:Immunoglobulin G; D010446:Peptide Fragments; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines; D010047:Ovalbumin