title: Chip electrophoresis with mass spectrometric detection in record speed.
abstract: We demonstrate that the combination of high speed separations on chip with a fast mass spectrometer enables electrophoretic separations with full mass spectra registration within a second. This was accomplished by coupling a microfluidic glass chip with an integrated nanospray emitter to a fast time of flight mass spectrometer working at 100 Hz for data acquisition. Applying field strengths up to 5800 V cm(-1) we achieved separations of model analytes such as pharmaceuticals and peptides with subsequent acquisition of full mass spectra within one second or slightly more.
authors.affiliation: Institute of Analytical Chemistry, University of Leipzig, Linnéstr. 3, 04103, Leipzig, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Fritzsche
authors.forename: Peter
authors.initials: P
authors.lastname: Hoffmann
authors.forename: Detlev
authors.initials: D
authors.lastname: Belder
mesh_terms: D001120:Arginine / Q000032:analysis / Q000302:isolation & purification; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques; D009525:Niacin / Q000032:analysis / Q000302:isolation & purification; D013095:Spermidine / Q000032:analysis / Q000302:isolation & purification; D013997:Time Factors
chemical_list: D009525:Niacin; D001120:Arginine; D013095:Spermidine