title: Immune modulation of co-transplantation mesenchymal stem cells with islet on T and dendritic cells.
abstract: Allogeneic pancreatic islet transplantation theoretically represents a cure for type 1 diabetes. However, current immune suppressive therapies are often associated with undesired side effects. Given this problem, and the shortage of human islet donors, the majority of type 1 diabetes patients cannot currently be offered an islet transplant. However, it has been found that mesenchymal stem cells (MSCs) could exert unique immunosuppressive effects both in vitro and in vivo. Herein we transplanted allogeneic 200 islets alone or in combination with MSCs (3 x 10(6) cells) under the kidney capsules of diabetic C57LB/6 mouse. We found that the ratios of T helper type 1 (Th1) to Th2 and Tc1 to Tc2 were reduced, and the numbers of naive and memory T cells were down-regulated in peripheral blood after transplantation. In addition, the maturation, endocytosis and interleukin-12 secretion of dendritic cell (DCs)-derived bone marrow cells (BMCs) from receptor mice were suppressed. Rejection reaction was alleviated by MSCs which exerted suppressive effects through T lymphocyte subsets and DCs.
authors.affiliation: Clinical Medical Research Center, the Second Clinical Medical College (Shenzhen People's Hospital) Ji'nan University, Shenzhen, China. frli62@yahoo.com
authors.forename: F R
authors.initials: FR
authors.lastname: Li
authors.forename: X G
authors.initials: XG
authors.lastname: Wang
authors.forename: C Y
authors.initials: CY
authors.lastname: Deng
authors.forename: H
authors.initials: H
authors.lastname: Qi
authors.forename: L L
authors.initials: LL
authors.lastname: Ren
authors.forename: H X
authors.initials: HX
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use; D015703:Antigens, CD / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D039521:CD11c Antigen / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D003911:Dextrans / Q000276:immunology; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000473:pathology / Q000628:therapy*; D007136:Immunoglobulins / Q000378:metabolism; D056747:Immunomodulation / Q000276:immunology*; D018664:Interleukin-12 / Q000378:metabolism; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D007668:Kidney / Q000473:pathology; D017493:Leukocyte Common Antigens / Q000276:immunology; D018655:Lymphocyte Count; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology; D018418:Th2 Cells / Q000166:cytology / Q000276:immunology; D000097802:CD83 Antigen
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D051940:B7-2 Antigen; D001786:Blood Glucose; D039521:CD11c Antigen; D003911:Dextrans; D007136:Immunoglobulins; D018664:Interleukin-12; D017493:Leukocyte Common Antigens; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen
references.citation: Transplant Proc. 2009 Jun;41(5):1797-800
references.pmid: 19545731
references.citation: Cytotherapy. 2007;9(5):451-8
references.pmid: 17786606
references.citation: Cell Stem Cell. 2008 Feb 7;2(2):106-8
references.pmid: 18371428
references.citation: Pancreas. 2009 Mar;38(2):227-30
references.pmid: 19238026
references.citation: J Autoimmun. 2009 Mar;32(2):116-24
references.pmid: 19217258
references.citation: Transplantation. 2004 Feb 15;77(3):386-91
references.pmid: 14966412
references.citation: Blood. 2004 Mar 1;103(5):1662-8
references.pmid: 14592819
references.citation: Blood. 2005 Feb 15;105(4):1815-22
references.pmid: 15494428
references.citation: Am J Transplant. 2007 Feb;7(2):336-46
references.pmid: 17283484
references.citation: J Immunol. 2007 May 15;178(10):6028-32
references.pmid: 17475825
references.citation: Immunity. 2005 Feb;22(2):259-70
references.pmid: 15723813
references.citation: J Immunol. 2007 Sep 1;179(5):2824-31
references.pmid: 17709496
references.citation: Clin Immunol. 2008 Jun;127(3):348-58
references.pmid: 18387852
references.citation: Blood. 2003 May 1;101(9):3722-9
references.pmid: 12506037
references.citation: Int J Biochem Cell Biol. 2006;38(5-6):931-50
references.pmid: 16168703
references.citation: Br J Haematol. 2002 Mar;116(3):702-9
references.pmid: 11849236
references.citation: J Hepatobiliary Pancreat Surg. 2009;16(2):101-12
references.pmid: 19110649
references.citation: Blood. 2002 May 15;99(10):3838-43
references.pmid: 11986244
references.citation: J Exp Med. 1997 Oct 6;186(7):1119-28
references.pmid: 9314560
references.citation: J Clin Endocrinol Metab. 2005 Jun;90(6):3401-9
references.pmid: 15769977
references.citation: Transplantation. 2007 Jan 15;83(1):71-6
references.pmid: 17220794
references.citation: J Immunol. 2007 Nov 15;179(10):6547-54
references.pmid: 17982043
references.citation: Blood. 2009 Jan 1;113(1):46-57
references.pmid: 18832657
references.citation: Eur J Immunol. 2009 Sep;39(9):2325-30
references.pmid: 19701895
references.citation: Stem Cells Dev. 2007 Oct;16(5):719-31
references.pmid: 17999594
references.citation: Immunol Lett. 2008 Jan 15;115(1):50-8
references.pmid: 18022251
references.citation: Pancreas. 2009 May;38(4):447-53
references.pmid: 19258917
references.citation: J Autoimmun. 2009 Feb;32(1):33-42
references.pmid: 19062254
references.citation: Stem Cells. 2008 Oct;26(10):2531-41
references.pmid: 18635870
references.citation: J Cell Biochem. 2006 Aug 1;98(5):1076-84
references.pmid: 16619257
references.citation: Blood. 2004 Jun 15;103(12):4619-21
references.pmid: 15001472
references.citation: Blood. 2005 Mar 1;105(5):2214-9
references.pmid: 15514012