title: Beta-adrenoceptor mediated surgery-induced production of pro-inflammatory cytokines in rat microglia cells.
abstract: Immunological changes initiated by major operative injury may result in inflammatory responses in both peripheral and central nervous system, which may lead to organ dysfunction. Recent studies indicate that beta-adrenergic receptors (beta-ARs) may mediate production of pro-inflammatory cytokines in the brain. In the present study propranolol (beta-AR antagonist), but not prazosin (alpha1-AR antagonist), antagonized surgical trauma induced pro-inflammatory cytokine production in microglia cells isolated from rats. beta-AR activation in the absence of pro-inflammatory stimuli increased IL-1beta, TNF-alpha and IL-6 mRNA and protein expressions in the primary microglia cell culture. Isoproterenol (beta-AR agonist) treatment induced a time- and concentration-dependent increase of IL-1beta in cells. Both ERK1/2 and P38 MAPK inhibitor, but not PKA and JNK1/2 inhibitor abrogated isoproterenol-induced IL-1beta and IL-6 production in microglia cells. In conclusion, the results suggest that beta-ARs possess pro-inflammatory properties by modulating the functions of microglia cell.
authors.affiliation: Department of Integrative Medicine and Neurobiology, State Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai, China. jwangf@shmu.edu.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Sheng
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Xiao-Ding
authors.initials: XD
authors.lastname: Cao
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Wang
authors.forename: Gen-Cheng
authors.initials: GC
authors.lastname: Wu
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000502:physiology; D004306:Dose-Response Relationship, Immunologic; D018836:Inflammation Mediators / Q000378:metabolism* / Q000502:physiology; D008297:Male; D017628:Microglia / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D011183:Postoperative Complications / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000502:physiology*
chemical_list: D000319:Adrenergic beta-Antagonists; D016207:Cytokines; D018836:Inflammation Mediators; D011943:Receptors, Adrenergic, beta