Development of a method for the identification of S-IgA-coated bacterial composition in mouse and human feces.
Some commensal intestinal bacteria in humans and mice are coated with secretory immunoglobulin A (S-IgA). It has been suggested that the S-IgA coating of commensal bacteria does not occur at random and thus identification of S-IgA-coated bacterial genera/species should help in elucidating the interaction between S-IgA and commensal intestinal bacteria, but no method of identifying the genera/species of S-IgA-coated bacteria has been established. To identify S-IgA-coated bacterial composition, we developed a method combining immunohistochemical detection of S-IgA and subsequent 16S rRNA targeted fluorescence in situ hybridization (FISH) analysis. Human and mouse fecal S-IgA coated bacterial composition was evaluated by this newly developed method with 10 frequently-used FISH probes. Fecal S-IgA-coated bacterial composition was successfully analyzed by this method, and this suggests that Enterobacteriaceae is preferably coated with S-IgA, whereas Bacteroides/Prevotella and Lactobacillus/Enterococcus groups appear to be poorly coated with S-IgA.
Tsuruta
Takeshi
T
Laboratory of Nutritional Biochemistry, Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan. ahshoun@mail.ecc.u-tokyo.ac.jp
Inoue
Ryo
R
Iwanaga
Toshihiko
T
Hara
Hiroshi
H
Yajima
Takaji
T
D000328:Adult; D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D005243:Feces / Q000382:microbiology*; D005260:Female; D005898:Glass; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D015203:Reproducibility of Results
D007071:Immunoglobulin A, Secretory