title: Proteomic profiling of human keratinocytes undergoing UVB-induced alternative differentiation reveals TRIpartite Motif Protein 29 as a survival factor.
abstract: BACKGROUND
  Repeated exposures to UVB of human keratinocytes lacking functional p16(INK-4a) and able to differentiate induce an alternative state of differentiation rather than stress-induced premature senescence.
  
  
  RESULTS
  A 2D-DIGE proteomic profiling of this alternative state of differentiation was performed herein at various times after the exposures to UVB. Sixty-nine differentially abundant protein species were identified by mass spectrometry, many of which are involved in keratinocyte differentiation and survival. Among these protein species was TRIpartite Motif Protein 29 (TRIM29). Increased abundance of TRIM29 following UVB exposures was validated by Western blot using specific antibody and was also further analysed by immunochemistry and by RT-PCR. TRIM29 was found very abundant in keratinocytes and reconstructed epidermis. Knocking down the expression of TRIM29 by short-hairpin RNA interference decreased the viability of keratinocytes after UVB exposure. The abundance of involucrin mRNA, a marker of late differentiation, increased concomitantly. In TRIM29-knocked down reconstructed epidermis, the presence of picnotic cells revealed cell injury. Increased abundance of TRIM29 was also observed upon exposure to DNA damaging agents and PKC activation. The UVB-induced increase of TRIM29 abundance was dependent on a PKC signaling pathway, likely PKCdelta.
  
  
  CONCLUSIONS
  These findings suggest that TRIM29 allows keratinocytes to enter a protective alternative differentiation process rather than die massively after stress.
authors.affiliation: Research Unit of Cellular Biology, University of Namur (FUNDP), Namur, Belgium.
authors.forename: Véronique
authors.initials: V
authors.lastname: Bertrand-Vallery
authors.forename: Nathalie
authors.initials: N
authors.lastname: Belot
authors.forename: Marc
authors.initials: M
authors.lastname: Dieu
authors.forename: Edouard
authors.initials: E
authors.lastname: Delaive
authors.forename: Noëlle
authors.initials: N
authors.lastname: Ninane
authors.forename: Catherine
authors.initials: C
authors.lastname: Demazy
authors.forename: Martine
authors.initials: M
authors.lastname: Raes
authors.forename: Michel
authors.initials: M
authors.lastname: Salmon
authors.forename: Yves
authors.initials: Y
authors.lastname: Poumay
authors.forename: Florence
authors.initials: F
authors.lastname: Debacq-Chainiaux
authors.forename: Olivier
authors.initials: O
authors.lastname: Toussaint
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects / Q000528:radiation effects*; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects; D055785:Gene Knockdown Techniques; D020868:Gene Silencing / Q000187:drug effects; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D018832:Molecular Chaperones; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D019098:Telomerase / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism*; D014466:Ultraviolet Rays; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; C087296:TRIM29 protein, human; D014157:Transcription Factors; D011493:Protein Kinase C; D048051:p38 Mitogen-Activated Protein Kinases; C509186:TERT protein, human; D019098:Telomerase; D013755:Tetradecanoylphorbol Acetate
references.citation: Cancer Cell. 2009 Mar 3;15(3):207-19
references.pmid: 19249679
references.citation: Mol Cell Biol. 2000 Feb;20(4):1436-47
references.pmid: 10648628
references.citation: J Cell Physiol. 2002 Jul;192(1):1-15
references.pmid: 12115731
references.citation: J Dermatol Sci. 2003 Nov;33(2):105-11
references.pmid: 14581136
references.citation: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references.pmid: 6606682
references.citation: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2006 Jul;150(1):25-38
references.pmid: 16936899
references.citation: Biogerontology. 2010 Apr;11(2):167-81
references.pmid: 19554468
references.citation: J Biomed Sci. 2007 May;14(3):303-12
references.pmid: 17334833
references.citation: J Invest Dermatol. 2000 Sep;115(3):427-34
references.pmid: 10951279
references.citation: Pharmacol Ther. 1984;25(1):83-125
references.pmid: 6149569
references.citation: Ann Surg Oncol. 2007 Sep;14(9):2543-9
references.pmid: 17597343
references.citation: Mol Biol Cell. 2002 Jun;13(6):1857-70
references.pmid: 12058054
references.citation: Biol Chem. 2001 Dec;382(12):1697-705
references.pmid: 11843183
references.citation: DNA Repair (Amst). 2004 Jun 3;3(6):567-80
references.pmid: 15135725
references.citation: Br J Dermatol. 2004 Apr;150(4):658-67
references.pmid: 15099361
references.citation: Cancer Biol Ther. 2005 Sep;4(9):1018-29
references.pmid: 16251803
references.citation: J Biol Chem. 2003 Aug 1;278(31):29136-44
references.pmid: 12754261
references.citation: Cancer Res. 1999 Nov 1;59(21):5464-70
references.pmid: 10554019
references.citation: J Biol Chem. 2002 Mar 29;277(13):10775-82
references.pmid: 11788583
references.citation: Mutat Res. 2009 Mar-Jun;681(2-3):197-208
references.pmid: 18845270
references.citation: Semin Cancer Biol. 1993 Apr;4(2):93-104
references.pmid: 8513152
references.citation: Verh Dtsch Ges Pathol. 2002;86:165-75
references.pmid: 12647366
references.citation: Arch Dermatol Res. 2009 Mar;301(3):227-37
references.pmid: 18979106
references.citation: Expert Rev Proteomics. 2005 Oct;2(5):809-20
references.pmid: 16209658
references.citation: Cell Death Differ. 2005 Mar;12(3):224-32
references.pmid: 15618968
references.citation: J Cell Biol. 1995 Apr;129(1):147-56
references.pmid: 7698981
references.citation: EMBO Rep. 2004 Aug;5(8):772-6
references.pmid: 15289825
references.citation: Biochem Biophys Res Commun. 2003 Mar 28;303(1):350-6
references.pmid: 12646210
references.citation: J Biol Chem. 2002 Aug 30;277(35):31753-60
references.pmid: 12080077
references.citation: Cell Mol Life Sci. 2004 Oct;61(19-20):2614-23
references.pmid: 15526166
references.citation: J Biol Chem. 1991 Aug 25;266(24):15771-81
references.pmid: 1874734
references.citation: Exp Dermatol. 2003 Dec;12(6):811-24
references.pmid: 14714562
references.citation: J Cancer Res Clin Oncol. 2007 Nov;133(11):793-808
references.pmid: 17661083
references.citation: J Cell Biol. 2006 Jul 3;174(1):115-25
references.pmid: 16818723
references.citation: Physiol Rev. 1997 Apr;77(2):397-424
references.pmid: 9114819
references.citation: J Biol Chem. 2002 May 10;277(19):16547-52
references.pmid: 11864976
references.citation: J Cell Biol. 2001 Aug 20;154(4):775-84
references.pmid: 11514591
references.citation: Toxicology. 2003 Jul 15;189(1-2):21-39
references.pmid: 12821280
references.citation: J Invest Dermatol. 2005 Nov;125(5):1026-31
references.pmid: 16297205
references.citation: Clin Exp Dermatol. 2001 Oct;26(7):573-7
references.pmid: 11696060
references.citation: J Biol Chem. 2002 Oct 11;277(41):38540-9
references.pmid: 12140282
references.citation: Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7824-8
references.pmid: 7644499
references.citation: Int J Radiat Biol. 1989 Nov;56(5):667-75
references.pmid: 2573662
references.citation: Ageing Res Rev. 2002 Sep;1(4):705-20
references.pmid: 12208239
references.citation: Cell Death Differ. 2003 Jun;10(6):754-6
references.pmid: 12761584
references.citation: Int J Cancer. 1996 Jun 11;66(6):772-8
references.pmid: 8647648
references.citation: FASEB J. 2001 Nov;15(13):2533-5
references.pmid: 11641260
references.citation: J Invest Dermatol. 2003 Jul;121(1):51-64
references.pmid: 12839563
references.citation: Br J Dermatol. 2002 May;146(5):777-85
references.pmid: 12000373
references.citation: Genes Dev. 2001 Sep 1;15(17):2177-96
references.pmid: 11544175
references.citation: Cancer Res. 2004 May 1;64(9):3009-13
references.pmid: 15126335
references.citation: Proteomics. 2001 May;1(5):699-704
references.pmid: 11678039
references.citation: Mol Biol Cell. 2008 Apr;19(4):1346-53
references.pmid: 18216278
references.citation: Exp Cell Res. 1961 Dec;25:585-621
references.pmid: 13905658
references.citation: J Drugs Dermatol. 2003 Aug;2(4):385-91
references.pmid: 12884460
references.citation: Dev Biol. 1997 Mar 15;183(2):123-38
references.pmid: 9126289
references.citation: Int J Biochem Cell Biol. 2004 Jul;36(7):1141-6
references.pmid: 15109560