title: Detection of polyomavirus major capsid antigen (VP-1) in human pilomatricomas.
abstract: The family Polyomaviridae is composed of small, non-enveloped, double-stranded DNA viruses widely used to study cell transformation in vitro and tumor induction in vivo. The development of pilomatricomas in mice experimentally infected with polyomavirus led us to detect the viral major capsid protein VP-1 in human pilomatricomas. This tumor, even uncommon, is one of the most frequent benign hair follicle tumors in humans and is composed of proliferating matrix cells that undergo keratinization, and form cystic neoplasms. The detection of VP-1 was performed using the peroxidase-antiperoxidase technique in paraffin-embedded slides with a specific primary serum. Adjacent slides treated with normal rabbit serum as a primary were employed as internal control. Positive and negative controls were also employed as well as slides of lesions caused by human papillomavirus to rule out any unspecific cross-reactivity. In 4 out of 10 cases polyomavirus VP-1 was clearly detected in nuclei of human pilomatricomas proliferating cells, in a patchy pattern of distribution. The controls confirmed the specificity of the immunocytochemical procedure. These results could indicate either an eventual infection of the virus in already developed tumors or alternatively, a direct involvement of polyomavirus in the pathogenesis of some pilomatricomas. The recent discovery of a new human polyomavirus associated with Merkel cell carcinomas has been a strong contribution to better understand the pathogenesis of some human uncommon skin cancers. Hopefully the results reported in this work will encourage further research on the role of polyomavirus in other human skin neoplasms.
authors.affiliation: Laboratorio de Patología Experimental, Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina. patoexpe@fmed.uba.ar
authors.forename: Norberto A
authors.initials: NA
authors.lastname: Sanjuán
authors.forename: Silvina
authors.initials: S
authors.lastname: Símula
authors.forename: José
authors.initials: J
authors.lastname: Casas
authors.forename: Alberto
authors.initials: A
authors.lastname: Woscoff
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D036022:Capsid Proteins / Q000032:analysis*; D006201:Hair Diseases / Q000473:pathology / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D016612:Paraffin Embedding; D018296:Pilomatrixoma / Q000473:pathology / Q000821:virology*; D027601:Polyomavirus Infections / Q000473:pathology*; D012878:Skin Neoplasms / Q000473:pathology / Q000821:virology*; D014412:Tumor Virus Infections / Q000473:pathology / Q000821:virology*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C062481:VP1 protein, polyomavirus