title: Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone release functionality.
abstract: Electrospun structures were proposed as scaffolds owing to their morphological and structural similarities with the extracellular matrix found in many native tissues. These fibrous structures were also proposed as drug release systems by exploiting the direct dependence of the release rate of a drug on the surface area. An osteogenic differentiation factor, dexamethasone (DEX), was incorporated into electrospun polycaprolactone (PCL) nanofibers at different concentrations (5, 10, 15 and 20 wt.% polymer), in a single-step process. The DEX incorporated into the polymeric carrier is in amorphous state, as determined by DSC, and does not influence the typical nanofibers morphology. In vitro drug release studies demonstrated that the dexamethasone release was sustained over a period of 15 days. The bioactivity of the released dexamethasone was assessed by cultivating human bone marrow mesenchymal stem cells (hBMSCs) on 15 wt.% DEX-loaded PCL NFMs, under dexamethasone-absent osteogenic differentiation medium formulation. An increased concentration of alkaline phosphatase and deposition of a mineralized matrix was observed. Phenotypic and genotypic expression of osteoblastic-specific markers corroborates the osteogenic activity of the loaded growth/differentiation factor. Overall data suggests that the electrospun biodegradable nanofibers can be used as carriers for the sustained release of growth/differentiation factors relevant for bone tissue engineering strategies.
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho; Avepark, Zona Industrial da Gandra, S. Claúdio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal. amartins@dep.uminho.pt
authors.forename: Albino
authors.initials: A
authors.lastname: Martins
authors.forename: Ana Rita C
authors.initials: AR
authors.lastname: Duarte
authors.forename: Susana
authors.initials: S
authors.lastname: Faria
authors.forename: Alexandra P
authors.initials: AP
authors.lastname: Marques
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
authors.forename: Nuno M
authors.initials: NM
authors.lastname: Neves
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D002470:Cell Survival; D002478:Cells, Cultured; D003907:Dexamethasone / Q000008:administration & dosage* / Q000494:pharmacology; D055664:Electrochemical Techniques; D005938:Glucocorticoids / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005938:Glucocorticoids; D011091:Polyesters; C016240:polycaprolactone; D003907:Dexamethasone