title: Production, physiochemical and antimicrobial properties of fungal chitosan from Rhizomucor miehei and Mucor racemosus.
abstract: Chitosan was isolated and purified from the mycelia of Rhizomucor miehei and Mucor racemosus. To characterize the extracted materials, their FTIR spectra were compared with that of shrimp chitosan. Final degree of deacetylation which determined by (1)H NMR was obtained as 98.6% for chitosan from R. miehei (named as C1) and 97.1% for chitosan from M. racemosus (named as C2), respectively. To investigate the antimicrobial properties of the isolated fungal chitosans, minimum inhibitory concentration (MIC) values were performed against twelve strains of bacteria and fungi. Data obtained generally showed that the antibacterial and antifungal activities of the purified fungal chitosans were more effective against Escherichia coli, Pseudomonas aeroginosa, Candida albicans and Candida glabrata in comparison to the shrimp chitosan. Taken together, the results suggest that the use of the fungal chitosan could be of interest as a suitable alternative source to shrimp chitosan.
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran. farzaneh.tajdini@kiau.ac.ir
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Tajdini
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Amini
authors.forename: Nastaran
authors.initials: N
authors.lastname: Nafissi-Varcheh
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Faramarzi
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D055598:Chemical Phenomena; D048271:Chitosan / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D009089:Mucor / Q000378:metabolism*; D020103:Rhizomucor / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000890:Anti-Infective Agents; D048271:Chitosan