3D shortcuts to gene regulation.
Recent technologies have allowed high-resolution genome-wide binding profiles of numerous transcription factors and other proteins. A widespread observation has emerged from studies in diverse mammalian systems: most binding events are located at great distances from gene promoters. It is becoming apparent that the traditional one-dimensional view of gene regulation via the proximal cis regulatory elements is over-simplified. True proximity and functional relevance can be revealed by studying the three-dimensional structure of the genome packaged inside the nucleus. Thus the spatial architecture of the genome has attracted a lot of interest and has intensified its significance in modern cell biology. Here we discuss current methods, concepts, and controversies in this rapidly evolving field.
Hakim
Ofir
O
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Sung
Myong-Hee
MH
Hager
Gordon L
GL
D000818:Animals; D002875:Chromosomes / Q000378:metabolism; D005786:Gene Expression Regulation; D016678:Genome / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009687:Nuclear Proteins / Q000378:metabolism; D009690:Nucleic Acid Conformation
D009687:Nuclear Proteins
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