Inhibitor of DNA binding 4 (ID4) regulation of adipocyte differentiation and adipose tissue formation in mice.
Inhibitor of DNA binding 4 (ID4) is a helix-loop-helix protein that heterodimerizes with basic helix-loop-helix transcription factors inhibiting their function. ID4 expression is important for adipogenic differentiation of the 3T3-L1 cell line, and inhibition of ID4 is associated with a concomitant decrease in CCAAT/enhancer-binding protein alpha and peroxisome proliferator-activated receptor gamma mRNA and protein expression. Mice with a homozygous deletion of Id4 (Id4(-/-)) have reduced body fat and gain much less weight compared with wild-type littermates when placed on diets with high fat content. Mouse embryonic fibroblasts (MEFs) isolated from Id4(-/-) mice have reduced adipogenic potential when compared with wild-type MEFs. In agreement with changes in morphological differentiation, the levels of CCAAT/enhancer-binding protein alpha and peroxisome proliferator-activated receptor gamma were also reduced in MEFs from Id4(-/-) mice. Our results demonstrate the importance of ID4 in adipocyte differentiation and the implications of this regulation for adipose tissue formation.
Murad
Joana M
JM
Norris Cotton Cancer Center and Department of Pediatrics, Dartmouth Medical School, Hanover, NH 03755, USA.
Place
Chelsea S
CS
Ran
Cong
C
Hekmatyar
Shahryar K N
SK
Watson
Nathan P
NP
Kauppinen
Risto A
RA
Israel
Mark A
MA
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D002454:Cell Differentiation; D019281:Dimerization; D005347:Fibroblasts / Q000378:metabolism; D017353:Gene Deletion; D005838:Genotype; D006720:Homozygote; D051796:Inhibitor of Differentiation Proteins / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological
C086479:Idb4 protein, mouse; D051796:Inhibitor of Differentiation Proteins
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