Direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of Aspergillus oryzae A-4 in solid-state fermentation.
Direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of Aspergillus oryzae A-4 in solid-state fermentation (SSF) was investigated. In submerged fermentation, A. oryzae A-4 accumulated lipid to 15-18.15% of biomass when pure cellulose was utilized as the sole substrate. In SSF of the wheat straw and bran mixture, A. oryzae A-4 yielded lipid of 36.6mg/g dry substrate (gds), and a cellulase activity of 1.82 FPU/gds with 25.25% of holocellulose utilization in the substrates were detected on the 6th day. The lipid yield reached 62.87 mg/gds in SSF on the 6th day under the optimized conditions from Plackett-Burman design (PBD). Cellulase secretion of A. oryzae A-4 was found to influence the lipid yield. Dilute acid pretreatment of the straw and addition of some agro-industrial wastes to the straw could enhance lipid production of A. oryzae A-4.
Lin
Hui
H
Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, China.
Cheng
Wan
W
Ding
Hai-tao
HT
Chen
Xue-jiao
XJ
Zhou
Qi-fa
QF
Zhao
Yu-hua
YH
D001236:Aspergillus oryzae / Q000187:drug effects / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation / Q000187:drug effects; D008055:Lipids / Q000096:biosynthesis*; D008954:Models, Biological; D013379:Substrate Specificity / Q000187:drug effects; D013997:Time Factors; D014908:Triticum / Q000187:drug effects / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis*
D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; D014866:Waste Products; D002482:Cellulose; D002480:Cellulase