Electroacupuncture pretreatment ameliorates hypergravity-induced impairment of learning and memory and apoptosis of hippocampal neurons in rats.
High-sustained positive acceleration (+Gz) exposures might lead to impairment in cognitive function. Our previous studies have shown that electroacupuncture (EA) pretreatment can attenuate transient focal cerebral ischemic injury in the rats. In this study we aimed to investigate whether EA pretreatment could ameliorate the impairment of learning and memory induced by a sustained +Gz exposure. Using the centrifuge model, rats of experimental groups were exposed to +10 Gz for 5 min. Morris water maze was used for assessing the cognitive ability, and the apoptotic hippocampal CA1 pyramidal neuronal cells were evaluated by caspase-3 activity and TUNEL staining. Our results showed that +Gz exposure significantly caused pyramidal neuronal damage, increased neuronal apoptosis and caspase-3 activity in hippocampal CA1 region, as well as resulted in an impairment of spatial learning and memory, as compared to the sham group animals. Furthermore, the EA pretreatment significantly attenuated the neuronal apoptosis, preserved neuronal morphology and inhibited the caspase-3 activity in hippocampal CA1 region resulted from +Gz exposure. The EA pretreatment also ameliorated the learning and memory function in rats exposed to +Gz. These findings indicate that EA pretreatment provides a novel method to prevent the cognitive damage caused by +Gz, which could significantly protect neuronal damage and impairment of learning and memory.
Feng
Shufang
S
Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, China.
Wang
Qiang
Q
Wang
Huaning
H
Peng
Ye
Y
Wang
Lei
L
Lu
Yan
Y
Shi
Tianyao
T
Xiong
Lize
L
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D056547:CA1 Region, Hippocampal / Q000473:pathology / Q000503:physiopathology; D053148:Caspase 3 / Q000378:metabolism; D002498:Centrifugation; D015671:Electroacupuncture / Q000379:methods*; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology*; D018471:Hypergravity / Q000009:adverse effects*; D007859:Learning Disabilities / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D018782:Maze Learning / Q000502:physiology; D008569:Memory Disorders / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D009474:Neurons / Q000473:pathology / Q000502:physiology; D009483:Neuropsychological Tests; D017966:Pyramidal Cells / Q000473:pathology / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
C505410:Casp3 protein, rat; D053148:Caspase 3