title: The NucleoSpin® DNA Clean-up XS kit for the concentration and purification of genomic DNA extracts: an alternative to microdialysis filtration.
abstract: Traditionally, DNA extracts from biological evidence items have been concentrated and rinsed using microdialysis filtration units, including the Centricon(®) and Microcon(®) centrifugal filter devices. As an alternative to microdialysis filtration, we present an optimized method for using NucleoSpin(®) XS silica columns to concentrate and clean-up aqueous extracts from the organic extraction of DNA from biological samples. The method can be used with standard organic extraction and dithiothreitol (DTT)-based differential extraction methods with no modifications to these methods prior to the concentration and clean-up step. Extracts from laboratory-prepared bloodstains, saliva and semen stains have been successfully amplified with both qPCR and STR assays. Finally, the total time to process a set of samples with the NucleoSpin(®) XS column is approximately 30 min vs. approximately 1.5h with the Centricon(®) YM-100 filter device.
authors.affiliation: California Department of Justice Jan Bashinski DNA Laboratory, 1001 W. Cutting Blvd., Suite 110, Richmond, CA 94804, USA. Bill.Hudlow@doj.ca.gov
authors.forename: William R
authors.initials: WR
authors.lastname: Hudlow
authors.forename: Robert
authors.initials: R
authors.lastname: Krieger
authors.forename: Markus
authors.initials: M
authors.lastname: Meusel
authors.forename: Joshua C
authors.initials: JC
authors.lastname: Sehhat
authors.forename: Mark D
authors.initials: MD
authors.lastname: Timken
authors.forename: Martin R
authors.initials: MR
authors.lastname: Buoncristiani
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D005374:Filtration / Q000379:methods*; D006801:Humans; D017551:Microdialysis / Q000379:methods*; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA