title: One pot synthesis of new hybrid versatile nanocarrier exhibiting efficient stability in biological environment for use in photodynamic therapy.
abstract: A new versatile hybrid nanocarrier has been designed using a "soft chemistry" synthesis, to efficiently encapsulate a photosensitizer - the protoporphyrin IX (Pp IX) - while preserving its activity intact in biological environment for advantageous use in photodynamic therapy (PDT). The synthesized Pp IX silica-based nanocarriers show to be spherical in shape and highly monodisperse with size extending from 10 nm up to 200 nm according to the synthesis procedure. Upon laser irradiation, the entrapped Pp IX shows to efficiently deliver reactive oxygen species (ROS) which are responsible for damaging tumor tissues. The ability of Pp IX silica-based nanocarriers to induce tumor cell death has been tested successfully in vitro. The stability of the Pp IX silica-based nanocarriers has been followed by UV-vis absorption and fluorescence emission in aqueous media and in 100% mouse serum media. The flexibility of the nanocarrier silica core has been examined as the key parameter to tune the Pp IX stability in biological environment. Indeed, an additional biocompatible inorganic surface coating performed on the Pp IX silica-based nanocarriers to produce an optimized bilayer coating demonstrates to significantly enhance the Pp IX stabilization in biological environments. Such versatile hybrid nanocarriers open new perspectives for PDT.
authors.affiliation: Nanobiotix SA, F-75012 Paris, France.
authors.forename: Edouard
authors.initials: E
authors.lastname: Thienot
authors.forename: Matthieu
authors.initials: M
authors.lastname: Germain
authors.forename: Kelthoum
authors.initials: K
authors.lastname: Piejos
authors.forename: Virginie
authors.initials: V
authors.lastname: Simon
authors.forename: Audrey
authors.initials: A
authors.lastname: Darmon
authors.forename: Julie
authors.initials: J
authors.lastname: Marill
authors.forename: Elsa
authors.initials: E
authors.lastname: Borghi
authors.forename: Laurent
authors.initials: L
authors.lastname: Levy
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Hochepied
authors.forename: Agnès
authors.initials: A
authors.lastname: Pottier
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000737:chemistry; D011524:Protoporphyrins / Q000008:administration & dosage* / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004337:Drug Carriers; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D017382:Reactive Oxygen Species; D012822:Silicon Dioxide; C028025:protoporphyrin IX