The SNF2H chromatin remodeling enzyme has opposing effects on cytokine gene expression.
Cytokine gene expression is a key control point in the function of the immune system. Cytokine gene regulation is linked to changes in chromatin structure; however, little is known about the remodeling enzymes mediating these changes. Here we investigated the role of the ATP-dependent chromatin remodeling enzyme SNF2H in mouse T cells; to date, SNF2H has not been investigated in T cells. We found that SNF2H repressed expression of IL-2 and other cytokines in activated cells. By contrast, SNF2H activated expression of IL-3. The ISWI components SNF2H and ACF1 bound to the tested loci, suggesting the regulation was direct. SNF2H decreased accessibility at some binding sites within the IL2 locus, and increased accessibility within some IL3 binding sites. The changes in gene expression positively correlated with accessibility changes, suggesting a simple model that accessibility enables transcription. We also found that loss of the ISWI ATPase SNF2H reduced binding to target genes and protein expression of ACF1, a binding partner for SNF2H, suggesting complex formation stabilized ACF1. Together, these findings reveal a direct role for SNF2H in both repression and activation of cytokine genes.
Precht
Patricia
P
Laboratory of Cellular and Molecular Biology, National Institute on Aging, NIH, Baltimore, MD 21224, USA.
Wurster
Andrea L
AL
Pazin
Michael J
MJ
D000251:Adenosine Triphosphatases / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology*; D016207:Cytokines / Q000235:genetics*; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D014157:Transcription Factors / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone; D016207:Cytokines; D014157:Transcription Factors; D000251:Adenosine Triphosphatases; C498725:Smarca5 protein, mouse
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