title: Characterization of the gene expression profile of human bocavirus.
abstract: We have generated a quantitative transcription profile of human bocavirus type 1 (HBoV1) by transfecting a nearly full-length clone in human lung epithelial A549 cells as well as in a replication competent system in 293 cells. The overall transcription profile of HBoV1 is similar to that of two other members of genus Bocavirus, minute virus of canines and bovine parvovirus 1. In particular, a spliced NS1-transcript that was not recognized previously expressed the large non-structural protein NS1 at approximately 100kDa; and the NP1-encoding transcripts were expressed abundantly. In addition, the protein expression profile of human bocavirus type 2 (HBoV2) was examined in parallel by transfection of a nearly full-length clone in A549 cells, which is similar to that of HBoV1. Moreover, our results showed that, unlike human parvovirus B19 infection, expression of the HBoV1 proteins only does not induce cell cycle arrest and apoptosis of A549 cells.
authors.affiliation: Department of Microbiology, University of Kansas Medical Center, Kansas City, KS, USA.
authors.forename: Aaron Yun
authors.initials: AY
authors.lastname: Chen
authors.forename: Fang
authors.initials: F
authors.lastname: Cheng
authors.forename: Sai
authors.initials: S
authors.lastname: Lou
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Zhengwen
authors.initials: Z
authors.lastname: Liu
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
authors.forename: David
authors.initials: D
authors.lastname: Pintel
authors.forename: Jianming
authors.initials: J
authors.lastname: Qiu
mesh_terms: D052660:Bocavirus / Q000502:physiology; D002460:Cell Line; D020869:Gene Expression Profiling; D056661:Human bocavirus / Q000502:physiology*; D006801:Humans; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral
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