Immobilization of cross-linked lipase aggregates onto magnetic beads for enzymatic degradation of polycaprolactone.
Candida rugosa lipase was immobilized on amino-functionalized magnetic supports via cross-linked enzyme aggregates (CLEA) and used to enhance the enzymatic degradation of polycaprolactone (PCL). The maximum amounts of lipase immobilized on the magnetic beads using glutaraldehyde as a coupling agent were determined to be 33.7 mg/g of beads with an 81% recovery of activity after immobilization. Compared to the free enzyme, the immobilized lipase showed the optimum pH at 1 unit higher (pH 8.0) and also retained its enzymatic activity at higher temperatures. There was 62.9% retention of lipase activity after 30 consecutive reuses, indicating its stability and reusability in aqueous media. Moreover, the immobilized lipase maintained more than 80% of its initial activity during 30 days storage period, while the free lipase lost all under same condition. In addition, the immobilized lipase showed a more than 6-fold increase in biodegradability over the free lipase when the immobilized lipase was used to degrade PCL in a batch system. Higher thermal and storage stability, as well as good durability after repeated use of the immobilized lipase CLEA, highlights its potential applicability as large scale continuous systems for the enzymatic degradation of PCL.
Kim
Mina
M
Department of Microbiology, Chungbuk National University Sungbong-Ro, Heungduk-Gu, Cheongju, South Korea.
Park
Jae-Min
JM
Um
Hyun-Ju
HJ
Lee
Dong-Hun
DH
Lee
Kyu-Ho
KH
Kobayashi
Fumihisa
F
Iwasaka
Yasunobu
Y
Hong
Chun-Sang
CS
Min
Jiho
J
Kim
Yang-Hoon
YH
D001711:Biotransformation; D002175:Candida / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005976:Glutaral / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008280:Magnetics; D008863:Microspheres; D011091:Polyesters / Q000378:metabolism*; D013696:Temperature
D004800:Enzymes, Immobilized; D011091:Polyesters; C016240:polycaprolactone; D008049:Lipase; D005976:Glutaral