title: spa typing and enterotoxin gene profile of Staphylococcus aureus isolated from bovine raw milk in Korea.
abstract: Staphylococcus aureus is a major etiological pathogen of bovine mastitis, which triggers significant economic losses in dairy herds worldwide. In this study, S. aureus strains isolated from the milk of cows suffering from mastitis in Korea were investigated by spa typing and staphylococcal enterotoxin (SE) gene profiling. Forty-four S. aureus strains were isolated from 26 farms in five provinces. All isolates grouped into five clusters and two singletons based on 14 spa types. Cluster 1 and 2 isolates comprised 38.6% and 36.4% of total isolates, respectively, which were distributed in more than four provinces. SE and SE-like toxin genes were detected in 34 (77.3%) isolates and the most frequently detected SE gene profile was seg, sei, selm, seln, and selo genes (16 isolates, 36.3%), which was comparable to one of the genomic islands, Type I nuSabeta. This is a first report of spa types and the prevalence of the recently described SE and SE-like toxin genes among S. aureus isolates from bovine raw milk in Korea. Two predominant spa groups were distributed widely and recently described SE and SE-like toxin genes were detected frequently.
authors.affiliation: Department of Microbiology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Hwang
authors.forename: Young Kyung
authors.initials: YK
authors.lastname: Park
authors.forename: Hye Cheong
authors.initials: HC
authors.lastname: Koo
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Park
mesh_terms: D000818:Animals; D002417:Cattle; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004768:Enterotoxins / Q000737:chemistry / Q000235:genetics*; D005260:Female; D005838:Genotype; D008414:Mastitis, Bovine / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology*; D018895:Microsatellite Repeats; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D056910:Republic of Korea / Q000453:epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D004768:Enterotoxins
references.citation: Vet Microbiol. 2009 May 28;137(1-2):202-6
references.pmid: 19195799
references.citation: Foodborne Pathog Dis. 2006 Fall;3(3):274-83
references.pmid: 16972776
references.citation: Mol Microbiol. 2001 Jul;41(2):365-77
references.pmid: 11489124
references.citation: Lancet. 2002 May 25;359(9320):1819-27
references.pmid: 12044378
references.citation: J Clin Microbiol. 1992 Jul;30(7):1654-60
references.pmid: 1629319
references.citation: Lancet. 2001 Apr 21;357(9264):1225-40
references.pmid: 11418146
references.citation: Infect Immun. 2006 Aug;74(8):4724-34
references.pmid: 16861660
references.citation: J Clin Microbiol. 1995 Feb;33(2):376-80
references.pmid: 7714195
references.citation: J Clin Microbiol. 1993 Mar;31(3):706-7
references.pmid: 8458968
references.citation: J Nat Toxins. 1998 Oct;7(3):193-213
references.pmid: 9783259
references.citation: Vet Microbiol. 1999 Sep 15;69(3):217-24
references.pmid: 10512046
references.citation: J Med Microbiol. 2008 Sep;57(Pt 9):1106-1112
references.pmid: 18719180
references.citation: Appl Environ Microbiol. 2007 Jun;73(12):3845-9
references.pmid: 17449696
references.citation: J Immunol. 2001 Jan 1;166(1):669-77
references.pmid: 11123352
references.citation: J Clin Microbiol. 2003 Dec;41(12):5442-8
references.pmid: 14662923
references.citation: Infect Immun. 2003 Oct;71(10):6088-94
references.pmid: 14500536
references.citation: J Appl Microbiol. 2006 Oct;101(4):864-71
references.pmid: 16968298
references.citation: Int J Food Microbiol. 2007 Jun 10;117(1):99-105
references.pmid: 17439826
references.citation: Eur J Clin Microbiol Infect Dis. 1996 Jan;15(1):60-4
references.pmid: 8641305
references.citation: J Clin Microbiol. 2000 Mar;38(3):1008-15
references.pmid: 10698988
references.citation: FEMS Microbiol Lett. 1998 Nov 15;168(2):227-33
references.pmid: 9835033
references.citation: Vet Microbiol. 2005 Feb 25;105(3-4):301-5
references.pmid: 15708828
references.citation: J Biol Chem. 2002 Apr 12;277(15):13138-47
references.pmid: 11821418
references.citation: Infect Immun. 1997 Oct;65(10):4048-54
references.pmid: 9317006
references.citation: J Dairy Sci. 2007 Apr;90(4):1716-24
references.pmid: 17369211
references.citation: J Clin Microbiol. 2006 Jul;44(7):2533-40
references.pmid: 16825376
references.citation: J Bacteriol. 2005 Apr;187(7):2426-38
references.pmid: 15774886
references.citation: J Appl Microbiol. 2000 Jun;88(6):1028-37
references.pmid: 10849179
references.citation: Vet Microbiol. 2001 Feb 26;78(4):373-82
references.pmid: 11182503
references.citation: Epidemiol Infect. 1997 Oct;119(2):261-9
references.pmid: 9363026
references.citation: J Korean Med Sci. 2009 Aug;24(4):585-91
references.pmid: 19654937
references.citation: J Clin Microbiol. 2004 Feb;42(2):792-9
references.pmid: 14766855
references.citation: J Appl Bacteriol. 1992 Jun;72(6):486-9
references.pmid: 1644704
references.citation: J Clin Microbiol. 1999 Nov;37(11):3556-63
references.pmid: 10523551
references.citation: Int J Food Microbiol. 2000 Oct 1;61(1):1-10
references.pmid: 11028954
references.citation: J Dairy Sci. 1992 Feb;75(2):596-605
references.pmid: 1560148
references.citation: J Dairy Sci. 2007 Dec;90(12):5405-14
references.pmid: 18024731
references.citation: FEMS Microbiol Lett. 2005 May 15;246(2):191-8
references.pmid: 15899405
references.citation: Clin Microbiol Rev. 2000 Jan;13(1):16-34, table of contents
references.pmid: 10627489
references.citation: Infect Immun. 2005 Sep;73(9):5540-6
references.pmid: 16113270
references.citation: J Clin Microbiol. 2008 Aug;46(8):2805-8
references.pmid: 18524961
references.citation: Clin Microbiol Rev. 2004 Jan;17(1):14-56
references.pmid: 14726454
references.citation: Appl Environ Microbiol. 1995 Apr;61(4):1438-43
references.pmid: 7747964