title: [Effect of fipronil on apoptosis rate and Bcl-2 of PC12 cells].
abstract: OBJECTIVE
  To study the apoptosis and mechanism of Fipronil on PC12 Cells.
  
  
  METHODS
  The effect of fipronil on the apoptosis and necrosis of PC12 cells of 3.13 x 10(-6), 1.25 x 10(-5) and 5.00 x 10(-5) mol/L three dose groups after 24 h treatment was detected by morphology and the apoptosis rate was detected by flow cytometer (FCM). The expression of Bcl-2 protein in PC12 cells of 3.13 x 10(-6), 1.25 x 10(-5) and 5.00 x 10(-5) mol/L three dose groups after 24 h treatment was measured by immunofluorescence.
  
  
  RESULTS
  The number of apoptotic cells of 3.13 x 10(-6) mol/L group was more than the control group examined by fluorescence microscope, and the number of dead cells of 5.00 x 10(-5) mol/L group was more than the control group. The apoptotic rates of PC12 cells was higher in the 3.13 x 10(-6) mol/L group than the control group measured by FCM, and the dead rates of PC12 cells was higher in the 5.00 x 10(-5) mol/L group than the control group (P < 0.05). Immunofluorescence cytochemistry experiment demonstrated that the level of Bcl-2 expression was significantly lower in the 3.13 x 10(-6) mol/L group than the control group.
  
  
  CONCLUSIONS
  At low dosage, fipronil increases the apoptotic rates of PC12 cells possibly by decreasing the expression of Bcl-2 protein while at high dosage, fipronil only increases the amount of necrotic cells.
authors.affiliation: Department of Health Toxicology and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Xiong-Fei
authors.initials: XF
authors.lastname: Hu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Wan
authors.initials: W
authors.lastname: Zhuang
authors.forename: Long
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D016716:PC12 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011720:Pyrazoles / Q000633:toxicity*; D051381:Rats
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D011720:Pyrazoles; C082360:fipronil