title: Optimal determination of steric mass action model parameters for beta-lactoglobulin using static batch experiments.
abstract: In this work, parameters of the steric mass-formalism SMA are optimally ascertained for a reliable determination of the adsorption isotherms of beta-lactoglobulin A and B under non-isocratic conditions. For this purpose, static batch experiments are used in contrast to the protocols based on different experimental steps, which use a chromatographic column. It is shown that parameters can already be determined for a small number of experiments by using a systematic procedure based on optimal model-based experimental design and an efficient NLP-solver. The in different works observed anti-Langmuir shape of the isotherm for small concentrations of beta-lactoglobulin A was corroborated. Moreover, we also found indications for a porosity variation with changing protein concentrations.
authors.affiliation: Berlin Institute of Technology, Sekr. KWT 9, Strasse des 17. Juni 135, D-10623 Berlin, Germany. tilman.barz@tu-berlin.de
authors.forename: Tilman
authors.initials: T
authors.lastname: Barz
authors.forename: Verena
authors.initials: V
authors.lastname: Löffler
authors.forename: Harvey
authors.initials: H
authors.lastname: Arellano-Garcia
authors.forename: Günter
authors.initials: G
authors.lastname: Wozny
mesh_terms: D000327:Adsorption; D002852:Chromatography, Ion Exchange / Q000379:methods*; D007782:Lactoglobulins / Q000737:chemistry*; D008962:Models, Theoretical; D055672:Static Electricity; D013237:Stereoisomerism; D013816:Thermodynamics
chemical_list: D007782:Lactoglobulins