Decolorization and biodegradation of reactive blue 220 textile dye by Lentinus crinitus extracellular extract.
Studies were carried on the decolorization of the textile dye reactive blue 220 (RB220) by a novel isolate of Lentinus crinitus fungi. The optimal conditions for the production of destaining activity were obtained in media containing intermediate concentrations of ammonium oxalate and glucose (10 g L(-1)) as nitrogen and carbon sources, respectively, at 28 degrees C and pH 5.5. Maximum decolorization efficiency against RB220 achieved in this study was around 95%. Ultra-violet and visible (UV-vis) spectrophotometric analyses, before and after decolorization, suggest that decolorization was due to biodegradation. This effect was associated with a putative low molecular weight laccase (41 kDa) displaying good tolerance to a wide range of pH values, salt concentrations and temperatures, suggesting a potential role for this organism in the remediation of real dye containing effluents.
Niebisch
Carolina Heyse
CH
Departamento de Bioquímica, Universidade Federal do Paraná, Setor Ciências Biológicas, Centro Politécnico, Caixa Postal, CEP 81531990, Curitiba, Paraná, Brazil.
Malinowski
Alexandre Knoll
AK
Schadeck
Ruth
R
Mitchell
David A
DA
Kava-Cordeiro
Vanessa
V
Paba
Jaime
J
D001673:Biodegradation, Environmental; D002244:Carbon; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D003300:Copper / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D020074:Lentinula / Q000378:metabolism*; D009584:Nitrogen; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013784:Textiles
D004396:Coloring Agents; D002244:Carbon; D003300:Copper; D009584:Nitrogen