title: Trichoderma harzianum IOC-4038: A promising strain for the production of a cellulolytic complex with significant β-glucosidase activity from sugarcane bagasse cellulignin.
abstract: Sugarcane bagasse is an agroindustrial residue generated in large amounts in Brazil. This biomass can be used for the production of cellulases, aiming at their use in second-generation processes for bioethanol production. Therefore, this work reports the ability of a fungal strain, Trichoderma harzianum IOC-4038, to produce cellulases on a novel material, xylan free and cellulose rich, generated from sugarcane bagasse, named partially delignified cellulignin. The extract produced by T. harzianum under submerged conditions reached 745, 97, and 559 U L(-1) of β-glucosidase, FPase, and endoglucanase activities, respectively. The partial characterization of this enzyme complex indicated, using a dual analysis, that the optimal pH values for the biocatalysis ranged from 4.9 to 5.2 and optimal temperatures were between 47 and 54 °C, depending on the activity studied. Thermal stability analyses revealed no significant decrease in activity at 37 °C during 23 h of incubation. When compared to model strains, Aspergillus niger ATCC-16404 and Trichoderma reesei RutC30, T. harzianum fermentation was faster and its extract showed a better balanced enzyme complex, with adequate characteristics for its application in simultaneous saccharification and fermentation processes.
authors.affiliation: Research and Development Center, PETROBRAS, Ilha do Fundão, Rio de Janeiro, Brazil.
authors.forename: Aline Machado
authors.initials: AM
authors.lastname: de Castro
authors.forename: Kelly Cristina Nascimento Rodrigues
authors.initials: KC
authors.lastname: Pedro
authors.forename: Juliana Cunha
authors.initials: JC
authors.lastname: da Cruz
authors.forename: Marcela Costa
authors.initials: MC
authors.lastname: Ferreira
authors.forename: Selma Gomes Ferreira
authors.initials: SG
authors.lastname: Leite
authors.forename: Nei
authors.initials: N
authors.lastname: Pereira
mesh_terms: D044602:Cellulases / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D004795:Enzyme Stability; D005285:Fermentation; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D007220:Industrial Waste / Q000032:analysis; D031786:Saccharum / Q000382:microbiology*; D014242:Trichoderma / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D007220:Industrial Waste; D002482:Cellulose; C027433:bagasse; D044602:Cellulases