Breeding of a low pyruvate-producing sake yeast by isolation of a mutant resistant to ethyl alpha-transcyanocinnamate, an inhibitor of mitochondrial pyruvate transport.
Pyruvate is the key substance controlling the formation of diacetyl, acetaldehyde, and acetate during alcoholic fermentation. Here we report the breeding of a low pyruvate-producing sake yeast by isolation of a mutant resistant to ethyl alpha-transcyanocinnamate, an inhibitor of mitochondrial pyruvate transport. Mitochondrial function was involved in resistance to this substance and in the production of pyruvate by the mutants.
Horie
Kenta
K
Faculty of Agriculture, Saga University, Japan.
Oba
Takahiro
T
Motomura
Saori
S
Isogai
Atsuko
A
Yoshimura
Takashi
T
Tsuge
Keisuke
K
Koganemaru
Kazuyoshi
K
Kobayashi
Genta
G
Kitagaki
Hiroshi
H
D000085:Acetates / Q000378:metabolism; D000434:Alcoholic Beverages; D000435:Alcoholic Intoxication / Q000235:genetics / Q000378:metabolism; D057229:Alcoholics; D001692:Biological Transport / Q000235:genetics; D001947:Breeding; D005285:Fermentation / Q000235:genetics; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D015003:Yeasts / Q000235:genetics
D000085:Acetates; D011773:Pyruvates; D019289:Pyruvic Acid