Gene expression profiling of breast cancer cell lines in response to soy isoflavones using a pangenomic microarray approach.
Although the rate of breast cancer differs between women in Asian and Western countries, molecular genetics/genomics basis of this epidemiological observation remains elusive. Moreover, the intake of phytoestrogens is associated with a lower incidence of breast cancer. Genistein and daidzein are the primary soy isoflavones with a chemical structure similar to estrogens. Conceivably, the actions of phytoestrogens on gene expression signatures might mediate their postulated effects on breast cancer pathogenesis. The present study evaluated the transcriptional responsiveness of breast cancer cells to soy phytoestrogens using a whole-genome microarray-based approach. Human breast cancer cell lines and a fibrocystic breast cell line were treated with genistein or daidzein. We identified 278 and 334 differentially expressed genes after genistein or daidzein treatment, respectively, in estrogen-positive (MCF-7) and estrogen-negative (MDA-MB-231, MCF-10a) cells. Hierarchical clustering of this finding revealed a significant modulation, respectively, of 246 or 169 genes after genistein or daidzein exposures. Importantly, the molecular pathways for the differentially expressed genes included those that relate to cell communication, biodegradation of xenobiotics, lipid metabolism, signal transduction, and cell growth/death. These molecular observations collectively contribute to a growing knowledgebase on the putative mechanism(s) of action of phytoestrogens in breast cancer pathogenesis and chemoprevention.
Satih
Samir
S
Centre Jean Perrin, Département d'Oncogénétique, Clermont-Ferrand, France.
Chalabi
Nasséra
N
Rabiau
Nadège
N
Bosviel
Rémy
R
Fontana
Luc
L
Bignon
Yves-Jean
YJ
Bernard-Gallon
Dominique J
DJ
D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D019833:Genistein / Q000494:pharmacology*; D015894:Genome, Human; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D046228:Microarray Analysis / Q000379:methods*; D008969:Molecular Sequence Data; D005810:Multigene Family; D048789:Phytoestrogens / Q000494:pharmacology*
D007529:Isoflavones; D048789:Phytoestrogens; C004742:daidzein; D019833:Genistein
J Natl Cancer Inst. 2006 Sep 20;98(18):1275-84
16985246
Biochim Biophys Acta. 2007 Feb;1769(2):124-30
17321611
Endocr Relat Cancer. 2008 Mar;15(1):161-73
18310284
FEBS Lett. 2005 Mar 14;579(7):1732-40
15757668
J Natl Cancer Inst. 2006 Apr 5;98(7):430-1
16595771
Bioinformatics. 2004 Dec 12;20(18):3705-6
15297296
Oncogene. 2008 Feb 28;27(10):1429-38
17891184
Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21
11309499
Nutr Cancer. 2003;45(2):247-55
12881020
Int J Cancer. 1999 May 31;81(5):682-7
10328216
BMC Cancer. 2008 Feb 04;8:41
18248671
Mol Biol Cell. 2008 Sep;19(9):3745-57
18579687
Bioinformatics. 2003 May 1;19(7):825-33
12724292
Rev Epidemiol Sante Publique. 2003 Feb;51(1 Pt 1):3-30
12684578
Mol Cell Biol. 2000 Mar;20(5):1723-32
10669749
Curr Opin Mol Ther. 2007 Dec;9(6):538-44
18041664
Br J Cancer. 2008 Jan 15;98(1):9-14
18182974
Endocr Relat Cancer. 2006 Dec;13(4):995-1015
17158751
Endocr Relat Cancer. 2006 Mar;13(1):169-80
16601286
J Natl Cancer Inst. 2006 Apr 5;98(7):459-71
16595782
Drug Metab Dispos. 2008 Aug;36(8):1594-605
18480186
Breast Cancer Res Treat. 2001 Nov;70(1):47-54
11767004
Pharmacogenomics. 2006 Jul;7(5):663-72
16886892
J Natl Cancer Inst. 1973 Nov;51(5):1409-16
4357757
J Natl Cancer Inst. 1974 Sep;53(3):661-74
4412247
Drug Metab Dispos. 2007 Oct;35(10):1935-41
17646279
Cancer Res. 1990 Sep 15;50(18):6075-86
1975513
Ann Med. 2005;37(6):423-38
16203615
Neoplasia. 2007 Feb;9(2):147-58
17356711
Genome Biol. 2003;4(10):R70
14519205
J Pharmacol Exp Ther. 1999 Jun;289(3):1611-9
10336559
Prostaglandins Other Lipid Mediat. 2007 Nov;84(3-4):116-27
17991614
Thorax. 2005 Jul;60(7):576-81
15994266
Med Hypotheses. 2006;66(6):1093-114
16513288
Endocrinology. 1998 Oct;139(10):4252-63
9751507
Steroids. 2004 Dec;69(13-14):795-801
15582534
Mol Carcinog. 2006 May;45(5):320-32
16526022
Mol Pharmacol. 2001 Sep;60(3):595-602
11502892
Adv Exp Med Biol. 2008;617:625-32
18497090