title: Enhanced microparticles in ventricular assist device patients predict platelet, leukocyte and endothelial cell activation.
abstract: Platelet dysfunction followed by thromboembolic as well as bleeding complications are severe events in patients with ventricular assist devices (VADs). Microparticles (MP) are cell vesicles with inflammatory and coagulatory potency that are released by cells during activation and can be used as specific cell activation markers. The aim of the study was to establish MP as surrogate markers for platelet, leukocyte and endothelial activation in VAD patients predicting platelet activation and vascular inflammation. Therefore, MP from platelet, leukocyte and endothelial origin were measured in 12 patients with VAD and compared to controls. VAD patients had significantly increased levels of platelet microparticles (PMP) (VAD 1705+/-1100 cpm vs. co: 527+/-238 cpm, P=0.002), leukocyte microparticles (LMP) (VAD 43+/-16 cpm vs. co: 18+/-8 cpm, P<0.001) and endothelial microparticles (EMP) (VAD 135+/-66 cpm vs. co: 46+/-23 cpm, P<0.001) indicating enhanced vascular inflammation and pro-coagulation. Furthermore, LMP correlated well to EMP suggesting that LMP induce endothelial cell dysfunction. In conclusion, VAD patients suffer from enhanced platelet, leukocyte and endothelial activation that can be easily quantified by measuring PMP, LMP and EMP and that may help to estimate the thrombotic risk and contribute to avoid ischemic but also bleeding complications.
authors.affiliation: Department of Cardiology and Angiology, University-Hospital Freiburg, Hugstetterstr. 55, 79106 Freiburg, Germany. philipp.diehl@uniklinik-freiburg.de
authors.forename: Philipp
authors.initials: P
authors.lastname: Diehl
authors.forename: Miriam
authors.initials: M
authors.lastname: Aleker
authors.forename: Thomas
authors.initials: T
authors.lastname: Helbing
authors.forename: Verena
authors.initials: V
authors.lastname: Sossong
authors.forename: Friedhelm
authors.initials: F
authors.lastname: Beyersdorf
authors.forename: Manfred
authors.initials: M
authors.lastname: Olschewski
authors.forename: Christoph
authors.initials: C
authors.lastname: Bode
authors.forename: Martin
authors.initials: M
authors.lastname: Moser
mesh_terms: D000328:Adult; D000368:Aged; D001777:Blood Coagulation; D001792:Blood Platelets / Q000473:pathology*; D016022:Case-Control Studies; D055252:Cell-Derived Microparticles / Q000473:pathology*; D042783:Endothelial Cells / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D005858:Germany; D006353:Heart-Assist Devices / Q000009:adverse effects*; D006470:Hemorrhage / Q000097:blood / Q000209:etiology* / Q000473:pathology; D006801:Humans; D007962:Leukocytes / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation; D018570:Risk Assessment; D012307:Risk Factors; D013927:Thrombosis / Q000097:blood / Q000209:etiology* / Q000473:pathology