Male-specific (Z)-9-tricosene stimulates female mating behaviour in the spider Pholcus beijingensis.
Chemical signals play an important role in spider sexual communication, yet the chemistry of spider sex pheromones remains poorly understood. Chemical identification of male-produced pheromone-mediating sexual behaviour in spiders has also, to our knowledge, not been reported before. This study aimed to examine whether chemically mediated strategies are used by males of the spider Pholcus beijingensis for increasing the probability of copulation. Based on data from gas chromatography-mass spectrometry analysis, electroantennography assay and a series of behavioural tests, we verified that (Z)-9-tricosene is a male-specific compound in the spider P. beijingensis. This compound acts as an aphrodisiac: it increases the likelihood that a female will mate. Mate-searching males release (Z)-9-tricosene to stimulate sexual behaviour of conspecific females. In the two-choice assay, however, sexually receptive females show no preference to the chambers containing (Z)-9-tricosene. This indicates that the male pheromone of P. beijingensis is not an attractant per se to the conspecific females. This is, to our knowledge, the first identification of a male-produced aphrodisiac pheromone in spiders.
Xiao
Yong-Hong
YH
Chinese Academy of Sciences, , Beijing, People's Republic of China.
Zhang
Jian-Xu
JX
Li
Shu-Qiang
SQ
D000818:Animals; D002755:Choice Behavior / Q000187:drug effects; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D012724:Sex Attractants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D012726:Sexual Behavior, Animal / Q000187:drug effects*; D013112:Spiders / Q000502:physiology*
D012724:Sex Attractants
J Comp Physiol A. 2001 Feb;187(1):75-8
11318380
Annu Rev Entomol. 2021 Jan 7;66:45-60
33417824
Naturwissenschaften. 2004 Mar;91(3):143-7
15034666
J Chem Ecol. 2009 Jul;35(7):769-78
19579045
Science. 1971 Oct 1;174(4004):76-8
5120874
Angew Chem Int Ed Engl. 2010 Mar 8;49(11):2037-40
20146290
Oecologia. 1983 Jan;56(1):132-139
28310781
Biol Rev Camb Philos Soc. 2007 Feb;82(1):27-48
17313523
J Chem Ecol. 2002 Apr;28(4):835-48
12035930
Angew Chem Int Ed Engl. 2010 Mar 8;49(11):2033-6
20155770
J Chem Ecol. 1996 Jan;22(1):103-21
24226986
Science. 1993 Jun 11;260(5114):1635-7
17810206
J Chem Ecol. 1987 Feb;13(2):237-44
24301803
Gen Comp Endocrinol. 2005 Oct;144(1):60-6
15955534
Science. 1987 May 22;236(4804):964-7
17812752
Naturwissenschaften. 2003 Sep;90(9):410-3
14504784
Angew Chem Int Ed Engl. 2000 Dec 1;39(23):4339-4341
29711932
J Chem Ecol. 1994 May;20(5):1209-16
24242316
Science. 1977 Dec 16;198(4322):1173-5
17818935
PLoS Biol. 2007 Sep;5(9):e228
17713987
J Chem Ecol. 1993 Sep;19(9):1905-16
24249367
Anim Behav. 1998 Nov;56(5):1155-1159
9819331
Naturwissenschaften. 2002 Mar;89(3):111-4
12046629