Protection of erythrocyte membrane against oxidative damage by geriforte in healthy human subjects.
The influence of Geriforte, an Ayurvedic natural supplement, on the antioxidant defense systems in human erythrocytes and plasma was investigated in an open human clinical study. The ability of Geriforte to inhibit the azo-bis 2-amidinopropane dihydrochloride (AAPH) dependent lysis of erythrocytes was also evaluated. Geriforte supplementation increased the activity of erythrocyte catalase (265.745 +/- 15.768 vs. 352,329 +/- 18.480 K/g Hb/mL, p < 0.01) and reduced the free radical mediated cytotoxicity induced by azo-bis 2-amidinopropane dihydrochloride (AAPH), which was measured by erythrocyte membrane stability assay (0.0439 +/- 0.0069 vs. 0.1291 +/- 0.0396 C50- AAPH (mM), p < 0.05). The intervention did not change significantly the activity of erythrocyte superoxide dismutase and the plasma levels of antioxidant agents. The results indicate that Geriforte possesses cytoprotective properties on erythrocytes against oxidative challenge and specific antioxidant activity, which involves mainly the intracellular protective systems and the membranes.
Gîlcă
Marilena
M
Biochemistry Department, Faculty of Medicine, Carol Davila University, Bucharest, Romania. marilenagilca@gmail.com
Stoian
Irina
I
Lixandru
Daniela
D
Găman
Laura
L
Vîrgolici
Bogdana
B
Atanasiu
V
V
D000578:Amidines / Q000494:pharmacology; D019610:Cytoprotection; D019587:Dietary Supplements; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology*; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000097:blood
D000578:Amidines; D016877:Oxidants; D010936:Plant Extracts; C046728:2,2'-azobis(2-amidinopropane); C047351:geriforte; D013482:Superoxide Dismutase