Dynamic flexibility of DNA repair pathways in growth arrested Escherichia coli.
The DNA of all organisms is constantly damaged by exogenous and endogenous agents. Base excision repair (BER) is important for the removal of several non-bulky lesions from the DNA, however not much is known about the contributions of other DNA repair pathways to the processing of non-bulky lesions. Here we utilized a luciferase reporter system to assess the contributions of transcription-coupled repair (TCR), BER and nucleotide excision repair (NER) to the repair of two non-bulky lesions, 8-oxoguanine (8OG) and uracil (U), in vivo under non-growth conditions. We demonstrate that both TCR and NER are utilized by Escherichia coli to repair 8OG and U. Additionally, the relative level of recognition of these lesions by BER and NER suggests that TCR can utilize components of either pathway for lesion removal, depending upon their availability. These findings indicate a dynamic flexibility of DNA repair pathways in the removal of non-bulky DNA lesions in prokaryotes, and reveal their respective contributions to the repair of 8OG and U in vivo.
Clauson
Cheryl L
CL
Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.
Saxowsky
Tina T
TT
Doetsch
Paul W
PW
D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development*; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D008957:Models, Genetic; D014158:Transcription, Genetic; D014498:Uracil / Q000378:metabolism
C024829:8-hydroxyguanine; D014498:Uracil; D006147:Guanine
DNA Repair (Amst). 2002 Jan 22;1(1):59-75
12509297
DNA Repair (Amst). 2008 Oct 1;7(10):1670-9
18707026
Nucleic Acids Res. 2002 Feb 1;30(3):782-93
11809892
Mol Cell Biol. 1999 Apr;19(4):2929-35
10082560
J Biol Chem. 1993 Mar 15;268(8):5849-55
8449951
Mol Syst Biol. 2006;2:2006.0008
16738554
Genetics. 1962 May;47(5):577-85
17248104
Genetics. 2003 Jun;164(2):443-55
12807766
EMBO J. 2006 Nov 29;25(23):5481-91
17110932
DNA Repair (Amst). 2006 Mar 7;5(3):324-35
16337438
Nat Rev Mol Cell Biol. 2008 Dec;9(12):958-70
19023283
Chem Rev. 2006 Feb;106(2):474-88
16464015
Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3657-62
20142484
J Bacteriol. 1985 Nov;164(2):689-95
2997126
J Bacteriol. 1981 Jan;145(1):306-20
6450747
Methods Enzymol. 1991;204:18-43
1943777
J Biol Chem. 1979 Aug 25;254(16):7534-9
381286
J Bacteriol. 1982 Aug;151(2):750-5
7047496
Nature. 2007 Jun 21;447(7147):941-50
17581577
Mutagenesis. 2007 Sep;22(5):343-51
17630408
Curr Protoc Mol Biol. 2001 May;Chapter 1:Unit1.8
18265047
Genome Biol. 2006;7(4):R32
16626502
Mol Cell. 2003 Oct;12(4):959-70
14580346
Biotechniques. 2004 Nov;37(5):760-2, 764, 766
15560132
Methods Enzymol. 2006;409:345-57
16793411
Nucleic Acids Res. 1999 Mar 1;27(5):1365-8
9973627
DNA Repair (Amst). 2004 Nov 2;3(11):1457-68
15380101
J Biol Chem. 1998 Aug 14;273(33):21276-81
9694887
J Biol Chem. 2001 Nov 30;276(48):45367-71
11571287