Erratum to: rhesus macaque MHC class I molecules show differential subcellular localizations.
The MHC class I gene family of rhesus macaques is characterised by considerable gene duplications. While a HLA-C-orthologous gene is absent, the Mamu-A and in particular the Mamu-B genes have expanded, giving rise to plastic haplotypes with differential gene content. Although some of the rhesus macaque MHC class I genes are known to be associated with susceptibility/resistance to infectious diseases, the functional significance of duplicated Mamu-A and Mamu-B genes and the expression pattern of their encoded proteins are largely unknown. Here, we present data of the subcellular localization of AcGFP-tagged Mamu-A and Mamu-B molecules. We found strong cell surface and low intracellular expression for Mamu-A1, Mamu-A2 and Mamu-A3-encoded molecules as well as for Mamu-B*01704, Mamu-B*02101, Mamu-B*04801, Mamu-B*06002 and Mamu-B*13401. In contrast, weak cell surface and strong intracellular expression was seen for Mamu-A4*1403, Mamu-B*01202, Mamu-B*02804, Mamu-B*03002, Mamu-B*05704, Mamu-I*010201 and Mamu-I*0121. The different expression patterns were assigned to the antigen-binding alpha1 and alpha2 domains, suggesting failure of peptide binding is responsible for retaining 'intracellular' Mamu class I molecules in the endoplasmic reticulum. These findings indicate a diverse functional role of the duplicated rhesus macaque MHC class I genes.
Rosner
Cornelia
C
Abteilung Primatengenetik, Deutsches Primatenzentrum-Leibniz, Institut für Primatenforschung, Kellnerweg 4, 37077, Göttingen, Germany.
Kruse
Philip H
PH
Lübke
Torben
T
Walter
Lutz
L
D000818:Animals; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D020014:K562 Cells; D008253:Macaca mulatta / Q000235:genetics / Q000276:immunology*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000032:analysis
D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger
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