[Effect of xuefu zhuyu decoction in inducing angiogenesis gene regulation of endothelial cell line ECV304].
OBJECTIVE
To study the acting mechanism of endothelial cell line ECV304 in regulating angiogenesis induced by Xuefu Zhuyu Decoction (XFZY).


METHODS
The angiogenesis effect of XFZY-contained serum (XFZY-CS) was confirmed by observing its impact on proliferation, cell cycle, migration of ECV304 and on vascular neogenesis in vitro. Then the effect of XFZY on various angiogenesis controlling factors was analyzed with gene chip microarray technique.


RESULTS
Treatment of XFZY-CS in 2.5% concentration for 48 h showed evident actions of enhancing ECV304 activity, increasing cell numbers of S phase, inducing cell migration and promoting the in vitro angiogenesis. Meanwhile, expressions of four angiogenesis controlling genes were up-regulated and 10 were down-regulated.


CONCLUSIONS
The angiogenesis mechanism of ECV304 induced by XFZY is complex, it shows a multi-pathway and multi-target feature.
Gao
Dong
D
Fujian College of Traditional Chinese Medicine, Fuzhou.
Chen
Wen-yuan
WY
Wu
Li-ya
LY
D043925:Angiogenesis Inducing Agents; D000818:Animals; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D043925:Angiogenesis Inducing Agents; D004365:Drugs, Chinese Herbal; C513826:Xue-Fu-Zhu-Yu decoction