Ulvan, a sulfated polysaccharide from green algae, activates plant immunity through the jasmonic acid signaling pathway.
The industrial use of elicitors as alternative tools for disease control needs the identification of abundant sources of them. We report on an elicitor obtained from the green algae Ulva spp. A fraction containing most exclusively the sulfated polysaccharide known as ulvan-induced expression of a GUS gene placed under the control of a lipoxygenase gene promoter. Gene expression profiling was performed upon ulvan treatments on Medicago truncatula and compared to phytohormone effects. Ulvan induced a gene expression signature similar to that observed upon methyl jasmonate treatment (MeJA). Involvement of jasmonic acid (JA) in ulvan response was confirmed by detecting induction of protease inhibitory activity and by hormonal profiling of JA, salicylic acid (SA) and abscisic acid (ABA). Ulvan activity on the hormonal pathway was further consolidated by using Arabidopsis hormonal mutants. Altogether, our results demonstrate that green algae are a potential reservoir of ulvan elicitor which acts through the JA pathway.
Jaulneau
Valérie
V
Laboratoire des Surfaces Cellulaires et Signalisation chez les Végétaux, Université de Toulouse, UPS, 24 Chemin de Borde Rouge, BP42617, Auzeville 31326 Castanet-Tolosan, France.
Lafitte
Claude
C
Jacquet
Christophe
C
Fournier
Sylvie
S
Salamagne
Sylvie
S
Briand
Xavier
X
Esquerré-Tugayé
Marie-Thérèse
MT
Dumas
Bernard
B
D000085:Acetates / Q000276:immunology / Q000378:metabolism*; D000704:Analysis of Variance; D017360:Arabidopsis / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism; D002850:Chromatography, Gel; D003517:Cyclopentanes / Q000276:immunology / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D005966:Glucuronidase / Q000378:metabolism; D046913:Medicago truncatula / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins / Q000276:immunology / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D044445:Ulva / Q000737:chemistry*; D010453:Peptide Synthases
D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005966:Glucuronidase; D009713:Nucleotidyltransferases; D054883:Oxylipins; D010937:Plant Growth Regulators; D011134:Polysaccharides; C072239:methyl jasmonate; C412831:AT2G46370 protein, Arabidopsis; D010453:Peptide Synthases
Anal Biochem. 1973 Aug;54(2):484-9
4269305
Planta. 2004 Mar;218(5):683-92
14760535
Ann Bot. 2003 Sep;92(3):329-37
12871847
Plant Physiol. 2001 Nov;127(3):1089-101
11706189
Plant Cell Physiol. 2005 Dec;46(12):1964-72
16215271
Blood. 1998 Jul 1;92(1):223-9
9639520
Curr Biol. 2008 May 6;18(9):650-5
18450451
Plant Cell. 2004 Nov;16(11):3020-32
15494557
Planta. 2008 Feb;227(3):539-58
17929052
Plant Cell. 2002 Oct;14(10):2627-41
12368509
New Phytol. 2001 Jan;149(1):43-51
33853239
Cell Calcium. 2005 Dec;38(6):527-38
16198416
Biopolymers. 2009 Aug;91(8):652-64
19353644
Mol Microbiol. 2006 Sep;61(6):1365-71
16899081
Plant Physiol. 1980 Dec;66(6):1128-34
16661590
Plant Cell. 1996 Dec;8(12):2309-23
8989885
Biochim Biophys Acta. 2000 Mar 7;1477(1-2):112-21
10708853
Plant Physiol. 1996 Nov;112(3):997-1004
8938408
Plant Physiol. 2000 Nov;124(3):1027-38
11080280
Mol Plant Microbe Interact. 2008 Feb;21(2):232-43
18184067
Plant Physiol. 2009 Aug;150(4):1750-61
19571312
Plant Physiol. 2007 Jan;143(1):378-88
17085514
J Exp Bot. 2009;60(2):377-90
19073963
Planta. 2008 Jun;228(1):61-78
18327607
Plant Cell. 2004 Dec;16(12):3460-79
15548743
EMBO J. 1987 Dec 20;6(13):3901-7
3327686
Plant Cell. 2007 May;19(5):1665-81
17513501
Carbohydr Res. 1996 Mar 22;283:161-73
8901268
Int Immunopharmacol. 2007 Jul;7(7):879-88
17499190
Plant Physiol Biochem. 2010 Apr;48(4):225-31
20137961
Plant Methods. 2008 Jun 30;4:16
18590529
Science. 2009 May 8;324(5928):750-2
19423816
Mol Plant Microbe Interact. 2003 Feb;16(2):115-22
12575745
Curr Opin Immunol. 2008 Feb;20(1):10-6
18206360
Biomacromolecules. 2007 Jun;8(6):1765-74
17458931
Plant Mol Biol. 2004 Jan;54(2):261-72
15159627
Plant Biol (Stuttg). 2006 Jan;8(1):1-10
16435264
Plant Cell Environ. 2009 Feb;32(2):178-193
19021887
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Nature. 2002 Feb 28;415(6875):977-83
11875555
J Gen Physiol. 1948 Nov;32(2):241-63
18891149