[Gene characteristic of VP1 region of poliovirus isolates from acute flaccid paralysis in Hebei Province].
OBJECTIVE
In order to provide the evidence for polio free status, the VP1 gene characteristic analysis of poliovirus isolated from acute flaccid paralysis (AFP) cases and close contacts were conducted in Hebei Province during 2007-2008.


METHODS
Virus isolation and identification were conducted according to the 4th edition of WHO polio laboratory manual. The sequence of VP1 region were amplified and sequenced. The phylogenetic trees based on VP1 region were constructed.


RESULTS
The total number of poliovirus isolates were 42. Most isolates were type II and III. No wild poliovirus and vaccine derived poliovirus (VDPVs) was detected.


CONCLUSIONS
Gene characteristic of VP1 region of poliovirus can be used to identify the polio virus transmission and guide the work of immunization program.
Chen
Mei
M
Hebei Provincial Center for Disease Control and Prevention, Shijiazhuang 050021, Hebei, China.
Yan
Dong-Mei
DM
Zhu
Hui
H
D000818:Animals; D036022:Capsid Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002681:China / Q000453:epidemiology; D006801:Humans; D051379:Mice; D010264:Paraplegia / Q000453:epidemiology / Q000821:virology*; D010802:Phylogeny; D017955:Poliovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
D036022:Capsid Proteins; C442540:VP1 protein, Poliovirus