title: Effect of gallium-arsenide laser, gallium-aluminum-arsenide laser and healing ointment on cutaneous wound healing in Wistar rats.
abstract: This study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals' backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm(2)); group 2 (L30): GaAlAs laser (30 J/cm(2)); group 3 (L60): GaAlAs laser (60 J/cm(2)); group 4 (D): Dersani ointment; group 5 (control): 0.9% saline. The applications were made daily over a period of 20 days. Tissue fragments were stained with picrosirius to distinguish type I collagen from type III collagen. The collagen fibers were photo-documented and analyzed using the Quantum software based on the primary color spectrum (red, yellow and blue). Significant results for wound closing rate were obtained for group 1 (L4), 7.37 mm/day. The highest concentration of type III collagen fibers was observed in group 2 (L30; 37.80 + or - 7.10%), which differed from control (29.86 + or - 5.15%) on the 20th day of treatment. The type I collagen fibers of group 1 (L4; 2.67 + or - 2.23%) and group 2 (L30; 2.87 + or - 2.40%) differed significantly from control (1.77 + or - 2.97%) on the 20th day of the experiment.
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Viçosa, Edifício Shotaro Shymoia, Avenida PH Rolfs, s/n, 35570-000 Viçosa, MG, Brasil. reggysvilela@yahoo.com.br
authors.forename: R V
authors.initials: RV
authors.lastname: Gonçalves
authors.forename: J M S
authors.initials: JM
authors.lastname: Mezêncio
authors.forename: G P
authors.initials: GP
authors.lastname: Benevides
authors.forename: S L P
authors.initials: SL
authors.lastname: Matta
authors.forename: C A
authors.initials: CA
authors.lastname: Neves
authors.forename: M M
authors.initials: MM
authors.lastname: Sarandy
authors.forename: E F
authors.initials: EF
authors.lastname: Vilela
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000528:radiation effects*; D024061:Collagen Type III / Q000528:radiation effects*; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy / Q000379:methods; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000528:radiation effects*; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III