title: Simultaneous microemulsion liquid chromatographic analysis of fat-soluble vitamins in pharmaceutical formulations: optimization using genetic algorithm.
abstract: An environmentally benign and simple method has been proposed for separation and determination of fat-soluble vitamins using isocratic microemulsion liquid chromatography. Optimization of parameters affecting the separation selectivity and efficiency including surfactant concentration, percent of cosurfactant (1-butanol), and percent of organic oily solvent (diethyl ether), temperature and pH were performed simultaneously using genetic algorithm method. A new software package, MLR-GA, was developed for this purpose. The results indicated that 73.6mM sodium dodecyl sulfate, 13.64% (v/v) 1-butanol, 0.48% (v/v) diethyl ether, column temperature of 32.5 degrees C and 0.02M phosphate buffer of pH 6.99 are the best conditions for separation of fat-soluble vitamins. At the optimized conditions, the calibration plots for the vitamins were obtained and detection limits (1.06-3.69microgmL(-1)), accuracy (recoveries>94.3), precision (RSD<3.96) and linearity (0.01-10mgmL(-1)) were estimated. Finally, the amount of vitamins in multivitamin syrup and a sample of fish oil capsule were determined. The results showed a good agreement with those reported by manufactures.
authors.affiliation: Department of Chemistry, University of Isfahan, Isfahan 81746-73441, Iran; Chemistry Department, Faculty of Science, Yasouj University, Yasouj 75914-353, Iran. f.momen@chem.ui.ac.ir
authors.forename: Fariborz
authors.initials: F
authors.lastname: Momenbeik
authors.forename: Mostafa
authors.initials: M
authors.lastname: Roosta
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Nikoukar
mesh_terms: D000465:Algorithms; D002214:Capsules / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D004655:Emulsions; D005395:Fish Oils; D006838:Hydrocarbons / Q000737:chemistry; D016014:Linear Models; D008957:Models, Genetic; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D014815:Vitamins / Q000032:analysis* / Q000737:chemistry
chemical_list: D002214:Capsules; D004655:Emulsions; D005395:Fish Oils; D006838:Hydrocarbons; D012997:Solvents; D014815:Vitamins