title: Evaluation of rapid antigen detection kits for diagnosis of equine rotavirus infection.
abstract: We evaluated antigen detection kits for human rotavirus with regard to their usefulness for diagnosing equine rotavirus infection. Limiting dilution analyses showed that of the seven kits investigated the Dipstick `Eiken' Rota (Dipstick) had the highest sensitivity to two serotypes of equine rotavirus. The Dipstick did not cross-react with several equine intestinal pathogens. An investigation using 249 fecal samples indicated that the sensitivity of the Dipstick was 81.9% and 47.3%, and its specificity was 98.2% and 99.0%, and its concordance rate was 92.8% and 68.3%, compared with values obtained using reverse transcription polymerase chain reaction and reverse transcription loop-mediated isothermal amplification, respectively. Although a negative result does not preclude the possibility of equine rotavirus infection, the Dipstick would be useful as routine test for diagnosing equine rotavirus infection in daily clinical practice because of its ease of handling.
authors.affiliation: Epizootic Research Center, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan. nemoto_manabu@epizoo.equinst.go.jp
authors.forename: Manabu
authors.initials: M
authors.lastname: Nemoto
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hata
authors.forename: Tohru
authors.initials: T
authors.lastname: Higuchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Imagawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Niwa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Bannai
authors.forename: Koji
authors.initials: K
authors.lastname: Tsujimura
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Tomio
authors.initials: T
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D005784:Gene Amplification; D005814:Genes, Viral / Q000235:genetics; D006734:Horse Diseases / Q000175:diagnosis / Q000821:virology*; D006736:Horses; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D012401:Rotavirus / Q000235:genetics / Q000276:immunology*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic