title: Efficacy of a new inactivated Chlamydophila felis vaccine in experimentally-infected cats.
abstract: A new inactivated and adjuvanted Chlamydophila felis vaccine was developed and its efficacy in cats was compared with that of commercially available inactivated and live vaccines. Two commercial vaccines conferred insufficient immunity on inoculated cats, as evaluated by antibody production and a challenge experiment, whereas cats administered the newly generated vaccine produced high-titre antibodies and acquired sufficient immunity. The cats immunised with the new vaccine revealed no or only mild clinical signs, and no chlamydiae were recovered from their tissue samples after exposure to a virulent C felis. However, they shed chlamydiae in their nasal and conjunctival secretions after challenge, as did those immunised with the commercial vaccines and the non-vaccinated controls. The newly developed vaccine caused no adverse reaction in the inoculated cats. These findings suggest that the new vaccine prepared here may be promising for practical use in controlling C felis infection in cats.
authors.affiliation: Kyoto Biken Laboratories, Inc., 24-16 Makishima-cho, Uji-shi, Kyoto 611-0041, Japan. caninefeline@kyotobiken.co.jp
authors.forename: Katsuo
authors.initials: K
authors.lastname: Masubuchi
authors.forename: Akira
authors.initials: A
authors.lastname: Wakatsuki
authors.forename: Kayo
authors.initials: K
authors.lastname: Iwamoto
authors.forename: Takuo
authors.initials: T
authors.lastname: Takahashi
authors.forename: Teruaki
authors.initials: T
authors.lastname: Kokubu
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Shimizu
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001428:Bacterial Vaccines / Q000009:adverse effects / Q000276:immunology*; D002371:Cat Diseases / Q000517:prevention & control*; D002415:Cats; D002689:Chlamydia / Q000302:isolation & purification; D023501:Chlamydophila / Q000276:immunology*; D023521:Chlamydophila Infections / Q000517:prevention & control / Q000662:veterinary*; D005260:Female; D008297:Male; D014613:Vaccines, Attenuated / Q000009:adverse effects / Q000276:immunology; D015164:Vaccines, Inactivated / Q000009:adverse effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated; D015164:Vaccines, Inactivated
references.citation: Can J Comp Med. 1980 Jul;44(3):244-51
references.pmid: 6448682
references.citation: J Vet Intern Med. 2003 Nov-Dec;17(6):799-807
references.pmid: 14658715
references.citation: J Am Vet Med Assoc. 1993 Aug 1;203(3):396-405
references.pmid: 8257557
references.citation: Vet Rec. 1983 Dec 3;113(23):535-6
references.pmid: 6364542
references.citation: J Vet Med Sci. 2000 Jul;62(7):801-3
references.pmid: 10945306
references.citation: J Am Vet Med Assoc. 1997 Jan 15;210(2):249-51
references.pmid: 9018362
references.citation: J Vet Med Sci. 2001 Aug;63(8):937-8
references.pmid: 11558555
references.citation: Vet Parasitol. 2005 Sep 5;132(1-2):75-80
references.pmid: 15990233
references.citation: J Vet Med Sci. 2002 Dec;64(12):1165-8
references.pmid: 12520115
references.citation: Can Vet J. 1992 Feb;33(2):134
references.pmid: 17423950
references.citation: Am J Vet Res. 1977 Sep;38(9):1361-3
references.pmid: 921030
references.citation: Avian Dis. 1993 Apr-Jun;37(2):459-66
references.pmid: 8363510
references.citation: Science. 1942 Nov 20;96(2499):475-6
references.pmid: 17743467
references.citation: J Feline Med Surg. 2006 Jun;8(3):207-11
references.pmid: 16476560
references.citation: Zentralbl Veterinarmed B. 1982 Dec;29(10):769-75
references.pmid: 7164671
references.citation: Infect Immun. 1987 Nov;55(11):2653-7
references.pmid: 3666957
references.citation: Microbiol Immunol. 1996;40(10):755-9
references.pmid: 8981349
references.citation: Vet Rec. 1984 Apr 7;114(14):344-6
references.pmid: 6719790
references.citation: J Am Vet Med Assoc. 1994 Nov 15;205(10):1425-9
references.pmid: 7698923
references.citation: Clin Tech Small Anim Pract. 2005 May;20(2):129-34
references.pmid: 15948428
references.citation: Aust Vet J. 1981 Nov;57(11):515-7
references.pmid: 7342937
references.citation: J Am Vet Med Assoc. 1998 Nov 15;213(10):1422-3
references.pmid: 9828933
references.citation: Microbiol Immunol. 2000;44(3):155-60
references.pmid: 10789502
references.citation: J Vet Med Sci. 1992 Aug;54(4):685-92
references.pmid: 1391179
references.citation: N Z Vet J. 1995 Oct;43(5):201-3
references.pmid: 16031850