title: [Molecular genetic analysis of Pseudomonas aeruginosa strains isolated from environment and patients in health care facilities].
abstract: OBJECTIVE
  Analysis of genetic heterogeneity of Pseudomonas aeruginosa strains isolated in and out of hospitals.
  
  
  METHODS
  To study the genetic diversity of 36 strains of P. aeruginosa plasmid analysis, random amplified polymorphic DNA (RAPD) technique as well as polymerase chain reaction for detection of virulence genes algD, lasB, toxA, plcH, plcN, exoS, nan1, and nan2.
  
  
  RESULTS
  Epidemically important strains were found in different ecological niches. It was shown that these virulence factors could play important roles in pathogenesis of infection.
  
  
  CONCLUSIONS
  RAPD technique was effective for analysis of P. aeruginosa isolates. Number of studied typing bands differed between related isolates for each random primer.
authors.forename: V A
authors.initials: VA
authors.lastname: Antonov
authors.forename: V V
authors.initials: VV
authors.lastname: Altukhova
authors.forename: S S
authors.initials: SS
authors.lastname: Savchenko
authors.forename: G A
authors.initials: GA
authors.lastname: Tkachenko
authors.forename: V S
authors.initials: VS
authors.lastname: Zamaraev
authors.forename: S I
authors.initials: SI
authors.lastname: Zhukova
authors.forename: O G
authors.initials: OG
authors.lastname: Kramar'
authors.forename: N L
authors.initials: NL
authors.lastname: Matveeva
authors.forename: O V
authors.initials: OV
authors.lastname: Ostrovskiĭ
authors.forename: G P
authors.initials: GP
authors.lastname: Dudchenko
mesh_terms: D017931:DNA Primers; D006801:Humans; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique; D012426:Russia / Q000453:epidemiology; D012680:Sensitivity and Specificity; D014505:Urban Population; D037521:Virulence Factors / Q000235:genetics
chemical_list: D017931:DNA Primers; D037521:Virulence Factors