title: Cytogenetic map of common bean (Phaseolus vulgaris L.).
abstract: A cytogenetic map of common bean was built by in situ hybridization of 35 bacterial artificial chromosomes (BACs) selected with markers mapping to eight linkage groups, plus two plasmids for 5S and 45S ribosomal DNA and one bacteriophage. Together with three previously mapped chromosomes (chromosomes 3, 4, and 7), 43 anchoring points between the genetic map and the cytogenetic map of the species are now available. Furthermore, a subset of four BAC clones was proposed to identify the 11 chromosome pairs of the standard cultivar BAT93. Three of these BACs labelled more than a single chromosome pair, indicating the presence of repetitive DNA in their inserts. A repetitive distribution pattern was observed for most of the BACs; for 38% of them, highly repetitive pericentromeric or subtelomeric signals were observed. These distribution patterns corresponded to pericentromeric and subtelomeric heterochromatin blocks observed with other staining methods. Altogether, the results indicate that around half of the common bean genome is heterochromatic and that genes and repetitive sequences are intermingled in the euchromatin and heterochromatin of the species.
authors.affiliation: Laboratory of Plant Cytogenetics, Department of Botany, Federal University of Pernambuco, Recife, PE, 50670-420, Brazil.
authors.forename: Artur
authors.initials: A
authors.lastname: Fonsêca
authors.forename: Joana
authors.initials: J
authors.lastname: Ferreira
authors.forename: Tiago Ribeiro Barros
authors.initials: TR
authors.lastname: dos Santos
authors.forename: Magdalena
authors.initials: M
authors.lastname: Mosiolek
authors.forename: Elisa
authors.initials: E
authors.lastname: Bellucci
authors.forename: James
authors.initials: J
authors.lastname: Kami
authors.forename: Paul
authors.initials: P
authors.lastname: Gepts
authors.forename: Valérie
authors.initials: V
authors.lastname: Geffroy
authors.forename: Dieter
authors.initials: D
authors.lastname: Schweizer
authors.forename: Karla G B
authors.initials: KG
authors.lastname: dos Santos
authors.forename: Andrea
authors.initials: A
authors.lastname: Pedrosa-Harand
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D020732:Cytogenetic Analysis; D022041:Euchromatin / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics*; D006570:Heterochromatin / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D022041:Euchromatin; D006570:Heterochromatin
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