title: Herpes simplex virus type 1 tegument protein VP22 is capable of modulating the transcription of viral TK and gC genes via interaction with viral ICP0.
abstract: VP22, a tegument protein of herpes simplex virus type 1 (HSV-1), is present in many copies in one virion and undergoes different types of post-translational modification. VP22 is believed to have certain functions in viral infection apart from virus assembly. Here we show that VP22 physically interacted with infected cell polypeptide 0 (ICP0) and colocalized in the nucleus, indicating that VP22 could be functionally involved in the modulation of viral transcription through interaction with ICP0. In the HSV-1 infection system and chloramphenicol acetyltransferase (CAT) transcriptional system, VP22-ICP0 interaction was confirmed to play a role in modulating the transcription of some viral genes and could be a factor in viral transcription, which is probably required in the transcriptional control of latent infection.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medicine Science, Peking Union Medical College, Kunming 650118, PR China.
authors.forename: Xian
authors.initials: X
authors.lastname: Yu
authors.forename: Longding
authors.initials: L
authors.lastname: Liu
authors.forename: Lianqiu
authors.initials: L
authors.lastname: Wu
authors.forename: Lichun
authors.initials: L
authors.lastname: Wang
authors.forename: Chenghong
authors.initials: C
authors.lastname: Dong
authors.forename: Weizhong
authors.initials: W
authors.lastname: Li
authors.forename: Qihan
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002522:Chlorocebus aethiops; D017931:DNA Primers; D005814:Genes, Viral; D017874:Immediate-Early Proteins / Q000235:genetics*; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D013937:Thymidine Kinase / Q000235:genetics*; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000502:physiology*
chemical_list: D017931:DNA Primers; D017874:Immediate-Early Proteins; D015678:Viral Structural Proteins; C073413:herpes simplex virus type 1 protein VP22; D015500:Chloramphenicol O-Acetyltransferase; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1; D013937:Thymidine Kinase