title: Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43.
abstract: Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
authors.forename: Hyun Kyoung
authors.initials: HK
authors.lastname: Lee
authors.forename: Byoung Hee
authors.initials: BH
authors.lastname: Lee
authors.forename: Seung Hyeok
authors.initials: SH
authors.lastname: Seok
authors.forename: Min Won
authors.initials: MW
authors.lastname: Baek
authors.forename: Hui Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Dong Jae
authors.initials: DJ
authors.lastname: Kim
authors.forename: Yi Rang
authors.initials: YR
authors.lastname: Na
authors.forename: Kyoung Jin
authors.initials: KJ
authors.lastname: Noh
authors.forename: Sung Hoon
authors.initials: SH
authors.lastname: Park
authors.forename: Dutta Noton
authors.initials: DN
authors.lastname: Kumar
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kariwa
authors.forename: Mina
authors.initials: M
authors.lastname: Nakauchi
authors.forename: Suk Jin
authors.initials: SJ
authors.lastname: Heo
authors.forename: Jae Hak
authors.initials: JH
authors.lastname: Park
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology*; D015153:Blotting, Western; D028941:Coronavirus 229E, Human / Q000276:immunology*; D028962:Coronavirus OC43, Human / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins
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