Generating knockout rats by transposon mutagenesis in spermatogonial stem cells.
Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat.
Izsvák
Zsuzsanna
Z
Max Delbrück Center for Molecular Medicine, Berlin, Germany. zivics@mdc-berlin.de
Fröhlich
Janine
J
Grabundzija
Ivana
I
Shirley
James R
JR
Powell
Heather M
HM
Chapman
Karen M
KM
Ivics
Zoltán
Z
Hamra
F Kent
FK
D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D055786:Gene Knockout Techniques / Q000379:methods*; D008297:Male; D016296:Mutagenesis; D051381:Rats / Q000235:genetics*; D013093:Spermatogonia / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*
D004251:DNA Transposable Elements
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