Microarrays of recombinant Hevea brasiliensis proteins: a novel tool for the component-resolved diagnosis of natural rubber latex allergy.
BACKGROUND
Component-resolved diagnosis using microarray technology has recently been introduced in clinical allergology, but its applicability in patients with natural rubber latex (NRL) allergy has not been investigated.


OBJECTIVE
To evaluate the utility of microarray-based immunoglobulin (Ig) E detection in the diagnostic workup of NRL allergy and to compare this new diagnostic tool with established methods of NRL-specific IgE detection.


METHODS
We investigated 52 adults with immediate-type NRL allergy and 50 control patients. Determination of specific serum IgE against 8 recombinant Hevea brasiliensis allergen components was performed using a customized allergen microarray and a conventional fluorescence enzyme immunoassay (FEIA).


RESULTS
The panel of microarrayed allergen components was shown to represent a comprehensive repertoire of clinically relevant NRL proteins. NRL-specific IgE recognition patterns and sensitization rates determined by microarray analysis were similar to those obtained by conventional FEIA. The diagnostic sensitivity rates of combined single-component data were not significantly different for the respective recombinant test system, whereas the sensitivity level of extract-based FEIA analysis was markedly higher.


CONCLUSIONS
The current study provides evidence that microarrays of recombinant NRL allergen components are a suitable new tool for the diagnosis of NRL-specific sensitization.They show performance characteristics comparable to those of current diagnostic tests and could be indicated in small children in whom only limited blood volumes are obtainable. Further large-scale studies in unselected patient populations and in high-risk groups are warranted before the microarray can be introduced into routine management of patients with NRL allergy.
Ott
H
H
Department of Dermatology and Allergology, University Hospital Aachen, Aachen, Germany. hagen.ott@post.rwth-aachen.de
Schröder
C
C
Raulf-Heimsoth
M
M
Mahler
V
V
Ocklenburg
C
C
Merk
H F
HF
Baron
J M
JM
D000328:Adult; D000368:Aged; D052179:Antigens, Plant / Q000276:immunology; D018450:Disease Progression; D000939:Epitopes / Q000378:metabolism; D005260:Female; D016231:Fluorescence Polarization Immunoassay; D028482:Hevea / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D020315:Latex Hypersensitivity / Q000175:diagnosis* / Q000276:immunology / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D011994:Recombinant Proteins / Q000276:immunology
D052179:Antigens, Plant; D000939:Epitopes; D011994:Recombinant Proteins; D007073:Immunoglobulin E