title: Development of a gene transfer system for the mycelia of Flammulina velutipes Fv-1 strain.
abstract: To develop a gene transformation method for Flammulina velutipes, we constructed a vector with hph gene under control of the trp1 gene promoter. The vector was integrated into protoplast derived from mycelia by the calcium-polyethylene glycol method, as it has not been reported for F. velutipes. Transformation efficiency was much improved when transformation was performed by the restriction enzyme mediated integration method.
authors.affiliation: Food Biotechnology Division, National Food Research Institute, Tsukuba, Ibaraki, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Maehara
authors.forename: Makoto
authors.initials: M
authors.lastname: Yoshida
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Shizuko
authors.initials: S
authors.lastname: Tomita
authors.forename: Koji
authors.initials: K
authors.lastname: Takabatake
authors.forename: Hitomi
authors.initials: H
authors.lastname: Ichinose
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kaneko
mesh_terms: D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D055393:Flammulina / Q000166:cytology / Q000235:genetics*; D018014:Gene Transfer Techniques; D025282:Mycelium / Q000235:genetics*; D011092:Polyethylene Glycols / Q000737:chemistry; D011523:Protoplasts / Q000378:metabolism
chemical_list: D011092:Polyethylene Glycols; D002118:Calcium