title: Inflammatory responses improve with milk ribonuclease-enriched lactoferrin supplementation in postmenopausal women.
abstract: OBJECTIVE
  A 6-month, randomized clinical study was conducted to evaluate the effect of a ribonuclease-enriched lactoferrin (R-ELF) supplement on the circulating cytokine levels and bone health of postmenopausal women.
  
  
  METHODS
  Thirty-eight healthy postmenopausal women, aged 45-60 years, were randomized into placebo and R-ELF groups.
  
  
  METHODS
  The R-ELF group was supplemented with R-ELF (2 × 125 mg/day) and calcium (100% RDA), while the placebo group received only the calcium supplement.
  
  
  METHODS
  Serum levels of receptor activator for NF-κB ligand (RANKL), C-reactive protein (CRP) and various pro- and anti-inflammatory cytokines were determined by ELISA.
  
  
  RESULTS
  Pro-inflammatory cytokines IL-6 and TNF-α decreased significantly (-44 and -10%, respectively) while anti-inflammatory IL-10 increased (140%) due to R-ELF supplementation at the end of study. RANKL and CRP were modestly reduced (-50%) relative to their placebo levels, although RANKL elevated initially.
  
  
  CONCLUSIONS
  R-ELF supplementation showed beneficial effects towards improvement of inflammatory status in postmenopausal women.
authors.affiliation: N-terminus Research Laboratory, Pomona, CA 91768, USA.
authors.forename: Satish
authors.initials: S
authors.lastname: Bharadwaj
authors.forename: Tezus A G
authors.initials: TA
authors.lastname: Naidu
authors.forename: Guru V
authors.initials: GV
authors.lastname: Betageri
authors.forename: Nemani V
authors.initials: NV
authors.lastname: Prasadarao
authors.forename: A Satyanarayan
authors.initials: AS
authors.lastname: Naidu
mesh_terms: D000818:Animals; D002417:Cattle; D016207:Cytokines / Q000097:blood / Q000276:immunology; D019587:Dietary Supplements; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000276:immunology*; D007781:Lactoferrin / Q000008:administration & dosage / Q000737:chemistry; D008875:Middle Aged; D008892:Milk / Q000201:enzymology*; D010919:Placebos; D017698:Postmenopause; D012260:Ribonucleases / Q000008:administration & dosage*
chemical_list: D016207:Cytokines; D010919:Placebos; D012260:Ribonucleases; D007781:Lactoferrin
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