title: Peptide-based fluorescent biosensors.
abstract: The use of fluorescent techniques in biological research is widespread. Many of the techniques rely on the use of fluorescent genetically-encoded tags (namely GFP and its different variants), but small molecules and nanoparticle-based approaches are being increasingly used. Peptides, owing to their modular nature, synthetic accessibility and biomolecular recognition potential, offer unique possibilities for the development of efficient and selective fluorescent sensors. In this tutorial review we present several of the strategies that have been used to develop fluorescent-based peptide sensors and discuss selected applications to biological problems.
authors.affiliation: Departamento de Química Orgánica, Universidade de Santiago de Compostela, Av. de las Ciencias sn, Campus Sur. 15782 Santiago de Compostela, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Pazos
authors.forename: Olalla
authors.initials: O
authors.lastname: Vázquez
authors.forename: José L
authors.initials: JL
authors.lastname: Mascareñas
authors.forename: M Eugenio
authors.initials: ME
authors.lastname: Vázquez
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D010455:Peptides