title: Bacterial communities of two ubiquitous Great Barrier Reef corals reveals both site- and species-specificity of common bacterial associates.
abstract: BACKGROUND
  Coral-associated bacteria are increasingly considered to be important in coral health, and altered bacterial community structures have been linked to both coral disease and bleaching. Despite this, assessments of bacterial communities on corals rarely apply sufficient replication to adequately describe the natural variability. Replicated data such as these are crucial in determining potential roles of bacteria on coral.
  
  
  RESULTS
  Denaturing Gradient Gel Electrophoresis (DGGE) of the V3 region of the 16S ribosomal DNA was used in a highly replicated approach to analyse bacterial communities on both healthy and diseased corals. Although site-specific variations in the bacterial communities of healthy corals were present, host species-specific bacterial associates within a distinct cluster of gamma-proteobacteria could be identified, which are potentially linked to coral health. Corals affected by "White Syndrome" (WS) underwent pronounced changes in their bacterial communities in comparison to healthy colonies. However, the community structure and bacterial ribotypes identified in diseased corals did not support the previously suggested theory of a bacterial pathogen as the causative agent of the syndrome.
  
  
  CONCLUSIONS
  This is the first study to employ large numbers of replicated samples to assess the bacterial communities of healthy and diseased corals, and the first culture-independent assessment of bacterial communities on WS affected Acroporid corals on the GBR. Results indicate that a minimum of 6 replicate samples are required in order to draw inferences on species, spatial or health-related changes in community composition, as a set of clearly distinct bacterial community profiles exist in healthy corals. Coral bacterial communities may be both site and species specific. Furthermore, a cluster of gamma-proteobacterial ribotypes may represent a group of specific common coral and marine invertebrate associates. Finally, the results did not support the contention that a single bacterial pathogen may be the causative agent of WS Acroporids on the GBR.
authors.affiliation: Centre for Marine Studies, The University of Queensland, Brisbane, Queensland, Australia. c.kvennefors@uq.edu.au
authors.forename: E Charlotte E
authors.initials: EC
authors.lastname: Kvennefors
authors.forename: Eugenia
authors.initials: E
authors.lastname: Sampayo
authors.forename: Tyrone
authors.initials: T
authors.lastname: Ridgway
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Barnes
authors.forename: Ove
authors.initials: O
authors.lastname: Hoegh-Guldberg
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D001419:Bacteria / Q000302:isolation & purification*; D008386:Marine Biology; D012336:RNA, Ribosomal, 16S; D021521:Ribotyping; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S
references.citation: Environ Microbiol. 2003 May;5(5):370-82
references.pmid: 12713463
references.citation: Appl Environ Microbiol. 2003 Apr;69(4):2409-13
references.pmid: 12676731
references.citation: Syst Biol. 2001 Aug;50(4):470-8
references.pmid: 12116647
references.citation: Mol Biol Evol. 2005 Mar;22(3):570-81
references.pmid: 15537807
references.citation: Appl Environ Microbiol. 2008 Dec;74(24):7828-31
references.pmid: 18931299
references.citation: Microbiol Mol Biol Rev. 1998 Dec;62(4):1353-70
references.pmid: 9841675
references.citation: Appl Environ Microbiol. 2004 Oct;70(10):5955-62
references.pmid: 15466538
references.citation: Appl Environ Microbiol. 2006 Apr;72(4):3016-20
references.pmid: 16598010
references.citation: ISME J. 2009 May;3(5):512-21
references.pmid: 19129866
references.citation: PLoS Biol. 2004 Apr;2(4):E120
references.pmid: 15094816
references.citation: Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8725-30
references.pmid: 12077296
references.citation: Science. 2002 Jun 21;296(5576):2158-62
references.pmid: 12077394
references.citation: FEMS Microbiol Ecol. 2006 Oct;58(1):99-108
references.pmid: 16958911
references.citation: Microb Ecol. 2007 Nov;54(4):730-9
references.pmid: 17393054
references.citation: Electrophoresis. 1998 Apr;19(4):554-68
references.pmid: 9588802
references.citation: Crit Rev Microbiol. 2000;26(1):37-57
references.pmid: 10782339
references.citation: Appl Environ Microbiol. 2008 Jul;74(13):4231-5
references.pmid: 18456855
references.citation: Appl Environ Microbiol. 1997 Dec;63(12):4645-50
references.pmid: 9406382
references.citation: FEMS Microbiol Ecol. 2009 Mar;67(3):371-80
references.pmid: 19161430
references.citation: ISME J. 2008 Jan;2(1):67-73
references.pmid: 18059488
references.citation: Lett Appl Microbiol. 2002;34(5):359-64
references.pmid: 11967059
references.citation: FEMS Microbiol Lett. 2009 Mar;292(2):210-5
references.pmid: 19191871
references.citation: J Appl Microbiol. 2008 Nov;105(5):1658-71
references.pmid: 18798767
references.citation: Appl Environ Microbiol. 2008 May;74(9):2717-27
references.pmid: 18310425
references.citation: Trends Ecol Evol. 1998 Nov 1;13(11):438-43
references.pmid: 21238385
references.citation: FEMS Microbiol Ecol. 2009 May;68(2):152-63
references.pmid: 19302548
references.citation: Int J Syst Evol Microbiol. 2006 Feb;56(Pt 2):365-368
references.pmid: 16449441
references.citation: Environ Microbiol. 2002 Jul;4(7):401-13
references.pmid: 12123476
references.citation: Appl Environ Microbiol. 2003 Feb;69(2):852-60
references.pmid: 12571004
references.citation: FEMS Microbiol Ecol. 2009 Jan;67(1):103-17
references.pmid: 19120461
references.citation: Environ Microbiol. 2003 Aug;5(8):650-9
references.pmid: 12871232
references.citation: Science. 2004 Aug 13;305(5686):997-1000
references.pmid: 15310901
references.citation: Dis Aquat Organ. 2006 Mar 23;69(1):79-88
references.pmid: 16703769
references.citation: Environ Microbiol. 2007 May;9(5):1291-305
references.pmid: 17472641
references.citation: Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):13146-51
references.pmid: 17664430
references.citation: Environ Microbiol. 2004 Mar;6(3):288-300
references.pmid: 14871212
references.citation: ISME J. 2008 Apr;2(4):350-63
references.pmid: 18059490
references.citation: Microb Ecol. 2009 Jan;57(1):117-28
references.pmid: 18535850
references.citation: Appl Environ Microbiol. 1993 Mar;59(3):695-700
references.pmid: 7683183
references.citation: Syst Appl Microbiol. 2008 Sep;31(4):312-9
references.pmid: 18678453
references.citation: PLoS One. 2008 Jun 18;3(6):e2393
references.pmid: 18560584
references.citation: Int J Syst Evol Microbiol. 2001 Jul;51(Pt 4):1383-1388
references.pmid: 11491336
references.citation: Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):1115-1122
references.pmid: 12892136
references.citation: Appl Environ Microbiol. 2002 Oct;68(10):5142-50
references.pmid: 12324365
references.citation: Appl Environ Microbiol. 1997 Mar;63(3):983-9
references.pmid: 9055415
references.citation: Am Nat. 2003 Oct;162(4 Suppl):S51-62
references.pmid: 14583857
references.citation: Syst Appl Microbiol. 2007 Apr;30(3):202-6
references.pmid: 16904280
references.citation: Appl Environ Microbiol. 2002 May;68(5):2214-28
references.pmid: 11976091
references.citation: J Mol Biol. 1990 Oct 5;215(3):403-10
references.pmid: 2231712
references.citation: Microbiol Rev. 1995 Mar;59(1):143-69
references.pmid: 7535888
references.citation: Appl Environ Microbiol. 1996 Feb;62(2):625-30
references.pmid: 8593063
references.citation: Environ Microbiol. 2005 Aug;7(8):1162-74
references.pmid: 16011753
references.citation: Annu Rev Microbiol. 2003;57:369-94
references.pmid: 14527284