Anti-inflammatory effect of crude extract and isolated compounds from Baccharis illinita DC in acute skin inflammation.
ETHNOPHARMACOLOGYCAL RELEVANCE: The tea from the leaves of Baccharis illinita DC (Asteraceae family) is commonly used by the population as anti-inflammatory (including topically), protective gastric and anti-infectious. However, no studies have been done with this species to confirm its topical anti-inflammatory action.


OBJECTIVE
This study evaluated he topical effects of crude extract of leaves (CE) and its active constituents in 12-O-tetradecanoylphorbol acetate (TPA)-induced ear oedema.


METHODS
CE and compounds effects were tested in commonly used models of TPA-, arachidonic acid (AA)- and capsaicin-ear oedema. Polymorphonuclear (PMN) cell migration was evaluated by mieloperoxidase and analyzed histologically.


RESULTS
CE (0.1-1 mg/ear) caused a dose-related inhibition of TPA-induced ear oedema and PMN influx similarly to that produced by topical application of the steroidal anti-inflammatory drug dexamethasone. The active constituents of the AcOEt fraction kaurenoic acid, alpha-spinasterol, oleanolic acid and baurenol also inhibited TPA-induced ear edema. Histological analysis of the ear of CE-treated animals confirmed the reduction of edema and of PMN infiltration. Both CE and the nosteroidal anti-inflammatory drug indomethacin inhibited the AA-induced ear oedema, but did not change capsaicin-induced oedema.


CONCLUSIONS
These results indicate that the CE and the active constituents have a topical anti-inflammatory effect and the possible mechanisms for the pharmacological effects are discussed.
Boller
Shirley
S
Department of Pharmacology, Federal University Federal of Paraná, Curitiba, PR, Brazil.
Soldi
Cristian
C
Marques
Maria C A
MC
Santos
Elide P
EP
Cabrini
Daniela A
DA
Pizzolatti
Moacir G
MG
Zampronio
Aleksander R
AR
Otuki
Michel F
MF
D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D016718:Arachidonic Acid; D031175:Baccharis; D002211:Capsaicin; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004423:Ear; D004487:Edema / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D008297:Male; D051379:Mice; D020556:Neutrophil Infiltration / Q000187:drug effects; D009828:Oleanolic Acid / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves; D028321:Plant Preparations / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D013265:Stigmasterol / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate
D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D028321:Plant Preparations; C031312:spinasterol; D016718:Arachidonic Acid; C051436:kaurenoic acid; D009828:Oleanolic Acid; D013265:Stigmasterol; D013755:Tetradecanoylphorbol Acetate; D002211:Capsaicin