title: Effect of phthalate esters on the secretion of prostaglandins (F2alpha and E2) and oxytocin in cultured bovine ovarian and endometrial cells.
abstract: The influence of phthalate esters di-2-ethylhexyl phthalate (DEHP) and mono-2-ethylhexyl phthalate (MEHP) on uterine prostaglandin (PGF2alpha and PGE2) and ovarian oxytocin secretion was investigated. Endometrial, granulosa, and luteal cells from cows on days 8-12 of the estrous cycle were treated with DEHP or MEHP (0.1, 1, or 10 ng/mL). We found that DEHP and MEHP stimulated (P < 0.05) secretion of PGF2alpha and inhibited (P < 0.001) secretion of PGE2 from endometrial cells. The ratio of PGF2alpha to PGE2 was markedly altered. The endocrine disrupting chemicals also enhanced secretion of oxytocin (P < 0.05) from ovarian cells. Our results indicated that DEHP and its metabolite MEHP could affect the process of the estrous cycle by impairing secretion of prostaglandin from the uterus and oxytocin from the ovary.
authors.affiliation: Department Gynecology and Obstetrics, Beijing Military Region Main Hospital, Dongcheng District, Beijing 100700, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Lixin
authors.initials: L
authors.lastname: Shang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.forename: Shuhe
authors.initials: S
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D015237:Dinoprost / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism; D052244:Endocrine Disruptors / Q000494:pharmacology*; D004717:Endometrium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004785:Environmental Pollutants / Q000494:pharmacology; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008184:Luteal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010121:Oxytocin / Q000187:drug effects / Q000378:metabolism; D018709:Statistics, Nonparametric
chemical_list: D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D010121:Oxytocin; D015237:Dinoprost; D004051:Diethylhexyl Phthalate; C016599:mono-(2-ethylhexyl)phthalate; D015232:Dinoprostone