title: Modelling the hair follicle dermal papilla using spheroid cell cultures.
abstract: Human dermal papilla (DP) cells grown in two-dimensional (2D) culture have been studied extensively. However, key differences exist between DP cell activities in vivo and in vitro. Using a suspension method of cell culture to maintain DP cells, we created three-dimensional (3D) dermal spheres morphologically akin to intact (anagen) DPs. Analysis of these spheres using immunocytochemistry demonstrates that they have expression profiles different from papilla cells cultured in 2D but with many similarities to intact DPs. This method of DP cell culture may provide us with a tool to elucidate our understanding of signalling within the DP as it relates to induction, maintenance or even inhibition of hair growth.
authors.forename: Claire A
authors.initials: CA
authors.lastname: Higgins
authors.forename: Gavin D
authors.initials: GD
authors.lastname: Richardson
authors.forename: Dudley
authors.initials: D
authors.lastname: Ferdinando
authors.forename: Gillian E
authors.initials: GE
authors.lastname: Westgate
authors.forename: Colin A B
authors.initials: CA
authors.lastname: Jahoda
mesh_terms: D000199:Actins / Q000378:metabolism; D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics; D000469:Alkaline Phosphatase / Q000378:metabolism; D060466:Axin Protein; D018929:Cell Culture Techniques / Q000379:methods*; D002467:Cell Nucleus / Q000378:metabolism; D018031:Connexin 43 / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics; D020405:Dermis / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics; D038362:Glycogen Synthase Kinase 3 / Q000235:genetics; D000071679:Glycogen Synthase Kinase 3 beta; D018859:Hair Follicle / Q000166:cytology*; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism; D006801:Humans; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000235:genetics; D008954:Models, Biological; D019006:Neural Cell Adhesion Molecules / Q000235:genetics; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D012697:Serine Endopeptidases / Q000235:genetics; D018874:Spheroids, Cellular / Q000166:cytology* / Q000378:metabolism; D053675:Versicans / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: C491761:AXIN2 protein, human; D000199:Actins; D025601:Adenomatous Polyposis Coli Protein; D060466:Axin Protein; C495270:CTNNB1 protein, human; D018031:Connexin 43; D003598:Cytoskeletal Proteins; C500216:GJA1 protein, human; D019812:Heparan Sulfate Proteoglycans; C497573:LEF1 protein, human; D051821:Lymphoid Enhancer-Binding Factor 1; D019006:Neural Cell Adhesion Molecules; D018809:Proliferating Cell Nuclear Antigen; C508322:VCAN protein, human; D051176:beta Catenin; D053675:Versicans; C071927:perlecan; D011451:Prostaglandin-Endoperoxide Synthases; D000071679:Glycogen Synthase Kinase 3 beta; D038362:Glycogen Synthase Kinase 3; D000469:Alkaline Phosphatase; C119812:CORIN protein, human; D012697:Serine Endopeptidases