title: Multiplex Amplicon Quantification (MAQ), a fast and efficient method for the simultaneous detection of copy number alterations in neuroblastoma.
abstract: BACKGROUND
  Cancer genomes display characteristic patterns of chromosomal imbalances, often with diagnostic and prognostic relevance. Therefore assays for genome-wide copy number screening and simultaneous detection of copy number alterations in specific chromosomal regions are of increasing importance in the diagnostic work-up of tumors.
  
  
  RESULTS
  We tested the performance of Multiplex Amplicon Quantification, a newly developed low-cost, closed-tube and high-throughput PCR-based technique for detection of copy number alterations in regions with prognostic relevance for neuroblastoma. Comparison with array CGH and the established Multiplex Ligation-dependent Probe Amplification method on 52 neuroblastoma tumors showed that Multiplex Amplicon Quantification can reliably detect the important genomic aberrations.
  
  
  CONCLUSIONS
  Multiplex Amplicon Quantification is a low-cost and high-throughput PCR-based technique that can reliably detect copy number alterations in regions with prognostic relevance for neuroblastoma.
authors.affiliation: Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium.
authors.forename: Candy
authors.initials: C
authors.lastname: Kumps
authors.forename: Nadine
authors.initials: N
authors.lastname: Van Roy
authors.forename: Lien
authors.initials: L
authors.lastname: Heyrman
authors.forename: Dirk
authors.initials: D
authors.lastname: Goossens
authors.forename: Jurgen
authors.initials: J
authors.lastname: Del-Favero
authors.forename: Rosa
authors.initials: R
authors.lastname: Noguera
authors.forename: Jo
authors.initials: J
authors.lastname: Vandesompele
authors.forename: Frank
authors.initials: F
authors.lastname: Speleman
authors.forename: Katleen
authors.initials: K
authors.lastname: De Preter
mesh_terms: D056915:DNA Copy Number Variations; D006801:Humans; D009447:Neuroblastoma / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods*; D016133:Polymerase Chain Reaction
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