Qualitative drug analysis of hair extracts by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry.
A technique using comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GCxGC/TOFMS) is applied to a qualitative analysis of three sample extracts from hair suspected of containing various drug compounds. The samples were also subjected to a quantitative target analysis for codeine, morphine, 6-monoacetylmorphine (6-MAM), amphetamine, methamphetamine, methylenedioxyamphetamine (MDA), methylenedioxymethylamphetamine (MDMA), methadone, and benzylpiperazine (BZP) by liquid chromatography-tandem mass spectrometry (LC-MS/MS). GCxGC/TOFMS provided a non-specific procedure that identified various drugs, metabolites, and impurities not included in the target analysis. They included cocaine, diazepam, and methaqualone (quaalude). Comprehensive GCxGC separation was achieved using twin-stage cryo-modulation to focus eluant from a DB-5 ms (5% phenyl) to a BPX50 (50% phenyl) GC column. The TOF mass spectrometer provided unit mass resolution in the mass range m/z 5-1000 and rapid spectral acquisition (< or = 500spectra/s). Clean mass spectra of the individual components were obtained using mass spectral deconvolution software. The 'unknown' components were identified by comparison with mass spectra stored in a library database.
Guthery
Bill
B
Planetary and Space Sciences Research Institute, The Open University, Walton Hall, Milton Keynes, MK7 6AA, UK. w.guthery@open.ac.uk
Bassindale
Tom
T
Bassindale
Alan
A
Pillinger
Colin T
CT
Morgan
Geraint H
GH
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis*
D004364:Pharmaceutical Preparations