title: Oligonucleotide-selenide conjugate: synthesis and its inducible sequence-specific alkylation of DNA.
abstract: Oligonucleotide-selenium conjugate was designed and synthesized and its sequence-specific cross-linking ability was investigated. The selenide derivatives can generate covalent interstrand cross-linking with its complementary strand through the formation of o-QM intermediate induced by periodate oxidation. A cross-linking reaction yield of up to 50% was obtained. Hydroxyl radical footprinting experiment revealed that the quinone appendage specifically alkylated the cytosine base extending the duplex formed between the conjugate and the target strand.
authors.affiliation: Wuhan University, Hubei, Wuhan 430072, People's Republic of China.
authors.forename: Yuhao
authors.initials: Y
authors.lastname: Du
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Weng
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.forename: Heng
authors.initials: H
authors.lastname: Ma
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Constant
mesh_terms: D000477:Alkylating Agents / Q000737:chemistry*; D000478:Alkylation; D001483:Base Sequence; D003432:Cross-Linking Reagents; D003596:Cytosine / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D012643:Selenium / Q000737:chemistry*
chemical_list: D000477:Alkylating Agents; D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D003596:Cytosine; D004247:DNA; D012643:Selenium