The silicon trypanosome.
African trypanosomes have emerged as promising unicellular model organisms for the next generation of systems biology. They offer unique advantages, due to their relative simplicity, the availability of all standard genomics techniques and a long history of quantitative research. Reproducible cultivation methods exist for morphologically and physiologically distinct life-cycle stages. The genome has been sequenced, and microarrays, RNA-interference and high-accuracy metabolomics are available. Furthermore, the availability of extensive kinetic data on all glycolytic enzymes has led to the early development of a complete, experiment-based dynamic model of an important biochemical pathway. Here we describe the achievements of trypanosome systems biology so far and outline the necessary steps towards the ambitious aim of creating a 'Silicon Trypanosome', a comprehensive, experiment-based, multi-scale mathematical model of trypanosome physiology. We expect that, in the long run, the quantitative modelling enabled by the Silicon Trypanosome will play a key role in selecting the most suitable targets for developing new anti-parasite drugs.
Bakker
Barbara M
BM
Department of Pediatrics, Center for Liver, Digestive and Metabolic Diseases, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands. b.m.bakker@med.umcg.nl
Krauth-Siegel
R Luise
RL
Clayton
Christine
C
Matthews
Keith
K
Girolami
Mark
M
Westerhoff
Hans V
HV
Michels
Paul A M
PA
Breitling
Rainer
R
Barrett
Michael P
MP
D010274:Parasitology / Q000379:methods* / Q000639:trends*; D049490:Systems Biology / Q000379:methods / Q000639:trends; D014345:Trypanosoma / Q000235:genetics* / Q000378:metabolism*