The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow.
Through the preparation of a novel controlled pore glass-poly(pyrrole) material we have developed a conducting support that is not only suitable for the co-immobilisation of enzymes and co-factors, but also enables the facile electrochemical regeneration of the co-factor during a reaction. Employing the selective reduction of (rac)-2-phenylpropionaldehyde to (S)-phenyl-1-propanol as a model, we have demonstrated the successful co-immobilisation of the HLADH enzyme and co-factor NAD(H); with incorporation of the material into a continuous flow reactor facilitating the in situ electrochemical regeneration of NAD(H) for in excess of 100 h. Using this approach we have developed a reagent-less, atom efficient system applicable to the cost-effective, continuous biosynthesis of chiral compounds.
Ngamsom
B
B
Department of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
Hickey
A M
AM
Greenway
G M
GM
Littlechild
J A
JA
McCreedy
T
T
Watts
P
P
Wiles
C
C
D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D055162:Biocatalysis; D004553:Electric Conductivity; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005898:Glass / Q000737:chemistry; D007700:Kinetics; D009243:NAD / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D011758:Pyrroles / Q000737:chemistry; D013816:Thermodynamics
D004800:Enzymes, Immobilized; D011108:Polymers; D011758:Pyrroles; D009243:NAD; C067635:polypyrrole; D000426:Alcohol Dehydrogenase