title: Surface elasticity imaging of vascular tissues in a liquid environment by a scanning haptic microscope.
abstract: The objective of this study was to make an elasticity distribution image of natural arteries in a liquid environment at high resolution at the micrometer level and at a wide area at the sub-square millimeter level by improving the scanning haptic microscope (SHM), developed previously for characterization of the stiffness of natural tissues. The circumferential sections (thickness, 1.0 mm) of small-caliber porcine arteries (approximately 3-mm diameter) were used as a sample. Measurement was performed by soaking a probe (diameter, 5 microm; spatial resolution, less than 2 microm) in saline solution at an appropriate depth. The vascular tissues were segregated by multi-layering a high elasticity region with mainly elastin (50.8 +/- 13.8 kPa) and a low one with mainly collagen and smooth muscle cells (17.0 +/- 9.0 kPa), as observed previously in high humidity conditions. The elasticity was measured repeatedly with little change for over 4 h in a liquid environment, which enabled observation with maintenance of high precision of a large area of at least 1,200 x 100 microm, whereas the elasticity was increased with time by the dehydration of samples with shrinkage in the air, in which an averaged elasticity in the overall area was approximately doubled within 2 h. This simple, inexpensive system allows observation of the distribution of the surface elasticity at the extracellular matrix level of vascular tissues in a liquid environment close to the natural one.
authors.affiliation: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka, 565-8565, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Oie
authors.forename: Hisato
authors.initials: H
authors.lastname: Suzuki
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Murayama
authors.forename: Toru
authors.initials: T
authors.lastname: Fukuda
authors.forename: Sadao
authors.initials: S
authors.lastname: Omata
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kanda
authors.forename: Keiichi
authors.initials: K
authors.lastname: Takamizawa
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Nakayama
mesh_terms: D000818:Animals; D001158:Arteries / Q000502:physiology*; D004548:Elasticity; D005109:Extracellular Matrix / Q000502:physiology*; D008853:Microscopy; D013552:Swine
references.citation: Innovations (Phila). 2009 Nov;4(6):345-50
references.pmid: 22437233
references.citation: Biophys J. 2004 May;86(5):3269-83
references.pmid: 15111440
references.citation: J Artif Organs. 2007;10(1):10-5
references.pmid: 17380291
references.citation: Biophys J. 2006 Aug 15;91(4):1521-31
references.pmid: 16731557
references.citation: J Thorac Cardiovasc Surg. 2007 Jul;134(1):152-9
references.pmid: 17599501
references.citation: J Artif Organs. 2009;12(1):40-6
references.pmid: 19330504
references.citation: J Biomed Mater Res B Appl Biomater. 2010 Jan;92(1):236-42
references.pmid: 19921747
references.citation: Med Eng Phys. 2007 Apr;29(3):307-15
references.pmid: 16750415
references.citation: J Biomed Mater Res A. 2006 Dec 1;79(3):485-94
references.pmid: 16779854
references.citation: IEEE Trans Med Imaging. 2008 Sep;27(9):1275-87
references.pmid: 18753043
references.citation: J Artif Organs. 2010 Jul;13(2):106-12
references.pmid: 20213453
references.citation: J Biomech. 2004 Jan;37(1):67-72
references.pmid: 14672569
references.citation: Appl Environ Microbiol. 2005 Feb;71(2):955-60
references.pmid: 15691953
references.citation: Phys Rev Lett. 1986 Mar 3;56(9):930-933
references.pmid: 10033323
references.citation: Circulation. 2010 Sep 14;122(11 Suppl):S100-6
references.pmid: 20837900
references.citation: Med Eng Phys. 2008 Mar;30(2):206-12
references.pmid: 17383214