title: Isolation and expression analyses of the Sox9a gene in triploid crucian carp.
abstract: To investigate the evolutional significance of Sox9 in fish, we isolated and characterized Sox9a cDNA and genomic clones in triploid crucian carp. The cDNA encoded a protein of 457 amino acids with an HMG box and showed more than 60% amino acid sequence identity with known vertebrate Sox9 proteins. Triploid crucian carp and vertebrate Sox9s showed similar gene structure, and two introns in the coding region were located at conserved positions. On the basis of the amino acid sequences, Sox9a can be categorized into the same subgroup of Sox-E proteins as Sox8, 9, and 10. Interestingly, the expression of triploid crucian carp Sox9a was predominantly observed not in the ovary but in the testis by Northern blot and RT-PCR analysis. The expression analysis of Sox9a suggested that it may seldom contribute to the formation of normal functions of spermatozoa, but it may play an important role in the development of testicular tubules. Besides the testicular expression, Sox9a was also shown to be expressed in many other tissues including the brain, kidney, and heart of triploid crucian carp, indicating that Sox9 may have unique functions in some specific tissues during development.
authors.affiliation: College of Life Sciences, Hunan Normal University, ChangSha 410081, Hunan, P.R. China.
authors.forename: Xinhong
authors.initials: X
authors.lastname: Guo
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Yan
authors.forename: Shaojun
authors.initials: S
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Xiang
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics* / Q000254:growth & development; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D029941:Fish Proteins / Q000235:genetics*; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011123:Polyploidy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055758:SOX9 Transcription Factor / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D029941:Fish Proteins; D055758:SOX9 Transcription Factor
references.citation: Development. 1996 Sep;122(9):2813-22
references.pmid: 8787755
references.citation: Yi Chuan Xue Bao. 2003 May;30(5):414-8
references.pmid: 12924154
references.citation: Science. 1998 Nov 27;282(5394):1711-4
references.pmid: 9831563
references.citation: Genesis. 2002 Jul;33(3):131-9
references.pmid: 12124946
references.citation: J Exp Zool. 1999 Nov 1;284(6):705-10
references.pmid: 10531557
references.citation: Nat Genet. 1998 Apr;18(4):345-9
references.pmid: 9537416
references.citation: Dev Biol. 2001 Mar 1;231(1):149-63
references.pmid: 11180959
references.citation: Nat Genet. 1996 Sep;14(1):62-8
references.pmid: 8782821
references.citation: J Biol Chem. 1997 Oct 31;272(44):27848-52
references.pmid: 9346931
references.citation: Mol Reprod Dev. 2003 Nov;66(3):211-7
references.pmid: 14502599
references.citation: Cell. 1994 Dec 16;79(6):1111-20
references.pmid: 8001137
references.citation: Am J Med Genet. 1983 May;15(1):3-28
references.pmid: 6344634
references.citation: J Genet. 2007 Aug;86(2):85-91
references.pmid: 17968136
references.citation: Nature. 1994 Dec 8;372(6506):525-30
references.pmid: 7990924
references.citation: Brain Res. 2001 Jul 6;906(1-2):143-8
references.pmid: 11430871
references.citation: Dev Dyn. 2003 Nov;228(3):480-9
references.pmid: 14579386
references.citation: Biochem Biophys Res Commun. 2005 Aug 5;333(3):729-36
references.pmid: 15963466
references.citation: Mol Reprod Dev. 2002 Sep;63(1):5-16
references.pmid: 12211055
references.citation: Development. 2004 May;131(9):1891-901
references.pmid: 15056615
references.citation: Nat Genet. 2001 Jul;28(3):216-7
references.pmid: 11431689
references.citation: DNA Cell Biol. 1997 Aug;16(8):1003-11
references.pmid: 9303442
references.citation: Gene. 1997 Nov 20;202(1-2):167-70
references.pmid: 9427561