Isolation and characterization of bacteriophages infecting Staphylococcus epidermidis.
Bacteriophages infecting Staphylococcus epidermidis were isolated by mitomycin C induction. Three distinct phages (vB_SepiS-phiIPLA5, vB_SepiS-phiIPLA6, and vB_SepiS-phiIPLA7)-defined by plaque morphology, structure, virion proteins pattern, DNA restriction bands, and host range-were obtained. One-step growth curves of bacteriophages under optimal growth conditions for S. epidermidis F12 revealed eclipse and latent periods of 5-10 and 10-15 min, respectively, with burst sizes of about 5 to 30 PFU per infected cell. Transmission electron microscopy revealed that the phages were of similar size and belonged to the Siphoviridae family. Phage phi-IPLA7 had the broadest host range infecting 21 out of 65 S. epidermidis isolates. Phage phi-IPLA5 seemed to be a virulent phage probably derived from phi-IPLA6. Phages phi-IPLA5 and phi-IPLA7 exhibited increasing plaques surrounded by a halo that could be indicative of a polysaccharide depolymerase activity. Viable counts, determined during the infection of S. epidermidis F12, confirmed that phi-IPLA5 had a potent lytic capability and reduced S. epidermidis population by 5.67 log units in 8 h of incubation; in the presence of the mixture of phi-IPLA6 and phi-IPLA7, however, a reduction of 2.27 log units was detected.
Gutiérrez
Diana
D
Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Apdo. 85, 33300, Villaviciosa, Asturias, Spain.
Martínez
Beatriz
B
Rodríguez
Ana
A
García
Pilar
P
D015169:Colony Count, Microbial; D016172:DNA Fingerprinting; D058507:Host Specificity; D006801:Humans; D050296:Microbial Viability; D046529:Microscopy, Electron, Transmission; D016685:Mitomycin / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D017906:Siphoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D013204:Staphylococcus Phages / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000821:virology*; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000032:analysis; D014775:Virus Activation / Q000187:drug effects
D014764:Viral Proteins; D016685:Mitomycin
J Pharm Pharmacol. 2008 Dec;60(12):1551-71
19000360
Antimicrob Agents Chemother. 2006 Apr;50(4):1268-75
16569839
Nature. 1970 Aug 15;227(5259):680-5
5432063
J Bacteriol. 2005 Apr;187(7):2426-38
15774886
PLoS One. 2009;4(3):e4944
19300511
Clin Otolaryngol. 2009 Aug;34(4):349-57
19673983
Curr Top Microbiol Immunol. 2008;322:207-28
18453278
J Bacteriol. 2007 Mar;189(5):2086-100
17172342
PLoS Pathog. 2009 Jan;5(1):e1000253
19119417
J Appl Microbiol. 1998 Sep;85(3):583-90
9750288
J Bacteriol. 1993 Dec;175(23):7541-9
8244923
Vet Microbiol. 2009 Feb 16;134(1-2):45-54
18986783
Nat Rev Microbiol. 2009 Aug;7(8):555-67
19609257
Arch Virol. 2003 Jun;148(6):1051-70
12756614
Mol Microbiol. 2004 Feb;51(4):1169-83
14763988
J Clin Microbiol. 1976 May;3(5):519-23
132457
BMC Microbiol. 2009 May 07;9:82
19422689
Vet Microbiol. 2007 Sep 20;124(1-2):187-91
17509779
J Dairy Sci. 2009 Jul;92(7):3019-26
19528579