title: A case of generalized bovine tuberculosis in a sheep.
abstract: The present report describes a rare case of generalized bovine-type tuberculosis in a slaughtered 4-year-old ewe discovered during routine surveillance at an abattoir. A postmortem examination revealed lesions in the ewe's thoracic and abdominal cavities, ranging from encapsulated, mineralized foci to extensive, soft, caseous tissue. Lesions in the lungs, liver, and lymph nodes were consistent with mycobacterial infection. A histopathological examination detected granulomatous lesions in all tissue samples. The presence of Mycobacterium tuberculosis complex genome was confirmed through a polymerase chain reaction (PCR) analysis of tissues, using IS6110 primers, followed by a nucleotide sequence analysis of PCR products. Acid-fast bacteria, characterized as Mycobacterium bovis, were isolated from lesions following 38 days of incubation.
authors.affiliation: Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy. cinzia.marianelli@iss.it
authors.forename: Cinzia
authors.initials: C
authors.lastname: Marianelli
authors.forename: Noemi
authors.initials: N
authors.lastname: Cifani
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Capucchio
authors.forename: Michele
authors.initials: M
authors.lastname: Fiasconaro
authors.forename: Miriam
authors.initials: M
authors.lastname: Russo
authors.forename: Francesco
authors.initials: F
authors.lastname: La Mancusa
authors.forename: Paolo
authors.initials: P
authors.lastname: Pasquali
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Di Marco
mesh_terms: D000003:Abattoirs; D000818:Animals; D002417:Cattle; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D016680:Genome, Bacterial; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000473:pathology / Q000635:transmission
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial