Crystallization and preliminary structural characterization of the two actin-depolymerization factors of the malaria parasite.
The malaria parasite Plasmodium depends on its actin-based motor system for motility and host-cell invasion. Actin-depolymerization factors are important regulatory proteins that affect the rate of actin turnover. Plasmodium has two actin-depolymerization factors which seem to have different functions and display low sequence homology to the higher eukaryotic family members. Plasmodium actin-depolymerization factors 1 and 2 have been crystallized. The crystals diffracted X-rays to maximum resolutions of 2.0 and 2.1 A and belonged to space groups P3(1)21 or P3(2)21, with unit-cell parameters a = b = 68.8, c = 76.0 A, and P2(1)2(1)2, with unit-cell parameters a = 111.6, b = 57.9, c = 40.5 A, respectively, indicating the presence of one or two molecules per asymmetric unit in both cases.
Huttu
Jani
J
Centre for Structural Systems Biology, Helmholtz Centre for Infection Research and University of Hamburg, DESY, 22607 Hamburg, Germany.
Singh
Bishal Kumar
BK
Bhargav
Saligram Prabhakar
SP
Sattler
Julia M
JM
Schüler
Herwig
H
Kursula
Inari
I
D018360:Crystallography, X-Ray; D008958:Models, Molecular; D010962:Plasmodium berghei / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000737:chemistry*; D015800:Protozoan Proteins / Q000737:chemistry*
D015800:Protozoan Proteins
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