Oxidative stress in the ischemic and non-ischemic parts of the rat liver after two-thirds ischemia/reperfusion.
Rat liver was subjected to two-thirds warm ischemia for 45 min and reperfusion (I/R) to evaluate the resulting oxidative stress. The plasma alanine aminotransferase and aspartate aminotransferase activities were significantly higher than those in the sham group 1.5-24 h after I/R, showing extensive liver cell death. The level of oxidative stress was compared between the ischemic and non-ischemic regions based on the change in antioxidative vitamins C and E. The vitamin C level was significantly decreased during I/R in both the ischemic and non-ischemic regions 0, 1.5, 3, 6, 12, and 24 h after the start of reperfusion, showing enhanced oxidative stress even in the non-ischemic lobules. This decrease of vitamin C in the ischemic region was significantly higher than that in the non-ischemic lobules, while the vitamin E content was decreased only in the ischemic lobes, demonstrating higher oxidative stress in the ischemic region than that in the non-ischemic region. Early transient activation of cytoprotective extracellular signal-related kinase (ERK) was apparent in both the ischemic and non-ischemic lobules, reflecting oxidative stress in both regions. Early transient activation of c-Jun NH(2)-terminal kinase (JNK) was only apparent in the ischemic region, corresponding to extensive oxidative stress and liver cell death. These results demonstrate that significant oxidative stress was induced, but that JNK leading to cell death was not activated in the non-ischemic part of the liver.
Kitamura
Yuko
Y
Department of Food Science and Nutrition, Nara Women's University, Nara, Japan.
Washino
Yukiko
Y
Koga
Eriko
E
Ito
Ayaka
A
Kawagoe
Maiko
M
Nakazaki
Chihiro
C
Kiso
Kaori
K
Ichi
Ikuyo
I
Matsura
Tatsuya
T
Kojo
Shosuke
S
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000097:blood; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000098:blood supply* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology*; D014810:Vitamin E / Q000097:blood / Q000378:metabolism
D014810:Vitamin E; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D020928:Mitogen-Activated Protein Kinases; D001205:Ascorbic Acid