Effect of St. John's Wort (Hypericum perforatum) treatment on restraint stress-induced behavioral and biochemical alteration in mice.
BACKGROUND
A stressful stimulus is a crucial determinant of health and disease. Antidepressants are used to manage stress and their related effects. The present study was designed to investigate the effect of St. John's Wort (Hypericum perforatum) in restraint stress-induced behavioral and biochemical alterations in mice.


METHODS
Animals were immobilized for a period of 6 hr. St. John's Wort (50 and 100 mg/kg) was administered 30 minutes before the animals were subjecting to acute immobilized stress. Various behavioral tests parameters for anxiety, locomotor activity and nociceptive threshold were assessed followed by biochemical assessments (malondialdehyde level, glutathione, catalase, nitrite and protein) subsequently.


RESULTS
6-hr acute restraint stress caused severe anxiety like behavior, antinociception and impaired locomotor activity as compared to unstressed animals. Biochemical analyses revealed an increase in malondialdehyde, nitrites concentration, depletion of reduced glutathione and catalase activity as compared to unstressed animal brain. Five days St. John's Wort treatment in a dose of 50 mg/kg and 100 mg/kg significantly attenuated restraint stress-induced behavioral (improved locomotor activity, reduced tail flick latency and antianxiety like effect) and oxidative damage as compared to control (restraint stress).


CONCLUSIONS
Present study highlights the modest activity of St. John's Wort against acute restraint stress induced modification.
Kumar
Anil
A
Pharmacology Division, UGC Centre of Advance Study, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh-160014, India. kumaruips@yahoo.com
Garg
Ruchika
R
Prakash
Atish K
AK
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001007:Anxiety / Q000188:drug therapy* / Q000378:metabolism; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D020902:Hypericum; D008124:Locomotion / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D009573:Nitrites / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D012149:Restraint, Physical / Q000523:psychology; D013315:Stress, Psychological / Q000188:drug therapy* / Q000378:metabolism
D000975:Antioxidants; D009573:Nitrites; D010936:Plant Extracts; D008315:Malondialdehyde; D002374:Catalase; D005978:Glutathione
Ann N Y Acad Sci. 1999 Jun 29;877:281-91
10415655
J Psychiatry Neurosci. 2005 May;30(3):196-201
15944744
Anal Biochem. 1982 Oct;126(1):131-8
7181105
Trends Pharmacol Sci. 2001 Jun;22(6):292-7
11395157
FASEB J. 1996 Nov;10(13):1532-8
8940299
Eur J Pharmacol. 2003 Feb 28;463(1-3):235-72
12600714
Planta Med. 2007 Oct;73(13):1358-64
17918039
Pharmacopsychiatry. 1998 Jun;31 Suppl 1:54-9
9684948
Life Sci. 1978 Sep 18;23(11):1143-51
713690
Ann N Y Acad Sci. 2004 Dec;1032:1-7
15677391
Biochem J. 1966 Jun;99(3):667-76
5964963
Eur J Pharmacol. 2003 Jan 1;458(1-2):135-9
12498917
Eur J Pharmacol. 2004 Jun 16;493(1-3):111-5
15189771
Neuropsychopharmacology. 2009 Mar;34(4):923-31
18496519
J Affect Disord. 2006 Mar;91(1):63-70
16430969
Arch Biochem Biophys. 1959 May;82(1):70-7
13650640
Psychopharmacology (Berl). 2001 Feb;153(4):402-14
11243487
Acta Physiol Hung. 1992;80(1-4):117-25
1345179
Neuroreport. 1992 Oct;3(10):849-52
1421086
Neuropharmacology. 2001 Jun;40(8):959-75
11406187
Pharmacol Rep. 2007 May-Jun;59(3):284-90
17652828
Int Clin Psychopharmacol. 2004 Mar;19(2):89-95
15076017
Med Sci Monit. 2003 Feb;9(2):RA23-34
12601303
Eur J Neurosci. 2005 Sep;22(5):1190-200
16176362
Int J Biochem. 1994 Apr;26(4):511-7
8013736
Indian J Exp Biol. 2008 Mar;46(3):159-63
18432054
Endocr Rev. 1991 May;12(2):118-34
2070776
J Pharm Pharmacol. 1999 May;51(5):513-7
10411209
Braz J Med Biol Res. 2001 Feb;34(2):241-4
11175500
Eur J Pharmacol. 2007 Oct 15;572(1):23-31
17610875
Brain Res. 1998 Jul 20;799(2):215-29
9675286
Arch Intern Med. 2000 Jan 24;160(2):152-6
10647752
Methods Find Exp Clin Pharmacol. 2006 Mar;28(2):95-9
16636719
Physiol Behav. 2007 Mar 16;90(4):626-8
17196624
Biol Psychiatry. 1998 Dec 15;44(12):1229-38
9861466
Life Sci. 1997;60(26):PL403-13
9199489
Eur Neuropsychopharmacol. 2000 Sep;10(5):407-13
10974614
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Eur J Pharmacol. 2006 Mar 27;535(1-3):192-8
16522321