title: General route toward side-chain-functionalized alpha-helical polypeptides.
abstract: A series of poly(gamma-chloropropyl-L-glutamate) (PCPLG) with controlled polymer molecular weight (MW = 5-28 kg x mol(-1)) and molecular weight distribution (PDI = 1.16-1.26) have been prepared from hexamethyldisilazane (HMDS)-mediated ring-opening polymerization (ROP) of gamma-chloropropyl-L-glutamic acid based N-carboxylanhydride (CP-NCA). CD, FTIR, and WAXS analysis reveal that the polymers adopt alpha-helical conformations both in solution and the solid state. Their helical surfaces can be readily decorated with functional moieties to confer desired properties, as demonstrated by the quantitative derivatization of the PCPLG side chains with azido functional groups that are amendable to "click" chemistry. Subsequent side-chain conjugation with mannose moieties via copper-mediated [2 + 3] alkyne-azide 1,3-dipolar cycloaddition affords water-soluble mannose-polypeptide conjugates with quantitative grafting efficiency occurring under mild conditions. CD analysis reveals that the mannose-polypeptide conjugates also retain alpha-helical conformations in aqueous solution.
authors.affiliation: Department of Chemistry and Macromolecular Studies Group, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
authors.forename: Haoyu
authors.initials: H
authors.lastname: Tang
authors.forename: Donghui
authors.initials: D
authors.lastname: Zhang
mesh_terms: D002942:Circular Dichroism; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000737:chemistry*; D008970:Molecular Weight; D017646:Organosilicon Compounds / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D011487:Protein Conformation; D012996:Solutions
chemical_list: D017646:Organosilicon Compounds; D010455:Peptides; D012996:Solutions; C553273:poly(gamma-azidopropylglutamate); C553272:poly(gamma-chloropropylglutamate); D011099:Polyglutamic Acid; C024548:hexamethylsilazane; D008358:Mannose