[Effects of acrylamide on the protein expressions of c-fos and c-jun proto-oncogenes in cell L-02].
OBJECTIVE
To investigate the cell apoptosis and expressions of c-fos and c-jun proteins induced by acrylamide (AA) in L-02 human liver embryo cell.


METHODS
The cell survival rate, cell apoptosis and protein expressions of c-fos and c-jun were measured respectively after L-02 cell were exposed to different final concentration of AA (low: 0.01, 0.1 mmol/l; middle: 0.1, 0.5, 2.5 mmol/l; high: 5.0, 7.5 mmol/l) for 12 h and 24 h, respectively.


RESULTS
The cell survival rate in the low dose AA-treated group was higher than that in the control group, but the high dose AA-treated groups demonstrated a significantly lower cell survival rate than the control group (P < 0.05). The percentages of apoptosis were significantly higher than that of the control group in the high dose AA-treated groups (P < 0.05). The expressions of c-fos and c-jun of AA-treated group were higher than that in the control group (P < 0.05), and the expressions increased first and then decreased with the AA exaltation; furthermore, the expressions also raised along with the exprosure time (P < 0.05).


CONCLUSIONS
AA can induce the apoptosis of L-02 cell and high protein expressions of c-fos and c-jun proto-oncogenes.
Wang
Rui
R
Shenzhen Center for Chronic Disease Control, Shenzhen 518020, China. zhenjin.1983@163.com
Liu
Jianjun
J
Huang
Cai
C
Qian
Qifeng
Q
Li
Qing
Q
Liu
Xiaoli
X
D020106:Acrylamide / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D005333:Fetus; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism*
D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D020106:Acrylamide