title: HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP.
abstract: HIV-1 transactivator Tat has greatly contributed to our understanding of transcription elongation by RNAPII. We purified HIV-1 Tat-associated factors from HeLa nuclear extract and show that Tat forms two distinct and stable complexes. Tatcom1 consists of the core active P-TEFb, MLL-fusion partners involved in leukemia (AF9, AFF4, AFF1, ENL, and ELL), and PAF1 complex. Importantly, Tatcom1 formation relies on P-TEFb while optimal CDK9 CTD-kinase activity is AF9 dependent. MLL-fusion partners and PAF1 are required for Tat transactivation. Tatcom2 is composed of CDK9, CycT1, and 7SK snRNP lacking HEXIM. Tat remodels 7SK snRNP by interacting directly with 7SK RNA, leading to the formation of a stress-resistant 7SK snRNP particle. Besides the identification of factors required for Tat transactivation and important for P-TEFb function, our data show a coordinated control of RNAPII elongation by different classes of transcription elongation factors associated in a single complex and acting at the same promoter.
authors.affiliation: Laboratoire de Virologie Moléculaire, Institut de Génétique Humaine, CNRS-UPR1142, Montpellier, France. bijan.sobhian@igh.cnrs.fr
authors.forename: Bijan
authors.initials: B
authors.lastname: Sobhian
authors.forename: Nadine
authors.initials: N
authors.lastname: Laguette
authors.forename: Ahmad
authors.initials: A
authors.lastname: Yatim
authors.forename: Mirai
authors.initials: M
authors.lastname: Nakamura
authors.forename: Yves
authors.initials: Y
authors.lastname: Levy
authors.forename: Rosemary
authors.initials: R
authors.lastname: Kiernan
authors.forename: Monsef
authors.initials: M
authors.lastname: Benkirane
mesh_terms: D001665:Binding Sites; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D042863:Cyclin-Dependent Kinase 9 / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D046912:Multiprotein Complexes; D051788:Myeloid-Lymphoid Leukemia Protein / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D015514:Oncogene Proteins, Fusion / Q000378:metabolism; D042925:Positive Transcriptional Elongation Factor B / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000096:biosynthesis*; D012097:Repressor Proteins / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D014157:Transcription Factors; D015533:Transcriptional Activation; D035602:Transcriptional Elongation Factors / Q000378:metabolism; D014162:Transfection; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism*
chemical_list: C471348:AFF4 protein, human; D004268:DNA-Binding Proteins; C090862:ELL protein, human; C497422:KMT2A protein, human; C489901:MLLT3 protein, human; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D015514:Oncogene Proteins, Fusion; C501972:PAF1 protein, human; D012367:RNA, Viral; D012097:Repressor Proteins; D017411:Ribonucleoproteins, Small Nuclear; D014157:Transcription Factors; D035602:Transcriptional Elongation Factors; D054322:tat Gene Products, Human Immunodeficiency Virus; D051788:Myeloid-Lymphoid Leukemia Protein; C081449:AFF1 protein, human; D011495:Histone-Lysine N-Methyltransferase; D042925:Positive Transcriptional Elongation Factor B; C467969:CDK9 protein, human; D042863:Cyclin-Dependent Kinase 9
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