title: Helicobacter hepaticus Hh0072 gene encodes a novel alpha1-3-fucosyltransferase belonging to CAZy GT11 family.
abstract: Lewis x (Le(x)) and sialyl Lewis x (SLe(x))-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is alpha1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus alpha1-3-fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Le(x), sialyl Le(x) and their derivatives. Unlike all other alpha1-3/4-fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with alpha1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first alpha1-3-fucosyltransferase identified in the GT11 family.
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Kam
authors.initials: K
authors.lastname: Lau
authors.forename: Jiansong
authors.initials: J
authors.lastname: Cheng
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Li
authors.forename: Go
authors.initials: G
authors.lastname: Sugiarto
authors.forename: Shengshu
authors.initials: S
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.forename: Vireak
authors.initials: V
authors.lastname: Thon
authors.forename: Peng G
authors.initials: PG
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D005647:Fucosyltransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D044923:Helicobacter hepaticus / Q000235:genetics*; D008954:Models, Biological; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D016695:Glycosyltransferases; D005647:Fucosyltransferases
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