title: Crystallization and preliminary X-ray analysis of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Staphylococcus aureus.
abstract: 6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the Mg(2+)-dependent transfer of pyrophosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (HMDP), forming 6-hydroxymethyl-7,8-dihydropterin pyrophosphate, which is a critical step in the de novo folic acid-biosynthesis pathway. Diffraction-quality crystals of HPPK from the medically relevant species Staphylococcus aureus were grown in the presence of ammonium sulfate or sodium malonate and diffracted to better than 1.65 A resolution. The crystals belonged to space group P2(1), with unit-cell parameters a = 36.8, b = 76.6, c = 51.5 A, alpha = gamma = 90.0, beta = 100.2 degrees . The crystals contained two molecules per asymmetric unit, with a volume per protein weight (V(M)) of 2.04 A(3) Da(-1) and an estimated solvent content of 39.6%.
authors.affiliation: Medicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, Victoria 3052, Australia.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Chhabra
authors.forename: Janet
authors.initials: J
authors.lastname: Newman
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Peat
authors.forename: Ross T
authors.initials: RT
authors.lastname: Fernley
authors.forename: Joanne
authors.initials: J
authors.lastname: Caine
authors.forename: Jamie S
authors.initials: JS
authors.lastname: Simpson
authors.forename: James D
authors.initials: JD
authors.lastname: Swarbrick
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D017854:Diphosphotransferases / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: D017854:Diphosphotransferases; C019684:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
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