Differences in AMPK expression between subcutaneous and visceral adipose tissue in morbid obesity.
Adenine monophosphate (AMP) activated protein kinase (AMPK) is an important regulator of obesity. The objective of the present work was to study and compare AMPK protein expression in visceral vs. subcutaneous adipose tissue of morbid obese subjects and to correlate it with adipose tissue characteristics. We selected a total population of 17 extreme obese (BMI>or=40 kg/m2) aged 42.8+/-10.2 years were included in this study. We measured anthropometric and body composition parameters. Adiponectin expression by qRT-PCR, isoproterenol-stimulated lipolytic rates, and AMPK alpha subunits expression by Western blot in adipose tissue explants were determined. Finally plasma concentrations of glucose, triacylglycerols, total cholesterol, HDL-c, LDL-c and insulin were also measured. Our results showed that AMPK alpha expression was higher in subcutaneous than in visceral tissue. A positive correlation between AMPK expression and adiponectin expression in human subcutaneous adipose tissue was observed. Furthermore, a positive correlation between AMPK expression and isoproterenol evoked upregulation of lipolysis rate was also observed. In conclusion, AMPK alpha expression differed according to adipose tissue location. The positive correlation between subcutaneous adipose tissue AMPK and adiponectin or the evoked lipolysis rate could indicate a protective role of AMPK in this tissue, counteracting insulin resistance in morbid obese patients.
Martínez-Agustin
O
O
Department of Biochemistry and Molecular Biology II, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBER-EHD), School of Pharmacy, University of Granada, Spain.
Hernández-Morante
J J
JJ
Martínez-Plata
E
E
Sánchez de Medina
F
F
Garaulet
M
M
D055372:AMP-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D000273:Adipose Tissue / Q000201:enzymology* / Q000378:metabolism; D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D009767:Obesity, Morbid / Q000201:enzymology* / Q000378:metabolism
D055372:AMP-Activated Protein Kinases