Complete mtDNA genomes of Anopheles darlingi and an approach to anopheline divergence time.
BACKGROUND
The complete sequences of the mitochondrial genomes (mtDNA) of members of the northern and southern genotypes of Anopheles (Nyssorhynchus) darlingi were used for comparative studies to estimate the time to the most recent common ancestor for modern anophelines, to evaluate differentiation within this taxon, and to seek evidence of incipient speciation.


METHODS
The mtDNAs were sequenced from mosquitoes from Belize and Brazil and comparative analyses of structure and base composition, among others, were performed. A maximum likelihood approach linked with phylogenetic information was employed to detect evidence of selection and a Bayesian approach was used to date the split between the subgenus Nyssorhynchus and other Anopheles subgenera.


RESULTS
The comparison of mtDNA sequences within the Anopheles darlingi taxon does not provide sufficient resolution to establish different units of speciation within the species. In addition, no evidence of positive selection in any protein-coding gene of the mtDNA was detected, and purifying selection likely is the basis for this lack of diversity. Bayesian analysis supports the conclusion that the most recent ancestor of Nyssorhynchus and Anopheles+Cellia was extant ~94 million years ago.


CONCLUSIONS
Analyses of mtDNA genomes of Anopheles darlingi do not provide support for speciation in the taxon. The dates estimated for divergence among the anopheline groups tested is in agreement with the geological split of western Gondwana (95 mya), and provides additional support for explaining the absence of Cellia in the New World, and Nyssorhynchus in the Afro-Eurasian continents.
Moreno
Marta
M
Griffin Laboratory, New York State Department of Health, Wadsworth Center, 5668 State Farm Road, Slingerlands, NY 12159, USA. mxm40@health.state.ny.us
Marinotti
Osvaldo
O
Krzywinski
Jaroslaw
J
Tadei
Wanderli P
WP
James
Anthony A
AA
Achee
Nicole L
NL
Conn
Jan E
JE
D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics*; D001482:Base Composition / Q000235:genetics*; D001499:Bayes Theorem; D001531:Belize; D001938:Brazil; D004272:DNA, Mitochondrial / Q000145:classification / Q000235:genetics*; D005260:Female; D017344:Genes, Insect; D049810:Genetic Speciation; D054629:Genome, Mitochondrial / Q000235:genetics*; D005838:Genotype; D008390:Markov Chains; D009010:Monte Carlo Method; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013997:Time Factors
D004272:DNA, Mitochondrial
BMC Ecol. 2008 Mar 26;8:3
18366795
Trends Genet. 2007 Jun;23(6):259-63
17418445
J Am Mosq Control Assoc. 1990 Mar;6(1):7-34
2324726
PLoS Biol. 2005 Sep;3(9):e285
16076241
PLoS Biol. 2006 May;4(5):e88
16683862
Gene. 1999 Sep 30;238(1):195-209
10570997
Insect Mol Biol. 1993;2(2):103-24
9087549
Heredity (Edinb). 2006 Apr;96(4):311-21
16508661
Mol Biol Evol. 1986 Sep;3(5):418-26
3444411
Mol Phylogenet Evol. 2006 May;39(2):417-23
16473530
Trends Parasitol. 2003 May;19(5):214-9
12763427
PLoS One. 2009;4(2):e4549
19234606
Nature. 1981 Apr 9;290(5806):470-4
7219536
Methods Mol Biol. 2000;132:365-86
10547847
Mol Phylogenet Evol. 2007 Apr;43(1):283-97
17126567
Nature. 1995 Jul 13;376(6536):163-5
7603565
J Mol Evol. 1995 Sep;41(3):353-8
7563121
Genome. 2005 Feb;48(1):46-54
15729396
J Mol Evol. 2007 May;64(5):519-27
17460806
Mol Biol Evol. 2002 Jun;19(6):950-8
12032251
J Mol Evol. 1985;22(3):252-71
3001325
J Med Entomol. 2008 Jul;45(4):667-76
18714866
BMC Genomics. 2005 Aug 09;6:105
16091132
Nucleic Acids Res. 1997 Mar 1;25(5):955-64
9023104
Mol Biol Evol. 2002 Jun;19(6):908-17
12032247
Bioinformatics. 2003 Dec 12;19(18):2496-7
14668244
Annu Rev Entomol. 2003;48:111-39
12208816
Methods Enzymol. 2005;395:311-48
15865975
Brief Bioinform. 2004 Jun;5(2):150-63
15260895
Mem Inst Oswaldo Cruz. 1996 Jul-Aug;91(4):391-8
9070397
BMC Evol Biol. 2007 Nov 08;7:214
17996036
Genome. 1993 Dec;36(6):1058-73
8112570
BMC Evol Biol. 2007 Aug 30;7:154
17760998
Nat Rev Genet. 2004 Feb;5(2):114-22
14735122
Bioessays. 2005 Oct;27(10):1069-75
16163732
Mol Biol Evol. 2005 Apr;22(4):1107-18
15689528
Adv Genet. 1999;41:1-33
10494615
Bioinformatics. 1998;14(9):817-8
9918953
Am J Trop Med Hyg. 1999 Mar;60(3):364-76
10466962
Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6491-5
8022810
Insect Mol Biol. 2002 Dec;11(6):605-10
12421418
Infect Genet Evol. 2008 Sep;8(5):737-46
18640289
BMC Evol Biol. 2009 Dec 22;9:298
20028549
Insect Mol Biol. 1996 Feb;5(1):51-9
8630535
Comput Appl Biosci. 1997 Oct;13(5):555-6
9367129
Am J Trop Med Hyg. 2006 May;74(5):798-806
16687683
Philos Trans R Soc Lond B Biol Sci. 1998 Feb 28;353(1366):287-305
9533126
Mol Phylogenet Evol. 2009 Sep;52(3):588-601
19603555
Gene. 2004 Sep 15;339:7-15
15363841
Bioinformatics. 2003 Aug 12;19(12):1572-4
12912839
Trends Parasitol. 2003 Mar;19(3):144-9
12643998
Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):6994-7001
10860962
Trends Genet. 2006 Feb;22(2):79-83
16356585
Mol Biol Evol. 2005 Dec;22(12):2472-9
16107592
Science. 2006 Apr 28;312(5773):570-2
16645093
Genetics. 1987 Jun;116(2):215-23
3038671
Genetics. 2005 Dec;171(4):1695-705
16118189
Heredity (Edinb). 2007 Sep;99(3):245-56
17534382
Nucleic Acids Res. 2003 Jul 1;31(13):3672-8
12824391
Mol Biol Evol. 2002 May;19(5):748-61
11961108
J Med Entomol. 2006 Sep;43(5):947-56
17017232
J Hered. 2001 Jul-Aug;92(4):371-3
11535656
J Med Entomol. 1999 Sep;36(5):631-4
10534960
Mol Biol Evol. 2007 Sep;24(9):2132-8
17636041