High-yield production of short chain length poly(epsilon-L-lysine) consisting of 5-20 residues by Streptomyces aureofaciens, and its antimicrobial activity.
Poly(epsilon-L-lysine) (epsilon-PL) is a naturally-occurring L-lysine homopolymer having antimicrobial activity. A newly-isolated strain of Streptomyces aureofaciens produced a short chain length epsilon-PL consisting of 5-20 residues at the highest production level of 4.5 g l(-1). This epsilon-PL had different spectra in terms of antimicrobial activity from the epsilon-PL that is now used as a food preservative. The high productivity was based on multiple metabolic pathways for L-lysine synthesis, and a great flux from L-lysine to epsilon-PL. The usefulness of this new epsilon-PL and its producing strain was discussed.
Takehara
Munenori
M
Department of Materials Science, University of Shiga Prefecture, Hassaka, Hikone 522-8533, Japan. takehara@mat.usp.ac.jp
Hibino
Atsushi
A
Saimura
Masayuki
M
Hirohara
Hideo
H
D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008954:Models, Biological; D011107:Polylysine / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D013304:Streptomyces aureofaciens / Q000378:metabolism*
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D011107:Polylysine