title: Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O12.
abstract: The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O12. Its structure was studied by sugar analysis using GLC of the alditol acetates and (S)-2-octyl glycosides, methylation analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy, including 2D (1)H-(1)H COSY, TOCSY, ROESY, (1)H-(13)C HSQC, and HMBC experiments. It was found that the polymer is a neutral heteropolysaccharide and has a branched heptasaccharide repeating unit with the following structure:
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation. lina_chuk@mail.ru
authors.forename: Angelina A
authors.initials: AA
authors.lastname: Parkhomchuk
authors.forename: Nina A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: Magdalena
authors.initials: M
authors.lastname: Białczak-Kokot
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Alexander O
authors.initials: AO
authors.lastname: Chizhov
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Antoni
authors.initials: A
authors.lastname: Rozalski
mesh_terms: D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011532:Providencia / Q000737:chemistry*
chemical_list: D019081:O Antigens