title: Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of macrophage growth locus A (MglA) protein from Francisella tularensis.
abstract: Francisella tularensis, a potential bioweapon, causes a rare infectious disease called tularemia in humans and animals. The macrophage growth locus A (MglA) protein from F. tularensis associates with RNA polymerase to positively regulate the expression of multiple virulence factors that are required for its survival and replication within macrophages. The MglA protein was overproduced in Escherichia coli, purified and crystallized. The crystals diffracted to 7.5 A resolution at the Advanced Photon Source, Argonne National Laboratory and belonged to the hexagonal space group P6(1) or P6(5), with unit-cell parameters a = b = 125, c = 54 A.
authors.affiliation: Macromolecular Crystallography Laboratory, National Cancer Institute, 1050 Boyles Street, Frederick, MD 21702, USA.
authors.forename: Priadarsini
authors.initials: P
authors.lastname: Subburaman
authors.forename: Brian P
authors.initials: BP
authors.lastname: Austin
authors.forename: Gary X
authors.initials: GX
authors.lastname: Shaw
authors.forename: David S
authors.initials: DS
authors.lastname: Waugh
authors.forename: Xinhua
authors.initials: X
authors.lastname: Ji
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D005604:Francisella tularensis / Q000737:chemistry*; D015870:Gene Expression
chemical_list: D001426:Bacterial Proteins
references.citation: J Bacteriol. 2007 Sep;189(18):6580-6
references.pmid: 17644593
references.citation: Clin Microbiol Rev. 2002 Oct;15(4):631-46
references.pmid: 12364373
references.citation: Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):398-406
references.pmid: 11856824
references.citation: J Biol Chem. 2005 Apr 29;280(17):17380-91
references.pmid: 15735307
references.citation: Protein Expr Purif. 2006 Jan;45(1):175-82
references.pmid: 16168669
references.citation: PLoS Pathog. 2007 Jun;3(6):e84
references.pmid: 17571921
references.citation: JAMA. 2001 Jun 6;285(21):2763-73
references.pmid: 11386933
references.citation: Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4246-9
references.pmid: 15010524
references.citation: FEMS Microbiol Lett. 2002 Sep 24;215(1):53-6
references.pmid: 12393200
references.citation: J Mol Biol. 1968 Apr 28;33(2):491-7
references.pmid: 5700707