Prevention of Candida albicans biofilm formation by covalently bound dimethylaminoethylmethacrylate and polyethylenimine.
Candida albicans biofilms are a major cause of voice prosthesis deterioration in laryngectomized patients. The aim of this study was to produce a surface capable of inhibiting C. albicans biofilm formation. Dimethylaminoethylmethacrylate (DMAEMA) and polyethylenimine (PEI) moieties were covalently bound to the surface of polydimethylsiloxane (PDMS) or polymethylmethacrylate (PMMA) and subsequently quaternized. Physicochemical characterization of the grafted surfaces was carried out and their effect on C. albicans cell numbers was assessed using a modified Robbins device to grow the biofilms. Covalently bound quaternized polyDMAEMA (polyDMAEMAq) and PEI (PEIq) inhibited biofilm growth, with reductions up to 92%. Our approach may show promise for future application in medical devices such as catheters and prostheses.
De Prijck
Kristof
K
Laboratory of Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium.
De Smet
Nele
N
Coenye
Tom
T
Schacht
Etienne
E
Nelis
Hans J
HJ
D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development*; D015169:Colony Count, Microbial / Q000379:methods; D004129:Dimethylpolysiloxanes / Q000378:metabolism; D004864:Equipment and Supplies / Q000382:microbiology; D005021:Ethylamines / Q000494:pharmacology*; D006801:Humans; D008689:Methacrylates / Q000494:pharmacology*; D011094:Polyethyleneimine / Q000494:pharmacology*; D019904:Polymethyl Methacrylate / Q000378:metabolism
C510335:2-dimethylaminoethyl methacrylate; D000935:Antifungal Agents; D004129:Dimethylpolysiloxanes; D005021:Ethylamines; D008689:Methacrylates; C013830:baysilon; D011094:Polyethyleneimine; D019904:Polymethyl Methacrylate
Laryngoscope. 2002 Apr;112(4):708-12
12152601
Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17667-71
17101983
Mycopathologia. 2010 Mar;169(3):167-74
19774486
J Biomed Mater Res B Appl Biomater. 2007 May;81(2):358-70
17022068
Biotechnol Prog. 2006 Mar-Apr;22(2):584-9
16599580
Appl Environ Microbiol. 2006 May;72(5):3673-7
16672516
J Appl Microbiol. 2008 Sep;105(3):733-40
18341554
Biofouling. 2010;26(3):269-75
20054722
Biotechnol Bioeng. 2005 Jun 20;90(6):715-22
15803464
Biotechnol Lett. 2008 Mar;30(3):475-9
17972018
J Biomed Mater Res. 2001;58(5):556-63
11505431
Biotechnol Bioeng. 2003 Jul 20;83(2):168-72
12768622
Biomaterials. 2006 Oct;27(30):5151-60
16797692
Trends Biotechnol. 2005 Jul;23(7):343-8
15922467
Biomacromolecules. 2007 May;8(5):1359-84
17425365
Otolaryngol Clin North Am. 2004 Jun;37(3):655-77
15163608
Acta Otolaryngol. 1999;119(5):604-8
10478604
Biomaterials. 2002 Mar;23(6):1417-23
11829437
Arch Otolaryngol Head Neck Surg. 2000 Nov;126(11):1320-8
11074828
Ann Otol Rhinol Laryngol. 2002 Mar;111(3 Pt 1):200-3
11915880
J Biomater Sci Polym Ed. 2009;20(14):2039-53
19874676
Proc Natl Acad Sci U S A. 2001 May 22;98(11):5981-5
11353851
Biotechnol Bioeng. 2002 Aug 20;79(4):465-71
12115410
Appl Environ Microbiol. 2004 Jul;70(7):4408-10
15240331
Dent Mater J. 2005 Dec;24(4):570-82
16445020