Down-regulated genes in mouse dental papillae and pulp.
Important factors involved in odontogenesis in mouse dental papillae disappear between the pre- and post-natal stages of development. Therefore, we hypothesized that certain genes involved in odontogenesis in dental papillae were subject to pre-/post-natal down-regulation. Our goal was to identify, by microarray analysis, which genes were down-regulated. Dental papillae were isolated from embryonic 16-day-, 18-day- (E16, E18), and post-natal 3-day-old (P3) murine first mandibular molar germs and analyzed by microarray. The number of down-regulated genes was 2269 between E16 and E18, and 3130 between E18 and P3. Drastic down-regulation (fold change > 10.0) of Adamts4, Aldha1a2, and Lef1 was observed at both E16 and E18, and quantitative RT-PCR revealed a post-natal reduction in their expression (Adamts4, 1/3; Aldh1a2, 1/13; and Lef1, 1/37). These results suggest that down-regulation of these three genes is an important factor in normal odontogenesis in dental papillae.
Sasaki
H
H
Oral Health Science Center HRC7, Tokyo Dental College, Japan.
Muramatsu
T
T
Kwon
H-J
HJ
Yamamoto
H
H
Hashimoto
S
S
Jung
H-S
HS
Shimono
M
M
D051722:ADAM Proteins / Q000032:analysis / Q000235:genetics; D000071121:ADAMTS4 Protein; D000444:Aldehyde Dehydrogenase / Q000032:analysis / Q000235:genetics; D000080924:Aldehyde Dehydrogenase 1 Family; D000818:Animals; D016923:Cell Death / Q000235:genetics; D003771:Dental Papilla / Q000166:cytology* / Q000196:embryology; D003782:Dental Pulp / Q000166:cytology* / Q000196:embryology; D015536:Down-Regulation / Q000235:genetics*; D004847:Epithelial Cells / Q000166:cytology; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D017403:In Situ Hybridization; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000032:analysis / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D009804:Odontoblasts / Q000166:cytology; D009805:Odontogenesis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011348:Procollagen N-Endopeptidase / Q000032:analysis / Q000235:genetics; D050697:Retinal Dehydrogenase; D014074:Tooth Calcification / Q000235:genetics; D014083:Tooth Germ / Q000166:cytology / Q000196:embryology
C497574:Lef1 protein, mouse; D051821:Lymphoid Enhancer-Binding Factor 1; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; C512722:Aldh1a2 protein, mouse; D050697:Retinal Dehydrogenase; D051722:ADAM Proteins; D011348:Procollagen N-Endopeptidase; D000071121:ADAMTS4 Protein; C000603919:Adamts4 protein, mouse