Cloning, expression, and induction by 17-beta estradiol (E2) of a vitellogenin gene in the white cloud mountain minnow Tanichthys albonubes.
Vitellogenins (Vtgs), the precursors for the yolk proteins, are very important for the embryonic development of teleosts, and have also been studied extensively as biomarkers for environmental estrogenic mimics. The cDNA for a Vtg was isolated from the liver of the female white cloud mountain minnow (Tanichthys albonubes) by 3'- and 5'-RACE methods. It is 4,171 bp in full length, and encodes a putative protein of 1,326 amino acids. This putative Vtg, designated as wcmmVtg, contains complete portions of LVI and PV, but lacks the C-terminal half of LVII and thus belongs to type I vitellogenin. In addition to the liver of the female fish, wcmmVtg was also shown to be expressed in the ovary. During ovarian development, the mRNA expression of wcmmVtg in both the liver and ovary was continuously increased from the previtellogenic to late vitellogenic stages, but then decreased significantly at post-spawning stage. In the male fish, expression of wcmmVtg mRNA was induced in the liver by treatment with E2 (10 and 100 ng/l) for 14 days. These results suggest that the Vtg originated from the ovary of the white cloud mountain minnow may also contribute to the accumulation of yolk proteins during oocyte growth, and that the male white cloud mountain minnow is sensitive to the estrogenic treatment in terms of Vtg mRNA expression, which could also be applied to monitor the environmental estrogenic mimics.
Wang
Ruilong
R
Institute of Aquatic Economic Animals, School of Life Sciences, Zhongshan (Sun Yat-Sen) University, Guangzhou 510275, People's Republic of China.
Gao
Yun
Y
Zhang
Lihong
L
Zhang
Yike
Y
Fang
Zhanqiang
Z
He
Jianguo
J
Zhang
Weimin
W
Ma
Guangzhi
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D029941:Fish Proteins / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014819:Vitellogenins / Q000235:genetics*
D017931:DNA Primers; D029941:Fish Proteins; D012333:RNA, Messenger; D014819:Vitellogenins; D004958:Estradiol
Biol Reprod. 2004 Dec;71(6):2056-64
15329331
Environ Health Perspect. 1996 Oct;104(10):1096-101
8930552
Trends Genet. 1988 Aug;4(8):227-32
3072724
Dev Biol (N Y 1985). 1985;1:127-77
3917200
Nat Toxins. 1999;7(2):71-9
10495469
Comp Biochem Physiol B Biochem Mol Biol. 2006 Apr;143(4):404-14
16460978
Biol Reprod. 2003 Jul;69(1):355-64
12672675
J Mol Evol. 1995 Jul;41(1):104-12
7608984
Comp Biochem Physiol B Biochem Mol Biol. 2007 Dec;148(4):445-53
17804271
Gen Comp Endocrinol. 2000 Dec;120(3):300-13
11121295
J Steroid Biochem Mol Biol. 2006 Aug;100(4-5):193-201
16854581
Gene. 2005 Aug 15;356:91-100
15979250
Mol Reprod Dev. 2007 Jun;74(6):665-73
17075797
Environ Health Perspect. 1999 May;107(5):385-90
10210694
Mol Gen Genet. 2000 Jun;263(5):828-37
10905350
Gene. 2003 Jan 16;303:99-109
12559571
Gene. 2004 Mar 17;328:25-36
15019981
Nat Toxins. 1998;6(1):35-41
9851510
J Exp Zool A Comp Exp Biol. 2003 Feb 1;295(2):117-26
12541295