title: Enhancement of nerve regeneration along a chitosan nanofiber mesh tube on which electrically polarized beta-tricalcium phosphate particles are immobilized.
abstract: The ability of beta-tricalcium phosphate (beta-TCP) particles to store electric charge was confirmed by thermally stimulated depolarization current measurement as well as surface potential measurement. The efficacy of stored electrical charge on beta-TCP particles in enhancing nerve regeneration was evaluated. Bridge grafting was performed into sciatic nerve defects in Wistar rats with the following tubes: chitosan mesh tubes; chitosan mesh tubes on which beta-TCP particles with or without electrical polarization treatment had been immobilized (polarized and non-polarized tubes, respectively). As a control, isografts were used. Both motor and sensory nerve function as well as electrophysiological recovery progressed with time in each group. Immunofluorescence revealed rapider nerve regeneration in the polarized tube group compared with the non-polarized tube group. The axon density and axon area in the polarized tube group were significantly greater than those in the chitosan mesh tube and non-polarized group, and showed no significant differences from the control group. These results suggest that the stored charge on electrically polarized beta-TCP particles immobilized on chitosan mesh tubes may enhance nerve regeneration to the same extent as isografting.
authors.affiliation: Department of Inorganic Materials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda, Tokyo, Japan.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Soichiro
authors.initials: S
authors.lastname: Itoh
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Okawa
authors.forename: Akiko
authors.initials: A
authors.lastname: Nagai
authors.forename: Kimihiro
authors.initials: K
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry* / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D055664:Electrochemical Techniques; D008297:Male; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry*; D009416:Nerve Regeneration / Q000502:physiology*; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000473:pathology / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D048271:Chitosan