Helicobacter hepaticus Hh0072 gene encodes a novel alpha1-3-fucosyltransferase belonging to CAZy GT11 family.
Lewis x (Le(x)) and sialyl Lewis x (SLe(x))-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is alpha1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus alpha1-3-fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Le(x), sialyl Le(x) and their derivatives. Unlike all other alpha1-3/4-fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with alpha1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first alpha1-3-fucosyltransferase identified in the GT11 family.
Zhang
Lei
L
Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.
Lau
Kam
K
Cheng
Jiansong
J
Yu
Hai
H
Li
Yanhong
Y
Sugiarto
Go
G
Huang
Shengshu
S
Ding
Li
L
Thon
Vireak
V
Wang
Peng G
PG
Chen
Xi
X
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D005647:Fucosyltransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D044923:Helicobacter hepaticus / Q000235:genetics*; D008954:Models, Biological; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D016695:Glycosyltransferases; D005647:Fucosyltransferases
FEBS Lett. 2003 Oct 9;553(1-2):99-103
14550554
J Am Chem Soc. 2005 Dec 21;127(50):17618-9
16351087
FEMS Immunol Med Microbiol. 1996 Dec 1;16(2):105-15
8988391
Curr Opin Cell Biol. 2003 Oct;15(5):531-8
14519387
Mol Microbiol. 2000 Jun;36(6):1187-96
10931272
Bioinformatics. 2007 Oct 1;23(19):2536-42
17150993
Biochem J. 1997 Sep 15;326 ( Pt 3):929-39
9334165
Microbiology (Reading). 1999 Nov;145 ( Pt 11):3245-3253
10589734
Glycobiology. 1995 Oct;5(7):683-8
8608270
Glycobiology. 2008 Apr;18(4):290-302
18252778
Protein Sci. 1999 Aug;8(8):1668-74
10452611
Nat Rev Drug Discov. 2005 Jun;4(6):477-88
15931257
Infect Immun. 1997 Aug;65(8):3126-31
9234764
J Biol Chem. 1995 Mar 3;270(9):4632-9
7876234
Glycoconj J. 2004;20(5):353-64
15229399
Glycobiology. 2005 Nov;15(11):1076-83
16000696
J Mol Biol. 2003 Apr 25;328(2):307-17
12691742
Glycobiology. 2000 Jun;10(6):611-21
10814703
Curr Opin Struct Biol. 2002 Oct;12(5):599-608
12464311
J Biol Chem. 2003 Jun 13;278(24):21893-900
12676935
Am J Physiol Gastrointest Liver Physiol. 2004 Mar;286(3):G361-6
14766534
Biochem J. 1994 Jun 15;300 ( Pt 3):623-6
8010942
Genet Mol Res. 2008 Jul 29;7(3):663-78
18752194
J Biol Chem. 2007 Mar 30;282(13):9973-9982
17251184
J Biol Chem. 1996 Apr 19;271(16):9830-7
8621666
Angew Chem Int Ed Engl. 2000 Mar;39(5):836-863
10760879
Chem Rev. 1998 Apr 2;98(2):833-862
11848916
Glycobiology. 2007 May;17(5):455-66
17172260
Nucleic Acids Res. 2009 Jan;37(Database issue):D233-8
18838391
Appl Environ Microbiol. 2005 Dec;71(12):7995-8001
16332778
J Biol Chem. 1995 Mar 3;270(9):4640-9
7876235
Arch Biochem Biophys. 2004 Jun 15;426(2):122-31
15158662
J Biochem. 2008 Jun;143(6):725-9
18218651
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16096-101
19805264
J Biol Chem. 2001 Jun 29;276(26):23748-56
11323419
FEMS Microbiol Lett. 2000 Aug 15;189(2):281-4
10930752
J Biol Chem. 1997 Aug 22;272(34):21357-63
9261149
Mol Biochem Parasitol. 1998 Jun 1;93(2):237-50
9662708
Biochemistry. 2001 May 8;40(18):5382-91
11331001
Nature. 2007 Apr 26;446(7139):1046-51
17460666
Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4207-12
19251666
Microbes Infect. 2007 Nov-Dec;9(14-15):1553-60
18024122
J Clin Microbiol. 1994 May;32(5):1238-45
8051250
Glycobiology. 2006 Feb;16(2):29R-37R
16037492
Biochemistry. 2006 Jul 4;45(26):8108-16
16800635
Expert Rev Vaccines. 2005 Oct;4(5):677-85
16221069
Eur J Biochem. 2001 Feb;268(4):1006-19
11179967
N Engl J Med. 2002 Oct 10;347(15):1175-86
12374879
Mol Biol Evol. 2000 Mar;17(3):337-51
10723735
J Biol Chem. 1998 May 8;273(19):11533-43
9565568
Acta Biochim Pol. 2007;54(3):537-49
17762900
Proc Natl Acad Sci U S A. 1990 Sep;87(17):6674-8
2118655
Bioorg Med Chem. 2004 Dec 15;12(24):6427-35
15556760
Mol Biol Evol. 2001 Nov;18(11):2083-91
11606704
Glycobiology. 2006 Dec;16(12):158R-184R
16973733
Biochemistry. 2008 Jan 8;47(1):378-87
18078329
Carbohydr Res. 2008 Aug 11;343(12):1952-65
18279843
J Biol Chem. 2005 Nov 4;280(44):36848-56
16150700
J Biol Chem. 2001 Sep 7;276(36):33952-63
11423539
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7901-6
12810954
Org Lett. 2007 Mar 29;9(7):1227-30
17338534
Eur J Biochem. 1997 Jun 15;246(3):750-5
9219535
J Am Chem Soc. 2009 Dec 30;131(51):18467-77
19947630
Org Lett. 2004 Jul 8;6(14):2345-8
15228275
Carbohydr Res. 1997 Dec;305(3-4):501-9
9648267
J Biochem. 2008 Jul;144(1):1-6
18272537
ACS Chem Biol. 2008 Sep 19;3(9):567-76
18729452
Biochim Biophys Acta. 2000 Sep 27;1487(2-3):275-85
11018479