title: Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver.
abstract: The aim of the present study was to determine the effects of a 6-month dietary vitamin E (VE) deficiency on DNA methylation and gene expression in rat liver. Two enzymes, 5-α-steroid reductase type 1 (SRD5A1) and the regulatory subunit of γ-glutamylcysteinyl synthetase (GCLM), which are differentially expressed on the mRNA level, were analysed for promoter methylation in putative cytosine-phospho-guanine (CpG) island regions located at the 5' end using base-specific cleavage and matrix-assisted laser desorption ionisation time-of-flight MS. A twofold increase in the mRNA level of SRD5A1 gene and a twofold decrease in the mRNA level of GCLM gene in VE-deficient animals were not associated with different CpG methylation of the analysed promoter region. Furthermore, global DNA methylation was not significantly different in these two groups. Thus, the present results indicate that the VE-induced regulation of SRD5A1 and GCLM in rat liver is not directly mediated by changes in promoter DNA methylation.
authors.affiliation: Molecular Prevention Group, Institute of Human Nutrition and Food Science, Christian-Albrechts-University of Kiel, Heinrich-Hecht-Platz 10, 24118 Kiel, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Fischer
authors.forename: Sonja
authors.initials: S
authors.lastname: Gaedicke
authors.forename: Jan
authors.initials: J
authors.lastname: Frank
authors.forename: Frank
authors.initials: F
authors.lastname: Döring
authors.forename: Gerald
authors.initials: G
authors.lastname: Rimbach
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D004032:Diet; D015971:Gene Expression Regulation, Enzymologic; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D014810:Vitamin E / Q000008:administration & dosage*; D014811:Vitamin E Deficiency / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D014810:Vitamin E; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; C548472:Srd5a1 protein, rat; D005721:Glutamate-Cysteine Ligase