title: Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain.
abstract: A technique developed to determine naphthalene dioxygenase (NDO) activity was optimized and used to study the biotransformation of indole to indigo by Pseudomonas sp. J26 whole cells. The maximum production of indigo was achieved at 25 degrees C using 2.5 mM indole when J26 was grown in the complex medium JPP, while indole concentrations higher than 4 mM proved toxic for cells. The maximum rate of indigo production was 0.56 nmol min(-1) mg dry biomass(-1), obtaining 75.5 microM of indigo after 8 h of incubation, while a maximal concentration (138.1 microM) of indigo was obtained after 20 h.
authors.affiliation: Planta Piloto de Procesos Industriales Microbiológicos PROIMI-CONICET Av Belgrano y Caseros T4001MVB Tucumán, Argentina.
authors.forename: Juan Pablo Riva
authors.initials: JP
authors.lastname: Mercadal
authors.forename: Paula
authors.initials: P
authors.lastname: Isaac
authors.forename: Faustino
authors.initials: F
authors.lastname: Siñeriz
authors.forename: Marcela Alejandra
authors.initials: MA
authors.lastname: Ferrero
mesh_terms: D001118:Argentina; D003470:Culture Media / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D007203:Indigo Carmine; D007211:Indoles / Q000378:metabolism*; D009281:Naphthalenes / Q000378:metabolism*; D011549:Pseudomonas / Q000302:isolation & purification* / Q000378:metabolism*; D013696:Temperature
chemical_list: D003470:Culture Media; D007211:Indoles; D009281:Naphthalenes; C031721:naphthalene; C030374:indole; D007203:Indigo Carmine