title: Pilot-scale production of fatty acid ethyl esters by an engineered Escherichia coli strain harboring the p(Microdiesel) plasmid.
abstract: Fatty acid ethyl esters (FAEEs) were produced in this study by the use of an engineered Escherichia coli p(Microdiesel) strain. Four fed-batch pilot scale cultivations were carried out by first using glycerol as sole carbon source for biomass production before glucose and oleic acid were added as carbon sources. Cultivations yielded a cell density of up to 61 +/- 3.1 g of cell dry mass (CDM) per liter and a maximal FAEE content of 25.4% +/- 1.1% (wt/wt) of CDM.
authors.affiliation: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, D-48149 Munster, Germany.
authors.forename: Yasser
authors.initials: Y
authors.lastname: Elbahloul
authors.forename: Alexander
authors.initials: A
authors.lastname: Steinbüchel
mesh_terms: D001674:Bioelectric Energy Sources; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004951:Esterification; D004952:Esters / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism; D010865:Pilot Projects; D010957:Plasmids / Q000235:genetics*
chemical_list: D003470:Culture Media; D004952:Esters; D005227:Fatty Acids; D019301:Oleic Acid; D005947:Glucose; D005990:Glycerol
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