A Microfluidic Platform for Characterization of Protein-Protein Interactions.
Traditionally, expensive and time consuming techniques such as mass spectrometry and Western Blotting have been used for characterization of protein-protein interactions. In this paper, we describe the design, fabrication, and testing of a rapid and inexpensive sensor, involving the use of microelectrodes in a microchannel, which can be used for real-time electrical detection of specific interactions between proteins. We have successfully demonstrated detection of target glycoprotein-glycoprotein interactions, antigen-antibody interactions, and glycoprotein-antigen interactions. We have also demonstrated the ability of this technique to distinguish between strong and weak interactions. Using this approach, it may be possible to multiplex an array of these sensors onto a chip and probe a complex mixture for various types of interactions involving protein molecules.
Javanmard
Mehdi
M
M. Javanmard and F. Pease are with the Department of Electrical Engineering, Stanford University, Stanford, CA 94305 USA ( mehdij@stanford.edu ; pease@cis.stanford.edu ). A. H. Talasaz, M. Nemat-Gorgani, D. E. Huber, and R. W. Davis are with the Stanford Genome Technology Center, Palo Alto, CA 94304 USA ( atalasaz@stanford.edu ; mohsenn@stanford.edu ; david.huber@stanford.edu ; dbowe@stanford.edu ). M. Ronaghi is with Illumina Inc., San Diego, CA 92121 USA ( mronaghi@illumina.com ).
Talasaz
Amirali H
AH
Nemat-Gorgani
Mohsen
M
Huber
David E
DE
Pease
Fabian
F
Ronaghi
Mostafa
M
Davis
Ronald W
RW
Nat Genet. 2002 Dec;32 Suppl:526-32
12454649
J Colloid Interface Sci. 2006 Jan 15;293(2):500-4
16102778
Lab Chip. 2009 May 21;9(10):1429-34
19417910
Electrophoresis. 2000 Jan;21(1):27-40
10634468
Biosens Bioelectron. 2005 Jan 15;20(7):1320-6
15590285
Biosens Bioelectron. 2004 May 15;19(10):1139-47
15046744
J Colloid Interface Sci. 2007 Jun 1;310(1):74-82
17346726
Anal Biochem. 1981 Apr;112(2):195-203
6266278
Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):820-4
12552089
Biomicrofluidics. 2007 Nov 30;1(4):44103
19693402
Lab Chip. 2005 Oct;5(10):1155-60
16175273
Lab Chip. 2007 May;7(5):603-10
17476379
Biomicrofluidics. 2007 May 10;1(2):21503
19693376
Lab Chip. 2001 Sep;1(1):76-82
15100895
Biosens Bioelectron. 2003 Dec 30;19(5):495-502
14623474
J Colloid Interface Sci. 2002 Feb 15;246(2):223-6
16290405