title: Generating knockout rats by transposon mutagenesis in spermatogonial stem cells.
abstract: Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat.
authors.affiliation: Max Delbrück Center for Molecular Medicine, Berlin, Germany. zivics@mdc-berlin.de
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Izsvák
authors.forename: Janine
authors.initials: J
authors.lastname: Fröhlich
authors.forename: Ivana
authors.initials: I
authors.lastname: Grabundzija
authors.forename: James R
authors.initials: JR
authors.lastname: Shirley
authors.forename: Heather M
authors.initials: HM
authors.lastname: Powell
authors.forename: Karen M
authors.initials: KM
authors.lastname: Chapman
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Ivics
authors.forename: F Kent
authors.initials: FK
authors.lastname: Hamra
mesh_terms: D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D055786:Gene Knockout Techniques / Q000379:methods*; D008297:Male; D016296:Mutagenesis; D051381:Rats / Q000235:genetics*; D013093:Spermatogonia / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D004251:DNA Transposable Elements
references.citation: Biol Reprod. 2002 Apr;66(4):944-9
references.pmid: 11906912
references.citation: Mamm Genome. 2007 May;18(5):338-46
references.pmid: 17557177
references.citation: Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14931-6
references.pmid: 12391306
references.citation: Nat Methods. 2009 Jun;6(6):415-22
references.pmid: 19478801
references.citation: Nat Rev Genet. 2005 Jun;6(6):507-12
references.pmid: 15931173
references.citation: Nat Methods. 2007 Feb;4(2):131-3
references.pmid: 17220894
references.citation: Genome Biol. 2007;8 Suppl 1:S1
references.pmid: 18047686
references.citation: Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9191-6
references.pmid: 11481482
references.citation: Proc Natl Acad Sci U S A. 2005 Nov 29;102(48):17430-5
references.pmid: 16293688
references.citation: Genesis. 2001 Jun;30(2):82-8
references.pmid: 11416868
references.citation: Cell. 2008 Dec 26;135(7):1287-98
references.pmid: 19109897
references.citation: PLoS One. 2009 Jul 21;4(7):e6308
references.pmid: 19621088
references.citation: Cell. 1997 Nov 14;91(4):501-10
references.pmid: 9390559
references.citation: Proc Natl Acad Sci U S A. 2005 Oct 4;102(40):14302-7
references.pmid: 16183739
references.citation: Cell. 2008 Dec 26;135(7):1299-310
references.pmid: 19109898