title: [Impact of TGF-beta1 antisense on collagen-binding integrins in keloid].
abstract: BACKGROUND
  The treatment of keloids remains challenging due to sparse knowledge about the pathogenesis of this disease. Transforming growth factor (TGF)-beta1 plays a central role in keloid formation. Cell-matrix communication is controlled by integrins, the expression of which can be regulated by TGF-beta1.
  
  
  METHODS
  Using immunohistochemistry we compared expression patterns of alpha1beta1, alpha2beta1 und alpha3beta1 in normal skin and keloid tissue. Secondly, the effect of TGF-beta1-antisense after 48 h and 72 h incubation in a keloid-derived fibroblast monolayer was analyzed by means of reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry.
  
  
  RESULTS
  alpha1beta1 and alpha2beta1 were highly expressed in keloid fibroblasts. Incubation with TGF-beta1-antisense lead to a reduction on protein level. RT-PCR demonstrated an increase of all alpha subunits, while on an mRNA level a decrease of the subunit beta1 could be observed.
  
  
  CONCLUSIONS
  Integrin expression is directly modulated by TGF-beta1. An abnormal response in the keloid as a result of an altered TGF-beta1 pathway could be a key element to understanding the development of keloids.
authors.affiliation: Universitäts-HNO-Klinik, Universitätsklinikum Mannheim, Universität Heidelberg, Deutschland. gregor.bran@umm.de
authors.forename: G
authors.initials: G
authors.lastname: Bran
authors.forename: U
authors.initials: U
authors.lastname: Sommer
authors.forename: F
authors.initials: F
authors.lastname: Meinzer
authors.forename: U R
authors.initials: UR
authors.lastname: Goessler
authors.forename: K
authors.initials: K
authors.lastname: Hörmann
authors.forename: F
authors.initials: F
authors.lastname: Riedel
authors.forename: H
authors.initials: H
authors.lastname: Sadick
mesh_terms: D003094:Collagen / Q000378:metabolism*; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D016023:Integrins / Q000378:metabolism*; D007627:Keloid / Q000473:pathology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D016023:Integrins; D016376:Oligonucleotides, Antisense; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D003094:Collagen
references.citation: Plast Reconstr Surg. 1996 Oct;98(5):827-33
references.pmid: 8823022
references.citation: J Cell Biol. 1995 Dec;131(6 Pt 2):1903-15
references.pmid: 8557756
references.citation: Exp Dermatol. 2002 Aug;11(4):311-8
references.pmid: 12190939
references.citation: Ann Plast Surg. 1999 Aug;43(2):179-84
references.pmid: 10454326
references.citation: Hautarzt. 2002 Aug;53(8):515-23
references.pmid: 12221465
references.citation: J Invest Dermatol. 1992 Nov;99(5):650-5
references.pmid: 1431230
references.citation: Cell Biochem Funct. 1998 Jun;16(2):129-37
references.pmid: 9637001
references.citation: Arch Surg. 1999 Nov;134(11):1209-15
references.pmid: 10555635
references.citation: Matrix Biol. 2000 Aug;19(4):325-32
references.pmid: 10963993
references.citation: Curr Rheumatol Rep. 1999 Oct;1(1):28-33
references.pmid: 11123011
references.citation: Mol Med Today. 1998 Jan;4(1):19-24
references.pmid: 9494966
references.citation: J Biol Chem. 1989 Dec 25;264(36):21806-11
references.pmid: 2600089
references.citation: Plast Reconstr Surg. 1999 Oct;104(5):1435-58
references.pmid: 10513931
references.citation: Hautarzt. 2007 Nov;58(11):979-89; quiz 990
references.pmid: 17928977
references.citation: Plast Reconstr Surg. 2001 Aug;108(2):423-9
references.pmid: 11496185
references.citation: J Biol Chem. 1989 Jan 5;264(1):380-8
references.pmid: 2491849
references.citation: J Invest Dermatol. 2002 Mar;118(3):409-15
references.pmid: 11874478
references.citation: Hautarzt. 2003 Oct;54(10):1003-15; quiz 1016-7
references.pmid: 14577419
references.citation: Arthritis Rheum. 2009 Sep;60(9):2817-21
references.pmid: 19714619
references.citation: J Biol Chem. 1999 Jan 22;274(4):2446-55
references.pmid: 9891015
references.citation: Acta Derm Venereol. 2005;85(3):216-20
references.pmid: 16040405
references.citation: Int J Surg. 2007 Aug;5(4):278-85
references.pmid: 17660136
references.citation: Int J Mol Med. 2008 Jul;22(1):55-60
references.pmid: 18575776
references.citation: Cell. 1992 Apr 3;69(1):11-25
references.pmid: 1555235
references.citation: J Dtsch Dermatol Ges. 2004 Apr;2(4):308-12
references.pmid: 16285329
references.citation: Philos Trans R Soc Lond B Biol Sci. 2004 May 29;359(1445):839-50
references.pmid: 15293811
references.citation: Ann Plast Surg. 1994 Aug;33(2):148-51
references.pmid: 7979045
references.citation: Int J Mol Med. 2009 Sep;24(3):283-93
references.pmid: 19639219