Novel functions of Stomatal Cytokinesis-Defective 1 (SCD1) in innate immune responses against bacteria.
Eukaryotes employ complex immune mechanisms for protection against microbial pathogens. Here, we identified SCD1 (Stomatal Cytokinesis-Defective 1), previously implicated in growth and development through its role in cytokinesis and polarized cell expansion (Falbel, T. G., Koch, L. M., Nadeau, J. A., Segui-Simarro, J. M., Sack, F. D., and Bednarek, S. Y. (2003) Development 130, 4011-4024) as a novel component of innate immunity. In Arabidopsis, SCD1 is a unique gene encoding for the only protein containing a complete DENN (Differentially Expressed in Normal and Neoplastic cells) domain. The DENN domain is a largely uncharacterized tripartite protein motif conserved among eukaryotic proteins. We show that conditional scd1-1 plants containing a point mutation in a conserved DENN residue affected a subset of signaling responses to some bacterial pathogen-associated molecular patterns (PAMPs). Consistent with increased transcript accumulation of Pathogen-related (PR) genes, scd1-1 plants were more resistant to Pseudomonas syringae pathovar tomato (Pst) DC3000 infection implicating SCD1 as a negative regulator of basal resistance against bacteria. scd1-1 plants were different from known mutants exhibiting constitutive expressor of PR (cpr)-like phenotypes, in that growth impairment of scd1-1 plants was genetically independent of constitutive immune response activation. For scd1-1, shift to elevated temperature or introduction of a mutant allele in Salicylic acid Induction-Deficient 2 (SID2) suppressed constitutive defense response activation. sid2-2 also repressed the resistance phenotype of scd1-1. Temperature shift and sid2-2, however, did not rescue conditional growth and sterility defects of scd1-1. These results implicate SCD1 in multiple cellular pathways, possibly by affecting different proteins. Overall, our studies identified a novel role for eukaryotic DENN proteins in immunity against bacteria.
Korasick
David A
DA
Interdisciplinary Plant Group (IPG), Division of Biochemistry, University of Missouri-Columbia, Columbia, Missouri 65211, USA.
McMichael
Colleen
C
Walker
Katie A
KA
Anderson
Jeffrey C
JC
Bednarek
Sebastian Y
SY
Heese
Antje
A
D016960:Agrobacterium tumefaciens / Q000502:physiology; D000595:Amino Acid Sequence; D018407:Bacterial Physiological Phenomena; D017353:Gene Deletion; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D010641:Phenotype; D063245:Plant Development; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D054046:Plant Stomata / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D010944:Plants / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013696:Temperature
D010940:Plant Proteins; D017382:Reactive Oxygen Species
Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):517-22
11756663
Curr Opin Plant Biol. 2007 Oct;10(5):466-72
17904410
Plant Cell. 2001 Oct;13(10):2225-40
11595798
Development. 2003 Sep;130(17):4011-24
12874123
Plant J. 2007 Sep;51(5):931-40
17651370
Plant Physiol. 2009 Aug;150(4):1638-47
19561123
Nat Cell Biol. 2008 Nov;10(11):1269-79
18849981
Cell. 2006 May 19;125(4):749-60
16713565
Plant Cell. 2004 Apr;16(4):1060-71
15031411
Nature. 2004 Apr 15;428(6984):764-7
15085136
J Biol Chem. 2010 Apr 2;285(14):10627-37
20154091
Mol Plant. 2008 May;1(3):423-45
19825551
Annu Rev Plant Biol. 2009;60:379-406
19400727
J Biol Chem. 2004 Dec 24;279(52):54264-74
15498773
Plant Cell. 2009 Sep;21(9):2884-97
19789277
Trends Plant Sci. 2003 Jun;8(6):263-71
12818660
Plant Physiol. 2007 Feb;143(2):661-9
17142480
Plant J. 2008 Mar;53(5):763-75
18005228
Mol Cell. 2010 Feb 12;37(3):370-82
20159556
Trends Mol Med. 2004 Oct;10(10):476-80
15464446
Cell. 2000 Dec 22;103(7):1111-20
11163186
J Biol Chem. 2006 Dec 15;281(50):38697-704
17043356
Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22522-7
20007779
Cell. 2006 Sep 8;126(5):969-80
16959575
Curr Biol. 2007 Jul 3;17(13):1109-15
17600708
Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12217-22
17626179
Biochem Biophys Res Commun. 2001 Sep 28;287(3):688-95
11563850
Mol Plant Microbe Interact. 2007 Aug;20(8):900-11
17722694
Trends Plant Sci. 2009 Apr;14(4):214-8
19303350
Cell. 1996 Sep 20;86(6):973-83
8808632
Nature. 2001 Nov 29;414(6863):562-5
11734859
Plant J. 1999 May;18(3):277-84
10377993
Plant Cell. 2008 Jan;20(1):152-67
18223036
Mol Biol Cell. 2001 May;12(5):1421-30
11359932
Nat Rev Immunol. 2005 Jun;5(6):446-58
15928677
Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4210-5
15007167
J Biol Chem. 2007 Apr 20;282(16):11715-21
17314102
FEBS Lett. 2007 Jul 10;581(17):3171-7
17572407
Annu Rev Phytopathol. 2006;44:135-62
16602946
Nature. 2007 Jul 26;448(7152):497-500
17625569
Plant J. 2008 Jan;53(2):393-9
17971037
Cell Host Microbe. 2007 May 17;1(3):175-85
18005697
EMBO J. 2008 Apr 23;27(8):1183-96
18354496
J Biol Chem. 2006 Dec 1;281(48):36969-76
17023433