title: Formation of oral and pharyngeal dentition in teleosts depends on differential recruitment of retinoic acid signaling.
abstract: One of the goals of evolutionary developmental biology is to link specific adaptations to changes in developmental pathways. The dentition of cypriniform fishes, which in contrast to many other teleost fish species possess pharyngeal teeth but lack oral teeth, provides a suitable model to study the development of feeding adaptations. Here, we have examined the involvement of retinoic acid (RA) in tooth development and show that RA is specifically required to induce the pharyngeal tooth developmental program in zebrafish. Perturbation of RA signaling at this stage abolished tooth induction without affecting the development of tooth-associated ceratobranchial bones. We show that this inductive event is dependent on RA synthesis from aldh1a2 in the ventral posterior pharynx. Fibroblast growth factor (FGF) signaling has been shown to be critical for tooth induction in zebrafish, and its loss has been associated with oral tooth loss in cypriniform fishes. Pharmacological treatments targeting the RA and FGF pathways revealed that both pathways act independently during tooth induction. In contrast, we find that in Mexican tetra and medaka, species that also possess oral teeth, both oral and pharyngeal teeth are induced independently of RA. Our analyses suggest an evolutionary scenario in which the gene network controlling tooth development obtained RA dependency in the lineage leading to the cypriniforms. The loss of pharyngeal teeth in this group was cancelled out through a shift in aldh1a2 expression, while oral teeth might have been lost ultimately due to deficient RA signaling in the oral cavity.
authors.affiliation: Molecular Zoology Group, Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, CNRS, INRA, UCB Lyon 1, Ecole Normale Supérieure de Lyon, 69364 Lyon Cedex 07, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Gibert
authors.forename: Laure
authors.initials: L
authors.lastname: Bernard
authors.forename: Melanie
authors.initials: M
authors.lastname: Debiais-Thibaud
authors.forename: Franck
authors.initials: F
authors.lastname: Bourrat
authors.forename: Jean-Stephane
authors.initials: JS
authors.lastname: Joly
authors.forename: Karen
authors.initials: K
authors.lastname: Pottin
authors.forename: Axel
authors.initials: A
authors.lastname: Meyer
authors.forename: Sylvie
authors.initials: S
authors.lastname: Retaux
authors.forename: David W
authors.initials: DW
authors.lastname: Stock
authors.forename: William R
authors.initials: WR
authors.lastname: Jackman
authors.forename: Pawat
authors.initials: P
authors.lastname: Seritrakul
authors.forename: Gerrit
authors.initials: G
authors.lastname: Begemann
authors.forename: Vincent
authors.initials: V
authors.lastname: Laudet
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D003817:Dentition; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000502:physiology; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005399:Fishes / Q000196:embryology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009990:Oryzias / Q000196:embryology* / Q000378:metabolism*; D010614:Pharynx / Q000196:embryology / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000819:agonists / Q000037:antagonists & inhibitors; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014212:Tretinoin / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D018168:Receptors, Retinoic Acid; D014212:Tretinoin; D005346:Fibroblast Growth Factors; D000444:Aldehyde Dehydrogenase
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