title: HLA-A, -B, and -DRB1 gene polymorphism in the Miao ethnic group of Hunan, China.
abstract: OBJECTIVE
  To investigate HLA-A, -B, and -DRB1 gene polymorphism in the Miao ethnic group in Hunan, China.
  
  
  METHODS
  PCR-sequence specific oligonucleotide probes (SSO) reverse flow chip method was used to type HLA-A, -B, and -DRB1 genes of 154 unrelated healthy Miao ethnic individuals in Hunan. The allele and haplotype frequencies were calculated and compared with other populations in China.
  
  
  RESULTS
  A total of 10 HLA-A, 25 HLA-B, and 13 HLA-DRB1 alleles were observed in the population. The higher frequency alleles included A 11(38.96%), A 02(27.27%), A 24(18.83%), B 40(60)(17.53%), B 46(13.96%), B 15(75)(11.69%), DRB1 09(15.26%), DRB1 12(15.26%), DRB1 15(15.26%), and DRB1 04(12.66%). The most frequent haplotypes were A11-B60(9.60%), A2-B46(9.27%), A11-B75(9.22%), B75-DR12(6.31%), A11-DR12(9.62%), A11-DR4(9.07%), A11-DR15(6.69%), A11-B75-DR12(5.43%), A11-B60-DR4(4.24%), and A2-B46-DR9(3.71%). Compared with other populations in China, HLA gene polymorphism of Hunan Miao population was close to that of southern population.
  
  
  CONCLUSIONS
  HLA loci are highly polymorphic in the Miao population of Hunan, and their distribution has the character of South China population.
authors.affiliation: Graduate School of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Qian
authors.forename: Yubin
authors.initials: Y
authors.lastname: Xie
authors.forename: Guangxiu
authors.initials: G
authors.lastname: Lu
mesh_terms: D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D005787:Gene Frequency; D005838:Genotype; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains