[Effect of amygdalin on serum proteinic biomarker in pulmonary fibrosis of bleomycin-induced rat].
OBJECTIVE
To evaluate effect of amygdalin on expression of four biomarkers in the animal model of pulmonary fibrosis induced by bleomycin.


METHODS
Rats were given one dose (5 mg/kg) of bleomycin in bleomycin-treated groups, amygdalin-treated groups and saline in controls by intratracheal instillation exposed surgically. The amygdalin-treated groups rats were treated with intraperitoneal injection of amygdalin (15 mg x kg(-1) x day(-1)). The rats were sacrificed 7, 14 and 28 days after bleomycin administration. Polarized light microscopy and Image-Pro Plus detected I and III collagen expressed in Paraffin-embedded lung sections stained with Sirius red. Surface-enhanced laser desorption-ionization time-of-flight mass spectrometry (SELDI-TOF MS) with weak cationic proteinchip (CM10) detected differentially expressed proteins in the pooled serum samples of all groups.


RESULTS
Consistent fibrotic responses were found in all bleomycin and amygdalin-tread groups. On the 7th, 14th and 28th day after bleomycin or saline instillation, four differentially expressed proteins were detected in the pooled serum of all groups rats, consisting of 4 proteins with mass/charge ratio of 3530.7, 7043.5, 8332.6 and 9068.0, respectively. Compared with control groups, protein peaks intensity ratio with mass/charge ratio of 3530.7 on 7, 28 d and 7043.5, 8332.6 and 9068.0 on 7, 14 and 28 d was > 2 in bleomycin-treated groups. Compared with amygdalin-treated groups, protein peaks intensity with mass/charge ratio of 3530.7 at 7, 14, 28 d had no change almost, but protein peaks intensity ratio with mass/charge ratio of 7043.5 at 7 d, 8332.6 on 28 d and 9068.0 on 14 d was > 2 in bleomycin-tread groups. All the four protein peaks intensity had no change almost at other point.


CONCLUSIONS
Amygdalin may reduce the bleomycin-induced increase of differentially expressed protein peak intensities in rat serum.
Du
Hai-Ke
HK
General Hospital of PLA, Beijing 100853, China.
Song
Fu-Cheng
FC
Zhou
Xin
X
Li
He
H
Zhang
Jian-Peng
JP
D000678:Amygdalin / Q000494:pharmacology*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001761:Bleomycin / Q000009:adverse effects*; D001798:Blood Proteins / Q000378:metabolism*; D008297:Male; D011658:Pulmonary Fibrosis / Q000097:blood* / Q000139:chemically induced; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D001798:Blood Proteins; D001761:Bleomycin; D000678:Amygdalin