Atomic force microscopy probing in the measurement of cell mechanics.
Atomic force microscope (AFM) has been used incrementally over the last decade in cell biology. Beyond its usefulness in high resolution imaging, AFM also has unique capabilities for probing the viscoelastic properties of living cells in culture and, even more, mapping the spatial distribution of cell mechanical properties, providing thus an indirect indicator of the structure and function of the underlying cytoskeleton and cell organelles. AFM measurements have boosted our understanding of cell mechanics in normal and diseased states and provide future potential in the study of disease pathophysiology and in the establishment of novel diagnostic and treatment options.
Kirmizis
Dimitrios
D
Department of Physics, Laboratory for Thin Films-Nanosystems and Nanometrology, Aristotle University, Thessaloniki, Greece. dkirmizi@physics.auth.gr
Logothetidis
Stergios
S
D000818:Animals; D002468:Cell Physiological Phenomena; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D008846:Micromanipulation / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D015336:Molecular Probe Techniques
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