title: Effects of gonadotropin-inhibitory hormone on folliculogenesis and steroidogenesis of cyclic mice.
abstract: OBJECTIVE
  To evaluate the effects of gonadotropin-inhibitory hormone (GnIH) treatment on ovarian activity of mice.
  
  
  METHODS
  Animal study.
  
  
  METHODS
  Reproductive physiology laboratory of university department of zoology.
  
  
  METHODS
  Twelve-week-old female mice of inbred Parkes strain.
  
  
  METHODS
  Mice treated with different doses of GnIH (control, 100 ng, 500 ng, and 2 μg per day) for 8 days were studied. For in vitro study, the ovaries of proestrus mice were cultured with different doses of GnIH for 24 hours at 37 °C.
  
  
  METHODS
  Folliculogenesis, steroidogenesis, luteogenesis, and apoptosis in the ovaries of control and GnIH-treated mice.
  
  
  RESULTS
  GnIH treatment produced significant changes in body mass, circulating steroid levels, and ovarian activity in the mice. GnIH also caused dose-dependent histologic changes in follicular development and luteinization. The antral follicles showed abnormal changes. The mice treated with increasing dose of GnIH showed significant changes in steroid synthesis owing to inhibitory effects of GnIH on ovarian expression of LH receptor, steroidogenic acute regulatory, and 3β-hydroxysteroid dehydrogenase proteins.
  
  
  CONCLUSIONS
  GnIH inhibited follicular development and steroidogenesis in the ovary of mice. This study thus suggests biologic significance of this neuropeptide in regulating ovarian activity.
authors.affiliation: Department of Zoology, Banaras Hindu University, Varanasi, India.
authors.forename: Padmasana
authors.initials: P
authors.lastname: Singh
authors.forename: Amitabh
authors.initials: A
authors.lastname: Krishna
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Tsutsui
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D005260:Female; D006023:Glycoproteins / Q000502:physiology*; D006062:Gonadotropins / Q000037:antagonists & inhibitors; D007028:Hypothalamic Hormones / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism*; D013256:Steroids / Q000096:biosynthesis*
chemical_list: D006023:Glycoproteins; D006062:Gonadotropins; D007028:Hypothalamic Hormones; D013256:Steroids; C014995:gonadotropin inhibitor