title: Evaluation of cytotoxic activity of Schisandra chinensis lignans.
abstract: Using exhaustive chromatographic separation we have isolated (-)-tigloyl-deangeloyl-gomisin F as a novel dibenzocyclooctadiene lignan from schisandra chinensis. With the help of HPLC, we further isolated (+)-schisandrin, (+)-deoxyschisandrin, (+)-γ-schisandrin, (-)-gomisin J, (+)-gomisin A, (-)-gomisin N, (-)-tigloyl-gomisin P, (-)-wuweizisu C, (-)-gomisin D, rubrisandrin A, (-)-gomisin G, (+)-gomisin K (3) and (-)-schisantherin C. A full NMR description of (-)-schisantherin C was carried out with the aim to confirm previous reports of its structure. Compounds isolated were identified on the basis of UV, IR, (1)H- and (13)C-NMR and MS. The cytotoxicity of lignans was tested for the BY-2 cell line alone and as a synergistic effect with the cytotoxic agent camptothecin. Lignans showed various toxicity and synergistic and antagonistic effects on camptothecin-induced cytotoxicity. Cytotoxicity against colon cancer cell line LoVo was also tested.
authors.affiliation: Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. karel.mejkal@post.cz
authors.forename: Karel
authors.initials: K
authors.lastname: Smejkal
authors.forename: Tereza
authors.initials: T
authors.lastname: Slapetová
authors.forename: Pavel
authors.initials: P
authors.lastname: Krmenčík
authors.forename: Petr
authors.initials: P
authors.lastname: Babula
authors.forename: Stefano
authors.initials: S
authors.lastname: Dall'Acqua
authors.forename: Gabbriella
authors.initials: G
authors.lastname: Innocenti
authors.forename: Ján
authors.initials: J
authors.lastname: Vančo
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Casarin
authors.forename: Maria
authors.initials: M
authors.lastname: Carrara
authors.forename: Karolína
authors.initials: K
authors.lastname: Kalvarová
authors.forename: Margita
authors.initials: M
authors.lastname: Dvorská
authors.forename: Jiří
authors.initials: J
authors.lastname: Slanina
authors.forename: Eva
authors.initials: E
authors.lastname: Kramářová
authors.forename: Ondřej
authors.initials: O
authors.lastname: Julínek
authors.forename: Marie
authors.initials: M
authors.lastname: Urbanová
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry; D032141:Schisandra / Q000737:chemistry*; D014026:Nicotiana / Q000187:drug effects
chemical_list: D003603:Cytotoxins; D017705:Lignans; D010936:Plant Extracts