title: Nematicidal natural products from the aerial parts of Buddleja crispa.
abstract: Studies on the aerial parts of Buddleja crispa yielded 13 known compounds, nonyl benzoate, hexyl p-hydroxy-cinnamate, ginipin, gardiol, 1-heptacosanol, steroidal galactoside (22 R)-stigmasta-7,9 (11)-dien-22 beta-ol-3beta-O-beta-D-galactopyranoside, 3-methoxy benzoic acid, beta-sitosterol and ursolic acid. Besides this two iridoid galactosides buddlejosides A, buddlejosides B and a benzofuran-type sesquiterpene buddlejone have been isolated from the ETOAC fraction of B. crispa. Together with the above compounds, methyl benzoate (1) and 3-methoxy-4-hydroxy benzoic acid (2) were also isolated. Compound 2 (C(8)H(8)O(4)) was identified by comparison of its data with those reported earlier, which was originally isolated from Onosma hispidum, and this is the first report of its isolation from this species. For compounds 1 and 2, the total alcoholic soluble extract, methanol soluble, chloroform soluble, ethyl acetate soluble and petroleum ether soluble extract of the aerial parts of B. crispa were screened for nematicidal activity against nematodes of freshly hatched second-stage juveniles of Meloidogyne incognita (root-knot nematode), exhibiting 92%, 40%, 88%, 83%, 82% and 50% mortality, respectively, of eloids M. incognita at 0.5% concentration. Compound 1 was more potent than the nematicide Azadirachta indica at the same concentration. Negative results were obtained for the nematicidal activity of petroleum ether extract of B. crispa leaves.
authors.affiliation: Pharmaceutical Research Center, PCSIR Laboratories Complex, Karachi-7520, Pakistan. nighat2001us@yahoo.com
authors.forename: Nighat
authors.initials: N
authors.lastname: Sultana
authors.forename: Musarrat
authors.initials: M
authors.lastname: Akhter
authors.forename: Rashid Ali
authors.initials: RA
authors.lastname: Khan
authors.forename: Nighat
authors.initials: N
authors.lastname: Afza
authors.forename: Rasool Bakh
authors.initials: RB
authors.lastname: Tareen
authors.forename: Abdul
authors.initials: A
authors.lastname: Malik
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000737:chemistry* / Q000494:pharmacology*; D001688:Biological Products; D032861:Buddleja / Q000737:chemistry*; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D014431:Tylenchoidea / Q000187:drug effects*
chemical_list: D000871:Anthelmintics; D001688:Biological Products; D010936:Plant Extracts