New stepwise cooling system for short-term porcine islet preservation.
OBJECTIVE
Porcine islets are the most suitable for xeno-islet transplantation. However, it is necessary to establish an effective preservation method against its fragility. Recently, we developed a new cooling and preservation (Keep and Fresh [KFC]; FUJIYA Co, Tokushima, Japan) system, which can maintain viability of hepatocyte. In this study, we examined the KFC for porcine islet preservation.


METHODS
Isolated porcine islets were preserved in CMRL 1066 culture media with bovine serum at 37°C, 22°C, and 4°C and KFC for 24, 48, and 72 hours. Islet recovery rate, purity, and viability were evaluated.


RESULTS
After 24-hour preservation, the recovery rate was the highest in the KFC, but no significant difference was found. After 48-hour preservation, the recovery rate by the KFC was 73.9% ± 17.3%, which was significantly higher than the other groups (48.7% ± 28.6% at 37°C, P < 0.01; 46.6% ± 15.5% at 22°C, P < 0.01; 61.5% ± 20.0% at 4°C, P < 0.05). After 72-hour preservation, the difference of recovery rate was clearer. In the KFC group, purities and viabilities were the highest among the groups after 24-, 48-, and 72-hour preservation.


CONCLUSIONS
The KFC system significantly improved porcine islet preservation; therefore, the KFC might be useful for porcine islet preservation.
Ikemoto
Tetsuya
T
Baylor Institute for Immunology Research, Baylor Research Institute, Dallas, TX, USA.
Noguchi
Hirofumi
H
Fujita
Yasutaka
Y
Takita
Morihito
M
Shimoda
Masayuki
M
Sugimoto
Koji
K
Jackson
Andrew
A
Naziruddin
Bashoo
B
Shimada
Mitsuo
M
Levy
Marlon F
MF
Matsumoto
Shinichi
S
D000818:Animals; D002470:Cell Survival; D007515:Islets of Langerhans / Q000502:physiology*; D016381:Islets of Langerhans Transplantation; D009926:Organ Preservation / Q000379:methods*; D013552:Swine