Effects of garlic oil on the migration of neutrophil-like cell studied by using a chemotactic gradient Labchip.
We have designed and fabricated a novel chemotactic gradient Labchip for studying cell migration quantitatively. Owing to the great potential of garlic and its preparations in developing antiinflammatory drugs, the aim of the present study is to investigate the effect of garlic oil on the locomotion of a neutrophil-like cell by measuring the dynamic features of cell migration including migration direction, average migration speed, chemotactic index (CI), and motility index (MI) with the newly designed Labchip. We found that garlic oil treatment lowered the values of CI and MI and reduced the average speed of cell migration from 13 to 8 microm/min. The results indicate that garlic oil is a potential inhibitor for neutrophil-like cell migration and chemotactic responsiveness. By comparing with the effects of nocodazole and cytochalasin B, we also suggest that the antiinflammatory activity exhibited by garlic oil was mainly through inhibiting the assembly-disassembly processes of the cytoskeleton.
Shih
Po-Chen
PC
Department of Electrophysics, National Chiao Tung University, Hsinchu 300, Taiwan.
Kuo
Chia-Hao
CH
Juang
Jenh-Yih
JY
Liu
Cheng-Hsien
CH
Hsu
Long
L
Liu
Cheng-Tzu
CT
D000498:Allyl Compounds / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology; D056656:Lab-On-A-Chip Devices; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects*; D012212:Rheology / Q000187:drug effects; D013440:Sulfides / Q000494:pharmacology*
D000498:Allyl Compounds; D016209:Interleukin-8; D013440:Sulfides; C038491:allyl sulfide
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