Single-molecule imaging of DNA duplexes immobilized on surfaces with a scanning tunneling microscope.
We succeeded for the first time in achieving single-molecule imaging of DNA duplexes vertically oriented on surfaces, with an in situ scanning tunneling microscope (STM), and demonstrated that this observation clearly revealed the surface hybridization behavior for both perfectly matched and mismatched DNA duplexes, at the single-molecule level.
Ohshiro
Takahito
T
Bioengineering Laboratory, RIKEN, Wako, Japan. tohshiro@riken.jp
Maeda
Mizuo
M
D020137:Base Pair Mismatch; D004277:DNA, Single-Stranded / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009693:Nucleic Acid Hybridization
D004277:DNA, Single-Stranded