[Kinetics of growth 2-D and 3-D cell models under influence of microenvironment].
A cocultivation system was developed to investigate the interactions between MCF-7 breast cancer epithelial cells and MT-4 (line human T-cell leukemia) by paracrine mechanism (no contact cocultivation). Viability and proliferation were determined in two fractions of MCF-7 cells from spheroid culture and monolayer. It has been shown that the influence of MT-4 reduced the number of cells MCF-7 in fraction of adhesive cells and did not influence on the suspension fraction. Proliferation was reduced in both fractions without MT-4. Cells viability was different in monolayer and spheroids with MT-4 and without MT-4; in monolayer it was lower, in spheroids it was higher. We think that, MT-4 by paracrine signal way stimulated proliferation MCF 7 cells, reduced adhesive to the bottom and led to MTS formation.
Garmanchuk
L V
LV
Perepelitsyna
E M
EM
Sidorenko
M V
MV
Ostapchenko
L I
LI
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D018920:Coculture Techniques; D003470:Culture Media; D006801:Humans; D007700:Kinetics; D019899:Paracrine Communication; D018874:Spheroids, Cellular
D003470:Culture Media