Analysis of Transcripts Expressed in One-Day-Old Larvae and Fifth Instar Silk Glands of Tasar Silkworm, Antheraea mylitta.
Antheraea mylitta is one of the wild nonmulberry silkworms, which produces tasar silk. An EST project has been undertaken to understand the gene expression profile of A. mylitta silk gland. Two cDNA libraries, one from the whole bodies of one-day-old larvae and the other from the silkglands of fifth instar larvae, were constructed and sequenced. A total of 2476 good-quality ESTs (1239 clones) were obtained and grouped into 648 clusters containing 390 contigs and 258 singletons to represent 467 potential unigenes. Forty-five sequences contained putative coding region, and represented potentially novel genes. Among the 648 clusters, 241 were categorized according to Gene Ontology hierarchy and showed presence of several silk and immune-related genes. The A. mylitta ESTs have been organized into a freely available online database "AmyBASE". These data provide an initial insight into the A. mylitta transcriptome and help to understand the molecular mechanism of silk protein production in a Lepidopteran species.
Maity
Samita
S
Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India.
Goel
Sagar I
SI
Roy
Sobhan
S
Ghorai
Suvankar
S
Bhattacharyya
Swati
S
Venugopalan
Aravind
A
Ghosh
Ananta K
AK
J Invertebr Pathol. 2003 May;83(1):1-9
12725805
Gene. 1983 Nov;25(2-3):263-9
6198242
BMC Genomics. 2008 Jul 17;9:338
18637161
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D101-3
14681368
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12
4504350
Anal Biochem. 1987 Apr;162(1):156-9
2440339
J Biol Chem. 2001 Nov 2;276(44):41377-82
11522783
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D138-41
14681378
BMC Bioinformatics. 2003 Sep 11;4:41
12969510
J Biol Chem. 1997 Dec 19;272(51):32360-9
9405444
DNA Res. 2004 Feb 29;11(1):27-35
15141943
Insect Biochem Mol Biol. 2003 May;33(5):541-59
12706633
BMC Bioinformatics. 2006 Jun 23;7:322
16796757
Insect Mol Biol. 2001 Oct;10(5):437-45
11881808
J Biol Chem. 1998 Jun 19;273(25):15423-8
9624126
Science. 2004 Dec 10;306(5703):1937-40
15591204
J Biol Chem. 2005 Apr 15;280(15):14923-31
15695807
BMC Bioinformatics. 2005 Jun 16;6:151
15960857
BMC Genomics. 2006 Jul 21;7:184
16857061
Comp Biochem Physiol B Biochem Mol Biol. 2007 May;147(1):129-34
17350301
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D115-9
14681372
Insect Biochem Mol Biol. 2007 Jul;37(7):655-66
17550822
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jul 1;62(Pt 7):669-71
16820690
Insect Biochem Mol Biol. 2003 Oct;33(10):1025-33
14505696
Bioinformatics. 1999 May;15(5):391-412
10366660
Proc Natl Acad Sci U S A. 1998 May 26;95(11):5857-64
9600884
Insect Biochem Mol Biol. 2001 Sep;31(10):1013-8
11483437