Structural characterization of a new statherin from pig parotid granules.
This study describes the identification and structural characterization of Sus scrofa statherin. HPLC-electrospray ionization mass spectrometry analysis on pig parotid secretory granule extracts evidenced a peptide with a molecular mass value of 5381.1 +/- 0.6 Da and its truncated form, devoid of the C-terminal Ala residue, with a molecular mass value of 5310.1 +/- 0.6 Da. The complete sequence of pig statherin gene was determined by sequencing the full-length cDNA obtained by rapid amplification of cDNA ends. The gene is 549 base pairs long and contains an open reading frame of 185 nucleotides, encoding a 42-amino acid secretory polypeptide with a signal peptide of 19 residues. This sequence presents some typical features of the four statherins characterized till now, showing the highest degree of amino acid identity with bovine (57%) and human statherin (39%). Pig statherin is mono-phoshorylated on Ser-3, while primate statherins already characterized are di-phosphorylated on Ser-2 and Ser-3. This difference, probably connected to the Asp-4 --> Glu substitution, suggests the involvement of the Golgi-casein kinase, which strictly recognizes the SX(E/pS) consensus sequence.
Manconi
Barbara
B
Dipartimento di Scienze Applicate ai Biosistemi, Università di Cagliari, Cittadella Universitaria, Monserrato, CA, Italy.
Fanali
Chiara
C
Cabras
Tiziana
T
Inzitari
Rosanna
R
Patamia
Maria
M
Scarano
Emanuele
E
Fiorita
Antonella
A
Vitali
Alberto
A
Castagnola
Massimo
M
Messana
Irene
I
Sanna
Maria Teresa
MT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003594:Cytoplasmic Granules / Q000737:chemistry*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010306:Parotid Gland / Q000737:chemistry* / Q000166:cytology; D012471:Salivary Proteins and Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D021241:Spectrometry, Mass, Electrospray Ionization; D034421:Sus scrofa
C018149:STATH protein, human; D012471:Salivary Proteins and Peptides