title: Structural characterization of a new statherin from pig parotid granules.
abstract: This study describes the identification and structural characterization of Sus scrofa statherin. HPLC-electrospray ionization mass spectrometry analysis on pig parotid secretory granule extracts evidenced a peptide with a molecular mass value of 5381.1 +/- 0.6 Da and its truncated form, devoid of the C-terminal Ala residue, with a molecular mass value of 5310.1 +/- 0.6 Da. The complete sequence of pig statherin gene was determined by sequencing the full-length cDNA obtained by rapid amplification of cDNA ends. The gene is 549 base pairs long and contains an open reading frame of 185 nucleotides, encoding a 42-amino acid secretory polypeptide with a signal peptide of 19 residues. This sequence presents some typical features of the four statherins characterized till now, showing the highest degree of amino acid identity with bovine (57%) and human statherin (39%). Pig statherin is mono-phoshorylated on Ser-3, while primate statherins already characterized are di-phosphorylated on Ser-2 and Ser-3. This difference, probably connected to the Asp-4 --> Glu substitution, suggests the involvement of the Golgi-casein kinase, which strictly recognizes the SX(E/pS) consensus sequence.
authors.affiliation: Dipartimento di Scienze Applicate ai Biosistemi, Università di Cagliari, Cittadella Universitaria, Monserrato, CA, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Manconi
authors.forename: Chiara
authors.initials: C
authors.lastname: Fanali
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cabras
authors.forename: Rosanna
authors.initials: R
authors.lastname: Inzitari
authors.forename: Maria
authors.initials: M
authors.lastname: Patamia
authors.forename: Emanuele
authors.initials: E
authors.lastname: Scarano
authors.forename: Antonella
authors.initials: A
authors.lastname: Fiorita
authors.forename: Alberto
authors.initials: A
authors.lastname: Vitali
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnola
authors.forename: Irene
authors.initials: I
authors.lastname: Messana
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Sanna
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003594:Cytoplasmic Granules / Q000737:chemistry*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010306:Parotid Gland / Q000737:chemistry* / Q000166:cytology; D012471:Salivary Proteins and Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D021241:Spectrometry, Mass, Electrospray Ionization; D034421:Sus scrofa
chemical_list: C018149:STATH protein, human; D012471:Salivary Proteins and Peptides