title: Human selenophosphate synthetase 1 has five splice variants with unique interactions, subcellular localizations and expression patterns.
abstract: Selenophosphate synthetase 1 (SPS1) is an essential cellular gene in higher eukaryotes. Five alternative splice variants of human SPS1 (major type, DeltaE2, DeltaE8, +E9, +E9a) were identified wherein +E9 and +E9a make the same protein. The major type was localized in both the nuclear and plasma membranes, and the others in the cytoplasm. All variants form homodimers, and in addition, the major type forms a heterodimer with DeltaE2, and DeltaE8 with +E9. The level of expression of each splice variant was different in various cell lines. The expression of each alternative splice variant was regulated during the cell cycle. The levels of the major type and DeltaE8 were gradually increased until G2/M phase and then gradually decreased. DeltaE2 expression peaked at mid-S phase and then gradually decreased. However, +E9/+E9a expression decreased gradually after cell cycle arrest. The possible involvement of SPS1 splice variants in cell cycle regulation is discussed.
authors.affiliation: Laboratory of Molecular Genetics and Genomics, School of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Kwang Hee
authors.initials: KH
authors.lastname: Lee
authors.forename: Myoung Sup
authors.initials: MS
authors.lastname: Shim
authors.forename: Hyein
authors.initials: H
authors.lastname: Shin
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Xu
authors.forename: Bradley A
authors.initials: BA
authors.lastname: Carlson
authors.forename: Dolph L
authors.initials: DL
authors.lastname: Hatfield
authors.forename: Byeong Jae
authors.initials: BJ
authors.lastname: Lee
mesh_terms: D017398:Alternative Splicing; D002453:Cell Cycle; D002460:Cell Line; D006367:HeLa Cells; D006801:Humans; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D010770:Phosphotransferases; C062762:selenophosphate synthetase
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