title: Isoform-dependent effects of apoE on doublecortin-positive cells and microtubule-associated protein 2 immunoreactivity following (137)Cs irradiation.
abstract: Previously we found apoE isoform-dependent effects of (137)Cs irradiation on cognitive function of female mice 3 months following irradiation. Alterations in the number of immature neurons and in the levels of the dendritic marker microtubule-associated protein 2 (MAP-2) might contribute to the cognitive changes following irradiation. Therefore, in the present study we determined if, following (137)Cs irradiation, there are apoE isoform-dependent effects on loss of doublecortin-positive neuroprogenitor cells or MAP-2 immumonoreactivity. In the dentate gyrus, CA1 and CA3 regions of the hippocampus, enthorhinal and sensorimotor cortex, and central and basolateral nuclei of the amygdala of apoE3 female mice, MAP-2 immunoreactivity increased 3 months following (137)Cs irradiation. In addition, at 8 h following irradiation, the number of doublecortin-positive cells was higher in apoE3 than apoE2 or apoE4 mice. Together, these data indicate that brains of apoE3 mice respond differently to (137)Cs irradiation than those of apoE2 or apoE4 mice.
authors.affiliation: Department of Behavioral Neuroscience, Oregon Health and Science University, Portland, OR 97239, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Villasana
authors.forename: Timothy
authors.initials: T
authors.lastname: Pfankuch
authors.forename: Jacob
authors.initials: J
authors.lastname: Raber
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000378:metabolism*; D002588:Cesium Radioisotopes; D000089622:Doublecortin Domain Proteins; D005260:Female; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000276:immunology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D009479:Neuropeptides / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000528:radiation effects
chemical_list: D001057:Apolipoproteins E; D002588:Cesium Radioisotopes; D000089622:Doublecortin Domain Proteins; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D020033:Protein Isoforms
references.citation: Neuroscience. 2006;137(2):413-23
references.pmid: 16330151
references.citation: Radiat Res. 2008 Nov;170(5):572-8
references.pmid: 18959467
references.citation: Science. 2004 Aug 27;305(5688):1295-8
references.pmid: 15272123
references.citation: J Clin Invest. 1998 Jul 1;102(1):130-5
references.pmid: 9649566
references.citation: Radiat Res. 2005 Oct;164(4 Pt 2):556-60
references.pmid: 16187787
references.citation: Neuroscience. 2003;119(3):635-42
references.pmid: 12809684
references.citation: J Clin Invest. 1999 Jun;103(11):1579-86
references.pmid: 10359567
references.citation: Ann Neurol. 2004 Mar;55(3):381-9
references.pmid: 14991816
references.citation: J Pediatr Psychol. 2005 Jan-Feb;30(1):65-78
references.pmid: 15610986
references.citation: Int J Radiat Oncol Biol Phys. 1995 Feb 15;31(4):983-98
references.pmid: 7860415
references.citation: Nat Med. 2002 Sep;8(9):955-62
references.pmid: 12161748
references.citation: Neurotoxicol Teratol. 1989 Jul-Aug;11(4):385-93
references.pmid: 2796895
references.citation: Cancer Res. 2003 Jul 15;63(14):4021-7
references.pmid: 12874001
references.citation: Brain Res. 1987 Apr 21;409(2):316-28
references.pmid: 3580879
references.citation: JAMA. 1997 Oct 22-29;278(16):1349-56
references.pmid: 9343467
references.citation: J Biol Chem. 1997 Jul 18;272(29):17972-80
references.pmid: 9218423
references.citation: J Cell Biol. 2002 Aug 5;158(3):541-9
references.pmid: 12163474
references.citation: Radiat Res. 2008 Oct;170(4):422-8
references.pmid: 19024648
references.citation: Science. 1988 Apr 29;240(4852):622-30
references.pmid: 3283935
references.citation: Nature. 1982 Jun 24;297(5868):681-3
references.pmid: 7088155
references.citation: Science. 2004 Aug 27;305(5688):1258-64
references.pmid: 15333832
references.citation: Brain Res. 1996 Apr 15;716(1-2):29-38
references.pmid: 8738217
references.citation: Neuroscience. 2000;99(1):33-41
references.pmid: 10924950
references.citation: Exp Brain Res. 1981;43(2):207-12
references.pmid: 7250266
references.citation: Radiat Res. 2004 Jul;162(1):39-47
references.pmid: 15222778
references.citation: J Neurosci Methods. 1997 Jul 18;75(1):41-7
references.pmid: 9262142
references.citation: Radiat Res. 2006 Dec;166(6):883-91
references.pmid: 17149978