title: Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials.
abstract: This study investigated the proliferation and differentiation potential of pluripotent mesenchymal cells on three resin-based restoratives using a typical pluripotent mesenchymal precursor cell line, C2C12. C2C12 cells were cultured for 3-21 days on cured specimens of a Bis-GMA/TEGDMA-based composite resin (APX; Clearfil AP-X), a 4-META/MMA-based resin cement (SB; Superbond C&B) or a HEMA-containing resin modified glass-ionomer (LC; Fuji Ionomer Type II LC). To examine the influences on differentiation potential, alkaline phosphatase (ALP) activity of the cells cultured on each material was determined. On APX and SB, cells adhered and proliferated well, and no significant influences on ALP activity were observed. In contrast, poor cell proliferation and significant suppression of ALP activity were observed for cells cultured on LC, similar to those cultured on a zinc oxide EBA cement used as a control material. Bis-GMA/TEGDMA-based composite resin and 4-META/MMA-based resin exhibited better biocompatibility for C2C12 cells than HEMA-containing resin modified glass-ionomer, suggesting a potential advantage of the former two resins to show smaller influences on regeneration of periapical or periodontal tissue.
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan. imazato@dent.osaka-u.ac.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Imazato
authors.forename: Daisuke
authors.initials: D
authors.lastname: Horikawa
authors.forename: Kahoru
authors.initials: K
authors.lastname: Takeda
authors.forename: Wakako
authors.initials: W
authors.lastname: Kiba
authors.forename: Naomi
authors.initials: N
authors.lastname: Izutani
authors.forename: Ranna
authors.initials: R
authors.lastname: Yoshikawa
authors.forename: Mikako
authors.initials: M
authors.lastname: Hayashi
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ebisu
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Nakano
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D003188:Composite Resins; D003793:Dental Restoration, Permanent; D005899:Glass Ionomer Cements; D062425:Hydroxybenzoate Ethers; D062385:Hydroxybenzoates; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008809:Mice, Inbred C3H; D039904:Pluripotent Stem Cells / Q000166:cytology*; D012038:Regeneration; D019279:Resin Cements; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D054457:Tissue Scaffolds; D015035:Zinc Oxide-Eugenol Cement / Q000737:chemistry
chemical_list: D003188:Composite Resins; D005899:Glass Ionomer Cements; D062425:Hydroxybenzoate Ethers; D062385:Hydroxybenzoates; D019279:Resin Cements; D013778:Tetrazolium Salts; D013844:Thiazoles; D015035:Zinc Oxide-Eugenol Cement; C015199:ethoxybenzoic acid; D000469:Alkaline Phosphatase; C022616:thiazolyl blue