title: Temporal adaptive changes in contractility and fatigability of diaphragm muscles from streptozotocin-diabetic rats.
abstract: Diabetes is characterized by ventilatory depression due to decreased diaphragm (DPH) function. This study investigated the changes in contractile properties of rat DPH muscles over a time interval encompassing from 4 days to 14 weeks after the onset of streptozotocin-induced diabetes, with and without insulin treatment for 2 weeks. Maximum tetanic force in intact DPH muscle strips and recovery from fatiguing stimulation were measured. An early (4-day) depression in contractile function in diabetic DPH was followed by gradual improvement in muscle function and fatigue recovery (8 weeks). DPH contractile function deteriorated again at 14 weeks, a process that was completely reversed by insulin treatment. Maximal contractile force and calcium sensitivity assessed in Triton-skinned DPH fibers showed a similar bimodal pattern and the same beneficial effect of insulin treatment. While an extensive analysis of the isoforms of the contractile and regulatory proteins was not conducted, Western blot analysis of tropomyosin suggests that the changes in diabetic DPH response depended, at least in part, on a switch in fiber type.
authors.affiliation: Muscle Biology Research Group (MUBIG), Schools of Nursing & Medicine, University of Missouri-Kansas City, Kansas City, MO 64108, USA.
authors.forename: Marco
authors.initials: M
authors.lastname: Brotto
authors.forename: Leticia
authors.initials: L
authors.lastname: Brotto
authors.forename: J-P
authors.initials: JP
authors.lastname: Jin
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Nosek
authors.forename: Andrea
authors.initials: A
authors.lastname: Romani
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003964:Diaphragm / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D009119:Muscle Contraction; D018763:Muscle Fatigue; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D014335:Tropomyosin / Q000737:chemistry / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D014335:Tropomyosin; D013311:Streptozocin
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