Mitigating effects of antioxidant properties of Artemisia campestris leaf extract on hyperlipidemia, advanced glycation end products and oxidative stress in alloxan-induced diabetic rats.
Artemisia campestris is used as antivenom and anti-inflammatory Tunisian folk medicine. Recently, increased oxidative stress was shown to play an important role in the etiology and pathogenesis of diabetes mellitus and its complications. This study was designed to examine the effects of A. campestris leaf aqueous extract (Ac) on alloxan-induced diabetic rats by measuring glycemia, lipid profile, lipid peroxidation (MDA), protein carbonyl content (PCO), advanced oxidation protein products (AOPP), activities of both non-enzymatic and enzymatic antioxidants. Results of our study showed an increase in blood glucose levels, total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), a decrease in high-density lipoprotein cholesterol (HDL-c) level and disturbed antioxidant enzyme activities (CAT, SOD, GPx) in the pancreatic tissue of diabetic rats. Furthermore, MDA, PCO and AOPP were elevated in the pancreas of the diabetic rats. The administration of Ac to diabetic rats at a dose of 200mgkg(-1)bw resulted in a significant reduction in glycemia, TC, TG, LDL-c, pancreas LPO, PCO and AOPP levels, CAT and GPx activities associated with an elevation of GSH content and SOD activity in comparison with diabetic group. We conclude that A. campestris aqueous extract may be effective for correcting hyperglycemia and preventing diabetic complications.
Sefi
Mediha
M
Animal Physiology Laboratory, Sciences Faculty, BP 1171, 3000 Sfax, Tunisia.
Fetoui
Hamadi
H
Makni
Mohamed
M
Zeghal
Najiba
N
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry* / Q000633:toxicity; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D005419:Flavonoids / Q000032:analysis; D005951:Glucose Tolerance Test; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006949:Hyperlipidemias / Q000097:blood / Q000188:drug therapy*; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010179:Pancreas / Q000201:enzymology / Q000473:pathology; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013634:Tannins / Q000032:analysis; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D001786:Blood Glucose; D005419:Flavonoids; D017127:Glycation End Products, Advanced; D000960:Hypolipidemic Agents; D007328:Insulin; D008055:Lipids; D010636:Phenols; D010936:Plant Extracts; D013634:Tannins; D017392:Thiobarbituric Acid Reactive Substances