title: Expression of inflammatory and insulin signaling genes in adipose tissue in response to elective surgery.
abstract: BACKGROUND
  The mechanisms behind postoperative insulin resistance and impaired glucose utilization are not fully understood.
  
  
  OBJECTIVE
  In this study, we aimed to specifically evaluate the transcription profile of genes in the insulin and adipokine signaling pathways in sc and omental adipose tissue after surgical injury.
  
  
  METHODS
  Relative expression of 21 target genes was analyzed in both sc and omental adipose tissue sampled at the beginning and at the end of operation.
  
  
  METHODS
  The study was conducted at a university-affiliated hospital.
  
  
  METHODS
  Twelve nondiabetic patients [seven females; age, 65 (range, 46-72) yr; body mass index, 24.8 (16.5-29.8) kg/m(2)] undergoing major abdominal surgery were included.
  
  
  METHODS
  The changes in mRNA levels were analyzed.
  
  
  RESULTS
  After surgery, both sc and omental adipose tissue mRNA levels of genes involved in the IL6 and nicotinamide phosphoribosyltransferase pathways were increased, whereas mRNA levels of insulin receptor substrate 1 and adiponectin were reduced (P < 0.05). TNF pathway genes were differently regulated between sc and omental adipose tissue, and glucose transporter 4 mRNA levels were decreased only in omental adipose tissue.
  
  
  CONCLUSIONS
  The transcriptional output of pivotal inflammatory and insulin signaling pathway genes is altered after surgery, and this pattern differs between different fat depots. This could be of importance for the metabolic aberrations associated to postsurgical complications, such as insulin resistance and hyperglycemia.
authors.affiliation: Department of Molecular Medicine and Surgery, Karolinska Institutet, Centre for Molecular Medicine, L8:00 Karolinska University Hospital, 171 76 Stockholm, Sweden. anna.witasp@ki.se
authors.forename: Anna
authors.initials: A
authors.lastname: Witasp
authors.forename: Louise
authors.initials: L
authors.lastname: Nordfors
authors.forename: Martin
authors.initials: M
authors.lastname: Schalling
authors.forename: Jonas
authors.initials: J
authors.lastname: Nygren
authors.forename: Olle
authors.initials: O
authors.lastname: Ljungqvist
authors.forename: Anders
authors.initials: A
authors.lastname: Thorell
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000368:Aged; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000235:genetics* / Q000378:metabolism; D015212:Inflammatory Bowel Diseases / Q000235:genetics / Q000378:metabolism / Q000601:surgery*; D007328:Insulin / Q000235:genetics* / Q000378:metabolism; D007333:Insulin Resistance / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics*
chemical_list: D051275:Glucose Transporter Type 4; D007328:Insulin; D012333:RNA, Messenger