title: CCL20/CCR6 expression profile in pancreatic cancer.
abstract: BACKGROUND
  CCL20 and its receptor CCR6 have been shown to play a role in the onset, development and metastatic spread of various gastrointestinal malignancies. In this study, the expression profile and clinical significance of the CCL20/CCR6 system in distinct benign, pre-malignant and malignant pancreatic tissues was investigated.
  
  
  METHODS
  Using RealTime-PCR, enzyme-linked immunosorbent assay (ELISA), Western Blot and immunohistochemistry, we have analyzed the expression profile of CCL20/CCR6 in resection specimens from patients with chronic pancreatitis (CP) (n = 22), pancreatic cystadenoma (PA) (n = 11) and pancreatic carcinoma (PCA) (n = 25) as well as in the respective matched normal pancreatic tissues.
  
  
  RESULTS
  CCL20 mRNA and protein was weakly expressed in normal pancreatic tissues and CP and PA specimens but significantly up-regulated in PCA (8-fold) as compared to the matched normal tissue (P < 0.05). Moreover, CCL20 mRNA and protein expression was significantly associated with advanced T-category in patients with PCA (P < 0.05). CCR6 mRNA showed a significant up-regulation in all three disease entities as compared to normal tissues (P < 0.05, respectively).
  
  
  CONCLUSIONS
  CCL20 and CCR6 were significantly up-regulated in PCA as compared to the normal pancreatic tissue and CCL20 was significantly associated with advanced T-category in PCA patients. This suggests that CCL20 and CCR6 play a role in the development and progression of PCA and may constitute potential targets for novel treatment strategies.
authors.affiliation: Dept. of General -, Visceral-, Vascular - and Paediatric Surgery, University of the Saarland, Homburg/Saar, Germany. claudia.rubie@uks.eu
authors.forename: Claudia
authors.initials: C
authors.lastname: Rubie
authors.forename: Vilma Oliveira
authors.initials: VO
authors.lastname: Frick
authors.forename: Pirus
authors.initials: P
authors.lastname: Ghadjar
authors.forename: Mathias
authors.initials: M
authors.lastname: Wagner
authors.forename: Henner
authors.initials: H
authors.lastname: Grimm
authors.forename: Benjamin
authors.initials: B
authors.lastname: Vicinus
authors.forename: Christoph
authors.initials: C
authors.lastname: Justinger
authors.forename: Stefan
authors.initials: S
authors.lastname: Graeber
authors.forename: Martin K
authors.initials: MK
authors.lastname: Schilling
mesh_terms: D000328:Adult; D000368:Aged; D054418:Chemokine CCL20 / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054399:Receptors, CCR6 / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C108355:CCL20 protein, human; C106311:CCR6 protein, human; D054418:Chemokine CCL20; D012333:RNA, Messenger; D054399:Receptors, CCR6
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