Disease association mapping in Drosophila can be replicated in the wild.
Association and linkage mapping have become important tools in understanding the genetics of complex traits, including diseases in humans. As the success of association mapping is reduced by small effect sizes and limited power, linkage studies in laboratory-based model systems are still heavily used. But whether the results of these studies can be replicated in natural populations has been questioned. Here, we show that a polymorphism in the gene ref(2)P, which had previously been linked to sigma virus resistance in Drosophila melanogaster under laboratory conditions, also provides resistance against the virus in female flies in a wild population in the field. This genetic association is thus upheld in spite of a known genotype-by-genotype interaction and environmental variation.
Wilfert
Lena
L
Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK. lb445@cam.ac.uk
Jiggins
Francis M
FM
D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics* / Q000821:virology; D005260:Female; D008040:Genetic Linkage; D007304:Insect Viruses; D008297:Male; D014777:Virus Diseases / Q000235:genetics*
Genetics. 2004 Aug;167(4):2127-31
15342546
Genetics. 1989 Nov;123(3):525-33
2557263
Genetics. 1993 Apr;133(4):943-54
8462852
Science. 2008 Nov 7;322(5903):881-8
18988837
C R Hebd Seances Acad Sci. 1953 Mar 9;236(10):1085-6
13059944
Mol Biol Evol. 1996 Jan;13(1):191-9
8583891
Proc Biol Sci. 2007 Aug 22;274(1621):2027-34
17550883
BMC Evol Biol. 2008 Jun 30;8:187
18590517
Genetics. 2008 Apr;178(4):2191-9
18430944
Nat Rev Genet. 2009 Aug;10(8):565-77
19584810
Mol Ecol. 2008 Sep;17(17):3800-7
18665899
Nature. 2007 Jun 7;447(7145):661-78
17554300
Trends Parasitol. 2001 Apr;17(4):189-94
11282509
Theor Appl Genet. 1992 Nov;85(2-3):186-9
24197303
Genetics. 2007 Apr;175(4):1987-97
17277365
Arch Virol. 1993;129(1-4):131-43
8470948
Mol Ecol. 2007 Sep;16(18):3947-54
17725574
J Med Entomol. 2002 Jan;39(1):84-8
11931276
Science. 1997 Apr 18;276(5311):425-8
9103203