Transaminations with isopropyl amine: equilibrium displacement with yeast alcohol dehydrogenase coupled to in situ cofactor regeneration.
Enantiopure chiral amines synthesis using omega-transaminases is hindered by an unfavourable equilibrium, but when using isopropylamine as the amine donor the equilibrium can be completely displaced by using a specific dehydrogenase in situ for removal of formed acetone.
Cassimjee
Karim Engelmark
KE
Division of Biochemistry, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, SE-106 91 Stockholm, Sweden.
Branneby
Cecilia
C
Abedi
Vahak
V
Wells
Andrew
A
Berglund
Per
P
D000096:Acetone / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000586:Amination; D000588:Amines / Q000138:chemical synthesis* / Q000378:metabolism; D011437:Propylamines / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013237:Stereoisomerism
D000588:Amines; D011437:Propylamines; D000096:Acetone; D000426:Alcohol Dehydrogenase; C035263:2-propylamine