Extraction and characterization of polygalacturonase of Fomes sclerodermeus produced by solid-state fermentation.
Polygalacturonase (PG) production by Fomes sclerodermeus using solid-state fermentation (SSF) was carried out. Maximal PG activity (26 U/gdw) was obtained between days 11 and 13 at the end of exponential growth. PG activity in the crude extract was more stable at pH 5-6 and 30 degrees C and had optimum activity at pH 5 and 50 degrees C. Optimal conditions for PG extraction were: one time extraction with Na2SO4 as solvent with 10 min. of agitation. In a scale-up system, PG activity per gram of dry substrate decreased about 60% compared with the activity obtained in an Erlenmeyer flask; however, high total PG activity was obtained.
Salariato
D
D
Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias, Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Diorio
L A
LA
Mouso
N
N
Forchiassin
F
F
D002458:Cell Fractionation / Q000379:methods; D055453:Coriolaceae / Q000201:enzymology* / Q000254:growth & development; D005285:Fermentation; D005656:Fungal Proteins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D009172:Mycology / Q000379:methods; D011096:Polygalacturonase / Q000302:isolation & purification*; D012997:Solvents; D013696:Temperature
D005656:Fungal Proteins; D012997:Solvents; D011096:Polygalacturonase