Characterization of a diguanylate cyclase from Shewanella woodyi with cyclase and phosphodiesterase activities.
Cyclic-di-GMP signaling is used by many bacteria to control biofilm formation. As biofilm formation is an important survival strategy for many bacteria, the synthesis and degradation of cyclic-di-GMP is tightly regulated by enzymes containing domains with conserved GGDEF and EAL sequence motifs, respectively. In this report we characterize a protein with both cyclase and phosphodiesterase activities and demonstrate that it contributes to secretion of the extracellular polysaccharide matrix, an important step in early biofilm formation.
Liu
Niu
N
Department of Chemistry, Stony Brook University, Stony Brook, NY 11790-3400, USA.
Pak
Taemee
T
Boon
Elizabeth M
EM
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism*; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics / Q000378:metabolism*; D020592:Shewanella / Q000201:enzymology*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D010727:Phosphoric Diester Hydrolases; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase