title: The role of C. elegans Ena/VASP homolog UNC-34 in neuronal polarity and motility.
abstract: Ena/VASP proteins mediate the effects of guidance cues on the actin cytoskeleton. The single C. elegans homolog of the Ena/VASP family of proteins, UNC-34, is required for the migrations of cells and growth cones. Here we show that unc-34 mutant alleles also interact genetically with Wnt mutants to reveal a role for unc-34 in the establishment of neuronal polarity along the C. elegans anterior-posterior axis. Our mutant analysis shows that eliminating UNC-34 function results in neuronal migration and polarity phenotypes that are enhanced at higher temperatures, revealing a heat-sensitive process that is normally masked by the presence of UNC-34. Finally, we show that the UNC-34 protein is expressed broadly and accumulates in axons and at the apical junctions of epithelial cells. While most mutants lacked detectable UNC-34, three unc-34 mutants that contained missense mutations in the EVH1 domain produced full-length UNC-34 that failed to localize to apical junctions and axons, supporting the role for the EVH1 domain in localizing Ena/VASP family members.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
authors.forename: Tinya
authors.initials: T
authors.lastname: Fleming
authors.forename: Shih-Chieh
authors.initials: SC
authors.lastname: Chien
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Vanderzalm
authors.forename: Megan
authors.initials: M
authors.lastname: Dell
authors.forename: Megan K
authors.initials: MK
authors.lastname: Gavin
authors.forename: Wayne C
authors.initials: WC
authors.lastname: Forrester
authors.forename: Gian
authors.initials: G
authors.lastname: Garriga
mesh_terms: D000483:Alleles; D000818:Animals; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D002465:Cell Movement; D003001:Cloning, Molecular; D003599:Cytoskeleton / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006358:Hot Temperature; D008954:Models, Biological; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010641:Phenotype; D017434:Protein Structure, Tertiary; D034622:RNA Interference
chemical_list: D029742:Caenorhabditis elegans Proteins; D009419:Nerve Tissue Proteins; C511070:unc-34 protein, C elegans
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