title: Proteomic analysis on the alteration of protein expression in gills of ayu (Plecoglossus altivelis) associated with salinity change.
abstract: Gill is the primary osmoregulatory organ for euryhaline fish to acclimate salinity change. The effect of salinity on gill proteome in ayu, Plecoglossus altivelis, was investigated by two-dimensional gel electrophoresis (2-DE) and matrix assisted laser desorption ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS). Eight of eighteen altered proteins were successfully identified. They are involved in osmoregulation, cytoskeleton, energy metabolism, and stress response. Our results showed that vinculin, echinoderm microtubule-associated protein like protein 1, pyruvate kinase, betaine-homocysteine methyltransferase (BHMT), transaldolase, glyceraldehyde 3-phosphate dehydrogenase, and heat shock protein 70 (HSP70) were down-regulated, whereas cofilin was up-regulated when ayu transferred from fresh water (FW) to brackish water (BW). Partial cDNA sequences of BHMT, HSP70, Na(+)/K(+) ATPase (NKA) alpha-subunit and 18S rRNA genes were subsequently determined and used for 2-DE data verification by real-time PCR. Gill BHMT and HSP70 mRNAs decreased significantly in BW-transferred ayu, while NKA alpha-subunit mRNA had no significant change. It was suggested that cell volume-regulatory response, especially the protection by the BHMT/betaine system might play an important role in ayu acclimation to salinity change.
authors.affiliation: Faculty of Life Science and Biotechnology, Ningbo University, Ningbo city 315211, People's Republic of China.
authors.forename: Xin J
authors.initials: XJ
authors.lastname: Lu
authors.forename: Jiong
authors.initials: J
authors.lastname: Chen
authors.forename: Zuo A
authors.initials: ZA
authors.lastname: Huang
authors.forename: Yu H
authors.initials: YH
authors.lastname: Shi
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
mesh_terms: D000818:Animals; D050940:Betaine-Homocysteine S-Methyltransferase / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D029941:Fish Proteins / Q000378:metabolism*; D005880:Gills / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D036441:Osmeriformes / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism; D054712:Salinity; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D029941:Fish Proteins; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D050940:Betaine-Homocysteine S-Methyltransferase; D000254:Sodium-Potassium-Exchanging ATPase