Discrimination of N6-methyl adenine in a specific DNA sequence.
We have developed a novel chemistry-based method to detect adenine methylation. A DNA probe functionalized with a formyl group discriminates between adenine and N6-methyl adenine by the formation of a selective interstrand cross-link.
Dohno
Chikara
C
The Institute of Scientific and Industrial Research (ISIR), Osaka University, Ibaraki 567-0047, Japan.
Shibata
Tomonori
T
Nakatani
Kazuhiko
K
D000225:Adenine / Q000031:analogs & derivatives* / Q000032:analysis; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry
D000225:Adenine; D004247:DNA; D004269:DNA, Bacterial; C000730567:6-methyladenine