Adsorption of alexa-labeled Bt toxin on mica, glass, and hydrophobized glass: study by normal scanning confocal fluorescence.
We studied the kinetics of adsorption of alexa-labeled Bt toxin Cry1Aa, in monomer and oligomer states, on muscovite mica, acid-treated hydrophilic glass, and hydrophobized glass, in the configuration of laminar flow of solution in a slit. Normal confocal fluorescence through the liquid volume allows the visualization of the concentration in solution over the time of adsorption, in addition to the signal due to the adsorbed molecules at the interface. The solution signal is used as calibration for estimation of interfacial concentration. We found low adsorption of the monomer compared to oligomers on the three types of surface. The kinetic adsorption barrier for oligomers increases in the order hydrophobized glass, muscovite mica, acid-treated hydrophilic glass. This suggests enhanced immobilization in soil if toxin is under oligomer state.
Janot
Jean-Marc
JM
Institut Européen des Membranes, Université Montpellier 2, UMR 5635 (ENSCM, CNRS, UM2), CC047, 34095 Montpellier Cedex 5, France.
Boissière
Michel
M
Thami
Thierry
T
Tronel-Peyroz
Emmanuel
E
Helassa
Nordine
N
Noinville
Sylvie
S
Quiquampoix
Hervé
H
Staunton
Siobhán
S
Déjardin
Philippe
P
D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004731:Endotoxins / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes; D005898:Glass / Q000737:chemistry*; D006460:Hemolysin Proteins / Q000032:analysis* / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009930:Organic Chemicals; D012989:Soil Pollutants / Q000032:analysis* / Q000737:chemistry; D012996:Solutions; D013499:Surface Properties
C417664:Alexa594; D000538:Aluminum Silicates; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D005456:Fluorescent Dyes; D006460:Hemolysin Proteins; D009930:Organic Chemicals; D012989:Soil Pollutants; D012996:Solutions; C030956:insecticidal crystal protein, Bacillus Thuringiensis; C011934:mica