Distinct contributions of rod, cone, and melanopsin photoreceptors to encoding irradiance.
Photoreceptive, melanopsin-expressing retinal ganglion cells (mRGCs) encode ambient light (irradiance) for the circadian clock, the pupillomotor system, and other influential behavioral/physiological responses. mRGCs are activated both by their intrinsic phototransduction cascade and by the rods and cones. However, the individual contribution of each photoreceptor class to irradiance responses remains unclear. We address this deficit using mice expressing human red cone opsin, in which rod-, cone-, and melanopsin-dependent responses can be identified by their distinct spectral sensitivity. Our data reveal an unexpectedly important role for rods. These photoreceptors define circadian responses at very dim "scotopic" light levels but also at irradiances at which pattern vision relies heavily on cones. By contrast, cone input to irradiance responses dissipates following light adaptation to the extent that these receptors make a very limited contribution to circadian and pupillary light responses under these conditions. Our data provide new insight into retinal circuitry upstream of mRGCs and optimal stimuli for eliciting irradiance responses.
Lall
Gurprit S
GS
Faculty of Life Sciences, AV Hill Building, University of Manchester, Manchester M13 9PT, UK.
Revell
Victoria L
VL
Momiji
Hiroshi
H
Al Enezi
Jazi
J
Altimus
Cara M
CM
Güler
Ali D
AD
Aguilar
Carlos
C
Cameron
Morven A
MA
Allender
Susan
S
Hankins
Mark W
MW
Lucas
Robert J
RJ
D000704:Analysis of Variance; D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology; D006801:Humans; D008027:Light; D055537:Light Signal Transduction / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D012160:Retina / Q000502:physiology; D017949:Retinal Cone Photoreceptor Cells / Q000502:physiology*; D017948:Retinal Rod Photoreceptor Cells / Q000502:physiology*; D017299:Rod Opsins / Q000502:physiology*; D013997:Time Factors; D014785:Vision, Ocular / Q000502:physiology*; D000099210:Melanopsin
D017299:Rod Opsins; D000099210:Melanopsin
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