title: Feasibility of in vivo 15N MRS detection of hyperpolarized 15N labeled choline in rats.
abstract: The increase of total choline in tumors has become an important biomarker in cancer diagnosis. Choline and choline metabolites can be measured in vivo and in vitro using multinuclear MRS. Recent in vivo(13)C MRS studies using labeled substrates enhanced via dynamic nuclear polarization demonstrated the tremendous potential of hyperpolarization for real-time metabolic studies. The present study demonstrates the feasibility of detecting hyperpolarized (15)N labeled choline in vivo in a rat head at 9.4 T. We furthermore report the in vitro (172 +/- 16 s) and in vivo (126 +/- 15 s) longitudinal relaxation times. We conclude that with appropriate infusion protocols it is feasible to detect hyperpolarized (15)N labeled choline in live animals.
authors.affiliation: Laboratory for Functional and Metabolic Imaging, Ecole Polytechnique Fédérale de Lausanne, EPFL-SB-IPMC-LIFMET, Office F1 602, Station 6, CH-1015 Lausanne, Switzerland. cristina.cudalbu@epfl.ch
authors.forename: Cristina
authors.initials: C
authors.lastname: Cudalbu
authors.forename: Arnaud
authors.initials: A
authors.lastname: Comment
authors.forename: Fiodar
authors.initials: F
authors.lastname: Kurdzesau
authors.forename: Ruud B
authors.initials: RB
authors.lastname: van Heeswijk
authors.forename: Kai
authors.initials: K
authors.lastname: Uffmann
authors.forename: Sami
authors.initials: S
authors.lastname: Jannin
authors.forename: Vladimir
authors.initials: V
authors.lastname: Denisov
authors.forename: Deniz
authors.initials: D
authors.lastname: Kirik
authors.forename: Rolf
authors.initials: R
authors.lastname: Gruetter
mesh_terms: D000818:Animals; D002794:Choline / Q000737:chemistry*; D007553:Isotope Labeling; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D009587:Nitrogen Isotopes / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009587:Nitrogen Isotopes; D002794:Choline