Isolation and characterization of ethanol-producing Schizosaccharomyces pombe CHFY0201.
Ethanol-producing yeast strain, CHFY0201 was isolated from soil in South Korea using an enrichment technique in a yeast peptone dextrose medium supplemented with 5% (w/v) ethanol at 30 degrees C. The phenotypic and physiological characteristics, as well as molecular phylogenetic analysis based on the D1/D2 domains of the large subunit (26S) rDNA gene and the internally transcribed spacer (ITS) 1+2 regions suggested that the CHFY0201 was novel strain of Schizosaccharomyces pombe. During shaking flask cultivation, the highest ethanol productivity and theoretical yield of S. pombe CHFY0201 in YPD media containing 9.5% total sugars was 0.59 +/- 0.01 g/l/h and 88.4 +/- 0.91%, respectively. Simultaneous saccharification and fermentation for ethanol production was carried out using liquefied cassava (Manihot esculenta) powder in a 5 l lab-scale jar fermenter at 32 degrees C for 66 h with an agitation speed of 120 rpm. Under these conditions, S. pombe CHFY0201 yielded a final ethanol concentration of 72.1 +/- 0.27 g/l and a theoretical yield of 82.7 +/- 1.52% at a maximum ethanol productivity of 1.16 +/- 0.07 g/l/h. These results suggest that S. pombe CHFY0201 is a potential producer for industrial bioethanol production.
Choi
Gi-Wook
GW
Changhae Institute of Cassava and Ethanol Research, Changhae Ethanol Co., LTD, Jeonju 561-203, Korea.
Um
Hyun-Ju
HJ
Kim
Mina
M
Kim
Yule
Y
Kang
Hyun-Woo
HW
Chung
Bong-Woo
BW
Kim
Yang-Hoon
YH
D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D002365:Manihot / Q000378:metabolism*; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D012988:Soil Microbiology
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S; D000431:Ethanol