title: [Analysis on VP1 gene variation of Coxsackie virus B5 for aseptic meningitis isolated from Zhejiang Province in 2008].
abstract: OBJECTIVE
  Study meningoencephalitis virus isolated from the Coxsackie B5 virus(CVB5 virus) VP1 gene characteristics of Zhejiang Province in 2008 and compare with other countries CVB5 prototype isolates, and to explore the relationship of variation of the Virus VP1 areas and epidemic viral meningoencephalitis
  
  
  METHODS
  Hep-2 and RD cells in cerebrospinal fluid and stool specimens for virus isolation, positive isolates combination with intestinal type of serum. On the separation of virus extracted RNA, and then RT-PCR amplified VP, gene CVB5 virus fragments, and purification of sequencing products, using DNAMAN and Bioedit analytical processing software.
  
  
  RESULTS
  The VP1 gene of CVB5 isolated from Zhejiang province meningoencephalitis viral in 2008 was 735bp. There were no missing and insertion of nucleotide . Strains isolated from ZJ/12/02 with the nucleotide and amino acid sequence of the highest phylogenetic tree showed that they were not the same branch.
  
  
  CONCLUSIONS
  The mutation caused by viral meningoencephalitis virus CVB5 Zhejiang province in 2008 was smaller. Viral meningoencephalitis distributed in some areas in the province had no significant prevalence.
authors.affiliation: ZheJiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Ge
authors.forename: Ju-Ying
authors.initials: JY
authors.lastname: Yan
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Gong
mesh_terms: D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D002681:China; D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008582:Meningitis, Aseptic / Q000821:virology*; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins