title: Crystallization and preliminary X-ray analysis of the ligand-binding domain of cAMP receptor protein.
abstract: The cyclic AMP receptor protein (CRP) from Escherichia coli regulates the expression of a large number of genes. In this work, CRP has been overexpressed, purified and digested by subtilisin and chymotrypsin. The fragments S-CRP (digested by subtilisin) and CH-CRP (digested by chymotrypsin) have been purified and crystallized. Crystals of S-CRP diffracted to 2.0 A resolution and belonged to space group P2(1), with unit-cell parameters a = 59.7, b = 75.1, c = 128.3 A, beta = 91.5 degrees . Crystals of CH-CRP diffracted to 2.8 A resolution and belonged to space group P222, with unit-cell parameters a = 45.8, b = 60.9, c = 205.6 A.
authors.affiliation: Department of Chemistry and Institute of Biomedical Science, Fudan University, Shanghai 200433, People's Republic of China.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Tao
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Haiping
authors.initials: H
authors.lastname: Liu
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Bao
authors.forename: Weimin
authors.initials: W
authors.lastname: Gong
authors.forename: Shaoning
authors.initials: S
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D002373:Cyclic AMP Receptor Protein / Q000737:chemistry*; D008024:Ligands; D008969:Molecular Sequence Data; D054730:Protein Interaction Domains and Motifs
chemical_list: D002373:Cyclic AMP Receptor Protein; D008024:Ligands
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