title: Improved transformation of the halo-tolerant yeast Zygosaccharomyces rouxii by electroporation.
abstract: To improve the transformation efficiency of Zygosaccharomyces rouxii by electroporation, glycerol was added to the electroporation buffer and the cells were frozen at -80 degrees C. These alterations drastically increased transformation efficiency, and we found that competent cells can be preserved at -80 degrees C without decreasing their transformation efficiency for at least 30 d.
authors.affiliation: Manufacturing Division, Yamasa Corporation, Choushi, Chiba, Japan. j_watanabe@yamasa.com
authors.forename: Jun
authors.initials: J
authors.lastname: Watanabe
authors.forename: Kenji
authors.initials: K
authors.lastname: Uehara
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Mogi
authors.forename: Kazushi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Yamazaki
mesh_terms: D018274:Electroporation / Q000379:methods*; D005615:Freezing; D055049:Salt Tolerance; D014170:Transformation, Genetic; D020068:Zygosaccharomyces / Q000166:cytology* / Q000235:genetics* / Q000502:physiology