Isolation and characterization of Psacotheasin, a novel Knottin-type antimicrobial peptide, from Psacothea hilaris.
We report the isolation and characterization of a novel knottin-type antimicrobial peptide from the yellow-spotted long-horned beetle Psacothea hilaris. A cDNA encoding a 56-mer knottin-type propeptide was identified and its predicted molecular mass and pI was 5.92 kDa and 8.28, respectively. A 34-mer mature peptide was also selected and named herein as psacotheasin. The antimicrobial activity of chemically synthesized psacotheasin against human bacterial pathogens was subsequently investigated. The results showed that psacotheasin exerted potent activities against both Gram-positive and Gram-negative bacterial strains. The present study suggests that psacotheasin can be applied to develop novel therapeutic agents.
Hwang
Jae-Sam
JS
Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon 441-100, Korea.
Lee
Juneyoung
J
Hwang
Bomi
B
Nam
Sung-Hee
SH
Yun
Eun-Young
EY
Kim
Seong-Ryul
SR
Lee
Dong Gun
DG
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D001517:Coleoptera / Q000737:chemistry*; D047168:Cystine Knot Motifs; D020869:Gene Expression Profiling; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D023181:Antimicrobial Cationic Peptides; D012333:RNA, Messenger