WRR4, a broad-spectrum TIR-NB-LRR gene from Arabidopsis thaliana that confers white rust resistance in transgenic oilseed Brassica crops.
White blister rust caused by Albugo candida (Pers.) Kuntze is a common and often devastating disease of oilseed and vegetable brassica crops worldwide. Physiological races of the parasite have been described, including races 2, 7 and 9 from Brassica juncea, B. rapa and B. oleracea, respectively, and race 4 from Capsella bursa-pastoris (the type host). A gene named WRR4 has been characterized recently from polygenic resistance in the wild brassica relative Arabidopsis thaliana (accession Columbia) that confers broad-spectrum white rust resistance (WRR) to all four of the above Al. candida races. This gene encodes a TIR-NB-LRR (Toll-like/interleukin-1 receptor-nucleotide binding-leucine-rich repeat) protein which, as with other known functional members in this subclass of intracellular receptor-like proteins, requires the expression of the lipase-like defence regulator, enhanced disease susceptibility 1 (EDS1). Thus, we used RNA interference-mediated suppression of EDS1 in a white rust-resistant breeding line of B. napus (transformed with a construct designed from the A. thaliana EDS1 gene) to determine whether defence signalling via EDS1 is functionally intact in this oilseed brassica. The eds1-suppressed lines were fully susceptible following inoculation with either race 2 or 7 isolates of Al. candida. We then transformed white rust-susceptible cultivars of B. juncea (susceptible to race 2) and B. napus (susceptible to race 7) with the WRR4 gene from A. thaliana. The WRR4-transformed lines were resistant to the corresponding Al. candida race for each host species. The combined data indicate that WRR4 could potentially provide a novel source of white rust resistance in oilseed and vegetable brassica crops.
Borhan
Mohammad Hossein
MH
Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada, S7N 0X2. borhanh@agr.gc.ca
Holub
Eric B
EB
Kindrachuk
Colin
C
Omidi
Mansour
M
Bozorgmanesh-Frad
Ghazaleh
G
Rimmer
S Roger
SR
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics*; D001937:Brassica / Q000235:genetics* / Q000382:microbiology; D020090:Chromosome Segregation / Q000235:genetics; D018556:Crops, Agricultural / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics* / Q000276:immunology; D000090263:Leucine-Rich Repeat Proteins; D009868:Oomycetes / Q000472:pathogenicity; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D010938:Plant Oils / Q000378:metabolism; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics*; D034622:RNA Interference; D012639:Seeds / Q000235:genetics / Q000382:microbiology; D014774:Virulence
D029681:Arabidopsis Proteins; D000090263:Leucine-Rich Repeat Proteins; D010938:Plant Oils; D011506:Proteins; C532021:WRR4 protein, Arabidopsis
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