title: [Expression of B7-H1 on peripheral myeloid dendritic cells in patients with HIV infection and its correlation with diseases progression].
abstract: OBJECTIVE
  To investigate the features of B7-H1 expression on peripheral myeloid dendritic cells (mDCs) in patients with HIV infection and evaluate the correlations between B7-H1 expression and disease progression.
  
  
  METHODS
  Peripheral blood samples from 82 treatment-naïve patients with HIV infection, 28 viral complete responders (CRs) under antiretroviral therapy (ART) and 14 healthy controls (HCs) were collected. Flow cytometry was applied to investigate the expression of B7-H1 on mDCs and CD4 cell counts. Plasma HIV-1 viral load was detected by bDNA.
  
  
  RESULTS
  The frequency of B7-H1 expression on mDCs were 14.15% +/- 2.63%, 3.31% +/- 0.51% and 0.52% +/- 0.10% in AIDS patients, asymptomatic HIV infected individuals and HCs respectively. As compared with HCs, B7-H1 was significantly up-regulated on mDCs in HIV/AIDS patients. The order was as follows: AIDS patients > asymptomatic HIV infected individuals > HCs (all P < 0.05). Interestingly, the expression of B7-H1 on mDCs in long-term nonprogressors (LTNPs) was 3.12% +/- 1.14%. And it was lower than that in typical progressors (TPs) [8.12% +/- 1.37% (P = 0.001)]. Moreover, the expression of B7-H1 was negatively correlated with CD4 cell counts and positively correlated with plasma viral load in these patients (r = -0.631, P < 0.01 and r = 0.482, P < 0.01 respectively). The expression of B7-H1 on mDCs was significantly lower in ART complete responders than that in AIDS patients (6.59% +/- 1.43% vs 14.15% +/- 2.63%) (P < 0.01). Expression of B7-H1 on mDCs decreased markedly in patients whose CD4 cell counts greatly elevated after a successful antiretroviral treatment.
  
  
  CONCLUSIONS
  The expression of B7-H1 on mDCs is significantly up-regulated in HIV/AIDS patients. With a close correlation with disease status, it acts as a marker of disease progression.
authors.affiliation: Yunnan Provincial Hospital of Infectious Diseases, Yunnan AIDS Care Center, Yunnan 650301, China. wxch62597@21cn.com
authors.forename: Xi-cheng
authors.initials: XC
authors.lastname: Wang
authors.forename: Yi-shan
authors.initials: YS
authors.lastname: Fan
authors.forename: Zeng-quan
authors.initials: ZQ
authors.lastname: Zhou
authors.forename: Hui-qin
authors.initials: HQ
authors.lastname: Li
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Xin-ping
authors.initials: XP
authors.lastname: Yang
authors.forename: Lin
authors.initials: L
authors.lastname: Mao
authors.forename: Rong-hui
authors.initials: RH
authors.lastname: Xie
authors.forename: Su-yun
authors.initials: SY
authors.lastname: Lei
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Fu-sheng
authors.initials: FS
authors.lastname: Wang
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000473:pathology / Q000821:virology; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D060890:B7-H1 Antigen; D016022:Case-Control Studies; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D015658:HIV Infections / Q000097:blood* / Q000473:pathology / Q000821:virology; D006801:Humans; D008297:Male; D008875:Middle Aged; D019562:Viral Load; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D060890:B7-H1 Antigen; C423236:CD274 protein, human