title: [The levels of dioxin in 60 human breast milk samples and the exposure risk factor analysis in Shenzhen].
abstract: OBJECTIVE
  To investigate the levels of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzo-p-furans (PCDD-Fs) in human breast milk of the mothers who lived in non-directly persistent organic pollutants (POPs) polluted area in Shenzhen, and the correlation of exposure risk factor was analyzed.
  
  
  METHODS
  From July to November in 2007, 60 primiparas by vaginal delivery after parturition 3 weeks to 2 months were sampled for breast milk who aged from 20 - 34 years old and has lived in Shenzhen non-directly POPs polluted areas over 5 years. PCDD-Fs were extracted from the frozen-dried samples with accelerated solvent extraction (ASE), cleaned up by fluid management system (FMS) and quantified by high-resolution gas chromatography-high resolution mass spectrometry (HRGC-HRMS) using isotope dilution methodology. TEQ were calculated. The correlation relationship among infant's birth weight and length, participatory's dietary, age, inhabitation period, environment and the body burden of PCDD-Fs in mother was statistically analyzed by SPSS 13.0.
  
  
  RESULTS
  The participants aged from 20 - 34 years old (28 years on average) and lived in Shenzhen for 5 - 29 years (10 years on average). The concentration of PCDD-Fs in 60 breast milk samples were 26.957 143 - 669.583 333 pg/g fat (mean: 7.224 817 pg/g fat, median: 84.176 062 pg/g fat), and TEQ-PCDD-Fs in samples were 2.420 793 - 29.014 277 pg/g fat (mean: 8.645 992 pg/g fat, median: 7.751 804 pg/g fat). 2, 3, 4, 7, 8-PeCDF, 1, 2, 3, 7, 8-PeCDD, 2, 3, 7, 8-TCDD were the dominant contributors to the total TEQ, which were 3.691 654 pg/g fat (42.689 378%), 2.478 315 pg/g fat (28.652 356%), and 0.980 995 pg/g fat (11.343 995%) respectively. Significant positive correlations were found among age (r = 0.26, P < 0.05), inhabitation period (r = 0.49, P < 0.05), the consumption of fish (r = 0.37, P < 0.05) and the concentrations of TEQ-PCDD-Fs in the study.
  
  
  CONCLUSIONS
  The levels of dioxin chemicals in the breast milk samples in non-directly POPs polluted areas of Shenzhen are high. Significant positive correlations were found among age, inhabitation period, the consumption of fish and the concentration of PCDD-Fs.
authors.affiliation: POPs Lab, Shenzhen Center for Disease Prevention and Control, Shenzhen 518020, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Deng
authors.forename: Jian-qing
authors.initials: JQ
authors.lastname: Zhang
authors.forename: Li-shi
authors.initials: LS
authors.lastname: Zhang
authors.forename: You-sheng
authors.initials: YS
authors.lastname: Jiang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000328:Adult; D002681:China; D004147:Dioxins / Q000032:analysis*; D004781:Environmental Exposure; D005260:Female; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D009035:Mothers; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives / Q000032:analysis; D018570:Risk Assessment; D055815:Young Adult
chemical_list: D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; C038902:1,4-dioxin; C051452:1,2,3,7,8-pentachlorodibenzo-p-dioxin; C020887:dibenzo(1,4)dioxin