title: Adsorption of alexa-labeled Bt toxin on mica, glass, and hydrophobized glass: study by normal scanning confocal fluorescence.
abstract: We studied the kinetics of adsorption of alexa-labeled Bt toxin Cry1Aa, in monomer and oligomer states, on muscovite mica, acid-treated hydrophilic glass, and hydrophobized glass, in the configuration of laminar flow of solution in a slit. Normal confocal fluorescence through the liquid volume allows the visualization of the concentration in solution over the time of adsorption, in addition to the signal due to the adsorbed molecules at the interface. The solution signal is used as calibration for estimation of interfacial concentration. We found low adsorption of the monomer compared to oligomers on the three types of surface. The kinetic adsorption barrier for oligomers increases in the order hydrophobized glass, muscovite mica, acid-treated hydrophilic glass. This suggests enhanced immobilization in soil if toxin is under oligomer state.
authors.affiliation: Institut Européen des Membranes, Université Montpellier 2, UMR 5635 (ENSCM, CNRS, UM2), CC047, 34095 Montpellier Cedex 5, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Janot
authors.forename: Michel
authors.initials: M
authors.lastname: Boissière
authors.forename: Thierry
authors.initials: T
authors.lastname: Thami
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Tronel-Peyroz
authors.forename: Nordine
authors.initials: N
authors.lastname: Helassa
authors.forename: Sylvie
authors.initials: S
authors.lastname: Noinville
authors.forename: Hervé
authors.initials: H
authors.lastname: Quiquampoix
authors.forename: Siobhán
authors.initials: S
authors.lastname: Staunton
authors.forename: Philippe
authors.initials: P
authors.lastname: Déjardin
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004731:Endotoxins / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes; D005898:Glass / Q000737:chemistry*; D006460:Hemolysin Proteins / Q000032:analysis* / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009930:Organic Chemicals; D012989:Soil Pollutants / Q000032:analysis* / Q000737:chemistry; D012996:Solutions; D013499:Surface Properties
chemical_list: C417664:Alexa594; D000538:Aluminum Silicates; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D005456:Fluorescent Dyes; D006460:Hemolysin Proteins; D009930:Organic Chemicals; D012989:Soil Pollutants; D012996:Solutions; C030956:insecticidal crystal protein, Bacillus Thuringiensis; C011934:mica