Proteomics-based confirmation of protein expression and correction of annotation errors in the Brucella abortus genome.
BACKGROUND
Brucellosis is a major bacterial zoonosis affecting domestic livestock and wild mammals, as well as humans around the globe. While conducting proteomics studies to better understand Brucella abortus virulence, we consolidated the proteomic data collected and compared it to publically available genomic data.


RESULTS
The proteomic data was compiled from several independent comparative studies of Brucella abortus that used either outer membrane blebs, cytosols, or whole bacteria grown in media, as well as intracellular bacteria recovered at different times following macrophage infection. We identified a total of 621 bacterial proteins that were differentially expressed in a condition-specific manner. For 305 of these proteins we provide the first experimental evidence of their expression. Using a custom-built protein sequence database, we uncovered 7 annotation errors. We provide experimental evidence of expression of 5 genes that were originally annotated as non-expressed pseudogenes, as well as start site annotation errors for 2 other genes.


CONCLUSIONS
An essential element for ensuring correct functional studies is the correspondence between reported genome sequences and subsequent proteomics studies. In this study, we have used proteomics evidence to confirm expression of multiple proteins previously considered to be putative, as well as correct annotation errors in the genome of Brucella abortus strain 2308.
Lamontagne
Julie
J
Caprion Proteomics Inc, 7150 Alexander-Fleming, Montreal, Quebec, Canada.
Béland
Maxime
M
Forest
Anik
A
Côté-Martin
Alexandra
A
Nassif
Najib
N
Tomaki
Fadi
F
Moriyón
Ignacio
I
Moreno
Edgardo
E
Paramithiotis
Eustache
E
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D002003:Brucella abortus / Q000737:chemistry* / Q000235:genetics; D002460:Cell Line; D016680:Genome, Bacterial; D051379:Mice; D008969:Molecular Sequence Data; D009876:Operon; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D040901:Proteomics
D001426:Bacterial Proteins; D020543:Proteome
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