Cytotoxic effects of White-MTA and MTA-Bio cements on odontoblast-like cells (MDPC-23).
This study evaluated the cytotoxic effects of 2 mineral trioxide aggregate (MTA) cements - White-MTA-Angelus and a new formulation, MTA-Bio - on odontoblast-like cell (MDPC-23) cultures. Twenty-four disc-shaped (2 mm diameter x 2 mm thick) specimens were fabricated from each material and immersed individually in wells containing 1 mL of DMEM culture medium for either 24 h or 7 days to obtain extracts, giving rise to 4 groups of 12 specimens each: G1 - White-MTA/24 h; G2 - White-MTA/7 days; G3 - MTA-Bio/24 h; and G4 - MTA-Bio/7 days. Plain culture medium (DMEM) was used as a negative control (G5). Cells at 30,000 cells/cm(2) concentration were seeded in the wells of 24-well plates and incubated in a humidified incubator with 5% CO2 and 95% air at 37 degrees C for 72 h. After this period, the culture medium of each well was replaced by 1 mL of extract (or plain DMEM in the control group) and the cells were incubated for additional 2 h. Cell metabolism was evaluated by the MTT assay and the data were analyzed statistically by ANOVA and Tukey's test (alpha=0.05). Cell morphology and the surface of representative MTA specimens of each group were examined by scanning electron microscopy. There was no statistically significant difference (p>0.05) between G1 and G2 or between G3 and G4. No significant difference (p>0.05) was found between the experimental and control groups either. Similar cell organization and morphology were observed in all groups, regardless of the storage periods. However, the number of cells observed in the experimental groups decreased compared to the control group. MTA-Bio presented irregular surface with more porosities than White-MTA. In conclusion, White-MTA and MTA-Bio presented low cytotoxic effects on odontoblast-like cell (MDPC-23) cultures.
Lessa
Fernanda Campos Rosetti
FC
Department of Orthodontics and Pediatric Dentistry, Araraquara Dental School, São Paulo State University, Araraquara, SP, Brazil.
Aranha
Andreza Maria Fábio
AM
Hebling
Josimeri
J
Costa
Carlos Alberto de Souza
CA
D017607:Aluminum Compounds / Q000737:chemistry / Q000633:toxicity*; D017610:Calcium Compounds / Q000737:chemistry / Q000633:toxicity*; D002452:Cell Count; D018929:Cell Culture Techniques; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D003470:Culture Media; D003738:Dental Cements / Q000737:chemistry / Q000633:toxicity*; D004338:Drug Combinations; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009804:Odontoblasts / Q000187:drug effects*; D010087:Oxides / Q000737:chemistry / Q000633:toxicity*; D016062:Porosity; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000633:toxicity*; D017640:Silicates / Q000737:chemistry / Q000633:toxicity*; D013053:Spectrophotometry; D013385:Succinate Dehydrogenase / Q000032:analysis; D013499:Surface Properties; D013696:Temperature; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004396:Coloring Agents; D003470:Culture Media; D003738:Dental Cements; D004338:Drug Combinations; C525641:MTA Bio; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; C086631:mineral trioxide aggregate; D013385:Succinate Dehydrogenase; C022616:thiazolyl blue