title: Preliminary X-ray crystallographic analysis of SMU.2055 protein from the caries pathogen Streptococcus mutans.
abstract: The SMU.2055 gene from the major caries pathogen Streptococcus mutans is annotated as a putative acetyltransferase with 163 amino-acid residues. In order to identify its function via structural studies, the SMU.2055 gene was cloned into the expression vector pET28a. Native and SeMet-labelled SMU.2055 proteins with a His(6) tag at the N-terminus were expressed at a high level in Escherichia coli strain BL21 (DE3) and purified to homogeneity by Ni(2+)-chelating affinity chromatography. Diffraction-quality crystals of SeMet-labelled SMU.2055 were obtained using the sitting-drop vapour-diffusion method and diffracted to a resolution of 2.5 A on beamline BL17A at the Photon Factory, Tsukuba, Japan. The crystals belong to the orthorhombic space group C222(1), with unit-cell parameters a = 92.0, b = 95.0, c = 192.2 A. The asymmetric unit contained four molecules, with a solvent content of 57.1%.
authors.affiliation: School of Stomatology, Peking University, Beijing 100081, People's Republic of China.
authors.forename: Wang Hong
authors.initials: WH
authors.lastname: Zhao
authors.forename: Xiu Rong
authors.initials: XR
authors.lastname: Zhan
authors.forename: Xiong Zhuo
authors.initials: XZ
authors.lastname: Gao
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.forename: Yi Fei
authors.initials: YF
authors.lastname: Zhang
authors.forename: Jiuxiang
authors.initials: J
authors.lastname: Lin
authors.forename: Lan Fen
authors.initials: LF
authors.lastname: Li
authors.forename: Shi Cheng
authors.initials: SC
authors.lastname: Wei
authors.forename: Xio Dong
authors.initials: XD
authors.lastname: Su
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry*; D018360:Crystallography, X-Ray; D013295:Streptococcus mutans / Q000201:enzymology*
chemical_list: D000123:Acetyltransferases
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