Application of laser-assisted microdissection for gene expression analysis of mammalian germ cells.
Laser-assisted microdissection (LAM) is an important method to provide new significant insights into many embryological processes. To understand these processes, it is important to obtain specific populations of cells from complex tissue in an efficient and precise manner and to combine with many different molecular biological methods. During the last few years, the sophistication of the techniques of LAM has increased significantly and made the procedure easy to use. New micro-extraction protocols for DNA, RNA and proteins now allow broad downstream applications in the fields of genomics, transcriptomics and proteomics. In this review, we give a short overview of the application of LAM in combination with quantitative qPCR for the analysis of gene expression in mammalian germ cells.
Kenngott
R
R
Lehrstuhl für Tieranatomie II, Department of Veterinary Sciences, LMU München, D-80539 Munich, Germany.
Al-Banaw
A
A
Vermehren
M
M
Wendl
J
J
Sinowatz
F
F
D000818:Animals; D005260:Female; D015870:Gene Expression; D023281:Genomics; D005854:Germ Cells / Q000378:metabolism*; D007834:Lasers; D008297:Male; D008322:Mammals / Q000235:genetics / Q000378:metabolism; D042282:Microdissection / Q000379:methods*; D016133:Polymerase Chain Reaction; D040901:Proteomics