title: Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays.
abstract: We demonstrate the feasibility of a nanopore based single-molecule DNA sequencing method, which employs multicolor readout. Target DNA is converted according to a binary code, which is recognized by molecular beacons with two types of fluorophores. Solid-state nanopores are then used to sequentially strip off the beacons, leading to a series of detectable photon bursts, at high speed. We show that signals from multiple nanopores can be detected simultaneously, allowing straightforward parallelization to large nanopore arrays.
authors.affiliation: Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA.
authors.forename: Ben
authors.initials: B
authors.lastname: McNally
authors.forename: Alon
authors.initials: A
authors.lastname: Singer
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Yu
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Sun
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Weng
authors.forename: Amit
authors.initials: A
authors.lastname: Meller
mesh_terms: D004270:DNA, Circular / Q000737:chemistry*; D036103:Nanotechnology; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004270:DNA, Circular
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