IsiA is required for the formation of photosystem I supercomplexes and for efficient state transition in synechocystis PCC 6803.
Iron deficiency and other stress conditions strongly impact photosynthetic apparatus in photosynthetic organisms. Two novel chlorophyll (Chl)-containing supercomplexes (F4 and F5) in addition to the photosystem (PS) I trimers (F3) were observed by sucrose gradient ultracentrifugation in Synechocystis PCC 6803 under extensive iron starvation. 77K fluorescence and Western blot analyses of these supercomplexes revealed that they all contained IsiA. The F4 was identified as an IsiA-PSI-PSII supercomplex, while the F5 was assigned as an IsiA-PSI supercomplex. Deletion of isiA resulted in diminishing the PSI trimers (including the PSI trimers in iron-replete cells) and the two novel PSI supercomplexes (F4 and F5), and a significant reduction in the saturated whole-chain electron transport rate. However, the maximum PSII activities remained at levels similar to those of the wild type under various light conditions. The isiA(-) mutant was defective in state transition and sensitive to high light. The sensitivity of the isiA(-) mutant to high light was correlated with a higher level of membrane peroxidation. These results demonstrated that IsiA is required for the formation of PSI trimers and other higher complexes, and that IsiA is critical for efficient state transition.
Wang
Qiang
Q
Department of Applied Science, University of Arkansas, Little Rock, Arkansas, United States of America.
Hall
Camille L
CL
Al-Adami
Mustafa Z
MZ
He
Qingfang
Q
D001426:Bacterial Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002244:Carbon / Q000378:metabolism; D002338:Carotenoids / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002734:Chlorophyll / Q000378:metabolism; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D017353:Gene Deletion; D007501:Iron / Q000378:metabolism; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism*; D015227:Lipid Peroxidation; D009154:Mutation / Q000235:genetics; D018384:Oxidative Stress; D010788:Photosynthesis; D045331:Photosystem I Protein Complex / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000378:metabolism; D013312:Stress, Physiological; D046939:Synechocystis / Q000254:growth & development / Q000378:metabolism*; D020524:Thylakoids / Q000378:metabolism
D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D002734:Chlorophyll; C076726:chlorophyll A binding protein CP43, Cyanobacteria; D002338:Carotenoids; D002244:Carbon; D007501:Iron
Arch Microbiol. 2003 Dec;180(6):471-83
14605795
Plant Cell. 2007 Feb;19(2):656-72
17307930
Mol Cell Proteomics. 2007 Feb;6(2):193-206
17092930
Biochim Biophys Acta. 2002 Dec 2;1556(2-3):265-72
12460685
FEMS Microbiol Lett. 1998 Dec 15;169(2):323-30
9868777
J Bacteriol. 1988 Nov;170(11):5018-26
3141374
Plant Cell. 2006 Apr;18(4):992-1007
16531492
Proc Natl Acad Sci U S A. 1985 Oct;82(20):6903-7
3931080
J Bacteriol. 2000 Nov;182(22):6523-4
11053401
J Biol Chem. 2004 May 28;279(22):22866-74
15033974
Eur J Biochem. 1988 Nov 1;177(2):403-10
3191923
Plant Physiol. 1988 Oct;88(2):497-505
16666334
FEBS Lett. 2003 Aug 14;549(1-3):52-6
12914924
FEBS Lett. 2005 Apr 25;579(11):2289-93
15848160
Plant Physiol. 2005 Jul;138(3):1577-85
15908597
Biochemistry. 2005 Aug 16;44(32):10846-53
16086587
FEMS Microbiol Lett. 2003 Oct 24;227(2):255-62
14592717
Plant Physiol. 1983 Oct;73(2):250-6
16663203
Plant Physiol. 2004 Jan;134(1):470-81
14730074
Nat Protoc. 2006;1(1):16-22
17406207
Plant Physiol. 1985 Sep;79(1):290-5
16664388
Biochim Biophys Acta. 2004 Nov 4;1659(1):100-4
15511532
Appl Environ Microbiol. 2001 Nov;67(11):5247-53
11679352
Photosynth Res. 2007 Jul-Sep;93(1-3):17-25
17375369
FEBS Lett. 2005 Jun 13;579(15):3253-7
15943969
Plant Physiol. 1993 Nov;103(3):893-902
8022940
Photosynth Res. 2008 Sep;97(3):195-204
18720026
Physiol Plant. 2004 Jan;120(1):36-50
15032875
Plant Physiol. 1982 Jun;69(6):1376-81
16662407
Plant Physiol. 1996 Aug;111(4):1293-1298
12226362
Microbiol Mol Biol Rev. 1998 Sep;62(3):667-83
9729605
Arch Microbiol. 1999 Dec;172(6):377-86
10591847
Biochemistry. 2004 Aug 17;43(32):10308-13
15301529
FEBS Lett. 2004 Sep 10;574(1-3):126-30
15358552
Biochemistry (Mosc). 2007 Oct;72(10):1127-35
18021070
J Biol Chem. 1995 Dec 15;270(50):30181-6
8530427
FEBS Lett. 2000 Nov 24;485(2-3):173-7
11094162
Photochem Photobiol. 2001 Sep;74(3):431-7
11594057
Plant Physiol. 2006 Aug;141(4):1436-45
16798943
Nature. 2001 Aug 16;412(6848):745-8
11507644
Physiol Plant. 2002 Oct;116(2):255-263
12354203
Nature. 2001 Aug 16;412(6848):743-5
11507643
FEMS Microbiol Lett. 2001 Apr 1;197(1):123-9
11287157
Plant Physiol. 2008 Jul;147(3):1239-50
18502976
Biochemistry. 2003 Apr 8;42(13):3893-903
12667080