title: Novel Design for Centrifugal Countercurrent Chromatography: II. Studies on Novel Geometries of Zigzag Toroidal Tubing.
abstract: The toroidal column using a zigzag pattern has been improved in both retention of the stationary phase and peak resolution. To further improve the retention of stationary phase and peak resolution, a series of novel geometric designs of tubing (plain, mid-clamping, flattened and flat-twisted tubing) was evaluated their performance in CCC. The results showed that the tubing which was flattened vertically against centrifugal force (vert-flattened tubing) produced the best peak resolution among them. Using vert-flattened tubing a series of experiments was performed to study the effects of column capacity and sample size. The results indicated that a 0.25 ml capacity column is ideal for analysis of small amount samples.
authors.affiliation: Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 8N230, Bethesda, MD 20892, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Haji Akber
authors.initials: HA
authors.lastname: Aisa
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
references.citation: J Liq Chromatogr Relat Technol. 2009 Jan 1;32(14):2030-2042
references.pmid: 20046954
references.citation: J Chromatogr A. 2009 May 8;1216(19):4193-200
references.pmid: 19062024
references.citation: Anal Chem. 1995 Sep 1;67(17):3069-74
references.pmid: 8779424
references.citation: J Chromatogr A. 2009 Jul 3;1216(27):5265-71
references.pmid: 19486987
references.citation: J Liq Chromatogr Relat Technol. 2009;32(4):560-566
references.pmid: 20046940
references.citation: J Chromatogr A. 2001 Jul 13;922(1-2):355-8
references.pmid: 11486882
references.citation: J Liq Chromatogr Relat Technol. 2008 Jan 1;31(19):3012-3019
references.pmid: 20351801