title: A method for the isolation and purification of mouse peripheral blood monocytes.
abstract: Studies in mice have advanced our understanding of the contribution of monocytes to inflammation and immunity. However, the lack of a straightforward and reliable method for the isolation of mouse blood monocytes remains a hurdle. Here we describe a fast and easy method for isolating monocytes from mouse blood based on immuno-magnetic labeling. By negative selection we were able to isolate enriched fractions (>50% purity) of unlabeled monocytes. A subsequent positive selection step resulted in purities of > or =90% viable and functional monocytes. This method is of general use for studying mouse monocyte biology.
authors.affiliation: Department for Molecular Biomedical Research, VIB, Ghent, Belgium.
authors.forename: Erica
authors.initials: E
authors.lastname: Houthuys
authors.forename: Kiavash
authors.initials: K
authors.lastname: Movahedi
authors.forename: Patrick
authors.initials: P
authors.lastname: De Baetselier
authors.forename: Jo A
authors.initials: JA
authors.lastname: Van Ginderachter
authors.forename: Peter
authors.initials: P
authors.lastname: Brouckaert
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D018189:Immunomagnetic Separation / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
chemical_list: D015703:Antigens, CD