title: Myoglobin redox form stabilization by compartmentalized lactate and malate dehydrogenases.
abstract: The purpose of this study was to assess the ability of mitochondrial and cytoplasmic malate dehydrogenase present in postrigor bovine skeletal muscle to use malate as a substrate for reduced nicotinamide adenine dinucleotide (NADH) regeneration and metmyoglobin (MMb) reduction via the malate-NAD(+)-MMb system. Furthermore, addition of lactate to beef mitochondrial and cytoplasmic isolates was evaluated to determine whether interactions between malate and lactate increased MMb reduction. Addition of malate to isolated beef mitochondrial and cytoplasmic isolates at pH 7.2 increased (p < 0.05) MMb reduction. MMb reduction resulting from addition of malate and lactate was equal to or greater than MMb reduction resulting from malate alone. This suggests that a combination of mitochondrial (malate) and cytoplasmic (lactate) factors can be used to regenerate the post-mortem pool of NADH, resulting in metmyoglobin reduction and meat color stabilization.
authors.affiliation: Department of Animal Sciences and Industry, Weber Hall, Kansas State University, Manhattan, Kansas 66506, USA.
authors.forename: Anand
authors.initials: A
authors.lastname: Mohan
authors.forename: Melvin C
authors.initials: MC
authors.lastname: Hunt
authors.forename: S
authors.initials: S
authors.lastname: Muthukrishnan
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Barstow
authors.forename: Terry A
authors.initials: TA
authors.lastname: Houser
mesh_terms: D000818:Animals; D002417:Cattle; D003593:Cytoplasm / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D004795:Enzyme Stability; D006736:Horses; D019344:Lactic Acid / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D008928:Mitochondria / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D009211:Myoglobin / Q000737:chemistry / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D013379:Substrate Specificity
chemical_list: D009211:Myoglobin; D009243:NAD; D019344:Lactic Acid; D008291:Malate Dehydrogenase