title: Separation and purification of triterpene saponins from roots of Radix phytolaccae by high-speed countercurrent chromatography coupled with evaporative light scattering detection.
abstract: Coupled with evaporative light scattering detection, high-speed countercurrent chromatography was successfully applied for the first time to separation and purification of four triterpene saponins including esculentoside A, B, C and D from roots of Radix Phytolaccae. The separation was performed with an optimized two-phase solvent system composed of chloroform-methanol-water (4:4:2, v/v) using the lower phase as the mobile phase at a flow rate of 1.5 ml/min,. From 150 mg of crude extract 46.3 mg of esculentoside A, 21.8 mg of esculentoside B, 7.3 mg of esculentoside C, and 13.6 mg of esculentoside D were obtained at purities of 96.7%, 99.2%, 96.5% and 97.8%, respectively, as determined by HPLC analysis. The structures of the four triterpene saponins were identified by ESI-MS,(1)H NMR and (13)C NMR.
authors.affiliation: Biomedicine Key Laboratory of Shaanxi Procvince, Northwest Univesity, Xi'an, 710069, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Qianliang
authors.initials: Q
authors.lastname: Chen
authors.forename: Daowan
authors.initials: D
authors.lastname: Lai
authors.forename: Wenji
authors.initials: W
authors.lastname: Sun
authors.forename: Tianyou
authors.initials: T
authors.lastname: Zhang
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000073559:Caryophyllales / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification; D014315:Triterpenes / Q000032:analysis / Q000302:isolation & purification; D003377:Countercurrent Distribution / Q000379:methods; D009828:Oleanolic Acid / Q000031:analogs & derivatives
chemical_list: D012503:Saponins; D014315:Triterpenes; C000717998:esculentoside B; C076297:esculentoside A; D009828:Oleanolic Acid
references.citation: Planta Med. 1989 Dec;55(6):551-2
references.pmid: 2482514
references.citation: J Nat Prod. 2008 Jan;71(1):35-40
references.pmid: 18177012
references.citation: J Chromatogr A. 2003 Aug 8;1008(2):173-80
references.pmid: 12967182
references.citation: Yao Xue Xue Bao. 1984 Nov;19(11):825-9
references.pmid: 6544549
references.citation: J Chromatogr A. 2007 Jan 19;1139(2):254-62
references.pmid: 17150224
references.citation: Planta Med. 1991 Apr;57(2):162-4
references.pmid: 17226142
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 1;852(1-2):679-83
references.pmid: 17442637
references.citation: J Chromatogr A. 2009 May 8;1216(19):4258-62
references.pmid: 19344906
references.citation: Planta Med. 1990 Jun;56(3):301-3
references.pmid: 2392493
references.citation: Yao Xue Xue Bao. 1990;25(10):745-9
references.pmid: 2099588
references.citation: J Chromatogr A. 2005 Feb 18;1065(2):145-68
references.pmid: 15782961
references.citation: Talanta. 2007 Mar 30;71(5):1873-6
references.pmid: 19071536