Genes encoding carbocycle-forming enzymes involved in aminoglycoside biosynthesis in deep-sea environmental DNA.
We obtained 19 individual DNA fragments encoding 2-deoxy-scyllo-inosose synthase involved in the biosynthesis of aminoglycoside antibiotics from deep-sea sediments of the Pacific Ocean. Compared with genes from land-based environmental DNA, they showed low diversity. Combined with our previous study concerning the discovery of other aminoglycoside-biosynthetic genes from the same deep-sea samples, we suggest the importance of exploration of multiple biosynthetic genes to determine the diversity of aminoglycoside producers. We found that the deep sea is a useful source for screening of these genes.
Tamegai
Hideyuki
H
Department of Chemistry, College of Humanities and Sciences, Nihon University, Tokyo, Japan. htamegai@chs.nihon-u.ac.jp
Kanda
Yumi
Y
Kato
Chiaki
C
D000595:Amino Acid Sequence; D000617:Aminoglycosides / Q000096:biosynthesis*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001419:Bacteria / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D008190:Lyases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009792:Oceans and Seas
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D008190:Lyases; C085631:2-deoxy-scyllo-inosose synthase