title: Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identities.
abstract: BACKGROUND
  The term endothelial progenitor cells (EPCs) is currently used to refer to cell populations which are quite dissimilar in terms of biological properties. This study provides a detailed molecular fingerprint for two EPC subtypes: early EPCs (eEPCs) and outgrowth endothelial cells (OECs).
  
  
  METHODS
  Human blood-derived eEPCs and OECs were characterised by using genome-wide transcriptional profiling, 2D protein electrophoresis, and electron microscopy. Comparative analysis at the transcript and protein level included monocytes and mature endothelial cells as reference cell types.
  
  
  RESULTS
  Our data show that eEPCs and OECs have strikingly different gene expression signatures. Many highly expressed transcripts in eEPCs are haematopoietic specific (RUNX1, WAS, LYN) with links to immunity and inflammation (TLRs, CD14, HLAs), whereas many transcripts involved in vascular development and angiogenesis-related signalling pathways (Tie2, eNOS, Ephrins) are highly expressed in OECs. Comparative analysis with monocytes and mature endothelial cells clusters eEPCs with monocytes, while OECs segment with endothelial cells. Similarly, proteomic analysis revealed that 90% of spots identified by 2-D gel analysis are common between OECs and endothelial cells while eEPCs share 77% with monocytes. In line with the expression pattern of caveolins and cadherins identified by microarray analysis, ultrastructural evaluation highlighted the presence of caveolae and adherens junctions only in OECs.
  
  
  CONCLUSIONS
  This study provides evidence that eEPCs are haematopoietic cells with a molecular phenotype linked to monocytes; whereas OECs exhibit commitment to the endothelial lineage. These findings indicate that OECs might be an attractive cell candidate for inducing therapeutic angiogenesis, while eEPC should be used with caution because of their monocytic nature.
authors.affiliation: Centre for Vision & Vascular Science, School of Medicine, Dentistry & BioMedical Science, Queen's University Belfast, Belfast, BT12 6BA, UK.
authors.forename: Reinhold J
authors.initials: RJ
authors.lastname: Medina
authors.forename: Christina L
authors.initials: CL
authors.lastname: O'Neill
authors.forename: Mark
authors.initials: M
authors.lastname: Sweeney
authors.forename: Jasenka
authors.initials: J
authors.lastname: Guduric-Fuchs
authors.forename: Tom A
authors.initials: TA
authors.lastname: Gardiner
authors.forename: David A
authors.initials: DA
authors.lastname: Simpson
authors.forename: Alan W
authors.initials: AW
authors.lastname: Stitt
mesh_terms: D000328:Adult; D015820:Cadherins / Q000378:metabolism; D022461:Caveolins / Q000378:metabolism; D019070:Cell Lineage; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D042783:Endothelial Cells / Q000378:metabolism* / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D020869:Gene Expression Profiling; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D025341:Principal Component Analysis; D015398:Signal Transduction; D013234:Stem Cells / Q000145:classification / Q000378:metabolism*
chemical_list: D015820:Cadherins; D022461:Caveolins
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