Ultraviolet irradiation induces autofluorescence enhancement via production of reactive oxygen species and photodecomposition in erythrocytes.
Ultraviolet (UV) light has a significant influence on human health. In this study, human erythrocytes were exposed to UV light to investigate the effects of UV irradiation (UVI) on autofluorescence. Our results showed that high-dose continuous UVI enhanced erythrocyte autofluorescence, whereas low-dose pulsed UVI alone did not have this effect. Further, we found that H(2)O(2), one type of reactive oxygen species (ROS), accelerated autofluorescence enhancement under both continuous and pulsed UVI. In contrast, continuous and pulsed visible light did not result in erythrocyte autofluorescence enhancement in the presence or absence of H(2)O(2). Moreover, NAD(P)H had little effect on UVI-induced autofluorescence enhancement. From these studies, we conclude that UVI-induced erythrocyte autofluorescence enhancement via both UVI-dependent ROS production and photodecomposition. Finally, we present a theoretical study of this autofluorescence enhancement using a rate equation model. Notably, the results of this theoretical simulation agree well with the experimental data further supporting our conclusion that UVI plays two roles in the autofluorescence enhancement process.
Wu
Xian
X
The Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, TEDA Applied Physics School and School of Physics, Nankai University, Tianjin 300457, China.
Pan
Leiting
L
Wang
Zhenhua
Z
Liu
Xiaoli
X
Zhao
Dan
D
Zhang
Xinzheng
X
Rupp
Romano A
RA
Xu
Jingjun
J
D002478:Cells, Cultured; D004912:Erythrocytes / Q000378:metabolism / Q000528:radiation effects*; D005453:Fluorescence; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014466:Ultraviolet Rays
D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide