title: Branched arabino-oligosaccharides isolated from sugar beet arabinan.
abstract: Sugar beet arabinan consists of an alpha-(1,5)-linked backbone of L-arabinosyl residues, which can be either single or double substituted with alpha-(1,2)- and/or alpha-(1,3)-linked L-arabinosyl residues. Neutral branched arabino-oligosaccharides were isolated from sugar beet arabinan by enzymatic degradation with mixtures of pure and well-defined arabinohydrolases from Chrysosporium lucknowense followed by fractionation based on size and analysis by MALDI-TOF MS and HPAEC. Using NMR analysis, two main series of branched arabino-oligosaccharides have been identified, both having an alpha-(1,5)-linked backbone of L-arabinosyl residues. One series carries single substituted alpha-(1,3)-linked L-arabinosyl residues at the backbone, whereas the other series consists of a double substituted alpha-(1,2,3,5)-linked arabinan structure within the molecule. The structures of eight such branched arabino-oligosaccharides were established.
authors.affiliation: Wageningen University, Laboratory of Food Chemistry, Wageningen, The Netherlands.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Westphal
authors.forename: Stefan
authors.initials: S
authors.lastname: Kühnel
authors.forename: Pieter
authors.initials: P
authors.lastname: de Waard
authors.forename: Sandra W A
authors.initials: SW
authors.lastname: Hinz
authors.forename: Henk A
authors.initials: HA
authors.lastname: Schols
authors.forename: Alphons G J
authors.initials: AG
authors.lastname: Voragen
authors.forename: Harry
authors.initials: H
authors.lastname: Gruppen
mesh_terms: D001089:Arabinose / Q000737:chemistry*; D027461:Beta vulgaris / Q000737:chemistry; D002240:Carbohydrate Sequence; D002912:Chrysosporium / Q000201:enzymology; D019281:Dimerization; D006867:Hydrolases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides; C030080:araban; D001089:Arabinose; D006867:Hydrolases