title: Diagnostic approach to drug-screening tests for fatal diabetic ketoacidosis: forensic autopsy of a methamphetamine abuser.
abstract: To diagnose the cause of death in autopsy cases, systematic examinations, such as macroscopic, pathological, biochemical, and toxicological are important. In this case report, drug examinations also gave very useful information to diagnose the cause of death, fatal diabetic ketoacidosis (DKA). A female methamphetamine abuser in her forties was found dead lying on a hotel bed. Diagnosing her cause of death was difficult only from the macroscopic findings because there was no fatal and/or serious injury or disease. On toxicological examination, acetone was detected at a high concentration (682 microg/mL in blood, 887 microg/mL in urine) using gas chromatography (GC). Using gas chromatography-mass spectrometry (GC-MS), methamphetamine was detected in the blood, urine, hair, and visceral organs; however, these concentrations were low. At the same time, GC-MS examination revealed a high glucose peak. From the results of the biochemical examination of urine, acetoacetic acid was 1940 micromol/L, beta-hydroxybutyric acid was 14,720 micromol/L, and glucose was 4620 mg/dL. Histologically, Langerhans' islets in the pancreas were fibrotic and atrophic, and no insulin-immunoreactive cells were observed. The subsequent police investigation also revealed that she had contracted diabetes mellitus type 1; therefore, we concluded that her cause of death was DKA, due to a lack of insulin injection.
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Jonan-ku, Fukuoka, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kashiwagi
authors.forename: Kenji
authors.initials: K
authors.lastname: Hara
authors.forename: Zhao
authors.initials: Z
authors.lastname: Liu
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Kageura
authors.forename: Aya
authors.initials: A
authors.lastname: Matsusue
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sugimura
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kubo
mesh_terms: D000096:Acetone / Q000097:blood / Q000652:urine; D000328:Adult; D001344:Autopsy; D002423:Cause of Death; D016883:Diabetic Ketoacidosis / Q000175:diagnosis*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008694:Methamphetamine / Q000097:blood* / Q000652:urine*; D019966:Substance-Related Disorders
chemical_list: D000096:Acetone; D008694:Methamphetamine