title: Determination of salsolinol, norsalsolinol, and twenty-one biogenic amines using micellar electrokinetic capillary chromatography-electrochemical detection.
abstract: Micellar electrokinetic chromatography coupled to amperometric electrochemical detection was used to resolve and then quantify biogenic amines and metabolites within the fruit fly Drosophila melanogaster. A new separation scheme was devised to allow resolution of 24 compounds of interest. This was accomplished by precisely controlling the amount of base added to the background buffer, optimizing the resolution of the separation, and then calculating the pH. Here we focused on measurements of six of the analytes that are thought to be involved in the response to alcohol, dopamine, salsolinol, norsalsolinol, N-acetyloctopamine, octopamine, and N-acetyldopamine. These were identified and quantified within the fly head. We believe that the identification of salsolinol and norsalsolinol in the fly brain is novel.
authors.affiliation: Department of Chemistry, The Pennsylvania State University, PA, USA.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Kuklinski
authors.forename: E Carina
authors.initials: EC
authors.lastname: Berglund
authors.forename: Johan
authors.initials: J
authors.lastname: Engelbreksson
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Ewing
mesh_terms: D000818:Animals; D001679:Biogenic Amines / Q000032:analysis* / Q000737:chemistry; D001881:Borates / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D029721:Drosophila Proteins / Q000032:analysis / Q000737:chemistry; D004331:Drosophila melanogaster / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007546:Isoquinolines / Q000032:analysis* / Q000737:chemistry; D008297:Male; D009420:Nervous System / Q000737:chemistry; D012044:Regression Analysis; D012490:Salsoline Alkaloids / Q000032:analysis* / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D001679:Biogenic Amines; D001881:Borates; D029721:Drosophila Proteins; D007546:Isoquinolines; D012490:Salsoline Alkaloids; D012967:Sodium Dodecyl Sulfate; C036617:salsolinol; C020784:norsalsolinol
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