title: Comparative studies on lectin-carbohydrate interactions in low and high density homo- and heteroglycoclusters.
abstract: A versatile synthetic procedure to construct series of high- and low-density homo- and heteroglycoclusters is reported. The binding properties of these synthetic multivalent glycoconjugates to concanavalin A (Con A), a model lectin, have been assessed by using a range of competitive and non-competitive binding assays including enzyme-linked lectin assays (ELLA), isothermal titration microcalorimetry (ITC) and surface plasmon resonance (SPR). In all cases, highly dense glycoclusters showed a substantial amplification of the lectin-binding strength in comparison with low-density counterparts. Interestingly, highly-dense glycoligand presentations, regardless of their homo- or heteroglycoligand pattern, furnished similar Con A binding properties, supporting the existence of a synergic effect (heterocluster effect) due to secondary interactions of "non-active" structural motifs in the presence of a certain density of "active" glycoligands.
authors.affiliation: Departamento de Química Orgánica, Facultad de Química, Universidad de Sevilla, Apartado 1203, E-41012 Sevilla, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Gómez-García
authors.forename: Juan M
authors.initials: JM
authors.lastname: Benito
authors.forename: Ricardo
authors.initials: R
authors.lastname: Gutiérrez-Gallego
authors.forename: Alfredo
authors.initials: A
authors.lastname: Maestre
authors.forename: Carmen Ortiz
authors.initials: CO
authors.lastname: Mellet
authors.forename: José M García
authors.initials: JM
authors.lastname: Fernández
authors.forename: José L Jiménez
authors.initials: JL
authors.lastname: Blanco
mesh_terms: D001665:Binding Sites; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000138:chemical synthesis / Q000737:chemistry; D003208:Concanavalin A / Q000378:metabolism*; D057075:Enzyme Assays; D006001:Glycoconjugates / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020349:Surface Plasmon Resonance
chemical_list: D002241:Carbohydrates; D006001:Glycoconjugates; D037102:Lectins; D003208:Concanavalin A