[Effect of ultrasound microbubble carrying herpes simplex virus thymidine kinase on hepatocellular carcinoma in mice].
OBJECTIVE
To observe the effect of ultrasound microbubble carrying herpes simplex virus thymidine kinase hepatocellular carcinoma in mice.


METHODS
Kunming mice were inoculated subcutaneously with H22 tumor cells. 40 male mice bearing subcutaneous hepatoma were randomized into 4 groups: PBS (group A), HSV1-TK (group B), HSV1-TK (group C), and microbubble carrying HSV1-TK (group D) were injected into the tail vein every 3 days. Mice in group C and D were exposed to ultrasound. The expression of TK protein was detected by western blot. Ganciclovir (GCV) was intraperitoneally injected at a dose of 100 mg x kg (-1) x d(-1) in group B, group C and group D. The tumor size was measured every 2 days.


RESULTS
TK gene could be injected precisely into hepatocellular carcinoma with ultrasound monitor, and the expression of TK protein was found in all 4 groups. Expression in group D was higher than others (P < 0.05). The rate of tumor growth inhibition were 0 in group A, 3.90%+/-1.80% in group B, 22.70%+/-2.86% in group C, 41.25%+/-3.20% in group D (group B vs group C, P < 0.05; group D vs group C, P < 0.05; group D vs group B, P < 0.05).


CONCLUSIONS
Ultrasound microbubble not only improve target gene therapy, but also enhance transfection efficiency.
Zhou
Shi-ji
SJ
Department of Hepatobiliary Surgery, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
Liu
Chan-an
CA
Gong
Jian-ping
JP
Liu
Zuo-jin
ZJ
Tang
Yong
Y
Li
Sheng-wei
SW
Xu
Yue
Y
Ai
Zhi-guo
ZG
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000628:therapy*; D045744:Cell Line, Tumor; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy; D008113:Liver Neoplasms / Q000628:therapy*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D045423:Microbubbles; D018139:Simplexvirus / Q000235:genetics / Q000378:metabolism; D013937:Thymidine Kinase / Q000235:genetics*; D016896:Treatment Outcome; D014465:Ultrasonics
D013937:Thymidine Kinase