title: [Molecular and structural-biological analysis of Nicotiana plumbaginifolia mutants for identification of the site of beta-tubulins interaction with dinitroanilines and phosphorotioamidates].
abstract: The identification of point mutation locations on beta-tubulin molecules of amiprophosmethyl- and trifluralin-resistant Nicotiana plumbaginifolia lines have described in the work. It was shown that in the first case this mutation is connected with the substitution ofserine residue on proline in position 248; in the second case--with the substitution of phenilalanine on serine in position 317 of beta-tubulin amino acid sequence. Three-dimensional models of beta-tubulin molecule from Chlamydomonas with well-known location of mutations conferring dinitroaniline- and phosphorotioamidate resistance (substitution of lysine residue to methionine on position 350), and beta-tubulin from Nicotiana plumbaginifolia have been reconstructed. On the basis of analysis of site of interaction with dinitroanilines and phosphorotioamides on Chlamydomonas beta-tubulin molecule it was concluded that the revealed mutations on Nicotiana plumbaginifolia beta-tubulin affect amino acid residues participating in formation of this site.
authors.forename: A I
authors.initials: AI
authors.lastname: Emets
authors.forename: U V
authors.initials: UV
authors.lastname: Baiard
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Nyporko
authors.forename: G A
authors.initials: GA
authors.lastname: Swire-Clark
authors.forename: Ia B
authors.initials: IaB
authors.lastname: Blium
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000814:Aniline Compounds / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites; D015139:Blotting, Southern; D016825:Chlamydomonas reinhardtii / Q000187:drug effects / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D053209:Herbicide Resistance / Q000235:genetics; D006540:Herbicides / Q000737:chemistry / Q000494:pharmacology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009946:Organothiophosphorus Compounds / Q000737:chemistry / Q000494:pharmacology*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics*; D014404:Tubulin / Q000235:genetics*
chemical_list: D000814:Aniline Compounds; D018744:DNA, Plant; D006540:Herbicides; D009946:Organothiophosphorus Compounds; D014404:Tubulin