A quantitative investigation of fucosylated serum glycoproteins with application to esophageal adenocarcinoma.
Although glycoproteomic studies provide unique opportunities for cancer research, it has been necessary to develop specific methods for analysis of oncologically interesting glycoproteins. We describe a general, multimethodological approach for quantitative glycoproteomic analysis of fucosylated glycoproteins in human blood serum. A total of 136 putative fucosylated glycoproteins were identified with very high confidence in three clinically relevant sample pools (N=5 for each), with a mean CV of 3.1% observed for replicate analyses. Two samples were collected from subjects diagnosed with esophagus disease states, high-grade dysplasia plus esophageal adenocarcinoma, while the third sample was representative of a disease-free condition. Some glycoproteins, observed to be significantly upregulated in esophageal adenocarcinoma, i.e. more than twofold higher than in the disease-free condition, are briefly discussed. Further investigation will be necessary to validate these findings; however, the method itself is demonstrated to be an effective tool for quantitative glycoproteomics of clinical samples.
Mann
Benjamin
B
Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
Madera
Milan
M
Klouckova
Iveta
I
Mechref
Yehia
Y
Dobrolecki
Lacey E
LE
Hickey
Robert J
RJ
Hammoud
Zane T
ZT
Novotny
Milos V
MV
D000230:Adenocarcinoma / Q000097:blood*; D024042:Collagen Type I / Q000032:analysis / Q000097:blood; D000090602:Collagen Type I, alpha 1 Chain; D004938:Esophageal Neoplasms / Q000097:blood*; D060752:Fetuin-B; D005643:Fucose / Q000097:blood*; D006023:Glycoproteins / Q000032:analysis / Q000097:blood*; D006801:Humans; D037102:Lectins / Q000737:chemistry; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D014357:Trypsin / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000032:analysis / Q000378:metabolism
D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; C426245:EMILIN2 protein, human; C504687:FETUB protein, human; D060752:Fetuin-B; D006023:Glycoproteins; D037102:Lectins; D010446:Peptide Fragments; D020543:Proteome; D000509:alpha-Fetoproteins; C027112:fucose-binding lectin; C064549:lectin, Aleuria aurantia; D005643:Fucose; D014357:Trypsin
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