Clathrin assembly lymphoid myeloid leukemia-AF10-positive acute leukemias: a report of 2 cases with a review of the literature.
The translocation t(10;11)(p13;q14q21) has been found to be recurrent in acute lymphoblastic and myeloid leukemias, and results in the fusion of the clathrin assembly lymphoid myeloid leukemia (CALM) gene with the AF10 gene; these genes are present on chromosomes 11 and 10, respectively. Because the CALM-AF10 rearrangement is a rare chromosomal abnormality, it is not included in routine molecular tests for acute leukemia. Here, we describe the cases of 2 patients with the CALM-AF10 fusion gene. The first patient (case 1) was diagnosed with T-cell ALL, and the second patient (case 2) was diagnosed with AML. Both patient samples showed expression of the homeobox A gene cluster and the histone methyltransferase hDOT1L, which suggests that they mediate leukemic transformation in CALM-AF10-positive and mixed-lineage leukemia-AF10-positive leukemias. Both patients achieved complete remission after induction chemotherapy. The first patient (case 1) relapsed after double-unit cord blood transplantation; there was no evidence of relapse in the second patient (case 2) after allogenic peripheral blood stem cell transplantation. Since CALM-AF10- positive leukemias have been shown to have poor prognosis with conventional therapy, molecular tests for CALM-AF10 rearrangement would be necessary to detect minimal residual disease during follow-up.
Huh
Ji Young
JY
Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea.
Chung
Soie
S
Oh
Doyeun
D
Kang
Myung Seo
MS
Eom
Hyeon Seok
HS
Cho
Eun Hae
EH
Han
Mi Hwa
MH
Kong
Sun Young
SY
D000293:Adolescent; D000328:Adult; D001853:Bone Marrow / Q000473:pathology; D002879:Chromosomes, Human, Pair 10; D002880:Chromosomes, Human, Pair 11; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D008297:Male; D034041:Monomeric Clathrin Assembly Proteins / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D012008:Recurrence; D014157:Transcription Factors / Q000235:genetics*; D014178:Translocation, Genetic
D018398:Homeodomain Proteins; C092513:MLLT10 protein, human; D034041:Monomeric Clathrin Assembly Proteins; D015514:Oncogene Proteins, Fusion; C439003:PICALM protein, human; D014157:Transcription Factors; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase