Quantitative analysis for the effect of plant oil and fatty acid on Tuber melanosporum culture by uniform design combined with partial least squares regression.
Uniform design and partial least squares regression were adopted to quantitatively analyze the effects of plant oil and fatty acid as well as their addition amount and addition time on the performance of Tuber melanosporum submerged fermentation. The regression models showed the optimal scheme was the addition of 1.2 mL soybean oil on day 9, which was validated by experiment. The maximal biomass of 25.89 +/- 1.01 g/L and extracellular polysaccharides (EPS) production of 6.51 +/- 0.68 g/L were obtained, which were enhanced by 18.5% and 86%, respectively. Palmitic acid was identified to be the key component to stimulate the cell growth and EPS biosynthesis, and stearic acid was beneficial for the production of intracellular polysaccharides. Not only the biomass but also EPS production obtained in this work are the highest reported in the batch fermentation of truffle.
Liu
Rui-Sang
RS
Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
Tang
Ya-Jie
YJ
D001203:Ascomycota / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D005227:Fatty Acids / Q000494:pharmacology*; D005285:Fermentation; D006131:Growth Inhibitors / Q000494:pharmacology*; D019308:Palmitic Acid / Q000032:analysis / Q000494:pharmacology; D011134:Polysaccharides / Q000378:metabolism; D013024:Soybean Oil / Q000737:chemistry / Q000494:pharmacology*; D013229:Stearic Acids / Q000032:analysis / Q000494:pharmacology; D013997:Time Factors
D005227:Fatty Acids; D006131:Growth Inhibitors; D011134:Polysaccharides; D013229:Stearic Acids; D019308:Palmitic Acid; C031183:stearic acid; D013024:Soybean Oil