title: Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry.
abstract: The ability to measure plant hormones quantitatively is important as plant hormones regulate plant growth, development and response to biotic and abiotic cues. In this protocol, we describe the quantitative analysis of major plant hormones from crude plant extracts. Plant hormones are determined using reverse-phase liquid chromatography-tandem mass spectrometry with multiple reaction monitoring. The method provides quantification of most major plant hormones in a single run from 50 mg of fresh plant tissue. Extraction and quantitative analysis of 40 samples takes 2-3 d.
authors.affiliation: Department of Biology, University of Missouri, St. Louis, MO, USA. xpan@solae.com
authors.forename: Xiangqing
authors.initials: X
authors.lastname: Pan
authors.forename: Ruth
authors.initials: R
authors.lastname: Welti
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D010937:Plant Growth Regulators / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010936:Plant Extracts; D010937:Plant Growth Regulators
references.citation: Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10552-7
references.pmid: 12874387
references.citation: Ann Bot. 2006 May;97(5):883-93
references.pmid: 16473866
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Sep 15;877(26):2806-13
references.pmid: 19427277
references.citation: Phytochemistry. 2008 May;69(8):1773-81
references.pmid: 18367217
references.citation: J Chromatogr A. 2003 Aug 22;1010(1):75-85
references.pmid: 14503817
references.citation: Curr Opin Plant Biol. 1998 Oct;1(5):404-11
references.pmid: 10066616
references.citation: Planta. 2002 Nov;216(1):44-56
references.pmid: 12430013
references.citation: Plant Cell. 2008 Mar;20(3):803-16
references.pmid: 18364466
references.citation: Biosci Biotechnol Biochem. 2005 Apr;69(4):778-83
references.pmid: 15849417
references.citation: J Agric Food Chem. 2005 Nov 2;53(22):8437-42
references.pmid: 16248534
references.citation: Plant Physiol. 2001 Dec;127(4):1845-53
references.pmid: 11743128
references.citation: Anal Biochem. 2003 Jan 15;312(2):242-50
references.pmid: 12531212
references.citation: Curr Opin Plant Biol. 2001 Oct;4(5):387-91
references.pmid: 11597495
references.citation: Nat Protoc. 2007;2(6):1350-5
references.pmid: 17545972
references.citation: Anal Biochem. 2004 Jun 15;329(2):324-33
references.pmid: 15158494
references.citation: Anal Chim Acta. 2009 Aug 26;648(2):215-25
references.pmid: 19646587
references.citation: J Chromatogr A. 2003 Apr 18;993(1-2):89-102
references.pmid: 12735441
references.citation: J Biol Chem. 2007 Jun 22;282(25):18116-18128
references.pmid: 17475618
references.citation: Cell. 2006 Aug 11;126(3):467-75
references.pmid: 16901781
references.citation: Phytochem Anal. 2002 Jul-Aug;13(4):228-34
references.pmid: 12184177
references.citation: Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10473-8
references.pmid: 9294235
references.citation: Anal Biochem. 1998 Mar 15;257(2):186-94
references.pmid: 9514789
references.citation: Plant Physiol Biochem. 2005 Apr;43(4):407-11
references.pmid: 15907693
references.citation: Plant J. 2003 Aug;35(3):405-17
references.pmid: 12887591
references.citation: Plant J. 2004 Sep;39(5):790-808
references.pmid: 15315639