Impaired water reabsorption in mice deficient in the type VI adenylyl cyclase (AC6).
Adenylyl cyclase (AC) type VI (AC6) is a calcium-inhibitable enzyme which produces cAMP upon stimulation. Herein, we characterized the specific role of AC6 in the kidneys using two AC6-knockout mouse lines. Immunohistochemical staining revealed that AC6 exists in the tubular parts of the nephron and collecting duct. Activities of AC evoked by forskolin or a selective agonist of the V2 vasopressin receptor were lower in the kidneys of AC6-null mice compared to those of wildtype mice. Results of a metabolic cage assay and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) showed for the first time that AC6 plays a critical role in regulating water homeostasis.
Chien
Chen-Li
CL
Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.
Wu
Yu-Shuo
YS
Lai
Hsing-Lin
HL
Chen
Yen-Hui
YH
Jiang
Si-Tse
ST
Shih
Chia-Ming
CM
Lin
Sui-Shan
SS
Chang
Chen
C
Chern
Yijuang
Y
D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D007150:Immunohistochemistry; D007685:Kidney Tubules, Collecting / Q000378:metabolism / Q000502:physiology; D008279:Magnetic Resonance Imaging; D051379:Mice; D018345:Mice, Knockout; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014867:Water / Q000378:metabolism*
D014867:Water; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; C121100:adenylyl cyclase 6