Comparative quantitation of proteome alterations induced by aging or immortalization in primary human fibroblasts and keratinocytes for clinical applications.
We monitor proteome changes of primary human skin fibroblasts and keratinocytes during cell culture and compare them to respective immortalized cell lines using stable isotope labeling by amino acids to address their suitability as cell models for clinical disease proteomics.
Sprenger
Adrian
A
Freiburg Institute for Advanced Studies, School of Life Science-LIFENET, University of Freiburg, Albertstr. 19, 79104 Freiburg, Germany.
Küttner
Victoria
V
Biniossek
Martin L
ML
Gretzmeier
Christine
C
Boerries
Melanie
M
Mack
Claudia
C
Has
Cristina
C
Bruckner-Tuderman
Leena
L
Dengjel
Jörn
J
D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*
D020543:Proteome