title: Identification of uricase as a potential target of plant thioredoxin: Implication in the regulation of nodule development.
abstract: During symbiotic nodule development in legume roots, early signaling events between host and rhizobia serve critical determinants for the proper onset of nodule morphogenesis, nitrogen fixation, and assimilation. Previously we isolated thioredoxin from soybean nodules as one of differentially expressed genes during nodulation and noted its positive role in nitrogen fixation. To identify the target proteins of thioredoxin in nodules, we used thioredoxin affinity chromatography followed by mass spectrometry. Nodulin-35, a subunit of uricase, was found to be a target of thioredoxin. Their interaction was confirmed by pull-down assay and by bimolecular fluorescent complementation. With an increased uricase activity observed also in the presence of thioredoxin, these results appear to implicate a novel role of thioredoxin in the regulation of enzyme activities involved in nodule development and nitrogen fixation.
authors.affiliation: Department of Biological Science, Sookmyung Women's University, Seoul, Republic of Korea.
authors.forename: Hui
authors.initials: H
authors.lastname: Du
authors.forename: Sunghan
authors.initials: S
authors.lastname: Kim
authors.forename: Kyoung Hee
authors.initials: KH
authors.lastname: Nam
authors.forename: Myung-Sok
authors.initials: MS
authors.lastname: Lee
authors.forename: Ora
authors.initials: O
authors.lastname: Son
authors.forename: Suk-Ha
authors.initials: SH
authors.lastname: Lee
authors.forename: Choong-Ill
authors.initials: CI
authors.lastname: Cheon
mesh_terms: D008565:Membrane Proteins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D053204:Root Nodules, Plant / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology; D013025:Glycine max / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology; D013559:Symbiosis; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D010940:Plant Proteins; C045625:nodulin; D013879:Thioredoxins