Human effector/initiator gene sets that regulate myometrial contractility during term and preterm labor.
OBJECTIVE
Distinct processes govern transition from quiescence to activation during term (TL) and preterm labor (PTL). We sought gene sets that are responsible for TL and PTL, along with the effector genes that are necessary for labor independent of gestation and underlying trigger.


METHODS
Expression was analyzed in term and preterm with or without labor (n=6 subjects/group). Gene sets were generated with logic operations.


RESULTS
Thirty-four genes were expressed similarly in PTL/TL but were absent from nonlabor samples (effector set); 49 genes were specific to PTL (preterm initiator set), and 174 genes were specific to TL (term initiator set). The gene ontogeny processes that comprise term initiator and effector sets were diverse, although inflammation was represented in 4 of the top 10; inflammation dominated the preterm initiator set.


CONCLUSIONS
TL and PTL differ dramatically in initiator profiles. Although inflammation is part of the term initiator and the effector sets, it is an overwhelming part of PTL that is associated with intraamniotic inflammation.
Weiner
Carl P
CP
Department of Obstetrics and Gynecology, University of Kansas School of Medicine, Kansas City, KS 66160, USA. cweiner@kumc.edu
Mason
Clifford W
CW
Dong
Yafeng
Y
Buhimschi
Irina A
IA
Swaan
Peter W
PW
Buhimschi
Catalin S
CS
D000328:Adult; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation / Q000235:genetics; D007743:Labor, Obstetric / Q000235:genetics*; D007752:Obstetric Labor, Premature / Q000235:genetics* / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D047929:Term Birth / Q000235:genetics*; D014590:Uterine Contraction / Q000235:genetics*; D055815:Young Adult
Biol Reprod. 2001 May;64(5):1526-34
11319161
J Soc Gynecol Investig. 2006 Jul;13(5):338-42
16814162
Biochem Biophys Res Commun. 2008 Dec 19;377(3):832-7
18948081
J Soc Gynecol Investig. 2003 Jul;10(5):252-64
12853086
Mol Hum Reprod. 2000 Dec;6(12):1141-5
11101697
Placenta. 2003 Apr;24 Suppl A:S33-46
12842412
J Immunol. 1991 Dec 1;147(11):3815-22
1940369
Reprod Biol Endocrinol. 2007 Nov 02;5:41
17980032
Dev Biol. 1997 Oct 15;190(2):178-90
9344537
Semin Fetal Neonatal Med. 2004 Dec;9(6):437-44
15691781
J Pharmacol Exp Ther. 1999 May;289(2):1022-30
10215683
Clin Diagn Lab Immunol. 2005 Jul;12(7):837-44
16002632
Reprod Sci. 2009 Jul;16(7):650-6
19351964
Am J Obstet Gynecol. 2006 Jun;194(6):1513-23
16731067
Genome Biol. 2005;6(2):R12
15693941
Inflamm Res. 2004 Nov;53(11):585-95
15693606
Reproduction. 2002 Sep;124(3):323-9
12201805
J Obstet Gynaecol Can. 2002 Nov;24(11):874-83
12417902
Biol Reprod. 2005 Mar;72(3):707-19
15509731
Placenta. 2002 Apr;23(4):257-73
11969336
Rev Reprod. 1996 May;1(2):89-96
9414445
Ann Med. 2001 Dec;33(9):571-8
11817651
J Biol Chem. 2001 Aug 24;276(34):31883-90
11427525
J Reprod Immunol. 2002 Oct-Nov;57(1-2):217-24
12385844
Pediatr Res. 2007 Mar;61(3):318-24
17314690
Am J Obstet Gynecol. 2006 Jul;195(1):275-87
16681987
Mol Hum Reprod. 2003 Nov;9(11):681-700
14561811
J Clin Endocrinol Metab. 2004 May;89(5):2463-9
15126578
Semin Reprod Med. 2007 Jan;25(1):21-39
17205421
Reproduction. 2008 Apr;135(4):569-79
18367515
Reproduction. 2002 Jul;124(1):1-17
12090913
PLoS Med. 2006 Jun;3(6):e169
16768543
Am J Obstet Gynecol. 2006 Mar;194(3):873-83
16522428
Am J Physiol Endocrinol Metab. 2005 Feb;288(2):E335-41
15454398
Am J Obstet Gynecol. 2003 Jul;189(1):181-8
12861160
Bratisl Lek Listy. 2007;108(4-5):174-8
17694800
Acta Physiol Scand. 1998 Sep;164(1):47-52
9777024
BJOG. 2003 Apr;110 Suppl 20:39-45
12763110
Semin Thromb Hemost. 1999;25(6):543-9
10632476
J Exp Med. 1991 Nov 1;174(5):1209-20
1940799
Mol Pharmacol. 2004 May;65(5):1051-9
15102932
Endocrinology. 2003 Jun;144(6):2254-65
12746283
Biol Reprod. 2003 Jun;68(6):2289-96
12606369
Am J Obstet Gynecol. 2005 Aug;193(2):404-13
16098862
Methods. 2001 Dec;25(4):402-8
11846609
J Soc Gynecol Investig. 2003 Sep;10(6):323-38
12969775
J Clin Endocrinol Metab. 2004 Aug;89(8):4149-52
15292362
BJOG. 2005 Feb;112(2):173-81
15663581
Mol Endocrinol. 2003 Aug;17(8):1454-69
12775764
J Soc Gynecol Investig. 2004 May;11(4):193-202
15120691
Br J Pharmacol. 2006 Apr;147(7):815-24
16415906
Am J Obstet Gynecol. 2003 Jan;188(1):84-91
12548200
Reproduction. 2006 May;131(5):837-50
16672349
PLoS Med. 2007 Jan;4(1):e18
17227133
Am J Obstet Gynecol. 1995 Oct;173(4):1223-7
7485325
N Engl J Med. 1999 Aug 26;341(9):660-6
10460818
J Clin Endocrinol Metab. 1998 Dec;83(12):4332-7
9851773
Semin Fetal Neonatal Med. 2006 Oct;11(5):317-26
16839830
Immunopharmacology. 1996 Jun;33(1-3):130-2
8856131