[Study on preparation of CIT microemulsion and its absorption in intestine].
OBJECTIVE
To prepare CIT microemulsion and to investigate its properities and the absorption character in rat intestine in situ.


METHODS
The optimum formulation of the blank microemulsion was selected by pseudo tertiary phase diagrams and the CIT microemulsion was prepared based on the blank microemulsion. The appearance, particle diameter distribution and Zeta potential were investigated by electron microscopy and grainsize analyzer. The entrapment efficiency was determined by sephadex column chromatography. An in situ single pass intestine perfusion method was used to investigate the intestinal absorption of CIT microemulsion. HPLC method for determination of CIT in the intestinal flux was established.


RESULTS
The formulation was OP-glycerol-water-ethylis oleas (3:3:4:2). CIT microemulsion in electron microscopy was consisted of small spherical drop. The mean diameter was 61.7 nm, Zeta potential was -46.3 mV. The Entrapment efficiency was (92.1 +/- 1.74)% (n=3). Both CIT and CIT microemulsion had been absorbed in general intestinal tract,and the absorption of CIT microemulsion was significantly high compared with CIT in duodenum, colon, jejunum and ileum (P < 0.01).


CONCLUSIONS
The preparation technology of CIT microemulsion was feasible,and the microemulsion system might improve the absorption of CIT in the intestinal tract.
Jia
Xiaobin
X
Department of Pharmaceutics, Jiangsu University, Zhenjiang 212013, China. jxiaobin2005@hotmail.com
Jia
Dongsheng
D
Shi
Feng
F
Xue
Jing
J
Sun
E
E
Lan
Xuelian
X
Tan
Xiaobin
X
D000818:Animals; D004339:Drug Compounding; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000493:pharmacokinetics; D004655:Emulsions / Q000737:chemistry; D007408:Intestinal Absorption; D023421:Models, Animal; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D004655:Emulsions