title: Impaired water reabsorption in mice deficient in the type VI adenylyl cyclase (AC6).
abstract: Adenylyl cyclase (AC) type VI (AC6) is a calcium-inhibitable enzyme which produces cAMP upon stimulation. Herein, we characterized the specific role of AC6 in the kidneys using two AC6-knockout mouse lines. Immunohistochemical staining revealed that AC6 exists in the tubular parts of the nephron and collecting duct. Activities of AC evoked by forskolin or a selective agonist of the V2 vasopressin receptor were lower in the kidneys of AC6-null mice compared to those of wildtype mice. Results of a metabolic cage assay and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) showed for the first time that AC6 plays a critical role in regulating water homeostasis.
authors.affiliation: Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Chen-Li
authors.initials: CL
authors.lastname: Chien
authors.forename: Yu-Shuo
authors.initials: YS
authors.lastname: Wu
authors.forename: Hsing-Lin
authors.initials: HL
authors.lastname: Lai
authors.forename: Yen-Hui
authors.initials: YH
authors.lastname: Chen
authors.forename: Si-Tse
authors.initials: ST
authors.lastname: Jiang
authors.forename: Chia-Ming
authors.initials: CM
authors.lastname: Shih
authors.forename: Sui-Shan
authors.initials: SS
authors.lastname: Lin
authors.forename: Chen
authors.initials: C
authors.lastname: Chang
authors.forename: Yijuang
authors.initials: Y
authors.lastname: Chern
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D007150:Immunohistochemistry; D007685:Kidney Tubules, Collecting / Q000378:metabolism / Q000502:physiology; D008279:Magnetic Resonance Imaging; D051379:Mice; D018345:Mice, Knockout; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; C121100:adenylyl cyclase 6