Transcriptional response of E. coli upon FimH-mediated fimbrial adhesion.
Functionalities which may be genetically programmed into a bacterium are limited by its range of possible activities and its sensory capabilities. Therefore, enhancing the bacterial sensory repertoire is a crucial step for expanded utility in potential biomedical, industrial or environmental applications. Using microarray and qRT-PCR analyses, we have investigated transcription in E. coli (strain CSH50) following FimH-mediated adhesion to biocompatible substrates. Specifically, wild-type FimH-mediated adhesion of E. coli to mannose agarose beads and His-tagged FimH-mediated adhesion to Ni(2+)-NTA beads both led to induction of ahpCF, dps, grxA and marRAB genes among bound cells relative to unbound cells. The strongly-induced genes are known to be regulated by OxyR or SoxS cytoplasmic redox sensors. Some differentially altered genes also overlapped with those implicated in biofilm formation. This study provides an insight into transcriptional events following FimH-mediated adhesion and may provide a platform for elucidation of the signaling circuit necessary for engineering a synthetic attachment response in E. coli.
Bhomkar
Prasanna
P
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada. bhomkar@ualberta.ca
Materi
Wayne
W
Semenchenko
Valentyna
V
Wishart
David S
DS
Science. 2009 Jan 30;323(5914):642-4
19179533
Annu Rev Microbiol. 2002;56:187-209
12142477
J Bacteriol. 1998 May;180(9):2442-9
9573197
Nature. 2000 Jan 20;403(6767):335-8
10659856
Appl Environ Microbiol. 2001 Dec;67(12):5467-73
11722894
Appl Microbiol Biotechnol. 2004 May;64(4):515-24
14727089
J Bacteriol. 1997 Dec;179(24):7724-33
9401031
Mol Microbiol. 2002 May;44(4):903-15
12010488
Science. 1999 Aug 13;285(5430):1061-6
10446051
Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2287-92
11830644
Nat Rev Microbiol. 2007 Dec;5(12):928-38
17940533
J Cell Biol. 2009 Jun 29;185(7):1275-84
19564406
Appl Environ Microbiol. 2005 Jul;71(7):4022-34
16000817
J Bacteriol. 1999 Oct;181(19):5993-6002
10498711
Environ Microbiol. 2009 Jan;11(1):1-15
19125816
Curr Opin Biotechnol. 2000 Oct;11(5):429-33
11024358
BMC Bioinformatics. 2006 Jan 06;7:5
16398937
Science. 2002 May 24;296(5572):1466-70
12029133
Science. 1996 Aug 30;273(5279):1234-6
8703059
Environ Microbiol. 2007 Feb;9(2):332-46
17222132
Microbiology (Reading). 2006 Aug;152(Pt 8):2233-2245
16849790
Infect Immun. 2007 Feb;75(2):966-76
17145952
Annu Rev Biomed Eng. 2003;5:179-206
14527313
J Bacteriol. 2007 Apr;189(8):3051-62
17293424
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D293-5
14681416
Curr Opin Microbiol. 2005 Apr;8(2):222-7
15802256
J Biol Chem. 2008 Apr 25;283(17):11596-605
18292092
Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2081-5
7892228
Infect Immun. 2005 Feb;73(2):1226-31
15664970
Methods. 2001 Dec;25(4):402-8
11846609
FEMS Microbiol Lett. 1996 Apr 1;137(2-3):257-63
8998995
Mol Microbiol. 2003 Apr;48(1):253-67
12657059
Mol Microbiol. 2004 Feb;51(3):659-74
14731270
Appl Environ Microbiol. 1998 May;64(5):1628-33
9572927
Microbiology (Reading). 1995 Nov;141 ( Pt 11):2839-48
8535512
Trends Microbiol. 2005 Jan;13(1):16-9
15639627
J Bacteriol. 2006 Jun;188(11):3731-9
16707665
Nucleic Acids Res. 2005 Jan 1;33(Database issue):D334-7
15608210
J Mol Biol. 2002 Nov 8;323(5):845-57
12417198
Biophys J. 2006 Feb 1;90(3):753-64
16272438