title: Pathogenesis of Junonia coenia densovirus in Spodoptera frugiperda: a route of infection that leads to hypoxia.
abstract: To evaluate densovirus potential against lepidopteran pests and their capacity to invade new hosts, we have characterised in vivo the infection and pathogenesis of the Junonia coenia densovirus (JcDNV) in the noctuid pest Spodoptera frugiperda. Here we show that infection starts with the ingestion of viral particles that cross the midgut epithelium without replicating. By quantitative PCR we established the kinetic and the route of infection, from virus ingestion to replication in visceral tracheae and hemocytes. JcDNV has a high particle-to-infection ratio mostly due to the barrier function of the midgut. Pathology and cytopathology suggested that infection of tracheal cells impairs oxygen delivery to demanding tissues leading to cytopathic effects in all the tissues. Finally, larval death results from several physiological shocks, including molting arrest and anoxia.
authors.affiliation: INRA, UMR 1231, F-34000 Montpellier, France.
authors.forename: Doriane
authors.initials: D
authors.lastname: Mutuel
authors.forename: Marc
authors.initials: M
authors.lastname: Ravallec
authors.forename: Beatrice
authors.initials: B
authors.lastname: Chabi
authors.forename: Cecilia
authors.initials: C
authors.lastname: Multeau
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Salmon
authors.forename: Philippe
authors.initials: P
authors.lastname: Fournier
authors.forename: Mylène
authors.initials: M
authors.lastname: Ogliastro
mesh_terms: D000818:Animals; D017990:Densovirus / Q000472:pathogenicity*; D007814:Larva / Q000821:virology; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D018411:Spodoptera / Q000821:virology*; D014132:Trachea / Q000473:pathology / Q000821:virology
chemical_list: D010100:Oxygen