Laser scanning microscopy combined with image restoration to analyse a 3D model of the human epithelial airway barrier.
A laser scanning microscope collects information from a thin, focal plane and ignores out of focus information. During the past few years it has become the standard imaging method to characterise cellular morphology and structures in static as well as in living samples. Laser scanning microscopy combined with digital image restoration is an excellent tool for analysing the cellular cytoarchitecture, expression of specific proteins and interactions of various cell types, thus defining valid criteria for the optimisation of cell culture models. We have used this tool to establish and evaluate a three dimensional model of the human epithelial airway wall.
Rothen-Rutishauser
Barbara
B
Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland. rothen@ana.unibe.ch
Lehmann
Andrea D
AD
Clift
Martin J D
MJ
Blank
Fabian
F
Gehr
Peter
P
D002450:Cell Communication / Q000502:physiology; D018920:Coculture Techniques; D003198:Computer Simulation; D003713:Dendritic Cells / Q000648:ultrastructure; D004847:Epithelial Cells / Q000648:ultrastructure; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008264:Macrophages / Q000648:ultrastructure; D018613:Microscopy, Confocal / Q000379:methods*; D020545:Respiratory Mucosa / Q000033:anatomy & histology*