Wiring of pyranose dehydrogenase with osmium polymers of different redox potentials.
In this study, five different flexible osmium based redox polymers were investigated for their ability to efficiently "wire" the oxidoreductase pyranose dehydrogenase (PDH, EC 1.1.99.29) from Agaricus meleagris, on graphite electrodes for possible applications in biofuel cells. A series of newly synthesised osmium based redox polymers covering the potential range between -270 and +160 mV vs. Ag|AgCl (0.1M KCl) was used. The performance of the redox polymers for enzyme wiring was investigated using glucose as substrate. The optimal operational conditions such as pH and potential were investigated.
Zafar
Muhammad Nadeem
MN
Department of Analytical Chemistry, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Tasca
Federico
F
Boland
Susan
S
Kujawa
Magdalena
M
Patel
Ilabahen
I
Peterbauer
Clemens K
CK
Leech
Dónal
D
Gorton
Lo
L
D000364:Agaricus / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009942:Organometallic Compounds / Q000737:chemistry*; D009992:Osmium / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*; D012834:Silver / Q000737:chemistry; D018030:Silver Compounds / Q000737:chemistry
D009942:Organometallic Compounds; D011108:Polymers; D018030:Silver Compounds; D009992:Osmium; D012834:Silver; D006108:Graphite; D002237:Carbohydrate Dehydrogenases; D005947:Glucose; C037548:silver chloride