title: Variability of two plaque sampling methods in quantitation of Streptococcus mutans.
abstract: The aim of this study was to determine the reproducibility of individual versus pooled plaque sampling of permanent first molars (PFM) to quantitate Streptococcus mutans (SM)/total streptococci (TS). Ten individual and pooled plaque samples were collected from 35 subjects, randomly assigned to individual-first or pooled-first group. Plaque samples were processed and quantified for SM and TS. SM/TS ratio was used to determine the reproducibility within two group samples. Mean percentage of SM/TS in both methods were not significantly different. However, within subject detection of SM was found to be significantly more sensitive for individual sampling method. Despite the lack of a difference between both methods for SM/TS quantitation, the difference in SM detection suggests that individual sampling is more sensitive.
authors.affiliation: University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: K-L C
authors.initials: KL
authors.lastname: Hsu
authors.forename: R C
authors.initials: RC
authors.lastname: Osgood
authors.forename: G R
authors.initials: GR
authors.lastname: Cutter
authors.forename: N K
authors.initials: NK
authors.lastname: Childers
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D015169:Colony Count, Microbial / Q000379:methods; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008963:Molar / Q000382:microbiology; D013291:Streptococcus / Q000302:isolation & purification; D013295:Streptococcus mutans / Q000302:isolation & purification*; D055815:Young Adult
references.citation: Eur J Oral Sci. 1995 Feb;103(1):36-41
references.pmid: 7600248
references.citation: Biotechniques. 1998 Jun;24(6):954-8, 960, 962
references.pmid: 9631186
references.citation: Arch Oral Biol. 1995 Apr;40(4):345-51
references.pmid: 7605262
references.citation: Vital Health Stat 11. 2007 Apr;(248):1-92
references.pmid: 17633507
references.citation: J Dent Educ. 2001 Oct;65(10):1028-37
references.pmid: 11699974
references.citation: Genome Res. 1996 Oct;6(10):986-94
references.pmid: 8908518
references.citation: Arch Oral Biol. 1997 Feb;42(2):101-11
references.pmid: 9134122
references.citation: Arch Oral Biol. 1973 Nov;18(11):1357-64
references.pmid: 4518755
references.citation: J Clin Microbiol. 1976 Jul;4(1):95-8
references.pmid: 956364
references.citation: J Clin Microbiol. 1984 Oct;20(4):653-9
references.pmid: 6490852
references.citation: Microbiol Rev. 1986 Dec;50(4):353-80
references.pmid: 3540569