title: [Separation of derivatized aliphatic aldehydes in beverage by nonaqueous capillary electrophoresis].
abstract: A simple and mild method for the separation of aliphatic aldehydes based on a condensation reaction with 9,10-phenanthrenequinone as labeling reagent with nonaqueous capillary electrophoresis has been developed. The detection was performed with a diode array detector (DAD). A 58.5 cm (50 cm effective length) x 50 microm i.d. untreated fused-silica capillary was used. To optimize the conditions, the background electrolyte concentration, column temperature, voltage and other factors were evaluated. The results indicated that the buffer concentration had a great impact on the separation, but the influences of temperature and added additives on the resolution were not obvious. The optimized conditions were as follows: 80 mmol/L ammonium acetate, 1.4 mol/L acetic acid, voltage of 28 kV, column temperature of 20 degrees C and DAD detection at 254 nm. The samples were introduced atmospherically with the injection at 5 kPa (50 mbar) for 8 s. The results indicate that seven aliphatic aldehyde derivatives and actual samples can be achieved baseline resolution under the proposed conditions.
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, College of Chemistry Science, Qufu Normal University, Qufu 273165, China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Bai
authors.forename: Yanbao
authors.initials: Y
authors.lastname: Wang
authors.forename: Huaixin
authors.initials: H
authors.lastname: Zhao
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Sun
authors.forename: Lian
authors.initials: L
authors.lastname: Xia
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Fu
authors.forename: Yourui
authors.initials: Y
authors.lastname: Suo
authors.forename: Yulin
authors.initials: Y
authors.lastname: Li
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
mesh_terms: D000447:Aldehydes / Q000302:isolation & purification*; D001628:Beverages / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*
chemical_list: D000447:Aldehydes