[Atomic force microscopy detection of serological markers of viral hepatites B and C].
The aim of the study was to demonstrate the possibility of detection of serological markers, containing the hepatitis B surface antigen (HBsAg) and hepatitis C virus core-antigen (HCVcoreAg) in human serum, by use of a new atomic force microscopy (AFM)-based nanotechnological approach. In this study, the immobilization on AFM-chip of antibodies against the hepatitis B virus surface antigen (anti-HBsAg) as well as the antibodies against the hepatitis C virus core antigen (anti-HCVcoreAg) was performed. It was shown that such approach enables to detect: HBsAg, HCVcoreAg and the viral fragments containing these antigens in the serum. Comparative analysis of detection of HBsAg- and HCVcoreAg-containing particles by use of the AFM method vs. traditional methods (ELISA, PCR) has demonstrated the 75% coincidence of results between the AFM and the latter two methods.
Ivanov
Iu D
IuD
Frantsuzov
P A
PA
Pleshakova
T O
TO
Ziborov
V S
VS
Svetlov
S K
SK
Krokhin
N V
NV
Konev
V A
VA
Kovalev
O B
OB
Uchaĭkin
V F
VF
Iastrebova
O N
ON
Sveshnikov
P G
PG
Archakov
A I
AI
D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D016174:Hepacivirus; D006509:Hepatitis B / Q000097:blood*; D006510:Hepatitis B Antibodies / Q000737:chemistry; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006515:Hepatitis B virus; D006526:Hepatitis C / Q000097:blood*; D018937:Hepatitis C Antibodies / Q000737:chemistry; D018936:Hepatitis C Antigens / Q000097:blood*; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D015415:Biomarkers; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies; D018936:Hepatitis C Antigens