Marine Bacillus spp. associated with the egg capsule of Concholepas concholepas (common name "loco") have an inhibitory activity toward the pathogen Vibrio parahaemolyticus.
The pandemic bacterium Vibrio parahaemolyticus, isolated from seawater, sediment, and marine organisms, is responsible for gastroenteric illnesses in humans and also cause diseases in aquaculture industry in Chile and other countries around the world. In this study, bacterial flora with inhibitory activity against pathogenic V. parahaemolyticus were collected from egg capsules of Concholepas concholepas and evaluated. The 16S rRNA fragment was sequenced from each isolated strain to determine its identity using the GenBank database. A phylogenetic analysis was made, and tests for the productions of antibacterial substance were performed using the double-layer method. Forty-five morphotypes of bacterial colonies were isolated, 8 of which presented an inhibitory effect on the growth of V. parahaemolyticus. 16S rRNA sequence and phylogenetic analysis show that these strains constitute taxa that are phylogenetically related to the Bacillus genus and are probably sister species or strains of the species Bacillus pumilus, Bacillus licheniform, or Bacillus sp. It is important to determine the nature of the antibacterial substance to evaluate their potential for use against the pathogen species V. parahaemolyticus.
Leyton
Yanett
Y
Microbial Ecology Laboratory, Aquaculture Department, Faculty of Marine Resources, University of Antofagasta, Casilla 170, Antofagasta, Chile. yleyton@uantof.cl
Riquelme
Carlos
C
D000818:Animals; D000898:Antibiosis; D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics; D049851:Gastropoda / Q000382:microbiology*; D010063:Ovum / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014736:Vibrio parahaemolyticus / Q000502:physiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
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