[Establishment of one-step reverse transcription-polymerase chain reaction method for mumps virus hydrophobin gene detection].
OBJECTIVE
To establish a kind of simple method for identifying mumps virus gene: one-step reverse transcription-polymerase chain reaction, RT-PCR).


METHODS
To design primer in both sides of SH gene, and then to do the sensitivity and specificity test for the RT-PCR method.


RESULTS
At least 10(0.7) TCID50 mumps virus can be detected by this method. 5 strains of mumps virus can be detected and 4 strains of non-mumps virus including measles and rubella virus can't be detected by this method.


CONCLUSIONS
The one-step RT-PCR method is a rapid and simple method for identifying mumps virus gene.
Chang
Xin
X
Jilin Provincial Center for Disease Control and Prevention, Key Laboratory of Health Bureau of Jilin Province, Changchun 130062, Jilin, China.
Zhou
Jian-Hui
JH
Wang
Shuang
S
D005814:Genes, Viral / Q000235:genetics*; D006801:Humans; D009109:Mumps virus / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors