title: Use of a single-chain antibody library for ovarian cancer biomarker discovery.
abstract: The discovery of novel early detection biomarkers of disease could offer one of the best approaches to decrease the morbidity and mortality of ovarian and other cancers. We report on the use of a single-chain variable fragment antibody library for screening ovarian serum to find novel biomarkers for the detection of cancer. We alternately panned the library with ovarian cancer and disease-free control sera to make a sublibrary of antibodies that bind proteins differentially expressed in cancer. This sublibrary was printed on antibody microarrays that were incubated with labeled serum from multiple sets of cancer patients and controls. The antibodies that performed best at discriminating disease status were selected, and their cognate antigens were identified using a functional protein microarray. Overexpression of some of these antigens was observed in cancer serum, tumor proximal fluid, and cancer tissue via dot blot and immunohistochemical staining. Thus, our use of recombinant antibody microarrays for unbiased discovery found targets for ovarian cancer detection in multiple sample sets, supporting their further study for disease diagnosis.
authors.affiliation: Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
authors.forename: Arturo B
authors.initials: AB
authors.lastname: Ramirez
authors.forename: Christian M
authors.initials: CM
authors.lastname: Loch
authors.forename: Yuzheng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Wayner
authors.forename: Jonathon E
authors.initials: JE
authors.lastname: Sargent
authors.forename: Sahar
authors.initials: S
authors.lastname: Sibani
authors.forename: Eugenie
authors.initials: E
authors.lastname: Hainsworth
authors.forename: Eliseo A
authors.initials: EA
authors.lastname: Mendoza
authors.forename: Ralph
authors.initials: R
authors.lastname: Eugene
authors.forename: Joshua
authors.initials: J
authors.lastname: Labaer
authors.forename: Nicole D
authors.initials: ND
authors.lastname: Urban
authors.forename: Martin W
authors.initials: MW
authors.lastname: McIntosh
authors.forename: Paul D
authors.initials: PD
authors.lastname: Lampe
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000097:blood / Q000276:immunology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D015723:Gene Library; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D012307:Risk Factors; D057127:Single-Chain Antibodies / Q000276:immunology; D055815:Young Adult
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D057127:Single-Chain Antibodies
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