title: Single point vs. mapping approach for spectral cytopathology (SCP).
abstract: In this paper we describe the advantages of collecting infrared microspectral data in imaging mode opposed to point mode. Imaging data are processed using the PapMap algorithm, which co-adds pixel spectra that have been scrutinized for R-Mie scattering effects as well as other constraints. The signal-to-noise quality of PapMap spectra will be compared to point spectra for oral mucosa cells deposited onto low-e slides. Also the effects of software atmospheric correction will be discussed. Combined with the PapMap algorithm, data collection in imaging mode proves to be a superior method for spectral cytopathology.
authors.affiliation: Northeastern University, Department of Chemistry and Chemical Biology, Boston, MA 02115, USA.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Schubert
authors.forename: Antonella I
authors.initials: AI
authors.lastname: Mazur
authors.forename: Benjamin
authors.initials: B
authors.lastname: Bird
authors.forename: Milos
authors.initials: M
authors.lastname: Miljković
authors.forename: Max
authors.initials: M
authors.lastname: Diem
mesh_terms: D000818:Animals; D002478:Cells, Cultured / Q000737:chemistry* / Q000145:classification*; D006801:Humans; D008853:Microscopy / Q000379:methods*; D013055:Spectrophotometry, Infrared / Q000379:methods*
references.citation: Vib Spectrosc. 2006 Oct 18;42(1):9-14
references.pmid: 19750140
references.citation: Arch Pathol Lab Med. 1997 Mar;121(3):211-24
references.pmid: 9111103
references.citation: Biochim Biophys Acta. 2006 Jul;1758(7):900-7
references.pmid: 16815240
references.citation: Biochim Biophys Acta. 2006 Jul;1758(7):892-9
references.pmid: 16820140
references.citation: Biochim Biophys Acta. 2006 Jul;1758(7):915-22
references.pmid: 16797481
references.citation: JAMA. 2001 Mar 21;285(11):1500-5
references.pmid: 11255427
references.citation: Lab Invest. 2010 Jul;90(7):1068-77
references.pmid: 20368702
references.citation: Cytopathology. 2005 Feb;16(1):7-12
references.pmid: 15859309
references.citation: Annu Rev Phys Chem. 2005;56:429-74
references.pmid: 15796707
references.citation: J Biophotonics. 2010 Aug;3(8-9):597-608
references.pmid: 20437419
references.citation: Analyst. 2004 Oct;129(10):880-5
references.pmid: 15457314
references.citation: Analyst. 2009 Aug;134(8):1586-93
references.pmid: 20448924
references.citation: Analyst. 2009 Jun;134(6):1171-5
references.pmid: 19475144
references.citation: Lab Invest. 2010 Apr;90(4):589-98
references.pmid: 20142808
references.citation: Analyst. 2010 Feb;135(2):268-77
references.pmid: 20098758