title: The identification, expression profile, and preliminary characterization of Tsunagi protein from Schistosoma japonicum.
abstract: Tsunagi is an evolutionarily conserved protein, which is required for germ line differentiation during the development of Drosophila melanogaster and Caenorhabditis elegans. In this paper, we describe a homologue of the Tsunagi protein from Schistosoma japonicum (SjTsunagi). The gene for this protein was isolated from S. japonicum using degenerate and anchored polymerase chain reaction (PCR), and the deduced protein has sequence homology and similarity to Tsunagi protein of other species, including C. elegans, D. melanogaster, Xenopus laevis, Mus musculus, and human. Amino acid sequence analysis showed the presence of a conserved RNA recognition motif. The predicted protein encoded by SjTsunagi gene is 177 amino acids in length with an estimated molecular mass of 20 kD. Immunoblot and reverse transcription-PCR analysis confirms SjTsunagi protein is expressed in eggs, cercariae, schistosomula, and adult female and adult male parasites. Pull-down and co-immunoprecipitation assay confirms that protein of SjTsunagi can interact with SjMago nashi in vitro. Taken together, this is the first report of the expression and preliminary characterization analysis of the SjTsunagi gene from S. japonicum.
authors.affiliation: Department of Parasitology, Anhui Medical University, Hefei, Anhui, People's Republic of China.
authors.forename: Miao
authors.initials: M
authors.lastname: Liu
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Lei
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Zhao
authors.forename: Jijia
authors.initials: J
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D029721:Drosophila Proteins / Q000737:chemistry; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015151:Immunoblotting; D008297:Male; D008969:Molecular Sequence Data; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D029721:Drosophila Proteins; D015801:Helminth Proteins; D016601:RNA-Binding Proteins; C440016:tsu protein, Drosophila
references.citation: Nucleic Acids Res. 1998 Apr 1;26(7):1628-35
references.pmid: 9512532
references.citation: Genomics. 2000 Jan 1;63(1):145-8
references.pmid: 10662555
references.citation: Biochem Biophys Res Commun. 2009 Apr 24;382(1):63-8
references.pmid: 19254694
references.citation: Cell. 1999 Feb 5;96(3):307-10
references.pmid: 10025395
references.citation: Exp Parasitol. 2008 Jul;119(3):379-84
references.pmid: 18466902
references.citation: Mech Dev. 2004 Jan;121(1):27-35
references.pmid: 14706697
references.citation: Dev Biol. 2000 Feb 15;218(2):172-82
references.pmid: 10656761
references.citation: Dev Biol. 2007 Aug 15;308(2):507-19
references.pmid: 17628520
references.citation: EMBO J. 2000 Dec 15;19(24):6860-9
references.pmid: 11118221
references.citation: Development. 1994 May;120(5):1303-13
references.pmid: 8026338
references.citation: Development. 1991 Sep;113(1):373-84
references.pmid: 1765008
references.citation: EMBO J. 2001 Apr 17;20(8):2062-8
references.pmid: 11296238
references.citation: J Cell Sci. 2002 Aug 1;115(Pt 15):3033-8
references.pmid: 12118059
references.citation: Nature. 2004 Apr 29;428(6986):959-63
references.pmid: 15118729
references.citation: EMBO J. 2001 Nov 15;20(22):6424-33
references.pmid: 11707413
references.citation: Curr Biol. 1997 Jul 1;7(7):468-78
references.pmid: 9210377
references.citation: Development. 1997 Aug;124(16):3197-207
references.pmid: 9272960
references.citation: Nat Genet. 2003 Oct;35(2):148-57
references.pmid: 12973350
references.citation: Curr Opin Struct Biol. 2002 Jun;12(3):283-8
references.pmid: 12127445
references.citation: Science. 1994 Jul 29;265(5172):615-21
references.pmid: 8036511
references.citation: Mol Cell. 2000 Sep;6(3):673-82
references.pmid: 11030346
references.citation: Genes Dev. 2001 Nov 1;15(21):2886-99
references.pmid: 11691839
references.citation: Genomics. 1998 Jan 15;47(2):319-22
references.pmid: 9479507
references.citation: Curr Biol. 2001 Oct 30;11(21):1666-74
references.pmid: 11696323
references.citation: Nat Struct Biol. 2003 Jun;10(6):433-9
references.pmid: 12730685