title: Nucleosome interaction surface of linker histone H1c is distinct from that of H1(0).
abstract: The fully organized structure of the eukaryotic nucleosome remains unsolved, in part due to limited information regarding the binding site of the H1 or linker histone. The central globular domain of H1 is believed to interact with the nucleosome core at or near the dyad and to bind at least two strands of DNA. We utilized site-directed mutagenesis and in vivo photobleaching to identify residues that contribute to the binding of the globular domain of the somatic H1 subtype H1c to the nucleosome. As was previously observed for the H1(0) subtype, the binding residues for H1c are clustered on the surface of one face of the domain. Despite considerable structural conservation between the globular domains of these two subtypes, the locations of the binding sites identified for H1c are distinct from those of H1(0). We suggest that the globular domains of these two linker histone subtypes will bind to the nucleosome with distinct orientations that may contribute to higher order chromatin structure heterogeneity or to differences in dynamic interactions with other DNA or chromatin-binding proteins.
authors.affiliation: Department of Biochemistry, University of Mississippi Medical Center, Jackson, MI 39216-4505, USA.
authors.forename: Eric M
authors.initials: EM
authors.lastname: George
authors.forename: Tina
authors.initials: T
authors.lastname: Izard
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Anderson
authors.forename: David T
authors.initials: DT
authors.lastname: Brown
mesh_terms: D001665:Binding Sites; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D008968:Molecular Conformation; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed; D009707:Nucleosomes / Q000378:metabolism*
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D049452:Green Fluorescent Proteins; D004247:DNA
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