Dual reporter system for in situ detection of plasmid transfer under aerobic and anaerobic conditions.
We designed a new genetic tool to detect plasmid transfer under anaerobic and aerobic conditions. The system is based on the T7 RNA polymerase gene and a T7 promoter-driven oxygen-independent green fluorescent protein, evoglow, alone or in combination with red fluorescent protein DsRed. Constructs are available as plasmids and mini-mariner transposons.
Król
Jaroslaw E
JE
Department of Biological Sciences, Initiative for Bioinformatics and Evolutionary Studies, University of Idaho, P.O. Box 443051, Moscow, ID 83844-3051, USA.
Rogers
Linda M
LM
Krone
Stephen M
SM
Top
Eva M
EM
D000332:Aerobiosis; D000693:Anaerobiosis; D017123:Bacteriophage T7; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D012321:DNA-Directed RNA Polymerases; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development* / Q000821:virology; D005821:Genetic Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014764:Viral Proteins; D000097573:Red Fluorescent Protein
D004251:DNA Transposable Elements; D012321:DNA-Directed RNA Polymerases; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D014764:Viral Proteins; D000097573:Red Fluorescent Protein; C062210:bacteriophage T7 RNA polymerase
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