title: Sensitive label-free immunoassay of carcinoembryonic antigen based on Au-TiO2 hybrid nanocomposite film.
abstract: In this paper, a new nanostructured Au-TiO(2) particle was synthesised and employed for the construction of a label-free amperometric immunosensor for carcinoembryonic antigen (CEA) determination. The spherical Au-TiO(2) nanoparticles provided a good microenvironment for the immobilization of biomolecules, enhanced the surface coverage of protein, had large surface-to-volume ratio, film-forming ability and high stability. The Au-TiO(2) nanoparticles were characterized by transmission electron microscopy (TEM). The fabrication process of the electrochemical immunosensor was monitored by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. Under optimal conditions, the proposed immunosensor exhibits a good linearity at the studied concentration range from 0.2 to 160.0 ng mL(-1) towards CEA with a coefficient of 0.9965 and a detection limit of 0.06 ng mL(-1) (S/N=3). Moreover, the proposed immunosensor shows good selectivity, reproducibility, high sensitivity, and would have a potential application in clinical immunoassays.
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Yuyong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Qian
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
mesh_terms: D002272:Carcinoembryonic Antigen / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods / Q000592:standards; D006046:Gold; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D053761:Nanocomposites / Q000737:chemistry*; D015203:Reproducibility of Results; D014025:Titanium
chemical_list: D002272:Carcinoembryonic Antigen; C009495:titanium dioxide; D006046:Gold; D014025:Titanium