Preliminary X-ray crystallographic analysis of SMU.2055 protein from the caries pathogen Streptococcus mutans.
The SMU.2055 gene from the major caries pathogen Streptococcus mutans is annotated as a putative acetyltransferase with 163 amino-acid residues. In order to identify its function via structural studies, the SMU.2055 gene was cloned into the expression vector pET28a. Native and SeMet-labelled SMU.2055 proteins with a His(6) tag at the N-terminus were expressed at a high level in Escherichia coli strain BL21 (DE3) and purified to homogeneity by Ni(2+)-chelating affinity chromatography. Diffraction-quality crystals of SeMet-labelled SMU.2055 were obtained using the sitting-drop vapour-diffusion method and diffracted to a resolution of 2.5 A on beamline BL17A at the Photon Factory, Tsukuba, Japan. The crystals belong to the orthorhombic space group C222(1), with unit-cell parameters a = 92.0, b = 95.0, c = 192.2 A. The asymmetric unit contained four molecules, with a solvent content of 57.1%.
Zhao
Wang Hong
WH
School of Stomatology, Peking University, Beijing 100081, People's Republic of China.
Zhan
Xiu Rong
XR
Gao
Xiong Zhuo
XZ
Liu
Xiang
X
Zhang
Yi Fei
YF
Lin
Jiuxiang
J
Li
Lan Fen
LF
Wei
Shi Cheng
SC
Su
Xio Dong
XD
D000123:Acetyltransferases / Q000737:chemistry*; D018360:Crystallography, X-Ray; D013295:Streptococcus mutans / Q000201:enzymology*
D000123:Acetyltransferases
Methods Enzymol. 1997;276:523-30
9048379
Vet Clin North Am Equine Pract. 1998 Aug;14(2):349-63, vii
9742668
Science. 1988 Jan 29;239(4839):487-91
2448875
Med Sci Monit. 2007 Nov;13(11):RA196-203
17968308
Heart Lung. 1988 Mar;17(2):209-12
3350687
Crit Rev Oral Biol Med. 2001;12(4):301-14
11603503
Acta Crystallogr D Biol Crystallogr. 2006 Aug;62(Pt 8):843-51
16855299
Adv Exp Med Biol. 1992;327:229-41
1295342
Arch Biochem Biophys. 2005 Jan 1;433(1):212-26
15581578
Indian J Dent Res. 2009 Jan-Mar;20(1):99-106
19336869
Am Fam Physician. 2008 Mar 15;77(6):797-802
18386594
Microbiol Rev. 1986 Dec;50(4):353-80
3540569
Am J Dent. 2009 Feb;22(1):3-8
19281105
J Mol Biol. 1968 Apr 28;33(2):491-7
5700707