Anatomically shaped tooth and periodontal regeneration by cell homing.
Tooth regeneration by cell delivery encounters translational hurdles. We hypothesized that anatomically correct teeth can regenerate in scaffolds without cell transplantation. Novel, anatomically shaped human molar scaffolds and rat incisor scaffolds were fabricated by 3D bioprinting from a hybrid of poly-epsilon-caprolactone and hydroxyapatite with 200-microm-diameter interconnecting microchannels. In each of 22 rats, an incisor scaffold was implanted orthotopically following mandibular incisor extraction, whereas a human molar scaffold was implanted ectopically into the dorsum. Stromal-derived factor-1 (SDF1) and bone morphogenetic protein-7 (BMP7) were delivered in scaffold microchannels. After 9 weeks, a putative periodontal ligament and new bone regenerated at the interface of rat incisor scaffold with native alveolar bone. SDF1 and BMP7 delivery not only recruited significantly more endogenous cells, but also elaborated greater angiogenesis than growth-factor-free control scaffolds. Regeneration of tooth-like structures and periodontal integration by cell homing provide an alternative to cell delivery, and may accelerate clinical applications.
Kim
K
K
Columbia University College of Dental Medicine, 630 W. 168th St., PH7E - CDM, New York, NY 10032, USA.
Lee
C H
CH
Kim
B K
BK
Mao
J J
JJ
D000539:Alveolar Process / Q000502:physiology; D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000008:administration & dosage / Q000502:physiology*; D001861:Bone Regeneration; D054377:Chemokine CXCL12 / Q000008:administration & dosage / Q000502:physiology*; D017886:Durapatite; D006801:Humans; D018919:Neovascularization, Physiologic; D010513:Periodontal Ligament / Q000502:physiology; D011091:Polyesters; D051381:Rats; D012038:Regeneration; D055153:Stem Cell Niche / Q000502:physiology*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014070:Tooth / Q000502:physiology*
D055419:Bone Morphogenetic Protein 7; D054377:Chemokine CXCL12; D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite
J Oral Maxillofac Surg. 2009 Feb;67(2):335-47
19138608
J Dent Res. 2008 Dec;87(12):1138-43
19029082
Genomics. 1992 Nov;14(3):759-62
1427904
Ann N Y Acad Sci. 2009 Sep;1176:118-23
19796239
Cell Stem Cell. 2008 Jun 5;2(6):566-75
18522849
J Oral Rehabil. 2007 Dec;34(12):933-9
18034675
J Dent Res. 2004 Jul;83(7):518-22
15218039
Lancet. 2005 Nov 19;366(9499):1809-20
16298220
Blood. 2003 Apr 1;101(7):2609-16
12468435
Stem Cells Dev. 2004 Aug;13(4):436-48
15345137
Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13475-80
19666587
Tissue Eng Part A. 2008 Nov;14(11):1763-73
18636943
EMBO J. 2001 Aug 1;20(15):4132-42
11483516
Expert Opin Biol Ther. 2009 Sep;9(9):1143-54
19653863
J Dent Res. 2004 Jul;83(7):523-8
15218040
PLoS One. 2010 Oct 20;5(10):e13547
20975999
Arch Oral Biol. 2005 Feb;50(2):255-8
15721158
Tissue Eng. 2005 Sep-Oct;11(9-10):1599-610
16259613
Nat Methods. 2007 Mar;4(3):227-30
17322892
J Dent Res. 2006 Nov;85(11):966-79
17062735
Tissue Eng Part A. 2009 Dec;15(12):3923-30
19563263
Tissue Eng. 2005 Sep-Oct;11(9-10):1297-311
16259586
Methods. 2009 Feb;47(2):116-21
18952179
Tissue Eng Part A. 2008 Apr;14(4):549-57
18352829
J Dent Res. 2008 Aug;87(8):745-50
18650546
J Dent Res. 2002 Oct;81(10):695-700
12351668
Arthritis Rheum. 2009 Mar;60(3):813-23
19248097
Adv Dent Res. 2001 Aug;15:91-5
12640750
J Biomed Mater Res A. 2008 Sep 15;86(4):1062-8
18067171
PLoS One. 2006 Dec 20;1:e79
17183711
Dent Clin North Am. 2006 Apr;50(2):191-203, viii
16530057
Eur J Oral Sci. 2001 Dec;109(6):422-4
11767280
Tissue Eng Part A. 2008 Dec;14(12):2089-104
19093294
Tissue Eng Part B Rev. 2010 Apr;16(2):257-62
19891541