Improvement of renal oxidative stress markers after ozone administration in diabetic nephropathy in rats.
BACKGROUND
Several complications of diabetes mellitus (DM) e.g. nephropathy (DN) have been linked to oxidative stress. Ozone, by means of oxidative preconditioning, may exert its protective effects on DN.


OBJECTIVE
The aim of the present work is to study the possible role of ozone therapy in ameliorating oxidative stress and inducing renal antioxidant defence in streptozotocin (STZ)-induced diabetic rats.


METHODS
Six groups (n = 10) of male Sprague Dawley rats were used as follows: Group C: Control group. Group O: Ozone group, in which animals received ozone intraperitoneally (i.p.) (1.1 mg/kg). Group D: Diabetic group, in which DM was induced by single i.p. injections of streptozotocin (STZ). Group DI: Similar to group D but animals also received subcutaneous (SC) insulin (0.75 IU/100 gm BW.). Group DO: In which diabetic rats received the same dose of ozone, 48 h after induction of diabetes. Group DIO, in which diabetic rats received the same doses of insulin and ozone, respectively. All animals received daily treatment for six weeks. At the end of the study period (6 weeks), blood pressure, blood glycosylated hemoglobin (HbA1c), serum creatinine, blood urea nitrogen (BUN), kidney tissue levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxide (GPx), aldose reductase (AR) activities and malondialdehyde (MDA) concentration were measured.


RESULTS
Induction of DM in rats significantly elevated blood pressure, HbA1c, BUN, creatinine and renal tissue levels of MDA and AR while significantly reducing SOD, CAT and GPx activities. Either Insulin or ozone therapy significantly reversed the effects of DM on all parameters; in combination (DIO group), they caused significant improvements in all parameters in comparison to each alone.


CONCLUSIONS
Ozone administration in conjunction with insulin in DM rats reduces oxidative stress markers and improves renal antioxidant enzyme activity which highlights its potential uses in the regimen for treatment of diabetic patients.
Morsy
Mohamed D
MD
Department of Physiology, College of Medicine, Menoufiya University, Egypt. morsydarwesh@yahoo.com.
Hassan
Waleed N
WN
Zalat
Sherif I
SI
Clin Chem Lab Med. 1998 Oct;36(10):771-5
9853804
Free Radic Biol Med. 2002 Mar 15;32(6):551-61
11958956
Pak J Pharm Sci. 2009 Apr;22(2):205-10
19339234
Iran J Kidney Dis. 2009 Jan;3(1):12-6
19377253
Pharmacol Res. 2001 Nov;44(5):391-6
11712870
ScientificWorldJournal. 2008 Aug 31;8:835-44
18758661
Free Radic Res. 2000 Nov;33(5):595-605
11200091
Comp Biochem Physiol C Toxicol Pharmacol. 2003 Nov;136(3):205-12
14659454
Diabetes. 2003 Jan;52(1):93-101
12502498
Eur J Pharmacol. 2005 Oct 31;523(1-3):151-61
16198334
Diabetes. 2006 Jun;55(6):1651-9
16731827
Diabetes Res Clin Pract. 2008 Nov 13;82 Suppl 1:S25-9
18954918
Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10587-92
12149437
Nature. 2000 Apr 13;404(6779):787-90
10783895
Res Commun Chem Pathol Pharmacol. 1991 May;72(2):191-202
1876750
Diabetes Metab Res Rev. 2001 May-Jun;17(3):223-30
11424235
Arch Biochem Biophys. 1972 Jun;150(2):606-17
5044042
Singapore Med J. 2006 Aug;47(8):670-5
16865205
J Lab Clin Med. 1967 Jul;70(1):158-69
6066618
Tree Physiol. 2008 Dec;28(12):1761-72
19193559
Diabete Metab. 1995 Oct;21(4):252-5
8529759
Mediators Inflamm. 1999;8(1):37-41
10704088
Nephron Exp Nephrol. 2009;111(1):e20-30
19052474
Curr Vasc Pharmacol. 2006 Jul;4(3):215-27
16842139
Eur J Pharmacol. 2009 Jun 10;612(1-3):106-14
19356735
Physiol Genomics. 2005 Apr 14;21(2):222-9
15713786
J Clin Invest. 1995 Apr;95(4):1710-6
7535795
J Appl Toxicol. 2001 Jul-Aug;21(4):297-301
11481663
Biochem Biophys Res Commun. 1972 Jan 31;46(2):849-54
4400444
Clin Chem. 1976 Oct;22(10):1614-7
975505
Free Radic Biol Med. 1995 Jul;19(1):115-9
7635353
J Surg Res. 2008 Oct;149(2):287-95
18262565
Anal Biochem. 1979 Jun;95(2):351-8
36810
Endocrine. 2009 Aug;36(1):45-51
19390997
Clin Chim Acta. 1981 Feb 5;109(3):267-74
7226519
Biochim Biophys Acta. 1991 Jun 24;1078(2):171-8
1905957
Pharmacol Res. 2002 Mar;45(3):249-52
11884223
J Clin Pathol. 1969 Mar;22(2):246
5776563
J Am Coll Nutr. 1998 Feb;17(1):7-10
9477383
Exp Clin Endocrinol Diabetes. 2007 Feb;115(2):69-84
17318765
Diabetes. 2002 Feb;51(2):506-13
11812762
Ann Clin Biochem. 1986 Jan;23 ( Pt 1):1-25
3532908
J Nephrol. 2009 Mar-Apr;22(2):249-54
19384843
Nephrology (Carlton). 2005 Oct;10 Suppl:S7-10
16174288
Indian J Biochem Biophys. 1984 Apr;21(2):130-2
6490072
Circulation. 1999 Aug 24;100(8):820-5
10458717
Inflamm Res. 2009 Jul;58(7):371-5
19274439