title: Structural markers in prostate cancer serum imaged ex vivo using cryogenic transmission electron microscopy.
abstract: We used cryogenic transmission electron microscopy (cryo-TEM) to identify differences in macromolecular structures present in the serum from healthy individuals (HI) and prostate cancer (PCa) patients and show that these differences are potential markers for disease. Using a murine orthotopic model of human PCa, we determined that some of these structural markers in serum are associated with the tumour burden. These findings signify the potential of this nanoscale ex vivo imaging technology of body fluids as a platform for discovering early markers of PCa and other diseases.
authors.forename: Nazneen
authors.initials: N
authors.lastname: Aziz
authors.forename: Ashish K
authors.initials: AK
authors.lastname: Jha
authors.forename: Chris
authors.initials: C
authors.lastname: Thanos
authors.forename: Riyaz
authors.initials: R
authors.lastname: Basha
authors.forename: Arijit
authors.initials: A
authors.lastname: Bose
mesh_terms: D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D020285:Cryoelectron Microscopy / Q000379:methods*; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D051379:Mice; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D008875:Middle Aged; D049329:Nanostructures / Q000648:ultrastructure*; D011471:Prostatic Neoplasms / Q000097:blood* / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor