[Effects of jingqianshu granule on expression of estrogen receptor alpha and beta mRNA in hypothalamus and hippocampus of PMS rats with liver-qi depression].
OBJECTIVE
To investigate the effects of Jingqianshu granule (JQS), a Chinese medicinal formula on the expression levels of ERa and ERbeta mRNA in hypothalamus and hippocampus of PMS model rats with liver-qi depression.


METHODS
The female SD rats were divided randomly into three groups: control, model and model plus Jingqianshu granule. We make the model rats of PMS with liver-qi depression using the method of emotional stimulation multiple factors. Then we applied semi-quantitative RT-PCR gene amplification technology to detect the expression levels of ERalpha and ERbeta mRNA in the brain regions of all rats.


RESULTS
Compared with the control group, the expression levels of ERalpha and ERbeta mRNA in hypothalamus and hippocampus of model rats were higher significantly (P < 0.05), while in the JQS-administration group, the expression levels of ERalpha and ERbeta mRNA were lower than model group (P < 0.05), and were no difference with the control group.


CONCLUSIONS
The JQS granule can down-regulate the expression levels of ERalpha and ERbeta mRNA in hypothalamus and hippocampus, which maybe one of the mechanism to treat PMS with Liver-qi depression.
Zhang
Huiyun
H
Shandong University of Traditional Chinese Medicine, Jinan 250014, China. zhhuiyun@163.com
Ma
Jing
J
D000818:Animals; D004195:Disease Models, Animal; D004304:Dosage Forms; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000502:physiology*; D011293:Premenstrual Syndrome / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D019413:Qi; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism
D004304:Dosage Forms; D004365:Drugs, Chinese Herbal; D011960:Receptors, Estrogen