title: How to track cellular aging of mesenchymal stromal cells?
abstract: Mesenchymal stromal cells (MSC) are currently tested in a large number of clinical trials and raise high hope in regenerative medicine. These cells have to be expanded in vitro before transplantation and several studies demonstrated that long-term culture evokes continuous changes in MSC: proliferation rate decays, the cell size increases, differentiation potential is affected, chromosomal instabilities may arise and molecular changes are acquired. Long-term culture of cell preparations might also have therapeutic consequences, although this has hardly been addressed in ongoing trials so far. Reliable therapeutic regimens necessitate quality control of cellular products. This research perspective summarizes available methods to track cellular aging of MSC. We have demonstrated that gene expression changes and epigenetic modifications are continuously acquired during replicative senescence. Molecular analysis of a suitable panel of genes might provide a robust tool to assess efficiency and safety of long-term expansion.
authors.affiliation: Helmholtz Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, 52074 Aachen, Germany. wwagner@ukaachen.de
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wagner
authors.forename: Simone
authors.initials: S
authors.lastname: Bork
authors.forename: Günther
authors.initials: G
authors.lastname: Lepperdinger
authors.forename: Sylvia
authors.initials: S
authors.lastname: Joussen
authors.forename: Nan
authors.initials: N
authors.lastname: Ma
authors.forename: Dirk
authors.initials: D
authors.lastname: Strunk
authors.forename: Carmen
authors.initials: C
authors.lastname: Koch
mesh_terms: D016922:Cellular Senescence; D002869:Chromosome Aberrations; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*; D016615:Telomere
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