Temperature and nutrient induced responses of Lake Fryxell sulfate-reducing prokaryotes and description of Desulfovibrio lacusfryxellense, sp. nov., a pervasive, cold-active, sulfate-reducing bacterium from Lake Fryxell, Antarctica.
The effects of temperature and carbon substrate availability on the stimulation of sulfate reduction by indigenous populations of sulfate-reducing prokaryotes (SRP) in permanently ice-covered Lake Fryxell, Antarctica were investigated. Psychrophilic and halotolerant, lactate-degrading SRP showed significant metabolic activity throughout all sampled depths of the water column, suggesting that such organisms, possibly of marine origin, may be key contributors to carbon and sulfur cycling in Lake Fryxell. Planktonic and benthic strains of lactate-oxidizing sulfate-reducing bacteria (SRB) were isolated from samples of various depths of the anoxic water column and from surficial sediments. Phylogenetic analyses of 16S rRNA gene sequences placed the Fryxell sulfate-reducer (FSR) strains within the Deltaproteobacteria and showed them to be most closely related to the Arctic marine species of SRB Desulfovibrio frigidus and Desulfovibrio ferrireducens. Based on phylogenetic and phenotypic differences between the Antarctic FSR strains and related species of the genus Desulfovibrio, strain FSRs(T) (=DSM 23315(T) =ATCC BAA-2083(T)) is proposed as the type strain of a novel species of cold-active SRB, Desulfovibrio lacusfryxellense, sp. nov.
Sattley
W Matthew
WM
Department of Microbiology, Southern Illinois University, Carbondale, IL, 62901, USA. Matthew.Sattley@indwes.edu
Madigan
Michael T
MT
D000864:Antarctic Regions; D002244:Carbon / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D003901:Desulfovibrio / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D013431:Sulfates / Q000378:metabolism*; D013696:Temperature
D004269:DNA, Bacterial; D013431:Sulfates; D002244:Carbon
Int J Syst Bacteriol. 1999 Oct;49 Pt 4:1631-43
10555345
Appl Environ Microbiol. 2006 Aug;72(8):5562-8
16885310
Int J Syst Bacteriol. 1997 Oct;47(4):1134-9
9336920
FEMS Microbiol Lett. 2007 Jul;272(1):48-54
17456187
Appl Environ Microbiol. 1999 Sep;65(9):3976-81
10473404
FEMS Microbiol Lett. 2008 Oct;287(1):121-7
18673392
Appl Environ Microbiol. 2006 Feb;72(2):1663-6
16461723
Nucleic Acids Res. 2009 Jan;37(Database issue):D141-5
19004872
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712
Appl Environ Microbiol. 2003 Aug;69(8):4910-4
12902286
Int J Syst Evol Microbiol. 2006 Apr;56(Pt 4):681-685
16585676
Antonie Van Leeuwenhoek. 2006 Jan;89(1):109-24
16328859
Antonie Van Leeuwenhoek. 1964;30:225-38
14218435
Environ Microbiol. 2000 Apr;2(2):227-37
11220308
Int J Syst Evol Microbiol. 2006 Apr;56(Pt 4):687-690
16585677
Environ Toxicol. 2002 Feb;17(1):40-8
11847973
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):173-178
12656169
Appl Environ Microbiol. 2005 Oct;71(10):6353-9
16204557
Genome Res. 1999 Sep;9(9):868-77
10508846
Arch Microbiol. 2000 Apr;173(4):269-77
10816045
Int J Syst Bacteriol. 1999 Apr;49 Pt 2:859-65
10319511
Appl Environ Microbiol. 2003 Jun;69(6):3181-91
12788715