title: Development of an in vitro model of feline cartilage degradation.
abstract: Osteoarthritis is the most common arthropathy of mammalian species including cats. Cartilage degradation is central to the disorder and here we present, for the first time, an in vitro model of feline cartilage degradation which will be useful for further studies in this target species. Feline articular cartilage explant cultures were maintained for 28 days and in the presence of oncostatin M with and without interleukin (IL)-17, tumour necrosis factor (TNF), IL-1alpha, or IL-1beta. Media samples and digested cartilage explants were analysed for glycosaminoglycan (GAG) and collagen content. The combination of IL-1beta and OSM, both at 20 ng/ml, was able to promote GAG release to the greatest extent at 14 days. At 28 days, all groups showed relatively high release of GAG. At 14 days, only IL-1beta and OSM in combination were associated with a statistically significant increase in collagen release over and above control tissue. IL-1beta dose-response studies showed that an IL-1beta dose of 10 ng/ml promotes a statistically significant increase in GAG breakdown when used with OSM, and higher doses of IL-1beta did not result in significantly greater response. The model demonstrated both GAG and collagen degradation and will be of use for further understanding of feline cartilage metabolism and for screening of potential structure-modifying agents to be used in cats.
authors.affiliation: Musculoskeletal Research Group, School of Veterinary Science, University of Liverpool, Leahurst Campus, Neston, Wirral CH64 7TE, UK.
authors.forename: Natalie
authors.initials: N
authors.lastname: Gabriel
authors.forename: John F
authors.initials: JF
authors.lastname: Innes
authors.forename: Bruce
authors.initials: B
authors.lastname: Caterson
authors.forename: Anne
authors.initials: A
authors.lastname: Vaughan-Thomas
mesh_terms: D000818:Animals; D002357:Cartilage Diseases / Q000378:metabolism / Q000662:veterinary*; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism; D002371:Cat Diseases / Q000378:metabolism*; D002415:Cats; D003094:Collagen / Q000187:drug effects / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D053583:Interleukin-1beta / Q000494:pharmacology; D008954:Models, Biological; D053683:Oncostatin M / Q000494:pharmacology; D010003:Osteoarthritis / Q000662:veterinary; D046509:Tissue Culture Techniques / Q000662:veterinary*
chemical_list: D006025:Glycosaminoglycans; D053583:Interleukin-1beta; D053683:Oncostatin M; D003094:Collagen
references.citation: J Biomech. 1998 Jun;31(6):511-7
references.pmid: 9755035
references.citation: Cell Signal. 2004 Apr;16(4):469-76
references.pmid: 14709335
references.citation: J Pharm Pharmacol. 1997 Oct;49(10):991-8
references.pmid: 9364409
references.citation: J Vet Med Sci. 2006 Nov;68(11):1239-42
references.pmid: 17146189
references.citation: Arthritis Rheum. 2002 Apr;46(4):961-7
references.pmid: 11953973
references.citation: Biochem J. 1999 Nov 15;344 Pt 1:61-8
references.pmid: 10548534
references.citation: Am J Vet Res. 2005 Jul;66(7):1278-84
references.pmid: 16111170
references.citation: Nature. 2005 Mar 31;434(7033):644-8
references.pmid: 15800624
references.citation: J Am Vet Med Assoc. 2002 Mar 1;220(5):628-32
references.pmid: 12418522
references.citation: Am J Vet Res. 1999 Dec;60(12):1546-51
references.pmid: 10622166
references.citation: Am J Vet Res. 1994 Jan;55(1):138-46
references.pmid: 8141486
references.citation: Bioessays. 1995 Dec;17(12):1039-48
references.pmid: 8634065
references.citation: Am J Vet Res. 1997 Mar;58(3):286-92
references.pmid: 9055976
references.citation: J Cell Biochem. 2000 Sep 14;79(4):519-31
references.pmid: 10996843
references.citation: Osteoarthritis Cartilage. 2002 Oct;10(10):799-807
references.pmid: 12359166
references.citation: J Orthop Res. 1998 Jul;16(4):448-54
references.pmid: 9747786
references.citation: Matrix Biol. 1999 Jun;18(3):225-37
references.pmid: 10429942
references.citation: Biochem J. 1985 May 1;227(3):869-78
references.pmid: 3873936
references.citation: Cytokine. 2001 Oct 7;16(1):10-21
references.pmid: 11669582
references.citation: Osteoarthritis Cartilage. 2006 Feb;14(2):120-30
references.pmid: 16242973
references.citation: Matrix Biol. 2002 Apr;21(3):271-88
references.pmid: 12009333
references.citation: Arthritis Rheum. 2000 Mar;43(3):664-72
references.pmid: 10728761
references.citation: J Rheumatol. 1995 Sep;22(9):1740-6
references.pmid: 8523355
references.citation: Arthritis Rheum. 2002 Jan;46(1):124-9
references.pmid: 11817583
references.citation: J Small Anim Pract. 2006 Aug;47(8):439-45
references.pmid: 16911111
references.citation: Am J Vet Res. 2005 Oct;66(10):1679-85
references.pmid: 16273897
references.citation: J Small Anim Pract. 2005 Sep;46(9):425-9
references.pmid: 16167592
references.citation: Cytokine. 1996 Jun;8(6):495-500
references.pmid: 8818547
references.citation: Osteoarthritis Cartilage. 2005 Mar;13(3):235-42
references.pmid: 15727890
references.citation: J Biomech. 1995 Aug;28(8):977-83
references.pmid: 7673264
references.citation: Arthritis Rheum. 1998 Oct;41(10):1760-71
references.pmid: 9778217
references.citation: Biochim Biophys Acta. 1986 Sep 4;883(2):173-7
references.pmid: 3091074
references.citation: J Biomech. 2003 Apr;36(4):553-68
references.pmid: 12600346
references.citation: J Biol Chem. 1996 Jan 19;271(3):1544-50
references.pmid: 8576151
references.citation: Biorheology. 1981;18(3-6):619-32
references.pmid: 6799013
references.citation: J Clin Invest. 1996 Feb 1;97(3):761-8
references.pmid: 8609233
references.citation: Vet Rec. 2001 Feb 17;148(7):204-6
references.pmid: 11265997
references.citation: Rheumatology (Oxford). 2006 Aug;45(8):958-65
references.pmid: 16467367
references.citation: Nature. 2005 Mar 31;434(7033):648-52
references.pmid: 15800625
references.citation: Biomech Model Mechanobiol. 2006 Jun;5(2-3):64-81
references.pmid: 16534622
references.citation: Clin Exp Rheumatol. 2008 Jan-Feb;26(1):139-45
references.pmid: 18328163
references.citation: Osteoarthritis Cartilage. 1998 May;6(3):214-28
references.pmid: 9682788