title: The polarized distribution of Na+,K+-ATPase: role of the interaction between {beta} subunits.
abstract: The very existence of higher metazoans depends on the vectorial transport of substances across epithelia. A crucial element of this transport is the membrane enzyme Na(+),K(+)-ATPase. Not only is this enzyme distributed in a polarized manner in a restricted domain of the plasma membrane but also it creates the ionic gradients that drive the net movement of glucose, amino acids, and ions across the entire epithelium. In a previous work, we have shown that Na(+),K(+)-ATPase polarity depends on interactions between the beta subunits of Na(+),K(+)-ATPases located on neighboring cells and that these interactions anchor the entire enzyme at the borders of the intercellular space. In the present study, we used fluorescence resonance energy transfer and coprecipitation methods to demonstrate that these beta subunits have sufficient proximity and affinity to permit a direct interaction, without requiring any additional extracellular molecules to span the distance.
authors.affiliation: Department of Physiology Biophysics and Neurosciences, and Department of Molecular Biomedicine, Center for Research and Advanced Studies, CINVESTAV-IPN, Mexico DF 07300, Mexico.
authors.forename: Teresita
authors.initials: T
authors.lastname: Padilla-Benavides
authors.forename: María L
authors.initials: ML
authors.lastname: Roldán
authors.forename: Isabel
authors.initials: I
authors.lastname: Larre
authors.forename: David
authors.initials: D
authors.lastname: Flores-Benitez
authors.forename: Nicolas
authors.initials: N
authors.lastname: Villegas-Sepúlveda
authors.forename: Ruben G
authors.initials: RG
authors.lastname: Contreras
authors.forename: Marcelino
authors.initials: M
authors.lastname: Cereijido
authors.forename: Liora
authors.initials: L
authors.lastname: Shoshani
mesh_terms: D000818:Animals; D002460:Cell Line; D016764:Cell Polarity; D006224:Cricetinae; D003412:Cricetulus; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D020033:Protein Isoforms; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D000254:Sodium-Potassium-Exchanging ATPase
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