Branched arabino-oligosaccharides isolated from sugar beet arabinan.
Sugar beet arabinan consists of an alpha-(1,5)-linked backbone of L-arabinosyl residues, which can be either single or double substituted with alpha-(1,2)- and/or alpha-(1,3)-linked L-arabinosyl residues. Neutral branched arabino-oligosaccharides were isolated from sugar beet arabinan by enzymatic degradation with mixtures of pure and well-defined arabinohydrolases from Chrysosporium lucknowense followed by fractionation based on size and analysis by MALDI-TOF MS and HPAEC. Using NMR analysis, two main series of branched arabino-oligosaccharides have been identified, both having an alpha-(1,5)-linked backbone of L-arabinosyl residues. One series carries single substituted alpha-(1,3)-linked L-arabinosyl residues at the backbone, whereas the other series consists of a double substituted alpha-(1,2,3,5)-linked arabinan structure within the molecule. The structures of eight such branched arabino-oligosaccharides were established.
Westphal
Yvonne
Y
Wageningen University, Laboratory of Food Chemistry, Wageningen, The Netherlands.
Kühnel
Stefan
S
de Waard
Pieter
P
Hinz
Sandra W A
SW
Schols
Henk A
HA
Voragen
Alphons G J
AG
Gruppen
Harry
H
D001089:Arabinose / Q000737:chemistry*; D027461:Beta vulgaris / Q000737:chemistry; D002240:Carbohydrate Sequence; D002912:Chrysosporium / Q000201:enzymology; D019281:Dimerization; D006867:Hydrolases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*
D009844:Oligosaccharides; D011134:Polysaccharides; C030080:araban; D001089:Arabinose; D006867:Hydrolases