title: [Serotyping and genotypic characteristic of Vibrio cholerae non-O1/non-O139 serogroups isolated from water of surface basins and sewages of Rostov-on-Don city in 2003 - 2008].
abstract: OBJECTIVE
  Determination of serogroup and PCR-genotyping of Vibrio cholerae non-O1/non-O139 strains isolated from surface basins and sewages of Rostov-on-Don city in 2003 - 2008.
  
  
  METHODS
  Seven hundred strains of V. cholerae non-O1/non-O139 serogroups were studied in reaction of slide-agglutination with array of 80 diagnostic sera for non-O1/non-O139 serogroups. Selective screening of strains representing dominating serogroups was performed for extended number of genetic determinants of pathogenicity factors.
  
  
  RESULTS
  It was established that V. cholerae belonging to serogroups O53, O67, O75, and O76 are dominating in water ecosystems of Rostov-on-Don city at this time. All studied strains were characterized by lack of cholera toxin genes and toxin-coregulated pili but had different combinations of genes of additional virulence factors. There was no correlation between genotypic characteristics and serogroup.
  
  
  CONCLUSIONS
  The study showed that change of serologic landscape of V. cholerae non-O1/non-O139 occurred in water objects in studied area during last decades. Necessity of dynamic surveillance for circulation of V. cholerae non-O1/non-O139 in aquatic environment with widening of studied spectrum of their biological features was demonstrated.
authors.forename: V D
authors.initials: VD
authors.lastname: Kruglikov
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Lomov
authors.forename: E P
authors.initials: EP
authors.lastname: Avdeeva
authors.forename: E V
authors.initials: EV
authors.lastname: Monakhova
authors.forename: M I
authors.initials: MI
authors.lastname: Ezhova
authors.forename: I V
authors.initials: IV
authors.lastname: Arkhangel'skaia
authors.forename: I S
authors.initials: IS
authors.lastname: Shestialtynova
authors.forename: E G
authors.initials: EG
authors.lastname: Tsedova
authors.forename: O A
authors.initials: OA
authors.lastname: Shalu
authors.forename: N N
authors.initials: NN
authors.lastname: Uskova
authors.forename: L V
authors.initials: LV
authors.lastname: Grigorenko
mesh_terms: D002771:Cholera / Q000453:epidemiology / Q000821:virology*; D002772:Cholera Toxin / Q000235:genetics; D004784:Environmental Monitoring; D062665:Epidemiological Monitoring; D010861:Fimbriae, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D012426:Russia / Q000453:epidemiology; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D041242:Vibrio cholerae O139 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D044163:Vibrio cholerae non-O1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics; D014871:Water Microbiology
chemical_list: D037521:Virulence Factors; D002772:Cholera Toxin