Immunohistochemical and molecular detection of equine herpesvirus 1 in Uruguay.
Equine herpesvirus 1 (EHV-1) is a major cause of epidemic abortion, neonatal mortality, respiratory disease and neurological disorders in horses. In South America, the virus has been isolated in Brazil, Argentina and Colombia. In Chile pathological findings from one aborted foetus have been reported, and in Uruguay only serological data about EHV-1 activity have been found. Some pathological findings were reported in Uruguay several years ago, but these data have never been officially confirmed. The present work describes the relevant findings of a study of EHV-1 infections in the Uruguayan equine population using polymerase chain reaction (PCR) and histological and immunohistochemical analysis techniques. The sequence analysis of a portion of the glycoprotein C gene amplified by PCR confirmed EHV-1 activity. The real-time PCR revealed the association of the virus with the non-neuropathogenic genotype. This study describes for the first time the immunohistochemical and molecular detection of EHV-1 in Uruguay.
Easton
C
C
Department of Pathobiology, Veterinary Laboratories Division (DILAVE) Miguel C. Rubino, General Direction of Livestock Services of the Ministry of Livestock, Agriculture and Fisheries (MGAP), Montevideo, Uruguay.
Fuentealba
N A
NA
Paullier
C
C
Alonzo
P
P
Carluccio
J
J
Galosi
C M
CM
D037881:Aborted Fetus / Q000473:pathology / Q000821:virology; D000034:Abortion, Veterinary / Q000821:virology; D000818:Animals; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000235:genetics / Q000302:isolation & purification*; D006734:Horse Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D006736:Horses; D007150:Immunohistochemistry / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D014583:Uruguay