PIASy interacts with p73alpha and regulates cell cycle in HEK293 cells.
p73, a recently described member of the p53 family of transcription factors, undergoes a number of posttranslational modifications, inducing cell cycle arrest and apoptosis. In the present study, we demonstrate that PIASy, a member of the PIAS SUMO-ligase family, interacts with p73alpha, and that PIASy-mediated sumoylation promotes proteasomal degradation of p73alpha. PIASy overexpression inhibits p73alpha-mediated transcription of p21, with a reduction of cells in G1 and cell cycle reentry. These results suggest that PIASy plays an important role in regulation of p73alpha transcriptional activity and is also a regulator of the cell cycle machinery.
Zhang
Chao
C
Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, PR China.
Yuan
Xia
X
Yue
Ling
L
Fu
Jin
J
Luo
Lan
L
Yin
Zhimin
Z
D002453:Cell Cycle / Q000502:physiology*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D015151:Immunoblotting; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D050828:Protein Inhibitors of Activated STAT / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D050828:Protein Inhibitors of Activated STAT; D025841:Small Ubiquitin-Related Modifier Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins