title: Partial depolymerization of pectin by a photochemical reaction.
abstract: Complex heterogeneous polysaccharides that comprise pectin were partially depolymerized by a photochemical reaction using ultraviolet light in the presence of titanium dioxide catalyst. In a period of 6h at pH 7, this UV/TiO(2) process decreased the average molecular weight of pectin from 400kDa to 200kDa. The characterization of the partially depolymerized pectin, which was fractionated by size-exclusion chromatography, was performed by (1)H NMR spectroscopy, and the spectra obtained showed that the resulting oligosaccharides and polysaccharides maintained the intact core structure of pectin. The monosaccharide content and depolymerization profile were determined by high-performance anion-exchange chromatography coupled with pulsed amperometric detection. This controlled photochemical depolymerization technique might be useful for preparation of pectin oligosaccharides as an ingredient in food and pharmaceutical products.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Silpakorn University, Nakorn-pathom, Thailand.
authors.forename: Jankana
authors.initials: J
authors.lastname: Burana-osot
authors.forename: Noppamas
authors.initials: N
authors.lastname: Soonthornchareonnon
authors.forename: Saori
authors.initials: S
authors.lastname: Hosoyama
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Toida
mesh_terms: D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004563:Electrochemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry; D010368:Pectins / Q000737:chemistry*; D055668:Photochemical Processes; D010782:Photolysis; D014025:Titanium / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D006603:Hexuronic Acids; D009844:Oligosaccharides; C009495:titanium dioxide; C007819:galacturonic acid; D010368:Pectins; D014025:Titanium