title: Characterization of a diguanylate cyclase from Shewanella woodyi with cyclase and phosphodiesterase activities.
abstract: Cyclic-di-GMP signaling is used by many bacteria to control biofilm formation. As biofilm formation is an important survival strategy for many bacteria, the synthesis and degradation of cyclic-di-GMP is tightly regulated by enzymes containing domains with conserved GGDEF and EAL sequence motifs, respectively. In this report we characterize a protein with both cyclase and phosphodiesterase activities and demonstrate that it contributes to secretion of the extracellular polysaccharide matrix, an important step in early biofilm formation.
authors.affiliation: Department of Chemistry, Stony Brook University, Stony Brook, NY 11790-3400, USA.
authors.forename: Niu
authors.initials: N
authors.lastname: Liu
authors.forename: Taemee
authors.initials: T
authors.lastname: Pak
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Boon
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism*; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics / Q000378:metabolism*; D020592:Shewanella / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D010727:Phosphoric Diester Hydrolases; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase