title: Age-related changes in nitric oxide synthase in the lateral geniculate nucleus of rats.
abstract: Age-related changes in nitric oxide production in the visual system have not been well characterized. Therefore, we used staining and image-processing approaches to describe changes in levels of neuronal nitric oxide synthase (nNOS), the NADPH-diaphorase (NADPH-d) histochemical marker, and 3-nitrotyrosine in the lateral geniculate nucleus (LGN) of young and aged rats. The LGN plays an important role in the visual system, as it acts as a visual relay nucleus. Quantitative analysis of NADPH-d-positive and nNOS-immunoreactive neurons revealed significant optical density increases in the dorsal LGN and ventral LGN of aged rats; however, no significant changes were observed in the number of neurons with age. 3-Nitrotyrosine immunoreactivity was increased in the dorsal LGN and ventral LGN of aged rats. These results indicate that increased nitric oxide production and peroxynitrite may be associated with alterations in visual function during aging.
authors.affiliation: Department of Anatomy and Cell Biology, University of Ulsan College of Medicine, Poongnap-Dong, Songpa-Gu, Seoul, South Korea.
authors.forename: Seung-Jun
authors.initials: SJ
authors.lastname: Hwang
authors.forename: Youngbuhm
authors.initials: Y
authors.lastname: Huh
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D005829:Geniculate Bodies / Q000201:enzymology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000201:enzymology / Q000648:ultrastructure; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: C002744:3-nitrotyrosine; D014443:Tyrosine; D052248:Nitric Oxide Synthase Type I; D009252:NADPH Dehydrogenase
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