title: Expression of interleukin 6 in brain and colon of rats with TNBS-induced colitis.
abstract: OBJECTIVE
  To characterise expression of interleukin 6 (IL-6), a potent proinflammatory cytokine, in the occurrence and development of inflammatory bowel disease (IBD) and investigate its effect on neuroimmunomodulation and immune homeostasis regulation.
  
  
  METHODS
  In this study, rats with colitis induced by trinitrobenzene sulfonic acid (TNBS) were sacrificed on days 3, 7, 14, 21 and 28 after induction. In the controls, the TNBS was just replaced by equivalent amount of phosphate buffered solution (PBS, 0.01 mol/L). IL-6 mRNA expression in brain and colon tissues in each phase was evaluated by real-time reverse transcription-polymerase chain reaction, and cellular localisation and protein level of IL-6 was determined by immunohistochemistry.
  
  
  RESULTS
  At day 7, mRNA expression of IL-6 was significantly higher in the colon and brain of IBD rats than that of the controls. The protein level was also significantly higher in colon, hypothalamus and cerebral cortex of IBD rats compared with the controls. So there are similar temporal trends in IL-6 mRNA expression and protein levels in all positions with a persistent increase to a peak at day 7, followed by a decline and gradual return to normal levels.
  
  
  CONCLUSIONS
  These results revealed that changes in IL-6 expression in brain and colon tissues occur in different phases of IBD. Therefore, we propose that the nerve centre regulates and controls the occurrence and development of IBD via IL-6.
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Avenue, Changchun 130062, Jilin Province, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Chuan-Ping
authors.initials: CP
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhan-Qing
authors.initials: ZQ
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.forename: Lian-Zhi
authors.initials: LZ
authors.lastname: Mu
authors.forename: Zhan-Peng
authors.initials: ZP
authors.lastname: Yue
authors.forename: Xiu-Ling
authors.initials: XL
authors.lastname: Yin
authors.forename: Zhong-Ming
authors.initials: ZM
authors.lastname: Hu
authors.forename: Ju-Xiong
authors.initials: JX
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000276:immunology; D003092:Colitis / Q000139:chemically induced / Q000235:genetics* / Q000276:immunology*; D003106:Colon / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D017931:DNA Primers / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000209:etiology; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D015213:Neuroimmunomodulation; D009195:Peroxidase / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity
chemical_list: D017931:DNA Primers; D015850:Interleukin-6; D012333:RNA, Messenger; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase
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