[Application of the method of vital in vivo fluorescence intensity measurement in biological tissues for pharmacokinetic studies of different chlorin-based photosensitisers].
A precise and reproducible method of quantitative determination of the photosensitizer Photolon in hepatic tissue of the liver of rats by means of spectrophotometric assay with preliminary chemical extraction was developed for validation of the method of in vivo measurement of a fluorescent signal of the tissue after injection of the photosensitizer. Using the ex vivo spectrophotometric technique a quantitative assessment of the results of spectrofluorescent measurements performed in vivo was made. A high correlation (R2=0.99) between results estimated by two different techniques was shown. Also validity of the simple and easy-to-use method of in vivo fluorescence intensity measurements for the investigation of the pharmacokinetics and biodistribution of different photosensitizers was demonstrated.
Shliakhtin
S V
SV
Trukhacheva
T V
TV
Isakov
G A
GA
Istomin
Iu P
IuP
D000818:Animals; D002735:Chlorophyllides; D005260:Female; D008099:Liver / Q000378:metabolism*; D008297:Male; D017319:Photosensitizing Agents / Q000493:pharmacokinetics*; D011166:Porphyrins; D011205:Povidone / Q000493:pharmacokinetics*; D011524:Protoporphyrins / Q000493:pharmacokinetics*; D051381:Rats
D002735:Chlorophyllides; D017319:Photosensitizing Agents; D011166:Porphyrins; D011524:Protoporphyrins; C062985:phytochlorin; D011205:Povidone