Subungual keratoacanthoma: analysis of cell proliferation and copy number variation of oncogenes compared with periungual squamous cell carcinoma.
BACKGROUND
Subungual keratoacanthoma (SUKA) is a rare cutaneous tumour with several features distinct from ordinary KA. SUKA may not show spontaneous regression and sometimes grows progressively, resulting in phalangeal bone destruction. This makes its distinction from digital squamous cell carcinoma (SCC) difficult. Aim.  To investigate differences in molecular expression between SUKA and digital SCC.


METHODS
In addition to immunohistochemical analysis of Ki-67, one of the markers differentiating KA from SCC, we investigated the copy numbers of various oncogenes by multiplex ligation-dependent probe amplification (MLPA) using two cases of SUKA and three cases of periungual SCC.


RESULTS
Ki-67 was moderately or strongly positive in SCC but negative in SUKA. The MLPA analysis showed that the nuclear factor (NF)κB1 and cortactin (CTTN; formerly known as EMS1) genes are amplified in SUKA but not in digital SCC. This increase in NFκB1 was confirmed by immunohistochemical analysis.


CONCLUSIONS
NFκB1 could be a novel marker to differentiate between SUKA and SCC. Although this study was performed on limited numbers of patients with SUKA, MLPA analysis could be applied to differentiate other benign tumours from their malignant counterparts.
Honma
M
M
Department of Dermatology, Asahikawa Medical College, Asahikawa, Japan. wanwan@asahikawa-med.ac.jp
Kato
N
N
Hashimoto
M
M
Takahashi
H
H
Ishida-Yamamoto
A
A
Iizuka
H
H
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D051356:Cortactin / Q000235:genetics / Q000378:metabolism; D003937:Diagnosis, Differential; D006801:Humans; D007636:Keratoacanthoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D052002:NF-kappa B p50 Subunit / Q000378:metabolism; D009260:Nail Diseases / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D009857:Oncogenes / Q000235:genetics*; D012878:Skin Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D014408:Biomarkers, Tumor; D051356:Cortactin; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human