[Enhancement of antiviral immunity in HBV mouse model by blocking PD-1/PD-L1 signaling pathway].
OBJECTIVE
To establish a mouse model for human chronic HBV infection, and to investigate the role of PD-1/PD-L1 signaling pathway in antiviral immunity.


METHODS
A mouse model was established by hydrodynamic injection of the plasmid pAAV/HBV1.2-GFP into the tail vein of C57BL/6 mice, HBV markers were assayed at different time points after injection. After intraperitoneal injection of anti-PD-L1 monoclonal antibody, the serum ALT, and HBV DNA in the serum, liver and kidney were assayed.


RESULTS
The chronic HBV infection mouse model were established successfully, serum HBsAg and high load of HBV DNA were detectable 90 days after plasmid injection. After blocking of the PD-1/PD-L1 pathway, the serum ALT level of mice were significantly increased (P < 0.01), and the HBV DNA load in serum (P < 0.01), liver (P < 0.05) and kidney (P < 0.05) were decreased significantly.


CONCLUSIONS
Blocking the PD-1/PD-L1 signaling pathway can enhance antiviral response in mice with chronic HBV infection.
Weng
Zhi-hong
ZH
Department of Infectious Diseases, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Ye
Pian
P
Zhang
Shu-ling
SL
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D018122:B7-1 Antigen / Q000378:metabolism*; D060890:B7-H1 Antigen; D004279:DNA, Viral / Q000032:analysis; D004195:Disease Models, Animal; D006515:Hepatitis B virus / Q000378:metabolism*; D019694:Hepatitis B, Chronic / Q000276:immunology / Q000378:metabolism / Q000821:virology*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000378:metabolism*; D061026:Programmed Cell Death 1 Receptor; D015398:Signal Transduction
D000954:Antigens, Surface; D051017:Apoptosis Regulatory Proteins; D018122:B7-1 Antigen; D060890:B7-H1 Antigen; C498919:Cd274 protein, mouse; D004279:DNA, Viral; D008562:Membrane Glycoproteins; C491383:Pdcd1 protein, mouse; D010455:Peptides; D061026:Programmed Cell Death 1 Receptor