NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrix.
NeuN is an antigen detected in the nucleus of neurons in a wide range of vertebrates and so it is widely used as a tool for detecting neuronal cells. NeuN has been recently identified as Fox-3, a new member of the Fox-1 gene family of splicing factors. The predominant localization of NeuN/Fox-3 to neuronal nuclei and its role in splicing pose the question of the nuclear compartmentalization of such a protein. Here we provide evidence that NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrix and a reliable marker of nuclear speckles in neurons.
Dent
Myrna A R
MA
Facultad de Medicina, Universidad Autónoma del Estado de México, Toluca, México, Mexico.
Segura-Anaya
Edith
E
Alva-Medina
Janeth
J
Aranda-Anzaldo
Armando
A
D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism*; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D015530:Nuclear Matrix / Q000378:metabolism*; D051381:Rats
D034961:Antigens, Nuclear; D009419:Nerve Tissue Proteins; C546339:Rbfox3 protein, rat