title: Association of -2548 G/A polymorphism in the leptin gene with preeclampsia/pregnancy-induced hypertension.
abstract: OBJECTIVE
  To investigate −2548 G/A polymorphism of LEP gene, plasma leptin and soluble leptin receptor in preeclampsia/pregnancy induced hypertension.
  
  
  METHODS
  Sixty-two patients and 63 controls (normal pregnancies) studied in the third trimester. Leptin and soluble leptin receptor measured by enzyme immunosorbent assay and −2548 G/A polymorphism by polymerase chain reaction followed by restriction enzyme digestion.
  
  
  RESULTS
  Frequency of the AA genotype was significantly (p < 0.001) higher in patients. The A allele conferred a relative risk of 1.67 for the disease (p < 0.0001). Preeclampsia/pregnancy induced hypertension significantly (p < 0.001 to 0.0001) affected leptin and soluble leptin receptor concentrations, free leptin index and leptin normalized to body mass index. Genotype significantly (p < 0.05) influenced only the soluble leptin receptor concentrations.
  
  
  CONCLUSIONS
  Preeclampsia/pregnancy induced hypertension appears to be associated with higher circulating leptin and lower SLR levels, and with the AA genotype of −2548 G/A polymorphism of the leptin gene.
authors.affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Sri Lanka.
authors.forename: B H K R
authors.initials: BH
authors.lastname: Sugathadasa
authors.forename: K H
authors.initials: KH
authors.lastname: Tennekoon
authors.forename: E H
authors.initials: EH
authors.lastname: Karunanayake
authors.forename: J M
authors.initials: JM
authors.lastname: Kumarasiri
authors.forename: A P D S
authors.initials: AP
authors.lastname: Wijesundere
mesh_terms: D000328:Adult; D000483:Alleles; D000704:Analysis of Variance; D005260:Female; D005787:Gene Frequency; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000097:blood / Q000235:genetics*; D020738:Leptin / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D054411:Receptors, Leptin / Q000097:blood / Q000235:genetics; D018709:Statistics, Nonparametric
chemical_list: D020738:Leptin; D054411:Receptors, Leptin