title: [No correlation between the sensitivity to 5-aza-dC and the global DNA methylation level in hepatocellular carcinoma cells].
abstract: OBJECTIVE
  To compare the sensitivity of different hepatocellular carcinoma (HCC) cell lines (HepG2, QGY7701, HepG2.2.15) and the normal liver cell line L02 to 5-aza-dC, an DNA methyltransferase inhibitor, and to explore the relationship between global DNA methylation level and the sensitivity to 5-aza-dC.
  
  
  METHODS
  HepG2, QGY7701, HepG2.2.15 and L02 cells were treated with 5-aza-dC at different concentration, cell proliferation was measured by MTT method, cell apoptosis was detected by measuring caspase 3 activity and cellular DNA fragmentation ELISA.
  
  
  RESULTS
  Compared to HepG2 and QGY7701 cells, HepG2.2.15 were less sensitive to the treatment of 5-aza-dC; the normal liver cell line L02 was less sensitive to 5-aza-dC than the HCC cell lines.
  
  
  CONCLUSIONS
  The sensitivity to 5-aza-dC of HCC cell lines and normal liver cells is not correlated with the global DNA methylation level.
authors.affiliation: Department of Gastroenterology, the Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing, China.
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Li
authors.forename: Ai-long
authors.initials: AL
authors.lastname: Huang
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D019175:DNA Methylation / Q000187:drug effects*; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors*; D000077209:Decitabine; D056945:Hep G2 Cells; D006801:Humans
chemical_list: D000077209:Decitabine; D015254:DNA Modification Methylases; D053148:Caspase 3; D001374:Azacitidine