The expression of LDL receptor in vessels with blood-brain barrier impairment in a stroke-prone hypertensive model.
We previously reported that the blood-brain barrier (BBB) function was deteriorated in vessels located in the hippocampus in stroke-prone spontaneously hypertensive rats (SHRSP). In order to assess whether substances with oxidative stress such as amyloid-beta (Abeta) can be scavenged in the BBB-damaged vessels, we examined the gene expression of representative efflux and influx transporters of Abeta, such as low-density lipoprotein receptor (LDLR), LDL-related protein 1 (LRP1), and the receptor for advanced glycation end product (RAGE) in the hippocampus of SHRSP with the BBB impairment and Wistar Kyoto rats (WKY) without the impairment. Real-time quantitative reverse transcriptase-polymerase chain reaction analysis revealed that LDLR gene expression was increased in the samples of SHRSP compared with those of WKY, while there was no significant difference in LRP1 or RAGE gene expression between SHRSP and WKY. Western blot analysis revealed that the protein expression of LDLR was increased in the samples of SHRSP compared with those of WKY. Immunoelectron microscopic examination revealed that the LDLR expression was seen in the luminal and abluminal cytoplasmic membranes and vesicular structures of the endothelial cells and the cytoplasm of perivascular cells, especially in vessels with immunoreactivity of albumin showing increased vascular permeability. These findings suggest that the expression of LDLR was increased in the hippocampus of SHRSP compared with that of WKY and was seen in the luminal and abluminal cytoplasmic membranes and vesicular structures of endothelial cells, suggesting a role of LDLR in the vessels with BBB impairment.
Ueno
Masaki
M
Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Kita-gun, Kagawa, Japan. masaueno@med.kagawa-u.ac.jp
Wu
Bin
B
Nakagawa
Toshitaka
T
Nagai
Yukiko
Y
Onodera
Masayuki
M
Huang
Cheng-Long
CL
Kusaka
Takashi
T
Kanenishi
Kenji
K
Sakamoto
Haruhiko
H
D000818:Animals; D001812:Blood-Brain Barrier / Q000473:pathology; D015153:Blotting, Western; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020521:Stroke
D012333:RNA, Messenger; D011973:Receptors, LDL
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