Optimization of medium for one-step fermentation of inulin extract from Jerusalem artichoke tubers using Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol.
The medium for one-step fermentation of raw inulin extract from Jerusalem artichoke tubers by Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol (R,R-2,3-BD) was developed. Inulin, K(2)HPO(4) and NH(4)Cl were found to be the key factors in the fermentation according to the results obtained from the Plackett-Burman experimental design. The optimal concentration range of the three factors was examined by the steepest ascent path, and their optimal concentration was further investigated according to the Box-Behnken design and determined to be 77.14 g/L, 3.09 g/L and 0.93 g/L, respectively. Under the optimal conditions, the concentration of the obtained R,R-2,3-BD was 36.92 g/L, at more than 98% optical purity. Compared with other investigated carbon resources, fermentation of the raw inulin extract afforded the highest yield of R,R-2,3-BD. This process featured one-step fermentation of inulin without further hydrolyzing, which greatly decreased the raw material cost and thus facilitated its practical application.
Gao
Jian
J
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, Nanjing 210009, PR China.
Xu
Hong
H
Li
Qiu-jie
QJ
Feng
Xiao-hai
XH
Li
Sha
S
D002072:Butylene Glycols / Q000302:isolation & purification / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D003198:Computer Simulation; D006368:Helianthus / Q000382:microbiology*; D007328:Insulin / Q000378:metabolism*; D008954:Models, Biological; D056507:Paenibacillus / Q000145:classification / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D013045:Species Specificity
D002072:Butylene Glycols; D007328:Insulin; D010936:Plant Extracts