title: Theory of Polymer Entrapped Enzyme Ultramicroelectrodes: Fundamentals.
abstract: We have developed a theoretical description of the amperometric response of ultramicroelectrode (UME) biosensors formed via enzyme entrapment. Our model allows for multiple enzymes and co-substrates, and results in a closed-form analytical expression for the steady-state current response of the disk ultramicroelectrode. It captures the effects of enzyme-entrapment domain size, species transport properties (which can be different in the polymer and surrounding electrolyte), enzyme kinetics, and axisymmetric diffusion. Assumptions inherent in the derivation are carefully explained, as are the resulting limits on the applicability of the results. The ability to theoretically predict the response of enzyme entrapped UMEs should enable improved design, operation, and data interpretation for this important class of biosensors.
authors.affiliation: Georgia Institute of Technology, G. W. Woodruff School of Mechanical Engineering, Atlanta, GA 30332-0405.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Kottke
authors.forename: Christine
authors.initials: C
authors.lastname: Kranz
authors.forename: Yong Koo
authors.initials: YK
authors.lastname: Kwon
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Masson
authors.forename: Boris
authors.initials: B
authors.lastname: Mizaikoff
authors.forename: Andrei G
authors.initials: AG
authors.lastname: Fedorov
references.citation: Anal Chem. 2002 Jan 15;74(2):355-61
references.pmid: 11811408
references.citation: Anal Chim Acta. 1973 May;64(3):439-55
references.pmid: 4701057
references.citation: J Am Chem Soc. 2006 May 10;128(18):6014-5
references.pmid: 16669652
references.citation: Biosens Bioelectron. 1997 Dec 30;12(12):1157-67
references.pmid: 9474766
references.citation: Philos Trans R Soc Lond B Biol Sci. 1987 Aug 28;316(1176):85-94
references.pmid: 2889233
references.citation: Anal Chem. 2005 May 15;77(10):3267-73
references.pmid: 15889918
references.citation: Science. 2006 Mar 17;311(5767):1570-4
references.pmid: 16543451
references.citation: Anal Chem. 2002 Jan 15;74(2):362-7
references.pmid: 11811409
references.citation: Anal Chem. 2006 May 1;78(9):3138-43
references.pmid: 16643005
references.citation: Biosens Bioelectron. 2006 Oct 15;22(4):461-72
references.pmid: 16797958
references.citation: Bioelectrochemistry. 2004 Jun;63(1-2):207-15
references.pmid: 15110274
references.citation: Trends Biotechnol. 2005 Aug;23(8):420-8
references.pmid: 15950302
references.citation: Mini Rev Med Chem. 2005 Mar;5(3):231-9
references.pmid: 15777257
references.citation: Anal Chem. 1987 Oct 1;59(19):2339-44
references.pmid: 3674445
references.citation: Biosens Bioelectron. 2003 Mar;18(2-3):141-9
references.pmid: 12485760
references.citation: Biosens Bioelectron. 2004 May 15;19(10):1301-7
references.pmid: 15046763