title: Genome-wide expression changes in Saccharomyces cerevisiae in response to high-LET ionizing radiation.
abstract: To understand the yeast response to high-linear energy transfer (LET) ionizing radiation (IR), we investigated global gene expression in yeast irradiated by three types of high-LET IR (fast neutrons, heavy ions, and thermal neutrons) and gamma rays using DNA microarray analysis. Stationary cells were irradiated by each IR and recultured in yeast-peptone-dextrose medium to allow repair for 40 min. RNA was then isolated from three independent samples of irradiated yeast. Genes involved in the Mec1p kinase pathway, which functions in DNA damage response, were induced by all forms of high-LET IR and by gamma rays. Some genes related to oxidative stress and the cell wall were induced by all forms of high-LET IRs. Gene expression patterns as a function of each type of high-LET IR were examined statistically by one-way analysis of variance. This analysis demonstrated the existence of irradiation-specific responses. For example, genes involved in ribosomal DNA synthesis were specifically induced by fast neutron irradiation, while the ubiquitin-proteasome system and heat shock response were specifically induced by thermal neutron irradiation. The study characterizes high-LET IR-induced gene expression and provides a molecular understanding of subsequent adaptation in yeast.
authors.affiliation: Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology, 3A Tsukuba West, 16-1, Onogawa, Tsukuba, Ibaraki 305-8569, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Mizukami-Murata
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Iwahashi
authors.forename: Shinzou
authors.initials: S
authors.lastname: Kimura
authors.forename: Kumie
authors.initials: K
authors.lastname: Nojima
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sakurai
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saitou
authors.forename: Noriko
authors.initials: N
authors.lastname: Fujii
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Murata
authors.forename: Shinzi
authors.initials: S
authors.lastname: Suga
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Kitagawa
authors.forename: Kenichi
authors.initials: K
authors.lastname: Tanaka
authors.forename: Satoru
authors.initials: S
authors.lastname: Endo
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hoshi
mesh_terms: D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D020411:Oligonucleotide Array Sequence Analysis; D011839:Radiation, Ionizing; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D029701:Saccharomyces cerevisiae Proteins