Novel 12-bp deletion in the coding region of the bovine NPM1 gene affects growth traits.
The nucleophosmin 1 gene (NPM1) encodes a multifunctional nucleolar phosphoprotein that plays a crucial role in the control of various aspects of cell growth and homeostasis. In this study, the coding region of the NPM1 gene was screened in 1035 individuals of 4 Chinese cattle breeds by DNA sequencing and polyacrylamide gel electrophoresis. A novel 12-bp deletion mutation was identified in the coding region of the NPM1 gene. The PCR products of primer NPM1-P2 exhibited 3 genotypes and 2 alleles: 178 bp (denoted as W) and 166 bp (denoted as D). Genotype DD and allele D were predominant in the studied populations. Association analysis with growth traits in the Nanyang breed (N = 265) showed that the animals with genotype DD had significantly greater birth weight, body weight, body length, and heart girth than those with genotype WD (P < 0.01 or P < 0.05) at birth and after 6 months and 12 months, but not at 18 and 24 months of age. Results of this study suggest that the NPM1 gene is a candidate gene for growth traits in cattle.
Huang
Y-Z
YZ
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, China.
Zhang
E-P
EP
Chen
H
H
Wang
J
J
Li
Z-J
ZJ
Huai
Y-T
YT
Ma
L
L
Lan
X-Y
XY
Ren
G
G
Lei
C-Z
CZ
Fang
X-T
XT
Wang
J-Q
JQ
D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D049628:Body Size / Q000235:genetics; D001835:Body Weight / Q000235:genetics; D002417:Cattle / Q000235:genetics* / Q000254:growth & development; D002455:Cell Division / Q000235:genetics; D017931:DNA Primers; D006580:Genetic Carrier Screening; D005838:Genotype; D006128:Growth / Q000235:genetics*; D006321:Heart / Q000033:anatomy & histology; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D000090243:Nucleophosmin; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017384:Sequence Deletion
D017931:DNA Primers; D009687:Nuclear Proteins; D000090243:Nucleophosmin
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