One year variability of peak heights, heterozygous balance and inter-locus balance for the DNA positive control of AmpFℓSTR© Identifiler© STR kit.
Multivariate analyses of 205 positive control experiments in an AmpFℓSTR© Identifiler© STR kit were used to analyze the factors affecting peak heights at 16 loci. Peak heights were found to be highly correlated between loci and there was evidence for a difference in sensitivity of the two genetic analyzers in the blue channel. Heterozygous balance response at 10 loci was found to behave as a random variable following a beta-distribution with typical median values of 90%, without locus or genetic analyzer effect. Inter-locus balance at 16 loci was influenced by the blue channel effect and a temporal switch of unexplained origin. The implications of these results for the choice of minimum threshold values in quality control are discussed.
Debernardi
A
A
Institut National de Police Scientifique, Laboratoire de Police Scientifique de Lyon, Ecully, France. alexandra.debernardi@voila.fr
Suzanne
E
E
Formant
A
A
Pène
L
L
Dufour
A B
AB
Lobry
J R
JR
D000483:Alleles; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D035061:Control Groups; D004247:DNA / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods; D056426:Genetic Loci; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000502:physiology; D006579:Heterozygote; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D015999:Multivariate Analysis; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic; D004247:DNA