title: Ultrasound-array-based real-time photoacoustic microscopy of human pulsatile dynamics in vivo.
abstract: With a refined ultrasound-array-based real-time photoacoustic microscopy (UA-PAM) system, we demonstrate the feasibility of noninvasive in vivo imaging of human pulsatile dynamics. The system, capable of real-time B-scan imaging at 50 Hz and high-speed 3-D imaging, is validated by imaging the subcutaneous microvasculature in rats and humans. After the validation, a human artery around the palm-wrist area is imaged, and its pulsatile dynamics, including the arterial pulsatile motion and changes in hemoglobin concentration, is monitored with 20-ms B-scan imaging temporal resolution. To our knowledge, this is the first demonstration of real-time photoacoustic imaging of human physiological dynamics. Our results show that UA-PAM can potentially enable many new possibilities for studying functional and physiological dynamics in both preclinical and clinical imaging settings.
authors.affiliation: Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Song
authors.forename: Konstantin
authors.initials: K
authors.lastname: Maslov
authors.forename: K Kirk
authors.initials: KK
authors.lastname: Shung
authors.forename: Lihong V
authors.initials: LV
authors.lastname: Wang
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D008833:Microcirculation / Q000502:physiology*; D033401:Microscopy, Acoustic / Q000295:instrumentation*; D011673:Pulsatile Flow / Q000502:physiology*; D051381:Rats; D015203:Reproducibility of Results; D012212:Rheology / Q000295:instrumentation*; D012680:Sensitivity and Specificity
references.citation: Nat Biotechnol. 2006 Jul;24(7):848-51
references.pmid: 16823374
references.citation: J Biomed Opt. 2008 Sep-Oct;13(5):054028
references.pmid: 19021408
references.citation: Opt Express. 2008 Oct 27;16(22):18551-6
references.pmid: 18958134
references.citation: Science. 1990 Apr 6;248(4951):73-6
references.pmid: 2321027
references.citation: Eur Radiol. 2003 Oct;13(10):2323-37
references.pmid: 12942278
references.citation: Opt Express. 2008 May 26;16(11):7915-28
references.pmid: 18545502
references.citation: Science. 1991 Nov 22;254(5035):1178-81
references.pmid: 1957169
references.citation: Phys Med Biol. 2006 Jul 7;51(13):R83-98
references.pmid: 16790922
references.citation: Nat Biotechnol. 2003 Jul;21(7):803-6
references.pmid: 12808463