title: Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish.
abstract: OBJECTIVE
  To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development.
  
  
  RESULTS
  In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates.
  
  
  CONCLUSIONS
  These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.
authors.affiliation: Vesalius Research Center, VIB, Leuven, Belgium.
authors.forename: Ilse
authors.initials: I
authors.lastname: Geudens
authors.forename: Robert
authors.initials: R
authors.lastname: Herpers
authors.forename: Karlien
authors.initials: K
authors.lastname: Hermans
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Segura
authors.forename: Carmen
authors.initials: C
authors.lastname: Ruiz de Almodovar
authors.forename: Jeroen
authors.initials: J
authors.lastname: Bussmann
authors.forename: Frederik
authors.initials: F
authors.lastname: De Smet
authors.forename: Wouter
authors.initials: W
authors.lastname: Vandevelde
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Hogan
authors.forename: Arndt
authors.initials: A
authors.lastname: Siekmann
authors.forename: Filip
authors.initials: F
authors.lastname: Claes
authors.forename: John C
authors.initials: JC
authors.lastname: Moore
authors.forename: Anna Silvia
authors.initials: AS
authors.lastname: Pistocchi
authors.forename: Sonja
authors.initials: S
authors.lastname: Loges
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Mazzone
authors.forename: Giovanni
authors.initials: G
authors.lastname: Mariggi
authors.forename: Françoise
authors.initials: F
authors.lastname: Bruyère
authors.forename: Franco
authors.initials: F
authors.lastname: Cotelli
authors.forename: Dontscho
authors.initials: D
authors.lastname: Kerjaschki
authors.forename: Agnes
authors.initials: A
authors.lastname: Noël
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Foidart
authors.forename: Holger
authors.initials: H
authors.lastname: Gerhardt
authors.forename: Annelii
authors.initials: A
authors.lastname: Ny
authors.forename: Tobias
authors.initials: T
authors.lastname: Langenberg
authors.forename: Nathan D
authors.initials: ND
authors.lastname: Lawson
authors.forename: Henricus J
authors.initials: HJ
authors.lastname: Duckers
authors.forename: Stefan
authors.initials: S
authors.lastname: Schulte-Merker
authors.forename: Peter
authors.initials: P
authors.lastname: Carmeliet
authors.forename: Mieke
authors.initials: M
authors.lastname: Dewerchin
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015415:Biomarkers / Q000378:metabolism; D019556:COS Cells; D002465:Cell Movement; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D018920:Coculture Techniques; D004625:Embryo, Nonmammalian / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D042583:Lymphangiogenesis / Q000235:genetics; D008208:Lymphatic System / Q000196:embryology / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013897:Thoracic Duct / Q000196:embryology / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D047908:Intracellular Signaling Peptides and Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D051880:Receptors, Notch; D029961:Zebrafish Proteins; C064045:delta protein
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