Copper uptake and its effect on metal distribution in root growth zones of Commelina communis revealed by SRXRF.
To explore the copper uptake mechanisms by the Cu-tolerant plant Commelina communis, the contents of Cu and other metals (including Fe, Zn, and Mn) in roots were detected using atomic absorption spectrometer under transporter inhibitors, partial element deficiency, or Cu excess treatments, while distribution characters of Cu and other metals in root growth zones were investigated by synchrotron radiation X-ray fluorescence spectroscopy (SRXRF). Cu uptake was inhibited by the uncoupler DNP and P-type ATPase inhibitor Na(3)VO(4), not by the Ca(2+) ion channel inhibitor LaCl(3), suggesting that Cu could probably be assimilated actively by root and be related with P-type ATPase, but not through Ca(2+) ion channel. Fe or Zn deficiency could enhance Cu uptake, while 100 μM Cu inhibited Fe, Zn, and Mn accumulation in roots significantly. Metal distribution under 100 μM Cu treatment was investigated by SRXRF. High level of Cu was found in the root meristem, and higher Cu concentrations were observed in the vascular cylinder than those in the endodermis, further demonstrating the initiative Cu transport in the root of C. communis. Under excess Cu stress, most Fe was located in the epidermis, and Fe concentrations in the endodermis were higher than those in the vascular cylinder, suggesting Cu and Fe competition not only in the epidermal cells but also for the intercellular and intracellular transport in roots. Zn was present in the meristem and the vascular cylinder similar to Cu. Cu and Zn showed a similar pattern. Mn behaves as Zn does, but not like Fe.
Shi
Jiyan
J
Department of Environmental Engineering, Huajiachi Campus, Zhejiang University, 268 Kaixuan Road, Hangzhou, Zhejiang, 310029, China. shijiyan@zju.edu.cn
Yuan
Xiaofeng
X
Chen
Xincai
X
Wu
Bei
B
Huang
Yuying
Y
Chen
Yingxu
Y
D034822:Commelina / Q000187:drug effects* / Q000254:growth & development; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008670:Metals / Q000378:metabolism / Q000494:pharmacology; D018517:Plant Roots / Q000378:metabolism*; D013052:Spectrometry, X-Ray Emission / Q000379:methods*; D017356:Synchrotrons
D008670:Metals; D003300:Copper