title: Patterning of novel breast implant surfaces by enhancing silicone biocompatibility, using biomimetic topographies.
abstract: OBJECTIVE
  Silicone biocompatibility is dictated by cell-surface interaction and its understanding is important in the field of implantation. The role of surface topography and its associated cellular morphology needs investigation to identify qualities that enhance silicone surface biocompatability. This study aims to create well-defined silicone topographies and examine how breast tissue-derived fibroblasts react and align to these surfaces.
  
  
  METHODS
  Photolithographic microelectronic techniques were modified to produce naturally inspired topographies in silicone, which were cultured with breast tissue-derived human fibroblasts. Using light, immunofluorescent and atomic force microscopy, the cytoskeletal reaction of fibroblasts to these silicone surfaces was investigated.
  
  
  RESULTS
  Numerous, well-defined micron-sized pillars, pores, grooves, and ridges were manufactured and characterized in medical grade silicone. Inimitable immunofluorescent microscopy represented in our high magnification images of vinculin, vimentin, and the actin cytoskeleton highlights the differences in fibroblast adhesion between fabricated silicone surfaces. These unique figures illustrate that fibroblast adhesion and the reactions these cells have to silicone can be manipulated to enhance biointegration between the implant and the breast tissue. An alteration of fibroblast phenotype was also observed, exhibiting the propensity of these surfaces to induce categorical remodeling of fibroblasts.
  
  
  CONCLUSIONS
  This unique study shows that fibroblast reactions to silicone topographies can be tailored to induce physiological changes in cells. This paves the way for further research necessary to develop more biocompatible constructs capable of eliminating capsular contracture by subverting the foreign body response.
authors.affiliation: Plastic & Reconstructive Surgery Research, Manchester Interdisciplinary Biocentre, School of Translational Medicine, University of Manchester, Manchester, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Barr
authors.forename: E
authors.initials: E
authors.lastname: Hill
authors.forename: A
authors.initials: A
authors.lastname: Bayat
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