Multiple Myo4 motors enhance ASH1 mRNA transport in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae, ASH1 mRNA is transported to the bud tip by the class V myosin Myo4. In vivo, Myo4 moves RNA in a rapid and continuous fashion, but in vitro Myo4 is a nonprocessive, monomeric motor that forms a complex with She3. To understand how nonprocessive motors generate continuous transport, we used a novel purification method to show that Myo4, She3, and the RNA-binding protein She2 are the sole major components of an active ribonucleoprotein transport unit. We demonstrate that a single localization element contains multiple copies of Myo4 and a tetramer of She2, which suggests that She2 may recruit multiple motors to an RNA. Furthermore, we show that increasing the number of Myo4-She3 molecules bound to ASH1 RNA in the absence of She2 increases the efficiency of RNA transport to the bud. Our data suggest that multiple, nonprocessive Myo4 motors can generate continuous transport of mRNA to the bud tip.
Chung
Sunglan
S
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA.
Takizawa
Peter A
PA
D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism*; D024701:Myosin Type V / Q000378:metabolism*; D034443:RNA Transport; D012331:RNA, Fungal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*
C098733:ASH1 protein, S cerevisiae; D003598:Cytoskeletal Proteins; C098732:Myo4 protein, S cerevisiae; D012331:RNA, Fungal; D012333:RNA, Messenger; D012097:Repressor Proteins; C111369:SHE4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D024701:Myosin Type V; D018995:Myosin Heavy Chains
Mol Cell Biol. 2005 Jun;25(11):4752-66
15899876
Proc Natl Acad Sci U S A. 2009 Oct 13;106(41):17320-4
19805065
Traffic. 2008 Aug;9(8):1256-67
18485054
EMBO J. 2000 Dec 1;19(23):6592-601
11101531
Eur J Cell Biol. 1999 Apr;78(4):278-87
10350216
RNA. 2007 May;13(5):625-42
17449729
EMBO J. 2002 Mar 1;21(5):1158-67
11867544
Genes Dev. 2004 Jun 15;18(12):1452-65
15198983
RNA. 2010 Mar;16(3):585-97
20075165
Biochim Biophys Acta. 1966 Feb 7;112(2):346-62
5329026
J Biol Chem. 2005 Oct 14;280(41):34702-8
16030012
Mol Cell. 2006 Feb 3;21(3):331-6
16455488
Cell. 2004 Nov 12;119(4):491-502
15537539
RNA. 2003 Nov;9(11):1383-99
14561888
J Biol Chem. 2008 Mar 14;283(11):6906-14
18175803
Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18005-10
16326802
Biol Cell. 2008 Feb;100(2):125-38
18199049
Cell. 2009 Aug 7;138(3):537-48
19665975
J Cell Biol. 2007 Sep 24;178(7):1193-206
17893244
J Cell Biol. 2009 Oct 5;187(1):53-60
19786577
Methods. 2001 Jul;24(3):218-29
11403571
Cell. 2009 Feb 20;136(4):719-30
19239891
Nature. 1990 Nov 22;348(6299):348-52
2174512
J Cell Biol. 2001 Apr 16;153(2):307-18
11309412
Yeast. 1998 Jul;14(10):953-61
9717241
J Cell Sci. 1999 May;112 ( Pt 10):1511-8
10212145
Nat Cell Biol. 2000 Jan;2(1):20-4
10620802
Trends Cell Biol. 2003 Sep;13(9):447-51
12946621
Curr Biol. 1999 Mar 25;9(6):337-40
10209099
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5273-8
10792032
Nature. 2003 Oct 16;425(6959):737-41
14562106
EMBO J. 2001 Aug 1;20(15):4041-54
11483508
Curr Biol. 2006 Jul 25;16(14):1447-52
16860745
EMBO J. 2000 Oct 16;19(20):5514-24
11032818
Cell. 1996 Mar 8;84(5):687-97
8625407
Biol Cell. 2005 Jan;97(1):75-86
15601259
Nature. 1997 Sep 4;389(6646):90-3
9288973
J Cell Biol. 2001 May 28;153(5):1121-6
11381095
Cell Mol Life Sci. 2007 Jan;64(2):171-80
17131056
Cell. 1995 Jun 2;81(5):769-80
7539720
EMBO J. 2004 Apr 21;23(8):1729-38
15044955
Mol Biol Cell. 2009 Jul;20(14):3414-21
19477930
Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):87-92
17190808
Cell. 2009 Nov 25;139(5):983-98
19945381
Curr Opin Struct Biol. 1996 Feb;6(1):53-61
8696973
Curr Opin Cell Biol. 2009 Feb;21(1):59-67
19179063
Mol Cell. 1998 Oct;2(4):437-45
9809065
Semin Cell Dev Biol. 2007 Apr;18(2):194-201
17324596
Cell. 2002 May 3;109(3):347-58
12015984
Curr Biol. 1999 Mar 25;9(6):333-6
10209102
Biophys J. 2008 Jan 15;94(2):532-41
17872957
J Cell Biol. 2002 Dec 23;159(6):971-82
12499354
Science. 1997 Jul 18;277(5324):383-7
9219698
RNA. 2009 Nov;15(11):2002-12
19710186
Nat Rev Mol Cell Biol. 2010 Feb;11(2):113-27
20094052
Methods Enzymol. 2007;429:299-321
17913629
Trends Cell Biol. 2008 Mar;18(3):105-11
18262421
Semin Cell Dev Biol. 2007 Apr;18(2):171-7
17398125
Science. 2002 Sep 27;297(5590):2263-7
12351789
Nature. 1996 Apr 18;380(6575):646-50
8602269
Biophys J. 2000 Feb;78(2):719-30
10653785
Genetics. 2000 Feb;154(2):713-24
10655224
Nat Rev Mol Cell Biol. 2005 May;6(5):363-75
15852043