[Inhibition effect of IL-1beta on calcium channels currents in cultured cortical neurons of rat].
Interleukin-1beta (IL-1beta) is an important proinflammatory cytokine that exert wide biological functions in the central nervous system (CNS). Voltage-gated Ca2+ channels in plasma membrane are essential components of Ca2+ signal whose change on pathological conditions is closely related to processes of diseases. Although both IL-1beta and Ca2+ channels have key roles in injury and disease of the brain, so far their relations have been reported in few studies. The effects of IL-1beta on Ca2+ channels in cultured cortical neurons of rats were investigated using patch-clamp recording in present research. Our results showed that both 10 and 50 ng/mL of IL-1beta treatment inhibit the Ca2+ currents in time and dose dependent manner, without the change of activation properties of voltage-gated Ca2+ channels (VGCC)s.
Zhou
Chen
C
College of Life Science, Hebei University, Baoding 071002, China. zezhou@sina.com
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D055724:Electrophysiological Phenomena; D005260:Female; D053583:Interleukin-1beta / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D053583:Interleukin-1beta; D002118:Calcium