title: Reference change values of blood analytes from physically active subjects.
abstract: The analysis of blood constituents allows the detection of various physiological or pathological states when their values are increased or decreased in relation to a well-defined reference group or to themselves if monitored longitudinally. In the latter case, it is important to know the reference change value (RCV) or critical difference, which defines the percentage change that should be exceeded-given the analytical and biological variations inherent to a particular test, in that there is a significant difference between the two consecutive measurements. Our objective was to calculate the biological variation, analytical variation and RCV of the biochemical and hematological parameters in subjects undergoing 4 months of regular aerobic training. Blood samples (10 mL) were collected monthly from 56 male subjects (17-19 years old). Creatine kinase and aspartate aminotransferase activities, total cholesterol, triglycerides, uric acid, C-reactive protein, alpha-1-acid glycoprotein, creatinine and urea concentrations were measured in sera using an Autolab Boehringer analyzer. Hemogram were obtained from total blood using KX-21 N SYSMEX equipment. The RCV values for leukocytes and all biochemical analytes were elevated compared to the literature values of sedentary subjects. On the other hand, the RCV values for red blood cell count were slightly lower in physically active than in sedentary individuals. Knowledge of analyte RCV values within physically active subjects should improve the sensitivity/specificity of the hematological and biochemical alterations induced by training or the use of recombinant form of erythropoietin through blood parameter analysis, particularly in cases of longitudinal monitoring.
authors.affiliation: Departamento de Bioquímica, Laboratório de Bioquímica do Exercício-LABEX, Instituto de Biologia, Universidade de Campinas-Unicamp, Cidade Universitária Zeferino Vaz, s/n, Barão Geraldo, Campinas, SP, CEP 13083-970, Brazil.
authors.forename: Lázaro Alessandro S
authors.initials: LA
authors.lastname: Nunes
authors.forename: René
authors.initials: R
authors.lastname: Brenzikofer
authors.forename: Denise Vaz
authors.initials: DV
authors.lastname: de Macedo
mesh_terms: D000293:Adolescent; D001219:Aspartate Aminotransferases / Q000097:blood; D057205:Automation, Laboratory; D015415:Biomarkers / Q000097:blood*; D001774:Blood Chemical Analysis / Q000592:standards*; D002097:C-Reactive Protein / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D003404:Creatinine / Q000097:blood; D015444:Exercise; D006403:Hematologic Tests; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D009961:Orosomucoid / Q000378:metabolism; D012016:Reference Values; D014280:Triglycerides / Q000097:blood; D014508:Urea / Q000097:blood; D014527:Uric Acid / Q000097:blood; D055815:Young Adult
chemical_list: D015415:Biomarkers; D009961:Orosomucoid; D014280:Triglycerides; D014527:Uric Acid; D014508:Urea; D002097:C-Reactive Protein; D002784:Cholesterol; D003404:Creatinine; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase
references.citation: Clin Chem. 2005 Nov;51(11):2145-50
references.pmid: 16155091
references.citation: Ann Clin Biochem. 1997 Jan;34 ( Pt 1):8-12
references.pmid: 9022883
references.citation: Ann Clin Biochem. 1998 Jan;35 ( Pt 1):80-4
references.pmid: 9463743
references.citation: Clin Chem. 1999 Mar;45(3):321-3
references.pmid: 10053031
references.citation: Scand J Clin Lab Invest. 2004;64(3):175-84
references.pmid: 15222627
references.citation: Atherosclerosis. 1982 Dec;45(3):259-66
references.pmid: 7159498
references.citation: J Clin Pathol. 2008 Apr;61(4):426-7
references.pmid: 18326016
references.citation: Clin Chem Lab Med. 1999 Jun;37(6):655-61
references.pmid: 10475074
references.citation: Clin Chem. 2001 Dec;47(12):2146-8
references.pmid: 11719479
references.citation: Clin Chim Acta. 2008 Jun;392(1-2):1-7
references.pmid: 18348869
references.citation: Haematologica. 2003 May;88(5):570-81
references.pmid: 12745277
references.citation: J Sci Med Sport. 2009 Jan;12(1):85-90
references.pmid: 18069060
references.citation: Clin Chem. 1974 Dec;20(12):1535-42
references.pmid: 4430131
references.citation: Scand J Clin Lab Invest. 1999 Nov;59(7):491-500
references.pmid: 10667686
references.citation: Sports Med. 2009;39(4):331-7
references.pmid: 19317520
references.citation: Free Radic Biol Med. 2004 Oct 1;37(7):1064-72
references.pmid: 15336322
references.citation: Crit Rev Clin Lab Sci. 1989;27(5):409-37
references.pmid: 2679660
references.citation: Clin Chem. 1983 Jan;29(1):25-30
references.pmid: 6848276
references.citation: Am J Clin Pathol. 1996 Dec;106(6):723-7
references.pmid: 8980347
references.citation: Clin Sci (Lond). 1991 Aug;81(2):147-52
references.pmid: 1653657
references.citation: J Nutr Biochem. 2007 Jun;18(6):357-71
references.pmid: 17156994
references.citation: Clin Chem Lab Med. 2004;42(7):758-64
references.pmid: 15327011
references.citation: Clin Chem. 1997 Jan;43(1):52-8
references.pmid: 8990222
references.citation: Clin Chem. 2009 Jan;55(1):52-8
references.pmid: 18988755
references.citation: J Clin Chem Clin Biochem. 1985 Feb;23(2):69-76
references.pmid: 4039358
references.citation: Arch Pathol Lab Med. 1994 May;118(5):496-500
references.pmid: 8192558
references.citation: Med Sci Sports Exerc. 2000 Feb;32(2):332-48
references.pmid: 10694114
references.citation: Am J Clin Pathol. 1989 Oct;92(4):465-70
references.pmid: 2801611
references.citation: Arch Pathol Lab Med. 1992 Aug;116(8):825-6
references.pmid: 1497462
references.citation: Clin Chem Lab Med. 2005;43(4):441-8
references.pmid: 15899662
references.citation: Haematologica. 2007 Aug;92(8):1143-4
references.pmid: 17650448
references.citation: Clin Chim Acta. 2009 Jan;399(1-2):109-11
references.pmid: 18848535
references.citation: Clin Chem. 1993 Jun;39(6):1012-22
references.pmid: 8504530
references.citation: Am J Clin Pathol. 1978 Jan;69(1):48-54
references.pmid: 563672
references.citation: Pathol Biol (Paris). 2004 Feb;52(1):43-9
references.pmid: 14761713
references.citation: Ann Clin Biochem. 2007 Jul;44(Pt 4):343-52
references.pmid: 17594781
references.citation: Clin Chem Lab Med. 2004;42(7):710-4
references.pmid: 15327004
references.citation: Sports Med. 2006;36(4):327-58
references.pmid: 16573358
references.citation: Clin Chim Acta. 2004 Aug 2;346(1):55-64
references.pmid: 15234636