Resistin in human colon cancer. Increased expression independently of resistin promoter C-180G genotype.
OBJECTIVE
To determine the relationship between resistin gene RETN C-180G variant and circulating resistin concentration in Saudi colon cancer patients.


METHODS
This case-control study was conducted in the Biochemistry Department, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia from April 2009 to December 2009. The serum concentration had been measured with enzyme-linked immunosorbent assay in 60 colon cancer patients and in 60 controls matched in gender and age. The single nucleotide polymorphism SNP C-180G was genotyped using polymerase chain reaction PCR and restriction fragment length polymorphism RFLP techniques.


RESULTS
We observed a significantly higher serum resistin level in colon cancer group compared with control group 19.44 +/- 8.46 versus 5.45 +/- 2.73 ng/ml; p=0.0001, with significant p=0.03 higher levels showed in women than in men in patients and controls. In patients, the heterozygous CG and homozygous GG genotype carriers showed higher p=0.08 levels of serum resistin compared to CC homozygous. This difference was not observed p=0.78 among SNP C-180G genotypes in control group.


CONCLUSIONS
Our result showed no association between the C-180G SNP and the serum resistin concentrations and suggests that the high resistin level in colon cancer patients may play an important role in colon cancer development.
Al-Harithy
Rowyda N
RN
Biochemistry Department, King Abdul- Aziz University, PO Box 40288, Jeddah 21499, Kingdom of Saudi Arabia. dr.alharithy@gmail.com
Al-Ghafari
Ayat B
AB
D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000097:blood / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D052243:Resistin / Q000097:blood* / Q000235:genetics; D012529:Saudi Arabia
C499624:RETN protein, human; D052243:Resistin