Development of an in vitro model of feline cartilage degradation.
Osteoarthritis is the most common arthropathy of mammalian species including cats. Cartilage degradation is central to the disorder and here we present, for the first time, an in vitro model of feline cartilage degradation which will be useful for further studies in this target species. Feline articular cartilage explant cultures were maintained for 28 days and in the presence of oncostatin M with and without interleukin (IL)-17, tumour necrosis factor (TNF), IL-1alpha, or IL-1beta. Media samples and digested cartilage explants were analysed for glycosaminoglycan (GAG) and collagen content. The combination of IL-1beta and OSM, both at 20 ng/ml, was able to promote GAG release to the greatest extent at 14 days. At 28 days, all groups showed relatively high release of GAG. At 14 days, only IL-1beta and OSM in combination were associated with a statistically significant increase in collagen release over and above control tissue. IL-1beta dose-response studies showed that an IL-1beta dose of 10 ng/ml promotes a statistically significant increase in GAG breakdown when used with OSM, and higher doses of IL-1beta did not result in significantly greater response. The model demonstrated both GAG and collagen degradation and will be of use for further understanding of feline cartilage metabolism and for screening of potential structure-modifying agents to be used in cats.
Gabriel
Natalie
N
Musculoskeletal Research Group, School of Veterinary Science, University of Liverpool, Leahurst Campus, Neston, Wirral CH64 7TE, UK.
Innes
John F
JF
Caterson
Bruce
B
Vaughan-Thomas
Anne
A
D000818:Animals; D002357:Cartilage Diseases / Q000378:metabolism / Q000662:veterinary*; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism; D002371:Cat Diseases / Q000378:metabolism*; D002415:Cats; D003094:Collagen / Q000187:drug effects / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D053583:Interleukin-1beta / Q000494:pharmacology; D008954:Models, Biological; D053683:Oncostatin M / Q000494:pharmacology; D010003:Osteoarthritis / Q000662:veterinary; D046509:Tissue Culture Techniques / Q000662:veterinary*
D006025:Glycosaminoglycans; D053583:Interleukin-1beta; D053683:Oncostatin M; D003094:Collagen
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