Rhizobium borbori sp. nov., aniline-degrading bacteria isolated from activated sludge.
Three aniline-degrading bacteria, strains DN316(T), DN316-1 and DN365, were isolated from activated sludge. According to 16S rRNA gene sequence-based phylogenetic analysis, the isolates belonged to the genus Rhizobium, with Rhizobium ( = Agrobacterium) radiobacter LMG 140(T) as the closest relative, with 96.5 % sequence similarity. Phylogenetic analysis of the representative strain DN316(T) using sequences of the glnA, thrC and recA genes and the 16S-23S intergenic spacer region confirmed the phylogenetic arrangement obtained from analysis of the 16S rRNA gene. DNA-DNA relatedness between DN316(T) and R. radiobacter LMG 140(T) was 43.7 %, clearly indicating that the representative strain DN316(T) represents a novel species. Phenotypic and biochemical characterization of the isolates and insertion sequence-PCR fingerprinting patterns showed several distinctive features that differentiated them from closely related species. The major components of the cellular fatty acids were C(18 : 1)ω7c (57.10 %), C(16 : 0) (11.31 %) and C(19 : 0) cyclo ω8c (10.13 %). Based on our taxonomic analysis, the three isolates from activated sludge represent a novel species of the genus Rhizobium, for which the name Rhizobium borbori sp. nov. is proposed. The type strain is DN316(T) ( = CICC 10378(T)  = LMG 23925(T)).
Zhang
Guo Xia
GX
Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, PR China.|Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
Ren
Sui Zhou
SZ
Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
Xu
Mei Ying
MY
Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
Zeng
Guo Qu
GQ
Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
Luo
Hui Dong
HD
Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
Chen
Jin Lin
JL
Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
Tan
Zhi Yuan
ZY
Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, PR China.
Sun
Guo Ping
GP
Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
D000814:Aniline Compounds / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D005974:Glutamate-Ammonia Ligase; D008969:Molecular Sequence Data; D058889:Molecular Typing; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
D000814:Aniline Compounds; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D011938:Rec A Recombinases; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase; C023650:aniline