Improved transformation of the halo-tolerant yeast Zygosaccharomyces rouxii by electroporation.
To improve the transformation efficiency of Zygosaccharomyces rouxii by electroporation, glycerol was added to the electroporation buffer and the cells were frozen at -80 degrees C. These alterations drastically increased transformation efficiency, and we found that competent cells can be preserved at -80 degrees C without decreasing their transformation efficiency for at least 30 d.
Watanabe
Jun
J
Manufacturing Division, Yamasa Corporation, Choushi, Chiba, Japan. j_watanabe@yamasa.com
Uehara
Kenji
K
Mogi
Yoshinobu
Y
Suzuki
Kazushi
K
Watanabe
Takeshi
T
Yamazaki
Tatsuo
T
D018274:Electroporation / Q000379:methods*; D005615:Freezing; D055049:Salt Tolerance; D014170:Transformation, Genetic; D020068:Zygosaccharomyces / Q000166:cytology* / Q000235:genetics* / Q000502:physiology