title: Host genetics in granuloma formation: human-like lung pathology in mice with reciprocal genetic susceptibility to M. tuberculosis and M. avium.
abstract: Development of lung granulomata is a hallmark of infections caused by virulent mycobacteria, reflecting both protective host response that restricts infection spreading and inflammatory pathology. The role of host genetics in granuloma formation is not well defined. Earlier we have shown that mice of the I/St strain are extremely susceptible to Mycobacterium tuberculosis but resistant to M. avium infection, whereas B6 mice show a reversed pattern of susceptibility. Here, by directly comparing: (i) characteristics of susceptibility to two infections in vivo; (ii) architecture of lung granulomata assessed by immune staining; and (iii) expression of genes encoding regulatory factors of neutrophil influx in the lung tissue, we demonstrate that genetic susceptibility of the host largely determines the pattern of lung pathology. Necrotizing granuloma surrounded by hypoxic zones, as well as a massive neutrophil influx, develop in the lungs of M. avium-infected B6 mice and in the lungs of M. tuberculosis-infected I/St mice, but not in the lungs of corresponding genetically resistant counterparts. The mirror-type lung tissue responses to two virulent mycobacteria indicate that the level of genetic susceptibility of the host to a given mycobacterial species largely determines characteristics of pathology, and directly demonstrate the importance of host genetics in pathogenesis.
authors.affiliation: Laboratory for Immunogenetics, Central Institute for Tuberculosis, Moscow, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Kondratieva
authors.forename: Nadya
authors.initials: N
authors.lastname: Logunova
authors.forename: Konstantin
authors.initials: K
authors.lastname: Majorov
authors.forename: Mikhail
authors.initials: M
authors.lastname: Averbakh
authors.forename: Alexander
authors.initials: A
authors.lastname: Apt
mesh_terms: D000818:Animals; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D006099:Granuloma / Q000382:microbiology* / Q000473:pathology*; D054884:Host-Pathogen Interactions / Q000235:genetics*; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D007249:Inflammation / Q000473:pathology; D007958:Leukocyte Count; D008168:Lung / Q000382:microbiology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009162:Mycobacterium avium / Q000502:physiology*; D009169:Mycobacterium tuberculosis / Q000502:physiology*; D009504:Neutrophils / Q000473:pathology; D010641:Phenotype; D014376:Tuberculosis / Q000235:genetics / Q000382:microbiology
chemical_list: D018925:Chemokines
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