Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of macrophage growth locus A (MglA) protein from Francisella tularensis.
Francisella tularensis, a potential bioweapon, causes a rare infectious disease called tularemia in humans and animals. The macrophage growth locus A (MglA) protein from F. tularensis associates with RNA polymerase to positively regulate the expression of multiple virulence factors that are required for its survival and replication within macrophages. The MglA protein was overproduced in Escherichia coli, purified and crystallized. The crystals diffracted to 7.5 A resolution at the Advanced Photon Source, Argonne National Laboratory and belonged to the hexagonal space group P6(1) or P6(5), with unit-cell parameters a = b = 125, c = 54 A.
Subburaman
Priadarsini
P
Macromolecular Crystallography Laboratory, National Cancer Institute, 1050 Boyles Street, Frederick, MD 21702, USA.
Austin
Brian P
BP
Shaw
Gary X
GX
Waugh
David S
DS
Ji
Xinhua
X
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D005604:Francisella tularensis / Q000737:chemistry*; D015870:Gene Expression
D001426:Bacterial Proteins
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