title: Relevance of oral experimental challenge with classical scrapie in sheep.
abstract: Oral inoculation is currently considered as the best approach to mimic natural TSE contamination in ruminants. In this study, we compared the timing of abnormal prion protein (PrP(Sc)) dissemination and accumulation in the organism of susceptible sheep either orally inoculated or naturally infected with classical scrapie. Both animal groups shared a similar PrP(Sc) dissemination scheme and accumulation dynamics in lymphoid tissues. However, orally challenged animals displayed an earlier neuro-invasion and a dramatically shorter incubation period than naturally exposed sheep. No differences were observed between the groups with regards to the neuro-invasion route. These results unambiguously indicate that oral inoculation can have an impact on both the earliness of neuro-invasion and the incubation period. They also support the statement that oral inoculation is a relevant model for investigating transmissible spongiform encephalopathy pathogenesis. Nevertheless, data obtained under such experimental conditions should be used with some caution.
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôtes-Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Tabouret
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôtes-Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Lacroux
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôtes-Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Lugan
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôtes-Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: Pierrette
authors.initials: P
authors.lastname: Costes
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôtes-Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Corbière
authors.affiliation: INRA domaine de Langlade, 31450 Pompertuzat, France.
authors.forename: Jean Louis
authors.initials: JL
authors.lastname: Weisbecker
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôtes-Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: François
authors.initials: F
authors.lastname: Schelcher
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôtes-Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Andréoletti
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008221:Lymphoid Tissue / Q000737:chemistry; D009420:Nervous System / Q000737:chemistry; D018620:PrPSc Proteins / Q000032:analysis; D012608:Scrapie / Q000635:transmission*; D012756:Sheep; D012757:Sheep Diseases / Q000635:transmission*; D013997:Time Factors
chemical_list: D018620:PrPSc Proteins