title: Anti-inflammatory eunicellin-based diterpenoids from the cultured soft coral Klyxum simplex.
abstract: Three novel eunicellin-based diterpenoids, namely klysimplexin sulfoxides A-C (1-3), were isolated from the cultured soft coral Klyxum simplex. Their structures were elucidated by spectroscopic methods, particularly 1D and 2D-NMR experiments. Compounds 1-3 significantly inhibited the accumulation of the pro-inflammatory iNOS protein in LPS-stimulated RAW264.7 macrophage cells. Compound 3 also showed marked activity in inhibiting the expression of COX-2 protein in the same cells.
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors.forename: Bo-Wei
authors.initials: BW
authors.lastname: Chen
authors.forename: Chih-Hua
authors.initials: CH
authors.lastname: Chao
authors.forename: Jui-Hsin
authors.initials: JH
authors.lastname: Su
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wen
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
authors.forename: Jyh-Horng
authors.initials: JH
authors.lastname: Sheu
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D008070:Lipopolysaccharides; C476503:eunicellin; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2