Serological proteome-oriented screening and application of antigens for the diagnosis of Schistosomiasis japonica.
Schistosomiasis remains a major parasitic disease, with 200 million people infected and 779 million people at risk worldwide. The lack of reliable diagnostic techniques makes this disease difficult to control. In an attempt to discover useful candidates for the diagnosis of schistosomiasis, proteomics in combination with western blotting were employed in this study. This serological proteome assay yielded more than 30 immunodominant spots. Ten of these spots were precisely matched with a homologous two-dimensional electrophoresis (2-DE) gel and successfully identified by LC/MS-MS as corresponding to four different proteins. Of these proteins, SjLAP and SjFBPA were successfully expressed, and their recombinant protein products were further applied in the diagnosis of human Schistosomiasis japonica using ELISA. The ELISA results revealed sensitivities of 98.1% and 87.8% for acute and chronic schistosomiasis with rSjLAP and 100% and 84.7% with rSjFBPA, whereas the assays showed a specificity of 96.7% with both recombinant proteins. After treatment with praziquantel, the titres of the antibodies against both antigens declined significantly (P<0.001). Our data therefore suggest that these antibody-oriented recombinant proteins had a high efficacy for the diagnosis of S. japonica, and 2-DE based screening followed by LC/MS-MS has promising potential in the screening of candidate antigens for the diagnosis of schistosomiasis.
Zhong
Zheng-rong
ZR
The Anhui Provincial Laboratory of Pathogen Biology, Anhui Medical University, Hefei 230032, China.
Zhou
Hua-bang
HB
Li
Xiao-yue
XY
Luo
Qing-li
QL
Song
Xiao-rong
XR
Wang
Wei
W
Wen
Hui-qin
HQ
Yu
Li
L
Wei
Wei
W
Shen
Ji-long
JL
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology; D000909:Antibodies, Helminth; D000947:Antigens, Helminth / Q000276:immunology; D015153:Blotting, Western / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D030561:Databases, Nucleic Acid; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015801:Helminth Proteins / Q000097:blood / Q000276:immunology; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology; D011223:Praziquantel / Q000494:pharmacology; D020543:Proteome / Q000032:analysis / Q000276:immunology; D011817:Rabbits; D012549:Schistosoma japonicum / Q000187:drug effects / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000097:blood / Q000175:diagnosis* / Q000188:drug therapy; D012680:Sensitivity and Specificity; D012908:Snails; D053719:Tandem Mass Spectrometry
D000871:Anthelmintics; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D017931:DNA Primers; D015801:Helminth Proteins; D016056:Immunodominant Epitopes; D020543:Proteome; D011223:Praziquantel