Polo-like kinase 1 is essential to DNA damage recovery.
Radiation treatment and chemotherapy can both cause DNA damage to tumor cells. Tumor cells have specific self-protecting mechanisms to escape death. In this study, we explored the effects of polo-like kinase 1 on the DNA damage recovery system in lymphoma cell lines. DNA damage caused inhibition of polo-like kinase 1 activity and induced cell cycle arrest at the G2/M phase. Once the DNA damage was repaired, polo-like kinase 1 activity was restored and cell cycle arrest was removed. Unexpectedly, polo-like kinase 1 depletion hampered the cell cycle progress of cells recovered from DNA damage, and induced apoptosis.
Liu
Lin
L
Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Zhang
Min
M
Zou
Ping
P
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004249:DNA Damage; D004260:DNA Repair; D004317:Doxorubicin / Q000494:pharmacology; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D018613:Microscopy, Confocal; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D014162:Transfection; D000097869:Polo-Like Kinase 1
D000903:Antibiotics, Antineoplastic; D018797:Cell Cycle Proteins; D004317:Doxorubicin; D049452:Green Fluorescent Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1