Recombinant allergen-based IgE testing to distinguish bee and wasp allergy.
BACKGROUND
The identification of the disease-causing insect in venom allergy is often difficult.


OBJECTIVE
To establish recombinant allergen-based IgE tests to diagnose bee and yellow jacket wasp allergy.


METHODS
Sera from patients with bee and/or wasp allergy (n = 43) and patients with pollen allergy with false-positive IgE serology to venom extracts were tested for IgE reactivity in allergen extract-based tests or with purified allergens, including nonglycosylated Escherichia coli-expressed recombinant (r) Api m 1, rApi m 2, rVes v 5, and insect cell-expressed, glycosylated rApi m 2 as well as 2 natural plant glycoproteins (Phl p 4, bromelain).


RESULTS
The patients with venom allergy could be diagnosed with a combination of E coli-expressed rApi m 1, rApi m 2, and rVes v 5 whereas patients with pollen allergy remained negative. For a group of 29 patients for whom the sensitizing venom could not be identified with natural allergen extracts, testing with nonglycosylated allergens allowed identification of the sensitizing venom. Recombinant nonglycosylated allergens also allowed definition of the sensitizing venom for those 14 patients who had reacted either with bee or wasp venom extracts. By IgE inhibition studies, it is shown that glycosylated Api m 2 contains carbohydrate epitopes that cross-react with natural Api m 1, Ves v 2, natural Phl p 4, and bromelain, thus identifying cross-reactive structures responsible for serologic false-positive test results or double-positivity to bee and wasp extracts.


CONCLUSIONS
Nonglycosylated recombinant bee and wasp venom allergens allow the identification of patients with bee and wasp allergy and should facilitate accurate prescription of venom immunotherapy.
Mittermann
Irene
I
Christian Doppler Laboratory for the Development of Allergen Chips, Medical University of Vienna, Vienna, Austria.
Zidarn
Mihaela
M
Silar
Mira
M
Markovic-Housley
Zora
Z
Aberer
Werner
W
Korosec
Peter
P
Kosnik
Mitja
M
Valenta
Rudolf
R
D000485:Allergens / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000818:Animals; D052179:Antigens, Plant; D001516:Bees; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003937:Diagnosis, Differential; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000150:complications / Q000175:diagnosis* / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008297:Male; D010741:Phospholipases A / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D006255:Rhinitis, Allergic, Seasonal / Q000150:complications / Q000175:diagnosis* / Q000276:immunology; D012699:Serology; D014688:Venoms; D014863:Wasps
D000485:Allergens; D052179:Antigens, Plant; D019476:Insect Proteins; D011994:Recombinant Proteins; D014688:Venoms; C431905:Ves v 5 allergen; D007073:Immunoglobulin E; D010741:Phospholipases A; C497838:Api m 1 allergen, Apis mellifera