title: Comparative quantitation of proteome alterations induced by aging or immortalization in primary human fibroblasts and keratinocytes for clinical applications.
abstract: We monitor proteome changes of primary human skin fibroblasts and keratinocytes during cell culture and compare them to respective immortalized cell lines using stable isotope labeling by amino acids to address their suitability as cell models for clinical disease proteomics.
authors.affiliation: Freiburg Institute for Advanced Studies, School of Life Science-LIFENET, University of Freiburg, Albertstr. 19, 79104 Freiburg, Germany.
authors.forename: Adrian
authors.initials: A
authors.lastname: Sprenger
authors.forename: Victoria
authors.initials: V
authors.lastname: Küttner
authors.forename: Martin L
authors.initials: ML
authors.lastname: Biniossek
authors.forename: Christine
authors.initials: C
authors.lastname: Gretzmeier
authors.forename: Melanie
authors.initials: M
authors.lastname: Boerries
authors.forename: Claudia
authors.initials: C
authors.lastname: Mack
authors.forename: Cristina
authors.initials: C
authors.lastname: Has
authors.forename: Leena
authors.initials: L
authors.lastname: Bruckner-Tuderman
authors.forename: Jörn
authors.initials: J
authors.lastname: Dengjel
mesh_terms: D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*
chemical_list: D020543:Proteome