[Immunological study on decellularized whole laryngeal scaffold].
OBJECTIVE
To evaluate the immunogenicity of the decellularized laryngeal scaffold.


METHODS
Twelve perfused, decellularized laryngeal scaffolds were obtained from rabbits through common carotid artery perfusion with detergents. The twelve decellularized laryngeal scaffolds and the twelve fresh larynxes were then implanted in para-laryngeal muscles of rabbits and harvested after two weeks, four weeks, twelve weeks and twenty-four weeks, respectively. Macroscopic view, histological examination and lymphocyte infiltration test were performed.


RESULTS
The decellularized larynxes were implanted and preserved the laryngeal extracellular matrix and laryngeal architecture. The decellularized larynx did not show obvious immunological rejection after implanted into the para-laryngeal muscles of the recipient rabbits. The volume of implanted larynx became smaller but retained cartilage scaffold. The larynxes in the control group presented the serious immunological rejection and the majority tissues of the larynxes were disintegrated and substituted by the fibrous connective tissues after four weeks. The peripheral tissues were damaged and necrotic at different degrees. The quantity of the lymphocyte infiltration in the control group was higher than that in the experiment group and the result had the statistical significance (P < 0.01).


CONCLUSIONS
Perfused, decellularized technique can construct a low immune laryngeal cartilage scaffold which could be a satisfactory material for laryngeal repair.
Ma
Rui-na
RN
Department of Otorhinolaryngology Head and Neck Surgery, Tangdu Hospital, The Forth Military Medical University, Xi'an 710038, China.
Cui
Peng-cheng
PC
Hou
Nan
N
Luo
Jia-sheng
JS
Wang
Xing
X
D000818:Animals; D002356:Cartilage / Q000166:cytology; D002478:Cells, Cultured; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D007831:Larynx, Artificial; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D019919:Prosthesis Implantation; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds