Resolving myoarchitectural disarray in the mouse ventricular wall with diffusion spectrum magnetic resonance imaging.
The myoarchitecture of the ventricular wall provides a structural template dictating tissue-scale patterns of mechanical function. We studied whether myofiber tract imaging performed with MR diffusion spectrum imaging (DSI) tractography has the capacity to resolve abnormalities of ventricular myoarchitecture in a model of congenital hypertrophic cardiomyopathy (HCM) associated with the ablation of myosin binding protein-C (MyBP-C). Homozygous MyBP-C knockout mice were generated by deletion of exons 3-10 from the endogenous MyBP-C gene. Fiber alignment in the left ventricular wall of wild type mice was depicted through DSI tractography (and confirmed by multi-slice two-photon microscopy) as a set of helical structures whose angles display a continuous transition from negative in the subepicardium to positive in the subendocardium. In contrast, the hearts obtained from the MyBP-C knockouts displayed substantial myoarchitectural disarray, characterized by a loss of voxel-to-voxel orientational coherence for fibers principally located in the mid-myocardium-subendocardium and impairment of the transmural progression of helix angles. These results substantiate the use of DSI tractography in determining myoarchitectural disarray in models of cardiomyopathy and suggest a biological association between myofilament expression, cardiac fiber alignment, and torsional rotation in the setting of congenital HCM.
Wang
Teresa T
TT
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, USA.
Kwon
Hyuk Sang
HS
Dai
Guangping
G
Wang
Ruopeng
R
Mijailovich
Srboljub M
SM
Moss
Richard L
RL
So
Peter T C
PT
Wedeen
Van J
VJ
Gilbert
Richard J
RJ
D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D002312:Cardiomyopathy, Hypertrophic / Q000235:genetics / Q000473:pathology*; D002352:Carrier Proteins / Q000235:genetics; D038524:Diffusion Magnetic Resonance Imaging; D056324:Diffusion Tensor Imaging / Q000379:methods*; D006352:Heart Ventricles / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009206:Myocardium / Q000648:ultrastructure*; D000099163:Myosin Binding Protein C
D002352:Carrier Proteins; D000099163:Myosin Binding Protein C
Circ Cardiovasc Imaging. 2009 May;2(3):206-12
19808594
Circ Res. 2004 May 28;94(10):1279-89
15166115
Neuroimage. 2003 Jul;19(3):482-95
12880782
Circ Res. 2004 Oct 29;95(9):930-6
15472117
NMR Biomed. 2009 Feb;22(2):182-90
18780284
Annu Rev Biomed Eng. 2003;5:147-77
14527312
J Magn Reson Imaging. 2006 Jan;23(1):1-8
16331592
J Mol Biol. 2007 Mar 16;367(1):36-41
17254601
J Mol Biol. 2008 Dec 5;384(1):60-72
18817784
Circ Res. 2002 Mar 22;90(5):594-601
11909824
Appl Opt. 1999 Oct 1;38(28):6004-9
18324120
J Biomed Opt. 2008 Nov-Dec;13(6):064005
19123652
Circ Res. 1981 Oct;49(4):829-42
7023741
J Physiol. 2000 Oct 1;528 Pt 1:151-6
11018113
Circ Res. 1999 May 28;84(10):1117-26
10347086
Magn Reson Imaging. 1996;14(7-8):701-9
8970070
Circ Res. 2006 May 12;98(9):1212-8
16574907
Circ Res. 1969 Mar;24(3):339-47
5766515
Circ Res. 2003 Oct 17;93(8):752-8
14500336
Circ Res. 2007 Oct 26;101(9):928-38
17823372
Circ Res. 1973 Dec;33(6):639-55
4762006
Radiology. 2000 Jul;216(1):128-39
10887238
Biophys J. 2008 Jul;95(2):720-8
18375505
Circ Res. 1995 Jul;77(1):182-93
7788876
Circ Res. 2004 May 14;94(9):1249-55
15059932
Anat Rec A Discov Mol Cell Evol Biol. 2006 Nov;288(11):1173-82
17031810
J Biomed Opt. 2007 Jan-Feb;12(1):014015
17343490
Cell Struct Funct. 1997 Feb;22(1):123-9
9113398
Anat Rec A Discov Mol Cell Evol Biol. 2004 Jan;276(1):43-57
14699633
Dev Biol. 2004 Oct 15;274(2):225-32
15385154
Eur J Cardiothorac Surg. 2005 Feb;27(2):191-201
15691670
Biophys J. 2006 Aug 1;91(3):1014-22
16679361
Am J Physiol. 1981 Jun;240(6):H920-6
7246754
Semin Cell Dev Biol. 2007 Feb;18(1):84-9
17267246
Magn Reson Med. 2003 Jul;50(1):107-13
12815685
Br Heart J. 1981 Mar;45(3):248-63
7008815
Biophys J. 2006 Jun 1;90(11):4119-27
16513777