Untangling metabolic and spatial interactions of stress tolerance in plants. 1. Patterns of carbon metabolism within leaves.
The localization of the key photoreductive and oxidative processes and some stress-protective reactions within leaves of mesophytic C(3) plants were investigated. The role of light in determining the profile of Rubisco, glutamate oxaloacetate transaminase, catalase, fumarase, and cytochrome-c-oxidase across spinach leaves was examined by exposing leaves to illumination on either the adaxial or abaxial leaf surfaces. Oxygen evolution in fresh paradermal leaf sections and CO(2) gas exchange in whole leaves under adaxial or abaxial illumination was also examined. The results showed that the palisade mesophyll is responsible for the midday depression of photosynthesis in spinach leaves. The photosynthetic apparatus was more sensitive to the light environment than the respiratory apparatus. Additionally, examination of the paradermal leaf sections by optical microscopy allowed us to describe two new types of parenchyma in spinach-pirum mesophyll and pillow spongy mesophyll. A hypothesis that oxaloacetate may protect the upper leaf tissue from the destructive influence of active oxygen is presented. The application of mathematical modeling shows that the pattern of enzymatic distribution across leaves abides by the principle of maximal ecological utility. Light regulation of carbon metabolism across leaves is discussed.
Biel
Karl Y
KY
Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. karlbiel@hotmail.com
Fomina
Irina R
IR
Nazarova
Galina N
GN
Soukhovolsky
Vladislav G
VG
Khlebopros
Rem G
RG
Nishio
John N
JN
D024443:Aspartate Aminotransferase, Cytoplasmic / Q000378:metabolism; D001639:Bicarbonates / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D008027:Light; D008291:Malate Dehydrogenase / Q000378:metabolism; D008962:Models, Theoretical; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology; D018515:Plant Leaves / Q000033:anatomy & histology / Q000201:enzymology*; D010940:Plant Proteins / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D018724:Spinacia oleracea / Q000033:anatomy & histology / Q000378:metabolism; D013312:Stress, Physiological
D001639:Bicarbonates; D010940:Plant Proteins; D002734:Chlorophyll; D002245:Carbon Dioxide; D002244:Carbon; D008291:Malate Dehydrogenase; D002374:Catalase; D024443:Aspartate Aminotransferase, Cytoplasmic; D012273:Ribulose-Bisphosphate Carboxylase; D010100:Oxygen
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