title: Prevention of Candida albicans biofilm formation by covalently bound dimethylaminoethylmethacrylate and polyethylenimine.
abstract: Candida albicans biofilms are a major cause of voice prosthesis deterioration in laryngectomized patients. The aim of this study was to produce a surface capable of inhibiting C. albicans biofilm formation. Dimethylaminoethylmethacrylate (DMAEMA) and polyethylenimine (PEI) moieties were covalently bound to the surface of polydimethylsiloxane (PDMS) or polymethylmethacrylate (PMMA) and subsequently quaternized. Physicochemical characterization of the grafted surfaces was carried out and their effect on C. albicans cell numbers was assessed using a modified Robbins device to grow the biofilms. Covalently bound quaternized polyDMAEMA (polyDMAEMAq) and PEI (PEIq) inhibited biofilm growth, with reductions up to 92%. Our approach may show promise for future application in medical devices such as catheters and prostheses.
authors.affiliation: Laboratory of Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium.
authors.forename: Kristof
authors.initials: K
authors.lastname: De Prijck
authors.forename: Nele
authors.initials: N
authors.lastname: De Smet
authors.forename: Tom
authors.initials: T
authors.lastname: Coenye
authors.forename: Etienne
authors.initials: E
authors.lastname: Schacht
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Nelis
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development*; D015169:Colony Count, Microbial / Q000379:methods; D004129:Dimethylpolysiloxanes / Q000378:metabolism; D004864:Equipment and Supplies / Q000382:microbiology; D005021:Ethylamines / Q000494:pharmacology*; D006801:Humans; D008689:Methacrylates / Q000494:pharmacology*; D011094:Polyethyleneimine / Q000494:pharmacology*; D019904:Polymethyl Methacrylate / Q000378:metabolism
chemical_list: C510335:2-dimethylaminoethyl methacrylate; D000935:Antifungal Agents; D004129:Dimethylpolysiloxanes; D005021:Ethylamines; D008689:Methacrylates; C013830:baysilon; D011094:Polyethyleneimine; D019904:Polymethyl Methacrylate
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