A method for DNA and RNA co-extraction for use on forensic samples using the Promega DNA IQ™ system.
The use of messenger RNA profiling to identify the origin of biological samples (e.g. blood, semen and saliva) from crime scenes is now at the stage of being implemented into routine forensic casework. We report on the successful modification of the Promega DNA IQ™ system to enable co-extraction of DNA and RNA from the same sample without compromising the potential DNA profile. Using the protocol in our laboratory for extracting DNA using the DNA IQ™ system combined with the Zymo Research Mini RNA Isolation Kit™ II we demonstrate the simultaneous co-extraction of DNA and RNA from the same sample for routine DNA and mRNA profiling for the identification of both the individual and the biological stain.
Bowden
Anna
A
Department of Chemistry, University of Auckland, New Zealand.
Fleming
Rachel
R
Harbison
SallyAnn
S
D001774:Blood Chemical Analysis / Q000379:methods; D001801:Blood Stains; D003415:Crime; D004247:DNA / Q000032:analysis* / Q000097:blood / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D017408:Guidelines as Topic; D006801:Humans; D008297:Male; D012313:RNA / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D012463:Saliva / Q000737:chemistry; D012661:Semen / Q000737:chemistry
C034477:RNA primers; D012333:RNA, Messenger; D011933:Reagent Kits, Diagnostic; D012313:RNA; D004247:DNA