Isolation and characterization of enantioselective DNA aptamers for ibuprofen.
Single stranded DNA aptamers that can bind to ibuprofen, a widely used anti-inflammation drug, were selected from random DNA library of 10(15) nucleotides by FluMag-SELEX process. Five different sequences were selected and their enantioselectivity and affinity were characterized. Three out of five aptamer candidates did not show any affinity to (S)-ibuprofen, but only to racemic form of ibuprofen, suggesting that they are (R)-ibuprofen specific aptamers. Another two aptamer candidates showed affinity to both racemic form and (S)-ibuprofen, which were considered as (S)-ibuprofen specific aptamers. The affinity of five ssDNA aptamers isolated was in a range of 1.5-5.2microM. In addition, all of these five aptamers did not show any affinity to analogues of ibuprofen in its profen's group (fenoprofen, flubiprofen, and naproxen) and the antibiotics of oxytetracycline, another control.
Kim
Yeon Seok
YS
School of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-Gu, Seoul 136-701, South Korea.
Hyun
Chang Jun
CJ
Kim
In Ae
IA
Gu
Man Bock
MB
D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000302:isolation & purification*; D001483:Base Sequence / Q000187:drug effects; D001665:Binding Sites; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded / Q000378:metabolism*; D015723:Gene Library; D007052:Ibuprofen / Q000737:chemistry* / Q000494:pharmacology; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D009841:Oligonucleotides / Q000737:chemistry*; D052156:SELEX Aptamer Technique; D013329:Structure-Activity Relationship
D052157:Aptamers, Nucleotide; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D007052:Ibuprofen