[Expression and clinical significance of calcium-activated chloride channel and mucins in nasal mucosa with allergic rhinitis].
OBJECTIVE
To evaluate the role of human calcium-activated chloride channel 1 (hCLCA1) and mucin MUC5AC in allergic rhinitis.


METHODS
The expression of hCLCA1 and MUC5AC were determined by RT-PCR and immunohistochemical assay in nasal mucosa on 20 patients with allergic rhinitis and 7 normal persons.


RESULTS
The expression of hCLCA1mRNA in allergic rhinitis group was positive, whereas in normal control group was absent (P<0.01). The expression of MUC5AC mRNA and MUC5AC protein in the allergic rhinitis group were higher than that in the control, respectively (both P<0.01). The increased expression of hCLCA1mRNA in allergic rhinitis group were well correlated with the expression of MUC5ACmRNA and MUC5AC protein and the correlation coefficients were 0.752 and 0.694(both P<0.05).


CONCLUSIONS
Upregulation of hCLCA1 mRNA expression may play a pivotal role in mucus overproduction in allergic rhinitis group. The inhibition of HCLCA1 expression may provide a new strategy for the treatment of allergic rhinitis.
Wu
Wenli
W
Department Otorhinolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Zhen
Hongtao
H
Cui
Yonghua
Y
Chu
Hanqi
H
Wang
Lifen
L
D000293:Adolescent; D000328:Adult; D018118:Chloride Channels / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC / Q000378:metabolism*; D009297:Nasal Mucosa / Q000378:metabolism*; D012221:Rhinitis, Allergic, Perennial / Q000378:metabolism*; D055815:Young Adult
C492109:CLCA1 protein, human; D018118:Chloride Channels; C524678:MUC5AC protein, human; D055271:Mucin 5AC