title: Experimental model of myocardial infarction: Histopathology and reperfusion damage revisited.
abstract: The goal of this pilot study was to create an experimental model of myocardial infarction (for subsequent evaluation of the effectiveness of an alternative way of stem cell application - intracoronary cell infusion in the management of acute myocardial infarction). Four experimental animals, female pigs weighing between 30 and 40 kg, were used in the initial phase of this study to create an experimental model of acute myocardial infarction. An experimental myocardial infarction was performed via occlusion of the interventricular arm of the left coronary artery for 90 min. The hearts were examined 1 h, 3 days, 5 days, and 7 days after the procedure. Macroscopically, red infarction characteristic of reperfusion was found. Microscopically, the healing process with granulation tissue production/collagen deposition was remarkably accelerated compared to literature data. Repair processes in reperfused experimental myocardial infarction and/or reperfused autopsy specimens should not be evaluated on the basis of literature data only. Large collections of extracellular calcium were present. This phenomenon is not well described in the literature and probably has the potential for significantly interfering with the repair process. The histopathology of reperfused acute myoardial infarction deserves to be studied in further investigations.
authors.affiliation: Department of Pathology, University Hospital Brno, Brno 625 00, Czech Republic. lkren@fnbrno.cz
authors.forename: Leos
authors.initials: L
authors.lastname: Kren
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Meluzin
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Pavlovsky
authors.forename: Jiri
authors.initials: J
authors.lastname: Mayer
authors.forename: Petr
authors.initials: P
authors.lastname: Kala
authors.forename: Ladislav
authors.initials: L
authors.lastname: Groch
authors.forename: Ivan
authors.initials: I
authors.lastname: Hornacek
authors.forename: Petr
authors.initials: P
authors.lastname: Rauser
authors.forename: Michal
authors.initials: M
authors.lastname: Vlasin
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006097:Granulation Tissue / Q000473:pathology*; D002479:Inclusion Bodies / Q000378:metabolism; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D010865:Pilot Projects; D033581:Stem Cell Transplantation; D013552:Swine
chemical_list: D002118:Calcium