[Effect of formaldehyde on expressions of Fas apoptosis pathway-related genes of ovary tissues in female rats].
OBJECTIVE
To explore the mechanism of formaldehyde inducing ovarian toxicity in female rats by observing the effect of formaldehyde on the expression of Fas and caspase-8 mRNA, and the activity of caspase-3 and caspase-8 of ovary tissues in female rats.


METHODS
Forty female Sprague-Dawley(SD) rats were randomly divided into a control group and 3 formaldehyde groups at different concentrations. The rats in the formaldehyde groups were intraperitoneally injected different doses of formaldehyde (20.0,2.0 and 0.2 mg/kg) continuously for 14 days.After 14 days, all rats were sacrificed and their ovaries were collected for detecting the expression of Fas and caspase-8 mRNA with RT-PCR, the protein expression of Fas with Western blot, and the activities of caspase-8 and caspase-3 with spectrophotometric method.


RESULTS
Compared with the control group, the expression of Fas mRNA and its protein and caspase-8 mRNA and the activity of caspase-8 and caspase-3 of ovary tissues in the rats treated with formaldehyde significantly increased with dose (P<0.05).


CONCLUSIONS
The increase of Fas gene expression and the activity of caspase-8 and caspase-3 may be the important mechanism of ovarian toxicity induced by formaldehyde in female rats.
Peng
Guoqing
G
Department of Toxicology, School of Public Health, Central South University, Changsha 410078, China.
Zhong
Caigao
C
Zhang
Qiong
Q
Xie
Ying
Y
Gong
Fengying
F
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053181:Caspase 8 / Q000235:genetics / Q000378:metabolism; D004785:Environmental Pollutants / Q000633:toxicity; D005260:Female; D005557:Formaldehyde / Q000633:toxicity*; D010053:Ovary / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
D004785:Environmental Pollutants; C491898:Fas protein, rat; D012333:RNA, Messenger; D019014:fas Receptor; D005557:Formaldehyde; C505410:Casp3 protein, rat; C505528:Casp8 protein, rat; D053148:Caspase 3; D053181:Caspase 8