title: [Expression and clinical significance of calcium-activated chloride channel and mucins in nasal mucosa with allergic rhinitis].
abstract: OBJECTIVE
  To evaluate the role of human calcium-activated chloride channel 1 (hCLCA1) and mucin MUC5AC in allergic rhinitis.
  
  
  METHODS
  The expression of hCLCA1 and MUC5AC were determined by RT-PCR and immunohistochemical assay in nasal mucosa on 20 patients with allergic rhinitis and 7 normal persons.
  
  
  RESULTS
  The expression of hCLCA1mRNA in allergic rhinitis group was positive, whereas in normal control group was absent (P<0.01). The expression of MUC5AC mRNA and MUC5AC protein in the allergic rhinitis group were higher than that in the control, respectively (both P<0.01). The increased expression of hCLCA1mRNA in allergic rhinitis group were well correlated with the expression of MUC5ACmRNA and MUC5AC protein and the correlation coefficients were 0.752 and 0.694(both P<0.05).
  
  
  CONCLUSIONS
  Upregulation of hCLCA1 mRNA expression may play a pivotal role in mucus overproduction in allergic rhinitis group. The inhibition of HCLCA1 expression may provide a new strategy for the treatment of allergic rhinitis.
authors.affiliation: Department Otorhinolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Wu
authors.forename: Hongtao
authors.initials: H
authors.lastname: Zhen
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Cui
authors.forename: Hanqi
authors.initials: H
authors.lastname: Chu
authors.forename: Lifen
authors.initials: L
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D018118:Chloride Channels / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC / Q000378:metabolism*; D009297:Nasal Mucosa / Q000378:metabolism*; D012221:Rhinitis, Allergic, Perennial / Q000378:metabolism*; D055815:Young Adult
chemical_list: C492109:CLCA1 protein, human; D018118:Chloride Channels; C524678:MUC5AC protein, human; D055271:Mucin 5AC