title: Establishment of L-arabinose fermentation in glucose/xylose co-fermenting recombinant Saccharomyces cerevisiae 424A(LNH-ST) by genetic engineering.
abstract: Cost-effective and efficient ethanol production from lignocellulosic materials requires the fermentation of all sugars recovered from such materials including glucose, xylose, mannose, galactose, and L-arabinose. Wild-type strains of Saccharomyces cerevisiae used in industrial ethanol production cannot ferment D-xylose and L-arabinose. Our genetically engineered recombinant S. cerevisiae yeast 424A(LNH-ST) has been made able to efficiently ferment xylose to ethanol, which was achieved by integrating multiple copies of three xylose-metabolizing genes. This study reports the efficient anaerobic fermentation of L-arabinose by the derivative of 424A(LNH-ST). The new strain was constructed by over-expression of two additional genes from fungi L-arabinose utilization pathways. The resulting new 424A(LNH-ST) strain exhibited production of ethanol from L-arabinose, and the yield was more than 40%. An efficient ethanol production, about 72.5% yield from five-sugar mixtures containing glucose, galactose, mannose, xylose, and arabinose was also achieved. This co-fermentation of five-sugar mixture is important and crucial for application in industrial economical ethanol production using lignocellulosic biomass as the feedstock.
authors.affiliation: Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN, USA.
authors.forename: Aloke Kumar
authors.initials: AK
authors.lastname: Bera
authors.forename: Miroslav
authors.initials: M
authors.lastname: Sedlak
authors.forename: Aftab
authors.initials: A
authors.lastname: Khan
authors.forename: Nancy W Y
authors.initials: NW
authors.lastname: Ho
mesh_terms: D001089:Arabinose / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D014994:Xylose; D001089:Arabinose; D005947:Glucose