Disruption of the putative cell surface polysaccharide biosynthesis gene SO3177 in Shewanella oneidensis MR-1 enhances adhesion to electrodes and current generation in microbial fuel cells.
A microbial fuel cell (MFC) was inoculated with a random transposon insertion mutant library of Shewanella oneidensis MR-1 and operated with lactate as the sole fuel to select for mutants that preferentially grew in it. Agar plate cultivation of the resultant MFC enrichment culture detected an increased number of colonies exhibiting rough morphology. One such isolate, strain 4A, generated 50% more current in an MFC than wild-type MR-1. Determination of the transposon insertion site in strain 4A followed by deletion and complementation experiments revealed that the SO3177 gene, encoding a putative formyltransferase and situated in a cell surface polysaccharide biosynthesis gene cluster, was responsible for the increased current. Transmission electron microscopy showed that a layered structure at the cell surface, stainable with ruthenium red, was impaired in the SO3177 mutant (DeltaSO3177), confirming that SO3177 is involved in the biosynthesis of cell surface polysaccharides. Compared to the wild type, DeltaSO3177 cells preferentially attached to graphite felt anodes in MFCs, while physicochemical analyses revealed that the cell surface of DeltaSO3177 was more hydrophobic. These results demonstrate that cell surface polysaccharides affect not only the cell adhesion to graphite anodes but also the current generation in MFCs.
Kouzuma
Atsushi
A
Hashimoto Light Energy Conversion Project, ERATO/JST, Komaba Open Laboratory, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Meng
Xian-Ying
XY
Kimura
Nobutada
N
Hashimoto
Kazuhito
K
Watanabe
Kazuya
K
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001674:Bioelectric Energy Sources / Q000382:microbiology; D002473:Cell Wall / Q000737:chemistry; D004251:DNA Transposable Elements / Q000235:genetics; D004560:Electricity; D004566:Electrodes / Q000382:microbiology*; D017353:Gene Deletion; D019877:Hydroxymethyl and Formyl Transferases / Q000235:genetics* / Q000378:metabolism; D016254:Mutagenesis, Insertional; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D020592:Shewanella / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000502:physiology
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D011135:Polysaccharides, Bacterial; D019877:Hydroxymethyl and Formyl Transferases
Microbiology (Reading). 2001 May;147(Pt 5):1365-1382
11320139
Appl Environ Microbiol. 2004 Feb;70(2):1234-7
14766612
FEMS Microbiol Lett. 2003 Jun 6;223(1):129-34
12799011
Science. 1988 Jun 3;240(4857):1319-21
17815852
Appl Environ Microbiol. 1999 Dec;65(12):5328-33
10583984
Nat Biotechnol. 2003 Oct;21(10):1229-32
12960964
J Clin Microbiol. 1990 Dec;28(12):2627-31
1704012
Curr Opin Microbiol. 2002 Jun;5(3):296-300
12057684
Gene. 1995 Dec 1;166(1):175-6
8529885
J Biol Chem. 2005 Apr 8;280(14):14154-67
15695810
J Bacteriol. 1983 Apr;154(1):269-77
6187729
Environ Sci Technol. 2006 Apr 1;40(7):2426-32
16646485
FEMS Microbiol Lett. 2007 Jul;272(2):127-36
17521360
Appl Environ Microbiol. 2003 Mar;69(3):1548-55
12620842
Appl Environ Microbiol. 2002 Sep;68(9):4653-7
12200327
Int J Food Microbiol. 2004 Jun 15;93(3):281-6
15163584
J Biosci Bioeng. 2008 Dec;106(6):528-36
19134546
Nature. 2000 May 4;405(6782):94-7
10811225
Microbiology (Reading). 2007 Jun;153(Pt 6):1872-1883
17526844
Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11358-63
16849424
J Ind Microbiol. 1995 Sep;15(3):162-8
8519473
Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10983-8
12939408
Nat Biotechnol. 2002 Nov;20(11):1118-23
12368813
J Bacteriol. 2006 Aug;188(15):5510-23
16855241
J Bacteriol. 2010 Jan;192(2):467-74
19897659
J Bacteriol. 2001 Jun;183(12):3811-5
11371548
Gene. 1995 Jul 4;160(1):59-62
7628717
Adv Microb Physiol. 2004;49:219-86
15518832
Appl Environ Microbiol. 1997 Oct;63(10):3837-43
16535706
Environ Sci Technol. 2006 Sep 1;40(17):5181-92
16999087
J Am Chem Soc. 2006 Nov 1;128(43):13978-9
17061851
Curr Opin Biotechnol. 2009 Dec;20(6):633-41
19833503
Annu Rev Microbiol. 2007;61:401-22
17506679
Appl Environ Microbiol. 2008 Feb;74(3):615-23
18065612
Carbohydr Res. 2003 Sep 10;338(19):1991-7
14499575
Environ Sci Technol. 2010 Jan 1;44(1):68-73
20039735
Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3968-73
18316736
Appl Environ Microbiol. 1994 Feb;60(2):740-5
16349202
Appl Environ Microbiol. 2009 Dec;75(24):7674-81
19837834
Appl Environ Microbiol. 2007 Nov;73(21):7003-12
17644630