Interlaboratory validation of an event-specific real time polymerase chain reaction detection method for genetically modified DAS59132 maize.
A real-time polymerase chain reaction (PCR) method specific for genetically modified (GM) maize event DAS59132 (E32) was adapted for qualitative detection of low level presence of E32. The method was validated by a collaborative trial with eight participating Japanese laboratories. Sensitivity was assessed with three different samples of corn flour fortified to 0%, 0.05% and 0.1% (w/w) E32 respectively. In addition, a 0.01% E32 DNA solution was used. The detection limit with DNA solution was estimated to be approximately 0.01%. In conclusion, the results of the study confirmed this real-time PCR method as a reliable tool for qualitative detection of E32 maize.
Akiyama
Hiroshi
H
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Sakata
Kozue
K
Spiegelhalter
Frank
F
Furui
Satoshi
S
Nakashima
Akie
A
Kitta
Kazumi
K
Teshima
Reiko
R
D018744:DNA, Plant; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D030841:Food, Genetically Modified; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions; D003313:Zea mays / Q000235:genetics
D018744:DNA, Plant; D012996:Solutions