title: Successful multiple pregnancy achieved after transfer of frozen embryos obtained via intracytoplasmic sperm injection with testicular sperm from an AZFc-deleted man.
abstract: OBJECTIVE
  To describe a case of successful triplet pregnancy after testicular sperm extraction (TESE) from a man with AZFc deletion and intracytoplasmic sperm injection (ICSI).
  
  
  METHODS
  Case report.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  A 38-year-old man affected by complete AZFc deletion and azoospermia.
  
  
  METHODS
  Spermiogram, Y-chromosome microdeletion screening, TESE for sperm recovery from testicular tissue on the same day as ICSI, transfer of frozen-thawed embryos, vaginal ultrasound examination.
  
  
  METHODS
  The Y chromosome genetic status of an azoospermic patient who underwent TESE and ICSI, the fertilization and pregnancy outcome.
  
  
  RESULTS
  The patient was found to be azoospermic, and the deletion screening showed complete AZFc deletion. After TESE, the recovered testicular sperm were selected for ICSI. Three good quality embryos were obtained and were frozen due to ovarian hyperstimulation syndrome in the female partner. After transfer of the thawed embryos, a triplet pregnancy was diagnosed by vaginal ultrasonography at the seventh week of gestation. Two male and one female healthy babies were born.
  
  
  CONCLUSIONS
  This is the first report of a successful triplet pregnancy after the transfer of frozen-thawed embryos in a couple in whom the male partner was azoospermic and a carrier of complete AZFc deletion. This deletion should not adversely affect a man's TESE retrieval prognosis or the fertilization, cleavage, and implantation of embryos. The offspring were healthy, although the two sons inherited the AZFc deletion.
authors.affiliation: Center for the Diagnosis and Treatment of Couple Sterility, Siena Hospital, University of Siena, Siena, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Gambera
authors.forename: Laura
authors.initials: L
authors.lastname: Governini
authors.forename: Vincenzo
authors.initials: V
authors.lastname: De Leo
authors.forename: Alice
authors.initials: A
authors.lastname: Luddi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Morgante
authors.forename: Valeria
authors.initials: V
authors.lastname: Tallis
authors.forename: Paola
authors.initials: P
authors.lastname: Piomboni
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D001755:Blastocyst; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005615:Freezing; D017353:Gene Deletion; D056426:Genetic Loci; D006801:Humans; D008297:Male; D011247:Pregnancy; D011272:Pregnancy, Multiple; D029589:Seminal Plasma Proteins / Q000235:genetics*; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology; D016896:Treatment Outcome; D014310:Triplets
chemical_list: D029589:Seminal Plasma Proteins