title: The nanoscale properties of bacterial inclusion bodies and their effect on mammalian cell proliferation.
abstract: The chemical and mechanical properties of bacterial inclusion bodies, produced in different Escherichia coli genetic backgrounds, have been characterized at the nanoscale level. In regard to wild type, DnaK(-) and ClpA(-) strains produce inclusion bodies with distinguishable wettability, stiffness and stiffness distribution within the proteinaceous particle. Furthermore it was possible to observe how cultured mammalian cells respond differentially to inclusion body variants when used as particulate materials to engineer the nanoscale topography, proving that the actual range of referred mechanical properties is sensed and discriminated by biological systems. The data provide evidence of the mechanistic activity of the cellular quality control network and the regulation of the stereospecific packaging of partially folded protein species in bacteria. This inclusion body nanoscale profiling offers possibilities for their fine genetic tuning and the resulting macroscopic effects when applied in biological interfaces.
authors.affiliation: Departament de Nanociència Molecular i Materials Orgànics. Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra, 08193 Barcelona, Spain.
authors.forename: César
authors.initials: C
authors.lastname: Díez-Gil
authors.forename: Sven
authors.initials: S
authors.lastname: Krabbenborg
authors.forename: Elena
authors.initials: E
authors.lastname: García-Fruitós
authors.forename: Esther
authors.initials: E
authors.lastname: Vazquez
authors.forename: Escarlata
authors.initials: E
authors.lastname: Rodríguez-Carmona
authors.forename: Imma
authors.initials: I
authors.lastname: Ratera
authors.forename: Nora
authors.initials: N
authors.lastname: Ventosa
authors.forename: Joaquin
authors.initials: J
authors.lastname: Seras-Franzoso
authors.forename: Olivia
authors.initials: O
authors.lastname: Cano-Garrido
authors.forename: Neus
authors.initials: N
authors.lastname: Ferrer-Miralles
authors.forename: Antonio
authors.initials: A
authors.lastname: Villaverde
authors.forename: Jaume
authors.initials: J
authors.lastname: Veciana
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006224:Cricetinae; D004926:Escherichia coli / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D002479:Inclusion Bodies / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D017155:Wettability
chemical_list: D001672:Biocompatible Materials