[Role of the surgical pathology laboratory in the pre-analytical approach of molecular biology techniques].
The advent of the targeted cancer therapies administered to patients, according to the results of molecular biology techniques (in particular, in situ hybridization, "polymerase chain reaction" amplification and sequencing), has modified the practice of the surgical pathology laboratories. The necessity to answer to the needs of physicians for optimizing the medical care for patients who develop cancer has led to a policy of national debate, spurred by the National Institute of Cancer (INCa), in order to implement new procedures in the pathology laboratories. Thus, in addition to the structuring of molecular biology platforms and their labeling by INCa, the upstream control of the steps present between resection of tumor samples and molecular analysis has proved to be crucial. Indeed, the quality of this upstream time, called "pre-analytical" phase, determines the reliability of the molecular biology results and therefore the therapeutic strategy. We describe here the main steps to be checked in the pre-analytical phase. The optimization of this pre-analytical phase within the surgical pathology laboratory aims to reduce or render insignificant the risk of errors of molecular biology tests. These errors can indeed lead to false negative or false positive results whose therapeutic consequences can be particularly harmful to patients with cancer.
Hofman
Véronique
V
Laboratoire de pathologie clinique et expérimentale, hôpital Pasteur, CHU de Nice, 30, avenue de Voie-Romaine, BP 69, 06002 Nice cedex, France. hofman.p@chu-nice.fr
Ilie
Marius
M
Gavric-Tanga
Virginie
V
Lespinet
Virgine
V
Mari
Mireille
M
Lassalle
Sandra
S
Butori
Catherine
C
Coelle
Céline
C
Bordone
Olivier
O
Selva
Eric
E
Lamy
Aude
A
Sabourin
Jean-Christophe
JC
Hofman
Paul
P
D016477:Artifacts; D018070:Biological Specimen Banks; D014408:Biomarkers, Tumor / Q000032:analysis; D035843:Biomedical Research / Q000379:methods; D002458:Cell Fractionation / Q000379:methods; D015510:Clinical Medicine / Q000379:methods; D015925:Cryopreservation / Q000379:methods; D003951:Diagnostic Errors / Q000517:prevention & control; D005260:Female; D005404:Fixatives / Q000494:pharmacology / Q000633:toxicity; D005557:Formaldehyde / Q000494:pharmacology / Q000633:toxicity; D016591:Histocytological Preparation Techniques / Q000379:methods* / Q000592:standards; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000473:pathology*; D009696:Nucleic Acids / Q000302:isolation & purification; D010338:Pathology, Clinical / Q000379:methods* / Q000592:standards; D057089:Pathology, Molecular / Q000379:methods* / Q000592:standards; D011309:Preservation, Biological / Q000379:methods; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods* / Q000592:standards; D016707:Tissue Fixation / Q000379:methods
D014408:Biomarkers, Tumor; D005404:Fixatives; D009696:Nucleic Acids; D005557:Formaldehyde