title: Clock gene expression in gravid uterus and extra-embryonic tissues during late gestation in the mouse.
abstract: Evidence in humans and rodents suggests the importance of circadian rhythmicity in parturition. A molecular clock underlies the generation of circadian rhythmicity. While this molecular clock has been identified in numerous tissues, the expression and regulation of clock genes in tissues relevant to parturition is largely undefined. Here, the expression and regulation of the clock genes Bmal1, Clock, cryptochrome (Cry1/2) and period (Per1/2) was examined in the murine gravid uterus, placenta and fetal membranes during late gestation. All clock genes examined were expressed in the tissues of interest throughout the last third of gestation. Upregulation of a subset of these clock genes was observed in each of these tissues in the final two days of gestation. Oscillating expression of mRNA for a subset of the examined clock genes was detected in the gravid uterus, placenta and fetal membranes. Furthermore, bioluminescence recording on explants from gravid Per2::luciferase mice indicated rhythmic expression of PER2 protein in these tissues. These data demonstrate expression and rhythmicity of clock genes in tissues relevant to parturition indicating a potential contribution of peripheral molecular clocks to this process.
authors.affiliation: Molecular Cell Biology Program, Washington University, St Louis, MO 63110, USA.
authors.forename: Christine K
authors.initials: CK
authors.lastname: Ratajczak
authors.forename: Erik D
authors.initials: ED
authors.lastname: Herzog
authors.forename: Louis J
authors.initials: LJ
authors.lastname: Muglia
mesh_terms: D056930:ARNTL Transcription Factors / Q000235:genetics; D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics; D002940:Circadian Rhythm / Q000235:genetics*; D056925:Circadian Rhythm Signaling Peptides and Proteins / Q000235:genetics*; D056931:Cryptochromes / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D005865:Gestational Age; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D036801:Parturition / Q000235:genetics / Q000502:physiology*; D056950:Period Circadian Proteins / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D014599:Uterus / Q000737:chemistry*
chemical_list: D056930:ARNTL Transcription Factors; C538961:Bmal1 protein, mouse; D056925:Circadian Rhythm Signaling Peptides and Proteins; C538964:Cry1 protein, mouse; C538967:Cry2 protein, mouse; D056931:Cryptochromes; C539023:Per1 protein, mouse; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D056926:CLOCK Proteins; C538944:Clock protein, mouse
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