Vitamin D receptor gene polymorphism and bone mineral density in 0-6-year-old Han children.
This study aims to investigate the four vitamin D receptor (VDR) gene single nucleotide polymorphisms and their possible relationship with bone mineral density (BMD) in Chinese 0-6-year-old Han children. Two hundred four 0-6-year-old Han children without metabolic bone disease were randomly recruited in Shanghai, China. The BMD of the middle tibia was measured by an ultrasonic bone density instrument. VDR genotypes were determined by polymerase chain reaction restriction fragment length polymorphism using endonuclease ApaI, BsmI, TaqI and FokI. The alleles of a, T, b and F and the genotypes of aa, TT, bb and Ff were predominant. The frequency alleles of a, T, b and F were, respectively, 70.6, 95.8, 95.3 and 57.6%. When the influences of confounders such as serum 25(OH)D, serum zinc and outdoor activities on BMD were removed, the genotypes of BsmI and FokI were found apparently to be related to BMD. The BMD of the Bb carrier was much lower than that of the bb carrier (22.00 ± 27.84 and 43.14 ± 31.98, P < 0.05). The BMD of the ff carrier was lower than that of the Ff or FF carrier (26.97 ± 34.22 and 37.95 ± 29.70 and 53.52 ± 30.35, P < 0.001), while the genotypes of ApaI and TaqI have no relation with BMD in 0-6-year-old Han children. These findings show that the Bb and ff genotypes of the VDR BsmI and FokI variants are significantly associated with a decreased BMD in Chinese Han children aged 0-6 years, while the VDR ApaI and TaqI polymorphisms are not significantly associated with it.
Yu
Xiao-Dan
XD
Shanghai Institute of Pediatric Research, Xinhua Hospital, Medical School of Shanghai Jiaotong University, Shanghai, China.
Shen
Xiao-Ming
XM
Xue
Ming-Bao
MB
Yan
Chong-Huai
CH
D015519:Bone Density / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D018167:Receptors, Calcitriol / Q000235:genetics*
D018167:Receptors, Calcitriol
Dig Dis Sci. 2009 Mar;54(3):634-9
18618252
Clin Chim Acta. 2007 Sep;384(1-2):155-9
17582391
Eur J Clin Invest. 2000 Jul;30(7):608-17
10886301
Pediatr Res. 2001 May;49(5):713-8
11328957
J Bone Miner Res. 2007 Aug;22(8):1313; author reply 1314
17402848
J Bone Miner Res. 2005 Jun;20(6):945-53
15883634
J Bone Miner Res. 1995 Jun;10(6):991-6
7572325
N Engl J Med. 1997 Jul 10;337(2):77-82
9211676
Nature. 1994 Jan 20;367(6460):284-7
8161378
J Bone Miner Res. 2000 Nov;15(11):2206-10
11092401
Epidemiol Rev. 2000;22(2):203-17
11218372
Clin Rheumatol. 2009 Oct;28(10):1187-91
19609483
Pediatr Res. 1998 Nov;44(5):628-32
9803441
J Bone Miner Res. 1998 Nov;13(11):1691-9
9797477
J Bone Miner Res. 2009 Jul;24(7):1319-25
19257820
Invest Clin. 2008 Mar;49(1):29-38
18524329
J Bone Miner Res. 1999 May;14(5):740-6
10320522
J Bone Miner Metab. 2004;22(5):479-85
15316869
Chin Med J (Engl). 2002 Feb;115(2):254-7
11940343
J Bone Miner Metab. 2003;21(2):109-13
12601576
J Periodontol. 1999 Sep;70(9):1032-8
10505806
Annu Rev Nutr. 1998;18:233-58
9706225
Eur J Appl Physiol. 2010 Jan;108(1):31-8
19756704
J Nutr. 2008 Feb;138(2):277-81
18203891
Calcif Tissue Int. 2007 Jan;80(1):15-20
17164973
Exp Physiol. 2005 Jul;90(4):653-61
15890796
J Bone Miner Res. 1997 Jun;12(6):915-21
9169350
Clin Endocrinol (Oxf). 2006 Jun;64(6):625-31
16712663
Clin Chim Acta. 2008 Sep;395(1-2):111-4
18570891
Clin Chem. 1993 Mar;39(3):529-33
8448871
Endocr Relat Cancer. 2008 Dec;15(4):975-83
18719092
J Bone Miner Metab. 2001;19(5):302-7
11498732
J Bone Miner Metab. 2006;24(4):329-36
16816928
Mcgill J Med. 2008 Jul;11(2):132-40
19148311
Eur J Radiol. 2010 Nov;76(2):265-8
19545958
Environ Health Prev Med. 2009 Nov;14(6):328-35
19705233
J Bone Miner Res. 1998 Jun;13(6):925-30
9626623
J Appl Physiol (1985). 2002 Dec;93(6):1973-9
12391072