title: Population structure and abundance of arsenite-oxidizing bacteria along an arsenic pollution gradient in waters of the upper isle River Basin, France.
abstract: Denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR (qPCR) were successfully developed to monitor functional aoxB genes as markers of aerobic arsenite oxidizers. DGGE profiles showed a shift in the structure of the aoxB-carrying bacterial population, composed of members of the Alpha-, Beta- and Gammaproteobacteria, depending on arsenic (As) and E(h) levels in Upper Isle River Basin waters. The highest aoxB gene densities were found in the most As-polluted oxic surface waters but without any significant correlation with environmental factors. Arsenite oxidizers seem to play a key role in As mobility in As-impacted waters.
authors.affiliation: BRGM, Ecotechnology Unit, Process and Environment Division, 3 Avenue Claude Guillemin, 45060 Orléans Cedex 02, France. c.joulian@brgm.fr.
authors.forename: Marianne
authors.initials: M
authors.lastname: Quéméneur
authors.forename: Aurélie
authors.initials: A
authors.lastname: Cébron
authors.forename: Patrick
authors.initials: P
authors.lastname: Billard
authors.forename: Fabienne
authors.initials: F
authors.lastname: Battaglia-Brunet
authors.forename: Francis
authors.initials: F
authors.lastname: Garrido
authors.forename: Corinne
authors.initials: C
authors.lastname: Leyval
authors.forename: Catherine
authors.initials: C
authors.lastname: Joulian
mesh_terms: D001151:Arsenic / Q000032:analysis / Q000378:metabolism; D018053:Arsenites / Q000378:metabolism*; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005602:France; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D045483:Rivers / Q000737:chemistry / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014873:Water Pollutants / Q000378:metabolism*; D014877:Water Pollution, Chemical
chemical_list: D018053:Arsenites; D014873:Water Pollutants; D010088:Oxidoreductases; C078179:arsenite oxidase; D001151:Arsenic
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