The effect of adrenomedullin and cold stress on interleukin-6 levels in some rat tissues.
Stress known to stimulate sympathetic activity, as well as the hypothalamic-pituitary-adrenal axis (HPA), produces a significant increase in adrenomedullin (AdM) levels, suggesting a regulatory or protective role for AdM in countering HPA activation that follows a variety of stressors. Stressors can modulate the secretion of proinflammatory cytokines. Interleukin (IL)-6 is a potent activator of the HPA and appears to play a pathogenic role in conditions related to stress. In the present study, we investigated the administration of AdM on IL-6 levels in cold exposed rats. Male Wistar rats were divided into four groups as control, adrenomedullin treatment, cold stress and cold stress+adrenomedullin-treated groups. In the adrenomedullin-treated group, animals received intraperitoneal (i.p.) injection of adrenomedullin (2000 ng/kg body weight) once a day for a week. For the cold stress exposure the rats were kept in separate cages at 10 degrees C for a week. Control group rats were kept in laboratory conditions. The concentration of IL-6 was determined using an enzyme-linked immunosorbent assay (ELISA) kit. When compared to control, IL-6 levels increased significantly in the cold stress- and adrenomedullin-treated groups (P<0.05). Administration of AdM in addition to cold stress decreased IL-6 levels in lung and liver, but increased in brain and heart when compared to control (P<0.05). The results suggest that cold stress may induce increase of rat proinflammatory cytokine IL-6 and adrenomedullin may play a regulatory or protective role for cold stress.
Yildirim
N C
NC
Tunceli University, Faculty of Engineering, Department of Environmental Engineering, Tunceli, Turkey. nurancyildirim@tunceli.edu.tr
Yurekli
M
M
D053607:Adrenomedullin / Q000633:toxicity*; D000818:Animals; D001923:Brain Chemistry; D003080:Cold Temperature / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay; D015850:Interleukin-6 / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D008168:Lung / Q000737:chemistry*; D008297:Male; D009206:Myocardium / Q000737:chemistry*; D009928:Organ Specificity; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000276:immunology*
D015850:Interleukin-6; D053607:Adrenomedullin
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