Purification of FANCD2 sub-complexes.
Fanconi anaemia (FA) is a recessive genetic disorder characterized by bone marrow failure, birth defects and cancer. Cells from FA patients are particularly defective in removing DNA interstrand crosslinks. We have developed a working chromatography purification scheme for FANCD2, a pivotal player in the FA DNA repair pathway, to facilitate identification of FANCD2 interacting partners. In doing so, at least three distinct FANCD2 subcomplexes were found to be present, designated as large, middle, and small complexes. The small complex is composed of tetramer of FANCD2 polypeptides, which may be the building block for other FANCD2 subcomplexes.
Zhi
Gang
G
Department of Microbiology, University of Virginia Health System, Virginia, Charlottesville, VA, USA.
Chen
Xiaoyong
X
Newcomb
William
W
Brown
Jay
J
Semmes
Oliver J
OJ
Kupfer
Gary M
GM
D002850:Chromatography, Gel / Q000379:methods; D005199:Fanconi Anemia / Q000378:metabolism*; D052236:Fanconi Anemia Complementation Group D2 Protein / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D008854:Microscopy, Electron
C499905:FANCD2 protein, human; D052236:Fanconi Anemia Complementation Group D2 Protein
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