title: Development of a stabilizer for lyophilization of an attenuated duck viral hepatitis vaccine.
abstract: The live attenuated vaccine against duck viral hepatitis currently available in Korea requires special freezers for storage and transportation with extra costs involved. The development of a lyophilization stabilizer for live attenuated duck viral hepatitis virus (DHV) vaccines, therefore, has been highly recommended for the wider application of the vaccines. Four conventional vaccine stabilizer formulations containing a disaccharide, such as lactose, trehalose, or sucrose, and new formulations containing sorbitol were tested for their efficacy in stabilizing a new attenuated DHV type 3 vaccine candidate under different storage temperatures, 4 and 37 degrees C. The vaccine virus and each stabilizer formulation were combined and submitted to lyophilization and the viability of the virus was measured in 7-d-old specific-pathogen-free chicken embryos by determining the 50% egg lethal dose. Stabilizer formulations containing 2, 4, or 8% sorbitol preserved the viability of the vaccine virus much better than the other stabilizer formulations and 2% sorbitol was the optimal concentration in a standard stabilizing buffer, phosphate glutamate gelatin (0.0038 M KH2PO4, 0.0071 M K2HPO4, 0.0049 M monosodium L-glutamate, and 0.5% gelatin). The results demonstrate that the stabilizer formulation containing 2% sorbitol and 0.5% gelatin can be used for convenient storage and transportation of live DHV vaccines.
authors.affiliation: National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries, Anyang, Gyeonggi, 430-824, Korea.
authors.forename: M S
authors.initials: MS
authors.lastname: Kang
authors.forename: H
authors.initials: H
authors.lastname: Jang
authors.forename: M C
authors.initials: MC
authors.lastname: Kim
authors.forename: M J
authors.initials: MJ
authors.lastname: Kim
authors.forename: S J
authors.initials: SJ
authors.lastname: Joh
authors.forename: J H
authors.initials: JH
authors.lastname: Kwon
authors.forename: Y K
authors.initials: YK
authors.lastname: Kwon
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004356:Drug Storage; D004372:Ducks; D005079:Excipients; D005612:Freeze Drying; D006524:Hepatitis, Viral, Animal / Q000517:prevention & control*; D011201:Poultry Diseases / Q000517:prevention & control* / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D014761:Viral Hepatitis Vaccines / Q000737:chemistry / Q000276:immunology*
chemical_list: D005079:Excipients; D014761:Viral Hepatitis Vaccines