A role for S6 kinase and serotonin in postmating dietary switch and balance of nutrients in D. melanogaster.
Balancing intake of diverse nutrients is important for organismal growth, reproduction, and survival. A shift in an organism's optimal diet due to changes in nutritional requirements after developmental or environmental changes is referred to as dietary switch and has been observed in several species. Here we demonstrate that female Drosophila melanogaster also undergo a dietary switch following mating that leads to an increased preference for yeast, the major source of protein in their diet. We also demonstrate that S6 kinase (S6K) and serotonin production are involved in the postmating dietary switch. To further investigate the ability of D. melanogaster to balance nutrient intake, we examined the dietary preferences of adult flies following deprivation of yeast or sucrose. We observe that following conditioning on a diet deficient in either carbohydrates or yeast, D. melanogaster show a strong preference for the deficient nutrient. Furthermore, flies with activated dS6K or flies fed a serotonin precursor exhibit enhanced preference for yeast in this assay. Our results suggest that TOR signaling and serotonin may play an important role in maintaining nutrient balance in D. melanogaster. These studies may contribute to our understanding of metabolic disorders such as obesity and diabetes.
Vargas
Misha A
MA
Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, CA 94945, USA.
Luo
Ningguang
N
Yamaguchi
Atsushi
A
Kapahi
Pankaj
P
D000818:Animals; D004032:Diet; D004331:Drosophila melanogaster / Q000502:physiology*; D005247:Feeding Behavior / Q000502:physiology*; D005260:Female; D005518:Food Preferences / Q000502:physiology*; D008297:Male; D009751:Nutritional Requirements; D019893:Ribosomal Protein S6 Kinases / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D012726:Sexual Behavior, Animal / Q000502:physiology*
D012701:Serotonin; D019893:Ribosomal Protein S6 Kinases
Obes Rev. 2005 May;6(2):133-42
15836464
Curr Biol. 2006 Apr 4;16(7):692-6
16581515
Science. 1977 Dec 16;198(4322):1178-80
929195
Nature. 1995 Jan 19;373(6511):241-4
7816137
Appetite. 2003 Oct;41(2):123-40
14550310
Aging Cell. 2008 Aug;7(4):478-90
18485125
Aging (Albany NY). 2009 Oct 22;1(10):875-80
20157561
J Nutr. 1975 Nov;105(11):1412-21
1195008
Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13289-94
16150727
Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2498-503
18268352
Cell. 2006 Feb 10;124(3):471-84
16469695
Nutr Res Rev. 1997 Jan;10(1):151-79
19094262
Appetite. 1987 Jun;8(3):195-201
3307629
Appetite. 1987 Jun;8(3):163-82
3307627
Life Sci. 1979 Mar 5;24(10):895-903
221766
Life Sci. 1984 Jun 18;34(25):2453-60
6233467
Cell Metab. 2008 Oct;8(4):289-300
18840359
N Engl J Med. 2009 Dec 31;361(27):2669-70
20042759
Am J Physiol. 1992 Sep;263(3 Pt 2):R559-63
1384352
Curr Biol. 2006 Jun 6;16(11):1051-62
16753559
Appetite. 1997 Jun;28(3):201-13
9218094
Curr Biol. 2008 Jul 22;18(14):1062-6
18635354
Genesis. 2002 Sep-Oct;34(1-2):83-5
12324955
Nat Methods. 2005 Nov;2(11):813-5
16278649