title: Caspase-9-dependent decrease of nuclear pore channel hydrophobicity is accompanied by nuclear envelope leakiness.
abstract: Advances in nanomedicine require conceptual understanding of physiological processes. Apoptosis is a fundamental physiological process that is characterized, among other things, by an increased permeability of the nuclear envelope (NE). The latter is a tight transport barrier, known to restrict nuclear delivery rate of therapeutic nanoparticles. Therefore, an understanding of the underlying mechanism that leads to the breakdown of the barrier during apoptosis could stimulate the development of new approaches in gene therapy. We set out to elucidate this mechanism following induction of apoptosis on isolated cell nuclei. We tested the hypothesis whether caspases, mediators of apoptosis, trigger the NE leakiness at the level of the nuclear pore complexes (NPCs) using fluorescence techniques. As the permeability barrier inside the NPC channel is thought to be based on hydrophobic-hydrophobic protein interactions we further investigated the NPC channel hydrophobicity using atomic force microscopy. Caspase-9 was found to induce NE leakiness to large macromolecules. Leakiness was prevented by pretreatment of NPCs with an importin-β mutant, which irreversibly binds and thereby obstructs the NPC channel. Utilizing an ultra-sharp, hydrophobic atomic force microscope tip as a chemical nanosensor that reaches deep into the apoptotic NPC channel, a remarkable decrease of hydrophobic binding sites was detected therein. We conclude that caspase 9 gives rise to NE leakiness by perturbing the hydrophobicity-based barrier inside the NPC channel. This explains the high passive NE permeability in early apoptosis.
  
  
  BACKGROUND
  In this study, biological processes taking place in the nucleus during the course of apoptosis have been monitored using atomic force microscopy-based nanosensors. The conclusion was that one of the caspases, caspase 9 perturbs the hydrophobicity-based barrier inside the nuclear pore complex channel causing nuclear envelope leakiness.
authors.affiliation: Institute of Physiology II, University of Münster, Münster, Germany.
authors.forename: Armin
authors.initials: A
authors.lastname: Kramer
authors.forename: Ivan
authors.initials: I
authors.lastname: Liashkovich
authors.forename: Hans
authors.initials: H
authors.lastname: Oberleithner
authors.forename: Victor
authors.initials: V
authors.lastname: Shahin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053453:Caspase 9 / Q000378:metabolism*; D045304:Cytochromes c / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D009685:Nuclear Envelope / Q000187:drug effects / Q000378:metabolism*; D022022:Nuclear Pore / Q000187:drug effects / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D045304:Cytochromes c; D053453:Caspase 9