title: A modified synthesis and serological evaluation of neoglycoproteins containing the natural disaccharide of PGL-I from Mycobacterium leprae.
abstract: In order to generate substantial amounts of neoglycoconjugate needed for commercialization of diagnostic kits and high-throughput detection of leprosy, we developed a facile and high-yield synthesis of the corresponding disaccharide. Herein, the non-reducing disaccharide segment of phenolic glycolipid I from Mycobacterium leprae, O-(3,6-di-O-methyl-beta-D-glucopyranosyl)-(1-->4)-O-2,3-di-O-methyl-alpha-L-rhamnopyranose was synthesized by an improved procedure. The disaccharide was efficiently conjugated to bovine/human serum albumin, via acyl-azide intermediate, to form natural disaccharide-BSA/HSA neoglycoproteins that showed a high activity in serodiagnosis of leprosy. The disaccharide incorporated into the proteins was accurately measured by MALDI-TOF mass spectrometry. The serological activities of the neoglycoproteins against pooled human lepromatous leprosy sera were measured by ELISA and they were detectable at picogram amounts.
authors.affiliation: Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA. jian.zhang@adatech.com
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Delphi
authors.initials: D
authors.lastname: Chatterjee
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Brennan
authors.forename: John S
authors.initials: JS
authors.lastname: Spencer
authors.forename: Avraham
authors.initials: A
authors.lastname: Liav
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry*; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000737:chemistry*; D006023:Glycoproteins / Q000097:blood / Q000138:chemical synthesis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000942:Antigens, Bacterial; D004187:Disaccharides; D006017:Glycolipids; D006023:Glycoproteins; C045169:phenolic glycolipid I, Mycobacterium leprae
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