title: Human bone marrow stem cell-encapsulating calcium phosphate scaffolds for bone repair.
abstract: Due to its injectability and excellent osteoconductivity, calcium phosphate cement (CPC) is highly promising for orthopedic applications. However, a literature search revealed no report on human bone marrow mesenchymal stem cell (hBMSC) encapsulation in CPC for bone tissue engineering. The aim of this study was to encapsulate hBMSCs in alginate hydrogel beads and then incorporate them into CPC, CPC-chitosan and CPC-chitosan-fiber scaffolds. Chitosan and degradable fibers were used to mechanically reinforce the scaffolds. After 21 days, that the percentage of live cells and the cell density of hBMSCs inside CPC-based constructs matched those in alginate without CPC, indicating that the CPC setting reaction did not harm the hBMSCs. Alkaline phosphate activity increased by 8-fold after 14 days. Mineral staining, scanning electron microscopy and X-ray diffraction confirmed that apatitic mineral was deposited by the cells. The amount of hBMSC-synthesized mineral in CPC-chitosan-fiber matched that in CPC without chitosan and fibers. Hence, adding chitosan and fibers, which reinforced the CPC, did not compromise hBMSC osteodifferentiation and mineral synthesis. In conclusion, hBMSCs were encapsulated in CPC and CPC-chitosan-fiber scaffolds for the first time. The encapsulated cells remained viable, osteodifferentiated and synthesized bone minerals. These self-setting, hBMSC-encapsulating CPC-based constructs may be promising for bone tissue engineering applications.
authors.affiliation: Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, 21201, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Weir
authors.forename: Hockin H K
authors.initials: HH
authors.lastname: Xu
mesh_terms: D000464:Alginates / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D001843:Bone Cements / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D001861:Bone Regeneration / Q000502:physiology*; D002113:Calcification, Physiologic; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D004339:Drug Compounding; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D008422:Materials Testing; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013314:Stress, Mechanical; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D001843:Bone Cements; D002130:Calcium Phosphates; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid; D048271:Chitosan
references.citation: J Biomed Mater Res. 2002;63(1):1-9
references.pmid: 11787022
references.citation: Acta Biomater. 2010 Jan;6(1):218-28
references.pmid: 19563923
references.citation: Eur J Pharm Sci. 2003 Jan;18(1):23-30
references.pmid: 12554069
references.citation: Biomaterials. 2004 Mar;25(6):1029-37
references.pmid: 14615168
references.citation: J Cell Biochem. 1997 Feb;64(2):278-94
references.pmid: 9027588
references.citation: Biomaterials. 2007 Feb;28(6):1071-83
references.pmid: 17123602
references.citation: J Bone Joint Surg Am. 2008 Feb;90 Suppl 1:36-42
references.pmid: 18292355
references.citation: Biomaterials. 2006 Nov;27(32):5480-9
references.pmid: 16857254
references.citation: J Orthop Res. 2004 May;22(3):535-43
references.pmid: 15099632
references.citation: Biomaterials. 2005 May;26(13):1553-63
references.pmid: 15522757
references.citation: J Biomed Mater Res B Appl Biomater. 2010 Apr;93(1):93-105
references.pmid: 20091907
references.citation: J Biomed Mater Res A. 2008 Apr;85(1):218-27
references.pmid: 17688280
references.citation: J Dent Hyg. 2009 Fall;83(4):173-4
references.pmid: 19909633
references.citation: J Biomed Mater Res. 2001 Dec 5;57(3):457-66
references.pmid: 11523041
references.citation: Clin Plast Surg. 1992 Jan;19(1):41-58
references.pmid: 1537227
references.citation: J Biomed Mater Res. 2002 Jul;61(1):47-52
references.pmid: 12001245
references.citation: Tissue Eng. 2004 Sep-Oct;10(9-10):1480-91
references.pmid: 15588407
references.citation: Biomacromolecules. 2004 Sep-Oct;5(5):1720-7
references.pmid: 15360280
references.citation: Macromol Biosci. 2004 Aug 9;4(8):743-65
references.pmid: 15468269
references.citation: J Biomed Mater Res. 1998 Winter;43(4):428-32
references.pmid: 9855201
references.citation: J Biomech. 1985;18(5):317-28
references.pmid: 4008502
references.citation: J Dent Res. 2009 Aug;88(8):681-92
references.pmid: 19734453
references.citation: Biotechnol Prog. 2006 Nov-Dec;22(6):1697-701
references.pmid: 17137320
references.citation: Acta Biomater. 2008 Nov;4(6):1904-15
references.pmid: 18571999
references.citation: J Control Release. 2006 Jun 28;113(2):102-10
references.pmid: 16740332
references.citation: J Oral Maxillofac Surg. 2007 Aug;65(8):1640-7
references.pmid: 17656295
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998 May;85(5):512-6
references.pmid: 9619665
references.citation: Nat Mater. 2008 Oct;7(10):816-23
references.pmid: 18724374
references.citation: J Biomed Mater Res A. 2006 Jun 1;77(3):487-96
references.pmid: 16482548
references.citation: Ann Periodontol. 1996 Nov;1(1):621-66
references.pmid: 9118274
references.citation: J Dent Res. 2008 Jul;87(7):650-4
references.pmid: 18573985
references.citation: Tissue Eng. 2007 Mar;13(3):493-500
references.pmid: 17362133
references.citation: Clin Mater. 1993;14(1):65-88
references.pmid: 10171998
references.citation: Tissue Eng Part A. 2009 Jul;15(7):1695-707
references.pmid: 19119921
references.citation: Biol Pharm Bull. 1996 Nov;19(11):1518-20
references.pmid: 8951178
references.citation: Tissue Eng Part A. 2009 Feb;15(2):243-54
references.pmid: 19193129
references.citation: Acta Biomater. 2009 Sep;5(7):2752-62
references.pmid: 19357005
references.citation: J Biomed Mater Res A. 2004 Mar 15;68(4):773-82
references.pmid: 14986332
references.citation: Transplantation. 1996 Oct 15;62(7):888-93
references.pmid: 8878379
references.citation: Dent Mater J. 1995 Jun;14(1):45-57
references.pmid: 8940545
references.citation: Acta Biomater. 2008 Sep;4(5):1465-71
references.pmid: 18445539
references.citation: Biomaterials. 2009 May;30(14):2675-82
references.pmid: 19187958
references.citation: J Mater Sci Mater Med. 1999 Mar;10(3):135-45
references.pmid: 15348161
references.citation: Biomaterials. 2002 Jan;23(1):193-202
references.pmid: 11763861
references.citation: Acta Biomater. 2010 Mar;6(3):876-85
references.pmid: 19835998
references.citation: J Biomed Mater Res A. 2005 Dec 15;75(4):966-75
references.pmid: 16123976