title: Geraniol and linalool synthases from wild species of perilla.
abstract: Geraniol and linalool synthases were isolated from three pure strains of Perilla hirtella and Perilla setoyensis, which are wild species of perilla. Their amino acid sequences were very similar to those of Perilla citriodora and Perilla frutescens that were reported previously. However, comparison of the sequences of the same functional synthases derived from different species of Perilla demonstrated that the similarities were high among P. citriodora, P. hirtella and P. frutescens, but low between P. setoyensis and any of the others. This result corresponds well with our previous results showing that P. setoyensis is remotely related to the other perilla species. Both geraniol and linalool synthases utilize geranyl diphosphate (GDP) as their catalytic substrate and they were expressed simultaneously in perilla. The linalool synthase is considered to be the enzyme whose metabolite seems not to be oxidized nor reduced in the plant body and the geraniol and limonene synthases are the initial-step-catalyzing enzymes for a variety of oil compounds. The regulation of the substrate flow between them would be interesting for further study.
authors.affiliation: Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-Shimoadachi, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Masumoto
authors.forename: Miyuki
authors.initials: M
authors.lastname: Korin
authors.forename: Michiho
authors.initials: M
authors.lastname: Ito
mesh_terms: D000595:Amino Acid Sequence; D055162:Biocatalysis; D006836:Hydro-Lyases / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D031343:Perilla / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D010744:Phosphoric Monoester Hydrolases; C552193:geraniol synthase, Perilla hirtella; D006836:Hydro-Lyases; C092055:S-linalool synthase