Ultrasensitive aptamer-based protein detection via a dual amplified biocatalytic strategy.
We present an ultrasensitive aptasensor for the electronic monitoring of proteins through a dual amplified strategy in this paper. The target protein thrombin is sandwiched between an electrode surface confined aptamer and an aptamer-enzyme-carbon nanotube bioconjugate. The analytical signal amplification is achieved by coupling the signal amplification nature of multiple enzymes with the biocatalytic signal enhancement of redox-recycling. Our novel dramatic signal amplification strategy, with a detection limit of 8.3fM, shows about 4 orders of magnitude improvement in the sensitivity for thrombin detection compared to other universal single enzyme-based assay. This makes our approach an attractive alternative to other common PCR-based signal amplification in ultralow level of protein detection.
Xiang
Yun
Y
Key Laboratory of Ministry of Education on Luminescence and Real-Time Analysis, School of Chemistry and Chemical Engineering, Southwest University, #2 Tiansheng Road, Chongqing 400715, PR China. yunatswu@swu.edu.cn
Zhang
Yuyong
Y
Qian
Xiaoqing
X
Chai
Yaqin
Y
Wang
Joseph
J
Yuan
Ruo
R
D052158:Aptamers, Peptide / Q000737:chemistry*; D002384:Catalysis; D004867:Equipment Design; D019544:Equipment Failure Analysis; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D052158:Aptamers, Peptide; D011506:Proteins
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