Proliferation and osteoblastic differentiation of bone marrow stem cells: comparison of vertebral body and iliac crest.
Bone marrow stem cells (BMSCs) can be obtained from the vertebral body (VB) and iliac crest (IC) for augmenting spinal arthrodesis. However, it is still not evaluated, which of the two sites would have a better BMSCs potential on Proliferation and osteoblastic differentiation is still not evaluated. Fourteen patients (10 men and 4 women) undergoing posterolateral lumbar arthrodesis and pedicle screw instrumentation were involved. The mean age was 54.7 years (range 31-75 years). Bone marrow aspirates were obtained from the vertebral body through the bilateral pedicle and were quantified relative to matched, bilateral aspirates from the iliac crest that were obtained from the same patient and at the same time. The mononuclear cell count and concentration of BMSCs were calculated and compared. Proliferation and osteoblastic differentiation of each of the BMSCs were characterized using biochemical and molecular biology techniques. Concentration (cells/mL) of BMSCs from VB and IC were 3.73 × 10(3) and 3.19 × 10(3), respectively (P > 0.05). VB and IC exhibited similar proliferation pattern at 3, 5 and 7 days, but BMSCs from the VB exhibited an increased mineralization staining with Alizarin Red S at 14 days. BMSCs from both anatomic sites expressed comparable levels of CD29, CD34, CD44, CD90 and CD105. VB and IC displayed similar levels of expression of ALP, type I collagen and osterix, but VB expressed higher level of osteocalcin and Runx-2, especially at 14 and 21 days. Our studies show that BMSCs from VB have osteogenic differentiation potential similar to IC. Based on these findings, we suggest that BMSCs from VB would be comparable candidates for osseous graft supplementation especially in spinal fusion procedures.
Min
Woo-Kie
WK
Department of Orthopedic Surgery, School of Medicine, Kyungpook National University, 50 Samduk 2-ga, Jung-gu, Daegu 700-712, Korea.
Bae
Jae-Sung
JS
Park
Byung-Chul
BC
Jeon
In-Ho
IH
Jin
Hee Kyung
HK
Son
Min-Jung
MJ
Park
Eui Kyun
EK
Kim
Shin-Yoon
SY
D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D016026:Bone Marrow Transplantation / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007085:Ilium / Q000166:cytology* / Q000502:physiology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D013131:Spine / Q000166:cytology* / Q000502:physiology / Q000601:surgery; D033581:Stem Cell Transplantation / Q000379:methods*
Br J Haematol. 2003 Aug;122(3):506-17
12877680
Clin Orthop Relat Res. 2003 Feb;(407):102-18
12567137
Clin Orthop Relat Res. 1991 May;(266):259-70
2019059
Stem Cells. 2003;21(2):190-9
12634415
J Bone Joint Surg Am. 1995 Jun;77(6):940-56
7782368
J Bone Joint Surg Am. 2005 Dec;87(12):2655-2661
16322615
Bone. 1997 Jul;21(1):1-6
9213001
Curr Opin Biotechnol. 2004 Oct;15(5):406-10
15464369
J Cell Biochem. 2003 May 1;89(1):56-66
12682908
Transplantation. 2004 Sep 27;78(6):925-9
15385815
Neurosurgery. 2002 Sep;51(3):854-5; author reply 855
12229871
J Orthop Res. 1997 Jul;15(4):546-57
9379264
Neurosci Lett. 2004 May 27;362(3):200-3
15158014
J Biomed Mater Res. 1999 Jan;44(1):98-108
10397909
Spine (Phila Pa 1976). 2006 Jan 1;31(1):83-9
16395182
Cell Physiol Biochem. 2004;14(4-6):311-24
15319535
J Orthop Trauma. 1989;3(3):192-5
2809818
Biochem J. 1996 Jul 1;317 ( Pt 1):59-64
8694787
Cell Tissue Res. 2003 Sep;313(3):281-90
12883998
Stem Cells. 2006 Apr;24(4):1113-20
16282439
J Cell Biochem. 1997 Feb;64(2):295-312
9027589
J Bone Joint Surg Am. 1997 Nov;79(11):1699-709
9384430
J Cell Physiol. 1994 Mar;158(3):555-72
8126078
J Biol Chem. 1995 Apr 21;270(16):9420-8
7721867
Trends Biotechnol. 2002 Aug;20(8):351-6
12127283
Stem Cells. 2006 Nov;24(11):2412-9
16840552
J Orthop Res. 2001 Jan;19(1):117-25
11332607
Nat Med. 1999 Mar;5(3):309-13
10086387