Polymorphism analysis and evaluation of nine non-CODIS STR loci in the Han population of Southern China.
BACKGROUND
Knowledge of allele and genotype frequencies is an essential prerequisite to the use of any human polymorphism in forensic work.


OBJECTIVE
To study the genetic polymorphism and evaluate the application value of nine STR loci.


METHODS
Genotyping of nine STR loci, including D11S2368, D12S391, D13S325, D18S1364, D22-GATA198B05, D6S1043, D2S1772, D7S3048 and D8S1132, of 1050 unrelated individuals was performed with the STR_Typer_10_v1 kit and Genetic Analyzer 3100 and analyzed with PowerState V12.xls and Arlequin ver 3.11 analyzing software.


RESULTS
Allele frequency distribution was statistically analyzed and Hardy-Weinberg equilibrium determined. Several common parameters used in forensic sciences were found: the heterozygosity (H) ranged from 0.827 to 0.892; the matching probability (MP) ranged from 0.029 to 0.074; the power of discrimination (PD) ranged from 0.926 to 0.971; the power of exclusion (PE) ranged from 0.649 to 0.779; the polymorphic information content (PIC) ranged from 0.77 to 0.86; and the typical paternity index (TPI) ranged from 2.88 to 4.62.


CONCLUSIONS
The results indicate that nine STR loci are high polymorphic among the Han population in Southern China. This set of polymorphic STR loci is a useful tool in forensic paternity testing and anthropological study.
Tong
Da Yue
da Y
Department of Forensic Biology, Sun Yat-Sen University, Zhongshan 2 Road #74, Guangzhou, China.
Wu
Xin Yao
XY
Sun
Hong Yu
HY
Zhao
Hu
H
Lu
Hui Ling
HL
D000328:Adult; D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D002681:China; D005006:Ethnicity / Q000235:genetics; D005260:Female; D053803:Forensic Genetics; D005784:Gene Amplification; D005787:Gene Frequency; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D005819:Genetic Markers