Single point vs. mapping approach for spectral cytopathology (SCP).
In this paper we describe the advantages of collecting infrared microspectral data in imaging mode opposed to point mode. Imaging data are processed using the PapMap algorithm, which co-adds pixel spectra that have been scrutinized for R-Mie scattering effects as well as other constraints. The signal-to-noise quality of PapMap spectra will be compared to point spectra for oral mucosa cells deposited onto low-e slides. Also the effects of software atmospheric correction will be discussed. Combined with the PapMap algorithm, data collection in imaging mode proves to be a superior method for spectral cytopathology.
Schubert
Jennifer M
JM
Northeastern University, Department of Chemistry and Chemical Biology, Boston, MA 02115, USA.
Mazur
Antonella I
AI
Bird
Benjamin
B
Miljković
Milos
M
Diem
Max
M
D000818:Animals; D002478:Cells, Cultured / Q000737:chemistry* / Q000145:classification*; D006801:Humans; D008853:Microscopy / Q000379:methods*; D013055:Spectrophotometry, Infrared / Q000379:methods*
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