Development of a gene transfer system for the mycelia of Flammulina velutipes Fv-1 strain.
To develop a gene transformation method for Flammulina velutipes, we constructed a vector with hph gene under control of the trp1 gene promoter. The vector was integrated into protoplast derived from mycelia by the calcium-polyethylene glycol method, as it has not been reported for F. velutipes. Transformation efficiency was much improved when transformation was performed by the restriction enzyme mediated integration method.
Maehara
Tomoko
T
Food Biotechnology Division, National Food Research Institute, Tsukuba, Ibaraki, Japan.
Yoshida
Makoto
M
Ito
Yasuhiro
Y
Tomita
Shizuko
S
Takabatake
Koji
K
Ichinose
Hitomi
H
Kaneko
Satoshi
S
D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D055393:Flammulina / Q000166:cytology / Q000235:genetics*; D018014:Gene Transfer Techniques; D025282:Mycelium / Q000235:genetics*; D011092:Polyethylene Glycols / Q000737:chemistry; D011523:Protoplasts / Q000378:metabolism
D011092:Polyethylene Glycols; D002118:Calcium