Human cytomegalovirus infection among patients living with AIDS in a tertiary level hospital in India.
OBJECTIVE
Detecting the prevalence of human cytomegalovirus (CMV) coinfection in HIV-infected individuals attending a tertiary care hospital in Delhi by using 3 laboratory tests namely pp65 antigenemia assay, CMV DNA polymerase chain reaction (PCR), and immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) for anti-CMV antibody.


METHODS
HIV seropositive patients with CD4 count < or = 100 cells/mm(3) were included as cases and HIV-negative healthy individuals as controls. Blood samples were collected from all participants and 3 tests were performed.


METHODS
pp65 antigenemia assay and IgM ELISA were performed by commercially available kits; PCR was performed by an in-house nested PCR method.


RESULTS
In all, 7.1%, 5.7%, and 2.9% were positive for pp65 antigenemia, CMV DNA, and IgM antibody against CMV, respectively. On the basis of pp65 and/or PCR positivity, it can be said that active CMV coinfection was present in 8.57% of the cases.


CONCLUSIONS
In the post-antiretroviral therapy (ART) era, CMV coinfection in HIV-infected patients has significantly declined.
Chakravarti
Anita
A
Department of Microbiology, Maulana Azad Medical College, New Delhi, India. anitachakravarti@gmail.com
Tewari
Sayani
S
Bhalla
Preena
P
D017088:AIDS-Related Opportunistic Infections / Q000453:epidemiology* / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000150:complications / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D017726:Cytomegalovirus Retinitis / Q000150:complications / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D015658:HIV Infections / Q000150:complications / Q000453:epidemiology* / Q000821:virology; D006761:Hospitals; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007194:India / Q000453:epidemiology; D010750:Phosphoproteins / Q000097:blood; D016133:Polymerase Chain Reaction / Q000379:methods; D014763:Viral Matrix Proteins / Q000097:blood
D004279:DNA, Viral; D007075:Immunoglobulin M; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa