title: [Construction of conjugal transfer system of Streptomyces venezuelae var. Qinlingensis and the expression of vitreoscilla hemoglobin].
abstract: OBJECTIVE
  To Construct of conjugal transfer system of Streptomyces venezuelae var. Qinlingensis and express of vitreoscilla hemoglobin.
  
  
  METHODS
  Intergeneric genetic transfer system was based upon plasmid pSET152 and pHZ1358 from Donor E. coli ET12567 (pUZ8002) to Streptomyces venezuelae var. Qinlingensis.
  
  
  RESULTS
  Our data showed that Intergeneric genetic transfer system was demonstrated and optimized. Integrating plasmid pJD100 containing Perm E and construction gene of vhb, constructed by SOE-PCR, was transformed to ET12567 (pUZ8002), and then transfered to Streptomyces venezuelae var. Qinlingensis by conjugation. The integrating expression of vhb gene in Streptomyces venezuelae var. Qinlingensis was verified by PCR and CO binding diference spectrum.
  
  
  CONCLUSIONS
  In summary, intergeneric genetic transfer system was demonstrated and optimized, and this is the first report to express vhb gene in Streptomyces venezuelae var. Qinlingensis.
authors.affiliation: College of Plant Protection and Shanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, China. gaofeng007xfg@163.com
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Taichun
authors.initials: T
authors.lastname: Chen
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wang
authors.forename: Derong
authors.initials: D
authors.lastname: An
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D015139:Blotting, Southern; D003227:Conjugation, Genetic / Q000235:genetics*; D006358:Hot Temperature; D016133:Polymerase Chain Reaction; D013171:Spores, Bacterial / Q000502:physiology; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D054793:Truncated Hemoglobins / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla