title: Infestation of sheep with Psoroptes ovis, the sheep scab mite, results in recruitment of Foxp3(+) T cells into the dermis.
abstract: Regulatory T cells (Tregs) play a central role in maintenance of immune homeostasis by controlling harmful immune responses to inappropriate antigens and are thought to play a key role in modulating hypersensitivity reactions. Infestation of sheep with Psoroptes ovis results in a pronounced cutaneous hypersensitivity-type response, which appears to be crucial for mite survival. We hypothesize that (i) Tregs are involved in sheep scab lesions and (ii) Treg responses may crucially affect lesion development and subsequent mite survival. Foxp3 is a key transcription factor required for generation and maintenance of Tregs in rodents and humans, and is the most widely used marker for Tregs in these species. In this study, we sequence ovine foxp3 and show that it exhibits a high degree of homology with foxp3 from other species. Using a validated immunohistochemical staining technique, we demonstrate that infestation of sheep with P. ovis results in an influx of Foxp3(+) T cells into the skin. Future work will investigate the regulatory function of ovine Foxp3(+) T cells and determine whether the quality of the Treg response to P. ovis plays a role in individual susceptibility to the mite.
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan Penicuik, Midlothian, EH26 OPZ, UK. tom.mcneilly@moredun.ac.uk
authors.forename: T N
authors.initials: TN
authors.lastname: McNeilly
authors.forename: J
authors.initials: J
authors.lastname: McIntyre
authors.forename: D
authors.initials: D
authors.lastname: Frew
authors.forename: D J
authors.initials: DJ
authors.lastname: Griffiths
authors.forename: S R
authors.initials: SR
authors.lastname: Wattegedera
authors.forename: A
authors.initials: A
authors.lastname: van den Broek
authors.forename: J F
authors.initials: JF
authors.lastname: Huntley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020405:Dermis / Q000276:immunology* / Q000469:parasitology* / Q000473:pathology; D051858:Forkhead Transcription Factors / Q000032:analysis* / Q000235:genetics; D007150:Immunohistochemistry / Q000379:methods; D008924:Mite Infestations / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D008969:Molecular Sequence Data; D040042:Psoroptidae / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000737:chemistry / Q000276:immunology*
chemical_list: D051858:Forkhead Transcription Factors