Wingless modulates the ligand independent traffic of Notch through Dishevelled.
Here we investigate the structural and functional basis of the interactions between Notch and Wingless signalling in Drosophila. Using yeast-two-hybrid and pull-down assays we show that Notch can bind directly a form of Dishevelled that is stabilized upon Wingless signalling. Moreover, we show that the mechanism by which Wingless signalling is able to downregulate Notch is by promoting its ligand-independent traffic to a compartment where it is degraded and that this activity depends on Dishevelled.
Muñoz-Descalzo
Silvia
S
Department of Genetics, University of Cambridge, Cambridge, UK.
Sanders
Phil G T
PG
Montagne
Chrystelle
C
Johnson
Ruth I
RI
Balayo
Tina
T
Arias
Alfonso Martinez
AM
D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D000818:Animals; D000072261:Dishevelled Proteins; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism* / Q000502:physiology*; D005260:Female; D008024:Ligands; D008297:Male; D010750:Phosphoproteins / Q000502:physiology*; D021381:Protein Transport / Q000502:physiology; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D051155:Wnt1 Protein / Q000502:physiology*
D048868:Adaptor Proteins, Signal Transducing; D000072261:Dishevelled Proteins; D029721:Drosophila Proteins; D008024:Ligands; C497759:N protein, Drosophila; D010750:Phosphoproteins; D051880:Receptors, Notch; D051155:Wnt1 Protein; C000606588:dsh protein, Drosophila; C074622:wg protein, Drosophila