Hymenobacter psychrophilus sp. nov., a psychrophilic bacterium isolated from soil.
A non-motile, rod-shaped, red-pigmented bacterium, designated strain BZ33r(T), was isolated from soil of an industrial site. Cells stained Gram-negative and were aerobic and psychrophilic, showing good growth at 1-20 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ33r(T) was related to members of the genus Hymenobacter and had highest sequence similarity with Hymenobacter aerophilus DSM 13606(T) (97.5 %). The predominant cellular fatty acids were anteiso-C(15 : 0) (20.3 %), summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH; 20.2 %), iso-C(15 : 0) (20.0 %), summed feature 4 (iso-C(17 : 1) I and/or anteiso-C(17 : 1) B; 8.2 %) and C(16 : 1)ω5c (7.9 %). Strain BZ33r(T) had MK-7 as the major menaquinone. The polyamine pattern contained sym-homospermidine as the major compound with moderate amounts of spermidine. Phosphatidylethanolamine, three unknown aminophospholipids, two unknown aminolipids, two unknown glycolipids and five unknown polar lipids were present in the polar lipid profile. The G+C content of the DNA was 61.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain BZ33r(T) is considered to represent a novel species of the genus Hymenobacter, for which the name Hymenobacter psychrophilus sp. nov. is proposed. The type strain is BZ33r(T) ( = DSM 22290(T)  = CGMCC 1.8975(T)  = LMG 25548(T)).
Zhang
De-Chao
DC
Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
Busse
Hans-Jürgen
HJ
Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210 Vienna, Austria.
Liu
Hong-Can
HC
China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Zhou
Yu-Guang
YG
China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Schinner
Franz
F
Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
Margesin
Rosa
R
Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
D000332:Aerobiosis; D001482:Base Composition; D016000:Cluster Analysis; D003080:Cold Temperature; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011073:Polyamines / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D010860:Pigments, Biological; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2