title: Enzymatic analysis of Hemiscorpius lepturus scorpion venom using zymography and venom-specific antivenin.
abstract: Hemiscorpius lepturus envenomation exhibits various pathological changes in the affected tissues, including skin, blood cells, cardiovascular and central nervous systems. The enzymatic activity and protein component of the venom have not been described previously. In the present study, the electrophoretic profile of H. lepturus venom was determined by SDS-PAGE (12 and 15%), resulting in major protein bands at 3.5-5, 30-35 and 50-60 kDa. The enzymatic activities of the venom was, for the first time, investigated using various zymography techniques, which showed the gelatinolytic, caseinolytic, and hyaluronidase activities mainly at around 50-60 kDa, 30-40 kDa, and 40-50 kDa, respectively. Among these, the proteolytic activities was almost completely disappeared in the presence of a matrix metalloproteinase inhibitor, 1, 10-phenanthroline. Antigen-antibody interactions between the venom and its Iranian antivenin was observed by Western blotting, and it showed several antigenic proteins in the range of 30-160 kDa. This strong antigen-antibody reaction was also demonstrated through an enzyme-linked immunosorbent assay (ELISA). The gelatinase activity of the venom was suppressed by Razi institute polyvalent antivenin, suggesting the inhibitory effect of the antivenin against H. lepturus venom protease activities. Prudently, more extensive clinical studies are necessary for validation of its use in envenomed patients.
authors.affiliation: Department of Pharmacology and Toxicology, Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: Ramin
authors.initials: R
authors.lastname: Seyedian
authors.forename: Mohammad Hassan
authors.initials: MH
authors.lastname: Pipelzadeh
authors.forename: Amir
authors.initials: A
authors.lastname: Jalali
authors.forename: Euikyung
authors.initials: E
authors.lastname: Kim
authors.forename: Hyunkyoung
authors.initials: H
authors.lastname: Lee
authors.forename: Changkeun
authors.initials: C
authors.lastname: Kang
authors.forename: Mijin
authors.initials: M
authors.lastname: Cha
authors.forename: Eun-Tae
authors.initials: ET
authors.lastname: Sohn
authors.forename: Eun-Sun
authors.initials: ES
authors.lastname: Jung
authors.forename: Ali Hassan
authors.initials: AH
authors.lastname: Rahmani
authors.forename: Abbas Zare
authors.initials: AZ
authors.lastname: Mirakabady
mesh_terms: D000818:Animals; D000997:Antivenins / Q000737:chemistry* / Q000494:pharmacology; D015153:Blotting, Western; D002364:Caseins / Q000737:chemistry; D002417:Cattle; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005780:Gelatin / Q000737:chemistry; D018093:Gelatinases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification; D006820:Hyaluronic Acid / Q000737:chemistry; D006821:Hyaluronoglucosaminidase / Q000737:chemistry / Q000302:isolation & purification; D008666:Metalloendopeptidases / Q000737:chemistry / Q000302:isolation & purification; D012604:Scorpion Venoms / Q000737:chemistry / Q000201:enzymology* / Q000494:pharmacology; D012605:Scorpions / Q000201:enzymology*; D013552:Swine
chemical_list: D000997:Antivenins; D002364:Caseins; D004791:Enzyme Inhibitors; D012604:Scorpion Venoms; D005780:Gelatin; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase; D018093:Gelatinases; D008666:Metalloendopeptidases; C030895:caseinase