Actinophytocola timorensis sp. nov. and Actinophytocola corallina sp. nov., isolated from soil.
Two actinomycete strains, ID05-A0653(T) and ID06-A0464(T), were isolated from soils of West Timor and Lombok island, respectively, in Indonesia. 16S rRNA gene sequence analysis clearly demonstrated that the isolates belonged to the family Pseudonocardiaceae and were closely related to the genus Actinophytocola. Strains ID05-A0653(T) and ID06-A0464(T) exhibited 98.1 and 98.2 % 16S rRNA gene sequence similarity, respectively, with Actinophytocola oryzae GMKU 367(T). The isolates grew well on ISP media and produced white aerial mycelium. Short spore chains were formed directly on the substrate mycelium. The isolates contained meso-diaminopimelic acid, arabinose and galactose as cell-wall components, MK-9(H(4)) as the sole isoprenoid quinone, iso-C(16 : 0) as the major cellular fatty acid and phosphatidylethanolamine as the diagnostic polar lipid. The DNA G+C contents of strains ID05-A0653(T) and ID06-A0464(T) were 69.7 and 71.2 mol%, respectively. On the basis of phenotypic characteristics, DNA-DNA relatedness and 16S rRNA gene sequence comparisons, strains ID05-A0653(T) and ID06-A0464(T) each represent a novel species of the genus Actinophytocola, for which the names Actinophytocola timorensis sp. nov. (type strain ID05-A0653(T)  = BTCC B-673(T)  = NBRC 105524(T)) and Actinophytocola corallina sp. nov. (type strain ID06-A0464(T)  = BTCC B-674(T)  = NBRC 105525(T)) are proposed.
Otoguro
Misa
M
NITE Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Yamamura
Hideki
H
Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu 400-8511, Japan.|NITE Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Tamura
Tomohiko
T
NITE Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Irzaldi
Rohmatussolihat
R
Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
Ratnakomala
Shanti
S
Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
Ridwan
Roni
R
Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
Kartina
Gina
G
Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
Triana
Evi
E
Research Center for Biology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
Nurkanto
Arif
A
Research Center for Biology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
Lestari
Yulin
Y
Department of Biology, Faculty of Mathematics and Natural Science, Bogor Agricultural University, Jl. Raya pajajaran, Bogor 16144, Indonesia.
Lisdiyanti
Puspita
P
Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
Widyastuti
Yantyati
Y
Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
Ando
Katsuhiko
K
NITE Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001089:Arabinose / Q000032:analysis; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005690:Galactose / Q000032:analysis; D007214:Indonesia; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000166:cytology
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid; D001089:Arabinose; D005690:Galactose