Reconstruction of the UDP-N-acetylglucosamine biosynthetic pathway in cell-free system.
A functional glucokinase (YqgR), N-acetylglucosamine-phosphate mutase (Agm1) and N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) were synthesized simultaneously in an Escherichia coli cell-free system. These three enzymes were coupled to reconstruct a biosynthetic pathway which could produce UDP-N-acetylglucosamine with N-acetylglucosamine as the substrate.
Zhou
Jiewen
J
Institute of Biological Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
Huang
Lei
L
Lian
Jiazhang
J
Sheng
Jiayuan
J
Cai
Jin
J
Xu
Zhinan
Z
D000117:Acetylglucosamine / Q000378:metabolism*; D001412:Bacillus subtilis / Q000201:enzymology; D053898:Biosynthetic Pathways; D004926:Escherichia coli / Q000201:enzymology; D005941:Glucokinase / Q000378:metabolism; D066298:In Vitro Techniques; D019751:Intramolecular Transferases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D017855:Transferases (Other Substituted Phosphate Groups) / Q000378:metabolism; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000096:biosynthesis*
D014537:Uridine Diphosphate N-Acetylglucosamine; D005941:Glucokinase; D017855:Transferases (Other Substituted Phosphate Groups); C023222:UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase; D019751:Intramolecular Transferases; D000117:Acetylglucosamine