[Inhibitory effect of SHP-1 gene transfer on the proliferation of breast cancer cell line MDA-MB-231].
OBJECTIVE
To observe SHP-1 protein expression in breast cancer cell line MDA-MB-231 before and after SHP-1 gene transfer and its effect on the proliferation of MDA-MB-231 cells.


METHODS
The eukaryotic expression vector pEGFP-C3-SHP-1 was constructed and transfected into breast cancer cell line MDA-MB-231 via Lipofectamine 2000, and the positive clones were selected using G418. SHP-1 expression in MDA-MB-231 cells was detected with immunocytochemistry and Western blotting, and the cell growth curve was observed using MTT assay.


RESULTS
SHP-1 was highly expressed in transfected MDA-MB-231 cells, whose proliferation was significantly inhibited (P<0.05).


CONCLUSIONS
SHP-1 gene transfer into MDA-MB-231 cells results in inhibition of the cell proliferation.
Xie
Ya-lin
YL
Oncology Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Liang
Ji-zhen
JZ
Liu
Fei-ye
FY
Zhang
Jun-yi
JY
Xie
Jian-ming
JM
Luo
Rong-cheng
RC
Li
Rong
R
D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000235:genetics* / Q000378:metabolism
C509249:PTPN6 protein, human; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6