Induction of cytotoxicity and apoptosis in mouse blastocysts by silver nanoparticles.
Silver nanoparticles (nanoAg) are antibacterial materials widely used in various products and medical supplies. In this report, we examined the cytotoxic effects of nanoAg on mouse embryos at the blastocyst stage, subsequent embryonic attachment and outgrowth in vitro, and in vivo implantation by embryo transfer. Blastocysts treated with 50 microM nanoAg exhibited significantly increased apoptosis and a corresponding decrease in total cell number. Importantly, the implantation success rate of blastocysts pretreated with nanoAg was lower than that of their control counterparts. Moreover, in vitro treatment with 50 microM nanoAg was associated with increased resorption of post-implantation embryos and decreased fetal weight. Our results collectively indicate that in vitro exposure to nanoAg induces apoptosis and retards early post-implantation development after transfer to host mice. However, nanoAg-stimulated embryonic cytotoxicity appeared lower than that induced by the Ag+ ion. The results collectively show that nanoAg has the potential to induce embryo cytotoxicity. Further studies are required to establish effective protection strategies against the cytotoxic effects of these nanoparticles.
Li
Po-Wn
PW
Chung Yuan Christian University, Chung Li, Taiwan.
Kuo
Tai-Hung
TH
Chang
Ji-Hao
JH
Yeh
Jui-Ming
JM
Chan
Wen-Hsiung
WH
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects*; D001755:Blastocyst / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D010064:Embryo Implantation; D004624:Embryo Transfer; D047108:Embryonic Development / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D012834:Silver / Q000737:chemistry* / Q000633:toxicity*
D000900:Anti-Bacterial Agents; D012834:Silver