title: [Inhibitory effect of SHP-1 gene transfer on the proliferation of breast cancer cell line MDA-MB-231].
abstract: OBJECTIVE
  To observe SHP-1 protein expression in breast cancer cell line MDA-MB-231 before and after SHP-1 gene transfer and its effect on the proliferation of MDA-MB-231 cells.
  
  
  METHODS
  The eukaryotic expression vector pEGFP-C3-SHP-1 was constructed and transfected into breast cancer cell line MDA-MB-231 via Lipofectamine 2000, and the positive clones were selected using G418. SHP-1 expression in MDA-MB-231 cells was detected with immunocytochemistry and Western blotting, and the cell growth curve was observed using MTT assay.
  
  
  RESULTS
  SHP-1 was highly expressed in transfected MDA-MB-231 cells, whose proliferation was significantly inhibited (P<0.05).
  
  
  CONCLUSIONS
  SHP-1 gene transfer into MDA-MB-231 cells results in inhibition of the cell proliferation.
authors.affiliation: Oncology Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Ya-lin
authors.initials: YL
authors.lastname: Xie
authors.forename: Ji-zhen
authors.initials: JZ
authors.lastname: Liang
authors.forename: Fei-ye
authors.initials: FY
authors.lastname: Liu
authors.forename: Jun-yi
authors.initials: JY
authors.lastname: Zhang
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Xie
authors.forename: Rong-cheng
authors.initials: RC
authors.lastname: Luo
authors.forename: Rong
authors.initials: R
authors.lastname: Li
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000235:genetics* / Q000378:metabolism
chemical_list: C509249:PTPN6 protein, human; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6