title: [In vitro activity of baicalin against non-albicans Candida biofilms].
abstract: OBJECTIVE
  To investigate the effects of baicalin against Candida glabrata, C. parapsilosis, C. krusei and C. guilliermondii biofilms.
  
  
  METHODS
  96-well microtitre plates were used to set up the biofilms; microdilution method was applied to detect minimal inhibitory concentration (MIC) of baicalin for the four non-albians Candida, and XTT reduction assay was adopted to determine sessile minimal inhibitory concentration (SMIC) of baicalin against the four isolates and to detect the effects on adhesion of the fungal cells.
  
  
  RESULTS
  MICs of baicalin for the four non-albians Candida cells were 125, 250, 125, 62.5 mg L(-1), respectively. The four non-albians Candida could form mature biofilms on 96-well microtitre plates. SMIC50 of baicalin for the four isolates were > 1000, 500, 125, 250 mg x L(-1), respectively. SMIC80 for the four isolates were greater than or equal to 1000 mg x L(-1). Baicalin showed potent inhibitory effects on adhesion of the four non-albians Candida cells.
  
  
  CONCLUSIONS
  Baicalin displays substantial inhibitory effects on C. parapsilosis, C. krusei and C. guilliermondii biofilm.
authors.affiliation: School of Integrated Traditional and Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China. ahwcz63@sina.com
authors.forename: Changzhong
authors.initials: C
authors.lastname: Wang
authors.forename: Huijuan
authors.initials: H
authors.lastname: Cheng
authors.forename: Xiongfei
authors.initials: X
authors.lastname: Zhang
authors.forename: Shengli
authors.initials: S
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Guan
authors.forename: Lihua
authors.initials: L
authors.lastname: Yu
authors.forename: Yun
authors.initials: Y
authors.lastname: Yun
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D002175:Candida / Q000187:drug effects* / Q000502:physiology; D005419:Flavonoids / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests
chemical_list: D005419:Flavonoids; C038044:baicalin