title: Snf1/AMPK promotes S-phase entrance by controlling CLB5 transcription in budding yeast.
abstract: The Saccharomyces cerevisiae Snf1 protein kinase has been reported to be required for adaptation to glucose limitation and for growth on non-fermentable carbon sources. Here we present novel findings indicating that Snf1, the key regulator of cellular energy, is also involved in yeast cell cycle control. The lack of Snf1 α-catalytic subunit down-regulates the growth rate and CLB5 expression, delaying Sld2 phosphorylation and G 1/S transition, in cells grown in 2%, but not in 5% glucose. A non-phosphorylatable Snf1 rescues the slow growth phenotype, whereas a wild type or a phosphomimetic mutant is required to rescue growth rate and the G 1/S delay. Using either Snf1 or Swi6 as a bait, a specific interaction of Snf1 with Swi6, the regulatory subunit of MBF, was detected. In conclusion, this report describes a previously unrecognized role for Snf1 in transcriptional modulation of the G 1 to S transition, differing from the reported AMPK role in controlling the G 1/S transition in multicellular eukaryotes.
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milano, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Pessina
authors.forename: Viktoryia
authors.initials: V
authors.lastname: Tsiarentsyeva
authors.forename: Sara
authors.initials: S
authors.lastname: Busnelli
authors.forename: Marco
authors.initials: M
authors.lastname: Vanoni
authors.forename: Lilia
authors.initials: L
authors.lastname: Alberghina
authors.forename: Paola
authors.initials: P
authors.lastname: Coccetti
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D016193:G1 Phase; D005947:Glucose / Q000494:pharmacology; D009154:Mutation; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D021122:Protein Subunits / Q000378:metabolism; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: C076558:CLB5 protein, S cerevisiae; D018797:Cell Cycle Proteins; D019926:Cyclin B; D021122:Protein Subunits; C115043:SLD2 protein, S cerevisiae; C075111:SWI6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases; D005947:Glucose