Effect of peroxynitrite scavenging on endothelial cells stimulated by plasma from women with preeclampsia: a proteomic approach.
OBJECTIVE
Increased peroxynitrite formation in endothelial cells incubated with plasma from women with preeclampsia alters the expression of specific endothelial proteins.


METHODS
We used a proteomic approach to examine the effect of a peroxynitrite scavenger (FeTPPS, 5 micromol/L) on the expression of endothelial proteins in response to plasma from women with preeclampsia. After treatment, cells were subjected to two-dimensional gel electrophoresis and proteins were identified using mass spectrometry.


RESULTS
Peroxynitrite scavenging decreased the expression of structural proteins, cytokeratin, actin-gamma-1, and moesin, and the calcium-binding protein, calumenin.


CONCLUSIONS
This study has identified novel proteins that are regulated by peroxynitrite in response to plasma from women with preeclampsia.
Sankaralingam
Sowndramalingam
S
Department of Physiology, University of Alberta, Edmonton, Alberta, Canada.
Lalu
Manoj M
MM
Xu
Yi
Y
Davidge
Sandra T
ST
D000328:Adult; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D018384:Oxidative Stress; D030421:Peroxynitrous Acid / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D040901:Proteomics; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism*
D030421:Peroxynitrous Acid