Microbial products (biosurfactant and extracellular chromate reductase) of marine microorganism are the potential agents reduce the oxidative stress induced by toxic heavy metals.
The present study demonstrates hexavalent chromium reduction and trivalent chromium tolerance behavior of marine Bacillus sp., MTCC 5514 through its extracellular enzyme reductase and biosurfactants production. The isolate reduces 10-2000 mg/L of hexavalent chromium to trivalent chromium with in 24-96 h respectively and the release of extracellular chromium reductase, found responsible for the reduction. Upon reduction, the concentration of trivalent chromium in the medium found comparatively less. Experimental results reveal, biosurfactants activity found responsible for the less concentration of Cr(III). Hypothetically, trivalent chromium upon formation get entrapped in the micelle of biosurfactants, prevents microbial cells from exposure towards trivalent chromium. Thus, the chosen isolate exhibit tolerance and growth with the increasing concentration of chromium.
Gnanamani
A
A
Microbiology Division, Central Leather Research Institute, (CSIR, New Delhi), Adyar, Chennai 600020, Tamil Nadu, India. gnanamani3@gmail.com
Kavitha
V
V
Radhakrishnan
N
N
Suseela Rajakumar
G
G
Sekaran
G
G
Mandal
A B
AB
D001419:Bacteria / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D002857:Chromium / Q000378:metabolism; D005110:Extracellular Space / Q000187:drug effects / Q000201:enzymology*; D019216:Metals, Heavy / Q000633:toxicity*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D013501:Surface-Active Agents / Q000494:pharmacology*
D019216:Metals, Heavy; D013501:Surface-Active Agents; D002857:Chromium; C074702:chromium hexavalent ion; D010088:Oxidoreductases; C063243:chromate reductase