[Effects of costimulatory molecule VSIG4 exclusively expression in macrophage on renal interstitial fibrosis].
OBJECTIVE
To investigate the relationship between costimulatory molecule VSIG4 expression in macrophage and mice renal interstitial fibrosis.


METHODS
A total of 40 healthy male C57B6 mice (VSIG4(-/-); and VSIG4(+/+);) were divided into two groups, VSIG4(-/-); tubulointerstitial Nephritis (n=20) and VSIG4(+/+); tubulointerstitial Nephritis (n=20), mice received left urethral obstruction operation(UUO) then were killed at 7 d before operation, 3 d, 7 d and 14 d post operation for both groups.Blood SCr and BUN were detected. Renal interstitial fibrosis was measured by HE stain. The expression of TGF-beta1, MMP-2, in UUO mice(VSIG4(-/-); and VSIG4(+/+);) were detected by immunohistochemistry.


RESULTS
SCr and BUN levels in the two groups have no significantly different. TGF-beta1 was significantly increased in the VSIG4(-/-); UUO mice group in comparison with the other group, while MMP-2 was reduced.


CONCLUSIONS
The interstitial inflammatory cell infiltration and tubular lesion of VSIG4(-/-); UUO mice were increased, with the expression of TGF-beta1 increased and MMP-2 reduced. VSIG4 may play a role in inhibiting interstitial fibrosis.
Li
Yan
Y
Department of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Wang
Yi-qin
YQ
Li
Ming
M
Zhang
Ying
Y
Li
Fu-rong
FR
Wang
Zi-hua
ZH
Qi
Wei
W
Yuan
Fa-huan
FH
D000818:Animals; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009395:Nephritis, Interstitial / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011951:Receptors, Complement / Q000235:genetics / Q000502:physiology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D011951:Receptors, Complement; D053773:Transforming Growth Factor beta1; C509992:VSIG4 protein, mouse; D020778:Matrix Metalloproteinase 2