Isolation and structure determination of one new metabolite isolated from the red fermented rice of Monascus purpureus.
The n-BuOH-soluble portion of the 95% EtOH extract of red fermented rice fermented with the yellow mutant of the fungus Monascus purpureus BCRC 38113 (Monascaceae) led to the isolation of one new pyran-2-one derivative, namely peroxymonascuspyrone (1), along with nine known compounds, monasfluore A (2), monasfluore B (3), 3-epi-betulinic acid (4), 3-epi-betulinic acid acetate (5), alpha-tocospiro A (6), friedelan-3-one (7), alpha-cadinol (8), anticopalol (9), and spathulenol (10). Interestingly, this is the first report of a naturally occurring pyran-2-one skeleton isolated from Monascus sp. Their structures and relative configurations were elucidated by spectroscopic methods, including 1D- and 2D-NMR ((1)H,(1)H-COSY, HMQC, HMBC and NOESY), as well as low- and high-resolution mass spectrometric analyses. In addition, cytotoxicities against MCF-7, NCI-H460 and SF-268 cancer cell lines were measured in vitro; the results revealed that these metabolites have no cytotoxicity against the selected tumour cells.
Cheng
Ming-Jen
MJ
Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan. chengmingjen2001@yahoo.com.tw
Chen
Jih-Jung
JJ
Wu
Ming-Der
MD
Yang
Ping-Shin
PS
Yuan
Gwo-Fang
GF
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005285:Fermentation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D032741:Monascus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology
D010936:Plant Extracts