[Effect of polyethylene oxide on red blood cell velocity in rat cremaster microcirculation].
OBJECTIVE
To investigate the drag-reducing effect of polyethylene oxide (PEO) on the velocity of red blood cells in rat cremaster microcirculation.


METHODS
Blood samples were collected from 6 Wistar male rats (100-110 g) via the post-orbital venous plexus. The red blood cells were separated by centrifugation and labeled by fluorescinisothiocyate (FITC). After successful establishment of cremaster model, the labeled red blood cells were injected into the jugular vein, and the microcirculation was observed and recorded under fluorescence microscope. The hemodynamic parameters and microcirculation video was recorded every 4 min since 4 min before PEO or normal saline injection. Both PEO (10 ppm) and normal saline was injected into the same rat in random sequence at a constant rate of 3.5 ml/h for 20 min followed by observation for another 20 min. The velocity of the labeled-red blood cells was determined by IPP 6.0 software.


RESULTS
Compared with normal saline, PEO significantly increased the velocity of the red blood cells in the rat cremaster microcirculation (498.7-/+182.89 microm/s vs 773.54-/+308.27 microm/s, P=0.012). No significant changes in the heart rate and arterial blood pressure were observed during the experiment (P=0.836, P=0.420).


CONCLUSIONS
PEO at an extremely low concentration can significantly increase the velocity of the red blood cells in rat cremaster microcirculation and produces no significant impact on heart rate and arterial blood pressure.
Du
Rong-sheng
RS
Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. durongsheng2009@163.com
Zha
Dao-gang
DG
Zhou
Bing-jie
BJ
Hu
Feng
F
Ji
Li-jing
LJ
Wu
Jue-fei
JF
Bin
Jian-ping
JP
Liu
Yi-li
YL
D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D008297:Male; D008833:Microcirculation / Q000187:drug effects* / Q000502:physiology; D009130:Muscle, Smooth / Q000098:blood supply*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis
D011092:Polyethylene Glycols