Activities of glutamine synthetase, glutaminase and arginase in kainic acid lesioned rat brain corpus striatum.
Intrastriatal kainic acid (2 ?g/?l) administration gave rise to significant increase in activities of glutamine synthetase and arginase along with a significant decrease in the activity of glutaminase in the lesioned striatal tissue 7 days after the administration of kainic acid. The increase in the activity of glutamine synthetase was attributed to the gliosis occurring in such lesions. The decrease in the activity of glutaminase was thought to be due to the loss of GABAergic neurons. The increase in arginase activity might be occurring in glial cells or in nerve endings. Although the earlier results indicated a low specific activity of arginase in glial cells, the observed increase in its activity might be partly due to its increase in proliferating glial cells, liberating ornithine for the formation of polyamines. However, it was also thought that a substantial increase may be occurring in the arginase present in the intact glutamatergic (corticostriate pathway) nerve endings, since it was earlier found that the synaptosomes of the rat brain had appreciably high activity of arginase. These results were discussed in relation to the probable roles of arginine and glutamine as the precursors for neurotransmitter pools of glutamate in striatum.
Nadiger
H A
HA
Department of Biochemistry, M. S. Ramaiah Medical College, Gokula Extension Post, Bangalore 560 054, India.
Rani Marcus
S
S
Chandrakala
M V
MV
Sadasivudu
B
B