[Comparison on HPLC analysis of aerial part of Bupleurums from different germplasm resources].
OBJECTIVE
To investigate ingredient differences of the aerial part of Bupleurums from different germplasm resources by HPLC.


METHODS
Twenty-one different germplasm resources were sown and cultured at the same conditions and the aerial parts of the biennial plants were analyzed by HPLC.


RESULTS
The chromatograms were established and the data were analyzed by Hierarchical Cluster Analysis to compare the similarity of the samples. The results showed that ingredients from various germplasm resources were significantly different, which indicated the germplasm diversity of Bupleurum.


CONCLUSIONS
The method can be used for comparison of the ingredient differences and quality control, the results provide important references for classification of Bupleurum species.
Geng
Maolin
M
Jiangsu Center for Research and Development of Medicinal Plants, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China. gmaolin1018@163.com
Song
Pingping
P
Xu
Zenglai
Z
Wang
Nianhe
N
D029972:Bupleurum / Q000737:chemistry* / Q000145:classification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D035261:Plant Components, Aerial / Q000737:chemistry*