Nuclear factor kappa B pathway down-regulates aquaporin 5 in the nasal mucosa of rats with allergic rhinitis.
Nuclear factor kappa B (NF-κB) induces gene transcription by binding CREB-binding protein (CBP). The aim of the study was to detect the mechanisms by which NF-κB pathway regulated aquaporin 5 (AQP5) in the nasal mucosa of rats with allergic rhinitis (AR). Rats were divided into control (group C), model (group M), low-dose proline dithiocarbamate (PDTC) (group L) and high-dose PDTC (group H) groups. AR model was established by the sensitization with ovalbumin, then groups L and H were treated with PDTC (50 or 100 mg/kg/day) for 5 days. AQP5, interleukin-1β, NF-κBp65 and phosphorylated cAMP-response element binding protein (p-CREB) were detected by immunohistochemistry, Western blotting or real-time PCR. AQP5 expression in group M was lower than in group C, but in groups L and H it increased. NF-κBp65 expression in group M was higher than group C, but in groups L and H it reduced. p-CREB expression in group M was lower than group C, but in groups L and H it increased. Interleukin-1β gene level in group M was higher than group C, but in groups L and H it was lower. These data show that the NF-κB pathway could down-regulate AQP5 by interleukin-1β which inhibited CREB phosphorylation or by NF-κBp65 which competitively bound CBP.
Wang
Weiwei
W
Fujian Medical University, No. 88, Jiaotong Road, Fuzhou, 350004, Fujian, China.
Zheng
Ming
M
D000704:Analysis of Variance; D000818:Animals; D051402:Aquaporin 5 / Q000378:metabolism*; D015153:Blotting, Western; D050882:CREB-Binding Protein / Q000378:metabolism; D015536:Down-Regulation; D007124:Immunoenzyme Techniques; D053583:Interleukin-1beta / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D009297:Nasal Mucosa / Q000378:metabolism*; D010047:Ovalbumin; D011392:Proline / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012221:Rhinitis, Allergic, Perennial / Q000378:metabolism*; D013859:Thiocarbamates / Q000494:pharmacology
D051402:Aquaporin 5; D053583:Interleukin-1beta; D016328:NF-kappa B; D013859:Thiocarbamates; C066229:prolinedithiocarbamate; D010047:Ovalbumin; D011392:Proline; D050882:CREB-Binding Protein