Real-time reaction monitoring by probe electrospray ionization mass spectrometry.
Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study, PESI-MS was used to monitor some biological and chemical reactions in real-time, such as acid-induced protein denaturation, hydrogen/deuterium exchange (HDX) of peptides, and Schiff base formation. By using PESI-MS, time-resolved mass spectra and ion chromatograms can be obtained reproducibly. Real-time PESI-MS monitoring can give direct and detailed information on each chemical species taking part in reactions, and this is valuable for a better understanding of the whole reaction process and for the optimization of reaction parameters. PESI-MS can be considered as a potential tool for real-time reaction monitoring due to its simplicity in instrumental setup, direct sampling with minimum sample preparation and low sample consumption.
Yu
Zhan
Z
Clean Energy Research Center, University of Yamanashi, Kofu, Japan.
Chen
Lee Chuin
LC
Erra-Balsells
Rosa
R
Nonami
Hiroshi
H
Hiraoka
Kenzo
K
D055598:Chemical Phenomena; D003199:Computer Systems; D006801:Humans; D015394:Molecular Structure; D009211:Myoglobin / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011489:Protein Denaturation; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D009211:Myoglobin; D010455:Peptides