title: Arabidopsis PIS1 encodes the ABCG37 transporter of auxinic compounds including the auxin precursor indole-3-butyric acid.
abstract: Differential distribution of the plant hormone auxin within tissues mediates a variety of developmental processes. Cellular auxin levels are determined by metabolic processes including synthesis, degradation, and (de)conjugation, as well as by auxin transport across the plasma membrane. Whereas transport of free auxins such as naturally occurring indole-3-acetic acid (IAA) is well characterized, little is known about the transport of auxin precursors and metabolites. Here, we identify a mutation in the ABCG37 gene of Arabidopsis that causes the polar auxin transport inhibitor sensitive1 (pis1) phenotype manifested by hypersensitivity to auxinic compounds. ABCG37 encodes the pleiotropic drug resistance transporter that transports a range of synthetic auxinic compounds as well as the endogenous auxin precursor indole-3-butyric acid (IBA), but not free IAA. ABCG37 and its homolog ABCG36 act redundantly at outermost root plasma membranes and, unlike established IAA transporters from the PIN and ABCB families, transport IBA out of the cells. Our findings explore possible novel modes of regulating auxin homeostasis and plant development by means of directional transport of the auxin precursor IBA and presumably also other auxin metabolites.
authors.affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, Ghent University, 9052 Ghent, Belgium.
authors.forename: Kamil
authors.initials: K
authors.lastname: Ruzicka
authors.forename: Lucia C
authors.initials: LC
authors.lastname: Strader
authors.forename: Aurélien
authors.initials: A
authors.lastname: Bailly
authors.forename: Haibing
authors.initials: H
authors.lastname: Yang
authors.forename: Joshua
authors.initials: J
authors.lastname: Blakeslee
authors.forename: Lukasz
authors.initials: L
authors.lastname: Langowski
authors.forename: Eliska
authors.initials: E
authors.lastname: Nejedlá
authors.forename: Hironori
authors.initials: H
authors.lastname: Fujita
authors.forename: Hironori
authors.initials: H
authors.lastname: Itoh
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Syono
authors.forename: Jan
authors.initials: J
authors.lastname: Hejátko
authors.forename: William M
authors.initials: WM
authors.lastname: Gray
authors.forename: Enrico
authors.initials: E
authors.lastname: Martinoia
authors.forename: Markus
authors.initials: M
authors.lastname: Geisler
authors.forename: Bonnie
authors.initials: B
authors.lastname: Bartel
authors.forename: Angus S
authors.initials: AS
authors.lastname: Murphy
authors.forename: Jirí
authors.initials: J
authors.lastname: Friml
mesh_terms: D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D006706:Homeostasis; D007210:Indoleacetic Acids / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism*; D009154:Mutation; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D007211:Indoles; C515019:PDR9 protein, Arabidopsis; C014612:indolebutyric acid