title: Common sialylated glycan in Actinobacillus suis.
abstract: A sialylated oligosaccharide was identified in four representative strains of the Gram-negative swine pathogen, Actinobacillus suis. As characterized, the glycan consists of a free oligosaccharide with a N-acetyl-lactosamine-like backbone decorated with sialic acid, phosphoethanolamine (PEA) and O-acetyl units: 9-O-Ac-Neu5Ac-(2-->6)-beta-d-Galp-(1-->4)-beta-d-6-O-Ac-GlcpNAc-(1-->3)-[PEA-->6]-beta-d-Galp-(1-->3)-beta-d-GlcpNAc-(1-->2)-[9-O-Ac-Neu5Ac-(2-->6)]-beta-d-Galp-(1-->4)-beta-d-6-O-Ac-GlcpNAc-(1-->3)-[PEA-->6]-beta-d-Galp-(1-->3)-d-GlcpNAc. The ubiquitous expression of this sialylated glycan suggests that this carbohydrate may play an important role in the survival of A. suis in the host.
authors.affiliation: Department of Chemistry, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Deutschmann
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Boncheff
authors.forename: Loredana
authors.initials: L
authors.lastname: Daraban
authors.forename: Janet I
authors.initials: JI
authors.lastname: MacInnes
authors.forename: Mario A
authors.initials: MA
authors.lastname: Monteiro
mesh_terms: D000107:Acetylation; D000189:Actinobacillus Infections / Q000378:metabolism* / Q000382:microbiology; D044132:Actinobacillus suis / Q000472:pathogenicity*; D000606:Amino Sugars / Q000737:chemistry; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004983:Ethanolamines / Q000737:chemistry; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D034421:Sus scrofa; D013553:Swine Diseases / Q000378:metabolism* / Q000382:microbiology
chemical_list: D000606:Amino Sugars; D004983:Ethanolamines; D011134:Polysaccharides; C032233:lactosamine; C005448:phosphorylethanolamine; D019158:N-Acetylneuraminic Acid