Dietary exposure to low doses of bisphenol A: effects on reproduction and development in two generations of C57BL/6J mice.
The present study was conducted to examine the effects of low-dose exposure to bisphenol A on reproduction and development in two generations of mice. Pregnant female C57BL/6J mice (F(0)) were fed a diet containing low doses of bisphenol A (0, 0.33, 3.3, or 33 ppm) from gestational day 6 through postnatal day 22, and the weanlings (F(1) and F(2)) from each F(0) and F(1) dam group, respectively, were also fed these same concentrations of bisphenol A ad libitum until sacrifice. There were no treatment-related changes in body weight, body weight gain, food consumption, gestation length, or the number of live births on postnatal day 1 in F(0) dams between the control group and bisphenol A groups. Sex ratio and viability were similar in all F(1) pups. No treatment-related changes were observed in body weight, food consumption, developmental parameters, anogenital distance, or weight of any of the organs (liver, kidney, heart, spleen, thymus, testis, ovary, or uterus) in F(1) and F(2) adults in either sex. The epididymis weight was slightly higher with 0.33 and 3.3 ppm in F(1) males, but this slight increase was neither dose dependent nor seen across generations. There were no treatment-related effects of bisphenol A on cauda epididymal sperm count or sperm motility in F(1) or F(2) males. These findings indicate that dietary exposure to bisphenol A between 0.33 and 33 ppm does not adversely affect reproduction or development as assessed in two generations of mice.
Kobayashi
Kenichi
K
Division of Health Effects Research, National Institute of Occupational Safety and Health, 6-21-1 Nagao, Tama-ku, Kawasaki, Japan. kobayasi@h.jniosh.go.jp
Ohtani
Katsumi
K
Kubota
Hisayo
H
Miyagawa
Muneyuki
M
D000818:Animals; D001559:Benzhydryl Compounds; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D004435:Eating; D004822:Epididymis; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size / Q000187:drug effects; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D012098:Reproduction / Q000187:drug effects*; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds