Protein expression changes in human monocytic THP-1 cells treated with lipoteichoic acid from Lactobacillus plantarum and Staphylococcus aureus.
Lipoteichoic acid (LTA) from Staphylococcus aureus (aLTA) and from Lactobacillus plantarum LTA (pLTA) are both recognized by Toll-like receptor 2 (TLR2), but cause different stimulatory effects on the innate immune and inflammatory responses, and their underlying cellular mechanisms are unknown. In this study, comparative proteome analysis was performed using two-dimensional gel electrophoresis and mass spectrometry on protein extracts from human monocyte THP-1 cells stimulated with either aLTA or pLTA. Differentially expressed proteins might be involved in innate immunity and inflammation. Cells treated with aLTA and with pLTA showed different protein expression profiles. Of 60 identified proteins, 10 were present only in treated cells (8 in aLTA-treated only, and 2 in pLTA-treated only), 1 protein (IMPDH2) was suppressed by pLTA, and 49 were up- or down-regulated more than three-fold by aLTA- or pLTA- stimulation. Several proteins involved in immunity or inflammation, antioxidation, or RNA processing were significantly changed in expression by aLTA- or pLTA-stimulation, including cyclophilin A, HLA-B27, D-dopachrome tautomerase, Mn- SOD, hnRNP-C, PSF and KSRP. These data demonstrated that aLTA and pLTA had different effects on the protein profile of THP-1 cells. Comparison of the proteome alterations will provide candidate biomarkers for further investigation of the immunomodulatory effects of aLTA and pLTA, and the involvement of aLTA in the pathogenesis of Staphylococcus aureus sepsis.
Zeng
Ri-Zhong
RZ
School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 449-701, Korea.
Kim
Han Geun
HG
Kim
Na Ra
NR
Lee
Hae Young
HY
Jung
Bong Jun
BJ
Ko
Mi Yeon
MY
Lee
Seung Yeon
SY
Chung
Dae Kyun
DK
D015415:Biomarkers / Q000378:metabolism; D045744:Cell Line, Tumor; D021984:Cyclophilin A / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D015796:HLA-B27 Antigen / Q000378:metabolism; D006801:Humans; D007168:IMP Dehydrogenase / Q000378:metabolism; D056747:Immunomodulation; D048191:Lactiplantibacillus plantarum / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D013058:Mass Spectrometry; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020543:Proteome / Q000378:metabolism*; D018805:Sepsis / Q000276:immunology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000276:immunology*; D013682:Teichoic Acids / Q000494:pharmacology*
D015415:Biomarkers; D015796:HLA-B27 Antigen; D008070:Lipopolysaccharides; D020543:Proteome; D013682:Teichoic Acids; C009900:lipoteichoic acid; D007168:IMP Dehydrogenase; C501687:IMPDH2 protein, human; D021984:Cyclophilin A