Oxidative stress-based cytotoxicity of delphinidin and cyanidin in colon cancer cells.
Colorectal cancer is the second most frequent cause of cancer death in the western world. Although the prognosis has improved after the introduction of newer anticancer drugs, the treatment of metastatic colorectal cancer still remains a challenge due to a high percentage of drug-resistant tumor forms. We aimed at testing whether anthocyanidins exerted cytotoxicity in primary (Caco-2) and metastatic (LoVo and LoVo/ADR) colorectal cancer cell lines. Both cyanidin and delphinidin, though neither pelargonidin nor malvidin, were cytotoxic in metastatic cells only. The cell line most sensitive to anthocyanidins was the drug-resistant LoVo/ADR. There, cellular ROS accumulation, inhibition of glutathione reductase, and depletion of glutathione could be observed. This suggests that anthocyanidins may be used as sensitizing agents in metastatic colorectal cancer therapy.
Cvorovic
Jovana
J
Department of Life Sciences, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.
Tramer
Federica
F
Granzotto
Marilena
M
Candussio
Luigi
L
Decorti
Giuliana
G
Passamonti
Sabina
S
D000872:Anthocyanins / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D018938:Caco-2 Cells; D002166:Camptothecin / Q000494:pharmacology; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology / Q000556:secondary; D019008:Drug Resistance, Neoplasm; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects
D000872:Anthocyanins; D000975:Antioxidants; D016877:Oxidants; C065861:malvidin; C066957:pelargonidin; C017154:cyanidin; D005980:Glutathione Reductase; C017185:delphinidin; D005978:Glutathione; D002166:Camptothecin