title: On-chip immunoassay using surface-enhanced Raman scattering of hollow gold nanospheres.
abstract: A surface-enhanced Raman scattering (SERS)-based gradient optofluidic sensor has been developed for a fast and sensitive immunoassay. In this work, a novel microfluidic sensor with functional internal structures has been designed and fabricated. This sensor is composed of three compartments consisting of the gradient channel that serially dilutes the target marker, the injection and mixing area of antibody-conjugated hollow gold nanospheres and magnetic beads, and the trapping area of sandwich immunocomplexes using multiple solenoids. Quantitative analysis of a specific target marker is performed by analyzing its characteristic SERS signals. This SERS-based gradient optofluidic sensor can replace the set of microwells or microtubes used in manual serial dilutions that have been traditionally used in enzyme-linked immunosorbent assay (ELISA)-type assays. The limit of detection for rabbit immunoglobin (IgG) is estimated to be 1-10 ng/mL. This novel SERS-based optofluidic immunoassay system is expected to be a powerful clinical tool for the fast and sensitive medical diagnosis of a disease.
authors.affiliation: Department of Bionano Engineering, Hanyang University, Ansan 426-791, South Korea.
authors.forename: Hyangah
authors.initials: H
authors.lastname: Chon
authors.forename: Chaesung
authors.initials: C
authors.lastname: Lim
authors.forename: Seung-Mo
authors.initials: SM
authors.lastname: Ha
authors.forename: Yoomin
authors.initials: Y
authors.lastname: Ahn
authors.forename: Eun Kyu
authors.initials: EK
authors.lastname: Lee
authors.forename: Soo-Ik
authors.initials: SI
authors.lastname: Chang
authors.forename: Gi Hun
authors.initials: GH
authors.lastname: Seong
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
mesh_terms: D000818:Animals; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis*; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D054118:Nanospheres / Q000737:chemistry*; D011817:Rabbits; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods
chemical_list: D007074:Immunoglobulin G; D006046:Gold