title: Trafficking of UL37 proteins into mitochondrion-associated membranes during permissive human cytomegalovirus infection.
abstract: Human cytomegalovirus (HCMV) UL37 proteins traffic sequentially from the endoplasmic reticulum (ER) to the mitochondria. In transiently transfected cells, UL37 proteins traffic into the mitochondrion-associated membranes (MAM), the site of contact between the ER and mitochondria. In HCMV-infected cells, the predominant UL37 exon 1 protein, pUL37x1, trafficked into the ER, the MAM, and the mitochondria. Surprisingly, a component of the MAM calcium signaling junction complex, cytosolic Grp75, was increasingly enriched in heavy MAM from HCMV-infected cells. These studies show the first documented case of a herpesvirus protein, HCMV pUL37x1, trafficking into the MAM during permissive infection and HCMV-induced alteration of the MAM protein composition.
authors.affiliation: Center for Cancer and Immunology Research, Children's Research Institute, Children's National Medical Center, Washington, DC 200101, USA.
authors.forename: Petros
authors.initials: P
authors.lastname: Bozidis
authors.forename: Chad D
authors.initials: CD
authors.lastname: Williamson
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Wong
authors.forename: Anamaris M
authors.initials: AM
authors.lastname: Colberg-Poley
mesh_terms: D002478:Cells, Cultured; D003586:Cytomegalovirus Infections / Q000473:pathology* / Q000821:virology*; D004721:Endoplasmic Reticulum / Q000737:chemistry; D005347:Fibroblasts / Q000821:virology; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D017874:Immediate-Early Proteins / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis; D051336:Mitochondrial Membranes / Q000737:chemistry*; D021381:Protein Transport
chemical_list: D018840:HSP70 Heat-Shock Proteins; D017874:Immediate-Early Proteins; D008565:Membrane Proteins; C420323:UL37 protein, Human herpesvirus 5; C018551:glucose-regulated proteins