Enhancement of bacteriophage λ stability using a λQ-S- mutant in the continuous culture of Escherichia coli.
In this study, we used a bacteriophage λQ⁻S⁻ mutant that increased the stability of recombinant Escherichia coli during continuous culture. The operation was conducted in two stages: the first stage was carried out to promote cell growth, and the second stage was performed for product formation. The productivity of recombinant proteins depends on the substrate concentration of the fresh medium supplied to the second stage (S₃) and dilution rate of the second stage (D₂). With the optimal value of S₃ and D₂, the first and second stages were stably maintained for 170 and 80 h, respectively. To further improve this process, a three-stage continuous process was conducted with an additional induction stage between the growth and production stages. Compared with the two-stage operation, the stable production period was extended by 1.7 fold, and the recombinant protein production increased by 1.3 fold.
Oh
Jeong Seok
JS
School of Chemical and Biological Engineering, Seoul National University, San 56-1, Shilim-Dong, Kwanak-Gu, Seoul, Korea.
Choi
Shin Sik
SS
Park
Tai Hyun
TH
D010582:Bacteriophage lambda / Q000235:genetics* / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000821:virology; D005818:Genetic Engineering / Q000379:methods; D008957:Models, Genetic; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
D004247:DNA; D001616:beta-Galactosidase