Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors.
Catechins (bioactive polyphenols in green tea) are known to exhibit potent anti-inflammatory properties. However, the anti-inflammatory effects of catechins on inflamed dental pulp tissue are not known. In this study, we investigated the effect of epigallocatechin-3-gallate (EGCG) and epicatechin gallate (ECG), the major components of green tea catechins, on the expression of pro-inflammatory cytokines and adhesion molecules in human dental pulp cells stimulated with bacteria-derived factors such as lipopolysaccharide (LPS) and peptidoglycan (PG). The expression of interleukin (IL)-6 and of IL-8 was examined using the reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assays. The expression of intercellular adhesion molecule-1 (ICAM-1) and of vascular cell adhesion molecule-1 (VCAM-1) on dental pulp cells was analyzed using flow cytometry. The presence of EGCG and ECG significantly reduced, in a concentration-dependent manner, the expression of IL-6 and IL-8 in dental pulp cells exposed to LPS or PG. Increased expression of ICAM-1 and VCAM-1 on the dental pulp cells in response to bacterial components was also decreased by treatment with EGCG and ECG. These findings suggest that green tea catechins may prevent the exacerbation of pulpitis.
Nakanishi
Tadashi
T
Department of Conservative Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan. tadashi@dent.tokushima-u.ac.jp
Mukai
Kayo
K
Yumoto
Hiromichi
H
Hirao
Kouji
K
Hosokawa
Yoshitaka
Y
Matsuo
Takashi
T
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects / Q000382:microbiology*; D004926:Escherichia coli; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010457:Peptidoglycan / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013211:Staphylococcus aureus; D013997:Time Factors; D019010:Vascular Cell Adhesion Molecule-1 / Q000187:drug effects; D000093242:Interleukin-6 Inhibitors
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D002392:Catechin; D018799:Intercellular Adhesion Molecule-1; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D019010:Vascular Cell Adhesion Molecule-1; D000093242:Interleukin-6 Inhibitors; C062669:epicatechin gallate; C045651:epigallocatechin gallate