Analyses for phosphatidylcholine hydroperoxides by LC/MS.
A new liquid chromatography mass spectrometry (LC/MS) method has been developed for the qualitative and quantitative analyses of phosphatidylcholine hydroperoxides (PC-OOH) in human plasma using a synthetic hydroperoxide (1-stearoyl-2-erucoyl-PC monohydroperoxide, PC 18:0/22:1-OOH) as an internal standard. 1-Stearoyl-2-linoleoyl-PC monohydroperoxide (PC 18:0/18:2-OOH) was identified in plasma by LC/MS by comparison with an authentic standard. The calibration curves obtained for 1-palmitoyl-2-linoleoyl-PC monohydroperoxide, PC 16:0/18:2-OOH and PC 18:0/18:2-OOH were linear throughout the calibration range (0.1-1.0 pmol). The limit of detection (LOD) (S/N=3:1) was 0.01 pmol, and the limit of quantification (LOQ) (S/N=6:1) was 0.1 pmol for both PC 16:0/18:2-OOH and PC 18:0/18:2-OOH. Plasma concentrations of PC 16:0/18:2-OOH and PC 18:0/18:2-OOH were 89 and 32 nM, respectively, in a healthy volunteer.
Hui
Shu-Ping
SP
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan. keino@hoku-iryo-u.ac.jp
Chiba
Hitoshi
H
Jin
Shigeki
S
Nagasaka
Hironori
H
Kurosawa
Takao
T
D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000097:blood*
D010713:Phosphatidylcholines; C057590:phosphatidylcholine hydroperoxide