Response surface optimization of the critical medium components for the production of alpha-galactosidase from Aspergillus parasiticus MTCC-2796.
Response surface methodology was used to evaluate the effect of main variables such as concentration of galactose, yeast extract and wheat bran on alpha-galactosidase production from Aspergillus parasiticus MTCC-2796 under submerged fermentation conditions. A full factorial Central Composite Design was applied to study these main factors that affected alpha-galactosidase production. The experimental results showed that the optimum concentration of galactose, yeast extract and wheat bran were 1.5 %, 0.06 % and 1.5 %, respectively. This method was efficient as only 20 experiments were necessary to asses these conditions, and model adequacy was very satisfactory as the coefficient of determination was 0.9921.
Shivam
K
K
Department of Biotechnology, D. D. U. Gorakhpur University, Gorakhpur, India. k.shivam@rediffmail.com
Mishra
S K
SK
D001230:Aspergillus / Q000201:enzymology*; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation / Q000502:physiology*; D013499:Surface Properties; D000519:alpha-Galactosidase / Q000096:biosynthesis*
D003470:Culture Media; D000519:alpha-Galactosidase