title: [(35)S]TBPS binding to purified benzodiazepine-GABA-ionophore receptor complex: the effect of GABA on the modulation exerted by benzodiazepine site ligands.
abstract: Benzodiazepine GABA-ionophore receptor complex ligands showed persistent modulation of the chloride ionophore, labeled by [(35)S]TBPS, even after receptor complex extensive purification. GABA caused inhibition of [(35)S]TBPS binding, while benzodiazepine agonists increased and benzodiazepine inverse agonists decreased the specific [(35)S]TBPS binding to the purified receptor. When GABA binding sites were occupied by the neurotransmitter benzodiazepine receptor agonists and antagonists reversed their effects clonazepam in fact inhibited and ?-carboline ethyl ester increased [(35)S]TBPS binding in the presence of GABA. The antagonist flumazenil showed no effect both in the presence or absence of GABA.
authors.affiliation: Istituto di Chimica Biologica, Universita' di Parma, 43100 Parma, Italy; Istituto Policattedra di Discipline Biologiche, Universita' di Pisa, 56100 Pisa, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Martini
authors.forename: R
authors.initials: R
authors.lastname: Pacini
authors.forename: G
authors.initials: G
authors.lastname: Giannaccini
authors.forename: A
authors.initials: A
authors.lastname: Lucacchini