title: Urinary bladder smooth muscle regeneration utilizing bone marrow derived mesenchymal stem cell seeded elastomeric poly(1,8-octanediol-co-citrate) based thin films.
abstract: Bladder regeneration studies have yielded inconclusive results possibly due to the use of unfavorable cells and primitive scaffold design. We hypothesized that human mesenchymal stem cells seeded onto poly(1,8-octanediol-co-citrate) elastomeric thin films would provide a suitable milieu for partial bladder regeneration. POCfs were created by reacting citric acid with 1,8-octanediol and seeded on opposing faces with human MSCs and urothelial cells; normal bladder smooth muscle cells and UCs, or unseeded POCfs. Partial cystectomized nude rats were augmented with the aforementioned POCfs, enveloped with omentum and sacrificed at 4 and 10 weeks. Isolated bladders were subjected to Trichrome and anti-human gamma-tubulin, calponin, caldesmon, smooth muscle gamma-actin, and elastin stainings. Mechanical testing of POCfs revealed a Young's modulus of 138 kPa with elongation 137% its initial length without permanent deformation demonstrating its high uniaxial elastic potential. Trichrome and immunofluorescent staining of MSC/UC POCf augmented bladders exhibited typical bladder architecture with muscle bundle formation and the expression and retention of bladder smooth muscle contractile proteins of human derivation. Quantitative morphometry of MSC/UC samples revealed muscle/collagen ratios approximately 1.75x greater than SMC/UC controls at 10 weeks. Data demonstrate MSC seeded POCfs support partial regeneration of bladder tissue in vivo.
authors.affiliation: Children's Memorial Hospital Chicago, Division of Pediatric Urology, 2300 Children's Plaza, Chicago, IL 60614, USA. arun-sharma@northwestern.edu
authors.forename: Arun K
authors.initials: AK
authors.lastname: Sharma
authors.forename: Partha V
authors.initials: PV
authors.lastname: Hota
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Matoka
authors.forename: Natalie J
authors.initials: NJ
authors.lastname: Fuller
authors.forename: Danny
authors.initials: D
authors.lastname: Jandali
authors.forename: Hatim
authors.initials: H
authors.lastname: Thaker
authors.forename: Guillermo A
authors.initials: GA
authors.lastname: Ameer
authors.forename: Earl Y
authors.initials: EY
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001391:Azo Compounds; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D055119:Elastic Modulus / Q000187:drug effects; D020724:Elastomers / Q000494:pharmacology*; D004801:Eosine Yellowish-(YS); D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008739:Methyl Green; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011108:Polymers / Q000494:pharmacology*; D051381:Rats; D011923:Rats, Nude; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D013194:Staining and Labeling; D054457:Tissue Scaffolds / Q000737:chemistry; D001743:Urinary Bladder / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
chemical_list: D001391:Azo Compounds; D002951:Citrates; D020724:Elastomers; D011108:Polymers; C513817:poly(1,8-octanediol citrate); C017768:trichrome stain; D019343:Citric Acid; D008739:Methyl Green; D003094:Collagen; D004801:Eosine Yellowish-(YS)