New sludge pretreatment method to improve methane production in waste activated sludge digestion.
During two-phase sludge anaerobic digestion, sludge is usually hydrolyzed and acidified in the first phase, then methane is produced in the second stage. To get more methane from sludge, most studies in literature focused on the increase of sludge hydrolysis. In this paper a different sludge pretreatment method, i.e., pretreating sludge at pH 10 for 8 d is reported, by which both waste activated sludge hydrolysis and acidification were increased, and the methane production was significantly improved. First, the effect of different sludge pretreatment methods on methane yield was compared. The pH 10 pretreated sludge showed the highest accumulative methane yield (398 mL per g of volatile suspended solids), which was 4.4-, 3.5-, 3.1-, and 2.3-fold of the blank (unpretreated), ultrasonic, thermal, and thermal-alkaline pretreated sludge, respectively. Nevertheless, its total time involved in the first (hydrolysis and acidification) and second (methanogenesis) stages was 17 (8 + 9) d, which was almost the same as other pretreatments. Then, the mechanisms for pH 10 pretreatment significantly improving methane yield were investigated. It was found that pretreating sludge at pH 10 caused the greatest sludge hydrolysis, acidification, soluble C:N and C:P ratios, and Fe(3+) concentration with a suitable short-chain fatty acids composition in the first stage, which resulted in the highest microorganism activity (ATP) and methane production in the second phase. Further investigation on the second phase microorganisms with fluorescence in situ hybridization (FISH) and scanning electron microscopy (SEM) indicated that there were much greater active methanogenesis Archaea when methane was produced with the pH 10 pretreated sludge, and the predominant morphology of the microcolonies suggest a shift to Methanosarcina sp. like.
Zhang
Dong
D
State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Chen
Yinguang
Y
Zhao
Yuxiao
Y
Zhu
Xiaoyu
X
D000693:Anaerobiosis / Q000187:drug effects; D001419:Bacteria / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D001673:Biodegradation, Environmental / Q000187:drug effects; D002244:Carbon / Q000032:analysis; D005227:Fatty Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D017404:In Situ Hybridization, Fluorescence; D007501:Iron / Q000494:pharmacology; D008697:Methane / Q000378:metabolism*; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D012722:Sewage / Q000032:analysis* / Q000382:microbiology; D013997:Time Factors; D014131:Trace Elements / Q000032:analysis; D014865:Waste Disposal, Fluid / Q000379:methods*; D018508:Water Purification / Q000379:methods*
D005227:Fatty Acids; D012722:Sewage; D014131:Trace Elements; D010758:Phosphorus; D002244:Carbon; D007501:Iron; D009584:Nitrogen; D008697:Methane