Visible fluorescence detection of type III protein secretion from bacterial pathogens.
Type III protein secretion is essential for many gram-negative bacterial infections of host cells and an attractive target for new antibacterial drugs. Here, we describe a bacterial protein effector-carboxypeptidase G2 (CPG2) reporter system for fluorescence and visible detection of type III protein secretion in Salmonella typhimurium. This system provides a general method for measuring protein expression and secretion as well as a high-throughput and quantitative assay for analyzing type III protein secretion inhibitors.
Yount
Jacob S
JS
Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065, USA.
Tsou
Lun K
LK
Dossa
Paul D
PD
Kullas
Amy L
AL
van der Velden
Adrianus W M
AW
Hang
Howard C
HC
D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D003116:Color; D006367:HeLa Cells; D006801:Humans; D019779:Salmonella enterica; D013050:Spectrometry, Fluorescence / Q000379:methods*; D011623:gamma-Glutamyl Hydrolase / Q000378:metabolism
D001426:Bacterial Proteins; D011623:gamma-Glutamyl Hydrolase