title: The effect of intracellular calcium oscillations on fluid secretion in airway epithelium.
abstract: Airway epithelium has been shown to elicit fluid secretion after a rise in intracellular calcium. This rise in intracellular calcium has been shown to display complex oscillations in many species after the binding of particular agonists to extracellular receptors. Fluid secreted by the airway epithelium is used to maintain the depth of the periciliary liquid (PCL) above the apical membrane of the epithelial cells lining the bronchial airways. Previous mathematical models have been published which separately consider the electrophysiology involved in regulating periciliary liquid depth, and the transmission of intracellular calcium waves in airway epithelial tissue. In this paper we present a mathematical model that combines these previous models and allows the effect of oscillations in intracellular calcium on fluid secretion by airway epithelial cells to be investigated. We show that an oscillatory calcium response produces different fluid secretion properties to that elicited by a tonic rise in intracellular calcium. These differences are shown to be due to saturation of the Ca(2+) activated ion channels.
authors.affiliation: Auckland Bioengineering Institute, Level 6, 70 Symond St, Auckland, New Zealand.
authors.forename: N J
authors.initials: NJ
authors.lastname: Warren
authors.forename: M H
authors.initials: MH
authors.lastname: Tawhai
authors.forename: E J
authors.initials: EJ
authors.lastname: Crampin
mesh_terms: D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D003198:Computer Simulation; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism*; D008954:Models, Biological; D020545:Respiratory Mucosa / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D002118:Calcium