title: [Construction of eukaryotic expression vector of EGFRi-IL-24 recombinant gene].
abstract: The epithelial growth factor receptor interference (EGFRi) was obtained by synthetic primers. Overlapping PCR was used to produce EGFRi-IL-24 fusion gene, which is linked by Gly4Ser3. After sequence analysis, EGFRi-IL-24 was cloned into expression vector pPIC9k; EGFRi-IL-24/pPIC9k was linearized with SacI,and then transformed to electroporated pastoris GS115. Subsequently, positive clone was selected by G418 and PCR, and its phenotype was determined by SDS-PAGE and MTT assay. The results demonstrated that EGFRi-IL-24 protein was expressed and shown to have the potential for use in researches of its biological function and in clinical application.
authors.affiliation: Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Jianling
authors.initials: J
authors.lastname: Wang
authors.forename: Xinying
authors.initials: X
authors.lastname: Fan
authors.forename: Leyuan
authors.initials: L
authors.lastname: Bao
authors.forename: Lianxiang
authors.initials: L
authors.lastname: Du
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D000099180:Interleukin-24
chemical_list: D000970:Antineoplastic Agents; D066246:ErbB Receptors; D007378:Interleukins; D011993:Recombinant Fusion Proteins; D000099180:Interleukin-24