title: Homologous RBC-derived vesicles as ultrasmall carriers of iron oxide for magnetic resonance imaging of stem cells.
abstract: Ultrasmall superparamagnetic iron oxide (USPIO) particles are very useful for cellular magnetic resonance imaging (MRI), which plays a key role in developing successful stem cell therapies. However, their low intracellular labeling efficiency, and biosafety concerns associated with their use, have limited their potential usage. In this study we develop a novel system composed of RBC-derived vesicles (RDVs) for efficient delivery of USPIO particles into human bone marrow mesenchymal stem cells (MSCs) for cellular MRI in vitro and in vivo. RDVs are highly biosafe to their autologous MSCs as manifested by cell viability, differentiation, and gene microarray assays. The data demonstrate the potential of RDVs as intracellular delivery vehicles for biomedical applications.
authors.affiliation: Center for Nanomedicine Research, National Health Research Institutes, Zhunan, Miaoli County, Taiwan.
authors.forename: Microsugar
authors.initials: M
authors.lastname: Chang
authors.forename: Jong-Kai
authors.initials: JK
authors.lastname: Hsiao
authors.forename: Ming
authors.initials: M
authors.lastname: Yao
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Chien
authors.forename: Szu-Chun
authors.initials: SC
authors.lastname: Hsu
authors.forename: Bor-Sheng
authors.initials: BS
authors.lastname: Ko
authors.forename: Shin-Tai
authors.initials: ST
authors.lastname: Chen
authors.forename: Hon-Man
authors.initials: HM
authors.lastname: Liu
authors.forename: Yao-Chang
authors.initials: YC
authors.lastname: Chen
authors.forename: Chung-Shi
authors.initials: CS
authors.lastname: Yang
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Huang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology; D005290:Ferric Compounds / Q000008:administration & dosage*; D015870:Gene Expression; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide