title: [Combined effect of doxorubicine and dehydroepiandrosterone on proliferation and oxidative stress in Saccharomyces cerevisiae cells].
abstract: Results of a comparative study of the influence of doxorubicine (DOX) and dehydroepiandrosterone (DHEA) on cell proliferation and oxidative stress in Saccharomyces cerevisiae cells are presented. Three treatment schedules were assessed--DOX only, DHEA only, and DOX simultaneously with DHEA--in examining cell proliferation, measuring the content of glutathione, and evaluating the expression of ribonucleotide reductase in the test cells. The results indicate that the separate treatment with DOX or DHEA stimulates the expression of ribonucleotide reductase and leads to a decrease in the rate of cell proliferation. DHEA produces a dose-dependent decrease in the content of a reduced form of glutathione in cells, whereas the concentration of the oxidized form remains unchanged. In contrast, the treatment with DOX increased the concentrations of both forms of glutathione. The simultaneous treatment of cells by DOX and DHEA increased the accumulation of intracellular glutathione and decreased the total antiproliferative effect.
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Saenko
authors.forename: S M
authors.initials: SM
authors.lastname: Napalkova
authors.forename: E Sh
authors.initials: ESh
authors.lastname: Enikeev
authors.forename: E V
authors.initials: EV
authors.lastname: Rastorgueva
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D012264:Ribonucleotide Reductases / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis
chemical_list: D000276:Adjuvants, Immunologic; D000903:Antibiotics, Antineoplastic; D029701:Saccharomyces cerevisiae Proteins; D003687:Dehydroepiandrosterone; D004317:Doxorubicin; D012264:Ribonucleotide Reductases; D005978:Glutathione