Molecular and virological analyses of dengue virus responsible for dengue outbreak in East Timor in 2005.
A severe dengue outbreak occurred in East Timor in 2005. The dengue virus genome was detected by TaqMan RT-PCR in 40 serum samples, as follows: dengue virus type-3 (DENV-3) in 37 samples, DENV-2 in 2 samples, and DENV-1 in one sample. One DENV-1 genome, one DENV-2 genome, and 5 DENV-3 genomes were sequenced, and these specimens were aligned with the previously determined envelope (E) gene sequences. The DENV-1 strain belonged to genotype IV and was close to those previously isolated in Indonesia and Australia. The DENV-2 strain belonged to genotype I and was close to those previously isolated in Indonesia, Australia, the Far East, and India in 1993-2001. The DENV-3 strain belonged to genotype I and was close to those previously isolated in Indonesia. The results indicate that the dengue outbreak was caused mainly by DENV-3, with DENV-1 and DENV-2 as minor serotypes, and suggest that these strains of 3 serotypes of DENV entered East Timor from neighboring countries, co-circulated, and caused the dengue outbreak in 2005.
Ito
Mikako
M
Department of Virology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.
Takasaki
Tomohiko
T
Kotaki
Akira
A
Tajima
Shigeru
S
Yuwono
Djoko
D
Rimal
Hem Sagar
HS
dos Santos
Filipe
F
de Jesus
Maria Dolores
MD
Lina
Bernadina Bano
BB
Tsuda
Yoshio
Y
Lim
Chang-Kweng
CK
Nerome
Reiko
R
Calerés
Antonio
A
Shindo
Nahoko
N
Drager
Renu Dayal
RD
Andjaparidze
Alex
A
Kurane
Ichiro
I
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003715:Dengue / Q000453:epidemiology* / Q000821:virology; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D010802:Phylogeny; D049648:Timor-Leste / Q000453:epidemiology