Application of a luminescent bacterial biosensor for the detection of tetracyclines in routine analysis of poultry muscle samples.
Tetracyclines are extensively used in veterinary medicine. For the detection of tetracycline residues in animal products, a broad array of methods is available. Luminescent bacterial biosensors represent an attractive inexpensive, simple and fast method for screening large numbers of samples. A previously developed cell-biosensor method was subjected to an evaluation study using over 300 routine poultry samples and the results were compared with a microbial inhibition test. The cell-biosensor assay yielded many more suspect samples, 10.2% versus 2% with the inhibition test, which all could be confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Only one sample contained a concentration above the maximum residue limit (MRL) of 100 microg kg(-1), while residue levels in most of the suspect samples were very low (<10 microg kg(-1)). The method appeared to be specific and robust. Using an experimental set-up comprising the analysis of a series of three sample dilutions allowed an appropriate cut-off for confirmatory analysis, limiting the number of samples and requiring further analysis to a minimum.
Pikkemaat
M G
MG
RIKILT-Institute of Food Safety, Wageningen University and Research Centre, NL-6700 AE Wageningen, the Netherlands. mariel.pikkemaat@wur.nl
Rapallini
M L B A
ML
Karp
M T
MT
Elferink
J W A
JW
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000191:economics; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000592:standards; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005062:European Union; D005506:Food Contamination; D005513:Food Inspection / Q000191:economics / Q000379:methods / Q000592:standards; D057230:Limit of Detection; D049453:Luciferases, Bacterial / Q000235:genetics / Q000378:metabolism; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D009876:Operon / Q000187:drug effects / Q000235:genetics; D011200:Poultry; D012097:Repressor Proteins / Q000378:metabolism; D015203:Reproducibility of Results; D013754:Tetracyclines / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors; D019155:Veterinary Drugs / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
D000900:Anti-Bacterial Agents; D012097:Repressor Proteins; D013754:Tetracyclines; D019155:Veterinary Drugs; D049453:Luciferases, Bacterial