Photoallergenic potential testing by online irradiation and HPLC.
A simple screening method was developed for testing the photoallergenic potential of drugs. The photolysis of the drugs under UV-A irradiation was investigated in aqueous buffer solution alone and in combination with a model dipeptide. As an useful tool for this, high performance liquid chromatography (HPLC) based on an automated column-switching system with aqueous online UV-A irradiation was used. For testing the system, hydrochlorothiazide (HC) with well-known photoallergenic properties was used as a drug and alanyltryptophane (ALATRP) as a model for proteins. Promethazine (PMZ) as a second drug was investigated by the developed screening system. The screening was performed at pH 3 and pH 7. Both cleavage of the peptide binding and formation of new products could indicate a photoallergenic potential.
Schröder
S
S
Department of Pharmacy, Free University of Berlin, Germany.
Surmann
P
P
D018926:Anti-Allergic Agents / Q000009:adverse effects; D002851:Chromatography, High Pressure Liquid; D004151:Dipeptides / Q000737:chemistry; D004232:Diuretics / Q000009:adverse effects; D004353:Drug Evaluation, Preclinical; D064420:Drug-Related Side Effects and Adverse Reactions; D006852:Hydrochlorothiazide / Q000009:adverse effects; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009862:Online Systems; D010782:Photolysis; D010787:Photosensitivity Disorders / Q000139:chemically induced*; D011398:Promethazine / Q000009:adverse effects; D011485:Protein Binding; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
D018926:Anti-Allergic Agents; D004151:Dipeptides; D004232:Diuretics; D006852:Hydrochlorothiazide; D011398:Promethazine