Construction of 4"-isovalerylspiramycin-I-producing strain by in-frame partial deletion of 3-O-acyltransferase gene in Streptomyces spiramyceticus WSJ-1, the bitespiramycin producer.
Bitespiramycin (BT), a multi-component antibiotic consisted mainly of 4"-isovalerylspiramycin I, II and III, is produced by Streptomyces spiramyceticus WSJ-1, a recombinant spiramycin-production strain that harbored the 4"-O-acyltransferase gene (ist) from Streptomyces mycarofaciens 1748, which could isovalerylate the 4"-OH of spiramycin. To eliminate the production of components 4"-isovalerylspiramycin II and III, therefore reducing the component complexity of BT, inactivation of the sspA gene, which encodes the 3-O-acyltransferase responsible for the acylation of spiramycin I to spiramycin II and III, was performed in Streptomyces spiramyceticus WSJ-1, by in-frame partial deletion. The resulting strain, Streptomyces spiramyceticus WSJ-2, is a 4"-isovalerylspiramycin-I-producing strain as expected.
Ma
Chunyan
C
Key Lab of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, CAMS & PUMC, 100050, Beijing, China.
Zhou
Hongxia
H
Li
Jingyan
J
Dai
Jianlu
J
He
Weiqing
W
Wang
Hongyuan
H
Wu
Linzhuan
L
Wang
Yiguang
Y
D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism*; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D015572:Spiramycin / Q000031:analogs & derivatives* / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
C503764:bitespiramycin; D015572:Spiramycin; D000217:Acyltransferases; C423662:carrimycin