title: Evaluation of HLA-G5 plasmatic levels during pregnancy and relationship with the 14-bp polymorphism.
abstract: OBJECTIVE
  Plasmatic HLA-G levels increase during pregnancy, but the contribution of each different isoform has not been elucidated yet.
  
  
  METHODS
  HLA-G5 was analyzed by ELISA in 19 controls, 79 women in the first 8 weeks of pregnancy and in nine women monthly until delivery. Genotyping for the 14-bp polymorphism was performed by PCR amplification of exon 8.
  
  
  RESULTS
  HLA-G5 was detected in plasma from 80% of pregnant women. The levels did not change during pregnancy, and there were no differences compared to control non-pregnant women. There was a high interindividual variation that was maintained throughout the pregnancy. The presence of +14-bp allele was associated with HLA-G5 positivity. Pregnant women who were heterozygotic to 14-bp polymorphism had significantly higher levels of HLA-G5 compared to -14 bp/-14-bp homozygotic.
  
  
  CONCLUSIONS
  Plasmatic HLA-G5 levels do not change during pregnancy and its concentration depends on 14-bp polymorphism.
authors.affiliation: Department of Biochemistry, University Clinic of Navarra, Pamplona, Spain. agonzaleh@unav.es
authors.forename: Alvaro
authors.initials: A
authors.lastname: Gonzalez
authors.forename: Estibaliz
authors.initials: E
authors.lastname: Alegre
authors.forename: Maria I
authors.initials: MI
authors.lastname: Torres
authors.forename: Angel
authors.initials: A
authors.lastname: Díaz-Lagares
authors.forename: Pedro
authors.initials: P
authors.lastname: Lorite
authors.forename: Teresa
authors.initials: T
authors.lastname: Palomeque
authors.forename: Ainhoa
authors.initials: A
authors.lastname: Arroyo
mesh_terms: D000328:Adult; D015153:Blotting, Western; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D014644:Genetic Variation; D006680:HLA Antigens / Q000097:blood* / Q000235:genetics; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000097:blood* / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011247:Pregnancy / Q000097:blood* / Q000235:genetics; D020033:Protein Isoforms; D018709:Statistics, Nonparametric
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D020033:Protein Isoforms; D004247:DNA