[Construction of conjugal transfer system of Streptomyces venezuelae var. Qinlingensis and the expression of vitreoscilla hemoglobin].
OBJECTIVE
To Construct of conjugal transfer system of Streptomyces venezuelae var. Qinlingensis and express of vitreoscilla hemoglobin.


METHODS
Intergeneric genetic transfer system was based upon plasmid pSET152 and pHZ1358 from Donor E. coli ET12567 (pUZ8002) to Streptomyces venezuelae var. Qinlingensis.


RESULTS
Our data showed that Intergeneric genetic transfer system was demonstrated and optimized. Integrating plasmid pJD100 containing Perm E and construction gene of vhb, constructed by SOE-PCR, was transformed to ET12567 (pUZ8002), and then transfered to Streptomyces venezuelae var. Qinlingensis by conjugation. The integrating expression of vhb gene in Streptomyces venezuelae var. Qinlingensis was verified by PCR and CO binding diference spectrum.


CONCLUSIONS
In summary, intergeneric genetic transfer system was demonstrated and optimized, and this is the first report to express vhb gene in Streptomyces venezuelae var. Qinlingensis.
Gao
Feng
F
College of Plant Protection and Shanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, China. gaofeng007xfg@163.com
Wang
Bin
B
Chen
Taichun
T
Wang
Zhijun
Z
An
Derong
D
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D015139:Blotting, Southern; D003227:Conjugation, Genetic / Q000235:genetics*; D006358:Hot Temperature; D016133:Polymerase Chain Reaction; D013171:Spores, Bacterial / Q000502:physiology; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D054793:Truncated Hemoglobins / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla