title: Molecular cloning and characterization of a broad substrate terpenoid oxidoreductase from Artemisia annua.
abstract: From Artemisia annua L., a new oxidoreductase (Red 1) was cloned, sequenced and functionally characterized. Through bioinformatics, heterologous protein expression and enzyme substrate conversion assays, the elucidation of the enzymatic capacities of Red1 was achieved. Red1 acts on monoterpenoids, and in particular functions as a menthone:neomenthol oxidoreductase. The kinetic parameter k(cat)/K(m) was determined to be 939-fold more efficient for the reduction of (-)-menthone to (+)-neomenthol than results previously reported for the menthone:neomenthol reductase from Mentha x piperita. Based on its kinetic properties, the possible use of Red1 in biological crop protection is discussed.
authors.affiliation: Department of Pharmaceutical Biology, GUIDE, University of Groningen, 9713 AV Groningen, The Netherlands.
authors.forename: Anna-Margareta
authors.initials: AM
authors.lastname: Rydén
authors.forename: Carolien
authors.initials: C
authors.lastname: Ruyter-Spira
authors.forename: Ralph
authors.initials: R
authors.lastname: Litjens
authors.forename: Shunji
authors.initials: S
authors.lastname: Takahashi
authors.forename: Wim
authors.initials: W
authors.lastname: Quax
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
authors.forename: Harro
authors.initials: H
authors.lastname: Bouwmeester
authors.forename: Oliver
authors.initials: O
authors.lastname: Kayser
mesh_terms: D000595:Amino Acid Sequence; D031172:Artemisia annua / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D019295:Computational Biology; D008401:Gas Chromatography-Mass Spectrometry; D015723:Gene Library; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D013729:Terpenes / Q000378:metabolism*
chemical_list: D013729:Terpenes; D010088:Oxidoreductases