Multiplexed detection of protein cancer markers on Au/Ag-barcoded nanorods using fluorescent-conjugated polymers.
Integration of fluorescent-conjugated polymers as detection moiety with metallic striped nanorods for multiplexed detection of clinically important cancer marker proteins in an immunoassay format was demonstrated in this report. Specifically, cationic conjugated polymers were introduced to protein complexes through electrostatic binding to negatively charged double-stranded DNA, which was tagged on detection antibodies prior to antigen recognition. The intense fluorescence emission of conjugated polymers resulted in highly sensitive detection of cancer marker proteins wherein an undiluted bovine serum sample as low as approximately 25 target molecules captured on each particle was detectable. Meanwhile, the use of polymer molecules as the detection probe did not obscure the optical pattern of underlying nanorods, i.e., the encoding capability of barcoded nanorods was preserved, which allowed simultaneous detection of three cancer marker proteins with good specificity.
Zheng
Weiming
W
Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
He
Lin
L
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D002417:Cattle; D001330:Electronic Data Processing; D005456:Fluorescent Dyes; D006046:Gold; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000032:analysis*; D011108:Polymers; D012680:Sensitivity and Specificity; D012834:Silver
D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D009363:Neoplasm Proteins; D011108:Polymers; D012834:Silver; D006046:Gold