Kinetic spectrophotometric determination of ciprofloxacin in a pharmaceutical preparation.
Two kinetic spectrophotometric methods were developed for determination of ciprofloxacin (CIP) in a pharmaceutical preparation. The methods are based on oxidation of CIP with potassium permanganate in alkaline media and measurement of the enhancement in the absorbance of manganate ion at 603 nm by spectrophotometry. The calibration graphs were constructed using the initial rate and fixed time methods. The linearity range for concentrations of CIP was found to be 4.0-20.0 microg/mL. The RSD values for intraday and interday precision were 0.05-0.50 and 0.07-0.63%, respectively. The procedures were applied successfully for determination of CIP in commercial tablets. The results compared well with those from a reference HPLC method. The proposed methods can be recommended for routine analysis of CIP in QC laboratories.
Aslan
Serap Saglik
SS
Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry, 34116 Beyazit, Istanbul, Turkey. serapsaglik@yahoo.com
Demir
Betul
B
D000890:Anti-Infective Agents / Q000032:analysis; D002138:Calibration; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002939:Ciprofloxacin / Q000032:analysis*; D007700:Kinetics; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011196:Potassium Permanganate / Q000737:chemistry; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D000890:Anti-Infective Agents; D004364:Pharmaceutical Preparations; D011196:Potassium Permanganate; D002939:Ciprofloxacin; D010100:Oxygen