title: Slime production by Staphylococcus aureus and Staphylococcus epidermidis strains isolated from patients with diabetic foot ulcers.
abstract: Slime production is a very important factor related to biofilm formation. The objective of the present study was to determine the frequency of slime production by Staphylococcus aureus and Staphylococcus epidermidis strains recovered from 50 patients with diabetic foot ulcers. Slime production was determined using the Congo red agar (CRA) method and compared with immunocytochemistry for the production of polysaccharide intercellular adhesin (PIA). Out of 55 S. aureus strains, 69% produced slime as shown by the CRA method. Of them, 84.2% also produced PIA. Of 17 CRA-negative strains, 70.6% produced PIA. Out of 20 S. epidermidis strains, 75% were CRA positive and 93.3% produced PIA. All CRA-negative S. epidermidis produced PIA. In conclusion, PIA production is a very common trait of S. aureus and S. epidermidis isolates obtained from diabetic foot ulcer patients.
authors.affiliation: Department of Surgical Research and Transplantology, Mossakowski Medical Research Center, Polish Academy of Sciences, Pawińskiego 5, 02-106 Warsaw, Poland.
authors.forename: Adrianna
authors.initials: A
authors.lastname: Podbielska
authors.forename: Hanna
authors.initials: H
authors.lastname: Galkowska
authors.forename: Ewa
authors.initials: E
authors.lastname: Stelmach
authors.forename: Grazyna
authors.initials: G
authors.lastname: Mlynarczyk
authors.forename: Waldemar L
authors.initials: WL
authors.lastname: Olszewski
mesh_terms: D000818:Animals; D018441:Biofilms; D004396:Coloring Agents / Q000378:metabolism; D003224:Congo Red / Q000378:metabolism; D017719:Diabetic Foot / Q000382:microbiology*; D006801:Humans; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D013203:Staphylococcal Infections / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000378:metabolism*; D013212:Staphylococcus epidermidis / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D004396:Coloring Agents; D011135:Polysaccharides, Bacterial; C113579:polysaccharide intercellular adhesin; D003224:Congo Red