Analyzing autophagy in zebrafish.
The transparency, external development and simple drug administration of zebrafish embryos makes them a useful model for studying autophagy during embryonic development in vivo. Cloning of zebrafish lc3 and generation of a transgenic GFP-Lc3 fish line provide excellent tools to monitor autophagy in this organism. ( 1)  This protocol discusses several convenient autophagy assays in zebrafish, including immunoblotting of Lc3 lipidation, microscopy imaging of GFP-Lc3 and lysosomal staining.
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Congcong
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Life Sciences Institute and Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Klionsky
Daniel J
DJ
D000588:Amines / Q000378:metabolism; D000818:Animals; D001343:Autophagy; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013194:Staining and Labeling; D015027:Zebrafish / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000378:metabolism
D000588:Amines; D008869:Microtubule-Associated Proteins; D011993:Recombinant Fusion Proteins; C493330:Red DND-99; D029961:Zebrafish Proteins; C542260:map1lc3b protein, zebrafish; D049452:Green Fluorescent Proteins