[Sequential preparation of microvlllous and basal membranes from human placenta].
OBJECTIVE
To improve the technology of isolating paired fractions of the maternal-facing membranes (MVM) and fetal-facing plasma membranes (BM) from a term placenta.


METHODS
The component of buffer was improved based on Illsley method. The time of Mg2+ -aggregated basal membranes was extended. MVM were obtained from the supernatant of low speed centrifugation while BM were further purified on a sucrose step gradient.


RESULTS
Yield for MVM and BM prepared by the method were (0.55 +/- 10.10) mg/g and (0.54 +/- 0.02) mg/g wet weight of placenta. They were enriched 16.87-fold and 11.19-fold as determined by the membrane marker enzymes, alkaline phosphatase (MVM) and adenylate cyclase (BM).


CONCLUSIONS
The modified Illsley method can easily produce both MVM and BM of satisfied quantity from human placenta. It could be applied as a cell molecular model of maternal-fetal exchange interface.
Long
Ning
N
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Xing
Ai-yun
AY
Yang
Xiao-hua
XH
Zhang
Rong
R
Wu
Lin
L
D000469:Alkaline Phosphatase / Q000032:analysis; D001485:Basement Membrane / Q000166:cytology*; D005260:Female; D006801:Humans; D008431:Maternal-Fetal Exchange / Q000502:physiology; D008871:Microvilli / Q000648:ultrastructure*; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D013048:Specimen Handling / Q000379:methods*
D000469:Alkaline Phosphatase