title: Importance of TLR2 in the direct response of T lymphocytes to Schistosoma mansoni antigens.
abstract: The immunomodulatory effect of Schistosoma mansoni antigens has often been attributed to interaction with PRR expressed on APC. Our previous work has shown that S. mansoni-soluble egg antigen (SEA) can induce, together with a Th2 response, TGF-β-dependent Foxp3 expression in naïve CD4(+) T cells from NOD mice. We found that SEA can directly upregulate the expression of surface-bound TGF-β in purified CD4(+) T cells in the absence of accessory cell interactions. In this study, we show that the C-type lectin receptors DEC-205 and galectin-3 were involved in the direct interaction between S. mansoni antigens and CD4(+) T cells. SEA was able to enhance CD4(+) T-cell secretion of bioactive TGF-β in response to TLR2 ligand stimulation, in the absence of APC. We also show that TLR2 expressed on CD4(+) T cells was important for the Foxp3 expression induced by SEA.
authors.affiliation: Department of Pathology, University of Cambridge, Cambridge, UK.
authors.forename: Oliver T
authors.initials: OT
authors.lastname: Burton
authors.forename: Sarah
authors.initials: S
authors.lastname: Gibbs
authors.forename: Nigel
authors.initials: N
authors.lastname: Miller
authors.forename: Frances M
authors.initials: FM
authors.lastname: Jones
authors.forename: Li
authors.initials: L
authors.lastname: Wen
authors.forename: David W
authors.initials: DW
authors.lastname: Dunne
authors.forename: Anne
authors.initials: A
authors.lastname: Cooke
authors.forename: Paola
authors.initials: P
authors.lastname: Zaccone
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D000947:Antigens, Helminth / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D002478:Cells, Cultured; D005260:Female; D051858:Forkhead Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D037502:Galectin 3 / Q000276:immunology / Q000378:metabolism; D015801:Helminth Proteins / Q000276:immunology*; D054884:Host-Pathogen Interactions; D056747:Immunomodulation; D037181:Lectins, C-Type / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism; D012550:Schistosoma mansoni / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000947:Antigens, Helminth; C093569:DEC-205 receptor; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D037502:Galectin 3; D015801:Helminth Proteins; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; D016212:Transforming Growth Factor beta; C079442:gp50 protein, Schistosoma