Effects of the 532-nm and 1,064-nm Q-switched Nd:YAG lasers on collagen turnover of cultured human skin fibroblasts: a comparative study.
Cultured human skin fibroblasts were irradiated twice successively with the 1.5 J/cm(2) of 532-nm and 1,064-nm lasers, respectively. The mRNA of procollagen, matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), heat-shock protein 70 (Hsp70), interleukin-6 (IL-6) and transforming growth factor beta (TGF-beta) were analyzed at 24 and 48 h post-irradiation by using RT-PCR. Both lasers significantly increased the expression of type I and III procollagen, TIMP1, and TIMP2, but decreased MMP1 and MMP2 expression. The 1,064-nm laser initiated TGF-beta expression while the 532-nm laser elicited the increase of Hsp70 and IL-6. The increase/decrease rates of procollagen, TIMPs and MMPs for the 1,064-nm laser were higher than that of the 532-nm laser. Thus, both lasers effectively accelerated collagen synthesis and inhibited collagen degradation. Collagen synthesis induced by the 1,064-nm laser might be partly due to the upregulation of TGF-beta expression, while the increase of Hsp70 and IL-6 might be partly responsible for collagen synthesis stimulated by the 532-nm laser. With the parameters used in this study, the 1,064-nm infrared laser is more effective in promoting the beneficial molecular activities than the 532-nm visible laser.
Dang
Yongyan
Y
School of Life Science, East China Normal University, Shanghai, China.
Ye
Xiyun
X
Weng
Yujing
Y
Tong
Zhi
Z
Ren
Qiushi
Q
D001483:Base Sequence; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D017931:DNA Primers / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics; D053844:Lasers, Solid-State / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D020782:Matrix Metalloproteinases / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000378:metabolism* / Q000528:radiation effects*; D015595:Skin Aging / Q000528:radiation effects; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000235:genetics
D017931:DNA Primers; D018840:HSP70 Heat-Shock Proteins; C508600:IL6 protein, human; D015850:Interleukin-6; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D016212:Transforming Growth Factor beta; D003094:Collagen; D020782:Matrix Metalloproteinases