title: Studies on the toxicity of deoxynivalenol (DON), sodium metabisulfite, DON-sulfonate (DONS) and de-epoxy-DON for porcine peripheral blood mononuclear cells and the Intestinal Porcine Epithelial Cell lines IPEC-1 and IPEC-J2, and on effects of DON and DONS on piglets.
abstract: The in vitro effects of deoxynivalenol (DON), de-epoxy-DON, DON-sulfonate (DONS) and sodium metabisulfite (Na(2)S(2)O(5), SBS) on porcine peripheral blood mononuclear cells (PBMC), and on the Intestinal Porcine Epithelial Cell lines IPEC-1 and IPEC-J2 were examined by using the MTT assay. In addition, an uncontaminated and a DON contaminated triticale were included in diets either untreated (CON, FUS) or SBS treated (CON-SBS, FUS-SBS) and fed to piglets for 28 d starting from weaning. The diet concentrations of DON and DONS amounted to 0.156, 2.312, 0.084 and 0.275 mg and to<0.05, <0.05, <0.05 and 1.841 mg/kg, respectively. PBMC of the so-exposed piglets were also subjected to the MTT assay. Neither DONS and SBS nor de-epoxy-DON affected the viability of PBMC, IPEC-1 and IPEC-J2 significantly up to concentrations of 17, 8 and 23 microM, respectively. For DON, IC(50) values were estimated at 1.2+/-0.1, 1.3+/-0.5 and 3.0+/-0.8 microM for PBMC, IPEC-1 and IPEC-J2, respectively. PBMC from piglets fed the SBS treated diets were characterized by a significantly decreased stimulation index and an increased IgA supernatant concentration with the SBS effect being significantly more pronounced after feeding the FUS-SBS diet. Further studies should clarify the possible impact of SBS on the porcine immune system.
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Braunschweig, Germany. sven.daenicke@fli.bund.de
authors.forename: S
authors.initials: S
authors.lastname: Dänicke
authors.forename: Anne-Kathrin
authors.initials: AK
authors.lastname: Hegewald
authors.forename: S
authors.initials: S
authors.lastname: Kahlert
authors.forename: Jeannette
authors.initials: J
authors.lastname: Kluess
authors.forename: H-J
authors.initials: HJ
authors.lastname: Rothkötter
authors.forename: G
authors.initials: G
authors.lastname: Breves
authors.forename: Susanne
authors.initials: S
authors.lastname: Döll
mesh_terms: D000821:Animal Feed; D000818:Animals; D002460:Cell Line; D004032:Diet; D004063:Digestion; D004305:Dose-Response Relationship, Drug; D020895:Enterocytes / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D008297:Male; D009000:Monocytes / Q000187:drug effects*; D013447:Sulfites / Q000633:toxicity*; D013552:Swine; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D013447:Sulfites; D014255:Trichothecenes; C005200:sodium metabisulfite; C007262:deoxynivalenol