Novel effect of serum on the incorporation of [2-(3)H]mannose into dolichol-bound carbohydrates and proteins.
In the present work we report a serum induced decrease in the rate of [2-(3)H]mannose incorporation into dolichol derivatives and proteins in the embryonic chick optic lobe. This novel effect of serum is widely distributed in sera from different species including man, and it seems to be a unique response of the central nervous system cells. It also appears not to be a generalized toxic effect since it does not affect mannose uptake and deoxyglucose phosphate accumulation. These findings acquire more physiological significance since the in vivo conditions were approached by the preservation of the topological relationship among the cells in each tissue particle and the maintenance of steady state labeling conditions.
Rossi
S G
SG
Instituto de Investigaciones Bioquimicas "Fundacion Campomar" and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Antonio Machado 151, 1405 Buenos Aires, Argentina.
Idoyaga-Vargas
V
V
Carminatti
H
H