[Clinical, histopathological and cytogenetical findings in six cases with primary glioblastoma multiforme].
BACKGROUND
Glioblastoma multiforme (GBM) is the most common malignant primary brain tumor. There is an increasing amount of data demonstrating that the routine histological examination has limited value to predict the tumor biological behaviour.


OBJECTIVE
To compare the clinical, histological and cytogenetical findings in a group of six patients with primary GBM surgically treated in the Department of Neurosurgery at University Hospital "St. Ivan Rilski"- Sofia, Bulgaria.


METHODS
The studied group consisted of three women and three men with average age of 51 years and 6 months. In all patients the diagnosis was histologically confirmed. A microarray comparative genomic hybridization (CGH) analysis of fresh-frozen tumor tissue samples was also made.


RESULTS
In two of the patients the tumor was localized in frontotemporal region, in another two- in frontoparietal, and in the other two- in parietal and occipital respectively. The onset was with headache in three of the cases. The median time between the onset and admission in the clinic was 70 days. Gross-total tumor removal was performed in one patient. In the other five subtotal excision was made. Three of the patients improved after the intervention. One patient deteriorated after the surgery. All patients demonstrated typical histological findings except one who had giant cell subtype of GBM. The microarray CGH analysis determined chromosome 10 monosomy in five patients, trisomy 7 - in four, trisomy 20 - in three, 3q23 deletion - in three, and Yp11.2 deletion - in three.


CONCLUSIONS
GBMs are genetically heterogeneous tumors with different clinical response to standard multimodal treatment regimens. The microarray CGH analysis is a powerful method which can demonstrate the presence of a number of molecular markers with possible predictive value.
Naydenov
E
E
Tzekov
Chr
C
Minkin
K
K
Hadjidekova
S
S
Dimova
I
I
Nachev
S
S
Bussarsky
V
V
Romansky
K
K
Toncheva
D
D
D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization; D020732:Cytogenetic Analysis; D005260:Female; D005784:Gene Amplification; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged; D017384:Sequence Deletion