[Study of signal transduction pathway in the expression of inflammatory factors stimulated by lipopolysaccharides from Porphyromonas endodontalis in osteoblasts].
OBJECTIVE
To quantify the interleukin (IL)-1beta mRNA and IL-6 mRNA expression induced by lipopolysaccharides ([PS) extracted from Porphyromonoas endodontalis (P. endodontalis) in osteoblasts, and to relate P. endodontalis LPS to the bone resorptive pathogenesis in the lesions of chronic apical periodontitis.


METHODS
MG63 cells was pretreated with PD98059 or SB203580 for 1 h and then treated with P. endodontolis LPS for 6 h. The expression of IL-1beta mRNA and IL-6 mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR) technique.


RESULTS
The production of IL-1beta mRNA induced by P. endodontalis LPS decreased in osteoblasts pretreated with PD98059. Both of the production of IL-1beta mRNA and JL-6 mRNA induced by P. endodontalis LPS decreased in osteoblasts pretreated with SB203580.


CONCLUSIONS
The synthesis of IL-1beta mRNA stimulated by Pendodontalis LPS in MG63 probably occur via extracellular signal-regulated kinase (ERK) 1/2 and p38 mitogen activated protein kinase (MAPK) signal transduction system. The synthesis of IL-6 mRNA stimulated by P.endodontalis LPS in MG63 probahly occur via p38MAPK signal transduction system.
Yang
Di
D
Dept. of Endodontics, School of Stomatology, China Medical University, Shenyang 110002, China.
Qiu
Li-hong
LH
Li
Ren
R
Li
Zi-mu
ZM
Li
Chen
C
D006801:Humans; D007093:Imidazoles; D015850:Interleukin-6; D008070:Lipopolysaccharides; D020935:MAP Kinase Signaling System; D010006:Osteoblasts; D045202:Porphyromonas endodontalis; D011725:Pyridines; D012333:RNA, Messenger; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases
D007093:Imidazoles; D015850:Interleukin-6; D008070:Lipopolysaccharides; D011725:Pyridines; D012333:RNA, Messenger; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580