title: Searching for mesophilic Thermotogales bacteria: "mesotogas" in the wild.
abstract: All cultivated Thermotogales are thermophiles or hyperthermophiles. However, optimized 16S rRNA primers successfully amplified Thermotogales sequences from temperate hydrocarbon-impacted sites, mesothermic oil reservoirs, and enrichment cultures incubated at <46 degrees C. We conclude that distinct Thermotogales lineages commonly inhabit low-temperature environments but may be underreported, likely due to "universal" 16S rRNA gene primer bias.
authors.affiliation: Department of Biology, Blindern, 0316 Oslo, Norway. camillaln@gmail.com
authors.forename: Camilla L
authors.initials: CL
authors.lastname: Nesbø
authors.forename: Rajkumari
authors.initials: R
authors.lastname: Kumaraswamy
authors.forename: Marlena
authors.initials: M
authors.lastname: Dlutek
authors.forename: W Ford
authors.initials: WF
authors.lastname: Doolittle
authors.forename: Julia
authors.initials: J
authors.lastname: Foght
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D004785:Environmental Pollutants / Q000032:analysis; D006838:Hydrocarbons / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004785:Environmental Pollutants; D006838:Hydrocarbons; D012336:RNA, Ribosomal, 16S