title: [Simultaneous determination of four active components in Spica Prunellae by HPLC].
abstract: OBJECTIVE
  To develop a HPLC method for simultaneous determination of caffeic acid, rosmarinic acid, oleanolic acid and ursolic acid in Spica Prunellae.
  
  
  METHODS
  After ultrasonic extraction with 75% ethanol solution containing 1% formic acid, the ethanol-extract of Spica Prunellae was analyzed on an Elite SinoChrom ODS-AP column using gradient elution of 0.01% phosphoric acid (A) and acetonitrile (B) at a flow rate of 0.9-1.0 mL x min(-1). A wavelength switch program was used for detection at 330 nm (0-33 min) and 203 nm (33-40 min). The column temperature was set at 20 degrees C and injection volume was 50 microL.
  
  
  RESULTS
  The calibration curves of all analytes were linear. The average recoveries were 93.7%-105.2% with RSDs not more than 4.5%. The contents of caffeic acid, rosmarinic acid, ursolic acid and oleanolic acid in Spica Prunellae were 0.0401-0.0968, 0.99-2.57, 0.243-0.556, 4.06-8.13 mg x g(-1), respectively.
  
  
  CONCLUSIONS
  The described method is sensitive, convenient and accurate, and is suitable for the simultaneous determination of caffeic acid, rosmarinic acid, oleanolic acid and ursolic acid in Spica Prunellae.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. leofang6000@gmail.com
authors.forename: Luo
authors.initials: L
authors.lastname: Fang
authors.forename: Nengming
authors.initials: N
authors.lastname: Lin
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Wu
mesh_terms: D002109:Caffeic Acids / Q000032:analysis; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis; D053630:Depsides / Q000032:analysis; D009828:Oleanolic Acid / Q000032:analysis; D031347:Prunella / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis; D000097123:Rosmarinic Acid; D000097245:Ursolic Acid
chemical_list: D002109:Caffeic Acids; D002934:Cinnamates; D053630:Depsides; D009828:Oleanolic Acid; D014315:Triterpenes; D000097123:Rosmarinic Acid; D000097245:Ursolic Acid; C040048:caffeic acid