title: Structural basis for mechanical force regulation of the adhesin FimH via finger trap-like beta sheet twisting.
abstract: The Escherichia coli fimbrial adhesive protein, FimH, mediates shear-dependent binding to mannosylated surfaces via force-enhanced allosteric catch bonds, but the underlying structural mechanism was previously unknown. Here we present the crystal structure of FimH incorporated into the multiprotein fimbrial tip, where the anchoring (pilin) domain of FimH interacts with the mannose-binding (lectin) domain and causes a twist in the beta sandwich fold of the latter. This loosens the mannose-binding pocket on the opposite end of the lectin domain, resulting in an inactive low-affinity state of the adhesin. The autoinhibition effect of the pilin domain is removed by application of tensile force across the bond, which separates the domains and causes the lectin domain to untwist and clamp tightly around the ligand like a finger-trap toy. Thus, beta sandwich domains, which are common in multidomain proteins exposed to tensile force in vivo, can undergo drastic allosteric changes and be subjected to mechanical regulation.
authors.affiliation: Department of Biological Structure, Box 357420, University of Washington, Seattle, WA 98195, USA.
authors.forename: Isolde
authors.initials: I
authors.lastname: Le Trong
authors.forename: Pavel
authors.initials: P
authors.lastname: Aprikian
authors.forename: Brian A
authors.initials: BA
authors.lastname: Kidd
authors.forename: Manu
authors.initials: M
authors.lastname: Forero-Shelton
authors.forename: Veronika
authors.initials: V
authors.lastname: Tchesnokova
authors.forename: Ponni
authors.initials: P
authors.lastname: Rajagopal
authors.forename: Victoria
authors.initials: V
authors.lastname: Rodriguez
authors.forename: Gianluca
authors.initials: G
authors.lastname: Interlandi
authors.forename: Rachel
authors.initials: R
authors.lastname: Klevit
authors.forename: Viola
authors.initials: V
authors.lastname: Vogel
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Stenkamp
authors.forename: Evgeni V
authors.initials: EV
authors.lastname: Sokurenko
authors.forename: Wendy E
authors.initials: WE
authors.lastname: Thomas
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D000494:Allosteric Regulation; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D018830:Adhesins, Escherichia coli; C058769:fimH protein, E coli; D036781:Fimbriae Proteins