Release of acid-soluble radioactivity from rapidly transported proteins in homogenates of rabbit superior colliculus.
An intraocular injection of [(3)H]proline was used to label rabbit retinal ganglion cells including components subjected to axonal transport. Degradation of rapidly axonally transported labelled proteins was estimated in an in vitro system using homogenates of the nerve terminal regions. The energy requirements of this proteolytic process was characterized, as well as the type of enzymatic system. Evidence was obtained for a proteolytic system, active at a neutral pH, which was dependent upon calcium ions and intact sulfhydryl group.
Gustavsson
S
S
Institute of Neurobiology, University of Göteborg, Göteborg, Sweden.
Karlsson
J O
JO