title: Response surface optimization of the cultivation conditions and medium components for the production of folate by Streptococcus thermophilus.
abstract: Optimization of the cultivation conditions and medium components for folate production by the highest folate producing Streptococcus thermophilus strain RD102 was carried out using a 23 central composite design and surface modelling method of response surface methodology. As folate production was observed to be growth-associated, hence the desired responses selected for the optimization were both folate production and growth. The selected factors based on preliminary investigations were incubation period, concentrations of p-amino benzoic acid (PABA) the growth precursor, and lactose as carbon source. The optimum concentrations of PABA and lactose were found to be 300 micropm and 3% respectively at 72 h incubation. The optimized conditions resulted in an increase in folate production by 26% compared with control conditions (0% PABA and lactose at 37 degrees C). Using the method of experimental factorial design and response surface analysis, it was possible to determine optimal operating conditions to obtain a higher folate production by Strep. thermophilus. Therefore, this study constitutes a step in developing strategies to modulate the folate level to a higher level.
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, India.
authors.forename: Ramya
authors.initials: R
authors.lastname: Iyer
authors.forename: Sudhir Kumar
authors.initials: SK
authors.lastname: Tomar
authors.forename: Ashish Kumar
authors.initials: AK
authors.lastname: Singh
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods; D003470:Culture Media; D005492:Folic Acid / Q000032:analysis / Q000096:biosynthesis*; D005516:Food Microbiology; D008954:Models, Biological; D048249:Streptococcus thermophilus / Q000254:growth & development / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D003470:Culture Media; D005492:Folic Acid