title: Development of "imageable" beads for transcatheter embolotherapy.
abstract: OBJECTIVE
  To develop and characterize radiopaque embolization microspheres capable of in vivo detection with intraprocedural fluoroscopy and computed tomography (CT) imaging and to evaluate their spatial distribution inside target tissues during and after transcatheter embolization.
  
  
  METHODS
  Polyvinyl alcohol hydrogel microspheres were loaded with Lipiodol and examined for iodine content, stability of loading, and conspicuity with fluoroscopy and CT in vitro. Transcatheter embolization of swine liver and kidney was performed with the radiopaque microspheres and spatial distribution was evaluated with intraprocedural fluoroscopy and CT. Ex vivo evaluation was performed with light microscopy and micro-CT.
  
  
  RESULTS
  In vitro analyses demonstrated that radiopaque microspheres could be loaded with sufficient iodine content to be detected with routine fluoroscopy and CT imaging and that such loading was relatively stable. Radiopaque microspheres were visible in vivo with fluoroscopy and CT during transcatheter embolization. CT imaging during embolization procedures demonstrated a dose-dependent relationship in the number and size of visualized embolized arteries. Imaging features of radiopaque microsphere distribution inside target tissues correlated well with ex vivo light microscopic and micro-CT evaluation of microsphere distribution.
  
  
  CONCLUSIONS
  Radiopaque embolization microspheres are visualized during transcatheter embolization with routine intraprocedural fluoroscopy and CT. These radiopaque microspheres provided the three-dimensional spatial distribution of embolic material inside target organs during the procedure, and therefore can provide real-time intraprocedural feedback for the interventional radiologist. These microspheres may be useful for demonstrating the influence of material and technical variability in transcatheter embolization in addition to providing intraprocedural identification of tissue at risk of undertreatment.
authors.affiliation: Department of Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Karun V
authors.initials: KV
authors.lastname: Sharma
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Dreher
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Tang
authors.forename: William
authors.initials: W
authors.lastname: Pritchard
authors.forename: Oscar A
authors.initials: OA
authors.lastname: Chiesa
authors.forename: John
authors.initials: J
authors.lastname: Karanian
authors.forename: Jennifer
authors.initials: J
authors.lastname: Peregoy
authors.forename: Babak
authors.initials: B
authors.lastname: Orandi
authors.forename: David
authors.initials: D
authors.lastname: Woods
authors.forename: Danielle
authors.initials: D
authors.lastname: Donahue
authors.forename: Juan
authors.initials: J
authors.lastname: Esparza
authors.forename: Guy
authors.initials: G
authors.lastname: Jones
authors.forename: Sean L
authors.initials: SL
authors.lastname: Willis
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Lewis
authors.forename: Bradford J
authors.initials: BJ
authors.lastname: Wood
mesh_terms: D000818:Animals; D002404:Catheterization / Q000379:methods*; D003287:Contrast Media; D004621:Embolization, Therapeutic / Q000379:methods*; D007459:Iodized Oil; D008863:Microspheres; D011856:Radiographic Image Enhancement / Q000379:methods*; D015641:Radiography, Interventional / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D003287:Contrast Media; D007459:Iodized Oil