[Protective effect of fibroblast growth factors-21 and rosiglitazone sodium on palmitic acid-induced apoptosis in HIT-T15 cells].
OBJECTIVE
To investigate the protective effect of fibroblast growth factors-21 (FGF-21) and rosiglitazone sodium (RO) on palmitic acid-induced apoptosis in HIT-T15 cells.


METHODS
(1) HIT-T15 cells were treated with 0.25 mmol/L, 0.50 mmol/L and 1.00 mol/L of palmitic acid for 24 hours. (2) FGF-21 [12.50 nmol/L (A), 25.00 nmol/L(B), 50.00 nmol/L(C)], or 1 micromol/L of RO, or a combination of the two were added to the cells treated with 0.50 mmol/L of palmitic acid. Cell apoptosis was measured by flow cytometer. Phosphorylation-c-Jun N-terminal kinases (p-JNK) was detected by immuocytochemistry and Western blot.


RESULTS
(1) The cells treated with palmitic acid has significantly higher apoptosis rates than controls (P < 0.05). (2) FGF-21 reduced apoptosis rates induced by palmitic acid (P < 0.05). But the apoptosis rates remained higher than controls (P < 0.05). A combination of FGF-21 and RO further reduced the apoptosis rates compared with FGF-21 alone (P < 0.05). (3) The cells treated with palmitic acid had higher expression of p-JNK than controls (P < 0.05). FGF-21 and a combination of FGF-21 and RO reduced the expression of p-JNK in cells treated with palmitic acid (P < 0.05).


CONCLUSIONS
(1) Palmitic acid induces apoptosis in HIT-T15 cells in a dose dependent way. (2) The expression of p-JNK induced by palimitic acid is reduced by FGF-21 and the combination of FGF-21 and RO.
Zhang
Lin
L
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Mei
M
Wang
Chun
C
Xiong
Zhong-yun
ZY
An
Zhen-mei
ZM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D006224:Cricetinae; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D050417:Insulin-Secreting Cells / Q000166:cytology*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D019308:Palmitic Acid / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology*
D045162:Thiazolidinediones; C414620:fibroblast growth factor 21; D000077154:Rosiglitazone; D019308:Palmitic Acid; D005346:Fibroblast Growth Factors; D048031:JNK Mitogen-Activated Protein Kinases