title: [Forward genetic screening for zebrafish mutants defective in erythropoiesis].
abstract: OBJECTIVE
  To screen and identify zebrafish mutants with erythropoiesis defects by N-ethyl-N-nitrosourea (ENU) mutagenesis and large-scale forward genetic screening using beta e 1 as the marker.
  
  
  METHODS
  The chemical mutagen ENU was used to treat healthy wild-type male fish (AB strain, F0). The surviving ENU-treated fish were mated with wild-type female fish to generate F1, and further F2 family was generated by F1 family intercross. The adult F2 fish were intercrossed within each F2 family and the resulting F3 embryos from each crossing were subjected to whole mount in situ hybridization (WISH) with the beta e 1 probe. Mutagenesis was performed by treating the male zebrafish with ENU to induce mutations in pre-meiotic germ cells to generate the founders, which were outcrossed to obtained the F1 fish. The F1 fish from different founders were mated to generate the F2 families. F3 embryos from the sibling cross in the F2 family were examined by whole mount in situ hybridization using beta e 1-globin probe. The putative mutants were then characterized with different hematopoiesis markers.
  
  
  CONCLUSIONS
  We identified 4 beta e 1-deficient mutants with erythropoiesis defects, including two with specific erythiod lineage defects and two with concurrent lymphopoiesis defects.
authors.affiliation: Department of Cell Biology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhong-jun
authors.initials: ZJ
authors.lastname: Huo
authors.forename: Zong-hua
authors.initials: ZH
authors.lastname: Wen
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Zhi-bin
authors.initials: ZB
authors.lastname: Huang
authors.forename: Zhao-xia
authors.initials: ZX
authors.lastname: Dai
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Guang
authors.initials: G
authors.lastname: Yan
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Chen
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Pin-yun
authors.initials: PY
authors.lastname: Ma
authors.forename: Wei-hao
authors.initials: WH
authors.lastname: Luo
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Shu
authors.initials: S
authors.lastname: Fan
authors.forename: Jia-jia
authors.initials: JJ
authors.lastname: Zhao
authors.forename: Hong-hui
authors.initials: HH
authors.lastname: Huang
authors.forename: Zi-long
authors.initials: ZL
authors.lastname: Wen
authors.forename: Wen-qing
authors.initials: WQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004920:Erythropoiesis / Q000235:genetics*; D005038:Ethylnitrosourea; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D016254:Mutagenesis, Insertional; D009154:Mutation; D015027:Zebrafish / Q000235:genetics*
chemical_list: D005038:Ethylnitrosourea