Enumeration of Vibrio parahaemolyticus in oyster tissues following artificial contamination and depuration.
OBJECTIVE
To evaluate enumeration of Vibrio parahaemolyticus in oyster tissues following artificial contamination and depuration.


RESULTS
After inoculating with V. parahaemolyticus (ATCC 17802) and incubating for 24 h, the contaminated oysters were depurated with artificial seawater for 14 days. At each step, the tissue homogenate supernatants of oysters were spread-plated onto thiosulfate-citrate-bile salt-sucrose agar, followed by colony confirmation by the polymerase chain reaction. The pathogen was detected in the gills, digestive glands (including stomach, digestive ducts and digestive diverticula), adductor muscle and mantle cilia. After a 48-h depuration period at 17-19 degrees C, the retention rate of V. parahaemolyticus in the gills (28.1%) and digestive glands (13.5%) was higher than that in adductor muscle and mantle cilia (1.4 and 2.4%, respectively).


CONCLUSIONS
The population of V. parahaemolyticus in the digestive glands was the highest among all tissues tested, followed by the gills. The data indicate that digestive glands and gills are good sample candidates for direct monitoring of V. parahaemolyticus contamination in oysters.


CONCLUSIONS
This is the first report on the dynamics of V. parahaemolyticus in various oyster tissues following artificial contamination and depuration. This study provides information to help in monitoring for V. parahaemolyticus in commercial oysters.
Wang
D
D
Department of Food Science & Technology and Bor Luh Food Safety Center, School of Agriculture and Biology, Shanghai JiaoTong University, Shanghai, China.
Yu
S
S
Chen
W
W
Zhang
D
D
Shi
X
X
D000825:Animal Structures / Q000382:microbiology; D000818:Animals; D015169:Colony Count, Microbial / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D008969:Molecular Sequence Data; D010125:Ostreidae / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification*
D003470:Culture Media