title: The pathophysiology of abdominal aortic aneurysm growth: corresponding and discordant inflammatory and proteolytic processes in abdominal aortic and popliteal artery aneurysms.
abstract: OBJECTIVE
  There is remarkable controversy over the processes driving abdominal aneurysm growth. The inherent limitations of animal and human studies hamper elucidation of the key inflammatory and proteolytic processes. Human data are largely derived from surgical specimens that typically reflect the final stages of the disease process and thus do not allow distinction between primary and secondary processes. Clear epidemiologic and genetic associations between abdominal aortic aneurysm (AAA) and popliteal artery aneurysms (PAA) suggest that that these two pathologies share common grounds. On this basis, we reasoned that information of corresponding and discordant processes in these aneurysms might provide critical clues on the processes that are crucial for aneurysm progression.
  
  
  METHODS
  Messenger RNA (semi-quantitative real-time polymerase chain reaction) and protein analysis (enzyme-linked immunosorbent assay, multiplex, Western blotting), and histology were performed on aneurysm wall samples obtained during elective PAA and AAA repair. Nonaneurysmal aorta tissue from organ donors was included as reference.
  
  
  RESULTS
  Messenger RNA and protein analysis showed that PAA and AAA are both characterized by a marked activation of nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) proinflammatory transcription factors, and hyperexpression of interleukin (IL)-6 and IL-8. Discordant findings were found for other inflammatory markers such as interferon-gamma, interferon-inducible protein 10, tumor necrosis factor-alpha, monocyte chemotactic protein-1, and macrophage inflammatory protein 1alpha and beta, which were all lower in PAA. On the cellular level, both pathologies exhibited profuse infiltration of macrophages, neutrophils, and T-helper cells. Results for B cells, plasma cells, and cytotoxic T cells were discordant, with minimal infiltration of these cell types in PAA. Evaluation of protease expression and activation showed that both conditions are dominated by increased matrix metalloproteinase 8 and 9, and cathepsin K, L and S expression and activation.
  
  
  CONCLUSIONS
  This explorative study characterizes degenerative aneurysmal disease general inflammatory conditions that are dominated by profound activation of the NF-kappaB and AP-1 pathways, hyperexpression of IL-6 and IL-8, and neutrophil involvement. Discordant findings for interferon gamma, cytotoxic T cells, B cells, and plasma cells challenge a critical role for these factors in the process of aneurysm growth. Pharmaceutic strategies targeting the common components in AAA and PAA may prove effective for the stabilization of AAA.
authors.affiliation: Department of Vascular Surgery, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Hazem
authors.initials: H
authors.lastname: Abdul-Hussien
authors.forename: Roeland
authors.initials: R
authors.lastname: Hanemaaijer
authors.forename: Robert
authors.initials: R
authors.lastname: Kleemann
authors.forename: Ben F J
authors.initials: BF
authors.lastname: Verhaaren
authors.forename: J Hajo
authors.initials: JH
authors.lastname: van Bockel
authors.forename: Jan H N
authors.initials: JH
authors.lastname: Lindeman
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000783:Aneurysm / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D001012:Aorta, Abdominal / Q000737:chemistry / Q000473:pathology / Q000503:physiopathology*; D017544:Aortic Aneurysm, Abdominal / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D015153:Blotting, Western; D002403:Cathepsins / Q000032:analysis; D017364:Collagenases / Q000032:analysis; D016207:Cytokines / Q000032:analysis; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D008264:Macrophages / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000032:analysis; D020556:Neutrophil Infiltration; D011150:Popliteal Artery / Q000737:chemistry / Q000473:pathology / Q000503:physiopathology*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D006377:T-Lymphocytes, Helper-Inducer / Q000473:pathology; D018808:Transcription Factor AP-1 / Q000032:analysis
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D012333:RNA, Messenger; D018808:Transcription Factor AP-1; D002403:Cathepsins; D017364:Collagenases