title: Phenanthrenes from Dendrobium nobile and their inhibition of the LPS-induced production of nitric oxide in macrophage RAW 264.7 cells.
abstract: Bioactivity-guided isolation of the methanol extract of the stems of Dendrobium nobile yielded a new phenanthrene together with nine known phenanthrenes and three known bibenzyls. Their structures were elucidated by analysis of the spectroscopic data including 2D-NMR. All of the isolates were evaluated for their potential to inhibit the LPS-induced production of nitric oxide in murine macrophage RAW 264.7 cells. Compounds 1-4, 7-13 inhibited nitric oxide production with the IC(50) values ranging from 9.6 microM to 35.7 microM.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Ji Sang
authors.initials: JS
authors.lastname: Hwang
authors.forename: Seon A
authors.initials: SA
authors.lastname: Lee
authors.forename: Seong Su
authors.initials: SS
authors.lastname: Hong
authors.forename: Xiang Hua
authors.initials: XH
authors.lastname: Han
authors.forename: Chul
authors.initials: C
authors.lastname: Lee
authors.forename: Shin Jung
authors.initials: SJ
authors.lastname: Kang
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
mesh_terms: D000818:Animals; D002460:Cell Line; D031667:Dendrobium / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010616:Phenanthrenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts; D013329:Structure-Activity Relationship
chemical_list: D008070:Lipopolysaccharides; D010616:Phenanthrenes; D010936:Plant Extracts; D009569:Nitric Oxide