[MicroRNA profiling in patients with hepatocellular carcinoma].
OBJECTIVE
To investigate the differential expression of microRNAs between human hepatocellular carcinoma (HCC) and liver cirrhosis (LC).


METHODS
The total RNA was extracted from 25 pairs of HCC and LC tissues. microRNA microarray was used to analyze the microRNA expression profiles, and validation of the microarray results was carried out by real-time quantitative RT-PCR (qRT-PCR).


RESULTS
Three microRNAs exhibited higher expression in the HCC samples than in the LC samples. In comparison with the LC samples, the HCC samples showed down-regulated expressions of 9 microRNAs. qRT-PCR verified that has-miR-122a, has-miR-199a, and has-miR-199b were downregulated in HCC, which was in agreement with the microarray results.


CONCLUSIONS
HCC and LC samples have significantly different microRNA expression profiles. These differentially expressed microRNAs may be involved in the pathogensis of HCC.
Wang
Chuan-xi
CX
Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. chuanxiwang@126.com
Song
Wei
W
Li
Zeng-jun
ZJ
Song
Bao
B
Wu
De-hua
DH
Sun
Ai-min
AM
Chen
Long-hua
LH
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008103:Liver Cirrhosis / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged
D035683:MicroRNAs