title: Application of a luminescent bacterial biosensor for the detection of tetracyclines in routine analysis of poultry muscle samples.
abstract: Tetracyclines are extensively used in veterinary medicine. For the detection of tetracycline residues in animal products, a broad array of methods is available. Luminescent bacterial biosensors represent an attractive inexpensive, simple and fast method for screening large numbers of samples. A previously developed cell-biosensor method was subjected to an evaluation study using over 300 routine poultry samples and the results were compared with a microbial inhibition test. The cell-biosensor assay yielded many more suspect samples, 10.2% versus 2% with the inhibition test, which all could be confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Only one sample contained a concentration above the maximum residue limit (MRL) of 100 microg kg(-1), while residue levels in most of the suspect samples were very low (<10 microg kg(-1)). The method appeared to be specific and robust. Using an experimental set-up comprising the analysis of a series of three sample dilutions allowed an appropriate cut-off for confirmatory analysis, limiting the number of samples and requiring further analysis to a minimum.
authors.affiliation: RIKILT-Institute of Food Safety, Wageningen University and Research Centre, NL-6700 AE Wageningen, the Netherlands. mariel.pikkemaat@wur.nl
authors.forename: M G
authors.initials: MG
authors.lastname: Pikkemaat
authors.forename: M L B A
authors.initials: ML
authors.lastname: Rapallini
authors.forename: M T
authors.initials: MT
authors.lastname: Karp
authors.forename: J W A
authors.initials: JW
authors.lastname: Elferink
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000191:economics; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000592:standards; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005062:European Union; D005506:Food Contamination; D005513:Food Inspection / Q000191:economics / Q000379:methods / Q000592:standards; D057230:Limit of Detection; D049453:Luciferases, Bacterial / Q000235:genetics / Q000378:metabolism; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D009876:Operon / Q000187:drug effects / Q000235:genetics; D011200:Poultry; D012097:Repressor Proteins / Q000378:metabolism; D015203:Reproducibility of Results; D013754:Tetracyclines / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors; D019155:Veterinary Drugs / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D012097:Repressor Proteins; D013754:Tetracyclines; D019155:Veterinary Drugs; D049453:Luciferases, Bacterial