[Effect of Oviductus Ranae on Cyclin D1, CDK6 and P15 expressions in the liver tissue of aged male rats].
OBJECTIVE
To investigate the effect of Oviductus Ranae (OR) on the expressions of CyclinD1, CDK6 and P15 in the liver of aged male rats.


METHODS
Eighteen male SD rats were randomly divided into 3 equal groups, namely the OR group, VE group and ageing model group. The rats received subcutaneous injection of D-galactose for 6 weeks to establish the aging models, and another 6 rats were injected daily with normal saline (NS) to serve as the normal control group. From the third week of the experiment, the rats were given oral OR or Vitamin E (VE) accordingly till the sixth week. After completion of the drug administration, all the rats were sacrificed for detecting the expressions of CyclinD1, CDK6 and P15 in the liver tissue by Western blotting.


RESULTS
The relative expression levels of CyclinD1, CDK6 and P15 in the liver of the rats in the OR group were 41.73-/+0.54, 23.29-/+0.30 and 1.49-/+0.30, respectively, significantly up-regulated as compared with those in the ageing model group (P<0.01). The expressions of the proteins were obviously down-regulated in the model group in comparison with those in the normal control group.


CONCLUSIONS
OR treatment can lower the expressions of Cyclin D1 and CDK6 in the liver to enhance the liver cell proliferation in aged male rats. OR also promotes the expression of P15 through a feedback mechanism to prevent excessive proliferation of the cells. The effect of OR against ageing is mediated possibly by up-regulation of the proteins associated with the cell proliferation in the liver, a mechanism different from that of VE.
Yao
Hui
H
College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. fsyaohui@126.com
Wang
Xiao-juan
XJ
Huang
Li-ping
LP
Diao
Jian-xin
JX
Deng
Hong-zhu
HZ
D000375:Aging / Q000378:metabolism*; D000818:Animals; D019938:Cyclin D1 / Q000378:metabolism*; D051361:Cyclin-Dependent Kinase 6 / Q000378:metabolism*; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008419:Materia Medica / Q000494:pharmacology*; D051381:Rats
C530106:Ccnd1 protein, rat; D050762:Cyclin-Dependent Kinase Inhibitor p15; D008419:Materia Medica; C539466:Oviductus Ranae; D019938:Cyclin D1; C495913:Cdk6 protein, rat; D051361:Cyclin-Dependent Kinase 6