title: Handheld mechanical cell lysis chip with ultra-sharp silicon nano-blade arrays for rapid intracellular protein extraction.
abstract: This paper presents a handheld mechanical cell lysis chip with ultra-sharp nano-blade arrays fabricated by simple and cost effective crystalline wet etching of (110) silicon. The ultra-sharp nano-blade array is simply formed by the undercutting of (110) silicon during the crystalline wet etching process. Cells can be easily disrupted by the silicon nano-blade array without the help of additional reagents or electrical sources. Based on the bench-top test of the proposed device, a handheld mechanical cell lysis chip with the nano-blade arrays is designed and fabricated for direct connection to a commercial syringe. The direct connection to a syringe provides rapid cell lysis, easy handling, and minimization of the lysate dead volume. The protein concentration in the cell lysate obtained by the proposed lysis chip is quantitatively comparable to the one prepared by a conventional chemical lysis method.
authors.affiliation: Department of Nanobio Materials and Electronics, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju, Republic of Korea 500-712.
authors.forename: Sung-Sik
authors.initials: SS
authors.lastname: Yun
authors.forename: Sang Youl
authors.initials: SY
authors.lastname: Yoon
authors.forename: Min-Kyung
authors.initials: MK
authors.lastname: Song
authors.forename: Sin-Hyeog
authors.initials: SH
authors.lastname: Im
authors.forename: Sohee
authors.initials: S
authors.lastname: Kim
authors.forename: Jong-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Sung
authors.initials: S
authors.lastname: Yang
mesh_terms: D002458:Cell Fractionation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007424:Intracellular Fluid / Q000737:chemistry; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D011506:Proteins