Contribution of NOD2 to lung inflammation during Staphylococcus aureus-induced pneumonia.
Staphylococcus aureus is the most commonly found Gram-positive bacterium in patients admitted in intensive-care units, causing septicaemia or pneumonia. In this work, we investigated the role of NOD2 in S. aureus-induced pneumonia. We found that the absence of NOD2 affected weight loss and recovery speed. Nod2-/- mice showed a reduced lung inflammation in comparison to wild-type animals, with lower presence of cytokines in broncho-alveolar lavage fluids and reduced recruitment of neutrophils. Furthermore, histological analysis of the lungs revealed less severe lesions in Nod2-/- mice at day 2 and day 7 post-infection. In conclusion, we demonstrated that NOD2 is not a crucial receptor to fight S. aureus-induced pneumonia, but that it contributes to the inflammatory response in the lungs. Interestingly, the absence of NOD2 led to a lesser inflammation and was finally beneficial for the animal recovery.
Kapetanovic
Ronan
R
Institut Pasteur, Unité Cytokines & Inflammation, Département Infection et épidémiologie, 28 rue du Dr Roux, F-75015 Paris, France.
Jouvion
Gregory
G
Fitting
Catherine
C
Parlato
Marianna
M
Blanchet
Charlène
C
Huerre
Michel
M
Cavaillon
Jean-Marc
JM
Adib-Conquy
Minou
M
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008853:Microscopy; D009504:Neutrophils / Q000276:immunology; D053473:Nod2 Signaling Adaptor Protein / Q000172:deficiency / Q000276:immunology*; D011014:Pneumonia / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D011023:Pneumonia, Staphylococcal / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D013211:Staphylococcus aureus / Q000472:pathogenicity*
D016207:Cytokines; D053473:Nod2 Signaling Adaptor Protein; C495945:Nod2 protein, mouse