Expression of the T-cell-specific adapter protein in oral epithelium.
The multifunctional T-cell-specific adapter protein (TSAd) was originally described in T cells but is also expressed in epithelial cells from the respiratory tract and in endothelium. In this study, we found expression of TSAd messenger RNA (mRNA) and protein in both human and murine oral mucosal epithelium as well as in human primary oral keratinocyte cell cultures. In TSAd(-/-) mice, the mucosa and skin appeared macroscopically normal, but severe disturbances were observed in the fine structures of the basal membrane and intercellular epithelial spaces upon analysis using transmission electron microscopy. Oral epithelial cells from TSAd(-/-) mice displayed decreased migration compared with cells from wild-type mice, whereas overexpression of TSAd in a human epithelial cell line resulted in impaired proliferation. This study is the first to show that TSAd is expressed in normal oral mucosa, that it is important for the normal ultrastructural morphology of the epithelium and the basal membrane, and that it is involved in the migration and proliferation of oral keratinocytes.
Kolltveit
Kristin M
KM
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Norway. k.m.kolltveit@odont.uio.no
Schreurs
Olav
O
Ostrem
Janet
J
Søland
Tine M
TM
Khuu
Cuong
C
Berge
Tone
T
Messelt
Edward
E
Hayashi
Katsuhiko
K
Granum
Stine
S
Spurkland
Anne
A
Schenck
Karl
K
D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis* / Q000235:genetics; D000818:Animals; D001485:Basement Membrane / Q000166:cytology / Q000648:ultrastructure; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000166:cytology / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000166:cytology / Q000648:ultrastructure; D007633:Keratins / Q000032:analysis; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D046529:Microscopy, Electron, Transmission; D009061:Mouth Mucosa / Q000166:cytology* / Q000648:ultrastructure; D014162:Transfection
D048868:Adaptor Proteins, Signal Transducing; D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D008562:Membrane Glycoproteins; C402726:Sh2d2a protein, mouse; C029967:nidogen; D007633:Keratins