The roles of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells.
OBJECTIVE
To explore the function of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells, and discuss the signal transduetion mechanism of HepG2 cells in the apoptosis process induced by DADS by using the inhibitors of p38MAPK (SB203580) and caspase-3 (Z-DEVD-FMK).


METHODS
After the human HepG2 cells had been treated with the DADS and inhibitors for 24 h, cell viability was determined by the MTT method, apoptosis was evaluated by flow cytometry (FCM) and the expressions of p38MAPK and caspase-3 were measured by western-blot.


RESULTS
Our results indicated that DADS activities the p38MAPK and caspase-3, but the inhibitors, SB203580 and Z-DEVD-FMK (for p38MAPKand for caspase-3, respectively), both have the effect of inhibitory activity on P38MAPK and caspase-3. Furthermore, a combination treatment with both DADS and inhibitor (SB203580 or Z-DEVD-FMK) decreases the inhibitory and apoptotic activity of HepG2 cells increased compared with DADS-treated.


CONCLUSIONS
Our data indicate that p38MAPK and caspase-3 are involved in the process of DADS-induced apoptosis in human HepG2 cells and interact with each other.
Ji
Chunxiao
C
College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, China.
Ren
Fenglian
F
Ma
Heng
H
Xu
Ming
M
D000498:Allyl Compounds / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism*; D004220:Disulfides / Q000494:pharmacology*; D005434:Flow Cytometry; D005737:Garlic / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D000498:Allyl Compounds; D000970:Antineoplastic Agents; D004220:Disulfides; C028009:diallyl disulfide; D048051:p38 Mitogen-Activated Protein Kinases; D053148:Caspase 3