title: Evidence for a premature termination mechanism of subgenomic mRNA transcription in a carmovirus.
abstract: The transcriptional mechanism utilized by turnip crinkle carmovirus to synthesize subgenomic (sg) mRNAs was investigated by analyzing viral RNAs and their associated regulatory RNA elements. In vivo analyses revealed the following: (i) that minus-strand sg RNAs are detectable in infections, (ii) that minus-strand sg RNA accumulation can be partially uncoupled from that of their plus-strand sg mRNA counterparts, and (iii) that an RNA secondary structure located upstream of the sg mRNA start site mediates transcription by functioning in the plus strand of the viral genome. Collectively, these observations are consistent with this carmovirus using a premature termination mechanism for sg mRNA transcription.
authors.affiliation: Department of Biology, York University, Toronto, Ontario, Canada.
authors.forename: Baodong
authors.initials: B
authors.lastname: Wu
authors.forename: Stefanie
authors.initials: S
authors.lastname: Oliveri
authors.forename: Jana
authors.initials: J
authors.lastname: Mandic
authors.forename: K Andrew
authors.initials: KA
authors.lastname: White
mesh_terms: D001483:Base Sequence; D029688:Brassica napus / Q000821:virology*; D019184:Carmovirus / Q000502:physiology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral