Short report: Tissue-specific expression profiles of the uncoupling protein family in normal control mice and genetically ob/ob mice.
Uncoupling proteins (UCPs) located in the inner mitochondrial membrane are involved in the regulation of energy balance. Thus far, 5 UCP isoforms have been identified, but controversies exist in the research focused on the function of the UCPs (except UCP1) in the pathogenesis of obesity. Because of the known cross-reactivity of the antibodies presently available for the detection of UCP proteins, this study systematically analyzed the differential tissue expression profiles of the 5 UCP isoforms in lean control mice and ob/ob mice by using real-time polymerase chain reaction (PCR) analysis. The results show that the tissue-specific expression patterns of individual isoforms in normal and ob/ob mice are considerably different; this will provide new insights into the functions of UCPs in the pathogenesis of genetic obesity.
Zhang
Chun-Mei
CM
Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
Zhang
Min
M
Zhu
Jin-Gai
JG
Ji
Chen-Bo
CB
Zhu
Chun
C
Kou
Chun-Zhao
CZ
Qin
Da-Ni
DN
Tong
Mei-Ling
ML
Guo
Xi-Rong
XR
D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D007473:Ion Channels / Q000235:genetics*; D051379:Mice; D008820:Mice, Obese; D024101:Mitochondrial Proteins / Q000235:genetics*; D009765:Obesity / Q000209:etiology / Q000235:genetics*; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D000071256:Uncoupling Protein 1
D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1