Differential expression of the miR-200 family microRNAs in epithelial and B cells and regulation of Epstein-Barr virus reactivation by the miR-200 family member miR-429.
The miR-200 microRNA family is important for maintaining the epithelial phenotype, partially through suppressing ZEB1 and ZEB2. Since ZEB1 inhibits Epstein-Barr virus (EBV) reactivation, we hypothesized that expression of miR-200 family members in epithelial cells may partly account for higher levels of EBV reactivation in this tissue (relative to nonplasma B cells). Here we show that, whereas miR-200 family members are expressed in epithelial cells, their expression is low in latently infected B cells. Furthermore, the miR-200 family member miR-429 shows elevated expression in plasma cell lines and is induced by B-cell-receptor activation in Akata cells. Lastly, expression of miR-429 can break latency.
Lin
Zhen
Z
Department of Pathology, SL-79, Tulane Health Sciences Center, New Orleans, Louisiana 70112, USA.
Wang
Xia
X
Fewell
Claire
C
Cameron
Jennifer
J
Yin
Qinyan
Q
Flemington
Erik K
EK
D001402:B-Lymphocytes / Q000821:virology*; D004847:Epithelial Cells / Q000821:virology*; D020869:Gene Expression Profiling; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D014775:Virus Activation
C533896:MIRN200 microRNA, human; C546363:MIRN429 microRNA, human; D035683:MicroRNAs