Influence of polygenetic polymorphisms on the susceptibility to non-alcoholic fatty liver disease of Chinese people.
OBJECTIVE
The aim of this study was to investigate the influence of polygenetic polymorphisms, which play a role in the pathogenesis of metabolic syndrome, on the susceptibility to non-alcoholic fatty liver disease (NAFLD) of Chinese people.


METHODS
The subjects were selected from an epidemiological survey in the Guangdong province of southern China. In each polymorphism study, 50-117 subjects who met the diagnostic criteria of NAFLD and had typical clinical and ultrasonographic findings were placed into the case group. Using a nested case-control design, the same numbers of matched people without NAFLD were included as controls. Single nucleotide polymorphisms (SNP) at nine positions in seven candidate genes were tested. These SNP were found to be associated with the pathogenesis of metabolic syndrome. Genetic analyses were performed using genomic DNA extracted from peripheral blood leukocytes. Polymerase chain reaction-restriction fragment length polymorphism was applied to detect SNP.


RESULTS
Most candidate genes' SNP were associated with susceptibility to NAFLD. Some showed positive relationships (increased risk): tumor necrosis factor-alpha-238, adiponectin-45, leptin-2548, peroxisome proliferator-activated receptors-161 and phosphatidyletha-nolamine N-methyltransferase-175. Other SNP demonstrated a negative association (decreased risk): adiponectin-276 and hepatic lipase-514. Only two were not associated: tumor necrosis factor-alpha-380 and peroxisome proliferator-activated receptors-gamma co-activator-1alpha-482.


CONCLUSIONS
Most candidate genes' SNP examined in metabolic syndrome patients were associated with susceptibility to NAFLD.
Zhou
Yong-Jian
YJ
Department of Gastroenterology and Hepatology, First Municipal People's Hospital of Guangzhou, Guangzhou Medical College, Guangzhou, China.
Li
Yu-Yuan
YY
Nie
Yu-Qiang
YQ
Yang
Hui
H
Zhan
Qi
Q
Huang
Jian
J
Shi
Sheng-Li
SL
Lai
Xiao-Bo
XB
Huang
Hong-Li
HL
D052242:Adiponectin / Q000235:genetics; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D005234:Fatty Liver / Q000000981:diagnostic imaging / Q000208:ethnology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D020738:Leptin / Q000235:genetics; D008049:Lipase / Q000235:genetics; D008099:Liver / Q000000981:diagnostic imaging; D008297:Male; D024821:Metabolic Syndrome / Q000000981:diagnostic imaging / Q000208:ethnology / Q000235:genetics*; D008875:Middle Aged; D047495:PPAR gamma / Q000235:genetics; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010641:Phenotype; D050918:Phosphatidylethanolamine N-Methyltransferase / Q000235:genetics; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D018570:Risk Assessment; D012307:Risk Factors; D014157:Transcription Factors / Q000235:genetics; D014463:Ultrasonography
C499918:ADIPOQ protein, human; D052242:Adiponectin; D005819:Genetic Markers; D006360:Heat-Shock Proteins; C504173:LIPC protein, human; D020738:Leptin; D047495:PPAR gamma; C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D014157:Transcription Factors; C494489:PEMT protein, human; D050918:Phosphatidylethanolamine N-Methyltransferase; D008049:Lipase