title: Laser desorption-ionization of lipid transfers: tissue mass spectrometry imaging without MALDI matrix.
abstract: Mass spectrometry imaging of tissue-lipid transfers without MALDI matrix is demonstrated. Commercially available nanostructured surfaces (nano-assisted laser desorption-ionization or NALDI) are used as substrates for imprinting of tissue sections. The lithographic transfers are then washed and the two-dimensional distribution of the lipids is imaged by laser desorption-ionization mass spectrometry. The NALDI imaging of lipid transfers is compared with standard MALDI imaging of matrix-coated tissue sections. The obtained images are of the same quality, and no spatial information is lost due to the imprinting process. NALDI imaging is faster due to the absence of the time-consuming matrix deposition step, and the NALDI mass spectra are less complex and easier to interpret than standard MALDI. In this particular application example, NALDI mass spectrometry is able to identify the same lipid species as MALDI mass spectrometry and provides better distinction between kidney and adrenal gland tissues based on the lipid analysis.
authors.affiliation: Laboratory of Molecular Structure Characterization, Institute of Microbiology of the ASCR, v.v.i., Videnska 1083, Prague 4, CZ-142 20, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Vidová
authors.forename: Petr
authors.initials: P
authors.lastname: Novák
authors.forename: Martin
authors.initials: M
authors.lastname: Strohalm
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Pól
authors.forename: Vladimír
authors.initials: V
authors.lastname: Havlícek
authors.forename: Michael
authors.initials: M
authors.lastname: Volný
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods*; D007668:Kidney / Q000737:chemistry; D007834:Lasers; D008055:Lipids / Q000032:analysis*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods
chemical_list: D008055:Lipids