Identification of an intergenic region that is not essential for replication of goatpox virus.
A recombinant goatpox virus was constructed in which an enhanced green fluorescent protein gene was inserted under the control of the 11K late promoter, a guanine phosphoribosyltransferase gene was inserted under the control of the 7.5K early/late promoter, and exogenous genes were inserted into an intergenic region between loci gp_24 and gp_24.5 of the recombinant genome. Analysis of protein expression showed that LT cells infected with only the recombinant virus produced specific fluorescence. A comparative growth assay demonstrated the stability of the recombinant virus at the replication level. These results suggest that the intergenic region is not essential for replication of goatpox virus.
Ma
Wenge
W
Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, People's Republic of China. mawenge2004@yahoo.com.cn
Wang
Fang
F
Yu
Li
L
Xue
Ying
Y
Wang
Yan
Y
Wei
Jie
J
Huang
Jiong
J
Fu
Zihua
Z
D000818:Animals; D018151:Capripoxvirus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D002460:Cell Line; D021901:DNA, Intergenic / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D013737:Testis / Q000166:cytology / Q000821:virology; D014162:Transfection; D014779:Virus Replication
D021901:DNA, Intergenic; D008164:Luminescent Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D007041:Hypoxanthine Phosphoribosyltransferase