title: A microplate-based method for real-time monitoring of Saccharomyces cerevisiae viability.
abstract: Measuring the viability of the cells after chemical or physical stresses is a routine method in the determination of phenotypes in yeast. Numerous analytical techniques have been applied to the measurement of yeast cell viability, but few of them are suitable for real-time monitoring. Here we propose a microplate-based method for real-time monitoring of Saccharomyces cerevisiae cell viability. This method is also suitable for high-throughput measurement.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, People's Republic of China.
authors.forename: Yan-Rui
authors.initials: YR
authors.lastname: Ye
authors.forename: Mei-Li
authors.initials: ML
authors.lastname: Ye
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D057166:High-Throughput Screening Assays; D008751:Methylene Blue / Q000737:chemistry; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D013312:Stress, Physiological
chemical_list: D008751:Methylene Blue