Purification, chemical modification and immunostimulating activity of polysaccharides from Tremella aurantialba fruit bodies.
Ultrafiltration and a series of chromatographic steps were used to isolate and purify polysaccharides from Tremella aurantialba fruit bodies. Three crude fractions (TAP50w, TAP10-50w, and TAP1-10w), five semi-purified fractions (TAPA-TAPE), and one purified fraction (TAPA1) were obtained. A sulfated derivative of TAPA1 (TAPA1-s) was prepared by chemical modification. The immunostimulating activity of the polysaccharide fractions in vitro was determined using the mouse spleen lymphocyte proliferation assay. Of the three crude fractions tested, cell proliferation rates were increased most by TAP50w. Furthermore, TAPA1-s was markedly more stimulatory than TAPA1, indicating that sulfonation was an effective way to enhance the immunostimulating activity of polysaccharide.
Du
Xiu-ju
XJ
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Zhang
Jing-song
JS
Yang
Yan
Y
Tang
Qing-jiu
QJ
Jia
Wei
W
Pan
Ying-jie
YJ
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D001487:Basidiomycota / Q000378:metabolism*; D002478:Cells, Cultured; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification*
D000276:Adjuvants, Immunologic; D011134:Polysaccharides