SiRNA-loaded multi-shell nanoparticles incorporated into a multilayered film as a reservoir for gene silencing.
In this study, we presented a new type of coating based on polyelectrolyte multilayers containing sequentially adsorbed active shRNA calcium phosphate nanoparticles for locally defined and temporarily variable gene silencing. Therefore, we investigated multi-shell calcium phosphate-shRNA nanoparticles embedded into a polyelectrolyte multilayer for gene silencing. As model system, we synthesized triple-shell calcium phosphate-shRNA nanoparticles (NP) and prepared polyelectrolyte multilayers films made of nanoparticles and poly-(L-lysine) (PLL). The biological activities of these polyelectrolyte multilayers films were tested by the production of osteopontin and osteocalcin in the human osteoblasts (HOb) which were cultivated on the PEM films. This new strategy can be used to efficiently control the bone formation and could be applicable in tissue engineering.
Zhang
Xin
X
Institut National de la Santé et de la Recherche Médicale, INSERM, Unité 977, Faculté de Médecine, Strasbourg, France.
Kovtun
Anna
A
Mendoza-Palomares
Carlos
C
Oulad-Abdelghani
Mustapha
M
Fioretti
Florence
F
Rinckenbach
Simon
S
Mainard
Didier
D
Epple
Matthias
M
Benkirane-Jessel
Nadia
N
D002478:Cells, Cultured; D020868:Gene Silencing; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D010006:Osteoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D053495:Osteopontin / Q000096:biosynthesis; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction
D034741:RNA, Small Interfering; D015675:Osteocalcin; D053495:Osteopontin