title: Population-based analysis of Actinobacillus pleuropneumoniae ApxIVA for use as a DIVA antigen.
abstract: APXIVA is an RTX toxin of Actinobacillus pleuropneumoniae that is a candidate antigen to differentiate infected from vaccinated animals (DIVA). Insertion of ISApl1 into the apxIVA gene is known to compromise an APXIVA-based DIVA approach, as is potentially a TGG to TGA mutation in the apxIVA gene. ISApl1 was found in 63/349 (18.1%) A. pleuropneumoniae isolates from England and Wales including serovars 2, 3, 6-8 and 12. No ISApl1 insertions into apxIVA were found. Only two serovar 3 isolates contained the TGG to TGA mutation. We conclude that an ApxIVA-based DIVA approach would potentially be viable in England and Wales.
authors.affiliation: Molecular Infectious Diseases Group, Department of Paediatrics, Imperial College London, St Mary's Campus, London, W2 1PG, UK.
authors.forename: Ciaragh
authors.initials: C
authors.lastname: O'Neilla
authors.affiliation: Molecular Infectious Diseases Group, Department of Paediatrics, Imperial College London, St Mary's Campus, London, W2 1PG, UK.
authors.forename: Sophie C P
authors.initials: SCP
authors.lastname: Jones
authors.affiliation: Molecular Infectious Diseases Group, Department of Paediatrics, Imperial College London, St Mary's Campus, London, W2 1PG, UK.
authors.forename: Janine T
authors.initials: JT
authors.lastname: Bossé
authors.affiliation: AFBI-VSD, Stoney Road, Stormont, Belfast, BT4 3SD, UK.
authors.forename: Conrad M
authors.initials: CM
authors.lastname: Watson
authors.affiliation: Veterinary Laboratories Agency (VLA) Bury St Edmunds, Rougham Hill, Bury St Edmunds, Suffolk, IP33 2RX, UK.
authors.forename: Susanna M
authors.initials: SM
authors.lastname: Williamson
authors.affiliation: Department of Pathology and Infectious Diseases, Royal Veterinary College, Hawkshead Lane, North Mimms, Herts AL9 7TA, UK.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Rycroft
authors.affiliation: Molecular Infectious Diseases Group, Department of Paediatrics, Imperial College London, St Mary's Campus, London, W2 1PG, UK.
authors.forename: J
authors.initials: J
authors.lastname: Simon Kroll
authors.affiliation: AFBI-VSD, Stoney Road, Stormont, Belfast, BT4 3SD, UK.
authors.forename: Helen M
authors.initials: HM
authors.lastname: Hartley
authors.affiliation: Molecular Infectious Diseases Group, Department of Paediatrics, Imperial College London, St Mary's Campus, London, W2 1PG, UK.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Langford
mesh_terms: D000189:Actinobacillus Infections / Q000276:immunology / Q000517:prevention & control; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D004269:DNA, Bacterial / Q000235:genetics; D004739:England; D016254:Mutagenesis, Insertional; D009154:Mutation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014852:Wales
chemical_list: D000942:Antigens, Bacterial; C122177:ApxIVA protein, Actinobacillus pleuropneumoniae; D001426:Bacterial Proteins; D004269:DNA, Bacterial