title: A lanthanide-complex-based ratiometric luminescent probe specific for peroxynitrite.
abstract: A lanthanide-complex-based ratiometric luminescence probe specific for peroxynitrite (ONOO(-)), 4'-(2,4-dimethoxyphenyl)-2,2':6',2''-terpyridine-6,6''-diyl]bis(methylenenitrilo)tetrakis(acetate)-Eu(3+)/Tb(3+) ([Eu(3+)/Tb(3+)(DTTA)]), has been designed and synthesized. Both [Eu(3+)(DTTA)] and [Tb(3+)(DTTA)] are highly water soluble with large stability constants at approximately 10(20), and strongly luminescent with luminescence quantum yields of 10.0 and 9.9%, respectively, and long luminescence lifetimes of 1.38 and 0.26 ms, respectively. It was found that the luminescence of [Tb(3+)(DTTA)] could be quenched by ONOO(-) rapidly and specifically in aqueous buffers, while that of [Eu(3+)(DTTA)] did not respond to the addition of ONOO(-). Thus, by simply mixing [Eu(3+)(DTTA)] and [Tb(3+)(DTTA)] in an aqueous buffer, a ratiometric luminescence probe specific for time-gated luminescence detection of ONOO(-) was obtained. The performance of [Tb(3+)(DTTA)] and [Eu(3+)/Tb(3+)(DTTA)] as the probes for luminescence imaging detection of ONOO(-) in living cells was investigated. The results demonstrated the efficacy and advantages of the new ratiometric luminescence probe for highly sensitive luminescence bioimaging application.
authors.affiliation: Department of Instrumentation and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Cuihong
authors.initials: C
authors.lastname: Song
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Ye
authors.forename: Guilan
authors.initials: G
authors.lastname: Wang
authors.forename: Jingli
authors.initials: J
authors.lastname: Yuan
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Guan
mesh_terms: D005063:Europium / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D028581:Lanthanoid Series Elements / Q000138:chemical synthesis* / Q000737:chemistry*; D008024:Ligands; D049449:Luminescence; D049408:Luminescent Agents / Q000138:chemical synthesis* / Q000737:chemistry; D008163:Luminescent Measurements; D015335:Molecular Probes / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D030421:Peroxynitrous Acid / Q000737:chemistry*; D012995:Solubility; D013725:Terbium / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D028581:Lanthanoid Series Elements; D008024:Ligands; D049408:Luminescent Agents; D015335:Molecular Probes; D009942:Organometallic Compounds; D014867:Water; D013725:Terbium; D030421:Peroxynitrous Acid; D005063:Europium