Trifluralin herbicide-induced resistance of melon to fusarium wilt involves expression of stress- and defence-related genes.
SUMMARY To identify genes involved in trifluralin herbicide-induced resistance of melon to Fusarium oxysporum f. sp. melonis, suppression subtractive hybridization (SSH) and cDNA-amplified fragment-length polymorphism (cDNA-AFLP) were used. A total of 123 clones-60 of which have never been isolated from melon-were isolated, sequenced and annotated. A significant proportion (35%) of the total 123 clones exhibited similarity to genes that have been formerly described as stress- or defence-related. Thirty-two selected clones were subjected to a detailed expression analysis, one-third of which were found to be up-regulated in response to trifluralin treatment and/or fusarium inoculation. The putative roles of seven of these clones in stress are discussed. Furthermore, the expression of four stress-related and up-regulated genes was enhanced when the plants were subjected to salinity stress, suggesting that trifluralin induces a general stress response which protects the plant against fusarium wilt.
Lotan-Pompan
Maya
M
Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat-Yishay 30095, Israel.
Cohen
Ron
R
Yarden
Oded
O
Portnoy
Vitaly
V
Burger
Yosef
Y
Katzir
Nurit
N