The effect of amino acid infusion on anesthesia-induced hypothermia in muscle atrophy model rats.
An infusion of amino acids stimulates heat production in skeletal muscle and then attenuates the anesthesia-induced hypothermia. However, in a clinical setting, some patients have atrophic skeletal muscle caused by various factors. The present study was therefore conducted to investigate the effect of amino acids on the anesthesia-induced hypothermia in the state of muscle atrophy. As the muscle atrophy model, Sprague-Dawley rats were subjected to hindlimb immobilization for 2 wk. Normal rats and atrophy model rats were randomly assigned to one of the two treatment groups: saline or amino acids (n=8 for each group). Test solutions were administered intravenously to the rats under sevoflurane anesthesia for 180 min, and the rectal temperature was measured. Plasma samples were collected for measurement of insulin, blood glucose, and free amino acids. The rectal temperature was significantly higher in the normal-amino acid group than in the muscle atrophy-amino acid group from 75 to 180 min. The plasma insulin level was significantly higher in the rats given amino acids than in the rats given saline in both normal and model groups. In the rats given amino acids, plasma total free amino acid concentration was higher in the model group than in the normal group. These results indicate that skeletal muscle plays an important role in changes in body temperature during anesthesia and the effect of amino acids on anesthesia-induced hypothermia decreases in the muscle atrophy state. In addition, intravenous amino acids administration during anesthesia induces an increase in the plasma insulin level.
Kanazawa
Masahiro
M
Department of Anesthesiology, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan. kanazawa@is.icc.u-tokai.ac.jp
Ando
Satoko
S
Tsuda
Michio
M
Suzuki
Toshiyasu
T
D000596:Amino Acids / Q000097:blood / Q000494:pharmacology*; D000704:Analysis of Variance; D000768:Anesthesia, General / Q000009:adverse effects*; D018685:Anesthetics, Inhalation / Q000008:administration & dosage / Q000009:adverse effects / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D001831:Body Temperature / Q000187:drug effects; D004195:Disease Models, Animal; D007035:Hypothermia / Q000097:blood / Q000139:chemically induced* / Q000517:prevention & control*; D007328:Insulin / Q000097:blood; D008297:Male; D008738:Methyl Ethers / Q000008:administration & dosage / Q000009:adverse effects / Q000097:blood; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D009133:Muscular Atrophy / Q000150:complications / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane; D012965:Sodium Chloride / Q000008:administration & dosage
D000596:Amino Acids; D018685:Anesthetics, Inhalation; D001786:Blood Glucose; D007328:Insulin; D008738:Methyl Ethers; D000077149:Sevoflurane; D012965:Sodium Chloride