title: DOX-loaded PEG-PLGA and Pluronic copolymer composite micelles enhances cytotoxicity and the intracellular accumulation of drug in DOX-resistant tumor cells.
abstract: In the present study, doxorubicin (DOX) loaded polyethyleneglycol-poly (DL-lactic-co-glycolic acid) micelle as well as composite micelles composed polyethyleneglycol- poly(DL-lactic-co-glycolic acid) (PEG-PLGA) and Pluronic 105 (P105) were constructed. The micelles, with diameter around 106 nm and 85 nm respectively, were prepared by solvent evaporation method. The results showed that the encapsulation of DOX in micelles could significantly enhance its cytotoxicity in a DOX resistant tumor cell line, K562/DOX. The combination of PEG-PLGA and Pluronic further improved both the tumor-suppressive activity and the intracellular accumulation of DOX, indicating that the composite micelles would be potential to reverse the multidrug resistance in tumor cells.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, P.R. China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Diao
authors.forename: Min
authors.initials: M
authors.lastname: Han
authors.forename: Ping-Tian
authors.initials: PT
authors.lastname: Ding
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Chen
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Gao
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D020442:Poloxamer; D011092:Polyethylene Glycols; D011098:Polyglactin 910; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D008823:Micelles; D013778:Tetrazolium Salts; D013844:Thiazoles; C102418:poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer; D020442:Poloxamer; D011098:Polyglactin 910; D011092:Polyethylene Glycols; D004317:Doxorubicin; C022616:thiazolyl blue