Immunocytochemical evidence for the presence of enzymes synthesizing GABA and GHB in the same neuron.
A specific and sensitive immunocytochemical double staining for visualization of glutamate decarboxylase (GAD) and semialdehyde succinate reductase (SSR(2)) in the same brain section has been developed. SSR(2) is the enzyme responsible for the transformation of succinic semialdehyde into ?-hydroxybutyrate (GHB). GAD was detected using specific rabbit GAD-antibodies and unlabeled antibody enzyme peroxidase antiperoxidase, and SSR(2) using specific guinea-pig SSR(2) antibodies conjugate to a fluorescein-labeled second antibody. The coexistence of GAD and SSR(2) in the same neuron was demonstrated by a peroxidase reaction superimposed on fluorescent compounds. Cell bodies containing both antigens were observed in the cerebellum, dorso-median hypothalamus and raphe nuclei. GHB is present in most GABA containing neurons. Some neurons contain only SSR(2); these neurons may synthesize GHB by an active uptake of GABA.
Weissmann-Nanopoulos
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INSERM U44, 5 rue Blaise Pascal, 67084 Strasbourg Cedex, France.
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Mandel
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Maitre
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