title: Light-evoked responses of the retinal pigment epithelium: changes accompanying photoreceptor loss in the mouse.
abstract: Mutations in genes expressed in the retinal pigment epithelium (RPE) underlie a number of human inherited retinal disorders that manifest with photoreceptor degeneration. Because light-evoked responses of the RPE are generated secondary to rod photoreceptor activity, RPE response reductions observed in human patients or animal models may simply reflect decreased photoreceptor input. The purpose of this study was to define how the electrophysiological characteristics of the RPE change when the complement of rod photoreceptors is decreased. To measure RPE function, we used an electroretinogram (dc-ERG)-based technique. We studied a slowly progressive mouse model of photoreceptor degeneration (Prph(Rd2/+)), which was crossed onto a Nyx(nob) background to eliminate the b-wave and most other postreceptoral ERG components. On this background, Prph(Rd2/+) mice display characteristic reductions in a-wave amplitude, which parallel those in slow PIII amplitude and the loss of rod photoreceptors. At 2 and 4 mo of age, the amplitude of each dc-ERG component (c-wave, fast oscillation, light peak, and off response) was larger in Prph(Rd2/+) mice than predicted by rod photoreceptor activity (Rm(P3)) or anatomical analysis. At 4 mo of age, the RPE in Prph(Rd2/+) mice showed several structural abnormalities including vacuoles and swollen, hypertrophic cells. These data demonstrate that insights into RPE function can be gained despite a loss of photoreceptors and structural changes in RPE cells and, moreover, that RPE function can be evaluated in a broader range of mouse models of human retinal disease.
authors.affiliation: Research Service, Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio 44106, USA. samueli@ccf.org
authors.forename: Ivy S
authors.initials: IS
authors.lastname: Samuels
authors.forename: Gwen M
authors.initials: GM
authors.lastname: Sturgill
authors.forename: Gregory H
authors.initials: GH
authors.lastname: Grossman
authors.forename: Mary E
authors.initials: ME
authors.lastname: Rayborn
authors.forename: Joe G
authors.initials: JG
authors.lastname: Hollyfield
authors.forename: Neal S
authors.initials: NS
authors.lastname: Peachey
mesh_terms: D000375:Aging / Q000502:physiology; D000465:Algorithms; D000483:Alleles; D000818:Animals; D004596:Electroretinography; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000235:genetics; D008027:Light; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics; D064531:Peripherins; D012162:Retinal Degeneration / Q000235:genetics / Q000473:pathology; D055213:Retinal Pigment Epithelium / Q000473:pathology / Q000528:radiation effects*; D017948:Retinal Rod Photoreceptor Cells / Q000473:pathology / Q000528:radiation effects*
chemical_list: D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; C578994:PRPH protein, human; D064531:Peripherins; C579005:Prph protein, mouse