[Interferon-inducible genes 2', 5'-ollgoadenylate synthetase-like and tetratricopeptide repeats 2 are correlated with clinical features of patients with systemic lupus erythematosus].
OBJECTIVE
To investigate the expression levels of 2', 5'-oligoadenylate synthetase-like (OASL) and interferon induced protein with tetratricopeptide repeats 2 (IFIT2) genes in the peripheral blood leukocyte of patients with systemic lupus erythematosus (SLE), and to evaluate the relations between these gene expression levels and disease activity.


METHODS
The clinical data of 50 SLE patients, 25 non-SLE patients with rheumatic diseases, and 25 normal controls were collected. Specimens of peripheral blood were collected; total RNA was extracted and transcribed into cDNA. SYBR green dye based real-time quantitative PCR method was applied to compare the expression levels (indicated as deltaCT value) of OASL and IFIT2 in patients with SLE and those in the controls.


RESULTS
The deltaCT value of OASL expression level of the SLE patients was (4.83 +/- 0.41), significantly higher than those of the non-SLE patients (3.26 +/- 0.47) and normal controls (3.07 +/- 0.54, both P < 0.05), The deltaCT value of IFIT2 expression level of the SLE patients was (2.85 +/- 0.41), significantly higher than those of the non-SLE patients (1.19 +/- 0.52) and normal controls (1.07 +/- 0.47, both P < 0.05). The deltaCT value of OASL is related with the SLEDAI scores and immunoglobulin A. (Both P < 0.05). The deltaCT value of IFIT2 is related with the SLEDAI scores and IgA, white blood cell (both P < 0.05).


CONCLUSIONS
The value of IFIT2 and OASL expression level of the SLE patients is up-regulation, the real time expression levels of OASL and IFIT2 genes are associated with SLE disease activity. To inhibit the expression of OASL and IFIT2 may become a novel therapeutic approach for SLE.
Zhao
Zhen-yi
ZY
Department of Rheumatology, West China Hospital, Sichuan University, Chengdu 610041, China.
Yang
Nan-ping
NP
Huang
Xiang-yang
XY
Min
Li
L
Liu
Xiao-xiao
XX
Zhang
Jie
J
Ye
Yang
Y
Shi
Lian-jie
LJ
Zhong
Xue
X
D015088:2',5'-Oligoadenylate Synthetase / Q000097:blood / Q000235:genetics*; D000328:Adult; D051017:Apoptosis Regulatory Proteins; D005260:Female; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D016601:RNA-Binding Proteins; D055815:Young Adult
D051017:Apoptosis Regulatory Proteins; C484289:IFIT2 protein, human; D011506:Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D007372:Interferons; C115020:OASL protein, human; D015088:2',5'-Oligoadenylate Synthetase