title: Identification of an intergenic region that is not essential for replication of goatpox virus.
abstract: A recombinant goatpox virus was constructed in which an enhanced green fluorescent protein gene was inserted under the control of the 11K late promoter, a guanine phosphoribosyltransferase gene was inserted under the control of the 7.5K early/late promoter, and exogenous genes were inserted into an intergenic region between loci gp_24 and gp_24.5 of the recombinant genome. Analysis of protein expression showed that LT cells infected with only the recombinant virus produced specific fluorescence. A comparative growth assay demonstrated the stability of the recombinant virus at the replication level. These results suggest that the intergenic region is not essential for replication of goatpox virus.
authors.affiliation: Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, People's Republic of China. mawenge2004@yahoo.com.cn
authors.forename: Wenge
authors.initials: W
authors.lastname: Ma
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Xue
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.forename: Jiong
authors.initials: J
authors.lastname: Huang
authors.forename: Zihua
authors.initials: Z
authors.lastname: Fu
mesh_terms: D000818:Animals; D018151:Capripoxvirus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D002460:Cell Line; D021901:DNA, Intergenic / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D013737:Testis / Q000166:cytology / Q000821:virology; D014162:Transfection; D014779:Virus Replication
chemical_list: D021901:DNA, Intergenic; D008164:Luminescent Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D007041:Hypoxanthine Phosphoribosyltransferase