title: Glyceraldehyde-3-phosphate dehydrogenase interacts with phosphorylated Akt resulting from increased blood glucose in rat cardiac muscle.
abstract: Here we describe the interaction of phosphorylated approximately 40 kDa protein with phosphorylated Akt which is a serine/threonine kinase resulting from increased blood glucose in rat cardiac muscle. Mass spectrometry analysis revealed that this protein was glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Furthermore, increase in Akt and GAPDH phosporylation and induction of their association were both observed after insulin stimulation in the H9c2 cell line derived from embryonic rat ventricle. Moreover, the activation of GAPDH was upregulated when the GAPDH phosphorylation was increased. Our data suggest that GAPDH phosphorylation and association with Akt by insulin treatment have some bearing on the enhancement of GAPDH activity.
authors.affiliation: Department of Chemistry, Faculty of Medicine, Juntendo University, Inzai-shi, Chiba, Japan. t_baba@sakura.juntendo.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Baba
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Reiko
authors.initials: R
authors.lastname: Mineki
authors.forename: Hisashi
authors.initials: H
authors.lastname: Naito
authors.forename: Daijiro
authors.initials: D
authors.lastname: Ohmori
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002460:Cell Line; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D015151:Immunoblotting; D047468:Immunoprecipitation; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D007328:Insulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D051057:Proto-Oncogene Proteins c-akt