On the kinetics of cholinergic excitation.
We have studied the interaction of NBD-n-acylcholines, fluorescent analogs of acetylcholine with agonist action, with acetylcholine receptor and acetylcholine esterase (EC 3.1.1.7) from Electrophorus electricus. The rates of association of the fluorescent ligands with the two proteins are closely similar during the initial phase of binding, the second order rate constants being of the order of 2 x 10(8) M(?1) s(?1) at physiological salt conditions.
Prinz
H
H
Max-Planck-Institut, Rheinlanddamm 201, 4600 Dortmund, West-Germany.
Jürss
R
R
Maelicke
A
A