title: A series of fluorene-based two-photon absorbing molecules: synthesis, linear and nonlinear characterization, and bioimaging.
abstract: The synthesis, structural, and photophysical characterization of a series of new fluorescent donor-acceptor and acceptor-acceptor molecules, based on the fluorenyl ring system, with two-photon absorbing properties is described. These new compounds exhibited large Stokes shifts, high fluorescence quantum yields, and significantly, high two-photon absorption cross sections, making them well suited for two-photon fluorescence microscopy imaging. Confocal and two-photon fluorescence microscopy imaging of COS-7 and HCT 116 cells incubated with probe I showed endosomal selectivity, demonstrating the potential of this class of fluorescent probes in multiphoton fluorescence microscopy.
authors.affiliation: Department of Chemistry, University of Central Florida, Orlando, Florida 32816, USA.
authors.forename: Carolina D
authors.initials: CD
authors.lastname: Andrade
authors.forename: Ciceron O
authors.initials: CO
authors.lastname: Yanez
authors.forename: Luis
authors.initials: L
authors.lastname: Rodriguez
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Belfield
mesh_terms: D000042:Absorption; D005449:Fluorenes / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D045325:HCT116 Cells; D006801:Humans; D018613:Microscopy, Confocal; D057054:Molecular Imaging; D015394:Molecular Structure; D010777:Photochemistry; D017785:Photons
chemical_list: D005449:Fluorenes; D005456:Fluorescent Dyes