title: Transgenic expression of trehalulose synthase results in high concentrations of the sucrose isomer trehalulose in mature stems of field-grown sugarcane.
abstract: Sugarcane plants were developed that produce the sucrose isomers trehalulose and isomaltulose through expression of a vacuole-targeted trehalulose synthase modified from the gene in 'Pseudomonas mesoacidophila MX-45' and controlled by the maize ubiquitin (Ubi-1) promoter. Trehalulose concentration in juice increased with internode maturity, reaching about 600 mM, with near-complete conversion of sucrose in the most mature internodes. Plants remained vigorous, and trehalulose production in selected lines was retained over multiple vegetative generations under glasshouse and field conditions.
authors.affiliation: Botany Department/BIOL, The University of Queensland, Brisbane, Qld, Australia.
authors.forename: Dénes
authors.initials: D
authors.lastname: Hamerli
authors.forename: Robert G
authors.initials: RG
authors.lastname: Birch
mesh_terms: D004187:Disaccharides / Q000096:biosynthesis*; D019751:Intramolecular Transferases / Q000235:genetics* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000235:genetics; D031786:Saccharum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D025822:Ubiquitin C / Q000235:genetics / Q000378:metabolism; D014617:Vacuoles / Q000201:enzymology; D003313:Zea mays / Q000235:genetics
chemical_list: D004187:Disaccharides; D010940:Plant Proteins; C417884:Ubi-1 protein, Zea mays; D025822:Ubiquitin C; D019751:Intramolecular Transferases; C042002:isomaltulose synthase; C042003:trehalulose