Intramolecular interactions between the protease and structural domains are important for the functions of serine protease autotransporters.
Autotransporter (AT) is a protein secretion pathway found in Gram-negative bacteria featuring a multidomain polypeptide with a signal sequence, a passenger domain, and a translocator domain. An AT subfamily named serine protease ATs of the family Enterobacteriaceae (SPATEs) is characterized by the presence of a conserved serine protease motif in the passenger domain which contributes to bacterial pathogenesis. The goal of the current study is to determine the importance of the passenger domain conserved residues in the SPATE proteolytic and adhesive functions using the temperature-sensitive hemagglutinin (Tsh) protein as our model. To begin, mutations of 21 fully conserved residues in the four passenger domain conserved motifs were constructed by PCR-based site-directed mutagenesis. Seventeen mutants exhibited a wild-type secretion level; among these mutants, eight displayed reduced proteolytic activities in Tsh-specific oligopeptide and mucin cleavage assays. These eight mutants also demonstrated lower affinities to extracellular matrix proteins, collagen IV, and fibronectin. These eight conserved residues were analyzed by molecular graphics modeling to demonstrate their intramolecular interactions with the catalytic triad and other key residues. Additional mutations were made to confirm the above interactions in order to demonstrate their significance to the SPATE functions. Altogether our data suggest that certain conserved residues in the SPATE passenger domain are important for both the proteolytic and adhesive activities of SPATE by maintaining the proper protein structure via intramolecular interactions between the protease and beta-helical domains. Here, we provide new insight into the structure-function relationship of the SPATEs and the functional roles of their conserved residues.
Tsang
Casey
C
Department of Biological Sciences, California State Polytechnic University, Pomona, CA 91709, USA.
Malik
Huma
H
Nassman
Deana
D
Huang
Antony
A
Tariq
Fayha
F
Oelschlaeger
Peter
P
Stathopoulos
Christos
C
D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D024141:Collagen Type IV / Q000378:metabolism; D004755:Enterobacteriaceae / Q000201:enzymology*; D005353:Fibronectins / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D009077:Mucins / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D009842:Oligopeptides / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D057057:Serine Proteases / Q000235:genetics / Q000378:metabolism*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
D018830:Adhesins, Escherichia coli; D001426:Bacterial Proteins; D024141:Collagen Type IV; D005353:Fibronectins; D026901:Membrane Transport Proteins; D009077:Mucins; D009842:Oligopeptides; D037521:Virulence Factors; C112027:tsh protein, E coli; D057057:Serine Proteases