title: The XC chemokine receptor 1 is a conserved selective marker of mammalian cells homologous to mouse CD8alpha+ dendritic cells.
abstract: Human BDCA3+ dendritic cells (DCs) were suggested to be homologous to mouse CD8alpha+ DCs. We demonstrate that human BDCA3+ DCs are more efficient than their BDCA1+ counterparts or plasmacytoid DCs (pDCs) in cross-presenting antigen and activating CD8+ T cells, which is similar to mouse CD8alpha+ DCs as compared with CD11b+ DCs or pDCs, although with more moderate differences between human DC subsets. Yet, no specific marker was known to be shared between homologous DC subsets across species. We found that XC chemokine receptor 1 (XCR1) is specifically expressed and active in mouse CD8alpha+, human BDCA3+, and sheep CD26+ DCs and is conserved across species. The mRNA encoding the XCR1 ligand chemokine (C motif) ligand 1 (XCL1) is selectively expressed in natural killer (NK) and CD8+ T lymphocytes at steady-state and is enhanced upon activation. Moreover, the Xcl1 mRNA is selectively expressed at high levels in central memory compared with naive CD8+ T lymphocytes. Finally, XCR1-/- mice have decreased early CD8+ T cell responses to Listeria monocytogenes infection, which is associated with higher bacterial loads early in infection. Therefore, XCR1 constitutes the first conserved specific marker for cell subsets homologous to mouse CD8alpha+ DCs in higher vertebrates and promotes their ability to activate early CD8+ T cell defenses against an intracellular pathogenic bacteria.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Parc scientifique et technologique de Luminy, 13288 Marseille, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Crozat
authors.forename: Rachel
authors.initials: R
authors.lastname: Guiton
authors.forename: Vanessa
authors.initials: V
authors.lastname: Contreras
authors.forename: Vincent
authors.initials: V
authors.lastname: Feuillet
authors.forename: Charles-Antoine
authors.initials: CA
authors.lastname: Dutertre
authors.forename: Erwan
authors.initials: E
authors.lastname: Ventre
authors.forename: Thien-Phong
authors.initials: TP
authors.lastname: Vu Manh
authors.forename: Thomas
authors.initials: T
authors.lastname: Baranek
authors.forename: Anne K
authors.initials: AK
authors.lastname: Storset
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Marvel
authors.forename: Pierre
authors.initials: P
authors.lastname: Boudinot
authors.forename: Anne
authors.initials: A
authors.lastname: Hosmalin
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schwartz-Cornil
authors.forename: Marc
authors.initials: M
authors.lastname: Dalod
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000382:microbiology; D019744:Chemokines, C / Q000235:genetics / Q000378:metabolism; D017124:Conserved Sequence; D045142:Cross-Priming / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology; D008088:Listeriosis / Q000276:immunology / Q000382:microbiology; D008322:Mammals / Q000276:immunology*; D051379:Mice; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000276:immunology*; D012756:Sheep; D018180:Thrombomodulin
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D019744:Chemokines, C; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C546931:THBD protein, human; D018180:Thrombomodulin; C092390:XCL1 protein, human; D018819:Dipeptidyl Peptidase 4