Nucleotide and deduced amino acid sequences of the GABA(A) receptor ?-subunit from human brain.
cDNA cloning of the ?-aminobutyric acid (GABA)(A) receptor ?-subunit from human brain has been attempted in order to clarify its structure. Using a bovine cDNA fragment as a homologous probe, the cDNA clone containing complete coding regions of the GABA(A) receptor ?-subunit was isolated by screening the human brain cDNA library, and its full amino acid sequences were deduced from the nucleotide sequence. The nucleotide and deduced amino acid sequences showed a high homology in comparison with those in bovine brain. The human brain mRNA indicated the presence of a single mRNA species of about 4500 bases by RNA blot hybridization. In addition, Southern blot analysis suggested that the human gene of the GABA(A) receptor ?-subunit might possess an exon-intron organization.
Hirouchi
M
M
Department of Pharmacology, Kyoto Prefectural University of Medicine, Kyoto 602, Japan.
Kuwano
R
R
Takashi
K
K
Yasuo
T
T
Kuriyama
K
K