Expression of the zebrafish CD133/prominin1 genes in cellular proliferation zones in the embryonic central nervous system and sensory organs.
The CD133/prominin1 gene encodes a pentamembrane glycoprotein cell surface marker that is expressed in stem cells from neuroepithelial, hematopoietic, and various organ tissues. Here we report the analysis of two zebrafish CD133/prominin1 orthologues, prominin1a and prominin1b. The expression patterns of the zebrafish prominin1a and b genes were analyzed during embryogenesis using whole mount in situ hybridization. prominin1a and b show novel complementary and overlapping patterns of expression in proliferating zones in the developing sensory organs and central nervous system. The expression patterns suggest functional conservation of the zebrafish prominin1 genes. Initial analyses of prominin1a and b in neoplastic tissue show increased expression of both genes in a subpopulation of cells in malignant peripheral nerve sheath tumors in tp53 mutants. Based on these analyses, the zebrafish prominin1 genes will be useful markers for examining proliferating cell populations in adult organs, tissues, and tumors.
McGrail
Maura
M
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA. mmcgrail@iastate.edu
Batz
Lindsey
L
Noack
Kristin
K
Pandey
Saumya
S
Huang
Yong
Y
Gu
Xun
X
Essner
Jeffrey J
JJ
D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD; D049109:Cell Proliferation; D002490:Central Nervous System / Q000196:embryology* / Q000378:metabolism*; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics; D005796:Genes; D006023:Glycoproteins; D010455:Peptides; D012679:Sense Organs / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D010455:Peptides