[Antioxidants inhibit the oxidative modification of high density lipoproteins].
OBJECTIVE
To study the role and mechanism of antioxidants on inhibiting oxidative modification of high density lipoproteins (HDL).


METHODS
Freshly prepared human plasma HDL was treated by incubation with copper ion, hyperchlorite or arterial wall cells. Compared to control, the test groups were treated with addition of different concentration of butylhydroxytoluene (BHT), vitamin C and vitamin E. Then, the relative electrophoretic mobility (REM), thiobarbituric acid-reactive substances (TBARS), ratio of lysolecithin to lecithin (LPC/PC), and lipoprotein moieties were investigated.


RESULTS
BHT, vitamin C and vitamin E can significantly inhibit the increasing REM, TBARS, LPC/PC ratio and lipoprotein variation that induced by copper ion and hyperchlorite and arterial wall cells. But these antioxidants act on different manner.


CONCLUSIONS
BHT, vitamin C and vitamin E can inhibit the oxidative modification of HDL and hence could be potential nutrients to prevent atherosclerosis.
Li
Xiao-ying
XY
Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Xu
Fei
F
Xing
Rui-qing
RQ
Li
Jun-hong
JH
Liu
Bing-wen
BW
Fu
Ming-de
MD
Fu
Qiang
Q
D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D003300:Copper / Q000633:toxicity*; D006801:Humans; D054709:Lecithins / Q000032:analysis; D008075:Lipoproteins, HDL / Q000737:chemistry* / Q000187:drug effects*; D008244:Lysophosphatidylcholines / Q000032:analysis; D010084:Oxidation-Reduction / Q000187:drug effects; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D054709:Lecithins; D008075:Lipoproteins, HDL; D008244:Lysophosphatidylcholines; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; D003300:Copper; D001205:Ascorbic Acid