Selected fluorescent techniques for identification of the physiological state of individual water and soil bacterial cells - review.
Stimulated by demands of the natural environment conservation, the need for thorough structural and functional identification of microorganisms colonizing different ecosystems has contributed to an intensive advance in research techniques. The article shows that some of these techniques are also a convenient tool for determination of the physiological state of single cells in a community of microorganisms. The paper presents selected fluorescent techniques, which are used in research on soil, water and sediment microorganisms. It covers the usability of determination of the dehydrogenase activity of an individual bacterial cell (CTC+) and of bacteria with intact, functioning cytoplasmic membranes, bacteria with an integrated nucleiod (NuCC+) as well as fluorescent in situ hybridization (FISH).
Lew
S
S
Department of Microbiology, Faculty of Biology, University of Warmia and Mazury, 10-717, Olsztyn, Poland. sylwia.lew@uwm.edu.pl
Lew
M
M
Mieszczyński
T
T
Szarek
J
J
D001419:Bacteria / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques / Q000379:methods; D005470:Fluorometry / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence; D012988:Soil Microbiology; D014871:Water Microbiology
D001426:Bacterial Proteins