title: Potential for the Anopheles gambiae densonucleosis virus to act as an "evolution-proof" biopesticide.
abstract: "Evolution-proof" or "late-life-acting" insecticides (LLAIs) preferentially kill older adult mosquitoes and are of extreme interest to control vector-borne diseases such as malaria. We used quantitative PCR to assess whether the Anopheles gambiae densonucleosis virus (AgDNV) had potential as an LLAI. After infection, AgDNV titers increased modestly during larval development but replicated slower than the host cells, resulting in a significant decrease in the normalized virus titer during larval and pupal development. Normalized virus titers dramatically increased after adult emergence, peaking in 7- to 10-day-old adults. Unlike other DNVs, AgDNV does not significantly replicate in preadult mosquitoes but rather preferentially replicates in older adults. The natural dynamics of AgDNV make it ideal for expression of insect-specific toxin genes as a biological LLAI.
authors.affiliation: W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Ren
authors.forename: Jason L
authors.initials: JL
authors.lastname: Rasgon
mesh_terms: D000818:Animals; D000852:Anopheles / Q000821:virology*; D004279:DNA, Viral / Q000096:biosynthesis; D019223:Densovirinae / Q000472:pathogenicity*; D007814:Larva / Q000821:virology; D010572:Pest Control, Biological / Q000379:methods*; D016133:Polymerase Chain Reaction; D019562:Viral Load
chemical_list: D004279:DNA, Viral