Parasite excretory-secretory proteins elicit TRIF dependent CXCL1 and IL-6 mediated allergic inflammation.
Currently, little information is available regarding innate immunity to helminthic parasite infection. In this study, we isolated the excretory-secretory (ES) proteins from Anisakis simplex (sea mammal intestinal parasite) third stage larva. We determined that the levels of IL-17 in the lung and lung draining lymph node of mice were increased sixfold as a result of intranasal treatment with ES proteins. The ES protein treatment elicited pro-inflammatory cytokine and chemokine secretion (especially IL-6 and CXCL1) from mouse lung epithelial cell line and primary lung epithelial cells. In addition, the expression of IL-6 and CXCL1 in mouse embryonic fibroblast (MEF) cells was significantly increased by the ES protein treatment, but we did not detect these effects in the TRIF(-/-) MEF cells. These elevations of IL-6 and CXCL1 expression were also not diminished by RNase treatment. In conclusion, the ES proteins of helminthic parasite larva may elicit TRIF dependent pro-inflammatory cytokines, and this is not double-stranded RNA.
Cho
M K
MK
Department of Parasitology, School of Medicine, Pusan National University, Gyeongsangnam-do, Korea.
Ahn
S C
SC
Kim
D-H
DH
Yu
H S
HS
D033942:Adaptor Proteins, Vesicular Transport / Q000172:deficiency / Q000276:immunology*; D000818:Animals; D017163:Anisakis / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology; D002478:Cells, Cultured; D054360:Chemokine CXCL1 / Q000276:immunology*; D004847:Epithelial Cells / Q000276:immunology; D015801:Helminth Proteins / Q000276:immunology*; D006967:Hypersensitivity / Q000473:pathology*; D007249:Inflammation / Q000276:immunology*; D020381:Interleukin-17 / Q000032:analysis; D015850:Interleukin-6 / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout
D033942:Adaptor Proteins, Vesicular Transport; D000947:Antigens, Helminth; D054360:Chemokine CXCL1; D015801:Helminth Proteins; D020381:Interleukin-17; D015850:Interleukin-6; C475895:Cxcl1 protein, mouse; C476286:Ticam1 protein, mouse