Expression of TNF-alpha and IL-6 in HMC-1 cells treated with bisphenol A is attenuated by plant-originating glycoprotein (75 kDa) by blocking p38 MAPK.
Bisphenol A (BPA) is known as an estrogen-mimic environmental hormone which has the ability to indirectly stimulate the production of allergic inflammation-related cytokines. Cudrania tricuspidata Bureau (CTB) has been used in Korean folk medicine for a long time. In order to determine the inhibitory effect of a glycoprotein (CTB glycoprotein, 75 kDa) isolated from CTB fruits on the activities of allergic inflammation-related cytokines (TNF-alpha and IL-6) caused by BPA, we evaluated the activities of protein kinase C (PKC), p38 mitogen-activated protein kinase (p38 MAPK), nuclear factor (NF)-kappaB, and inflammation-related cytokine (TNF-alpha and IL-6) in the BPA-induced HMC-1 cells using immunoblot analysis and RT-PCR. The results obtained from this study revealed that CTB glycoprotein (100 microg/ml) inhibits the translocation of PKC from cytosol to the membrane, the phosphorylation of p38 MAPK, the activation of NF-kappaB, and the expression levels of TNF-alpha and IL-6. Taken together, the results in this study suggest that CTB glycoprotein inhibits the expression of allergic inflammation-related cytokines (TNF-alpha and IL-6) by blocking NF-kappaB and p38 kinase in BPA-induced HMC-1 cells.
Lee
Jin
J
Molecular Biochemistry Laboratory, Biotechnology Research Institute & Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, 300 Yongbong-Dong, Gwang-ju 500-757, South Korea.
Lim
Kye-Taek
KT
D001559:Benzhydryl Compounds; D002460:Cell Line; D005638:Fruit; D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D015151:Immunoblotting; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D055670:Medicine, Korean Traditional; D029586:Moraceae / Q000737:chemistry; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D010636:Phenols / Q000633:toxicity*; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D006023:Glycoproteins; D015850:Interleukin-6; D016328:NF-kappa B; D010636:Phenols; D011493:Protein Kinase C; D014409:Tumor Necrosis Factor-alpha; D048051:p38 Mitogen-Activated Protein Kinases; C006780:bisphenol A; D000098826:Bisphenol A Compounds