title: Real-time reaction monitoring by probe electrospray ionization mass spectrometry.
abstract: Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study, PESI-MS was used to monitor some biological and chemical reactions in real-time, such as acid-induced protein denaturation, hydrogen/deuterium exchange (HDX) of peptides, and Schiff base formation. By using PESI-MS, time-resolved mass spectra and ion chromatograms can be obtained reproducibly. Real-time PESI-MS monitoring can give direct and detailed information on each chemical species taking part in reactions, and this is valuable for a better understanding of the whole reaction process and for the optimization of reaction parameters. PESI-MS can be considered as a potential tool for real-time reaction monitoring due to its simplicity in instrumental setup, direct sampling with minimum sample preparation and low sample consumption.
authors.affiliation: Clean Energy Research Center, University of Yamanashi, Kofu, Japan.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Yu
authors.forename: Lee Chuin
authors.initials: LC
authors.lastname: Chen
authors.forename: Rosa
authors.initials: R
authors.lastname: Erra-Balsells
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nonami
authors.forename: Kenzo
authors.initials: K
authors.lastname: Hiraoka
mesh_terms: D055598:Chemical Phenomena; D003199:Computer Systems; D006801:Humans; D015394:Molecular Structure; D009211:Myoglobin / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011489:Protein Denaturation; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D009211:Myoglobin; D010455:Peptides