title: Paradoxical ATP elevation in ischemic penumbra revealed by quantitative imaging mass spectrometry.
abstract: Local responses of energy metabolism during brain ischemia are too heterogeneous to decipher redox distribution between anoxic core and adjacent salvageable regions such as penumbra. Imaging mass spectrometry combined by capillary electrophoresis/mass spectrometry providing quantitative metabolomics revealed spatio-temporal changes in adenylates and NADH in a mouse middle-cerebral artery occlusion model. Unlike the core where ATP decreased, the penumbra displayed paradoxical elevation of ATP despite the constrained blood supply. It is noteworthy that the NADH elevation in the ischemic region is clearly demarcated by the ATP-depleting core. Results suggest that metabolism in ischemic penumbra does not respond passively to compromised circulation, but actively compensates energy charges.
authors.affiliation: Department of Biochemistry, School of Medicine, Keio University, Shinjuku-ku, Tokyo, Japan.
authors.forename: Katsuji
authors.initials: K
authors.lastname: Hattori
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kajimura
authors.forename: Takako
authors.initials: T
authors.lastname: Hishiki
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Akiko
authors.initials: A
authors.lastname: Kubo
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Nagahata
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Ohmura
authors.forename: Ayako
authors.initials: A
authors.lastname: Yachie-Kinoshita
authors.forename: Tomomi
authors.initials: T
authors.lastname: Matsuura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Morikawa
authors.forename: Tomomi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
authors.forename: Makoto
authors.initials: M
authors.lastname: Suematsu
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002545:Brain Ischemia / Q000175:diagnosis / Q000378:metabolism* / Q000503:physiopathology; D002540:Cerebral Cortex / Q000098:blood supply / Q000503:physiopathology; D004195:Disease Models, Animal; D019075:Electrophoresis, Capillary; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism / Q000503:physiopathology; D013058:Mass Spectrometry; D051379:Mice; D010084:Oxidation-Reduction; D015398:Signal Transduction
chemical_list: D000255:Adenosine Triphosphate