title: Stimulation by glia maturation factor of Ca(2+)-dependent phosphorylation of M(r) 100 k protein in rat glioblasts.
abstract: When quiescent rat glioblasts were stimulated by glia maturation factor (GMF), their intrinsic Ca(2+)-dependent phosphorylation of proteins, especially that of M(r) 100 k protein, increased. The phosphorylation of M(r) 100 k protein in the homogenate started rising 13 h (S phase) after GMF stimulation and reached the maximal level (8-fold greater than the control) at 26 h. Phosphorylation was also detected in intact cells by the use of [(32)P]orthophosphate. Calmodulin augmented and W-7 (calmodulin inhibitor) slightly inhibited the phosphorylation, suggesting that Ca(2+)/calmodulin-dependent protein kinase may partly be involved in phosphorylation of the M(r) 100 k protein. Subcellular fractionation experiments revealed that both M(r) 100 k protein and its kinase were localized exclusively in the cytosol. We also found marked phosphorylation of M(r) 100 k protein in neural tumor cell lines, mouse neuroblastoma (Neuro2a and NAs-1) and glioma (C6 and 354A). Since the peptide maps of (32)P-labeled peptides obtained by chemical cleavage from M(r) 100 k protein of the cells were identical to those of glioblasts, the M(r) 100 k proteins, regardless of cell origin, may be closely related in structure. Growth inhibitors, W-7 (50 ?M), puromucin (2 ?M), spongoadenosine (50 ?M), diphenylhydantoin (0.3 mM), ?-sialosyl cholesterol (20 ?g/ml) and protein kinase inhibitor, K252a (50 nM), lowered the phosphorylation of the M(r) 100 k protein in the cell homogenate derived from glioblasts pretreated with the drugs for 24 h. M(r) 100 k protein of glioblasts and C6 cells was immunoprecipitated by anti-elongation factor-2 (EF-2) antiserum indicating an identity or similarity in structure between the protein and EF-2. These findings provide a possibility that cell growth may be brought about through a phosphorylation of M(r) 100 k protein as one of the signal transduction processes subsequent to a mitogen stimulation.
authors.affiliation: Department of Biochemistry, Nagoya City University Medical School, Mizuho-ku, Nagoya 467, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Okumura-Noji
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: J
authors.initials: J
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: R
authors.initials: R
authors.lastname: Tanaka