[The effect of CCL19 on the viability of head and neck squamous cancer cells].
OBJECTIVE
To study the role of CCL19 on the viability,cell-cycle and apoptosis of head and neck squamous cancer cells.


METHODS
We assayed the growth arrest induced by cisplatin in 4A cells and 4B cells by MTT, detected the role of CCL19 on the effect of cisplatin and observed the change of morphology by transmission electron microscope, evaluated cell-cycle and apoptosis of 4B cell pretreated by CCL19 and cisplatin via FCM. Statistical differences between the two groups were evaluated using the unpaired Student's t test. All statistical analyses were performed with SPSS 11.0 software package.


RESULTS
The growth arrest of 4B cell by cisplatin was significantly higher than 4A(P<0.05). CCL19 could protect 4B cell against inhibition, but no effect on 4A. CCL19 contributed to 4B by promoting to normal morphology, reducing G1 phase arrest, inhibiting apoptosis.


CONCLUSIONS
Cisplatin works better in 4B cell than in 4A cell, and CCL19 can inhibit its effect, which provide a new way to treat head and neck squamous cancer. Supported by National Natural Science Foundation of China (Grant No.30672331) and Foundation of Liaoning Provincial Educational Commission (Grant No.2009A755).
Liu
Fa-yu
FY
Oromaxillofacial Head and Neck Tumor Center, China Medical University, Shenyang 110002, Liaoning Province, China. lfyhjk@126.com
Zhao
Zhen-jin
ZJ
Li
Peng
P
Ding
Xue
X
Sun
Chang-fu
CF
D000970:Antineoplastic Agents; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell; D002945:Cisplatin; D006258:Head and Neck Neoplasms; D006801:Humans; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D002945:Cisplatin