Does abscisic acid affect strigolactone biosynthesis?
*Strigolactones are considered a novel class of plant hormones that, in addition to their endogenous signalling function, are exuded into the rhizosphere acting as a signal to stimulate hyphal branching of arbuscular mycorrhizal (AM) fungi and germination of root parasitic plant seeds. Considering the importance of the strigolactones and their biosynthetic origin (from carotenoids), we investigated the relationship with the plant hormone abscisic acid (ABA). *Strigolactone production and ABA content in the presence of specific inhibitors of oxidative carotenoid cleavage enzymes and in several tomato ABA-deficient mutants were analysed by LC-MS/MS. In addition, the expression of two genes involved in strigolactone biosynthesis was studied. *The carotenoid cleavage dioxygenase (CCD) inhibitor D2 reduced strigolactone but not ABA content of roots. However, in abamineSG-treated plants, an inhibitor of 9-cis-epoxycarotenoid dioxygenase (NCED), and the ABA mutants notabilis, sitiens and flacca, ABA and strigolactones were greatly reduced. The reduction in strigolactone production correlated with the downregulation of LeCCD7 and LeCCD8 genes in all three mutants. *The results show a correlation between ABA levels and strigolactone production, and suggest a role for ABA in the regulation of strigolactone biosynthesis.
López-Ráez
Juan A
JA
Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.|Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
Kohlen
Wouter
W
Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.
Charnikhova
Tatsiana
T
Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.
Mulder
Patrick
P
RIKILT, Institute of Food Safety, Bornsesteeg 45, NL-6708 PD Wageningen, the Netherlands.
Undas
Anna K
AK
Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.|Centre for Biosystems Genomics, PO Box 98, NL-6700 AB Wageningen, the Netherlands.
Sergeant
Martin J
MJ
Warwick-HRI, Wellesbourne, University of Warwick, Warwickshire, CV35 9EF, UK.
Verstappen
Francel
F
Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.|Centre for Biosystems Genomics, PO Box 98, NL-6700 AB Wageningen, the Netherlands.
Bugg
Timothy D H
TDH
Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK.
Thompson
Andrew J
AJ
Warwick-HRI, Wellesbourne, University of Warwick, Warwickshire, CV35 9EF, UK.
Ruyter-Spira
Carolien
C
Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.
Bouwmeester
Harro
H
Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.|Centre for Biosystems Genomics, PO Box 98, NL-6700 AB Wageningen, the Netherlands.
D000040:Abscisic Acid / Q000096:biosynthesis / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000187:drug effects; D002338:Carotenoids / Q000378:metabolism; D002853:Chromatography, Liquid; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D018525:Germination / Q000187:drug effects; D007783:Lactones / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D009154:Mutation / Q000235:genetics; D031672:Orobanche / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010710:Phosphates / Q000172:deficiency / Q000378:metabolism; D053147:Plant Exudates / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004791:Enzyme Inhibitors; D007783:Lactones; D010710:Phosphates; D053147:Plant Exudates; D002338:Carotenoids; D000040:Abscisic Acid