Corynebacterium humireducens sp. nov., an alkaliphilic, humic acid-reducing bacterium isolated from a microbial fuel cell.
A novel halotolerant, alkaliphilic, humic acid-reducing bacterium, designated MFC-5(T), was isolated from a microbial fuel cell that was fed continuously with artificial wastewater (pH 10.0). Cells were Gram-positive-staining, facultatively anaerobic, non-fermentative, non-motile rods and had a G+C content of 59.0 mol%. Microbial growth was observed with <13 % (w/v) NaCl (optimum 10 %), at pH 7.0-11.0 (optimum pH 9.0) and at 25-45 °C (optimum 37 °C). Strain MFC-5(T) was active in the anaerobic reduction of a humic acid analogue, anthraquinone-2,6-disulphonate, with lactate, formate, acetate, ethanol or sucrose as the electron donor. The major cellular fatty acids were C(18 : 1)ω9c (42.68 %), C(16 : 0) (33.69 %), C(18 : 0) (7.56 %), C(17 : 1)ω8c (5.14 %) and C(17 : 0) (3.39 %). Phylogenetic analysis demonstrated that strain MFC-5(T) displayed >3 % 16S rRNA gene sequence divergence from its closest relatives. Based on phenotypic, genetic and phylogenetic analysis, a novel species, Corynebacterium humireducens sp. nov., is proposed. The type strain is MFC-5(T) ( = NBRC 106098(T)  = CGMCC 2452(T)  = DSM 45392(T)).
Wu
Chun-Yuan
CY
Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, PR China.|Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
Zhuang
Li
L
Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
Zhou
Shun-Gui
SG
Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
Li
Fang-Bai
FB
Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
He
Jian
J
Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
D000693:Anaerobiosis; D000880:Anthraquinones / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D016000:Cluster Analysis; D003352:Corynebacterium / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
D000880:Anthraquinones; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; C408124:anthraquinone-2,6-disulfonate; D012965:Sodium Chloride