Heterogeneity in ventricular zone neural precursors contributes to neuronal fate diversity in the postnatal neocortex.
The recent discovery of short neural precursors (SNPs) in the murine neocortical ventricular zone (VZ) challenges the widely held view that radial glial cells (RGCs) are the sole occupants of this germinal compartment and suggests that precursor variety is an important factor of brain development. Here, we use in utero electroporation and genetic fate mapping to show that SNPs and RGCs cohabit the VZ but display different cell cycle kinetics and generate phenotypically different progeny. In addition, we find that RGC progeny undergo additional rounds of cell division as intermediate progenitor cells (IPCs), whereas SNP progeny generally produce postmitotic neurons directly from the VZ. By clearly defining SNPs as bona fide VZ residents, separate from both RGCs and IPCs, and uncovering their unique proliferative and lineage properties, these results demonstrate how individual neural precursor groups in the embryonic rodent VZ create diversity in the overlying neocortex.
Stancik
Elizabeth K
EK
Center for Neuroscience Research, Children's National Medical Center, Washington, DC 20010, USA.
Navarro-Quiroga
Ivan
I
Sellke
Robert
R
Haydar
Tarik F
TF
D027322:Amino Acid Transport System X-AG / Q000235:genetics; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology*; D002552:Cerebral Ventricles / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D018274:Electroporation / Q000379:methods; D005136:Eye Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D019579:Neocortex / Q000166:cytology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D000071841:PAX6 Transcription Factor; D050809:POU Domain Factors / Q000378:metabolism; D051761:Paired Box Transcription Factors / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D013234:Stem Cells / Q000502:physiology*; D020825:T-Box Domain Proteins / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D000097573:Red Fluorescent Protein
D027322:Amino Acid Transport System X-AG; D001973:Bromodeoxyuridine; D005136:Eye Proteins; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins; D019394:Ki-67 Antigen; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; D000071841:PAX6 Transcription Factor; D050809:POU Domain Factors; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D020825:T-Box Domain Proteins; D014404:Tubulin; D000097573:Red Fluorescent Protein; C491198:Eomes protein, mouse; C000605732:Pax6 protein, mouse; C462941:beta3 tubulin, mouse; C074065:Pou3f3 protein, mouse