Detection of Salmonella in Swine fecal samples by flow-through immunocapture.
This study reports the preliminary evaluation of flow-through immunocapture (FTI) followed by real-time PCR (FTI-PCR) for the detection of different serotypes of Salmonella in pig fecal specimens. The FTI-PCR method was compared with the direct plating of FTI beads on xylose lysine desoxycholate agar, real-time PCR, and the conventional culturing methods, Rappaport-Vassiliadis and modified semisolid Rappaport-Vassiliadis. Artificially contaminated swine fecal specimens were used to evaluate and compare these methods. The results of our comparisons indicate that the FTI-PCR, FTI-plating, and modified semisolid Rappaport-Vassiliadis culture methods were accurate, specific, and sensitive. Moreover, FTI-PCR was the fastest method, providing results in fewer than 20 h, as opposed to the 48 to 96 h required for the other methods. Our results indicate that FTI-PCR could be a useful tool for detecting Salmonella in swine fecal specimens at a lower limit of 1.0 CFU/g.
Katsuda
Ken
K
Tohoku Research Station, Environmental/Enzootic Diseases Research Team, National Institute of Animal Health, 31 Uminai, Shichinohe, Kamikita, Aomori 039-2586, Japan. katsuda@affrc.go.jp
Kohmoto
Mariko
M
Mikami
Osamu
O
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis*; D005243:Feces / Q000382:microbiology*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012475:Salmonella / Q000302:isolation & purification*; D013552:Swine
D004269:DNA, Bacterial