A unique protein labeling system based on melittin and the non-covalent  binding-induced pyrene excimer.
We report a unique protein labeling system based on melittin and a pyrene derivative (1). The specific region of the C-terminal in melittin efficiently induced the formation of the pyrene eximer, which can be used as a tag to target proteins and for further detection.
Li
Yue
Y
State Key Laboratory of Supramolecular Structure and Materials, Jilin University, No. 2699, Qianjin Street, Changchun, 130012, China.
Li
Hong-Wei
HW
Ma
Li-Jun
LJ
Dang
Yong-Qiang
YQ
Wu
Yuqing
Y
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D008555:Melitten / Q000737:chemistry*; D008969:Molecular Sequence Data; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods*; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000737:chemistry
C551637:N-(4-(1-pyrene)butyroyl)tryptophan; D011721:Pyrenes; D008555:Melitten; D014364:Tryptophan