title: C-type starch from high-amylose rice resistant starch granules modified by antisense RNA inhibition of starch branching enzyme.
abstract: High-amylose starch is a source of resistant starch (RS) which has a great benefit on human health. A transgenic rice line (TRS) enriched amylose and RS had been developed by antisense RNA inhibition of starch branching enzymes. In this study, the native starch granules were isolated from TRS grains as well as the wild type, and their crystalline type was carefully investigated before and after acid hydrolysis. In high-amylose TRS rice, the C-type starch, which might result from the combination of both A-type and B-type starch, was observed and subsequently confirmed by multiple physical techniques, including X-ray powder diffraction, solid-state nuclear magnetic resonance, and Fourier transform infrared. Moreover, the change of starch crystalline structure from C- to B-type during acid hydrolysis was also observed in this RS-rich rice. These data could add to our understanding of not only the polymorph structure of cereal starch but also why high-amylose starch is more resistant to digestion.
authors.affiliation: Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, Yangzhou, China. yzuwcx@yahoo.com.cn
authors.forename: Cunxu
authors.initials: C
authors.lastname: Wei
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Fengling
authors.initials: F
authors.lastname: Qin
authors.forename: Huaguang
authors.initials: H
authors.lastname: Yu
authors.forename: Chong
authors.initials: C
authors.lastname: Chen
authors.forename: Xianglen
authors.initials: X
authors.lastname: Meng
authors.forename: Lijia
authors.initials: L
authors.lastname: Zhu
authors.forename: Youping
authors.initials: Y
authors.lastname: Wang
authors.forename: Minghong
authors.initials: M
authors.lastname: Gu
authors.forename: Qiaoquan
authors.initials: Q
authors.lastname: Liu
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000235:genetics* / Q000378:metabolism; D000688:Amylose / Q000737:chemistry; D012275:Oryza / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D016372:RNA, Antisense / Q000235:genetics* / Q000378:metabolism; D013213:Starch / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D010940:Plant Proteins; D016372:RNA, Antisense; D013213:Starch; D000688:Amylose; D015061:1,4-alpha-Glucan Branching Enzyme