[Multilocus sequence typing of animal source Campylobacter jejuni in East China].
OBJECTIVE
To characterize the genes of Campylobacter jejuni isolates and to genotype the strains from chicken, cow, duck, pig and red-crowned Crane during 2006 to 2008 by multilocus sequence typing (MLST).


METHODS
To choose 7 pairs Campylobacter jejuni home-keeping gene, gltA, aspA, glnA, glyA, pgm, tkt and uncA as target gene, PCR was applied to detect 42 samples in East China between 2006-2008. Sequencing, analysis and comparation of the genes were done.


RESULTS
Typing the 42 isolates by MLST and finding 24 new sequence typing types.


CONCLUSIONS
MLST is a useful method to evuluate Campylobacter jejuni genes and gene evolution.
Xue
Feng
F
Plant and Food Inspection Center, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, China. feixu0905@yahoo.com.cn
Xu
Fei
F
Luan
Jun
J
Li
Zhenzhong
Z
Zhu
Changqing
C
Jiang
Yuan
Y
Lu
Chengping
C
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle; D002645:Chickens; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013552:Swine
D001426:Bacterial Proteins; D004269:DNA, Bacterial