Recombinant human cytochrome P450 monooxygenases for drug metabolite synthesis.
Cytochrome P450 monooxygenases (CYPs) are important enzymes in the metabolism of xenobiotics. Therefore, several approaches to clone and overexpress the human isoforms have been made. In addition to microsomes or S9 preparations, these recombinant human isoforms have found diverse application in drug development. We discuss and give examples of the use of bacterial whole cell systems with rec. human CYPs for the preparative scale synthesis of drug metabolites.
Schroer
Kirsten
K
Novartis Institutes for Biomedical Research, GDC/PSB, WSJ-508.2.02A, CH-4056 Basel, Switzerland.
Kittelmann
Matthias
M
Lütz
Stephan
S
D001419:Bacteria / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007218:Industrial Microbiology / Q000379:methods; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015262:Xenobiotics / Q000378:metabolism
D007527:Isoenzymes; D004364:Pharmaceutical Preparations; D011994:Recombinant Proteins; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases