title: Analysis of the association between lactotransferrin (LTF) gene polymorphism and dental caries.
abstract: OBJECTIVE
  The present study evaluated the association between lactotransferrin (LTF) gene polymorphism (exon 2, A/G, Lys/Arg) and dental caries.
  
  
  METHODS
  A convenience sample of 110 individuals, 12 years old, was divided into: group 1, 48 individuals without caries experience (DMFT=0), and group 2, 62 subjects with caries experience (DMFT>or=1). DNA was obtained from a mouthwash with 3% glucose solution, followed by a scrapping of the oral mucosa. After DNA purification, polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP) was performed to access the study polymorphism. The LTF A/G (Lys/Arg) polymorphism had been previously reported as located in exon 1.
  
  
  RESULTS
  Allele 1 of the study polymorphism was associated with low DMFT index and showed a protective effect against caries experience (OR=0.16, IC=0.03-0.76, p=0.01).
  
  
  CONCLUSIONS
  Lactotransferrin A/G (exon 2, Lys/Arg) polymorphism was associated with susceptibility to dental caries in 12-year-old students.
authors.affiliation: Center for Health and Biological Sciences, Pontifical Catholic University of Paraná, Curitiba, PR, Brazil.
authors.forename: Luiza Foltran
authors.initials: LF
authors.lastname: Azevedo
authors.forename: Giovana Daniela
authors.initials: GD
authors.lastname: Pecharki
authors.forename: João Armando
authors.initials: JA
authors.lastname: Brancher
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Cordeiro
authors.forename: Kamilla Gabriella dos Santos
authors.initials: KG
authors.lastname: Medeiros
authors.forename: Alessandra Armstrong
authors.initials: AA
authors.lastname: Antunes
authors.forename: Eduardo Silva
authors.initials: ES
authors.lastname: Arruda
authors.forename: Renata Iani
authors.initials: RI
authors.lastname: Werneck
authors.forename: Luciana Reis
authors.initials: LR
authors.lastname: de Azevedo
authors.forename: Rui Fernando
authors.initials: RF
authors.lastname: Mazur
authors.forename: Samuel Jorge
authors.initials: SJ
authors.lastname: Moysés
authors.forename: Simone Tetü
authors.initials: ST
authors.lastname: Moysés
authors.forename: Fábio Rueda
authors.initials: FR
authors.lastname: Faucz
authors.forename: Paula Cristina
authors.initials: PC
authors.lastname: Trevilatto
mesh_terms: D000483:Alleles; D019943:Amino Acid Substitution; D001120:Arginine; D001483:Base Sequence; D016022:Case-Control Studies; D002648:Child; D004245:DMF Index; D004252:DNA Mutational Analysis; D003731:Dental Caries / Q000235:genetics*; D003733:Dental Caries Susceptibility / Q000235:genetics*; D005787:Gene Frequency; D006801:Humans; D007781:Lactoferrin / Q000235:genetics*; D008239:Lysine; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D012463:Saliva / Q000378:metabolism
chemical_list: D001120:Arginine; D007781:Lactoferrin; D008239:Lysine