Production and extraction optimization of xylanase from Aspergillus niger DFR-5 through solid-state-fermentation.
The effects of solid substrates, initial moisture content, moistening medium, temperature and incubation time on xylanase production by Aspergillus niger DFR-5 was studied and the highest activity (2596 IU/g dry substrate (gds)) was achieved in medium that contained wheat bran (WB) and soybean cake (SBC) at a ratio of 70:30, was moistened to 70% with MSS-2 mineral salt solution, and incubated for 6 days at 40 degrees C. Water at 37 degrees C was suitable for efficient recovery of enzyme from moldy WB-SBC medium. The extraction parameters for xylanase were optimized with respect to minimum volume of extractant using a central composite rotatable design (CCRD). The maximum recovery of xylanase (4465+/-52 IU/gds) with 92.5% desirability was obtained employing water (10 ml/gds) as extractant at 200 rpm for 60 min. The result shows that an overall 5.4-fold increase in xylanase production was obtained in concentrated form by optimizing medium components and extraction conditions.
Pal
Ajay
A
Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Siddarthanagar, Mysore, India. ajaydrdo@rediffmail.com
Khanum
Farhath
F
D000383:Agriculture; D000704:Analysis of Variance; D001234:Aspergillus niger / Q000187:drug effects / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis* / Q000302:isolation & purification; D005285:Fermentation / Q000187:drug effects; D007221:Industry; D007753:Laboratories; D012044:Regression Analysis; D012997:Solvents / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014866:Waste Products / Q000032:analysis; D014867:Water / Q000737:chemistry
D012997:Solvents; D014866:Waste Products; D014867:Water; D043364:Endo-1,4-beta Xylanases