Evaluation of HLA-G5 plasmatic levels during pregnancy and relationship with the 14-bp polymorphism.
OBJECTIVE
Plasmatic HLA-G levels increase during pregnancy, but the contribution of each different isoform has not been elucidated yet.


METHODS
HLA-G5 was analyzed by ELISA in 19 controls, 79 women in the first 8 weeks of pregnancy and in nine women monthly until delivery. Genotyping for the 14-bp polymorphism was performed by PCR amplification of exon 8.


RESULTS
HLA-G5 was detected in plasma from 80% of pregnant women. The levels did not change during pregnancy, and there were no differences compared to control non-pregnant women. There was a high interindividual variation that was maintained throughout the pregnancy. The presence of +14-bp allele was associated with HLA-G5 positivity. Pregnant women who were heterozygotic to 14-bp polymorphism had significantly higher levels of HLA-G5 compared to -14 bp/-14-bp homozygotic.


CONCLUSIONS
Plasmatic HLA-G5 levels do not change during pregnancy and its concentration depends on 14-bp polymorphism.
Gonzalez
Alvaro
A
Department of Biochemistry, University Clinic of Navarra, Pamplona, Spain. agonzaleh@unav.es
Alegre
Estibaliz
E
Torres
Maria I
MI
Díaz-Lagares
Angel
A
Lorite
Pedro
P
Palomeque
Teresa
T
Arroyo
Ainhoa
A
D000328:Adult; D015153:Blotting, Western; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D014644:Genetic Variation; D006680:HLA Antigens / Q000097:blood* / Q000235:genetics; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000097:blood* / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011247:Pregnancy / Q000097:blood* / Q000235:genetics; D020033:Protein Isoforms; D018709:Statistics, Nonparametric
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D020033:Protein Isoforms; D004247:DNA