Polysialylated neuropilin-2 enhances human dendritic cell migration through the basic C-terminal region of CCL21.
Dendritic cell (DC) migration to secondary lymphoid organs is a critical step to properly exert its role in immunity and predominantly depends on the interaction of the chemokine receptor CCR7 with its ligands CCL21 and CCL19. Polysialic acid (PSA) has been recently reported to control CCL21-directed migration of mature DCs. Here, we first demonstrate that PSA present on human mature monocyte-derived dendritic cells did not enhance chemotactic responses to CCL19. We have also explored the molecular mechanisms underlying the selective enhancing effect of PSA on CCL21-driven chemotaxis of DCs. In this regard, we found out that prevention of DC polysialylation decreased CCL21 activation of JNK and Akt signaling pathways, both associated with CCR7-mediated chemotaxis. We also report that the enhanced PSA-mediated effect on DC migration towards CCL21 relied on the highly basic C-terminal region of this chemokine and depended on the PSA acceptor molecule neuropilin-2 (NRP2) and on the polysialyltransferase ST8SiaIV. Altogether, our data indicate that the CCR7/CCL21/NRP2/ST8SiaIV functional axis constitutes an important guidance clue for DC targeting to lymphoid organs.
Rey-Gallardo
Angela
A
Departamento de Microbiología Molecular y Biología de las Infecciones, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
Escribano
Cristina
C
Delgado-Martín
Cristina
C
Rodriguez-Fernández
José L
JL
Gerardy-Schahn
Rita
R
Rutishauser
Urs
U
Corbi
Angel L
AL
Vega
Miguel A
MA
D000595:Amino Acid Sequence; D024361:Amino Acids, Basic / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D019556:COS Cells; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D054421:Chemokine CCL21 / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D002522:Chlorocebus aethiops; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D039943:Neuropilin-2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D054730:Protein Interaction Domains and Motifs / Q000187:drug effects / Q000502:physiology; D011499:Protein Processing, Post-Translational / Q000502:physiology; D034741:RNA, Small Interfering / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D012794:Sialic Acids / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D024361:Amino Acids, Basic; C515478:CCL21 protein, human; D054421:Chemokine CCL21; D039943:Neuropilin-2; D034741:RNA, Small Interfering; D012794:Sialic Acids; C021319:polysialic acid