title: Dic (17;20) (p11;q11) preceded MLL gene amplification in a patient with de novo mixed-lineage leukemia.
abstract: We report a case of acute mixed-lineage leukemia, as seen in a 65 year-old female with MLL gene amplification and biallelic loss of wild type p53 gene. The diagnosis was based on the findings that her bone marrow (BM) blasts expressed cytoplasmic CD3 (cyCD3), B-lineage antigens and myeloid antigens accompanied by clonal rearrangements of IgH gene. The BM blasts consisted of small-sized peroxidase-negative blasts (97%) and large-sized peroxidase-positive blasts (3%). The BM blasts showed a complex "karyotype," including dic(17;20) (p11;q11), -5 and add (11q23). Add (11q23) abnormality was found in sideline karyotypes as well as the stemline abnormality of dic(17;20) (p11;q11). For the p53 gene, which is located at 17p13, fluorescence in situ hybridization analysis showed the loss of one of two p53 alleles. Furthermore, polymerase chain reaction-single-strand conformation polymorphism and following nucleotide sequencing showed that the p53 gene was mutated at codon 215, leading to an amino acid substitution from Ser to Arg. For the MLL gene, southern blot analysis showed that the MLL gene locus was amplified but not rearranged at its breakpoint cluster region, which is usually rearranged in balanced translocations with many partner genes. These findings suggest that MLL gene amplification may in this case be based on the genetic instability caused by the preceding biallelic loss of the wild type p53 gene.
authors.affiliation: First Department of Internal Medicine, Kansai Medical University. mkojima@dokkyomed.ac.jp
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Amakawa
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hiramoto
authors.forename: Seiji
authors.initials: S
authors.lastname: Kawano
authors.forename: Akira
authors.initials: A
authors.lastname: Hyo
authors.forename: Naoto
authors.initials: N
authors.lastname: Nakamichi
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Tajima
authors.forename: Tomoki
authors.initials: T
authors.lastname: Ito
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Mori
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kishimoto
authors.forename: Shirou
authors.initials: S
authors.lastname: Fukuhara
mesh_terms: D001853:Bone Marrow; D002880:Chromosomes, Human, Pair 11; D002886:Chromosomes, Human, Pair 17; D005260:Female; D005784:Gene Amplification; D016158:Genes, p53; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015456:Leukemia, Biphenotypic, Acute / Q000235:genetics*; D014178:Translocation, Genetic