Antimicrobial resistant bacteria among health care workers in intensive care units at Ain Shams University Hospitals.
Fifty HCWs in ICUs of Internal medicine, Chest, Neonatology and Burn were included in prospective cohort study. Collection of nasal, hand and rectal swabs, proper biochemical identification, culture media and antibiotic sensitivity tests were used to detect Methicillin-resistant Staphylococcus aureus (MRSA); vancomycin-resistant Enterococci (VRE) & extended spectrum beta-lactamase producing gram -ve bacilli (ESBLs). S. aureus was isolated from 34% of HCWs; 28% were nasal carriers, 4% were hand carriers and 2% had S. aureus at both sites. Nasal and hand carriage rates of MRSA were 20% & 4% respectively, with an overall rate of 22%. Gram -ve bacilli were isolated from 8% of HCWs hand swabs & showed Citrobacter koseri, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. Hand carriage rate of ESBLs was 2%. Hand contamination with gram -ve bacilli and S. aureus was in 14% of HCWs. VRE carriage rate was 9.5%. ESBLs carriage rate in rectal swabs was 21.43%. K. pneumoniae was the most common ESBLs producing isolate (33.3%), followed by E. coli (18.75%). In combined disc method, aztreonam was the most sensitive (90%) in detecting ESBLs. Burn ICU had highest % of MRSA & ESBLs carriage. Neonatal ICU showed highest % of VRE carriage. An insignificant association was between infection control training or antimicrobial intake and carriage of antimicrobial resistant bacteria.
Abdel Rahman
Amany Th
AT
Department of Microbiology and Immunology, Faculty of Medicine, Ain Shams University, Cairo 11566.
Hafez
Shereen F
SF
Abdelhakam
Sara M
SM
Ali-Eldin
Zainab A
ZA
Esmat
Ibrahim M E Abd El-Hamid
IM
Elsayed
Marwa S
MS
Aboul-Fotouh
Aisha
A
D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001424:Bacterial Infections / Q000453:epidemiology / Q000382:microbiology*; D002353:Carrier State; D015331:Cohort Studies; D024881:Drug Resistance, Bacterial; D004534:Egypt; D005260:Female; D006282:Health Personnel; D006801:Humans; D007362:Intensive Care Units; D008297:Male; D008875:Middle Aged; D009666:Nose / Q000382:microbiology; D055815:Young Adult
D000900:Anti-Bacterial Agents