title: Experimental verification of protective effect of hydrogen-rich water against cisplatin-induced nephrotoxicity in rats using dynamic contrast-enhanced CT.
abstract: Our aim was to assess the protective effect of hydrogen-rich water against cisplatin-induced nephrotoxicity in rats using dynamic contrast-enhanced CT (DCE-CT). DCE-CT studies were performed in 30 rats (8 weeks old) on days 0, 2, 4 and 7 using multidetector row CT. The rats were divided into three groups: a control group (n = 6) with free access to standard water and without cisplatin injection, a non-treatment group (n = 12) with free access to standard water and injected with cisplatin (3.6 mg kg(-1) body weight) intraperitoneally on day 0 and a treatment group (n = 12) with free access to hydrogen-rich water starting from 7 days before cisplatin injection. The contrast clearance per unit renal volume (K(1)) was estimated from the DCE-CT data using the Patlak model. The contrast clearance of the entire kidney (K) was obtained by multiplying K(1) by the renal volume. The serum creatinine level was also measured on day 7. The K(1) and K values normalised by those on day 0 in the treatment group were significantly greater than those in the non-treatment group on days 2, 4 and 7. There were no significant differences in the normalised K value between the treatment and control groups on days 2 and 7. The serum creatinine level in the treatment group was significantly lower than that in the non-treatment group and was not significantly different from that in the control group. This study demonstrated that hydrogen-rich water ameliorates renal dysfunction due to cisplatin-induced nephrotoxicity in rats.
authors.affiliation: Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kitamura
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Matsushita
authors.forename: H
authors.initials: H
authors.lastname: Fujinawa
authors.forename: K
authors.initials: K
authors.lastname: Murase
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D016022:Case-Control Studies; D002945:Cisplatin / Q000633:toxicity*; D003287:Contrast Media; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D006859:Hydrogen / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014057:Tomography, X-Ray Computed / Q000379:methods; D014867:Water
chemical_list: D000970:Antineoplastic Agents; D003287:Contrast Media; D014867:Water; D006859:Hydrogen; D003404:Creatinine; D002945:Cisplatin