Importance of TLR2 in the direct response of T lymphocytes to Schistosoma mansoni antigens.
The immunomodulatory effect of Schistosoma mansoni antigens has often been attributed to interaction with PRR expressed on APC. Our previous work has shown that S. mansoni-soluble egg antigen (SEA) can induce, together with a Th2 response, TGF-β-dependent Foxp3 expression in naïve CD4(+) T cells from NOD mice. We found that SEA can directly upregulate the expression of surface-bound TGF-β in purified CD4(+) T cells in the absence of accessory cell interactions. In this study, we show that the C-type lectin receptors DEC-205 and galectin-3 were involved in the direct interaction between S. mansoni antigens and CD4(+) T cells. SEA was able to enhance CD4(+) T-cell secretion of bioactive TGF-β in response to TLR2 ligand stimulation, in the absence of APC. We also show that TLR2 expressed on CD4(+) T cells was important for the Foxp3 expression induced by SEA.
Burton
Oliver T
OT
Department of Pathology, University of Cambridge, Cambridge, UK.
Gibbs
Sarah
S
Miller
Nigel
N
Jones
Frances M
FM
Wen
Li
L
Dunne
David W
DW
Cooke
Anne
A
Zaccone
Paola
P
D000818:Animals; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D000947:Antigens, Helminth / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D002478:Cells, Cultured; D005260:Female; D051858:Forkhead Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D037502:Galectin 3 / Q000276:immunology / Q000378:metabolism; D015801:Helminth Proteins / Q000276:immunology*; D054884:Host-Pathogen Interactions; D056747:Immunomodulation; D037181:Lectins, C-Type / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism; D012550:Schistosoma mansoni / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
D015703:Antigens, CD; D000947:Antigens, Helminth; C093569:DEC-205 receptor; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D037502:Galectin 3; D015801:Helminth Proteins; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; D016212:Transforming Growth Factor beta; C079442:gp50 protein, Schistosoma