title: [Protective effect of carnosine on the injury of rat vascular endothelial cells induced by hypoxia].
abstract: OBJECTIVE
  To investigate the effect of camosine on the injury of rat vascular endothelial cells(VECs) induced by hypoxia.
  
  
  METHODS
  The model of the injury of rat VECs induced by hypoxia was established. The effect of camosine on injury of VECs activity induced by hypoxia was determined by MTT assay. The levels of lactate dehydrogenase (LDH) activity in cell medium were measured with corresponding kit. The cell structure was observed under microscope after Coomassie brilliant blue R-250 staining.
  
  
  RESULTS
  After culturing VECs with camosine (10 to 20 mmol/L) for 6 hours, the decrease in VECs activity induced by 12 and 24 hour hypoxia was inhibited. The release of LDH was also inhibited, and the integrity of cell structure remained.
  
  
  CONCLUSIONS
  Camosine has the protective effect on hypoxia injured VECs.
authors.affiliation: Department of Biochemistry and Molecular Biology, Liaoning Medical University, Jinzhou 110021, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Bai
authors.forename: Ge
authors.initials: G
authors.lastname: Chi
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Li-na
authors.initials: LN
authors.lastname: Liu
authors.forename: Miao
authors.initials: M
authors.lastname: Fan
authors.forename: Hai-dong
authors.initials: HD
authors.lastname: Liu
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D002336:Carnosine / Q000494:pharmacology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000473:pathology*; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology*; D008297:Male; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016166:Free Radical Scavengers; D020011:Protective Agents; D002336:Carnosine