[Construction of cDNA library of protozoan ciliates Euplotes octocarinatus].
The protein biosynthesis in cells is an open process cooperatively regulated by many protein factors and enzymes, which form a complicated protein network and signal transduction pathway for mRNA metabolism and protein translation.


OBJECTIVE
To provide a platform for studying on the function of proteins involving in process of translation termination and the relationship among these proteins in ciliates.


METHODS
We constructed the cDNA library of protozoan ciliates Euplotes octocarinatus strictly following the procedure of BD Matchmaker Library Construction & Screening kit from Clontech.


RESULTS
We obtained a cDNA library of ciliates Euplotes, the titer of which was about 2.437 x 10(7) cfu/mL, which could suffice for functional gene screen. By using the class II polypeptide release factor (eRF3) as bait protein, we obtained some putative genes, including a partial cDNA putatively encoding RNA helicase.


CONCLUSIONS
This library will provide a convenient platform for identifying the functional genes in ciliates.
Chai
Baofeng
B
Key Laboratory of Chemical Biology and Molecular Engineering of The Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China. bfchai@sxu.edu.cn
Hao
Yanqin
Y
Li
Cui
C
Shen
Quan
Q
Liang
Aihua
A
D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics; D016812:Euplotes / Q000235:genetics*; D015723:Gene Library; D010454:Peptide Termination Factors / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics
D016054:DNA, Protozoan; D010454:Peptide Termination Factors; D015800:Protozoan Proteins; C087971:peptide-chain-release factor 3