Microwave-assisted acid extraction of selenium from medicinal plants followed by electrothermal atomic absorption spectrometric determination.
A simple and rapid microwave-assisted extraction (MAE) of selenium (Se) from medicinal plants was investigated using different concentrations of nitric acid and hydrochloric acid, sample mass, heating time, microwave energy, and plant particle size. The optimization strategy was carried out using multivariate methodologies. Electrothermal atomic absorption spectrometry was used to determine Se. The accuracy of the optimized procedure was evaluated by using certified reference materials with certified values for Se and a microwave-assisted acid digestion (MD) method for comparative purpose. No significant difference was observed (P > 0.05) when comparing the values obtained by the proposed MAE and MD methods (paired t-test). Under the optimum operating conditions, the LOD obtained from the standard calibration curve was 0.012 microg/L for Se. The average RSD of the MAE method varied between 4.05 and 7.53% (n = 6). The proposed method was successfully applied for the determination of Se in medicinal plants used as remedies for cancerous and infection diseases.
Kolachi
Nida Fatima
NF
University of Sindh, Center of Excellence in Analytical Chemistry, Jamshoro 76080, Pakistan.
Kazi
Tasneem Gul
TG
Baig
Jameel Ahmed
JA
Kandhro
Ghulam Abbas
GA
Khan
Sumaira
S
Afridi
Hassan Imran
HI
Kumar
Sham
S
Shah
Abdul Qadir
AQ
D000042:Absorption; D000465:Algorithms; D002138:Calibration; D002623:Chemistry Techniques, Analytical; D004563:Electrochemistry / Q000379:methods; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D008872:Microwaves; D015233:Models, Statistical; D010946:Plants, Medicinal / Q000378:metabolism*; D015203:Reproducibility of Results; D012643:Selenium / Q000032:analysis*; D013054:Spectrophotometry, Atomic / Q000379:methods*; D013499:Surface Properties
D012643:Selenium; D006851:Hydrochloric Acid