Comparative characterization of porcine mesenchymal stem cells derived from bone marrow extract and skin tissues.
Mesenchymal stem cells (MSCs) offer a great promise for regenerative medicine. Present study compared the characterization of porcine MSCs (pMSCs) derived from bone marrow extract with adult ear and fetal skin-derived cells on morphology, cell growth, alkaline phosphatase activity, proliferation ability, expression of cluster of differentiation (CD) markers (CD29, 45, and 90), cell cycle, protein and mRNA levels of Oct-4, Sox-2, and Nanog, and lineage differentiation ability. Skin-derived cells exhibited alkaline phosphatase activity and differentiation ability like pMSCs. pMSCs possessed a longer doubling time than skin-derived cells, and there was no difference in the ratio of G0/G1 phase between pMSCs and skin-derived cells. Except for CD29 and 90, all cells were found negative for CD45. Protein and mRNA expression of Oct-4, Sox-2 and Nanog were observed with similar intensity in all cells. Taken together, pMSCs and skin-derived cells revealed similar characteristics, and suggested the possible supportive role of skin-derived cells with MSCs for the regeneration of damaged tissues in cell-based therapies.
Ock
Sun-A
SA
College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea.
Jeon
Byeong-Gyun
BG
Rho
Gyu-Jin
GJ
D053687:Adult Stem Cells / Q000166:cytology / Q000502:physiology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005260:Female; D005333:Fetus / Q000166:cytology; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D011247:Pregnancy; D012867:Skin / Q000166:cytology*; D012879:Skin Physiological Phenomena; D013552:Swine; D014020:Tissue Extracts / Q000502:physiology
D014020:Tissue Extracts