title: The salivary gland transcriptome of the eastern tree hole mosquito, Ochlerotatus triseriatus.
abstract: Saliva of blood-sucking arthropods contains a complex mixture of peptides that affect their host's hemostasis, inflammation, and immunity. These activities can also modify the site of pathogen delivery and increase disease transmission. Saliva also induces hosts to mount an antisaliva immune response that can lead to skin allergies or even anaphylaxis. Accordingly, knowledge of the salivary repertoire, or sialome, of a mosquito is useful to provide a knowledge platform to mine for novel pharmacological activities, to develop novel vaccine targets for vector-borne diseases, and to develop epidemiological markers of vector exposure and candidate desensitization vaccines. The mosquito Ochlerotatus triseriatus is a vector of La Crosse virus and produces allergy in humans. In this work, a total of 1,575 clones randomly selected from an adult female O. triseriatus salivary gland cDNA library was sequenced and used to assemble a database that yielded 731 clusters of related sequences, 560 of which were singletons. Primer extension experiments were performed in selected clones to further extend sequence coverage, allowing for the identification of 159 protein sequences, 66 of which code for putative secreted proteins. Supplemental spreadsheets containing these data are available at http://exon.niaid.nih.gov/transcriptome/Ochlerotatus_triseriatus/S1/Ot-S1.xls and http://exon.niaid. nih.gov/transcriptome/Ochlerotatus_triseriatus/S2/Ot-S2.xls.
authors.affiliation: Section of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Calvo
authors.forename: Irma
authors.initials: I
authors.lastname: Sanchez-Vargas
authors.forename: Michalis
authors.initials: M
authors.lastname: Kotsyfakis
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Favreau
authors.forename: Kent D
authors.initials: KD
authors.lastname: Barbian
authors.forename: Van M
authors.initials: VM
authors.lastname: Pham
authors.forename: Kenneth E
authors.initials: KE
authors.lastname: Olson
authors.forename: José M C
authors.initials: JM
authors.lastname: Ribeiro
mesh_terms: D000595:Amino Acid Sequence; D000681:Amylases / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002688:Chitinases / Q000235:genetics; D019295:Computational Biology; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D044969:Ochlerotatus / Q000201:enzymology / Q000235:genetics*; D010447:Peptide Hydrolases / Q000235:genetics; D016133:Polymerase Chain Reaction; D012469:Salivary Glands / Q000502:physiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D000520:alpha-Glucosidases / Q000235:genetics
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D000681:Amylases; D002688:Chitinases; D000520:alpha-Glucosidases; D010447:Peptide Hydrolases