VEGF-induced corneal neovascularisation in a rabbit experimental model.
OBJECTIVE
Various conditions may cause vascularization of the normally avascular cornea. The aim of the present study was to create a reproducible experimental model that could enable the investigation of the phenomena leading to corneal vascularization. This involved creating a software to record the experimental data, enabling a subsequent digital analysis based on the growth models. The VEGF-induced pattern of neovascularization was also investigated.


METHODS
Twenty-seven rabbits divided in groups were used for the purposes of the present study. Some of them underwent intracorneal implants with or without vascular endothelial growth factor (VEGF) pellets, using an original microsurgical technique. Central and peripheral corneal burns were induced to other groups of animals in order to mimic the neovascularization process induced by inflammation. Finally, Dexamethasone (Maxidex) was given intraocularly, on days 1 and 3 after the onset of neovascularisation, in rabbit groups with both corneal burns and VEGF-implants. Video recording and data analysis of the corneal vascularization were made with an advanced biomicroscope, a computerized imaging system and a special software. A histochemical study of the animals' eyes was also carried out.


CONCLUSIONS
The recorded data showed the simplicity and reproducibility of the present experimental model. The results showed the importance of VEGF as an initiator and promoter of corneal vascularization through a non-inflammatory mechanism, quite different from the inflammation illustrated by the corneal burn. At the same time, Dexamethasone therapy proved its effectiveness in corneal angiogenesis induced by thermal burn, but not by VEGF-implant.
Coman
L
L
Department of Physiology, Faculty of Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania. laurentiucmn@yahoo.com
Coman
Oana Andreia
OA
Păunescu
H
H
Drăghia
Fl
F
Fulga
I
I
D000818:Animals; D016510:Corneal Neovascularization / Q000188:drug therapy*; D005126:Eye Burns / Q000503:physiopathology; D005260:Female; D008297:Male; D023421:Models, Animal; D011817:Rabbits; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
D042461:Vascular Endothelial Growth Factor A