Phenanthrenes from Dendrobium nobile and their inhibition of the LPS-induced production of nitric oxide in macrophage RAW 264.7 cells.
Bioactivity-guided isolation of the methanol extract of the stems of Dendrobium nobile yielded a new phenanthrene together with nine known phenanthrenes and three known bibenzyls. Their structures were elucidated by analysis of the spectroscopic data including 2D-NMR. All of the isolates were evaluated for their potential to inhibit the LPS-induced production of nitric oxide in murine macrophage RAW 264.7 cells. Compounds 1-4, 7-13 inhibited nitric oxide production with the IC(50) values ranging from 9.6 microM to 35.7 microM.
Hwang
Ji Sang
JS
College of Pharmacy, Chungbuk National University, Cheongju, Republic of Korea.
Lee
Seon A
SA
Hong
Seong Su
SS
Han
Xiang Hua
XH
Lee
Chul
C
Kang
Shin Jung
SJ
Lee
Dongho
D
Kim
Youngsoo
Y
Hong
Jin Tae
JT
Lee
Mi Kyeong
MK
Hwang
Bang Yeon
BY
D000818:Animals; D002460:Cell Line; D031667:Dendrobium / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010616:Phenanthrenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts; D013329:Structure-Activity Relationship
D008070:Lipopolysaccharides; D010616:Phenanthrenes; D010936:Plant Extracts; D009569:Nitric Oxide