[Carbon monoxide inhalation protects against limb ischemia/reperfusion injury].
OBJECTIVE
To study the protective effect of carbon monoxide(CO) inhalation in the limb ischemia/reperfusion (I/R) injury of rats.


METHODS
Forty-four Sprague-Dawley rats were randomly divided into three groups: S, I/R and RC groups. I/R injury model was made by the occlusion of the femoral artery for four hours and the reperfusion for forty-eight hours. RC group was exposed to medical air mixed CO (the volume fraction of CO: 0.05%) during limb reperfusion in rats. The other two groups were exposed to the routine air. Gross morphology of the ischemic limb, wet-to-dry weight ratio (W/D), and skeletal muscle histopathology were observed. The apoptosis index and expression levels of Bax and Bcl-2 in the muscle were assessed with Flow Cytometry. The activities of serum lactate dehydrogenase (LDH) and creatine kinase (CK) were tested by Automatic Biochemical Analyzer.


RESULTS
Compared to I/R group, W/D, serum LDH and CK activities, the apoptosis index and Bax expression level in the muscle were all significantly decreased, the Bcl-2 expression level was significantly increased, gross morphology of the ischemic limb and muscle histopathology were obviously improved in RC group.


CONCLUSIONS
Inhaling exogenous CO can attenuate limb I/R injury.
Li
Jing
J
Department of Anatomy, Hebei Medical University, Shijiazhuang 050017, China.
Zhu
Zhen-long
ZL
Qi
Wan-zheng
WZ
Shi
Zhong-li
ZL
D000280:Administration, Inhalation; D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology*; D003402:Creatine Kinase / Q000378:metabolism; D005121:Extremities / Q000098:blood supply*; D005263:Femoral Artery; D007511:Ischemia / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D002248:Carbon Monoxide; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase