title: Narrow-band imaging endoscopy to assess mucosal angiogenesis in inflammatory bowel disease: a pilot study.
abstract: OBJECTIVE
  To investigate whether narrow band imaging (NBI) is a useful tool for the in vivo detection of angiogenesis in inflammatory bowel disease (IBD) patients.
  
  
  METHODS
  Conventional and NBI colonoscopy was performed in 14 patients with colonic inflammation (8 ulcerative colitis and 6 Crohn's disease). Biopsy samples were taken and CD31 expression was assayed immunohistochemically; microvascular density was assessed by vessel count.
  
  
  RESULTS
  In areas that were endoscopically normal but positive on NBI, there was a significant (P < 0.05) increase in angiogenesis (12 +/- 1 vessels/field vs 18 +/- 2 vessels/field) compared with areas negative on NBI. In addition, in areas that were inflamed on white light endoscopy and positive on NBI, there was a significant (P < 0.01) increase in vessel density (24 +/- 7 vessels/field) compared with NBI-negative areas.
  
  
  CONCLUSIONS
  NBI may allow in vivo imaging of intestinal angiogenesis in IBD patients.
authors.affiliation: Division of Gastroenterology, IRCCS Istituto Clinico Humanitas, Viale Manzoni, Rozzano, Milan 20089, Italy. sdanese@hotmail.com
authors.forename: Silvio
authors.initials: S
authors.lastname: Danese
authors.forename: Gionata
authors.initials: G
authors.lastname: Fiorino
authors.forename: Erika
authors.initials: E
authors.lastname: Angelucci
authors.forename: Stefania
authors.initials: S
authors.lastname: Vetrano
authors.forename: Nico
authors.initials: N
authors.lastname: Pagano
authors.forename: Giacomo
authors.initials: G
authors.lastname: Rando
authors.forename: Antonino
authors.initials: A
authors.lastname: Spinelli
authors.forename: Alberto
authors.initials: A
authors.lastname: Malesci
authors.forename: Alessandro
authors.initials: A
authors.lastname: Repici
mesh_terms: D001706:Biopsy; D003093:Colitis, Ulcerative / Q000276:immunology / Q000473:pathology*; D003106:Colon / Q000098:blood supply*; D003113:Colonoscopy / Q000379:methods*; D003424:Crohn Disease / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000098:blood supply*; D055806:Microvessels / Q000276:immunology / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000276:immunology / Q000473:pathology*; D010865:Pilot Projects; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D011237:Predictive Value of Tests; D012720:Severity of Illness Index
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1