Hyperbaric oxygen preconditioning ameliorates anxiety-like behavior and cognitive impairments via upregulation of thioredoxin reductases in stressed rats.
The present study examined the protective effect of hyperbaric oxygen preconditioning (HBO-PC) and the role of thioredoxin reductase (TrxR) in a post-traumatic stress disorder (PTSD)-induced rat model by using single prolonged stress (SPS). Rats were randomly divided into Sham, HBO, SPS and HBO+SPS groups. HBO-PC was conducted by exposing rats to 100% oxygen at 2.5atm absolute for 1h each day for 5 consecutive days. SPS was performed 24h after the last HBO-PC conditioning event. At 1h, 6h, 12h, 24h and 72h after SPS, TrxR mRNA expression was analyzed in the hippocampus; Nissl and TUNEL staining were performed at 72h after SPS. The results indicated that HBO-PC was able to significantly preserve viable neurons in the CA1 subfield of hippocampus following SPS exposure, as evidenced by reduced amounts of CA1 neuronal apoptosis. Furthermore, HBO-PC upregulate the expression of TrxR-1 and TrxR-2 mRNA in the hippocampus at 6h and 12h after SPS exposure and ameliorated anxiety-like behavior and cognitive impairments normally induced by SPS. Taken together, these findings suggest that HBO-PC is beneficial for the improvement of anxiety-like behavior and cognitive impairments induced by SPS exposure, and this effect might be associated with inhibition of neuronal apoptosis via upregulation of TrxR in stressed rats.
Peng
Ye
Y
Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Feng
Shu-Fang
SF
Wang
Qiang
Q
Wang
Hua-Ning
HN
Hou
Wu-Gang
WG
Xiong
Lize
L
Luo
Zhuo-Jing
ZJ
Tan
Qing-Rong
QR
D000704:Analysis of Variance; D000818:Animals; D001007:Anxiety / Q000378:metabolism / Q000628:therapy*; D017209:Apoptosis; D002452:Cell Count; D003072:Cognition Disorders / Q000378:metabolism / Q000628:therapy*; D006624:Hippocampus / Q000378:metabolism*; D006931:Hyperbaric Oxygenation; D020287:In Situ Nick-End Labeling; D008297:Male; D009043:Motor Activity; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological; D013315:Stress, Psychological; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism*; D015854:Up-Regulation
D012333:RNA, Messenger; D013880:Thioredoxin-Disulfide Reductase