Protective effect of Phyllanthus fraternus against bromobenzene induced mitochondrial dysfunction in rat liver mitochondria.
The objective of the current study was to investigate the protective effect of an aqueous extract of Phyllanthus fraternus (AEPF) against bromobenzene induced mitochondrial dysfunction in rat liver mitochondria. Administration of bromobenzene (10 mmol/kg body wt.) significantly decreased the rate of respiration (with glutamate+malate or succinate as substrates), abolished respiratory control ratio (RCR) and P/O ratios completely. There was a significant increase in the levels of lipid peroxides and protein carbonyls and a significant decrease in the total sulphydryl groups. The activities of antioxidant enzymes like catalase, glutathione peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase (SOD) were decreased. The levels of antioxidants like reduced and oxidized glutathione were significantly decreased compared to control. Administration of rats with an AEPF (100mg/kg body wt.) prior to bromobenzene administration showed several beneficial effects like: (i) complete protection on mitochondrial respiration, RCR and P/O ratios (ii) lipid peroxides and protein carbonyl levels were significantly lowered (iii) increased the levels of sulphydryl groups and the activity of antioxidant enzymes and (iv) significant increase in the levels of reduced and oxidized glutathione. Vitamin E was used as positive control and bromobenzene induced mitochondrial dysfunction was protected better with AEPF compared to vitamin E.
Gopi
Sriram
S
Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India.
Setty
Oruganti H
OH
D000818:Animals; D001969:Bromobenzenes / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D002374:Catalase / Q000737:chemistry; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000473:pathology; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000473:pathology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D029803:Phyllanthus / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology*; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D001969:Bromobenzenes; D010936:Plant Extracts; D020011:Protective Agents; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; C032036:bromobenzene; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase