[The impact of diabetes mellitus and insulin interference on cortical SCF/KIT of mice].
OBJECTIVE
To investigate the expression of cortical SCF/KIT system at different blood glucose level in mice.


METHODS
27 male C57 mice were randomly divided into control group, diabetes group, and diabetes plus insulin group. The diabetic mice were induced by streptozotocin. Western-blot and double-immunofluorescence histochemistry were used to detect the expression of SCF and KIT.


RESULTS
Both methods indicate that the level of S-SCF and M-SCF were decreased significantly in the diabetes group, and this trend can be reversed effectively when the insulin was utilized.


CONCLUSIONS
The decline of SCF might be one of underlying mechanisms of diabetic encephalopathy.
Yun
Luo
L
Department of Neurology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China.
Feng-nan
Niu
N
Wen-jing
Zhu
Z
Yun
Xu
X
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D015536:Down-Regulation / Q000502:physiology; D007328:Insulin / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D019089:Stem Cell Factor / Q000378:metabolism*
D007328:Insulin; D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit