title: Combined omics analysis identifies transmembrane 4 L6 family member 1 as a surface protein marker specific to human mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) are promising for cell therapy and regenerative medicine; but their lack of specific markers renders the cell culture at potential contamination risk with other cell types, in particular, fibroblasts. In this study, we mapped 2 differential transcriptome data of MSCs compared, one to mononuclear cells and the other to fibroblasts, onto the membrane proteome data, the analysis of which led to an identification of transmembrane 4 L6 family member 1 (TM4SF1) as a surface protein marker candidate that could discriminate MSCs simultaneously from blood cells and fibroblasts. Our analyses confirmed that TM4SF1 was abundantly expressed on MSCs but neither on other blood/tissue cells nor on fibroblasts. TM4SF1 immunoselection from bone marrow and adipose tissues yielded homogeneous cell populations that were highly similar to MSCs, in terms of morphology, immunophenotype, and differentiation potential. These findings indicate that TM4SF1 can serve as a surface protein marker which singly identifies MSCs from diverse cell sources, in particular, fibroblast-rich connective tissues.
authors.affiliation: Biotherapeutic Division, Aprogen, Inc., Daejon, Korea.
authors.forename: Sohyun
authors.initials: S
authors.lastname: Bae
authors.forename: So Hee
authors.initials: SH
authors.lastname: Shim
authors.forename: Chae Woon
authors.initials: CW
authors.lastname: Park
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Son
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Son
authors.forename: Choonju
authors.initials: C
authors.lastname: Jeon
authors.forename: Hoeon
authors.initials: H
authors.lastname: Kim
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000737:chemistry / Q000378:metabolism; D000954:Antigens, Surface / Q000737:chemistry* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009000:Monocytes / Q000737:chemistry / Q000378:metabolism; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000378:metabolism; D020543:Proteome / Q000737:chemistry*
chemical_list: D000943:Antigens, Differentiation; D000954:Antigens, Surface; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D020543:Proteome; C074075:TM4SF1 protein, human