UV-induced grafting processes with in situ formed photomask for micropatterning of two-component biomolecules.
We report a photolithographic process for micropatterning of two-component biomolecules on a transparent organic film via lateral functional polymer brushes of poly(sodium acrylate) (P(AA)) and poly(glycidyl methacrylate) (P(GMA)). The pattern of binary polymer brushes were prepared via consecutive UV-initiated grafting processes, under the assistance of the in situ formed poly (4,4'-bi[N-(4-vinylbenzyl) pyridinium]) (P(BVV)) photomask. The epoxy groups of the P(GMA) microdomains can be aminated for covalently coupling biotin, while the P(AA) microdomains were used for immobilizing immunoglobulin (IgG). The resulting biotin- and IgG-coupled microdomains interact specifically with their corresponding target proteins, avidin and anti-IgG, respectively.
Tang
S C
SC
State Key Laboratory of Chemical Resource Engineering, Beijing, 100029, China.
Xie
J Y
JY
Huang
Z H
ZH
Xu
F J
FJ
Yang
Wantai
W
D000888:Antibodies, Anti-Idiotypic; D001360:Avidin; D001710:Biotin / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D014466:Ultraviolet Rays
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D011108:Polymers; D001360:Avidin; D001710:Biotin