title: Effects of nutritional level on pork quality and gene expression of micro-calpain and calpastatin in muscle of finishing pigs.
abstract: The study was designed to investigate the effects of nutritional level (control diet (CD), 14.19% crude protein, 13.81MJ of DE/kg; low nutritional level diet (LND), 11.08% crude protein, 12.55MJ of DE/kg) on pork quality and gene expression of mu-calpain and calpastatin in muscle of finishing pigs. The LND treatment increased drip loss (P<0.05), had a trend to increase intramuscular fat (IMF) content (P=0.09), decreased Warner-Bratzler shear force (WBSF) of pork (P<0.05), improved mRNA level of mu-calpain (P<0.05) in skeletal muscle, but had no effect on gene expression of calpastatin, compared with the CD treatment. These data suggest that a moderately reduced energy and protein diet increased pork tenderness and intramuscular fat. The increase in tenderness by LND treatment may be partly due to increased gene expression of mu-calpain in muscle.
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
authors.forename: Renyong
authors.initials: R
authors.lastname: Tang
authors.forename: Bing
authors.initials: B
authors.lastname: Yu
authors.forename: Keying
authors.initials: K
authors.lastname: Zhang
authors.forename: Xiulan
authors.initials: X
authors.lastname: Guo
authors.forename: Gang
authors.initials: G
authors.lastname: Tian
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Huang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.forename: Daiwen
authors.initials: D
authors.lastname: Chen
mesh_terms: D000821:Animal Feed / Q000592:standards; D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002154:Calpain / Q000235:genetics / Q000378:metabolism*; D004032:Diet / Q000592:standards* / Q000662:veterinary; D002149:Energy Intake; D005223:Fats / Q000032:analysis; D005524:Food Technology; D015870:Gene Expression; D008460:Meat / Q000592:standards*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009753:Nutritive Value; D012333:RNA, Messenger / Q000378:metabolism; D013314:Stress, Mechanical; D013552:Swine
chemical_list: D002135:Calcium-Binding Proteins; D005223:Fats; D009124:Muscle Proteins; D012333:RNA, Messenger; C033668:calpastatin; D002154:Calpain