Efficient mRNA detection from human archival paraffin-embedded tissue: an update.
We recently published an in situ hybridization protocol for archival tissue using a commercial hybridization buffer. This buffer is no more available. Therefore, we have developed an improved protocol with a defined hybridization buffer.
Illig
Romana
R
Department of Anatomy, Division of Clinical and Functional Anatomy, Innsbruck Medical University, 6020 Innsbruck, Austria.
Fritsch
Helga
H
Schwarzer
Christoph
C
D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D007202:Indicators and Reagents; D016612:Paraffin Embedding / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis*
D007202:Indicators and Reagents; D012333:RNA, Messenger