Anterior development in the parthenogenetic and viviparous form of the pea aphid, Acyrthosiphon pisum: hunchback and orthodenticle expression.
In the dipteran Drosophila, the genes bicoid and hunchback work synergistically to pattern the anterior blastoderm during embryogenesis. bicoid, however, appears to be an innovation of the higher Diptera. Hence, in some non-dipteran insects, anterior specification instead relies on a synergistic interaction between maternally transcribed hunchback and orthodenticle. Here we describe how orthologues of hunchback and orthodenticle are expressed during oogenesis and embryogenesis in the parthenogenetic and viviparous form of the pea aphid, Acyrthosiphon pisum. A. pisum hunchback (Aphb) mRNA is localized to the anterior pole in developing oocytes and early embryos prior to blastoderm formation - a pattern strongly reminiscent of bicoid localization in Drosophila. A. pisum orthodenticle (Apotd), on the other hand, is not expressed prior to gastrulation, suggesting that it is the asymmetric localization of Aphb, rather than synergy between Aphb and Apotd, that regulates anterior specification in asexual pea aphids.
Huang
T-Y
TY
Department of Entomology/Institute of Biotechnology, National Taiwan University, Taipei, Taiwan.
Cook
C E
CE
Davis
G K
GK
Shigenobu
S
S
Chen
R P-Y
RP
Chang
C-C
CC
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000196:embryology* / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D001483:Base Sequence; D019521:Body Patterning / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D018398:Homeodomain Proteins / Q000235:genetics; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009866:Oogenesis / Q000235:genetics; D010312:Parthenogenesis / Q000235:genetics; D018532:Pisum sativum / Q000469:parasitology; D012313:RNA / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics; D052286:Viviparity, Nonmammalian / Q000235:genetics
D017931:DNA Primers; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D019476:Insect Proteins; D014157:Transcription Factors; C061245:hb protein, Drosophila; C093053:oc protein, Drosophila; D012313:RNA