Evaluation of the cytotoxicity of cell free dermal substitutes using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method.
BACKGROUND
The cytotoxicity of dermal substitutes may be increased by the very processes used to deplete the cells. The present research aimed to investigate the method for monitoring the cytotoxicity of cell-free dermal substitutes using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method.


METHODS
The cytotoxicity of four dermal substitutes was evaluated using the MTT method according to the standards set by the Chinese State Food and Drug Administration (SFDA). Swine acellular dermal matrix (SADM) and goat acellular dermal matrix (GADM) were produced using a repeated freeze-thaw method. Human dermal matrix glutaraldehyde composite (HADM-G) and SADM cross-linked with glutaraldehyde (SADM-G) were produced using conventional methods.


RESULTS
The cytotoxicity of all dermal substitutes ranged from Grade 0 to Grade 1, meeting the standards of the Chinese FDA. The OD(490) of both SADM and GADM was higher than that of either HADM-G or SADM-G (P < 0.05).


CONCLUSIONS
Dermal substitutes produced by the freeze-thaw method are less cytotoxic than those produced using conventional methods.
Ning
Fang-gang
FG
Burns Department, Beijing Jishuitan Hospital, China.
Zhang
Guo-an
GA
D002460:Cell Line; D006801:Humans; D017768:Skin, Artificial / Q000009:adverse effects*; D013778:Tetrazolium Salts / Q000737:chemistry*; D018675:Toxicity Tests / Q000379:methods*
D013778:Tetrazolium Salts