Enhanced diagnosis of pandemic (H1N1) 2009 influenza infection using molecular and serological testing in intensive care unit patients with suspected influenza.
During the 2009 outbreak of pandemic (H1N1) 2009 influenza (pH1N1) in Australia, acute and convalescent serum specimens were collected from 33 patients with severe respiratory disease admitted to intensive care units. Using hemagglutination inhibition of pH1N1, 29 paired serum samples showed significant increases in specific antibody titers. Of these 29 patients, 18 had pH1N1 RNA detected by routine nucleic acid testing. These results indicate that up to one-third of pH1N1 cases may not have laboratory confirmation of infection unless serological testing is included for suspected cases.
Iwasenko
Jenna M
JM
Department of Microbiology, South Eastern Area Laboratory Services, Prince of Wales Hospital, Randwick, New South Wales, Australia.
Cretikos
Michelle
M
Paterson
David L
DL
Gibb
Robert
R
Webb
Steven A
SA
Smith
David W
DW
Blyth
Christopher C
CC
Dwyer
Dominic E
DE
Shi
Jane Q
JQ
Robertson
Peter
P
Rawlinson
William D
WD
D000328:Adult; D000914:Antibodies, Viral; D001315:Australia / Q000453:epidemiology; D004196:Disease Outbreaks; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D007362:Intensive Care Units; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis; D012367:RNA, Viral / Q000097:blood
D000914:Antibodies, Viral; D009696:Nucleic Acids; D012367:RNA, Viral