Structural identification of mouse urinary metabolites of pterostilbene using liquid chromatography/tandem mass spectrometry.
Pterostilbene, the dimethoxy derivative of resveratrol, has drawn much attention recently due to its potential beneficial health effects. The metabolic fate of pterostilbene, however, is not well understood. In the present study, we identified nine novel mouse urinary pterostilbene metabolites, pterostilbene glucuronide, pterostilbene sulfate, mono-demethylated pterostilbene glucuronide, mono-demethylated pterostilbene sulfate, mono-hydroxylated pterostilbene, mono-hydroxylated pterostilbene glucuronide, mono-hydroxylated pterostilbene sulfate, and mono-hydroxylated pterostilbene glucuronide sulfate, using liquid chromatography/atmospheric pressure chemical ionization and electrospray ionization tandem mass spectrometry. The structures of these metabolites were confirmed by analyzing the MS(n) (n = 1-3) spectra. To our knowledge, this is the first report of the identification of urinary metabolites of pterostilbene in mice.
Shao
Xi
X
Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA.
Chen
Xiaoxin
X
Badmaev
Vladimir
V
Ho
Chi-Tang
CT
Sang
Shengmin
S
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D013267:Stilbenes / Q000378:metabolism* / Q000652:urine*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D013267:Stilbenes; C107773:pterostilbene