title: Differential apoptosis-like cell death in amastigote and trypomastigote forms from Trypanosoma cruzi-infected heart cells in vitro.
abstract: Apoptosis, type-I of programmed cell death (PCD-I), is not restricted to multicellular organisms since many apoptotic features have been described in different trypanosomatids, including Trypanosoma cruzi. Our present aim was to monitor, by different morphological markers, the occurrence of apoptosis-like death in amastigotes and trypomastigotes of T.cruzi (Y strain) during the infection of heart culture cells. We documented the differential occurrence of PCD-I in amastigotes and trypomastigotes, with distinct death rates noticed between these two parasite-distinct forms. Fluorescence microscopy and flow cytometry analysis using different hall markers of apoptosis (phosphatidylserine exposure, collapse of mitochondrial membrane potential and DNA fragmentation) showed that amastigotes present higher levels of apoptosis-like cell death as compared to trypomastigotes. It is possible that the higher levels of PCD-I in these highly multiplicative forms may contribute to the control of the parasite burden within the host cells. On the other hand, the apoptosis-like occurrence in the infective but non-proliferative stage of the parasite (trypomastigotes) may play a role in parasite evasion mechanisms as suggested for other parasites.
authors.affiliation: Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brasil. emello@ioc.fiocruz.br
authors.forename: E M
authors.initials: EM
authors.lastname: De Souza
authors.forename: A S G
authors.initials: AS
authors.lastname: Nefertiti
authors.forename: C
authors.initials: C
authors.lastname: Bailly
authors.forename: A
authors.initials: A
authors.lastname: Lansiaux
authors.forename: M N C
authors.initials: MN
authors.lastname: Soeiro
mesh_terms: D000818:Animals; D017209:Apoptosis; D014355:Chagas Disease / Q000469:parasitology* / Q000473:pathology*; D005434:Flow Cytometry; D006321:Heart / Q000469:parasitology*; D008018:Life Cycle Stages; D053078:Membrane Potential, Mitochondrial; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009206:Myocardium / Q000473:pathology; D010718:Phosphatidylserines / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000254:growth & development*
chemical_list: D010718:Phosphatidylserines