Functional comparison of catalase genes in the elimination of photorespiratory H2O2 using promoter- and 3'-untranslated region exchange experiments in the Arabidopsis cat2 photorespiratory mutant.
Photorespiration-associated production of H(2) O(2) accounts for the majority of total H(2) O(2) in leaves of C(3) plants and is mainly eliminated by catalases. In Arabidopsis, lack of CAT2, but not CAT1 or CAT3, results in growth suppression and a marked accumulation of H(2) O(2) in leaves. To evaluate the contribution of individual catalase genes and their promoters to catalase function, we investigated the growth suppression and H(2) O(2) accumulation phenotypes of Arabidopsis derivatives expressing catalase genes from heterologous CAT promoters in a cat2 mutant background. The expression of CAT2 from the CAT2 promoter restored the wild-type phenotype in a cat2-1 mutant, while CAT1 and CAT3 promoter-driven expression of CAT2 did not. Ectopic expression of CAT3 from the CAT2 promoter also restored the normal phenotype, unlike that of CAT1 which required replacement of the CAT1 3'-untranslated region (UTR) with that of CAT2. These results demonstrated that the photorespiratory role of CAT2 is determined mainly by the regulation of its promoter activity. The 3'-UTR of CAT2 was vital for controlling CAT2 protein levels under photorespiratory conditions. Identification of component of heterotetramers catalase isoforms suggested that there is some functional redundancy between CAT2 and CAT1 and CAT3.
Hu
Ye-Qin
YQ
Key Lab of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Liu
Sheng
S
Yuan
Hong-Mei
HM
Li
Jing
J
Yan
Da-Wei
DW
Zhang
Jian-Feng
JF
Lu
Ying-Tang
YT
D020413:3' Untranslated Regions; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D018744:DNA, Plant; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D016254:Mutagenesis, Insertional; D018384:Oxidative Stress; D010641:Phenotype; D018515:Plant Leaves; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms; D012323:RNA Processing, Post-Transcriptional; D018749:RNA, Plant
D020413:3' Untranslated Regions; D029681:Arabidopsis Proteins; D018744:DNA, Plant; D020033:Protein Isoforms; D018749:RNA, Plant; D006861:Hydrogen Peroxide; D002374:Catalase