title: Intracellular magnesium and tolerance against hypoxia in immature rat heart.
abstract: BACKGROUND
  Neonatal hearts show greater resistance to hypoxia than adult hearts do, but the mechanism is unclear. In immature rat hearts, we examined the association between high tolerance for hypoxia and the kinetics of magnesium (Mg), which has myocardial protective effects in adult hearts.
  
  
  METHODS
  The hearts of 1-, 2-, 3-, and 10-week-old rats were Langendorff perfused and subjected to 45 min hypoxia and 30 min reoxygenation. The myocardial Mg content was measured by atomic absorption spectrophotometry.
  
  
  RESULTS
  In 1-week-old rat hearts, the tension-rate product recovered more immediately with reoxygenation than in the other groups, and aspartate aminotransferase release did not increase throughout the experiment. In 10-week-old rat hearts, total tissue Mg content decreased significantly with reoxygenation (607.0 ± 22.9 µg/g dry weight) from its control level (793.2 ± 16.7 µg/g dry weight). However, in neonates, the total tissue Mg did not decrease with reoxygenation.
  
  
  CONCLUSIONS
  The high resistance against hypoxia-reoxygenation injury in neonatal rat hearts is caused, at least in part, by a high ability to maintain myocardial Mg.
authors.affiliation: Department of Pediatrics, Juntendo University School of Medicine, Hongo, Tokyo, Japan. tomo-st@jb4.so-net.ne.jp
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Sato
authors.forename: Makino
authors.initials: M
authors.lastname: Watanabe
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kishiro
authors.forename: Satoru
authors.initials: S
authors.lastname: Nagata
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Shimizu
authors.forename: Takao
authors.initials: T
authors.lastname: Okada
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001219:Aspartate Aminotransferases / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006339:Heart Rate / Q000502:physiology; D000860:Hypoxia / Q000503:physiopathology*; D008274:Magnesium / Q000378:metabolism*; D008297:Male; D009200:Myocardial Contraction / Q000502:physiology*; D017202:Myocardial Ischemia / Q000503:physiopathology*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control; D010100:Oxygen / Q000494:pharmacology; D010101:Oxygen Consumption / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013053:Spectrophotometry
chemical_list: D001219:Aspartate Aminotransferases; D008274:Magnesium; D010100:Oxygen