[Changes of proteomics in the injured lung of adult rats subjected to acrolein inhalation].
OBJECTIVE
To investigate the proteomics change in injured lung tissues of adult rats subjected to acrolein inhalation for 6 weeks.


METHODS
Two-dimensional electrophoresis was used to define the change of proteomic expresses and mass spectrometry was applied to identify the amino acid sequence in differential expressional proteins in lung tissues between acrolein inhalation group and saline control group.


RESULTS
A total of 545 protein spots were found in the lung tissues tested. Of the 545 proteins, 7 differentially expressed proteins were detected in acrolein inhalation group as compared with saline control group. Among them, tyrosine 3/tryptophan 5-monooxygenase activation protein, phosphatidylinositol transfer protein, poly(A)-binding protein, presented a marked upregulation, while 2601 and 2603 vimentin, phosphatidylinositol transfer protein, and chloride intracellular channel 3 showed a marked downregulation.


CONCLUSIONS
This study provides the crucial evidence that differential expressional proteins may underline the machenism of lung injury after acrolein inhalation in adult rats.
Dan
Qi-qin
QQ
Department of Respiration, the First People' s Hospital of Yunnan Province, Kunming 65000, China.
Li
Yun
Y
Zhang
Li
L
Zhao
Shan
S
Wang
Sheng-lan
SL
Yuan
Bin
B
Zhang
Yun-hui
YH
D000171:Acrolein / Q000008:administration & dosage / Q000633:toxicity*; D000280:Administration, Inhalation; D000818:Animals; D005260:Female; D008168:Lung / Q000378:metabolism / Q000473:pathology; D055370:Lung Injury / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D049294:Phospholipid Transfer Proteins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D049294:Phospholipid Transfer Proteins; D020543:Proteome; D000171:Acrolein; D014446:Tyrosine 3-Monooxygenase