title: Molecular and virological analyses of dengue virus responsible for dengue outbreak in East Timor in 2005.
abstract: A severe dengue outbreak occurred in East Timor in 2005. The dengue virus genome was detected by TaqMan RT-PCR in 40 serum samples, as follows: dengue virus type-3 (DENV-3) in 37 samples, DENV-2 in 2 samples, and DENV-1 in one sample. One DENV-1 genome, one DENV-2 genome, and 5 DENV-3 genomes were sequenced, and these specimens were aligned with the previously determined envelope (E) gene sequences. The DENV-1 strain belonged to genotype IV and was close to those previously isolated in Indonesia and Australia. The DENV-2 strain belonged to genotype I and was close to those previously isolated in Indonesia, Australia, the Far East, and India in 1993-2001. The DENV-3 strain belonged to genotype I and was close to those previously isolated in Indonesia. The results indicate that the dengue outbreak was caused mainly by DENV-3, with DENV-1 and DENV-2 as minor serotypes, and suggest that these strains of 3 serotypes of DENV entered East Timor from neighboring countries, co-circulated, and caused the dengue outbreak in 2005.
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.
authors.forename: Mikako
authors.initials: M
authors.lastname: Ito
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
authors.forename: Akira
authors.initials: A
authors.lastname: Kotaki
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tajima
authors.forename: Djoko
authors.initials: D
authors.lastname: Yuwono
authors.forename: Hem Sagar
authors.initials: HS
authors.lastname: Rimal
authors.forename: Filipe
authors.initials: F
authors.lastname: dos Santos
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: de Jesus
authors.forename: Bernadina Bano
authors.initials: BB
authors.lastname: Lina
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Tsuda
authors.forename: Chang-Kweng
authors.initials: CK
authors.lastname: Lim
authors.forename: Reiko
authors.initials: R
authors.lastname: Nerome
authors.forename: Antonio
authors.initials: A
authors.lastname: Calerés
authors.forename: Nahoko
authors.initials: N
authors.lastname: Shindo
authors.forename: Renu Dayal
authors.initials: RD
authors.lastname: Drager
authors.forename: Alex
authors.initials: A
authors.lastname: Andjaparidze
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003715:Dengue / Q000453:epidemiology* / Q000821:virology; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D010802:Phylogeny; D049648:Timor-Leste / Q000453:epidemiology