Role of angiogenic factors in acute experimental Strongyloides venezuelensis infection.
This study aims to investigate the role of angiogenic factors in the pathogenesis of experimental strongyloidiasis. Two complementary approaches were used: Firstly, CD1 mice were treated with endostatin, an angiogenesis inhibitor, and infected with Strongyloides venezuelensis. Also, the mechanisms involved in this process were studied. Parasitological examination revealed a significant decrease in egg per gram of faeces, number of collected larvae from lung tissue and number of collected adult females in mice treated with endostatin. Direct mechanisms with diminution of angiogenesis factors and an indirect mechanism with increase of eosinophil perhaps produced their effect. Secondly, the effect of the antigens responsible for stimulation of angiogenic factors [vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF2)] from alveolar macrophages and the mechanisms involved in their production were investigated. Alveolar macrophage cells obtained by bronchoalveolar lavage were incubated at different concentrations of somatic and excretory/secretory antigens of S. venezuelensis. Also, mRNA levels of VEGF and FGF2 in macrophage cells were detected by RT-PCR. L3-PBS larvae antigens induced angiogenic factors. The relationship between angiogenesis factors and nitric oxide has been observed using nitric oxide synthase inhibitors.
Shariati
F
F
Laboratorio de Inmunología y Parasitología Molecular, CIETUS, Facultad de Farmacia, Universidad de Salamanca, Salamanca, Spain.
Pérez-Arellano
J L
JL
López-Abán
J
J
El Behairy
A M
AM
Muro
A
A
D043925:Angiogenesis Inducing Agents / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D043169:Endostatins / Q000008:administration & dosage; D005243:Feces / Q000469:parasitology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D020869:Gene Expression Profiling; D008168:Lung / Q000469:parasitology; D016676:Macrophages, Alveolar / Q000276:immunology; D008297:Male; D051379:Mice; D010270:Parasite Egg Count; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013321:Strongyloides / Q000472:pathogenicity*; D013322:Strongyloidiasis / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
D043925:Angiogenesis Inducing Agents; D043169:Endostatins; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; C467498:vascular endothelial growth factor A, rat; D016222:Fibroblast Growth Factor 2