OCT3/4 regulates transcription of histone deacetylase 4 (Hdac4) in mouse embryonic stem cells.
OCT3/4 is a POU domain transcription factor that is critical for maintenance of pluripotency and self-renewal by embryonic stem (ES) cells and cells of the early mammalian embryo. It has been demonstrated to bind and regulate a number of genes, often in conjunction with the transcription factors SOX2 and NANOG. In an effort to further understand this regulatory network, chromatin immunoprecipitation was used to prepare a library of DNA segments specifically bound by OCT3/4 in undifferentiated mouse ES (mES) cell chromatin. One segment corresponds to a region within the first intron of the gene encoding histone deacetylase 4 (Hdac4), a Class II histone deacetylase. This region acts as a transcriptional repressor and contains at least two functional sites that are specifically bound by OCT3/4. HDAC4 is not expressed in the nuclei of OCT3/4+ mES cells and is upregulated upon differentiation. These findings demonstrate the participation of OCT3/4 in the repression of Hdac4 in ES cells.
Addis
Russell C
RC
Department of Cell and Developmental Biology, and Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Prasad
Megana K
MK
Yochem
Robert L
RL
Zhan
Xiangcan
X
Sheets
Timothy P
TP
Axelman
Joyce
J
Patterson
Ethan S
ES
Shamblott
Michael J
MJ
D000818:Animals; D001665:Binding Sites; D002843:Chromatin; D004247:DNA / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics; D053263:Gene Regulatory Networks; D006655:Histone Deacetylases / Q000235:genetics*; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors; D014158:Transcription, Genetic
D002843:Chromatin; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D014157:Transcription Factors; D004247:DNA; C498618:Hdac5 protein, mouse; D006655:Histone Deacetylases