Protective effect of chokeberry on chemical-induced oxidative stress in rat.
Male Wistar rats were treated with chokeberry juice per os, 10 mL/kg/day, for 28 days and a single intraperitoneal (i.p.) dose of N-nitrosodiethylamine (NDEA), 150 mg/kg, or carbon tetrachloride (CCl(4)), 2 ml/kg. The level of hepatic microsomal lipid peroxidation, expressed as thiobarbituric acid reactive substances (TBARS), was increased in animals dosed with NDEA and CCl(4). Juice pretreatment resulted in a significant decrease in TBARS by 53% and 92%, respectively. In rats administered juice alone, 50% decrease in TBARS was noted. The activities of all antioxidant enzymes were decreased in the liver of rats administered either toxicant by 29%-52% as compared to controls. Juice pretreatment resulted in an increase in the activity of catalase, glutathione peroxidase and glutathione reductase by 117%, 56% and 44%, respectively, only in rats challenged with NDEA. Although no response of plasma protein carbonyls to both toxicants was observed, the pretreatment with juice caused a 55% decrease of this parameter in CCl(4)-dosed rats. DNA damage in blood leukocytes induced by either toxicant was slightly reduced, by 24%, in the rats pretreated with juice and administered NDEA. The results of the study showed that pretreatment with chokeberry juice confers some protection against chemical-induced oxidative stress.
Kujawska
Małgorzata
M
Department of Toxicology, Poznań University of Medical Sciences, Poznań, Poland.
Ignatowicz
Ewa
E
Ewertowska
Małgorzata
M
Oszmiański
Jan
J
Jodynis-Liebert
Jadwiga
J
D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D004052:Diethylnitrosamine / Q000633:toxicity*; D004334:Drug Administration Schedule; D005638:Fruit / Q000737:chemistry*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D046936:Photinia / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D010936:Plant Extracts; D004052:Diethylnitrosamine; D002251:Carbon Tetrachloride