title: Retrograde axonal transport after radioactive serotonin injections into the olfactory bulb: a biochemical analysis of transported radioactive material.
abstract: A biochemical analysis of radioactive compounds was performed in the olfactory bulb (OB) and raphe dorsalis (RD) after injection of radioactive [(3)H] or [(14)C]serotonin (5-HT ranging from 10(?2) M to 10(?7) M) into the OB of rats treated or not with a monoamine-oxidase inhibitor (MAOI). In the OB of untreated rats, radioactivity was associated with precipitated protein and soluble perchloric acid (PCA) fractions. High performance liquid chromatography (HPLC) analysis of the PCA-supernatant gave 4 radioactive peaks: one associated with endogenous 5-HT, another with endogenous 5-hydroxyindole acetic acid (5-HIAA) and two without any relationship with endogenous hydroxyindoles: a '5-HT derivative A' and a '5-HT derivative B'. The presence of these '5-HT derivatives' was significantly reduced after treatment with 5,6-dihydroxytryptamine. In the RD, radioactivity was associated with the protein fraction and with '5-HT derivative A'. The kinetic analysis (from 30 min to 46 h) of the '5-HT derivative A' was characterized by a disappearance in the OB and an accumulation in the RD corresponding to a rate of migration in a range of 0.7 to 2 mm/h. This compound was absent or negligible in other non-serotoninergic neurons (such as the Locus Coeruleus, Amygdala and Cortex piriformis). No clear evidence for retrograde transport of radioactive 5-hydroxytryptophan (5-HTP) or 5-HIAA was found. At lower concentration of 5-HT injected into the OB, the half lives and the times of maximal accumulation for 5-HIAA, '5-HT derivative A' and '5-HT derivative B' were increased. The specific activity of 5-HT and 5-HIAA was also increased. The selective radioactive accumulation in the cell bodies of RD neurons after injection of radioactive 5-HT into the OB is discussed as resulting from a selectivity in (a) the uptake by 5-HT nerve terminals; (b) the metabolism of 5-HT into '5-HT derivative A' in the OB; (c) the retrograde axonal transport of '5-HT derivative A'. This '5-HT derivative A' could represent a messenger between nerve terminals and cell bodies and could be involved in homeostatic mechanisms that maintain cellular dynamics. When a MAOI was used, '5-HT-derivative A' and [(3)H]5-HT were found in the OB and also in the RD cell bodies, and to a lesser extent, in the non-serotoninergic cell bodies. These results indicate that MAO inhibition produces a relative non-selectivity in the 'uptake-metabolism and retrograde axonal transport' systems.
authors.affiliation: INSERM U 171, Laboratoire de Médecine Expérimentalle, Université Claude Bernard, 8, avenue Rockefeller, 6973 Lyon Cedex 2, France.
authors.forename: S
authors.initials: S
authors.lastname: Araneda
authors.forename: A
authors.initials: A
authors.lastname: Mermet
authors.forename: M
authors.initials: M
authors.lastname: Buda
authors.forename: P
authors.initials: P
authors.lastname: Bobillier
authors.forename: J F
authors.initials: JF
authors.lastname: Pujol