J774 macrophage-like cell line cytokine and chemokine patterns are modulated by Francisella tularensis LVS strain infection.
Mutual interactions were investigated between intracellular parasitic bacterium Francisella tularensis (F.t.; highly virulent bacterium responsible for tularemia, replicating within the host macrophages) and murine macrophage-like cell line J774. Recombinant murine lymphokine INF-gamma and/or LPS derived from E. coli were determined to stimulate in vitro antimicrobial activity of macrophage-like J774 cell line against the live vaccine strain (LVS) of F.t. through their ability to produce proinflammatory cytokines and chemokines. F.t. infection up-regulated IL-12 p40 production and down-regulated TNF-alpha production by stimulated macrophages; on the other hand, F.t. infection did not affect the production of IL-8, IL-6, MCP-5, and RANTES by stimulated macrophages. This showed that F.t. infection modulates the cytokine synthesis by J774 macrophage cell line.
Holicka
M
M
Department of Clinical Immunology and Allergology, University Hospital and School of Medicine in Hradec Kralove, Charles University in Prague, 500 05, Hradec Kralove, Czech Republic.
Novosad
J
J
Kudlova
M
M
Loudova
M
M
Andrys
C
C
Krejsek
J
J
D000818:Animals; D002460:Cell Line; D018925:Chemokines / Q000276:immunology; D016207:Cytokines / Q000276:immunology*; D005604:Francisella tularensis / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D014406:Tularemia / Q000276:immunology* / Q000382:microbiology
D018925:Chemokines; D016207:Cytokines