title: Chronic variable stress impairs energy metabolism in prefrontal cortex and hippocampus of rats: prevention by chronic antioxidant treatment.
abstract: Since chronic stress has been used widely for studying clinical depression and that brain energy metabolism and oxidative stress might be involved in the pathophysiology of this illness, the objective of this study was investigate the activities of pyruvate kinase, complex II and IV (cytocrome c oxidase) in hippocampus and prefrontal cortex of rats submitted to chronic variable stress. We also evaluated if vitamins E and C administration could prevent such effects. During 40 days adult rats from the stressed group were subjected to one stressor per day, at a different time each day, in order to minimize predictability. The stressed group had gained less weight while its immobilization time in the forced swimming test was greater than that of the control group. Results showed that stressed group presented an inhibition in the activities of complex II and cytochrome c oxidase in prefrontal cortex, while in hippocampus just complex IV was inhibited. Pyruvate kinase activity was not altered in stressed group when compared to control. Vitamins E and C administration prevented the alterations on respiratory chain caused by stress. These data suggest that the impairment of energy metabolism and oxidative stress could be related with the pathogenic pathways in stress related disorders.
authors.affiliation: Laboratório de Neuroproteção e Doenças Metabólicas, Porto Alegre, RS, CEP 90035-003, Brasil.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Tagliari
authors.forename: Cristie G
authors.initials: CG
authors.lastname: Noschang
authors.forename: Andréia G K
authors.initials: AG
authors.lastname: Ferreira
authors.forename: Otávio A
authors.initials: OA
authors.lastname: Ferrari
authors.forename: Luciane R
authors.initials: LR
authors.lastname: Feksa
authors.forename: Clovis M D
authors.initials: CM
authors.lastname: Wannmacher
authors.forename: Carla
authors.initials: C
authors.lastname: Dalmaz
authors.forename: Angela T S
authors.initials: AT
authors.lastname: Wyse
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000494:pharmacology / Q000627:therapeutic use; D001928:Brain Diseases, Metabolic / Q000188:drug therapy* / Q000209:etiology; D002908:Chronic Disease; D004195:Disease Models, Animal; D004579:Electron Transport / Q000187:drug effects / Q000502:physiology; D003576:Electron Transport Complex IV / Q000187:drug effects / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects* / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D017397:Prefrontal Cortex / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000150:complications / Q000378:metabolism* / Q000517:prevention & control*; D014810:Vitamin E / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D003576:Electron Transport Complex IV; D011770:Pyruvate Kinase; D001205:Ascorbic Acid