title: Chemical aminoacylation of tRNAs with fluorinated amino acids for in vitro protein mutagenesis.
abstract: This article describes the chemical aminoacylation of the yeast phenylalanine suppressor tRNA with a series of amino acids bearing fluorinated side chains via the hybrid dinucleotide pdCpA and ligation to the corresponding truncated tRNA species. Aminoacyl-tRNAs can be used to synthesize biologically relevant proteins which contain fluorinated amino acids at specific sites by means of a cell-free translation system. Such engineered proteins are expected to contribute to our understanding of discrete fluorines' interaction with canonical amino acids in a native protein environment and to enable the design of fluorinated proteins with arbitrary desired properties.
authors.affiliation: Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Institute of Chemistry and Biochemistry - Organic Chemistry, Takustrasse 3, 14195 Berlin, Germany.
authors.forename: Shijie
authors.initials: S
authors.lastname: Ye
authors.forename: Allison
authors.initials: A
authors.lastname: Ann Berger
authors.forename: Dominique
authors.initials: D
authors.lastname: Petzold
authors.forename: Oliver
authors.initials: O
authors.lastname: Reimann
authors.forename: Benjamin
authors.initials: B
authors.lastname: Matt
authors.forename: Beate
authors.initials: B
authors.lastname: Koksch