Characterization of cuticular chitin-binding proteins of Leptinotarsa decemlineata (Say) and post-ecdysial transcript levels at different developmental stages.
Seven cuticle chitin-binding proteins (Ld-CP1v1 to 7) were deduced from antenna cDNAs of adult Colorado potato beetles, Leptinotarsa decemlineata (Say), based on their consensus sequences. The mature proteins consisted of 87-188 residues. Ld-CP1v1 formed a distinct orthologous protein cluster (OP1) along with four proteins from other insect species in a neighbor-joining phylogenetic tree. These proteins also contained a proline glutamine-rich (PQ-rich) region and a highly conserved C-terminal motif (Phr). Their consensus region lacked the defined aromatic triad. Ld-CP2 to 6 clustered with those bearing RR-1 consensus and Ld-CP7 with RR-2 consensus. Ld-CP1v1 to 4 were expressed at the post-ecdysial period in all the developmental stages whereas Ld-CP5 to 7 were expressed mainly in adults.
Zhang
J
J
Potato Research Center, Agriculture and Agri-Food Canada, Fredericton, New Brunswick, Canada.
Pelletier
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D002686:Chitin / Q000378:metabolism*; D016000:Cluster Analysis; D001517:Coleoptera / Q000235:genetics* / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D034582:Integumentary System / Q000502:physiology*; D008969:Molecular Sequence Data; D018978:Molting / Q000235:genetics*; D010802:Phylogeny; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D019476:Insect Proteins; D012333:RNA, Messenger; D002686:Chitin