title: Fall in implantation rates following ICSI with sperm with high DNA fragmentation.
abstract: BACKGROUND
  There is considerable uncertainty as to the significance of a high sperm DNA fragmentation index (DFI) for achieving a successful pregnancy.
  
  
  METHODS
  The sperm DFI of 124 patients undergoing 192 IVF cycles and of 96 patients undergoing 155 ICSI cycles was determined using the sperm chromatin structure assay on neat sperm.
  
  
  RESULTS
  The rate of continuing pregnancies in ICSI cycles (but not in IVF cycles) showed significant negative correlation (r = -0.184, P = 0.022) with the DFI value. A threshold value of DFI which showed a significant difference (P = 0.005) in rate of continuing pregnancies between higher and lower DFI levels was found for ICSI cycles to be > or = 19%, but no such threshold was found for IVF cycles. However, if the threshold of > or = 30% was used for IVF cycles there was a non-significant lowering of the rates of continuing pregnancy and implantation at the higher DFI levels. DFI level had no effect on fertilization rate or on the percentage of embryos having more than 4 cells at Day 3 after fertilization. A high DFI level had a marked significant effect (P = 0.001) on implantation rate in ICSI cycles but not in IVF cycles. A significant positive correlation (r = 0.268, P = 0.001) between DFI and sperm midpiece defects was also noted in the ICSI patients.
  
  
  CONCLUSIONS
  These observations may help to resolve the issues about how, and to what extent, sperm DNA damage impacts upon the success of IVF and ICSI procedures.
authors.affiliation: Maternal & Fetal Medicine, IFWH, University College London, 86-96 Chenies Mews, London WC1E 6HX, UK. barbara.smith@ucl.ac.uk
authors.forename: B E
authors.initials: BE
authors.lastname: Speyer
authors.forename: A R
authors.initials: AR
authors.lastname: Pizzey
authors.forename: M
authors.initials: M
authors.lastname: Ranieri
authors.forename: R
authors.initials: R
authors.lastname: Joshi
authors.forename: J D A
authors.initials: JD
authors.lastname: Delhanty
authors.forename: P
authors.initials: P
authors.lastname: Serhal
mesh_terms: D000328:Adult; D053938:DNA Fragmentation; D010064:Embryo Implantation / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure