WITHDRAWN: A phosphate transporter from the root endophytic fungus Piriformospora indica plays a role in phosphate transport to the host plant.
Because pure cultures and a stable transformation system are not available for arbuscular mycorrhizal fungi, the role of their phosphate transporters for the symbiotic interaction with the plant up till now could not be studied. Here we report the cloning and the functional analysis of a gene encoding a phosphate transporter (PiPT) from the root endophytic fungus Piriformospora indica, which can be grown axenically. The PiPT polypeptide belongs to the major facilitator superfamily. Homology modeling reveals that PiPT exhibits twelve transmembrane helices divided into two halves connected by a large hydrophilic loop in the middle. The function of the protein encoded by PiPT was confirmed by complementation of a yeast phosphate transporter mutant. The kinetic analysis of PiPT (K(m) 25 mum) reveals that it belongs to the high affinity phosphate transporter family (Pht1). Expression of PiPT was localized to the external hyphae of P. indica colonized with maize plant root, which suggests that external hyphae are the initial site of phosphate uptake from the soil. To understand the physiological role of PiPT, knockdown transformants of the gene were prepared using electroporation and RNA interference. Knockdown transformants transported a significantly lower amount of phosphate to the host plant than wild-type P. indica. Higher amounts of phosphate were found in plants colonized with wild-type P. indica than that of non-colonized and plants colonized with knockdown PiPT P. indica. These observations suggest that PiPT is actively involved in the phosphate transportation and, in turn, P. indica helps improve the nutritional status of the host plant.
Yadav
Vikas
V
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Kumar
Manoj
M
Deep
Deepak Kumar
DK
Kumar
Hemant
H
Sharma
Ruby
R
Tripathi
Takshashila
T
Tuteja
Narendra
N
Saxena
Ajay Kumar
AK
Johri
Atul Kumar
AK
D001487:Basidiomycota / Q000737:chemistry*; D001692:Biological Transport; D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000235:genetics; D025301:Hyphae; D007700:Kinetics; D028061:Phosphate Transport Proteins / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D018517:Plant Roots / Q000382:microbiology*; D011487:Protein Conformation; D013559:Symbiosis / Q000502:physiology*; D003313:Zea mays / Q000382:microbiology
D028061:Phosphate Transport Proteins; D010710:Phosphates