Anti-inflammatory effect of enzymatic hydrolysate of corn gluten in an experimental model of colitis.
OBJECTIVE
Intestinal bacteria are thought to be involved in the initiation and perpetuation of inflammatory bowel diseases. Prebiotics (non-digestable dietary carbohydrate) have beneficial properties that alter the intestinal flora and contain glutamine-rich protein. Glutamine significantly decreases indices of inflammation. In this study, an enzymatic hydrolysate of corn gluten (EHCG) was administered by gavage to Sprague-Dawley rats fed an elemental diet to determine whether EHCG can ameliorate experi- mental colitis.


METHODS
Colitis was induced by intrarectal administration of 2,4,6-trinitrobenzene sulfonic acid after 10 days' daily oral administration of EHCG at 100 and 300 mg/kg. Macroscopic damage was assessed using a scoring system. The mucosa homogenate was sonicated and myeloperoxidase activity and histamine levels measured.


RESULTS
Treatment with EHCG significantly decreased the severity of injury and reduced myeloperoxidase activity and histamine levels in the distal colon mucosa.


CONCLUSIONS
EHCG may have therapeutic benefit as a supplement in enteral nutrition for patients with inflammatory bowel diseases.
Mochizuki
Miyako
M
Department of Human Life Science, Graduate School of Human Life Science, Gifu Women's University, Gifu, Japan.
Shigemura
Hayato
H
Hasegawa
Noboru
N
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000627:therapeutic use*; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D044683:Colon, Descending / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005526:Food, Formulated / Q000009:adverse effects; D005983:Glutens / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D011492:Protein Hydrolysates / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry*; D012720:Severity of Illness Index; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity; D003313:Zea mays / Q000737:chemistry*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011492:Protein Hydrolysates; D005983:Glutens; D006632:Histamine; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase