Validation of dot blot hybridization and denaturing high performance liquid chromatography as reliable methods for TP53 codon 72 genotyping in molecular epidemiologic studies.
BACKGROUND
Mutations in TP53 are common events during carcinogenesis. In addition to gene mutations, several reports have focused on TP53 polymorphisms as risk factors for malignant disease. Many studies have highlighted that the status of the TP53 codon 72 polymorphism could influence cancer susceptibility. However, the results have been inconsistent and various methodological features can contribute to departures from Hardy-Weinberg equilibrium, a condition that may influence the disease risk estimates. The most widely accepted method of detecting genotyping error is to confirm genotypes by sequencing and/or via a separate method.


RESULTS
We developed two new genotyping methods for TP53 codon 72 polymorphism detection: Denaturing High Performance Liquid Chromatography (DHPLC) and Dot Blot hybridization. These methods were compared with Restriction Fragment Length Polymorphism (RFLP) using two different restriction enzymes. We observed high agreement among all methodologies assayed. Dot-blot hybridization and DHPLC results were more highly concordant with each other than when either of these methods was compared with RFLP.


CONCLUSIONS
Although variations may occur, our results indicate that DHPLC and Dot Blot hybridization can be used as reliable screening methods for TP53 codon 72 polymorphism detection, especially in molecular epidemiologic studies, where high throughput methodologies are required.
Rabachini
Tatiana
T
Ludwig Institute for Cancer Research, São Paulo, Brazil.
Trottier
Helen
H
Franco
Eduardo L
EL
Villa
Luisa L
LL
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003062:Codon; D005260:Female; D016158:Genes, p53; D005838:Genotype; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D017720:Molecular Epidemiology / Q000379:methods; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
D003062:Codon