Bacillus nanhaiensis sp. nov., isolated from an oyster.
A novel Gram-stain-positive, slightly halophilic, facultatively alkaliphilic, catalase-positive, oxidase-negative, endospore-forming, motile, rod-shaped, aerobic bacterium, designated strain JSM 082006(T), was isolated from an oyster collected from Naozhou Island in the South China Sea. The isolate grew in 0-18 % (w/v) NaCl (optimum, 0.5-4.0 %), at pH 6.0-10.5 (optimum, pH 8.0) and at 15-45 °C (optimum, 30 °C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and C(16 : 0). Strain JSM 082006(T) contained MK-7 as the predominant respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The genomic DNA G+C content was 40.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 082006(T) should be assigned to the genus Bacillus and that it was most closely related to the type strains of Bacillus barbaricus (sequence similarity 99.1 %) and Bacillus arsenicus (97.5 %), followed by those of Bacillus rigui (96.6 %) and Bacillus solisalsi (96.1 %). Phylogenetic analysis, DNA-DNA relatedness values, phenotypic characteristics and chemotaxonomic data support the view that strain JSM 082006(T) represents a novel species of the genus Bacillus, for which the name Bacillus nanhaiensis sp. nov. is proposed; the type strain is JSM 082006(T) ( = DSM 23009(T)  = KCTC 13712(T)).
Chen
Yi-Guang
YG
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.|College of Biology and Environmental Sciences, Jishou University, Jishou 416000, PR China.
Zhang
Li
L
College of Biology and Environmental Sciences, Jishou University, Jishou 416000, PR China.
Zhang
Yu-Qin
YQ
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
He
Jian-Wu
JW
College of Biology and Environmental Sciences, Jishou University, Jishou 416000, PR China.
Klenk
Hans-Peter
HP
Deutsche Sammlung von Mikroorganismen und Zellkulturen, D-38124 Braunschweig, Germany.
Tang
Shu-Kun
SK
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Zhang
You-Xiang
YX
College of Biology and Environmental Sciences, Jishou University, Jishou 416000, PR China.
Li
Wen-Jun
WJ
Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.|The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
D000332:Aerobiosis; D000468:Alkalies / Q000378:metabolism; D000818:Animals; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010125:Ostreidae / Q000382:microbiology*; D010088:Oxidoreductases / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013171:Spores, Bacterial / Q000166:cytology; D013696:Temperature
D000468:Alkalies; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D003960:Diaminopimelic Acid; D010088:Oxidoreductases; D002374:Catalase