title: Dopamine stimulation of postnatal murine subventricular zone neurogenesis via the D3 receptor.
abstract: We investigated the expression and role of the dopamine receptor 3 (D3R) in postnatal mouse subventricular zone (SVZ). In situ hybridization detected selective D3R mRNA expression in the SVZ. Fluorescence activated cell sorting (FACS) of adult SVZ subtypes using hGFAP-GFP and Dcx-GFP mice showed that transit amplifying progenitor cells and niche astrocytes expressed D3R whereas stem cell-like astrocytes and neuroblasts did not. To determine D3R's role in SVZ neurogenesis, we administered U-99194A, a D3R preferential antagonist, and bromodeoxyuridine in postnatal mice. In vivo D3R antagonism decreased the numbers of newborn neurons reaching the core and the periglomerular layer of the olfactory bulb. Moreover, it decreased progenitor cell proliferation but did not change the number of label-retaining (stem) cells, commensurate with its expression on transit amplifying progenitor cells but not SVZ stem cell-like astrocytes. Collectively, this study suggests that dopaminergic stimulation of D3R drives proliferation via rapidly amplifying progenitor cells to promote murine SVZ neurogenesis.
authors.affiliation: Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
authors.forename: Yongsoo
authors.initials: Y
authors.lastname: Kim
authors.forename: Wei-Zhi
authors.initials: WZ
authors.lastname: Wang
authors.forename: Isabelle
authors.initials: I
authors.lastname: Comte
authors.forename: Erika
authors.initials: E
authors.lastname: Pastrana
authors.forename: Phuong B
authors.initials: PB
authors.lastname: Tran
authors.forename: Jennifer
authors.initials: J
authors.lastname: Brown
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Miller
authors.forename: Fiona
authors.initials: F
authors.lastname: Doetsch
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Molnár
authors.forename: Francis G
authors.initials: FG
authors.lastname: Szele
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D004298:Dopamine / Q000378:metabolism / Q000502:physiology*; D000089662:Doublecortin Protein; D020547:Lateral Ventricles / Q000166:cytology* / Q000254:growth & development*; D051379:Mice; D008822:Mice, Transgenic; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D050637:Receptors, Dopamine D3 / Q000235:genetics / Q000502:physiology*; D013234:Stem Cells / Q000378:metabolism* / Q000502:physiology
chemical_list: C000714847:Dcx protein, mouse; D000089662:Doublecortin Protein; D050637:Receptors, Dopamine D3; D004298:Dopamine