Antihyperglycemic effect of protocatechuic acid on streptozotocin-diabetic rats.
Protocatechuic acid (PCA) (3,4-dihydroxybenzoic acid), a natural phenolic compound found in many edible and medicinal plants, is a major benzoic acid derivative with a strong antioxidative effect, 10-fold higher than that of alpha-tocopherol. The present study is aimed at evaluating the antidiabetic effect of PCA on STZ-diabetic rats. Diabetes was induced in male albino Wistar rats by the administration of STZ (40 mg/kg BW, i.p.). PCA was administered orally at three different doses (50, 100, 200 mg/kg BW/day) to STZ-diabetic rats for 45 days. Diabetic rats showed increase in plasma glucose and glycosylated hemoglobin (HbA1c) and a decrease in plasma insulin and hemoglobin (Hb). The activities of gluconeogenic enzymes like glucose 6-phosphatase and fructose 1,6-bisphosphatase increased whereas the glycolytic enzyme glucokinase decreased in the liver along with glycogen content. The oral administration of PCA or glibenclamide in saline, for 45 days, prevented the changes and improved toward normalcy. No significant effect was observed in normal rats treated with PCA. Thus, our results show that PCA at 100 mg possesses a potential antihyperglycemic effect that is comparable with glibenclamide.
Harini
Ranganathan
R
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar - 608 002, Tamilnadu, India.
Pugalendi
Kodukkur Viswanathan
KV
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D005905:Glyburide / Q000627:therapeutic use; D006442:Glycated Hemoglobin / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D062385:Hydroxybenzoates / Q000627:therapeutic use*; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D007328:Insulin / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D010179:Pancreas / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D005905:Glyburide; D006442:Glycated Hemoglobin; D006454:Hemoglobins; D062385:Hydroxybenzoates; D007004:Hypoglycemic Agents; D007328:Insulin; D008112:Liver Glycogen; C009091:protocatechuic acid