Immobilization of type-I collagen and basic fibroblast growth factor (bFGF) onto poly (HEMA-co-MMA) hydrogel surface and its cytotoxicity study.
Type-I collagen and bFGF were immobilized onto the surface of poly (HEMA-co-MMA) hydrogel by grafting and coating methods to improve its cytotoxicity. The multi-layered structure of the biocompatible layer was confirmed by FTIR, AFM and static water contact angles. The layers were stable in body-like environment (pH 7.4). Human skin fibroblast cells (HSFC) were seeded onto Col/bFGF-poly (HEMA-co-MMA), Col-poly (HEMA-co-MMA) and poly (HEMA-co-MMA) films for 1, 3 and 5 day. MTT assay was performed to evaluate the extraction toxicity of the materials. Results showed that the cell attachment, proliferation and differentiation on Col/bFGF-poly (HEMA-co-MMA) film were higher than those of the control group, which indicated the improvement of cell-material interaction. The extraction toxicity of the modified materials was also lower than that of the unmodified group. The protein and bFGF immobilized poly (HEMA-co-MMA) hydrogel might hold great promise to be a biocompatible material.
Yan
Tuo
T
Department of Biomedical Engineering, Jinan University, Guangzhou, China. hgyant@163.com
Sun
Rong
R
Li
Chun
C
Tan
Baihua
B
Mao
Xuan
X
Ao
Ningjian
N
D000327:Adsorption; D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D024042:Collagen Type I / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D004355:Drug Stability; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology / Q000633:toxicity*; D008422:Materials Testing; D008768:Methylmethacrylates / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008954:Models, Biological; D011102:Polyhydroxyethyl Methacrylate / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D013499:Surface Properties; D018675:Toxicity Tests
D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D008768:Methylmethacrylates; D016222:Fibroblast Growth Factor 2; D011102:Polyhydroxyethyl Methacrylate; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C040206:HTR composite