title: The most common Chinese rhesus macaque MHC class I molecule shares peptide binding repertoire with the HLA-B7 supertype.
abstract: Of the two rhesus macaque subspecies used for AIDS studies, the Simian immunodeficiency virus-infected Indian rhesus macaque (Macaca mulatta) is the most established model of HIV infection, providing both insight into pathogenesis and a system for testing novel vaccines. Despite the Chinese rhesus macaque potentially being a more relevant model for AIDS outcomes than the Indian rhesus macaque, the Chinese-origin rhesus macaques have not been well-characterized for their major histocompatibility complex (MHC) composition and function, reducing their greater utilization. In this study, we characterized a total of 50 unique Chinese rhesus macaques from several varying origins for their entire MHC class I allele composition and identified a total of 58 unique complete MHC class I sequences. Only nine of the sequences had been associated with Indian rhesus macaques, and 28/58 (48.3%) of the sequences identified were novel. From all MHC alleles detected, we prioritized Mamu-A1*02201 for functional characterization based on its higher frequency of expression. Upon the development of MHC/peptide binding assays and definition of its associated motif, we revealed that this allele shares peptide binding characteristics with the HLA-B7 supertype, the most frequent supertype in human populations. These studies provide the first functional characterization of an MHC class I molecule in the context of Chinese rhesus macaques and the first instance of HLA-B7 analogy for rhesus macaques.
authors.affiliation: Department of Biological Sciences, California State University - San Marcos, San Marcos, CA 92096, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Solomon
authors.forename: Scott
authors.initials: S
authors.lastname: Southwood
authors.forename: Ilka
authors.initials: I
authors.lastname: Hoof
authors.forename: Richard
authors.initials: R
authors.lastname: Rudersdorf
authors.forename: Bjoern
authors.initials: B
authors.lastname: Peters
authors.forename: John
authors.initials: J
authors.lastname: Sidney
authors.forename: Clemencia
authors.initials: C
authors.lastname: Pinilla
authors.forename: Maria Cecilia Garibaldi
authors.initials: MC
authors.lastname: Marcondes
authors.forename: Binhua
authors.initials: B
authors.lastname: Ling
authors.forename: Preston
authors.initials: P
authors.lastname: Marx
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sette
authors.forename: Bianca R
authors.initials: BR
authors.lastname: Mothé
mesh_terms: D000818:Animals; D002681:China; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D007194:India; D008253:Macaca mulatta / Q000235:genetics*; D010446:Peptide Fragments / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D015395:Histocompatibility Antigens Class I; D010446:Peptide Fragments