A novel concept of membrane reconstitution applied to acetylcholine receptor from Torpedo and matrix protein from escherichia coli.
Recently developed techniques to form and investigate lipid-protein planar membranes are shown to fulfill the basic requirements of membrane reconstitution. A novel concept and strategy for reconstitution is proposed including experimental assays. Application to matrix protein from E. coli outer membranes demonstrates that this concept allows reconstitution of electrical membrane processes without electrophysiological a priori data. Application to the acetylcholine receptor from Torpedo marmorata indicates that the technique of planar membrane formation conserves the essential properties of the in vivo receptor.
Schindler
H
H
Biozentrum, University of Basel, CH-4056 Basel, Switzerland.
Rosenbusch
J P
JP
Quast
U
U