title: Titanium immobilized with an antimicrobial peptide derived from histatin accelerates the differentiation of osteoblastic cell line, MC3T3-E1.
abstract: The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. The colonization of P. gingivalis on JH8194-Ti was significantly lower than that on control- and blocking-Ti. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control- and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration.
authors.affiliation: Division of Oral Health Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
authors.forename: Seicho
authors.initials: S
authors.lastname: Makihira
authors.affiliation: Division of Oral Health Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shuto
authors.affiliation: Division of Oral Health Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nikawa
authors.affiliation: Toyo Advanced Technologies Co., LTD. 5-3-38, Ujina-Higashi, Minami-ku, Hiroshima 734-8501, Japan.
authors.forename: Keishi
authors.initials: K
authors.lastname: Okamoto
authors.affiliation: Division of Oral Health Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Mine
authors.affiliation: Division of Oral Health Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takamoto
authors.affiliation: Hiroshima University Hospital, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Ohara
authors.affiliation: Two cell Co. LTD., 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Tsuji
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D054808:Histatins / Q000737:chemistry* / Q000378:metabolism; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D014025:Titanium / Q000737:chemistry*
chemical_list: D023181:Antimicrobial Cationic Peptides; D050659:Core Binding Factor Alpha 1 Subunit; D054808:Histatins; D055767:Immobilized Proteins; D012333:RNA, Messenger; C493716:Runx2 protein, mouse; D053495:Osteopontin; D014025:Titanium; D000469:Alkaline Phosphatase