title: Talaromyces emersonii thermostable enzyme systems and their applications in wheat baking systems.
abstract: In this study, novel extracellular thermozymes were produced by the thermophilic fungus Talaromyces emersonii (IMI 392299) on low-cost carbon inducers. This paper reports the cocktail characterization, substrate hydrolysis studies, and their application in baking. Relevant enzymes were optimally active at pH 4.5-5.0 and 70 degrees C. Model studies confirmed production of significant levels of yeast monosaccharide sugars during cereal flour hydrolysis. The "thermozyme cocktails" are thermostable secreted T. emersonii enzyme blends. In baking trials, these thermozyme cocktails showed significant improvements in bread quality with respect to hardness, staling, and loaf volume (p < 0.5). Thermozyme cocktail B- treated loaf volume was 23.2% greater than the control and 49.5% softer. Staling analysis showed that bread treated with cocktail B was 41.7% softer than the control. This is the first report of T. emersonii thermozymes positively influencing bread quality.
authors.affiliation: Molecular Glycobiotechnology Group, Biochemistry, School of Natural Sciences, National University of Ireland, Galway, Ireland. d.waters2@nuigalway.ie
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Waters
authors.forename: Patrick G
authors.initials: PG
authors.lastname: Murray
authors.forename: Liam A
authors.initials: LA
authors.lastname: Ryan
authors.forename: Elke K
authors.initials: EK
authors.lastname: Arendt
authors.forename: Maria G
authors.initials: MG
authors.lastname: Tuohy
mesh_terms: D001939:Bread / Q000032:analysis; D004795:Enzyme Stability; D005433:Flour / Q000032:analysis; D005511:Food Handling; D005656:Fungal Proteins / Q000737:chemistry*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D032901:Talaromyces / Q000201:enzymology*; D014908:Triticum / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins