title: Dynamics of fat absorption and effect of sham feeding on postprandial lipema.
abstract: OBJECTIVE
  Given the importance of postprandial hyperlipidemia to increase risk for atherosclerosis, in the present study, stable isotope-labeled meals were fed to healthy subjects (7 males and 3 females) to investigate the kinetics chylomicron synthesis and the effect of sensory exposure to lipid on metabolism.
  
  
  METHODS
  Subjects performed two, 24-hour inpatient studies that entailed consumption of a liquid formula evening meal containing 30 g of oil (+ (13)C(2) triolein) on day 1. Breakfast (day 2) consisted of triacylglycerols (TAGs) fed as capsules (30 g oil + (13)C(7) triolein) to avoid activation of mouth taste receptors. Next, modified sham feeding of cream cheese occurred over 2 hours. In the 2 trials, the stimulus was either higher fat (HF) or lower fat (LF) cream cheese. A liquid meal was consumed at lunch. Blood sampling occurred intermittently, and chylomicron particles S(f) >400 TAGs were analyzed by gas chromatography-mass spectrometry.
  
  
  RESULTS
  (13)C(2)-Label was found in fasting-state lipoproteins, and persons with higher body fat percentages showed greater dilution of meal TAGs from endogenous sources. For both trials, 13% ± 4% of lipoprotein TAGs oleic acid was derived from the previous evening meal. Incremental area under the curve for TAGs during HF was ∼2.5 times higher than after LF exposure (46 ± 15 vs 17 ± 5 μmol/L/h; P = .04). The greater HF morning lipemia occurred with elevated glucose, insulin, and nonesterified fatty acids peak after lunch.
  
  
  CONCLUSIONS
  These data support a connection between enteral lipid metabolism and oral fat exposure, resulting in elevated postprandial lipemia. The results suggest that the intestine may participate in a mechanism coordinating oral fat signaling with control of subsequent macronutrient disposal in the body.
authors.affiliation: Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9052, USA.
authors.forename: Rosa N
authors.initials: RN
authors.lastname: Chavez-Jauregui
authors.forename: Richard D
authors.initials: RD
authors.lastname: Mattes
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Parks
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D050197:Atherosclerosis / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D004041:Dietary Fats / Q000493:pharmacokinetics*; D020895:Enterocytes / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006949:Hyperlipidemias / Q000150:complications / Q000178:diet therapy / Q000378:metabolism*; D007408:Intestinal Absorption / Q000502:physiology*; D008055:Lipids / Q000097:blood*; D008297:Male; D019518:Postprandial Period; D011379:Prognosis; D012307:Risk Factors
chemical_list: D004041:Dietary Fats; D008055:Lipids