title: The Lucid Proteomics System for top-down biomarker research.
abstract: Advances have been made in recent years for both "top-down" and "bottom-up" profiling approaches to biomarker discovery. Top-down protein profiling via SELDI-TOF mass spectrometry has been used by researchers in many fields of study to discover native protein biomarker candidates from a variety of sample types, but has been limited without a means for straightforward identification of these candidates. Bio-Rad has recently partnered with Bruker Daltonics to create the Lucid Proteomics System, a complete SELDI-based research workflow--system qualification, biomarker discovery, data analysis, and biomarker purification/identification--using Bruker's flex series of TOF and TOF/TOF mass spectrometers, which have long provided consistent performance and high value data for MALDI applications. This collaboration enables both top-down and bottom-up proteomics approaches on a single high performance MALDI-TOF MS platform for maximum coverage of the proteome--allowing greater flexibility with experimental design and accelerating biomarker research programmes.
authors.affiliation: Bio-Rad Laboratories, Division Bio-Recherche, 3, Boulevard Raymond Poincaré, Marnes la Coquette 92430, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Jourdain
authors.forename: Amanda
authors.initials: A
authors.lastname: Bulman
authors.forename: Enrique
authors.initials: E
authors.lastname: Dalmasso
mesh_terms: D000465:Algorithms; D015415:Biomarkers / Q000032:analysis / Q000737:chemistry; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods*; D013223:Statistics as Topic
chemical_list: D015415:Biomarkers; D010446:Peptide Fragments; D011506:Proteins