Geographic distribution of phylogenetically-distinct legume pod borer, Maruca vitrata (Lepidoptera: Pyraloidea: Crambidae).
Maruca vitrata Fabricius is a pantropical lepidopteran pest of legumes. Phylogenetic analysis of a mitochondrial cytochrome c oxidase-I gene (cox1) fragment indicates that three Maruca sp. mitochondrial lineages have unique geographic distributions [lineages 1 and 2: Australia, Taiwan, and West Africa (Niger, Nigeria, and Burkina Faso), and lineage 3: Puerto Rico]. The haplotype (T30, T114) is specific to lineages 1&2 and was assayed by NsiI and SacI polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) within population samples; it was not observed in the Puerto Rican samples, but was nearly fixed among samples from West Africa, Australia and Taiwan (85.5-100%). Re-sequencing and phylogenetic analyses of PCR-RFLP defined cox1 haplotypes indicate that nucleotide diversity is highest among samples from West Africa. Phylogenetic reconstruction based upon ribosomal DNA (rDNA) internal transcribed spacer-2 (ITS-2) sequences provided additional evidence for three Maruca sp. clades. These data suggest that multiple unique Maruca species or subspecies are present worldwide, which has implications for the management of this pest species-complex.
Margam
Venu M
VM
Department of Entomology, Purdue University, 901 W State St., Smith Hall, W Lafayette, IN 47907, USA. vmargam@purdue.edu
Coates
Brad S
BS
Ba
Malick N
MN
Sun
Weilin
W
Binso-Dabire
Clementine L
CL
Baoua
Ibrahim
I
Ishiyaku
Mohammad F
MF
Shukle
John T
JT
Hellmich
Richard L
RL
Covas
Fernando G
FG
Ramasamy
Srinivasan
S
Armstrong
Joel
J
Pittendrigh
Barry R
BR
Murdock
Larry L
LL
D000349:Africa; D000818:Animals; D001315:Australia; D021901:DNA, Intergenic / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes; D007915:Lepidoptera / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D011647:Puerto Rico; D013624:Taiwan
D021901:DNA, Intergenic; D004275:DNA, Ribosomal