A method for simple and accurate identification of the multiple sclerosis associated allele HLA-DRB1*1501 in neuroscience research laboratories.
Research on multiple sclerosis (MS) frequently requires typing for allele HLA-DRB1*1501, which the complexities of the HLA system can restrict to specialised histocompatibility laboratories. To overcome this limitation, we have implemented a simple, robust and highly specific method for DRB1*1501 detection. One single-tube polymerase-chain reaction (PCR) per DNA sample allows for detecting DR2 individuals. The spare PCR products of these are then sequenced to identify allele DRB1*1501 by comparison with the official, publicly accessible HLA database. This approach, much simpler than previously available methods, should facilitate research on MS by making accurate identification of DRB1*1501 accessible to neuroscience laboratories.
Cisneros
E
E
Inmunogenética-HLA, Hospital Universitario Puerta de Hierro, Majadahonda, Spain.
Moraru
M
M
de Pablo
R
R
Vilches
C
C
D000483:Alleles; D030541:Databases, Genetic / Q000706:statistics & numerical data; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing / Q000379:methods*; D006684:HLA-DR Antigens / Q000235:genetics*; D015802:HLA-DR2 Antigen / Q000235:genetics; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D009103:Multiple Sclerosis / Q000175:diagnosis* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods
D006684:HLA-DR Antigens; D015802:HLA-DR2 Antigen; D059811:HLA-DRB1 Chains; C507799:HLA-DRB1*15 antigen