title: Biodistribution of Technetium-99m pertechnetate after total gastrectomy and Roux-en-Y jejunal pouch.
abstract: This paper investigates the influence of total gastrectomy, with and without a jejunal pouch, on the biodistribution of the radiopharmaceutical Technetium-99m pertechnetate ((99m)TcO(4)(-)). A quantitative biodistribution study was performed on the 30th postoperative day in Wistar rats, using the Wizard PerkinElmer gamma counter. The percentage of injected radioactivity of (99m)TcO(4)(-) per gram of tissue (% ATI/g) in Roux-en-Y total gastrectomy rats was lower in the lung, brain, and femur, compared to the sham rats (p <.05). In the jejunal pouch rats, the%ATI/g was higher than in the jejunum of sham rats (p <.05). The biodistribution was higher in the lung, brain, and femur of Roux-en-Y pouch rats than in Roux-en-Y rats (p <.05). Serum levels of cobalamin, phosphorus, and calcium were lower in gastrectomized than in sham rats (p <.05). No significant histological change was observed in the jejunum of Roux-en-Y rats. In the jejunal pouch, we found vascular ingurgitation, inflammation, submucosal, and muscular wall hypertrophy and widening of the villi. In conclusion, total gastrectomy in rats affected the biodistribution of (99m)TcO(4)(-) to important organs, mainly when a Roux-en-Y reconstruction without a pouch was performed. Moreover, the operation caused biodistribution-related metabolic changes.
authors.affiliation: Postgraduate Program in Health Sciences, Federal University of Rio Grande do Norte, Natal, Brazil.
authors.forename: Amália Cínthia Meneses
authors.initials: AC
authors.lastname: Rêgo
authors.forename: Arthur
authors.initials: A
authors.lastname: Villarim Neto
authors.forename: Italo Medeiros
authors.initials: IM
authors.lastname: Azevedo
authors.forename: Irami
authors.initials: I
authors.lastname: Araújo-Filho
authors.forename: Eryvaldo Sócrates Tabosa
authors.initials: ES
authors.lastname: Egito
authors.forename: Aldo Cunha
authors.initials: AC
authors.lastname: Medeiros
mesh_terms: D000713:Anastomosis, Roux-en-Y / Q000009:adverse effects*; D000818:Animals; D001921:Brain / Q000378:metabolism; D005269:Femur / Q000378:metabolism; D005743:Gastrectomy / Q000009:adverse effects*; D007583:Jejunum / Q000378:metabolism / Q000601:surgery*; D008168:Lung / Q000378:metabolism; D008297:Male; D019275:Radiopharmaceuticals; D051381:Rats; D017208:Rats, Wistar; D013670:Sodium Pertechnetate Tc 99m; D014018:Tissue Distribution
chemical_list: D019275:Radiopharmaceuticals; D013670:Sodium Pertechnetate Tc 99m