Influence of diurnal variation in mesophyll conductance on modelled 13C discrimination: results from a field study.
Mesophyll conductance to CO(2) (g(m)) limits carbon assimilation and influences carbon isotope discrimination (Delta) under most environmental conditions. Current work is elucidating the environmental regulation of g(m), but the influence of g(m) on model predictions of Delta remains poorly understood. In this study, field measurements of Delta and g(m) were obtained using a tunable diode laser spectroscope coupled to portable photosynthesis systems. These data were used to test the importance of g(m) in predicting Delta using the comprehensive Farquhar model of Delta (Delta(comp)), where g(m) was parameterized using three methods based on: (i) mean g(m); (ii) the relationship between stomatal conductance (g(s)) and g(m); and (iii) the relationship between time of day (TOD) and g(m). Incorporating mean g(m), g(s)-based g(m), and TOD-based g(m) did not consistently improve Delta(comp) predictions of field-grown juniper compared with the simple model of Delta (Delta(simple)) that omits fractionation factors associated with g(m) and decarboxylation. Sensitivity tests suggest that b, the fractionation due to carboxylation, was lower (25 per thousand) than the value commonly used in Delta(comp) (29 per thousand) and Delta(simple) (27 per thousand). These results demonstrate the limits of all tested models in predicting observed juniper Delta, largely due to unexplained offsets between predicted and observed values that were not reconciled in sensitivity tests of variability in g(m), b, or e, the day respiratory fractionation.
Bickford
Christopher P
CP
University of New Mexico, Department of Biology, MSC03-2020, Albuquerque, NM 87131, USA. christopher.p.bickford@gmail.com
Hanson
David T
DT
McDowell
Nate G
NG
D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes / Q000378:metabolism; D002940:Circadian Rhythm; D018653:Juniperus / Q000502:physiology*; D008954:Models, Biological; D010788:Photosynthesis; D054046:Plant Stomata / Q000502:physiology*; D013057:Spectrum Analysis
D002247:Carbon Isotopes; D002245:Carbon Dioxide