Val-9Ala and Ile+58Thr polymorphism of MnSOD in Parkinson's disease.
OBJECTIVE
To investigate the polymorphism distribution of Val-9Ala and Ile+58Thr of the Mn-superoxide dismutase (Mn-SOD) gene among subjects with Parkinson's disease (PD) by analyses of genders and clinical severity.


METHODS
We examined the DNA genotypes of Val-9Ala and Ile+58Thr from 295 PD subjects and 111 controls by nucleotide sequencing and BsaWI restriction.


RESULTS
Ala/Ala homozygosity was found in four PD subjects but not in the controls. All of the genotypes at codon +58 among the examined samples were Ile/Ile homozygotes. Although higher carrier rate of Ala allele among PD subjects than the controls, there were no differences by analyses of the genders and clinical severity.


CONCLUSIONS
The higher Ala-allele carrier rate among PD subjects may suggest a possible higher amount of mitochondrial Mn-SOD rendering higher intracellular stress in PD. In this study the polymorphisms at codons -9 and+58 did not give informative association evidences with PD.
Wang
Vinchi
V
Department of Neurology, Cardinal Tien Hospital, Taipei, Taiwan. vwneur@yahoo.com.tw
Chen
Shao-Yuan
SY
Chuang
Tzu-Chao
TC
Shan
Din-E
DE
Soong
Bing-Wen
BW
Kao
Ming-Ching
MC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000201:enzymology* / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics*
D013482:Superoxide Dismutase