title: Immunomodulatory activity of butanol extract from Solanum lyratum in tumor-bearing mice.
abstract: Solanum lyratum Thunb (Solanaceae) has been widely used for cancer as a folk remedy in Chinese traditional medicine. In this study, the main active fraction n-butanol extract from S. lyratum (BESL) was evaluated for the therapeutic efficacies on mice transplantable tumor and immunomodulatory potentials on the immune response in tumor-bearing mice. The effects of BESL on the growth of mouse transplantable S180 sarcoma, splenocyte proliferation, the activity of natural killer (NK) cells and cytotoxic T lymphocytes (CTL), production of cytokines from splenocytes, and serum antigen-specific antibody levels in S180-bearing mice were measured. BESL could not only significantly inhibit the growth of S180 sarcoma transplanted in mice, but also remarkably promote splenocytes proliferation, NK cell and CTL activity, interleukin-2 and interferon-γ production from splenocytes, and serum antigen-specific antibody levels in tumor-bearing mice (P < 0.05, P < 0.01, or P <0.001). The results suggested that BESL might exhibit antitumor activity by improving immune response, and it could act as antitumor agent with immunomodulatory activity. This study provided evidence to understand the therapeutic effects of S. lyratum for treatment of cancer and a natural product to further researches to be developed as a cancer chemopreventive agent.
authors.affiliation: The Third Hospital of Yuhang District, Hangzhou, Zhejiang, People's Republic of China. SH_Liu2010@163.com
authors.forename: Shu-hua
authors.initials: SH
authors.lastname: Liu
authors.forename: Xing-hang
authors.initials: XH
authors.lastname: Shen
authors.forename: Xian-feng
authors.initials: XF
authors.lastname: Wei
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Mao
authors.forename: Ting
authors.initials: T
authors.lastname: Huang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D000440:Butanols / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay; D056724:Immunity, Humoral / Q000187:drug effects; D007155:Immunologic Factors / Q000302:isolation & purification / Q000627:therapeutic use*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D035261:Plant Components, Aerial / Q000737:chemistry; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000276:immunology*; D032322:Solanum / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000906:Antibodies; D000970:Antineoplastic Agents; D000440:Butanols; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors