Amycolatopsis thailandensis sp. nov., a poly(L-lactic acid)-degrading actinomycete, isolated from soil.
A novel actinomycete that was capable of degrading poly(l-lactic acid), strain CMU-PLA07(T), was isolated from soil in northern Thailand. Strain CMU-PLA07(T) had biochemical, chemotaxonomic, morphological and physiological properties that were consistent with its classification in the genus Amycolatopsis. 16S rRNA gene sequence analysis showed that the isolate formed a phyletic line within the genus Amycolatopsis. Strain CMU-PLA07(T) was most similar to Amycolatopsis coloradensis IMSNU 22096(T) (99.5 % 16S rRNA gene sequence similarity) and Amycolatopsis alba DSM 44262(T) (99.4 %). However, strain CMU-PLA07(T) was distinguishable from the type strains of species of the genus Amycolatopsis on the basis of DNA-DNA relatedness and phenotypic data. Therefore, strain CMU-PLA07(T) is considered to represent a novel species of the genus Amycolatopsis, for which the name Amycolatopsis thailandensis sp. nov. is proposed. The type strain is CMU-PLA07(T) ( = JCM 16380(T) = BCC 38279(T)).
Chomchoei
Atchareeya
A
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Pathom-Aree
Wasu
W
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Yokota
Akira
A
Institute of Molecular and Cellular Bioresources, The University of Tokyo, Tokyo 113-0032, Japan.
Kanongnuch
Chartchai
C
Department of Biotechnology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50200, Thailand.
Lumyong
Saisamorn
S
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019344:Lactic Acid / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011091:Polyesters; D011108:Polymers / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013785:Thailand
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011091:Polyesters; D011108:Polymers; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; C033616:poly(lactide)