Estrogenic and anti-estrogenic effects of wood extractives present in pulp and paper mill effluents on rainbow trout.
Wood extractives present in pulp and paper mill effluents may cause reproductive disturbances in fish. A chronic-exposure toxicity experiment using immature rainbow trout (Oncorhynchus mykiss) was conducted in order to assess the endocrine disrupting effects of two Chilean pulp and paper mill specific extracts (solid phase extraction, SPE) obtained from primary and secondary treated effluents. The (anti)estrogenic potencies and toxicity of the wood extractives regularly present in pulp mill effluent such as dehydroabietic acid (DHAA), beta-sitosterol (BS), and model estrogen 17beta-estradiol (E2) were evaluated by analysis of plasma vitellogenin (VTG) levels, gonadal somatic index (GSI) and liver ethoxyresorufin-O-deethylase (EROD) activity, respectively. The protocol involved the use of multiple intra-peritoneal injections (1 injection every 7 days for a total exposure period of 28 days). Analysis of variance/covariance, demonstrated no differences associated with fish gender other than GSI. The phytosterol BS, E2 and both pulp mill effluent extracts showed significant inductions of EROD and increased VTG levels after 4, 7, 14, 21 and 28 of exposure. While fish injected with secondary treated effluent extract showed a delayed induction in VTG levels compared to primary effluent injected fish, no effects on VTG and EROD levels were observed in DHAA injected fish. Moreover simultaneous injection of DHAA+E2 reduced the VTG levels found in E2 injected fish, indicating a potential indirect anti-estrogenic effect of this resin acid. The results of this study indicate that Chilean pulp and paper mill effluent extracts are estrogenic in rainbow trout males and females.
Orrego
Rodrigo
R
University of Ontario Institute of Technology, Faculty of Science, 2000 Simcoe Street North, Oshawa, Ontario, Canada. Rodrigo.Orrego@uoit.ca
Guchardi
John
J
Krause
Rachelle
R
Holdway
Douglas
D
D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000097:blood; D052244:Endocrine Disruptors / Q000633:toxicity; D004958:Estradiol / Q000633:toxicity; D005260:Female; D007220:Industrial Waste; D008099:Liver / Q000187:drug effects*; D008297:Male; D017686:Oncorhynchus mykiss / Q000502:physiology*; D010209:Paper; D012855:Sitosterols / Q000633:toxicity; D014819:Vitellogenins / Q000097:blood; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014934:Wood / Q000737:chemistry*
D052244:Endocrine Disruptors; D007220:Industrial Waste; D012855:Sitosterols; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D004958:Estradiol; C025473:gamma-sitosterol; D019363:Cytochrome P-450 CYP1A1