title: [Preparation and application of polyclonal antibody of human transcription factor CTCF N terminus protein].
abstract: To investigate the function of CTCF and understand the pathogenesis of tumors better, we produced rabbit polyclonal antibody of human transcription factor CTCF protein and detected its expression in several kinds of human cancer cells and tissues. GST fusion protein of human CTCF-N domain was purified by GSTrap-FF affinity chromatography and was successfully expressed under induction of IPTG in E. coli BL21. Western blotting analysis demonstrated that the polyclonal antibody can recognize the endogenous CTCF from HepG2, MCF-7 and HeLa cells specifically. The produced antibodies can be used for gene expression regulation and tissue distribution study at protein level.
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.forename: Ling
authors.initials: L
authors.lastname: Wei
authors.forename: Xinjuan
authors.initials: X
authors.lastname: Chai
authors.forename: Yajun
authors.initials: Y
authors.lastname: Ge
authors.forename: Yang
authors.initials: Y
authors.lastname: Qin
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D000076246:CCCTC-Binding Factor; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*
chemical_list: D000906:Antibodies; D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D005982:Glutathione Transferase