Effect of losartan and benzbromarone on the level of human urate transporter 1 mRNA.
Both an angiotensin II receptor blocker, losartan (CAS 124750-99-8) and a serum urate lowering agent, benzbromarone (CAS 3562-84-3) exert a uricosuric action by inhibiting urate transporter 1 (URAT1). A recent clinical trial indicated that losartan could reduce the level of serum urate in hypertensive patients treated with urate lowering agents, suggesting the different mode of action of losartan from benzbromarone. In the present study, the effect of losartan and benzbromarone on the level of URAT1 mRNA was determined in transfected HEK293 cells. Losartan caused a significant reduction of its mRNA level, whereas it was not affected by benzbromarone. These results indicate that losartan decreases the level of human URAT1 mRNA, which may underlie the uricosuric action of losartan in hypertensive patients treated with serum urate lowering agents.
Nindita
Yora
Y
Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science, Yonago, Japan.
Hamada
Toshihiro
T
Bahrudin
Udin
U
Hosoyamada
Makoto
M
Ichida
Kimiyoshi
K
Iwai
Chisato
C
Urashima
Sunao
S
Kuwabara
Masanari
M
Kuwabara
Narimasa
N
Utami
Sulistiyati Bayu
SB
Mizuta
Einosuke
E
Yamada
Kensaku
K
Igawa
Osamu
O
Shigemasa
Chiaki
C
Ninomiya
Haruaki
H
Tsuchihashi
Takuya
T
Hisatome
Ichiro
I
D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology*; D001553:Benzbromarone / Q000494:pharmacology*; D002460:Cell Line; D006801:Humans; D007668:Kidney / Q000378:metabolism; D019808:Losartan / Q000494:pharmacology*; D027361:Organic Anion Transporters / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D027701:Organic Cation Transport Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014527:Uric Acid / Q000378:metabolism; D014528:Uricosuric Agents / Q000494:pharmacology*
D047228:Angiotensin II Type 1 Receptor Blockers; D027361:Organic Anion Transporters; D027701:Organic Cation Transport Proteins; D012333:RNA, Messenger; C491241:SLC22A12 protein, human; D014528:Uricosuric Agents; D014527:Uric Acid; D001553:Benzbromarone; D019808:Losartan