title: [Sequential preparation of microvlllous and basal membranes from human placenta].
abstract: OBJECTIVE
  To improve the technology of isolating paired fractions of the maternal-facing membranes (MVM) and fetal-facing plasma membranes (BM) from a term placenta.
  
  
  METHODS
  The component of buffer was improved based on Illsley method. The time of Mg2+ -aggregated basal membranes was extended. MVM were obtained from the supernatant of low speed centrifugation while BM were further purified on a sucrose step gradient.
  
  
  RESULTS
  Yield for MVM and BM prepared by the method were (0.55 +/- 10.10) mg/g and (0.54 +/- 0.02) mg/g wet weight of placenta. They were enriched 16.87-fold and 11.19-fold as determined by the membrane marker enzymes, alkaline phosphatase (MVM) and adenylate cyclase (BM).
  
  
  CONCLUSIONS
  The modified Illsley method can easily produce both MVM and BM of satisfied quantity from human placenta. It could be applied as a cell molecular model of maternal-fetal exchange interface.
authors.affiliation: Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Long
authors.forename: Ai-yun
authors.initials: AY
authors.lastname: Xing
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Yang
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D001485:Basement Membrane / Q000166:cytology*; D005260:Female; D006801:Humans; D008431:Maternal-Fetal Exchange / Q000502:physiology; D008871:Microvilli / Q000648:ultrastructure*; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D013048:Specimen Handling / Q000379:methods*
chemical_list: D000469:Alkaline Phosphatase