Does incubation temperature fluctuation influence hatchling phenotypes in reptiles? A test using parthenogenetic geckos.
Many lineages of parthenogenetic organisms have persisted through significant environmental change despite the constraints imposed by their fixed genotype and limited evolutionary potential. The ability of parthenogens to occur sympatrically with sexual relatives may in part be due to phenotypic plasticity in their responses to their environment, especially with respect to incubation temperature--a maternally selected trait. Here we measured the incubation temperatures selected by two lineages of triploid parthenogenic geckos in the Heteronotia binoei complex by allowing them to deposit clutches along a thermal gradient. The average nest temperature selected was 28.4 degrees C, with no significant differences between parthenogenic races or individual clones. To investigate the effect of nest-temperature variability on physiological and morphological traits, we incubated eggs from different races at one of four incubation regimes (32 degrees +/- 0 degrees, +/- 3 degrees , +/- 5 degrees , or +/- 9 degrees C). Embryos incubated at constant 32 degrees C developed faster than embryos reared under increasing extremes of diel temperature fluctuation (+/- 3 degrees , +/- 5 degrees C), and incubation at 32 degrees +/- 9 degrees C was unsuccessful. Incubation regime had no effect on the body size, preferred substrate temperature, or mass-specific .V(O2) of hatchlings. However, parthenogenic race had a significant effect on egg mass, tail length, snout-to-vent length, total length, and .V(O2) . We conclude that developmental traits are strongly influenced by clonal genotypes in this parthenogenic complex but are well buffered against fluctuations in incubation temperature.
Andrewartha
Sarah J
SJ
Adaptational and Respiratory Physiology Laboratory, Department of Zoology, La Trobe University, Melbourne, Victoria 3086, Australia.
Mitchell
Nicola J
NJ
Frappell
Peter B
PB
D000818:Animals; D004625:Embryo, Nonmammalian / Q000502:physiology; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D008116:Lizards / Q000033:anatomy & histology / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology; D010312:Parthenogenesis / Q000502:physiology*; D010641:Phenotype; D013696:Temperature