Magnetically recyclable, antimicrobial, and catalytically enhanced polymer-assisted "green" nanosystem-immobilized Aspergillus niger amyloglucosidase.
The present work reports the integration of polymer matrix-supported nanomaterial and enzyme biotechnology for development of industrially feasible biocatalysts. Aqueous leaf extract of Mesua ferrea L. was used to prepare silver nanoparticles distributed within a narrow size range (1-12 nm). In situ oxidative technique was used to obtain poly(ethylene glycol)-supported iron oxide nanoparticles (3-5 nm). Sonication-mediated mixing of above nanoparticles generated the immobilization system comprising of polymer-supported silver-iron oxide nanoparticles (20-30 nm). A commercially important enzyme, Aspergillus niger amyloglucosidase was coupled onto the immobilization system through sonication. The immobilization enzyme registered a multi-fold increment in the specific activity (807 U/mg) over the free counterpart (69 U/mg). Considerable initial activity of the immobilized enzyme was retained even after storing the system at room temperature as well as post-repeated magnetic recycling. Evaluation of the commendable starch saccharification rate, washing performance synergy with a panel of commercial detergents, and antibacterial potency strongly forwards the immobilized enzyme as a multi-functional industrially feasible system.
Konwarh
Rocktotpal
R
Department of Chemical Sciences, Tezpur University, Napaam, Assam, India.
Kalita
Dipankar
D
Mahanta
Charulata
C
Mandal
Manabendra
M
Karak
Niranjan
N
D001234:Aspergillus niger / Q000737:chemistry / Q000201:enzymology*; D001419:Bacteria / Q000187:drug effects; D055162:Biocatalysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry*; D055772:Green Chemistry Technology / Q000295:instrumentation / Q000379:methods*; D007700:Kinetics; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000382:microbiology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*
D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011108:Polymers; D005087:Glucan 1,4-alpha-Glucosidase