The correlation of 3D DT-MRI fiber disruption with structural and mechanical degeneration in porcine myocardium.
Evaluation of structural parameters following a myocardial infarction (MI) is important to assess left ventricular function and remodeling. In this study, we assessed the capability of 3D diffusion tensor magnetic resonance imaging (DT-MRI) to assess tissue degeneration shortly after an MI using a porcine model of infarction. Two days after an induced infarction, hearts were explanted and immediately scanned by a 3T MRI scanner with a diffusion tensor imaging protocol. 3D fiber tracks and clustering models were generated from the diffusion-weighted imaging data. We found in a normal explanted heart that DT-MRI fibers showed a multilayered helical structure, with fiber architecture and fiber density reflecting the integrity of muscle fibers. For infarcted heart explants, we observed either a lack of fibers or disruption of fibers in the infarcted regions. Contours of the disrupted DT-MRI fibers were found to be consistent with the infarcted regions. Both histological and mechanical analysis of the infarcted hearts suggested DT-MRI fiber disruption correlated with altered microstructure and tissue mechanics. The ability of 3D DT-MRI to accurately distinguish viable myocardium from dead myocardium only 2 days post infarct without the use of radioisotopes or ionotropic agents makes it a promising approach to evaluate cardiac damage early post-MI.
Zhang
Song
S
Department of Computer Science and Engineering, Mississippi State University, Mississippi State, MS, USA.
Crow
J Allen
JA
Yang
Xiaoyong
X
Chen
Joseph
J
Borazjani
Ali
A
Mullins
Katie B
KB
Chen
Wei
W
Cooper
Robert C
RC
McLaughlin
Ronald M
RM
Liao
Jun
J
D000818:Animals; D056324:Diffusion Tensor Imaging / Q000379:methods*; D004195:Disease Models, Animal; D006321:Heart / Q000000981:diagnostic imaging*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D009203:Myocardial Infarction / Q000000981:diagnostic imaging*; D009206:Myocardium; D011859:Radiography; D013552:Swine