Simultaneous determination of cefixime trihydrate and dicloxacillin sodium in pharmaceutical dosage form by reversed-phase high-performance liquid chromatography.
A simple, precise, accurate, and sensitive RP-HPLC method for simultaneous determination of cefixime trihydrate and dicloxacillin sodium in combined tablet dosage form was developed and validated. Chromatographic separation of the two drugs was performed on a Purospher BDS C18 column (25 cm x 4.6 mm id, 5 microm particle size). The mobile phase methanol-0.01 M phosphate buffer (75 + 25, v/v), adjusted to pH 3 with glacial acetic acid, was delivered at a flow rate of 1.0 mL/min. Detection was performed at 227 nm. Separation was completed within 10 min. Calibration curves were linear with R2 between 0.99 to 1.0 over a concentration range of 2-10 microg/mL for cefixime trihydrate and 5-25 micromL for dicloxacillin sodium. The RSD for intraday and interday precision was < 2.0%.
Dhoka
Madhura V
MV
Pune University, A.I.S.S.M.S. College of Pharmacy, Department of Quality Assurance, Pune-01, India. madhura1777@yahoo.com
Sandage
Shakuntala J
SJ
Dumbre
Snehal C
SC
D002021:Buffers; D002138:Calibration; D020682:Cefixime / Q000032:analysis*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004009:Dicloxacillin / Q000032:analysis*; D004304:Dosage Forms; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D010316:Particle Size; D010710:Phosphates / Q000737:chemistry; D015203:Reproducibility of Results; D013607:Tablets; D013678:Technology, Pharmaceutical / Q000379:methods*
D002021:Buffers; D004304:Dosage Forms; D010710:Phosphates; D013607:Tablets; D020682:Cefixime; D004009:Dicloxacillin