Aluminum stress increases carbon-centered radicals in soybean roots.
The formation of radical species was examined in roots of soybean seedlings exposed to aluminum (Al). Electron spin resonance (ESR) spectra of root homogenates with the spin-trapping reagent 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO) indicated the presence of carbon-centered radicals in plants not exposed to Al. Plants exposed to 50 microM Al showed a similar spectrum, with increased signal intensity. These radicals were likely produced through a H-atom abstraction reaction by hydroxyl (*OH) radicals, the synthesis of which was initiated by the formation of superoxide (O2*-) anions. The increased production of the carbon-centered radicals may be responsible for the lipid peroxidation in Al-treated roots.
Abo
Mitsuru
M
Department of Applied Biological Chemistry, School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
Yonehara
Hiroki
H
Yoshimura
Etsuro
E
D000535:Aluminum / Q000633:toxicity*; D002244:Carbon / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism*; D011758:Pyrroles / Q000378:metabolism; D012394:Rosaniline Dyes / Q000378:metabolism; D013025:Glycine max / Q000187:drug effects / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects*
C510912:(diethoxyphosphoryl)-5-methylpyrroline N-oxide; D005609:Free Radicals; D011758:Pyrroles; D012394:Rosaniline Dyes; C476677:Schiff's reagent; D017665:Hydroxyl Radical; D002244:Carbon; D000535:Aluminum