title: A diagnostic method for pneumocystis carinii a causative agent of pneumonia in immunodeficient rats.
abstract: Immunodeficient animals are in demand in current biomedical research, and they contribute to medical progress. In Pneumocystis infections, a specific histological diagnostic tool may be immunochemistry (IC). However, it was recently reported that the antibody (3F6) was not suitable for detecting Pneumocystis in rats. We purchased another antibody [PNC007] from a commercial source for IC. We could detect positive signals at identical locations with IC and Toluidine blue O in lungs of infected rats. These results corresponded to the results obtained with PCR. We should study the relationship between unexpected positive signals seen in IC and trophic forms in lungs of infected rats. We could clinically diagnose pneumonia caused by Pneumocystis carinii with the diagnostic method we developed.
authors.affiliation: Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Yabuuchi
authors.forename: Masaru
authors.initials: M
authors.lastname: Tajima
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kotani
authors.forename: Katsumi
authors.initials: K
authors.lastname: Aihara
authors.forename: Aiko
authors.initials: A
authors.lastname: Kawasaki
authors.forename: Shiro
authors.initials: S
authors.lastname: Kaneko
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takagi
authors.forename: Sumiko
authors.initials: S
authors.lastname: Kawai
authors.forename: Kyoko
authors.initials: K
authors.lastname: Shioya
authors.forename: Akira
authors.initials: A
authors.lastname: Okamoto
authors.forename: Soichiro
authors.initials: S
authors.lastname: Kagiyama
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kurosawa
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D005260:Female; D016867:Immunocompromised Host; D007150:Immunohistochemistry / Q000379:methods*; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D045363:Pneumocystis carinii / Q000276:immunology / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D051381:Rats; D011916:Rats, Inbred F344; D017208:Rats, Wistar
chemical_list: D000908:Antibodies, Fungal