Fiber-optic singlet oxygen [1O2 (1Delta(g))] generator device serving as a point selective sterilizer.
Traditionally, Type II heterogeneous photo-oxidations produce singlet oxygen via external irradiation of a sensitizer and external supply of ground-state oxygen. A potential improvement is reported here. A hollow-core fiber-optic device was developed with an "internal" supply of light and flowing oxygen, and a porous photosensitizer-end capped configuration. Singlet oxygen was delivered through the fiber tip. The singlet oxygen steady-state concentration in the immediate vicinity of the probe tip was ca 20 fm by N-benzoyl-DL-methionine trapping. The device is portable and the singlet oxygen-generating tip is maneuverable, which opened the door to simple disinfectant studies. Complete Escherichia coli inactivation was observed in 2 h when the singlet oxygen sensitizing probe tip was immersed in 0.1 mL aqueous samples of 0.1-4.4 x 10(7) cells. Photobleaching of the probe tip occurred after ca 12 h of use, requiring baking and sensitizer reloading steps for reuse.
Aebisher
David
D
Department of Chemistry, Graduate Center & The City University of New York, Brooklyn College, Brooklyn, NY, USA.
Zamadar
Matibur
M
Mahendran
Adaickapillai
A
Ghosh
Goutam
G
McEntee
Catherine
C
Greer
Alexander
A
D004926:Escherichia coli / Q000737:chemistry / Q000528:radiation effects; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D008027:Light; D010100:Oxygen / Q000737:chemistry; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000737:chemistry*; D026082:Singlet Oxygen / Q000737:chemistry*
D017319:Photosensitizing Agents; D026082:Singlet Oxygen; D010100:Oxygen