Dual-phase fermentation enables Actinobacillus succinogenes 130ZT to be a potential role for high-level lactate production from the bioresource.
Initial oxygen aeration enabled Actinobacillus succinogenes 130Z(T) to be regarded as a novel type of lactic-acid-producing strain in subsequent anaerobic cultivation. Lactic acid production increased 32-fold, up to the final titers of 135.6+/-0.14 g L(-1) with an overall yield of 0.96+/-0.09 g g(-1) glucose and 2.94+/-0.03 g L(-1) h(-1) productivity. The metabolites at the end of dual-phase fermentation by 130Z(T) were (in parentheses: the mol end product formed/100 mol glucose) succinic acid (37), acetic acid (69), formic acid (21), and lactic acid (193). Carbon flux distribution shifted toward to lactate in the bi-staged cultivation, where C(3) flux increased and C(4) flux reduced. The enzyme assay revealed that the lactate dehydrogenase (LDH) activity in dual-phase process was nearly 18-fold higher than the values in mono-phase process.
Li
Qiang
Q
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, PR China.
Wang
Dan
D
Song
Ziyu
Z
Zhou
Wei
W
Wu
Yong
Y
Xing
Jianmin
J
Su
Zhiguo
Z
D000188:Actinobacillus / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D005285:Fermentation; D019344:Lactic Acid / Q000096:biosynthesis*
D019344:Lactic Acid