[Expression and immunological characterization of the epitopes in tartary buckwheat allergen].
OBJECTIVE
To determine the most important region in tartary buckwheat allergen.


METHODS
The gene of epitopes was amplified by PCR using the primers designed according to TBa cDNA. Four expression vectors containing the gene of epitopes were constructed, and then transformed into the E.coli BL21(DE3) host cells. The expression products were purified by Ni(2+);-NTA agarose affinity chromatography column and indirect ELISA, inhibition ELISA and Dot blot was performed using sera from allergenic patients.


RESULTS
The purified proteins were obtained and the immunological results showed that E1 exhibite stronger IgE binding to patient's serum than the other epitopes.


CONCLUSIONS
E1 is probably the most important region in tartary buckwheat allergen binding to buckwheat allergic sera IgE.
Ren
Xiao-xia
XX
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.
Zhang
Xin
X
Cai
Gui-hong
GH
Li
Yu-ying
YY
Wang
Zhuan-hua
ZH
D000485:Allergens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D019612:Fagopyrum / Q000276:immunology*; D015151:Immunoblotting; D016133:Polymerase Chain Reaction
D000485:Allergens; D000939:Epitopes