title: Enzyme activity of catalase immobilized in Langmuir-Blodgett films of phospholipids.
abstract: A major challenge for producing low cost biosensors based on nanostructured films with control of molecular architectures is to preserve the catalytic activity of the immobilized biomolecules. In this study, we show that catalase (HRP) keeps its activity if immobilized in Langmuir-Blodgett (LB) films of dipalmitoyl phosphatidylglycerol (DPPG). The incorporation of catalase into a DPPG monolayer at the air-water interface was demonstrated with surface pressure and surface potential isotherms, in addition to polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). According to the PM-IRRAS data, catalase was not denatured upon adsorption on a preformed DPPG monolayer and could be transferred onto a solid substrate. The catalytic activity of catalase in a mixed LB film with DPPG was ca. 13% higher than in solution. The control of molecular architecture and choice of a suitable phospholipid matrix allows catalase-containing LB films to be used in sensing hydrogen peroxide.
authors.affiliation: Laboratório de Materiais Híbridos, Departamento de Ciências Exatas e da Terra, Universidade Federal de São Paulo, Diadema, SP, Brazil.
authors.forename: Thiago E
authors.initials: TE
authors.lastname: Goto
authors.forename: Ricardo F
authors.initials: RF
authors.lastname: Lopez
authors.forename: Osvaldo N
authors.initials: ON
authors.lastname: Oliveira
authors.forename: Luciano
authors.initials: L
authors.lastname: Caseli
mesh_terms: D015374:Biosensing Techniques; D002374:Catalase / Q000737:chemistry* / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D008567:Membranes, Artificial; D008962:Models, Theoretical; D010715:Phosphatidylglycerols / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D010715:Phosphatidylglycerols; D010743:Phospholipids; D002374:Catalase; C030345:1,2-dipalmitoylphosphatidylglycerol