title: [Evaluation of morphological changes of the liver caused by heroin abuse in forensic practice].
abstract: OBJECTIVE
  A study of morphological lesions in the liver of heroin addicts enables a precise overview of the type and degree of the liver damages caused by intravenous (i.v.) heroin abuse, additive effects of viral infections and alcohol consumption, as well as whether the expressiveness of these lesions depends on the duration of the time period of heroin application. The aim of the study was to investigate histopathological, ultrastructural and morphometric features of the liver of heroin addicts in forensic samples of the liver.
  
  
  METHODS
  The study involved the autopsy conducted on 40 bodies of i.v. heroin addicts and 10 control autopsies. The investigated group consisted of liver samples of 36 male subjects and 4 female subjects aged 35-40 years and the control group of 8 male and 2 female cadaveric bodies aged 15-35 years. The liver tissue samples were prepared for light microscopy. Sections of the tissue paraffin blocks 5 micro thick were stained using classical Hematoxylin and Eosin method (H&E), as well as PAS Van Gieson, Gomori, and Congo Red techniques. For investigation purposes of ultrastructural changes, liver tissue was fixed in glutaraldehyde and molded with epon. The analysis was performed using the method of transmission electron microscopy. Morphometric investigation of the liver sinusoidal macrophages was performed by using the M42 test system.
  
  
  RESULTS
  In the investigated group of i.v. heroin addicts, the liver autopsy samples showed degenerative vesicular and fat changes, chronic hepatitis, cirrhosis, sedimentation of pathologic protein amyloidosis, dysplastic changes, reduction in the amount of glycogen in hepatocytes, as well as the change in the number of Kupfer and endothelial cells. The established changes correlated with the duration of i.v. heroin abuse, whereas sinusoidal macrophages were activated in cases with active hepatitis, and no significant change in their number was found in hepatocytes with alcohol-related fatty changes. CONCLUSION. The study showed that the most present change in the hepatocytes of drug addicts was vesicular degeneration, and it is the only direct consequence of the effect of heroin. Other morphological changes were present due to viral infections and they correlated with the duration of narcotic abuse. The finding of dysplastic changes in this susceptible population of young people is particularly significant. The forensic significance of the established changes in the liver tissue is in the possibility of their practical application for determination of the immediate cause of death of i.v. heroin addicts, as well as the differential diagnosis of not only heroin, but also alcohol, sedative and other substances abuse, and all that on the basis of morphological damages of the liver.
authors.affiliation: Medicinski fakultet, Zavod za sudsku medicinu, Nis, Srbija.
authors.forename: Goran
authors.initials: G
authors.lastname: Ilić
authors.forename: Jasmina
authors.initials: J
authors.lastname: Gligorijević
authors.forename: Ivica
authors.initials: I
authors.lastname: Milosavljević
authors.forename: Radovan
authors.initials: R
authors.lastname: Karadzić
mesh_terms: D000293:Adolescent; D000328:Adult; D001344:Autopsy; D005260:Female; D049429:Forensic Pathology; D006556:Heroin Dependence / Q000473:pathology*; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000473:pathology*; D008297:Male; D011180:Postmortem Changes; D055815:Young Adult