The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard.
The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.
Peng
Lifeng
L
Centre for Biodiscovery and School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
Kapp
Eugene A
EA
Fenyö
David
D
Kwon
Min-Seok
MS
Jiang
Pu
P
Wu
Songfeng
S
Jiang
Ying
Y
Aguilar
Marie-Isabel
MI
Ahmed
Nikhat
N
Baker
Mark S
MS
Cai
Zongwei
Z
Chen
Yu-Ju
YJ
Van Chi
Phan
P
Chung
Maxey C M
MC
He
Fuchu
F
Len
Alice C L
AC
Liao
Pao-Chi
PC
Nakamura
Kazuyuki
K
Ngai
Sai Ming
SM
Paik
Young-Ki
YK
Pan
Tai-Long
TL
Poon
Terence C W
TC
Salekdeh
Ghasem Hosseini
GH
Simpson
Richard J
RJ
Sirdeshmukh
Ravi
R
Srisomsap
Chantragan
C
Svasti
Jisnuson
J
Tyan
Yu-Chang
YC
Dreyer
Florian S
FS
McLauchlan
Danyl
D
Rawson
Pisana
P
Jordan
T William
TW
D000818:Animals; D001208:Asia; D002254:Carbonates; D002462:Cell Membrane / Q000737:chemistry*; D006801:Humans; D007425:Intracellular Membranes / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry* / Q000592:standards; D051379:Mice; D044349:Oceania; D009938:Organizations; D020543:Proteome; D040901:Proteomics / Q000379:methods / Q000592:standards*
D002254:Carbonates; D008565:Membrane Proteins; D020543:Proteome