Effects of irrigation solutions on dentin wettability and roughness.
BACKGROUND
Irrigation solutions are used to disinfect the root canal, flush out debris, and dissolve pulp tissue. It is hypothesized that irrigation solutions might influence the physicochemical properties of human root canal dentin including the wettability and roughness.


METHODS
The roots of 20 human permanent anterior teeth were sectioned longitudinally into dentin slices and randomly divided into 4 groups with different treatments as follows: (1) 17% ethylenediaminetetraacetic acid (EDTA) for 10 minutes, (2) 5.25% NaOCl for 10 minutes, (3) 3% H2O2 for 10 minutes, and (4) distilled water as control. Wettability was evaluated by contact angle measurement. Atomic force microscopy was used to measure the surface roughness (Ra).


RESULTS
Water contact angles significantly decreased from 54.35 degrees (control) to 22.04 and 46.35 degrees on the dentin treated by 5.25% NaOCl and 3% H2O2, respectively, with the wettability increasing accordingly (both P < .05). The roughness of dentin surfaces (Ra) was increased from 85.39 (control) to 121.90 and 140.49 nm by 5.25% NaOCl and 17% EDTA, respectively (both P < .05).


CONCLUSIONS
These findings indicated that all the irrigation solutions could alter the physicochemical characteristics of dentin surfaces and might influence bacterial adhesion.
Hu
Xiaoli
X
Department of Operative Dentistry and Endodontics, Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou, China.
Ling
Junqi
J
Gao
Yan
Y
D000328:Adult; D002614:Chelating Agents / Q000494:pharmacology; D055598:Chemical Phenomena; D003786:Dental Pulp Cavity / Q000187:drug effects* / Q000648:ultrastructure; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008875:Middle Aged; D016877:Oxidants / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013499:Surface Properties; D014867:Water; D017155:Wettability
D002614:Chelating Agents; D016877:Oxidants; D012388:Root Canal Irrigants; D014867:Water; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite