Comparative analysis of detoxification enzymes in Acyrthosiphon pisum and Myzus persicae.
Herbivorous insects use detoxification enzymes, including cytochrome P450 monooxygenases, glutathione S-transferases, and carboxy/cholinesterases, to metabolize otherwise deleterious plant secondary metabolites. Whereas Acyrthosiphon pisum (pea aphid) feeds almost exclusively from the Fabaceae, Myzus persicae (green peach aphid) feeds from hundreds of species in more than forty plant families. Therefore, M. persicae as a species would be exposed to a greater diversity of plant secondary metabolites than A. pisum, and has been predicted to require a larger complement of detoxification enzymes. A comparison of M. persicae cDNA and A. pisum genomic sequences is partially consistent with this hypothesis. There is evidence of at least 40% more cytochrome P450 genes in M. persicae than in A. pisum. In contrast, no major differences were found between the two species in the numbers of glutathione S-transferases, and carboxy/cholinesterases. However, given the incomplete M. persicae cDNA data set, the number of identified detoxification genes in this species is likely to be an underestimate.
Ramsey
J S
JS
Boyce Thompson Institute for Plant Research, Ithaca, NY, USA.
Rider
D S
DS
Walsh
T K
TK
De Vos
M
M
Gordon
K H J
KH
Ponnala
L
L
Macmil
S L
SL
Roe
B A
BA
Jander
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D001711:Biotransformation / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism; D002802:Cholinesterases / Q000235:genetics / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D049750:Genome, Insect; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000378:metabolism / Q000469:parasitology; D010802:Phylogeny; D027861:Prunus / Q000378:metabolism / Q000469:parasitology; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D017931:DNA Primers; D018076:DNA, Complementary; D019476:Insect Proteins; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase; D002802:Cholinesterases