GABA and glutamate fluxes in developing nerve endings.
Radiolabeled GABA and glutamate transport into 7 day, 14 day and adult cortical nerve ending preparations was examined. Transport was measured at several Na(+) concentrations, 19, 27, 43 and 121 mM, and at two temperatures, 15 and 30 degrees C. K(m) and V(max) values were calculated for all experimental conditions by means of Wilkinson (1961) analysis. A comparison of the day 14 and adult data shows higher K(m) values at all Na(+) concentrations on day 14 for both GABA and glutamate transport. In addition, the temperature dependence of transport was attenuated in the day 14 preparation. Finally, the specificity of GABA transport, as measured by the use of the transport inhibitors ?-alanine and 2,4-diaminobutyric acid, was not different between the day 14 and adult preparations. Overall, it is concluded that both GABA and glutamate transport into day 14 nerve endings behave as if "adult" transporter molecules were existing in a more fluid lipid environment, which is the situation found in synaptic membranes prepared from day 14 nerve endings (Hitzemann and Johnson, 1983). Glutamate and GABA transport into 7 day nerve endings is complex and shows marked differences from the day 14 and adult data. Day 7 GABA transport was significantly more sensitive to ?-alanine inhibition. Day 7 transport was more sensitive to Na(+) manipulation and the temperature dependent kinetics show unique Na(+) effects not seen in the day 14 or adult preparations. For example, at 19 mM Na(+), 7 day glutamate transport was more temperature dependent than adult transport but as the Na(+) concentration was increased the reverse was true. The opposite situation for temperature-Na(+) effects was seen for GABA transport. Finally, no Ca(+2)-dependent component of GABA release could be found in 7 day nerve endings while a significant component was found at day 14. Overall, it is concluded that both glutamate and GABA fluxes in 7 day nerve endings differ both qualitatively from that seen in both day 14 and adult nerve endings.
Hitzemann
R
R
Laboratory of Psychobiology, Departments of Psychiatry and Pharmacology and Cell Biophysics, University of Cincinnati, Cincinnati, Ohio 45267, U.S.A.
Mark
C
C
Panini
A
A