Innate immune parameters and haemolymph protein expression profile to evaluate the immunotoxicity of tributyltin on abalone (Haliotis diversicolor supertexta).
The immunotoxicity of tributyltin (TBT) on marine gastropods has been comparatively little studied although risks to wildlife associated with this compound are well known. In this study, a 30-day trial was conducted to evaluate the immunotoxic effects on abalone (Haliotis diversicolor supertexta) by exposing a range of doses of TBT (0, 2, 10, and 50 ng/L). Innate immune parameters, including phagocytic ability (PA), lysozyme activity, phenoloxidase (PO) level and superoxide dismutase (SOD) activity were monitored at intervals of 5, 15 and 30 days. Haemolymph protein expression profile was also examined at the end of the experiment. The results showed that PA value, lysozyme activity and PO level significantly decreased compared with the controls (P < 0.05), which indicated that TBT exposure markedly suppressed non-specific immune competence. Exposure to TBT also caused variation in protein expression patterns of haemolymph. Among the protein spots of differential expressions, seven proteins from the haemolymph of TBT-treated abalone were successfully identified by MALDI-TOF-MS analysis. Three protein spots increased and were identified as carrier-like peptide, peroxidase 21 precursor and creatine phosphokinase. These proteins are believed to up-regulate in expression as a response to detoxification and antioxidative stress mechanisms. The other four protein spots that down-regulated in TBT-treated groups were identified as aromatase-like protein, protein kinase C, ceruloplasmin and microtubule-actin crosslinking factor 1, and these proteins play an important role in endocrine regulation and immune defense. Taken together, the results demonstrate that TBT impair abalone immunological ability and is a potential immune disruptor.
Zhou
Jin
J
Life Sciences Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, PR China.
Cai
Zhong-Hua
ZH
Zhu
Xiao-Shan
XS
Li
Lei
L
Gao
Yun-Feng
YF
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004202:Disinfectants / Q000008:administration & dosage* / Q000009:adverse effects; D004781:Environmental Exposure / Q000009:adverse effects; D049851:Gastropoda; D020869:Gene Expression Profiling; D006458:Hemolymph / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007113:Immunity, Innate / Q000187:drug effects; D007165:Immunosuppression Therapy; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D010586:Phagocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D010587:Phagocytosis / Q000187:drug effects; D014220:Trialkyltin Compounds / Q000008:administration & dosage* / Q000009:adverse effects
D000975:Antioxidants; D004202:Disinfectants; C095003:MACF1 protein, human; D008840:Microfilament Proteins; D014220:Trialkyltin Compounds; C011559:tributyltin; D009195:Peroxidase; D001141:Aromatase