Hybrid-scanning optical-resolution photoacoustic microscopy for in vivo vasculature imaging.
Recently developed optical-resolution photoacoustic microscopy (OR-PAM), which is based on the detection of optical absorption contrast, is complementary to other optical microscopy modalities such as optical confocal microscopy, optical coherence tomography, and multiphoton microscopy. A hybrid optical-mechanical scanning configuration increases the imaging speed of OR-PAM significantly, facilitating many demanding biomedical applications. With a high-pulse-repetition-rate laser, the hybrid-scanning OR-PAM can acquire one-dimensional depth-resolved images (A-lines) at 5 kHz and two-dimensional B-scan images containing 800 A-lines at 6.25 Hz. We demonstrated in vivo in a mouse three-dimensional imaging of the iris vasculature in 128 s for an 800x800x200 data set and of the ear vasculature in 256 s for an 800x1600x200 data set.
Rao
Bin
B
Department of Biomedical Engineering, Optical Imaging Lab, Washington University in St. Louis, Campus Box 1097,One Brookings Drive, St. Louis, Missouri 63130-4899, USA.
Li
Li
L
Maslov
Konstantin
K
Wang
Lihong
L
D000818:Animals; D004423:Ear / Q000098:blood supply; D007498:Iris / Q000098:blood supply; D051379:Mice; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D055620:Optical Phenomena