β-Adrenoceptor-mediated differences in transverse and longitudinal strips from the rat detrusor.
Contractions and relaxations of the urinary bladder occur in all directions to facilitate urine release and storage. Transverse and longitudinal contractility of detrusor smooth muscle have been studied before using various pharmacologic stimuli but not β agonists. Given the importance of β-adrenoceptors in mediating bladder relaxation, the effects of isoprenaline (IPNA) in transverse and longitudinal contractility were examined. Pretreatment with a low concentration of IPNA (0.1 or 1 μM) suppressed carbachol (CCh)-induced contractions, more in the transverse than longitudinal direction. Increasing the IPNA concentration to 10 or 100 μM resulted in greater inhibition of longitudinal contractions. Also in the longitudinal direction, IPNA-induced relaxation was greater than in the transverse direction. When precontracted with a submaximal concentration of CCh (1 μM), IPNA increased the phasic activity in the longitudinal direction only. In summary, β-adrenoceptor-mediated differences between transverse and longitudinal contractility were revealed. In testing the relaxant properties of selective β-agonists, the findings here should be considered such that other than the conventional longitudinal contractions, measurements are also made in other directions.
Liang
Willmann
W
School of Biological Sciences, Nanyang Technological University, Singapore, 637551, Singapore. willmann@ntu.edu.sg
Lo
Wan Ning
WN
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D005260:Female; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009126:Muscle Relaxation / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism*; D001743:Urinary Bladder / Q000187:drug effects / Q000502:physiology*
D000318:Adrenergic beta-Agonists; D011943:Receptors, Adrenergic, beta; D007545:Isoproterenol