title: Human tissue distribution of paraoxonases 1 and 2 mRNA.
abstract: We have studied the distribution of mRNA for paraoxonases (PON) 1 and 2 in 24 human tissues using Gene Expression Panels. PON1 mRNA was restricted to adult kidney, liver, and colon as well as fetal liver, whereas PON2 mRNA was more widely distributed in adult human brain, heart, kidney, spleen, liver, colon, lung, small intestine, muscle, stomach, testis, placenta, salivary, thyroid and adrenal glands, pancreas, skin, and bone marrow, as well as fetal brain and liver. PON2 mRNA was not found in ovary, uterus, or plasma leukocytes using the panels. However, using real time PCR, we found PON2 mRNA expression in human plasma leukocytes. There were differences between the tissue distribution of mRNAs found in this study and the immunohistochemical localization of the PON1 and PON2 proteins reported previously. In particular, PON1 protein is much more widely distributed than its mRNA, possibly indicating the delivery of PON1 to various tissues by HDL. In addition, differences between PON2 mRNA and protein distributions could be due to missence mutations in the PON2 gene, causing nontranslation of mRNA to protein in some tissues.
authors.affiliation: Centre de Recerca Biomedica, Hospital Universitari de Sant Joan, C. Sant Joan S/N, Reus, Catalunya, Spain. mike.mackness@gmail.com
authors.forename: Bharti
authors.initials: B
authors.lastname: Mackness
authors.forename: Raul
authors.initials: R
authors.lastname: Beltran-Debon
authors.forename: Gerard
authors.initials: G
authors.lastname: Aragones
authors.forename: Jorge
authors.initials: J
authors.lastname: Joven
authors.forename: Jordi
authors.initials: J
authors.lastname: Camps
authors.forename: Mike
authors.initials: M
authors.lastname: Mackness
mesh_terms: D043303:Aryldialkylphosphatase / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D043303:Aryldialkylphosphatase