title: Expression of the T-cell-specific adapter protein in oral epithelium.
abstract: The multifunctional T-cell-specific adapter protein (TSAd) was originally described in T cells but is also expressed in epithelial cells from the respiratory tract and in endothelium. In this study, we found expression of TSAd messenger RNA (mRNA) and protein in both human and murine oral mucosal epithelium as well as in human primary oral keratinocyte cell cultures. In TSAd(-/-) mice, the mucosa and skin appeared macroscopically normal, but severe disturbances were observed in the fine structures of the basal membrane and intercellular epithelial spaces upon analysis using transmission electron microscopy. Oral epithelial cells from TSAd(-/-) mice displayed decreased migration compared with cells from wild-type mice, whereas overexpression of TSAd in a human epithelial cell line resulted in impaired proliferation. This study is the first to show that TSAd is expressed in normal oral mucosa, that it is important for the normal ultrastructural morphology of the epithelium and the basal membrane, and that it is involved in the migration and proliferation of oral keratinocytes.
authors.affiliation: Department of Oral Biology, Faculty of Dentistry, University of Oslo, Norway. k.m.kolltveit@odont.uio.no
authors.forename: Kristin M
authors.initials: KM
authors.lastname: Kolltveit
authors.forename: Olav
authors.initials: O
authors.lastname: Schreurs
authors.forename: Janet
authors.initials: J
authors.lastname: Ostrem
authors.forename: Tine M
authors.initials: TM
authors.lastname: Søland
authors.forename: Cuong
authors.initials: C
authors.lastname: Khuu
authors.forename: Tone
authors.initials: T
authors.lastname: Berge
authors.forename: Edward
authors.initials: E
authors.lastname: Messelt
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Hayashi
authors.forename: Stine
authors.initials: S
authors.lastname: Granum
authors.forename: Anne
authors.initials: A
authors.lastname: Spurkland
authors.forename: Karl
authors.initials: K
authors.lastname: Schenck
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis* / Q000235:genetics; D000818:Animals; D001485:Basement Membrane / Q000166:cytology / Q000648:ultrastructure; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000166:cytology / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000166:cytology / Q000648:ultrastructure; D007633:Keratins / Q000032:analysis; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D046529:Microscopy, Electron, Transmission; D009061:Mouth Mucosa / Q000166:cytology* / Q000648:ultrastructure; D014162:Transfection
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D008562:Membrane Glycoproteins; C402726:Sh2d2a protein, mouse; C029967:nidogen; D007633:Keratins