title: Enantioselective separation of the carfentrazone-ethyl enantiomers in soil, water and wheat by HPLC.
abstract: A simple enantioselective HPLC method was developed for measuring carfentrazone-ethyl enantiomers. The separation and determination was accomplished on an amylose tris[(S)-alpha-methylbenzylcarbamate] (Chiralpak AS) column using n-hexane/ethanol (98:2, v/v) as mobile phase at a flow rate of 1.0 mL/min with UV detection at 248 nm. The effects of mobile-phase composition and column temperature on the enantioseparation were discussed. The accuracy, precision, linearity, LODs, and LOQ of the method were also investigated. LOD was 0.001 mg/kg in water, 0.015 mg/kg in soil and wheat, with an LOQ of 0.0025 mg/kg in water and 0.05 mg/kg in soil and wheat for each enantiomer of carfentrazone-ethyl. SPE was used for the enrichment and cleanup of soil, water, and wheat samples. Recoveries for two enantiomers were 88.4-106.7% with RSD(r) of 4.2-9.8% at 0.1, 0.5, and 1 mg/kg levels from soil, 85.8-99.5% with the RSD(r) of 4.4-9.6% at 0.005, 0.025, and 0.05 mg/kg levels from water, and from wheat the recoveries were 86.3-91.3% with RSD(r) below 5.0% at 0.2, 0.5, and 1 mg/kg levels. This method could be used to identify and quantify the carfentrazone-ethyl enantiomers in food and environment.
authors.affiliation: Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Key Laboratory of Pesticide Chemistry and Application, Ministry of Agriculture, Beijing, P. R. China. lyb041011048@163.com
authors.forename: Yuanbo
authors.initials: Y
authors.lastname: Li
authors.forename: Fengshou
authors.initials: F
authors.lastname: Dong
authors.forename: Xingang
authors.initials: X
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Wuying
authors.initials: W
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Cheng
authors.forename: Ping
authors.initials: P
authors.lastname: Ning
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yunhao
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D012989:Soil Pollutants / Q000737:chemistry*; D013237:Stereoisomerism; D014230:Triazoles / Q000737:chemistry / Q000302:isolation & purification*; D014908:Triticum / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D012989:Soil Pollutants; D014230:Triazoles; D014867:Water; C427526:carfentrazone-ethyl