Searching for mesophilic Thermotogales bacteria: "mesotogas" in the wild.
All cultivated Thermotogales are thermophiles or hyperthermophiles. However, optimized 16S rRNA primers successfully amplified Thermotogales sequences from temperate hydrocarbon-impacted sites, mesothermic oil reservoirs, and enrichment cultures incubated at <46 degrees C. We conclude that distinct Thermotogales lineages commonly inhabit low-temperature environments but may be underreported, likely due to "universal" 16S rRNA gene primer bias.
Nesbø
Camilla L
CL
Department of Biology, Blindern, 0316 Oslo, Norway. camillaln@gmail.com
Kumaraswamy
Rajkumari
R
Dlutek
Marlena
M
Doolittle
W Ford
WF
Foght
Julia
J
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D004785:Environmental Pollutants / Q000032:analysis; D006838:Hydrocarbons / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004785:Environmental Pollutants; D006838:Hydrocarbons; D012336:RNA, Ribosomal, 16S