[ComE-deficient mutant affects in vivo-induced genes expression in Streptococcus pneumoniae].
OBJECTIVE
To screen in vivo genes of Streptococcus pneumoniae controlled by comE gene.


METHODS
The comE-deficient strain was constructed by using insertion inactivation and identified by PCR and sequencing. The BALB/c mouse was used as test animal and injected with D39 wild type and D39 comE-deficient strain via intraperitoneal injection. The mice blood was obtained about 24 hours after the injection through posterior orbital venous plexus approach. Then the bacteria induced in vivo were collected from the blood and their RNA were extracted to measure the mRNA expression levels of each in vivo-induced gene by RT-PCR.


RESULTS
The differences of the expressions of 8 in vivo-induced genes in D39 and D39 comE-deficient strain were statistically significant (P < 0.05) and in which spd_0300, spd_0414, spd_ 0622, spd_1663, spd_1719, spd_0235, spd_0873 were up-regulated by transformation and meanwhile spd_1672 was down-regulated.


CONCLUSIONS
In vivo-induced genes spd_0300, spd_0414, spd_0622, spd_1663, spd_1719, spd 0235, spd_0873, spd_1672 regulated by transformation were screened out and they may participate in the processes such as growth regulation, temperature reception, carbohydrate metabolism, and lipoids metabolism. The bacterial transformation may enhance the virulence via regulating some in vivo-induced genes expression.
Liu
Xin
X
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. zxkglx@126.com
Yin
Nanlin
N
Zhang
Xuemei
X
Yang
Xiaoliang
X
Pang
Dan
D
Wang
Hong
H
Yin
Yibing
Y
Xu
Wenchun
W
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D016254:Mutagenesis, Insertional; D011008:Pneumococcal Infections / Q000235:genetics / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013296:Streptococcus pneumoniae / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics / Q000502:physiology
D001426:Bacterial Proteins; C103179:ComE protein, Streptococcus pneumoniae