Analysis of the association between lactotransferrin (LTF) gene polymorphism and dental caries.
OBJECTIVE
The present study evaluated the association between lactotransferrin (LTF) gene polymorphism (exon 2, A/G, Lys/Arg) and dental caries.


METHODS
A convenience sample of 110 individuals, 12 years old, was divided into: group 1, 48 individuals without caries experience (DMFT=0), and group 2, 62 subjects with caries experience (DMFT>or=1). DNA was obtained from a mouthwash with 3% glucose solution, followed by a scrapping of the oral mucosa. After DNA purification, polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP) was performed to access the study polymorphism. The LTF A/G (Lys/Arg) polymorphism had been previously reported as located in exon 1.


RESULTS
Allele 1 of the study polymorphism was associated with low DMFT index and showed a protective effect against caries experience (OR=0.16, IC=0.03-0.76, p=0.01).


CONCLUSIONS
Lactotransferrin A/G (exon 2, Lys/Arg) polymorphism was associated with susceptibility to dental caries in 12-year-old students.
Azevedo
Luiza Foltran
LF
Center for Health and Biological Sciences, Pontifical Catholic University of Paraná, Curitiba, PR, Brazil.
Pecharki
Giovana Daniela
GD
Brancher
João Armando
JA
Cordeiro
Carlos Alberto
CA
Medeiros
Kamilla Gabriella dos Santos
KG
Antunes
Alessandra Armstrong
AA
Arruda
Eduardo Silva
ES
Werneck
Renata Iani
RI
de Azevedo
Luciana Reis
LR
Mazur
Rui Fernando
RF
Moysés
Samuel Jorge
SJ
Moysés
Simone Tetü
ST
Faucz
Fábio Rueda
FR
Trevilatto
Paula Cristina
PC
D000483:Alleles; D019943:Amino Acid Substitution; D001120:Arginine; D001483:Base Sequence; D016022:Case-Control Studies; D002648:Child; D004245:DMF Index; D004252:DNA Mutational Analysis; D003731:Dental Caries / Q000235:genetics*; D003733:Dental Caries Susceptibility / Q000235:genetics*; D005787:Gene Frequency; D006801:Humans; D007781:Lactoferrin / Q000235:genetics*; D008239:Lysine; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D012463:Saliva / Q000378:metabolism
D001120:Arginine; D007781:Lactoferrin; D008239:Lysine