title: Acetoacetate, d-3-hydroxybutyrate and glucose utilization by capillaries isolated from developing rat brain.
abstract: Isolated cerebral capillaries from developing rats utilize glucose as well as ketone bodies essentially for oxidative metabolism. However, CO(2) production from [U-(14)C]glucose was significantly greater than from ketone bodies (except at 5 mM). Ketone body utilization (in the presence of 5 mM glucose in the incubation medium) was concentration-dependent (up to 5 mM). Lipid synthesis from ketone bodies was comparable to that from glucose up to 1 mM. At concentrations ? 1 mM, acetoacetate incorporation into total lipids and fatty acids was higher than other substrates, however, this difference was statistically significant only at 5 mM. Incorporation of substrates into sterols was very low (> 1 pmol/h/mg protein).
authors.affiliation: INSERM U.26, Hôpital F. Widal, 200 rue du Fg. St-Denis, 75475 Paris, Cedex 10, France.
authors.forename: P
authors.initials: P
authors.lastname: Homayoun
authors.forename: J M
authors.initials: JM
authors.lastname: Bourre