Auxin-related gene families in abiotic stress response in Sorghum bicolor.
Sorghum, a C4 model plant, has been studied to develop an understanding of the molecular mechanism of resistance to stress. The auxin-response genes, auxin/indole-3-acetic acid (Aux/IAA), auxin-response factor (ARF), Gretchen Hagen3 (GH3), small auxin-up RNAs, and lateral organ boundaries (LBD), are involved in growth/development and stress/defense responses in Arabidopsis and rice, but they have not been studied in sorghum. In the present paper, the chromosome distribution, gene duplication, promoters, intron/exon, and phylogenic relationships of Aux/IAA, ARF, GH3, and LBD genes in sorghum are presented. Furthermore, real-time PCR analysis demonstrated these genes are differently expressed in leaf/root of sorghum and indicated the expression profile of these gene families under IAA, brassinosteroid (BR), salt, and drought treatments. The SbGH3 and SbLBD genes, expressed in low level under natural condition, were highly induced by salt and drought stress consistent with their products being involved in both abiotic stresses. Three genes, SbIAA1, SbGH3-13, and SbLBD32, were highly induced under all the four treatments, IAA, BR, salt, and drought. The analysis provided new evidence for role of auxin in stress response, implied there are cross talk between auxin, BR and abiotic stress signaling pathways.
Wang
SuiKang
S
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Bai
YouHuang
Y
Shen
ChenJia
C
Wu
YunRong
Y
Zhang
SaiNa
S
Jiang
DeAn
D
Guilfoyle
Tom J
TJ
Chen
Ming
M
Qi
YanHua
Y
D000818:Animals; D002874:Chromosome Mapping; D017343:Genes, Plant; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D045868:Sorghum / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological / Q000235:genetics*
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins