Effects of desmopressin on platelet function under conditions of hypothermia and acidosis: an in vitro study using multiple electrode aggregometry*.
Hypothermia and acidosis lead to an impairment of coagulation. It has been demonstrated that desmopressin improves platelet function under hypothermia. We tested platelet function ex vivo during hypothermia and acidosis. Blood samples were taken from 12 healthy subjects and assigned as follows: normal pH, pH 7.2, and pH 7.0, each with and without incubation with desmopressin. Platelet aggregation was assessed by multiple electrode aggregometry. Baseline was normal pH and 36 degrees C. The other samples were incubated for 30 min and measured at 32 degrees C. Acidosis significantly impaired aggregation. Desmopressin significantly increased aggregability during hypothermia and acidosis regardless of pH, but did not return it to normal values at low pH. During acidosis and hypothermia, acidosis should be corrected first; desmopressin can then be administered to improve platelet function as a bridge until normothermia can be achieved.
Hanke
A A
AA
Department of Anaesthesiology and Intensive Care Medicine, Hannover Medical School, Hannover, Germany. asait@takii.kmu.ac.jp
Dellweg
C
C
Kienbaum
P
P
Weber
C F
CF
Görlinger
K
K
Rahe-Meyer
N
N
D000138:Acidosis / Q000097:blood*; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology*; D005260:Female; D006490:Hemostatics / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007035:Hypothermia / Q000097:blood*; D008297:Male; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D055815:Young Adult
D006490:Hemostatics; D000244:Adenosine Diphosphate; D003894:Deamino Arginine Vasopressin