Label-free biosensing by means of periodic lattices of high aspect ratio SU-8 nano-pillars.
We developed biophotonic sensing arrays of 60x60 microm(2) made of periodic lattices of high aspect ratio SU-8 nano-pillars in order to demonstrate their capability for label-free molecule detection, as well as the sensitivity enhancement in comparison with a single layer of SU-8. The biophotonic sensing arrays, that we call BICELLs (Biophotonic sensing cells), are interrogated vertically by using micron spot size Fourier transform visible and IR spectrometry (FT-VIS-IR). We monitored the surface immobilization of bovine serum albumin (BSA) antigen and anti-BSA antibody (aBSA) recognition. The bioassay exhibits a limit of detection (LOD) in the order of 2 ng/ml limited by the wavenumber uncertainty during the interrogation process. We also estimated and compared the theoretical biolayer thickness with previous results.
Holgado
M
M
Centro Láser UPM, Universidad Politécnica de Madrid, Madrid, Spain. m.holgado@upm.es
Barrios
C A
CA
Ortega
F J
FJ
Sanza
F J
FJ
Casquel
R
R
Laguna
M F
MF
Bañuls
M J
MJ
López-Romero
D
D
Puchades
R
R
Maquieira
A
A
D000818:Animals; D000906:Antibodies / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002417:Cattle; D004852:Epoxy Compounds; D004867:Equipment Design; D021621:Imaging, Three-Dimensional; D055767:Immobilized Proteins / Q000276:immunology; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D055620:Optical Phenomena; D011108:Polymers; D012710:Serum Albumin, Bovine / Q000276:immunology; D012822:Silicon Dioxide; D017550:Spectroscopy, Fourier Transform Infrared
D000906:Antibodies; D004852:Epoxy Compounds; D055767:Immobilized Proteins; D011108:Polymers; C520895:SU-8 compound; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide