A direct continuous fluorometric turn-on assay for monoamine oxidase B and its inhibitor-screening based on the abnormal fluorescent behavior of silole.
As essential enzymes in the body, monoamine oxidases (MAOs), including MAO-B, help maintain the proper balance of neurotransmitters and other dietary and biogenic amines. Thus, development of convenient and continuous assays for MAO-B and its inhibitor-screening is highly desirable since the inhibitors have implications for therapeutics for many brain diseases. In this paper, we report a direct continuous fluorescence turn-on assay for MAO-B and its inhibitor-screening with the ensemble of silole 1, heptylamine and HSO(3)(-), which are either easily accessible or commercially available. Fluorescent spectral investigations show that this fluorometric assay can be performed continuously in aqueous solution and MAO-B of concentrations as low as 0.25 microg/mL can be analyzed. Moreover, the ensemble of silole 1, heptylamine, HSO(3)(-) and MAO-B can be employed to screen the inhibitors of MAO-B.
Peng
Lihua
L
Beijing National Laboratory for Molecular Science, Organic Solids Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Zhang
Guanxin
G
Zhang
Deqing
D
Wang
Yilin
Y
Zhu
Daoben
D
D000588:Amines / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D008995:Monoamine Oxidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000737:chemistry*; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D012821:Silanes / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry
D000588:Amines; D005456:Fluorescent Dyes; D008996:Monoamine Oxidase Inhibitors; D011994:Recombinant Proteins; D012821:Silanes; D013464:Sulfuric Acids; C540376:silole; D008995:Monoamine Oxidase