Detection of all species of the genus Alphavirus by reverse transcription-PCR with diagnostic sensitivity.
Clinical arbovirus screening requires exclusion of a broad range of viruses with as few assays as possible. We present a reverse transcription-PCR (RT-PCR) for the detection of all species of the genus Alphavirus qualified for exclusion screening (limit of detection [LOD], 5 to 100 RNA copies per reaction across all Alphavirus species; detection of viremia down to ca. 10,000 copies per ml).
Grywna
Klaus
K
Institute of Virology, University of Bonn, Bonn, Germany.
Kupfer
Bernd
B
Panning
Marcus
M
Drexler
Jan Felix
JF
Emmerich
Petra
P
Drosten
Christian
C
Kümmerer
Beate M
BM
D000524:Alphavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D018354:Alphavirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*