Isolation and potential immunological characterization of TPSGLVY, a novel bursal septpeptide isolated from the bursa of Fabricius.
The bursa of Fabricius is central immune organ unique to birds, and the extract is immunocompetent in stimulating B cell differentiation and enhancing antibody production. However, except for bursin, the active peptides from the bursa of Fabricius are little reported. In the paper, a novel bursal septpeptide (BSP-II) with the amino acids sequence of TPSGLVY was identified and similar to the MGC53864 protein of Gallus gallus. We investigated the effects of BSP-II on the immune response in terms of the antibodies titers (IgG1 and IgG2alpha), the levels of interferon-gamma and interleukin-4 cytokines, spleen cell lymphocyte proliferation, and the T-lymphocyte subtype composition. It was noteworthy that BSP-II potentiates the Th1 and Th2-type immune responses in dose-dependent manner. BSP-II had specific enhancing effects on the hybridoma SP2/0 cell proliferation at two different serum concentrations (20% and 5%), but had no connection with the dose of BSP-II. The antibody secreting level of hybridoma SP2/0 cells rose in 5% and 20% serum when the concentrations of BSP-II increased. Also, BSP-II had effect on the viabilities of tumor cells (Hela and SP2/0). All the results indicated that BSP-II was able to significantly induce various immune responses and involved in the cell viability of different tumor cell lines. Our observations implied that BSP-II might be a novel biological active factor from the bursa of Fabricius with immunomodulatory activities.
Feng
Xiuli
X
Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China.
Su
Xiaodong
X
Wang
Fangquan
F
Wei
Jianchao
J
Wang
Fengjuan
F
Cao
Ruibing
R
Zhou
Bin
B
Mao
Xiang
X
Zheng
Qisheng
Q
Chen
Puyan
P
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000097:blood / Q000378:metabolism; D000970:Antineoplastic Agents / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D030161:Avian Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification* / Q000494:pharmacology; D002060:Bursa of Fabricius / Q000737:chemistry* / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D016207:Cytokines / Q000097:blood; D005260:Female; D006367:HeLa Cells; D006801:Humans; D006825:Hybridomas; D007111:Immunity, Cellular / Q000187:drug effects; D007155:Immunologic Factors / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification* / Q000494:pharmacology; D009994:Osmolar Concentration; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D000970:Antineoplastic Agents; D030161:Avian Proteins; D016207:Cytokines; D007155:Immunologic Factors; D009842:Oligopeptides; C553590:threonyl-prolyl-seryl-glycyl-leucyl-valyl-tyrosine