Characterization and localization of dynein and myosins V and VI in the ovaries of queen bees.
The presence of myosin and dynein in the ovaries of both Apis mellifera and Scaptotrigona postica was investigated in extracts and in histological sections. In the ovary extracts, motor proteins, myosins V, VI and dynein were detected by Western blot. In histological sections, they were detected by immunocytochemistry, using a mouse monoclonal antibody against the intermediary chain of dynein and a rabbit polyclonal antibody against the myosin V head domain. The myosin VI tail domain was recognized by a pig polyclonal antibody. The results show that these molecular motors are expressed in the ovaries of both bee species with few differences in location and intensity, in regions where movement of substances is expected during oogenesis. The fact that antibodies against vertebrate proteins recognize proteins of bee species indicates that the specific epitopes are evolutionarily well preserved.
Patricio
Karina
K
Department of Zootechny, Laboratory of Biotechnology, ESALQ-USP 13418-900 Piracicaba, SP, Brazil.
Calábria
Luciana Karen
LK
Peixoto
Pablo Marco
PM
Espindola
Foued Salmen
FS
Da Cruz-Landim
Carminda
C
D000818:Animals; D000911:Antibodies, Monoclonal; D001516:Bees / Q000378:metabolism*; D015415:Biomarkers; D015153:Blotting, Western; D004398:Dyneins / Q000276:immunology / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D046529:Microscopy, Electron, Transmission; D018995:Myosin Heavy Chains / Q000276:immunology / Q000378:metabolism*; D024701:Myosin Type V / Q000276:immunology / Q000378:metabolism*; D009866:Oogenesis; D010053:Ovary / Q000378:metabolism / Q000502:physiology; D011817:Rabbits
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D000939:Epitopes; C089785:myosin VI; D024701:Myosin Type V; D018995:Myosin Heavy Chains; D004398:Dyneins