Colwellia chukchiensis sp. nov., a psychrotolerant bacterium isolated from the Arctic Ocean.
A novel psychrotolerant bacterial strain, BCw111(T), was isolated from seawater samples from the Chukchi Sea in the Arctic Ocean. Cells of strain BCw111(T) were Gram-negative, motile, facultatively anaerobic, curved rods and were able to grow at 0-30 °C (optimum 23-25 °C). Strain BCw111(T) had Q-8 as the major respiratory quinone and contained iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c (28.13 %), C(16 : 0) (13.28 %) and C(17 : 1) (12.90 %) as the major cellular fatty acids. The genomic DNA G+C content was 41.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BCw11(T) formed a distinct lineage within the genus Colwellia and exhibited the highest 16S rRNA gene sequence similarity with Colwellia polaris 537(T) (97.8 %) and Colwellia aestuarii SMK-10(T) (97.1 %). Based on phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness, a novel species, Colwellia chukchiensis sp. nov., is proposed. The type strain is BCw111(T) ( = CGMCC 1.9127(T)  = LMG 25329(T)  = DSM 22576(T)).
Yu
Yong
Y
SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, PR China.
Li
Hui-Rong
HR
SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, PR China.
Zeng
Yin-Xin
YX
SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, PR China.
D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000693:Anaerobiosis; D001110:Arctic Regions; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009792:Oceans and Seas; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S