title: Interactions of antibiotics and extracts of Helichrysum pedunculatum against bacteria implicated in wound infections.
abstract: The effect of combinations of the crude acetone and aqueous extracts of Helichrysum pedunculatum leaves and eight antibiotics was determined by means of checkerboard and time-kill methods. In the checkerboard method, synergy of 45.8% was observed, being independent of Gram reaction, with combinations in the aqueous extract yielding largely (18.8%) antagonistic interactions. The time-kill assay detected synergy (45.8%) that was also independent of Gram reaction with a potentiation of more than 3 orders of the bactericidal activity of the test antibiotics. The crude leaf extracts of H. pedunculatum could thus be considered to be potential source of a broad-spectrum antibiotic-resistance-modifying compounds.
authors.affiliation: Department of Biochemistry and Microbiology, University of Fort Hare, Alice, 5700, South Africa.
authors.forename: O A
authors.initials: OA
authors.lastname: Aiyegoro
authors.forename: A J
authors.initials: AJ
authors.lastname: Afolayan
authors.forename: A I
authors.initials: AI
authors.lastname: Okoh
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D036562:Helichrysum / Q000737:chemistry*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D014946:Wound Infection / Q000188:drug therapy / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts