USA hCG reference service, 10-year report.
BACKGROUND
The USA hCG Reference Service has been dealing with cases of persistent low levels of hCG and gestational trophoblastic diseases for 10years. Here we present the complete experience.


METHODS
Total hCG in serum and urine was measured using the Siemen's Immulite 1000 assay. Hyperglycosylated hCG, nicked hCG, free ss-subunit and ss-core fragment were measured using microtiterplate assays with antibodies B152, B151, FBT11 and B210, respectively.


RESULTS
The USA hCG Reference Service has identified 83 cases of false-positive hCG, 71 cases of aggressive gestational trophoblastic disease (GTD), 52 cases of minimally invasive GTD, 168 cases of quiescent GTD and 22 cases of placenta site trophoblastic tumor (PSTT). In addition, 103 cases of pituitary hCG have been identified, 60 cases of nontrophoblastic tumor, 4 cases of inherited hCG and 2 cases of Munchausen's syndrome. This is 565 cases total. Multiple new methods are described and tested for diagnosing all of these disorders.


CONCLUSIONS
The USA hCG Reference Service experience shows new methods for detecting multiple hCG-related disorders and recommends new approaches for detecting these hCG-related disorders.
Cole
Laurence A
LA
USA hCG Reference Service, Department of Obstetrics and Gynecology, University of New Mexico, Albuquerque, NM 87109, USA. larry@hcglab.com
Laidler
Laura L
LL
Muller
Carolyn Y
CY
D000328:Adult; D006063:Chorionic Gonadotropin / Q000097:blood* / Q000652:urine; D005189:False Positive Reactions; D005260:Female; D031901:Gestational Trophoblastic Disease / Q000097:blood / Q000652:urine; D006801:Humans; D011247:Pregnancy; D018245:Trophoblastic Tumor, Placental Site / Q000097:blood / Q000652:urine
D006063:Chorionic Gonadotropin