title: Retinoic acid and dimethyl sulfoxide promote efficient delivery of transgenes to mouse skin by topically transdermal penetration.
abstract: Simple and efficient gene transfer to the skin would facilitate many local and systemic gene therapy applications. This study reports a novel approach that allows expression of plasmid DNA in epidermis and hair follicle cells with dimethyl sulfoxide (DMSO) after pre-treatment with depilation and retinoic acid (RA) for the purposes of gene therapy. This study investigated the transdermal efficacy of gene to mouse skin when utilizing DMSO after RA pre-treatment. Retinoic acid pre-treatment can increase the efficiency of transfection. This finding indicates that one can more effectively and much less expensively make use of genes therapy to treat diseases of the hair and skin.
authors.affiliation: State Key Laboratory of Biotherapy,West China Hospital, Sichuan University, Chengdu,PR China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Chen
authors.forename: Yinbing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Changyong
authors.initials: C
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xikun
authors.initials: X
authors.lastname: Zhou
authors.forename: Taoyou
authors.initials: T
authors.lastname: Zhou
authors.forename: Yongqiu
authors.initials: Y
authors.lastname: Mao
authors.forename: Bing
authors.initials: B
authors.lastname: Kan
authors.forename: Yu-Quan
authors.initials: YQ
authors.lastname: Wei
authors.forename: Jiong
authors.initials: J
authors.lastname: Li
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage / Q000737:chemistry*; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000191:economics / Q000379:methods*; D018859:Hair Follicle / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009994:Osmolar Concentration; D010539:Permeability / Q000187:drug effects; D014677:Pharmaceutical Vehicles; D010957:Plasmids / Q000097:blood / Q000235:genetics / Q000493:pharmacokinetics; D011292:Premedication; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012871:Skin Diseases / Q000628:therapy; D014162:Transfection / Q000379:methods*; D019076:Transgenes; D014212:Tretinoin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D014677:Pharmaceutical Vehicles; D014212:Tretinoin; D004121:Dimethyl Sulfoxide