Antimicrobial activity and essential oil composition of a new T. argyrophyllum (C. Koch) Tvzel var. argyrophyllum chemotype.
Water-distilled essential oils from flowers and stems of Tanacetum argyrophyllum var. argyrophyllum from Turkey were analyzed by GC and GC/MS. The flower oil of T. argyrophyllum var. argyrophyllum was characterized with camphor 29.7%, borneol 12.0%, 1,8-cineole 8.4% and bornyl acetate 6.1%. Stem oil was characterized with camphor 26.6%, 1,8-cineole 17.5%, and borneol 15.0%. Our previous research and literature on the essential oil of this plant reported oils with high thujone content unlike the present study. Antibacterial activity of the oils were evaluated for five Gram-positive and five Gram-negative bacteria by using a broth microdilution assay. The highest inhibitory activity was observed against Bacillus cereus for stem oil (125 microg/mL) when compared with positive control chloramphenicol it showed the same inhibition potency. However, the same oil showed lower inhibitory activity against B. subtilis. The flower oil did not show significant activity against the tested microorganisms. DPPH radical scavenging activity of the T. argyrophyllum var. argyrophyllum oil was investigated at 15 and 10 mg/mL concentrations. However, the oils did not show any significant activity when compared to positive control alpha-tocopherol. Both of the oils showed toxicity to Vibrio fischeri in the TLC-bioluminescence assay.
Polatoglu
Kaan
K
Faculty of Science & Letters, Department of Biology, 34210, Yildiz Technical University, Istanbul, Turkey. kaanpolatoglu@gmail.com
Demirci
Fatih
F
Demirci
Betül
B
Gören
Nezhun
N
Can Baser
Kemal Hüsnü
KH
D048908:Aliivibrio fischeri / Q000187:drug effects; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001892:Camphanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002164:Camphor / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D003511:Cyclohexanols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000077591:Eucalyptol; D035264:Flowers / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009822:Oils, Volatile / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D031203:Tanacetum / Q000737:chemistry*; D014421:Turkey; D024502:alpha-Tocopherol / Q000494:pharmacology
D000890:Anti-Infective Agents; D001892:Camphanes; D003511:Cyclohexanols; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils; C071528:bornyl acetate; D002164:Camphor; D024502:alpha-Tocopherol; C022871:isoborneol; D000077591:Eucalyptol