Cephalometric analysis of craniofacial malformations in newborn mice with cleft palate induced by retinoic acid.
OBJECTIVE
To determine changes on craniofacial growth morphometrically in newborn mice with cleft palate induced by retinoic acid. DESIGN, SETTING, PARTICIPANTS, INTERVENTIONS: Gestation day 10 or 12 pregnant female C57BL/6N mice were given a single dose of all-trans retinoic acid (atRA) by gastric intubations via oral gavage. Sixty newborn mice with cleft palate (CP), 52 without CP from the experimental group, and 30 without CP from the control group were collected, and lateral cephalograms were taken of all of the mice.


METHODS
Cephalometric analysis of the craniofacial skeleton was performed by means of a personal computer.


RESULTS
Inhibition of craniofacial growth was found in the experimental groups but not in the control groups. In the maxillary bone and mandible, the amount of growth was significantly reduced.


CONCLUSIONS
These results suggest that craniofacial growth is inhibited in newborn mice with cleft palate induced by retinoic acid.
Chen
Mu
M
Huang
Hong-Zhang
HZ
Zeng
Dong-Lin
DL
Wang
Da-Wei
DW
D000818:Animals; D000831:Animals, Newborn; D002508:Cephalometry / Q000379:methods*; D002972:Cleft Palate / Q000139:chemically induced / Q000000981:diagnostic imaging*; D019465:Craniofacial Abnormalities / Q000139:chemically induced / Q000000981:diagnostic imaging*; D004622:Embryo, Mammalian / Q000187:drug effects; D005260:Female; D005333:Fetus / Q000187:drug effects; D005865:Gestational Age; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D011859:Radiography; D014212:Tretinoin / Q000633:toxicity*
D014212:Tretinoin