title: Validation study of a rapid ELISA for detection of deoxynivalenol in wheat, barley, malted barley, corn, oats, and rice. Performance Tested Method 090901.
abstract: Neogen Corp. developed the Veratox DON test kit for the detection ofdeoxynivalenol (DON). The purpose of this study was to validate the method under the requirements of the AOAC Research Institute Performance Tested Methods (PTM) program. There are two AOAC Official Methods for DON detection: 986.17 and 986.18, the first of which is a TLC method and the second a GC method. A rapid method (PTM 000701) has also been performance tested by the AOAC Research Institute. One of the most widely used reference methods; however, is a GC method with electron capture detection that is referred to as the reference method in this paper. Although considered the reference method, the GC procedure is complicated and requires the investment of both expensive equipment and a highly skilled technician. A rapid (e.g., ELISA) test kit needs to be validated by the AOAC Research Institute. The Veratox 2/3 method is highly reproducible with average CV values < 10%, and is very accurate, showing > 97% correlation to reference methods.
authors.affiliation: Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA. tlupo@neogen.com
authors.forename: Anthony
authors.initials: A
authors.lastname: Lupo
authors.forename: Chris
authors.initials: C
authors.lastname: Roebuck
authors.forename: Ken
authors.initials: K
authors.lastname: Settimo
authors.forename: Anna
authors.initials: A
authors.lastname: Quain
authors.forename: Justina
authors.initials: J
authors.lastname: Kennedy
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abouzied
mesh_terms: D018554:Avena / Q000378:metabolism*; D002623:Chemistry Techniques, Analytical; D002849:Chromatography, Gas / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D001467:Hordeum / Q000378:metabolism*; D007753:Laboratories / Q000592:standards; D012275:Oryza / Q000378:metabolism*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013696:Temperature; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000378:metabolism*; D014481:United States; D014486:United States Food and Drug Administration; D003313:Zea mays / Q000378:metabolism*
chemical_list: D011933:Reagent Kits, Diagnostic; D014255:Trichothecenes; C007262:deoxynivalenol