Novel immunogenic antigens increase classification accuracy in meningioma to 93.84%.
There is growing evidence that simultaneous analysis of multiple autoantibody reactions can be utilized for diagnosis of neoplasms. Using a set of 57 meningioma-associated antigens, we recently separated meningioma patients from individuals without known disease with an accuracy of 90.3%. Here, we ask whether a largely increased set of immunogenic antigens can further improve this discrimination. We used an array with 1,827 human recombinant clones and measured reactivity of serum autoantibodies against the clones by a novel automated image analysis procedure. We were able to separate meningioma sera from sera of healthy controls with a specificity of 95.62%, a sensitivity of 91.83% and an accuracy of 93.84%. Of the analyzed clones, 23 in-frame clones were highly informative for the classification of meningioma vs. normal sera as shown by their AUC values. These results demonstrate that the accuracy of a serum-based diagnostic can be readily and considerably improved by screening extended sets of proteins.
Ludwig
Nicole
N
Department of Human Genetics, Medical School, Saarland University, Homburg/Saar, Germany. n.ludwig@mx.uni-saarland.de
Keller
Andreas
A
Heisel
Sabrina
S
Leidinger
Petra
P
Rheinheimer
Stefanie
S
Andres
Claudia
C
Stephan
Bernhard
B
Steudel
Wolf-Ingo
WI
Donauer
Erich
E
Graf
Norbert
N
Burgeth
Bernhard
B
Weickert
Joachim
J
Lenhof
Hans-Peter
HP
Meese
Eckart
E
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000145:classification* / Q000276:immunology / Q000378:metabolism*; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D015723:Gene Library; D005910:Glioma / Q000097:blood / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000097:blood / Q000235:genetics / Q000276:immunology*; D008579:Meningioma / Q000097:blood / Q000235:genetics / Q000276:immunology*; D008875:Middle Aged; D011379:Prognosis; D012680:Sensitivity and Specificity; D055815:Young Adult
D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor