Pancreatic injury in hepatic alcohol dehydrogenase-deficient deer mice after subchronic exposure to ethanol.
Pancreatitis caused by activation of digestive zymogens in the exocrine pancreas is a serious chronic health problem in alcoholic patients. However, mechanism of alcoholic pancreatitis remains obscure due to lack of a suitable animal model. Earlier, we reported pancreatic injury and substantial increases in endogenous formation of fatty acid ethyl esters (FAEEs) in the pancreas of hepatic alcohol dehydrogenase (ADH)-deficient (ADH(-)) deer mice fed 4% ethanol. To understand the mechanism of alcoholic pancreatitis, we evaluated dose-dependent metabolism of ethanol and related pancreatic injury in ADH(-) and hepatic ADH-normal (ADH(+)) deer mice fed 1%, 2% or 3.5% ethanol via Lieber-DeCarli liquid diet daily for 2months. Blood alcohol concentration (BAC) was remarkably increased and the concentration was ∼1.5-fold greater in ADH(-) vs. ADH(+) deer mice fed 3.5% ethanol. At the end of the experiment, remarkable increases in pancreatic FAEEs and significant pancreatic injury indicated by the presence of prominent perinuclear space, pyknotic nuclei, apoptotic bodies and dilation of glandular ER were found only in ADH(-) deer mice fed 3.5% ethanol. This pancreatic injury was further supported by increased plasma lipase and pancreatic cathepsin B (a lysosomal hydrolase capable of activating trypsinogen), trypsinogen activation peptide (by-product of trypsinogen activation process) and glucose-regulated protein 78 (endoplasmic reticulum stress marker). These findings suggest that ADH-deficiency and high alcohol levels in the body are the key factors in ethanol-induced pancreatic injury. Therefore, determining how this early stage of pancreatic injury advances to inflammation stage could be important for understanding the mechanism(s) of alcoholic pancreatitis.
Kaphalia
Bhupendra S
BS
Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Bhopale
Kamlesh K
KK
Kondraganti
Shakuntala
S
Wu
Hai
H
Boor
Paul J
PJ
Ansari
G A Shakeel
GA
D000079:Acetaldehyde / Q000097:blood / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000172:deficiency* / Q000378:metabolism; D000818:Animals; D002401:Cathepsin B / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D004721:Endoplasmic Reticulum / Q000378:metabolism; D000431:Ethanol / Q000097:blood / Q000633:toxicity*; D005019:Ethyl Ethers / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000097:blood / Q000378:metabolism; D008049:Lipase / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000097:blood / Q000378:metabolism; D051379:Mice; D009842:Oligopeptides / Q000097:blood / Q000378:metabolism; D010179:Pancreas / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D019512:Pancreatitis, Alcoholic / Q000097:blood / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology
D005019:Ethyl Ethers; D005227:Fatty Acids; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D009842:Oligopeptides; C018551:glucose-regulated proteins; C080396:trypsinogen activation peptide; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D008049:Lipase; D002401:Cathepsin B; D000079:Acetaldehyde