A novel nucleotide insertion in S gene of hepatitis B virus in a chronic carrier.
Hepatitis B virus DNA was extracted from serum of a chronic carrier and polymerase chain reaction was performed on S gene. Direct sequencing showed a variant HBsAg with additional 4-amino acid insertion, and clone sequencing confirmed the mixture of variant HBsAg and wildtype HBsAg. Of 16 clones with 12-nucleotide insertion, 15 clones had identical AGAACAACACAA insertion between nucleotide 494 and nucleotide 495, and one clone had GGAACAACTCAA insertion in the same position plus 3-nucleotide deletion from nucleotide 491 to nucleotide 493. S114T, C121Y, T126S/A, Q129K, G130R, T131N, M133T, G145R, N146D substitution and premature stop codon were also found in those clones. However, the origin of HBV with 4-amino acid insertion in HBsAg was unclear.
Tong
Wenbin
W
Institute of Microbiological Detection, Sichuan Center for Disease Control and Prevention, Chengdu, PR China. tong_wen_bin@163.com
Sun
Li
L
He
Jilan
J
He
Shusen
S
Du
Fei
F
D000368:Aged; D000595:Amino Acid Sequence; D001483:Base Sequence; D002353:Carrier State / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000737:chemistry / Q000235:genetics*; D006515:Hepatitis B virus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010802:Phylogeny; D016415:Sequence Alignment; D017384:Sequence Deletion
D006514:Hepatitis B Surface Antigens