title: Short communication: Antiproliferative effect of wild Lactobacillus strains isolated from fermented foods on HT-29 cells.
abstract: In vitro studies, animal models, epidemiology, and human intervention studies provide evidence that some lactic acid bacteria can reduce the risk of certain cancers. In this study, heat-killed bacterial cells, genomic DNA, and cell wall of 7 wild Lactobacillus strains isolated from traditional fermented foods in western China were tested in vitro for cytotoxicity on colonic cancer cell line HT-29 by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The heat-killed bacterial cells, genomic DNA, and cell wall of the 7 strains exhibited direct antiproliferative activities against HT-29 cells. Among the strains, the cellular components of Lactobacillus coryniformis ssp. torquens T3L exerted marked antiproliferative activities against HT-29 cells. The maximum inhibition rates of HT-29 cells by the heat-killed bacterial cells (1x10(7) cfu/mL), cell wall (20 microg of protein/mL) and genomic DNA (100 microg/mL) of L. coryniformis ssp. torquens T3L were 30, 44.9, and 35.9%, respectively. The results indicate that the heat-killed bacterial cells, cell wall, and genomic DNA of the 7 wild Lactobacillus strains could inhibit the growth of HT-29 cells.
authors.affiliation: Department of Food Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Tuo
authors.forename: L W
authors.initials: LW
authors.lastname: Zhang
authors.forename: H X
authors.initials: HX
authors.lastname: Yi
authors.forename: Y C
authors.initials: YC
authors.lastname: Zhang
authors.forename: W Q
authors.initials: WQ
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Han
authors.forename: M
authors.initials: M
authors.lastname: Du
authors.forename: Y H
authors.initials: YH
authors.lastname: Jiao
authors.forename: S M
authors.initials: SM
authors.lastname: Wang
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002473:Cell Wall / Q000502:physiology; D043302:Cultured Milk Products / Q000502:physiology*; D004269:DNA, Bacterial / Q000502:physiology; D019073:HT29 Cells / Q000187:drug effects*; D006358:Hot Temperature; D006801:Humans; D007778:Lactobacillus / Q000502:physiology*; D008826:Microbial Sensitivity Tests
chemical_list: D004269:DNA, Bacterial