Human iron-sulfur cluster assembly, cellular iron homeostasis, and disease.
Iron-sulfur (Fe-S) proteins contain prosthetic groups consisting of two or more iron atoms bridged by sulfur ligands, which facilitate multiple functions, including redox activity, enzymatic function, and maintenance of structural integrity. More than 20 proteins are involved in the biosynthesis of iron-sulfur clusters in eukaryotes. Defective Fe-S cluster synthesis not only affects activities of many iron-sulfur enzymes, such as aconitase and succinate dehydrogenase, but also alters the regulation of cellular iron homeostasis, causing both mitochondrial iron overload and cytosolic iron deficiency. In this work, we review human Fe-S cluster biogenesis and human diseases that are caused by defective Fe-S cluster biogenesis. Fe-S cluster biogenesis takes place essentially in every tissue of humans, and products of human disease genes, including frataxin, GLRX5, ISCU, and ABCB7, have important roles in the process. However, the human diseases, Friedreich ataxia, glutaredoxin 5-deficient sideroblastic anemia, ISCU myopathy, and ABCB7 sideroblastic anemia/ataxia syndrome, affect specific tissues, while sparing others. Here we discuss the phenotypes caused by mutations in these different disease genes, and we compare the underlying pathophysiology and discuss the possible explanations for tissue-specific pathology in these diseases caused by defective Fe-S cluster biogenesis.
Ye
Hong
H
Molecular Medicine Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Rouault
Tracey A
TA
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000756:Anemia, Sideroblastic / Q000235:genetics; D000818:Animals; D002524:Cerebellar Ataxia / Q000235:genetics; D005621:Friedreich Ataxia / Q000235:genetics; D054477:Glutaredoxins / Q000235:genetics; D006706:Homeostasis; D006801:Humans; D007501:Iron / Q000378:metabolism*; D033862:Iron-Binding Proteins / Q000235:genetics; D007506:Iron-Sulfur Proteins / Q000235:genetics / Q000378:metabolism*; D017240:Mitochondrial Myopathies / Q000235:genetics; D009154:Mutation; D013577:Syndrome; D000096923:Frataxin
D018528:ATP-Binding Cassette Transporters; D054477:Glutaredoxins; D007501:Iron; D033862:Iron-Binding Proteins; D007506:Iron-Sulfur Proteins; D000096923:Frataxin; C113037:ABCB7 protein, human; C516005:GLRX protein, human; C506160:ISCU protein, human