title: An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase.
abstract: Recently, a codon improved version of the Flpe site specific recombinase, termed Flpo, was reported as having greatly improved performance in mammalian cell applications. However, the degree of improvement could not be estimated because essentially no Flpe activity was observed. Here, we compare Flpe and Flpo accurately in a mammalian cell assay to estimate that Flpo is about five times more active than Flpe and similar to Cre and Dre. Consequently, we generated a Flpo deleter mouse line from the JM8 C57Bl/6 ES cells used in the EUCOMM and KOMP systematic knock-out programs. In breeding experiments, we show that the Flpo deleter delivers complete recombination using alleles that are incompletely recombined by a commonly used Flpe deleter. This indicates that the Flpo deleter is more efficient.
authors.affiliation: Genomics, BioInnovationsZentrum, Technische Universität Dresden, Am Tatzberg 47, Dresden, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Kranz
authors.forename: Jun
authors.initials: J
authors.lastname: Fu
authors.forename: Kristin
authors.initials: K
authors.lastname: Duerschke
authors.forename: Stefanie
authors.initials: S
authors.lastname: Weidlich
authors.forename: Ronald
authors.initials: R
authors.lastname: Naumann
authors.forename: A Francis
authors.initials: AF
authors.lastname: Stewart
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Anastassiadis
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D002478:Cells, Cultured; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D055786:Gene Knockout Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D014162:Transfection
chemical_list: D007527:Isoenzymes; D049452:Green Fluorescent Proteins; D004254:DNA Nucleotidyltransferases; C077570:Site-specific recombinase