[Role of NADPH oxidase in endothelial cell dysfunction induced by endothelial microparticles].
OBJECTIVE
To explore the correlation between circulating endothelial microparticles (EMPs) and flow-mediated dialation (FMD) in patients with coronary artery disease (CAD), and investigate the role of NADPH oxidase in endothelial cell dysfunction caused by EMPs.


METHODS
Fifteen patients with CAD and 15 at high risks of CAD were tested for the level of EMPs and FMD and other biochemical indices, and the correlation between the indices were analyzed. EMPs obtained from cultured human umbilical vein endothelial cells (HUVECs) were phenotyped and used to stimulate the HUVECs, whose ROS and NO production was tested.


RESULTS
Endothelial dilation function could be damaged by circulating EMPs in CAD patients. Dysfunction of HUVECs caused by 10(5)/ml EMPs could be reversed by pretreatment with 20 micromol/L apocynin, a NADPH oxidase inhibitor.


CONCLUSIONS
Endothelial dialation function of the endothelial cells can be damaged by circulating EMPs in patients with CAD in association with NADPH oxidase activation.
Cheng
Fei
F
Department of Hypertension and Vascular Disease, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. mdcf2006@126.com
Tao
Jun
J
Feng
Jian-qiang
JQ
Yang
Chun-tao
CT
Wang
Yan
Y
Zhang
Yuan-yuan
YY
Zhang
Xiao-lin
XL
D000328:Adult; D000368:Aged; D055252:Cell-Derived Microparticles / Q000187:drug effects* / Q000378:metabolism*; D002478:Cells, Cultured; D003327:Coronary Disease / Q000473:pathology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000378:metabolism*; D014471:Umbilical Veins / Q000166:cytology
D019255:NADPH Oxidases