Opposite effects of arachidonic acid and of its hydroperoxides on brain soluble guanylate cyclase activity.
Purified soluble guanylate cyclase from rat brain was found to be directly activated by arachidonic acid. Arachidonic acid hydroperoxides, obtained by the action of lipoxygenase, were inhibitory. An inhibition of soluble guanylate cyclase and a decrease of cyclic GMP content were also observed in pure neuronal cultures treated with lipoxygenase. The results suggest that lipoxygenase products are not involved in the production of cyclic GMP in neurons. This is in contrast with other tissues for which it is established that hormone-induced guanylate cyclase activation is regulated by lipoxygenase-catalysed metabolism of polyunsaturated fatty acids.
Louis
J C
JC
Centre de Neurochimie du CNRS, 5 rue Blaise Pascal, 67084 Strasbourg, France.
Basset
P
P
Revel
M O
MO
Vincendon
G
G
Zwiller
J
J