title: Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.
abstract: The plus-end microtubule binding proteins (+TIPs) play an important role in the regulation of microtubule stability and cell polarity during interphase. In S. pombe, the CLIP-170 like protein Tip1, together with the kinesin Tea2, moves along the microtubules towards their plus ends. Tip1 also requires the EB1 homolog Mal3 to localize to the microtubule tips. Given the requirement for Tip1 for microtubule stability, we have investigated its role during spindle morphogenesis and chromosome movement. Loss of Tip1 affects metaphase plate formation and leads to the activation of the spindle assembly checkpoint. In the absence of Tip1 we also observed the appearance of lagging chromosomes, which do not influence the normal rate of spindle elongation. Our results suggest that S. pombe Tip1/CLIP170 is directly or indirectly required for correct chromosome poleward movement independently of Mal3/EB1.
authors.affiliation: LBCMCP, CNRS, Université de Toulouse, Toulouse, France.
authors.forename: Sherilyn
authors.initials: S
authors.lastname: Goldstone
authors.forename: Céline
authors.initials: C
authors.lastname: Reyes
authors.forename: Guillaume
authors.initials: G
authors.lastname: Gay
authors.forename: Thibault
authors.initials: T
authors.lastname: Courthéoux
authors.forename: Marion
authors.initials: M
authors.lastname: Dubarry
authors.forename: Sylvie
authors.initials: S
authors.lastname: Tournier
authors.forename: Yannick
authors.initials: Y
authors.lastname: Gachet
mesh_terms: D016764:Cell Polarity / Q000187:drug effects; D015825:Chromosomes, Fungal / Q000378:metabolism*; D017353:Gene Deletion; D006360:Heat-Shock Proteins / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D018386:Kinetochores / Q000187:drug effects / Q000378:metabolism; D008677:Metaphase / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D010641:Phenotype; D021381:Protein Transport / Q000187:drug effects; D012568:Schizosaccharomyces / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D008941:Spindle Apparatus / Q000187:drug effects / Q000378:metabolism; D013827:Thiabendazole / Q000494:pharmacology
chemical_list: D006360:Heat-Shock Proteins; D007381:Intermediate Filament Proteins; D029702:Schizosaccharomyces pombe Proteins; C114142:tip1 protein, S pombe; D013827:Thiabendazole