Platelet-rich plasma in bone repair of irradiated tibiae of Wistar rats.
OBJECTIVE
To evaluate the influence of PRP addition on bone repair of circular defects created in irradiated tibiae of rats by histometric analysis.


METHODS
Sixty male Wistar rats had the right tibiae irradiated with 30 Gy. After 30 days monocortical defects were created and platelet-rich plasma was applied in 30 rats. In the control group defects were created but not filled. The animals were desanguinated after 4, 7, 14, 21, 56 and 84 days and the tibiae removed for histological processing.


RESULTS
There was a tendency in the PRP group to increased bone neoformation from 14-days to 84-days; in the control group increased bone neoformation was not seen after 21 days or later.


CONCLUSIONS
The addition of platelet-rich plasma had a beneficial effect in the initial cellular regeneration period and enhanced bone formation in later periods when compared to control.
Gumieiro
Emne Hammoud
EH
Department of Otorhinolaringology and Head and Neck Surgery, UNIFESP, São Paulo, Brazil. gumieiro@uol.com.br <gumieiro@uol.com.br>
Abrahão
Márcio
M
Jahn
Ricardo Schmitutz
RS
Segretto
Helena
H
Alves
Maria Tereza de Seixas
MT
Nannmark
Ulf
U
Granström
Gösta
G
Dib
Luciano Lauria
LL
D000818:Animals; D001861:Bone Regeneration / Q000502:physiology* / Q000528:radiation effects; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology*; D008297:Male; D010976:Platelet Count; D053657:Platelet-Rich Plasma; D011833:Radiation Injuries, Experimental; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000528:radiation effects*; D013997:Time Factors
D036341:Intercellular Signaling Peptides and Proteins