title: Non-Mendelian determinant [ISP+] in yeast is a nuclear-residing prion form of the global transcriptional regulator Sfp1.
abstract: Four protein-based genetic determinants or prions-[SWI(+)], [MCA], [OCT(+)], and [MOT3(+)]-are recent additions to the list of well-known Saccharomyces cerevisiae prions, [PSI(+)], [URE3], and [PIN(+)]. A rapid expansion of this list may indicate that many yeast proteins can convert into heritable prion forms and underscores a problem of prion input into cellular physiology. Here, we prove that the global transcriptional regulator Sfp1 can become a prion corresponding to the prion-like determinant [ISP(+)] described earlier. We show that SFP1 deletion causes an irreversible [ISP(+)] loss, whereas increased SFP1 expression induces [ISP(+)] appearance. Cells that display the [ISP(+)] phenotype contain the aggregated form of Sfp1. Indeed, these aggregates demonstrate a nuclear location. We also show that the phenotypic manifestation of Sfp1 prionization differs from the manifestation of SFP1 deletion. These properties and others distinguish [ISP(+)] from yeast prions described to date.
authors.affiliation: Department of Genetics, St. Petersburg State University, St. Petersburg, Russia.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Rogoza
authors.forename: Alexander
authors.initials: A
authors.lastname: Goginashvili
authors.forename: Sofia
authors.initials: S
authors.lastname: Rodionova
authors.forename: Maxim
authors.initials: M
authors.lastname: Ivanov
authors.forename: Olga
authors.initials: O
authors.lastname: Viktorovskaya
authors.forename: Alexander
authors.initials: A
authors.lastname: Rubel
authors.forename: Kirill
authors.initials: K
authors.lastname: Volkov
authors.forename: Ludmila
authors.initials: L
authors.lastname: Mironova
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D010641:Phenotype; D011328:Prions / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D011328:Prions; C117421:SFP1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors