title: Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model.
abstract: Matrigel promotes angiogenesis in the myocardium from ischemic injury and prevents remodelling of the left ventricle. We assessed the therapeutic efficacy of intracardiac matrigel injection and matrigel-mediated stem cell homing in a rat myocardial infarction (MI) model. Following MI, matrigel (250 μl) or phosphate-buffered solution (PBS) was delivered by intracardiac injection. Compared to the MI control group (MI-PBS), matrigel significantly improved left ventricular function (n= 11, P < 0.05) assessed by pressure-volume loops after 4 weeks. There is no significant difference in infarct size between MI-matrigel (MI-M; 21.48 ± 1.49%, n = 10) and MI-PBS hearts (20.98 ± 1.25%, n = 10). The infarct wall thickness of left ventricle is significantly higher (P < 0.01) in MI-M (0.72 ± 0.02 mm, n = 10) compared with MI-PBS (0.62 ± 0.02 mm, n = 10). MI-M hearts exhibited higher capillary density (border 130.8 ± 4.7 versus 115.4 ± 6.0, P < 0.05; vessels per high-power field [HPF; 400×], n = 6) than MI-PBS hearts. c-Kit(+) stem cells (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit(+) cells per HPF [630×], n = 5, P < 0.05) and CD34(+) cells (13.0 ± 1.51 versus 5.6 ± 0.68 CD34(+) cells per HPF [630×], n = 5, P < 0.01) were significantly more numerous in MI-M than in MI-PBS in the infarcted hearts (n = 5, P < 0.05). Intracardiac matrigel injection restores myocardial functions following MI, which may attribute to the improved recruitment of CD34(+) and c-Kit(+) stem cells.
authors.affiliation: Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.
authors.forename: Lailiang
authors.initials: L
authors.lastname: Ou
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Heiko
authors.initials: H
authors.lastname: Sorg
authors.forename: Dario
authors.initials: D
authors.lastname: Furlani
authors.forename: Ralf
authors.initials: R
authors.lastname: Gäbel
authors.forename: Peter
authors.initials: P
authors.lastname: Mark
authors.forename: Christian
authors.initials: C
authors.lastname: Klopsch
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Karola
authors.initials: K
authors.lastname: Lützow
authors.forename: Andreas
authors.initials: A
authors.lastname: Lendlein
authors.forename: Klaus
authors.initials: K
authors.lastname: Wagner
authors.forename: Doris
authors.initials: D
authors.lastname: Klee
authors.forename: Andreas
authors.initials: A
authors.lastname: Liebold
authors.forename: Ren-Ke
authors.initials: RK
authors.lastname: Li
authors.forename: Deling
authors.initials: D
authors.lastname: Kong
authors.forename: Gustav
authors.initials: G
authors.lastname: Steinhoff
authors.forename: Nan
authors.initials: N
authors.lastname: Ma
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000503:physiopathology; D002465:Cell Movement / Q000187:drug effects*; D003094:Collagen / Q000008:administration & dosage / Q000627:therapeutic use; D004195:Disease Models, Animal; D004338:Drug Combinations; D006439:Hemodynamics / Q000187:drug effects; D007273:Injections, Intramuscular; D007797:Laminin / Q000008:administration & dosage / Q000627:therapeutic use; D008026:Ligation; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000503:physiopathology; D009206:Myocardium / Q000473:pathology*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011509:Proteoglycans / Q000008:administration & dosage / Q000627:therapeutic use; D051381:Rats; D011919:Rats, Inbred Strains; D013234:Stem Cells / Q000502:physiology; D016277:Ventricular Function, Left / Q000187:drug effects
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen