title: Continuous perfusion of donor hearts with oxygenated blood cardioplegia improves graft function.
abstract: Donor hearts cannot be preserved beyond 6h using cold storage (CS). Improving preservation methods may permit prolonged storage of donor heart. We compared graft function in large animal model after prolonged preservation (8h) using continuous perfusion (CP) and CS method. Twenty-four miniature pigs were used as donors and recipients. Donor hearts were either stored in University of Wisconsin solution (UW solution) for 8h at 0-4°C (CS group, n=6) or were continuously perfused with oxygenated blood cardioplegia at 26°C for 8h (CP group, n=6). After preservation, hearts were transplanted into recipients and reperfused for 3h. Left ventricular (LV) function, cardiac output (CO), malondialdehyde (MDA) and adenosine triphosphate (ATP) levels, and water content were measured. Although water content of CP hearts was higher than that of CS, LV contractility and diastolic function of CP hearts were superior to those of CS. In addition, CP hearts performed better than CS hearts on CO in working heart state. ATP was better preserved and MDA levels were lower in CP hearts compared with those of CS (P<0.0001). Donor hearts can be preserved longer using continuous perfusion with oxygenated blood cardioplegia and this method prevents time-dependent ischemic injury.
authors.affiliation: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Ansheng
authors.initials: A
authors.lastname: Mo
authors.forename: Zhaoke
authors.initials: Z
authors.lastname: Wen
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Shengjing
authors.initials: S
authors.lastname: Liang
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
mesh_terms: D000241:Adenosine / Q000627:therapeutic use; D000255:Adenosine Triphosphate / Q000737:chemistry; D000493:Allopurinol / Q000627:therapeutic use; D000818:Animals; D002302:Cardiac Output; D005978:Glutathione / Q000627:therapeutic use; D006324:Heart Arrest, Induced / Q000379:methods*; D006333:Heart Failure / Q000628:therapy*; D016027:Heart Transplantation / Q000379:methods*; D007328:Insulin / Q000627:therapeutic use; D007511:Ischemia; D008297:Male; D008315:Malondialdehyde / Q000494:pharmacology; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions / Q000627:therapeutic use; D010477:Perfusion; D011887:Raffinose / Q000627:therapeutic use; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008315:Malondialdehyde; D000493:Allopurinol; D000255:Adenosine Triphosphate; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose