Evaluation of an automated capillary electrophoresis system for serum protein electrophoresis with the determination of gender-specific reference values.
In this report we summarize evaluation data of an automated capillary zone electrophoresis (Capillarys II, Sebia, France) in comparison to a conventional cellulose acetate electrophoresis system (Hite320, Olympus, Germany). Serum samples from 428 blood donors were analysed to evaluate gender-specific reference intervals, which revealed significant percentage differences between male and female. In contrast to conventional electrophoresis methods all lipoproteins co-migrate with the albumin fraction using capillary electrophoresis. Comparisons between both separation methods and quantitative determinations of albumin/total protein show a better correlation between the albumin fraction determined by capillary electrophoresis and the quantitative determinations. A transversal evaluation of serum protein fractions should be performed within one day due to the combination of effects based on serum sample instability and method imprecision.
Lichtinghagen
Ralf
R
Institute of Clinical Chemistry, Hannover Medical School, 30623 Hannover, Germany. Lichtinghagen.Ralf@mh-hannover.de
Pietsch
Daniel
D
Brand
Korbinian
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D001331:Automation; D001782:Blood Donors; D001798:Blood Proteins / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012709:Serum Albumin / Q000032:analysis; D012727:Sex Characteristics; D055815:Young Adult; D005719:gamma-Globulins / Q000032:analysis
D001798:Blood Proteins; D012709:Serum Albumin; D005719:gamma-Globulins