title: A full-length cDNA resource for the pea aphid, Acyrthosiphon pisum.
abstract: Large collections of full-length cDNAs are important resources for genome annotation and functional genomics. We report the creation of a collection of 50 599 full-length cDNA clones from the pea aphid, Acyrthosiphon pisum. Sequencing from 5' and 3' ends of the clones generated 97 828 high-quality expressed sequence tags, representing approximately 9000 genes. These sequences were imported to AphidBase and are shown to play crucial roles in both automatic gene prediction and manual annotation. Our detailed analyses demonstrated that the full-length cDNAs can further improve gene models and can even identify novel genes that are not included in the current version of the official gene set. This full-length cDNA collection can be utilized for a wide variety of functional studies, serving as a community resource for the study of the functional genomics of the pea aphid.
authors.affiliation: HHMI and Department of Ecology and Evolutionary Biology, Princeton University, NJ, USA. shige@nibb.ac.jp <shige@nibb.ac.jp>
authors.forename: S
authors.initials: S
authors.lastname: Shigenobu
authors.forename: S
authors.initials: S
authors.lastname: Richards
authors.forename: A G
authors.initials: AG
authors.lastname: Cree
authors.forename: M
authors.initials: M
authors.lastname: Morioka
authors.forename: T
authors.initials: T
authors.lastname: Fukatsu
authors.forename: T
authors.initials: T
authors.lastname: Kudo
authors.forename: S
authors.initials: S
authors.lastname: Miyagishima
authors.forename: R A
authors.initials: RA
authors.lastname: Gibbs
authors.forename: D L
authors.initials: DL
authors.lastname: Stern
authors.forename: A
authors.initials: A
authors.lastname: Nakabachi
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D001042:Aphids / Q000235:genetics* / Q000472:pathogenicity; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library; D049750:Genome, Insect; D054884:Host-Pathogen Interactions / Q000235:genetics; D008957:Models, Genetic; D018532:Pisum sativum / Q000469:parasitology
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D017931:DNA Primers; D018076:DNA, Complementary