BUD22 affects Ty1 retrotransposition and ribosome biogenesis in Saccharomyces cerevisiae.
A variety of cellular factors affect the movement of the retrovirus-like transposon Ty1. To identify genes involved in Ty1 virus-like particle (VLP) function, the level of the major capsid protein (Gag-p45) and its proteolytic precursor (Gag-p49p) was monitored in a subset of Ty1 cofactor mutants. Twenty-nine of 87 mutants contained alterations in the level of Gag; however, only bud22Delta showed a striking defect in Gag processing. BUD22 affected the +1 translational frameshifting event required to express the Pol proteins protease, integrase, and reverse transcriptase. Therefore, it is possible that the bud22Delta mutant may not produce enough functional Ty1 protease to completely process Gag-p49 to p45. Furthermore, BUD22 is required for 18S rRNA processing and 40S subunit biogenesis and influences polysome density. Together our results suggest that BUD22 is involved in a step in ribosome biogenesis that not only affects general translation, but also may alter the frameshifting efficiency of ribosomes, an event central to Ty1 retrotransposition.
Dakshinamurthy
Arun
A
Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702, USA.
Nyswaner
Katherine M
KM
Farabaugh
Philip J
PJ
Garfinkel
David J
DJ
D015152:Blotting, Northern; D015153:Blotting, Western; D018965:Frameshifting, Ribosomal; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D016254:Mutagenesis, Insertional; D009154:Mutation; D011132:Polyribosomes / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism; D018626:Retroelements / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
C555717:BUD22 protein, S cerevisiae; D015683:Gene Products, gag; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D018626:Retroelements; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C065039:RNA, ribosomal, 25S