Titanium immobilized with an antimicrobial peptide derived from histatin accelerates the differentiation of osteoblastic cell line, MC3T3-E1.
The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. The colonization of P. gingivalis on JH8194-Ti was significantly lower than that on control- and blocking-Ti. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control- and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration.
Makihira
Seicho
S
Division of Oral Health Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
Shuto
Takahiro
T
Division of Oral Health Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
Nikawa
Hiroki
H
Division of Oral Health Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
Okamoto
Keishi
K
Toyo Advanced Technologies Co., LTD. 5-3-38, Ujina-Higashi, Minami-ku, Hiroshima 734-8501, Japan.
Mine
Yuichi
Y
Division of Oral Health Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
Takamoto
Yuko
Y
Division of Oral Health Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
Ohara
Masaru
M
Hiroshima University Hospital, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
Tsuji
Koichiro
K
Two cell Co. LTD., 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D054808:Histatins / Q000737:chemistry* / Q000378:metabolism; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D014025:Titanium / Q000737:chemistry*
D023181:Antimicrobial Cationic Peptides; D050659:Core Binding Factor Alpha 1 Subunit; D054808:Histatins; D055767:Immobilized Proteins; D012333:RNA, Messenger; C493716:Runx2 protein, mouse; D053495:Osteopontin; D014025:Titanium; D000469:Alkaline Phosphatase