Increasing effect of a high dose of PG-1 peptide on the infectivity of Chlamydophila abortus.
Cathelicidins are antimicrobial peptides, stored by mammalian leukocytes, showing an antimicrobial activity against bacteria, fungi, protozoa and enveloped viruses. In accordance with other authors, we reported in a previous study that the protegrin-1 (PG-1), at 80 microg mL(-1), inhibited the in vitro growth of Chlamydia trachomatis serovars D, H and L2; however, we observed an increased infectivity of some animal chlamydial species after their treatment with the same PG-1 concentration. In this study, the treatment of LLC-MK2 cells with PG-1 before chlamydial infection resulted in an increased infectivity of Chlamydophila abortus probably due to their easier entry into the host cells, whereas no increase in S26/3 infectivity was detected in LLC-MK2 cells treated with PG-1 postchlamydial infection.
Donati
Manuela
M
Policlinico S. Orsola, University of Bologna, Italy.
Di Francesco
Antonietta
A
Gennaro
Renato
R
Benincasa
Monica
M
Di Paolo
Maria
M
Shurdhi
Alisa
A
Ostanello
Fabio
F
Baldelli
Raffaella
R
Cevenini
Roberto
R
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002460:Cell Line; D023501:Chlamydophila / Q000187:drug effects* / Q000472:pathogenicity*; D002479:Inclusion Bodies / Q000382:microbiology; D008253:Macaca mulatta
D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; C094880:protegrin-1