title: Tissue and cell tropism of African horse sickness virus demonstrated by immunoperoxidase labeling in natural and experimental infection in horses in South Africa.
abstract: Tissues from 196 experimental and confirmed natural cases of African horse sickness (all 9 serotypes) were examined with a standardized and validated immunohistochemical assay for detection of the causative virus. The study confirmed that heart and lung are the main target tissues for African horse sickness virus (across all serotypes), followed closely by spleen. It also indicated that microvascular endothelial cells and monocyte-macrophages are the main target cells for virus replication. The importance of monocytes as target cells was emphasized, with relatively few tissue macrophages containing antigen in the lung and spleen, respectively. The results were largely in agreement with those of previous studies, but the large number of cases examined permitted more precise description of the location and distribution of antigen in different tissues. Comparison with descriptions of tissue and cell tropism of other orbiviruses indicated similarity with African horse sickness. Immunohistochemistry was shown to be a useful and consistent technique for demonstrating target cells, but the difficulty of identifying cell types-in particular, different types of monocyte-macrophages-is a limitation.
authors.affiliation: PO Box 14196, Hatfield, 0028, Pretoria, South Africa. sarah.clift@up.ac.za
authors.forename: S J
authors.initials: SJ
authors.lastname: Clift
authors.forename: M-L
authors.initials: ML
authors.lastname: Penrith
mesh_terms: D000355:African Horse Sickness / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D000356:African Horse Sickness Virus / Q000302:isolation & purification*; D000818:Animals; D006321:Heart / Q000821:virology*; D006736:Horses; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D008168:Lung / Q000821:virology*; D012189:Retrospective Studies; D013154:Spleen / Q000821:virology*