Climbing to the next step: photosensitive protein labels.
The tool set for intracellular labeling has received its newest addition. It is now possible to attach a fluorescent molecule to proteins inside living cells and to change the fluorescent properties at some point in time by a flash of light. Hence the labeled protein is easily and unambiguously trackable against a background of nonilluminated proteins from different locations.
Schultz
Carsten
C
European Molecular Biology Laboratory, Heidelberg, Germany. schultz@embl.de <schultz@embl.de>
D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D042541:Intracellular Space / Q000737:chemistry; D008856:Microscopy, Fluorescence; D055668:Photochemical Processes; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D011506:Proteins; D011993:Recombinant Fusion Proteins