Identification of a calcium binding protein highly localized to the cochlear nucleus.
A protein, designated as protein 10 (MW = 29 kDa, pI = 5.3), was shown to be highly localized to the cochlear nuclei of guinea-pigs and rats by the use of two-dimensional gel electrophoresis. In gels from guinea-pig brain, this protein appeared greatest in amounts in the ventral cochlear nucleus and within the deep regions of the dorsal nucleus. Intermediate amounts of protein 10 were found in the lateral superior olivary nucleus and dorsal nucleus of the lateral lemniscus with lesser amounts in telencephalic brain regions. Finally, incubation of nitrocellulose blots in (45)Ca(2+) revealed that protein 10 is a calcium binding protein.
Winsky
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Laboratory of Clinical Science, National Institute of Mental Health, Bldg 10, Rm 3D-48, Bethesda, MD 20892, U.S.A.
Nakata
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Jacobowitz
D M
DM