Antithrombin-III attenuates hepatocyte apoptosis in bile duct ligated rat: a striking cellular change.
OBJECTIVE
Retention and accumulation of toxic hydrophobic bile salts within hepatocyte may cause hepatocyte toxicity by inducing apoptosis. This study was designed with the purpose of evaluating the possible effect of antithrombin-III on hepatocyte apoptosis in bile duct ligated rat.


METHODS
The rats were randomized to 3 groups: group 1 (control, C) underwent sham operation; group 2 (obstructive jaundice, OB) underwent common bile duct ligation; and group 3 (obstructive jaundice with antithrombin-III, OBAT-III) underwent common bile duct ligation and simultaneously were treated with antithrombin-III. Liver tissues were harvested on the fifth postoperative day.


RESULTS
Hepatocyte apoptosis was significantly increased in bile duct ligated group when compared with the sham operation group. The administration of antithrombin-III effectively attenuates such phenomenon in obstructive jaundice with antithrombin-III group.


CONCLUSIONS
Bile duct ligation significantly increased hepatocyte apoptosis and the administration of antithrombin-III effectively attenuates such phenomenon.
Huang
Chun-Ying
CY
Dept. of Surgery, Chang Gung Memorial Hospital, Kaohsiung Medical Center, College of Medicine, Chang Gung University, Taiwan.
Sheen-Chen
Shyr-Ming
SM
Ho
Hsin-Tsung
HT
Tang
Rei-Ping
RP
Eng
Hock-Liew
HL
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000990:Antithrombin III / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D003135:Common Bile Duct / Q000601:surgery; D004195:Disease Models, Animal; D022781:Hepatocytes / Q000187:drug effects*; D041781:Jaundice, Obstructive / Q000503:physiopathology*; D008026:Ligation; D008099:Liver / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000893:Anti-Inflammatory Agents; D000990:Antithrombin III