title: Deletion of btn1, an orthologue of CLN3, increases glycolysis and perturbs amino acid metabolism in the fission yeast model of Batten disease.
abstract: The neuronal ceroid lipofuscinoses (NCLs) constitute a group of autosomal recessive neurodegenerative diseases affecting children. To date, the disease pathogenesis remains unknown, although the role of lysosomal impairment is widely recognized across the different diseases. Recently, the creation of simple models of juvenile NCL (Batten disease) has provided additional insights into the disease mechanism at the molecular level. We report defects in metabolism identified in the Schizosacchromyces pombe yeast model, where btn1, the orthologue of CLN3, has been deleted, using a metabolomics approach based on high resolution 1H and 13C NMR spectroscopy. Such changes represent the first documented metabolic changes associated with deletion of btn1. A decrease in extracellular glucose and increases in the concentration of extracellular ethanol and alanine labelling demonstrate increased glycolytic flux that may arise from vacuolar impairment, whilst amino acid changes were detected which were also in accordance with defective vacuolar functionality. That these changes were detected using a metabolomic based approach advocates its use to further analyse other yeast models of human disease to better understand the function of orthologue genes.
authors.affiliation: Department of Biochemistry, University of Cambridge, Hopkins Building, Tennis Court Road, Cambridge CB21QW, UK.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Pears
authors.forename: Sandra
authors.initials: S
authors.lastname: Codlin
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Haines
authors.forename: Ian J
authors.initials: IJ
authors.lastname: White
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Mortishire-Smith
authors.forename: Sara E
authors.initials: SE
authors.lastname: Mole
authors.forename: Julian L
authors.initials: JL
authors.lastname: Griffin
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002648:Child; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure; D017353:Gene Deletion; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism; D006019:Glycolysis; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D055432:Metabolomics; D046529:Microscopy, Electron, Transmission; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D009472:Neuronal Ceroid-Lipofuscinoses / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D013395:Sucrose / Q000494:pharmacology
chemical_list: D000596:Amino Acids; C509077:BTN1 protein, S pombe; C105199:CLN3 protein, human; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D029702:Schizosaccharomyces pombe Proteins; D013395:Sucrose; D006003:Glycogen; D005947:Glucose; D005990:Glycerol