The plasminogen system in microdissected colonic mucosa distant from an isolated adenoma.
In the colon, the urokinase-type plasminogen activator (uPA), its receptor (uPAR), and plasminogen activator inhibitors, PAI-1 and PAI-2, are implicated in the transition from mucosa to adenoma and tumour progression. However, expression in the mucosa adjacent, or distant, to an adenoma has not yet been investigated. Three biopsies from mucosae adjacent (20 cm, ipsilateral) and distant (contralateral) to an isolated tubular adenoma were analysed in 14 patients and 8 controls. Laser microdissection isolated stromal and epithelial crypt components, and quantitative RT-PCR analyses of uPA, uPAR, PAI-1 and PAI-2 mRNA levels were performed. Among controls, no significant differences in the markers were noted. With left colon isolated tubular adenoma, uPA, uPAR, and PAI-2 mRNA levels were significantly increased in the adjacent mucosal stroma compared to epithelial crypt levels (p < 0.05). In right colon adenoma, the mRNA levels of these 3 molecular markers were significantly increased only in the adjacent mucosal stromal samples (p < 0.05). Isolated tubular adenoma in the colon increases significantly the mRNA levels of 3 proteolysis-associated molecular markers in the stromal, but not in the epithelial, components of adjacent mucosa. These results suggest the presence of regional and dynamic interactions in apparently non-involved mucosae.
Saucy
Francois
F
Division of Gastroenterology and Hepatology, University Hospital Lausanne, Lausanne 1011, Switzerland. francois.saucy@chuv.ch
Bachmann
Daniel
D
Peterman
Olivier
O
Sordat
Bernard
B
Sordat
Isabelle
I
Dorta
Gian
G
D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D000368:Aged; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007834:Lasers; D008297:Male; D042282:Microdissection; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis*; D017396:Plasminogen Activator Inhibitor 2 / Q000096:biosynthesis*; D010960:Plasminogen Activators / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D055293:Receptors, Urokinase Plasminogen Activator / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017395:Plasminogen Activator Inhibitor 1; D017396:Plasminogen Activator Inhibitor 2; D012333:RNA, Messenger; D055293:Receptors, Urokinase Plasminogen Activator; D010960:Plasminogen Activators