title: MicroRNA miR-137 regulates neuronal maturation by targeting ubiquitin ligase mind bomb-1.
abstract: The maturation of young neurons is regulated by complex mechanisms and dysregulation of this process is frequently found in neurodevepmental disorders. MicroRNAs have been implicated in several steps of neuronal maturation including dendritic and axonal growth, spine development, and synaptogenesis. We demonstrate that one brain-enriched microRNA, miR-137, has a significant role in regulating neuronal maturation. Overexpression of miR-137 inhibits dendritic morphogenesis, phenotypic maturation, and spine development both in brain and cultured primary neurons. On the other hand, a reduction in miR-137 had opposite effects. We further show that miR-137 targets the Mind bomb one (Mib1) protein through the conserved target site located in the 3' untranslated region of Mib1 messenger RNA. Mib1 is an ubiquitin ligase known to be important for neurodevelopment. We show that exogenously expressed Mib1 could partially rescue the phenotypes associated with miR-137 overexpression. These results demonstrate a novel miRNA-mediated mechanism involving miR-137 and Mib1 that function to regulate neuronal maturation and dendritic morphogenesis during development.
authors.affiliation: Department of Neurosciences, University of New Mexico School of Medicine, Albuquerque, New Mexico, USA.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smrt
authors.forename: Keith E
authors.initials: KE
authors.lastname: Szulwach
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Pfeiffer
authors.forename: Xuekun
authors.initials: X
authors.lastname: Li
authors.forename: Weixiang
authors.initials: W
authors.lastname: Guo
authors.forename: Manavendra
authors.initials: M
authors.lastname: Pathania
authors.forename: Zhao-Qian
authors.initials: ZQ
authors.lastname: Teng
authors.forename: Yuping
authors.initials: Y
authors.lastname: Luo
authors.forename: Junmin
authors.initials: J
authors.lastname: Peng
authors.forename: Angelique
authors.initials: A
authors.lastname: Bordey
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D010641:Phenotype; D014176:Protein Biosynthesis; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: C551799:MIRN137 microRNA, mouse; D035683:MicroRNAs; C470629:MIB1 protein, mouse; D044767:Ubiquitin-Protein Ligases