title: [The biological effect of minocycline on human periodontal ligament fibroblast].
abstract: OBJECTIVE
  To investigate the effective concentration range of minocycline as a local drug delivery system.
  
  
  METHODS
  A pilot study of minocycline on proliferation,protein synthesis,collagen I synthesis,ALP activity,osteocalcin secretion of human periodontal ligament fibroblast(hPDLF) was carried out. Different concentrations of minocycline(0,10,20,40,100,200mg/L) were added to the fifth passage(P5) hPDLF. After 1,4,7,10,14 days of co-incubation,the proliferation activity was measured with MTT assay; Protein synthesis, collagen I synthesis, ALP activity and osteocalcin secretion were measured. Statistical analysis was performed with SPSS 13.0 software package.
  
  
  RESULTS
  10-200mg/L minocycline couLd significantly increase the proliferation of P5 hPDLF(P<0.05), and 100mg/L minocycline was the optimal effect. 20-200mg/L minocycline could significantly increase protein synthesis and collagen I synthesis(P<0.05), 10mg/L could significantly increase ALP activity(P<0.05), 20-200mg/L could significantly inhibit ALP activity (P<0.05); 40-200mg/L could significantly inhibit osteocalcin secretion(P<0.05). 10-20mg/L minocycline increased proliferation, protein synthesis, collagen I synthesis, ALP activity, osteocalcin secretion of P5 hPDLF, but did not inhibit osteocalcin secretion.
  
  
  CONCLUSIONS
  10-20mg/L might be the effective concentration range of minocycline as a local drug delivery system. Supported by National Natural Science Foundation of China(Grant No.30973354).
authors.affiliation: Department of Prosthodontics, The 401st Hospital of Chinese PLA, Qingdao 266071, Shandong Province, China. tiantianmama9288@126.com
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong-chen
authors.initials: HC
authors.lastname: Liu
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: E
authors.forename: Shi-jun
authors.initials: SJ
authors.lastname: Li
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhao
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Wang
mesh_terms: D002478:Cells, Cultured; D005347:Fibroblasts; D006801:Humans; D008911:Minocycline; D010513:Periodontal Ligament; D010865:Pilot Projects
chemical_list: D008911:Minocycline