A cyclophilin A CPR1 overexpression enhances stress acquisition in Saccharomyces cerevisiae.
Cyclophilins are conserved cis-trans peptidyl-prolyl isomerase that are implicated in protein folding and function as molecular chaperones. We found the expression of cyclophilin A, Cpr1, changes in response to exposure to yeast Saccharomyces cerevisiae to abiotic stress conditions. The effect of Cpr1 overexpression in stress responses was therefore examined. The CPR1 gene was cloned to the yeast expression vector pVTU260 under regulation of an endogenous alcohol dehydrogenase (ADH) promoter. The overexpression of Cpr1 drastically increased cell viability of yeast in the presence of stress inducers, such as cadmium, cobalt, copper, hydrogen peroxide, tert-butyl hydroperoxide (t-BOOH), and sodium dodecyl sulfate (SDS). The Cpr1 expression also enhanced the cell rescue program resulting in a variety of antioxidant enzymes including thioredoxin system (particularly, thioredoxin peroxidase), metabolic enzymes (glucose-6-phosphate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase), and molecular chaperones (Hsp104, Hsp90, Hsp60 and Hsp42). Thus, our study illustrates the importance of Cpr1 as a molecular chaperone that improves cellular stress responses through collaborative relationships with other proteins when yeast cells are exposed to adverse conditions, and it also premises the improvement of yeast strains.
Kim
Il-Sup
IS
Department of Biology, Kyungpook National University, Daegu, 702-701, Korea.
Kim
Hyun-Young
HY
Shin
Sun-Young
SY
Kim
Young-Saeng
YS
Lee
Dong Hee
DH
Park
Kyung Moc
KM
Yoon
Ho-Sung
HS
D002470:Cell Survival / Q000235:genetics; D003001:Cloning, Molecular; D021984:Cyclophilin A / Q000235:genetics / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D008670:Metals / Q000378:metabolism; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D019076:Transgenes / Q000235:genetics; D015854:Up-Regulation; D020122:tert-Butylhydroperoxide / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D008670:Metals; C083756:SSE2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012967:Sodium Dodecyl Sulfate; D020122:tert-Butylhydroperoxide; D005954:Glucosephosphate Dehydrogenase; D054464:Peroxiredoxins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D021984:Cyclophilin A