Transgenic expression of trehalulose synthase results in high concentrations of the sucrose isomer trehalulose in mature stems of field-grown sugarcane.
Sugarcane plants were developed that produce the sucrose isomers trehalulose and isomaltulose through expression of a vacuole-targeted trehalulose synthase modified from the gene in 'Pseudomonas mesoacidophila MX-45' and controlled by the maize ubiquitin (Ubi-1) promoter. Trehalulose concentration in juice increased with internode maturity, reaching about 600 mM, with near-complete conversion of sucrose in the most mature internodes. Plants remained vigorous, and trehalulose production in selected lines was retained over multiple vegetative generations under glasshouse and field conditions.
Hamerli
Dénes
D
Botany Department/BIOL, The University of Queensland, Brisbane, Qld, Australia.
Birch
Robert G
RG
D004187:Disaccharides / Q000096:biosynthesis*; D019751:Intramolecular Transferases / Q000235:genetics* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000235:genetics; D031786:Saccharum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D025822:Ubiquitin C / Q000235:genetics / Q000378:metabolism; D014617:Vacuoles / Q000201:enzymology; D003313:Zea mays / Q000235:genetics
D004187:Disaccharides; D010940:Plant Proteins; C417884:Ubi-1 protein, Zea mays; D025822:Ubiquitin C; D019751:Intramolecular Transferases; C042002:isomaltulose synthase; C042003:trehalulose