title: Visible fluorescence detection of type III protein secretion from bacterial pathogens.
abstract: Type III protein secretion is essential for many gram-negative bacterial infections of host cells and an attractive target for new antibacterial drugs. Here, we describe a bacterial protein effector-carboxypeptidase G2 (CPG2) reporter system for fluorescence and visible detection of type III protein secretion in Salmonella typhimurium. This system provides a general method for measuring protein expression and secretion as well as a high-throughput and quantitative assay for analyzing type III protein secretion inhibitors.
authors.affiliation: Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065, USA.
authors.forename: Jacob S
authors.initials: JS
authors.lastname: Yount
authors.forename: Lun K
authors.initials: LK
authors.lastname: Tsou
authors.forename: Paul D
authors.initials: PD
authors.lastname: Dossa
authors.forename: Amy L
authors.initials: AL
authors.lastname: Kullas
authors.forename: Adrianus W M
authors.initials: AW
authors.lastname: van der Velden
authors.forename: Howard C
authors.initials: HC
authors.lastname: Hang
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D003116:Color; D006367:HeLa Cells; D006801:Humans; D019779:Salmonella enterica; D013050:Spectrometry, Fluorescence / Q000379:methods*; D011623:gamma-Glutamyl Hydrolase / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011623:gamma-Glutamyl Hydrolase