title: TRPV1 acts as a synaptic protein and regulates vesicle recycling.
abstract: Electrophysiological studies demonstrate that transient receptor potential vanilloid subtype 1 (TRPV1) is involved in neuronal transmission. Although it is expressed in the peripheral as well as the central nervous system, the questions remain whether TRPV1 is present in synaptic structures and whether it is involved in synaptic processes. In the present study we gathered evidence that TRPV1 can be detected in spines of cortical neurons, that it colocalizes with both pre- and postsynaptic proteins, and that it regulates spine morphology. Moreover, TRPV1 is also present in biochemically prepared synaptosomes endogenously. In F11 cells, a cell line derived from dorsal-root-ganglion neurons, TRPV1 is enriched in the tips of elongated filopodia and also at sites of cell-cell contact. In addition, we also detected TRPV1 in synaptic transport vesicles, and in transport packets within filopodia and neurites. Using FM4-64 dye, we demonstrate that recycling and/or fusion of these vesicles can be rapidly modulated by TRPV1 activation, leading to rapid reorganization of filopodial structure. These data suggest that TRPV1 is involved in processes such as neuronal network formation, synapse modulation and release of synaptic transmitters.
authors.affiliation: Signal Transduction in Pain and Mental Retardation, Department for Molecular Human Genetics, Max-Planck Institute for Molecular Genetics, Berlin, Germany. chandan@niser.ac.in
authors.forename: Chandan
authors.initials: C
authors.lastname: Goswami
authors.forename: Nils
authors.initials: N
authors.lastname: Rademacher
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Smalla
authors.forename: Vera
authors.initials: V
authors.lastname: Kalscheuer
authors.forename: Hans-Hilger
authors.initials: HH
authors.lastname: Ropers
authors.forename: Eckart D
authors.initials: ED
authors.lastname: Gundelfinger
authors.forename: Tim
authors.initials: T
authors.lastname: Hucho
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D051379:Mice; D016501:Neurites / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D021381:Protein Transport; D011554:Pseudopodia / Q000235:genetics / Q000378:metabolism; D013569:Synapses / Q000235:genetics / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*; D022161:Transport Vesicles / Q000378:metabolism*
chemical_list: D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse