title: Proteomic adaptation to chronic high intensity swimming training in the rat heart.
abstract: Cardiac hypertrophy induced by exercise is associated with less cardiac fibrosis and better systolic and diastolic function, suggesting that the adaptive mechanisms may exist in exercise-induced hypertrophy. To identify molecular mechanisms by which exercise training stimulates this favorable phenotype, a proteomic approach was employed to detect rat cardiac proteins that were differentially expressed or modified after exercise training. Sixteen male Sprague-Dawley rats were divided into trained (T) and control(C). T rats underwent eight weeks of swimming training seven days/week, using a high intensity protocol. Hearts were used to generate 2-D electrophoretic proteome maps. Training significantly altered 23 protein spot intensities (P<0.05), including proteins associated with the mitochondria oxidative metabolism, such as prohibitin, malate dehydrogenase, short-chain acyl-CoA dehydrogenase, triosephosphate isomerase, electron transfer flavoprotein subunit beta, ndufa10 protein, ATP synthase subunit alpha and isocitrate dehydrogenase [NAD] subunit. Additionally, Prohibitin was increased in the exercise-induced hearts. Cytoskeletal, signal pathway, stress and oxidative proteins also increased within T groups. These results strongly support the notion that the observed changes in the expression of energy metabolism proteins resulted in a potential increase in the capacity to synthesise ATP, probably via mitochondrial oxidative metabolism. The observed changes in the expression of these metabolic and structural proteins induced by training may beneficially influence heart metabolism, stress response and signalling paths, and therefore improve the overall cardiac function.
authors.affiliation: Department of Human Sports Science, Nanjing Institute of Physical Education, Nanjing, 210014, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Sun
authors.forename: Jun Hong
authors.initials: JH
authors.lastname: Wang
authors.forename: Yuan Yuan
authors.initials: YY
authors.lastname: Lv
authors.forename: Shu Shu
authors.initials: SS
authors.lastname: Zhu
authors.forename: Jin
authors.initials: J
authors.lastname: Yang
authors.forename: Ji Zheng
authors.initials: JZ
authors.lastname: Ma