Antiproliferative effect of adiponectin on rat uterine leiomyoma ELT-3 cells.
OBJECTIVE
Although fibroids greatly affect reproductive health, the pathophysiology and epidemiology are not well known. Recently, we have reported the relationship between uterine leiomyoma and metabolic syndrome. Many studies have indicated that reductions in plasma adiponectin levels play major roles in the development of metabolic syndrome. In this study, we investigated the significant repressive effect of adiponectin on rat uterine leiomyoma ELT-3 cells proliferation.


METHODS
Expression of adiponectin receptor 1 and receptor 2 was evaluated by RT-PCR and Western blot analysis. Cell proliferation was assessed by the MTS assay and cell counting. Apoptosis was evaluated by Hoechst staining and cleaved poly (ADP-ribose) polymerase (PARP).


RESULTS
Adiponectin receptor 1 and receptor 2 were expressed in ELT-3 cells. Adiponectin repressed rat uterine leiomyoma ELT-3 cells cell proliferation without inducing apoptosis.


CONCLUSIONS
The repression of adiponectin on leiomyoma cell proliferation in the rat may explain a crucial role of adiponectin in the association of metabolic syndrome with uterine leiomyoma.
Wakabayashi
Atsuko
A
Department of Obstetrics and Gynecology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
Takeda
Takashi
T
Tsuiji
Kenji
K
Li
Bin
B
Sakata
Masahiro
M
Morishige
Ken-ichirou
K
Yaegashi
Nobuo
N
Kimura
Tadashi
T
D052242:Adiponectin / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007889:Leiomyoma / Q000737:chemistry / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D054419:Receptors, Adiponectin / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014594:Uterine Neoplasms / Q000737:chemistry / Q000473:pathology*
D052242:Adiponectin; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; C504339:adiponectin receptor 1, rat; C506573:adiponectin receptor 2, rat