On the disposition of phospholipids in freshly isolated myelin sheath preparations from bovine brain.
The extent of availability of the four major classes of phospholipids in freshly isolated bovine brain myelin has been studied using phospholipase C (Clostridium perfringens), phospholipase A (bee venom), sphingomyelase (Staphalococcus aureus) and trinitrobenzenesulphonate. The results have been compared with those of a previous study (Gwarsha et al., 1984). Taken together, the data suggest that 47-67% of the phosphatidylcholine, 45-66% of the ethanolamine-containing phosphoglycerides, 52-55% of the spingomyelin and 10-32% of the phosphatidylserine + phosphatidylinositol in the fresh myelin is available to these probes and hence may be located at the external surface of the membrane bilayer.
Gwarsha
K
K
Department of Biology, University of York, Heslington, York Y01 5DD, U.K.
Rumsby
M G
MG
Little
C
C