title: Identifying drug metallation sites on peptides using electron transfer dissociation (ETD), collision induced dissociation (CID) and ion mobility-mass spectrometry (IM-MS).
abstract: Electron transfer dissociation (ETD) and collision induced dissociation (CID) have been used to locate the precise binding sites for platinum and ruthenium anticancer complexes on the peptide Substance P. We show that ETD combined with ion mobility-mass spectrometry significantly reduces mass spectral complexity and improves the S/N of the product-ions formed.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, UK CV4 7AL.
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Williams
authors.forename: Jeffery M
authors.initials: JM
authors.lastname: Brown
authors.forename: Iain
authors.initials: I
authors.lastname: Campuzano
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Sadler
mesh_terms: D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites; D017671:Platinum Compounds / Q000737:chemistry / Q000494:pharmacology*; D017975:Ruthenium Compounds / Q000737:chemistry / Q000494:pharmacology*; D013373:Substance P / Q000737:chemistry / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D017671:Platinum Compounds; D017975:Ruthenium Compounds; D013373:Substance P