An experimental model for the study of craniofacial deformities.
OBJECTIVE
To develop an experimental surgical model in rats for the study of craniofacial abnormalities.


METHODS
Full thickness calvarial defects with 10x10-mm and 5x8-mm dimensions were created in 40 male NIS Wistar rats, body weight ranging from 320 to 420 g. The animals were equally divided into two groups. The periosteum was removed and dura mater was left intact. Animals were killed at 8 and 16 weeks postoperatively and cranial tissue samples were taken from the defects for histological analysis.


RESULTS
Cranial defects remained open even after 16 weeks postoperatively.


CONCLUSIONS
The experimental model with 5x8-mm defects in the parietal region with the removal of the periosteum and maintenance of the integrity of the dura mater are critical and might be used for the study of cranial bone defects in craniofacial abnormalities.
Costa
André de Mendonça
Ade M
University of Health Sciences, Alagoas, Brazil. andredemendonca@usp.br <andredemendonca@usp.br>
Kobayashi
Gerson Shigeru
GS
Bueno
Daniela Franco
DF
Martins
Marília Trierveiler
MT
Ferreira
Marcus de Castro
Mde C
Passos-Bueno
Maria Rita
MR
Alonso
Nivaldo
N
D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D004388:Dura Mater / Q000473:pathology; D008297:Male; D023421:Models, Animal; D010027:Osteotomy; D010294:Parietal Bone / Q000293:injuries / Q000601:surgery*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014945:Wound Healing