Transposon-based screens for cancer gene discovery in mouse models.
Significant emphasis has recently been placed on the characterization of the human cancer genome. This effort has been assisted by the development of new DNA sequencing technologies that allow the genomes of individual tumors to be analyzed in much greater detail. However, the genetic complexity of human cancer has complicated the identification of driver mutations among the more abundant passenger mutations found in tumors. Recently, the Sleeping Beauty (SB) transposon system has been engineered to model cancer in mice. SB-induced tumors are produced by transposon insertional mutagenesis, thus the tagged mutations facilitate the identification of novel cancer genes. This review provides a brief summary of the SB system and its use in modeling cancer in mice.
Dupuy
Adam J
AJ
Department of Anatomy & Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, United States. adam-dupuy@uiowa.edu
D000818:Animals; D004251:DNA Transposable Elements / Q000502:physiology*; D004195:Disease Models, Animal; D052138:Genes, Neoplasm; D056726:Genetic Association Studies / Q000379:methods*; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D051379:Mice / Q000235:genetics*; D008954:Models, Biological; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D019895:Transposases / Q000378:metabolism / Q000502:physiology
D004251:DNA Transposable Elements; D019895:Transposases; C494471:sleeping beauty transposase, human