Obesity and polycystic ovary syndrome: association with androgens, leptin and its genotypes.
Obesity and hyperandrogenaemia are key features of polycystic ovary syndrome (PCOS). The aim of this study was to investigate whether leptin and androgens are associated with obesity in PCOS subjects and identify whether there exist any genetic alterations in leptin gene in women with PCOS. The results reveal that leptin levels are elevated in women with PCOS and associate with BMI. However, irrespective of the obesity status leptin levels are higher in PCOS cases indicating that increased BMI/obesity may not be the only factor contributing to elevated levels of leptin. With regard to testosterone and androstenedione, the levels were increased in obese individuals irrespective of PCOS status. No correlation between leptin and androstenedione or testosterone was observed in controls and PCOS subjects. The single-nucleotide polymorphism G19A detected in the untranslated exon 1 of leptin gene was not associated with PCOS and does not contribute to elevated levels of leptin. The results overall suggest that androgen and leptin levels are increased in PCOS and obesity. It demonstrates that obesity is a confounding factor for hyperandrogenaemia irrespective of their PCOS status. The study rules out role of obesity status and leptin genotype in increase in leptin levels observed in PCOS cases.
Pusalkar
Madhavi
M
Department of Molecular Endocrinology, National Institute for Research in Reproductive Health (ICMR), Parel, Mumbai, India.
Meherji
Pervin
P
Gokral
Jyotsna
J
Savardekar
Lalita
L
Chinnaraj
Saravanan
S
Maitra
Anurupa
A
D000328:Adult; D000735:Androstenedione / Q000097:blood*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D020738:Leptin / Q000097:blood* / Q000235:genetics; D016014:Linear Models; D009765:Obesity / Q000097:blood* / Q000235:genetics; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000235:genetics; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D013739:Testosterone / Q000097:blood*
D020738:Leptin; D013739:Testosterone; D000735:Androstenedione; D004247:DNA