title: Porcine NAMPT gene: search for polymorphism, mapping and association studies.
abstract: NAMPT encodes an enzyme catalysing the rate-limiting step in NAD biosynthesis. The extracellular form of the enzyme is known as adipokine visfatin. We detected SNP AM999341:g.669T>C (referred to as 669T>C) in intron 9 and SNP FN392209:g.358A>G (referred to as 358A>G) in the promoter of the gene. RH mapping linked the gene to microsatellite SW944. Linkage analysis placed the gene on the current USDA – USMARC linkage map at position 92 cM on SSC9. Association analyses were performed in a wild boar × Meishan F2 family (W × M), with 45 traits recorded (growth and fattening, fat deposition, muscling, meat quality, stress resistance and other traits), and in a commercial Landrace × Chinese-European (LCE) synthetic population with records for 15 traits (growth, fat deposition, muscling, intramuscular fat, meat colour and backfat fatty acid content). In the W × M, SNP 669T>C was associated with muscling, fat deposition, growth and fattening, meat quality and other traits and in the LCE with muscling, meat quality and backfat fatty acid composition. In the W × M, SNP 358A>G was associated with muscling, fat deposition, growth and other traits. After correction for multiple testing, the NAMPT haplotypes were associated in the W × M with, in descending order, muscling (q = 0.0056), growth (q = 0.0056), fat deposition (q = 0.0109), fat-to-meat ratio (q = 0.0135), cooling losses (q = 0.0568) and longissimus pHU (q = 0.0695). The SNPs are hypothesized to be in linkage disequilibrium with a causative mutation affecting energy metabolism as a whole rather than fat metabolism alone.
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic. cepica@iapg.cas.cz
authors.forename: S
authors.initials: S
authors.lastname: Cepica
authors.forename: H
authors.initials: H
authors.lastname: Bartenschlager
authors.forename: C
authors.initials: C
authors.lastname: Ovilo
authors.forename: J
authors.initials: J
authors.lastname: Zrůstová
authors.forename: M
authors.initials: M
authors.lastname: Masopust
authors.forename: A
authors.initials: A
authors.lastname: Fernández
authors.forename: A
authors.initials: A
authors.lastname: López
authors.forename: A
authors.initials: A
authors.lastname: Knoll
authors.forename: G A
authors.initials: GA
authors.lastname: Rohrer
authors.forename: W M
authors.initials: WM
authors.lastname: Snelling
authors.forename: H
authors.initials: H
authors.lastname: Geldermann
mesh_terms: D000818:Animals; D050218:Body Fat Distribution; D002874:Chromosome Mapping; D004734:Energy Metabolism; D005260:Female; D056726:Genetic Association Studies / Q000379:methods*; D006239:Haplotypes / Q000235:genetics; D007438:Introns / Q000235:genetics; D015810:Linkage Disequilibrium / Q000235:genetics*; D008297:Male; D008460:Meat / Q000592:standards; D018895:Microsatellite Repeats; D024510:Muscle Development; D054409:Nicotinamide Phosphoribosyltransferase / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development
chemical_list: D054409:Nicotinamide Phosphoribosyltransferase