Synthesis of the Cyanine 7 labeled neutrophil-specific agents for noninvasive near infrared fluorescence imaging.
A neutrophil-binding peptide, cinnamoyl-F(D)LF(D)LF (cFLFLF), was labeled with the near infrared (NIR) fluorophore, Cyanine 7 (Cy7). This construct was modified with a polyethylene glycol (PEG, M(W) 3.4 kDa) moiety in order to increase its solubility and bioavailability to circulating neutrophils. A preliminary noninvasive fluorescence imaging of a mouse model of ear inflammation with the fluorescent probe is presented.
Xiao
Li
L
Department of Radiology, The University of Virginia, Charlottesville, VA 22908, USA.
Zhang
Yi
Y
Liu
Zhongqiu
Z
Yang
Min
M
Pu
Lin
L
Pan
Dongfeng
D
D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry; D001682:Biological Availability; D002232:Carbocyanines / Q000737:chemistry; D003952:Diagnostic Imaging / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007249:Inflammation / Q000175:diagnosis; D007259:Infrared Rays; D051379:Mice; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology*; D011092:Polyethylene Glycols; D012995:Solubility
D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D011092:Polyethylene Glycols; C011204:cyanine dye 7