Anti-obesity effect of an isoflavone fatty acid ester on obese mice induced by high fat diet and its potential mechanism.
BACKGROUND
The novel compound 1a is one of the isoflavone fatty acid esters. In order to investigate the anti-obesity effect of compound 1a and its potential mechanism of influence in adipocyte differentiation, Obese male C57BL/6J mice induced by high-fat diet (HFD) and rat preadipocytes (3T3-L1 cell) were used.


METHODS
After 4-week HFD induction, the obese model was made successfully. After treatment with compound 1a, mice plasma biochemistry parameters were analyzed. In addition, mice hepatic tissue slice was observed. In in vitro research, 3T3-L1 cell differentiation by Oil-Red-O staining and adipocyte apoptosis was detected by flow cytometry.


RESULTS
The in vivo results implied that compound 1a significantly decreased the body weight, white adipose tissue weight of obesity mice (p<0.05), reduced leptin and TG in plasma (p<0.05), elevated HDL-C in serum (p<0.05). The in vitro results suggested that compound 1a could significantly suppress the adipocyte viability and lipid accumulation in the differentiation of preadipocyte, and induce apoptosis in both preadipocytes and mature adipocytes (p<0.05).


CONCLUSIONS
Compound 1a regulates serum lipid profiles, decreases adipose tissue mass and body weight gain by inducing adipocyte apoptosis in high fat diet induced mice. Thus, it may be used to treat obese patients with hypercholesterolemia and hypertriglyceridemia.
Yao
Yao
Y
Nanjing Brain Hospital affiliated to Nanjing Medical University, Nanjing 210029, PR China.
Li
Xiao-Bo
XB
Zhao
Wei
W
Zeng
Yan-Yan
YY
Shen
Hong
H
Xiang
Hua
H
Xiao
Hong
H
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology; D050154:Adiposity / Q000187:drug effects*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004041:Dietary Fats / Q000008:administration & dosage; D007529:Isoflavones / Q000494:pharmacology* / Q000627:therapeutic use; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000188:drug therapy* / Q000209:etiology
D019440:Anti-Obesity Agents; D004041:Dietary Fats; D007529:Isoflavones