DOX-loaded PEG-PLGA and Pluronic copolymer composite micelles enhances cytotoxicity and the intracellular accumulation of drug in DOX-resistant tumor cells.
In the present study, doxorubicin (DOX) loaded polyethyleneglycol-poly (DL-lactic-co-glycolic acid) micelle as well as composite micelles composed polyethyleneglycol- poly(DL-lactic-co-glycolic acid) (PEG-PLGA) and Pluronic 105 (P105) were constructed. The micelles, with diameter around 106 nm and 85 nm respectively, were prepared by solvent evaporation method. The results showed that the encapsulation of DOX in micelles could significantly enhance its cytotoxicity in a DOX resistant tumor cell line, K562/DOX. The combination of PEG-PLGA and Pluronic further improved both the tumor-suppressive activity and the intracellular accumulation of DOX, indicating that the composite micelles would be potential to reverse the multidrug resistance in tumor cells.
Diao
Yuan-Yuan
YY
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, P.R. China.
Han
Min
M
Ding
Ping-Tian
PT
Chen
Da-Wei
DW
Gao
Jian-Qing
JQ
D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D020442:Poloxamer; D011092:Polyethylene Glycols; D011098:Polyglactin 910; D013778:Tetrazolium Salts; D013844:Thiazoles
D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D008823:Micelles; D013778:Tetrazolium Salts; D013844:Thiazoles; C102418:poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer; D020442:Poloxamer; D011098:Polyglactin 910; D011092:Polyethylene Glycols; D004317:Doxorubicin; C022616:thiazolyl blue