title: The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard.
abstract: The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.
authors.affiliation: Centre for Biodiscovery and School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Peng
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Kapp
authors.forename: David
authors.initials: D
authors.lastname: Fenyö
authors.forename: Min-Seok
authors.initials: MS
authors.lastname: Kwon
authors.forename: Pu
authors.initials: P
authors.lastname: Jiang
authors.forename: Songfeng
authors.initials: S
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Marie-Isabel
authors.initials: MI
authors.lastname: Aguilar
authors.forename: Nikhat
authors.initials: N
authors.lastname: Ahmed
authors.forename: Mark S
authors.initials: MS
authors.lastname: Baker
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
authors.forename: Yu-Ju
authors.initials: YJ
authors.lastname: Chen
authors.forename: Phan
authors.initials: P
authors.lastname: Van Chi
authors.forename: Maxey C M
authors.initials: MC
authors.lastname: Chung
authors.forename: Fuchu
authors.initials: F
authors.lastname: He
authors.forename: Alice C L
authors.initials: AC
authors.lastname: Len
authors.forename: Pao-Chi
authors.initials: PC
authors.lastname: Liao
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Nakamura
authors.forename: Sai Ming
authors.initials: SM
authors.lastname: Ngai
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Paik
authors.forename: Tai-Long
authors.initials: TL
authors.lastname: Pan
authors.forename: Terence C W
authors.initials: TC
authors.lastname: Poon
authors.forename: Ghasem Hosseini
authors.initials: GH
authors.lastname: Salekdeh
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Simpson
authors.forename: Ravi
authors.initials: R
authors.lastname: Sirdeshmukh
authors.forename: Chantragan
authors.initials: C
authors.lastname: Srisomsap
authors.forename: Jisnuson
authors.initials: J
authors.lastname: Svasti
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Tyan
authors.forename: Florian S
authors.initials: FS
authors.lastname: Dreyer
authors.forename: Danyl
authors.initials: D
authors.lastname: McLauchlan
authors.forename: Pisana
authors.initials: P
authors.lastname: Rawson
authors.forename: T William
authors.initials: TW
authors.lastname: Jordan
mesh_terms: D000818:Animals; D001208:Asia; D002254:Carbonates; D002462:Cell Membrane / Q000737:chemistry*; D006801:Humans; D007425:Intracellular Membranes / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry* / Q000592:standards; D051379:Mice; D044349:Oceania; D009938:Organizations; D020543:Proteome; D040901:Proteomics / Q000379:methods / Q000592:standards*
chemical_list: D002254:Carbonates; D008565:Membrane Proteins; D020543:Proteome