title: Effects of short-term overfeeding with fructose, fat and fructose plus fat on plasma and hepatic lipids in healthy men.
abstract: OBJECTIVE
  The present study aimed to assess the effects of excess fat, fructose and fat-plus-fructose intakes on intrahepatocellular lipid (IHCL).
  
  
  METHODS
  Healthy male subjects were studied after an isocaloric diet or a 7-day high-fructose (Fru: +3.5 g fructose/kg fat-free mass/day, +35% energy), high-fat (Fat: +30% energy as saturated-fat) or high-fructose, high-fat diet (FruFat: +3.5 g fructose/kg fat-free mass/day, +30% energy as fat, +65% total energy). IHCL was measured by (1)H magnetic resonance spectroscopy.
  
  
  RESULTS
  All hypercaloric diets increased IHCL (Fru: +16%; Fat: +86%; FruFat: +133%; P<0.05). Very low-density lipoprotein (VLDL) triacylglycerols increased after Fru (+58%; P<0.05), but decreased after Fat (-22%; P<0.05), while no change was observed after FruFat.
  
  
  CONCLUSIONS
  Fat and fructose both increased IHCL, but fructose increased, while fat decreased, VLDL triacylglycerols. However, excess fat and fructose combined had additive effects on IHCL and neutralizing effects on VLDL triglycerides. This suggests that fructose stimulates, while fat inhibits, hepatic VLDL triacylglycerol secretion.
authors.affiliation: Department of Physiology, Faculty of Biology and Medicine, University of Lausanne, 7, rue du Bugnon, 1005 Lausanne, Switzerland.
authors.forename: H
authors.initials: H
authors.lastname: Sobrecases
authors.forename: K-A
authors.initials: KA
authors.lastname: Lê
authors.forename: M
authors.initials: M
authors.lastname: Bortolotti
authors.forename: P
authors.initials: P
authors.lastname: Schneiter
authors.forename: M
authors.initials: M
authors.lastname: Ith
authors.forename: R
authors.initials: R
authors.lastname: Kreis
authors.forename: C
authors.initials: C
authors.lastname: Boesch
authors.forename: L
authors.initials: L
authors.lastname: Tappy
mesh_terms: D000704:Analysis of Variance; D001786:Blood Glucose / Q000378:metabolism; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage* / Q000378:metabolism; D005234:Fatty Liver / Q000378:metabolism*; D005632:Fructose / Q000008:administration & dosage* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006963:Hyperphagia / Q000097:blood / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000097:blood* / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D014280:Triglycerides / Q000097:blood* / Q000378:metabolism; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D008079:Lipoproteins, VLDL; D014280:Triglycerides; D005632:Fructose; D005947:Glucose