Gene expression profiles of the incisor pulp tissue during fluorosis.
OBJECTIVE
To examine gene expression levels within the cells of dental pulp tissue in fluorotic rats and normal subjects and to identify fluoride-susceptible genes.


METHODS
Female wistar rats were given 0.16 (control) or 100 parts per 10(6) fluoride ion in their drinking water. After 3 months, the teeth in the fluoride-exposed animals showed signs of fluorosis. The animals were killed, and RNA was extracted from incisor pulp tissue and pooled into three fluorosis and three control pools. The females were analysed for gene expression profiles using microarray analysis and semi-quantitative reverse transcriptase polymerase chain reaction (Sq-RT-PCR).


RESULTS
Of the 26 962 probe sets, duplicate hybridisation data indicated that 5443 genes were detected as being present by both targets. Two hundred and forty seven genes, that is 4.53% of detected genes, were 1.5-fold or greater differentially expressed after fluoride treatment. Three interesting genes, dentine matrix protein 1, dentine sialophosphoprotein and Cadherin-11, were confirmed as being down-regulated by Sq-RT-PCR.


CONCLUSIONS
The gene expression levels in rat pulp tissue changed during fluorosis. The gene expression profiles between the fluorotic model and the normal group will help to understand the multiple pathogenic mechanisms for the altered mineralization patterns observed in fluorotic dentine.
Wu
Y
Y
State Key Laboratory for Oral Disease, Sichuan University, Sichuan, China.
Hao
Y-Q
YQ
Li
J-Y
JY
Zhou
X-D
XD
D000818:Animals; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics; D003782:Dental Pulp / Q000378:metabolism / Q000473:pathology*; D015536:Down-Regulation; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D009050:Fluorosis, Dental / Q000235:genetics*; D020869:Gene Expression Profiling; D007180:Incisor / Q000473:pathology*; D058574:Integrin-Binding Sialoprotein; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics; D012969:Sodium Fluoride / Q000009:adverse effects
D015820:Cadherins; C081635:Dmp1 protein, rat; D016326:Extracellular Matrix Proteins; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D010750:Phosphoproteins; D012795:Sialoglycoproteins; C086960:osteoblast cadherin; D012969:Sodium Fluoride