[Dimethylsulfoxide-induced expression of thymic stromal lymphopoietin in human bronchial epithelial cells].
OBJECTIVE
To investigate the effect of dimethylsulfoxide on the expression of thymic stromal lymphopoietin (TSLP) in human bronchial epithelial cell (HBE).


METHODS
16HBE cells were incubated in the presence of dimethylsulfoxide at different concentrations, and the cell proliferation changes were observed. The expressions of TSLP mRNA and protein in the cells were detected by real-time quantitative PCR and ELISA, respectively.


RESULTS
Dimethylsulfoxide induced significantly increased TSLP mRNA expression in HBE cells (P<0.01) in a concentration-dependent manner. The level of TSLP protein in the supernatant was also increased after dimethylsulfoxide treatment, but high concentration of dimethylsulfoxide resulted in e inhibited cell proliferation.


CONCLUSIONS
Dimethylsulfoxide may affect the immunomodulatory function of HBE cells.
Zhang
Dan
D
Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. winterpower@163.com
Cai
Shao-xi
SX
Zhao
Hai-jin
HJ
Liu
Lai-yu
LY
D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000094632:Thymic Stromal Lymphopoietin
D016207:Cytokines; D012333:RNA, Messenger; D004121:Dimethyl Sulfoxide; D000094632:Thymic Stromal Lymphopoietin