title: TNF-alpha -308G>A and IL-6 -174G>C polymorphisms in Tunisian patients with coronary artery disease.
abstract: OBJECTIVE
  Our aim was to evaluate the contribution of tumor necrosis factor (TNF)-alpha -308G>A and interleukin (IL)-6 -174G>C gene promoter variants to the presence of coronary artery disease (CAD) in Tunisians.
  
  
  METHODS
  Study subjects comprised 418 angiographically proven CAD patients and 406 age-, gender-, and ethnic origin-matched controls. Genotyping was performed using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis.
  
  
  RESULTS
  There were no significant differences in the allelic distribution of TNF-alpha -308A (19.6% vs. 19.0%, P=0.73), and IL-6 -174C (15.6% vs. 14.3%, P=0.47) promoter polymorphisms between CAD patients and control subjects, respectively. In addition, single locus analysis revealed no differences in genotype frequencies between the two study groups, and the combined distribution of both genotypes did not differ significantly between controls and CAD patients (P>0.05).
  
  
  CONCLUSIONS
  There is no allelic or genotypic association of TNF-alpha -308G>A and IL-6 -174G>C promoter polymorphisms with CAD in Tunisians, thereby confirming an ethnic-selective contribution of both gene variants to CAD presence.
authors.affiliation: Research Unit of Biology and Genetics of Cancer, Haematological and Autoimmune Diseases, Faculty of Pharmacy of Monastir, Monastir 5000, Tunisia. ghazouani2005@yahoo.fr
authors.forename: Lakhdar
authors.initials: L
authors.lastname: Ghazouani
authors.forename: Sonia
authors.initials: S
authors.lastname: Ben Hadj Khalifa
authors.forename: Nesrine
authors.initials: N
authors.lastname: Abboud
authors.forename: Khaldoun
authors.initials: K
authors.lastname: Ben Hamda
authors.forename: Ali
authors.initials: A
authors.lastname: Ben Khalfallah
authors.forename: Nsiri
authors.initials: N
authors.lastname: Brahim
authors.forename: Wassim Youssef
authors.initials: WY
authors.lastname: Almawi
authors.forename: Touhami
authors.initials: T
authors.lastname: Mahjoub
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014416:Tunisia
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha