Relation between serotype distribution and antibiotic resistance profiles of Listeria monocytogenes isolated from ground turkey.
The objectives of this study were to determine the serotype distribution of Listeria monocytogenes isolated from ground turkey using a multiplex PCR assay and to determine antimicrobial resistance profiles of the isolates using the disc diffusion method. Of 78 isolates, 35 (44.9%), 29 (37.2%), 7 (9.0%), and 7 (9.0%) were identified as serotypes 1/2a (or 3a), 4b (or 4d or 4e), 1/2b (or 3b), and 1/2c (or 3c), respectively. Overall, 63 isolates (80.8%) were resistant to penicillin G, and 53 (67.9%) were resistant to ampicillin. All 1/2c (or 3c) serotype isolates were resistant to penicillin G and ampicillin, and all 1/2b (or 3b) serotype isolates were resistant to penicillin G. In addition, 91.4% (32 of 35) of 1/2a (or 3a), 57.1% (4 of 7) of 1/2b (or 3b), and 37.9% (11 of 29) of 4b (or 4d or 4e) serotype isolates were resistant to ampicillin, and 85.7% (30 of 35) of 1/2a (or 3a) and 65.5% (19 of 29) of 4b (or 4d or 4e) serotype isolates were resistant to penicillin G. In conclusion, most of the L. monocytogenes isolates identified were serotype 1/2a (or 3a) and 4b (or 4d or 4e). Serotype 1/2c (or 3c) isolates were highly resistant to antibiotics compared with isolates of serotypes 1/2a (or 3a), 1/2b (or 3b), and 4b (or 4d or 4e). Increasing resistance of L. monocytogenes to ampicillin and penicillin is an especially serious concern for public health because of the common use of these antibiotics in treatment of human listeriosis cases.
Ayaz
Naim Deniz
ND
Department of Food Hygiene and Technology, School of Veterinary Medicine, Ankara University, 06110 Diskapi, Ankara, Turkey.
Erol
Irfan
I
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011202:Poultry Products / Q000382:microbiology*; D012703:Serotyping; D014422:Turkeys
D000900:Anti-Bacterial Agents