title: Rapid diagnosis of tuberculosis and multidrug resistance with the microscopic observation drug susceptibility assay in Ecuador.
abstract: A collaborative project was established between the Alli Causai Foundation in Ambato, Ecuador, and the University of Genoa, Italy, to introduce the microscopic observation drug susceptibility (MODS) assay for the rapid identification of Mycobacterium tuberculosis in Ecuador. A total of 507 samples were evaluated during a 10-month period, and DNA was extracted from each isolate and sent to Genoa for confirmatory molecular analysis. M. tuberculosis was identified in 45 samples by MODS, and drug resistance was observed in approximately 21% of the isolates, with four multidrug-resistant strains detected in two patients.
authors.affiliation: Division of Microbiology CA Romanzi, Dipartimento Interdisciplinare di Scienze Specialistiche Chirurgiche, di Microbiologia e Dei Trapianti d'Organo, University of Genoa, Genoa, Italy. claudio.giacomazzi@unige.it
authors.forename: C G
authors.initials: CG
authors.lastname: Giacomazzi
authors.forename: C G
authors.initials: CG
authors.lastname: Cespedes-Alvarado
authors.forename: E A
authors.initials: EA
authors.lastname: Losada-Cabruja
authors.forename: J L
authors.initials: JL
authors.lastname: McDermott
authors.forename: C A
authors.initials: CA
authors.lastname: Rojas-Andrade
authors.forename: O E
authors.initials: OE
authors.lastname: Varnier
mesh_terms: D000995:Antitubercular Agents / Q000627:therapeutic use*; D015169:Colony Count, Microbial / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004484:Ecuador / Q000453:epidemiology; D006801:Humans; D015994:Incidence; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012189:Retrospective Studies; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial