Antibacterial nitric oxide-releasing polyester for the coating of blood-contacting artificial materials.
The emergence of multidrug-resistant bacteria associated with blood-contacting artificial materials is a growing health problem, which demands new approaches in the field of biomaterials research. In this study, a poly(sulfhydrylated polyester) (PSPE) was synthesized by the polyesterification reaction of mercaptosuccinic acid with 3-mercapto-1,2-propanediol and blended with poly(methyl methacrylate) (PMMA) from solution, leading to solid PSPE/PMMA films, with three different PSPE : PMMMA mass ratios. These films were subsequently S-nitrosated through the immersion in acidified nitrite solution, yielding poly(nitrosated)polyester/PMMA (PNPE/PMMA) films. A polyurethane intravascular catheter coated with PNPE/PMMA was shown to release nitric oxide (NO) in phosphate buffered saline solution (pH 7.4) at 37 degrees C at rates of 4.6 nmol/cm(2)/h in the first 6 h and 0.8 nmol/cm(2)/h in the next 12 h. When used to coat the bottom of culture plates, NO released from these films exerted a potent dose- and time-dependent antimicrobial activity against Staphylococcus aureus and a multidrug-resistant Pseudomonas aeruginosa strains. This antibacterial effect of PSPE/PMMA films opens a new perspective for the coating of blood-contacting artificial materials, for avoiding their colonization with highly resistant bacteria.
Seabra
Amedea B
AB
Institute of Chemistry, University of Campinas, UNICAMP, Campinas SP, Brazil.
Martins
Dorival
D
Simões
Maíra M S G
MM
da Silva
Regiane
R
Brocchi
Marcelo
M
de Oliveira
Marcelo G
MG
D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D002408:Catheters, Indwelling; D009569:Nitric Oxide / Q000008:administration & dosage* / Q000494:pharmacology*; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D019904:Polymethyl Methacrylate / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D011091:Polyesters; D013438:Sulfhydryl Compounds; D009569:Nitric Oxide; D019904:Polymethyl Methacrylate