The selective role of myosin VI in lymphoid leukemia cell migration.
Several myosin isotypes are discussed to be involved in the migration of various cells ranging from tumor cells to leukocytes. We investigated the involvement of myosins II and VI in the lymphoid leukemia cells lines Jurkat, NB4, Dohh-2, and Molt-4 by a three-dimensional, collagen-based migration assay. Down-regulation of myosin VI by siRNA significantly reduced the migratory activity of all cells, whereas the pharmacological inhibition of non-muscle myosin II using blebbistatin had only marginal influence. Therefore, in contrast to differentiated leukocytes and cells from solid tumors, myosin VI plays a crucial role in the migration of leukemic cells.
Jbireal
Jbireal M Ali
JM
Institute of Immunology, Witten/Herdecke University, Witten, Germany.
Strell
Carina
C
Niggemann
Bernd
B
Zänker
Kurt
K
Entschladen
Frank
F
D002465:Cell Movement; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism*; D024462:Myosin Type II / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics
D006576:Heterocyclic Compounds, 4 or More Rings; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; C089785:myosin VI; C472645:blebbistatin; D024462:Myosin Type II; D018995:Myosin Heavy Chains