Comparison of methods of detecting bacterial biofilm.
Fresh bacterial biofilm produced by Staphylococcus epidermidis was sensitively detected by using a chemiluminescent probe. Its lower detection limit was around 10(-5) - 10(-4) dilution (equivalent to 10(-5) - 10(-4) cm2 biofilm) - 10(2) - 10(3) times as sensitive as ordinary colorimetric methods. The linearity and dynamic range were slightly better than those of fluorometry.
Tajima
Yutaka
Y
Legal Medicine and Molecular Genetics, Postgraduate School of Medicine, Gunma University, Maebashi 371-8511, Japan. y-tajima@koaikai.or.jp
Yamaguchi
Tatsuo
T
Takagi
Rie
R
Nakajima
Tamiko
T
Kominato
Yoshihiko
Y
D018441:Biofilms / Q000254:growth & development*; D003124:Colorimetry; D003470:Culture Media; D005109:Extracellular Matrix / Q000502:physiology; D005453:Fluorescence; D049449:Luminescence; D012680:Sensitivity and Specificity; D013212:Staphylococcus epidermidis / Q000502:physiology*; D014909:Wheat Germ Agglutinins / Q000032:analysis
D003470:Culture Media; D014909:Wheat Germ Agglutinins