Prevotella fusca sp. nov. and Prevotella scopos sp. nov., isolated from the human oral cavity.
Two strains of anaerobic, Gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to belong to two separate taxa. Phylogenetic analysis of full-length 16S rRNA gene sequences showed that the strains were both related to, but distinct from, the type strain of Prevotella melaninogenica. Two novel species, Prevotella fusca sp. nov. and Prevotella scopos sp. nov., are proposed to accommodate these strains. Both strains were saccharolytic and produced acetic and succinic acids, with lesser amounts of lactic and isovaleric acids, as end products of fermentation, and both were sensitive to 20 % bile. The principal cellular long-chain fatty acids of both strains were ai-C(15 : 0), 3-OH i-C(17 : 0), 3-OH C(16 : 0), i-C(15 : 0) and C(16 : 0). The DNA G+C contents of the type strains of Prevotella fusca (W1435(T)  = DSM 22504(T)  = CCUG 57946(T)) and Prevotella scopos (W2052(T)  = DSM 22613(T ) = CCUG 57945(T)) were 43 and 41 mol%, respectively. The two species could be differentiated by gelatin hydrolysis, cellobiose and ribose fermentation, and production of β-glucosidase.
Downes
Julia
J
King's College London Dental Institute at Guy's, King's College and St Thomas' Hospitals, Infection Research Group, Guy's Campus, London SE1 9RT, UK.
Wade
William G
WG
King's College London Dental Institute at Guy's, King's College and St Thomas' Hospitals, Infection Research Group, Guy's Campus, London SE1 9RT, UK.
D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000378:metabolism; D001482:Base Composition; D001646:Bile / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D045782:Hemiterpenes; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology*; D010421:Pentanoic Acids / Q000378:metabolism; D010802:Phylogeny; D018720:Prevotella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019802:Succinic Acid / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D045782:Hemiterpenes; D010421:Pentanoic Acids; D012336:RNA, Ribosomal, 16S; C008216:isovaleric acid; D019344:Lactic Acid; D013395:Sucrose; D019802:Succinic Acid