Transmembrane 4 L six family member 5 (TM4SF5) enhances migration and invasion of hepatocytes for effective metastasis.
Overexpression of transmembrane 4 L six family member 5 (TM4SF5), a four-transmembrane L6 family member, causes aberrant cell proliferation and angiogenesis, but the roles of TM4SF5 in migration, invasion, and tumor metastasis remain unknown. Using in vitro hepatocarcinoma cells that ectopically or endogenously express TM4SF5 and in vivo mouse systems, roles of TM4SF5 in metastatic potentials were examined. We found that TM4SF5 expression facilitated migration, invadopodia formation, MMP activation, invasion, and eventually lung metastasis in nude mice, but suppression of TM4SF5 with its shRNA blocked the effects. Altogether, TM4SF5-mediated migration and invasion suggest that TM4SF5 may be therapeutically targeted to deal with TM4SF5-mediated hepatocellular cancers.
Lee
Sin-Ae
SA
Cancer Research Institute, Seoul National University, Seoul 110-799, Korea.
Kim
Tae You
TY
Kwak
Tae Kyoung
TK
Kim
Hyeonjung
H
Kim
Semi
S
Lee
Hyo Jeong
HJ
Kim
Sung-Hoon
SH
Park
Ki Hun
KH
Kim
Hyun Jeong
HJ
Cho
Moonjae
M
Lee
Jung Weon
JW
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D022781:Hepatocytes / Q000166:cytology / Q000473:pathology / Q000502:physiology; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis
D008565:Membrane Proteins; C111029:TM4SF5 protein, human