title: Phylogenetic analysis and prokaryotic expression of NMAAP1 derived from BCG-activated murine macrophages.
abstract: BCG-activated macrophages exerted anti-tumor activities. Cell surface molecules play an important role in mediating endocytosis by macrophages. In the previous study, we identified a group of 454 membrane proteins specifically expressed on BCG-activated mouse macrophages, including a protein named NMAAP1 (novel macrophage activated associated protein). In this study, we aligned the full-length nucleotide sequences of NMAAP1 and its homologous sequences to construct its phylogenetic tree, and cloned the NMAAP1 cDNA from BCG-activated macrophages to generated NMAAP1 fusion protein in Escherichia coli. Purified the fusion protein were applied for generation of polyclonal antibodies. Western-blotting detection showed that the polyclonal antibodies have high specificities to recognize target protein.
authors.affiliation: Department of Immunology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China. zxf184@163.com
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Zhao
authors.forename: Linbing
authors.initials: L
authors.lastname: Zhang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Yan
authors.forename: Xiuping
authors.initials: X
authors.lastname: Feng
authors.forename: Dun
authors.initials: D
authors.lastname: Chu
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Bairong
authors.initials: B
authors.lastname: Du
authors.forename: Xun
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors / Q000235:genetics; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000276:immunology*; D010802:Phylogeny; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
chemical_list: D008565:Membrane Proteins; C561300:NMAAP1 protein, mouse; D011994:Recombinant Proteins