Synthesis and application of organomercury haptens for enzyme-linked immunoassay of inorganic and organic mercury.
Two organomercury haptens were synthesized via the classical oxymercuration reaction. An intramolecular oxymercuration reaction was the strategy employed to prepare a structurally simple, but chemically robust, organomercury hapten that was conjugated to chicken immunoglobulin G (IgG). The resulting immunogen afforded mouse anti-mercury antibodies that were evaluated in an enzyme-linked immunosorbent assay (ELISA). Antibodies demonstrating high titers were obtained, and various immunoassay parameters were investigated. The sensitivity and selectivity of the resulting antibodies were evaluated by exploring different cross-coupling chemistries and solid-phase synthetic variations. A second hapten was prepared with the intermolecular oxymercuration reaction, and the resulting compound, once coupled to carrier protein, afforded a solid-phase conjugate that revealed the versatility of the mouse anti-mercury antibody. The anti-mercury antibody developed in this study was capable of detecting both mercury(II) salts and organomercury compounds.
Prudenté
Caryn K
CK
Department of Chemistry, University of Southern Maine, Portland, ME 04104, USA. prudente@maine.edu
Sirios
Rebekah S
RS
Cote
Shaun
S
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006241:Haptens / Q000276:immunology*; D007074:Immunoglobulin G / Q000737:chemistry; D051379:Mice; D009941:Organomercury Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology*
D000906:Antibodies; D006241:Haptens; D007074:Immunoglobulin G; D009941:Organomercury Compounds