Degradation of microcystin-LR and RR by a Stenotrophomonas sp. strain EMS isolated from Lake Taihu, China.
A bacterial strain EMS with the capability of degrading microcystins (MCs) was isolated from Lake Taihu, China. The bacterium was tentatively identified as a Stenotrophomonas sp. The bacterium could completely consume MC-LR and MC-RR within 24 hours at a concentration of 0.7 microg/mL and 1.7 microg/mL, respectively. The degradation of MC-LR and MC-RR by EMS occurred preferentially in an alkaline environment. In addition, mlrA gene involved in the degradation of MC-LR and MC-RR was detected in EMS. Due to the limited literature this gene has rare homologues. Sequencing analysis of the translated protein from mlrA suggested that MlrA might be a transmembrane protein, which suggests a possible new protease family having unique function.
Chen
Jian
J
Institute of Food Safety and Quality, Jiangsu Academy of Agricultural Sciences, Nanjing, China. chenjian@jaas.ac.cn
Hu
Liang Bin
LB
Zhou
Wei
W
Yan
Shao Hua
SH
Yang
Jing Dong
JD
Xue
Yan Feng
YF
Shi
Zhi Qi
ZQ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D002681:China; D007536:Isomerism; D060106:Lakes / Q000382:microbiology; D052998:Microcystins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D020588:Stenotrophomonas / Q000302:isolation & purification / Q000378:metabolism*
D001426:Bacterial Proteins; D052998:Microcystins; C078588:microcystin