Increased viability of endometrial cells by in vitro treatment with di-(2-ethylhexyl) phthalate.
Based on the findings of several reports that have shown an increased plasma level of di-(2-ethylhexyl) phthalate (DEHP) in women with endometriosis, the present study was designed to evaluate whether in vitro treatment with DEHP can increase viability of endometrial cells. Utilizing 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide assay, fluorescent activated cell sorter analysis, and microscopic evaluation after Hoechst staining, we revealed that in vitro treatment with DEHP leads to increased viability of Ishikawa cells as well as endometrial stromal cells in serum-free condition and following exposure to hydrogen peroxide, which suggests that exposure to phthalate might play a role in the establishment of endometriosis.
Kim
Young-Hoon
YH
Department of Pharmacology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea.
Kim
Sung Hoon
SH
Lee
Hoi Woul
HW
Chae
Hee Dong
HD
Kim
Chung-Hoon
CH
Kang
Byung Moon
BM
D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004717:Endometrium / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006801:Humans; D010968:Plasticizers / Q000494:pharmacology; D017154:Stromal Cells / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects
D010968:Plasticizers; D004051:Diethylhexyl Phthalate