title: Extraction of biomolecules using phosphonium-based ionic liquids + K(3)PO(4) aqueous biphasic systems.
abstract: Aqueous biphasic systems (ABS) provide an alternative and efficient approach for the extraction, recovery and purification of biomolecules through their partitioning between two liquid aqueous phases. In this work, the ability of hydrophilic phosphonium-based ionic liquids (ILs) to form ABS with aqueous K(3)PO(4) solutions was evaluated for the first time. Ternary phase diagrams, and respective tie-lines and tie-lines length, formed by distinct phosphonium-based ILs, water, and K(3)PO(4) at 298 K, were measured and are reported. The studied phosphonium-based ILs have shown to be more effective in promoting ABS compared to the imidazolium-based counterparts with similar anions. Moreover, the extractive capability of such systems was assessed for distinct biomolecules (including amino acids, food colourants and alkaloids). Densities and viscosities of both aqueous phases, at the mass fraction compositions used for the biomolecules extraction, were also determined. The evaluated IL-based ABS have been shown to be prospective extraction media, particularly for hydrophobic biomolecules, with several advantages over conventional polymer-inorganic salt ABS.
authors.affiliation: Departamento de Química, CICECO, Universidade de Aveiro, Portugal.
authors.forename: Cláudia L S
authors.initials: CL
authors.lastname: Louros
authors.forename: Ana Filipa M
authors.initials: AF
authors.lastname: Cláudio
authors.forename: Catarina M S S
authors.initials: CM
authors.lastname: Neves
authors.forename: Mara G
authors.initials: MG
authors.lastname: Freire
authors.forename: Isabel M
authors.initials: IM
authors.lastname: Marrucho
authors.forename: Jérôme
authors.initials: J
authors.lastname: Pauly
authors.forename: João A P
authors.initials: JA
authors.lastname: Coutinho
mesh_terms: D002110:Caffeine / Q000302:isolation & purification; D052578:Ionic Liquids / Q000737:chemistry*; D007700:Kinetics; D059625:Liquid-Liquid Extraction; D010710:Phosphates / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*; D012235:Rhodamines / Q000302:isolation & purification; D013696:Temperature; D014364:Tryptophan / Q000302:isolation & purification; D014783:Viscosity; D014867:Water / Q000737:chemistry*; D019207:beta Carotene / Q000302:isolation & purification
chemical_list: D052578:Ionic Liquids; D010710:Phosphates; D017680:Potassium Compounds; D012235:Rhodamines; D019207:beta Carotene; C026188:rhodamine 6G; D014867:Water; D002110:Caffeine; D014364:Tryptophan; C013216:potassium phosphate