Characterization of ultra-thin temperature-responsive polymer layer and its polymer thickness dependency on cell attachment/detachment properties.
Ultra thin poly(N-isopropylacrylamide) (PIPAAm) modified glass coverslips (PIAPAm-CS) using electron beam irradiation exhibited a clear relationship between the polymer thickness and thermal cell adhesion/detachment behavior. The polymer thickness dependency and the characteristic of ultra thin PIPAAm layer, has been illustrated in terms of the molecular motion of the modified PIPAAm chains. PIPAAm-CSs surfaces with various area-polymer densities and thicknesses were characterized by AFM and protein adsorption assay. The newly obtained results gave a further insight into the illustration. Finally, the future application of intelligent surfaces was discussed for fabricating tissue and organ.
Fukumori
Kazuhiro
K
Department of Applied Chemistry, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo, Japan.
Akiyama
Yoshikatsu
Y
Kumashiro
Yoshikazu
Y
Kobayashi
Jun
J
Yamato
Masayuki
M
Sakai
Kiyotaka
K
Okano
Teruo
T
D000180:Acrylic Resins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D005898:Glass / Q000737:chemistry; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D013499:Surface Properties; D013696:Temperature
D000180:Acrylic Resins; D001672:Biocompatible Materials; C052970:poly-N-isopropylacrylamide