[HBD-1 and hBD-2 are expressed in cervico-vaginal lavage in female genital tract due to microbial infections].
OBJECTIVE
The aim of this study was to evaluate and compare concentration of selected human beta-defensins (hBD-1, hBD-2) in cervico-vaginal lavage (CVL), obtained from women with candidiasis, chlamydiasis and other bacterial infections.


METHODS
beta-defensins were detected quantitatively by RT-PCR (7000 Taqman, Applied Biosystems) in cervico-vaginal lavage collected from 120 (79 women in the study group and 41 controls) non-pregnant women, aged 18-40 (mean age 28.5 +/- 6.29). The study group patients were divided into three subgroups on the basis of clinical and microbiological diagnosis: women with candidiasis (n=13); with chlamydiasis (n=13), and with other bacterial infections (n=12).


RESULTS
The highest count of hBD-1 RNA copies was found in women with bacterial infections and candidiasis (335.84 and 320.10 respectively), and hBD-2--with chlamydiasis. The difference between RNA copies of hBD-1/microg in candidiasis, chlamydiasis and bacterial pathogens was statistically significant; for hBD-2 only in case of chlamydiasis.


CONCLUSIONS
Chlamydia trachomatis infection activates the production of hBD-2. Candida albicans, Chlamydia trachomatis, and bacterial pathogens induced variable increases of hBD-1 concentration.
Wiechuła
Barbara
B
Katedra i Zakład Mikrobiologii Lekarskiej Slaskiego Uniwersytetu Medycznego.
Cholewa
Krzysztof
K
Ekiel
Alicja
A
Romanik
Małgorzata
M
Dolezych
Hanna
H
Martirosian
Gayane
G
D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002181:Candidiasis, Vulvovaginal / Q000175:diagnosis* / Q000378:metabolism; D002582:Cervix Mucus / Q000382:microbiology*; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000378:metabolism; D015169:Colony Count, Microbial; D005260:Female; D005831:Genital Diseases, Female / Q000175:diagnosis* / Q000378:metabolism* / Q000382:microbiology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015231:Sexually Transmitted Diseases, Bacterial / Q000175:diagnosis / Q000378:metabolism; D014621:Vagina / Q000378:metabolism; D014626:Vaginal Smears / Q000379:methods; D055815:Young Adult; D023083:beta-Defensins / Q000378:metabolism*
D015415:Biomarkers; C406407:DEFB1 protein, human; C106641:DEFB4A protein, human; D023083:beta-Defensins