Anti-CD4-targeted gold nanoparticles induce specific contrast enhancement of peripheral lymph nodes in X-ray computed tomography of live mice.
Antibody-conjugated gold nanoparticles have been applied as a biologically targeted contrast agent in live mice for one of the most widely used medical imaging methods, X-ray computed tomography. Such nanoprobes directed toward the CD4 receptor lead to distinctly enhanced X-ray contrast of peripheral lymph nodes. This study demonstrates the general feasibility of biologically specific X-ray imaging in living animals and discusses basic requirements for the use of nanoparticles as a targeted X-ray contrast agent.
Eck
Wolfgang
W
Applied Physical Chemistry, University of Heidelberg, Heidelberg, Germany. w-eck@uni-hd.de
Nicholson
Anthony I
AI
Zentgraf
Hanswalter
H
Semmler
Wolfhard
W
Bartling
Sönke
S
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology*; D003287:Contrast Media / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008198:Lymph Nodes / Q000000981:diagnostic imaging*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry; D014057:Tomography, X-Ray Computed / Q000379:methods*
D000906:Antibodies; D015704:CD4 Antigens; D003287:Contrast Media; D006046:Gold