Evaluation of osteoarthritis induced by treadmill-running exercise using the modified Mankin and the new OARSI assessment system.
To apply the Osteoarthritis Research Society International (OARSI) assessment system to an osteoarthritis model, 44 Wistar rats were randomized into treadmill-running exercise or control group. At 6, 8, and 10 weeks, medial knee joints were histopathologically evaluated, and aggrecan neoepitope and TUNEL staining were performed. Cartilage changes in exercise group were histopathologically and histochemically compatible with early OA. Total modified Mankin system (MMS) scores were significantly higher at all time points (each P ≤ 0.01) in exercise than in control group. However, only tibial OARSI scores of runners were higher at 10 weeks (P < 0.05), although OARSI scores were found to be significantly correlated with MMS scores. Both total MMS (Spearman's coefficient ρ = 0.786) and OARSI scores (ρ = 0.443 for femoral; ρ = 0.604 for tibial) were significantly associated with the exercise duration. In conclusion, the OARSI system may not be sensitive to early OA changes induced by treadmill exercise.
Lee
Yun Jong
YJ
Department of Internal Medicine, Clinical Research Institute, Seoul National University College of Medicine, 28 Yongun-dong, Chongno-gu, Seoul 110-744, Korea.
Park
Ji Ah
JA
Yang
Sei Hoon
SH
Kim
Kwang Yong
KY
Kim
Byoung Kwon
BK
Lee
Eun Young
EY
Lee
Eun Bong
EB
Seo
Jeong-Wook
JW
Echtermeyer
Frank
F
Pap
Thomas
T
Song
Yeong Wook
YW
D053674:Aggrecans / Q000032:analysis; D000818:Animals; D002356:Cartilage / Q000473:pathology; D005080:Exercise Test / Q000009:adverse effects*; D020287:In Situ Nick-End Labeling; D008297:Male; D020370:Osteoarthritis, Knee / Q000209:etiology* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index; D013977:Tibia / Q000473:pathology
D053674:Aggrecans