title: Development of a bioorthogonal and highly efficient conjugation method for quantum dots using tetrazine-norbornene cycloaddition.
abstract: We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.
authors.forename: Hee-Sun
authors.initials: HS
authors.lastname: Han
authors.forename: Neal K
authors.initials: NK
authors.lastname: Devaraj
authors.forename: Jungmin
authors.initials: J
authors.lastname: Lee
authors.forename: Scott A
authors.initials: SA
authors.lastname: Hilderbrand
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
authors.forename: Moungi G
authors.initials: MG
authors.lastname: Bawendi
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D066246:ErbB Receptors / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D006801:Humans; D057054:Molecular Imaging; D009636:Norbornanes / Q000737:chemistry*; D045663:Quantum Dots
chemical_list: D005456:Fluorescent Dyes; D006573:Heterocyclic Compounds, 1-Ring; D009636:Norbornanes; D066246:ErbB Receptors