Zebrafish sp7:EGFP: a transgenic for studying otic vesicle formation, skeletogenesis, and bone regeneration.
We report the expression pattern and construction of a transgenic zebrafish line for a transcription factor involved in otic vesicle formation and skeletogenesis. The zinc finger transcription factor sp7 (formerly called osterix) is reported as a marker of osteoblasts. Using bacterial artificial chromosome (BAC)-mediated transgenesis, we generated a zebrafish transgenic line for studying skeletal development, Tg(sp7:EGFP)b1212. Using a zebrafish BAC, EGFP was introduced downstream of the regulatory regions of sp7 and injected into one cell-stage embryos. In this transgenic line, GFP expression reproduces endogenous sp7 gene expression in the otic placode and vesicle, and in forming skeletal structures. GFP-positive cells were also detected in adult fish, and were found associated with regenerating fin rays post-amputation. This line provides an essential tool for the further study of zebrafish otic vesicle formation and the development and regeneration of the skeleton.
DeLaurier
April
A
Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA. april@uoneuro.uoregon.edu
Eames
B Frank
BF
Blanco-Sánchez
Bernardo
B
Peng
Gang
G
He
Xinjun
X
Swartz
Mary E
ME
Ullmann
Bonnie
B
Westerfield
Monte
M
Kimmel
Charles B
CB
D058500:Animal Fins / Q000502:physiology / Q000601:surgery; D000818:Animals; D030801:Animals, Genetically Modified; D001861:Bone Regeneration / Q000235:genetics*; D019902:Chondrocytes / Q000378:metabolism; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D004423:Ear / Q000196:embryology / Q000254:growth & development; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D017403:In Situ Hybridization; D007814:Larva / Q000235:genetics / Q000378:metabolism; D038081:Organogenesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors; D029961:Zebrafish Proteins; C541742:sp7 protein, zebrafish; D049452:Green Fluorescent Proteins