Identifying drug metallation sites on peptides using electron transfer dissociation (ETD), collision induced dissociation (CID) and ion mobility-mass spectrometry (IM-MS).
Electron transfer dissociation (ETD) and collision induced dissociation (CID) have been used to locate the precise binding sites for platinum and ruthenium anticancer complexes on the peptide Substance P. We show that ETD combined with ion mobility-mass spectrometry significantly reduces mass spectral complexity and improves the S/N of the product-ions formed.
Williams
Jonathan P
JP
Department of Chemistry, University of Warwick, Coventry, UK CV4 7AL.
Brown
Jeffery M
JM
Campuzano
Iain
I
Sadler
Peter J
PJ
D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites; D017671:Platinum Compounds / Q000737:chemistry / Q000494:pharmacology*; D017975:Ruthenium Compounds / Q000737:chemistry / Q000494:pharmacology*; D013373:Substance P / Q000737:chemistry / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000970:Antineoplastic Agents; D017671:Platinum Compounds; D017975:Ruthenium Compounds; D013373:Substance P