Association of tailed acetylcholinesterase to lipidic membranes in mammalian skeletal muscle.
Tailed acetylcholinesterase (AChE) was studied in three subcellular membrane fractions of mouse skeletal muscle: a fraction enriched in isolated motor endplates (C), an extrasynaptic membrane fraction (A) and a microsomal fraction (S). In the (C) fraction, tailed asymmetric 16S AChE required high salt conditions to be extracted, while in (A) and (S) microsomal membranes, a collagenase sensitive 16S form, was extracted by detergent alone. This apparent "hydrophobic" property suggests that there is a pool of 16S AChE which is probably bound to lipidic membranes. The detergent extractable (DE) 16S AChE was not concentrated in motor endplate-rich regions and differential inhibition of external and internal AChE demonstrated that it could have both intra- and extracellular locations in the adult differentiated muscle fibres.
Garcia
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L
Institut National de la Santé et de la Recherche Médicale U.153, 17 rue du Fer-à-Moulin, 75005 Paris France.
Verdière-Saruqué
M
M
Dreyfus
P A
PA
Nicolet
M
M
Rieger
F
F