A role of piperine on monosodium urate crystal-induced inflammation--an experimental model of gouty arthritis.
In the present study, the anti-inflammatory effect of piperine was investigated on monosodium urate crystal-induced inflammation in mice, an experimental model for gouty arthritis, and compared it with that of the nonsteroidal anti-inflammatory drug, indomethacin. The levels of lysosomal enzymes, lipid peroxidation, tumor necrosis factor-α, and paw volume were increased significantly, and the activities of antioxidant status were in turn decreased in monosodium urate crystal-induced mice, whereas these changes were reverted to near normal levels upon piperine (30 mg/kg b.wt, i.p.) treatment. In vitro, piperine (50/100 ug/ml) suppressed the level of β-glucuronidase and lactate dehydrogenase in monosodium urate crystal-incubated polymorphonuclear leucocytes in concentration-dependent manner when compared to control cells. Thus, the present study clearly indicated that piperine inhibit the monosodium urate crystal-induced inflammation and can be regarded as therapeutic drug for the treatment of acute gouty arthritis.
Sabina
Evan Prince
EP
School of Bio Sciences and Technology, VIT University, Vellore, 632 014, Tamil Nadu, India.
Nagar
Shruthi
S
Rasool
Mahaboobkhan
M
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D015210:Arthritis, Gouty / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D052117:Benzodioxoles / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology; D010880:Piperidines / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014527:Uric Acid / Q000633:toxicity
D000470:Alkaloids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D052117:Benzodioxoles; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D014409:Tumor Necrosis Factor-alpha; D014527:Uric Acid; C008922:piperine; D007213:Indomethacin