Antioxidative status and lipid peroxidation in kidney tissue of rats fed with vitamin B(6)-deficient diet.
BACKGROUND
The aim of this study was to evaluate lipid peroxidation (LP) and free radical scavenging enzyme activities in kidney tissue of vitamin B(6)-deficient rats.


METHODS
The rats were divided into control and vitamin B(6)-deficient groups. After 4 weeks of feeding, animals in all groups were anesthetized by thiopental sodium (50 mg/kg). Thoraces were opened, 2 mL blood samples were taken from aortas, then the rats were killed by cervical dislocation, and kidney tissues were removed. Biochemical measurements in kidney tissue were carried out using a spectrophotometer.


RESULTS
Total superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), superoxide dismutase (SOD) activities, and antioxidant potential (AOP) values in the vitamin B(6)-deficient group were significantly lower than those of the control group, whereas glutathione peroxidase (GSH-Px), glutathione reductase (GRD), glutathione-S-transferase (GST) activities, and malondialdehyde (MDA) level were significantly higher than those of the control group (p < 0.05).


CONCLUSIONS
The results show that vitamin B(6) deficiency causes an attenuation in antioxidant defense system and an increase in oxidative stress in kidney tissue of rats.
Keles
Mustafa
M
Department of Internal Medicine, Division of Nephrology, School of Medicine, Ataturk University, Erzurum, Turkey.
Al
Behcet
B
Gumustekin
Kenan
K
Demircan
Berna
B
Ozbey
Isa
I
Akyuz
Mehmet
M
Yilmaz
Adnan
A
Demir
Elif
E
Uyanik
Abdullah
A
Ziypak
Tevfik
T
Taysi
Seyithan
S
D000818:Animals; D016166:Free Radical Scavengers / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D026681:Vitamin B 6 Deficiency / Q000378:metabolism*
D016166:Free Radical Scavengers