title: The fatigue resistance of rabbit tibiae varies with age from youth to middle age.
abstract: Young adults are at risk of stress fractures. Risk is higher in younger and female individuals. Stress fractures occur due to repeated loading of the bone (fatigue). We modeled this with rabbit tibiae. Age increased fatigue resistance which correlated with bone mineral density. A sex difference was not detected.
  
  
  BACKGROUND
  Younger adults who engage in intense physical activity with a sudden increase in intensity level (military recruits/college athletes) are at risk of bone stress fractures. Risk is greater in females and diminishes with aging. Stress fractures may be the result of fatigue damage, which is not repaired rapidly enough to avoid fracture. It was hypothesized that the fatigue resistance of whole rabbit tibiae would be less in female specimens but greater as animal age increased.
  
  
  METHODS
  Rabbit tibiae were harvested from three age groups (4, 7, and ≥ 12 months (females only)). The tibiae were scanned with dual energy X-ray absorptiometry to determine bone mineral density (BMD), computed tomography to quantify geometry, and then fatigue tested in three-point bending.
  
  
  RESULTS
  In the ≥ 12-month group, BMD was approximately 20% higher, while the fatigue resistance was found to be approximately ten times higher than the other age groups. Sex was not a factor in the 4- and 7-month groups. Multiple linear regression revealed that fatigue life was negatively correlated with applied stress range and positively correlated with BMD (adjusted r (2) = 0.69).
  
  
  CONCLUSIONS
  A difference in fatigue behavior due to sex was not detected, but there was a large increase in fatigue resistance with age. This correlated with increased BMD and parallels a reduced risk of stress fracture due to age in military recruits. Skeletal "maturation" may play an important role in determining stress fracture risk. Increased risk in females may be due to mechanisms other than those that determine material behavior.
authors.affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
authors.forename: T L
authors.initials: TL
authors.lastname: Willett
authors.forename: C
authors.initials: C
authors.lastname: Wynnyckyj
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: M D
authors.initials: MD
authors.lastname: Grynpas
mesh_terms: D015502:Absorptiometry, Photon; D000375:Aging / Q000502:physiology*; D000818:Animals; D015519:Bone Density / Q000502:physiology; D005260:Female; D015775:Fractures, Stress / Q000503:physiopathology*; D008297:Male; D011817:Rabbits; D012307:Risk Factors; D012737:Sex Factors; D013314:Stress, Mechanical; D013977:Tibia / Q000503:physiopathology*; D013978:Tibial Fractures / Q000503:physiopathology