title: The Glc2Man2-fragment of the N-glycan precursor--a novel ligand for the glycan-binding protein malectin?
abstract: The Glcalpha(1-->3)Glcalpha(1-->3)Manalpha(1-->2)Man tetrasaccharide (Glc(2)Man(2)-fragment), a substructure of the natural N-glycan precursor, was synthesized. The interaction of this fragment with the protein malectin, a carbohydrate binding protein localized in the endoplasmatic reticulum, was investigated by (1)H(15)N HSQC experiments and isothermal calorimetry. The chemical shift perturbations of nuclei in the protein's backbone caused by the binding of the Glc(2)Man(2)-fragment to malectin suggest a binding mode like the known ligand nigerose.
authors.affiliation: Karlsruher Institut für Technologie-KIT, Institut für Organische Chemie, Fritz-Haber-Weg 6, 76131, Karlsruhe, Germany.
authors.forename: Lisa N
authors.initials: LN
authors.lastname: Müller
authors.forename: Claudia
authors.initials: C
authors.lastname: Muhle-Goll
authors.forename: Moritz B
authors.initials: MB
authors.lastname: Biskup
mesh_terms: D002240:Carbohydrate Sequence; D000939:Epitopes / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008024:Ligands; D008358:Mannose / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry*; D011485:Protein Binding
chemical_list: D000939:Epitopes; D037102:Lectins; D008024:Ligands; D009844:Oligosaccharides; D011134:Polysaccharides; D008358:Mannose