[Experiment on inducing human periodontal ligament stem cells into adipose cells].
OBJECTIVE
To explore the capability of human periodontal ligament stem cells (PDLSCs) differentiating into adipose cells in vitro and to determine their changes in cell morphology, structure and function during differentiation.


METHODS
PDLSCs isolated by magnetic-activated cell selection were treated continuously with adipogenic medium for 21 d. Then the cell morphology, ultrastructure, adipose specific markers of low density lipoprotein (LPL) and peroxisome proliferator activated receptor-gamma (PPAR-gamma) were analyzed by inverted contrast microscope, trans mission electron microscope (TEM), flow cytometry, immunofluorescence, RT-PCR and Western blot, respectively. These adipose-like cells were also identified by oil red O staining to determine the formation of lipid droplet, and the non-induced cells were used as control.


RESULTS
After continuous induction, the treated cells differentiated into adipose-like cells with round shape, and large amount of lipid drop in cytoplasm. 96.54% of the PDLSCs were found to differentiate into adipose cells as showed by flow cytometry, the specific markers of LPL mRNA and PPAR-gamma mRNA, and oil red O staining, respectively. Further, PPAR-gamma protein was detected in the induced cells in a time-dependent manner.


CONCLUSIONS
Human PDLSCs have the potential of differentiating into adipose cells under appropriate condition, and the differentiated cells exhibited characteristics of adipose cells both from cell morphology and from their functions.
He
Hui-xia
HX
Institute of Dental Research, General Hospital of Chinese PLA, Beijing 100853, China.
Liu
Hong-chen
HC
Wang
Dong-sheng
DS
Cao
Jun-kai
JK
Zhang
Hai-zhong
HZ
E
Ling-ling
LL
D017667:Adipocytes; D002454:Cell Differentiation; D006801:Humans; D047495:PPAR gamma; D010513:Periodontal Ligament; D013234:Stem Cells
D047495:PPAR gamma