title: First survey of metallo-beta-lactamases in clinical isolates of Pseudomonas aeruginosa in a German university hospital.
abstract: A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-beta-lactamase (MBL) production. Molecular analysis detected a blaVIM-1 gene in the chromosome of one isolate and a blaVIM-2 gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory EDTA combination disk test represents a valid alternative to the molecular investigation of MBL genes, making MBL detection possible in routine diagnostic laboratories.
authors.affiliation: Institute of Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Str. 2/E1, 97080 Würzburg, Germany. gvalenza@hygiene.uni-wuerzburg.de
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Valenza
authors.forename: Biju
authors.initials: B
authors.lastname: Joseph
authors.forename: Johannes
authors.initials: J
authors.lastname: Elias
authors.forename: Heike
authors.initials: H
authors.lastname: Claus
authors.forename: Anett
authors.initials: A
authors.lastname: Oesterlein
authors.forename: Kathrin
authors.initials: K
authors.lastname: Engelhardt
authors.forename: Doris
authors.initials: D
authors.lastname: Turnwald
authors.forename: Matthias
authors.initials: M
authors.lastname: Frosch
authors.forename: Marianne
authors.initials: M
authors.lastname: Abele-Horn
authors.forename: Christoph
authors.initials: C
authors.lastname: Schoen
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D005858:Germany / Q000453:epidemiology; D006785:Hospitals, University / Q000706:statistics & numerical data*; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; D002442:Ceftazidime; D001618:beta-Lactamases