title: Molecular characterization of early adenocarcinoma of the uterine cervix by oligonucleotide microarray.
abstract: OBJECTIVE
  In an attempt to understand the molecular characterization for the early adenocarcinoma of the uterine cervix, an analysis of gene expression profiles obtained from early adenocarcinoma of the uterine cervix was performed to find those genes most aberrantly expressed.
  
  
  METHODS
  Total RNA was prepared from 32 samples of early adenocarcinoma of the uterine cervix and 32 paired normal cervix tissues, and hybridized to cancer-associated oligonucleotide microarrays with probe sets complementary to about 1,426 transcripts.
  
  
  RESULTS
  Supervised analysis of gene expression data identified 13 genes that exhibited >2-fold upregulation and 27 genes >2-fold downregulation, respectively, in early adenocarcinoma of the uterine cervix compared to normal cervix. Unsupervised hierarchical clustering of the expression data readily distinguished early adenocarcinoma of the uterine cervix from normal cervix. Two genes (karyopherin alpha 2 and proliferating cell nuclear antigen) were selected randomly for real-time reverse transcription polymerase chain reaction analysis. Both genes were expressed significantly higher in early adenocarcinoma of the uterine cervix than in normal cervix, with p = 0.0003 and <0.0001, respectively. These results were compatible with the microarray data.
  
  
  CONCLUSIONS
  This study has revealed several genes that may be highly attractive candidate molecular markers/targets for early adenocarcinoma of the uterine cervix.
authors.affiliation: Department of Radiology, First Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China.
authors.forename: Ling-Min
authors.initials: LM
authors.lastname: Liao
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.forename: Long
authors.initials: L
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hui-Yun
authors.initials: HY
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D002584:Cervix Uteri / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology