title: Elasticity in ionically cross-linked neurofilament networks.
abstract: Neurofilaments are found in abundance in the cytoskeleton of neurons, where they act as an intracellular framework protecting the neuron from external stresses. To elucidate the nature of the mechanical properties that provide this protection, we measure the linear and nonlinear viscoelastic properties of networks of neurofilaments. These networks are soft solids that exhibit dramatic strain stiffening above critical strains of 30-70%. Surprisingly, divalent ions such as Mg(2+), Ca(2+), and Zn(2+) act as effective cross-linkers for neurofilament networks, controlling their solidlike elastic response. This behavior is comparable to that of actin-binding proteins in reconstituted filamentous actin. We show that the elasticity of neurofilament networks is entropic in origin and is consistent with a model for cross-linked semiflexible networks, which we use to quantify the cross-linking by divalent ions.
authors.affiliation: Department of Physics, Harvard University, Cambridge, Massachusetts, USA. nyao@fas.harvard.edu
authors.forename: Norman Y
authors.initials: NY
authors.lastname: Yao
authors.forename: Chase P
authors.initials: CP
authors.lastname: Broedersz
authors.forename: Yi-Chia
authors.initials: YC
authors.lastname: Lin
authors.forename: Karen E
authors.initials: KE
authors.lastname: Kasza
authors.forename: Frederick C
authors.initials: FC
authors.lastname: Mackintosh
authors.forename: David A
authors.initials: DA
authors.lastname: Weitz
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology*; D000199:Actins / Q000502:physiology; D002118:Calcium / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology*; D004548:Elasticity / Q000502:physiology*; D054459:Elasticity Imaging Techniques / Q000379:methods; D008274:Magnesium / Q000494:pharmacology; D009415:Nerve Net / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D033081:Shear Strength / Q000502:physiology; D015032:Zinc / Q000494:pharmacology
chemical_list: D000199:Actins; D008274:Magnesium; D015032:Zinc; D002118:Calcium