Changes in antioxidant gene expression and induction of oxidative stress in pea (Pisum sativum L.) under Al stress.
Aluminium toxicity has been recognized as a primary growth-limiting factor in acid soil, resulting in a decrease in plant growth and production. In this experiment we have studied the induction of oxidative stress and changes in antioxidant gene expression in pea (var. ALASKA) under aluminium (Al) stress. We have found that Al treatment affected the growth of pea plant and induced oxidative stress with a change in antioxidant gene expression profile. While the expression of glutathione-s-transferase (GST) and catalase (CAT) was more in root, cytosolic Ascorbate peroxidase (cAPX) expression increased in shoot under aluminium stress. Copper- Zinc Superoxide dismutase (Cu-Zn SOD) gene expression was higher after 24 h but decreased after 48 h along with elevated expression of manganese superoxide dismutase (MnSOD) and iron-superoxide dismutase (FeSOD) at higher aluminium concentrations after 24 and 48 h. Aluminium stress elevated hydrogen peroxide (H(2)O(2)) level and affected the growth. The proline content did not change significantly, whereas glutathione content increased with a decreased ascorbate content under Al stress. The present study indicates that aluminium treatment affected the antioxidant gene expression and induced oxidative stress in pea plant.
Panda
Sanjib Kumar
SK
Plant Biochemistry and Molecular Biology Laboratory, School of Life Sciences, Assam University, Silchar 788011, India. drskp_au@yahoo.com
Matsumoto
Hideki
H
D000535:Aluminum / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism*; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000737:chemistry; D008969:Molecular Sequence Data; D016877:Oxidants / Q000737:chemistry; D018384:Oxidative Stress / Q000235:genetics*; D018532:Pisum sativum / Q000187:drug effects / Q000235:genetics / Q000502:physiology
D000975:Antioxidants; D004798:Enzymes; D016877:Oxidants; D006861:Hydrogen Peroxide; D000535:Aluminum