title: Antioxidative status and lipid peroxidation in kidney tissue of rats fed with vitamin B(6)-deficient diet.
abstract: BACKGROUND
  The aim of this study was to evaluate lipid peroxidation (LP) and free radical scavenging enzyme activities in kidney tissue of vitamin B(6)-deficient rats.
  
  
  METHODS
  The rats were divided into control and vitamin B(6)-deficient groups. After 4 weeks of feeding, animals in all groups were anesthetized by thiopental sodium (50 mg/kg). Thoraces were opened, 2 mL blood samples were taken from aortas, then the rats were killed by cervical dislocation, and kidney tissues were removed. Biochemical measurements in kidney tissue were carried out using a spectrophotometer.
  
  
  RESULTS
  Total superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), superoxide dismutase (SOD) activities, and antioxidant potential (AOP) values in the vitamin B(6)-deficient group were significantly lower than those of the control group, whereas glutathione peroxidase (GSH-Px), glutathione reductase (GRD), glutathione-S-transferase (GST) activities, and malondialdehyde (MDA) level were significantly higher than those of the control group (p < 0.05).
  
  
  CONCLUSIONS
  The results show that vitamin B(6) deficiency causes an attenuation in antioxidant defense system and an increase in oxidative stress in kidney tissue of rats.
authors.affiliation: Department of Internal Medicine, Division of Nephrology, School of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Keles
authors.forename: Behcet
authors.initials: B
authors.lastname: Al
authors.forename: Kenan
authors.initials: K
authors.lastname: Gumustekin
authors.forename: Berna
authors.initials: B
authors.lastname: Demircan
authors.forename: Isa
authors.initials: I
authors.lastname: Ozbey
authors.forename: Mehmet
authors.initials: M
authors.lastname: Akyuz
authors.forename: Adnan
authors.initials: A
authors.lastname: Yilmaz
authors.forename: Elif
authors.initials: E
authors.lastname: Demir
authors.forename: Abdullah
authors.initials: A
authors.lastname: Uyanik
authors.forename: Tevfik
authors.initials: T
authors.lastname: Ziypak
authors.forename: Seyithan
authors.initials: S
authors.lastname: Taysi
mesh_terms: D000818:Animals; D016166:Free Radical Scavengers / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D026681:Vitamin B 6 Deficiency / Q000378:metabolism*
chemical_list: D016166:Free Radical Scavengers