title: Pharmacologically separable [(3)H]glutamate binding sites on insect muscle membranes.
abstract: A high affinity, saturable, [(3)H]l-glutamate binding site has been described in a cockroach (Periplaneta americana) coxal muscle membrane preparation. This site exhibits a K(D) of 1.1 ?M and a B(max) of 15.5 pmol mg(?1) protein. The pharmacological profile is compatible with that expected of a functional l-glutamate receptor and there is good agreement with physiological data obtained for the insect neuromuscular junction in other laboratories. There appear to be at least two subpopulations of l-glutamate binding sites in cockroach muscle membranes which can be distinguished biochemically with the agonists ibotenate and quisqualate.
authors.affiliation: AFRC Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, Downing St, Cambridge CB2 3EJ, U.K.
authors.forename: M I
authors.initials: MI
authors.lastname: Sepúlveda
authors.forename: D B
authors.initials: DB
authors.lastname: Sattelle