A rabbit model of lower eyelid fibrosis.
OBJECTIVE
To create and validate a new model of lower eyelid fibrosis in Dutch-belted rabbits.


METHODS
Five Dutch-belted rabbits were injected with a transcutaneous 1-ml injection of standard 95% ethanol alcohol just inferior to the eyelid margin of one lower eyelid. A control injection of 1 ml of balanced saline solution was given to the opposite eyelid. A small tattoo was placed on the skin overlying the inferior orbital rim and used as a measuring point of reference in relation to the lower eyelid margin. Analysis was twofold: eyelid measurements were made over 8 weeks to determine the presence of eyelid shortening, and a histopathologic analysis was performed.


RESULTS
Mean lower eyelid shortening was greater in the ethanol alcohol intervention eyelids than the control group (-3.4 mm +/- 1.67 mm vs. 0.5 mm +/- 0.71 mm, p = 0.01). Histopathologic analysis revealed extensive fibrosis in the ethanol alcohol invention eyelids compared with the control group.


CONCLUSIONS
Ethanol alcohol induces eyelid fibrosis and lower eyelid shortening. This may be a useful model in the future testing of novel surgical or pharmacologic treatments.
Griepentrog
Gregory J
GJ
Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota 55905, USA. gigriepe@hotmail.com
Park
D J John
DJ
Zaldivar
Renzo A
RA
Pulido
Jose S
JS
Cameron
J Douglas
JD
Woog
John J
JJ
D000818:Animals; D004195:Disease Models, Animal; D000431:Ethanol / Q000633:toxicity; D005143:Eyelids / Q000187:drug effects / Q000473:pathology*; D005355:Fibrosis / Q000139:chemically induced; D008297:Male; D011817:Rabbits
D000431:Ethanol