Simultaneous determination of substrate and product in the process of preparation of valienamine by capillary zone electrophoresis.
A simple and rapid CZE method was established for the simultaneous determination of valienamine, acarbose and validamycin A, using a 20-kV CZE with the detection wavelength of 193 nm and 50 mM phosphoric acid-20 mM Tris (pH 5.3) as a running buffer. The calibration curves of valienamine, acarbose, and validamycin A showed a good linear relationship at a concentration range of 5-1000 microg/mL. The detection limits of valienamine, acarbose, and validamycin A were 0.3, 0.6, and 0.6 microg/mL, respectively, and the average recoveries of each of the above were 99.9, 99.5, and 100.3%. The method has been successfully applied for simultaneous determination of substrate and product in the process of preparation of valienamine.
Wei
Xiao-Dong
XD
Shanghai Institute of Pharmaceutical Industry, Shanghai, P. R. China. weixdong@hotmail.com
Zhao
Wen-Jie
WJ
Gu
Min
M
Zhao
Bo
B
Yao
Rong-Ying
RY
D020909:Acarbose / Q000032:analysis*; D053138:Cyclohexenes / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006595:Hexosamines / Q000032:analysis*; D007294:Inositol / Q000031:analogs & derivatives / Q000032:analysis; D015203:Reproducibility of Results
D053138:Cyclohexenes; D006595:Hexosamines; C030790:validamycin A; C030791:valienamine; D007294:Inositol; D020909:Acarbose