A diagnostic method for pneumocystis carinii a causative agent of pneumonia in immunodeficient rats.
Immunodeficient animals are in demand in current biomedical research, and they contribute to medical progress. In Pneumocystis infections, a specific histological diagnostic tool may be immunochemistry (IC). However, it was recently reported that the antibody (3F6) was not suitable for detecting Pneumocystis in rats. We purchased another antibody [PNC007] from a commercial source for IC. We could detect positive signals at identical locations with IC and Toluidine blue O in lungs of infected rats. These results corresponded to the results obtained with PCR. We should study the relationship between unexpected positive signals seen in IC and trophic forms in lungs of infected rats. We could clinically diagnose pneumonia caused by Pneumocystis carinii with the diagnostic method we developed.
Yabuuchi
Kaori
K
Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
Tajima
Masaru
M
Kotani
Yuko
Y
Aihara
Katsumi
K
Kawasaki
Aiko
A
Kaneko
Shiro
S
Takagi
Yasuhiro
Y
Kawai
Sumiko
S
Shioya
Kyoko
K
Okamoto
Akira
A
Kagiyama
Soichiro
S
Kurosawa
Tsutomu
T
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D005260:Female; D016867:Immunocompromised Host; D007150:Immunohistochemistry / Q000379:methods*; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D045363:Pneumocystis carinii / Q000276:immunology / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D051381:Rats; D011916:Rats, Inbred F344; D017208:Rats, Wistar
D000908:Antibodies, Fungal