title: Fluorescence/luminescence circadian imaging of complex tissues at single-cell resolution.
abstract: The use of luciferase reporter genes together with luminescence detection has enabled high frequency monitoring of molecular circadian clock function in living tissues. With the help of an intensified CCD camera combined with an inverted epifluorescence microscope, the authors have established a new imaging strategy that makes use of transgenic cell type-specific expression of fluorescent proteins to identify cells of interest for subsequent circadian luminescence recording at single-cell resolution.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Sellix
authors.forename: Jake
authors.initials: J
authors.lastname: Currie
authors.forename: Michael
authors.initials: M
authors.lastname: Menaker
authors.forename: Herman
authors.initials: H
authors.lastname: Wijnen
mesh_terms: D000818:Animals; D002468:Cell Physiological Phenomena; D002940:Circadian Rhythm / Q000235:genetics*; D004330:Drosophila; D005453:Fluorescence; D015870:Gene Expression; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics; D049449:Luminescence; D008163:Luminescent Measurements; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000502:physiology
chemical_list: D008156:Luciferases