title: On the disposition of phospholipids in freshly isolated myelin sheath preparations from bovine brain.
abstract: The extent of availability of the four major classes of phospholipids in freshly isolated bovine brain myelin has been studied using phospholipase C (Clostridium perfringens), phospholipase A (bee venom), sphingomyelase (Staphalococcus aureus) and trinitrobenzenesulphonate. The results have been compared with those of a previous study (Gwarsha et al., 1984). Taken together, the data suggest that 47-67% of the phosphatidylcholine, 45-66% of the ethanolamine-containing phosphoglycerides, 52-55% of the spingomyelin and 10-32% of the phosphatidylserine + phosphatidylinositol in the fresh myelin is available to these probes and hence may be located at the external surface of the membrane bilayer.
authors.affiliation: Department of Biology, University of York, Heslington, York Y01 5DD, U.K.
authors.forename: K
authors.initials: K
authors.lastname: Gwarsha
authors.forename: M G
authors.initials: MG
authors.lastname: Rumsby
authors.forename: C
authors.initials: C
authors.lastname: Little