Immunomodulatory activities and subacute toxicity of a novel β-glucan from Paenibacillus polymyxa JB115 in rats.
Subacute toxicity and immunopharmacological activities of β-glucan from P. polymyxa JB115 was evaluated in a 28-day feeding study in rats. The white blood cell count, red blood cell count, hematocrit, hemoglobin, thrombocytes (THR) and thrombocytocrit were significantly higher in male fed with β-glucan than control rats and the insignificant lower eosinophil count, mean corpuscular volume, mean cell hemoglobin and uninfected THR (uTHR) levels were observed in male whereas no marked changes in female rats. No other significant differences in serum chemistry and liver, kidney, and spleen weights were observed. The pathological changes and other abnormal indicators were not detected in urine. Female rats fed with diet supplemented with 0.01% β-glucan also showed marked increase in the percentage of blood cytotoxic T-lymphocytes compared to that of the control group while not significant differences in the percentage of blood B-lymphocytes. No adverse effects on general condition and behavior, growth, feed and water consumption and feed conversion efficiency were found. The results suggest that consumption of the novel β-1, 3/1, 6-glucan from P. polymyxa JB115 was not associated with any obvious toxic effects in rats, indicating its safety as a potential immunostimulant or as an adjuvant of some animal vaccines.
Chang
Zhi-Qiang
ZQ
College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
Reza
Md Ahsanur
MA
Lee
Joong-Su
JS
Gebru
Elias
E
Jang
Seung-Hee
SH
Choi
Myung-Jin
MJ
Lee
Seung-Jin
SJ
Damte
Dereje
D
Kim
Jong-Choon
JC
Park
Seung-Chun
SC
D000284:Administration, Oral; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005936:Glucans / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity*; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity*; D008297:Male; D056507:Paenibacillus / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D036401:Toxicity Tests, Chronic
D005936:Glucans; D007155:Immunologic Factors; C033671:epiglucan