Identification of a specific IgE-binding protein from narrow-leafed lupin (L. Angustifolius) seeds.
Since lupin has been introduced as a food ingredient on the market there are more and more reports concerning its allergenic properties. However, only few narrow-leafed lupin proteins have yet been characterized as specific IgE-binding molecules and identified. The aim of the study has been to find and identify the main narrow-leafed lupin globulins that bind to specific IgEs from the sera of lupin-allergic people. Isolated lupin globulins were subjected to immunoblotting with the sera from people who suffered from lupin allergy. Incubation with alpha-methyl-D-galactopyranoside was performed to eliminate possible binding of unspecific human IgEs. The proteins binding specific IgEs from lupin-allergic patients' sera were identified by means of mass spectrometry. Western blot analysis revealed 2 signals corresponding to lupin globulins that bound to specific IgEs from the sera of people allergic to lupin. The globulins were identified as conglutin-gamma and its smaller subunit. The results suggested that individuals that displayed lupin allergy symptoms reacted to conglutin-gamma.


CONCLUSIONS
The results of the study can contribute to identification of yet undetected allergens of narrow-leafed lupin. This, in turn, can make lupin-fortified products safer for the consumers.
Kłos
Patrycja
P
Dept. of General Pathology, Pomeranian Medical Univ., Al. Powstańców Wielkopolskich 72, Szczecin, Poland. patrycja.klos@gmail.com
Poreba
Elzbieta
E
Springer
Ewa
E
Lampart-Szczapa
Eleonora
E
Goździcka Józefiak
Anna
A
D000328:Adult; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005916:Globulins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000302:isolation & purification / Q000378:metabolism*; D031297:Lupinus / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
D002352:Carrier Proteins; D005916:Globulins; D010940:Plant Proteins; D007073:Immunoglobulin E