title: Improved techniques for a murine model of myocardial ischemia.
abstract: OBJECTIVE
  Our study investigated a rapid and reliable method of surgical occlusion of coronary vessels in mice. The study improves the chance of success in making a myocardial ischemia model in mice, and provides a novel method to a novice and laboratory with limited conditions.
  
  
  RESULTS
  Sixty mice were evenly divided into two groups, a modified group (oral intubation and self-made rib retractor) and a conventional group (tracheotomy and rib cutting). During the perioperative period, the success rate of model establishment and the survival rate of the mice in the modified group were significantly higher than those in the conventional group (P<0.01). Also, the status of the mice in the modified group after operation was better than that of the conventional group. Moreover, operation times in the modified group were significantly shorter than those of the conventional group (P< 0.01). The infarct size, as assessed using triphenyltetrazolium chloride staining, was similar between the two groups (P> 0.05).
  
  
  CONCLUSIONS
  This novel method is simple and efficient and can be conducted independently, enhancing the success rate of myocardial ischemic model establishment in mice.
authors.affiliation: Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Wei
authors.forename: Rong-Qing
authors.initials: RQ
authors.lastname: Zhang
authors.forename: Hai-Chang
authors.initials: HC
authors.lastname: Wang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004562:Electrocardiography; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D017202:Myocardial Ischemia / Q000601:surgery*; D016019:Survival Analysis