Fluorescence/luminescence circadian imaging of complex tissues at single-cell resolution.
The use of luciferase reporter genes together with luminescence detection has enabled high frequency monitoring of molecular circadian clock function in living tissues. With the help of an intensified CCD camera combined with an inverted epifluorescence microscope, the authors have established a new imaging strategy that makes use of transgenic cell type-specific expression of fluorescent proteins to identify cells of interest for subsequent circadian luminescence recording at single-cell resolution.
Sellix
Michael T
MT
Currie
Jake
J
Menaker
Michael
M
Wijnen
Herman
H
D000818:Animals; D002468:Cell Physiological Phenomena; D002940:Circadian Rhythm / Q000235:genetics*; D004330:Drosophila; D005453:Fluorescence; D015870:Gene Expression; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics; D049449:Luminescence; D008163:Luminescent Measurements; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000502:physiology
D008156:Luciferases