title: Control of oligodendroglial cell number by the miR-17-92 cluster.
abstract: The generation of myelinating cells in the central nervous system requires the initiation of specific gene expression programs in oligodendrocytes. We reasoned that microRNAs (miRNAs) could play an important role in this process by regulating crucial developmental genes. Microarray profiling of cultured oligodendrocytes identified the miR-17-92 miRNA cluster as highly enriched in oligodendrocytes. We specifically deleted the miR-17-92 cluster in oligodendrocytes using 2',3'-cyclic nucleotide 3' phosphodiesterase (Cnp)-Cre mice. Absence of miR-17-92 leads to a reduction in oligodendrocyte number in vivo and we find that the expression of these miRNAs in primary cultures of oligodendrocyte precursor cells promotes cell proliferation by influencing Akt signaling. Together, these results suggest that the miRNA pathway is essential in determining oligodendroglial cell number and that the miR-17-92 cluster is crucial in this process.
authors.affiliation: Max Planck Institute for Experimental Medicine, Hermann-Rein-Strasse 3, D-37075 Göttingen, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Budde
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schmitt
authors.forename: Dirk
authors.initials: D
authors.lastname: Fitzner
authors.forename: Lennart
authors.initials: L
authors.lastname: Opitz
authors.forename: Gabriela
authors.initials: G
authors.lastname: Salinas-Riester
authors.forename: Mikael
authors.initials: M
authors.lastname: Simons
mesh_terms: D000818:Animals; D002452:Cell Count; D049109:Cell Proliferation; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism; D043244:Ribonuclease III / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D043244:Ribonuclease III