title: Serum paraoxonase activity in patients with low glomerular filtration rates.
abstract: OBJECTIVE
  Epidemiological and experimental studies indicate that kidney disease is associated with increased oxidative stress. Our aim was to determine whether paraoxonase 1 (PON1) activity is altered in patients with moderately decreased glomerular filtration rates (GFRs) compared to healthy controls.
  
  
  METHODS
  Forty-eight patients showing relatively low GFRs upon renal scintigraphy with (99m)Tc-DTPA were compared to 40 age-matched healthy subjects. Serum PON1 activity was measured spectrophotometrically. Lipid hydroperoxide levels were measured via iodometric assay.
  
  
  RESULTS
  The mean ages of the patient and control groups were 32.09 +/- 6.10 (range 23-50) and 31.30 +/- 5.30 (range 20-46) years, respectively. Serum PON1 (p= 0.949) and high-density lipoprotein (p= 0.473) levels did not differ between groups. Significant differences were detected between groups in terms of mean triglyceride (p= 0.009), very-low-density lipoprotein (p = 0.010), lipid hydroperoxide (p = 0.026), urea (p = 0.012), and creatinine (p = 0.001) levels, whereas total cholesterol (p = 0.520) and low-density lipoprotein (p = 0.161) were similar between groups. Mean GFR was significantly lower in the low GFR group compared to the control (p = 0.000).
  
  
  CONCLUSIONS
  Our results indicate that PON1 activity and high-density lipoprotein levels may not be determining factors in premature vascular aging in patients with moderately decreased GFRs. Instead, some other undetermined factor(s) may be involved in modulating enzymatic activity.
authors.affiliation: Department of Urology, Faculty of Medicine, Harran University, Sanliurfa, Turkey. halilciftci63@hotmail.com
authors.forename: Halil
authors.initials: H
authors.lastname: Ciftci
authors.forename: Murat
authors.initials: M
authors.lastname: Savas
authors.forename: Ercan
authors.initials: E
authors.lastname: Yeni
authors.forename: Ayhan
authors.initials: A
authors.lastname: Verit
authors.forename: Hakim
authors.initials: H
authors.lastname: Celik
authors.forename: Halil
authors.initials: H
authors.lastname: Oncel
mesh_terms: D000328:Adult; D043303:Aryldialkylphosphatase / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D051437:Renal Insufficiency / Q000201:enzymology*; D055815:Young Adult
chemical_list: D008054:Lipid Peroxides; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human