Effects of aflatoxin B(1) and fumonisin B(1) on the viability and induction of apoptosis in rat primary hepatocytes.
The present study evaluated the effect of aflatoxin B(1) (AFB(1)) and fumonisin B(1) (FB(1)) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel electrophoresis and acridine orange and ethidium bromide staining. At the concentrations of AFB(1) and FB(1) used, the toxins did not decrease cell viability, but did induce apoptosis in a concentration and time-dependent manner.
Ribeiro
Deise H B
DH
Centro de P&D de Proteção Ambiental, Instituto Biológico, São Paulo, SP, Brazil.
Ferreira
Fabiane L
FL
da Silva
Valéria N
VN
Aquino
Simone
S
Corrêa
Benedito
B
D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D037341:Fumonisins / Q000633:toxicity*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D018613:Microscopy, Confocal; D051381:Rats; D017208:Rats, Wistar
D037341:Fumonisins; C056933:fumonisin B1; D016604:Aflatoxin B1