title: Mitoxantrone loaded superparamagnetic nanoparticles for drug targeting: a versatile and sensitive method for quantification of drug enrichment in rabbit tissues using HPLC-UV.
abstract: In medicine, superparamagnetic nanoparticles bound to chemotherapeutics are currently investigated for their feasibility in local tumor therapy. After intraarterial application, these particles can be accumulated in the targeted area by an external magnetic field to increase the drug concentration in the region of interest (Magnetic-Drug-Targeting). We here present an analytical method (HPLC-UV), to detect pure or ferrofluid-bound mitoxantrone in a complex matrix even in trace amounts in order to perform biodistribution studies. Mitoxantrone could be extracted in high yields from different tissues. Recovery of mitoxantrone in liver tissue (5000 ng/g) was 76 +/- 2%. The limit of quantification of mitoxantrone standard was 10 ng/mL +/-12%. Validation criteria such as linearity, precision, and stability were evaluated in ranges achieving the FDA requirements. As shown for pilot samples, biodistribution studies can easily be performed after application of pure or ferrofluid-bound mitoxantrone.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Friedrich-Alexander University Erlangen-Nürnberg, Section for Experimental Oncology and Nanomedicine (Else Kröner-Fresenius-Foundation-Professorship), 91054 Erlangen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Tietze
authors.forename: Eveline
authors.initials: E
authors.lastname: Schreiber
authors.forename: Stefan
authors.initials: S
authors.lastname: Lyer
authors.forename: Christoph
authors.initials: C
authors.lastname: Alexiou
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D016503:Drug Delivery Systems; D004355:Drug Stability; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008942:Mitoxantrone / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D009132:Muscles / Q000737:chemistry; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D010865:Pilot Projects; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents; D013552:Swine; D014018:Tissue Distribution
chemical_list: D012997:Solvents; D008942:Mitoxantrone; D052203:Ferrosoferric Oxide