Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics.
We apply a wide-field quantitative phase microscopy technique based on parallel two-step phase-shifting on-axis interferometry to visualize live biological cells and microorganism dynamics. The parallel on-axis holographic approach is more efficient with camera spatial bandwidth consumption compared to previous off-axis approaches and thus can capture finer sample spatial details, given a limited spatial bandwidth of a specific digital camera. Additionally, due to the parallel acquisition mechanism, the approach is suitable for visualizing rapid dynamic processes, permitting an interferometric acquisition rate equal to the camera frame rate. The method is demonstrated experimentally through phase microscopy of neurons and unicellular microorganisms.
Shaked
Natan T
NT
1Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University,Durham, North Carolina 27708, USA. natan.shaked@duke.edu
Newpher
Thomas M
TM
Ehlers
Michael D
MD
Wax
Adam
A
D000818:Animals; D002478:Cells, Cultured; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005056:Euglena gracilis / Q000166:cytology*; D006696:Holography / Q000295:instrumentation*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation*; D009474:Neurons / Q000166:cytology*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity