title: Association of resistance to activated protein with the presence of Leiden and Cambridge Factor V mutations in Mexican patients with primary thrombophilia.
abstract: BACKGROUND
  Leiden and Cambridge factor V coagulation mutations and activated protein C resistance (RaPC) are alterations related with vein and artery thrombosis. In this study we aimed to determine whether RaPC is associated with the presence of Leiden and Cambridge mutation and the frequency of these mutations in the racially mestizo Mexican population.
  
  
  METHODS
  We included 150 Mexican patients with primary thrombophilia and 100 healthy subjects in this study. RaPC was determined using commercial methods and genotypes FV Leiden and FV Cambridge with PCR-RFLPs.
  
  
  RESULTS
  RaPC was positive in four patients and in one control individual; however, there was no presence of Leiden or Cambridge mutation in the studied group; thus, RaPC was not correlated with the presence of any of the studied mutations.
  
  
  CONCLUSIONS
  These results indicate that there are other primary or secondary causes different from those studied, which condition the presence of RaPC. Furthermore, the frequency obtained for RaPC in our thrombophilic population of racially mixed Mexicans is lower compared to that obtained in the Caucasian population, most probably because they are genetically different populations.
authors.affiliation: Laboratorio Central de Patología Clínica, Instituto Nacional de Rehabilitación, México, DF, Mexico.
authors.forename: César
authors.initials: C
authors.lastname: Zavala Hernández
authors.forename: Edgar
authors.initials: E
authors.lastname: Hernández Zamora
authors.forename: Carlos
authors.initials: C
authors.lastname: Martínez Murillo
authors.forename: María de la Luz
authors.initials: Mde L
authors.lastname: Arenas Sordo
authors.forename: Ana Elena
authors.initials: AE
authors.lastname: González Orozco
authors.forename: Elba
authors.initials: E
authors.lastname: Reyes Maldonado
mesh_terms: D020016:Activated Protein C Resistance / Q000235:genetics*; D000328:Adult; D005165:Factor V / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008800:Mexico; D009154:Mutation; D011446:Prospective Studies; D019851:Thrombophilia / Q000235:genetics*
chemical_list: C119012:factor V Cambridge; C095381:factor V Leiden; D005165:Factor V