Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of the gallbladder.
OBJECTIVE
Establish the prevalence of Helicobacter spp in chronic cholecystitis and its correlation with the gallbladder's histological findings.


METHODS
100 patients were operated for chronic cholecystitis with cholecystolithiasis. In pathological examination of the gallbladder, were evaluated the presence of metaplasia, dysplasia, lymphoid follicles, anaplasia and tumors that might be related to the presence of Helicobacter plus the presence of the bacilli Giemsa(+) by optical microscopy. From the DNA extracted from the gallbladder's bile, PCR was performed by using specific primers for the identification of Helicobacter spp with amplification of the 400bp segment of rRNA gene16S, with positive control DNA from Helicobacter pylori. All the cases negative for isolation of genetic material were excluded. The cases of PCRTheta and GiemsaTheta were used as negative control group. The histological findings were compared to the presence of bacilli and PCR data using a chi-square and Fisher's Exact test (CI = 95.0%, p <0.05).


RESULTS
Of 68 patients, 42 (61.8%) were PCR(+) for Helicobacter spp and 19 (27.9%) had Giemsa(+). There was no correlation between the two findings. The PCR(+) for Helicobacter spp was not correlated to the histological findings. The presence of lymphoid follicles and metaplasia was related to the Giemsa(+) (p = 0.025 and p= 0.039).


CONCLUSIONS
There is high prevalence of Helicobacter spp in patients with chronic cholecystitis and cholecystolithiasis without be correlated with the histological patterns studied.
Moricz
André de
Ad
Department of Surgery, Santa Casa School of Medicine, São Paulo, Brazil. amoricz@uol.com.br <amoricz@uol.com.br>
Melo
Murilo
M
Castro
Ana Maria
AM
Campos
Tercio
T
Silva
Rodrigo Altenfelder
RA
Pacheco
Adhemar Monteiro
AM
D000328:Adult; D000368:Aged; D016009:Chi-Square Distribution; D002764:Cholecystitis / Q000382:microbiology* / Q000473:pathology; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005704:Gallbladder / Q000382:microbiology / Q000473:pathology; D042882:Gallstones / Q000473:pathology; D016998:Helicobacter / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D055815:Young Adult
D004269:DNA, Bacterial