title: Genome-wide DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) residing in mouse pluripotent stem cells.
abstract: DNA methylation profile, consisting of tissue-dependent and differentially methylated regions (T-DMRs), has elucidated tissue-specific gene function in mouse tissues. Here, we identified and profiled thousands of T-DMRs in embryonic stem cells (ESCs), embryonic germ cells (EGCs) and induced pluripotent stem cells (iPSCs). T-DMRs of ESCs compared with somatic tissues well illustrated gene function of ESCs, by hypomethylation at genes associated with CpG islands and nuclear events including transcriptional regulation network of ESCs, and by hypermethylation at genes for tissue-specific function. These T-DMRs in EGCs and iPSCs showed DNA methylation similar to ESCs. iPSCs, however, showed hypomethylation at a considerable number of T-DMRs that were hypermethylated in ESCs, suggesting existence of traceable progenitor epigenetic information. Thus, DNA methylation profile of T-DMRs contributes to the mechanism of pluripotency, and can be a feasible solution for identification and evaluation of the pluripotent cells.
authors.affiliation: Laboratory of Cellular Biochemistry, Department of Animal Resource Sciences/Veterinary Medical Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Sato
authors.forename: Shintaro
authors.initials: S
authors.lastname: Yagi
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Arai
authors.forename: Keiji
authors.initials: K
authors.lastname: Hirabayashi
authors.forename: Naoko
authors.initials: N
authors.lastname: Hattori
authors.forename: Misa
authors.initials: M
authors.lastname: Iwatani
authors.forename: Keisuke
authors.initials: K
authors.lastname: Okita
authors.forename: Jun
authors.initials: J
authors.lastname: Ohgane
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tanaka
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Wakayama
authors.forename: Shinya
authors.initials: S
authors.lastname: Yamanaka
authors.forename: Kunio
authors.initials: K
authors.lastname: Shiota
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D053595:Embryonic Stem Cells / Q000166:cytology; D020869:Gene Expression Profiling / Q000379:methods*; D016678:Genome / Q000235:genetics*; D051379:Mice; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D039904:Pluripotent Stem Cells / Q000378:metabolism*; D016133:Polymerase Chain Reaction