Identification of the novel dfrK-carrying transposon Tn559 in a porcine methicillin-susceptible Staphylococcus aureus ST398 strain.
The trimethoprim resistance gene dfrK was found to be part of the novel Tn554-related transposon Tn559 integrated in the chromosomal radC gene of a porcine methicillin-susceptible Staphylococcus aureus ST398 strain. While Tn559 and Tn554 had similar arrangements of the transposase genes tnpA, tnpB, and tnpC, the Tn554-associated resistance genes erm(A) and spc were replaced by dfrK in Tn559. Circular forms of Tn559 were detected and suggest the functional activity of this transposon.
Kadlec
Kristina
K
Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Höltystr. 10, 31535 Neustadt-Mariensee, Germany.
Schwarz
Stefan
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D005260:Female; D008712:Methicillin / Q000494:pharmacology*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000382:microbiology*; D014296:Trimethoprim Resistance / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008712:Methicillin