title: Expansion of genes encoding a novel type of dynamin in the genome of the pea aphid, Acyrthosiphon pisum.
abstract: Screening of the entire genome of the pea aphid, Acyrthosiphon pisum, detected 15 genes for putative dynamin superfamily proteins - self-assembling large GTPases that are involved in the fission and fusion of membranes. In addition to a single gene each for Dyn, Drp1, and Opa1, orthologues that are common in Metazoa, 12 genes encoding a novel type of dynamin were found. Phylogenetic analyses showed that these novel-class genes are monophyletic. Quantitative reverse transcription-PCR demonstrated that expressions of four novel-class dynamin genes are highly up-regulated in the midgut, through which aphids take in phloem-sap diets and plant viruses. As this type of dynamin is absent from all other fully sequenced organisms, they may function in processes unique to aphids.
authors.affiliation: Advanced Science Institute, RIKEN, Wako, Saitama, Japan. bachi@riken.jp <bachi@riken.jp>
authors.forename: A
authors.initials: A
authors.lastname: Nakabachi
authors.forename: S
authors.initials: S
authors.lastname: Miyagishima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000201:enzymology / Q000235:genetics* / Q000821:virology; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D034281:Dynamins / Q000235:genetics*; D015870:Gene Expression; D017344:Genes, Insect; D049750:Genome, Insect; D019476:Insect Proteins / Q000235:genetics*; D007303:Insect Vectors / Q000821:virology; D008969:Molecular Sequence Data; D005810:Multigene Family; D018532:Pisum sativum / Q000469:parasitology / Q000821:virology; D010802:Phylogeny; D010942:Plant Viruses; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D019476:Insect Proteins; D034281:Dynamins