Structural signatures of antibiotic binding sites on the ribosome.
The ribosome represents a major target for antibacterial drugs. Being a complex molecular machine, it offers many potential sites for functional interference. The high-resolution structures of ribosome in complex with various antibiotics provide a unique data set for understanding the universal features of drug-binding pockets on the ribosome. In this work, we have analyzed the structural and evolutionary properties of 65 antibiotic binding sites (ABSs) in the ribosome. We compared these sites to similar-size computed pockets extracted from the small and large ribosomal subunits. Based on this analysis, we defined properties of the known drug-binding sites, which constitute the signature of a 'druggable' site. The most noticeable properties of the ABSs are prevalence of non-paired bases, a strong bias in favor of unusual syn conformation of the RNA bases and an unusual sugar pucker. We propose that despite the different geometric and chemical properties of diverse antibiotics, their binding sites tend to have common attributes that possibly reflect the potency of the pocket for binding small molecules. Finally, we utilized the ensemble of properties to derive a druggability index, which can be used in conjunction with site functionality information to identify new drug-binding sites on the ribosome.
David-Eden
Hilda
H
Faculty of Biology, Technion - Israel Institute of Technology, Haifa 32000, Israel.
Mankin
Alexander S
AS
Mandel-Gutfreund
Yael
Y
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001665:Binding Sites; D015195:Drug Design; D019143:Evolution, Molecular; D008024:Ligands; D008958:Models, Molecular; D012335:RNA, Ribosomal / Q000737:chemistry; D054681:Ribosome Subunits, Large, Bacterial / Q000737:chemistry*; D054680:Ribosome Subunits, Small, Bacterial / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D008024:Ligands; D012335:RNA, Ribosomal