Lipase immobilized by modification-coupled and adsorption-cross-linking methods: A comparative study.
Candida antarctica lipase was immobilized by an adsorption and cross-linking method with NW-ZT2 and by modification-coupled method with a silica-PEG gel. The final product silica-PEG-lipase was confirmed by IR spectra. The optimum pH value, the optimum temperature, the thermo-stabilities and operational stabilities for two kinds of immobilized lipase were also determined. Results show that the silica-PEG-lipase gel was superior to the lipase immobilized by adsorption and cross-linking, however both are viable for use in transesterification reactions.
Yang
Jianjun
J
School of Chemical Engineering, Northwest University, 710069, Xi'an, China.
Ma
Xiaoxun
X
Zhang
Zisheng
Z
Chen
Bin
B
Li
Shuang
S
Wang
Guijun
G
D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology; D003432:Cross-Linking Reagents; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005656:Fungal Proteins; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D013055:Spectrophotometry, Infrared; D013696:Temperature
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D008049:Lipase; C431349:lipase B, Candida antarctica