Fluorescent determination of Hg2+ in water and fish samples using a chemodosimeter based in a Rhodamine 6G derivative and a portable fiber-optic spectrofluorimeter.
A fluorimetric chemodosimeter (FC1), based on a Rhodamine 6G derivative, is proposed for the recognition of Hg(2+) ions in water and fish samples. The reagent shows a highly selective and sensitive reaction with Hg(2+), giving rise to strong fluorescence emission at 555 nm. The obvious color change of the solution from colorless to pink upon the addition of Hg(2+) demonstrates that FC1 can be used for "naked-eye" detection of Hg(2+) in water effluents. The fluorescence intensity is proportional to the amount of Hg(2+) at ng mL(-1) levels, and it is capable of distinguishing between safe and toxic levels of inorganic mercury in drinking water and fish samples. The procedure has been implemented in a portable instrument composed of a 515 nm light-emitting diode (LED) excitation source, two fiber optics, and a charge-coupled device (CCD) camera as detector, connected to a portable computer for data acquisition and analysis, intended for in situ determination of mercury, offering a viable alternative to a conventional spectrofluorimeter. The proposed method has been applied to different water and fish samples with satisfactory results.
Bohoyo Gil
Diego
D
Technological Institute of Food and Agriculture (INTAEX), 06071 Badajoz, Spain. dbohoyo@unex.es
Rodriguez-Cáceres
María Isabel
MI
Hurtado-Sánchez
María del Carmen
Mdel C
Muñoz de la Peña
Arsenio
A