title: [(3)H]GABA binding in the cerebellum of the reeler murine mutant.
abstract: In the cerebellum of the reeler mutant mouse, characterized morphologically by depletion of the granule cell population and abnormal synapse formation, increased GABA concentration and alterations in [(3)H]GABA binding have been observed. This study shows decreased affinity of the Na(+)-independent, high affinity GABA binding component of synaptosomal membranes and an increased affinity of the Na(+)-dependent, high affinity GABA binding component in reeler cerebellar homogenate and synaptic membranes. In contrast to the changes in affinity, the number of both Na(+)-dependent and Na(+)-independent binding sites was not significantly altered. The decreased affinity of the Na(+)-independent GABA binding and the increased affinity of the Na(+)-dependent binding, evidenced only in cerebellar tissue, were interpreted to indicate, respectively, hypo- and hypersensitivity of the postsynaptic and presynaptic elements of cerebellar GABAergic synapses, induced by the depressed excitatory granule cell input and/or the increased mossy fiber contact with the ectopic Purkinje cells.
authors.affiliation: Laboratory of Human and Animal Physiology, Department of Biology, University of Patra, Patra, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Matsokis
authors.forename: T
authors.initials: T
authors.lastname: Valcana