Putative Arabidopsis transcriptional adaptor protein (PROPORZ1) is required to modulate histone acetylation in response to auxin.
Plant development is highly adaptable and controlled by a combination of various regulatory circuits that integrate internal and environmental cues. The phytohormone auxin mediates such growth responses, acting as a dynamic signal in the control of morphogenesis via coordinating the interplay between cell cycle progression and cell differentiation. Mutants in the chromatin-remodeling component PROPORZ1 (PRZ1; also known as AtADA2b) are impaired in auxin effects on morphogenesis, suggestive of an involvement of PRZ1-dependent control of chromatin architecture in the determination of hormone responses. Here we demonstrate that PRZ1 is required for accurate histone acetylation at auxin-controlled loci. Specifically, PRZ1 is involved in the modulation of histone modifications and corresponding adjustments in gene expression of Arabidopsis KIP RELATED PROTEIN (KRP) CDK inhibitor genes in response to auxin. Deregulated KRP expression in KRP silencer lines phenocopies prz1 hyperproliferative growth phenotypes, whereas in a KRP overexpression background some mutant phenotypes are suppressed. Collectively, our findings support a model in which translation of positional signals into developmental cues involves adjustments in chromatin modifications that orchestrate auxin effects on cell proliferation.
Anzola
Jeanette Moulinier
JM
Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences (BOKU), A-1190 Vienna, Austria.
Sieberer
Tobias
T
Ortbauer
Martina
M
Butt
Haroon
H
Korbei
Barbara
B
Weinhofer
Isabelle
I
Müllner
Almuth Elise
AE
Luschnig
Christian
C
D000107:Acetylation; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D030821:Plants, Genetically Modified; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
C474635:ADA2b protein, Arabidopsis; D029681:Arabidopsis Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D017931:DNA Primers; D018744:DNA, Plant; D006657:Histones; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D014157:Transcription Factors