Single-file diffusion of protein drugs through cylindrical nanochannels.
A new drug delivery device using cylindrical block copolymer nanochannels was successfully developed for controlled protein drug delivery applications. Depending on the hydrodynamic diameter of the protein drugs, the pore size in cylindrical nanochannels could be controlled precisely down to 6 nm by Au deposition. Zero-order release of bovine serum albumin (BSA) and human growth hormone (hGH) by single-file diffusion, which has been observed for gas diffusion through zeolite pores, was realized up to 2 months without protein denaturation. Furthermore, a nearly constant in vivo release of hGH from the drug delivery nanodevice implanted to Sprague-Dawley (SD) rats was continued up to 3 weeks, demonstrating the feasibility for long-term controlled delivery of therapeutic protein drugs.
Yang
Seung Yun
SY
National Creative Research Center for Block Copolymer Self-Assembly, Department of Environmental Science & Engineering, Pohang University of Science and Technology, Kyungbuk, Korea.
Yang
Jeong-A
JA
Kim
Eung-Sam
ES
Jeon
Gumhye
G
Oh
Eun Ju
EJ
Choi
Kwan Yong
KY
Hahn
Sei Kwang
SK
Kim
Jin Kon
JK
D000818:Animals; D002417:Cattle; D004058:Diffusion; D016503:Drug Delivery Systems / Q000379:methods*; D006046:Gold / Q000737:chemistry; D019382:Human Growth Hormone / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D007267:Injections; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D016062:Porosity; D011506:Proteins / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000737:chemistry
D008567:Membranes, Artificial; D004364:Pharmaceutical Preparations; D011137:Polystyrenes; D011506:Proteins; D019382:Human Growth Hormone; D012710:Serum Albumin, Bovine; D006046:Gold; D019904:Polymethyl Methacrylate