title: The capsid protein encoded by U(L)17 of herpes simplex virus 1 interacts with tegument protein VP13/14.
abstract: The U(L)17 protein (pU(L)17) of herpes simplex virus 1 (HSV-1) likely associates with the surfaces of DNA-containing capsids in a heterodimer with pU(L)25. pU(L)17 is also associated with viral light particles that lack capsid proteins, suggesting its presence in the tegument of the HSV-1 virion. To help determine how pU(L)17 becomes incorporated into virions and its functions therein, we identified pU(L)17-interacting proteins by immunoprecipitation with pU(L)17-specific IgY at 16 h postinfection, followed by mass spectrometry. Coimmunoprecipitated proteins included cellular histone proteins H2A, H3, and H4; the intermediate filament protein vimentin; the major HSV-1 capsid protein VP5; and the HSV tegument proteins VP11/12 (pU(L)46) and VP13/14 (pU(L)47). The pU(L)17-VP13/14 interaction was confirmed by coimmunoprecipitation in the presence and absence of intact capsids and by affinity copurification of pU(L)17 and VP13/14 from lysates of cells infected with a recombinant virus encoding His-tagged pU(L)17. pU(L)17 and VP13/14-HA colocalized in the nuclear replication compartment, in the cytoplasm, and at the plasma membrane between 9 and 18 h postinfection. One possible explanation of these data is that pU(L)17 links the external face of the capsid to VP13/14 and associated tegument components.
authors.affiliation: Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853, USA.
authors.forename: Luella D
authors.initials: LD
authors.lastname: Scholtes
authors.forename: Kui
authors.initials: K
authors.lastname: Yang
authors.forename: Lucy X
authors.initials: LX
authors.lastname: Li
authors.forename: Joel D
authors.initials: JD
authors.lastname: Baines
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D002846:Chromatography, Affinity; D003593:Cytoplasm / Q000737:chemistry; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D011485:Protein Binding; D025941:Protein Interaction Mapping; D014760:Viral Fusion Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: C085822:VP13-14 protein, herpes simplex virus type 1; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C069185:DNA cleavage and packaging proteins, Herpesvirus