Fluorescent ratiometric MFC probe sensitive to early stages of alpha-synuclein aggregation.
We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of alpha-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of alpha-synuclein aggregation, a process associated with Parkinson's disease.
Yushchenko
Dmytro A
DA
Laboratory of Cellular Dynamics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
Fauerbach
Jonathan A
JA
Thirunavukkuarasu
Shyamala
S
Jares-Erijman
Elizabeth A
EA
Jovin
Thomas M
TM
D005456:Fluorescent Dyes / Q000737:chemistry*; D008301:Maleimides / Q000737:chemistry*; D008958:Models, Molecular; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013997:Time Factors; D051844:alpha-Synuclein / Q000737:chemistry* / Q000378:metabolism*
D005456:Fluorescent Dyes; D008301:Maleimides; D013438:Sulfhydryl Compounds; D051844:alpha-Synuclein