Maize 27 kDa gamma-zein is a potential allergen for early weaned pigs.
Soybean and maize are extensively used in animal feed, primarily in poultry, swine, and cattle diets. Soybean meal can affect pig performance in the first few weeks following weaning and elicit specific antibodies in weaned piglets. Though maize is a major component of pig feed, it is not known if any of the maize proteins can elicit immunological response in young pigs. In this study, we have identified a prominent 27 kDa protein from maize as an immunodominant protein in young pigs. This protein, like some known allergens, exhibited resistance to pepsin digestion in vitro. Several lines of evidence identify the immunodominant 27 kDa protein as a gamma-zein, a maize seed storage protein. First, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of different solubility classes of maize seed proteins revealed the presence of an abundant 27 kDa protein in the prolamin (zein) fraction. Antibodies raised against the purified maize 27 kDa gamma-zein also reacted against the same protein recognized by the young pig serum. Additionally, matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis of the peptides generated by trypsin digestion of the immunodominant 27 kDa protein showed significant homology to the maize 27 kDa gamma-zein. Since eliminating the allergenic protein will have a great impact on the nutritive value of the maize meal and expand its use in the livestock industry, it will be highly desirable to develop maize cultivars completely lacking the 27 kDa allergenic protein.
Krishnan
Hari B
HB
U.S. Department of Agriculture-Agricultural Research Service, Plant Genetics Research Unit, 205 Curtis Hall, University of Missouri, Columbia, Missouri 65211, USA. Hari.Krishnan@ARS.USDA.GOV
Kerley
Monty S
MS
Allee
Gary L
GL
Jang
Sungchan
S
Kim
Won-Seok
WS
Fu
Chunjiang J
CJ
D000485:Allergens / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D000595:Amino Acid Sequence; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary*; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013552:Swine / Q000276:immunology* / Q000502:physiology; D014886:Weaning; D003313:Zea mays / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D015028:Zein / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
D000485:Allergens; D015028:Zein