Production of butyl-biodiesel using lipase physically-adsorbed onto electrospun polyacrylonitrile fibers.
Butyl-biodiesel production using electrospun polyacrylonitrile fibers with Pseudomonas cepacia lipase immobilized through physical adsorption was studied. About 80% conversion to butyl-biodiesel was achieved after 24h by suspending the catalyst at 2.4 mg/mL in a mixture of rapeseed oil and n-butanol at a molar ratio of 1:3, containing water at 8000 ppm at 40 degrees C. A further 24h of operation resulted in 94% conversion. The initial reaction rate detected for this process was 65-fold faster than those detected for Novozym 435 on a total catalyst mass basis. The immobilized lipase continued to work as a catalyst for 27 d, within a 15% reduction in conversion yield at the outlet of the reactor compared with the average value detected during the first 3d of operation in a continuous butyl-biodiesel production system.
Sakai
Shinji
S
Division of Chemical Engineering, Department of Materials Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan. sakai@cheng.es.osaka-u.ac.jp
Liu
Yuping
Y
Yamaguchi
Tetsu
T
Watanabe
Rie
R
Kawabe
Masaaki
M
Kawakami
Koei
K
D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D056804:Biofuels / Q000032:analysis*; D001709:Biotechnology / Q000379:methods*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005656:Fungal Proteins; D008049:Lipase / Q000378:metabolism*; D013696:Temperature; D014867:Water / Q000737:chemistry
D000180:Acrylic Resins; D056804:Biofuels; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D014867:Water; C010504:polyacrylonitrile; C093667:Novozyme 435; D008049:Lipase