Chemical models and their mechanistic implications for the transformation of 6-cyanouridine 5'-monophosphate catalyzed by orotidine 5'-monophosphate decarboxylase.
The reactions of 6-cyano-1,3-dimethyluracil have been studied as chemical models to illustrate the mechanism for the transformation of 6-cyanouridine 5'-monophosphate (6-CN-UMP) to barbiturate ribonucleoside 5'-monophosphate (BMP) catalyzed by orotidine 5'-monophosphate decarboxylase (ODCase). The results suggest that the Asp residue in the ODCase active site plays the role of a general base in the transformation.
Wu
Yuen-Jen
YJ
Department of Chemistry, National Taiwan Normal University, No.88, Sec.4, Ting-Zhou Road, Taipei, 11677, Taiwan.
Liao
Chen-Chieh
CC
Jen
Cheng-Hung
CH
Shih
Yu-Chiao
YC
Chien
Tun-Cheng
TC
D001463:Barbiturates / Q000737:chemistry; D055162:Biocatalysis; D020134:Catalytic Domain; D008956:Models, Chemical; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000737:chemistry / Q000378:metabolism*; D014542:Uridine Monophosphate / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism
C513367:6-cyanouridine 5'-O-monophosphate; C541623:6-hydroxyuridine 5'-monophosphate; D001463:Barbiturates; D014542:Uridine Monophosphate; D009964:Orotidine-5'-Phosphate Decarboxylase