title: An environment-sensitive synthetic microbial ecosystem.
abstract: Microbial ecosystems have been widely used in industrial production, but the inter-relationships of organisms within them haven't been completely clarified due to complex composition and structure of natural microbial ecosystems. So it is challenging for ecologists to get deep insights on how ecosystems function and interplay with surrounding environments. But the recent progresses in synthetic biology show that construction of artificial ecosystems where relationships of species are comparatively clear could help us further uncover the meadow of those tiny societies. By using two quorum-sensing signal transduction circuits, this research designed, simulated and constructed a synthetic ecosystem where various population dynamics formed by changing environmental factors. Coherent experimental data and mathematical simulation in our study show that different antibiotics levels and initial cell densities can result in correlated population dynamics such as extinction, obligatory mutualism, facultative mutualism and commensalism. This synthetic ecosystem provides valuable information for addressing questions in ecology and may act as a chassis for construction of more complex microbial ecosystems.
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Hu
authors.forename: Jin
authors.initials: J
authors.lastname: Du
authors.forename: Rui-yang
authors.initials: RY
authors.lastname: Zou
authors.forename: Ying-jin
authors.initials: YJ
authors.lastname: Yuan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D017753:Ecosystem; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D053038:Quorum Sensing / Q000187:drug effects; D013559:Symbiosis / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents