Progress of liquid chromatography-mass spectrometry in measurement of vitamin D metabolites and analogues.
OBJECTIVE
Clinical testing for vitamin D nutritional status has experienced tremendous growth in the past several years, driven by research results linking various diseases with low serum 25-hydroxyvitamin D [25(OH)D] levels. Meanwhile, interest in the pathophysiological mechanism elucidation and pharmaceutical applications requires measurement of vitamin D metabolites and analogues. Liquid chromatography-mass spectrometry (LC-MS) has been increasingly utilized in these applications. In this work, our objective was to critically review the progress of LC-MS application in measuring vitamin D metabolites and analogues in biological fluids.


METHODS
The LC-MS methods included were selected from those searchable in PubMed up to January 2010.


CONCLUSIONS
LC-MS has unique advantages in measuring various vitamin D metabolites and analogues due to its flexibility, sensitivity, and specificity. Despite some controversies over serum 25(OH)D tests, LC-MS will be used for standardizing serum 25(OH)D assays using reference materials available from the National Institute of Standards and Technology.
El-Khoury
Joe M
JM
Department of Clinical Pathology, Cleveland Clinic, OH 44195, USA.
Reineks
Edmunds Z
EZ
Wang
Sihe
S
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000032:analysis* / Q000378:metabolism
D014807:Vitamin D; C104450:25-hydroxyvitamin D