title: Fructose production by inulinase covalently immobilized on sepabeads in batch and fluidized bed bioreactor.
abstract: The present work is an experimental study of the performance of a recently designed immobilized enzyme: inulinase from Aspergillus sp. covalently immobilized on Sepabeads. The aim of the work is to test the new biocatalyst in conditions of industrial interest and to assess the feasibility of the process in a fluidized bed bioreactor (FBBR). The catalyst was first tested in a batch reactor at standard conditions and in various sets of conditions of interest for the process. Once the response of the catalyst to different operating conditions was tested and the operational stability assessed, one of the sets of conditions tested in batch was chosen for tests in FBBR. Prior to reaction tests, preliminary fluidization tests were realized in order to define an operating range of admissible flow rates. As a result, the FBR was run at different feed flow rates in a closed cycle configuration and its performance was compared to that of the batch system. The FBBR proved to be performing and suitable for scale up to large fructose production.
authors.affiliation: Department of Engineering Modeling, University of Calabria, CS, Italy. ericca@unical.it <ericca@unical.it>
authors.forename: Emanuele
authors.initials: E
authors.lastname: Ricca
authors.forename: Vincenza
authors.initials: V
authors.lastname: Calabrò
authors.forename: Stefano
authors.initials: S
authors.lastname: Curcio
authors.forename: Alessandra
authors.initials: A
authors.lastname: Basso
authors.forename: Lucia
authors.initials: L
authors.lastname: Gardossi
authors.forename: Gabriele
authors.initials: G
authors.lastname: Iorio
mesh_terms: D055162:Biocatalysis; D019149:Bioreactors; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005632:Fructose / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry*; D008863:Microspheres
chemical_list: D004800:Enzymes, Immobilized; D005632:Fructose; D006026:Glycoside Hydrolases; C023086:inulinase