Activation of rat brain adenylate cyclase by proteases: involvement of distinct protein components in the activation by ?-chymotrypsin and trypsin.
Adenylate cyclase in synaptic plasma membranes from rat brain is activated by ?-chymotrypsin or trypsin. These proteases also activate adenylate cyclase reconstituted from the catalytic subunit of adenylate cyclase and the partially purified fraction of the GTP-binding proteins containing both the stimulatory and inhibitory GTP-binding proteins. Properties of the activation of reconstituted adenylate cyclase by the proteases are as follows. (1) The proteases do not directly activate the catalytic subunit. However, the pre-treatment of the partially purified GTP-binding proteins with ?-chymotrypsin (100 ?g/ml) increases the subsequently reconstituted cyclase activity at least 3-fold. Trypsin (10-30 ?g/ml) much more weakly enhances the cyclase activity. (2) ?-Chymotrypsin and trypsin synergistically activate the cyclase. (3) Trypsin but not ?-chymotrypsin no longer activates the cyclase when the purified stimulatory GTP-binding protein (Gs) replaces the partially purified GTP-binding proteins. (4) The stimulatory effects of ?-chymotrypsin and trypsin on the cyclase activity are little or slight unless 5?-guanylylimidodiphosphate (Gpp(NH)p) is present in the reconstitution. (5) The purified ??-subunits of the GTP-binding proteins markedly inhibit adenylate cyclase. This inhibition is nearly completely attenuated by treating the ??-subunits with ?-chymotrypsin (> 10 ?g/ml). (6) Trypsin (1-10 ?g/ml) inactivates the GTPase of the ?-subunit of the inhibitory GTP-binding protein (Gi). This inactivation of the GTPase seems to correlate with the activation of the reconstituted adenylate cyclase by trypsin. We conclude that two distinct protein components are involved in the activation of adenylate cyclase by ?-chymotrypsin and trypsin. One component sensitive to ?-chymotrypsin is probably the ??-subunits of the GTP-binding proteins. The other component sensitive to trypsin may be the ?-subunit of Gi.
Emmom
K
K
Department of Pharmacology, Saga Medical School, Saga 840-01, Japan.
Asakawa
T
T