[Expression of NF-kappaB and its downstream products in human umbilical vascular endothelial cells treated with paraquat].
OBJECTIVE
To investigate the expression of NF-kappaB and its downstream products in the human umbilical vascular endothelial cells (HUVEC) treated with paraquat.


METHODS
The HUVEC were cultured in vitro. The activation and location of NF-kappaB P65 protein were detected by immunocytochemical method at 8 h, 24 h and 48 h after treatment with paraquat. The mRNA expression of IL-6 and IL-8 were detected by RT-PCR.


RESULTS
Immunocytochemical analyses revealed that the activity subunit of NF-kappaB P65 was located in cytoplasm of normal HUVEC. When the cells were treated with paraquat, positive staining in nuclear was observed. The sum of positive nuclear got more as the paraquat treated time increased. The RT-PCR verified the mRNA expression of IL-6 and IL-8 was up-regulated until 48 h after treatment with paraquat.


CONCLUSIONS
NF-kappaB could be activated at the early phase of paraquat poisoning. The activated NF-kappaB will induce the synthesis of inflammatory factors such as IL-6, IL-8 and then induce the inflammatory reaction. The inflammatory reaction have participated the tissue damage at the early phase of paraquat poisoning.
Yu
Hai-fang
HF
Department of Emergency, West China Hospital, Sichuan University, Chengdu 610041, China.
Nie
Hu
H
D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D006540:Herbicides / Q000633:toxicity; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D010269:Paraquat / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology; D015854:Up-Regulation / Q000187:drug effects
C508606:CXCL8 protein, human; D006540:Herbicides; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D016328:NF-kappa B; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D010269:Paraquat