Measurement of sVEGF R1 and PlGF in serum: comparing prototype assays from Beckman Coulter, Inc. to R&D Systems microplate assays.
OBJECTIVE
To compare the performance of prototype Access® sVEGF R1 and PlGF automated immunoassays from Beckman Coulter to the Quantikine® microplate ELISA assays by R&D Systems.


METHODS
Samples obtained from pregnant women, non-pregnant women and men were assayed according to manufacturers' instructions.


RESULTS
Compared to the Quantikine assays, the Access assays demonstrated improved precision, increased sensitivity, broader dynamic ranges, and reduced analysis time. The Access assays were found to be specific for free sVEGF R1 and free PlGF.


CONCLUSIONS
There was good correlation between the Access and Quantikine assays. Superior performance by Access assays may have important prenatal diagnostic implications.
Wothe
Donald
D
Abbott Northwestern Hospital, Minneapolis, MN, USA.
Gaziano
Emanuel
E
Sunderji
Shiraz
S
Romero
Roberto
R
Kusanovic
Juan Pedro
JP
Rogers
Linda
L
Hodges-Savola
Cheryl
C
Roberts
Sean
S
Wassenberg
James
J
D000328:Adult; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D000072483:Placenta Growth Factor; D011225:Pre-Eclampsia / Q000097:blood / Q000175:diagnosis; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood*; D012680:Sensitivity and Specificity; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood*
C000608036:PGF protein, human; D011257:Pregnancy Proteins; D000072483:Placenta Growth Factor; D040281:Vascular Endothelial Growth Factor Receptor-1