A snake venom peptide with the contrary effects on rat stomach fundus and guinea pig ileum.
A bradykinin potentiating peptide (BPP), Thr-Pro-Pro-Ala-Gly-Pro-Asp-Val-Gly-Pro-Arg-OH, was isolated from the venoms of Crotalus viridis viridis (here named Cvv peptide). Compared with other BPP, Cvv peptide has special Thr at N-terminal and Arg at C-terminal. In order to clarify whether these two special amino acids lead to special bioactivities relative to other BPPs, we made bioassays on isolated guinea pig ileum (GPI) and rat stomach fundus. Cvv peptide can observably inhibit bradykinin's contractivity on GPI, but potentiate the bradykinin-induced contractivity on rat stomach fundus. The discrepant bioactivity of Cvv peptide may occur via binding different receptors, B2 receptor on GPI and anaphylatoxin C3a receptor on rat stomach fundus, respectively.
Wang
Sha-Sha
SS
Institute of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, Lanzhou, China.
Zeng
Yu-Ling
YL
Zhang
Wen-Wei
WW
Dong
Shou-Liang
SL
D000818:Animals; D003435:Crotalid Venoms / Q000302:isolation & purification / Q000494:pharmacology*; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects*; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D010455:Peptides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D043782:Receptor, Bradykinin B2 / Q000187:drug effects; D043562:Receptors, G-Protein-Coupled / Q000187:drug effects; D013270:Stomach / Q000187:drug effects*
D003435:Crotalid Venoms; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D043782:Receptor, Bradykinin B2; D043562:Receptors, G-Protein-Coupled; C532341:anaphylatoxin receptor C3a, rat; C073977:peptide 74