Ultrastructural reconstruction of Taenia ovis oncospheres from serial sections.
The cellular organisation of Taenia ovis oncospheres is interpreted from ultrathin serial sections and transmission electron microscopy following high pressure freezing and freeze-substitution. The surface of a hatched, non-activated T. ovis oncosphere is covered by an oncospheral membrane below which is the tegument bearing microvilli. The basal lamina of the tegument is underlain by broad bands of peripheral somatic musculature. Three pairs of hooks and associated muscles are present in the somatophoric third of the oncosphere. Approximately 19 cells of seven different types were identified which include: (i) a quadri-nucleated syncytium of penetration gland type 1 containing two lateral pairs of cell bodies interconnected by narrow cytoplasmic bridges (PG1); (ii) a quadri-nucleated syncytium of penetration gland type 2 (PG2); (iii) a single-nucleated median mesophoric gland cell; (iv) 10 somatic cells; (v) two germinative cells; (vi) two nerve cells; and (vii) a pair of median somatophoric cells. This study provides a clear understanding of the morphology of T. ovis oncospheres and forms the basis for further investigations into the biology of taeniid oncospheres.
Jabbar
Abdul
A
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia. jabbara@unimelb.edu.au
Crawford
Simon
S
Młocicki
Daniel
D
Swiderski
Zdzisław P
ZP
Conn
David B
DB
Jones
Malcolm K
MK
Beveridge
Ian
I
Lightowlers
Marshall W
MW
D000818:Animals; D004285:Dogs; D046529:Microscopy, Electron, Transmission; D012756:Sheep; D013621:Taenia / Q000033:anatomy & histology / Q000648:ultrastructure*; D013622:Taeniasis / Q000469:parasitology