title: Chemical models and their mechanistic implications for the transformation of 6-cyanouridine 5'-monophosphate catalyzed by orotidine 5'-monophosphate decarboxylase.
abstract: The reactions of 6-cyano-1,3-dimethyluracil have been studied as chemical models to illustrate the mechanism for the transformation of 6-cyanouridine 5'-monophosphate (6-CN-UMP) to barbiturate ribonucleoside 5'-monophosphate (BMP) catalyzed by orotidine 5'-monophosphate decarboxylase (ODCase). The results suggest that the Asp residue in the ODCase active site plays the role of a general base in the transformation.
authors.affiliation: Department of Chemistry, National Taiwan Normal University, No.88, Sec.4, Ting-Zhou Road, Taipei, 11677, Taiwan.
authors.forename: Yuen-Jen
authors.initials: YJ
authors.lastname: Wu
authors.forename: Chen-Chieh
authors.initials: CC
authors.lastname: Liao
authors.forename: Cheng-Hung
authors.initials: CH
authors.lastname: Jen
authors.forename: Yu-Chiao
authors.initials: YC
authors.lastname: Shih
authors.forename: Tun-Cheng
authors.initials: TC
authors.lastname: Chien
mesh_terms: D001463:Barbiturates / Q000737:chemistry; D055162:Biocatalysis; D020134:Catalytic Domain; D008956:Models, Chemical; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000737:chemistry / Q000378:metabolism*; D014542:Uridine Monophosphate / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism
chemical_list: C513367:6-cyanouridine 5'-O-monophosphate; C541623:6-hydroxyuridine 5'-monophosphate; D001463:Barbiturates; D014542:Uridine Monophosphate; D009964:Orotidine-5'-Phosphate Decarboxylase