Ethyl pyruvate downregulates tumor necrosis factor alpha and interleukin (IL)-6 and upregulates IL-10 in lipopolysaccharide-stimulated canine peripheral blood mononuclear cells.
We investigated the anti-inflammatory effects of ethyl pyruvate (EP) on LPS-stimulated canine PBMCs in vitro. We found that EP treatment inhibited the mRNA expressions of proinflammatory cytokines (TNF-α and IL-6), but induced mRNA expression of anti-inflammatory cytokines (IL-10). ELISA measurements revealed that EP also effectively downregulates the LPS-induced increase in proinflammatory cytokine release, while upregulating anti-inflammatory cytokine release. These data indicate that EP could be an effective anti-inflammatory agent in dogs.
Yu
Do-Hyeon
DH
College of Veterinary Medicine & Bio-Safety Research Institute, Chonbuk National University, Jeonju, Jeonbuk, South Korea.
Noh
Dong-Ho
DH
Song
Ru-Hui
RH
Park
Jinho
J
D000818:Animals; D004285:Dogs; D015536:Down-Regulation; D005421:Flavoring Agents / Q000494:pharmacology; D016753:Interleukin-10 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D011773:Pyruvates / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000235:genetics*; D015854:Up-Regulation
D005421:Flavoring Agents; D015850:Interleukin-6; D008070:Lipopolysaccharides; D011773:Pyruvates; D014409:Tumor Necrosis Factor-alpha; C046522:ethyl pyruvate; D016753:Interleukin-10