Reduction of circulating endothelial cells in peripheral blood of ALS patients.
BACKGROUND
Amyotrophic Lateral Sclerosis (ALS) treatment is complicated by the various mechanisms underlying motor neuron degeneration. Recent studies showed that the blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) are compromised in an animal model of ALS due to endothelial cell degeneration. A later study demonstrated a loss of endothelium integrity in the spinal cords of ALS patients. Since circulating endothelial cells (CECs) in the peripheral blood are associated with endothelium damage, being detached dysfunctional endothelial cells, we hypothesized that CEC levels may reflect endothelium condition in ALS patients.


RESULTS
CEC levels were estimated in whole blood smears from ALS patients with moderate stage ((M)ALS), severe stage ((S)ALS), and healthy controls by CD146 expression using immunocytochemistry. A significant reduction of CECs was detected in (M)ALS and (S)ALS patients.


CONCLUSIONS
CECs did not predict endothelium state in ALS patients; however, endothelial damage and/or impaired endothelium repair may occur in ALS leading to BBB/BSCB dysfunction. Reduced CECs in peripheral blood of ALS patients may indicate different mechanisms of endothelial damage and repair, rather than only detachment of dysfunctional endothelial cells. Although a potential mechanism of CEC reduction is discussed, establishing a reliable indicator of endothelial dysfunction/damage is important for evaluation of BBB/BSCB status in ALS patients during disease progression.
Garbuzova-Davis
Svitlana
S
Center of Excellence for Aging and Brain Repair, College of Medicine, University of South Florida, Tampa, Florida, United States of America. sgarbuzo@health.usf.edu
Woods
Robert L
RL
Louis
Michael K
MK
Zesiewicz
Theresa A
TA
Kuzmin-Nichols
Nicole
N
Sullivan
Kelly L
KL
Miller
Amber M
AM
Hernandez-Ontiveros
Diana G
DG
Sanberg
Paul R
PR
D000690:Amyotrophic Lateral Sclerosis / Q000097:blood* / Q000473:pathology*; D051929:CD146 Antigen / Q000378:metabolism; D016022:Case-Control Studies; D002465:Cell Movement; D003710:Demography; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged
D051929:CD146 Antigen; C497902:MCAM protein, human