Handheld mechanical cell lysis chip with ultra-sharp silicon nano-blade arrays for rapid intracellular protein extraction.
This paper presents a handheld mechanical cell lysis chip with ultra-sharp nano-blade arrays fabricated by simple and cost effective crystalline wet etching of (110) silicon. The ultra-sharp nano-blade array is simply formed by the undercutting of (110) silicon during the crystalline wet etching process. Cells can be easily disrupted by the silicon nano-blade array without the help of additional reagents or electrical sources. Based on the bench-top test of the proposed device, a handheld mechanical cell lysis chip with the nano-blade arrays is designed and fabricated for direct connection to a commercial syringe. The direct connection to a syringe provides rapid cell lysis, easy handling, and minimization of the lysate dead volume. The protein concentration in the cell lysate obtained by the proposed lysis chip is quantitatively comparable to the one prepared by a conventional chemical lysis method.
Yun
Sung-Sik
SS
Department of Nanobio Materials and Electronics, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju, Republic of Korea 500-712.
Yoon
Sang Youl
SY
Song
Min-Kyung
MK
Im
Sin-Hyeog
SH
Kim
Sohee
S
Lee
Jong-Hyun
JH
Yang
Sung
S
D002458:Cell Fractionation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007424:Intracellular Fluid / Q000737:chemistry; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins