title: Variation in Streptococcus pyogenes NAD+ glycohydrolase is associated with tissue tropism.
abstract: Streptococcus pyogenes is an important pathogen that causes a variety of diseases. The most common infections involve the throat (pharyngitis) or skin (impetigo); however, the factors that determine tissue tropism and severity are incompletely understood. The S. pyogenes NAD(+) glycohydrolase (SPN) is a virulence factor that has been implicated in contributing to the pathogenesis of severe infections. However, the role of SPN in determining the bacterium's tissue tropism has not been evaluated. In this report, we examine the sequences of spn and its endogenous inhibitor ifs from a worldwide collection of S. pyogenes strains. Analysis of average pairwise nucleotide diversity, average number of nucleotide differences, and ratio of nonsynonymous to synonymous substitutions revealed significant diversity in spn and ifs. Application of established models of molecular evolution shows that SPN is evolving under positive selection and diverging into NAD(+) glycohydrolase (NADase)-active and -inactive subtypes. Additionally, the NADase-inactive SPN subtypes maintain the characteristics of a functional gene while ifs becomes a pseudogene. Thus, NADase-inactive SPN continues to evolve under functional constraint. Furthermore, NADase activity did not correlate with invasive disease in our collection but was associated with tissue tropism. The ability to cause infection at both the pharynx and the skin ("generalist" strains) is correlated with NADase-active SPN, while the preference for causing infection at either the throat or the skin ("specialist" strains) is associated with NADase-inactive SPN. These findings suggest that SPN has a NADase-independent function and prompt a reevaluation of the role of SPN in streptococcal pathogenesis.
authors.affiliation: Department of Internal Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Riddle
authors.forename: Debra E
authors.initials: DE
authors.lastname: Bessen
authors.forename: Michael G
authors.initials: MG
authors.lastname: Caparon
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics / Q000502:physiology; D014644:Genetic Variation; D006239:Haplotypes; D008969:Molecular Sequence Data; D009244:NAD+ Nucleosidase / Q000235:genetics / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000235:genetics; D018523:Tropism / Q000502:physiology*
chemical_list: D004269:DNA, Bacterial; D009244:NAD+ Nucleosidase