Biochemical characterization of various populations of isolated bovine adrenal chromaffin cells.
Various populations of bovine adrenal chromaffin cells were isolated first by successive digestions with collagenase (original cell preparation) followed by sedimentation through a stepwise bovine serum albumin gradient (cell layers I, II and III). At the fine structural level, the ratios between the number of adrenaline-cells and noradrenaline-cells were 1.9 in the original cell preparation and 0.9, 2.0 and 4.6 in cell layers I, II and III, respectively. The catecholamine content of each cell population was also measured by spectrofluorometry. The original cell preparation contained 20.1 and 12.2 nmol per 10(6) cells of adrenaline and noradrenaline, respectively. Each cell layer had similar total amount of catecholamines (from 38.3 to 40 nmol per 10(6) cells) but their adrenaline/noradrenaline content ratios varied from 0.6 in cell layer 1 to 1.3 and 3.3 in cell layers II and III, respectively. Incubation of the cells in the presence of acetylcholine (50 ?M) induced a release of catecholamines which was proportional to the cell content of each amine. However, the percentage of total cell content released was much higher in cell layer I (20%) than in cell layers II (8%) and III (5%). Finally, each cell population was also analyzed for its ability to respond to a muscarinic stimulation of cyclic GMP level and to bind [(3)H]etorphine, a highly potent opiate agonist. Acetylcholine induced 3.15-, 2.15- and 4.21-fold increases in the levels of cyclic GMP in the original cell preparation, cell layers II and III, respectively, but not in cell layer I. Conversely, the high affinity opiate binding site for [(3)H]etorphine was almost exclusively confined to cell layer III (B(max) of 28.4 fmol per 10(6) cells as compared with 2.8-7.5 fmol in the other cell preparations). These results indicate that bovine adrenal chromaffin cells can be separated according to their content in adrenaline and noradrenaline and their response to nicotinic, muscarinic and opiate stimuli.
Lemaire
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S
Department of Physiology and Pharmacology, Centre Hospitalier Universitaire, Sherbrooke, Québec, Canada J1H 5N4.
Dumont
M
M
Mercier
P
P
Lemaire
I
I
Calvert
R
R