title: [Construction of cDNA library of protozoan ciliates Euplotes octocarinatus].
abstract: The protein biosynthesis in cells is an open process cooperatively regulated by many protein factors and enzymes, which form a complicated protein network and signal transduction pathway for mRNA metabolism and protein translation.
  
  
  OBJECTIVE
  To provide a platform for studying on the function of proteins involving in process of translation termination and the relationship among these proteins in ciliates.
  
  
  METHODS
  We constructed the cDNA library of protozoan ciliates Euplotes octocarinatus strictly following the procedure of BD Matchmaker Library Construction & Screening kit from Clontech.
  
  
  RESULTS
  We obtained a cDNA library of ciliates Euplotes, the titer of which was about 2.437 x 10(7) cfu/mL, which could suffice for functional gene screen. By using the class II polypeptide release factor (eRF3) as bait protein, we obtained some putative genes, including a partial cDNA putatively encoding RNA helicase.
  
  
  CONCLUSIONS
  This library will provide a convenient platform for identifying the functional genes in ciliates.
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of The Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China. bfchai@sxu.edu.cn
authors.forename: Baofeng
authors.initials: B
authors.lastname: Chai
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Hao
authors.forename: Cui
authors.initials: C
authors.lastname: Li
authors.forename: Quan
authors.initials: Q
authors.lastname: Shen
authors.forename: Aihua
authors.initials: A
authors.lastname: Liang
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics; D016812:Euplotes / Q000235:genetics*; D015723:Gene Library; D010454:Peptide Termination Factors / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics
chemical_list: D016054:DNA, Protozoan; D010454:Peptide Termination Factors; D015800:Protozoan Proteins; C087971:peptide-chain-release factor 3