[Study on the inhibitory effect of ALA-PDT on the proliferation of fibroblasts from keloid].
OBJECTIVE
To investigate the effects of ALA-PDT on the proliferation of fibroblasts from keloid in vitro, and to find the most efficient concentration of photosensitizer and the energy of He-Ne laser light.


METHODS
The fibroblast cells from keloid cultured in vitro were divided into blank control group, laser light group (only with different dose energy of He-Ne laser light), photosensitizer group(only with different concentration of ALA) and ALA-PDT group (treated by photosensitizer and laser light). After the above treatments were finished, the inhibition rate of each group was measured with MTT method.


RESULTS
There is no obvious inhibitory effect on the fibroblast cells from keloid in laser light group and photosensitizer group, In ALA-PDT groups, when the concentration of ALA and the dose of energy of He-Ne laser light reached some extent (>0.125 mmol/L and >10 J/cm2 respectively), the inhibitory effect of ALA-PDT kept rising with the increase of the concentration of ALA and the energy of He-Ne laser light. The most appropriate concentration for ALA is 0.25 mmol/L, while the energy for He-Ne laser light is 40 J/cm2.


CONCLUSIONS
The inhibitory effect, of ALA-PDT on the proliferation of fibroblasts from keloid could be enhanced with the increase of photosensitizer concentration and laser light energy.
Zhong
Jun-bo
JB
Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Cen
Ying
Y
Liu
Quan
Q
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D007627:Keloid / Q000473:pathology*; D007834:Lasers; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology
D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid