title: The complete genome sequence of Canna yellow streak virus.
abstract: Canna yellow streak virus (Potyvirus, Potyviridae) was sequenced using the novel method of next-generation pyrosequencing. The complete genome was found to be 9,502 nucleotides excluding the poly-A tail with a predicted genome organisation typical for a member of the genus Potyvirus. As with other potyviruses that infect monocotyledons, some of the predicted cleavage sites of the polyprotein genome were unusual, such as a glutamic acid/threonine (E/T) between the CI and 6K2 proteins and a glutamic acid/aspartic acid (E/D) between the NIa and NIb proteins. Evidence of the presence of endogenous pararetroviruses in the canna genome was found from the large number of sequences obtained with this method.
authors.affiliation: The Food and Environment Research Agency, Sand Hutton, York, UK. wendy.monger@fera.gsi.gov.uk
authors.forename: W A
authors.initials: WA
authors.lastname: Monger
authors.forename: I P
authors.initials: IP
authors.lastname: Adams
authors.forename: R H
authors.initials: RH
authors.lastname: Glover
authors.forename: B
authors.initials: B
authors.lastname: Barrett
mesh_terms: D001483:Base Sequence; D027581:Cannabaceae / Q000821:virology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification