title: Histomorphometrical analysis following augmentation of infected extraction sites exhibiting severe bone loss and primarily closed by intrasocket reactive soft tissue.
abstract: BACKGROUND
  Intrasocket reactive soft tissue can be used for primary closure during augmentation of infected extraction sites exhibiting severe bone loss prior to implant placement. The present study evaluated the histological characteristics of the initially used intrasocket reactive soft tissue, the overlying soft tissue, and the histomorphometry of the newly formed bone during implant placement.
  
  
  METHODS
  Thirty-six consecutive patients (43 sites) were included in the study. Extraction sites demonstrating extensive bone loss on preoperative periapical and panoramic radiographs served as inclusion criteria. Forty-three implants were inserted after a healing period of 6 months. Porous bovine xenograft bone mineral was used as a single bone substitute. The intrasocket reactive soft tissue was sutured over the grafting material to seal the coronal portion of the socket. Biopsies of the intrasocket reactive soft tissue at augmentation, healed mucosa, and bone cores at implant placement were retrieved and evaluated.
  
  
  RESULTS
  The intrasocket reactive soft tissue demonstrated features compatible with granulation tissue and long junctional epithelium. The mucosal samples at implant placement demonstrated histopathological characteristics of keratinized mucosa with no residual elements of granulation tissue. Histomorphometrically, the mean composition of the bone cores was - vital bone 40 ± 19% (13.7-74.8%); bone substitute 25.7 ± 13% (0.6-51%); connective tissue 34.3 ± 15% (13.8-71.9%).
  
  
  CONCLUSIONS
  Intrasocket reactive soft tissue used for primary closure following ridge augmentation is composed of granulation tissue and long junctional epithelium. At implant placement, clinical and histological results demonstrate its replacement by keratinized gingiva. The histomorphometrical results reveal considerable bone formation. Fresh extraction sites of hopeless teeth demonstrating chronic infection and severe bone loss may be grafted simultaneously with their removal.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tel Aviv University, Tel Aviv, Israel. ofer.mardinger@gmail.com
authors.forename: Ofer
authors.initials: O
authors.lastname: Mardinger
authors.forename: Marilena
authors.initials: M
authors.lastname: Vered
authors.forename: Gavriel
authors.initials: G
authors.lastname: Chaushu
authors.forename: Joseph
authors.initials: J
authors.lastname: Nissan
mesh_terms: D000328:Adult; D000368:Aged; D016301:Alveolar Bone Loss / Q000473:pathology / Q000601:surgery*; D000540:Alveolar Ridge Augmentation / Q000379:methods*; D019072:Antibiotic Prophylaxis; D001424:Bacterial Infections; D001861:Bone Regeneration; D018786:Bone Substitutes; D055113:Chronic Periodontitis / Q000601:surgery*; D015331:Cohort Studies; D003758:Dental Implantation, Endosseous; D004846:Epithelial Attachment / Q000473:pathology; D005260:Female; D005881:Gingiva / Q000502:physiology*; D006097:Granulation Tissue / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008903:Minerals; D009061:Mouth Mucosa / Q000473:pathology; D011446:Prospective Studies; D062326:Re-Epithelialization; D014081:Tooth Extraction; D020390:Tooth Socket / Q000473:pathology / Q000601:surgery*; D055815:Young Adult
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D008903:Minerals