The early administration of granulocyte colony-stimulating factor increases the engraftment of transplanted bone marrow-derived cells into the olfactory epithelium damaged by methimazole.
In 2005 we reported that transplanted bone marrow-derived cells were engrafted into the olfactory epithelium and then had characteristics of olfactory neuron cells. However, the engraftment rate was far from a practical level. Granulocyte colony-stimulating factor (G-CSF) is known to mobilize stem cells from the bone marrow into the circulation. To assess the efficacy of G-CSF on engrafting transplanted bone marrow-derived cells into the olfactory epithelium, we performed the time window study of G-CSF administration. After C57BL/6 mice transplanted with bone marrow cells from GFP mice received selective damage of the olfactory epithelium by methimazole, G-CSF was administrated into the recipient mice at different time windows. A statistical analysis demonstrated that the early administration of G-CFS was appropriate to increase the engraftment rate of bone marrow-derived cells into the olfactory epithelium. Cells with double-immunostaining for GFP and OMP, GAP43 or cytokeratin were found in the olfactory epithelium of recipient mice. These results suggest that bone marrow-derived cells are engrafted as stem cells of the olfactory tissue and that the early administration of G-CSF is more effective to increase the engraftment rate of bone marrow-derived cell into the olfactory epithelium damaged by methimazole.
Nishizaki
Kazunori
K
Department of Otolaryngology-Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan. nishizak@cc.okayama-u.ac.jp
Yoshinobu
Junko
J
Tsujigiwa
Hidetsugu
H
Orita
Yorihisa
Y
Yamada
Masao
M
D000818:Animals; D016026:Bone Marrow Transplantation; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000378:metabolism; D008713:Methimazole / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D051842:Olfactory Marker Protein / Q000378:metabolism; D009831:Olfactory Mucosa / Q000166:cytology* / Q000378:metabolism
D019922:GAP-43 Protein; D051842:Olfactory Marker Protein; C497598:Omp protein, mouse; D016179:Granulocyte Colony-Stimulating Factor; D008713:Methimazole; D007633:Keratins