A sensitive immunotransfer method for the detection of oligodendroglial plasma membrane antigens.
Efficient transfer of a wide molecular weight range of plasma membrane proteins from polyacrylamide gels to nitrocellulose paper has been achieved. Using published immunodetection procedures (sensitivity: 1 ng protein), two major antigens of molecular weights of 71,000 and 82,000 have been identified in oligodendroglial plasma membranes. This immunotransfer method will be extremely useful for the detection of low levels of antigen in complex membrane protein mixtures.
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The Johns Hopkins University, School of Medicine, Baltimore, Md, U.S.A.
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