title: Rapid identification of multidrug-resistant tuberculosis isolates in treatment failure or relapse patients in Bangui, Central African Republic.
abstract: Multidrug-resistant (MDR) strains were identified in 40% of 54 strains from patients presenting with tuberculosis (TB) treatment failure or relapse in Bangui, Central African Republic. Results obtained with the MTBDRplus line-probe assay or rpoB sequencing were 86% concordant with rifampicin (RMP) resistant phenotypes, while the amplification refractory mutation system test was 71% concordant. No mutation was found in RMP-susceptible strains. MTBDRplus and sequencing were concordant with the detection of the S315T mutation in katG in 95% of MDR strains. Sequencing of pncA suggested pyrazinamide resistance in 50% of MDR strains. Knowledge of these resistances should help to implement treatment in low-income countries.
authors.affiliation: Laboratoire des Mycobactéries, Institut Pasteur de Bangui, Bangui, Central African Republic.
authors.forename: F
authors.initials: F
authors.lastname: Minime-Lingoupou
authors.forename: C
authors.initials: C
authors.lastname: Pierre-Audigier
authors.forename: E
authors.initials: E
authors.lastname: Kassa-Kélémbho
authors.forename: N
authors.initials: N
authors.lastname: Barilone
authors.forename: G
authors.initials: G
authors.lastname: Zandanga
authors.forename: J
authors.initials: J
authors.lastname: Rauzier
authors.forename: V
authors.initials: V
authors.lastname: Cadet-Daniel
authors.forename: A
authors.initials: A
authors.lastname: Le Faou
authors.forename: B
authors.initials: B
authors.lastname: Gicquel
mesh_terms: D000995:Antitubercular Agents / Q000627:therapeutic use*; D002488:Central African Republic / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D015994:Incidence; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012008:Recurrence; D012189:Retrospective Studies; D017211:Treatment Failure; D018088:Tuberculosis, Multidrug-Resistant / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial