The transdehydrogenase genes KlNDE1 and KlNDI1 regulate the expression of KlGUT2 in the yeast Kluyveromyces lactis.
KlNDE1 and KlNDI1 code for two inner mitochondrial membrane transdehydrogenases involved in the maintenance of the intracellular NAD(P)H redox balance. The function of these genes during the utilization of fermentative and respiratory carbon sources was studied. During growth in glucose, deletion of KlNDE1 and KlNDI1 led to an altered kinetic of ethanol and glycerol accumulation compared with the wild type; in addition, KlndiDelta was unable to grow in respiratory substrates. Northern analysis and GFP-fusion experiments showed that KlNDE1 and KlNDI1 regulate the expression of KlGUT2, a component of the glycerol-3-phosphate shuttle. Moreover, both genes seem to be involved in the biogenesis of the mitochondrial tubular network.
Saliola
Michele
M
Department of Cell and Developmental Biology, University of Rome La Sapienza, Rome, Italy. michele.saliola@uniroma1.it
D'Amici
Sirio
S
Sponziello
Marialuisa
M
Mancini
Patrizia
P
Tassone
Paola
P
Falcone
Claudio
C
D000431:Ethanol / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis*; D007716:Kluyveromyces / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*
D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D000431:Ethanol; D010088:Oxidoreductases; D005993:Glycerolphosphate Dehydrogenase; D005990:Glycerol