Long-term evaluation of myoblast seeded patches implanted on infarcted rat hearts.
Cell transplantation presents great potential for treatment of patients with severe heart failure. However, its clinical application was revealed to be more challenging than initially expected in experimental studies. Further investigations need to be undertaken to define the optimal treatment conditions. We previously reported on the epicardial implantation of a bio-engineered construct of skeletal myoblast-seeded polyurethane and its preventive effect on progression toward heart failure. In the present study, we present a long-term evaluation of this functional outcome. Left anterior descending coronary ligation was performed in female Lewis rats. Two weeks later, animals were treated with either epicardial implantation of biograft, acellular scaffold, sham operation, or direct intramyocardial skeletal myoblast injection. Functional assessments were performed with serial echocardiographies every 3 months and end point left ventricle pressure was assessed. Hearts were then harvested for histological examinations. Myocardial infarction induced a slow and progressive reduction in fractional shortening after 3 months. Progression toward heart failure was significantly prevented for up to 6 months after injection of myoblasts and for up to 9 months following biograft implantation. Nevertheless, this effect vanished after 12 months, with immunohistological examinations revealing an absence of the transplanted myoblasts within the scaffold. We demonstrated that tissue therapy is superior to cell therapy for stabilization of heart function. However, beneficial effects are transient.
Giraud
Marie-Noëlle
MN
Department of Cardiovascular Surgery, Inselspital, Bern University Hospital, Switzerland. marienoelle.giraud-flueck@insel.ch
Flueckiger
Remy
R
Cook
Stéphane
S
Ayuni
Erick
E
Siepe
Matthias
M
Carrel
Thierry
T
Tevaearai
Hendrik
H
D000818:Animals; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D004452:Echocardiography; D005260:Female; D006439:Hemodynamics; D032448:Myoblasts, Skeletal / Q000637:transplantation*; D009203:Myocardial Infarction / Q000628:therapy*; D009206:Myocardium / Q000473:pathology; D011140:Polyurethanes / Q000737:chemistry; D051381:Rats; D011917:Rats, Inbred Lew; D054457:Tissue Scaffolds / Q000737:chemistry*; D016277:Ventricular Function, Left
D011140:Polyurethanes