Synaptic connectivity of serotonergic axons in the olfactory glomeruli of the rat olfactory bulb.
Although the major mode of transmission for serotonin in the brain is volume transmission, previous anatomical studies have demonstrated that serotonergic axons do form synaptic contacts. The olfactory glomeruli of the olfactory bulb of mammals receive a strong serotonergic innervation from the dorsal and medial raphe nuclei. In the present report, we investigate the synaptic connectivity of these serotonergic axons in the glomerular neuropil of the rat olfactory bulb. Our study shows that serotonergic axons form asymmetrical synaptic contacts on dendrites within the glomerular neuropil. Analyzing the neurochemical nature of the synaptic targets, we have found that 55% of the synapses were on GABA-immunopositive profiles and 45% on GABA-immunonegative profiles. These data indicate that barely half of the contacts were found in GABA-immunonegative profiles and half of the synapses in GABA-positive dendrites belonging to type 1 periglomerular cells. Synaptic contacts from serotonergic axons on dendrites of principal cells cannot be excluded, since some of the GABA-immunonegative postsynaptic profiles contacted by serotonergic axons had the typical ultrastructural features of bulbar principal cell dendrites. Altogether, our results suggest a complex action of the serotonergic system in the modulation of the bulbar circuitry.
Gracia-Llanes
F J
FJ
Departamento de Biología Celular, Facultad de Ciencias Biológicas, Universidad de Valencia, Burjasot, Valencia, Spain.
Blasco-Ibáñez
J M
JM
Nácher
J
J
Varea
E
E
Liberia
T
T
Martínez
P
P
Martínez-Guijarro
F J
FJ
Crespo
C
C
D000818:Animals; D001369:Axons / Q000502:physiology*; D007150:Immunohistochemistry; D007395:Interneurons / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D019581:Neuropil / Q000502:physiology* / Q000648:ultrastructure; D009830:Olfactory Bulb / Q000502:physiology* / Q000648:ultrastructure; D009832:Olfactory Nerve / Q000502:physiology / Q000648:ultrastructure; D017729:Presynaptic Terminals / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism*; D013569:Synapses / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D012701:Serotonin; D005680:gamma-Aminobutyric Acid