Effect of temperature during assembly on the structure and mechanical properties of peptide-based materials.
Mutually complementary, self-repulsive oligopeptide pairs were designed to coassemble into viscoelastic hydrogels. Peptide engineering was combined with biophysical techniques to investigate the effects of temperature on the structural and mechanical properties of the resulting hydrogels. Biophysical characterizations, including dynamic rheometry, small-angle X-ray scattering (SAXS), and fluorescence spectroscopy, were used to investigate hydrogelation at the bulk, fiber, and molecular levels, respectively. It has been found that temperature has a significant effect on the structure and mechanical properties of peptide-based biomaterials. Oligopeptide fibers assembled at 25 degrees C are formed faster and are two times thicker, and the resulting material is mechanically seven times stronger than that assembled at 5 degrees C.
Ramachandran
Sivakumar
S
Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Mohali-160062, India.
Taraban
Marc B
MB
Trewhella
Jill
J
Gryczynski
Ignacy
I
Gryczynski
Zygmunt
Z
Yu
Yihua Bruce
YB
D000595:Amino Acid Sequence; D055119:Elastic Modulus; D020100:Hydrogels / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry*; D012212:Rheology; D053838:Scattering, Small Angle; D013050:Spectrometry, Fluorescence; D013696:Temperature; D055682:Viscoelastic Substances / Q000737:chemistry*; D014961:X-Ray Diffraction
D020100:Hydrogels; D009842:Oligopeptides; D055682:Viscoelastic Substances