Glyceraldehyde-3-phosphate dehydrogenase interacts with phosphorylated Akt resulting from increased blood glucose in rat cardiac muscle.
Here we describe the interaction of phosphorylated approximately 40 kDa protein with phosphorylated Akt which is a serine/threonine kinase resulting from increased blood glucose in rat cardiac muscle. Mass spectrometry analysis revealed that this protein was glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Furthermore, increase in Akt and GAPDH phosporylation and induction of their association were both observed after insulin stimulation in the H9c2 cell line derived from embryonic rat ventricle. Moreover, the activation of GAPDH was upregulated when the GAPDH phosphorylation was increased. Our data suggest that GAPDH phosphorylation and association with Akt by insulin treatment have some bearing on the enhancement of GAPDH activity.
Baba
Takeshi
T
Department of Chemistry, Faculty of Medicine, Juntendo University, Inzai-shi, Chiba, Japan. t_baba@sakura.juntendo.ac.jp
Kobayashi
Hiroyuki
H
Kawasaki
Hiroaki
H
Mineki
Reiko
R
Naito
Hisashi
H
Ohmori
Daijiro
D
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002460:Cell Line; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D015151:Immunoblotting; D047468:Immunoprecipitation; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D007328:Insulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D051057:Proto-Oncogene Proteins c-akt