[Effect of imatinib at different concentrations on rat C6 glioma cell apoptosis and cell cycle].
OBJECTIVE
To investigate the effect of imatinib on rat C6 glioma cell apoptosis and cell cycle.


METHODS
MTT assay was used to determine the OD value of C6 glioma cells following treatment with imatinib at different concentrations (0.156, 10 and 15 micromo/L) for 24, 48 and 72 h. The cell apoptosis was assayed by Hochest/PI staining and the cell cycle changes were analyzed by flow cytometry.


RESULTS
Imatinib treatment resulted in increased number of apoptotic cells in a time- and dose-dependent manner. A 72-h treatment of the cells with imatinib at 10 and 15 micromo/L caused increased cell percentage in G(0)/G(1) phase to (68.53-/+0.83)% and (70.41-/+0.62)%, (P<0.01), decreased the percentage of G(2) phase cells to (14.48-/+0.12)% and (13.84-/+2.86)% (P<0.01), and decreased the percentage of S phase cells to (16.98-/+0.72)% and (15.78-/+2.28)%, respectively (P<0.01).


CONCLUSIONS
Imatinib can induce apoptosis and affect the distribution of the cell cycle of C6 cells in vitro.
Yang
Ling
L
Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Xu
Zhong-yuan
ZY
Chen
Xiao-hua
XH
Wang
Ke-wan
KW
Li
Guo-feng
GF
Chen
Zhi-liang
ZL
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001549:Benzamides; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D005910:Glioma / Q000473:pathology*; D000068877:Imatinib Mesylate; D010879:Piperazines / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D051381:Rats
D000970:Antineoplastic Agents; D001549:Benzamides; D010879:Piperazines; D011743:Pyrimidines; D000068877:Imatinib Mesylate