title: Bioinformatic analysis of Entamoeba histolytica SINE1 elements.
abstract: BACKGROUND
  Invasive amoebiasis, caused by infection with the human parasite Entamoeba histolytica remains a major cause of morbidity and mortality in some less-developed countries. Genetically E. histolytica exhibits a number of unusual features including having approximately 20% of its genome comprised of repetitive elements. These include a number of families of SINEs - non-autonomous elements which can, however, move with the help of partner LINEs. In many eukaryotes SINE mobility has had a profound effect on gene expression; in this study we concentrated on one such element - EhSINE1, looking in particular for evidence of recent transposition.
  
  
  RESULTS
  EhSINE1s were detected in the newly reassembled E. histolytica genome by searching with a Hidden Markov Model developed to encapsulate the key features of this element; 393 were detected. Examination of their sequences revealed that some had an internal structure showing one to four 26-27 nt repeats. Members of the different classes differ in a number of ways and in particular those with two internal repeats show the properties expected of fairly recently transposed SINEs - they are the most homogeneous in length and sequence, they have the longest (i.e. the least decayed) target site duplications and are the most likely to show evidence (in a cDNA library) of active transcription. Furthermore we were able to identify 15 EhSINE1s (6 pairs and one triplet) which appeared to be identical or very nearly so but inserted into different sites in the genome; these provide good evidence that if mobility has now ceased it has only done so very recently.
  
  
  CONCLUSIONS
  Of the many families of repetitive elements present in the genome of E. histolytica we have examined in detail just one - EhSINE1. We have shown that there is evidence for waves of transposition at different points in the past and no evidence that mobility has entirely ceased. There are many aspects of the biology of this parasite which are not understood, in particular why it is pathogenic while the closely related species E. dispar is not, the great genetic diversity found amongst patient isolates and the fact, which may be related, that only a small proportion of those infected develop clinical invasive amoebiasis. Mobile genetic elements, with their ability to alter gene expression may well be important in unravelling these puzzles.
authors.affiliation: Department of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK.
authors.forename: Derek M
authors.initials: DM
authors.lastname: Huntley
authors.forename: Ioannis
authors.initials: I
authors.lastname: Pandis
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Butcher
authors.forename: John P
authors.initials: JP
authors.lastname: Ackers
mesh_terms: D001483:Base Sequence; D019295:Computational Biology; D004748:Entamoeba histolytica / Q000235:genetics*; D020440:Gene Duplication; D018503:Genome, Protozoan / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger