Transient demyelination increases the efficiency of retrograde AAV transduction.
Adeno-associated virus (AAV) is capable of mediating retrograde viral transduction of central and peripheral neurons. This occurs at a relatively low efficiency, which we previously found to be dependent upon capsid serotype. We sought to augment retrograde transduction by providing increased axonal access to peripherally delivered AAV. Others have described utilizing full transection of peripheral nerves to mediate retrograde viral transduction of motor neurons. Here, we examined the ability of a transient demyelinating event to modulate levels of retrograde AAV transduction. Transient demyelination does not cause lasting functional deficits. Ethidium bromide (EtBr)-induced transient demyelination of the sciatic nerve resulted in significant elevation of retrograde transduction of both motor and sensory neurons. Retrograde transduction levels of motor neurons and heavily myelinated, large-diameter sensory neurons increased at least sixfold following peripheral delivery of self-complementary AAV serotype 1 (scAAV1) and serotype 2 (scAAV2), when preceded by demyelination. These findings identify a means of significantly enhancing retrograde vector transport for use in experimental paradigms requiring either retrograde neuronal identification and gene expression, or translational treatment paradigms.
Hollis
Edmund R
ER
Department of Neurosciences, University of California-San Diego, La Jolla, CA 92093, USA.
Jamshidi
Pouya
P
Lorenzana
Ariana O
AO
Lee
Jae K
JK
Gray
Steven J
SJ
Samulski
Richard J
RJ
Zheng
Binhai
B
Tuszynski
Mark H
MH
D000818:Animals; D003711:Demyelinating Diseases / Q000139:chemically induced* / Q000378:metabolism*; D000229:Dependovirus / Q000235:genetics*; D004996:Ethidium / Q000633:toxicity; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009046:Motor Neurons / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D012584:Sciatic Nerve / Q000187:drug effects* / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000378:metabolism; D014161:Transduction, Genetic / Q000379:methods*
D004996:Ethidium