Early treatment of transient focal cerebral ischemia with bovine PEGylated carboxy hemoglobin transfusion.
The effect of transfusion of PEGylated hemoglobin (PEG-Hb) was evaluated in anesthetized rats subjected to 2 hours of focal cerebral ischemia and 1 day of reperfusion. PEG-Hb was stored in the carboxy state (PEG-COHb) to reduce autooxidation and increase the shelf life. Transfusion of 10 ml/kg of PEG-COHb at 20 minutes of ischemia did not alter arterial blood pressure or increase red cell flux in the ischemic core. Plasma hemoglobin increased to only 0.6 g/dL, yet infarct volume was markedly decreased and neurological deficits were improved. We conclude that early topload transfusion of PEG-COHb protects the brain from ischemic stroke.
Klaus
Judith A
JA
Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University/JHMI, 600 North Wolfe Street, Baltimore, MD 21287, USA.
Kibler
Kathleen K
KK
Abuchowski
Abraham
A
Koehler
Raymond C
RC
D000818:Animals; D001784:Blood Gas Analysis; D001794:Blood Pressure; D001803:Blood Transfusion / Q000379:methods*; D001831:Body Temperature; D002263:Carboxyhemoglobin / Q000737:chemistry* / Q000627:therapeutic use*; D002417:Cattle; D004573:Electrolytes / Q000097:blood; D002546:Ischemic Attack, Transient / Q000097:blood / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D004573:Electrolytes; D011092:Polyethylene Glycols; D002263:Carboxyhemoglobin