[Influences of Panax notoginsenosid on spontaneous contraction of small intestine smooth muscle of rabbits in vitro].
OBJECTIVE
To observe the influences of Panax notoginsenosid(a compound of Chinese Traditional Medicine) on the spontaneous contraction of small intestine smooth muscle of rabbits in vitro and explore the mechanism.


METHODS
The influences of Panax notoginsenosid on the spontaneous contraction of small intestine in intacted rabbits(male or female) after the isothermal perfuse of small intestine in vitro were observed. Bay K8644 and nitro-L-arginine methylester (L-NAME) were added to the normal Tyrode's solution respectively before Panax notoginsenosid. In the Ca2+ free Tyrode's solution, rynodine was added before Panax notoginsenosid. The mechanism of Panax notoginsenosid was studied.


RESULTS
Panax notoginsenosid reduced the amplitude of contraction of small intestine smooth muscle in rabbits in a does-depended manner. Bay K8644 and L-NAME could completely block the inhibition of Panax notoginsenosid on the contraction of small intestine smooth muscle. Panax notoginsenosid inhibited significantly the intracellular calcium-depended contraction induced by rynodine in the Ca2+ free Tyrode's solution.


CONCLUSIONS
Panax notoginsenosid inhibits significantly the contraction of small intestine smooth muscle of rabbits in vitro. The mechanism may be related to increase NO concentration in small intestine smooth muscle so that inhibit extracellular Ca2+ inflowing via cell membrane and intracellular Ca2+ releasing via sarcoplasmic reticulum.
Li
Qian
Q
Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China. liqian963@163.com
Guan
Yue
Y
Ma
Hui-jie
HJ
Guo
Zan
Z
He
Rui-rong
RR
Zhang
Yi
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism; D003864:Depression, Chemical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000502:physiology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000378:metabolism / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism; D053723:Panax notoginseng / Q000737:chemistry*; D011817:Rabbits
D004365:Drugs, Chinese Herbal; D009569:Nitric Oxide; D002118:Calcium