One naive T cell, multiple fates in CD8+ T cell differentiation.
The mechanism by which the immune system produces effector and memory T cells is largely unclear. To allow a large-scale assessment of the development of single naive T cells into different subsets, we have developed a technology that introduces unique genetic tags (barcodes) into naive T cells. By comparing the barcodes present in antigen-specific effector and memory T cell populations in systemic and local infection models, at different anatomical sites, and for TCR-pMHC interactions of different avidities, we demonstrate that under all conditions tested, individual naive T cells yield both effector and memory CD8+ T cell progeny. This indicates that effector and memory fate decisions are not determined by the nature of the priming antigen-presenting cell or the time of T cell priming. Instead, for both low and high avidity T cells, individual naive T cells have multiple fates and can differentiate into effector and memory T cell subsets.
Gerlach
Carmen
C
Division of Immunology, Central Microarray Facility, and Bioinformatics and Statistics Group, The Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
van Heijst
Jeroen W J
JW
Swart
Erwin
E
Sie
Daoud
D
Armstrong
Nicola
N
Kerkhoven
Ron M
RM
Zehn
Dietmar
D
Bevan
Michael J
MJ
Schepers
Koen
K
Schumacher
Ton N M
TN
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D002454:Cell Differentiation / Q000276:immunology*; D019070:Cell Lineage / Q000276:immunology*; D007156:Immunologic Memory / Q000276:immunology; D008088:Listeriosis / Q000150:complications / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000150:complications / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology
D011948:Receptors, Antigen, T-Cell