Amphioxus SARM involved in neural development may function as a suppressor of TLR signaling.
Among five Toll/IL-1R resistance adaptors, sterile alpha and Toll/IL-1R resistance motif containing protein (SARM) is the only one conserved from Caenorhabditis elegans to human. However, its physiologic roles are hardly understood, and its involvement in TLR signaling remains debatable. In this study, we first demonstrated a predominant expression of amphioxus SARM (Branchiostoma belcheri tsingtauense SARM) in neural cells during embryogenesis and its predominant expression in the digestive system from larva to adult, suggesting its primitive role in neural development and a potential physiologic role in immunity. We further found that B. belcheri tsingtauense SARM was localized in mitochondria and could attenuate the TLR signaling via interacting with amphioxus MyD88 and tumor necrosis receptor associated factor 6. Thus, amphioxus SARM appears unique in that it may play dual functions in neural development and innate immunity by targeting amphioxus TLR signaling.
Yuan
Shaochun
S
State Key Laboratory of Biocontrol, National Engineering Research Center of South China Sea Marine Biotechnology, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, Guangzhou, China.
Wu
Kui
K
Yang
Manyi
M
Xu
Liqun
L
Huang
Ling
L
Liu
Huiling
H
Tao
Xin
X
Huang
Shengfeng
S
Xu
Anlong
A
D000595:Amino Acid Sequence; D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D043344:Chordata / Q000196:embryology / Q000235:genetics / Q000276:immunology*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D047468:Immunoprecipitation; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D055495:Neurogenesis / Q000276:immunology*; D043562:Receptors, G-Protein-Coupled; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000276:immunology*; D051193:Toll-Like Receptors / Q000276:immunology*; D014162:Transfection
D029742:Caenorhabditis elegans Proteins; D003598:Cytoskeletal Proteins; D043562:Receptors, G-Protein-Coupled; D051193:Toll-Like Receptors; C485906:tir-1 protein, C elegans