Fetal sex determination of macaque monkeys by a nested PCR using maternal plasma.
Non-invasive fetal sex determination is required for biomedical studies, in which some sexual difference would be expected in fetal events, in order to make a choice of male or female fetus. To detect male fetal DNA of the sex-determining region Y gene (SRY) in maternal macaque plasma, nested real-time PCR using the SYBR Green system was developed. In all cases of pregnant macaques with male fetuses, a nested PCR product of SRY was amplified from the mother's plasma, while no amplicon was detected in any case of pregnancy with a female fetus. Interestingly, fetal SRY DNA appeared to be cleared rapidly from the maternal blood after parturition. The current method is sensitive and can be performed with a regular PCR machine.
Mitsunaga
Fusako
F
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan.
Ueiwa
Miyuki
M
Kamanaka
Yoshirou
Y
Morimoto
Mayumi
M
Nakamura
Shin
S
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000097:blood; D005260:Female; D024282:Genes, sry; D008253:Macaca mulatta / Q000502:physiology*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011247:Pregnancy / Q000097:blood* / Q000235:genetics; D011296:Prenatal Diagnosis / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012732:Sex Determination Analysis / Q000379:methods / Q000662:veterinary*
D004247:DNA