The effects of polymicrobial sepsis with diabetes mellitus on kidney tissues in ovariectomized rats.
OBJECTIVE
Sepsis model was used to understand the role of sustained hyperglycemia and ovariectomy, either separately or concomitantly, on the response of the activity of the nuclear factor kappa B (NF-kappaB) and the oxidative response in kidney.


METHODS
Polymicrobial sepsis was induced by cecal ligation and puncture (CLP). Diabetes was induced in female rats using administration of alloxan. The rats were divided into five groups: sham control (group 1), ovariectomy (group 2), ovariectomy + sepsis (group 3), ovariectomy + diabetes (group 4), and ovariectomy + diabetic + sepsis (group 5).


RESULTS
In kidney tissues, the levels of lipid peroxidation (LPO) and glutathione (GSH) and the activity of catalase (CAT) were higher for groups 3, 4, 5 than the control groups. Superoxide dismutase (SOD) activity was lower for groups 3, 4, 5 than the control groups. We determined that CLP produced injury evident in the kidneys of rats when compared to the control group, whereas the severity of the injury was higher in the diabetes + ovariectomy + CLP group when compared to the CLP group. In immunohistochemical staining, we determined that CLP operation increased NF-kappaB activation. In the ovariectomized, septic, and diabetic group, NF-kappaB activation was significantly higher than other groups.


CONCLUSIONS
Hyperglycemia and ovariectomy severely increased NF-kappaB activation and oxidant levels with the stages of our sepsis model. Ovariectomy resulted in general changes in metabolism, which are seen in the kidney with diabetes under sepsis conditions.
Uyanik
Abdullah
A
Division of Nephrology, Department of Internal Medicine, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey.
Unal
Deniz
D
Uyanik
M Hamidullah
MH
Halici
Zekai
Z
Odabasoglu
Fehmi
F
Altunkaynak
Zuhal Berrin
ZB
Cadirci
Elif
E
Keles
Mustafa
M
Gundogdu
Cemal
C
Suleyman
Halis
H
Bayir
Yasin
Y
Albayrak
Mevlut
M
Unal
Bunyami
B
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001806:Blood Urea Nitrogen; D001835:Body Weight; D003404:Creatinine / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D005260:Female; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism*; D010052:Ovariectomy; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000150:complications / Q000378:metabolism* / Q000473:pathology
D000975:Antioxidants; D001786:Blood Glucose; D016328:NF-kappa B; D003404:Creatinine