title: Maturation and survival of Cronobacter biofilms on silicone, polycarbonate, and stainless steel after UV light and ethanol immersion treatments.
abstract: Cronobacter sakazakii cells in biofilms formed on silicone, polycarbonate, and stainless steel coupons immersed in reconstituted powdered infant milk formula were treated with ethanol (10 to 70%) and UV light (12 to 2,160 mW.s/cm(2)) as antibacterial treatments. Biofilm maturation curves were determined after immersion at 25 degrees C for up to 144 h. Populations increased after subsequent immersion at 25 degrees C for 24 h in reconstituted powdered infant milk formula to the respective maximum levels of 7.96, 7.91, and 6.99 log CFU per coupon. Populations attached to silicone and polycarbonate surfaces to a greater extent than to stainless steel (P < 0.05). Treatment with 10% ethanol did not cause a significant decrease in the level of C. sakazakii, but treatment with 30, 40, and 50% ethanol reduced the levels to approximately 1.73, 3.02, and 4.17 log CFU per coupon, respectively. C. sakazakii was not detected on any coupon after treatment with 70% ethanol or 2,160 mW.s/cm(2) UV light. A synergistic effect of sequential ethanol and UV treatments was not observed.
authors.affiliation: Department of Food Science and Technology, Chung-Ang University, 72-1 Naeri, Ansung, Gyunggi-do 456-756, South Korea.
authors.forename: Seo-Hee
authors.initials: SH
authors.lastname: Jo
authors.forename: Seung-Bum
authors.initials: SB
authors.lastname: Baek
authors.forename: Ji-Hyoung
authors.initials: JH
authors.lastname: Ha
authors.forename: Sang-Do
authors.initials: SD
authors.lastname: Ha
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D044083:Cronobacter sakazakii / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D000431:Ethanol / Q000494:pharmacology*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000382:microbiology*; D041943:Infant Formula; D007231:Infant, Newborn; D011076:Polycarboxylate Cement; D012828:Silicones; D013193:Stainless Steel; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D011076:Polycarboxylate Cement; D012828:Silicones; D013193:Stainless Steel; C005506:polycarbonate; D000431:Ethanol