Strain-specific susceptibility for pulmonary metastasis of sarcoma 180 cells in inbred mice.
Most cancer deaths are a result of metastasis. To extend our understanding of the factors that influence the process, we aimed to develop a mouse model of pulmonary metastasis that can be assayed in multiple inbred mouse strains for further use in identification of host genetic variants that influence metastasis. We used i.v. injection of Sarcoma 180 (S180) cells, which can be tracked and quantified by bioluminescence imaging. We observed growth of S180 cells solely in the lung and observed a wide range of pulmonary metastasis among inbred mouse strains. Interestingly, we noted that the BTBRT+tf/J strain exhibited complete clearance and provide evidence that the mechanism of resistance may involve immune factors, as strains subjected to whole-body irradiation are significantly more susceptible to tumor growth. One possible mechanism of resistance to pulmonary metastasis in BTBRT+tf/J mice may require T-cell function. Our experiments present a new mouse model for further characterization of the genetics and mechanisms of pulmonary metastasis.
Vikis
Haris G
HG
Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Jackson
Erin N
EN
Krupnick
Alexander S
AS
Franklin
Andrew
A
Gelman
Andrew E
AE
Chen
Qiong
Q
Piwnica-Worms
David
D
You
Ming
M
D000818:Animals; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D005260:Female; D005434:Flow Cytometry; D008175:Lung Neoplasms / Q000276:immunology / Q000556:secondary*; D008212:Lymphocyte Depletion; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D012510:Sarcoma 180 / Q000276:immunology / Q000556:secondary*; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D014916:Whole-Body Irradiation