title: One naive T cell, multiple fates in CD8+ T cell differentiation.
abstract: The mechanism by which the immune system produces effector and memory T cells is largely unclear. To allow a large-scale assessment of the development of single naive T cells into different subsets, we have developed a technology that introduces unique genetic tags (barcodes) into naive T cells. By comparing the barcodes present in antigen-specific effector and memory T cell populations in systemic and local infection models, at different anatomical sites, and for TCR-pMHC interactions of different avidities, we demonstrate that under all conditions tested, individual naive T cells yield both effector and memory CD8+ T cell progeny. This indicates that effector and memory fate decisions are not determined by the nature of the priming antigen-presenting cell or the time of T cell priming. Instead, for both low and high avidity T cells, individual naive T cells have multiple fates and can differentiate into effector and memory T cell subsets.
authors.affiliation: Division of Immunology, Central Microarray Facility, and Bioinformatics and Statistics Group, The Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
authors.forename: Carmen
authors.initials: C
authors.lastname: Gerlach
authors.forename: Jeroen W J
authors.initials: JW
authors.lastname: van Heijst
authors.forename: Erwin
authors.initials: E
authors.lastname: Swart
authors.forename: Daoud
authors.initials: D
authors.lastname: Sie
authors.forename: Nicola
authors.initials: N
authors.lastname: Armstrong
authors.forename: Ron M
authors.initials: RM
authors.lastname: Kerkhoven
authors.forename: Dietmar
authors.initials: D
authors.lastname: Zehn
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bevan
authors.forename: Koen
authors.initials: K
authors.lastname: Schepers
authors.forename: Ton N M
authors.initials: TN
authors.lastname: Schumacher
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D002454:Cell Differentiation / Q000276:immunology*; D019070:Cell Lineage / Q000276:immunology*; D007156:Immunologic Memory / Q000276:immunology; D008088:Listeriosis / Q000150:complications / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000150:complications / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology
chemical_list: D011948:Receptors, Antigen, T-Cell