title: The heterogeneity of cell subtypes from a primary culture of human amniotic fluid.
abstract: Heterogeneous human amniotic fluid contains various cell types. Herein, we report on the possibility of simultaneously isolating three subtypes of cells from one primary culture. Using a stainless steel instrument named a colony poculum, two of the three cell subtypes could be efficiently cultured, and these were further characterized. The results indicated that these two cell subtypes had different morphologies and were characterized by different cell marker expression profiles, including the differential expression of CD105, CD117 and EBAF. Furthermore, their gene expression array data revealed their different gene expression profiles. Although both cell types expressed several embryonic stem cell-specific markers, they were non-tumorigenic in vivo. This paper not only provides new insight into the heterogeneity of human amniotic fluid, it also presents a simple yet efficient cell isolation method. These results will contribute to the thorough investigation of the properties and potential future applications of human amniotic fluid-derived cells.
authors.affiliation: Department of Urology, Shanghai Children's Hospital, Shanghai Jiao Tong University School of Medicine, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Zhang
authors.forename: Hongquan
authors.initials: H
authors.lastname: Geng
authors.forename: Hua
authors.initials: H
authors.lastname: Xie
authors.forename: Qiquan
authors.initials: Q
authors.lastname: Wu
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Ma
authors.forename: Junmei
authors.initials: J
authors.lastname: Zhou
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D000071063:Endoglin; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D007621:Karyotyping; D055458:Left-Right Determination Factors / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; C064206:ENG protein, human; D000071063:Endoglin; C411603:LEFTY2 protein, human; D055458:Left-Right Determination Factors; D011956:Receptors, Cell Surface; D019009:Proto-Oncogene Proteins c-kit