[Changes of aquaporin-1 expression in rat myocardium after severe burn].
OBJECTIVE
To investigate the changes in the myocardial expression of aquaporin-1 (AQP1) protein and its association with myocardial edema in rats with severe burns.


METHODS
Forty-eight healthy adult Wistar rats were randomly divided into normal control group (n=6) and burn injury group with third degree burn of 30% total body surface area, and the latter group was further divided into 2, 4, 8, 12, 24, 48 and 72 h groups. The changes of myocardial water content were investigated by dry-wet weight methods. Enzyme-linked immunosorbent assay was used to detect the changes in AQP1 expression at different time points after sever burns.


RESULTS
The myocardial water content and AQP1 expression increased significantly 2 h after the burn injury, reaching the peak levels at 12 h and remaining higher than the normal level at 48 h. A significant positive correlation was found between myocardial water content and AQP1 expression in the rats (r=0.868, P<0.01).


CONCLUSIONS
The severity of myocardial edema after severe burn is correlated to the expression level of AQPl protein, suggesting the important role of AQPl protein in pathological progression of myocardial edema.
Xiao
De-quan
DQ
Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. xiaodequan80@163.com
Wang
Jia-han
JH
Li
Zhi-qing
ZQ
Yi
Zhao-hui
ZH
Xu
Li Xiao-zi
LX
D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D004487:Edema / Q000378:metabolism; D005260:Female; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
C496022:Aqp1 protein, rat; D051398:Aquaporin 1