title: Cocirculation of Rio Negro Virus (RNV) and Pixuna Virus (PIXV) in Tucumán province, Argentina.
abstract: Venezuelan equine encephalitis complex includes viruses considered emerging pathogens for humans and animals in the Americas. Two members of this complex have been detected previously in Argentina: Rio Negro Virus (RNV), detected in mosquitoes from Chaco province and rodents from Formosa province, and Pixuna Virus (PIXV), detected in mosquitoes from Chaco province. To carry out surveillance studies in other parts of the country, detection of a 195-bp fragment of alphaviruses by RT-nested PCR was performed in mosquito samples from San Miguel de Tucumán city. Four pools resulted positive and three were sequenced. Two amplicons grouped with RNV and one with PIXV. This is the first report of viral activity of members of the Venezuelan equine encephalitis complex in north-eastern Argentina.
authors.affiliation: Instituto de Virología Dr. J.M. Vanella, Facultad Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina. pisanomb@yahoo.com
authors.forename: M B
authors.initials: MB
authors.lastname: Pisano
authors.forename: M J
authors.initials: MJ
authors.lastname: Dantur
authors.forename: V E
authors.initials: VE
authors.lastname: Ré
authors.forename: L A
authors.initials: LA
authors.lastname: Díaz
authors.forename: A
authors.initials: A
authors.lastname: Farías
authors.forename: M P
authors.initials: MP
authors.lastname: Sánchez Seco
authors.forename: A
authors.initials: A
authors.lastname: Tenorio
authors.forename: W R
authors.initials: WR
authors.lastname: Almirón
authors.forename: M S
authors.initials: MS
authors.lastname: Contigiani
mesh_terms: D000524:Alphavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001118:Argentina; D009033:Culicidae / Q000821:virology*; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics / Q000302:isolation & purification; D007303:Insect Vectors / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity