Retinoic acid and dimethyl sulfoxide promote efficient delivery of transgenes to mouse skin by topically transdermal penetration.
Simple and efficient gene transfer to the skin would facilitate many local and systemic gene therapy applications. This study reports a novel approach that allows expression of plasmid DNA in epidermis and hair follicle cells with dimethyl sulfoxide (DMSO) after pre-treatment with depilation and retinoic acid (RA) for the purposes of gene therapy. This study investigated the transdermal efficacy of gene to mouse skin when utilizing DMSO after RA pre-treatment. Retinoic acid pre-treatment can increase the efficiency of transfection. This finding indicates that one can more effectively and much less expensively make use of genes therapy to treat diseases of the hair and skin.
Chen
Xiangjun
X
State Key Laboratory of Biotherapy,West China Hospital, Sichuan University, Chengdu,PR China.
Zhang
Yinbing
Y
Liu
Changyong
C
Zhang
Yan
Y
Zhou
Xikun
X
Zhou
Taoyou
T
Mao
Yongqiu
Y
Kan
Bing
B
Wei
Yu-Quan
YQ
Li
Jiong
J
D000279:Administration, Cutaneous; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage / Q000737:chemistry*; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000191:economics / Q000379:methods*; D018859:Hair Follicle / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009994:Osmolar Concentration; D010539:Permeability / Q000187:drug effects; D014677:Pharmaceutical Vehicles; D010957:Plasmids / Q000097:blood / Q000235:genetics / Q000493:pharmacokinetics; D011292:Premedication; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012871:Skin Diseases / Q000628:therapy; D014162:Transfection / Q000379:methods*; D019076:Transgenes; D014212:Tretinoin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D014677:Pharmaceutical Vehicles; D014212:Tretinoin; D004121:Dimethyl Sulfoxide