[Quantitative analysis of aglycone of momordicoside L from Momordica charantia in different areas by HPLC].
OBJECTIVE
To develop an HPLC for determination of aglycone of momordicoside L in Momordica charantia.


METHODS
A Kromasil C18 (4.6 mm x 150 mm, 5 microm) column was used. The mobile phase was acetonitrile-H2O (64:36), the flow rate was 1.0 mL x min(-1) and the UV detection wavelength was set 203 nm.


RESULTS
The calibration curves were linear from of 0.025 microg to 1 microg (r =0.9911), the contents of aglycone of momordicoside L in Shandong, Henan, Hebei, Jiangxi are 0.211, 0.033, 0.013, 0.007 mg x g(-1), respectively.


CONCLUSIONS
The method is simple and reliable for determination of aglycone of momordicoside L in M. charantia.
Zhang
Yu
Y
Yanbian University, Yanbian 133000, China.
Guan
Jian
J
Cui
Jiongmo
J
Zhao
Yuqing
Y
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029777:Momordica charantia / Q000737:chemistry*; D012503:Saponins / Q000032:analysis*
D012503:Saponins; C522769:momordicoside L