Renal oncocytoma: a comparative clinicopathologic study and fluorescent in-situ hybridization analysis of 73 cases with long-term follow-up.
Clinical studies have confirmed that renal oncocytoma (RO) is a benign neoplasm with excellent prognosis. In diagnostically challenging cases of renal oncocytic epithelial neoplasms, fluorescent in-situ hybridization (FISH) is increasingly being used and its ability to distinguish RO from chromophobe renal cell carcinoma (ChRCC) has been documented. In this study, we evaluated the differential diagnostic contribution of FISH in cases of RO.Clinicopathologic data and glass slides from 73 patients with RO were reviewed; 20 cases of ChRCC were included for comparison. FISH analysis of formalin-fixed, paraffin-embedded sections was performed using centromeric probes for chromosomes 1, 2, 7 and 17. FISH analysis revealed ROs had frequent loss of signal for chromosome 1 (56%) and 17 (44%). Tumors with more than one loss were common (41%) and 10% cases showed loss of all chromosomes examined. A total of 18% cases did not show any abnormality.Our study shows that chromosomal abnormalities in both ROs and ChRCCs are common with frequent loss of chromosomes 1 and 17. No association was found between overall patient survival and the extent of chromosomal abnormalities. FISH results, even those showing significant chromosomal abnormalities, should not alter the primarily morphology-based diagnosis of RO.
Dvorakova
Marie
M
Department of Pathology, UPMC Shadyside, 5230 Centre Avenue, Pittsburgh, PA 15232, USA.
Dhir
Rajiv
R
Bastacky
Sheldon I
SI
Cieply
Kathleen M
KM
Acquafondata
Marie B
MB
Sherer
Carol R
CR
Mercuri
Tracy L
TL
Parwani
Anil V
AV
D018249:Adenoma, Oxyphilic / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002886:Chromosomes, Human, Pair 17; D002889:Chromosomes, Human, Pair 2; D005260:Female; D005404:Fixatives; D005500:Follow-Up Studies; D005557:Formaldehyde; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007680:Kidney Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009392:Nephrectomy; D016612:Paraffin Embedding; D011237:Predictive Value of Tests; D013997:Time Factors; D016707:Tissue Fixation; D016896:Treatment Outcome
D005404:Fixatives; D005557:Formaldehyde