Gene expression profiles and microsatellite instability in uterine corpus endometrioid adenocarcinoma.
OBJECTIVE
We examined corpus cancer to identify whether there are distinctive patterns of global gene expression and microsatellite instability, and to gain molecular understanding of its carcinogenesis and progression.


METHODS
Thirty endometrioid corpus cancer tissue samples (21 of G1 and nine of G2/3) were analyzed by cDNA microarray based on 637 cancer-associated genes and by a polymerase chain reaction method for microsatellite instability.


RESULTS
Of the 30 cases, 10 (33%) were recognized as having microsatellite instability. In all cases, four genes were overexpressed and five genes were underexpressed. There were six microsatellite instability-specific overexpressed or high-frequency genes and 15 underexpressed or low-frequency genes. Furthermore, we identified several genes by grade analysis.


CONCLUSIONS
These results may be useful resources for the development of diagnostic assays, prognostic factors, or therapeutic targets for corpus endometrioid cancer.
Koyamatsu
Yasuko
Y
Department of Gynecology, Sasaki Foundation Kyoundo Hospital, 1-8, Kandasurugadai, Chiyodaku, Tokyo 101-0034, Japan. y.koya@t-t-t.jp
Sakamoto
Masaru
M
Miyake
Kiyohiko
K
Muroya
Tetsuya
T
Sugano
Kokichi
K
Nakao
Yoshifumi
Y
Yokoyama
Masatoshi
M
Iwasaka
Tsuyoshi
T
D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D018269:Carcinoma, Endometrioid / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016009:Chi-Square Distribution; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053842:Microsatellite Instability; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
D014408:Biomarkers, Tumor; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D014746:Vimentin