title: Quantifying cross-tissue diversity in proteasome complexes by mass spectrometry.
abstract: The composition of 20S mouse proteasome complexes isolated from mice heart, kidney, liver, lung, thymus and spleen was compared using quantitative mass spectrometry. Significant variety was observed in hybrid classes of immunoproteasomes which may have implications for the use of proteasome targeted inhibitors.
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, Utrecht, The Netherlands.
authors.forename: Sarah
authors.initials: S
authors.lastname: Pelletier
authors.forename: Karianne G
authors.initials: KG
authors.lastname: Schuurman
authors.forename: Celia R
authors.initials: CR
authors.lastname: Berkers
authors.forename: Huib
authors.initials: H
authors.lastname: Ovaa
authors.forename: Albert J R
authors.initials: AJ
authors.lastname: Heck
authors.forename: Reinout
authors.initials: R
authors.lastname: Raijmakers
mesh_terms: D000818:Animals; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008954:Models, Biological; D009097:Multienzyme Complexes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D009928:Organ Specificity; D046988:Proteasome Endopeptidase Complex / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D021122:Protein Subunits / Q000032:analysis; D013154:Spleen / Q000737:chemistry / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D009097:Multienzyme Complexes; D021122:Protein Subunits; D046988:Proteasome Endopeptidase Complex