title: [Changes of aquaporin-1 expression in rat myocardium after severe burn].
abstract: OBJECTIVE
  To investigate the changes in the myocardial expression of aquaporin-1 (AQP1) protein and its association with myocardial edema in rats with severe burns.
  
  
  METHODS
  Forty-eight healthy adult Wistar rats were randomly divided into normal control group (n=6) and burn injury group with third degree burn of 30% total body surface area, and the latter group was further divided into 2, 4, 8, 12, 24, 48 and 72 h groups. The changes of myocardial water content were investigated by dry-wet weight methods. Enzyme-linked immunosorbent assay was used to detect the changes in AQP1 expression at different time points after sever burns.
  
  
  RESULTS
  The myocardial water content and AQP1 expression increased significantly 2 h after the burn injury, reaching the peak levels at 12 h and remaining higher than the normal level at 48 h. A significant positive correlation was found between myocardial water content and AQP1 expression in the rats (r=0.868, P<0.01).
  
  
  CONCLUSIONS
  The severity of myocardial edema after severe burn is correlated to the expression level of AQPl protein, suggesting the important role of AQPl protein in pathological progression of myocardial edema.
authors.affiliation: Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. xiaodequan80@163.com
authors.forename: De-quan
authors.initials: DQ
authors.lastname: Xiao
authors.forename: Jia-han
authors.initials: JH
authors.lastname: Wang
authors.forename: Zhi-qing
authors.initials: ZQ
authors.lastname: Li
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Yi
authors.forename: Li Xiao-zi
authors.initials: LX
authors.lastname: Xu
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D004487:Edema / Q000378:metabolism; D005260:Female; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: C496022:Aqp1 protein, rat; D051398:Aquaporin 1