title: Antiproliferative effect of adiponectin on rat uterine leiomyoma ELT-3 cells.
abstract: OBJECTIVE
  Although fibroids greatly affect reproductive health, the pathophysiology and epidemiology are not well known. Recently, we have reported the relationship between uterine leiomyoma and metabolic syndrome. Many studies have indicated that reductions in plasma adiponectin levels play major roles in the development of metabolic syndrome. In this study, we investigated the significant repressive effect of adiponectin on rat uterine leiomyoma ELT-3 cells proliferation.
  
  
  METHODS
  Expression of adiponectin receptor 1 and receptor 2 was evaluated by RT-PCR and Western blot analysis. Cell proliferation was assessed by the MTS assay and cell counting. Apoptosis was evaluated by Hoechst staining and cleaved poly (ADP-ribose) polymerase (PARP).
  
  
  RESULTS
  Adiponectin receptor 1 and receptor 2 were expressed in ELT-3 cells. Adiponectin repressed rat uterine leiomyoma ELT-3 cells cell proliferation without inducing apoptosis.
  
  
  CONCLUSIONS
  The repression of adiponectin on leiomyoma cell proliferation in the rat may explain a crucial role of adiponectin in the association of metabolic syndrome with uterine leiomyoma.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Wakabayashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Takeda
authors.forename: Kenji
authors.initials: K
authors.lastname: Tsuiji
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakata
authors.forename: Ken-ichirou
authors.initials: K
authors.lastname: Morishige
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yaegashi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kimura
mesh_terms: D052242:Adiponectin / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007889:Leiomyoma / Q000737:chemistry / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D054419:Receptors, Adiponectin / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014594:Uterine Neoplasms / Q000737:chemistry / Q000473:pathology*
chemical_list: D052242:Adiponectin; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; C504339:adiponectin receptor 1, rat; C506573:adiponectin receptor 2, rat