title: [Primary cell culture and identification methods of rat pulmonary arterial smooth muscle cells].
abstract: OBJECTIVE
  Set up a method to isolate and identify the small pulmonary arterial smooth muscle cells (PASMCs) in vitro.
  
  
  METHODS
  In sterile conditions, separated the male SD rat pulmonary artery, digested by collagenase I and cultured primary PASMCs. Measured cell viability; observed by phase contrast microscope; identified by immunocytochemistry and immunofluorescence staining as a label for smooth muscle alpha-actin.
  
  
  RESULTS
  PASMCs were identified by morphology and immunocytochemistry, immunofluorescence staining, with the cell viability is over 96.5%. The primary culture could be subcultured after 4-7 days and successfully passaged without change in morphology and growth characteristic.
  
  
  CONCLUSIONS
  This technique has advantage of the method is simple, short cultivate, good reproducibility, the primary cultured PASMCs quantity and the rapid growth.
authors.affiliation: Department of Respiratory, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: Qian
authors.forename: Liang-xing
authors.initials: LX
authors.lastname: Wang
authors.forename: Chan
authors.initials: C
authors.lastname: Cheng
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Huang
authors.forename: Shu-ting
authors.initials: ST
authors.lastname: Yei
mesh_terms: D000818:Animals; D001160:Arterioles / Q000166:cytology; D002469:Cell Separation / Q000379:methods; D008168:Lung / Q000098:blood supply; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D061251:Primary Cell Culture / Q000379:methods*; D011651:Pulmonary Artery / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley