Mutual inhibition of incorporation into protein of branched-chain amino acids in brain homogenates of developing and adult rats.
The mutual inhibitory effect of an excess of leucine, isoleucine and valine on their incorporation into protein was studied with brain homogenates prepared from 1-, 7-, 14- and 90-day-old rats. All these branched-chain amino acids diminished each other's incorporation, leucine being slightly more potent in this respect. The inhibition was the stronger the younger were the experimental animals. An attempt was made to analyze with a digital computer the kinetics of inhibition of incorporation by fitting the experimental data by the Hill equation ?/V = s(n)/(K + s(n)). Mutual competition appears to occur, but the maximal velocity of incorporation was also in many cases diminished by these amino acids.
Pajari
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Department of Biomedical Sciences, University of Tampere, Box 607, SF-33101 Tampere 10, Finland.
Oja
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