title: Reduced thrombocyte adhesion to endothelialized poly 4-methyl-1-pentene gas exchange membranes—a first step toward bioartificial lung development.
abstract: Polymeric materials used in biomedical devices, bioartificial organs, or for the fabrication of tissue engineering scaffolds should completely prevent the activation of the coagulation system and subsequent clot formation. Surface endothelialization is considered an important tool to optimize the blood compatibility of synthetic materials, as a functional endothelial cell layer on an artificial material may help control hemostasis and, therefore, provide a solution to improve the biocompatibility of these materials. Here we report on the endothelialization of poly 4-methyl-1-pentene (PMP) gas exchange membranes using human cord blood-derived late outgrowth endothelial colony forming cells. We achieved complete endothelialization of PMP membranes; and when seeded and cultivated on the membrane, cord blood-derived late outgrowth endothelial colony forming cells maintained both endothelial characteristics and functionality. Endothelialization resulted in significantly lower platelet adhesion and activation compared with unseeded membranes. Of importance, the endothelial layer had no major impact on gas permeability of PMP membranes. This study is a first promising step toward the development of a biofunctionalized surface for the use in gas exchange devices with blood contacting surfaces and a straightforward approach toward a long-term bio-hybrid lung replacement system.
authors.affiliation: Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Hannover, Germany. hess.christian@mh-hannover.de
authors.forename: Christian
authors.initials: C
authors.lastname: Hess
authors.forename: Bettina
authors.initials: B
authors.lastname: Wiegmann
authors.forename: Andreas N
authors.initials: AN
authors.lastname: Maurer
authors.forename: Philipp
authors.initials: P
authors.lastname: Fischer
authors.forename: Lena
authors.initials: L
authors.lastname: Möller
authors.forename: Ulrich
authors.initials: U
authors.lastname: Martin
authors.forename: Andres
authors.initials: A
authors.lastname: Hilfiker
authors.forename: Axel
authors.initials: A
authors.lastname: Haverich
authors.forename: Stefan
authors.initials: S
authors.lastname: Fischer
mesh_terms: D021522:Bioartificial Organs; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D008168:Lung / Q000166:cytology* / Q000378:metabolism*; D008567:Membranes, Artificial; D015539:Platelet Activation / Q000187:drug effects; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D011659:Pulmonary Gas Exchange / Q000502:physiology*; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; D012333:RNA, Messenger