title: Effects of increased chronic loading on articular cartilage material properties in the lapine tibio-femoral joint.
abstract: Methods of producing relevant and quantifiable load alterations in vivo with which to study load-induced cartilage degeneration analogous to osteoarthritis are limited. An animal model was used to investigate the effects of increased chronic loads on articular cartilage. Mature rabbits were randomized into one of three experimentally loaded groups and a fourth unoperated control group. A mechanical-loading device was skeletally fixed to the hind limb of animals in the loaded groups. Engaging the device resulted in an additional load of 0%, +22% or +44% body weight to the medial compartment of the experimental knee, while allowing normal joint function. Following a 12-week loading protocol, a creep-indentation test and needle probe test were used to determine the biphasic material properties and thickness of the cartilage at four locations of each femoral and tibial condyle of the experimental and contralateral limbs. Analyses of covariance were performed to compare outcome measures across the treatment groups. The effect of increased load was site and load-level specific with alterations of material properties and thickness most prominent in the posterior region of the medial compartment of the tibia. At this site, permeability increased 128% and thickness increased 28% in the +44% body weight group relative to the 0% body weight group. This model of altered chronic loading initiated changes in the material properties to the articular cartilage at the sites of increased load over 12-weeks that were consistent with early degenerative changes suggesting that increased tibio-femoral loading may be responsible for the alterations. This work begins to elucidate the chronic-load threshold and the time course of cartilage degeneration at different levels of altered loading.
authors.affiliation: Department of Orthopaedics and Rehabilitation, 95 Carrigan Drive, University of Vermont, Burlington, VT 05405, USA. maria.roemhildt@med.uvm.edu
authors.forename: Maria L
authors.initials: ML
authors.lastname: Roemhildt
authors.forename: Kathryn M
authors.initials: KM
authors.lastname: Coughlin
authors.forename: Glenn D
authors.initials: GD
authors.lastname: Peura
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Badger
authors.forename: Dave
authors.initials: D
authors.lastname: Churchill
authors.forename: Braden C
authors.initials: BC
authors.lastname: Fleming
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Beynnon
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002358:Cartilage, Articular / Q000503:physiopathology*; D004195:Disease Models, Animal; D055119:Elastic Modulus; D005260:Female; D005269:Femur / Q000503:physiopathology; D006025:Glycosaminoglycans / Q000378:metabolism; D006614:Hindlimb; D007596:Joints / Q000503:physiopathology*; D010003:Osteoarthritis / Q000209:etiology / Q000503:physiopathology; D010539:Permeability; D011817:Rabbits; D013314:Stress, Mechanical; D013977:Tibia / Q000503:physiopathology; D016474:Weight-Bearing / Q000502:physiology
chemical_list: D006025:Glycosaminoglycans