title: Aluminum stress increases carbon-centered radicals in soybean roots.
abstract: The formation of radical species was examined in roots of soybean seedlings exposed to aluminum (Al). Electron spin resonance (ESR) spectra of root homogenates with the spin-trapping reagent 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO) indicated the presence of carbon-centered radicals in plants not exposed to Al. Plants exposed to 50 microM Al showed a similar spectrum, with increased signal intensity. These radicals were likely produced through a H-atom abstraction reaction by hydroxyl (*OH) radicals, the synthesis of which was initiated by the formation of superoxide (O2*-) anions. The increased production of the carbon-centered radicals may be responsible for the lipid peroxidation in Al-treated roots.
authors.affiliation: Department of Applied Biological Chemistry, School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Abo
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yonehara
authors.forename: Etsuro
authors.initials: E
authors.lastname: Yoshimura
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D002244:Carbon / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism*; D011758:Pyrroles / Q000378:metabolism; D012394:Rosaniline Dyes / Q000378:metabolism; D013025:Glycine max / Q000187:drug effects / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: C510912:(diethoxyphosphoryl)-5-methylpyrroline N-oxide; D005609:Free Radicals; D011758:Pyrroles; D012394:Rosaniline Dyes; C476677:Schiff's reagent; D017665:Hydroxyl Radical; D002244:Carbon; D000535:Aluminum