Selective microbial aerosolization in biogas demonstrated by quantitative PCR.
Aerosolization of Bacteria, Archaea, Synergistes, Staphylococcus spp. and Propionibacterium acnes was investigated in situ with quantitative real-time PCR of DNA isolated from sludge and biogases of anaerobic digesters. The data revealed that in biogas, Staphylococcus spp. and P. acnes were, respectively, aerosolized 30 and 220 times more and Archaea and Synergistes, respectively, 8 and 20 times less aerosolized than Bacteria. This is the first demonstration of selective microbial aerosolization for anaerobic digestors microorganisms. This study illustrates the fact that some microbial groups, such as opportunistic pathogens, are more susceptible to be aerosolized, since they use air as a dissemination vector, and that this has to be taken in account when up-grading biogas into natural gas networks.
Moletta-Denat
Marina
M
INRA, UR50, Laboratoire de Biotechnologie de l'Environnement, Narbonne, France. marina.moletta@cstb.fr
Bru-Adan
Valérie
V
Delgenes
Jean-Philippe
JP
Hamelin
Jérôme
J
Wéry
Nathalie
N
Godon
Jean-Jacques
JJ
D000336:Aerosols / Q000032:analysis*; D000693:Anaerobiosis / Q000235:genetics; D001105:Archaea / Q000235:genetics* / Q000302:isolation & purification; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification; D056804:Biofuels / Q000382:microbiology*; D015169:Colony Count, Microbial; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction / Q000379:methods*; D012722:Sewage / Q000382:microbiology
D000336:Aerosols; D056804:Biofuels; D012722:Sewage