Phylogenetic analysis and prokaryotic expression of NMAAP1 derived from BCG-activated murine macrophages.
BCG-activated macrophages exerted anti-tumor activities. Cell surface molecules play an important role in mediating endocytosis by macrophages. In the previous study, we identified a group of 454 membrane proteins specifically expressed on BCG-activated mouse macrophages, including a protein named NMAAP1 (novel macrophage activated associated protein). In this study, we aligned the full-length nucleotide sequences of NMAAP1 and its homologous sequences to construct its phylogenetic tree, and cloned the NMAAP1 cDNA from BCG-activated macrophages to generated NMAAP1 fusion protein in Escherichia coli. Purified the fusion protein were applied for generation of polyclonal antibodies. Western-blotting detection showed that the polyclonal antibodies have high specificities to recognize target protein.
Zhao
Xiangfeng
X
Department of Immunology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China. zxf184@163.com
Zhang
Linbing
L
Yan
Dongmei
D
Feng
Xiuping
X
Chu
Dun
D
Li
Bin
B
Du
Bairong
B
Zhu
Xun
X
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors / Q000235:genetics; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000276:immunology*; D010802:Phylogeny; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
D008565:Membrane Proteins; C561300:NMAAP1 protein, mouse; D011994:Recombinant Proteins