title: Short report: Tissue-specific expression profiles of the uncoupling protein family in normal control mice and genetically ob/ob mice.
abstract: Uncoupling proteins (UCPs) located in the inner mitochondrial membrane are involved in the regulation of energy balance. Thus far, 5 UCP isoforms have been identified, but controversies exist in the research focused on the function of the UCPs (except UCP1) in the pathogenesis of obesity. Because of the known cross-reactivity of the antibodies presently available for the detection of UCP proteins, this study systematically analyzed the differential tissue expression profiles of the 5 UCP isoforms in lean control mice and ob/ob mice by using real-time polymerase chain reaction (PCR) analysis. The results show that the tissue-specific expression patterns of individual isoforms in normal and ob/ob mice are considerably different; this will provide new insights into the functions of UCPs in the pathogenesis of genetic obesity.
authors.affiliation: Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Jin-Gai
authors.initials: JG
authors.lastname: Zhu
authors.forename: Chen-Bo
authors.initials: CB
authors.lastname: Ji
authors.forename: Chun
authors.initials: C
authors.lastname: Zhu
authors.forename: Chun-Zhao
authors.initials: CZ
authors.lastname: Kou
authors.forename: Da-Ni
authors.initials: DN
authors.lastname: Qin
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Tong
authors.forename: Xi-Rong
authors.initials: XR
authors.lastname: Guo
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D007473:Ion Channels / Q000235:genetics*; D051379:Mice; D008820:Mice, Obese; D024101:Mitochondrial Proteins / Q000235:genetics*; D009765:Obesity / Q000209:etiology / Q000235:genetics*; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D000071256:Uncoupling Protein 1
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1