Homologous RBC-derived vesicles as ultrasmall carriers of iron oxide for magnetic resonance imaging of stem cells.
Ultrasmall superparamagnetic iron oxide (USPIO) particles are very useful for cellular magnetic resonance imaging (MRI), which plays a key role in developing successful stem cell therapies. However, their low intracellular labeling efficiency, and biosafety concerns associated with their use, have limited their potential usage. In this study we develop a novel system composed of RBC-derived vesicles (RDVs) for efficient delivery of USPIO particles into human bone marrow mesenchymal stem cells (MSCs) for cellular MRI in vitro and in vivo. RDVs are highly biosafe to their autologous MSCs as manifested by cell viability, differentiation, and gene microarray assays. The data demonstrate the potential of RDVs as intracellular delivery vehicles for biomedical applications.
Chang
Microsugar
M
Center for Nanomedicine Research, National Health Research Institutes, Zhunan, Miaoli County, Taiwan.
Hsiao
Jong-Kai
JK
Yao
Ming
M
Chien
Li-Ying
LY
Hsu
Szu-Chun
SC
Ko
Bor-Sheng
BS
Chen
Shin-Tai
ST
Liu
Hon-Man
HM
Chen
Yao-Chang
YC
Yang
Chung-Shi
CS
Huang
Dong-Ming
DM
D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology; D005290:Ferric Compounds / Q000008:administration & dosage*; D015870:Gene Expression; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
D005290:Ferric Compounds; C000499:ferric oxide