Compressive force stimulates the gene expression of IL-17s and their receptors in MC3T3-E1 cells.
During orthodontic tooth movement, cytokines released from periodontal ligament fibroblasts and alveolar bone osteoblasts can alter the process of bone remodeling. Recently, interleukin-17 (IL-17) was found to stimulate osteoclastic resorption through osteoblasts by inducing receptor activator of nuclear factor κB ligand (RANKL) expression. However, the relationship between mechanical stress and IL-17 production by osteoblasts is not clear. Therefore, we examined the effect of compressive force on the expressions of IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, IL-17F, and their receptors (IL-17RA, IL-17RB, IL-17RC, IL-17RD, and IL-17RE) using MC3T3-E1 cells as osteoblast-like cells. We also examined the effect of IL-17A on the expression of IL-17Rs, RANKL, macrophage colony-stimulating factor (M-CSF), and osteoprotegerin (OPG). The cells were cultured with or without continuous compressive force (1.0 and 2.0 g/cm(2)) for up to 24 hr. The cells were also cultured with or without IL-17A (0.1, 1.0, or 10 ng/ml) for up to 72 hr. The mRNA expressions of IL-17s and their receptors were estimated by real-time polymerase chain reaction. The expression levels of IL-17s and their receptors increased depending on the compressive force. The addition of IL-17A increased the expression of IL-17RA, IL-17RB, IL-17RC, IL-17RE, RANKL, and M-CSF, whereas it decreased OPG expression. These results indicate that compressive force induces the expression of IL-17s and their receptors in osteoblast-like cells and that IL-17s and their receptors produced in response to compressive force may affect osteoclastogenesis through the expression of RANKL, M-CSF, and OPG.
Zhang
Fan
F
Department of Orthodontics, Shandong University School of Dentistry, Jinan, Shandong Province, China.
Wang
Chun-Ling
CL
Koyama
Yuki
Y
Mitsui
Narihiro
N
Shionome
Chieko
C
Sanuki
Rina
R
Suzuki
Naoto
N
Mayahara
Kotoe
K
Shimizu
Noriyoshi
N
Maeno
Masao
M
D016475:3T3 Cells; D000818:Animals; D019245:Compressive Strength / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000235:genetics; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D053244:Osteoprotegerin / Q000096:biosynthesis / Q000235:genetics; D053245:RANK Ligand / Q000096:biosynthesis / Q000235:genetics; D053722:Receptors, Interleukin-17 / Q000096:biosynthesis* / Q000235:genetics; D013314:Stress, Mechanical
D020381:Interleukin-17; D053244:Osteoprotegerin; D053245:RANK Ligand; D053722:Receptors, Interleukin-17; D016173:Macrophage Colony-Stimulating Factor