[Simultaneous determination of four active components in Spica Prunellae by HPLC].
OBJECTIVE
To develop a HPLC method for simultaneous determination of caffeic acid, rosmarinic acid, oleanolic acid and ursolic acid in Spica Prunellae.


METHODS
After ultrasonic extraction with 75% ethanol solution containing 1% formic acid, the ethanol-extract of Spica Prunellae was analyzed on an Elite SinoChrom ODS-AP column using gradient elution of 0.01% phosphoric acid (A) and acetonitrile (B) at a flow rate of 0.9-1.0 mL x min(-1). A wavelength switch program was used for detection at 330 nm (0-33 min) and 203 nm (33-40 min). The column temperature was set at 20 degrees C and injection volume was 50 microL.


RESULTS
The calibration curves of all analytes were linear. The average recoveries were 93.7%-105.2% with RSDs not more than 4.5%. The contents of caffeic acid, rosmarinic acid, ursolic acid and oleanolic acid in Spica Prunellae were 0.0401-0.0968, 0.99-2.57, 0.243-0.556, 4.06-8.13 mg x g(-1), respectively.


CONCLUSIONS
The described method is sensitive, convenient and accurate, and is suitable for the simultaneous determination of caffeic acid, rosmarinic acid, oleanolic acid and ursolic acid in Spica Prunellae.
Fang
Luo
L
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. leofang6000@gmail.com
Lin
Nengming
N
Wu
Yongjiang
Y
D002109:Caffeic Acids / Q000032:analysis; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis; D053630:Depsides / Q000032:analysis; D009828:Oleanolic Acid / Q000032:analysis; D031347:Prunella / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis; D000097123:Rosmarinic Acid; D000097245:Ursolic Acid
D002109:Caffeic Acids; D002934:Cinnamates; D053630:Depsides; D009828:Oleanolic Acid; D014315:Triterpenes; D000097123:Rosmarinic Acid; D000097245:Ursolic Acid; C040048:caffeic acid