title: Sugar transporters of the major facilitator superfamily in aphids; from gene prediction to functional characterization.
abstract: Analysis of the pea aphid (Acyrthosiphon pisum) genome using signatures specific to the Major Facilitator Superfamily (Pfam Clan CL0015) and the Sugar_tr family (Pfam Family PF00083) has identified 54 genes encoding potential sugar transporters, of which 38 have corresponding ESTs. Twenty-nine genes contain the InterPro IPR003663 hexose transporter signature. The protein encoded by Ap_ST3, the most abundantly expressed sugar transporter gene, was functionally characterized by expression as a recombinant protein. Ap_ST3 acts as a low-affinity uniporter for fructose and glucose that does not depend on Na(+) or H(+) for activity. Ap_ST3 was expressed at elevated levels in distal gut tissue, consistent with a role in gut sugar transport. The A. pisum genome shows evidence of duplications of sugar transporter genes.
authors.affiliation: Durham University, UK.
authors.forename: D R G
authors.initials: DR
authors.lastname: Price
authors.forename: K
authors.initials: K
authors.lastname: Tibbles
authors.forename: S
authors.initials: S
authors.lastname: Shigenobu
authors.forename: A
authors.initials: A
authors.lastname: Smertenko
authors.forename: C W
authors.initials: CW
authors.lastname: Russell
authors.forename: A E
authors.initials: AE
authors.lastname: Douglas
authors.forename: E
authors.initials: E
authors.lastname: Fitches
authors.forename: A M R
authors.initials: AM
authors.lastname: Gatehouse
authors.forename: J A
authors.initials: JA
authors.lastname: Gatehouse
mesh_terms: D000818:Animals; D001042:Aphids / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D005632:Fructose / Q000378:metabolism; D020440:Gene Duplication; D017344:Genes, Insect; D049750:Genome, Insect; D005947:Glucose / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D005810:Multigene Family; D018532:Pisum sativum / Q000469:parasitology; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D019476:Insect Proteins; D009004:Monosaccharide Transport Proteins; D011994:Recombinant Proteins; D005632:Fructose; D005947:Glucose