Systematic comparison of constitutive promoters and the doxycycline-inducible promoter.
Constitutive promoters are used routinely to drive ectopic gene expression. Here, we carried out a systematic comparison of eight commonly used constitutive promoters (SV40, CMV, UBC, EF1A, PGK and CAGG for mammalian systems, and COPIA and ACT5C for Drosophila systems). We also included in the comparison the TRE promoter, which can be activated by the rtTA transcriptional activator in a doxycycline-inducible manner. To make our findings representative, we conducted the comparison in a variety of cell types derived from several species. We found that these promoters vary considerably from one another in their strength. Most promoters have fairly consistent strengths across different cell types, but the CMV promoter can vary considerably from cell type to cell type. At maximal induction, the TRE promoter is comparable to a strong constitutive promoter. These results should facilitate more rational choices of promoters in ectopic gene expression studies.
Qin
Jane Yuxia
JY
School of Life Sciences, Northeast Normal University, Changchun, China.
Zhang
Li
L
Clift
Kayla L
KL
Hulur
Imge
I
Xiang
Andy Peng
AP
Ren
Bing-Zhong
BZ
Lahn
Bruce T
BT
D000818:Animals; D002460:Cell Line; D004318:Doxycycline / Q000494:pharmacology*; D004331:Drosophila melanogaster / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D005434:Flow Cytometry; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000187:drug effects / Q000235:genetics; D051379:Mice; D011401:Promoter Regions, Genetic; D020218:Response Elements / Q000235:genetics; D013752:Tetracycline / Q000494:pharmacology; D014161:Transduction, Genetic
D049452:Green Fluorescent Proteins; D013752:Tetracycline; D004318:Doxycycline