title: A pilot study of mRNA expressions of 5-fluorouracil pathway genes in peripheral blood mononuclear cells and tumor tissues in patients with lung adenocarcinoma.
abstract: To assess whether early lung cancer prediction might be informed by an mRNA assay for 5-fluorouracil pathway genes in peripheral blood mononuclear cells (PBMNCs), we examined specimens taken from 51 adenocarcinoma patients and 38 controls (including six patients with benign tumors). PBMNCs and tumor-tissue specimens were taken for measurement of the mRNAs of various 5-fluorouracil pathway genes [thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD), thymidine phosphorylase (TP), and orotate phosphoribosyl transferase (OPRT)]. By quantitative RT-PCR, all four mRNAs were detected in both PBMNCs and tumor tissues. In PBMNCs, TS mRNA/GAPDH mRNA levels were significantly higher in adenocarcinoma patients than in the controls, and significantly higher for pathological stages 2-4 and lymph-node involvement pN1-pN3 than for pathological stage 1 and pN0, respectively. No correlation between PBMNCs and tumor-tissue specimens was found for the level of any mRNA. Thus, the measurement of TS mRNA in PBMNCs might aid the diagnosis of lung adenocarcinoma.
authors.affiliation: Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa 359-8513, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Ogata
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ozeki
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Fujita
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Tanaka
authors.forename: Seiki
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Tadashi
authors.initials: T
authors.lastname: Mio
authors.forename: Hiromi
authors.initials: H
authors.lastname: Wada
authors.forename: Sadayuki
authors.initials: S
authors.lastname: Hiroi
authors.forename: Susumu
authors.initials: S
authors.lastname: Tominaga
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Aida
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kawai
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology*; D000077192:Adenocarcinoma of Lung; D000368:Aged; D000369:Aged, 80 and over; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000235:genetics; D005260:Female; D005472:Fluorouracil / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D009962:Orotate Phosphoribosyltransferase / Q000235:genetics; D010865:Pilot Projects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013939:Thymidine Phosphorylase / Q000235:genetics; D013940:Thymidylate Synthase / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D042943:Dihydrouracil Dehydrogenase (NADP); D013940:Thymidylate Synthase; D009962:Orotate Phosphoribosyltransferase; D013939:Thymidine Phosphorylase; D005472:Fluorouracil