A microplate-based method for real-time monitoring of Saccharomyces cerevisiae viability.
Measuring the viability of the cells after chemical or physical stresses is a routine method in the determination of phenotypes in yeast. Numerous analytical techniques have been applied to the measurement of yeast cell viability, but few of them are suitable for real-time monitoring. Here we propose a microplate-based method for real-time monitoring of Saccharomyces cerevisiae cell viability. This method is also suitable for high-throughput measurement.
Ye
Yan-Rui
YR
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, People's Republic of China.
Ye
Mei-Li
ML
Li
Li-Li
LL
Lin
Ying
Y
D005434:Flow Cytometry / Q000379:methods*; D057166:High-Throughput Screening Assays; D008751:Methylene Blue / Q000737:chemistry; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D013312:Stress, Physiological
D008751:Methylene Blue