[Protective effect of carnosine on the injury of rat vascular endothelial cells induced by hypoxia].
OBJECTIVE
To investigate the effect of camosine on the injury of rat vascular endothelial cells(VECs) induced by hypoxia.


METHODS
The model of the injury of rat VECs induced by hypoxia was established. The effect of camosine on injury of VECs activity induced by hypoxia was determined by MTT assay. The levels of lactate dehydrogenase (LDH) activity in cell medium were measured with corresponding kit. The cell structure was observed under microscope after Coomassie brilliant blue R-250 staining.


RESULTS
After culturing VECs with camosine (10 to 20 mmol/L) for 6 hours, the decrease in VECs activity induced by 12 and 24 hour hypoxia was inhibited. The release of LDH was also inhibited, and the integrity of cell structure remained.


CONCLUSIONS
Camosine has the protective effect on hypoxia injured VECs.
Bai
Jian
J
Department of Biochemistry and Molecular Biology, Liaoning Medical University, Jinzhou 110021, China.
Chi
Ge
G
Zhang
Jun
J
Liu
Li-na
LN
Fan
Miao
M
Liu
Hai-dong
HD
Li
Wei-wei
WW
Yang
Jing
J
D000818:Animals; D002336:Carnosine / Q000494:pharmacology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000473:pathology*; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology*; D008297:Male; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D016166:Free Radical Scavengers; D020011:Protective Agents; D002336:Carnosine