title: [Dimethylsulfoxide-induced expression of thymic stromal lymphopoietin in human bronchial epithelial cells].
abstract: OBJECTIVE
  To investigate the effect of dimethylsulfoxide on the expression of thymic stromal lymphopoietin (TSLP) in human bronchial epithelial cell (HBE).
  
  
  METHODS
  16HBE cells were incubated in the presence of dimethylsulfoxide at different concentrations, and the cell proliferation changes were observed. The expressions of TSLP mRNA and protein in the cells were detected by real-time quantitative PCR and ELISA, respectively.
  
  
  RESULTS
  Dimethylsulfoxide induced significantly increased TSLP mRNA expression in HBE cells (P<0.01) in a concentration-dependent manner. The level of TSLP protein in the supernatant was also increased after dimethylsulfoxide treatment, but high concentration of dimethylsulfoxide resulted in e inhibited cell proliferation.
  
  
  CONCLUSIONS
  Dimethylsulfoxide may affect the immunomodulatory function of HBE cells.
authors.affiliation: Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. winterpower@163.com
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.forename: Shao-xi
authors.initials: SX
authors.lastname: Cai
authors.forename: Hai-jin
authors.initials: HJ
authors.lastname: Zhao
authors.forename: Lai-yu
authors.initials: LY
authors.lastname: Liu
mesh_terms: D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D004121:Dimethyl Sulfoxide; D000094632:Thymic Stromal Lymphopoietin