Correlation of changes in pineal gland cAMP metabolism with light-mediated decrease in N-acetyltransferase activity.
The present study investigated the correlation between the light-mediated decrease in rat pineal N-acetyltransferase (NAT) observed in vivo and changes in cAMP metabolism. While exposing dark-adapted rats to light for short time periods (0-10 min) resulted in a rapid decrease in pineal NAT activity, cAMP exhibited a biphasic response. Following light exposure for 30 s there was a 50% decrease in cAMP levels. However, after 6 min of light exposure the cyclic nucleotide levels had increased 2-3 times above control values. These responses were prevented by phenoxybenzamine pretreatment and the initial decrease was mimicked by i.v. propranolol administration. Examination of cAMP metabolic enzymes, adenylate cyclase and phosphodiesterase revealed an increase in adenylate cyclase activity following 6 min of exposure to light. We discussed how the results observed in vivo compare with those observed using cultured pinealocytes.
Alphs
L
L
Section on Biochemical Pharmacology, Hypertension-Endocrine Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20205, U.S.A.
Miller
L P
LP
Lovenberg
W
W