Protein measurements of microalgal and cyanobacterial biomass.
The protein content of dry biomass of the microalgae Porphyridium cruentum, Scenedesmus almeriensis, and Muriellopsis sp. and of the cyanobacteria Synechocystis aquatilis and Arthrospira platensis was measured by the Lowry method following disruption of the cells by milling with inert ceramic particles. The measurements were compared with the Kjeldahl method and by elemental analysis. The nitrogen-to-protein conversion factors for biomass obtained from exponentially growing cells with a steady state doubling time of approximately 23 h were 5.95 for nitrogen measured by Kjeldahl and 4.44 for total nitrogen measured by elemental analysis. The protein content in dry biomass ranged from 30% to 55%. The above conversion factors are useful for estimating the protein content of microalgal biomass produced in rapid steady state growth as encountered in many commercial production processes.
López
Cynthia Victoria González
CV
Department of Chemical Engineering, University of Almería, E04120 Almería, Spain.
García
María del Carmen Cerón
Mdel C
Fernández
Francisco Gabriel Acién
FG
Bustos
Cristina Segovia
CS
Chisti
Yusuf
Y
Sevilla
José María Fernández
JM
D020418:Algal Proteins / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D018533:Biomass; D002417:Cattle; D000458:Cyanobacteria / Q000378:metabolism*; D009584:Nitrogen / Q000032:analysis; D044665:Porphyridium / Q000378:metabolism*; D012015:Reference Standards; D012710:Serum Albumin, Bovine / Q000378:metabolism
D020418:Algal Proteins; D001426:Bacterial Proteins; D012710:Serum Albumin, Bovine; D009584:Nitrogen