title: Platelet-rich plasma in bone repair of irradiated tibiae of Wistar rats.
abstract: OBJECTIVE
  To evaluate the influence of PRP addition on bone repair of circular defects created in irradiated tibiae of rats by histometric analysis.
  
  
  METHODS
  Sixty male Wistar rats had the right tibiae irradiated with 30 Gy. After 30 days monocortical defects were created and platelet-rich plasma was applied in 30 rats. In the control group defects were created but not filled. The animals were desanguinated after 4, 7, 14, 21, 56 and 84 days and the tibiae removed for histological processing.
  
  
  RESULTS
  There was a tendency in the PRP group to increased bone neoformation from 14-days to 84-days; in the control group increased bone neoformation was not seen after 21 days or later.
  
  
  CONCLUSIONS
  The addition of platelet-rich plasma had a beneficial effect in the initial cellular regeneration period and enhanced bone formation in later periods when compared to control.
authors.affiliation: Department of Otorhinolaringology and Head and Neck Surgery, UNIFESP, São Paulo, Brazil. gumieiro@uol.com.br <gumieiro@uol.com.br>
authors.forename: Emne Hammoud
authors.initials: EH
authors.lastname: Gumieiro
authors.forename: Márcio
authors.initials: M
authors.lastname: Abrahão
authors.forename: Ricardo Schmitutz
authors.initials: RS
authors.lastname: Jahn
authors.forename: Helena
authors.initials: H
authors.lastname: Segretto
authors.forename: Maria Tereza de Seixas
authors.initials: MT
authors.lastname: Alves
authors.forename: Ulf
authors.initials: U
authors.lastname: Nannmark
authors.forename: Gösta
authors.initials: G
authors.lastname: Granström
authors.forename: Luciano Lauria
authors.initials: LL
authors.lastname: Dib
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology* / Q000528:radiation effects; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology*; D008297:Male; D010976:Platelet Count; D053657:Platelet-Rich Plasma; D011833:Radiation Injuries, Experimental; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000528:radiation effects*; D013997:Time Factors
chemical_list: D036341:Intercellular Signaling Peptides and Proteins