title: Actinophytocola timorensis sp. nov. and Actinophytocola corallina sp. nov., isolated from soil.
abstract: Two actinomycete strains, ID05-A0653(T) and ID06-A0464(T), were isolated from soils of West Timor and Lombok island, respectively, in Indonesia. 16S rRNA gene sequence analysis clearly demonstrated that the isolates belonged to the family Pseudonocardiaceae and were closely related to the genus Actinophytocola. Strains ID05-A0653(T) and ID06-A0464(T) exhibited 98.1 and 98.2 % 16S rRNA gene sequence similarity, respectively, with Actinophytocola oryzae GMKU 367(T). The isolates grew well on ISP media and produced white aerial mycelium. Short spore chains were formed directly on the substrate mycelium. The isolates contained meso-diaminopimelic acid, arabinose and galactose as cell-wall components, MK-9(H(4)) as the sole isoprenoid quinone, iso-C(16 : 0) as the major cellular fatty acid and phosphatidylethanolamine as the diagnostic polar lipid. The DNA G+C contents of strains ID05-A0653(T) and ID06-A0464(T) were 69.7 and 71.2 mol%, respectively. On the basis of phenotypic characteristics, DNA-DNA relatedness and 16S rRNA gene sequence comparisons, strains ID05-A0653(T) and ID06-A0464(T) each represent a novel species of the genus Actinophytocola, for which the names Actinophytocola timorensis sp. nov. (type strain ID05-A0653(T)  = BTCC B-673(T)  = NBRC 105524(T)) and Actinophytocola corallina sp. nov. (type strain ID06-A0464(T)  = BTCC B-674(T)  = NBRC 105525(T)) are proposed.
authors.affiliation: NITE Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Misa
authors.initials: M
authors.lastname: Otoguro
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu 400-8511, Japan.|NITE Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamamura
authors.affiliation: NITE Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
authors.affiliation: Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
authors.forename: Rohmatussolihat
authors.initials: R
authors.lastname: Irzaldi
authors.affiliation: Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
authors.forename: Shanti
authors.initials: S
authors.lastname: Ratnakomala
authors.affiliation: Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
authors.forename: Roni
authors.initials: R
authors.lastname: Ridwan
authors.affiliation: Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
authors.forename: Gina
authors.initials: G
authors.lastname: Kartina
authors.affiliation: Research Center for Biology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
authors.forename: Evi
authors.initials: E
authors.lastname: Triana
authors.affiliation: Research Center for Biology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
authors.forename: Arif
authors.initials: A
authors.lastname: Nurkanto
authors.affiliation: Department of Biology, Faculty of Mathematics and Natural Science, Bogor Agricultural University, Jl. Raya pajajaran, Bogor 16144, Indonesia.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Lestari
authors.affiliation: Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
authors.forename: Puspita
authors.initials: P
authors.lastname: Lisdiyanti
authors.affiliation: Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
authors.forename: Yantyati
authors.initials: Y
authors.lastname: Widyastuti
authors.affiliation: NITE Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Ando
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001089:Arabinose / Q000032:analysis; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005690:Galactose / Q000032:analysis; D007214:Indonesia; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000166:cytology
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid; D001089:Arabinose; D005690:Galactose