title: Effect of peroxynitrite scavenging on endothelial cells stimulated by plasma from women with preeclampsia: a proteomic approach.
abstract: OBJECTIVE
  Increased peroxynitrite formation in endothelial cells incubated with plasma from women with preeclampsia alters the expression of specific endothelial proteins.
  
  
  METHODS
  We used a proteomic approach to examine the effect of a peroxynitrite scavenger (FeTPPS, 5 micromol/L) on the expression of endothelial proteins in response to plasma from women with preeclampsia. After treatment, cells were subjected to two-dimensional gel electrophoresis and proteins were identified using mass spectrometry.
  
  
  RESULTS
  Peroxynitrite scavenging decreased the expression of structural proteins, cytokeratin, actin-gamma-1, and moesin, and the calcium-binding protein, calumenin.
  
  
  CONCLUSIONS
  This study has identified novel proteins that are regulated by peroxynitrite in response to plasma from women with preeclampsia.
authors.affiliation: Department of Physiology, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Sowndramalingam
authors.initials: S
authors.lastname: Sankaralingam
authors.forename: Manoj M
authors.initials: MM
authors.lastname: Lalu
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.forename: Sandra T
authors.initials: ST
authors.lastname: Davidge
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D018384:Oxidative Stress; D030421:Peroxynitrous Acid / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D040901:Proteomics; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism*
chemical_list: D030421:Peroxynitrous Acid