Modified porphyrin-brucine conjugated to gold nanoparticles and their application in photodynamic therapy.
Two porphyrin-brucine quaternary ammonium salts were immobilized on gold nanoparticles and their suitability for both in vitro and in vivo photodynamic therapy (PDT) was assayed using the basaloid squamous cell carcinoma PE/CA-PJ34 cell line. In vitro PDT experiments revealed that the gold nanoparticle-bound conjugates were less effective than unbound conjugates in killing cells. However, the same conjugates were more effective in reducing tumor size in vivo, with complete tumor regression observed.
Záruba
Kamil
K
Department of Analytical Chemistry, Faculty of Chemical Engineering, Institute of Chemical Technology Prague, Technická 5, 166 28 Prague 6. kamil.zaruba@vscht.cz
Králová
Jarmila
J
Rezanka
Pavel
P
Poucková
Pavla
P
Veverková
Lenka
L
Král
Vladimír
V
D000478:Alkylation; D000818:Animals; D001692:Biological Transport; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy; D010778:Photochemotherapy / Q000379:methods*; D011166:Porphyrins / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013331:Strychnine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use
D011166:Porphyrins; D012997:Solvents; C083806:brucine; D006046:Gold; D013331:Strychnine