Modified siRNAs for the study of the PAZ domain.
Chemical modifications aimed at stabilizing the interaction between the 3'-end of siRNAs and the PAZ domain of RISC have been tested for their effect on RNAi activity. Such modifications contribute positively to the stability of siRNAs in human serum.
Somoza
Alvaro
A
IMDEA-Nanociencia, 28049, Madrid, Spain. alvaro.somoza@imdea.org
Terrazas
Montserrat
M
Eritja
Ramón
R
D045744:Cell Line, Tumor; D006367:HeLa Cells; D006801:Humans; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry*; D034743:RNA-Induced Silencing Complex / Q000737:chemistry*
D034741:RNA, Small Interfering; D034743:RNA-Induced Silencing Complex