title: Validation study of a rapid ELISA for detection of aflatoxin in corn. Performance Tested Method 050901.
abstract: Neogen Corp. developed the Veratox aflatoxin test kit for the detection of total aflatoxin. The purpose of this study was to validate the method under the requirements of the AOAC Research Institute Performance Tested Methods (PTM) program. There are several AOAC Official Methods for total aflatoxin detection in corn (994.08, 990.33, 979.18, 993.17, 990.32, 993.16, 991.31, and 990.74), varying between rapid and analytical-based methods and one rapid method that has been performance tested by the AOAC Research Institute (PTM 030701). However, the widely used reference method is AOAC Official Method 994.08, which is an HPLC method and is referred to as the reference method in this paper. Although considered the reference method, the HPLC procedure is complicated and requires the investment of both expensive equipment and a highly skilled technician. A rapid (e.g., ELISA) test kit to be validated by the AOAC Research Institute is needed.
authors.affiliation: Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA. tlupo@neogen.com
authors.forename: Anthony
authors.initials: A
authors.lastname: Lupo
authors.forename: Chris
authors.initials: C
authors.lastname: Roebuck
authors.forename: Monica
authors.initials: M
authors.lastname: Dutcher
authors.forename: Justina
authors.initials: J
authors.lastname: Kennedy
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abouzied
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D000383:Agriculture; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D009183:Mycotoxins / Q000378:metabolism; D015203:Reproducibility of Results; D013997:Time Factors; D003313:Zea mays / Q000378:metabolism*
chemical_list: D000348:Aflatoxins; D009183:Mycotoxins