[Simultaneous detection of measles and rubella virus by multiplex real-time RT-PCR with an internal control].
Measles and rubella virus cause fever rash diseases that are uneasy to differentiate clinically from each other. Specific primers and fluorescence-labeled probes were designed, and a multiplex Real-time RT-PCR with an internal control was developed to simultaneously identify the measles and rubella virus. The multiplex Real-time RT-PCR assay was specific and no false positive or false negative results were found. The sensitivity of the assay was 0.1TCID50/mL and 1TCID50/mL for measles and rubella virus respectively. Analysis with 0.1-10(3)TCID50/mL measles or rubella virus samples demonstrated high validity and reproducibility with the coefficient of variability(CV) of below 0.9% for both measles and rubella virus. Using ribonuclease P (RNase P) as internal false negative control, the developed multiplex Real-time RT-PCR assay is suitable for rapid clinical diagnosis of measles and rubella virus.
Yu
Bei-Bei
BB
Department of Medical Microbiology and Parasitology, Medical school, Zhejiang University, Hangzhou 310058, China.
Feng
Yan
Y
Xu
Chang-Ping
CP
Lu
Yi-Yu
YY
Qian
Jing
J
D005456:Fluorescent Dyes; D006801:Humans; D008457:Measles / Q000175:diagnosis / Q000821:virology; D008459:Measles virus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D043262:Ribonuclease P / Q000235:genetics; D012409:Rubella / Q000175:diagnosis / Q000821:virology; D012412:Rubella virus / Q000235:genetics*; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D012367:RNA, Viral; D043262:Ribonuclease P