[Killing effect of cytotoxicty T lymphocyte primed by two different methods preparation of dendritic cells pulsed with antigen on Tca8113 cells in vitro].
OBJECTIVE
To study the feasibility of dendritic cells (DCs) vaccine on the therapy of tongue carcinoma and find the better way of antigen load.


METHODS
The antigen peptides of Tca8113 cells were obtained by acid eluted technique and repetitive freeze thaw method. Separating T cell and inducing dendritic cells were obtained from human peripheral blood monocyte. Divided into three groups: Weak acid elution method antigen group, anti-freeze-thaw method antigen group, and the control group (without tumor antigen). T cells and UCs were mixed to culture by different effector-target ratio. Using MTT assay measured the quantities of absorbance and calculated stimulation index. Dendrtic cells pulsed with antigen were mixed with T cells by different effector-target ratio. MIT assay was used to measure the quantities of absorbance and calculate killing rate.


RESULTS
DCs vaccine was constructed successfully. DCs vaccine can induce T lymphocytes to kill Tca8113 cells and display the dose-effect relationship. There was significant difference among the three groups. The acid eluted and repetitive freeze thaw groups were better than the control group. The acid eluted group was better than repetitive freeze thaw group.


CONCLUSIONS
DCs vaccine can induce T lymphocytes to kill Tca8113 cells. The antigen peptides obtained by acid eluted technique is better than repetitive freeze thaw method in immunotherapy of tongue cancer.
Zhi
Ke-qian
KQ
Dept. of Oral and Maxillofacial Surgery, Stomatological Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
Xu
Yan
Y
Ren
Wen-hao
WH
Gao
Ling
L
Zhao
Lu
L
Yang
Yong
Y
Zhang
Yin-cheng
YC
D003713:Dendritic Cells; D006801:Humans; D066298:In Vitro Techniques; D013602:T-Lymphocytes, Cytotoxic; D014062:Tongue Neoplasms