title: [Methods of establishing human immortalized cell lines].
abstract: The development of cancer is a multistages process that involving widely varies genes. The oncogenes and anti-oncogenes play an important role during this process. However, little is known about the molecular mechanisms responsible for the development. We cann't understand the process of cancer in human bodies actually,so we should rely on the use of the human cells in culture. While the majority of carcinogenesis studies have relied on the use of rodent cells in culture, experimental models to define the role of carcinogenic agents in the development of cancer must be established by using a variety of human cells.Therefore,a variety of human cell lines need to be established. Supported by Research Fund of Science and Technology Commission of Shanghai Municipality(Grant No.08JC1412900) and Shanghai Leading Academic Discipline Project (Grant No.S30206-kf05).
authors.affiliation: Department of Stomatology, First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China. wangtingle_andy@hotmail.com
authors.forename: Ting-le
authors.initials: TL
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Song
mesh_terms: D018929:Cell Culture Techniques; D002460:Cell Line; D006801:Humans