Metabolomics in serum of ovariectomised rats and those exposed to 17β-oestradiol and genistein.
OBJECTIVE
Metabolites profiling provides global insights in characterisations and interactions of metabolites under specific physiological or pathologic states. The aim of this study was to examine serum metabolic changes in bilateral ovariectomised (OVX) rats and those exposed to 17β-oestradiol (E(2), 30 μg/kg bw, sc.) or genistein (50 μg/kg bw, sc.) for 12 weeks.


METHODS
Serum samples were detected by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-q-TOF-MS). Significant variables were found by the orthogonal partial least-squares analysis and identified by matching their tandem MS fragmentation patterns with reference standards.


RESULTS
There were 54 variables changed in OVX group compared with sham group. Genistein might induce more intensive changes of metabolites than E(2) in OVX rats. Among the 54 significant variables of OVX group, 8 and 20 were found to be affected by E(2) and genistein, respectively. Finally, four of them were identified as arachidonic acid (AA), eicosapentaenoic acid (EPA), ergocalciferol and cholecalciferol. Both E(2) and genistein could reverse levels of AA, EPA and ergocalciferol to sham levels. Only genistein treatment could reverse the level of cholecalciferol.


CONCLUSIONS
This study provided a serum profile of ovary dysfunction and showed some similarities and differences between E(2) and genistein treatments of the OVX model.
Zhu
Xiaoyan
X
Department of Physiology, School of Basic Medical Science, Second Military Medical University, Shanghai 200433, China.
Liu
Xinru
X
He
Ping
P
Cao
Buqing
B
Lv
Yonghai
Y
Zhang
Weidong
W
Ni
Xin
X
D000818:Animals; D015415:Biomarkers / Q000097:blood; D002853:Chromatography, Liquid; D004958:Estradiol; D005260:Female; D019833:Genistein; D013058:Mass Spectrometry; D055442:Metabolome; D055432:Metabolomics; D015999:Multivariate Analysis; D010052:Ovariectomy; D017698:Postmenopause / Q000097:blood*; D051381:Rats
D015415:Biomarkers; D004958:Estradiol; D019833:Genistein