Effect of nonfat dry milk and major whey components on interleukin-6 and interleukin-8 production in human intestinal epithelial-like Caco-2 cells.
Bovine nonfat dry milk (NDM) and major whey components (lactose, alpha-lactalbumin, and beta-lactoglobulin) were evaluated for their effects on IL-6 and IL-8 production in human intestinal-like Caco-2 cells unstimulated or stimulated with IL-1beta. All the whey components investigated and NDM induced IL-6 production by Caco-2 cells; the most significant increase was observed with beta-lactoglobulin. In the case of IL-1beta-stimulated cells, neither NDM nor the major whey components investigated contributed to the induction of IL-6 production after they were stimulated. Induction of IL-8 production by both alpha-lactalbumin and beta-lactoglobulin was higher than that by lactose and NDM; alpha-lactalbumin was a more potent inducer of IL-8 than beta-lactoglobulin and IL-1beta alone in both unstimulated and stimulated cells. In Caco-2 cells that were stimulated with IL1-beta, NDM and all the major whey components investigated had a synergistic effect on induction of IL-8 production, indicating that IL-8 induction was amplified by prior stimulation of cells by IL-1beta. This synergistic effect was not observed with IL-6. Our results suggest that immunomodulatory properties of milk components may be affected by other complex events in the gut.
Ustunol
Z
Z
Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824-1224, USA. ustunol@msu.edu
Wong
C
C
D000818:Animals; D018938:Caco-2 Cells / Q000187:drug effects / Q000378:metabolism*; D002417:Cattle; D003611:Dairy Products; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D015850:Interleukin-6 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D007768:Lactalbumin / Q000494:pharmacology; D007782:Lactoglobulins / Q000494:pharmacology; D007785:Lactose / Q000494:pharmacology; D008892:Milk / Q000378:metabolism*
D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D007782:Lactoglobulins; D007768:Lactalbumin; D007785:Lactose