Central regulation of gastric acetylcholine metabolism and acid output Analysis using stress and 2-deoxy-d-glucose administration in rats.
The stress of immobilization in water caused a significant increase in the activity of choline acetyltransferase (CAT) and acetylcholinesterase (AChE), acetylcholine (ACh) content in the stomach and gastric acid secretion, but a decrease of choline content in rats. The increase in CAT activity began 1 h after the application of stress, peaked in 3 h and gradually decreased to normal within 7 h. Similar alterations in gastric acid secretion were observed. The ACh content in stomach tissue increased 30 min after the application of stress and remained elevated for 2.5 h. The content decreased to control levels after 5 h, and significantly increased again after 7 h. The choline content in stomach tissue significantly decreased 1 and 2 h after stress but returned to normal 3 h after the application. An increase in AChE activity was observed 2 and 7 h after the application of stress but normal levels were found after 4 h. Increases in CAT activity and acid output were also observed following administration of 2-deoxy-d-glucose (2-DG), but no changes in ACh and choline contents or AChE activity were observed. The increases in CAT activity and the acid secretion caused by stress and 2-DG administration were blocked by administration of hexamethonium. These results suggest that increases in gastric CAT, AChE activities and ACh content and a decrease of choline content in the early stages are results of increased vagus nerve activity, which influences gastric acid secretion. Furthermore, they suggest that alterations in ACh content and AChE activity at a later stage are less directly related to the increase in vagus nerve activity.
Muramatsu
M
M
Research Center, Taisho Pharmaceutical Co. Ltd, Ohmiya, Saitama 330, Japan.
Arai
I
I
Tamaki
J
J
Hirose
H
H
Fujita
A
A
Aihara
H
H