title: DNA analysis of ancient skeletal remains.
abstract: Non-Governmental Organization Archaia (http://www.archaia.cz) carried out the rescue archaeological research at Knezeves near Prague in 1998. Most of dating objects in Knezeves come from the period of Late and Final Bronze Age. The approximately 3,000 years old set, which included 11 human remains from three settlement features, was collected for the study. First, gender was determined according to anthropological characteristics. Ancient DNA from bones was extracted by the phenol-chloroform procedure and N-phenacetylthiazolum bromide reagent. Polymerase chain reaction amplification of AMEL XY, part of amelogenin gene, with subsequent polyacrylamide gel electrophoresis and Short Tandem Repeats analysis followed. DNA profiles of skeletal remains were obtained by the fragmentation analysis of autosomal short tandem repeat markers. Genetic profiles showed us whether individuals from Knezeves were in mutual relationship (parent - descendant). The congruence of results in sex determination supported reliability of genetic methods, which are suitable for sex determination of fragmental and subadult skeletal remains.
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Anthropology and Human Genetics, Prague, Czech Republic. i.eliasova@seznam.cz
authors.forename: I
authors.initials: I
authors.lastname: Eliásová
authors.forename: I
authors.initials: I
authors.lastname: Mazura
authors.forename: L
authors.initials: L
authors.lastname: Smejtek
mesh_terms: D001106:Archaeology / Q000379:methods*; D001483:Base Sequence; D001842:Bone and Bones / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D005260:Female; D049690:History, Ancient; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA