title: Labeling of phosphoinositides in rat brain membranes: an assessment of changes due to post-decapitative ischemic treatment.
abstract: Metabolic changes in brain phosphoinositides with respect to post-decapitative ischemic treatment were examined with rats labeled after i.p. injection of (32)Pi and intracerebral injection of [(3)H]inositol. The ischemic treatment resulted in a large and rapid decrease (40% in 2 min) in labeled polyphosphoinositide (poly-Pl), regardless of the source of the labeling. The rapid disappearance of poly-PI labeling can be similarly detected in the synaptosomes and plasma membrane fractions. On the other hand, the ischemic treatment resulted in an increase (10%) in [(32)P]-labeling of phosphatidylinositol, indicating possible contribution due to the poly-PI phosphomonoesterase pathway. In addition to the decrease in labeling of poly-PI, there was a decrease in radioactivity of phosphatidic acids in brain homogenates and plasma membranes due to the ischemic treatment. The labeling pattern of other phospholipids was not altered by the ischemic treatment. With rats prelabeled with [(3)H]inositol, the amount of labeled inositol monophosphate in brain increased 4-fold after pretreatment with LiCl (8 meq/kg). While no obvious change in labeling of inositol bisphosphate and inositol monophosphate was observed, there was a 40% decrease in labeled inositol trisphosphate after 2 min ischemic treatment. Discussions were made regarding the advantage and disadvantages in labeling brain phosphoinositides with these two types of labeled precursors.
authors.affiliation: Biochemistry Department and Sinclair Comparative Medicine Research Farm, University of Missouri, Columbia, MO 65203, U.S.A.
authors.forename: G Y
authors.initials: GY
authors.lastname: Sun
authors.forename: S F
authors.initials: SF
authors.lastname: Huang