Improvement of amperometric glucose biosensor by the immobilization of FcCD inclusive complex and carbon nanotube.
A novel glucose biosensor was constructed by using ferrocene-carbonyl-beta-cyclodextrin (FcCD) inclusive complex as electron-transfer mediator and carbon nanotubes (CNTs) as electron-transfer promoter. FcCD inclusive complex and glucose oxidase (GOx) were covalently bonded to CNTs by poly-l-lysine (PLL) to fabricate a glucose biosensor. The electrocatalytic oxidation of glucose at the biosensor occurred at low potential below 200 mV, avoiding the interference of the main interfering substances in real samples containing a 5-times higher concentration of l-cysteine, ascorbic acid, and uric acid. The biosensor showed fast response for glucose. A broad linear range of glucose concentration from 1.0 x 10(-5) to 2.90 x 10(-3) M was obtained and the detection limit was 2.2 x 10(-6) M.
Zheng
Longzhen
L
Department of Chemistry and Chemical Engineering, East China Jiao Tong University, Nanchang, Jiangxi 330013, PR China. zlz@ecjtu.jx.cn
Li
Junhua
J
Xu
Jingpeng
J
Xiong
Leyan
L
Zheng
Dan
D
Liu
Qiang
Q
Liu
Wen
W
Li
Yindi
Y
Yang
Shaoming
S
Xia
Jian
J
D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D005296:Ferrous Compounds / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D000075163:Metallocenes; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D011107:Polylysine / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D005296:Ferrous Compounds; D000075163:Metallocenes; D037742:Nanotubes, Carbon; D047392:beta-Cyclodextrins; D011107:Polylysine; D005949:Glucose Oxidase; D005947:Glucose; C004998:ferrocene