Solid-phase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals.
The aim of this work was to develop a method for the clean-up of a mycotoxin, i.e. Ochratoxin A (OTA), from cereal extracts employing a new molecularly imprinted polymer (MIP) as selective sorbent for solid-phase extraction (SPE) and to compare with an immunoaffinity column. A first series of experiments was carried out in pure solvents to estimate the potential of the imprinted sorbent in terms of selectivity studying the retention of OTA on the MIP and on a non-imprinted polymer using conventional crushed monolith. The selectivity of the MIP was also checked by its application to wheat extracts. Then, after this feasibility study, two different formats of MIP: crushed monolith and micro-beads were evaluated and compared. Therefore an optimization procedure was applied to the selective extraction from wheat using the MIP beads. The whole procedure was validated by applying it to wheat extract spiked by OTA at different concentration levels and then to a certified contaminated wheat sample. Recoveries close to 100% were obtained. The high selectivity brought by the MIP was compared to the selectivity by an immunoaffinity cartridge for the clean-up of the same wheat sample. The study of capacity of both showed a significant higher capacity of the MIP.
Ali
Wassim Hadj
WH
Department of Environmental and Analytical Chemistry (UMR PECSA 7195 CNRS-UPMC Paris 06-ESPCI ParisTech), ESPCI ParisTech, 10 rue Vauquelin, 75231 Paris cedex 05, France.
Derrien
Delphine
D
Alix
Florent
F
Pérollier
Céline
C
Lépine
Olivier
O
Bayoudh
Sami
S
Chapuis-Hugon
Florence
F
Pichon
Valérie
V
D000327:Adsorption; D002846:Chromatography, Affinity / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D007163:Immunosorbent Techniques; D054802:Molecular Imprinting; D009793:Ochratoxins / Q000302:isolation & purification*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D014908:Triticum / Q000737:chemistry*
D009793:Ochratoxins; C025589:ochratoxin A