[Influence of Chrysanthemum indium on collagen accumulation and signaling transduction pathways in left ventricular tissue of cardiac hypertrophy in rats].
OBJECTIVE
To evaluate the influence of Chrysanthemum indium on collagen accumulation and signaling transduction pathways in left ventricle tissue of cardiac hypertrophy induced by abdominal aortic banding in rats.


METHODS
Ventricular remodeling was induced by abdominal aortic banding (AAB) in rats. After 35 day treatment, the blood pressure was measured, then the ratios of LVW/BW and HW/BW were calculated. The histological assay was performed by HE staining for determining the myocardium cell cross section and picric acid/sirius red staining for determining collagen content. Immunohistochemistry was used to detect the protein expressions of PKC, bFGF and P38.


RESULTS
The experimental data demonstrated that C. indium could decrease blood pressure and the cardiac indexes of LVW/BW and HW/BW, significantly diminish cross sectional area of cardiomyocyte, ameliorate collagen accumulation such as collagen volume fraction, perivascular collagen area and collagen distributions of type I and II and significantly down regulate the protein expressions of PKC, bFGF and P38 (P<0.05).


CONCLUSIONS
C. indium can significantly attenuate the experimental ventricular remodeling. The mechanism may be related to reducing the blood pressure, decreasing the total collagen content of left ventricle tissue and modulating signaling transduction pathway.
Wu
Qi
Q
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Chen
Changxun
C
Gu
Weiliang
W
Gao
Jianping
J
Liu
Ying
Y
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D006332:Cardiomegaly / Q000188:drug therapy* / Q000378:metabolism; D031202:Chrysanthemum; D003094:Collagen / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000032:analysis; D006352:Heart Ventricles / Q000378:metabolism*; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011493:Protein Kinase C / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects*; D020257:Ventricular Remodeling / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000032:analysis
D010936:Plant Extracts; D016222:Fibroblast Growth Factor 2; D003094:Collagen; D011493:Protein Kinase C; D048051:p38 Mitogen-Activated Protein Kinases