Differential protection of pre-, co- and post-treatment of curcumin against hydrogen peroxide in PC12 cells.
Pharmacological potential of curcumin was assessed in PC12 cells against hydrogen peroxide (H(2) O(2)) exposure. In MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and lactate dehydrogenase (LDH) assays, 24-hour exposure of H(2)O(2) (0.5 mM and above) was found to be cytotoxic. A significant (p < 0.001) increase in percentage cell viability was recorded in PC12 cells pretreated with curcumin (25, 50 and 100 µg/mL) for 24 hours prior to H(2)O(2) (0.5 and 1 mM) exposure for 24 hours. Co-exposure to H(2)O(2) and curcumin was also found effective. However, a therapeutic treatment of curcumin for 24 hours after H(2)O(2) exposure to the cells was found ineffective. Differential response of PC12-H(2)O(2) model to curcumin in MTT and LDH assays suggests the utility of these endpoints to sort the drug candidates to study their antioxidant potential.
Siddiqui
M A
MA
Indian Institute of Toxicology Research, Lucknow, India (Council of Scientific & Industrial Research, New Delhi, India.
Kashyap
M P
MP
Kumar
V
V
Tripathi
V K
VK
Khanna
V K
VK
Yadav
S
S
Pant
A B
AB
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000633:toxicity*; D016877:Oxidants / Q000633:toxicity*; D016716:PC12 Cells; D051381:Rats; D013997:Time Factors
D000975:Antioxidants; D016877:Oxidants; D006861:Hydrogen Peroxide; D003474:Curcumin