Mechanical non-surgical treatment of peri-implantitis: a single-blinded randomized longitudinal clinical study. II. Microbiological results.
BACKGROUND
Peri-implantitis is common in patients with dental implants. We performed a single-blinded longitudinal randomized study to assess the effects of mechanical debridement on the peri-implant microbiota in peri-implantitis lesions.


METHODS
An expanded checkerboard DNA-DNA hybridization assay encompassing 79 different microorganisms was used to study bacterial counts before and during 6 months following mechanical treatment of peri-implantitis in 17 cases treated with curettes and 14 cases treated with an ultrasonic device. Statistics included non-parametric tests and GLM multivariate analysis with p<0001 indicating significance and 80% power.


RESULTS
At selected implant test sites, the most prevalent bacteria were: Fusobacterium nucleatum sp., Staphylococci sp., Aggregatibacter actinomycetemcomitans, Helicobacter pylori, and Tannerella forsythia. 30 min. after treatment with curettes, A. actinomycetemcomitans (serotype a), Lactobacillus acidophilus, Streptococcus anginosus, and Veillonella parvula were found at lower counts (p<0.001). No such differences were found for implants treated with the ultrasonic device. Inconsistent changes occurred following the first week. No microbiological differences between baseline and 6-month samples were found for any species or between treatment study methods in peri-implantitis.


CONCLUSIONS
Both methods failed to eliminate or reduce bacterial counts in peri-implantitis. No group differences were found in the ability to reduce the microbiota in peri-implantitis.
Persson
G Rutger
GR
Department of Periodontology, University of Bern, Bern, Switzerland. rutger.persson@zmk.unibe.ch
Samuelsson
Emelie
E
Lindahl
Christel
C
Renvert
Stefan
S
D016976:Aggregatibacter actinomycetemcomitans / Q000254:growth & development; D001419:Bacteria / Q000145:classification / Q000254:growth & development*; D001439:Bacteroides / Q000254:growth & development; D002206:Capnocytophaga / Q000254:growth & development; D015169:Colony Count, Microbial; D015921:Dental Implants / Q000382:microbiology*; D003777:Dental Prophylaxis / Q000379:methods*; D005260:Female; D005500:Follow-Up Studies; D016967:Fusobacterium nucleatum / Q000254:growth & development; D016480:Helicobacter pylori / Q000254:growth & development; D006801:Humans; D007778:Lactobacillus / Q000254:growth & development; D007779:Lactobacillus acidophilus / Q000254:growth & development; D008137:Longitudinal Studies; D008297:Male; D010514:Periodontal Pocket / Q000382:microbiology / Q000628:therapy; D010518:Periodontitis / Q000382:microbiology* / Q000628:therapy; D016037:Single-Blind Method; D012907:Smoking; D013210:Staphylococcus / Q000254:growth & development; D013291:Streptococcus / Q000254:growth & development; D034366:Streptococcus anginosus / Q000254:growth & development; D054773:Streptococcus gordonii / Q000254:growth & development; D013295:Streptococcus mutans / Q000254:growth & development; D019533:Streptococcus oralis / Q000254:growth & development; D016896:Treatment Outcome; D014464:Ultrasonic Therapy / Q000379:methods*; D014678:Veillonella / Q000254:growth & development
D015921:Dental Implants