High affinity binding site for (+) amphetamine in rat hypothalamus: Fact or artefact?
We studied the binding of (+)(3)H-amphetamine to membrane sites from rat hypothalamus. Our experiments demonstrate the possible existence of artefactual results in previous studies due to the inadequate use of a filtration technique. In addition, it seems that monoamine oxidase A might be a component of the complex mixture of binding sites which are recognized by (+)(3)H-amphetamine since harmaline and monoamine oxidase A selective inhibitors are good displacers of this ligand.
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Centre de Recherche Delalande, 10 rue des Carrières, F-92500 Rueil-Malmaison, France.
Strolin Benedetti
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Rumigny
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