Evidence for sialic acid on the nicotinic acetylcholine receptor from Torpedo marmorata.
The nicotinic acetylcholine receptor from Torpedo marmorata was treated with neuraminidase. Direct determination of sialic acid released gave about 1 mole sialic acid per mole receptor. Lectin binding studies of the sugars accessible on the receptor molecule were performed after sialic acid hydrolysis. They indicated that the terminal sialic acid is linked to a galactose residue. The present findings confirm the presence of one terminal sialic acid residue per receptor molecule.
Bersinger
N A
NA
Department of Biochemistry, University of Geneva, Sciences II, 1211 Geneva 4, Switzerland.
Velazco
M I
MI
James
R W
RW
Fulpius
B W
BW