A novel, low-volume method for organ culture of embryonic kidneys that allows development of cortico-medullary anatomical organization.
Here, we present a novel method for culturing kidneys in low volumes of medium that offers more organotypic development compared to conventional methods. Organ culture is a powerful technique for studying renal development. It recapitulates many aspects of early development very well, but the established techniques have some disadvantages: in particular, they require relatively large volumes (1-3 mls) of culture medium, which can make high-throughput screens expensive, they require porous (filter) substrates which are difficult to modify chemically, and the organs produced do not achieve good cortico-medullary zonation. Here, we present a technique of growing kidney rudiments in very low volumes of medium-around 85 microliters-using silicone chambers. In this system, kidneys grow directly on glass, grow larger than in conventional culture and develop a clear anatomical cortico-medullary zonation with extended loops of Henle.
Sebinger
David D R
DD
Max Bergmann Center of Biomaterials, Leibniz-Institut für Polymerforschung Dresden, Dresden, Germany.
Unbekandt
Mathieu
M
Ganeva
Veronika V
VV
Ofenbauer
Andreas
A
Werner
Carsten
C
Davies
Jamie A
JA
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000033:anatomy & histology* / Q000187:drug effects; D007672:Kidney Cortex / Q000033:anatomy & histology* / Q000187:drug effects / Q000196:embryology* / Q000254:growth & development; D007679:Kidney Medulla / Q000033:anatomy & histology* / Q000187:drug effects / Q000196:embryology* / Q000254:growth & development; D051379:Mice; D009024:Morphogenesis / Q000187:drug effects; D009399:Nephrons / Q000166:cytology / Q000187:drug effects / Q000196:embryology; D009924:Organ Culture Techniques / Q000379:methods*; D012828:Silicones; D013312:Stress, Physiological / Q000187:drug effects; D013500:Surface Tension / Q000187:drug effects
D003470:Culture Media; D012828:Silicones