[In vitro study on physical and chemical properities of calcium phosphate cement/amifostine complex and on vitality of cultured cells].
This study was designed to assess the feasibility of calcium phosphate cement/amifostine complex as a new material for filling the bone defect caused by tumor resection. Mixed-molding method was used, the mass ratios of 0%, 0.1%, 0.5%, 1%, 2% of amifostine/calcium phosphate cement complex being adopted. The curing time, mechanical strength, porosity, scanning electron micrograph, osteosarcoma cells' vitality and vascular endothelial cells' vitality relevant to the complex in vitro were observed. Calcium phosphate cement being loaded with 0.1% and 0.5% amifostine did not affect the curing time, strength, pore size and porosity of calcium phosphate bone cement. In addition proliferation and differentiation of osteosarcoma cells and vascular endothelial cells were not affected. These data suggest that phosphate cement containing 0.1% and 0.5% amifostine be of significance in the treatment regimen as bone defect filling materials..
Liu
Yanning
Y
Department of Orthopaedics,the First Hospital of Xi 'an Jiantong University, Xi 'an 710061, China. liuyanninglyn@yahoo.com.cn
Liu
Miao
M
Liu
Juan
J
Ren
Pengyu
P
D004999:Amifostine / Q000494:pharmacology*; D001843:Bone Cements / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D055598:Chemical Phenomena; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology
D001843:Bone Cements; D002130:Calcium Phosphates; C020243:calcium phosphate; D004999:Amifostine