Identification of P[8]b subtype in OP354-like human rotavirus strains by a modified RT-PCR method.
In our previous study, a novel P[8] subtype, i.e., P[8]b was identified for human rotavirus strains MMC38 and MMC71 detected in Bangladesh, of which the P types could not be determined by conventional RT-PCR genotyping methods. In the present study, a modified multiplex RT-PCR method was developed to detect P[8]b as well as common human rotavirus P types. With this method, P[8]b was detected in three strains among the 26 rotavirus specimens which had been judged as mixed P types in the previous study in Bangladesh. The VP4 nucleotide sequences of these strains showed more than 98.9% identities to those of strains MMC38 and MMC71. The newly designed RT-PCR method was considered as useful for identifying P[8]b and avoiding misclassification by the conventional RT-PCR genotyping methods.
Nagashima
Shigeo
S
Department of Hygiene, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo, Japan. shigeon@sapmed.ac.jp
Kobayashi
Nobumichi
N
Paul
Shyamal Kumar
SK
Ghosh
Souvik
S
Chawla-Sarkar
Mamta
M
Hossain
Mohammad Akram
MA
Krishnan
Triveni
T
D036022:Capsid Proteins / Q000235:genetics; D006801:Humans; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000821:virology*; D012703:Serotyping
D036022:Capsid Proteins; D012367:RNA, Viral; C060339:VP4 protein, Rotavirus