Mannose production from fructose by free and immobilized D-lyxose isomerases from Providencia stuartii.
A recombinant D-lyxose isomerase from Providencia stuartii was immobilized on Duolite A568 beads which gave the highest conversion of D-fructose to D-mannose among the various immobilization beads evaluated. Maximum activities of both the free and immobilized enzymes for fructose isomerization were at pH 7.5 and 45 degrees C in the presence of 1 mM Mn(2+). Enzyme half-lives were 14 and 30 h at 35 degrees C and 3.4 and 5.1 h at 45 degrees C, respectively. The immobilized enzyme in 300 g fructose/l (replaced hourly), produced 75 g mannose/l at 35 degrees C = 25% (w/w) yield with a productivity of 75 g mannose l(-1) h(-1) after 23 cycles.
Park
Chang-Su
CS
Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, South Korea.
Kwon
Hyun-Jung
HJ
Yeom
Soo-Jin
SJ
Oh
Deok-Kun
DK
D019747:Aldose-Ketose Isomerases / Q000302:isolation & purification / Q000378:metabolism*; D003067:Coenzymes / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D005632:Fructose / Q000378:metabolism*; D006207:Half-Life; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008345:Manganese / Q000378:metabolism; D008358:Mannose / Q000378:metabolism*; D008863:Microspheres; D011532:Providencia / Q000201:enzymology*; D013696:Temperature
D003067:Coenzymes; D004800:Enzymes, Immobilized; D005632:Fructose; D008345:Manganese; D019747:Aldose-Ketose Isomerases; C021525:D-lyxose ketol-isomerase; D008358:Mannose