title: The archaeal exosome localizes to the membrane.
abstract: We studied the cellular localization of the archaeal exosome, an RNA-processing protein complex containing orthologs of the eukaryotic proteins Rrp41, Rrp42, Rrp4 and Csl4, and an archaea-specific subunit annotated as DnaG. Fractionation of cell-free extracts of Sulfolobus solfataricus in sucrose density gradients revealed that DnaG and the active-site comprising subunit Rrp41 are enriched together with surface layer proteins in a yellow colored ring, implicating that the exosome is membrane-bound. In accordance with this assumption, DnaG and Rrp41 were detected at the periphery of the cell by immunofluorescence microscopy. Our finding suggests that RNA processing in Archaea is spatially organized.
authors.affiliation: Institut für Mikrobiologie und Molekularbiologie der JLU Giessen, Giessen, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Roppelt
authors.forename: Cédric F V
authors.initials: CF
authors.lastname: Hobel
authors.forename: Sonja V
authors.initials: SV
authors.lastname: Albers
authors.forename: Christian
authors.initials: C
authors.lastname: Lassek
authors.forename: Heinz
authors.initials: H
authors.lastname: Schwarz
authors.forename: Gabriele
authors.initials: G
authors.lastname: Klug
authors.forename: Elena
authors.initials: E
authors.lastname: Evguenieva-Hackenberg
mesh_terms: D001105:Archaea / Q000378:metabolism*; D019843:Archaeal Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism*; D055354:Exosomes / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D048229:Sulfolobus solfataricus / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins