title: Immunomodulatory activities and subacute toxicity of a novel β-glucan from Paenibacillus polymyxa JB115 in rats.
abstract: Subacute toxicity and immunopharmacological activities of β-glucan from P. polymyxa JB115 was evaluated in a 28-day feeding study in rats. The white blood cell count, red blood cell count, hematocrit, hemoglobin, thrombocytes (THR) and thrombocytocrit were significantly higher in male fed with β-glucan than control rats and the insignificant lower eosinophil count, mean corpuscular volume, mean cell hemoglobin and uninfected THR (uTHR) levels were observed in male whereas no marked changes in female rats. No other significant differences in serum chemistry and liver, kidney, and spleen weights were observed. The pathological changes and other abnormal indicators were not detected in urine. Female rats fed with diet supplemented with 0.01% β-glucan also showed marked increase in the percentage of blood cytotoxic T-lymphocytes compared to that of the control group while not significant differences in the percentage of blood B-lymphocytes. No adverse effects on general condition and behavior, growth, feed and water consumption and feed conversion efficiency were found. The results suggest that consumption of the novel β-1, 3/1, 6-glucan from P. polymyxa JB115 was not associated with any obvious toxic effects in rats, indicating its safety as a potential immunostimulant or as an adjuvant of some animal vaccines.
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Chang
authors.forename: Md Ahsanur
authors.initials: MA
authors.lastname: Reza
authors.forename: Joong-Su
authors.initials: JS
authors.lastname: Lee
authors.forename: Elias
authors.initials: E
authors.lastname: Gebru
authors.forename: Seung-Hee
authors.initials: SH
authors.lastname: Jang
authors.forename: Myung-Jin
authors.initials: MJ
authors.lastname: Choi
authors.forename: Seung-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Dereje
authors.initials: D
authors.lastname: Damte
authors.forename: Jong-Choon
authors.initials: JC
authors.lastname: Kim
authors.forename: Seung-Chun
authors.initials: SC
authors.lastname: Park
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005936:Glucans / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity*; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity*; D008297:Male; D056507:Paenibacillus / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D036401:Toxicity Tests, Chronic
chemical_list: D005936:Glucans; D007155:Immunologic Factors; C033671:epiglucan