Chemical aminoacylation of tRNAs with fluorinated amino acids for in vitro protein mutagenesis.
This article describes the chemical aminoacylation of the yeast phenylalanine suppressor tRNA with a series of amino acids bearing fluorinated side chains via the hybrid dinucleotide pdCpA and ligation to the corresponding truncated tRNA species. Aminoacyl-tRNAs can be used to synthesize biologically relevant proteins which contain fluorinated amino acids at specific sites by means of a cell-free translation system. Such engineered proteins are expected to contribute to our understanding of discrete fluorines' interaction with canonical amino acids in a native protein environment and to enable the design of fluorinated proteins with arbitrary desired properties.
Ye
Shijie
S
Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Institute of Chemistry and Biochemistry - Organic Chemistry, Takustrasse 3, 14195 Berlin, Germany.
Ann Berger
Allison
A
Petzold
Dominique
D
Reimann
Oliver
O
Matt
Benjamin
B
Koksch
Beate
B