title: [Changes of proteomics in the injured lung of adult rats subjected to acrolein inhalation].
abstract: OBJECTIVE
  To investigate the proteomics change in injured lung tissues of adult rats subjected to acrolein inhalation for 6 weeks.
  
  
  METHODS
  Two-dimensional electrophoresis was used to define the change of proteomic expresses and mass spectrometry was applied to identify the amino acid sequence in differential expressional proteins in lung tissues between acrolein inhalation group and saline control group.
  
  
  RESULTS
  A total of 545 protein spots were found in the lung tissues tested. Of the 545 proteins, 7 differentially expressed proteins were detected in acrolein inhalation group as compared with saline control group. Among them, tyrosine 3/tryptophan 5-monooxygenase activation protein, phosphatidylinositol transfer protein, poly(A)-binding protein, presented a marked upregulation, while 2601 and 2603 vimentin, phosphatidylinositol transfer protein, and chloride intracellular channel 3 showed a marked downregulation.
  
  
  CONCLUSIONS
  This study provides the crucial evidence that differential expressional proteins may underline the machenism of lung injury after acrolein inhalation in adult rats.
authors.affiliation: Department of Respiration, the First People' s Hospital of Yunnan Province, Kunming 65000, China.
authors.forename: Qi-qin
authors.initials: QQ
authors.lastname: Dan
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Shan
authors.initials: S
authors.lastname: Zhao
authors.forename: Sheng-lan
authors.initials: SL
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Yuan
authors.forename: Yun-hui
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D000171:Acrolein / Q000008:administration & dosage / Q000633:toxicity*; D000280:Administration, Inhalation; D000818:Animals; D005260:Female; D008168:Lung / Q000378:metabolism / Q000473:pathology; D055370:Lung Injury / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D049294:Phospholipid Transfer Proteins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D049294:Phospholipid Transfer Proteins; D020543:Proteome; D000171:Acrolein; D014446:Tyrosine 3-Monooxygenase