title: Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system.
abstract: The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis-regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large-scale analysis of enhancer elements, site-specifically integrated into the Drosophila genome. This system, which is scalable for either small projects or high-throughput approaches, makes use of the Gateway cloning technology and the PhiC31 site-specific integration system, which allows the insertion of constructs at predetermined genomic locations. Thus, this system allows not only a fast and easy analysis of reporter gene expression in live animals, but also the simultaneous analysis of different regulatory outputs on a cellular resolution by recombining in the same animal distinct enhancer elements fused to different fluorescent proteins.
authors.affiliation: CellNetworks-Cluster of Excellence and BIOQUANT Center, University of Heidelberg, D-69120 Heidelberg, Germany.
authors.forename: Aurelia L
authors.initials: AL
authors.lastname: Boy
authors.forename: Zongzhao
authors.initials: Z
authors.lastname: Zhai
authors.forename: Anette
authors.initials: A
authors.lastname: Habring-Müller
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Kussler-Schneider
authors.forename: Petra
authors.initials: P
authors.lastname: Kaspar
authors.forename: Ingrid
authors.initials: I
authors.lastname: Lohmann
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001665:Binding Sites; D003001:Cloning, Molecular / Q000379:methods*; D004331:Drosophila melanogaster / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D057166:High-Throughput Screening Assays / Q000379:methods*; D019426:Integrases / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic
chemical_list: D011993:Recombinant Fusion Proteins; D019426:Integrases