title: Comparison of methods of detecting bacterial biofilm.
abstract: Fresh bacterial biofilm produced by Staphylococcus epidermidis was sensitively detected by using a chemiluminescent probe. Its lower detection limit was around 10(-5) - 10(-4) dilution (equivalent to 10(-5) - 10(-4) cm2 biofilm) - 10(2) - 10(3) times as sensitive as ordinary colorimetric methods. The linearity and dynamic range were slightly better than those of fluorometry.
authors.affiliation: Legal Medicine and Molecular Genetics, Postgraduate School of Medicine, Gunma University, Maebashi 371-8511, Japan. y-tajima@koaikai.or.jp
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tajima
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Rie
authors.initials: R
authors.lastname: Takagi
authors.forename: Tamiko
authors.initials: T
authors.lastname: Nakajima
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Kominato
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D003124:Colorimetry; D003470:Culture Media; D005109:Extracellular Matrix / Q000502:physiology; D005453:Fluorescence; D049449:Luminescence; D012680:Sensitivity and Specificity; D013212:Staphylococcus epidermidis / Q000502:physiology*; D014909:Wheat Germ Agglutinins / Q000032:analysis
chemical_list: D003470:Culture Media; D014909:Wheat Germ Agglutinins