Trafficking of UL37 proteins into mitochondrion-associated membranes during permissive human cytomegalovirus infection.
Human cytomegalovirus (HCMV) UL37 proteins traffic sequentially from the endoplasmic reticulum (ER) to the mitochondria. In transiently transfected cells, UL37 proteins traffic into the mitochondrion-associated membranes (MAM), the site of contact between the ER and mitochondria. In HCMV-infected cells, the predominant UL37 exon 1 protein, pUL37x1, trafficked into the ER, the MAM, and the mitochondria. Surprisingly, a component of the MAM calcium signaling junction complex, cytosolic Grp75, was increasingly enriched in heavy MAM from HCMV-infected cells. These studies show the first documented case of a herpesvirus protein, HCMV pUL37x1, trafficking into the MAM during permissive infection and HCMV-induced alteration of the MAM protein composition.
Bozidis
Petros
P
Center for Cancer and Immunology Research, Children's Research Institute, Children's National Medical Center, Washington, DC 200101, USA.
Williamson
Chad D
CD
Wong
Daniel S
DS
Colberg-Poley
Anamaris M
AM
D002478:Cells, Cultured; D003586:Cytomegalovirus Infections / Q000473:pathology* / Q000821:virology*; D004721:Endoplasmic Reticulum / Q000737:chemistry; D005347:Fibroblasts / Q000821:virology; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D017874:Immediate-Early Proteins / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis; D051336:Mitochondrial Membranes / Q000737:chemistry*; D021381:Protein Transport
D018840:HSP70 Heat-Shock Proteins; D017874:Immediate-Early Proteins; D008565:Membrane Proteins; C420323:UL37 protein, Human herpesvirus 5; C018551:glucose-regulated proteins