title: Wingless modulates the ligand independent traffic of Notch through Dishevelled.
abstract: Here we investigate the structural and functional basis of the interactions between Notch and Wingless signalling in Drosophila. Using yeast-two-hybrid and pull-down assays we show that Notch can bind directly a form of Dishevelled that is stabilized upon Wingless signalling. Moreover, we show that the mechanism by which Wingless signalling is able to downregulate Notch is by promoting its ligand-independent traffic to a compartment where it is degraded and that this activity depends on Dishevelled.
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge, UK.
authors.forename: Silvia
authors.initials: S
authors.lastname: Muñoz-Descalzo
authors.forename: Phil G T
authors.initials: PG
authors.lastname: Sanders
authors.forename: Chrystelle
authors.initials: C
authors.lastname: Montagne
authors.forename: Ruth I
authors.initials: RI
authors.lastname: Johnson
authors.forename: Tina
authors.initials: T
authors.lastname: Balayo
authors.forename: Alfonso Martinez
authors.initials: AM
authors.lastname: Arias
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D000818:Animals; D000072261:Dishevelled Proteins; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism* / Q000502:physiology*; D005260:Female; D008024:Ligands; D008297:Male; D010750:Phosphoproteins / Q000502:physiology*; D021381:Protein Transport / Q000502:physiology; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D051155:Wnt1 Protein / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000072261:Dishevelled Proteins; D029721:Drosophila Proteins; D008024:Ligands; C497759:N protein, Drosophila; D010750:Phosphoproteins; D051880:Receptors, Notch; D051155:Wnt1 Protein; C000606588:dsh protein, Drosophila; C074622:wg protein, Drosophila