Expansion of genes encoding a novel type of dynamin in the genome of the pea aphid, Acyrthosiphon pisum.
Screening of the entire genome of the pea aphid, Acyrthosiphon pisum, detected 15 genes for putative dynamin superfamily proteins - self-assembling large GTPases that are involved in the fission and fusion of membranes. In addition to a single gene each for Dyn, Drp1, and Opa1, orthologues that are common in Metazoa, 12 genes encoding a novel type of dynamin were found. Phylogenetic analyses showed that these novel-class genes are monophyletic. Quantitative reverse transcription-PCR demonstrated that expressions of four novel-class dynamin genes are highly up-regulated in the midgut, through which aphids take in phloem-sap diets and plant viruses. As this type of dynamin is absent from all other fully sequenced organisms, they may function in processes unique to aphids.
Nakabachi
A
A
Advanced Science Institute, RIKEN, Wako, Saitama, Japan. bachi@riken.jp <bachi@riken.jp>
Miyagishima
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000201:enzymology / Q000235:genetics* / Q000821:virology; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D034281:Dynamins / Q000235:genetics*; D015870:Gene Expression; D017344:Genes, Insect; D049750:Genome, Insect; D019476:Insect Proteins / Q000235:genetics*; D007303:Insect Vectors / Q000821:virology; D008969:Molecular Sequence Data; D005810:Multigene Family; D018532:Pisum sativum / Q000469:parasitology / Q000821:virology; D010802:Phylogeny; D010942:Plant Viruses; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D019476:Insect Proteins; D034281:Dynamins