title: Gankyrin promotes the proliferation of human pancreatic cancer.
abstract: Previous studies in our laboratory have suggested that gankyrin expression is correlated with a malignant phenotype in colorectal cancer. Here, we investigated the possible role of gankyrin in pancreatic carcinogenesis. Gankyrin expression was significantly increased in pancreatic cancer compared to non-cancerous tissues. This expression significantly enhanced cancer cell proliferation and growth in vitro and in vivo. Suppression of gankyrin downregulated cyclin A, cyclin D1, cyclin E, CDK2, CDK4, PCNA and p-Rb but upregulated p27, Rb and p53. However, gankyrin overexpression led to opposite results. Thus, gankyrin could enhance pancreatic cancer cell proliferation by promoting cell cycle progression and p53 degradation.
authors.affiliation: Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Meng
authors.forename: Lijie
authors.initials: L
authors.lastname: He
authors.forename: Xuegang
authors.initials: X
authors.lastname: Guo
authors.forename: Shanhong
authors.initials: S
authors.lastname: Tang
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Zhao
authors.forename: Rui
authors.initials: R
authors.lastname: Du
authors.forename: Jiang
authors.initials: J
authors.lastname: Jin
authors.forename: Qian
authors.initials: Q
authors.lastname: Bi
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Nie
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
mesh_terms: D000818:Animals; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019925:Cyclin A / Q000378:metabolism; D019938:Cyclin D1 / Q000378:metabolism; D019927:Cyclin E / Q000378:metabolism; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009367:Neoplasm Staging; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D016160:Retinoblastoma Protein / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C530104:CCND1 protein, human; D019925:Cyclin A; D019927:Cyclin E; C403591:PSMD10 protein, human; D018809:Proliferating Cell Nuclear Antigen; D011518:Proto-Oncogene Proteins; D016160:Retinoblastoma Protein; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27; C495894:CDK2 protein, human; C495900:CDK4 protein, human; D051357:Cyclin-Dependent Kinase 2; D051358:Cyclin-Dependent Kinase 4; D046988:Proteasome Endopeptidase Complex