title: Detection of all species of the genus Alphavirus by reverse transcription-PCR with diagnostic sensitivity.
abstract: Clinical arbovirus screening requires exclusion of a broad range of viruses with as few assays as possible. We present a reverse transcription-PCR (RT-PCR) for the detection of all species of the genus Alphavirus qualified for exclusion screening (limit of detection [LOD], 5 to 100 RNA copies per reaction across all Alphavirus species; detection of viremia down to ca. 10,000 copies per ml).
authors.affiliation: Institute of Virology, University of Bonn, Bonn, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Grywna
authors.forename: Bernd
authors.initials: B
authors.lastname: Kupfer
authors.forename: Marcus
authors.initials: M
authors.lastname: Panning
authors.forename: Jan Felix
authors.initials: JF
authors.lastname: Drexler
authors.forename: Petra
authors.initials: P
authors.lastname: Emmerich
authors.forename: Christian
authors.initials: C
authors.lastname: Drosten
authors.forename: Beate M
authors.initials: BM
authors.lastname: Kümmerer
mesh_terms: D000524:Alphavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D018354:Alphavirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*