Autocrine motility factor receptor signaling pathway promotes cell invasion via activation of ROCK-2 in esophageal squamous cell cancer cells.
Aberrant overexpression of autocrine motility factor and its receptor (AMFR) is observed in many cancers but not in esophageal squamous cell cancer (ESCC). In this study, upregulated rho-associated protein kinase 2, p-cofilin and intracellular adhesion molecule-1, and downregulated E-cadherin were found in ESCC cells transfected with the plasmid pcDNA 3.1-AMFR-C, while opposite results were observed in ESCC cells transfected with siRNA against AMFR. Additionally, an elevated invasion of ESCC cells by AMFR was reversed by rho-associated protein kinase 2 inhibitor Y-27632, suggesting that AMFR pathway promotes invasion of ESCC cells and may be a potential target for ESCC therapy.
Wang
Lili
L
Institute of Tumor Molecular Surgery, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
Hou
Guiqin
G
Xue
Lexun
L
Li
Jie
J
Wei
Pan
P
Xu
Peirong
P
D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D004789:Enzyme Activation / Q000502:physiology; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006801:Humans; D009361:Neoplasm Invasiveness / Q000473:pathology; D060285:Receptors, Autocrine Motility Factor; D018121:Receptors, Cytokine / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D054460:rho-Associated Kinases / Q000378:metabolism*
D018121:Receptors, Cytokine; C555000:AMFR protein, human; D060285:Receptors, Autocrine Motility Factor; D044767:Ubiquitin-Protein Ligases; C515852:ROCK2 protein, human; D054460:rho-Associated Kinases