title: SiRNA-loaded multi-shell nanoparticles incorporated into a multilayered film as a reservoir for gene silencing.
abstract: In this study, we presented a new type of coating based on polyelectrolyte multilayers containing sequentially adsorbed active shRNA calcium phosphate nanoparticles for locally defined and temporarily variable gene silencing. Therefore, we investigated multi-shell calcium phosphate-shRNA nanoparticles embedded into a polyelectrolyte multilayer for gene silencing. As model system, we synthesized triple-shell calcium phosphate-shRNA nanoparticles (NP) and prepared polyelectrolyte multilayers films made of nanoparticles and poly-(L-lysine) (PLL). The biological activities of these polyelectrolyte multilayers films were tested by the production of osteopontin and osteocalcin in the human osteoblasts (HOb) which were cultivated on the PEM films. This new strategy can be used to efficiently control the bone formation and could be applicable in tissue engineering.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, INSERM, Unité 977, Faculté de Médecine, Strasbourg, France.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Anna
authors.initials: A
authors.lastname: Kovtun
authors.forename: Carlos
authors.initials: C
authors.lastname: Mendoza-Palomares
authors.forename: Mustapha
authors.initials: M
authors.lastname: Oulad-Abdelghani
authors.forename: Florence
authors.initials: F
authors.lastname: Fioretti
authors.forename: Simon
authors.initials: S
authors.lastname: Rinckenbach
authors.forename: Didier
authors.initials: D
authors.lastname: Mainard
authors.forename: Matthias
authors.initials: M
authors.lastname: Epple
authors.forename: Nadia
authors.initials: N
authors.lastname: Benkirane-Jessel
mesh_terms: D002478:Cells, Cultured; D020868:Gene Silencing; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D010006:Osteoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D053495:Osteopontin / Q000096:biosynthesis; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D034741:RNA, Small Interfering; D015675:Osteocalcin; D053495:Osteopontin