Inhibition of lindane-induced toxicity using alpha-lipoic acid and vitamin E in the brain of Mus musculus.
In the present investigation, we have used adenosine triphosphatase (ATPase) activity as biochemical test of toxic action of lindane that was explained by lipid peroxidation model. Study was also undertaken to ascertain the potential protective role of alpha-lipoic acid (ALA) and vitamin E on the same parameters. Highly acute dose of lindane, i.e., 40 mg/kg bw for 18 h exposure, was used for creating lesions in brain. Lipid peroxidation was measured in terms of glutathione peroxidase and thio barbituric acid-reacting substances (TBARS). Various brain regions under investigation were cerebellum and pons-medulla oblongata. Healthy, male, Swiss mice (7-8 weeks old) were allocated into four groups. First group was control, second group was treated with lindane, third group was treated purely with antioxidants, and fourth group received both antioxidants and lindane treatment. Results revealed the significant difference (at 1% and 5% in all groups) in all studied parameters from control. Increased TBARS level in second group suggests that lindane enhances the production of free radicals in studied brain regions. Antioxidants under test are efficient remedy for neurotoxicity caused by lindane. We conclude that lindane manifests toxic effects on brain ATPase and enhances lipid peroxidation. ALA and vitamin E in combination may provide protection against lindane-induced acute toxicity.
Bist
Renu
R
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Tonk, Rajasthan, India, 304022. renu_bisht22@yahoo.co.in
Misra
Shrilekha
S
Bhatt
Devendra K
DK
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D002531:Cerebellum / Q000187:drug effects / Q000201:enzymology; D005978:Glutathione / Q000378:metabolism; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D008297:Male; D051379:Mice; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D008063:Thioctic Acid / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D001556:Hexachlorocyclohexane; D008063:Thioctic Acid; D000251:Adenosine Triphosphatases; D005978:Glutathione