title: Turnover of inositol phosphates in brain during ischemia-induced breakdown of polyphosphoinositides.
abstract: Using either [(32)]ATP or [(3)H]inositol as precursors which were injected intraventricularly into rat brain, decapitative ischemic treatment resulted in a more rapid loss of labeled phosphatidylinositol 4,5-biphosphates than phosphatidylinositol 4-phosphates in the initial 30 s-1 min. When polyphosphoinositides were labeled with [(3)H]inositol, the breakdown of these compounds was accompanied by a time-dependent appearance of labeled inositol phosphates. Although the level of radioactivity of inositol trisphosphate was low, a peak labeling activity was shown at 30 s. The radioactivity of inositol bisphosphate showed an increase after a delay of 30 s, and reached a peak at 1 min before declining to the baseline level at 5 min. There was also a lag period of 30 s for the appearance of labeled inositol monophosphate, after which the radioactivity continued to increase in a biphasic manner for the entire 5 min period. Results indicate that decapitative ischemic treatment to rats can serve as an experimental model for assessing in vivo stimulation of the receptor-mediated signal transduction mechanism related to polyphosphoinositide breakdown and subsequent turnover of inositol phosphates in brain.
authors.affiliation: Department of Biochemistry and Sinclair Comparative Medicine Research Farm, University of Missouri, Columbia, MO 65203, U.S.A.
authors.forename: G Y
authors.initials: GY
authors.lastname: Sun
authors.forename: H M
authors.initials: HM
authors.lastname: Huang
authors.forename: R
authors.initials: R
authors.lastname: Chandrasekhar