title: Measurement of sVEGF R1 and PlGF in serum: comparing prototype assays from Beckman Coulter, Inc. to R&D Systems microplate assays.
abstract: OBJECTIVE
  To compare the performance of prototype Access® sVEGF R1 and PlGF automated immunoassays from Beckman Coulter to the Quantikine® microplate ELISA assays by R&D Systems.
  
  
  METHODS
  Samples obtained from pregnant women, non-pregnant women and men were assayed according to manufacturers' instructions.
  
  
  RESULTS
  Compared to the Quantikine assays, the Access assays demonstrated improved precision, increased sensitivity, broader dynamic ranges, and reduced analysis time. The Access assays were found to be specific for free sVEGF R1 and free PlGF.
  
  
  CONCLUSIONS
  There was good correlation between the Access and Quantikine assays. Superior performance by Access assays may have important prenatal diagnostic implications.
authors.affiliation: Abbott Northwestern Hospital, Minneapolis, MN, USA.
authors.forename: Donald
authors.initials: D
authors.lastname: Wothe
authors.forename: Emanuel
authors.initials: E
authors.lastname: Gaziano
authors.forename: Shiraz
authors.initials: S
authors.lastname: Sunderji
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Kusanovic
authors.forename: Linda
authors.initials: L
authors.lastname: Rogers
authors.forename: Cheryl
authors.initials: C
authors.lastname: Hodges-Savola
authors.forename: Sean
authors.initials: S
authors.lastname: Roberts
authors.forename: James
authors.initials: J
authors.lastname: Wassenberg
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D000072483:Placenta Growth Factor; D011225:Pre-Eclampsia / Q000097:blood / Q000175:diagnosis; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood*; D012680:Sensitivity and Specificity; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood*
chemical_list: C000608036:PGF protein, human; D011257:Pregnancy Proteins; D000072483:Placenta Growth Factor; D040281:Vascular Endothelial Growth Factor Receptor-1