Solid free-form fabrication-based PCL/HA scaffolds fabricated with a multi-head deposition system for bone tissue engineering.
In this study, we fabricated polycaprolactone/hydroxyapatite (PCL/HA) scaffolds with a multi-head deposition system, a solid free-form fabrication technology that was developed in our previous study. The bone regeneration potential of the scaffolds was compared with that of PCL scaffolds fabricated with the same system. The fabricated scaffolds had a pore size of 400 mum and a porosity of 66.7%. The PCL/HA scaffolds had higher mechanical strength and modulus than the PCL scaffolds. To compare the osteogenic potential, the two types of scaffolds were seeded with rat osteoblasts and cultured in vitro or implanted subcutaneously into athymic mice. The cells cultured on PCL/HA scaffolds expressed higher levels of osteopontin and osteonectin, both of which are osteogenic proteins. The PCL/HA scaffolds resulted in larger bone area and calcium deposition in the implants compared to the PCL scaffolds.
Kim
Jong Young
JY
Department of Mechanical Engineering, Andong National University, Andong, Gyunbuk 760-749, South Korea.
Lee
Tae-Jin
TJ
Cho
Dong-Woo
DW
Kim
Byung-Soo
BS
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry*; D005260:Female; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite