Convergence in probiotic Lactobacillus gut-adaptive responses in humans and mice.
Probiotic bacteria provide unique opportunities to study the global responses and molecular mechanisms underlying the effects of gut-associated microorganisms in the human digestive tract. In this study, we show by comparative transcriptome analysis using DNA microarrays that the established probiotic Lactobacillus plantarum 299v specifically adapts its metabolic capacity in the human intestine for carbohydrate acquisition and expression of exopolysaccharide and proteinaceous cell surface compounds. This report constitutes the first application of global gene expression profiling of a commensal microorganism in the human gut. A core L. plantarum transcriptome expressed in the mammalian intestine was also determined through comparisons of L. plantarum 299v activities in humans to those found for L. plantarum WCFS1 in germ-free mice. These results identify the niche-specific adaptations of a dietary microorganism to the intestinal ecosystem and provide novel targets for molecular analysis of microbial-host interactions which affect human health.
Marco
Maria L
ML
TI Food and Nutrition, Wageningen, The Netherlands.
de Vries
Maaike C
MC
Wels
Michiel
M
Molenaar
Douwe
D
Mangell
Peter
P
Ahrne
Siv
S
de Vos
Willem M
WM
Vaughan
Elaine E
EE
Kleerebezem
Michiel
M
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis; D050260:Carbohydrate Metabolism; D041981:Gastrointestinal Tract / Q000382:microbiology*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D019936:Probiotics / Q000008:administration & dosage*; D013312:Stress, Physiological
D001426:Bacterial Proteins; D008565:Membrane Proteins; D011135:Polysaccharides, Bacterial