Characterisation of microbial communities in relation to physical-chemical parameters during in situ aeration of waste material.
This study investigates changes in waste microbial community composition and biomass during in situ aeration in laboratory-scale columns over 32 weeks. Microbial profiles were assessed in solid and leachate samples in relation to physical-chemical parameters using phospholipid ester linked fatty acid (PLFA) and phospholipid ether lipid (PLEL) analysis and parameters such as pH, EC, TC, TOC, TN, NO(3)(-), NH(4)(+), COD and the biochemical parameter BOD(5). Principal component analysis (PCA) of the individual PLFAs and PLELs indicated a change in community composition and biomass over the operation period, which could be differentiated in the three phases (i) anaerobic, (ii) aeration start and (iii) extended aeration. PCA revealed that aeration and pH values were the most influential parameters on microbial dynamics. There was a marked decrease of ubiquitous microorganisms, some Gram negative bacterial groups and methanogenic archaea, but a consecutive increase of Gram positive microbial groups along with a rapid reduction of organics after aeration start. Those in situ aeration effects on microbial community composition and C conversion were stable throughout the laboratory set-up of 32 weeks.
Mellendorf
Maren
M
Institute of Soil Research, Department of Forest and Soil Sciences, University of Natural Resources and Applied Life Sciences, Peter-Jordan Strasse 82, 1190 Vienna, Austria. maren.mellendorf@boku.ac.at
Huber-Humer
Marion
M
Gamperling
Oliver
O
Huber
Pia
P
Gerzabek
Martin H
MH
Watzinger
Andrea
A
D000388:Air; D000393:Air Pollutants / Q000032:analysis; D001105:Archaea; D018533:Biomass; D002627:Chemistry, Physical / Q000379:methods; D004784:Environmental Monitoring; D004987:Ethers / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D012037:Refuse Disposal / Q000379:methods*; D012988:Soil Microbiology
D000393:Air Pollutants; D004987:Ethers; D008055:Lipids; D010743:Phospholipids