The lipid requirements of mediatophore for acetylcholine release activity. Large-scale purification of this protein in a reactive form.
The mediatophore, a protein which translocates acetylcholine, has been recently purified from the plasma membrane of the Torpedo electric organ nerve terminals. The functional integrity of the mediatophore requires the presence of lipids. Removal of associated lipids led to an irreversible loss of activity when obtained by ether precipitation of the protein. A two-phase extraction procedure was developed to gently remove the lipids. Conditions were found to reactivate the protein by emulsifying it with electric organ lipids. A large-scale preparation procedure of the delipidified mediatophore in a reactive form is described.
Israël
M
M
Département de Neurochimie, Laboratoire de Neurobiologie Cellulaire et Moléculaire, C.N.R.S. 91198 Gif sur Yvette, Cedex, France.
Lesbats
B
B
Morel
N
N
Manaranche
R
R
Birman
S
S