title: A role of piperine on monosodium urate crystal-induced inflammation--an experimental model of gouty arthritis.
abstract: In the present study, the anti-inflammatory effect of piperine was investigated on monosodium urate crystal-induced inflammation in mice, an experimental model for gouty arthritis, and compared it with that of the nonsteroidal anti-inflammatory drug, indomethacin. The levels of lysosomal enzymes, lipid peroxidation, tumor necrosis factor-α, and paw volume were increased significantly, and the activities of antioxidant status were in turn decreased in monosodium urate crystal-induced mice, whereas these changes were reverted to near normal levels upon piperine (30 mg/kg b.wt, i.p.) treatment. In vitro, piperine (50/100 ug/ml) suppressed the level of β-glucuronidase and lactate dehydrogenase in monosodium urate crystal-incubated polymorphonuclear leucocytes in concentration-dependent manner when compared to control cells. Thus, the present study clearly indicated that piperine inhibit the monosodium urate crystal-induced inflammation and can be regarded as therapeutic drug for the treatment of acute gouty arthritis.
authors.affiliation: School of Bio Sciences and Technology, VIT University, Vellore, 632 014, Tamil Nadu, India.
authors.forename: Evan Prince
authors.initials: EP
authors.lastname: Sabina
authors.forename: Shruthi
authors.initials: S
authors.lastname: Nagar
authors.forename: Mahaboobkhan
authors.initials: M
authors.lastname: Rasool
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D015210:Arthritis, Gouty / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D052117:Benzodioxoles / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology; D010880:Piperidines / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014527:Uric Acid / Q000633:toxicity
chemical_list: D000470:Alkaloids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D052117:Benzodioxoles; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D014409:Tumor Necrosis Factor-alpha; D014527:Uric Acid; C008922:piperine; D007213:Indomethacin