title: Optically induced linking of protein and nanoparticles to gold surfaces.
abstract: Attachment of molecules and proteins to surfaces is of great interest for the development of a large variety of applications. We present herein a novel approach to efficiently couple a molecule of choice to biological building blocks. We synthesized and employed a new derivative of 5-bromo-7-nitroindoline to attach nucleophilic molecules and proteins to gold surfaces by photochemical activation. The reaction can be seen as a photoactivated alternative to the activated ester type chemistries that are commonly used to attach proteins or molecules to surfaces. We characterize the reaction by UV-vis and NMR spectroscopy, and as test of principle experiment, we show that we can attach proteins to surfaces and demonstrate that we can functionalize gold nanoparticles by this optically induced cross-linking reaction.
authors.affiliation: Nano-Science Center, Department of Chemistry, University of Copenhagen, Denmark.
authors.forename: Kasper
authors.initials: K
authors.lastname: Moth-Poulsen
authors.forename: Vesna
authors.initials: V
authors.lastname: Kofod-Hansen
authors.forename: Fadhil S
authors.initials: FS
authors.lastname: Kamounah
authors.forename: Nikos S
authors.initials: NS
authors.lastname: Hatzakis
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Stamou
authors.forename: Kjeld
authors.initials: K
authors.lastname: Schaumburg
authors.forename: Jørn B
authors.initials: JB
authors.lastname: Christensen
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry*; D004952:Esters / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006842:Hydrocarbons, Brominated / Q000737:chemistry; D007211:Indoles / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D053768:Metal Nanoparticles / Q000737:chemistry*; D009574:Nitro Compounds / Q000737:chemistry; D010316:Particle Size; D010777:Photochemistry; D011506:Proteins / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties
chemical_list: D003432:Cross-Linking Reagents; D004952:Esters; D006842:Hydrocarbons, Brominated; D007211:Indoles; D009574:Nitro Compounds; D011506:Proteins; C057812:indoline; D006046:Gold