title: [MicroRNA profiling in patients with hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate the differential expression of microRNAs between human hepatocellular carcinoma (HCC) and liver cirrhosis (LC).
  
  
  METHODS
  The total RNA was extracted from 25 pairs of HCC and LC tissues. microRNA microarray was used to analyze the microRNA expression profiles, and validation of the microarray results was carried out by real-time quantitative RT-PCR (qRT-PCR).
  
  
  RESULTS
  Three microRNAs exhibited higher expression in the HCC samples than in the LC samples. In comparison with the LC samples, the HCC samples showed down-regulated expressions of 9 microRNAs. qRT-PCR verified that has-miR-122a, has-miR-199a, and has-miR-199b were downregulated in HCC, which was in agreement with the microarray results.
  
  
  CONCLUSIONS
  HCC and LC samples have significantly different microRNA expression profiles. These differentially expressed microRNAs may be involved in the pathogensis of HCC.
authors.affiliation: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. chuanxiwang@126.com
authors.forename: Chuan-xi
authors.initials: CX
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Zeng-jun
authors.initials: ZJ
authors.lastname: Li
authors.forename: Bao
authors.initials: B
authors.lastname: Song
authors.forename: De-hua
authors.initials: DH
authors.lastname: Wu
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Sun
authors.forename: Long-hua
authors.initials: LH
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008103:Liver Cirrhosis / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged
chemical_list: D035683:MicroRNAs