[The expression of TWEAKR/Fn14 in Rheumatoid arthritis fibroblast-like synoviocytes].
OBJECTIVE
To investigate the mechanism of TWEAK on Rheumatoid arthritis fibroblast-like synoviocytes (RA FLS) activation and articular destruction by analyzing expression of TWEAKR/Fn14 on RA FLS.


METHODS
FLS was co-cultured with rhTWEAK, with TNF-alpha or IL-1beta as a synergistic stimulator. Expression of TWEAKR/Fn14 protein on RA FLS was detected by Immunocytochemistry and Western blotting. The fn14 mRNA was measured by RT-PCR.


RESULTS
TWEAKR/Fn14 was expressed on the cell membrane and in the cytoplasm of RA FLS. TWEAKR/Fn14 mRNA and protein induced by 100 microg/L TWEAK were higher than the control group. TNF-alpha and IL-1beta had synergistic effect on expression of TWEAKR/Fn14 in RA FLS.


CONCLUSIONS
TWEAKR/Fn14 was expressed on the cell membrane and in the cytoplasm of RA FLS, and recombinant TWEAK further enhanced the TWEAKR/Fn14 expression. TNF-alpha and IL-1beta had synergistic effect on the biological activity of TWEAK by increasing the expression of TWEAKR/Fn14 on RA FLS.
Xia
Li-Ping
LP
Department of Rheumatic Immunology, First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Fang
Fang
F
Lu
Jing
J
Xiao
Wei-Guo
WG
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000494:pharmacology; D051379:Mice; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism*; D000074050:TWEAK Receptor; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D053583:Interleukin-1beta; D018124:Receptors, Tumor Necrosis Factor; C000613297:TNFRSF12A protein, human; D000074050:TWEAK Receptor; C000613299:Tnfrsf12a protein, mouse; D014409:Tumor Necrosis Factor-alpha