[Lipoprotein lipase expression in the hippocampus and its effects on vitamin E levels in rats with epilepsy].
OBJECTIVE
To investigate the dynamic changes of lipoprotein lipase (LPL) expression in the hippocampus of epileptic rats and to study its effect on vitamin E levels in rats following status epilepticus (SE).


METHODS
Rat model of SE was induced by intraperitoneal injection of pilocarpine. The rats receiving an injection of normal saline were used as a control group. The expression of LPL in the hippocampal tissue was determined using immunofluorescent methods and the level of vitamin E was examined by the colormeric method 12 hrs, 24 hrs, 3 days, 7 days and 14 days after SE.


RESULTS
LPL was expressed in the control and SE groups. In the SE group, the LPL expression began to increase 24 hr after SE (P<0.05), reached a peak 3 days after SE (P<0.01), and kept at a high level 7 days after SE (P<0.01). By 14 days, the LPL expression was reduced to the level similar to the control group. The level of vitamin E began to decline 12 hrs after SE (P<0.01), and decreased to a nadir 24 hrs after SE (P<0.01). At 3 and 7 days after SE, the levels of vitamin E were still significantly lower than the controls (P<0.05). By 14 days, the vitamin E level increased to the level similar to the control group.


CONCLUSIONS
The over-expression of LPL in the hippocampus may play an important role in the oxidative stress mechanisms following SE by regulating the uptake of vitamin E.
Shi
Xuan
X
Department of Pediatrics, People's Hospital of Wuhan University, Wuhan, China.
Yao
Bao-Zhen
BZ
Liu
Dan
D
D000818:Animals; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000378:metabolism*; D008071:Lipoprotein Lipase / Q000032:analysis / Q000502:physiology*; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D013226:Status Epilepticus / Q000378:metabolism*; D014810:Vitamin E / Q000032:analysis / Q000378:metabolism*
D014810:Vitamin E; D008071:Lipoprotein Lipase