Identification of X chromosome copies by quantitative real-time polymerase chain reaction for population screening tests.
We adapted a method that allows the identification of X chromosome copy number by quantitative real-time polymerase chain reaction (PCR) in a short amount of time. It may be used for sex and aneuploidy involving X chromosome large population screening tests.
Ramos
Ester S
ES
Department of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. esramos@rge.fmrp.usp.br
Serafim
Juliana V
JV
Takeuchi
Paula L
PL
Marcondes
Cintia R
CR
Araújo
Adriane
A
D000782:Aneuploidy; D016022:Case-Control Studies; D058674:Chromosome Duplication; D041321:Chromosomes, Human, X / Q000235:genetics; D005260:Female; D018628:Gene Dosage; D005820:Genetic Testing / Q000379:methods*; D005828:Genetics, Population / Q000379:methods; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012729:Sex Chromosome Aberrations; D025064:Sex Chromosome Disorders / Q000175:diagnosis / Q000235:genetics