title: [Determination of serum copper and zinc in different chemical forms by graphite furnace atomic absorption spectrometry with ethanol-EDTA precipitation method].
abstract: A simple and reliable method was developed for the determination of serum copper and zinc in different chemical forms by graphite furnace atomic absorption spectrometry (GFAAS) with ethanol-EDTA precipitation. The serum and ethanol were mixed with volume ratio 1 : 2. The mixture was incubated at 70 degrees C and ultra-centrifuged to precipitate proteins. Zinc and copper can be released from albumin after EDTA treating. Thus, macroglobulin-zinc, ceruloplasmin-copper, and albumin-bound zinc or copper could be determined by two proposed precipitation steps. The determination limit of copper (3sigma) was 1.2 microg x L(-1) and the recovery was 92.3%-104%, while the determination limit of zinc (3sigma) was 0.098 microg x L(-1), and the recovery was 90%-107%. This two-step precipitation method can be used to determine serum zinc and copper in various chemical forms in tumor, Wilson patients and heathy human.
authors.affiliation: Central Laboratories, Medical College of Shantou University, Shantou 515041, China. yzhang@stu.edu.cn
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhe-Xuan
authors.initials: ZX
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Peng
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Luo
authors.forename: Wei-Qiu
authors.initials: WQ
authors.lastname: Li
authors.forename: Wen-Hong
authors.initials: WH
authors.lastname: Luo
mesh_terms: D003300:Copper / Q000097:blood*; D004492:Edetic Acid; D000431:Ethanol; D006108:Graphite; D006801:Humans; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000097:blood*
chemical_list: D000431:Ethanol; D006108:Graphite; D003300:Copper; D004492:Edetic Acid; D015032:Zinc