[NMR-spectroscopy-based metabonomic approach to analysis of Siwutang, a novel prescription, treated blood deficiency in mice].
OBJECTIVE
To discover the characteristic changes of metabolic profiles in C57 mice with cyclophosphamide induced blood deficiency and the effect of Siwutang.


METHODS
An integrated metabonomic study using high-resolution 1H nuclear magnetic resonance (NMR) spectroscopy has been applied to investigate the metabolic profiles of serum, aqueous and lipidic extracts of thoracic gland, spleen, bone marrow obtained from control, model group (intraperitoneal injection of cyclophosphamide at a dose of 250 mg x kg(-1)) and Siwutang treated model group. The NMR spectra were integrated in segments of 0.04 ppm and then analyzed by principal component analysis (PCA) using SIMCA-P software to visualize the similarities and differences in metabolic profiles between these groups.


RESULTS
PCA result showed conspicuous difference in the metabolic profiles between groups. Compared with the control group, the model group contained lower concentration of lactate, 3-hydroxybutyrate, choline, glucose, and higher concentration of VLDL/LDL, leucine/isoleucine in serum. Lower concentration of taurine choline, Fbeta:RCH2CH2CO, Epi-coprostanol and lactate were found in both in thoracic gland extracts and spleen extracts. And in spleen extracts, we also found the lower concentration of 3-HB. In the extracts of bone marrow, the lower concentration of lactate, choline, glucosee were observed. When they were dosed with Siwutang 10 g x kg(-1) x d(-1) for 7 days, the effects above-mentioned were reversed.


CONCLUSIONS
The injury established by injecting CTX is a kind of proper model to develop further metabonomics researches. The damage of mitochondria, disorder of energy metabolism and osmoregulation are observed in cyclophosphamide caused blood deficiency model by NMR-based-metabonomics method, and the Siwutang can improve these effects.
Wang
Mu
M
Beijing Institute of Radiation Medicine, Beijing 100850, China. wangmu2009@163.com
Rang
Weiqing
W
Zhang
Qi
Q
Huo
Chao
C
Ma
Zengchun
Z
Wang
Yuguang
Y
Yan
Xianzhong
X
Gao
Yue
Y
D000740:Anemia / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D003520:Cyclophosphamide / Q000633:toxicity; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008516:Medicine, Chinese Traditional; D055432:Metabolomics; D051379:Mice; D008810:Mice, Inbred C57BL; D029596:Paeonia; D025341:Principal Component Analysis
D004365:Drugs, Chinese Herbal; D003520:Cyclophosphamide