title: Selective microbial aerosolization in biogas demonstrated by quantitative PCR.
abstract: Aerosolization of Bacteria, Archaea, Synergistes, Staphylococcus spp. and Propionibacterium acnes was investigated in situ with quantitative real-time PCR of DNA isolated from sludge and biogases of anaerobic digesters. The data revealed that in biogas, Staphylococcus spp. and P. acnes were, respectively, aerosolized 30 and 220 times more and Archaea and Synergistes, respectively, 8 and 20 times less aerosolized than Bacteria. This is the first demonstration of selective microbial aerosolization for anaerobic digestors microorganisms. This study illustrates the fact that some microbial groups, such as opportunistic pathogens, are more susceptible to be aerosolized, since they use air as a dissemination vector, and that this has to be taken in account when up-grading biogas into natural gas networks.
authors.affiliation: INRA, UR50, Laboratoire de Biotechnologie de l'Environnement, Narbonne, France. marina.moletta@cstb.fr
authors.forename: Marina
authors.initials: M
authors.lastname: Moletta-Denat
authors.forename: Valérie
authors.initials: V
authors.lastname: Bru-Adan
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Delgenes
authors.forename: Jérôme
authors.initials: J
authors.lastname: Hamelin
authors.forename: Nathalie
authors.initials: N
authors.lastname: Wéry
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Godon
mesh_terms: D000336:Aerosols / Q000032:analysis*; D000693:Anaerobiosis / Q000235:genetics; D001105:Archaea / Q000235:genetics* / Q000302:isolation & purification; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification; D056804:Biofuels / Q000382:microbiology*; D015169:Colony Count, Microbial; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction / Q000379:methods*; D012722:Sewage / Q000382:microbiology
chemical_list: D000336:Aerosols; D056804:Biofuels; D012722:Sewage