title: [Expression of IL-1 mRNA in the dentate gyrus of adult rats following lithium-pilocarione-induced seizures].
abstract: OBJECTIVE
  To examine the expression of IL-1beta, IL-1ra and IL-1R mRNA in the dentate gyrus of adult rats after lithium-pilocarpine (Li-PILO)-induced seizures.
  
  
  METHODS
  Seizure epilepticus (SE) was induced using Li-PILO intraperitoneally (i.p.) injection. Lithium chloride was injected 18-20 h prior to PILO. The rats in the control group were given physiological saline instead of pilocarpine. We observed the behavior of rats and examined the expression of cytokines mRNA in dentate gyrus by RT-PCR.
  
  
  RESULTS
  Rats had seizure epilepticus in 30 min after administrated of pilocarpine. Cytokines (IL-1beta, IL-1ra and IL-1R) began to increase significantly (P<0.05) at 1 h after SE, and the peak was at 6-12 h. Expression of all cytokines declined on 48 h after SE.
  
  
  CONCLUSIONS
  Our findings suggested that SE lead to increased expression of IL-1, IL-1ra and IL-1R1 after lithium-pilocarpine-induced seizures, indicating that cytokines involved in SE and brain impairment after seizure.
authors.affiliation: Department of Neurology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China. zhangshijun2007@sina.com
authors.forename: Shi-jun
authors.initials: SJ
authors.lastname: Zhang
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Dong
authors.initials: D
authors.lastname: Wei
authors.forename: Wen
authors.initials: W
authors.lastname: Jiang
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000187:drug effects / Q000378:metabolism*; D004195:Disease Models, Animal; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics* / Q000378:metabolism; D008094:Lithium / Q000009:adverse effects*; D008297:Male; D010862:Pilocarpine / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000378:metabolism; D012640:Seizures / Q000235:genetics* / Q000378:metabolism
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D017472:Receptors, Interleukin-1; D010862:Pilocarpine; D008094:Lithium