Sugar transporters of the major facilitator superfamily in aphids; from gene prediction to functional characterization.
Analysis of the pea aphid (Acyrthosiphon pisum) genome using signatures specific to the Major Facilitator Superfamily (Pfam Clan CL0015) and the Sugar_tr family (Pfam Family PF00083) has identified 54 genes encoding potential sugar transporters, of which 38 have corresponding ESTs. Twenty-nine genes contain the InterPro IPR003663 hexose transporter signature. The protein encoded by Ap_ST3, the most abundantly expressed sugar transporter gene, was functionally characterized by expression as a recombinant protein. Ap_ST3 acts as a low-affinity uniporter for fructose and glucose that does not depend on Na(+) or H(+) for activity. Ap_ST3 was expressed at elevated levels in distal gut tissue, consistent with a role in gut sugar transport. The A. pisum genome shows evidence of duplications of sugar transporter genes.
Price
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Durham University, UK.
Tibbles
K
K
Shigenobu
S
S
Smertenko
A
A
Russell
C W
CW
Douglas
A E
AE
Fitches
E
E
Gatehouse
A M R
AM
Gatehouse
J A
JA
D000818:Animals; D001042:Aphids / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D005632:Fructose / Q000378:metabolism; D020440:Gene Duplication; D017344:Genes, Insect; D049750:Genome, Insect; D005947:Glucose / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D005810:Multigene Family; D018532:Pisum sativum / Q000469:parasitology; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
D017931:DNA Primers; D019476:Insect Proteins; D009004:Monosaccharide Transport Proteins; D011994:Recombinant Proteins; D005632:Fructose; D005947:Glucose