Characterization of the role of the tumor marker Nup88 in mitosis.
Nuclear pore complexes are massive multiprotein channels responsible for traffic between the nucleus and cytoplasm, and are composed of approximately 30 proteins, termed nucleoporins (Nup). Our recent studies indicated that the nucleoporins Rae1 and Tpr play critical roles in maintaining the spindle bipolarity during cell division. In the present study, we found that another nucleoporin, Nup88, was localized on the spindles together with Nup214 during mitosis. Nup88 expression is linked to the progression of carcinogenesis, Nup88 has been proposed as a tumor marker. Overexpression of Nup88 enhanced multinucleated cell formation. RNAi-mediated knockdown of Nup88 disrupted Nup214 expression and localization and caused multipolar spindle phenotypes. Our data indicate that proper expression of Nup88 is critical for preventing aneuploidy formation and tumorigenesis.
Hashizume
Chieko
C
Frontier Science Organization and Cancer Research Institute, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920-1192 Japan.
Nakano
Hiroshi
H
Yoshida
Kimihisa
K
Wong
Richard W
RW
D000782:Aneuploidy; D014408:Biomarkers, Tumor / Q000032:analysis*; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D042822:Genomic Instability; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D008938:Mitosis / Q000502:physiology*; D009369:Neoplasms / Q000235:genetics*; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D014408:Biomarkers, Tumor; C085914:NUP214 protein, human; C104835:NUP88 protein, human; D028861:Nuclear Pore Complex Proteins; D034741:RNA, Small Interfering