[Hath1 gene transfer inhibits the proliferation of colonic cancer cells in vitro].
OBJECTIVE
To study the effect of Hath1 gene transfer on the proliferation of colonic cancer cells in vitro.


METHODS
The recombinant plasmid pcDNA3.1(+)-Hath1 was transfected into HT29 colonic cancer cells, and 3 positive cell clones were randomly selected to test the levels of Hath1 mRNA, Muc2 mRNA, Hath1, Muc2, cyclin D1 and p27 by quantitative real-time RT-PCR and Western blotting. The proliferation of the transfected HT29 cells was observed by means of colony formation assay and xenograft growth in nude mice.


RESULTS
Hath1 significantly down-regulated of cyclin D1 and up-regulate of p27 expressions and inhibited the proliferation of HT29 cells.


CONCLUSIONS
Hath1 gene may be an anti-oncogene in colon carcinogenesis.
Zhu
Dai-hua
DH
Department of General Surgery, Second Hospital of Chongqing University of Medical Sciences, Chongqing 400010, China. geoffroy324@yahoo.com.cn
Gong
Jian-ping
JP
Ren
Ke
K
Sun
Jian-ming
JM
Wei
Si-dong
SD
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000473:pathology*; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D018014:Gene Transfer Techniques; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D055262:Mucin-2 / Q000235:genetics / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
D051792:Basic Helix-Loop-Helix Proteins; D019938:Cyclin D1; D055262:Mucin-2; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; C500942:ATOH1 protein, human; C530104:CCND1 protein, human; C524625:MUC2 protein, human; C413374:p27 antigen