title: Fluorescence and single molecule analysis in cell biology.
abstract: An overview is presented which describes the development of fluorescence spectroscopy at the cellular level from its beginning as a quantitative tool to determine the content of cellular components to its present use. Analysis of individual biomolecules, their transport and kinetics within a single cell is now possible.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden. rudolf.rigler@ki.se
authors.forename: Rudolf
authors.initials: R
authors.lastname: Rigler
mesh_terms: D000165:Acridine Orange / Q000737:chemistry; D002477:Cells / Q000737:chemistry* / Q000201:enzymology; D004247:DNA / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D012313:RNA; D004247:DNA; D000165:Acridine Orange