[Immunoglobulin quantitation by latex agglutination immunoassay--new sample treatment to reduce differences in measured values by turbidimetric immunoassay].
Serum IgG, IgA, and IgM are routinely measured by turbidimetric immunoassay (TIA), nephelometric immunoassay (NIA), and latex agglutination turbidimetric immunoassay (LATIA) while IgD and IgE, which are present in low concentrations, are usually measured by LATIA and other sensitive assays. There were some differences between LATIA and TIA in the values obtained from lyophilized serum, and sera with monoclonal proteins. We successfully solved the problem by dissociated and/or aggregated immunoglobulins treated with a newly prepared reagent. The difference caused a remarkable improvement thus realizing a coincidence in the measured values on LATIA and TIA.
Kubota
Nobuyuki
N
Compliance and Assurance Division, Eiken Chemical Co., Ltd. Shimotsuga-gun, Tochigi-pref. 329-0114, Japan. nobuyuki_kubota@eiken.co.jp
D015415:Biomarkers / Q000097:blood; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007136:Immunoglobulins / Q000097:blood*; D007202:Indicators and Reagents; D007841:Latex Fixation Tests / Q000379:methods*; D009194:Myeloma Proteins; D009391:Nephelometry and Turbidimetry / Q000379:methods*
D015415:Biomarkers; D007136:Immunoglobulins; D007202:Indicators and Reagents; D009194:Myeloma Proteins