[Expression of IL-1 mRNA in the dentate gyrus of adult rats following lithium-pilocarione-induced seizures].
OBJECTIVE
To examine the expression of IL-1beta, IL-1ra and IL-1R mRNA in the dentate gyrus of adult rats after lithium-pilocarpine (Li-PILO)-induced seizures.


METHODS
Seizure epilepticus (SE) was induced using Li-PILO intraperitoneally (i.p.) injection. Lithium chloride was injected 18-20 h prior to PILO. The rats in the control group were given physiological saline instead of pilocarpine. We observed the behavior of rats and examined the expression of cytokines mRNA in dentate gyrus by RT-PCR.


RESULTS
Rats had seizure epilepticus in 30 min after administrated of pilocarpine. Cytokines (IL-1beta, IL-1ra and IL-1R) began to increase significantly (P<0.05) at 1 h after SE, and the peak was at 6-12 h. Expression of all cytokines declined on 48 h after SE.


CONCLUSIONS
Our findings suggested that SE lead to increased expression of IL-1, IL-1ra and IL-1R1 after lithium-pilocarpine-induced seizures, indicating that cytokines involved in SE and brain impairment after seizure.
Zhang
Shi-jun
SJ
Department of Neurology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China. zhangshijun2007@sina.com
Li
Xiao-wei
XW
Wang
Ying
Y
Wei
Dong
D
Jiang
Wen
W
D000818:Animals; D018891:Dentate Gyrus / Q000187:drug effects / Q000378:metabolism*; D004195:Disease Models, Animal; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics* / Q000378:metabolism; D008094:Lithium / Q000009:adverse effects*; D008297:Male; D010862:Pilocarpine / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000378:metabolism; D012640:Seizures / Q000235:genetics* / Q000378:metabolism
D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D017472:Receptors, Interleukin-1; D010862:Pilocarpine; D008094:Lithium