title: Application of Dolichos biflorus in immunoassay detection of kidney collecting duct biomarkers.
abstract: Currently there are no biomarkers for detecting collecting duct damage in man. Antibodies to several collecting duct-specific antigens exist but sandwich assays have been difficult to establish due to the need for two different antibodies to the same protein. We hypothesized that a collecting duct-specific lectin could be used in combination with a collecting duct-specific antibody to negate the need for two different antibodies. The collecting duct specificity of selected antibodies (NiCa II 13C2, Pap XI 3C7, HuPaP VII 2B11 and aquaporin 2), was verified by immunohistochemistry. Aquaporin 2 and Pap XI 3C7 were used successfully in setting up assays with the lectin Dolichos biflorus, using the Meso Scale Discovery (MSD) platform. Antigen expression was highest in the papillae of rat and human kidney (corresponding to the greatest density of collecting ducts) and was also present in normal urine. We propose that further qualification and validation would lead to an assay for detecting collecting duct damage in man.
authors.affiliation: Safety Assessment, AstraZeneca Pharmaceuticals, Macclesfield, Cheshire, UK.
authors.forename: Samer
authors.initials: S
authors.lastname: Sourial
authors.forename: Lydia
authors.initials: L
authors.lastname: Searchfield
authors.forename: Ina
authors.initials: I
authors.lastname: Schuppe-Koistinen
authors.forename: Graham R
authors.initials: GR
authors.lastname: Betton
authors.forename: Daniela
authors.initials: D
authors.lastname: Riccardi
authors.forename: Sally A
authors.initials: SA
authors.lastname: Price
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000941:Antigens / Q000652:urine; D051399:Aquaporin 2 / Q000276:immunology; D015415:Biomarkers / Q000032:analysis*; D005021:Ethylamines; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007150:Immunohistochemistry; D007668:Kidney / Q000276:immunology / Q000378:metabolism; D007681:Kidney Papillary Necrosis / Q000139:chemically induced / Q000276:immunology / Q000652:urine; D007685:Kidney Tubules, Collecting / Q000276:immunology*; D008297:Male; D037121:Plant Lectins / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000906:Antibodies; D000941:Antigens; D051399:Aquaporin 2; D015415:Biomarkers; D005021:Ethylamines; D037121:Plant Lectins; C036554:dolichos biflorus agglutinin; C004504:2-bromoethylamine