title: Standardized Seidel test to evaluate different sutureless cataract incision configurations.
abstract: OBJECTIVE
  To compare aqueous outflow and India ink inflow to evaluate clear corneal incisions (CCIs).
  
  
  METHODS
  Wilmer Eye Institute, Baltimore, Maryland, USA.
  
  
  METHODS
  Two 25-gauge needles connected to a salt solution bag and to a digital manometer were inserted through the limbus of human donor corneas 120 degrees apart from each other. Three 2.75 mm wide incisions were created in a different third of each cornea as follows: single-plane 1.50 mm tunnel length, single-plane 3.00 mm tunnel length, and 2-step 3.00 mm tunnel length. The Seidel test was evaluated at 5 intraocular pressures (IOPs) in the physiologic range. A masked observer evaluated the recorded tests. With a preset 10 mm Hg IOP, India ink was applied to the incision site and a sudden IOP fluctuation induced. India ink influx was outlined and measured by planimetry.
  
  
  RESULTS
  With the 1.5 mm single-plain incisions, all 6 globes showed inflow and outflow. With the 3.0 mm single-plain incisions, all 6 globes showed inflow and 2 showed outflow by the Seidel test. With the 3.0 mm 2-step incisions, 2 globes showed inflow and 3 had positive Seidel test results. Area and length of inflow were statistically significantly greater with the 2 single-planed incisions than with the 2-step incisions.
  
  
  CONCLUSIONS
  This human ex vivo model showed that wound deformation produced during Seidel testing might not be an accurate way to predict the risk for bacterial invasion in the early postoperative period.
authors.affiliation: Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, California, USA. williamnmaymd@gmail.com
authors.forename: William N
authors.initials: WN
authors.lastname: May
authors.forename: Juan
authors.initials: J
authors.lastname: Castro-Combs
authors.forename: Gilherme G
authors.initials: GG
authors.lastname: Quinto
authors.forename: Renata
authors.initials: R
authors.lastname: Kashiwabuchi
authors.forename: Emily W
authors.initials: EW
authors.lastname: Gower
authors.forename: Ashley
authors.initials: A
authors.lastname: Behrens
mesh_terms: D001082:Aqueous Humor / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D002387:Cataract Extraction / Q000379:methods; D003315:Cornea / Q000378:metabolism / Q000601:surgery*; D003941:Diagnostic Techniques, Ophthalmological / Q000592:standards*; D015818:Eye Infections, Bacterial / Q000175:diagnosis; D019793:Fluorescein; D006801:Humans; D007429:Intraocular Pressure; D008954:Models, Biological; D013529:Surgical Wound Dehiscence / Q000175:diagnosis* / Q000378:metabolism; D014019:Tissue Donors; D014741:Video Recording; D014945:Wound Healing / Q000502:physiology
chemical_list: C028433:chinese ink; D002244:Carbon; D019793:Fluorescein