Identification of up-regulated proteins in the hemolymph of immunized Bombyx mori larvae.
Insects defend themselves against foreign invaders via both a cellular response and a humoral response. The objective of this study was to identify proteins which were differently regulated in the immunized Bombyx mori larvae. Heat-inactivated bacteria (Bacillus megaterium) were injected into B. mori larvae, 4 days after final ecdysis. After 6 h, we identified the immune proteins in the hemolymph which had been differentially regulated in the immune-challenged larvae, using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and quadrupole time-of-flight (Q-TOF) tandem mass spectrometry (MS). After the bacterial injection, more than 30 spots determined to have been up-regulated, and 11 spots were down-regulated. The heat shock 70 kDa protein cognate was one of the up-regulated hemocytic proteins, and peptidoglycan recognition protein, antichymotrypsin precursor, and gloverin-like protein 1 approximately 4 were newly synthesized in the plasma. Antennal binding protein 7 was up-regulated in the plasma. Our results indicated that these immune response proteins were involved with the carrying out of innate immune responses.
Song
Kyung Han
KH
School of Life Science and Biotechnology, Korea University, Seoul 136-701, South Korea.
Jung
Su Jin
SJ
Seo
Young R
YR
Kang
Seok Woo
SW
Han
Sung Sik
SS