[Cloning and expression of new genotype of CYP2C9 from Chinese Han in Escherichia coli].
In this study, we successfully expanded a full length gene encoding the monooxygenase eytochrome P450 2C9 gene from human liver of Chinese Han by RT-PCR. Our findings indicated that except G-->T mutation at the 190th nucleotide site, the other nucleotide sequences are completely consistent with CYP2C9 (NM017460) in GenBank. The SDS-PAGE and Western-Blot analysis showed that the CYP 2C9 gene was successfully expressed in the host cell E. coli BL21 (DE3). Our current study lays the foundation for the evaluation of pre-clinical drug metabolism and safety in the future.
Zhang
Pinghu
P
Jiangsu Center for New Drug Screening, China Pharmaceutical University, Nanjing 210009, China.
Zhang
Juan
J
Gao
Yixiang
Y
Shang
Jing
J
Jiang
Zhenzhou
Z
Zhang
Luyong
L
D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis* / Q000235:genetics*; D002681:China / Q000208:ethnology; D003001:Cloning, Molecular; D065729:Cytochrome P-450 CYP2C9; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D017354:Point Mutation; D011110:Polymorphism, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D011994:Recombinant Proteins; C450260:CYP2C9 protein, human; D065729:Cytochrome P-450 CYP2C9; D001189:Aryl Hydrocarbon Hydroxylases