Dynamic correlation networks in human peroxisome proliferator-activated receptor-γ nuclear receptor protein.
Peroxisome proliferator-activated receptor-γ nuclear receptor (PPAR-γ) belongs to the superfamily of nuclear receptor proteins that function as ligand-dependent transcription factors and plays a specific physiological role as a regulator of lipid metabolism. A number of experimental studies have suggested that allostery plays an important role in the functioning of PPAR-γ. Here we use normal-mode analysis of PPAR-γ to characterize a network of dynamically coupled amino acids that link physiologically relevant binding surfaces such as the ligand-dependent activation domain AF-2 with the ligand binding site and the heterodimer interface. Multiple calculations were done in both the presence and absence of the agonist rosiglitazone, and the differences in dynamics were characterized. The global dynamics of the ligand binding domain were affected by the ligand, and in particular, changes to the network of dynamically correlated amino acids were observed with only small changes in conformation. These results suggest that changes in dynamic couplings can be functionally significant with respect to the transmission of allosteric signals.
Fidelak
Jeremy
J
Structural Biology and Genomics Department, Institut de Génétique et de Biologie Moléculaire et Cellulaire, BP 10413 Illkirch, Strasbourg, France.
Ferrer
Silvia
S
Oberlin
Michael
M
Moras
Dino
D
Dejaegere
Annick
A
Stote
Roland H
RH
D001059:Apoproteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D009068:Movement; D047495:PPAR gamma / Q000819:agonists / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D055550:Protein Stability / Q000187:drug effects; D017434:Protein Structure, Tertiary; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000378:metabolism / Q000494:pharmacology; D014732:Vibration
D001059:Apoproteins; D008024:Ligands; D047495:PPAR gamma; D045162:Thiazolidinediones; D000077154:Rosiglitazone