Anti-virus activity induced by polyinosinic:polycytidylic acid copolymer in in vitro chick embryo fibroblast cells.
The effect of a polyinosinic:polycytidylic acid copolymer (Poly I:C) on chick embryo fibroblast (CEF) cell viability, anti-Newcastle disease virus (NDV) activity and on IFN-alpha and IFN-beta levels were determined in vitro. Poly I:C inhibited NDV growth in CEF cells in the range of 30-500 microg/mL and over 0.75-12 h of treatment.The anti-NDV activity was maximal for 60 microg/mL of Poly I:C treatment at 12 h, after which it declined. The result of VSV protection assay which was used to determine IFN-activity showed that Poly I:C inhibited VSV growing on CEF cells, indicating that Poly I:C had immune-stimulating activities.The amount of IFN-beta in cell supernatant and the transcription levels of IFN-alpha mRNA and IFN-beta mRNA increased after Poly I:C treatment, indicating that the anti-viral activity related to the production of type I IFN. However Poly I:C also showed a toxic effect by inhibiting CEF cell proliferation in a dose-dependent and time-dependent manner.The results of this study indicate that the dosage level of Poly I:C must be carefully considered when used in a clinical setting.
Lv
Yingjun
Y
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Bao
Endong
E
Hartung
Jörg
J
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002642:Chick Embryo / Q000166:cytology* / Q000502:physiology; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology / Q000821:virology; D007370:Interferon Type I / Q000235:genetics; D016898:Interferon-alpha / Q000235:genetics; D016899:Interferon-beta / Q000235:genetics; D011070:Poly I-C / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000998:Antiviral Agents; D017931:DNA Primers; D018076:DNA, Complementary; D007370:Interferon Type I; D016898:Interferon-alpha; D012333:RNA, Messenger; D016899:Interferon-beta; D011070:Poly I-C