A global protein kinase and phosphatase interaction network in yeast.
The interactions of protein kinases and phosphatases with their regulatory subunits and substrates underpin cellular regulation. We identified a kinase and phosphatase interaction (KPI) network of 1844 interactions in budding yeast by mass spectrometric analysis of protein complexes. The KPI network contained many dense local regions of interactions that suggested new functions. Notably, the cell cycle phosphatase Cdc14 associated with multiple kinases that revealed roles for Cdc14 in mitogen-activated protein kinase signaling, the DNA damage response, and metabolism, whereas interactions of the target of rapamycin complex 1 (TORC1) uncovered new effector kinases in nitrogen and carbon metabolism. An extensive backbone of kinase-kinase interactions cross-connects the proteome and may serve to coordinate diverse cellular responses.
Breitkreutz
Ashton
A
Centre for Systems Biology, Samuel Lunenfeld Research Institute, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada.
Choi
Hyungwon
H
Sharom
Jeffrey R
JR
Boucher
Lorrie
L
Neduva
Victor
V
Larsen
Brett
B
Lin
Zhen-Yuan
ZY
Breitkreutz
Bobby-Joe
BJ
Stark
Chris
C
Liu
Guomin
G
Ahn
Jessica
J
Dewar-Darch
Danielle
D
Reguly
Teresa
T
Tang
Xiaojing
X
Almeida
Ricardo
R
Qin
Zhaohui Steve
ZS
Pawson
Tony
T
Gingras
Anne-Claude
AC
Nesvizhskii
Alexey I
AI
Tyers
Mike
M
D001665:Binding Sites; D002244:Carbon / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D004249:DNA Damage; D020935:MAP Kinase Signaling System; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D009584:Nitrogen / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation; D025941:Protein Interaction Mapping; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D020543:Proteome; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D015398:Signal Transduction
C074860:CDC14 protein, S cerevisiae; D018797:Cell Cycle Proteins; D021122:Protein Subunits; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D002244:Carbon; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C500749:target of rapamycin protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases; D009584:Nitrogen