title: Beneficial effect of erdosteine on methotrexate-induced testicular toxicity in mice.
abstract: Methotrexate is used to treat certain types of cancer of the breast, skin, head and neck, or lung. Methotrexate can cause serious or life-threatening side effects on liver, lungs, kidneys, and immune system. Methotrexate chemotherapy causes testicular damage in humans. The aim of this study was to investigate the possible protective role of erdosteine on testicular toxicity of methotrexate in mice. Twenty-six male mice were divided into four groups as follows: group 1, control; group 2, erdosteine-treated; group 3, methotrexate-treated; and group 4, methotrexate + erdosteine treated. On the first day of experiment, a single dose of methotrexate was intraperitoneally administered to groups 3 and 4, although a daily single dose of erdosteine was orally administered to group 2 and 4 for 7 days. At the end of the experiment, the testes of the animals were removed and weighed. The levels of total antioxidant capacity and total oxidative stress, and myeloperoxidase activity in the methotrexate group were higher than the control group (p<0.05). Lipid peroxidation levels were not changed in methotrexate group compared with control group. In conclusion, erdosteine could effectively protect the testes in methotrexate-induced toxicity.
authors.affiliation: Faculty of Medicine, Department of Pharmacology, Mustafa Kemal University, Hatay, Turkey.
authors.forename: Süleyman
authors.initials: S
authors.lastname: Oktar
authors.forename: Ahmet
authors.initials: A
authors.lastname: Gökçe
authors.forename: Mehmet
authors.initials: M
authors.lastname: Aydin
authors.forename: Mürsel
authors.initials: M
authors.lastname: Davarci
authors.forename: Sedat
authors.initials: S
authors.lastname: Meydan
authors.forename: Oktay Hasan
authors.initials: OH
authors.lastname: Oztürk
authors.forename: Ahmet
authors.initials: A
authors.lastname: Koç
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004347:Drug Interactions; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008727:Methotrexate / Q000633:toxicity*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D018709:Statistics, Nonparametric; D013733:Testicular Diseases / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013864:Thioglycolates / Q000494:pharmacology*; D013876:Thiophenes / Q000494:pharmacology*
chemical_list: D013864:Thioglycolates; D013876:Thiophenes; D008315:Malondialdehyde; C048498:erdosteine; D009195:Peroxidase; D008727:Methotrexate