title: Simultaneously entry of palmitic acid into proteolipid protein in different myelin subfractions of rat brain.
abstract: Rats of 20-days of age were injected intracranially with radioactive palmitic acid to study its incorporation into proteolipid protein (PLP) of myelin and myelin subfractions. At short times (120 min), the radioactivity present in PLP was shown to be due to palmitic acid bound to the protein by ester linkages. The specific radioactivity of palmitic acid labeled PLP was identical in all the myelin subfractions except the myelin-like fraction, in which it was lower, suggesting that the entry of the fatty acid into PLP of the different subfractions occurs simultaneously. Experiments using time staggered injections of (14)C- and (3)H-labeled palmitic acid also showed that entry of the fatty acid into PLP of the various subfractions was simultaneous. These results seem to indicate that the acylation of PLP occurs in the myelin membrane and that synthesis and transport of this protein are events unrelated to the acylation process.
authors.affiliation: Programa de Investigación en Neuroquímica (PRINEUQUI), CONICET, Departamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina.
authors.forename: O A
authors.initials: OA
authors.lastname: Bizzozero
authors.forename: E F
authors.initials: EF
authors.lastname: Soto
authors.forename: J M
authors.initials: JM
authors.lastname: Pasquini