Effects of angiotensin II on phosphatidylinositide hydrolysis in rat brain.
Angiotensin II has been coupled to the hydrolysis of phosphatidylinositide in various peripheral tissues; however, little is yet known about its association in the central nervous system. Intracellular phosphatidylinositide was prelabeled by incubation with [(3)H]myo-inositol and the rate of hydrolysis determined by monitoring the rate of formation of [(3)H]inositol monophosphate. In hypothalamus, thalamus, septum and midbrain tissues, angiotensin II reduced the formation of [(3)H]inositol monophosphate and this effect was partially reversed by the presence of an angiotensin II antagonist, sarcosine, isoleucine angiotensin II. These data suggest that angiotensin II receptor stimulation may inhibit the hydrolysis of phosphatidylinositide in the brain.
Tamura
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Department of Pharmacology/Toxicology, Washington State University, Pullman, WA 99164-6520, U.S.A.
Speth
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RC