title: Potato virus X: structure, disassembly and reconstitution.
abstract: SUMMARY This paper summarizes some structural characteristics of Potato virus X (PVX), the flexuous filamentous plant potexvirus. A model of PVX coat protein (CP) tertiary structure in the virion proposed on the basis of tritium planigraphy combined with predictions of the protein tertiary structure is described. A possible role of glycosylation and phosphorylation in the CP structure and function is discussed. Two forms of PVX virion disassembly are discussed: (i) the virion co-translational disassembly after PVX CP in situ phosphorylation and (ii) disassembly of PVX triggered by different factors after linear destabilization of the virion by binding of the PVX-coded movement protein (TGBp1) to one end of the polar CP-helix. Special emphasis was placed on a translational activation of encapsidated PVX RNA and rapid disassembly of TGBp1-PVX complexes into free RNA and CP. The results of experiments on the PVX CP repolymerization and PVX reconstitution are considered. In particular, the products assembled from PVX RNA, CP and TGBp1 were examined. Single-tailed particles were found with a helical, head-like structure consisting of helically arranged CP subunits located at the 5'-tail of RNA; the TGBp1 was bound to the end of the head. Translatable 'RNA-CP-TGBp1' complexes may represent the transport form of the PVX infection.
authors.affiliation: Department of Virology and A.N. Belozersky Institute of Physico-Chemical Biology of Moscow State University, Moscow 119992, Russia.
authors.forename: Joseph
authors.initials: J
authors.lastname: Atabekov
authors.forename: Eugen
authors.initials: E
authors.lastname: Dobrov
authors.forename: Olga
authors.initials: O
authors.lastname: Karpova
authors.forename: Nina
authors.initials: N
authors.lastname: Rodionova