title: Genetic polymorphisms among C57BL/6 mouse inbred strains.
abstract: Mice from the inbred C57BL/6 strain have been commonly used for the generation and analysis of transgenic and knockout animal models. However, several C57BL/6 substrains exist, and these are genetically and phenotypically different. In addition, each of these substrains can be purchased from different animal providers and, in some cases, they have maintained their breeding stocks separated for a long time, allowing genetic differences to accumulate due to individual variability and genetic drift. With the aim of describing the differences in the genotype of several C57BL/6 substrains, we applied the Illumina(®) Mouse Medium Density Linkage Mapping panel, with 1,449 single nucleotide polymorphisms (SNPs), to individuals from ten C57BL/6-related strains: C57BL/6JArc, C57BL/6J from The Jackson Lab, C57BL/6J from Crl, C57BL6/JRccHsd, C57BL/6JOlaHsd, C57BL/6JBomTac, B6(Cg)-Tyr ( c-2j )/J, C57BL/6NCrl, C57BL/6NHsd and C57BL/6NTac. Twelve SNPs were found informative to discriminate among the mouse strains considered. Mice derived from the original C57BL/6J: C57BL/6JArc, C57BL/6J from The Jackson Lab and C57BL/6J from Crl, were indistinguishable. Similarly, all C57BL/6N substrains displayed the same genotype, whereas the additional substrains showed intermediate cases with substrain-specific polymorphisms. These results will be instrumental for the correct genetic monitoring and appropriate mouse colony handling of different transgenic and knockout mice produced in distinct C57BL/6 inbred substrains.
authors.affiliation: Department of Molecular and Cellular Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus de Cantoblanco, Darwin 3, 28049, Madrid, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Zurita
authors.forename: Mónica
authors.initials: M
authors.lastname: Chagoyen
authors.forename: Marta
authors.initials: M
authors.lastname: Cantero
authors.forename: Rosario
authors.initials: R
authors.lastname: Alonso
authors.forename: Anna
authors.initials: A
authors.lastname: González-Neira
authors.forename: Alejandro
authors.initials: A
authors.lastname: López-Jiménez
authors.forename: José Antonio
authors.initials: JA
authors.lastname: López-Moreno
authors.forename: Carlisle P
authors.initials: CP
authors.lastname: Landel
authors.forename: Javier
authors.initials: J
authors.lastname: Benítez
authors.forename: Florencio
authors.initials: F
authors.lastname: Pazos
authors.forename: Lluís
authors.initials: L
authors.lastname: Montoliu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D005260:Female; D005838:Genotype; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics*; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity